LOCUS       DAYYAO010000001        68232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000001 DAYYAO010000000
VERSION     DAYYAO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.001"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..920
                     /locus_tag="ACHARI_000001"
     CDS             39..920
                     /locus_tag="ACHARI_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8441088.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            933..2543
                     /locus_tag="ACHARI_000002"
     CDS             933..2543
                     /locus_tag="ACHARI_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441089.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2620..3279)
                     /locus_tag="ACHARI_000003"
     CDS             complement(2620..3279)
                     /locus_tag="ACHARI_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8441090.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3562..4227)
                     /locus_tag="ACHARI_000004"
     CDS             complement(3562..4227)
                     /locus_tag="ACHARI_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8441091.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4374..4979)
                     /gene="dhaL"
                     /locus_tag="ACHARI_000005"
     CDS             complement(4374..4979)
                     /gene="dhaL"
                     /locus_tag="ACHARI_000005"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8441092.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5007..6017)
                     /gene="dhaK"
                     /locus_tag="ACHARI_000006"
     CDS             complement(5007..6017)
                     /gene="dhaK"
                     /locus_tag="ACHARI_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8441093.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5992..6387)
                     /gene="dhaM"
                     /locus_tag="ACHARI_000007"
     CDS             complement(5992..6387)
                     /gene="dhaM"
                     /locus_tag="ACHARI_000007"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8441094.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6541..6861
                     /locus_tag="ACHARI_000008"
     CDS             6541..6861
                     /locus_tag="ACHARI_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8441095.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6973..7830)
                     /locus_tag="ACHARI_000009"
     CDS             complement(6973..7830)
                     /locus_tag="ACHARI_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8441096.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7938..9242)
                     /locus_tag="ACHARI_000010"
     CDS             complement(7938..9242)
                     /locus_tag="ACHARI_000010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8441097.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9239..9748)
                     /locus_tag="ACHARI_000011"
     CDS             complement(9239..9748)
                     /locus_tag="ACHARI_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441098.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9923..12058)
                     /locus_tag="ACHARI_000012"
     CDS             complement(9923..12058)
                     /locus_tag="ACHARI_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8441099.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12141..13796)
                     /locus_tag="ACHARI_000013"
     CDS             complement(12141..13796)
                     /locus_tag="ACHARI_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8441100.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13798..14112)
                     /locus_tag="ACHARI_000014"
     CDS             complement(13798..14112)
                     /locus_tag="ACHARI_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8441101.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14282..14542
                     /locus_tag="ACHARI_000015"
     CDS             14282..14542
                     /locus_tag="ACHARI_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8441102.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14556..14870
                     /locus_tag="ACHARI_000016"
     CDS             14556..14870
                     /locus_tag="ACHARI_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8441103.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15600..16154)
                     /locus_tag="ACHARI_000017"
     CDS             complement(15600..16154)
                     /locus_tag="ACHARI_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8441104.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16280..18209)
                     /locus_tag="ACHARI_000018"
                     /pseudo
     CDS             complement(16280..18209)
                     /locus_tag="ACHARI_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18515..19231)
                     /locus_tag="ACHARI_000019"
     CDS             complement(18515..19231)
                     /locus_tag="ACHARI_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8441105.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19232..21055)
                     /gene="glpO"
                     /locus_tag="ACHARI_000020"
     CDS             complement(19232..21055)
                     /gene="glpO"
                     /locus_tag="ACHARI_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8441106.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21069..22565)
                     /gene="glpK"
                     /locus_tag="ACHARI_000021"
     CDS             complement(21069..22565)
                     /gene="glpK"
                     /locus_tag="ACHARI_000021"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8441107.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22701..24161)
                     /locus_tag="ACHARI_000022"
     CDS             complement(22701..24161)
                     /locus_tag="ACHARI_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441108.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24151..25446)
                     /locus_tag="ACHARI_000023"
     CDS             complement(24151..25446)
                     /locus_tag="ACHARI_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8441109.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25778..26455)
                     /locus_tag="ACHARI_000024"
     CDS             complement(25778..26455)
                     /locus_tag="ACHARI_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8441110.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26485..27576)
                     /locus_tag="ACHARI_000025"
     CDS             complement(26485..27576)
                     /locus_tag="ACHARI_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8441111.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27677..28111)
                     /locus_tag="ACHARI_000026"
     CDS             complement(27677..28111)
                     /locus_tag="ACHARI_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8441112.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28695..30152)
                     /locus_tag="ACHARI_000027"
     CDS             complement(28695..30152)
                     /locus_tag="ACHARI_000027"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8441113.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30149..32044)
                     /locus_tag="ACHARI_000028"
     CDS             complement(30149..32044)
                     /locus_tag="ACHARI_000028"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8441114.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32102..33877)
                     /locus_tag="ACHARI_000029"
     CDS             complement(32102..33877)
                     /locus_tag="ACHARI_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8441115.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33927..35423)
                     /locus_tag="ACHARI_000030"
     CDS             complement(33927..35423)
                     /locus_tag="ACHARI_000030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8441116.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35617..37239)
                     /locus_tag="ACHARI_000031"
     CDS             complement(35617..37239)
                     /locus_tag="ACHARI_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8441117.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37567..38880)
                     /locus_tag="ACHARI_000032"
     CDS             complement(37567..38880)
                     /locus_tag="ACHARI_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8441118.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39125..39349)
                     /locus_tag="ACHARI_000033"
     CDS             complement(39125..39349)
                     /locus_tag="ACHARI_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441119.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39369..39791)
                     /locus_tag="ACHARI_000034"
     CDS             complement(39369..39791)
                     /locus_tag="ACHARI_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8441120.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39933..40643)
                     /locus_tag="ACHARI_000035"
     CDS             complement(39933..40643)
                     /locus_tag="ACHARI_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8441121.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40661..41158)
                     /locus_tag="ACHARI_000036"
     CDS             complement(40661..41158)
                     /locus_tag="ACHARI_000036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8441122.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41195..41647)
                     /locus_tag="ACHARI_000037"
     CDS             complement(41195..41647)
                     /locus_tag="ACHARI_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8441123.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41782..42387)
                     /locus_tag="ACHARI_000038"
     CDS             complement(41782..42387)
                     /locus_tag="ACHARI_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8441124.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42837..43457)
                     /locus_tag="ACHARI_000039"
     CDS             complement(42837..43457)
                     /locus_tag="ACHARI_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8441125.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43459..44328)
                     /locus_tag="ACHARI_000040"
     CDS             complement(43459..44328)
                     /locus_tag="ACHARI_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8441126.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44351..44758)
                     /locus_tag="ACHARI_000041"
     CDS             complement(44351..44758)
                     /locus_tag="ACHARI_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8441127.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44777..46066)
                     /locus_tag="ACHARI_000042"
     CDS             complement(44777..46066)
                     /locus_tag="ACHARI_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8441128.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46093..46395)
                     /locus_tag="ACHARI_000043"
     CDS             complement(46093..46395)
                     /locus_tag="ACHARI_000043"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8441129.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46420..48513)
                     /locus_tag="ACHARI_000044"
     CDS             complement(46420..48513)
                     /locus_tag="ACHARI_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8441130.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49002..49766)
                     /locus_tag="ACHARI_000045"
     CDS             complement(49002..49766)
                     /locus_tag="ACHARI_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8441131.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49877..50746)
                     /gene="mdcB"
                     /locus_tag="ACHARI_000046"
     CDS             complement(49877..50746)
                     /gene="mdcB"
                     /locus_tag="ACHARI_000046"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8441132.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50771..51847)
                     /locus_tag="ACHARI_000047"
     CDS             complement(50771..51847)
                     /locus_tag="ACHARI_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8441133.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51871..52464)
                     /locus_tag="ACHARI_000048"
     CDS             complement(51871..52464)
                     /locus_tag="ACHARI_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8441134.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52468..54144)
                     /gene="mdcD"
                     /locus_tag="ACHARI_000049"
     CDS             complement(52468..54144)
                     /gene="mdcD"
                     /locus_tag="ACHARI_000049"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8441135.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54134..54433)
                     /locus_tag="ACHARI_000050"
     CDS             complement(54134..54433)
                     /locus_tag="ACHARI_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8441136.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54535..56181)
                     /gene="mdcA"
                     /locus_tag="ACHARI_000051"
     CDS             complement(54535..56181)
                     /gene="mdcA"
                     /locus_tag="ACHARI_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM8441137.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56188..57099)
                     /locus_tag="ACHARI_000052"
     CDS             complement(56188..57099)
                     /locus_tag="ACHARI_000052"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8441138.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57219..58076
                     /locus_tag="ACHARI_000053"
     CDS             57219..58076
                     /locus_tag="ACHARI_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8441139.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58263..58625)
                     /locus_tag="ACHARI_000054"
     CDS             complement(58263..58625)
                     /locus_tag="ACHARI_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8441140.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58814..58996)
                     /locus_tag="ACHARI_000055"
     CDS             complement(58814..58996)
                     /locus_tag="ACHARI_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441141.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            58971..60410
                     /locus_tag="ACHARI_000056"
     CDS             58971..60410
                     /locus_tag="ACHARI_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441142.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60417..62969)
                     /locus_tag="ACHARI_000057"
     CDS             complement(60417..62969)
                     /locus_tag="ACHARI_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8441143.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63083..64414)
                     /locus_tag="ACHARI_000058"
     CDS             complement(63083..64414)
                     /locus_tag="ACHARI_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8441144.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64380..65858)
                     /locus_tag="ACHARI_000059"
     CDS             complement(64380..65858)
                     /locus_tag="ACHARI_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8441145.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66163..67998)
                     /gene="tdc"
                     /locus_tag="ACHARI_000060"
     CDS             complement(66163..67998)
                     /gene="tdc"
                     /locus_tag="ACHARI_000060"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8441146.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19405 a        14561 c        11676 g        22590 t
ORIGIN      
        1 gtttagtata tatttttaga aaaaggagtg gggtttttat ggcagaaatc gtcaaagtca
       61 cacatttgaa aaagagcttt ggtcgtttcg aagctttaaa agatgtctct ttcaccgtac
      121 attcaggtga agtcgttgga tttatcggac caaatggttc tggaaaatcc acgacgattc
      181 ggacactttt agggattata aaaaaagacg gcggaacggc aacgattttt gggcaagatg
      241 tatgggaaga tagtttatct atccatgaac gtctttctta tgttccaggc gatatctctt
      301 tgtggggaaa tttgactgga ggagcgatca tcgatctctt catgaagctt catggaaaag
      361 ggagtgctca aaaaaaagat gaactgattc aacgtttctc tcttgatcct aagaaaaaag
      421 caaaaaacta ttcaaaagga aatcgccaaa aagtgggctt gattgctgct ttggctgttg
      481 attcggatct ctatttattt gatgagccta cttctggttt agatcctttg atggaaacga
      541 ttttccaaga agaagttgaa aaattgaaag aacagggcaa atccatccta ctttcttctc
      601 atattctcag tgaagtcgag cgactagcgg ataaagtcgt gatcatacgc gaaggaacac
      661 tcgatgaaga aggaacactc gatgaattgc gtcatttgac tcgttcgacc atcactatag
      721 aaacagaagc agagaatagt cacttagcac aattgaacgg tgtccatgac tttgtgcaaa
      781 aaggtcacct cgctcaattt gctgctgata acgaagcttt cgatgtgatt ttagctgaag
      841 cgtccaaatt aggtgtgaaa aaatttgaat ctattccacc gactcttgag gatttattca
      901 tgagacatta tgaaggatga ggaggaagcg aaatgactga taaattttca cgctggtcca
      961 cccttttttc tcaaaacctt agccgtgact ggaaaaagat cctgatatgg ctagtcggtg
     1021 tcactgcttt ttctggtggt tttgtccctg cgtttgaaga attgacttcc ggacaaggat
     1081 tagctggatt atacgaaacc ttgcaaaacc cggcaatgat tgcaatggtc ggaccaactc
     1141 cagcttcgaa tgctgctgag tatactttag gagcgatgta tgcaaatgaa atgttgcttt
     1201 tttgcagttt gtttgctgct gtgatctctg ttttacacgt ggtgagccaa acgagaaaaa
     1261 aagaagaact tggtctgatt gaacttattc gttcctttcg agtcggacgt caagcaaact
     1321 ctttagctgt catcgctgaa agtattctga tcaatttgct tttagcaata tcgatcagct
     1381 gtacgatgat ttttttccaa gcggattcga tcactgcaga aggtgcttgg ctttttgggc
     1441 tatctgtagg tagtatgggg attttgggga gtgccgttgc actgtttgct gcacaactga
     1501 tgccaagttc agccagttcg aatggtctgt ctttagcgtt tttaggtgga ctttaccttc
     1561 tacgagcaat aacagatgta gctgattcct ccctttcgat gtggaatcct ttaggctggt
     1621 cttatttgac ctatccattt acaactaaca actggattcc tttgctttat ttgtttttgt
     1681 tcagtctaat cttgataatc agttcatttg ttttagagga acatcgagat ttgaacaccg
     1741 gctatatccc cgaacgtgaa ggaaaaccgt atgctaaacg ttctctttta tccttgcgtg
     1801 gtttattatt ctatctaaat aaagggatga caatcagttg gcttatcact tttggtttga
     1861 tgggggcggc atacggctct atctacggga atatgcaaca gtttttagaa agtaacgact
     1921 taatgaaaca gatgtttatc caatctggcg tctcaattga aaaaaacttt acaagcacga
     1981 tcatggtagt cttgatttgt ctagctgcta ttttaccaat tgctagcatc aatcgtttat
     2041 ttacagaaga aacccacgga cgtatgaatc agctgttcgc aacaaaaatt tctaggaacc
     2101 aactttattg gaccaatatg ttactagcta ttctatttgc tatattggca atcctgatta
     2161 gttctggtgc actagggctt tctgctttgg cttcgatgaa ggatgcgaaa gacatggatc
     2221 tattgatgtt tcttaaagcc gggttcaatt tcctgcctgc cgttttagtg tttatcggaa
     2281 ttagtggctt tctattcgga tggcttcctc gaatcggtaa acttgcttat atctacttag
     2341 cttactcctt tgcactcaat tactttggcg gaattttgga tttaccagaa tggttttcca
     2401 aaacagctgt ccctagctgg gttccacgga tgccaaacga ttcttttgat ctttccgtgt
     2461 tccttacgtt atcatgtatc agccttgcac tgttcgtcct tggttctatt ggttataggc
     2521 gaagagactt actagaagga taataaaaaa gcatgtacaa tttcatgcta gcctccaaat
     2581 ctgggtcaaa aatccaacat ttggaggtca tttttttatt tacaagtctt ctaactgctc
     2641 tttatcaatg atccagactt ctcgattctt tctttttaca taaccattct cctctaataa
     2701 tttaaatttt ctagataaag tttcgggggt cgtaccgata aatgcagcaa gttctttcat
     2761 tttcattggc aggaaaaagt gatccttccc tgtcgcttta gctaagtcaa gcagatagac
     2821 agcgactcgt tcttctactt tttccatcat caaaaaagtt gtctgctgct gtacttttgt
     2881 taatttctca acgttgattg ctaaaagttt cagactaagg gcgggataat gcagtaagag
     2941 ttccgagaaa tctttttgtc tcaatatgca aatttccgtt ttttctagtg cttcgccaaa
     3001 taaagactca tttggaacgc ctagcaactg gctctctccc tcatatccgc ccggctctat
     3061 tactcgtaaa agctgttcct ttccgctagc agataattga tacactttca tactgccgga
     3121 ggccacgatc actaattgag gttctccatt cggcgcaaga atctgttctc ctttttccac
     3181 atagtgatgt ttgacaagtg cgtgtacttt ttcttgatct tgctgtacta agtgattgaa
     3241 caaaggaaca aatgaaacac ataaatgatc atgactcata atctctcctc cttctaaaca
     3301 gctatttcta ataaagaaac ataacttccc ttattccaac tgataatttt cttttatcat
     3361 agcataaacg tcaagcgaac cagataccag caaatagagt ggatttcttt ttgtcccttg
     3421 ctttattttc cttcttttga gaccttaagt aattgattcc tatcaatttt ttaaatattt
     3481 actttttgaa taatcaaaaa caaaccttca ctcgcaagcc gagagtaagg tttgtttttc
     3541 gcattgctta tttcgtgttg tctattgaaa tttttccgca cctagctttt gatatagtgg
     3601 tgctagttct tgtaaggttt cagcggttat ttgctcaacg tgttcgattt cttgcggata
     3661 gaagaatagt ttacccattt gaaaggttcg gtctctcatc gcttttttca actcttcttg
     3721 tttctttgta tcggacaaat agtcagacaa agctaagtgg tcgtcaagtt catgttctgg
     3781 ttgcggccat atgtaataat ccttgatatc gactgttttg ctccactctt tcagtatcgc
     3841 aagcaactgg ttatacgctg caatgctacc atatcgttct tcgcttttat aatgttggta
     3901 catcaaatag atttggtatt gtttttgatt caacaaaact gctagacagg cattctcgtc
     3961 ttgacgagat tcgcttcggt acgcgctcca aaaatgactg cgtaaattcc agccattcgt
     4021 ccagctttcg attttaggtt tgctcatgcc gtacatatca ggcaaaagtg cagctgtctg
     4081 gagctgaatc tctttccatt tttgccagtg ttctttatat tctttcttga tttgttcaat
     4141 cttatctgtt ggatggctct ctttgagctg tttgaatgcg tagattggtc gatcaaacaa
     4201 ctgataatct gattttccaa gttccattgt ttcgcctctt tccgttatct tttgtattct
     4261 acctgaattt tgcgatatgg acaaatagag gttgtgaaaa agctgttctg catcaagtca
     4321 taagaacagc caaacaaaaa actgcgatga gcatcaccgc agttttattg aacttaaggt
     4381 tgattgatca catctaaaag cgttgaaaat aaaatggcac tagaagcagc tcctgggtcg
     4441 atatgtccga ttgcacgctc tcctagatag gaagctcgtc cttttttagc taccaagact
     4501 ttggtgtgtt cttttgcctg gtcaatgatt tcttttgtta aattccctgc cttcaatgcc
     4561 tctgtgactg gcagccaaac atctaacatc gttttatcgt cagcttctgc ctttcctcgg
     4621 ctttgaatac ccgctgttcc ctgctcgatc acttcgccta atttttcttg agaatcaata
     4681 gaatcgacct cttttgtcgc tttcatcatg ttcatgaatg cagatccgta taacggacca
     4741 gaagccccac cgactttcga tatcatcgcc atagagaata ctttgaatgt atcgctgatt
     4801 gtttcaggat gatcggcttg aaaagcttct tcataagctg aaacccctct tcccatgtta
     4861 tttccatggt ctccatcgcc aatcggggta tctagatcac tgagatattg tttgttctga
     4921 ttgattgcct ctgcaaattg cgtgagccat tcttggattt cttgaacggt tagtttcata
     4981 ttatcaccct tttatcttga gattttttac catgaaatcg tctgtacatt gctttctaaa
     5041 gaatctttcc attgtgtctc cgtcaagtcg atcagtgtta gagaaagacc ttgcatatct
     5101 agtgaggtca taaaattacc gacttttcgg aaaactacat tgacgcctct ttcttccaac
     5161 agagctaaaa catcattcat gaacacgaac tgttccatca aaggcgtccc gcccattccg
     5221 ttgactagta cacctgcttc ttttggtaaa ttcgagtaat catctaaaat cttagtaaca
     5281 agttcctttg ccagtacttt agaaggctgc atcttttctc gacgatagcc tggctcaccg
     5341 tggataccaa caccatactc gatctcatct tctcctagtt cgaatcctgg tttccctact
     5401 tctggtacag ttgctgctcg aagcgctaga ccgatcgtct tagttgcctg tacgaccttt
     5461 tctcccagtt ctttcaactc agctagggaa gcaccgtttc ttgctgcatc gcccacgatt
     5521 ttgtgtacaa ggacagtacc tgcaactccg cgttttccag cagtatacgt actattttca
     5581 acggcgatat catcatctac gatcacactt tctacttcaa tatcgtccat agcagctaat
     5641 tcctttgcca tatcgaagtt caatgcatcc cctgtataat tcttaacgat cagcaaaact
     5701 ccttttccac tatcggcttc cttaatagcc gtctgaatct gatctggagt tggagaagta
     5761 aacacatcac caagcaccgc tgcacttaac atcccttccc ctacaaaccc ggcatgggat
     5821 ggttcatgtc cgcttcctcc accagaaacc aaaccaactt gttccgcttt ctgattcttg
     5881 gctacaacac gggaagattc tacacgatga acaagttctg gatagctttt tactaatcct
     5941 tccagcattt cttcgacaat attgcttgga tcattcatga tttttttcaa tttattccca
     6001 tcctttcttt ccggcatttt gtgcttctgt taagatttgc tgagtatcat ctgttatacc
     6061 ggcagtgatg gctgctgcaa aagctccttc cactaaaggc gcatccacca gatgataacg
     6121 ttgctgctgc tcttcttcca acatgtcttt tgccagttcc gagttcaata ccgcgctacc
     6181 gatatccgca aaaacaaaat aactagctgc atcgcttgat tcattgactg cttctacgat
     6241 tttcatcggg tctgatccga tctcgccatt tattccgccg agagaaaaaa tttgcacctc
     6301 ttcggatttt tgcatctgct caatcatttc tttaatacca tctgttactt tttgactatg
     6361 agaaataagc aaaatacttt ttttcacaag aatcttcctt tcttttacat agttgctaac
     6421 gattggtttc gctttcatta atgaacaatt caagtatata ataaatttct aattttggga
     6481 aaatatgtgt agtgcttctt gaagtttgtt atactttaat taaaagagag gagtgatcga
     6541 atgattacaa caacaacgaa cagtgtagag ggaaaagccg tagtagctta taaagggatc
     6601 gtttttggtg aggtcattac tgggatccat gcatttaaag acttggaggc ggggttgcga
     6661 aatatctttg gcggccgctc aaaaagctat gaaaacgaac taatgggtgc aagagaagaa
     6721 gcacttgccg aaatggccga acgtgcaaaa gcattaggag ctgatgcgat cattggcgta
     6781 aaaatggact atgaagtatt aggcagcgac aatggcatgc tgatggtcac atgtagtgga
     6841 acagctgtct ccacgagata agtaggggac atctgtttta ataactaaag agagatggga
     6901 catgtagtgg cactcaagaa ttagattttt cagtctaact tcgaggtact ctaccttccc
     6961 atcctctctt ttttatttct ttttgcggat gattccgtaa gctgctgcga ctaacacacc
     7021 cgcccctgct aaggaataac tgacagaagc tgttttttca tttgtttttg gcaaagtctg
     7081 acttgttgat ttggatggct ctgtcgtttg ctgaactgtt tctgtcgtgc ttccagaggt
     7141 cactgatttt tctcctgccg aatgcaaaat atcttttatt cgttggaaac tagctgctaa
     7201 gtcagctttt tttgcttcaa aatcattagc aactgtttgt gcttcgctga tctttccgtg
     7261 gattgcttga ctggccgctg tgatatccgc ttgagtttga cttagctttc cagaagctgt
     7321 ttgagcaacc tgatttaatt gctgattgaa ttcttttgct tgttggatct tcccgtcaag
     7381 atctgtttgg acagcttccg cttttgcttt cgcttcggca agctttttct caagttgtgc
     7441 acggtcattt cccactttat ttttaagatc agctaaatct tgatcgattg ctgcttggtc
     7501 ttctttcaat ttgttttttg cagcttctaa tgcttcttcg agttcttttc ttttattttc
     7561 caagtcttgc aaatgatcga tttggtcaga agaggtagaa ctatttattg atgtatcttc
     7621 tgaagatgaa atcgtttctc ctgaattatc tgtcggtaca ttggaagtca tttctgtttc
     7681 cgaggcaccg ttttcagcac cagaagagct tatggttgtt gaaggttctg tttgagcagg
     7741 ttcagaagtt tccgcaaaag ctgtctgtcc caataagaaa atactggaaa cagcaattgg
     7801 ccagattaac ggcttcattc tttttttcat cacttttccc atccttacta ctttttaact
     7861 aatttcttat tataagcaaa aatgtttgga agaaccataa acacaacagg ctgtttgtct
     7921 aaaaaacggt gcatctttta tttttttgta cgttccgggc ttttccatgt cccgcttcca
     7981 taacctaaaa tcgttttgac tgctggaatg aaagtcgtca cgatcgttat cgcgttcact
     8041 atccagaaca aaagcatata tagtggtgcg aaaagaaggt atttcaattt tcttcgtcga
     8101 tcatctacaa tcagcgaagc tagcaactgg aaaaatcctg cgaccatttc aaagcagatg
     8161 aacacaaaac tgaatgtaaa agtaatataa atctgttcgt agtttccttg atatacataa
     8221 tgcccgatta atccagcaaa aagaactacg gaaatacaaa agaatattga ccagacaata
     8281 ctcaatgttt gatcaatgaa gatgatcgtt cttccaatat gtttaaaagg atggagcatc
     8341 acttttttga aattcgtcag ccagacttct gttccacctt ttgcccaacg ttttctgtgt
     8401 cgatatagca tcttcaacgt cacaggtact tccatgaaaa agataatctc aggagcaaaa
     8461 acagataacc agtcatttaa ctgatgatcc caagcaatac tgatgtcttc tgtcgctcga
     8521 tcttgtctga atccgcccac atcgatcaag gcttcttttc gatacatcgt atttgctcca
     8581 ctgtacgcat aaagaccacc gaaaacagct gactgtgttc gtttaatgat gcccacgata
     8641 cttgagaatt caaccgtttg agattttgca atcaatttgg tacgattttg gacatccata
     8701 tttgcagtga ccgcagcaat atgtcttgca ccagggcgaa tgaaataatt cacataccta
     8761 atcagtgcat ctggctccgg taccgtgtct gcatcattgc ttaaaatcaa tttccctttt
     8821 gcaaaggcaa gtccgatatt aaacgcgtgg gcttttcctt tatttttttc gatccgaacc
     8881 acacgaagat tagcgtattt tttcatcagt ctagctaata tttctggtgt ttggtccgtt
     8941 gaaccatcgt ctgtcactaa tacctcatag ttgtgataat tcaatttcgt catcaaatac
     9001 tcgattgtat attcgatcac aatttcttca ttatgggctg gcaccatgat cgtgatgaac
     9061 ggttcgacac tcaagggaac atctacccat tctttttgct tatgtttgta taaaaacaca
     9121 taacaccaga caccaataaa ccatacaaat ccgcctaaaa tcggataaga aacgagaaaa
     9181 aaatacataa tagacaaaca agtatctaag aataaattac ctgtatgggt gattgagatc
     9241 atttcagttc caccccattt tctttataca aatctgcaat gaatgtcgta tctagatttt
     9301 tctcttcgga gacagaataa aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt
     9361 ccgtatacac ttcttcaagt aattcttgtc tttttttcaa tttttcatct tcatattcaa
     9421 aagatttatt tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg
     9481 taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct
     9541 tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga
     9601 aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt
     9661 gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg
     9721 caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta
     9781 ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa
     9841 aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt
     9901 ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc
     9961 ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa
    10021 atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt
    10081 ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct
    10141 cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta
    10201 gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg
    10261 gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg
    10321 tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt
    10381 agtcgtcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat
    10441 tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt
    10501 gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta
    10561 ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg
    10621 tgggttgcca agcataagct gagccatatc gtttttcata aattctttca aacaactgat
    10681 gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta
    10741 aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga
    10801 ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt
    10861 ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa
    10921 gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt
    10981 tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa
    11041 acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct
    11101 gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct
    11161 ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta
    11221 atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca
    11281 agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga
    11341 tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat
    11401 cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg
    11461 tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa
    11521 aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat
    11581 cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt
    11641 cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg
    11701 ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc
    11761 caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct
    11821 gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca
    11881 ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact
    11941 ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta
    12001 gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac
    12061 acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa
    12121 tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt
    12181 tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt
    12241 ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg
    12301 tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat
    12361 ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt
    12421 tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct
    12481 gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat
    12541 tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg
    12601 atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct
    12661 ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct
    12721 tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt
    12781 aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct
    12841 tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct
    12901 ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca
    12961 tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc
    13021 ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca
    13081 atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc
    13141 ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac
    13201 aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct
    13261 tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg
    13321 atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact
    13381 ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga
    13441 atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg
    13501 tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact
    13561 tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca
    13621 agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca
    13681 aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga
    13741 agccgtgtag cagcagacat tccacctgct acacctccga caataacgat tttcatctta
    13801 tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc
    13861 ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat
    13921 cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt
    13981 tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc
    14041 tggattttcc aaaagctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa
    14101 tagcaaattc attttctgca cctcatttac atttttataa atacctcatg gggtatatta
    14161 aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta
    14221 tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt
    14281 tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg
    14341 tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag
    14401 cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa
    14461 ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat
    14521 caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg
    14581 aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag
    14641 aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca
    14701 atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg
    14761 ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc
    14821 ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg
    14881 ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct
    14941 ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc
    15001 aaacaaaaat agcttctcat atttttcgtt tggttgggct tattaccgca gatgcaagct
    15061 tttgaacacc gtttaaggaa gaggttgtga aatcagcggt ttgacctaag tattagagaa
    15121 aaaaacggga aaatagctcc tcatattttg ccgttttttg agctaatacc gaaggtcagc
    15181 tgattgaaca ccgtttaagg aagagattgt gaaaaagtcg cttagtttcg agtattaaga
    15241 aaaattttgt gaaaatggat ttctacattt ttgcataaat ttggcttaat accgagaaac
    15301 tggcttttga ataccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta
    15361 gagaaacaaa cggaaaaata gctgctcata ttttgccgtt ttttgagcta ataccgaagg
    15421 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac
    15481 aagaaataat ttcaaaaaca acttctcctg tttttggttc ggttggctta ttatcgagaa
    15541 gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt
    15601 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
    15661 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
    15721 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
    15781 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
    15841 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
    15901 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
    15961 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
    16021 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
    16081 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
    16141 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
    16201 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacaga cactgatatt
    16261 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
    16321 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
    16381 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
    16441 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
    16501 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
    16561 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctgacgttct tttttattct
    16621 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
    16681 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
    16741 caatccacaa agcttggctt tctcctgttt cttccaaccc gcaactgata tttaggacga
    16801 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
    16861 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
    16921 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
    16981 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
    17041 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
    17101 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
    17161 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
    17221 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
    17281 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
    17341 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
    17401 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
    17461 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
    17521 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
    17581 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
    17641 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
    17701 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
    17761 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
    17821 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
    17881 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
    17941 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
    18001 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
    18061 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
    18121 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
    18181 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
    18241 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
    18301 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
    18361 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
    18421 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
    18481 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
    18541 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
    18601 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
    18661 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
    18721 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
    18781 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
    18841 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
    18901 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
    18961 tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
    19021 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
    19081 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
    19141 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
    19201 cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
    19261 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
    19321 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
    19381 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
    19441 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
    19501 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
    19561 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
    19621 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
    19681 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
    19741 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
    19801 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
    19861 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
    19921 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
    19981 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
    20041 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
    20101 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
    20161 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
    20221 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
    20281 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
    20341 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
    20401 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
    20461 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
    20521 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
    20581 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
    20641 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
    20701 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
    20761 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
    20821 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
    20881 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
    20941 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
    21001 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
    21061 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
    21121 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
    21181 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
    21241 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
    21301 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
    21361 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
    21421 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
    21481 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
    21541 tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt
    21601 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
    21661 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
    21721 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
    21781 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
    21841 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
    21901 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
    21961 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
    22021 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
    22081 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
    22141 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
    22201 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
    22261 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
    22321 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
    22381 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
    22441 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
    22501 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
    22561 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
    22621 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
    22681 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
    22741 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
    22801 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
    22861 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
    22921 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
    22981 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
    23041 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
    23101 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
    23161 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
    23221 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
    23281 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
    23341 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
    23401 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
    23461 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
    23521 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
    23581 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
    23641 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
    23701 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
    23761 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
    23821 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
    23881 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
    23941 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
    24001 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
    24061 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
    24121 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
    24181 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
    24241 ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat
    24301 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
    24361 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
    24421 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
    24481 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
    24541 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
    24601 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
    24661 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
    24721 ttcccacatc tgattgtcgg gacgagaatg attagccagc aaaacaagat catagttctc
    24781 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
    24841 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
    24901 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
    24961 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
    25021 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
    25081 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
    25141 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
    25201 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
    25261 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
    25321 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    25381 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    25441 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    25501 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    25561 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    25621 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    25681 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    25741 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    25801 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    25861 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    25921 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    25981 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    26041 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    26101 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    26161 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    26221 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    26281 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    26341 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    26401 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    26461 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    26521 gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc
    26581 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    26641 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    26701 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    26761 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    26821 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    26881 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    26941 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    27001 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    27061 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    27121 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    27181 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    27241 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    27301 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    27361 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    27421 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    27481 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    27541 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    27601 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    27661 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    27721 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    27781 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    27841 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    27901 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    27961 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    28021 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    28081 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    28141 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    28201 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    28261 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    28321 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    28381 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    28441 agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    28501 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    28561 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    28621 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    28681 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    28741 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    28801 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    28861 cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata
    28921 tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    28981 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    29041 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    29101 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct
    29161 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    29221 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    29281 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    29341 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    29401 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    29461 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    29521 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    29581 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    29641 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    29701 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    29761 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    29821 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    29881 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    29941 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    30001 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    30061 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    30121 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    30181 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    30241 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    30301 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    30361 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    30421 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acagaactgt
    30481 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    30541 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    30601 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    30661 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    30721 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    30781 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    30841 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    30901 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    30961 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    31021 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    31081 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    31141 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaaataa
    31201 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    31261 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    31321 taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    31381 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    31441 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    31501 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    31561 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    31621 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    31681 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    31741 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    31801 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    31861 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    31921 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    31981 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    32041 ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta
    32101 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    32161 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    32221 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    32281 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    32341 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    32401 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag
    32461 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    32521 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    32581 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    32641 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    32701 ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat
    32761 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    32821 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    32881 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    32941 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    33001 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    33061 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    33121 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    33181 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    33241 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    33301 ttgtactgat ttgttgagga aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    33361 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    33421 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    33481 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    33541 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    33601 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    33661 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    33721 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    33781 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    33841 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
    33901 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
    33961 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
    34021 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
    34081 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
    34141 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
    34201 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
    34261 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
    34321 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
    34381 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
    34441 taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct
    34501 aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc
    34561 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
    34621 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
    34681 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
    34741 aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt
    34801 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
    34861 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
    34921 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
    34981 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
    35041 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
    35101 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
    35161 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
    35221 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
    35281 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
    35341 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
    35401 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
    35461 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
    35521 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
    35581 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
    35641 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
    35701 aataaccctt aaagcttcac gtggaatagt gaacgtctgc ttttcactgg acatattaca
    35761 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
    35821 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
    35881 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
    35941 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
    36001 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcct tgattgattc
    36061 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
    36121 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
    36181 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
    36241 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
    36301 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
    36361 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
    36421 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
    36481 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
    36541 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
    36601 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc
    36661 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
    36721 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
    36781 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
    36841 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
    36901 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
    36961 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
    37021 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
    37081 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
    37141 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
    37201 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
    37261 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
    37321 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
    37381 attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa
    37441 cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa
    37501 tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc tgatttcaca
    37561 acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga acagaccata
    37621 catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg gatagaagat
    37681 aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa
    37741 gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat
    37801 caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa ctgatagtaa
    37861 tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg tgatacctga
    37921 acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag gagaagtacc
    37981 aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc ctacacgagg
    38041 catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag ttcctgctgt
    38101 atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa gagaattgat
    38161 gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg ctgcggcaac
    38221 aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg tagctgccat
    38281 cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta cgactaatcc
    38341 aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta atgtagattc
    38401 attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata gaccgatacc
    38461 gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt cccgcagctt
    38521 gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta atgctgtttc
    38581 ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga tcccaagagc
    38641 aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc cgatcgcact
    38701 ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac ctaatacagt
    38761 tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg ccagtacctc
    38821 acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt tttccttcat
    38881 cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc gatttattaa
    38941 taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa aattcggatt
    39001 ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt tcatgaacat
    39061 tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat tctagtctca
    39121 aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg atgaattctt
    39181 ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc atttttcccc
    39241 agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc tcactatgtt
    39301 ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact gtttttcctc
    39361 ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc ttcagggtca
    39421 gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc ttccatgttc
    39481 tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg tcctaatacc
    39541 tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa ttcttttgag
    39601 atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga aatttctgca
    39661 ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata aagcatcaca
    39721 cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc ctgtctataa
    39781 actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa
    39841 taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa aaaaaaacgg
    39901 caatgaatgc cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg
    39961 gttagtgtgt gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct
    40021 tcgattgcct gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc
    40081 gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt cacgccagtt gagacagcga
    40141 cagatcgctc cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa
    40201 tctgcaccta aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct
    40261 gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta
    40321 tagatatcta caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt
    40381 aaagcttcaa tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc
    40441 cactgagcaa tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct
    40501 tttgctgcct cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta
    40561 tcgataccat ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg
    40621 gtatccacta ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga
    40681 atagatagtc tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa
    40741 atcctttttt acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa
    40801 tggctgtttt ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga
    40861 taccttgatg tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag
    40921 ccatttcctc agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat
    40981 cgatactcac taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta
    41041 gtgcataagc ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg
    41101 tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt
    41161 ttcgaatttc gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt
    41221 tgacaaaatt ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt
    41281 gttttgcttc ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc
    41341 gaatgatgaa aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt
    41401 tattttcttt atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc
    41461 gggcagtcgt tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag
    41521 gattttcgca aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga
    41581 attcgatatt gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt
    41641 cagccatttc atcccacctt tattttgttt gtaagactag ttcacctctt tttgttgcaa
    41701 atgcaactat tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct
    41761 tttcttcttt tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag
    41821 taactgtttc ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa
    41881 tcccatgttg gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca
    41941 agcaaaaaca agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc
    42001 aaaaactgct cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt
    42061 acgcacattt tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc
    42121 tttcgtccgt ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac
    42181 atctactttt ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa
    42241 agtttctttt cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg
    42301 ctctttatca aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct
    42361 tttgtattga gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact
    42421 atgtaatcaa gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa
    42481 taaataaaag aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg
    42541 aataaatggc gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg
    42601 acgagctatt cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct
    42661 ccccactaaa cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac
    42721 atgagaagct gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg
    42781 acctctcggt aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac
    42841 ttattgtcat tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta
    42901 tagtctgtta catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg
    42961 gcgatgatct ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc
    43021 aatacgtcac catagtgata atgatccatg atatagctca aatgacgatc tgattcacga
    43081 tcacgtttgt accgacgttg ccataacact tcaaagtctg ctgttaggcg aatcgtgatc
    43141 acctgatatc cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa
    43201 ggatactcgg acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa
    43261 tagaaaggat aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg
    43321 atggattctg catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat
    43381 cgttcccgta gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga
    43441 atgaaatact ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa
    43501 cagtccaatc ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg
    43561 gcgaagagac ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt
    43621 gactaaaagc tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata
    43681 atcttcttct ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc
    43741 gatttctttc agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt
    43801 accgatagca atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc
    43861 cggtcttgta tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat
    43921 tgttccaagt tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt
    43981 tgtcttcagg atattttctt tgaaaggcag atgggaccca tcatacatga cagaagtata
    44041 tccgctgtcg attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac
    44101 tgcatcaacc atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc
    44161 gatatattta gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc
    44221 tgcgcctagc atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa
    44281 attcaactct tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca
    44341 ccttatcttt ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat
    44401 ccattttgcc catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac
    44461 gcttctggtt atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt
    44521 ccaaactctt ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa
    44581 gtccaacttg ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt
    44641 gtttcaattg tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt
    44701 cttcttttgc cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca
    44761 ttttccctac tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga
    44821 gtgctgcggc tactgtatct acatcggttg ctgtagcatt gttgaatccc atcgaattaa
    44881 agatcgttac tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa
    44941 tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa
    45001 agaaattggt tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca
    45061 tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat
    45121 aaggaaagaa aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta
    45181 tccctcgaaa tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat
    45241 acacacccac tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc
    45301 ctgataattc agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga
    45361 ccatcacgac catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact
    45421 tgatatcttc gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg
    45481 cttcaaataa gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga
    45541 ttttacgaat gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa
    45601 aaccaccgac caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg
    45661 tcatcgttgc catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga
    45721 aacgagccaa aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac
    45781 ctaaacccaa atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt
    45841 gcatatgaaa tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc
    45901 tcgaaccggc actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt
    45961 gtcctgctga tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg
    46021 atgcctgagt caaaatattt tcgatgataa aattgataac gcccatgttt ttaccctctt
    46081 ttctttttgt atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat
    46141 ttcatttttt gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa
    46201 cttttcgaac tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac
    46261 tgcggaagaa atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc
    46321 ctcagcagac atctgtgctg caaaactact tcctaaacct gcaccgcata cgaataaaat
    46381 gttcaatttg ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa
    46441 aagcagttgt ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc
    46501 gttgttgatc aattcaatta gttctttcat gatgttcaga tgcgagtagg agtctactgc
    46561 tgccaaacaa aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg
    46621 ctcaatcgta gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc
    46681 tagtgccatg tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat
    46741 cgcttcgaca tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc
    46801 aatcgattcc tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat
    46861 atcttttaac ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa
    46921 tttttctaac ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc
    46981 tattatttga gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc
    47041 ttcatttctt ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc
    47101 gacaactaaa agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac
    47161 atccaattta tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg
    47221 aggaaacttt tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca
    47281 gacaacgact gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga
    47341 aaagtggatg acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc
    47401 gatcgtagga acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt
    47461 ttctttcaat ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat
    47521 gtgcttatag agattttcga ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc
    47581 gttctctaca aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga
    47641 attggagata tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac
    47701 ttgcgggaca ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct
    47761 actgtctttt atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat
    47821 tcgctggatc cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg
    47881 tttcaacaac ttattggaat agatctcctg tatttttttg atttccacca aaggaaaata
    47941 atggagaaag attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt
    48001 cattcccaga ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc
    48061 tttataaacc agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt
    48121 tgctcgtaac cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc
    48181 ttttttgttt agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat
    48241 acgttcgcgc cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc
    48301 ttgcttttcc ttttccagct tcaacaaaaa acctttgttg cgaattgtac ggatctccgg
    48361 aagcttttta ctgctcaacc agatattcgc ttcaagaact tcctgacgga tcgtccgctg
    48421 actgacattg aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat
    48481 tctgtgaagt atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt
    48541 atatggttcc gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag
    48601 taagcaaagc aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat
    48661 acgcctatta ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt
    48721 cctgcatcaa gtaataagaa caactaaact aaaatagtgc ttcctatttt tcgtttggtt
    48781 gttcttatta tcgcagatgc aagcttttaa acaccattta gtggagaggt tgtgacagag
    48841 atgttcagcc ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa
    48901 tcgtgcctta ttgccgatag gctatctctg gcacaccgtt tattcagaaa aaggagctgc
    48961 gacaaggctt ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggc
    49021 tgaataaaat cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga
    49081 gaaaaatact gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact
    49141 gaataatcac agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga
    49201 taatttccta cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg
    49261 tttttttgca gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct
    49321 ttttcaggag tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc
    49381 agaaactgtg ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgctttc
    49441 agccttttct tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac
    49501 aaaacagcta atgcaagaaa tcccgtttgc tcataactag aaggacctgt acctggacca
    49561 taatacccat aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa
    49621 cgaccaccaa atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca
    49681 cccgcgacag cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg
    49741 attgctaaat aattgatttt tttcattgtc ttcctcgtta tcttttatta caaaaaacta
    49801 tttttatgat agccttgcta aaaggaagag tcaataacca gatgcataaa ctgttttttt
    49861 agcaattttg tcttatttag tcattgagta acatcggaca aatgtatgtg tcggcagtcc
    49921 ataattcctc tatcttcagc atgaacaagc ttgcagacaa gagatctgcg cttcctccag
    49981 gagagatttg gttttcctga caaaagtgca acagtgctgt aaatggacga tttggtaaag
    50041 cactttgact agcagcatga gcaagttttt gcatttccac taacgttttc tgatcactgc
    50101 gatgcaatac gcatgtgtca tctagcgaac tcatcaaaac taacaacata tgcatttgtt
    50161 tctgtacata tgaatcatta tctagctcct tagctgcttg aattgcaagc ctgatatgcg
    50221 gataccctaa tatcgcttct tcatatgcac catttacgcc atatttttgc ttcaccctat
    50281 gaccatgagt ggttttttta tgacgatatt ttgtatcagg aaaagcagca agcttgctga
    50341 catcatcaag tattttcgat aaagaaaaag gctgttccat actgattttt tgtgcaatga
    50401 cactgatcaa tagccccatt gcccaaatcg ctcctttatg ggtattgatg ccatttgtag
    50461 caatcagcat tgccttttcc gcttttcgcc caatctcagc aattctttct cttaactcct
    50521 gatcaatagc atgatgccat gctatttgag ccatttcttc aaaaaaagga gttagcgtct
    50581 tagcagactt aagaaataaa gcatagtcca tatcggaatg tgcacccgca tccagtgcat
    50641 ccactaatcc aggttttgga cttaatgtta cttctgcgat caatgcttcc tctaccaacg
    50701 cagcgagttg ctgagtcact gctttttttc gttcgtcatt tatcatgaca ctcactcctt
    50761 cttcctacta ttacttgctg aaaccttttt tgatagctac ccaaatgata tgaagtaacc
    50821 agcttaaagc aaatgcacaa cttgccgtca ttagataagc tacaagaatc atgactcctg
    50881 aaggtaactg cagccaacta tctttaaaca ataataacag tacttggaaa acaaatacat
    50941 tggctaaata cgtacgataa gcataaacag acaaccattt gaacaatggc aattttggcg
    51001 aatcgtttac aatcattttg gaagcgccta tgaagacagc aaagatgatg actaaactgt
    51061 ataaactttg gatcgtattc aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg
    51121 cgagcaatga acggacagaa aagaacgcga taactaaggc aacaggtaag aaaagataac
    51181 gtactctgtc caaatattga tagacttttt cgtgataaac aaatgcggct accccataaa
    51241 tcatgaaact agcaacaaac cgatctaata agtaccagct gtcatgataa ggcccagtca
    51301 atacttgtgt ttgatagaac agataccatg cgacatataa aatcgttgaa ccaatcaata
    51361 ctggccatac atatttttta ttagcaaata ctttgtaacc gagccaaaca aaaaacggca
    51421 tgaacaattg gatctgcagc atcataacgg tataccataa atgaggcgct ccatcacctg
    51481 tgacatattt cagtaaaaag cttgcagtat ccgtatacgg agtttgttgt tggactgctg
    51541 gaaatagcca aagataagcc gtcgtccata gaagatacgg aacgactagt tcaaaaaact
    51601 tttctttcaa aaattcccaa taagacaaat gctgacttgt ttttaccata ttataaacaa
    51661 ttgcaaaaat aaacattggt gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag
    51721 ctgtatttgt tgcgctgtgg ctgtttgcca aagcaaagct caatatcgtt tgcatcataa
    51781 cagccgttac agctgatact ttctggaagt cacttacgtt gattttgatt tttttgtttt
    51841 caaacatctc tttctctctc tttttcttta ttaccaaagt tctttggtta atttaggtcc
    51901 ttcgactgtt ttcaatagaa tttttttctc tggctctctc aaatactctt ttagtgaaaa
    51961 accgccttgc cgagtcacta cttgggtatc gaattgctca aaaaaagcag gatgcgattg
    52021 aattacctga attggcaatt caatcggtga atttgcgtac agcagcaaat caaaatcact
    52081 ggtttctttt actgccggaa tcccagtcga taattcaaat cctaagctgc cgccgattcc
    52141 ccatttacat cctgataata tgtttctagc agcatgaaat tgctgataaa caggataatc
    52201 catgattttc tcttgtctat acgattcttg ttcgatgatg tcccacggat gaatgacttg
    52261 tttgacggca tgttttgaga tttctgttga ccatctctga ttgcgggcag gtccacgtaa
    52321 accaatcggt agtatttctg cgtgatccgt cgacgcacga cgaactgttc cgaacatctt
    52381 ttcagttaga acgacccatt caggcaaggt gaacccttct atttcatgtt tatcaaaaag
    52441 gacaatatca tgtgcttgaa tcatttttta tgcatcccat tcttcgtcca ttttggcacg
    52501 gactttattt gtttcgacac gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt
    52561 cagcatcgtg tcatttgtat tgcggacatc ttgaatggct tcattgattg cttcgatgac
    52621 tttgctgaca ttttgttctg aagcagcttg gtcggtgata tcttctagga aatgatagat
    52681 cgctcctagt tttgtataat tattgatatc gtaagcgata gctggaactt tttcagcagc
    52741 tgcttcgatt tcagcaaccg aacgttttgt cacacgtgca gcactttctt tagacatcgc
    52801 ttggacggta atttctttat tgtttaaagc aatcaagcgg cttgattgta atccatgtgc
    52861 taaaaatgca cctgaaatag catttcctgc aatcaatcca ataactggat gtccagcttg
    52921 acggagtttt gcataagcag cagcactatt agccaaagcc acatggatac cgatcaattc
    52981 ttcgttatag ccgtatgctt ggcttggtac atcgatcaca acgatgattg gccgtttcac
    53041 ttttttgtct ttatcttctt cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc
    53101 aactaaacca acttcaccgt ttcgtacacg gtacatcggg tttttaggat caggtacgat
    53161 tgatgtcacg atggcttctt ctatgaattt acttgattta ccataataca cagtactaat
    53221 agaagaagtt ggatttttca tgccagttag tgccgcaaac caatcgtaac caacacttgc
    53281 tgcagctggt tcatttcctt ctgctacttc tggtaagtca attgattttg cttgatggtt
    53341 agcaattgct tcattgtaat cgtcaatggc cattggtttt gaaagatcta aagaatcaag
    53401 taatgataaa taaaatgctg agctttcact gcgatgcgtg tcttttcgtt cgtccattgc
    53461 tgcgatgaca gcagctttga ttgcttctac actatcagtc actaattcat caatgatttt
    53521 tgattctacc cgttgacgag atcctaatgt attccaaatc aagtctttgt cactagaatc
    53581 aaattctcga acgcctgctt cttgttcaat aacttctggt ccattcaaac cgactctcgc
    53641 tttttttgtt gcgattaaat aactcattaa tgctgaagta atcgacatac cgccaaatga
    53701 tcctacacgt cctggaatca aaccgatgac tggtacatat tgttttagag caatgattac
    53761 atttccgatt tctgaaatag ataacagtcc gtagtttgct tcttgcaaac gaacaccacc
    53821 tgtatctagt acaatgactg gaaagatctc atttcctttt ttattttctt ctaacacatg
    53881 ttcaagtgca gcaacgattt ttgcaccgga aacttcaccg ataccgccac cttggaattt
    53941 tccttccata gcgattacta ctgcttgttt gccattgatc gtgccacggc tgacaacgat
    54001 cccgtcatca ctttcaggga cgattccttg tgctggtaaa cttggcgcga tgatttgatc
    54061 gaatggatca atcaattcac gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt
    54121 taattctaca aaactatttt tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt
    54181 acacaactcc tggtgtagca ccgaagtcat tgatcacgat atctgctgaa attggataac
    54241 gatcaaaaaa tctcgttaat acattgttcc atacttcgcc gaagccatcg cttcctgtac
    54301 aaatttttac agtagattct gttttttctg ttggaaacat caagatttcc aaatctcctg
    54361 aaccgaccac tcctacatgg attttcttat tgatcggctg tttcgttgga tatttataag
    54421 ctaattgttc catggatctc tccccttttt gtaaaacaga gcatacacgt tctgcttatt
    54481 cgtctttctt cttccatctg ggtaaatggc gacaaaaggt ttttctcgcc aattttatga
    54541 ccaagttctg aatttcattg gtggttcata caggccatct gaccattcga tcaaatcatc
    54601 gatggtttgt gcagctaata atgaacgttt cgcttcacta cgtttgatgc ctagatcttc
    54661 tggtaatttc acgatgccgg ctgcacgcaa gctatctagt gattctttcg tgctagtcat
    54721 tccaagaggt gtaacaccgc caattgctgc aattgctgct tgtcgttctt cttttgtttt
    54781 tgctttgtac aaataagcaa ttccttcttc tgtcacgata tgtgtagtat cttcgctata
    54841 aatcatcact ggtgcattgg ctaagcctgc ttgtttttga acagctagtg catctaattc
    54901 ttcgacaaag actggttttt tatttgaacc atatgtttca accatttgaa caactaattt
    54961 ttggcctttc cctaatggat catcattgtc gcgaagtgat tgccatgctg gtgtagaatg
    55021 acgacgaccg ccaggattat gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc
    55081 ttttgttacg gtagaagagt ttccttcgta atctaattgc aaggttgaac cgataaacat
    55141 atcgatggca tattgtcctg cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc
    55201 tttaccgaca aaaaagacat ctgggcgagc agcaatatat ttttccatac caacttcgcc
    55261 accaaaacta tggatgcttt caacccaacc tgattcgatg gctggaatca tcgttggatg
    55321 tgggttcaat acccagttct tcgcgatttt cccttttaac cctagtgatt caccataagt
    55381 tggcagtaac aattcgatag ctgctgtatt gaatccaata ccgtgattca aagattgtac
    55441 gccgtgttct gcgtagattc ctttgattgc catcattgcc attagaattt gcaattctgt
    55501 gatgttttga ggatctcgtg tgaataaagc ttctaattga taaggtttat ctgctttcac
    55561 gacaacgtct acccagtcac cagggatatc tacacgtggt aaagaatcga caacttcgtt
    55621 aacttgaacg atcacgatac catctttgaa agctgcagct tcgatgattg ttggtgtttc
    55681 ttccgtattg aaacctgtgt acaagttgcc gtctttatct gctttatctg cagctactaa
    55741 aacaacattt ggaatcaaat cgatgaataa gcgtccataa agttccaaat aagtatggat
    55801 atcacctaat ttcattgtgc cgtcttcaat catttgggcg acacgcaagc tttgtgcacc
    55861 agcataagag aaatcgatct tgcttgcgat tcctttttca aaaatattca aatgttctgg
    55921 tcgtgagata ctagacatga tcaaatgcag atcattcact ttttctgcat ctactttttc
    55981 aagactttct gaaagaaaac ttgcttgttt ttgattgtcg ccttctaata caactttgtc
    56041 gccgggagca atcagttttt ccattacttc tacgattttt tcagtttcta catacttgcc
    56101 attgagcaag ttgctgattt tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca
    56161 gttgcggttt ggtttttcca taattagtca tctccatttt tgatatagaa acgtggcgtc
    56221 atcaatacca tgttggtcta gattgatcgt acggatccct ttatctacac caaattttgt
    56281 atgaatcagc tttgtcaaag tattgccggg gggaaattca atcgtcagat ccattcctaa
    56341 ttcttttgcc aagtccatca tttggttcca ctgcacaggg aaagctaggt tttccaacaa
    56401 atctttacga atctcttctg cgtcctgagt ggctcttcca tccgtatttt ttatatacaa
    56461 acatctagca tcagatagat ggacagactg catatacggc ttgaatttct tgaccgtatc
    56521 attcatcaag agacagtgag atggcacagg gactttcagc agcttcgctg tttgcgcttg
    56581 gtgccgtttg gcttctgcca acgtaacgga taaaccttcg atactcccag ataacgtatg
    56641 ttgcatcgga caattttcat ttgacagata aactggatgg tcaggatcaa atgtttttgc
    56701 tacaatttcc tcagcttcgc ttctcgtcac acctactact acgcccattg cataaccgct
    56761 tggataagct tctttcatta agactgcgcg gtgatagact agttccgctg cattctcaaa
    56821 tctcaaagat cgacaagcaa cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat
    56881 atcaggctga ataccgtttt tttccatttc atctaaataa aatactgctt ttgtcagtaa
    56941 tgctagctgg ataaaaaccg tatcttgata gttttcttct ttatcaacta accgataccc
    57001 cgtcacattt tctaaacgtt ccatgtacat ttcagggata tccgctaaca tccctaactt
    57061 ctgactgcct tgtccaggaa ataagaaacc tgtactcatg agcctcgcct cctcagcact
    57121 tactttttcc actgctatct tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa
    57181 aaaaccataa caaaaaggta tggatgggat ggataattat gaaattgctt catctgcagt
    57241 attttaaaaa agtattcgaa acaaaaaatg ttacccaagc agccaaagaa ttatttattt
    57301 ctcaacctgc cttgagccgt gcgatcaaac atttggaaaa cgaattaggt gtccctttgt
    57361 tttaccatag cggcagaaac attgagacga ccgtttatgc agaagaattt tatccatatg
    57421 cagtcaagac attagacaca ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga
    57481 agatcgttac ttccgtcata ttgtatttag aggtagcctc tgtttctatt ccgaacttgg
    57541 tccgaatttt tatggaaaag catcctgata tccaattaac gatcatgcaa catcatttac
    57601 cagatgatat taccgatcct gtactgtaca tcacttcgga gatgaagcct ggcatgacca
    57661 atattcctat catgaaagaa cctgtttatg tcgccattcc taagaaacat cctcttgcta
    57721 aaaaagaaca tctgcaatta gaagatatcc aacgcacccc catcttgatg ctgtcaaaga
    57781 gcaacgcctt ccgtcataca ctggatgcag cacttgaagc gaaaagtatc gaattgacga
    57841 tcagttcgac gacagatgat cctgcgacat tacgttctat tgtcaagcaa ggattagctg
    57901 ttagcttttt cccaaaagtt tcttggtctt acgataaaaa tgatcctttc gtcttgcggg
    57961 aaatcaaaga tttgcccttg acacgaacaa tttatttatc ttctagcttc acagaagatc
    58021 atcctttgat caaaatgatt gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa
    58081 aaaagagctg ggacaaaatt cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa
    58141 gtaatttctt agaaaataag ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat
    58201 tccttcttat ttcttgaagc taaacatttc tgtcacaacc tcttctctat tgtcctagac
    58261 attcattgcg ttgatttaaa acttccatca atcaaatctg ctagttcatc tagcttttct
    58321 attttacgta gatcaataga gatccaatgt tctttattca tgtgataggc tggaaaatac
    58381 ccctcttttc ctcgcaaaat tccgccaagt tcaggatcga ccttcaaatc aagaatattg
    58441 atgatcctat ggtcacttgc atcatcatag attttttctt ctggaacaga catcaggacc
    58501 ccaaaccatt ttttgctttt ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa
    58561 atatattctg gagacaaatt gtatgtttca ttgatgtgat cgattagttc ttttttcgtt
    58621 agcatctgct tccatctctt tcttattgat tcgctttatt caaagatacc acgtaagaat
    58681 agaaaaatga atacaagtag ttgctaatca aatacttcga tatttttctt ttttatcaac
    58741 tcttaaatgt tcaatccgcc tgtgttggtc ttgctaccat ctactattaa aatgtttatt
    58801 attgtttgcc cacctatttt ttgattttag tggtatcatt tttcaagttc atgatgaaat
    58861 tcttatcatc agtaaatgct ccagaaaaat aaacaatgat tgaaaaatca gttgaaactt
    58921 gaccaccgtt tattttctcc gtattcttag aatagaaggg agtctgattg atgtattcac
    58981 taatgaaaaa aatcattacc gaaaaagata ttcgacgtca tgtttcttta gtagaacaat
    59041 tgctgaatca tacaaagata accgtaaatg aattagctga aatcataggg acaaccgaac
    59101 gaacgatttt ttctgacctt caatctattc gctctcagct gcccgaaggc tgggacatca
    59161 tcagcgatca agcaggaatc agtttgcaaa accagcaaaa tttattaaca aatgaccttt
    59221 gggagatttt tttcaaacaa tcagtcagtg tcgaactgct gaaaaatctg ctttttacaa
    59281 aaaaagttgc tgtccctgat tttctagccg accatgggct ttcttatggg acattgaaaa
    59341 gacatgtgac taaaatcaat cagcgtttag caagttacga cttgcagatc gatttgacga
    59401 aatatacagc ttgtattctt ggaaaagagc gtgcgattcg tactttttat catcgattgc
    59461 tgattccttt cactcataac aactattttt tcgaggatta ttcgatccat gaatcccatt
    59521 attttcaatt tttaagaaac ctcagtcaaa ctgaactagc ggttgaaaca gaagagattt
    59581 tcggaacatg ttggttcttt atcaatacga tccgtataaa agcgaattgc cgattagatt
    59641 cttctatcca taccaacagc acactctcct ccctgtatga ctctgcattg aaaaaattgt
    59701 atttaacgga aggtatttat cttaaagaca ctgagctttc ttttgcttct ttctgctttc
    59761 tggaaagttg gaattacaac aataactatg gacaagaaac cgctcgctgc ctgcatcatt
    59821 ctccattttt agaggtcctc gaaacgtttg tagaggaact agcttcagag ctgtctcttg
    59881 atcaactaaa aaaaacatct ctgactgata acttggctct cctcatatta aaatatcatg
    59941 aatcgccgat attgattgaa gcattggacc gtcaatacca tcgtttttta gaaacatatg
    60001 atcagcaata tgcacacttg tatcaacaga aagagaattt actgaacgat ctaaaaaaag
    60061 aaatagaaat caatgaacca aattactttc tccaactctt gtctctactg atccaacaaa
    60121 cgattttttc tatcaaaccg aatttgttca atgtctattt tattttccaa ggagaacctt
    60181 catggaaggc gttcttgcag caggaattaa aagattactt aggaaaacgt gtctgcttct
    60241 tacctatcga actaaccgat ctttccacta tttcttttga aaaaacagat atcttgattt
    60301 ccaatttccc tctagattct ctagatatcc ctattgtata catttcttct attccaacga
    60361 aaaatgagct caatcgtttg actgaactca ctttcaaaac ttttttatag taacggctag
    60421 cgactgtatt ctctagctgt tttttctttt ttgcgaaaca gtttgttcat gatccctttt
    60481 tctatttcta caactaagaa tagaagaatc acatttgctc caatcaataa ctgttgtgtc
    60541 atcgttaaac ttactgtacc gatcagctgc tggccgactg gtagaaaaat aaccatccct
    60601 tgcaataaag caaggatacc taatgaaaga aaaagcgctt tgttttgcaa aagaccttgg
    60661 ttcaatgacg gcttggacaa ctctctgcaa ttgatcatgt aaacagcttg agctaagacg
    60721 atattttgca gaagaatggt ttgctgtaag ctttgccctt caaactgcat agccaataaa
    60781 taggcaggaa ccatgatcag tagggagaca tagatgatcc gaaacaaatg ataaccagat
    60841 aggattcctt gctttggatc tcttggtggg cgcttcatga tttctggatc tggcgactcg
    60901 aaccctagtg cataagataa agtgattgtc gttaccatat tgacccataa aatctgtact
    60961 ggggacaaag gtagcgggcg attcagcata agtgcccaaa tcaagatcaa tccttgagct
    61021 aatgcagttg gcaagaagaa attgatggtt ttttgcaaat tatccaaaat ccgccggccc
    61081 tcttttacgg cttttgcaat cgtatggaaa ttatcatctc ctaaaaccat atcagctgcc
    61141 tgtttgctta cttcactacc tttgattccc atagcaattc ctacatcgga tcttttcaaa
    61201 gcaggtgcat catttacgcc gtctccggtc attccgacga tttctccatt tttctgcaaa
    61261 gctttaacaa ttcgcagttt atgttctggt gtcgttcgag caaagacatc gactattggc
    61321 acttgctgaa tcaactcttc atcagacata agatccaaat cgatcccttc taaaactttg
    61381 gcagtatgtt ttaagccaat ttgttccgca atcgcttttg ctgtgtcttt gtgatcccct
    61441 gtaatcattt tcactgagat ccctgcttgt tggcattcct ttacagcttt gactgcactt
    61501 tcttttggcg gatcgatcaa tccggcgatc cctgcaaaag ttaattgggt taaagtctca
    61561 tgcgtgagtt cctgtttcga atctactttt ttataagcaa atcctaaaac acgctgacct
    61621 tttctcgcaa attctgcagc tgtctgactc catgcttctt gttctgcaga agaaagagtt
    61681 gatttagcaa ataatacttc tggtgcacct ttgacgtaaa tcaccgctcc tttttcctcc
    61741 gcatgcatcg ttgccatata tttgtattca gaactgaacg gaatctttgc ttctattttt
    61801 ttcaaaggta gattggctgt ttctgtgtgt cgaatcagcg caacttctgt cggattgcca
    61861 cttaaatttt ctgttttctg ttcttctttt gtttttaatt cctgacagtt tttcataatg
    61921 gacaacacgc aggcttcttc ttttgcagct acatccacaa ctgtcatttc attttttgtc
    61981 aatgttcctg tcttatctga acagatcact gtcatagaac ctaatgtttc taccgaagga
    62041 agtcctttga tgatcgcctt ttcttttgcc atttcatgta ctccaacgga tagaatcatg
    62101 gtcaaaacag ctggcaatcc ttcgggtacc attgacacca ccaaagcaat cgtagccgaa
    62161 agaagaatat gccaatccat accatatcga aatgtcgtaa aaataactaa gaataggctc
    62221 aaacaaagaa tgccttgaaa aatttgtttg tttaatcggt tgattttttt aatcagaggc
    62281 gtcgtttgtt gttcgaccga ttgcaatgct tgattgattt taccgatttc tgtcgtgttt
    62341 cctgtttcta caacgacacc gactgcagaa ccagattgta caagcgttcc tgaaaaaccc
    62401 atatttttct ggtcgccgat cgattcttct tctgtcaaaa cggcatgact tttttcagat
    62461 gcttctgatt ctcccgtcaa aatcgattct tccactaata aatcatgtgc ttcgattaat
    62521 cggatatctg ctggaacgac atctccggca cgtaagacaa tgacatctcc ttttaccagt
    62581 tctttcgcag ggattttttc ttgcatcccg tttgatagta ctatcgcttc ttgtcccatc
    62641 agctgcttca aaccattcaa tgattcttcc gcttttcttt cttgccagta actaacgagt
    62701 ccattgacga tcactacgaa taaaataata cttccttcaa tatattcact agaagcaaat
    62761 ttcaaaaaag aggcaatgat caacacgatc atcagcagat cagtaaaatg ctttgctatt
    62821 tttttccatc gttttgtctg ctgccttgct tttatttcat tatatccttg atcagtcagg
    62881 cgtttctgtc gttcttcttg cgacaagcct tctgttgatg tttgatagtt tttcaataca
    62941 tcttctaccg tcaatctgta ccagttcatc ctatcccctc ctgtttaatt gatactgtta
    63001 ttatacgcag ggaagttttt tcaggcgtat cgattatttc aggggggtct gaaataattc
    63061 gttttgcaaa atgtatctgg gcctaactga tatttctatt gcttattctt ttatatacaa
    63121 aatttaagaa tacaaaccag ataaccaata aaaaaggaat aaagcgtgtc gtgctttcga
    63181 ataacaagct gaaagcaatt gaagtaaaaa acaccaacaa agcaagatct ttatatcttt
    63241 gtgtagtgtt ccgtttcttt tctttaataa attgaacatg tgcacacaag atcgaagacc
    63301 acacaaatag aaaacaactc gttgtcatgc ctgccaaaag ctgaaaaaac aatcgactat
    63361 cagctacaaa aaaatgtaga caaagaccgg caaaaaataa cattcctgta attaaaatac
    63421 cgtgtaaagg tacgcctttc ttagaaagcg cacaaaacca atggggcaaa cttttttctt
    63481 ttgctgtttg atataaaatc ctgctagtac tgtagactgc actatttgct gaggacaaag
    63541 aagcaaccaa aatcattaga ttgacaattg ctgctccgcc ttggcaatgg atcacttgca
    63601 gcatagtcac taaaggactt tcttttaaat ctaaacaatg ccatggaaca acaaataaaa
    63661 tcgacaaaat cgttcctata tagaaaaaga gaatacgcca aggcagacag ttgattgctt
    63721 ttttcaaatt cttttgcgga tcacttgtct cgccaacggt taagccgatc atttcaattc
    63781 cagcaaaact gaataaaacc ataggaaatg agactaccat tccagcaaag ccaaacggta
    63841 gtaattcatc gatctgtcca aaattttctt tgattccgat agactgggta tgaaaatctg
    63901 agacactcaa tcctataccc aataagataa aaaggaaaat ccccaagatc ttgatgattg
    63961 agaaaaaaga ttccaattca ccaaaccact tgactgcaac aaggttgact gccattaaga
    64021 tcattagtgc gctacctccg cttatcaaag ctggtacatg tacaaaccag aaatggatat
    64081 acaatcctat tgctgttaat tccgcaagtc cagctccaag ccagcaaagc caataagtcc
    64141 aattgacaaa aaatgcccac tttttgccta aataatagtc aacgaaatca ctgatagagt
    64201 taaatgtact atttgctaaa aataaatcac ccatggcccg catcaaacca taaatagcca
    64261 aaccgcatat caaataaacg agaataacag ctggccctgc tgatgcaatt gcctttgctg
    64321 aacctaaaaa taatcctgtt ccaattgcgc cacctaatgc gattaactgg atatttcgat
    64381 tagttagagt ccgtttcatt gccggttttt ccatcaagtt cacctttttc tttttcaagc
    64441 agcatggcga cttcctgttc ccaagaaggt ttctttattt tttcaataca gagtggtact
    64501 acaaacaaaa tgacactgat ggctgtgact gcaattagat tgatccatgt tccagctttc
    64561 atcgtcagtc cgacagaaaa tccgattgcc gcaaataaaa caataacgac agtccaaact
    64621 ccaagattgc tctttttctc tttttttcca attctaaatg gtcgattcaa ctttgcatgt
    64681 gtataacgca atctcaaaac tgcgatcccc atgatgacat agacgataca gtagataaca
    64741 gtagttgaat ttacgagcat caaaaatgct tgattgacgc caggaatcag caaataaaaa
    64801 agtgcaaaga ttgagataat gacagactga gtcaaaataa cggatttgga tacgtcaaat
    64861 tgattcgtct tccaaaaatt gaattttggt ggatacgcac cttttctagc actttctgtc
    64921 actgtctttg ctggtcctga ggcccaagca gataattgga ctgctacacc aatgaagaca
    64981 agtaaactaa acagattcac aataagatgt ggcaaaccaa gaatttggca ataaatcgaa
    65041 atagattgcg cgatattatt caattccatt tgtccttttg gtacgacatt tgctacaact
    65101 aaagcattca aaatattgac gaaaaatata aaaattaacg cggcaaatac tccgcgaata
    65161 tacgttttaa ctggattttc caatcgagtg atatacacag aggacatttc gataccagta
    65221 aaaataaaca tgatcggcgc aaaataaaca aatgaggatg cagtcgtagt atctggtatt
    65281 aatttgtccc atgaaaaact gcctaatgtt ccggttggta cgatcccaac tttgatccac
    65341 gcagcaaacc ctaataagag catcatcgtc aaaggcacgt acaatcctaa ccaaacaccg
    65401 acttttccgc cgatttttgc catatcaaat ttcaaattca aaaaagtgat gatccaataa
    65461 acgactaaaa tgacaactaa tgtaaatttg ctgttcaaac caagcggcac attatcgata
    65521 atattaccaa acaacggagc gagtgctgaa gcgaccatca ccatcccagg aaacatttgg
    65581 acccataaca gccatgagac aacgaacccc catttttttc ctagagcatt cgatacccaa
    65641 agttctggac cacctttttg gggaaacatt ccagcaaatt caccagagat caatgagatt
    65701 ggaaaagcgt acaataatac tgcaaccagc atgtaaaaaa tcatcgtcca gctcgttgct
    65761 gctacatctg gaatatttcg tacacttgct acaagggcgc aggtcattcc gataaaggtc
    65821 atggtcgtga tttgctttga tgaaccagta tctttcatct ttattccttc tttcaatagc
    65881 gacatatgtt tttgttttct tttatttttg ttcaagaaca gaaatgacaa ggtttcatcg
    65941 attcagtaca aaacccagta tcaccgaaac atccattgaa cgatcttagt gaactgggtt
    66001 tgatacatga aacgaacgat ccaacttata gaacgtttta taaaaaagaa tatcttggcg
    66061 aggaatatat gaaaaacagg gggaaataca attgttttga tgaagcaaat aattgtctgt
    66121 tctccatatg tctcttgtta ttctttcaca caaaaatcag ttctattgga tgatttcgtt
    66181 tagtttcttt tccattgctt tggcgatttt ttttgtataa aaattgaaaa ttcgatcatc
    66241 attcaaataa ggagtcatga tagccgcacg tagcagagtg attttctgtt cttttttcca
    66301 ttcttctttc gttagaccca ttctttcaac aaaacgaatc ggagaatcac cgtaatcttc
    66361 ttgtgtaaat gtagtatgag aagtaataaa ccgttcacca taaacatcgc catccatata
    66421 agaagaccga tcgaacattt tttcatttaa ttcattgata gcgttcaaat ctgtacagcc
    66481 ttgttctttg aatacccaat tgaccatatt aaaatcagga tgattcaatg gatagacttc
    66541 gattgttttt cctttaacag taaaagtcag atgatcgaga aattctctga atctttgagc
    66601 tgcctcgatt gaagccccaa tcaattgacc atatccggtc acattcaatg gcaatacacg
    66661 atgtgctgtc cagactgcgg cggcggttgc tccagctttt gatccttcca aaatataagc
    66721 tcctaacata tctggagcct ttactgattt ttcaaacaca tatggcgcga aataagaaat
    66781 gatatttctc atatttttat gtttgattac gattcctcca gcagcataag ggacatagcc
    66841 catcttatga ggatctatcg tgacagaatc cgcttctgaa atagctctga acccttcata
    66901 tacctctttg tcaatcgtta cgcagtggtg gaaaacatga tgttcttcaa agaattcagc
    66961 taaggaagca tacggcacaa attctcctgc ttcgtttaga aacagtgaac gcgcatagcc
    67021 gccatatgcg gcatcaacat gtaaatagaa atagatccct tcatctttta acctttctct
    67081 taattggacg attttatcta cactgtctac ttgaccttct tctgtcgttc ctacgacagc
    67141 aaccacacct agaattggga ttttttgtcc agctaattca cgaattgttt tttccaaagc
    67201 attaatatcc attcgataat cctcttgaac tgggatagca accatctgat ctaagccgac
    67261 tccgcaaata tctaacgcct tcatccaaga atagtgtttt gtttgaggga ccagccattt
    67321 ccctaatctt tggatatttt ttccgctacg ggaagaagct gctttgacct catccatttc
    67381 ttcgtccgta aagctttcgg tcatttccaa aatttcttca acagataaat tcaaaagttc
    67441 ccactcagac atttttttca ctttttcagg cagcacttcc ttgacagcta aaggaatcga
    67501 tttgatgcaa cgggcgtacc ataatccttc aagattagca atagaaccat ctgctgcaat
    67561 gtgtccccat ccgtctgtca tattgaataa tgaagcaaaa tcctgtccta cttctgcttc
    67621 catttgcgaa gttgccatag aagattccaa tgcaacatta tttgggttcc acagcatcgc
    67681 atagttgtaa gctaataaag caggcatcaa tgtttcagca ttcatttgtc cccagtaacg
    67741 gcctgctgaa tgccatggga tcgatcctgc gcgtaatcgc tgactgactt catccaagac
    67801 ctctatcata tggtcgcggg tggctagata atctggagtg agcttatctc cttcgctaat
    67861 tgcaggcata tctgaaggaa tataattttc tctccatcca agatgttcat ctaccatctt
    67921 gttcaacaac attttataaa ctggtccatt ttcagctttg tcgcctataa agacggcctt
    67981 gatatccata tctttcattt ttcttccctc catattttgt tggtcaatgt cttgcagata
    68041 catgtgttta tctgtaacac ctcttcacta tagcgccaaa aatgagaatt ttttttttat
    68101 aacatcaaat ccacgtagat ttttacaaac aaaaataaat aaagtcggat ttttctgata
    68161 attcaacgca aaaaagcgaa caatttttca ataaatcagt cttctaaaat agataaccta
    68221 aaaaaagcga ac
//
LOCUS       DAYYAO010000002        68131 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000002 DAYYAO010000000
VERSION     DAYYAO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.002"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /locus_tag="ACHARI_000061"
     CDS             <1..420
                     /locus_tag="ACHARI_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8441147.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="ACHARI_000062"
     CDS             complement(504..1226)
                     /locus_tag="ACHARI_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8441148.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="ACHARI_000063"
     CDS             complement(1216..1953)
                     /locus_tag="ACHARI_000063"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441149.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="ACHARI_000064"
     CDS             complement(1965..2957)
                     /locus_tag="ACHARI_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8441150.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="ACHARI_000065"
     CDS             complement(2972..3715)
                     /locus_tag="ACHARI_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441151.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="ACHARI_000066"
     CDS             complement(3956..5278)
                     /locus_tag="ACHARI_000066"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8441152.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="ACHARI_000067"
     CDS             complement(5265..6230)
                     /locus_tag="ACHARI_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441153.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="ACHARI_000068"
     CDS             complement(6227..7765)
                     /locus_tag="ACHARI_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8441154.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="ACHARI_000069"
     CDS             complement(8181..8468)
                     /locus_tag="ACHARI_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441155.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="ACHARI_000070"
     CDS             8602..9270
                     /locus_tag="ACHARI_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8441156.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="ACHARI_000071"
     CDS             complement(9561..10301)
                     /locus_tag="ACHARI_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441157.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="ACHARI_000072"
     CDS             complement(10431..11378)
                     /locus_tag="ACHARI_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8441158.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="ACHARI_000073"
     CDS             complement(11507..12271)
                     /locus_tag="ACHARI_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8441159.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="ACHARI_000074"
     CDS             complement(12275..12664)
                     /locus_tag="ACHARI_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441160.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="ACHARI_000075"
     CDS             12799..13341
                     /locus_tag="ACHARI_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8441161.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="ACHARI_000076"
     CDS             13489..14121
                     /locus_tag="ACHARI_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441162.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="ACHARI_000077"
     CDS             complement(14255..14380)
                     /locus_tag="ACHARI_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8441163.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="ACHARI_000078"
     CDS             complement(14514..15407)
                     /locus_tag="ACHARI_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8441164.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="ACHARI_000079"
     CDS             complement(15589..16515)
                     /locus_tag="ACHARI_000079"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8441165.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACHARI_000080"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACHARI_000080"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8441166.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACHARI_000081"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACHARI_000081"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8441167.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACHARI_000082"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACHARI_000082"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8441168.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACHARI_000083"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACHARI_000083"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8441169.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="ACHARI_000084"
     CDS             complement(24786..25220)
                     /locus_tag="ACHARI_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8441170.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="ACHARI_000085"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="ACHARI_000085"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8441171.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="ACHARI_000086"
     CDS             complement(26117..26992)
                     /locus_tag="ACHARI_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8441172.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACHARI_000087"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACHARI_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8441173.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACHARI_000088"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACHARI_000088"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8441174.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACHARI_000089"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACHARI_000089"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8441175.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACHARI_000090"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACHARI_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8441176.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACHARI_000091"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACHARI_000091"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8441177.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACHARI_000092"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACHARI_000092"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8441178.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACHARI_000093"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACHARI_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8441179.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="ACHARI_000094"
     CDS             complement(35443..36210)
                     /locus_tag="ACHARI_000094"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8441180.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACHARI_000095"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACHARI_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8441181.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="ACHARI_000096"
     CDS             37323..37721
                     /locus_tag="ACHARI_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8441182.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37958..38500
                     /locus_tag="ACHARI_000097"
     CDS             37958..38500
                     /locus_tag="ACHARI_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8441183.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="ACHARI_000098"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="ACHARI_000098"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8441184.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="ACHARI_000099"
     CDS             complement(39286..39513)
                     /locus_tag="ACHARI_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8441185.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="ACHARI_000100"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="ACHARI_000100"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8441186.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="ACHARI_000101"
     CDS             complement(40048..40761)
                     /locus_tag="ACHARI_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8441187.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="ACHARI_000102"
     CDS             complement(40764..41660)
                     /locus_tag="ACHARI_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8441188.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="ACHARI_000103"
     CDS             complement(41818..42660)
                     /locus_tag="ACHARI_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8441189.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="ACHARI_000104"
     CDS             42792..43445
                     /locus_tag="ACHARI_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8441190.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="ACHARI_000105"
     CDS             complement(43508..44035)
                     /locus_tag="ACHARI_000105"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8441191.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="ACHARI_000106"
     CDS             complement(44055..45002)
                     /locus_tag="ACHARI_000106"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8441192.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46897)
                     /locus_tag="ACHARI_000107"
     CDS             complement(45014..46897)
                     /locus_tag="ACHARI_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8441193.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(47060..49138)
                     /locus_tag="ACHARI_000108"
     CDS             complement(47060..49138)
                     /locus_tag="ACHARI_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8441194.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="ACHARI_000109"
     CDS             complement(49151..49426)
                     /locus_tag="ACHARI_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441195.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50242)
                     /locus_tag="ACHARI_000110"
                     /pseudo
     CDS             complement(49788..50242)
                     /locus_tag="ACHARI_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(50396..51604)
                     /locus_tag="ACHARI_000111"
     CDS             complement(50396..51604)
                     /locus_tag="ACHARI_000111"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8441196.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51716..52048)
                     /locus_tag="ACHARI_000112"
     CDS             complement(51716..52048)
                     /locus_tag="ACHARI_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8441197.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52196..53071
                     /locus_tag="ACHARI_000113"
     CDS             52196..53071
                     /locus_tag="ACHARI_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8441198.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53153..53734)
                     /locus_tag="ACHARI_000114"
     CDS             complement(53153..53734)
                     /locus_tag="ACHARI_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8441199.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53740..54999)
                     /locus_tag="ACHARI_000115"
     CDS             complement(53740..54999)
                     /locus_tag="ACHARI_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8441200.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55001..56047)
                     /locus_tag="ACHARI_000116"
     CDS             complement(55001..56047)
                     /locus_tag="ACHARI_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441201.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56282..56557)
                     /locus_tag="ACHARI_000117"
     CDS             complement(56282..56557)
                     /locus_tag="ACHARI_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8441202.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56800..58110)
                     /gene="der"
                     /locus_tag="ACHARI_000118"
     CDS             complement(56800..58110)
                     /gene="der"
                     /locus_tag="ACHARI_000118"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8441203.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58292..59521)
                     /gene="rpsA"
                     /locus_tag="ACHARI_000119"
     CDS             complement(58292..59521)
                     /gene="rpsA"
                     /locus_tag="ACHARI_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8441204.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59656..60336)
                     /gene="cmk"
                     /locus_tag="ACHARI_000120"
     CDS             complement(59656..60336)
                     /gene="cmk"
                     /locus_tag="ACHARI_000120"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8441205.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60385..61020)
                     /locus_tag="ACHARI_000121"
     CDS             complement(60385..61020)
                     /locus_tag="ACHARI_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8441206.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61086..62489)
                     /locus_tag="ACHARI_000122"
     CDS             complement(61086..62489)
                     /locus_tag="ACHARI_000122"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8441207.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(62476..63507)
                     /locus_tag="ACHARI_000123"
     CDS             complement(62476..63507)
                     /locus_tag="ACHARI_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441208.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63538..63759
                     /locus_tag="ACHARI_000124"
     CDS             63538..63759
                     /locus_tag="ACHARI_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8441209.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63924..64661)
                     /locus_tag="ACHARI_000125"
     CDS             complement(63924..64661)
                     /locus_tag="ACHARI_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8441210.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64814..66154)
                     /locus_tag="ACHARI_000126"
     CDS             complement(64814..66154)
                     /locus_tag="ACHARI_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8441211.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66391..67914)
                     /locus_tag="ACHARI_000127"
     CDS             complement(66391..67914)
                     /locus_tag="ACHARI_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8441212.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT        18818 a        14345 c        11613 g        23355 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 tttccctgat ttttgttgat tgatattgat tgtctaatcc ttctgttcct ttatctagat
    49921 atgggaagta cacaagatca ggtgtgccat ttcggtaaag gatttccatt cgctcgatat
    49981 cttcgtattt caatgcgcga ttaaacatgc ccatttccag tccgcctagg ttgatatcat
    50041 gtgtcgcaac tgcgtcccct tcatggaaga tttcgatttt aaatccagca cacggatgga
    50101 tttcaacaaa ttcactgcca ttgattcgtc caaaacttgt tgtgatttgt ttgatccaaa
    50161 gatcgccgat atgacggcgg ttgatcgtcc aggtttctcc atttctaaaa taaaatttta
    50221 gcgctgtcat ttctttactc attaaaaaga ctcctttatg atgtaattta ttcttgtact
    50281 acctcagttt agcacaaatt tatgtaagtg gtcacatctt atgttttttc ttctaatgac
    50341 gtatctgccc actttaagta gccaacaaag ctgagcagat acagtctagt gttttttaaa
    50401 tcaatttttc tttcgataaa aaatccaaaa cctgtttttt atcatttttt attctttgtt
    50461 ctacgacggc tttttcagct aattgcaaag cttcactaac ccacttacca ggtttttttc
    50521 cagtttcttc tagcaagatt ttaccatcta ctgctaattc gcttaatgac ttgataggta
    50581 aacgatcata ggattctcct acggcatcca tctgaggtgt ttcgtcaaaa tagatcatca
    50641 attcttctac ttgcagtgca tgttttttgc ctaagaagta caattcgaat ggttccaaag
    50701 ctccagtttc tcgttgtcgt agtccttgga caagtgcttg aacgtcttta atcatttggt
    50761 tagaacactt ccacgatttc aaaaaggaac gaatctcatt ttcttttaga tccagttggc
    50821 tgatcaataa tgcccatgct tggctctctt ctttcaatgg gacggatggt aattcagcaa
    50881 aacgtaaaag tgcttcgcct tcttctttca aaccgggaca gcaaatgtag cactctgttt
    50941 caataaatgc atggattccc gcggcccgtt catttcccaa gagcatcttg atgaattcca
    51001 catttatacg ttctacagat attttaccta atagctcgtg gttttctttg atggcgtaga
    51061 aggtttcttc ctctaatttg aaccctagtt gactgacaaa acgcaacccg cgcatcattc
    51121 gtaaggcatc ttcatggaac cgctcatgag gattgcctac agctcgcaag atctgttctt
    51181 ctagatcttg cagaccatcg aacaaatcga tgatttctcc atcttctttc aaagcaaacg
    51241 cattgatcgt aaaatctctt cttttcaaat cttctgccaa cgaacgaacg aattcgacat
    51301 gatctggtct acggtagtcc tgataagttg attccgtccg aaatgtggtg atctcatatt
    51361 gttcatcatt ttctaatacc aacacagttc catgttcgat cccaatatct atcgtttttg
    51421 gaaaaagctg tttgatttct tctggaaatg cgcttgttgc gatatccaca tcatggattg
    51481 gctggttcag caagatatca cgaacacttc cacctacaaa ataagcttca tatcctgcat
    51541 tctctaattt tttcagaact ggaattgcct tttggtattc cagaggtaat tgttttaatt
    51601 tcatttttcc aacctttcct gcgtgtttct tgtcctgaga taaagactca gttgcatggt
    51661 gactgtttga caagtccgtt tcagagtctt caaggccagg accacttcat cttgtttact
    51721 cgtccttacg ctcaaagcga ttatggtctc tttgattgaa tttttccatg tttttttcaa
    51781 aaacttcagt taagtcgata ttcaatgaat tagccatgat catcgtaacg aataaaacat
    51841 caccaagttc ttctgctacc gagttaactg gttcagaagc ctttttgctt ttttctccat
    51901 aagtgtggtt gacttctctt gccaattcac ctacttcttc agttaatcga gccatctgag
    51961 ctaatggaga gaaatacccc accttgaatt gctggatata ttcatccact tcctgctgca
    52021 tactgtataa agaacgcttt ttatccatct ttttccctcc aaaactatct taccaaaact
    52081 ttaacgcaaa tcctagataa gagcaattga gaaaatcgaa tttccatgct acatttagga
    52141 aggaatttct aaatggaaat tctgtgaaaa tacataattg aaagctggaa ttcatatggt
    52201 caaacgaaaa acagtaaaag aagttctttt tatcattata ggaacaagta tttatgcatt
    52261 tggtcttgtt tacttgaaca tcgctaatca tttagcagaa gggggagtct cagggatcac
    52321 cttgatctta agagctttat ttggtattga tccagcttat acgactttat taatcaacat
    52381 cccattgatt ttactaggag gaaagatcct cggtaaacgt tccttggcct atactgttct
    52441 aggtacactt tctttgtcat tctttttatg gatctggcaa agggttccac tagaaatcaa
    52501 tttacagcat gatctactga tcgtttctct acttgcgggt ttagttgcag gtgtaggaag
    52561 cgggatcgtc taccgcatgg gtggaacaac aggcggcagt gatattatcg ctcggatttt
    52621 agaacaaaat tacggcatca ctatggggcg ttccctattg gcatttgata tcatcgtctt
    52681 acttgcttca ttaacctata tcgacttgaa acgtatgatg tatacgttga ttgtttcgta
    52741 tgttttcagt cgtgtgattg attcgatctt agatggcgga tattctgcaa aaggtctact
    52801 tgtcgtctcc aacaaaagcg aagagattgc accactactg atgaccggtc tgcaaagagg
    52861 tgtgactttc ctttctggag aaggcgcatt ctctggtaat tcaaaaaaaa tcatctatat
    52921 ggtcgttagc tctagtgaat taactgaagc taagaggatc gtccacgaga ttgacgaaaa
    52981 agctttcctt tcaatcatca atgtgcatga agtggaaggg caaggcttta cttatttgaa
    53041 gccacagaca agattactga aaaaactata agaaaaaaga caaagatagg ctatgaggaa
    53101 agattccaca tccagctgac ctttgtcttt tttagtacta tactattttt aactaattgt
    53161 tatgttcact tacccggtta cttttctttt gtgtctgcaa aaggatcttt cggttaactt
    53221 catcagataa tttcaaaaaa gcatccctat ctccatcttc tagtgcttga tcgatttgag
    53281 cataaagcat ttttagtttt gcctcttctt ctttttcttg aaaatatcgt tcaattaatt
    53341 cagtatcttc ttcactgact gaagaatccc aagaagaaaa tggattgtct tctaagatag
    53401 atagatattc tcttgtctgc caagcatttt cgaatataca ttccacatat aaaggctttt
    53461 tccaattcaa acggatttca tgaaaaatct ggtctgtgtc cgtaaattct tttccctgca
    53521 aaaacaattt catgggttcc ccttcaacag atagatcact gatctgcagt cctcgatccg
    53581 caaaattcgc cccttcaaca aagtggacat tgcttaaaat cgcttcatga ttggcaagat
    53641 aattcaagat ccaaaaaact tctcttcggc taaaagatac atgattgacc atccacgtta
    53701 aaaaattctt cttttcccgt acatcaatta gcatttttct cacctctctg ttaggtcatc
    53761 taataatgac agtgcctcca tatctcctgg ttcaagtgtt aaataataag tcaataattc
    53821 ttgcgctctt ttcagctgcc cttcttctct taagaaaaag gcatactctt tcaagaaatc
    53881 tggttcatgt gatagttcat gataagcctg ttcatagtgt acagctgcaa cggtaaagtc
    53941 ttctaattca ttataagcat gggctaggtt ccattctgca taaggattag cagtttcttc
    54001 catctgattg atcacttgta tcacatcttc atatctttca tcatccaagt aaagattact
    54061 taaagtaagt aatgtttcat cttgtttgtc tcctacttct aaagctttca gtaaaagttc
    54121 ttctgctcgc tgtctatcat gcagacggaa agcattttcc gaggccaggt gataaagttc
    54181 gacttggtaa ggattttctt tgattccttc ttctaacact gtttgtgctt cttcaatcat
    54241 ttcttcttct tgaagagctt gtcctagata tagatataac gattgataat gcggattaac
    54301 ttctcgaagt tcttccagat agttgattgc ctgctgattt tctttcaatt gaagattcgt
    54361 aaaagctagc tggaacagtc gatcatctgt cctttcttct ttcagtgctt cttctaaagg
    54421 tacgatagct gcttcgaatt ctccttgcat acttaaggat tgtcctaaac gttcctgcat
    54481 cgagattcct gaaatttcat ttatcccgct tgccaacaat gtttcataaa ccactgctgc
    54541 ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc gcaaactgga tcaatggttc
    54601 atcaggaagc aacccagctg cttctttcaa ttttgcttca ctcacttcag ggattccgat
    54661 aacttggtac aaatcagcta tcgccaataa gctttgcgga tagtaatcac tttgcttatc
    54721 cactttttcc aggtattcaa aagctaagtc gatctcatta tcttcgattg caatttctgc
    54781 taacgggatg ttcagtccgt cattttctgg gtattggttg atcagttgtt caaaaatctg
    54841 ttttgcttcg atcaaaaagc caatagccag caattcttcg cctagttcag ctaatatatc
    54901 tgctgggtct tcttttaatg ctttttctaa aaagatttgt gcttgtgaca agtcttcatc
    54961 tgataaagct tgcaacattt tttcgctatt tgtattcatt tcaattccct cttatctcac
    55021 tgacgatttg ttgaaatacc gtcattgttc tcaataattc tttttcgttt ggatgttggt
    55081 atccgtctat tatacctatt ttttctaaaa caagtatttt tttgcttttt tctgccaaaa
    55141 aaagaacttt ttccaaatac tccatctttg aaatctgttc cagccagtgg attggatagc
    55201 caagcaagtc cagccacaca aaaaagtttt ttagttggaa atcaaaagga tatgcttgga
    55261 gattccacac gaaatgaaac atcattccta aaaatttttt catactagca gaaagtagat
    55321 ggccattttc ggtcagataa aaagcttgtt caaacagttt accataattt gacaggtttt
    55381 ctgcctctaa ttgataaaaa tagatcattt cttcgatcat cccggcaaac ggccgagtcg
    55441 ttaattgctt tttatttgga tagtttttat acagattttg caaaaaacga tgatcacaaa
    55501 tcattccgca acagataaaa gtcaaaaagt caatctgctt tccgtccacc tgccgctcgc
    55561 tgattgtttg atcataaacg atgcgttctg gcagattgat ggtttgcagc actacacgat
    55621 ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg gcttagagaa cggatcgata
    55681 cagggatcat ccatagtgat gaatcaaggg ttgtatgttt ttgaaaaaaa cctgccagat
    55741 ccatgatccc ttcatttcca aatccgatga taaacatgga ttgattttcg ggatagcgtt
    55801 tgacgaaatc aagcaggttc tttaattctg ttaaatgatt gcattgagtg ttggcacaaa
    55861 tataccagtc attgtttgct tgatttgcaa ataacgggtt gatcttatct gcaaacaaat
    55921 cataatagcg ctgattcgtt aaaaataaga ttggctgatc aatggaaagt tctcttagct
    55981 gactgactag cgtttctccg taaaacactt tcgtcaacgc atcttctact tgataatcaa
    56041 gttccatacg catccctcct cttcttattt ttattttacc attagtaagc aaattctaga
    56101 aaagataaga gcaaagctca cctcataact tgcaaataaa taaaaattaa ataaaataca
    56161 tgtgttttct atgtatgaca tacggattcg gaatctctgt ctcttattta ttccttttct
    56221 gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc acaagcgctt tttcaactgt
    56281 cttatttaac agcgtctttt aatgctttac ctggtttgaa tgcaggtact ttgctagctg
    56341 gaatttcgat ttcttcacca gtttgtgggt tacgaccttt gcgagctgcg cgttcgcgaa
    56401 cttcaaagtt accaaaaccg attaattgaa ctttttcgcc gttagctaaa gcatcttgaa
    56461 tagttgaaaa tacagcatct actgctgcag ttgcatcttt tttagttaaa tcagtagctg
    56521 aagcaacttt ttcgattaat tctgctttgt ttgccatgga tagattcacc tcctccacaa
    56581 gagatatgat agaaatatca tttagttaaa actttctgag tggtccagaa gtttaaaaag
    56641 aaatattgat taacttcaat attctgttat cacgatatca tatgaaccgc ttaatagcaa
    56701 ggtttttacc aattttcaca tgcttttttt agtcatagaa acgtaacaaa cgaaactgtc
    56761 tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc tattttctcc tacgagggat
    56821 aattcggatg gacgtccctt caaaagtaaa tgcttttctg atttgatttt ctaagaaacg
    56881 ttcataagaa aaatgcatca attcctcttc attgacaaaa acgacaaatg taggtggttt
    56941 gacagcgact tgtgtagcat aaaaaatctt caggcgtttt cctttatctg ttggtgttgg
    57001 attgattgct acagcatcca taatgatatc attcaacaca gccgaagaga tacgaagatt
    57061 ctggttcata cttacttgtt cgatcatttc aggcagacgt tccaaccgtt gtttagtcac
    57121 tgcagaaaca aaaacgattg gagcgtaatc aagataccgg aattcatcac gaatctcttg
    57181 ttcgaaatca cgcattgtat tggtttcttt ctttacaaga tcccatttat tcacgacgat
    57241 aataattcca cgaccagctt catgggcgta acccgctact cgcttgtctt gttcccgaat
    57301 cccttcttca gcattcagta ccatcaaaac gatatcagaa cggtcaatgg cacgcattgc
    57361 acgcatcacg ctgtattttt cagtattttc gtatactttt ccgcgtttac gcataccagc
    57421 tgtatctatc attaagaatt tctgaccact ttcagaaacg aagtgggtgt cgattgcgtc
    57481 tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct tctcctaaaa tcgcattgat
    57541 caaagaggat ttaccaacat ttgggcgacc aatcaaacta aacttaatcg tgtcgtcgtc
    57601 ctcttcttca gtctcttcag agaaatactt cactgcttca tctaaaatat caccaatccc
    57661 tagtccatgg cttcctgaaa caggataagg atcgcctagc cctaaggaat aaaattcata
    57721 gatatcgttt ctcatttctg gattatctac tttattgact gctaaaatca ctggtttatt
    57781 acttctgtat aaaattttcg caaccatctc atctgcgtct gtcacgcctt cgcgtccgct
    57841 gactacacaa atgattacat ctgcttcttc aatcgcgatt tctgcttgat gcttgatctg
    57901 atccataaaa ggttcatcgc caagatcaat tccgcctgtg tcgatcacgc tgaattctcg
    57961 acctaaccat tcaccttttg cataaatacg atcacgagtt actccaggag tatcttcgac
    58021 aatcgagata cgctcaccag cgatgcggtt aaaaatcgta gacttaccga cgttcgggcg
    58081 gccaacgatt gcaattgttg gatttgccat attatttcct cctcacaagt ttccaatctc
    58141 acttagttta acgaatgcat tgccaacttt caagcataga ttgtcaatta aacgaagtat
    58201 ttttattcct tattgaaata ctaatcgctt ttcaaaagaa aaactgagac aaaagcagct
    58261 gctttcatct cagtcacaga tacaaaatca attatttttc agaatcatcg gttgcttttt
    58321 cttctacttc ttctttcaac gcatcaccta aaatgtctcc catagtaaat ccagtatttt
    58381 cttctggtaa ttcataagac tcaacatttt ttggttcttc ttctgaagct ggtttttctt
    58441 ctaaagcttt gatactcaat gcaatacgat gttcttctgg atggatctct aatactttca
    58501 ctttcacttc gtcaccttca tgaagcactt catgtggtgt cgcaatatgt ttatgtgaaa
    58561 tttgagagat atggactagc ccttctactc cagggaacac ttcaacaaag gcaccgaagc
    58621 ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac tggagctttt gtatcgattt
    58681 ctgaccaagg tccaggctgt gtgtctttga ttgacaagga aacacgttct tgttcaggat
    58741 tgatagacaa cactttgaca tttacatcat cattgacagc caatacatca cttggttttg
    58801 ctacatgact atgtgaaatc tctgagacat ggactagacc gtcaattccg cctaggtcga
    58861 caaatgcgcc aaaatcagta agacgtgcaa cacgtccact gagaacatct ccctcatgga
    58921 tagttgaaag aatttctttt ttcttttctt ctctttcgtt ttccactacc gctttatgcg
    58981 ataagatcaa acgattttct gatggttcga tttcgatgat tttaaagctc aatgttttgc
    59041 ctttatagtc tgaaaagtct gcaacaaaat gatcttccac cattgatgca gggacaaaac
    59101 cacgtacacc gacgtcaacg actaaaccgc ctttgacaac atttgtaaca ggtgcttcga
    59161 tgaccttgcc ctcttggaaa tctctttcga tatcttccca aactttttta gcatctaaac
    59221 ggcgttttga aagtagatag ctgccgtttt ctttatcttt gccgatcgta ctgataacga
    59281 ctaaatctaa tacatcaccg acttttacta cttcgctgat atcttctacc ggcaaagttg
    59341 ataattcttt tgccggaacg actccttcaa ctccagcccc ttcgattccg acaatcactt
    59401 gtttgtcttc aatgactaaa acttcgccct taacaacatc gccaactttt acttcttgta
    59461 cgcttttcat tgcgtcttcc attgactggt tgttctcttg attttgatca aattctgtca
    59521 taacacttgt cctcctaacc aacattctgc cgtaagtacg gtactgtttt tagtttaatt
    59581 gattattcaa aaaaaataaa gcgatttcgt tcgttttcct tgaaaaaaca attgtttttc
    59641 attttttttc ttaatttaaa aaagaagata accttttttc aaaacaacag cttttattgc
    59701 tgcaacgact tctggaatcg atttgccagt cgtatcgata cgaacagcat cctctgcttg
    59761 cttcagcgga gagacgtccc ttgttgaatc ataatggtct ctttcctgta tctctttcgt
    59821 cagtgtttca agatcagtcg gaattccttt ttcttggttc tctttgaaac gtctttctgc
    59881 acgctcactt acgcttgcta ctaaaaagat tttcacttct gcattcggta atacagctgt
    59941 cccgatgtct cttccgtcca tgacaacgcc accgcgttcc cctatttttc gttggacagc
    60001 aactaattct tctctgactt ttccatgtgc agagacttca gaaacagctt ttgtgacatc
    60061 tggttgacgg atttctaaag tcacatcttt tgatgctatg aatacatgct gtccatcttc
    60121 ttgttgcgaa aaagagatag gatacttttt gatcagcgaa actagccctt cttcatctga
    60181 aaaagataca ttgttcgtaa tagctaaata agttactgcg cgatacatgg caccggtatc
    60241 tgtataaata taattcaatt cttttgctaa tatcttggca acggtacttt tcccagatga
    60301 agcaggcccg tcgattgcaa tactgatttg tgtcatgata taactccttt ttctcagaaa
    60361 aaaagccgca aagcgcggct attattattt aattcttaat tcttgaccag ggtaaagcat
    60421 aaagttgttt ggatctaagc cattcaattc aaacaattga tcagtggtga tccctgcacg
    60481 ctcagccact tgttttggac cttcgcctgc ttgaacagtc gttgttgaac cggctgcagc
    60541 tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa tcccctgctt gataagttgc
    60601 ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg ctttctgacg ttacacctgt
    60661 atcttgagac acagtagatt cttccgcgat tgtactagat tctactgttg aagtctcagt
    60721 tgactcctct tttgttgtac ttgtagaact ttctactact gatgtcgaag tttcagaaga
    60781 agttgctgct gttgcattgt tgcttgatcc atttctgatc cagaaataag cacctgctgg
    60841 aatagcgata cataagacca gtaaaatcaa aaatagcgaa agaaataatg tatttccttt
    60901 cttttgttgt ctctgagaac ttcttgatga aacttcatca tctgtatcgt agattggttg
    60961 ttcccatggt tcttgtgttt cgttattttc tgtagattga tgtctatctt ttctgctcac
    61021 tgttttacct cctaaactct atcgtgtgta ttataccata ggaaaaagtg tcttgtccta
    61081 aaaaatcaat gttccatgtt ttttttaaat aaatttagta aaatcgttcc ccaatgaagt
    61141 tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc tccatcaata
    61201 tcacaacaat tctgcggctt ttcggagagt ttttcgccaa aataagctaa gatgaatttt
    61261 ctgcggcagt tttcttcatt aatatagcga agcatctgct gcaggcggaa ttctttttct
    61321 ttttcctgac gtttgagccg ttccacccaa ttttctggtt tttcagactg ctggatcagc
    61381 tccatccatt ttttttgcag ctcatcaaag ggagcttgct ctgtggcata ttccaaataa
    61441 agctcaaaac tttgtcgctg ttctctagaa agttgattaa agaaataatg gatacgttca
    61501 tccccacttt gataaaaaag aattgctgca ctttcttcct gatctctgcc tgctcgaccg
    61561 atttcttgta catagttttc tagactgtct ggcaaatcat aatggaccac ataacggata
    61621 tcagatttat cgatccccat accaaatgca ttcgtagcaa tgagaaactg cagctgattt
    61681 tttacaaact gctgttgaag ttgtcttctt tgagctgcat caagaccacc gtgatagtat
    61741 ccaactgtaa aacgttccct gaataaatga tacaatcgtt cgacttcttt ttttgttgca
    61801 caataaatga ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc ttgttctttt
    61861 tgctgcgttt tccgaacaaa aagcgagata ttttgccgat tgactgattg tctgatctct
    61921 tttggtggtc gatcaaatag tacatgacgt atatctcttg ctactaaatc tgttgctgtc
    61981 gccgttagtg ccagtgtgac cgggtttcct aaaagctttc ggatttttcc taactgttga
    62041 tattctggac ggaaatcaac tccccattga gacacgcaat gagcctcatc tactacatat
    62101 aacgcaatat tttgtttttt cagatgctct aaaagagaag gattagtcaa catttcggga
    62161 ctcaaaaaca aaaatttata ttgcgataga tgacggagga cgtatcttct ttcagattgc
    62221 gaaagtaaac tattgaaagc agctactcgt ttctcccctc gtttttgtaa ttgcgtcact
    62281 tgatcttcca ttagagaaag cagcggtgac acgatgatga ccagaccttc cattaaatac
    62341 cccgtcaatt gatagcaaag gctttttccc gttcctgtag gcaaaatagc gagagtatct
    62401 tctccatcaa gcaacgcctg aataatttct tcttggccgg gacgaaatga atcaaatcca
    62461 aaatattttt ttaattcatt tattaacatt ctgtatcgct tcctttctca atagatagat
    62521 ttgatacaaa cgaaattctc cataatctaa ttgtccatct acattccatt cgctaaagcg
    62581 ccactctcgc acggaatcct tatttggttc caaacgttcg attgtttctg cagagagaat
    62641 ccactcgtat ggaaaatcat caaaaaacaa ggcccactca ataagatgat ctgttactgt
    62701 tcctcttttt aaatgacgga ttgccatgat ctggtcaatt gtctgaccat tcataaacat
    62761 ctgcttggtg atcatcatgc tttgattgaa attttgctgt aataatgaat cgatcaaagc
    62821 gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac aaaaggtgga tacatctgct
    62881 ttgttgcaga taaaccgctg actcatcatt gttttttgcc agctgataag gaagcagccc
    62941 tgttcgctca tttccagata attggttagc taaaaaatct gctgcttctt ctggtatctc
    63001 cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc aaaatttcac gtggcaattg
    63061 gctttcacta agccattttt ttagttggaa agtataaaaa ggacttgtct ctgctggaag
    63121 atagtcttga atacccgttg ccagattaga aattacttga acagcaaatt taatgagccg
    63181 ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc aaaccagtat gttctatttg
    63241 atgttcggac aacatatcag cccctgcagg cgttagcttt gcttcatttt catttatttc
    63301 gatccaacct tgctgcgcaa ttttttgcaa aatttgataa aacttatcct gcttcaattc
    63361 tggaaaactg ttatggataa acaaaagttc gtgggtaaat ccataaataa gtactgaagt
    63421 cgttcgtttt cccacaagga gttggtaaag agaactaatc cgcagcttgt gtcctgatga
    63481 aaatagcgat aaaataaaaa caatcatatt attctcctta agttaggatg gttagaaatg
    63541 tcactactat gtaaacttgt acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa
    63601 gcgccagata ttttcgatta tgatgaagaa ggaatcgttt tatttgccca agaaccagag
    63661 gctcatcaac agttcgtgat gccggaagaa gaaacatatg taaaaaaagc atatcaaaaa
    63721 tgtccagttc gagcgatttt attagaaaat aaaatataaa tataaacgaa actttaaaaa
    63781 cgtacactaa gttctctaaa cttgaaaata agccgggatc tgtttctctt gttccgtgaa
    63841 atcccgactt attttgaaag attcatttgc tatctatagt taattgacta gttttttcac
    63901 ggaccctata ggacccattt atattatttc aactttttca gccagttttc caatgtattg
    63961 acgatcacag tttgctggtc tgcgttagaa atttctgcct ctccatctcc tttttgctca
    64021 ccatagctgc caaaacctcc atgatttccg ccattgattg atacatactc tgttctttcc
    64081 ggtaaatact gcttagcttc ttgataagct tcctgattca atactttatc ttttgtcgcc
    64141 gtgatcgata aaacaggaac agctgattct gtcaatgttc cttttttgtc gggataactt
    64201 gccatgaaga ataccccttt taaatgtttg tctgaatgat tcttattgac ataacgactt
    64261 gccattacgc cacctaatga atggccaccg atcacataat cggaatttgg aaaatcttct
    64321 ttgaccttat ccgctttgtt tccgcccatc actgctagat caagtggcat tttgacaata
    64381 gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa tactataact ttctggctct
    64441 acgagagcac caggataaaa aataagtaat ggagactttc catgattctt tttctctcct
    64501 tcaaaaaata agtaatcttc tttgtttttt ccttctttta ccaacacttg cgcttctgac
    64561 gatggttcgt acgtattttg ctgtatatag taataaccgg ctccaccgag taatcctaaa
    64621 atgatgacta agccaattag tattcgtcta aatattttca ctttctccca ccttttctat
    64681 ctgagtgacc cctttttctt aatcaagtat gattatacct taaaaaaata agagagaaat
    64741 gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc atttgaatat ccgccctttt
    64801 tgtatgttta tttttattta gcacataaac gatacacagc ttctgcataa atttccattg
    64861 ctttatacat actacttaat tcccatcgtt cattcgcctg gtgcataaag tcaggagtat
    64921 cagggaacat cgcaccgaat gccacacact ggttcatcgt acgagcaaaa gtagcgccgc
    64981 ctgatacaaa cggttcagtc atgtctccag taatatcgcg atatgtcccc aatagatttt
    65041 tcactaactc actgtctagt ggcacgtata atgaatcaag gaagtcaaat tcttcataag
    65101 tcaacccata tttttctgct gttttagtta atttagctac taaatcatct ttcttgaatg
    65161 taacagggat tctcatatct acaccgattt ttgtttgttc tggtgtgatt tccaaacttg
    65221 caaagttcat cgttagctcg ccggattgtt catcttctgt tttgcccacg acagcttcac
    65281 cagttgcatt ttcttgaact aattttccta agaaattgat tgggccaaaa tcaaatactt
    65341 ctgaaagagc aattgccaga cgagaaatag cattcactcc ttgtgctgca tctttcgcgt
    65401 ggatactttt accaagaacg acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt
    65461 ttttcaatgc ttcttttact tcagaaagct tttctccaga gtaaggagct gcatcaggaa
    65521 caacattcaa cgctcctcca gcttttacag aaaactcgtt cgttcctggt cctgtcaagt
    65581 aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc
    65641 cttgcgtaat tccttcttct ttttcgttat atttttctaa acaacgccat aaattttctt
    65701 catctgtacc aaagataaaa cgaatacgtg tcttgaattc cacgccagca tcaatcaaag
    65761 ctttgacggc atacattgcc gcaatagaag gacctttgtc atcttgggat ccgcgaccaa
    65821 ctaaccagcc gtcttttata gttggatcga atggttttgt ttcccaatta ttttcgtccc
    65881 ctgctggaac aacgtccata tgacacaaga taccgaataa ttcatctcca gagccaatct
    65941 ctgaataacc gtaatagcct tctggatctt taaatgttct aaatcctagc ttttcactga
    66001 tttccaatac tttatccaaa gcttcaatca cttttttacc gaagggatgt ccttgtcctg
    66061 aatctgcttc atctagtacg gaaggaattc ggatcaattc gctcaatgat tctaatgctt
    66121 gttcatgatg ttgttctgtc acaaattttt tcatgatatt atcctcctaa tatgttaaaa
    66181 agggtcatgt caaattcctc cgacacaacc tcttcttctc aaataatgac gttttaagcc
    66241 tgctccatca gcaagcagaa tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat
    66301 ggagctattg acttatccac gtgcttatcg atagccgttt atcggtttct tcattcttat
    66361 gatagacctt gtacctgtat ttgtctaggt ttagaacatt gctgctacac ctaggatcac
    66421 acagatggct acaaataata tcgcaattaa tttaccggta aatttccacc acactgaaac
    66481 atcgaaacga gcaattgcta cagcacccat tacgatagca gatgttggtg taattaagtt
    66541 tactaaacca gatgcagatt gataagcagt gattactaaa tccttaccga cgccggcaaa
    66601 ttcacccata ggtcccatga ttcccattgt tgcagcagct aagccggaag ttgaaggaat
    66661 caagaatgac atagggatat agaaaataaa tgtaataatg atgaatacac ttgaagataa
    66721 tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct gtgattaatc cattgttcat
    66781 aaccacttgg ataccacgag caacagctac tacgattgct acaccgatca aatcagaagc
    66841 accagctaaa aattcagaga caaattcaga ctctttcatg ccataagtga ttgctactac
    66901 gatggacatt actaagaata acatagtaat ttctgggaag taccagtcac ctaatggaag
    66961 catgctttta cctagaaccg tacctagtac cggaaggttt gttaaccagt ttgtcaatga
    67021 ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga atcaaactga tgatcatgat
    67081 accaaaagta ataaagaaca atacattaac atgtttttgt tttttcgtga ttacttcttg
    67141 acgaccgatt gcttcaatat cgaaatgctt catatcttct ttacgttggt tgtatactaa
    67201 tgattttgtt ggatcttttt cgatctttga tgcataacga tacacatata caatagaaat
    67261 agctaacaaa ataacgaaca tcaagaatcg aagaccaata ccttcaccca tcgaaatccc
    67321 aacagcttgt gaagctacgc ctgttgcaaa aggatttacc gttgaagcta gacagcctac
    67381 ttgagaccct actaatacga ttgctacagc tactactgta tcaaagccta cagccatcat
    67441 gactgggatc agtaaagcat agaatggaat cgtttcttct gccattccgt aagtagagcc
    67501 gcctaatgca aataacagca tcaatactgg aataagcatt ttttcttttc ctttgaattt
    67561 cttcacaact gaagcaatcc cggcatctag tgcccctgtt ttgttgataa tacctaaaaa
    67621 tccaccaaca accaaaatga agaaagagat tgggattgca gcaggtgttt ctttagttag
    67681 ctcccccgct ggtacaccta gcatcccatt gattggcgcc atgaaaatat cccacagccc
    67741 ttgaggattt tgttgtactc gttcatatga tccagcgacg atgttgcctg catcgtctac
    67801 actatagtgc ccagctggta taaaccaagt aagtacagcg attactgcaa taataataaa
    67861 taagaccgta tacgctgaag gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt
    67921 cttcctttct tttccgttag agacaacaat tttggctcta atgttatttt atcaaaaaga
    67981 gtatcagaac aaaaataata acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt
    68041 tccctattcc ctcctctttt ttcaggtttt taaagctttc ttctttttca ttgtagcgtt
    68101 tccttttttt tataaacaaa cattttcgtc t
//
LOCUS       DAYYAO010000003        63593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000003 DAYYAO010000000
VERSION     DAYYAO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.003"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACHARI_000128"
     CDS             complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACHARI_000128"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8441213.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(2013..2390)
                     /locus_tag="ACHARI_000129"
     CDS             complement(2013..2390)
                     /locus_tag="ACHARI_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441214.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            2646..3374
                     /gene="purC"
                     /locus_tag="ACHARI_000130"
     CDS             2646..3374
                     /gene="purC"
                     /locus_tag="ACHARI_000130"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8441215.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            3374..3628
                     /gene="purS"
                     /locus_tag="ACHARI_000131"
     CDS             3374..3628
                     /gene="purS"
                     /locus_tag="ACHARI_000131"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8441216.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            3630..4301
                     /gene="purQ"
                     /locus_tag="ACHARI_000132"
     CDS             3630..4301
                     /gene="purQ"
                     /locus_tag="ACHARI_000132"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8441217.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            4302..6524
                     /gene="purL"
                     /locus_tag="ACHARI_000133"
     CDS             4302..6524
                     /gene="purL"
                     /locus_tag="ACHARI_000133"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8441218.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            6509..7948
                     /gene="purF"
                     /locus_tag="ACHARI_000134"
     CDS             6509..7948
                     /gene="purF"
                     /locus_tag="ACHARI_000134"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8441219.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            7971..9023
                     /gene="purM"
                     /locus_tag="ACHARI_000135"
     CDS             7971..9023
                     /gene="purM"
                     /locus_tag="ACHARI_000135"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8441220.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            9020..9598
                     /gene="purN"
                     /locus_tag="ACHARI_000136"
     CDS             9020..9598
                     /gene="purN"
                     /locus_tag="ACHARI_000136"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8441221.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            9595..11130
                     /gene="purH"
                     /locus_tag="ACHARI_000137"
     CDS             9595..11130
                     /gene="purH"
                     /locus_tag="ACHARI_000137"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8441222.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            11146..12420
                     /gene="purD"
                     /locus_tag="ACHARI_000138"
     CDS             11146..12420
                     /gene="purD"
                     /locus_tag="ACHARI_000138"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8441223.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            12542..13456
                     /locus_tag="ACHARI_000139"
     CDS             12542..13456
                     /locus_tag="ACHARI_000139"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8441224.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(13589..15154)
                     /locus_tag="ACHARI_000140"
     CDS             complement(13589..15154)
                     /locus_tag="ACHARI_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441225.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(15147..16598)
                     /locus_tag="ACHARI_000141"
     CDS             complement(15147..16598)
                     /locus_tag="ACHARI_000141"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8441226.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(16612..17628)
                     /locus_tag="ACHARI_000142"
     CDS             complement(16612..17628)
                     /locus_tag="ACHARI_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8441227.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            17848..18936
                     /gene="chvE"
                     /locus_tag="ACHARI_000143"
     CDS             17848..18936
                     /gene="chvE"
                     /locus_tag="ACHARI_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8441228.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            18990..20516
                     /gene="mmsA"
                     /locus_tag="ACHARI_000144"
     CDS             18990..20516
                     /gene="mmsA"
                     /locus_tag="ACHARI_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8441229.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            20518..21723
                     /gene="mmsB"
                     /locus_tag="ACHARI_000145"
     CDS             20518..21723
                     /gene="mmsB"
                     /locus_tag="ACHARI_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8441230.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            21980..22957
                     /gene="guaC"
                     /locus_tag="ACHARI_000146"
     CDS             21980..22957
                     /gene="guaC"
                     /locus_tag="ACHARI_000146"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8441231.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            23221..24645
                     /locus_tag="ACHARI_000147"
     CDS             23221..24645
                     /locus_tag="ACHARI_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8441232.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(24687..24944)
                     /locus_tag="ACHARI_000148"
     CDS             complement(24687..24944)
                     /locus_tag="ACHARI_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441233.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            25106..26215
                     /gene="pdhA"
                     /locus_tag="ACHARI_000149"
     CDS             25106..26215
                     /gene="pdhA"
                     /locus_tag="ACHARI_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8441234.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            26218..27195
                     /locus_tag="ACHARI_000150"
     CDS             26218..27195
                     /locus_tag="ACHARI_000150"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8441235.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            27220..28863
                     /locus_tag="ACHARI_000151"
     CDS             27220..28863
                     /locus_tag="ACHARI_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8441236.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            28871..30277
                     /gene="lpdA"
                     /locus_tag="ACHARI_000152"
     CDS             28871..30277
                     /gene="lpdA"
                     /locus_tag="ACHARI_000152"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8441237.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            30439..30714
                     /locus_tag="ACHARI_000153"
     CDS             30439..30714
                     /locus_tag="ACHARI_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8441238.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            30720..31487
                     /locus_tag="ACHARI_000154"
     CDS             30720..31487
                     /locus_tag="ACHARI_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8441239.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            31655..33484
                     /gene="typA"
                     /locus_tag="ACHARI_000155"
     CDS             31655..33484
                     /gene="typA"
                     /locus_tag="ACHARI_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8441240.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            33745..35517
                     /gene="recQ"
                     /locus_tag="ACHARI_000156"
     CDS             33745..35517
                     /gene="recQ"
                     /locus_tag="ACHARI_000156"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8441241.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            35631..35933
                     /locus_tag="ACHARI_000157"
     CDS             35631..35933
                     /locus_tag="ACHARI_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8441242.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            35936..37099
                     /locus_tag="ACHARI_000158"
     CDS             35936..37099
                     /locus_tag="ACHARI_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8441243.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            37125..40553
                     /locus_tag="ACHARI_000159"
     CDS             37125..40553
                     /locus_tag="ACHARI_000159"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8441244.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            40574..41698
                     /locus_tag="ACHARI_000160"
     CDS             40574..41698
                     /locus_tag="ACHARI_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8441245.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            41679..42125
                     /locus_tag="ACHARI_000161"
     CDS             41679..42125
                     /locus_tag="ACHARI_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8441246.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            42109..42447
                     /locus_tag="ACHARI_000162"
     CDS             42109..42447
                     /locus_tag="ACHARI_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8441247.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            42566..43126
                     /gene="rsmD"
                     /locus_tag="ACHARI_000163"
     CDS             42566..43126
                     /gene="rsmD"
                     /locus_tag="ACHARI_000163"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8441248.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            43123..43614
                     /gene="coaD"
                     /locus_tag="ACHARI_000164"
     CDS             43123..43614
                     /gene="coaD"
                     /locus_tag="ACHARI_000164"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8441249.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            43604..44647
                     /locus_tag="ACHARI_000165"
     CDS             43604..44647
                     /locus_tag="ACHARI_000165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8441250.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            44806..45534
                     /locus_tag="ACHARI_000166"
     CDS             44806..45534
                     /locus_tag="ACHARI_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8441251.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            45650..45895
                     /locus_tag="ACHARI_000167"
     CDS             45650..45895
                     /locus_tag="ACHARI_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8441252.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            45892..46110
                     /locus_tag="ACHARI_000168"
     CDS             45892..46110
                     /locus_tag="ACHARI_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8441253.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            46246..46785
                     /locus_tag="ACHARI_000169"
     CDS             46246..46785
                     /locus_tag="ACHARI_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8441254.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            46778..49051
                     /locus_tag="ACHARI_000170"
     CDS             46778..49051
                     /locus_tag="ACHARI_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8441255.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            49089..50135
                     /gene="holA"
                     /locus_tag="ACHARI_000171"
     CDS             49089..50135
                     /gene="holA"
                     /locus_tag="ACHARI_000171"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8441256.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(50224..50478)
                     /gene="rpsT"
                     /locus_tag="ACHARI_000172"
     CDS             complement(50224..50478)
                     /gene="rpsT"
                     /locus_tag="ACHARI_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8441257.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(50878..51147)
                     /locus_tag="ACHARI_000173"
     CDS             complement(50878..51147)
                     /locus_tag="ACHARI_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441258.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(51409..51537)
                     /locus_tag="ACHARI_000174"
     CDS             complement(51409..51537)
                     /locus_tag="ACHARI_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441259.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(51617..52072)
                     /locus_tag="ACHARI_000175"
     CDS             complement(51617..52072)
                     /locus_tag="ACHARI_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8441260.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(52210..54138)
                     /locus_tag="ACHARI_000176"
     CDS             complement(52210..54138)
                     /locus_tag="ACHARI_000176"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8441261.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(54335..55168)
                     /locus_tag="ACHARI_000177"
     CDS             complement(54335..55168)
                     /locus_tag="ACHARI_000177"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8441262.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(55360..56064)
                     /locus_tag="ACHARI_000178"
     CDS             complement(55360..56064)
                     /locus_tag="ACHARI_000178"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8441263.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(56087..56713)
                     /locus_tag="ACHARI_000179"
     CDS             complement(56087..56713)
                     /locus_tag="ACHARI_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8441264.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            57039..59414
                     /locus_tag="ACHARI_000180"
     CDS             57039..59414
                     /locus_tag="ACHARI_000180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8441265.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(59438..59929)
                     /locus_tag="ACHARI_000181"
     CDS             complement(59438..59929)
                     /locus_tag="ACHARI_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8441266.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            60116..61036
                     /locus_tag="ACHARI_000182"
     CDS             60116..61036
                     /locus_tag="ACHARI_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8441267.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            61190..61534
                     /locus_tag="ACHARI_000183"
     CDS             61190..61534
                     /locus_tag="ACHARI_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8441268.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(61724..62224)
                     /locus_tag="ACHARI_000184"
     CDS             complement(61724..62224)
                     /locus_tag="ACHARI_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8441269.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            62752..62835
                     /locus_tag="ACHARI_000185"
     tRNA            62752..62835
                     /locus_tag="ACHARI_000185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62786..62788,aa:Leu,seq:cag)
     gene            complement(63159..>63593)
                     /locus_tag="ACHARI_000186"
     CDS             complement(63159..>63593)
                     /locus_tag="ACHARI_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8441270.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20664 a        11116 c        13565 g        18248 t
ORIGIN      
        1 aactgacctt ccgcccctct cacaccaccg tacgtacgga cccgtatacg gcggttcaat
       61 aagttgagtg tacgaactgg tacttcaagg agatgtcctc atatccttgg cgggccagtt
      121 ctttgtctgt taatgatcga tgaagaatat gacttttgga gactgaccaa ataccttttc
      181 ttgtatttga ccattcatac gctttcgctt tatcgatccc taaacgaatc agattttttc
      241 ttttggttct tggattcttc cattgtttcc aaatatattg tctgattctt cgcttcaacc
      301 aaccgtttaa ctccctcatg aattctttca tctcaccaat tccatagtag ttgatccaac
      361 cagtcattag ttgcttgatt tcttttaaaa taagttggat acttcttcca cggtttcttt
      421 tagtgagttg tttgagcttg cttttcacac gctgttttgc ttttgcgtga gggcgaattt
      481 taataccttt ctttgttggc agaatgcaga atccaagaaa ctttcgtttc aatggacttc
      541 ctaccgcact cttttctcga tttaccgtca actttagttg ttcttcgaga aattgaatgg
      601 atttcttcat cacacgctgt cctgctcgtt tgcttctaac atagatattg cagtcatcgg
      661 cgtatcgaac aaatttatgt cctagatttt ctagcacttg atcaaattcg ttcaggtaga
      721 tgttacttaa caggggagag agattgcctc cttggggcgt tccttcttca gttgttgtga
      781 tgagtccatt ttccattaca ccactcatta agtattgacg gatcagtttt aggacttgtt
      841 tgtcctttac tttccgctca atgaaataca tcagcttatc gtgatttacg gtgtcaaagt
      901 aggctttcat atcaatgtcc accacatatc ggtaaccttg ttcataataa gattttgctt
      961 tcttcactgc gtcatgtgcg ctgcgatttg ggcgaaaccc ataactatta tctgagaatt
     1021 ctggttcaaa tagcggttgt aacacttgat tgattgcctg ttgaagcata cgatcaataa
     1081 ccgtgggaat tcctagtttt cttacaccgc cgtttggttt cggtatttct actcgcaaaa
     1141 caggctgagg tttatagttc ccttggcgta aattggatag taattcctcg cggtgctctt
     1201 tcaagtaagg aagcagttga tcgacggtca tcccatctgc accagccgcc cctttatttc
     1261 tgactacttg aagatacgct tgattgagat tattccgatc tagaattctc ccaaacagat
     1321 tttctacacc atcttgtttc ctctgttcac cgcaagaagt gctgcgcact tcatcatacc
     1381 cttcgtgttc cacactagct ctttgatgat agccaagttt tcttgttttc tgcggtcgtc
     1441 gcatcttctc acacctccta aattattcaa gattcttatt gttcagtcct tcgtaacggt
     1501 cagtgtcgtt actactatga cctcggctga cttctcatga ttcaccatca tgtcgccatg
     1561 atcgttgtgc tggtttgttt tcattcaacg cccacaacac cctcatgaga tctcccccgg
     1621 taagagtgaa aactttcctc ccatgtaact gctagattta ctgtataggt ttcgggcagt
     1681 attggacttt gtcttgtgat gaagacttat ccgacctaat tcagccttgt atctagtttc
     1741 tgttcgtcag ttcaggagtt tgcgtccgac ttccttcagc cacaacctca cgattgccgc
     1801 cttgtctttc gctaacagtt cctactgcca agcctgtagt ggactttcac caccaagttt
     1861 tcacccatgc agggcgcaca ccaaaaaaga agcagatccg ttagcggttc tgcttctttt
     1921 agtttattat attttcttat caataatagg aacctgattt taaaagcggc tacctcttta
     1981 ccgtgcctct ttacatacaa aaaatagagg aattaagcgt aaatggcttg gctattataa
     2041 ctaaaatgct ggttatggag atgttgcagc aagttttcga gaaaagcgta ttccatctct
     2101 tgtccattga ataagatctg gatacgataa tgatttttga aacgcttctg ttcatgaatc
     2161 tgactgattg aaagttcgcc tttgtgttct gtctgataaa gaaaagtcct tttacggcat
     2221 tcagcacagc gcttggttgc tcggcggatt tttcggaatg tataaaaact agtatcttta
     2281 ttgcataaat aaacgtcttt gatcgacagc aaatcattat atagacagga acgttcttca
     2341 gctgttaatt ctgataaaaa aagctgacct tgttttattg catcttccat cggcaagtgc
     2401 ctcctcgaac ccattatcca aatttttagt taattctaat ataccatcat taagtataaa
     2461 tgtaaacgct tgttgatggt ataagatttt cgtttctgat aaaacacgaa cattaaaaga
     2521 atctaatgcc ttaaatgtac atatttttgt tgatttataa aaagatgatt gctaaaattt
     2581 aaagtgaaaa aacgaacaaa aaacgatgta tttgctttta atgtaaataa aaaggagaga
     2641 cgtacatgga gaagaaagaa cttttgtatg aaggaaaagc caaaaaaata tatcttactt
     2701 ctgatgagaa cttgatttgg atagaatatt tagatcaagc aactgcgttg aatggcgcga
     2761 ggaaagacca gataagtgga aaaggagaat tgaataatca aatcacaagt gcaattttcc
     2821 gttatctgac tcaaagagga ataaaaaatc attttattga acaattgtct aaaaccgaac
     2881 aattagcaaa aaaagtacaa attatccctc tggaagtggt tatccgaaat attgcagcag
     2941 gaagtttctc aaaacgtcta ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg
     3001 aattctacta taaggatgac cagttggatg atccatttat caatgaagag catatctatt
     3061 tcttacaact tgcaaagccg gaagaaatca gtgagctgaa agcccaagcg ttggctatta
     3121 atgaaatttt gaccgaatta tttgctaagg tcggtttggt tttagtagat ttcaagctag
     3181 aatttggccg gtcgaatgga caaattttgc ttgcggatga agtaacaccg gatacttgcc
     3241 gattatggga tgaacgtacg aatgaacata tggacaaaga cgtttaccgc agagatctag
     3301 gtgatttgat cccagtctat caagaagtat ataaccgtct atcggaacta actgaagaaa
     3361 aggagcgagt atgatgtatt tcgtaaaagt atatgtaacc tataaagatt cagtgttaga
     3421 tcctcaaggg gaggcagtaa aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga
     3481 tatccgaata ggaaaatact ttgagataca acttgctgat gcagaagaac caatcgatac
     3541 agtgattgaa acgatttgtg acaagctgct tgcaaatgta aatatggaaa cataccgata
     3601 tgaaatcaaa aaaatggagg aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt
     3661 caaattgtga cgcagatatg ttatgggcag ttcgcgagat catgggcgct gatgccgaat
     3721 ttgtacggca tgatgagtcc tctttagaag gatttgatgg ggttttactg ccgggaggat
     3781 tctcctatgg tgactatttg agatgcggtg cgattgctcg gttttctaca atcatggaag
     3841 aagtgattcg ttttgctgaa gaagggaaac cagtattcgg tacatgcaat ggattccaga
     3901 tattgacaga agcaggactt ttgccaggtg tactgagacg aaatgagtct ttgcatttta
     3961 tttgcaaaac tgtaccattg aaagtcaacc ccaagacggc ctttacttca gagtatgaag
     4021 ccgacgaaat aatctatctt ccagttgctc atggggaagg aaattatttc tgtgacgaag
     4081 aaacacttca agaactaaaa aaaaataatc agattgcctt tacttataat gacaacatca
     4141 acggcagcgt ggaaaacatt gcagggatta cgaacaaagc agggaatgtg ttaggaatga
     4201 tgcctcatcc agaacgtgca gtagaagact tgctaggatc agaagatggg aaacggttct
     4261 ttgcttccat tttaaaaaac ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac
     4321 cgacagctga agagatcaag aaaaaaagaa tctatgccga atggggattg actgatgaag
     4381 aatatcgaat gatcgaagaa gatatcttag gaagaatgcc taattatacg gaaactggac
     4441 tgttttctgt tatgtggagc gaacattgtt cctataaaaa ttctaaaccg gttctacgca
     4501 aatttcctac tagtggacct catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg
     4561 acatcggtga tggactagca gttgtcttca aagcagaaag tcacaatcat ccttcagccg
     4621 tggagcctta tgaaggagct gcgacaggag ttggcggaat catcagagat attttcagca
     4681 tgggggctcg tccaattgcg ttgctagatt cattgaggtt cggcgaatta gataatccaa
     4741 gaacgaaata tttactagaa gaagtagttg caggtatcgg cggatacggc aattgtatcg
     4801 gcataccgac tgtaggcggc gaaattgcat ttgatccttg ttatgaagga aacccattag
     4861 tcaatgcaat gtgcgtaggt ttgattgaac acaaacatat ccagaaaggg caagccgctg
     4921 gcgtcggtaa ttcgatcatg tatgtaggtg caaaaacagg tcgcgacggt atccacggag
     4981 caacttttgc ctcagaagaa tttaatcaag aagaagaaca gcaacgttct gctgtacaag
     5041 taggcgatcc ttttatggaa aagctgctgc ttgaagcgtg tttggaatta atcttgcatc
     5101 attcggattc tctaataggt atacaggata tgggagcagc aggacttgtt tcatctagtg
     5161 cggaaatggc ttcaaaagct ggctcaggac ttatattgac attagatgaa gtcccacaaa
     5221 gagaaactgg gatgacacct tatgaaatga tgttatcgga atcacaagaa cggatgttga
     5281 tttgtgttaa atcaggagag gaagaaaaaa tccaagaact atttcagaag tatgatctag
     5341 atgcagtcac tatcggtaaa gtaacagacg acgggcaata tcggctatat catcaaggag
     5401 aagaagtagc aaatctgcca gtcgatgcat tagctgaaga tgcaccagtt tatcataaag
     5461 aaatgaaaga accagctcga atcgtagaat ttcagcagtt agctccttat cagccagtta
     5521 tcgaagaacc tggacaagta ttgcttgatc tgctgcagca accgacgatt gcttcaaaac
     5581 gttcaatcta cgaaacttat gattcacaag tacaaaccaa tacagttgtc cgtccaggaa
     5641 gcgatgctgc tgtgatgagg gttagaggga cgaataaagc gttagctatg accacagact
     5701 gcaacgcgcg ctatctgtat ttggatccaa aaatcggagg acaaatagca gtggcagaag
     5761 ctgctagaaa tattattgct agcggtggta agcccttggc gatcaccgac tgcctgaact
     5821 atggttctcc tgataaacca gaagtttttt gggaattatc gacttcagct gacggaattg
     5881 ctgctgcgtg tgaaacgtta ggaactcctg ttatttcagg aaatgtctct ttatataatg
     5941 aaacggatgg tcaagcaatc tatccaacac caatgattgg tatggtcgga ttgattgagg
     6001 atcataaaca tatcactact caggaattca aaaaatcagg ggatctaatc tatatattag
     6061 gtaagacgtt tgcagatttt gatggcagtg aattacaaaa gatgcagctt ggaagaatcg
     6121 aaggtgtgat ccgaaacttt gatctatcca ttgaaaagag aaaccaagag ttagtattga
     6181 cagccatcca aaacggattg attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa
     6241 ttgcactggc agaatcagct ttcaaacatc agctagggat atctgtacag tttgaattgt
     6301 cctctgctca attatttgca gaaactcaat caagatttgt gttgacagtc gcaccggaaa
     6361 ataagacacg attcgaagaa atgatgggag atgctgctgt tttagcaggt aaggtgacgg
     6421 atgaagcgat aatcgaaatt tcagcaactg acggtcagat caaaatagaa actgcagtag
     6481 caagaaagtg ctgggaggat gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga
     6541 atgcgggatc ttcggtgttt ggggtcatcc agacgccgct agtgtggcat acttcgggct
     6601 ccatagtctt cagcatcgag gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa
     6661 gcttaagggg catcgagatt tggggctgct ttctgaggta ttcaaagatc aaagaaaatt
     6721 gcagcagtta acaggagaag ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg
     6781 aagtgtggac aatattcagc cttttctatt caaatttcat gatcagtcga tcggcttagc
     6841 gcataatggt aacttgatca atgctcgttc ccttagaaaa gagttagagg aaaatggggc
     6901 gatcttccat tctaactccg atacggagat attgatgcat ttgatccgac gaagtgaaga
     6961 aacagatttt ttaaataaat taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta
     7021 tttattaatg acagaaacgg caatgattgc cgctttggac ccaaatggtt ttcgtccatt
     7081 agcaatcgga caaatgaaaa acggagcata cgtgattgcc tcggaaactt gtgcattaga
     7141 agttattggg gcacgtttta taagagatgt ccagcctggt gagatagtga tcatcaatga
     7201 cgaaggaatc cagattgatt gctttacgaa ggacacgcag ctttctattt gttcgatgga
     7261 atatatctat tttgccagac cagattcaaa catcgcaggg atcaatgtgc atactgcacg
     7321 gaaaaatatg ggacggaatt tagcgaaaga atcgccagta aaagcagata tggtcgttgg
     7381 cgtacctaat tcttctttgt cagccgcaag cggttatgcg gaagctagtg gaatccctta
     7441 tgaaatcgga ttagtaaaaa atcaatacat tgcccgaaca tttatccagc caacgcctga
     7501 gctaagagag caaggtgttc gaatgaaatt atctgctgta cgaggagtag tggaaggtaa
     7561 aaaggtgatt atggtcgatg attcaattgt tcggggtacg acaagcagac ggatcgtcca
     7621 tcttttgaaa gaagcagggg caaaagaagt ccatgtgagg attgcttctc ctcctttgaa
     7681 atatccatgt ttttacggga ttgatattca aacgcgtaaa gaactcattg cggcaaatca
     7741 cacgattgat gaaatcaaag aatgtatcgg tgcagattct ttaggttttt taagtgaaga
     7801 gggtctgatc gaatcgatcg gtctggaaaa agatgctccg tattcaggat tgtgtatggc
     7861 ttatttcaac ggagattatc cgacgccgtt atatgactat gaagaaaaat atcaggaatc
     7921 actaaaagaa aaagtatcgt tcttttaaaa gcagaccaag aagggagtat atgaattcaa
     7981 tggcaaatgc atacgcaaaa gctggcgtag atgtcgaagc tggatatgaa gtagtagaac
     8041 ggatcaagaa gcatgtcaaa aaaacggaac gtttaggtat gatgggcgct ataggaggat
     8101 tcggcggttg ttttgattta tcggtctttc aattaaaaga gccagtcctt gtctcgggga
     8161 ccgatggcgt ggggacgaaa ttagcagtgg caatcgaagc agacaaacac gatacaatcg
     8221 ggatcgactg cgtagcgatg tgtgtcaatg atatcttagc acaaggcgca gagccgttat
     8281 atttcttaga ctacttagca acggggaaaa atgatccgtt acgtttggaa aatgtagtaa
     8341 aaggtgtagc agatggatgt gttcaaggaa atatggcgct gattggcgga gaaacagctg
     8401 aaatgcctgg tatgtactca ggtaatgatt atgatctagc cggatttgct gtagggatag
     8461 ctgaaaaatc tcaattattg acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa
     8521 tagcatctag cgggattcat tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc
     8581 agcagcagtt ttctttatca tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat
     8641 tgcttgagcc aacgaaaatc tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa
     8701 taaaaggagc tgcacatatc actggtggag ggtttgtaga aaatatcccg cgaatgttac
     8761 cagaatctat ggctgcacaa atcaaacttg gctcatggcc gattcttcca atattcggct
     8821 gtttacaaaa atatggtgaa attcctgaga tggagatgta tgaaatcttc aatatgggca
     8881 tcggtatggt cctggcagtc caagaatcga aagtaaaaga aatagtacac gaaattgagc
     8941 aagctggcga aaaagtttat gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg
     9001 tgtttatagg agaagaacga tgagaatcgc tgtttttgct tctggaaacg gaagcaactt
     9061 tcaagcgcta gccgattatc tatcgaaaaa aggattggaa tcttcgattg actggttgtt
     9121 ttgtgatcag cctgaagcat atgttttgaa acgtgcaact gcgttgagtg taccggcaga
     9181 ttgtttttca cctaaagaat ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa
     9241 attgaaagaa aaaaagatcg atttgatcgt attagctgga tatatgcgaa tcattggacc
     9301 ggtattgctg aaaaattatg ataaaagaat catcaatatc catccgtctc ttttacctgc
     9361 cttccctggt ttgcatggca tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt
     9421 cacaattcat tatatcgacc aaggtgtaga tactggacca atcatccggc aggaaaaagt
     9481 gagaattgaa caggaggaca cgttcgattc tttagaagaa aaaatccata gagtagagca
     9541 tcgaatctat ccagaagtga ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca
     9601 agagcattga tcagtgtatc agataaaact gggattgtag cattcgccca aggtttgatt
     9661 gaacaaggag tagaaatcat ttcaacagga ggaacaaagc atgcgttgga taaagctggg
     9721 atcaaaacgc tttcgatcga agaagtgaca gggtttcctg aaatgatgga tggccgtgtc
     9781 aaaacacttc atcctaaaat ccatggtggt cttttaggaa gacgcgatct ccctagccat
     9841 atggaggcaa tggaaacgga aaatatccaa ccaatcgact ttgtttgtgt caatttatat
     9901 cctttcaaag aaacgattat gaaaccagat gtcgaaatag cagaagctat tgaaaacatt
     9961 gatatcggtg gtcctagtat gctccgtagt gcagcaaaaa accatgaatt tgtcacctct
    10021 gttgttgatc catctgacta tgaagaagta ctttcagaat tacgtcagta tgggcatact
    10081 tcactttctt tcagacaaaa actagcggca aaagtctttc gtcatactgc ggcatatgat
    10141 gcactgatcg ctcaatactt aacggattgg gtggaagaaa aagaaccgga aaaattgaca
    10201 cttacgtatg aacgaaaaca aacgcttcgt tacggtgaaa atagccatca agccgctgcc
    10261 ttttatcagt ccgctcttct ggaaaaatat tcgatcgcag cagcaaaaca actccatggg
    10321 aaagaacttt cttataataa tatccgagat gcagatgctg cgctacggat catgaaggaa
    10381 tatgacgagc caacggtagt cgccttgaaa cacatgaatc cttgtggtat tgggacagca
    10441 tctacgattt tagaagcgtg ggaagcagcg tatgaagcag atcctgtttc aatctttggt
    10501 gggatcatcg ttttgaatcg agaagtagat ctgccaacag cagaacagat gcataagctg
    10561 tttctagaaa tcatcattgc cccttcttat acacaagaag ctttggaatt actgcaaaaa
    10621 aagaaaaaca tccgattact gacagtagac tttgcgcagg aagaaaatca aaatcgagaa
    10681 gaaacggtct cagtcttagg tggacttctt gtacaagatc aagatctggc aatagaaaat
    10741 gaagaagagt ggaaagtcgt gacaaaacgt aagccgactc cagcagaagc aaaagcatta
    10801 gcgtttgctt ggagagctgt caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa
    10861 cgtcagacag taggaatcgg agccgggcag atgaatcgag tcggttcagt aaaaattgcg
    10921 attgaacaag cgcaagagaa aatggaaggt gcagttttag ctagcgatgc ttattttcca
    10981 atggatgatt cggttgaata tgcagccaaa catggtataa aagcaatcat tcagccagga
    11041 ggaagcatca aagaccaaga ttctattgat atggcgaata aatacgatct agcaatggtc
    11101 tttactgatg ttcggcattt tagacattag agaggagtcg gataaatgaa cattttggtg
    11161 atcggtgcag gtggtcgtga acatgcaata agtaaaaaac ttttagccag tccgaaggtg
    11221 gatacggtgt attgtgcgcc aggaaatccg gggatgacaa aagatggtat caattgtgtc
    11281 gctatcagtg agcgagatca ttcagcgtta atccattttg ttaagaaaga ggctattgat
    11341 tggagtatcg ttggtcctga agttcctttg ttaaatggga tcgtcgatga ttttatggcg
    11401 gaaggattga aaatcttcgg tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt
    11461 tttgctaaac aattaatgga tacttatcaa atcccgacag ctaaatccag aagttttgaa
    11521 tgttacgaaa aagcagcagc gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg
    11581 gacggcttag cagctgggaa aggtgtagtc gtggctacca cagtgaagga agcgctggat
    11641 gcggcagaac aaatgttgaa ccagcatcga tttggggata gtagcaaaaa aatcgtcgta
    11701 gaagagtttt tggcaggaga agaattctcc cttttggcgt ttgtccgtaa taccaaggtg
    11761 tatccgatgg tcatttcgca agatcataag cgggcatttg ataacgataa agggccaaat
    11821 acaggcggaa tgggtgctta ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa
    11881 gctgtggaag aagttttaaa accagcggct ttgggaatga aagaactagg acgctctttc
    11941 acgggtgttt tatatgcggg tttgattgct acgaaggaag gaccaaaagt gatcgaattc
    12001 aatgcgcgtt tcggtgatcc tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt
    12061 gcgcaagtaa tcgatgattt attagagaat cgtacagtag agttaaagtg gcagcaagaa
    12121 ggatacaatg taggcgttgt ggttgcagct gccggttacc ccgaagaata tgagacagga
    12181 atggttctac cagattttct ggaagaagag ctttctgttt actatgctgg agtttcctca
    12241 aataacggca aactagttgg agcaggagga cgtctttatt tagtagaagc atacggtgag
    12301 acgatcaaag aagcacagaa aaaaatttac accgcgttaa tggataaaac gacagatgga
    12361 acattttatc gtacagatat tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa
    12421 atcagctgct tccatttata aaaaatacaa caaacataat cggaagcgcc gattttagtg
    12481 aagccttttc ataaagagtg tggtacactt ttgttgtgta tataaataag gaggagttca
    12541 gatgaaaaaa attcaattag gtacttctga tcagaaagca gctgcagtga tcttaggctg
    12601 tatgcgtatc aacaatgcgg aagatccagt aaaagtgatt gagacagctt atgaaaacgg
    12661 cattgatttt tttgaccatg cagatattta tggaaacggt gaatgtgaag aaatctttgc
    12721 agatgctcta gcaaaaacat caatcaaacg agaagatctt tttattcaaa caaaatgtgg
    12781 aatcgttcca ggtgtgatgt ttgatttttc taaaaaacat atcattgaat ctgtagaaaa
    12841 aagtttgacc cgtctgaaaa tggattatgt ggatgcttta cttcttcatc gtccagatac
    12901 attggtagaa ccagaggaag tagctgaagc gtttgatgaa ttggaacggt caggaaaagt
    12961 tcgttatttt ggcgtcagca atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt
    13021 tcgtcaacca ttagtagcta atcagctgca atttggcttg aaacatactg gaatgatcga
    13081 tcaagggatt catgtcaata tgacagatga agcaagtatc gatcatgacg gtagtgtact
    13141 tgattattcc cgattgcatg atatgacgat ccaagcatgg tcaccatacc agtatggatt
    13201 ttttgaaggt gtatttatcg ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga
    13261 actagcagct aaatatcaaa cgacaccaac tggcttggct tctgcttgga ttttacgcca
    13321 tccagccaat atgcaagtga tcgccggtac gatgaacgta gggcgaatca aggaaatcgc
    13381 tcaagcttca gaaattaata tgtctcggga agattggtac gaattgtacc gtgctgctgg
    13441 aaacatttta ccttaaagat tgtcataagc aggtcaaaag ggctataaat taaaaagaca
    13501 gaataacttg aaagctgttt tcagcttcga gatattctgt ctttttttat tgcaaatgga
    13561 aagagtatca ttgtacggat ttcgtttatt agttattttt gtttttaagg aaaagctgtg
    13621 tccgatattc ttttggagaa atcccttgtt ttttcttaaa taaataactg aaatagttag
    13681 ggtcattata accaatttca aaagcaatat ctgaaatacg atagtctgat tgctgcaata
    13741 atttttttgc taattcgatt ctttgttctg tcagataatc gatgaatgtt tggttaaacg
    13801 attgagaaaa aatcgtactg aaatgagagg gacttaatgc aacttgttga gcgacccaag
    13861 ttaaggacat atctggatcg gtaaaatttt ctttgataaa atccaatgca tggttcaaaa
    13921 ttggctgata tttgttcctt gaaggctgtg agggcagagc agttaagaaa gaaatgaaca
    13981 tataaactgt cgaacggtaa agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg
    14041 atgtgcaatg aaccatttct tgcgggattt tcttttcttt caggcagaca tgctggagta
    14101 gcttagtcaa gacaaataaa cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg
    14161 tcaagtgttc gatgtactta gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga
    14221 ggtcaaagta aaagacagaa tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc
    14281 tgataatttg ttctttaagg gaactggcgg tttcaagtat catctgcgtg atctcaaaag
    14341 atgcaggtaa ttcgctgatt ctttctgcga caggaccaat ggcgacagaa aaattatttt
    14401 tttgtccctt ttctaattca tggaccaacg tttgggctac ctgataactg gtctcgatca
    14461 cattttcttc aagagaatca actattaaaa atttgatgaa tcgagaggaa acagcagaga
    14521 agatcacttg ttgattctcc ctaaataaaa acttcaaata atcactgaga tgagcgtaat
    14581 cttcaaagtc tgtactgact tggtttgctg ctaataatac ggtcattttt ttccctgcta
    14641 aagagcgatt gaaagattca gcaagtttca atgtttcact gatcgtaagt gagcctttga
    14701 acaaaccgtt caagaaatga ttttttttca attcgaaaac aagatcagaa gaagtgtgcg
    14761 tttctttttg ctgcaaatct aaagcagtta ctgctttttc tattgcttgt ttcaattcgc
    14821 tacttttaat cggtttaagc agataatcat ctgcctgtac ctggattgct cctttcatat
    14881 aatcgaaatc gtcgaatcca ctaataaata tgagacgagt ccaaggaagt agtttccttg
    14941 cttcttttga aaaagtcaac ccgtccatga aaggcatttg gatatcagtc agaatgatgt
    15001 cgggctgtaa gtcgataatt gaagacagtg ccatctcacc atctccagct tcgccgacat
    15061 aagtgattgg ataataagaa ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga
    15121 ggtattcgtc ttcgatgata aaaactttat acatgtttgt ttcctccaat tgttttggga
    15181 gatagaggga tcatcagtgt gacagttgtt ccttctcggt atttgctgtc aatcgtgata
    15241 ggtttttgat catttccgta ataaagaagg agacgcttgt ttacgttgta aagtccgtag
    15301 ccgatactga aatctgaatc gatccctttg gctaactctt tacgtatttc tgccagtttt
    15361 tctgcaggga tgccaatccc gttgtctata actttgaatt ggatcatctc atcaattttg
    15421 catgtggtaa tacgaataaa tcctttgtga cggacgtact tcgttccatg gtatacagca
    15481 ttttcaatca atggctgcaa gatcattttt aaaatagaaa caggaaaaag tgtttctggt
    15541 atatcgatct tatagtcaag catatggccg tatcttattt ttaagatgac tagataatca
    15601 ttgatatggc taatttcttt ttcaagagga atccagtctt gtcctttgct taaagacgta
    15661 cgaaaaaagc cggaaagtgc atgggtcata gtcagagctt cttcaaagca gtcttgttcg
    15721 atcaaagaaa taatggcatc caaactgtta taaataaaat ggggagtaat ctgagcttgc
    15781 aatacacgaa cttcactttg tgctaaatgg tattgtttca aagcgttctc ccgtaaagac
    15841 tggtttagat cttccgccat tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt
    15901 tccggcaggg ttaaacgaaa gccaaggttc cctgaagaaa gttgttctga catctcgatc
    15961 agctgattga tcggttcttg gacctttctc tctaaaaagc gacgattctt tttcgcatag
    16021 aaaaaaatgg tactaagaac tccaagttcc actattgtta atagaatgag cgagcggaca
    16081 agtgtcgtat tttttttgct tgctaagtcg atctccacac gaacaaaatc ttgtaagata
    16141 tctgacagca gttgagtgac agaatctacc tgattgatga tctcttcatt ggtatccata
    16201 aaatcttttg ccaagatatt attgatcagc tcgttttgat aatcttccag tgtatcgatc
    16261 gtacgtagcg atacatctaa cgtactaatt tctttaagtg tatttgtatt atttttgatc
    16321 tctcgaatat cattcctaag ttcttctagg atattttcct cattgaactc atctacggtt
    16381 acttggccag aaatcaagtc ccacatactt tccaaaactt tttgatcaac cttcaaagca
    16441 attgcatctg ctttttggat atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa
    16501 tataacagta tgctcagtag caaagggata acagcaaata gaagcatggc tttgtttgct
    16561 tgggcaaaaa tctgttttat gcttaaattt tttgtcatga tacttctcca gtcaataatt
    16621 tctggttggg atgttagcta tattctgctg ggaaaagaca gtctcggaaa taaaaatatc
    16681 tttaggtaat gtttttcctg tttgaatatt tccaaaataa cgagtaacgg tattggctac
    16741 ataccagccg acatcaggtt tacactcgac tacacaattg acgattcctg agcgtagctt
    16801 ctcgatcata tcttcttgtc catcaatagt tacaatgatc aaatcttctc caggtttgat
    16861 tgatgtttct tttatcgcag ctaaagcacc gtgagtcatt tcatcactat ggctgtataa
    16921 tacatcgatt tcggataaat catttgtttc taaatatcgt ttcatctggt cttttccttt
    16981 catccgaata aaatcaccga caattcggtg aacaatagtc atcggaggtt tatggtcgat
    17041 ggttccatct ttttttaggg ttggtttacg attgattgat tccaaaaatc cttcggagcg
    17101 tagcttggtc ggtgtggagt tctctgaacc agcaagttct aggattctga tttcctgttt
    17161 ctttgaattt gtgaagtgat tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa
    17221 actggggcca atgtgggtga cgaaaagctc gggatcattc gtttgaatat gtctgtcgac
    17281 aagtatgaca gggatccctg ctcgctttgc ttcttttaat acagagtccc acccggattc
    17341 ttgcaaagga gtaaaaatga tcatatccac tttataggca atgaacgtcc gtatatcttg
    17401 gatttgccgt tcctgattca tgtagccatt tcggtagaga acttggtaat cttctttttc
    17461 aagcgcagtg ataattgatt cagtatgctt tttccgccag ctactctccg ttcctgattg
    17521 cgaaaaacca acgactagtg ggtcaggtgc ttcctttgta ttaggtgtac accccatcat
    17581 ttggtaagcg attacaacaa gggctaaaaa aaataaaaca cgtttcactt taaatcactc
    17641 catctggatt tgttagtact atcttatcag ataaaaggga aaatcaaagt caaaaggaat
    17701 caatcgaaaa aaatccaaga aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa
    17761 atttaaagta aatcccataa aaaaatcaat gttaacgctt tctttattag ctatactcgg
    17821 tgtatcaatt gaaatggggg ataaaaaatg aaaaacaaat ttagaaagat tgttgggact
    17881 atcgctattt tgaccgcaag cattctatta agtgcgtgtg gcaattcagg atctgcagat
    17941 gatagtacgg ggtatgtagg aatcgctatg ccgaccaaat cagcagaaag atggatcgct
    18001 gatgggaaca acatggtctc ggaattagaa aaactgggtt ataaaacaga tcttcaatat
    18061 ggagaagata aagtagaaaa tcaagtagca caaatagaaa atatgattac aaaaggggta
    18121 gatacacttg tgattgcttc tatcgatggt tcggctttaa ctgatgtgtt agctaaagca
    18181 aaagaagcag atatcaaggt gatcgcctat gatcgtttac tgatgaattc agaaaatgtc
    18241 gattattacg cgacttttga taattttgga gtaggtgtat cgcaagcagc ttatattgaa
    18301 gagcacttag gattgaaaga aggaaaagga ccattcacga ttgaattatt tggtggctcg
    18361 cctgacgaca acaacgcact aatcaattac aacggagtga tgtctgtttt acaaccatat
    18421 atggacaacg gtcagttggt cgtgccatct ggacagacga gtttcagtca aatcgctacg
    18481 cttcgctggg atggttctac ggcgcaagca aggatggata atctgttaag cgcaaattat
    18541 actgaccaaa cattggatgc tgttttgtcg ccatatgatc caataagttt aggaatcatt
    18601 tcgtcattaa aaggcgtggg gtatgggagt gaaagtaaac ctttacctgt gattactgga
    18661 caagatgcga cagttgctgg cgttaaatcg atcattgccg gggaacaaac acaaacgatc
    18721 tttaaagaca cacgtatctt agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat
    18781 ggagaagagg ttccggtcaa tgatactgaa acttatgata atggggtgaa aactgtccct
    18841 acgtatttag caaatacagt atctgtcgat aaagataatt atcaagcaga gctgatcgac
    18901 accgattatt ataaagaatc cgatctgaaa aattaagagg aaccaaacaa ggaactaaag
    18961 agatctaagt cagagagagg ataagaatta tggcggattg tattttaaaa atggcagata
    19021 tcaccaaaaa attttctgga atccgagcgt tagattgcgt gaatctgatg attgaacgtg
    19081 gggaaatcca tgcattgtgc ggagaaaacg gagcgggaaa atcaacattg atgaatgtct
    19141 tgagcggctt gtatccatat ggctcctatg acggggaaat cttttatgat ggaaagctgt
    19201 gtcaatttaa agatttgaaa gatagtgaag aaaaaggcat cgtcatcatt catcaagaat
    19261 tagcactaag tccttatctt tcaataaaag agaatatttt tctcggaaat gaacaagcag
    19321 aaagaggcgt gattgattgg gaactgacag aaaagaaaac atatgaactg ctgaataaag
    19381 tcggtttgtc tatcgacccg aatcttctag tatcccagat tggcgtaggg caacagcaac
    19441 tagttgaaat agctaaagca ttatcaaaat ccgtccgtct gttgattttg gatgagccta
    19501 ccgctgcatt gaatgaagaa gaaagcgcga atttgctgag attgatccga gagttgaaag
    19561 agcaaggtgt aacctccatc attatttcac ataagctgaa tgaaatcgtg gatatcgcag
    19621 atcgaatcac cattttacgg gatggacaga cgattgagac actagaaaat gaaatgatca
    19681 gcgaagagca tattattcgc ggaatggtgg gcagagattt agttgatcgt tatcctgaaa
    19741 gaaaaccgga tattggagaa gtttatttta aagtagaaga ctggacggtc tatcaccctc
    19801 ttgatcacga gcgaatcata aacgaccaca tcaacttgca tttgaaaaaa ggagagattg
    19861 taggaatagc tggactgatg ggagcgggca gaactgaatt cgctatgagt ctatttggac
    19921 attcttatgg aagcaagata tccggacgta tattaaagga aggggcagag atatcagtta
    19981 agaccgttcc ttcagccatt gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt
    20041 taggactgaa tctgctgatg gatattcggg aaaacgtgac gctgagcagt ttggataaaa
    20101 taagtcaaaa aggagtatta gataaagaaa aagaagtaac tgaggcagaa aatttccgga
    20161 aaaagatgcg tataaaaaca aactccatcc atcaaattgt cagtagtctt agtggtggga
    20221 atcaacaaaa agtcgtttta tccaagtggc tgatgacaca acccgatatt ctttttttgg
    20281 atgagccaac ccgtgggata gatgttgggg caaaatatga gatctacacg attatcgaag
    20341 aaatggcagc aaatggcaaa gctgtctgtc ttatctcttc tgaacttcct gaaatattag
    20401 ggatgtgtga tcgaatctat acgatgaacg aaggacgtat tacaggagaa gtctcgcgag
    20461 aagatgcaga tcaggaagtc ttgatgaaat taatgacgaa agaagaggtg ggttaagttg
    20521 gaacaggcaa aaaatcttac ggataaaaaa gagaaaaaac taaatttgaa aagtagagca
    20581 gctgatcttt ttggtcgcta cagcatggtg atcattttag cgggactact tattgcgttt
    20641 caattgatga cggatggtat tttctggcgt ccattgaata taactaacct tgttctgcaa
    20701 aatagccata tattagtact agctgcaggg atgttattag ttgtgctctt aggtcatgtg
    20761 gatttgtcgg tagggtcagt gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc
    20821 aatcgccaaa tgaacccgtg gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc
    20881 ggcgcatggc aaggtttttg ggtagcttat gcagggatac cggcgtttat cgtgacttta
    20941 gcaggattgc tgatgtttcg tggactgaca caagttgttc taggaggcca atctctagca
    21001 ccttttccag ctgttttcca aaaaatgtct acgggttatt taccagactt aagcgaagga
    21061 agcagccttc acttcttgac attgatttta ggaatgatca tcacgatcgt gttagttgtt
    21121 ggtcaatggc gaacaagaaa tcgtcggaaa aatcatttat ttgaagtgga atctccaaca
    21181 gctttcttgg taaaggcggt atttacagca gtcgttgtac taggaataac ttatatcttt
    21241 gcctcttatc aagggtatcc gattattttg atcattcttg gtttgatcgt tgcagcatat
    21301 gggtttttga caaataaaac agtagcaggt cgacagattt atgcgacggg cggaaatcga
    21361 aaagcggctg aactatcagg gatcaaaaca aagcgaatca cgttctgggt attcgtcaac
    21421 atgggagtca tggcagcttt agctggatta gttttagctg ctcgattgaa tgcggcaaca
    21481 cctcaagcag gtacatcgat ggaactggat gcaatggcgg cagtatattt tggtggtgct
    21541 tcgacttccg gcggcattgg aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt
    21601 gtattgaaca atggcatgtc gattttaggc gtcggggtag actggcagca ggcaatcaaa
    21661 ggattgatct tgcttttagc cgttgtgcta gacttgtata ataagaaaaa acgaacaacg
    21721 taggagaacg ccggttattc agaaaaaaga ctgtgacaag acatttgtca cagtcttttt
    21781 ttcgctaaaa gcgaacattc attgtttcgt ttgtatagac taaaaataat atatgttttt
    21841 ttaccattcg tataaattct ctttttgctc ttgatttttt tgcttaattg agttaaatta
    21901 catagggcgc taaaaaaagc cttgattaaa ttaataatgt tcgtgttttg ctggatgtga
    21961 acatcaggag gataatgtaa tgaaagtatt tgattacgaa gatattcaat taattcctaa
    22021 taaatgtatc gtaaatagcc gttctgaatg tgatacaacg gtcacattag gtaaacatac
    22081 attcaaaatg ccagtagtgc ctgccaacat gcagacgatt attgatgaaa caatcgcaga
    22141 attcttagcg gaaaatggtt atttctatat catgcatcgg tttgatgaag cagctcgaat
    22201 tccgtttatc aaaaaaatga aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa
    22261 gaaagaggaa tattccttta tagagaagtt ggcagaagaa tcgctaaatc ctgattatat
    22321 cactatcgac atcgcgcatg gacatgctaa ctcagttatt gatatgattc agcatattaa
    22381 aaaatatttg cctgaaactt ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg
    22441 tgaattggaa aatgcgggcg cagatgcaac aaaagtcggg attggtccag gaaaagtatg
    22501 tattacaaaa atcaagacag gctttgggac tggaggctgg caactggcag ctcttagatg
    22561 gtgtgcgaaa gcagctcgca agccgatcat cgctgacggt gggatcagaa cacatggcga
    22621 tattgcaaaa tctgtccgtt ttggtgcgac aatggtcatg attggttcat tgtttgccgg
    22681 tcacgaggaa tctcctggcg aaacaaaagt cgaaaatgga atcgtgtata aagaatactt
    22741 tggttctgct tcagaattcc aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg
    22801 gatccaacac aaaggatctt tgaaagatac actagtggaa atgcaacaag accttcaatc
    22861 atctatttct tatgctggag gaagagatct ggaagccatt cgaaaagttg actatgtcat
    22921 cgtgaaaaat tcgattttta atggagacac aatctaaaaa ataaataaaa tattaggatg
    22981 ttgtgaaaaa gttgtcctgc attaagtaat aagaacagcc aaacaaaaat agttctttat
    23041 atgttttgtt tggctggaat tattatcgca gatacaagct tttgaacatc attattcaga
    23101 ataagggact gtgataagat ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc
    23161 ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt
    23221 atgtgggaaa aagtagagaa aaaacaagga ccgatttatc gtcaaattat ggaacagatt
    23281 atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc aacttgaatc agaacgaaaa
    23341 ctagggcaac tttttggtgt gaatcgttcc accgttgtcc atgcattgga agagcttagg
    23401 gagcttgggg tactaacgag caaacgtggc agcggatggt ttgttaatca gactgaatgg
    23461 gggaaattcg ctgttccaag aatcgattgg cggcaaatga tctctcctcg ttatgagcag
    23521 acggactggt atgagcaaaa aataagagaa gcaaagcaga tccaaaaaac atcgtttctt
    23581 gatttatacg ctagcgagat gccagatgag cttttgccga attttgagtt tccttcttat
    23641 tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc ttttaggcta tactccgtta
    23701 cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat tggcattttc ccccaaccag
    23761 cttcttgtga ctggtggcgg tcagcaagca atctttttga ttttacaaac gattctttca
    23821 gctggagagg ggatagctgt agagtcacct tctttttttt accgattacc tcttttcaaa
    23881 gcttcaggaa cgcgtttatt tgggataccg atggatgaag aagggatcga cttagaaaaa
    23941 ctgaaagaat cgatccataa aaataaattg aaagcagtgc ttatcaaccc gaattttcaa
    24001 aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga
    24061 cagtatcggc ttccaattat tgaagacgat gtatttacgg atctttcatt tctaaaccaa
    24121 gaagaagtcc ttcctatacg aacgattgat ccggataatg tgttatacgt cggttcgtta
    24181 tctagggttt taggaaaaac aacgaaaatc ggttgggtga taggacctga gacttttatc
    24241 tcgcaactag ctcaagcgca agaaatgatg gagttttcga tgaatatcct tacacaaatc
    24301 acagtggcta atgtatttaa tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag
    24361 ttgaaagaaa aaagccgact gataagcgct tggggagatt cacaatcgtt tttcaagctt
    24421 cattcaattt taggtggtta ctatgcctgg attacttggg atgggaaaaa actagacccc
    24481 gaactagcag aaaaagtagt agatatgggt cttggaattg caccaagtct actttttggg
    24541 caagatataa atggaatcag aatcaattac agtaggctgg atgaaaacca aataccgttg
    24601 ttttattcaa agatgaaaat tctagaaaag tggttaacag aataatttca caaagtaaat
    24661 agattaaaga atataacagc ttggatttaa atttcaagct gtttcttttt tttcggaaaa
    24721 agaatacgat ataaagaaac taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt
    24781 aaataaaata aatgtttgca acaaaactgt ataacaccaa cgtttttaaa tagaaacaga
    24841 tggttgtaag tttgattaga aaatagaaaa aatgcagatg aaatataaaa cgctttcctt
    24901 attgtgattt tattcactaa tgctgttaat atgcggattg gcataatttc ctttcatcgt
    24961 gaaaatttgt gcttttagct tgtatttctc ataaatccat gataatttat taatgaagac
    25021 ggttaaatag tgatacagtt ttattcactg tttcaatcgc agaatgaaga gattttctta
    25081 tagatgagag aggaatgtgt gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc
    25141 actgctagat gaagtcaatg cagatttccc agtatatcaa gcacttgatc aagatggaaa
    25201 cgtagttaac aaagatttag tacctgattt atccgatgat gaactcgttg aattaatgac
    25261 tcatatggta tggtctcgtg tattagacca acgttcaaca gcgctgaacc gccaaggacg
    25321 cctaggcttt tttgcaccaa ctgctggaca agaggcaagt cagctggcta gtgcttacgc
    25381 atttgataaa gaagatgttc ttcttccagg ttatcgggat gtacctcaac tagtaaaaca
    25441 tggtttacct ttatctcaag cttttttatg gtctcgagga catgctgcag gtaattctta
    25501 tccggaagaa ttgaaagctc taccaccaca aattatcatc ggtgcacaat atgtacaagc
    25561 agccggtgtt gcattaggtt tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac
    25621 tggtgatggc ggttcatcac aaggtgattt ttacgaagca atcaatttcg ctggcgctta
    25681 ccatgcaaat gccgtatttt atatccaaaa caacgggtat gcgatctcta cacctcgtga
    25741 aaaacaaact gcagcaaaaa cacttgcaca aaaagctgtc gcagctggga ttcctggtat
    25801 tcaagtcgat ggaatggatc cgcttgcagt atatactgta tctaaaatgg cacgggaatg
    25861 ggcgatcagt ggaaatggac cagtcctaat cgaaacactg acttatcgtt atggaccaca
    25921 tacactatct ggtgacgatc caacccgcta ccgtacaaaa gacacagatg acgattggca
    25981 gaaaaaagat ccattgatcc gcttccgtaa atatttgacg gaaaaaggac tttggtctga
    26041 agaaaaagaa gaacaagtca tcgaagcaac taaagaagaa atcaaagcag ctattgctga
    26101 agccgacaaa gtaccaaaac aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc
    26161 tccacaatca atcaaagaac aaattgcaat ttatgaagcg aaggagtcga aataatcatg
    26221 gcacaaaaaa caatgatcca agcaatcaca gatgccttag cacttgaact agaaaatgac
    26281 gagaatgtcg ttgtttttgg tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact
    26341 gaaggattgc aggaaaagtt cggagaagac cgagtattcg atactccttt agctgaatct
    26401 ggaatcgcag gattgagttt cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc
    26461 caattctttg gttttatttt cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca
    26521 agatatcgaa tgagcggcac tagaaatcta cccatcacaa ttcgttcgcc atttggtggc
    26581 ggtgttcata caccagaatt gcattcggat aatttagaag gtttgatcgc tcagtctcct
    26641 ggtattcgag tagtgattcc ttccaaccct tatgatgcaa aaggactatt gatttcttct
    26701 attagaagta acgatccggt tgttttccta gaacacatga aactatatcg ctcattccga
    26761 gaagaagtgc cggatgaagc ttacgaagtt cctttggata aagcagcagt tactcgtgag
    26821 ggaacagatg tatctattat cacatacgga gcaatggtgc gtgaagcaat caaagcagca
    26881 gacaatcttg caaaagaaaa tatatctgtt gaaatcattg atcttcgtac agttgcgcca
    26941 ttagatgtag aaacgatcat tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa
    27001 gaagcacaac gacaagcggg cgttgccgcg caagtagttt ctgagatctc agaacgcgct
    27061 atcctttcat tagaagcccc aatcggacga gtttctgcac ctgatacagt cttcccattt
    27121 ggacaagcag aaaatgtctg gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa
    27181 atcgcggaat tttaagaaaa gaagggacga aacaaagtca tggcttatca atttaaattg
    27241 ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga
    27301 gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa
    27361 atcccgtcac ctgttacagg aacggttaaa aatgtgattg ttccagaagg taccgtagca
    27421 aatgttgggg atgtattagt agaaatcgat gcaccaggac acgaagacaa tgaaggggac
    27481 agtggtgtag ccgcagaatc ccaaactcca gctaaaccgg ctgctgaacc cactgttgat
    27541 acagaatcag ctggatcgtc aagcgaagga gtcttccaat ttaagttacc ggatatcggt
    27601 gaaggaatcg cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat
    27661 gaagatgata cattactaga agttcaaaat gacaaatcag tagaagaaat tccgtcacct
    27721 gtcacaggga cagttaaaaa cgtgattgtt ccagaaggca ctgtagcaaa tgttggggat
    27781 gtattagtag aaatcgatgc tcctggacac aatagtgcac cgtcaactag tgcgccatca
    27841 gctgaagcac caaaagaaaa agtggaaaca tcaggttcag caagtgtcgt tgaagctgcc
    27901 gatccaaata aacgtgtatt ggctatgcca tctgttcgtc aatttgcccg tgaaaaagat
    27961 gtagatatct cacaagtaac tgcaactgga aaaggtggca gagtaacaaa agaggacatc
    28021 gaaaacttct tggcaggtgg tccaagcagt gcaccagcga aatcagaagc gccagaagcg
    28081 gcagcaccga aagaagcagc tcctgcagca gaaagcaaac cagctgcacc agcaaaacca
    28141 ttcaagtcca acttaggaga cttggaagaa cgtgtagcaa tgacgccaac acgtaaagct
    28201 atcgctaaag caatggtcaa cagtaagcat acagctccac acgtgacatt gcatgatgaa
    28261 gtagaagtgt ctaaactatg ggataatcgg aaacgattta aagaagttgc tgctgcaaat
    28321 ggtacgaaac ttactttctt gccatatgtc gtgaaagctt taacagcgac tgttaagaaa
    28381 tacccagtat tgaatgcatc tatcgacgat gcaaaccaag aaatcgttta taaacattac
    28441 tacaatatcg gtattgcaac tgatacggat cacggactat atgtaccaaa tgtgaaagat
    28501 gcagaccgca aaggaatgtt tgctattgca gacgaaatca atgagaaagc aaaattagct
    28561 catgacggca aactatctgc agaagatatg cgcaatggaa caattacgat cagcaatatc
    28621 ggatctgtag gtggcggttg gtttacacca gtcatcaact atccagaagt agctatttta
    28681 ggtgtaggaa cgattgcaca acaaccaatc gtaaatgctg aaggcgaaat cgttgtcggt
    28741 cgtgtgatga aactgtcatt aagtttcgat caccgtatcg tagatggagc aactgcgcaa
    28801 caagcaatga ataatatcaa acgtctttta gctgatccag agctattaat gatggaagga
    28861 tgataaacaa atggtagttg gagattttgc tgtagaatta gatacagttg taattggtgc
    28921 gggacctggt ggctatgtag ctgccatccg cgctgctgaa atgggacaaa aagtagcaat
    28981 cattgaaaga gagtatattg gcggtgtttg tttgaatgtc ggctgtattc cttcaaaagc
    29041 actgatagct gcaggacacc attatcaaga gtcactagat tctgaaatgt ttggagtaac
    29101 tagtgaaaac gtcaaactag attttgcaaa aacacaagaa tggaaagaaa acaaagttgt
    29161 ccatacgttg acttctggtg ttggtttcct attaaaaaaa cataaggttg aaacaatcga
    29221 aggagaagcg ttcttcgtag atgatcacac actacgcgtc attcatccag attcagctca
    29281 aacttattcg ttcaataacg caatcatcgc aacaggttcc cgtccaatcg agattccagg
    29341 atttaaattc ggaggacgtg tgttagattc tactggcgga ttagcattga aagaagtacc
    29401 taagaaattt gtgatcatcg gtggcggtgt gattggtgcc gaattgggcg gagcgtacgc
    29461 aaacttagga gcagaagtaa cgattcttga aggctcacca caaattttac caacttacga
    29521 aaaagatcta gtaaaattag tagaagatga tttcaagaaa aaaggcgtaa cagtcgtaac
    29581 gaatgcaatg gcgaaagaag cagtcgataa cggtgacagc gtgacagtga aatatgcagt
    29641 ggacgggaaa gaagaatccg ttactgctga ttatgtgatg gtcactgtag gtcgtcgtcc
    29701 gaatacagat gatatgggct tagaacaagc gggtgtcgaa gtaggagaac gaggattgat
    29761 cacggtggat aaacaaggac gtacgaacgt acctaacatc tatgccatcg gtgatatcgt
    29821 tccaggggcg gcattagcac acaaagccag ttatgaagca aaaatcgctg ctgaagcaat
    29881 ctctggcaaa aaagtagctg tagattataa agcaatgccg gcagtagcct tcactgatcc
    29941 tgaattggct tctgtaggta tgacgatcaa agaagcaaaa gatgctggta tcgaagcaaa
    30001 agcatataaa ttcccatttt cagggaacgg tcgggcatta tcgcttggta aaacagaagg
    30061 attcatccgt ctagttacga cgatcgaaga taacgtgctt attggcgcgc aaatcggtgg
    30121 agtcggcgca agtgatatgg tctctgaatt agctttagca atcgaatcag gaatgaatgc
    30181 tgaagatatc gccttgacga ttcatcctca tccatctctt ggcgaaatcg tcatggatgc
    30241 ttcggaatta gcattaggtt taccaatcca tatttgattt gaataataca ggcgaaacgc
    30301 aactgaaaaa tcagttgcgt tttgtttttt agaattatca ataatattat tatgtaaact
    30361 taaaaatatg acaacttctt ggtttattac cacttcctat gctacactga agaggaatat
    30421 gaaataggag agttgaagat gcaagaatat cagtatccac ttgatttaga ttggacaaca
    30481 gacgaaatgg tcattgtaat gaagatgtgg gaagatctgg aacgtgcgaa tgaaaaaggg
    30541 ataaacaatg aagctttttt gaagacttat cagcagttta aaactgttgt caaatccatt
    30601 ggtgaggaaa aacgattggg acgggaattt gaaaaagctt ccggttattc tttatatcgt
    30661 acagtgcagc aagcaaaaaa aaatccaaac aaaactttga gaatgaacgg gtgagataaa
    30721 tggaacagat gattgatcat atcaagtcct ggcttgaaga agctggtgag aaaatcaaat
    30781 cttctcaaca aacattaaca atctcgcaaa aatcaaatcg aacggatcta gtaacaaatg
    30841 tcgatcgaaa catccagaat tttctaatcg aaaaaatcca ttcctttgat ccagaggcga
    30901 aaatattggc agaagaagaa gggtgtaatc aattatcgga tctcgatgga agagtcttta
    30961 tcatcgatcc tattgatggc accttgaatt ttgtgattga aggggaaaat ttttgtatta
    31021 tgctcgctgt ttatgaatcc ggcgttggaa agcttggttt catttatgac gtgatgagaa
    31081 atgaactttt ttggggtgga aaaggaatag gcgtgtataa aaatgatgag ccattgccta
    31141 gaccagcaaa taaatcctta tcggaaggct tacttggtgt gaatagttat cttttcggtc
    31201 ataataaata tggaatccga gaaattgcgg aaagcagcat ggggacacgt atgtacggat
    31261 gcgctggtct tgaactagtg gcaatcttga aaggaaacca cattgcttat atttcaaacc
    31321 ttagcccttg ggattatgca gcgggaaatg tcttattgga agagtttggt atggtaacaa
    31381 gcggtctttt tggtactcct ttgacatttg aaaaaaggga atactattta gcagggactt
    31441 cttgtgcata tgcagaaatc gcagaccttt tgaaaaaaga aggctgaaaa aaaggtagaa
    31501 atcattcaca aaaatgataa aaacgagcaa aagattaatt ttctcgaaaa aatgaaaaca
    31561 ttttactata tttcatgaaa aaagtctgct ataattagca gtcgagtaaa atttgccctg
    31621 tcataaagaa ccaataaaaa aggagttagt tacattgaat tatagaaacg atatccgtaa
    31681 cgtcgcaatt attgcccacg ttgaccacgg aaaaacaaca ttagttgatg aactattaaa
    31741 acaatcagat acattagacg cacatacgca attgcaagaa cgtgcgatgg actcaaatgc
    31801 gctagaaaaa gaacgtggta tcacgatttt agccaaaaac acagctgtag actataaagg
    31861 tattcgcgta aacatcatgg atactccagg acacgcggac ttcggtggcg aagtagaacg
    31921 gatcatgaaa atggttgacg gcgttgtgtt ggtcgtagat gcgtatgaag gaacaatgcc
    31981 tcaaacacgt tttgtattga aaaaagcact ggaacaacat atcacaccaa tcgttgtcgt
    32041 aaacaagatt gacaaacctt ctgctcgtcc tgagcatgtc gtagatgaag ttcttgaact
    32101 attcatcgaa ttaggagcag atgatgatca gttagacttt cctgtgattt atgcatctgc
    32161 actaaatggt acgtcaagct tgtcagatga tccagcagat caagaaccaa ccatggcacc
    32221 tatttttgat acaattattg aaaaaatccc tgctcctgtc gataacagcg acgaaccatt
    32281 acaattccaa gtttcattac ttgactacaa cgattatgta ggtcgtatcg gaatcggacg
    32341 tgtatttaga ggaacaatca aagtaggaga ccaagtagca ttaattaaat tagacggaac
    32401 agttaagaaa ttccgtgtaa cgaaattatt tggtttcttt ggactaaaac gtctagagat
    32461 tcaagaagca aaagctggag acttgattgc tgtctcaggt atggaagata tctttgtcgg
    32521 tgaaacagtt actcctgtgg atcatcaaga tgcgttacct atccttcaca ttgacgaacc
    32581 aacattgcaa atgactttct tagttaataa ttcaccattt gccggtcgag aaggtaaatt
    32641 cgtaaccgct cgtaagattg aagaacgttt gatggcagaa cttcaaacag atgtatcctt
    32701 acgagttgaa ccaacaaact ctccagatgc atggacagtt tctggtcgtg gagaattgca
    32761 tttgtctatc ttgattgaaa acatgcgccg tgagggatac gaattgcaag tatctcgtcc
    32821 agaagttatt gaaaaagaaa tcgacggtgt aaaatgcgaa ccgtttgaac gcgtgcaaat
    32881 cgatacacca gaagaatata tgggtagtgt tatcgagtcc ttgagcttac gtaaaggtga
    32941 aatgcaagat atggtccata caggaaacgg acaaatccgt ttgacattct taacacctgc
    33001 ccgcggtttg attggttatt caactgaatt cctttcaatg acacgtggtt acggaatcat
    33061 gaaccatact tttgaccaat atctgccaat gcttcctggt caaattggcg gacgtcacca
    33121 aggggcgctt gtttcgatcg atacaggaaa agcaacgact tattctatca tgagtatcga
    33181 agaacgcggt acagtcttcg tagaaccagg tacagaagta tatgaaggaa tgattatcgg
    33241 ggaaaacagc cgtgataatg atttgactgt caacatcacg aaagcaaaac aaatgacaaa
    33301 cgtacggtct gctactaaag accaaacatc tgtcatcaaa aaaccaaaac aattgacgtt
    33361 ggaagaatct ttggaattct tgaatgatga tgaatattgc gaagtaacac cagaaagcat
    33421 tcgcttgaga aaacaaattt tagagaaaaa tgctcgtgaa aaagcatcta aaaagaaaaa
    33481 ataatctgta aaaggatgaa gagagaaacc agcttgttat aaaaaatagc tggtttcttt
    33541 ttttgtccaa ctcttttctg tggagaagtc atctttgtca atggacataa attcagaaaa
    33601 tacgccgaaa gacgtttaaa agacttttaa aaaacttatt tctagagagt ttttgtttca
    33661 taataagata atgtttcacg aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa
    33721 atggcaagag aaaggagcgg gaagatgaac gacttattaa aacaatactt tggctatgat
    33781 gagtttagac ctggtcaaaa agaaattatc cagaaagtca ttgatcagga aaatgtatta
    33841 ggaatcatgc ctaccggaag tgggaaatca atctgttacc agcttccagc tttactatta
    33901 gatgggctta ctgtggttgt gtccccttta atctctttga tgaaggatca ggtggacgcg
    33961 gcgaatcaat taggtatacc cgccactttt atcaatagct cgttagatgg ttacgaaaca
    34021 gctagacgct tccaagaaat cgatagacag cagtaccgtt tactttatat cgctcctgaa
    34081 cgttttatca tgccggattt tatccaagca atgaagcgat ggaacgtccg tatgatcgct
    34141 attgacgagg cacattgtat ctctcagtgg gggcatgatt tccgcccaag ttatttacag
    34201 atggcgaacc agctagatca actgccaaat cgtcctgtaa ttgttgcttt gacagctaca
    34261 gcgactgtgc aagtagctgc tgacataaaa agacttttaa aaattccaga aaacaaccat
    34321 attcagactg gttttgaacg agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa
    34381 gaacaatatc taatcgaata tttaaagatc aacaaaaatc aatctggtat catttatgca
    34441 gcaacgagaa aagaagtaga ccgtctctat catttgctta aaaaattcga tttttccgtg
    34501 ggtagatatc atggtggtct aaatgagaat gagcgaacag aaatgcaaga agccttttta
    34561 tacgatcgct tgcagctgat tgtagcgaca aatgcatttg gtatgggtat aaataaaagc
    34621 aatgtccgtt ttgttatcca ttatcaaatt cctggctctc tagaagcata ctatcaggaa
    34681 gctggtcggg cgggaagaga tggtctttcc agtgaagcca tcctgctttt ctctcctcaa
    34741 gatatccaag tccaaaagtt ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa
    34801 aaagaatatg aaaaattaaa agcgatgaca gaatacgtgt atattgagtc ctgtcttcag
    34861 caatacatcc taaactattt tggcgagaca agcagtccgt gcaaccgttg tggtaactgt
    34921 ttagatgaca gagaaatagt agaagtaacg acagaagcac agatggttct ttcttgctta
    34981 aaacgtatgg gtgagaacta tggtaaacaa atgctgatga aagtcttagc aggatcgaaa
    35041 gaacaaaagc tgcaagcttt aggttttgga catttatcta cctatggact gatgaaaaac
    35101 caatcgcaaa aagaaacgat gcagttaatt gaatatttga tatcgaatgg ctatcttctg
    35161 accatcaatg gtgagtatcc tgttttgaaa gtaacagaaa gagggataca agtattgaaa
    35221 ggacaagaat ctgtttatcg aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa
    35281 accgatacac tatttgaagt gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc
    35341 gttcccccgt atgtcgtatt ttcagattcc acattgaaag aaatgagccg aatacgtcca
    35401 tcaagcagat tggagatgct tcagataaaa ggagttggcc aaagcaagct tgataaatat
    35461 ggcgaagcct ttttatctcg gataaaaaat gcggactcta acgttctgga taagtagaaa
    35521 gaacgaatac gtaaaaatga ataaaaacaa ctggttaaat ctgttttttt tgctccttat
    35581 gaaaatcaaa tatgttataa ttaaggaatt agaatgggag gtaagcacag atggattctt
    35641 tgtcgaaaaa acttgcgatc caagtattaa atgatgaagc agaacggatc aaacgattaa
    35701 tccgaaatca aaacaacagt ctctgtatct cacaatgcaa agccttcgaa gaagtggtag
    35761 atacacagat gtacggcttt tcccggcaag tcggttttgc tgtccgtata ggagtaatcg
    35821 gaaagaacga gggccagctg atgttaagtg agctagaacg agaattgaat cagttatata
    35881 gtgaggttta ccaagaaatt tatgataaaa aagaagttgg caaggaggaa tagacttgcc
    35941 taagaaagtg aaaaaaaggc atctattaga ctatagcatc ttgattccct atttaatttt
    36001 atgtgtattg ggattgatca tggtatatag ttctacgtcg tatctattat tagaaaacgg
    36061 acaaaatccg tccgcatcag tcattaacca gagtattttt tgggtgctta gcttgattgc
    36121 tatcgcacta ctttataaaa tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc
    36181 agccatcgca gttttgacga ttttgctgct gattgttgtg ttttttggta aggagatcaa
    36241 tggggcgaaa ggttggctgc aaattgcagg attctcgatc caacctgccg aatacctaaa
    36301 aatcatttct atctggtacc tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa
    36361 agattttctt gggactgtga aacgtccact tgcaatggtt atcggactta ctgcattagt
    36421 cgctattctt cccgattttg gtaatgcggc agttatcttt ttgattattt tggttctgct
    36481 tttagccagc ggagtcaatt atgtgtatac tttgattgta ggagtaggag gcttttgtct
    36541 aagtactttt acgatctggc tgatcaatat cacaaacgga aagatcttac ccggacgttt
    36601 acagtatatc tataatcgat ttgctattta ccaaaatcct ttctcagacg aattaaataa
    36661 agggcatcaa ctagtcaacg gctactatgc catgttcaac ggaggactat ttggcagggg
    36721 attaggtaac agtatccaga aaaaaggatt tttacaagaa gcacaaaccg attttattta
    36781 tgcaatcgta gtggaagaac tgggagtgat tatggggatt ttgatattag cgttgctatt
    36841 ctttatgatt gtgcgcattg tccttgtagg tatccgttca aaagatccgt tcaattcgct
    36901 tttgtgcatt gggatcggcg caatgttttt gatccaagta tttgtcaatc ttggaggaat
    36961 tactggcgtt attcctttga cagggatcac gttcccattt ttaagtcaag gaggctccag
    37021 tttgctgatg ctttcgattt gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg
    37081 caagagctta ggtttataat aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag
    37141 ttgctaacag gggtgaaatc gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta
    37201 aaactgttgg aatctatgca gaagaagatg aatattccgt acaccgtttc aaagcggatg
    37261 aagcatattt agtaggacaa ggaaaaaaac cgattgatgc gtatctagat attgaaggga
    37321 tcatttctat cgcgaaagaa tgcggagctg acgctattca tccgggttac ggtcttttgt
    37381 cggaaaactt gaattttgcg caacggtgcg aagaagaagg catcattttt gttggaccca
    37441 aactgcatca tttagatatt tttggagata aaatcaaagc aaaagctgca gctatcgaag
    37501 cggggattgc ttcgattcca ggcacagatg gaccaatcgc ttctatagac gatgccttgg
    37561 agtttgcgaa acaatacggt tatccgatta tgatcaaggc agctctaggc ggcggcggac
    37621 gcggcatgcg tgtagctcac gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa
    37681 gcgaagcaaa agcagccttt ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc
    37741 ctaagcatat cgaagtacag attctcgggg atacgcatgg aaacgtcatc catctttttg
    37801 aaagagattg ttctgttcag cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct
    37861 ctatgaatga acagcaacgt caaaaaatct gccaggcagc tgtccaattg atgaaatatg
    37921 tcgggtatgt caacgcaggt acagtagaat ttcttgttga aggagatgat ttttacttta
    37981 tcgaagtcaa ccctcgcgtc caagtggaac atacgatcac agaaatgatt acagatattg
    38041 atatcgtaac aacgcaactg ttgattgctc aaggtttgga tctgcataaa gaaatcggat
    38101 tgcctcagca agaaggaatt aaattaaatg gttcagccat tcaatgccga atcactacag
    38161 aagacccgtt gaataacttt ttaccagaca cagggaaaat cgatacgtat cgttcacctg
    38221 gtggttttgg cgtacgtctg gatgtcggaa atgcttacgc gggctatgta gtgacaccgt
    38281 attttgattc attgctagta aaagtatgta cgcatggtgc tacctttgaa acagccattc
    38341 agaaaatgga acgttgcttg aaagaattta gaatccgtgg agtgaaaaca aacattccat
    38401 ttatgctcaa tgtcatcaca catcctgaat tccaatcagg aaatgcaaaa acaaccttca
    38461 ttgatagtac agcaacatta tttgaatttc ctcgactacg agaccgcgga aacaaaacga
    38521 tgaaatatat cggtgaaatt actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac
    38581 cattttatga agaaccacgt atgcctaaag atctaatcac aagaactgat tacgtaacgg
    38641 ctaagaatgt attagatgcg aagggtgccg atgctttggt cgaatggatc aaaggacagg
    38701 aaaatttgtt gttgacggat acgacattta gagacgccca tcaaagtctg ctagcaactc
    38761 gtgttcggac aaaggatttc aagcagattg cacgtttgac tggagaaggt cttcctgaac
    38821 tcttttcaag tgagatgtgg gggggagcaa catttgatgt ggcttaccgc tttttgaatg
    38881 aagatccttg gcaacggttg agaaaaattc gttctttaat gcctaatacg ttgctgcaaa
    38941 tgcttttcag aggttcaaat gccgttgggt attctaatta tcctgacaat gtattggtgg
    39001 aatttgtcaa agaagcagca gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga
    39061 actggactcc acaaatggaa aaaagtatcc aagctgtacg ggatactggt aaaatcgcag
    39121 aggcagctat ttgctatact ggcgatatca acgatccaag tcgggcaaaa tacaatgttc
    39181 agtattataa agacatggca aaggaactag aacaactggg tgcgcatatc attgcaatca
    39241 aagatatggc tgggctgctt aaaccgcagg cagcttatcg tttgattagt gaattgaaag
    39301 aaacgacaga tctgccaatc catctgcata ctcatgatac cagcgggaat ggaatcatca
    39361 cttattctgc tgcttcaaaa gcaggagtag acatcgttga cgtggcaatg agtgcaatga
    39421 gtgggaatac tagtcagcct agcatgagca gcttgtatta tgcactggtc aatggtccac
    39481 ggctgccaga aatcacgatt gaaaatgcac agaaattaaa ccattattgg gaagatgtgc
    39541 gcatgtatta caaaccgttt gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc
    39601 atgaaatgcc aggaggacag tattcgaatc tacagcaaca ggcaaaagct gtcggattag
    39661 gtcatcgctg ggatgaaatc aaacaaatgt atcatactgt caacttgatg tttggcgata
    39721 tcgttaaagt aactccatca tctaaagtag ttggagatat ggcactattt atggtccaaa
    39781 atgatcttac agaagaagat atttatgaaa aaggcgaaac actaagtttc cctgaatctg
    39841 ttgtgacttt cttccaagga gaactagggc aacccgttgg cggatttcct aaaaaattgc
    39901 aaaagattat tcttaaaggc agacctgcat tgaatgaacg tccaggtctt ttggctgaat
    39961 cagtcgactt caacgaagta aaaaaagaat tggcagaaaa aattggctat gaaccaaaac
    40021 aagacgaagt attaagctat ttgatgtatc cgcaagtctt tttggactat caaaaagcat
    40081 ataatcaatt tggcgatgtt acattactgg acacaccaac atttttccaa ggtatacgtt
    40141 tgggtgaaac aatcaatgta caaatcgaac gcggaaaaac gcttatcatt cgtttagatg
    40201 aaatcggaga acccgatatc gaagggaatc gcgtactgtt cttcaatttg aatggacaaa
    40261 gacgtgaaat cactatcaaa gataattcga tcatcagtac ggtccaaacc cgattgaaag
    40321 ctgaaccgac aaaccgagaa caaattggag caacgatgtc tggttctgtt ttggatgtat
    40381 tagtgaaaaa aggcgataac gttaaaaaag gtgatacgtt gatgattact gaagcgatga
    40441 aaatggaaac ggccatcgaa gcacgttttg acggagaagt cgcacatgta tatgtgtctt
    40501 caggagacac tatttcgtct ggtgaccttt tgattgaagt gactgagaaa taataagcga
    40561 aaggtgggga aacatgaaaa gactgggttt gttttggctg attctgctat tagttattgt
    40621 ggcattctac atggaaccag tcttgtttcc gccaaaactt gaagtgaagg aaaactctga
    40681 tcaacaaata tcaaaccaaa tgacaacgtg gaaataccag gagcttaaag caactggttt
    40741 ttctacttat atcggaaaac cgatcgatca gctggaaaag gattttggcc aagcctatga
    40801 tcgattaact agcggatttg gttttgaaac aagatattat gaaaataaag ctagtcatct
    40861 cacatttgaa gcgaacatcc aagacagcag agtcacaact gttaaagtac tgcaaacagg
    40921 gcctgctgat atcgcgccgt tttatttagg gatgacgatg caagatctta cgaaaatcac
    40981 tactatctat actaatttca cttttgagta ccatgataca gaagttggaa tcgaactaat
    41041 ggaagaggat atgaattatc gtccgctaat tgcttttgat aacgacactt ttgctatttt
    41101 attttttgat caaacaacaa acggcctatt ttcagttgtg tatttagata aagatagtct
    41161 tttgaaacta ttgccatatc aagtgtttgg tgaaggcctt ccacattatc aagcagataa
    41221 atcggctgat tgggaatcaa tcaatcgcgt taaagaacga aaaagtacga ctttattaaa
    41281 tatgcttaga agagaagacg aacttcccat atacaatcaa acacttgcct ttcaaaacca
    41341 cagtcagctg ctactacatg attttttgac aaggcctgag gaaatattgt cagaggaacg
    41401 tatggctgat tggcaaaaat cgctaggcag tgctcaaacg acagtgaaat tctcactgac
    41461 gaatgatgaa ttggagaagt taatcagtaa acaaaaatta gaaaatacta gtgggctatt
    41521 tattcatccg gcgattgacg ctactttttc gtttttatat tggtacagcg atccttacaa
    41581 ccatgaccgt ttcatggacc cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa
    41641 tatgttagtc ttattgcaag aagaaaacgg agaaagtagt gatagcagtg atcgttaacg
    41701 aagagctgtt tcaactggag gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta
    41761 aaacgatggc ttcttatctt gaacataaac agtcaatgga agaatctgca aaagtttgcc
    41821 agttgaaaga agcgttcttg tctaagaaag aagcatttga aaatgtagcg gcatatggtg
    41881 agtatgcacc tgattatcgt gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg
    41941 atatggatga gcatgttgca gctttccgat atagtgaaac acagctgcaa agtctgttgg
    42001 atcaaattgt ccaagtactt gccgaagaag tttctaatga aatcaaagta gatgcaggta
    42061 gcccgttttt tgaaacgaaa aaacactttg gatgcggagg taattgtcat gcaattagac
    42121 aatagtgaat ttgaaataac aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa
    42181 caattaaaga atctgaaaaa gttcggcttg ttgcattatg tctcaagaag aatgaaatat
    42241 gtcgtgcttt acttaaatga agagacattg gaagaaacag aagaacgaat ccgtaaatta
    42301 cattttgttc gaaacgtaga acgttcttac cgaccggata tcgaaatgaa ttttgccgaa
    42361 aaaataggaa acaaaacagt attaaaagac gaaggtttcg aaattgaaga gctaaataca
    42421 gaaatccgat tagctgaaca tctttaaaaa tctcttatga gcagttttta aataaaaaat
    42481 aacttggata acgatcgatt tttgatcatt gtccaagtat ttttttggta tactagtacc
    42541 agtaccattt ctaaggagtg agtagatgcg gataatatca ggaaattatg gcgggcgccg
    42601 tttaaaaagt ttagcagggg cgaacaccag accaactaca gataaagtaa aagaatccat
    42661 attcaatatg attggaccat attttgatgg agagacagta ttggatctgt ttgcaggaag
    42721 cggtggttta gctatcgaag cagtatctag aggctgcagt catgcggtat gcgtagacaa
    42781 aaattaccaa gcgctaaaaa tcatcaaaga aaacatcgaa attacgaaag aaccagagaa
    42841 atttaccaca ataaaacttg atgcggacaa agcaatcgaa cggctagcag cggagaaaca
    42901 acaattcgac tacttgtttc ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat
    42961 ccataaaatg gaagaactgg gacttttttc taaaaccgca gttatcgtat gcgaaacgga
    43021 taaaacagtc gaattgccag aaactgtggc tggattccat caaataagaa aacagattta
    43081 tggcataacg gccgtaacta tctatagaaa agagggaaat caatgaaacg tgcattattc
    43141 ccaggaagtt ttgatccttt tacaaaaggt cacttagata cggtggaacg tgcggctaaa
    43201 ttattcgatg aggtagtaat tggtgttttt atcaatactt caaagaaaag tttgtttccc
    43261 ccagaagaaa gaatgacatt gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc
    43321 atgcatcaag aaaatcaatt gacggtagaa acagcaaaag agattggtgc agatgcgttg
    43381 atcagaggca tacgaagtat caaagacttt gaatatgaac gagagattgc tcagatgaat
    43441 catcatctat atgatgaatt ggaaactgtc ttcttattag caaaaccaga atatagtcac
    43501 gtgagttcca gtattttaaa agaagtactg cactttggcg gagatgtgag cagctactta
    43561 ccgccagtga tcaacgaagc tttagctgga aagcgtgaat caaatgaaag ctaaacaatg
    43621 gttgaaaaca gtactgatta tcatatttgg aatgttgatc attggatttg tagtagttcc
    43681 cttgccgtat tatgtagaat caccgggagc cacgatcgat ctgaaagaac taattacggt
    43741 caatgataag gaagataaag gttctgggtc tttctctctt acctctgttg gtatcaggca
    43801 agctacaggt ttcttagctt taaaagctaa aatgacccct tttgaagaat tgatcagtga
    43861 agaagaattg actggtgggg cgacaaatga agaatataat caaatgcaac agtattatat
    43921 ggattcttcc caaaatgcag caatcgaaca agcgttaaaa cttgcagaca agccttataa
    43981 aatggaattc aaaggcgtat atgtcatgag tgtagagaaa acatcgaatt tctttggcaa
    44041 gctttcagtt ggagatacag taacaaaagt agacgggaaa agctttcaaa gtacagaagc
    44101 atttatggat tatgtaaaaa gccaaaaagt tggacaaaca atcgaaatca gttatttgca
    44161 taatggtgaa gaaaaaacag cttctggtga tctgatcgag ttaccaacag ataaaaaggc
    44221 aggaataggt atcacgctta ctgaccatac cgagatcgaa tcagatacag atgttcgtat
    44281 taattctggc tctatcggtg ggccatcagc gggattgatg ttcactttag aaatctatga
    44341 acaagtcact ggtaaaaact tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa
    44401 cagtgaagga gaagttggcc gaatcggagg aatcgataaa aaagtggcaa gtgcggataa
    44461 agctggcgta gagattttct ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga
    44521 atatcctgaa ataaaaacca actacgaaga agcaaaagct gccgctaaaa aattgaattc
    44581 ctcaatgaaa atcgtaccag taaaaacagt acaagaagcg cttgactatc tagagaaaat
    44641 gaaatgaaac taaataatta aagaggggtt gtgacacgag ttctgtcaca acccctcttt
    44701 ttcgtcaaaa atcttcacga taaaaataaa aacaaaagaa aaaattgacg taattagatg
    44761 ttgaagggac gattttgcca gagctcactg tttcttcaat tttaaatgaa aggagtaatg
    44821 aatgattttt actggtcatt ctttgaaaaa agaacaatga tagaaaaaat cagcaagaaa
    44881 atactagctt cacccaaatt gatcatttgg atgtctatga tagttcttat cttttgtatc
    44941 gcaagtggtt ttttattatc aaatttgcta ttttatagag aaacagaaga gactgctgca
    45001 aaagaaatac tttcttcgtt tagttccagt ggagaagatc catcagctga aggaaacgat
    45061 gtagaagagg atgctcgtcc aaaggtcatg tacaccgata tcaaaggaag tgtgaaggag
    45121 cctggtatct attccttttc ttcagaagaa cgggtctatg atgtactaaa gcgtgcaggt
    45181 ggattattag aagaagcgga tagcgatcga atcaactttt ctgcgaagat agaagatcag
    45241 caagtcctat atatccctgc tgtaggtgaa gagctgccag aacatcttga tcagttggca
    45301 tcctcggaag gaaaacagcc tacagcagat tccgagcctc ccaaaataaa tatcaataca
    45361 gccactccat cagaacttca gcagattcct gggatcggat cagtcaaagc gcaagaaatc
    45421 attcgattta gagaagaaaa cggatccttc cagaaagtgg aagacctgca ggagatttct
    45481 ggtatcggtg aaaagacagt tgaaaaacta aaaaacttcg ttacaattaa atagaatgat
    45541 tcaaaatcga ctggcacgag caataaatgg acgctaatgg atactaagaa taatccaatt
    45601 ctaagagctt ggcaggacga ggaggtagta tttttggata ggaaatagct tgattgttcg
    45661 caaagaaaca ttaaaaaaac caatgctgaa tgtctatctc caaaataaaa tatctgggat
    45721 tcatattatg aatacggctg tcagcggcaa taacagtcaa gctctacgag aacgctttgc
    45781 caaagatgta cttagctata cagcagataa agtatttatt ctcattggga cgaatgatct
    45841 agcagagcat aaacaacttt ctaaagaaac gtaccaaaaa atctgcagtg gatgatagtt
    45901 cagctgaaaa aagactatag ttctgatcat gctttcttta tcacgccacc agcagtcgat
    45961 gagacaaaac aaaaatatcg gaataacaag ttgatcaaag aatatatctc tgttctcaca
    46021 gaagtagcaa aacgccatga ttgtgaagta tcgattttta tacggaattt cttgaacaaa
    46081 tggattcgaa gaatttttat aatgggctaa tggatgacgg acttcatttc ggcagtgaag
    46141 ggtaggatct tttgtcagac aggatgattc gttatattta aagaatattt tgtttaagca
    46201 atgagtactt tttaatcaac ttgaaaaaac ggaggaatat aaaacatgtt agaaaaatct
    46261 tcaaaagcat gcgatacgaa taaagaaaaa gttactgata agtcacataa tgaacgcatt
    46321 ccttgggacc agtactttat ggctcaggca gtactgttat ctttacgtag tacgtgtaca
    46381 cgtttagaag taggagcgac actcgttcga gaaaaaagga tcattgctgg cggctataat
    46441 ggagcagtca gtggagatgt ccactgcata gatgaagggt gttacgtcgt ggatggacac
    46501 tgcttgcgaa cgatccatgc agagatgaat gcactcctac agtgtgcaaa acttggtatc
    46561 ccgacggaag gttcagaaat ctatgtcaca cattttccat gtttggcctg tacaaaggct
    46621 ttgttgcaag ctggtgtgaa agggatcaat tatctccatg actatcgaaa tgatccttat
    46681 gcccaagcgt tgatcgaaca attaggtgta tctgtacacc aagttgcttt agactcacag
    46741 catttttcaa aacttcaaaa tgaattaaca ggaaaacatg tctagcgttt tagaaaggca
    46801 tgttcgaaat cggttgattt ttccggcaat tacgtcgatg attatctgtt tgttgtatta
    46861 cactgacaat tggggttatc gtttgcttct tgccggaatt tttttatggt tttcgctaaa
    46921 agaaggacgt tttatcgcgt acttgtgtat tttgtgtagc ataacggtat ccatttctta
    46981 taggctacaa gagtattcat ttttagagga aaaaacaaca tctgcgcaaa ttactgtatt
    47041 tacagatacg attcgtgcaa atggtaattt tttgacaatg gaaggaaggt tatcagagaa
    47101 taaacaaaag ctccagcttt cttatcaagt aaacacagaa gcagaattga aaacatggct
    47161 taacttccga ggaaagggaa tagaattgac tgctgaaggc gagttcatct ctccagcaaa
    47221 aaagcggaat ctttataatt ttgaccaaga agcttacttc aggaaccata gaatttctgg
    47281 acggttccaa attcaaaaga ttgaacagat caccatgcaa aatcactggc gaaactggct
    47341 tcagagaaaa agaggatctt ttatctcctt tgtccaatct gaatttccgc aaaaaacaag
    47401 tgtatatatt aattctttat tatggggcta taaagatgca ggattcagag aaccagaaga
    47461 aatttttagg gagagtggtt tgctgcatct gttcagtcta tcgggtctac acatccaatt
    47521 ttatttaggg tggctgtatt atttgtttcg acgtatcgga tggccactac acgtcagtat
    47581 tattccgttg agtctactaa ctatctgtta tgtttctatt gcaggaagtt ctatttctgt
    47641 tttgcgtgct agtttgctgt ttttattgcg attgctagtc aaattgctga ataagcatta
    47701 ttccaccatg gacttatttg cagttgtctt ttggcttctt ttactatttg atcctcgatt
    47761 tctttttcat gcgggtggac agcttagttt tttcatgagt tttttgttta tcctgatttc
    47821 ttttgatgga gattggttga aaaaaacggc ctctcacgtc ctgtttacta tgatcacgat
    47881 gccgttgatt gtatggcatt tttatgagtg gcctatttta ggtagtcttt ttacattact
    47941 gattgcaccg gtattcaagt ttctttttct tcctggtgtg ttatttctca cactcttcaa
    48001 tcgctggctg ccggaaactt tacttttgct gatagaagag ggattaatct tatttgagcg
    48061 aataatcgat ttttcttcag ggtcgtctct cattatagga cgtttgcccc ttgtgctatc
    48121 tgggatgtat attttcggat tactgatttg tatggatagg ttgaaagaac aacctgtgaa
    48181 acacttgttt tatgttggtc ttttttcttt tatttttttg actttgccat ttttacgctt
    48241 ttcttcaacg atcgctttta tcgatgtcgg tcaaggagac agtatctttt tgcaaagtcc
    48301 ttttcgaaaa gaaacgattt tgatcgatac tggaggtcag ttacactttg aaagagaaaa
    48361 gtgggcgcaa ggtatgatac gtcctcctgc tgaaagaaat atcgttccat atctaaaagg
    48421 tcaggggata agtgtaatcg ataaactcat cttgacacac gacgatacgg atcatgtggg
    48481 agaattaccc acgttatctc agcattttac tatcaaaaaa atttatatcg gttggggcgc
    48541 tggacgaaat gagccattac aaaggcattt atctgaagct caaaaaaatg gtactgagat
    48601 cattgaaatc aaacatgggg acaggatcag cggttattat gatctttatg tattgactcc
    48661 ttttgaaaag ggagagggaa gaaatgaaga ttcgattggt ctatggatgg aatataacaa
    48721 taggcgcttt ttatttttag gagatttgaa tcaagcaatg gaaaaacaat tgcttttgat
    48781 ttatcctaat ctgaaagccg atgtagtcaa attaggtcat cacggtagcc gtacgtcaag
    48841 taacacagat tttctttccc atattgaagc aaaacatggg attatatcct gcggcgtaaa
    48901 taatcgttat ggtcacccgc acagcgaagt attggagaca ctagaagaaa atcagataaa
    48961 aacatggcga acggatcaac aaggaatgat cacctatcgc tggcatccag tttttcatcc
    49021 tcatgggcta gtggaaacaa tgatagacta gtaattctca tctgctcatg gtaggataat
    49081 ttttgaaagt ggtgagtaat ttgagtatac aagcagagtt acaaaaaata cgtaaagaaa
    49141 aacttgcatc ttgttacttg attttaggaa cagaaaagtt tctccaagat caagttcgga
    49201 cagaaattgt caaaaaaatc caattagagg gcgaagatga cttaaatttt ttaagttttg
    49261 acatggaaaa tgcaagttta gatgaggtag tagctgaagc agagacgttg ccgtttttcg
    49321 gagatcaacg gttagtattc gtagagaatc cttatttttt aacaggtgaa aaagtaaaca
    49381 atggaatcga acaaaatact gatttattga cggcttattt gaaagaacca ttagaatcga
    49441 ctgtattagt attctttgcg ccatatgaaa agttggatga acggaaaaaa gtaacgaaac
    49501 aattgaaaaa aacagcaatt atcatcaatg tccaacagtt gaatgaaaaa gaagtacgtc
    49561 aatatctgat gaatacctta gagaattcct caatcaaaat ggaacgaagt gcaattgatt
    49621 tattcttgag gttgaccgac ttagatttat ccaaaatgat gagagagtta cagaaattgg
    49681 tcctttatgg acaaaatcaa cagcaaatca cggtaagaga agtagaagaa ttagttccta
    49741 aaacgttaga acataatatt tttgacatga cccaatatat tttaaaaggg aagacggaac
    49801 aagctttgcg tttatacgaa gatttagtta tgcaagggga agaaacaatc aaaattaatg
    49861 cgattttgct ttctcaatta cgtttatttt tacagacaaa atttcttgta aagattggtt
    49921 atcagcaggc aaatattgct gaaacattga aaatccatcc atatcgtgta aaacttgcca
    49981 tgcaggaagt aaaaaaattc gatgaacatt tgctggtaca gttgtttgat caattagttg
    50041 agatggatta tcaaatcaaa acgggacaaa tcgaaaaaga attaagtttt caactattcg
    50101 ttttgcgcac agggcagttg ttaagaaatc gataagaaga aagacgaggg caaagcatgc
    50161 gggaaggtcg tttgtttttg tccttttttt gacaaataaa aaaatcggcc gaagccgatt
    50221 cttttattta gcgatttttt tgcttaaacg agatttgtcg cggttagctt tgtttttgtg
    50281 gattagacct tttgattcag ccatatcaat agctttaaca gcttctttat ataacgcatc
    50341 tacgttgtca gcacctgaag ctacagcatc ttcaaatttt ttgattgcag tacgcatagc
    50401 gcttgtttga gatgaatttt tcacattagc gttttcgctt gtacgaacac gtttgattgc
    50461 agattcaatg tttggcatta gtttcacctc cgataactta agttcttacg cgtgttaccg
    50521 cataattcaa cattcattat tatacctaaa ggcagcgtgc tttgcaatat gaacatgaca
    50581 agtttttttc cttaacagtc ttttattagg attttttttc agtaaaaacg gatagtacaa
    50641 gctgaaaaaa atcaaaaaga cgctattgtg ggaggcaata gcgtcaaacc aaactagatt
    50701 tttatttatc tatttaaaca aagtttgaat gggaatgttt ggtactaagt tattatataa
    50761 caaagagaac cgtttgcaaa gttttttttg aaaagggata cgattccctg ttgttctttg
    50821 tttttctatt aagaaactgc agttatttta taaccagtat cctttgatca gtaaaggcta
    50881 ttctggtgta ccgattattt taatgtattc atcgatattt tttatcaaca ttttttttat
    50941 gaccaactgg ttccgacggt tccatttgcc acttacttcc agcacatatt tatttagggg
    51001 caaacgtaag atatattttg ccaaatagcg atctttaagc aggccattga tcacacggtc
    51061 ctcaagctgc aagttgatcc cagtcagcat cattggctgt tcttgtaaaa cgtacgtatc
    51121 aattaaaaga ccttcaaaag tttccacaag cgtcttcctt tcttttcaaa gtaacctgaa
    51181 aacaggcact aattcttcca ttttgatatt cgttgaagtg gatgcgatca ttctttcttt
    51241 tgtccactgt aatctagtat aacaaaaaag agcagaagag ccaaaaagaa aaaagagcat
    51301 cgaattctat aggaagtctc attggcacgg cctaaattat tacaaaacat tgattaaaaa
    51361 aagcccctgc atcgcaggag catcccctat atcgttttaa taggtcaatc aaaaagcagt
    51421 atcaaacaca ttttgtatca aatcaggaaa taatagataa gctaagccca gaatcaaaag
    51481 taccgctaga agaaaccagc cgattgttcc tatgaaatac tttaattttt cgaacattgt
    51541 atcacctcgt tgtcagtata tcacggatca gtaaattggg ggtgttaaga atctcctaaa
    51601 atagacaaaa tttgttctat tttcctaaag caaatacttg taagatacag taagtgatac
    51661 cagaagcaat gaccggtccg gcggcgattc ctcttaagaa gacaacacct aaaatcgtgc
    51721 cgaaaacaag tgcaacagtg atttgtggat cagaagcaat caatcctaca cccttggaag
    51781 ataagacagc cactagccca ccgcaaaaaa cagctaccca gccaataggg gatttaaagg
    51841 cttctaatag atcacgtaga ccgatttctc ctgttgcgat agggattaga atagccgctg
    51901 tgatgatcgt caccccaata ttgattcctt tgccttgaag ccaagggaaa aaggaacggg
    51961 aggctggtat gagcttcata atcaaaacaa aagctgccgc tatcatcaga ctttgatttt
    52021 tagctaaaat tgctacaagt aaaataattc ctaaaaaaag ccaactttcc atgaaatata
    52081 ttcctcactt tcttcgaaac aatcataaca tgtttttaga aagttacaaa agtaggaatt
    52141 catccttcaa gacctattca taagctgttt ttttcataaa ataaacaaaa tcataaaaaa
    52201 acactttgct tatttgacag aagacagccg cgctttgtat agtggacaag acttacattt
    52261 ttttgaagaa tcagaaagat caggtaaagg tcttagtaaa cgcaagccat tcagaataac
    52321 caatattgtg cttccttcgt gtcctacaac tccaagaggt agatttaaaa attgcaggaa
    52381 gttagaagca atcaacaaga gaataacaag aatcgaaaaa ataatatttt gtttgatgat
    52441 ccgccgcatt tttaaagata gccgatgact gtagacaaat ttatttaagt cgcttttcat
    52501 taagacgatg tctgcgatat ccatagcggc atctgtccct tgccccatag cgacacctaa
    52561 agaagcggct gctaaagcag gcgcatcatt gactccatca ccaaccatcg catttacttg
    52621 gtaaagttca ttctcttttt tgactagttg tgttttttct tccggagtac agcttgcata
    52681 gtaatgttca atccctactt gttcagcaat ggcctttgct gtgccagaat ggtcgccggt
    52741 tatcatactt gtatggatgt tttgggattt aaagtaggaa attacttgtt gggtatttgc
    52801 ttttggaata tctagcagac ctaaaacagc aattaactgc tgatctttag aaagataaat
    52861 cacggttttg ccttgatttt ccaaacgttc gatcatttcc gcaatctctt tgctgattat
    52921 catcgtctca ggatcatatg cgtgcttgcc aactttccat tgcgcaccta aataggtagt
    52981 ttgtaatcca aaaccagtaa tttcttctat tggtaagtat tggatttctt caggtattgt
    53041 gcaatcgaaa tattgcaaaa tcgcatgagc aagtggatgc gtcgtttttt gttccatagc
    53101 aacaagaagt tgttgtgcca aatccttttc atcaaagaaa aagctgtcag ttacaactgg
    53161 ttttccttta gtgagcgttc ctgttttatc aaaggcaatc gcttttaatt cagaaagctt
    53221 ttctaaatgg ataccgcctt tgattaaaat cccatttttt gcagaatttg aaagagcagc
    53281 tagagaagct ggtgtagcag atgcgacaag cgcacaagga gaagccacaa ctaataatac
    53341 cattcctcga taaaagcttt catcccaact ccaacctagg aaaaagtggg ggagaaggat
    53401 cattagagga acagcaagca aaatcaattt tacataatta ttttcgattc gttcgataaa
    53461 tgaagctgtt tgagttggcg tattctgcgc ttcttctact aattggataa tcttagcaaa
    53521 gacagtttcg gaactcgttt ttgttactcg aatcgtgatg ggatttcctt gattcagcgt
    53581 tcctgcatac atctcatcgc ctaaatattt ttctacaggc acagattcac cgcttaatgc
    53641 agactcatcg atcgttgcct gtgtgctttc taaaaagcca tctatcggaa cagctgctcc
    53701 tttagcgaca aataccaagt tatccacctt tagttgagaa acatctactt cttgcatttg
    53761 tccattttct tttaataatt gggctttttg tggttgaaga ttcatcaaag cagtaatttc
    53821 tttttgactt ttattagttg tgtattcctc taatgcaccg cttaaacaaa agatgaatgt
    53881 caacattgct ccttcaaagt aattaccgat caaacaggca ccaatagcag ctaaagccat
    53941 caataaatcc acattcagtg tccgatcatt ccacaattct ttcaagcctt ccgatgtttg
    54001 tttatatcca ccgatgataa tggcactgat ataaaaaagt ggggtgatgg taagagagaa
    54061 tcggtcgaag ataaaaccga agatcataaa taaaaggcag ccgattgttg ctgcaaaagc
    54121 ttttttctga ttagtcatcg ttattagacc tccttgtcaa aacaaggata gcataataaa
    54181 aatgtaattg ataataataa cagctaaatg agaacgataa tggttatcat ttggtgatta
    54241 agcttgatgt tgtttttatt atttataatg attattatta aagaaaataa aaaaccagaa
    54301 catttctcaa ataagagaag tgttctggtt ttttctacat tgcaataaac caataaccaa
    54361 tcaaaatgat accaaggata atacgatacc agccaaaagc ggtaaagtcg tttttcttca
    54421 aatagcttag taagaatttg atagctaaga cggaaaccac aaaagatacg aatgttccta
    54481 caagaagaac aacagtttct tccattttga atgaattccc ttcagctaaa tatttgacga
    54541 tcttcaagcc gcttgcgcca aacatgacag gaatccctaa gaaaaatgaa aactctgtag
    54601 cgacaaaacg cgaagcacca atcaagatag atcctaggat cgttgcgcca gaacgggatg
    54661 ttccgggaac aagtgccaag acttggaata acccaacaag aagagctgct tggtacgtaa
    54721 agtcattcag agaagtccaa cgaggtgttt tgtttttgtt gcgtttttcg acaataataa
    54781 aggcaatacc gtaaacgatc aaaacaatgg caactggtaa gaacttgtgg aaatttgctt
    54841 ctaaccaatc atccaaaggc aaaccaatga tgatggctgg tatgcaagca acgatcactt
    54901 taaaccaaag tgtccacgta tctttttttt ctaccgttga tttacttgga gaaaatgggt
    54961 ttaacttgtg aaaataaaga acgacgacag ccaagatcgc ccctaattgg ataacgacat
    55021 tgaacatcga cataaattgt gaactggcat ccagtttgat gaattcatct gctaggatca
    55081 aatgaccagt actactgatg ggcagccatt ccgttattcc ttcaataata ccgagaatca
    55141 ctgctttgat aatatttgca aaaaacataa attccttcct tcccaattaa gatacatctt
    55201 atagtatacc gttatttttt gagaaaccca atctgttttt taatattaag aaaaacttga
    55261 gaaataaagc agataagtac acgaagagaa aaaagaagac aaagccctcg ttcagacttt
    55321 gccttttatg attctctggt aaaaattaaa atatccccat tatagaaacg agtcgcgact
    55381 atgtgttttc ccgttatcga tataagacgt atcattgatg ccgtggatag aaaatcgacc
    55441 gtttgcataa gtgacctttg ttacgctgcc atttaaaagc ccagcacgaa caggctgttt
    55501 aggatcgatc agattaagca aaaaggcgat cgtcaaacca tgagatacga ctaaaacatt
    55561 tccaccacca tttttttcgc agtgatgggc gatatcttca aatcctgtcc atacacggtc
    55621 tcgcaatgtt tcataagttt ccgcccaccc agcggtatct aagttgtata cagcattagc
    55681 aatttgttca aaagaaactt ctgtggtaaa catttcatca tatgttttaa aattcaatac
    55741 acgagggatc acaccccaca tttccatgtc atatccacct tcaagagacc cgaaacacca
    55801 ttctctaatc cttttttcaa taaaataagg aatttctttt cccttagaat gttctcctag
    55861 aataatacgg gcagtttcga ttgcacgacc actgtcacta gaataagcaa atgaaaagtc
    55921 gacatttttt aacccgcgac ctaaataatg aatgccttct tgtccttgct ttgtgagggg
    55981 tgtatcacac cagccttgta ctcgttcaat tgtattgaac atcgtttttc cgtgtcgtac
    56041 gatataaagt gtagtttctg tcattttatt ctcctcctaa gtcgttttaa cgattgaaaa
    56101 aaggatttgt ccttttttcc tgttcaatcg tcgttgcatc gccatgtcct ggatagactg
    56161 caaattcatc aggtaataca aacagctggg tccgaatact atgaagtaat tgttctaaat
    56221 tccctgtata cagatcggtt ctaccgatac ttcctctaaa cagggcgtcg ccagaaacga
    56281 caaaatcatc aaaaataaag ctgacgcttc ctatagaatg ccctggggta gggacgacgc
    56341 gaaaagacat gtttcctaaa gtataagttt tcatttcaaa ttcatattct gcaggcgaga
    56401 caataacatc agcaatatca tcatgtctac cgagtccaga aagattcaaa atcggatcac
    56461 ctagccattt ttgctctagt gggctgacat aaactgggat ttgataatac gcgcgtaaag
    56521 gttccactgc accgatatga tcgtagtgag tatgggtaag caaaatcgct accggctgtt
    56581 ggtttgtttt tttgatttct tgtttgattc gttccgcatc ttctccaggg tcaatgatca
    56641 aaagtgcttc ttcgttgtag accaaataac agttttcttc aatcattcct gtttttaatc
    56701 tttcgatatg catgtgcatt cctccttgat agccaaaact tctttcatcc attagtataa
    56761 cgcattcatt gctaggaaac aaaaacaggt acttatttct ctttacttat ctgaattatc
    56821 agactatatg aaaagtattt ggatattcta aagagaagga aagtaagttt tcatagcatt
    56881 ttaatatgtc cttgttacat tagattcatc caaaaatcca aattctatat cctatatccc
    56941 tgcctaatca gcagggtgtt tttttatgtt tggaaagaga gctctttttg ctaaactagg
    57001 ttttttcagg taatcttaga gagaaagagg tggtggaaat gatcaaacag tcaaaaatat
    57061 ctgtgcggca cttagtagaa tttattttgc gacgtggaag tatcgacaat cgtaaaaaaa
    57121 gtaaccatac tgcattagaa ggagcgagaa tccaccgcaa gctccaaaaa gatgcgggag
    57181 aaaactatga aaaagaagtc tttttaaaaa caacagttca attagatgaa tattacctga
    57241 tcgttgaagg ccgtgcagac ggtattttca aaagagacgg tatttattat atagatgaaa
    57301 tcaaaacctc cgaaccacga tttgaagatt tagaaccaga acaagtggaa cttttctttc
    57361 atcaagcaag ggtgtatgcc tatatctata gtcaagaaca tgatttacaa gaaataaatc
    57421 tgcaattgac ttacttccag acggcagaag aagtgatcac gagaaaggtt gaacatcaga
    57481 caagagagca gttagaaact ttttttaaaa agctgactga agattataaa gaatggcttg
    57541 tttttcaaga aaactggcgc actgtacgca atgcctcttt gatggctttg aaattccccc
    57601 atgatgagta taggaaaggt cagcgtgaac tagcggtagc tgcttataaa acgatccgta
    57661 cgaaacagaa attgtttgta gaagcaccta ccggaacagg aaaaacaatc tctacgttat
    57721 tccccgcttt gaaagctgta ggggaagaag agggagaaaa gatcttttat ctaacggcta
    57781 aaacaataac tagacaagta gctgaagatg ctatgacagc attaaaagat acaggagctg
    57841 aagtaaaaag tgtcactttg acggcgaaag acaagatctg ctttttagat gagacgacct
    57901 gtaatccaga tcagtgccca tatgccaatg gttattacaa tcggattaat gaaggtttat
    57961 gggatctttt gaatcatgag aatcagatca ctcgtgaagt catcgaaact tatgcaagaa
    58021 agcatacact ttgtcctttc gaactatcat tagatgtcag tatctggtgt gacgtaatca
    58081 ttggggatta caattattta tttgatccaa ctgtttattt aagacgtttt tttgaagatg
    58141 aaaaaaacga ggactatctg tttttgattg acgaagcaca taatttggtg aatcgttccc
    58201 gagagatgta ttcggcagaa ctatcttatg aaaaaacgaa acgaagtaag gaagctatac
    58261 caaaggaatt caaaaaactt catcgccgtt tcaacaaact attaaaagaa tttgattcga
    58321 tccgagaaat agcaaaagaa gatcattggg attatcatca tcaaaaagca ccggctgaat
    58381 ctttagtaaa ggcaggttat caattaagcg aaaaaattaa agaatggctg gcagaatttc
    58441 ctgagcaccc atcacaagaa caattgcttc cttattattt tgatttgctt cattttttga
    58501 aggtcagtga attttatgat gatcactacg aaacaacagt cgaaaaaacg tatcgcgatc
    58561 tgatagtaaa agagttttgt attgatccaa gcttgtttct tgagcagtcg ttggataaag
    58621 gaaaaagcag tattcttttt tctgctagtt tttcaccact ctcttattat caggaaacac
    58681 tggggggaca aaaaagtctg gcttacaaac tgccgagtcc tttcccagaa ggaaaccagc
    58741 aagttttcat cgcaaattac ctagagacga cttatcgaaa aagggaacac agtctggcaa
    58801 aattagtaga gacgattcat gcttttgctg atggaaaggt cgggaattat ttcgttttct
    58861 ttccttctta tcaatattta gatcttgcgg tggaagcatt cagacagcaa tatcccgaca
    58921 gcaatgtgct aatccaagaa acggacatga atgaggaaga acgcgaacga tttttaacaa
    58981 aatttcaaac aaatcccgct gaaacattga ttggattttg cgtacttggc gggatttttt
    59041 cagaaggaat cgatttgaga ggggaccgat tgatcggcag tatgatcgta ggtgtaggtt
    59101 tgccgcagat gaatcatgag caggaactga tcaaatcata ttatgatgag aaggaacacc
    59161 ttggatttgc ttacgcttat cagcttcctg gaatgaacaa agtcttgcaa gctggtggtc
    59221 gagtgattcg tggcatggcg gatcaaggaa tcgttgtatt ggcagatcaa cgattttcta
    59281 gtttttctta tcgacgttta ttcccacaac attggcaaac agcaagagaa gtacgaagtg
    59341 ttggggaatt ggaagaagga atacagcgtt tttggttatc aaaagaagca gtaaatcaaa
    59401 aaaacgagga atgacagacg tcagcctcgt tttttgatta acgatagtta gtgaattgca
    59461 actcgattgg taagtcaagt ttacgtaata aagcaatcac agcttgtaga tcatcacggt
    59521 tcttacctgt cactctaatc tgatcttctt ggatttgtgc ttttactttc agtttttcat
    59581 ttttgatagc tgtagtgatt ttcttcgcgt tttcacgatc gattccgttg accaaatcgg
    59641 cagtttgtcg agctttgcca cctagagcgt gttctgattc ggaaaaatgg atatttttga
    59701 ttggcacatt tcttttgatc agcttaccaa ataatacatc tttgatctgt tcgattttga
    59761 aatcatcatc gctcaataca actagattgc tgttctctaa tttgatttct actgtagaat
    59821 ccttgaagtc aaagcgattg gacaattctt ttgttgtttg ctgaatcgca ttttttactt
    59881 cttctgtatt gacttctgat acaatgtcga agctagcatc ttttgccata ggaaccctcc
    59941 tgatatacat ataaatattt accatacatc tatcttatca taggattagg gcagattctt
    60001 acctgtttgc taaaattctt ctcatttgag ttcgaagtat tttattttaa tagataaact
    60061 gttattctta catttggaat accaaagtag gggagaggtg cttgtgagta aaattatgac
    60121 tgcgtatcat aaaaatgaaa gattaaagaa aatagtggtt attttgataa ccggattgac
    60181 cgctgcggtc gctttaaatt attttttgat tccagccaat gtcttttctg cagggatgaa
    60241 tgggatcgct caaatcattt cctcgctttt atatgaatgg tttcatatca agatagacac
    60301 aggaacattt atttttcttt tgaatatccc tgtgtttgta ttagggtttc tcaaagtagg
    60361 aaaacgagca acgatcctta gttttgtgaa tgttgtatgt gtatcggtga tgacgatgct
    60421 gcttccagtc ggacagatca ctgataatat tttgatgaat gccctagtag gcggtgtttt
    60481 attaggtctt ggcgtaggat tatctttaaa aatgggcttt acaactggtg ggatggatat
    60541 catctctctt gtactttcac aaacaactgg aaaaacagtc ggaaactaca tgctgattct
    60601 aaatggtatc attgtagtca tcgcaggctt cttctttaac tgggaaagtg ccttgtatac
    60661 gattatttcc atttatgcaa tgactcaagt aattgatgcc atccacacga gtcatcaaaa
    60721 agtaacagca atgatcgtta ccgtcaagcc agaagaggta acagaagaac tagcaaaacg
    60781 gatggtacgt ggaatgacat tactgccgtc tgtcggtggc tattcaaaaa aacaagggaa
    60841 catgatcatg atggtcatca cccgctacga aatgtatgat cttgatcaga tcgttcatga
    60901 aatcgacgac gatgcattta tcaatatttt accaactcat tcagtatttg gccggtttgc
    60961 aaatgaaaac gaacaaaaaa tgtatcgaac aactggcgta ttgccagaaa taaaaaatcg
    61021 ttcacaaaaa aaatgaggaa tcaggtttat tttagaataa atttaatcta ttacatctaa
    61081 taagaacatt tttataaatt taaacgagaa tcttacttct aaattctcat ttacaagcag
    61141 ctaaaaaaag agtatgataa ataaaaatcg tgttaaggag tgtcgtacga tgagcgaagc
    61201 aaaccaagaa cggtctggac aagaaataga actaatcaaa gaacgtattc ttgctgcctt
    61261 ggaaatggtg attgatcctg aattaggaat cgacatcgtc aatttaggct tgatttatga
    61321 tatagaattc aatcctgaaa atggagaaac agtcattaag atgactctaa caactatggg
    61381 ctgtccatta gcagatattt taacagaatc tatccatgac gcattaaaag aagtgccaga
    61441 agtaacgaaa gcagaagtca aactagtctg gtatccagca tggacgacag ataagatgag
    61501 ccgttatgca cggattgcgt tggggatacg atagaaatct tggctgacta gctatgagaa
    61561 gcaaatagga aaaacaaaaa agccttttat cctttctaga agaaaagaga taaaaggttt
    61621 ctttgtttat aaaattttgt ctgctatatt ataagttaat taaataaatg tgttctattg
    61681 ataaaggaca ttcaaatccc tttccttttg gaatccttct tttttacatc agtttgattt
    61741 tcgtatagta ttctttctca tcttcacgat aaatcgcagc cccatttttt tgttgtgctt
    61801 taagactgcc aagatttttt ttatttacag acaaaagtgc ctcatcaatg ccgatttttt
    61861 ttgcctcttg tagtactaat gctaatcctt gtgtggcata tcctttttta cgataattag
    61921 gggagatgcc gtaaccaata tggccagcgc cgtttcttaa tgtttcgttc agtttatggc
    61981 gcaaattaaa aattcctaca tactcatgtt catcactttc taaaataaag acggttgcgg
    62041 gaacgtaact ctcaggaaga tcgattccta aagacgtttt ttcgcatttt tttacataat
    62101 ctaaaaattc agcaaatgta tagccataag cattgttgat aaatccactc tcatttggtc
    62161 caaagctagt ttgtagctga taagctttct ttaaatctgc ttcccatagt ttgatcagtt
    62221 tcatcgtatt cacctcttta agtaacctta ttcatttgtt ttctactata gcactattgt
    62281 tgtaaatagc catgcctatt tttatttatt gtgtcatatc gtattaagaa tcatcgaaca
    62341 atacaaatat gaattacaat aaaaaaatta tttgaagttc gttcattcgt tacttatgat
    62401 ctctagaaga aagaaaacgt gaattacttc agcgtctaga attccagaag gatagtaatc
    62461 aacagaagaa ataatcaata tccaaatgaa agaagatagc tgaatcgaga aaaagaggaa
    62521 aaaagaaatg aagaaaaaaa taaaatagtt actaatccca gagtcgttcg tttaacattg
    62581 aaatcgatca tatggataaa acagattttt tcaccaaccc tcaatagatg atggagttgc
    62641 tcggatgaat tatttgtgta gatggcataa gggagggcgt acagaaatga aaaagaacaa
    62701 aaatatgttc ttgacaaaaa acacatgtaa tcctaaacta aaagatgtga tgcggctatg
    62761 gcggaattgg cagacgcgct agcttcaggc gctagtgagg gtgacctcgt ggaagttcga
    62821 gtcttcttag ccgcataatt tgttttttta taaagagaag gtacacctgg agcgatgaga
    62881 aagaaaataa catattatct aaaagaatat aggaaaaatt ttaaacaacg gaaccagatt
    62941 caaataaatc aaaaaaaatt tgtgtgaagg tgggacagtt taaaacctaa tagattgatt
    63001 caaatagtag atttatagat tctgatacaa gaaatactgg caaacgatag ttaaggaata
    63061 aagactaaac ttaaactcaa aaagaggtca tgcgaaagaa taaatcgcat gacctcttac
    63121 tatattttga atgtgtttaa acgttcaaat aatgaatctt agtaccaacc gtttgcttcc
    63181 cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg ttctgctact
    63241 ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa ttggtaacgg
    63301 ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg tgcgatccat
    63361 tcttttgcag agcttgtact tgctggagta gcagcagttt ctgttacagc aggagcaggt
    63421 tgtgtttcag caacaggcgc tgcttgtgtt tccgtttgaa ccgtttcttg tacaacaggt
    63481 tgttcagctt gaacaggttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
    63541 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAYYAO010000004        60359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000004 DAYYAO010000000
VERSION     DAYYAO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.004"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..889)
                     /locus_tag="ACHARI_000187"
     CDS             complement(41..889)
                     /locus_tag="ACHARI_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8441271.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1113..3053)
                     /gene="thrS"
                     /locus_tag="ACHARI_000188"
     CDS             complement(1113..3053)
                     /gene="thrS"
                     /locus_tag="ACHARI_000188"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8441272.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3390..3569)
                     /locus_tag="ACHARI_000189"
     CDS             complement(3390..3569)
                     /locus_tag="ACHARI_000189"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8441273.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3616..5439)
                     /locus_tag="ACHARI_000190"
     CDS             complement(3616..5439)
                     /locus_tag="ACHARI_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8441274.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5717..7996
                     /gene="helD"
                     /locus_tag="ACHARI_000191"
     CDS             5717..7996
                     /gene="helD"
                     /locus_tag="ACHARI_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8441275.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8089..8736)
                     /locus_tag="ACHARI_000192"
     CDS             complement(8089..8736)
                     /locus_tag="ACHARI_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441276.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8915..9634)
                     /locus_tag="ACHARI_000193"
     CDS             complement(8915..9634)
                     /locus_tag="ACHARI_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8441277.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9765..10916)
                     /locus_tag="ACHARI_000194"
     CDS             complement(9765..10916)
                     /locus_tag="ACHARI_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8441278.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11133..11873)
                     /locus_tag="ACHARI_000195"
     CDS             complement(11133..11873)
                     /locus_tag="ACHARI_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8441279.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11877..12200)
                     /gene="rsfS"
                     /locus_tag="ACHARI_000196"
     CDS             complement(11877..12200)
                     /gene="rsfS"
                     /locus_tag="ACHARI_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8441280.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12257..12850)
                     /gene="yqeK"
                     /locus_tag="ACHARI_000197"
     CDS             complement(12257..12850)
                     /gene="yqeK"
                     /locus_tag="ACHARI_000197"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8441281.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12834..13484)
                     /locus_tag="ACHARI_000198"
     CDS             complement(12834..13484)
                     /locus_tag="ACHARI_000198"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8441282.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13494..13805)
                     /gene="yhbY"
                     /locus_tag="ACHARI_000199"
     CDS             complement(13494..13805)
                     /gene="yhbY"
                     /locus_tag="ACHARI_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8441283.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13821..14930)
                     /gene="yqeH"
                     /locus_tag="ACHARI_000200"
     CDS             complement(13821..14930)
                     /gene="yqeH"
                     /locus_tag="ACHARI_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8441284.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14927..15454)
                     /locus_tag="ACHARI_000201"
     CDS             complement(14927..15454)
                     /locus_tag="ACHARI_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8441285.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15634..16419)
                     /locus_tag="ACHARI_000202"
     CDS             complement(15634..16419)
                     /locus_tag="ACHARI_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8441286.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16412..17281)
                     /gene="accD"
                     /locus_tag="ACHARI_000203"
     CDS             complement(16412..17281)
                     /gene="accD"
                     /locus_tag="ACHARI_000203"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8441287.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17282..18661)
                     /locus_tag="ACHARI_000204"
     CDS             complement(17282..18661)
                     /locus_tag="ACHARI_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8441288.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18669..19088)
                     /gene="fabZ"
                     /locus_tag="ACHARI_000205"
     CDS             complement(18669..19088)
                     /gene="fabZ"
                     /locus_tag="ACHARI_000205"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8441289.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19113..19589)
                     /gene="accB"
                     /locus_tag="ACHARI_000206"
     CDS             complement(19113..19589)
                     /gene="accB"
                     /locus_tag="ACHARI_000206"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8441290.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19592..20827)
                     /gene="fabF"
                     /locus_tag="ACHARI_000207"
     CDS             complement(19592..20827)
                     /gene="fabF"
                     /locus_tag="ACHARI_000207"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8441291.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20846..21583)
                     /gene="fabG"
                     /locus_tag="ACHARI_000208"
     CDS             complement(20846..21583)
                     /gene="fabG"
                     /locus_tag="ACHARI_000208"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8441292.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21595..22512)
                     /gene="fabD"
                     /locus_tag="ACHARI_000209"
     CDS             complement(21595..22512)
                     /gene="fabD"
                     /locus_tag="ACHARI_000209"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8441293.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22619..22843)
                     /locus_tag="ACHARI_000210"
     CDS             complement(22619..22843)
                     /locus_tag="ACHARI_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8441294.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22896..23858)
                     /locus_tag="ACHARI_000211"
     CDS             complement(22896..23858)
                     /locus_tag="ACHARI_000211"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8441295.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23868..24308)
                     /locus_tag="ACHARI_000212"
     CDS             complement(23868..24308)
                     /locus_tag="ACHARI_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8441296.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24614..25579
                     /locus_tag="ACHARI_000213"
     CDS             24614..25579
                     /locus_tag="ACHARI_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8441297.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25636..25866)
                     /locus_tag="ACHARI_000214"
     CDS             complement(25636..25866)
                     /locus_tag="ACHARI_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8441298.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25952..26839)
                     /locus_tag="ACHARI_000215"
     CDS             complement(25952..26839)
                     /locus_tag="ACHARI_000215"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8441299.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26928..28151)
                     /locus_tag="ACHARI_000216"
     CDS             complement(26928..28151)
                     /locus_tag="ACHARI_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8441300.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28168..29214)
                     /locus_tag="ACHARI_000217"
     CDS             complement(28168..29214)
                     /locus_tag="ACHARI_000217"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8441301.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29378..31105)
                     /gene="ptsP"
                     /locus_tag="ACHARI_000218"
     CDS             complement(29378..31105)
                     /gene="ptsP"
                     /locus_tag="ACHARI_000218"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8441302.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31105..31371)
                     /locus_tag="ACHARI_000219"
     CDS             complement(31105..31371)
                     /locus_tag="ACHARI_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8441303.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31850..34084
                     /locus_tag="ACHARI_000220"
     CDS             31850..34084
                     /locus_tag="ACHARI_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8441304.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34232..34552
                     /locus_tag="ACHARI_000221"
     CDS             34232..34552
                     /locus_tag="ACHARI_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8441305.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34757..34981)
                     /locus_tag="ACHARI_000222"
     CDS             complement(34757..34981)
                     /locus_tag="ACHARI_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8441306.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35179..36759)
                     /locus_tag="ACHARI_000223"
     CDS             complement(35179..36759)
                     /locus_tag="ACHARI_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8441307.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37160..38536)
                     /locus_tag="ACHARI_000224"
     CDS             complement(37160..38536)
                     /locus_tag="ACHARI_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8441308.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38664..39752)
                     /locus_tag="ACHARI_000225"
     CDS             complement(38664..39752)
                     /locus_tag="ACHARI_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8441309.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39924..40358
                     /locus_tag="ACHARI_000226"
     CDS             39924..40358
                     /locus_tag="ACHARI_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8441310.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40436..40969)
                     /locus_tag="ACHARI_000227"
     CDS             complement(40436..40969)
                     /locus_tag="ACHARI_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8441311.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41063..42241)
                     /gene="mutY"
                     /locus_tag="ACHARI_000228"
     CDS             complement(41063..42241)
                     /gene="mutY"
                     /locus_tag="ACHARI_000228"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8441312.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42234..43031)
                     /gene="recX"
                     /locus_tag="ACHARI_000229"
     CDS             complement(42234..43031)
                     /gene="recX"
                     /locus_tag="ACHARI_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8441313.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43138..44520
                     /gene="rlmD"
                     /locus_tag="ACHARI_000230"
     CDS             43138..44520
                     /gene="rlmD"
                     /locus_tag="ACHARI_000230"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8441314.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44694..45272)
                     /locus_tag="ACHARI_000231"
     CDS             complement(44694..45272)
                     /locus_tag="ACHARI_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8441315.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45594..46064
                     /locus_tag="ACHARI_000232"
     CDS             45594..46064
                     /locus_tag="ACHARI_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8441316.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46172..47398)
                     /locus_tag="ACHARI_000233"
     CDS             complement(46172..47398)
                     /locus_tag="ACHARI_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8441317.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48152..48352)
                     /locus_tag="ACHARI_000234"
     CDS             complement(48152..48352)
                     /locus_tag="ACHARI_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8441318.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48486..49094)
                     /locus_tag="ACHARI_000235"
     CDS             complement(48486..49094)
                     /locus_tag="ACHARI_000235"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8441319.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49179..49649)
                     /locus_tag="ACHARI_000236"
     CDS             complement(49179..49649)
                     /locus_tag="ACHARI_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8441320.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49697..50611)
                     /locus_tag="ACHARI_000237"
     CDS             complement(49697..50611)
                     /locus_tag="ACHARI_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8441321.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50757..51560
                     /locus_tag="ACHARI_000238"
     CDS             50757..51560
                     /locus_tag="ACHARI_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8441322.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51738..52685
                     /locus_tag="ACHARI_000239"
     CDS             51738..52685
                     /locus_tag="ACHARI_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8441323.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52753..53046)
                     /locus_tag="ACHARI_000240"
     CDS             complement(52753..53046)
                     /locus_tag="ACHARI_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8441324.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(53074..53739)
                     /locus_tag="ACHARI_000241"
     CDS             complement(53074..53739)
                     /locus_tag="ACHARI_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8441325.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53879..54511)
                     /locus_tag="ACHARI_000242"
     CDS             complement(53879..54511)
                     /locus_tag="ACHARI_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8441326.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54518..55585)
                     /gene="liaS"
                     /locus_tag="ACHARI_000243"
     CDS             complement(54518..55585)
                     /gene="liaS"
                     /locus_tag="ACHARI_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8441327.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55582..56313)
                     /gene="liaF"
                     /locus_tag="ACHARI_000244"
     CDS             complement(55582..56313)
                     /gene="liaF"
                     /locus_tag="ACHARI_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8441328.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56482..56961)
                     /gene="greA"
                     /locus_tag="ACHARI_000245"
     CDS             complement(56482..56961)
                     /gene="greA"
                     /locus_tag="ACHARI_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8441329.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57097..58272)
                     /gene="mltG"
                     /locus_tag="ACHARI_000246"
     CDS             complement(57097..58272)
                     /gene="mltG"
                     /locus_tag="ACHARI_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8441330.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            58476..59645
                     /locus_tag="ACHARI_000247"
     CDS             58476..59645
                     /locus_tag="ACHARI_000247"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8441331.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(59912..>60359)
                     /locus_tag="ACHARI_000248"
     CDS             complement(59912..>60359)
                     /locus_tag="ACHARI_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441332.1"
                     /translation="NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDG
                     QVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVK
                     SESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        17531 a        12648 c        10168 g        20012 t
ORIGIN      
        1 ttgttattct aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat
       61 attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca
      121 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
      181 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      241 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      301 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      361 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      421 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      481 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      541 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      601 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      661 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      721 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      781 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      841 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      901 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
      961 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
     1021 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
     1081 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1141 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1201 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1261 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1321 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1381 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1441 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1501 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1561 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1621 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1681 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1741 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1801 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1861 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1921 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     1981 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     2041 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     2101 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2161 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2221 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2281 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2341 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2401 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2461 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2521 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2581 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2641 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2701 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2761 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2821 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2881 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     2941 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     3001 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     3061 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3121 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3181 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3241 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3301 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3361 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3421 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3481 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3541 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3601 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3661 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3721 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3781 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3841 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3901 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     3961 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     4021 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     4081 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4141 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4201 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4261 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4321 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4381 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4441 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4501 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4561 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4621 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4681 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4741 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4801 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4861 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4921 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     4981 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     5041 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     5101 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5161 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5221 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5281 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5341 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5401 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5461 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5521 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5581 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5641 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5701 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5761 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5821 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5881 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     5941 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     6001 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     6061 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6121 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6181 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6241 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6301 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6361 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6421 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6481 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6541 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6601 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6661 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6721 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6781 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6841 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6901 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     6961 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     7021 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     7081 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7141 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7201 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7261 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7321 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7381 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7441 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7501 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7561 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7621 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7681 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7741 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7801 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7861 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7921 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     7981 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     8041 atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata
     8101 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
     8161 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
     8221 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
     8281 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
     8341 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
     8401 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
     8461 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
     8521 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
     8581 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
     8641 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
     8701 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
     8761 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
     8821 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
     8881 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
     8941 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
     9001 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
     9061 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     9121 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     9181 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     9241 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     9301 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     9361 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     9421 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     9481 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     9541 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     9601 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     9661 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     9721 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     9781 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     9841 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     9901 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     9961 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
    10021 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
    10081 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
    10141 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
    10201 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
    10261 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
    10321 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
    10381 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
    10441 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
    10501 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
    10561 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
    10621 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
    10681 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
    10741 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
    10801 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
    10861 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
    10921 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
    10981 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
    11041 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
    11101 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
    11161 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
    11221 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
    11281 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
    11341 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
    11401 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
    11461 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
    11521 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
    11581 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
    11641 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
    11701 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
    11761 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
    11821 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
    11881 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
    11941 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
    12001 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
    12061 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
    12121 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
    12181 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
    12241 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
    12301 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
    12361 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
    12421 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
    12481 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
    12541 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
    12601 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
    12661 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
    12721 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
    12781 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
    12841 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
    12901 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
    12961 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
    13021 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
    13081 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
    13141 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
    13201 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
    13261 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
    13321 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
    13381 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
    13441 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
    13501 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
    13561 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
    13621 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
    13681 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
    13741 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
    13801 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
    13861 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
    13921 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
    13981 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
    14041 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
    14101 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
    14161 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
    14221 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
    14281 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
    14341 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
    14401 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
    14461 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
    14521 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
    14581 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
    14641 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
    14701 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
    14761 gagacatctt gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
    14821 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
    14881 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
    14941 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
    15001 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
    15061 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
    15121 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
    15181 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
    15241 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
    15301 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
    15361 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
    15421 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
    15481 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
    15541 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
    15601 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
    15661 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
    15721 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
    15781 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
    15841 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
    15901 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
    15961 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
    16021 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
    16081 gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
    16141 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
    16201 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
    16261 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
    16321 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
    16381 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
    16441 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
    16501 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
    16561 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
    16621 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
    16681 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
    16741 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
    16801 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
    16861 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
    16921 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
    16981 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
    17041 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
    17101 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
    17161 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
    17221 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
    17281 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
    17341 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
    17401 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
    17461 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
    17521 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
    17581 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
    17641 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
    17701 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
    17761 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
    17821 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
    17881 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
    17941 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
    18001 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
    18061 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    18121 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    18181 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    18241 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    18301 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    18361 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    18421 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    18481 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    18541 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    18601 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    18661 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    18721 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    18781 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    18841 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    18901 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    18961 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    19021 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    19081 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    19141 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    19201 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    19261 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    19321 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    19381 attatttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc
    19441 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    19501 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    19561 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    19621 ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt
    19681 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    19741 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    19801 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    19861 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    19921 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    19981 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    20041 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    20101 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    20161 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    20221 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    20281 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    20341 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    20401 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    20461 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    20521 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    20581 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    20641 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    20701 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    20761 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    20821 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    20881 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    20941 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    21001 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    21061 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    21121 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    21181 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    21241 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    21301 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    21361 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    21421 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    21481 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    21541 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    21601 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    21661 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    21721 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    21781 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    21841 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    21901 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    21961 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    22021 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    22081 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    22141 tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    22201 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    22261 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    22321 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    22381 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    22441 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    22501 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    22561 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    22621 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    22681 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    22741 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    22801 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    22861 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    22921 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    22981 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    23041 tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc
    23101 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    23161 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    23221 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    23281 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    23341 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    23401 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    23461 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    23521 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    23581 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    23641 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    23701 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    23761 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    23821 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    23881 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    23941 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    24001 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    24061 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    24121 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    24181 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    24241 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    24301 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact
    24361 atatcttaac ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt
    24421 ttttaagatt ttactcattt aaaacaactg ataaatcagc atttataatt tcttttatta
    24481 gaaaaatcat ctatctcaca tattttgatt atcaaagtat tttctgtgaa cagaactata
    24541 gaattcccta aaacttgtcc attatttcaa aatttgttat agtcttttta ttcaaagcgt
    24601 aaaaggagat cctatgttaa aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta
    24661 tcttatgaaa gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa
    24721 tagagtaagc agctattttc atcagaataa acagcattca aaaaaagtat tacgtatgat
    24781 tcatcatata acagattttg atttcgtcgt agtagacacg gaattagatg ctttattgct
    24841 cgagtgtcaa ttgatccaac attaccgtcc tttttataac cgacaaatga attatttcag
    24901 taattataat tatgtacata ttaccaataa gggatttgta ttaacagata ctcctacggc
    24961 tcgaacatac gggccttttc gtctctataa aaaaatgccg tctattttgc gtataatgga
    25021 agaaacttat caaatgcctt ggttgtctga aattagtttg ttagctttgc gagtccagct
    25081 tcctgaccta caggaaatga cttttgaaca gaaaaaaaag gaactccaag ggctgtttca
    25141 aggccgaaat aaaaagctac tgacttactt gaaaaaaaga caacaacatt tcatctatca
    25201 gttgaatttc gaaaaagccg gtatgcttca aaaagatatc gaattagtta cttattttat
    25261 tagacggatc caggaacaaa agcagttttt aagaacacca tcgctcacct tcagcatgcc
    25321 attagcagca gatgagagtc agaaaaagca ctacctcgtt tgttatggtc aacttgcaga
    25381 aacaatgatt acctctggtg acgtttctcc tgacttctat tatgaaaaga aagaagctca
    25441 tttatctttg aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag
    25501 ttaccgcaaa aaattagaaa aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc
    25561 tgaaaagcaa ctaaattagt tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt
    25621 caattatcct aaaatttatt ggattaagtc atagatttcc atcgctacga tatcgatatt
    25681 atcaaattcg tattgctgat gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt
    25741 atcataagtt actgtacact tctctacgcc ttctttttca aaacggcgaa cttgcagttc
    25801 attatctgct gactcgacca tagcctccag tcgttttatg attgcgacta attgtgaatg
    25861 tgtcatcgga ggtcctcctt ttcttatttc tgttatattg tatcaaaagc atagcctact
    25921 tgcattgatt ttctctaaaa aaactcgttt tttatgattt tttttctggt atttttcctt
    25981 caggccacca aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca
    26041 actcttcata tagtttcaat gcttgggtag ttgctggcaa tacaagatcc attttttctg
    26101 cttcttctaa tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt
    26161 aatcttcttt taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac
    26221 ctgaaagcgt ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca
    26281 tttccgtcat tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct
    26341 gacctttccc agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca
    26401 gtacttgatc ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg
    26461 ctcctaagtc accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg
    26521 tttttgcaat tttttctgcc aggcttggtg tacttgtagt caaatcaact aaaatatgtc
    26581 ctgagatatc tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg
    26641 ggtaacctac cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg
    26701 cccagatagc tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag
    26761 ctacttcgtg tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc
    26821 cgataaaacc aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc
    26881 tagtatacgc tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat
    26941 gaatttaatt ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct
    27001 tcttcatgat aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta
    27061 gatgaaatat cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa
    27121 atataatcaa taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa
    27181 ggatgatcga acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct
    27241 gccatcgctt ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa
    27301 tctgccgctt tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca
    27361 aattcttcta actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt
    27421 cctacgataa ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc
    27481 aaggcttgga tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt
    27541 tcctgaagac ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt
    27601 ttgaattttt caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag
    27661 ctgcgtaatg tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca
    27721 aataatctag acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa
    27781 tgaaggtagt cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta
    27841 gctaccatct tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt
    27901 tctagttctt tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc
    27961 ttgaaagaaa taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca
    28021 ttaggatcgg tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt
    28081 aatgttttga tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag
    28141 ccgattttca tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt
    28201 gctagagact ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt
    28261 attgatactt cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg
    28321 attggtaatc tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt
    28381 cttgctatct tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt
    28441 gcttgctaaa gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa
    28501 tagatctgca cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag
    28561 gacattttca ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat
    28621 gggaacatga ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa
    28681 atcaatgatt cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg
    28741 agacagtttg aaatattctc tgaacttttg ttctttcatt ggatcagggt gatatttctc
    28801 taagtcgatc ccattggaaa tagcttgaat cggcacacgg ataccatagc tttccaacaa
    28861 ctgttttgaa taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag
    28921 atattttttt acgataggag aaagctggtt cgatctgata aaggaatttc ggaaatcttc
    28981 ctctgtagaa tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat
    29041 attatgacta ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc
    29101 tagtgtataa gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc
    29161 gattccagat tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg
    29221 tcccccttca cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta
    29281 tttttgtcta ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta
    29341 gctcttttac aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa
    29401 gttcaacgac ttcttccatc atatcgcatt ctttcaatgc acgatcagca agttcagcca
    29461 ttttcgctgt gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca
    29521 ttgagaactc atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca
    29581 tttctccaca catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga
    29641 tcaaacgtaa aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac
    29701 ggtctgctgc cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt
    29761 cttttgcaaa cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt
    29821 ggatcgtatc agatacttcc ttgccttcac ttatcaattt ttgtttttct tcttcaaaga
    29881 tcgcttttgc tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaaat
    29941 taccatgaac agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt
    30001 gttcagataa actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta
    30061 attgcagata tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt
    30121 ttccttccat accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga
    30181 agtctggaga atccatatac aggaattcag tacggtacag accaactgct tctccaccat
    30241 tattatggac accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct
    30301 tgccgtcagc tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg
    30361 cgtaatctgc acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta
    30421 catcgccttc gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt
    30481 cttttgtccc aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag
    30541 tacgtccacc aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat
    30601 cacttggagt caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg
    30661 gtaaagtaac gcctaataag tgcgctaaaa tacgtttagc aacgtcacga atatctgcag
    30721 cacgctcttg catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg
    30781 ttacttcttt tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt
    30841 gacctaccat ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg
    30901 cttcacctaa gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag
    30961 ctaatgcagc atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga
    31021 atgttaaatc cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg
    31081 caatcccttt tagcatttca accattattc agataagcct tctttcttca ttgtttcaac
    31141 gattgctgcc attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc
    31201 ttgacctaca cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt
    31261 atactctaag tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg
    31321 acgagcgtgg atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg
    31381 tctcctttaa gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat
    31441 cctaacaaat gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca
    31501 gttttcattc aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt
    31561 cttcacattt tataacaaac gtgtttattt cctttttctt gtcgctttct cttctcctga
    31621 cgtttatcat tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc
    31681 ctttttattt ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct
    31741 tttctacctc ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga
    31801 gatgtaaaat atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca
    31861 aaactgcggc aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac
    31921 acaactcgat tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct
    31981 gactaatatg gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc
    32041 catgtctcgc caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg
    32101 cggaaacaac aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg
    32161 agaatacgga atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg
    32221 acgcgatgac gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc
    32281 tgtcttgatt ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa
    32341 aatcgtagat ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt
    32401 cgtatcttta gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt
    32461 gattgaagag atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat
    32521 cgtcggtgct ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc
    32581 tttagctcga ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat
    32641 cgaaaaagac gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt
    32701 cgaagaaacg atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt
    32761 aaaatatact gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga
    32821 tcgcttcttg ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaactt
    32881 gacgatccaa atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca
    32941 aaaagtttta gcttctcgag aagaagattt cgaaaaagca gcttattatc gcgatcagat
    33001 caataaactt caaaaaatga aagaacgtca gctcactgaa gaggaaacac ctgtgattac
    33061 cgaaaaagat atggaaaaaa tcgttgaaca acggacaggt attccagtgg gtgaattaaa
    33121 agaaaaagaa cagacacaac tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg
    33181 acaagataac gctgtagatc gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa
    33241 caaacagaat cgtccaatcg gctccttctt atttgttgga ccaactggtg taggtaaaac
    33301 agagcttgcg aaacaacttg cctacgaact attcggttca caagattcaa tgattcgttt
    33361 cgatatgtct gaatatatgg aaaaacatag tgtctctaaa ttgattggtt ctcctccagg
    33421 ttatgtcgga tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa atccttacag
    33481 tcttgtcttg ttagatgaag tagaaaaagc tcatccagat gttttgcata tgttcttgca
    33541 aatcttagat gatggtcgat tgacagatgc acaaggaaga acggtcagct ttaaagatac
    33601 gatcatcatt atgacaagta atgccggaac tggtaaagta gaagcaaacg ttggttttgg
    33661 tgcggcacgc gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact actttacacc
    33721 agaatttctg aaccgttttg acgggatcat cgaattcagt gcattatcaa aagaaaactt
    33781 gatgacaatc gtcacactaa tgctagacga tgtcaaccaa atgctggcag cacagcaact
    33841 tcacatcgaa gttcctacga atgtcaaaga aaaattagtt gacttaggct atgatccatc
    33901 tatgggtgct cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg gtatcgctga
    33961 attttatctt gatcaccctt ctatccatga gttgaaagca aaactggata aagatggaaa
    34021 aatcatcgtt acttctaaac cagaacgttt ggctaaagaa tcagccgaag aaacggctga
    34081 ataaatccag ttttatagcg tgagacggta tttctttgag aaatgccgtc tcattctttt
    34141 ttcagaaaag tcaaaatact gttcatttta taaaaaagaa attataataa gagaaacaag
    34201 caattaaaaa tacataaaag aggtgagcgc catgaaattg gtaaatgtaa caaacagcca
    34261 ttcccgtctt gtattaaacc agcttgaaaa cactgatgcc catctagtca aggtctacac
    34321 agccggaaat accactattg tctacactga agcccctgaa cataatgaaa ttctgctgat
    34381 caatgacaaa cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt atttcttaga
    34441 taaaatcaaa gacgccgttt accacgagga tgaaatcaaa gtaatcgaat tggcaggcct
    34501 tgttgaaatc tcgattccaa aacatgaatt aggcgctaca ttagccgtgt aaacaaattt
    34561 tgaacataat aattaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
    34621 aacacgtata aacggcgaaa gcagaagcaa ctccttcgga aataagctga aattcacgaa
    34681 aattgctttt caaatttttg tgaattccag cttatttctt ggagttaaac gcttctgtct
    34741 cagcctcttt ttctttctag ctttttgatt tttctctttt cttttgtact tttctctttt
    34801 cattttcagt tctacgcgtg tctttatttt taactttttc ctctttatgg atgattgatc
    34861 gtactttttg tgcaatttgc tcgtttttgt cccagtcttt tacatctaat aatcgacata
    34921 atcgtaacat ctcttctttt ttctgttttt ttctttcacg tctttgccat tctgtttcca
    34981 tgtgtcacac tctcctttct ttaatcatat gtacgcaaaa taatttttat atttttttga
    35041 ttagacattt ctatgatttt aatatagtaa aaagagccgt cacggaaaat ccgtcacggc
    35101 tctaaacaaa atatgagagg ctgtttttgt ttggctattc ttattacttg atgcagggca
    35161 gcttgtgtca agacttcttt acatcaaact ttttaatttt acattgggat atttatccgc
    35221 aaaccagcgt tctgcaaatt ggttctcgaa taagaacaac ggttggtcga aacggtcacg
    35281 agccaagata ttgcgacttg aactcatctt ctcatccaaa tcttctggat cgatccagcg
    35341 agcgattttg tgtcccattg gagacatgat tacttcggcg ttatactcat ttaacatacg
    35401 atattggaat acttcgaatt gtagctgacc aacagctcca atgatatatt cttccgttag
    35461 atatgtttta taaagctgaa tcgctccttc ttgaactaat tgatagattc ctttgtggaa
    35521 ggatttttgt ttcatgacat tttttgcagt cactttcata aacagctctg gagtaaatga
    35581 agggagttct tcataagcaa cttttagttt tccttcgaac aatgtatccc caatctgata
    35641 attgcctgta tcatatacac cgatgatatc gcctgctact gctttttcga cgttttctct
    35701 cgaatctgcc ataaattgtg tgacattgct taatttcagt tttttatttg tacgttccaa
    35761 ccagacatcc attcctcgtt cgaatgttcc agaacagatt cggacaaaag cgattcggtc
    35821 acgatgggct ggattcatat ttgcttggat cttgaatacg aaacctgaaa attcttcctc
    35881 ataaggactt accatctgat catcttgtgt tttgtgtcca taaggagctg gtgccaattc
    35941 aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc aatgcagaac caaaaaatac
    36001 tggtgtctga tcgccacgag caattttttc ttgattaaat gcatctccag cctctttgat
    36061 tagttctact tcttctaaga cttgttcata taaggaattt tcagttagag gatgattttc
    36121 gggaagcagt ccatcttcat ccaattctac taatcgttca tcctgataat tttccggacg
    36181 ataaaactct acacgattat ggtagatatc ataaagacct tctagccctt tacccatacc
    36241 gattggccaa ttcatcggat aagattcgat atctaatagc tcttctaatt cttctaacaa
    36301 ttccaatggt tcacgaccat cacgatctag tttgttgata aaagtaaata tcgggatgcc
    36361 acgttttttt acaacttgga ataatttctt tgtctgtgct tcgatccctt ttgcactatc
    36421 gattaccatg accgcactat ccacggccat caaagtacga taagtatctt cagaaaaatc
    36481 ttcatgtcct ggtgtatcca agatatttac tcgtttccct tgataatcaa actgcataac
    36541 agaactagta acagaaatcc cacgttgttt ctcgatttcc atccagtctg atttggcaaa
    36601 attacctgtt tttttgcctt ttacggtccc ggcttgacgg atagccccac caaacaatag
    36661 taattgttct gtaatcgttg ttttcccagc atccggatgg gaaatgatcg caaaagttct
    36721 gcgattgtcc acttgttgct ttaattttgg attattcata acctaactcc atttcttcca
    36781 ttcaattatt tatattttct ctaggcacag attgtccgat ctcttaaaga aagacaattt
    36841 catatagggc tgtgacaaaa gccgtgtagc agtctctttt tccgagtaaa cggcgttcta
    36901 gagacagcct atcagcaata agcccaccca agccaaaaat acgaaaagct gtttttgctt
    36961 ggctgctctt attacttaat gcaggatagc tttttcacaa cctttgtcaa ctactacaac
    37021 tgaatcagta tagcatggat tcagcgaatg aaaaagccac caaaccataa gagtcaaata
    37081 tttttttgtc agctgactaa aagaaaagac gtgttgcttg tttattcaag caacacgtct
    37141 ttcataacca agtttccaat tatcttcttg gctcgtcatc ttcgaattct tttctgaagt
    37201 atcttcgttc ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa atgacacgga
    37261 ttttaaggat tcgagaacct tccatttctt cagaaaccag cgtaatatta tcgacatcaa
    37321 acgataactt ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa taaccagcca
    37381 tcgtatctac atcactcata tgcagatcac tgtcaaaagc ttcattgaac tcatcaatca
    37441 gcatacgtcc ttggatgata tattcgtgtt catcgatttt ttcataaaga ttctcaactt
    37501 catctgattc atcatcgatt tctccgacga tttcttctaa aaggtcttct agtgttgcta
    37561 acccaacgac gccgccatat tcatctaata aaatagccat ctgattctgc gtctttttca
    37621 tttcatataa cagatcatca ataaagaccg tttcaggaac gaataaaggc tcctgcatga
    37681 ttttcttgat atctaaatta tcaaagccga atttatgggc agcttttagt aaattctttg
    37741 tatgaaggat tccgactact ttatctttgt cttcgttata aacagggatt cttgaatagt
    37801 tttcggataa aacttcattc acattttctt ccacagtgtc gttgatatca atcataaatg
    37861 catccgttcg tgggaccatg acttctcggg caactttcgt atcaagagaa aaaacccctt
    37921 ggagcatttc taattcttca ttattcaata cgccttcgtt ttcaagcata taacgcattt
    37981 catctctggt catttttgaa tcttcatcat caaaagtcat cggcgtgata cgcgccaata
    38041 aacttgtgga agcagaaagc agccagacga agggcttagc tactacacca atcaagcgta
    38101 tcagccctga agtgaacttt gcgacttctt ctgatttatt taaagcaata cgtttagggt
    38161 acaattctcc aaaaacaatc gatacatagg tcaaaatagc cagcacaatg atatttgcaa
    38221 tattttttgc cgctgtgccg ccacctaata ccggagctaa ccgttccgat aaagtattag
    38281 ctagtgaagc accagacaaa atgttgacca aagtgattcc tacttggatt gtcgatagga
    38341 aattatttgg attctgcaat atttttaaaa gcttttgaga tttcacatct ccttcatcag
    38401 ctttttgctc gattcgattc ttattgatcg aaacaacagc aatttcggaa gctgctaaaa
    38461 aagcattgat caatgtcaaa acgatcaata ataaaatctg cgctaacagc gactgactct
    38521 cagggtcagc attcataatc gcattctctc cttataaaat taatttttag tttacgatag
    38581 aaaaaaacgt ggcactatag ccacgttaaa atggtatcac aaagcaattt tctttgcaat
    38641 aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa ttttcttctt gttttcattt
    38701 tgcttgtctt ctttgacgat tttgattata taggaaatga tcgtcttgca gattgcgtaa
    38761 aaaggaacac ctaagaagat accaagcaat ccggcaatat ttccagctac tagaagaacg
    38821 ataatgatcg ttaatggatg gattttcagt gatttcccaa ttacattagg ataaatgata
    38881 ttcccatcca actgttggac gattagcaca accacacaac aaagtaatgc ttttaccgga
    38941 tcattgaaga ctgttactag aaatgcaggt gctagtccta gataaggtcc taaatatgga
    39001 attaggtttg tcaatcccgc aatgacccca aataagaaag cataatccac accaattacc
    39061 atatatccta aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg tccgctaatg
    39121 taattagcca atgttttatt cagttgcccc aataactcca caatttggtt tcttcttttt
    39181 tctgggaaaa actgttggat gtttggcact agtttttctc cgtctttcaa catatagaac
    39241 aagatgaatg gtgctgtaat gattactacg gttgccgaag cgatcgttcc aacgatcgaa
    39301 cctaaactat tggatactcc actaaggaat tgctgaatga ttgtcccgta agaaatgtct
    39361 aattgctcaa aatatttatt gatatctact tccttgaaca aaggcaattc ggctacctct
    39421 tttaaccagc tttctacctg tttgatgaaa tcaggcatat tagatgccaa tgaggaaatt
    39481 tgctgtacta gacttgggat gacactaagt aaaatccaaa taattgctgc aactaaaagg
    39541 agcagcacaa ttgcgacagc ccatattctt ttgatttttg ctttcatcaa cagcttgaca
    39601 ataggattca ataaatagta aagaaagcct gcaatcagta caggtgcaaa taacgtggaa
    39661 aaaaatgtcc cgatcggttc gaaaataaag tttatttctg atgaaatcat gatcaatgca
    39721 gctatgatca ataattcaag cgaccaaaac attagtttag actgtctaag tttttcaaac
    39781 aatagatttc ctcctttctt tcttactagt atatattata ttcgttaaaa attcttttat
    39841 tcttttttta tttttgaaaa cttgccatac tttttatttg ttttattttc cagtatacta
    39901 tacgaaagga aggtggctag accatgaaag ttgtacaaac aaaagataca atgagcaata
    39961 tttacttaga tgctgtacgg atcaggcatc aagtttttgt agtcgaacag ggtgtgcctt
    40021 tgtctcgaga aatagacaaa gatgaagccc attgtatcca cttcgtcctg tactcagata
    40081 aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa atgaaattac
    40141 agcggatggc gattctttct gaatacagac accaagggtt aggaaaaata ttgattgaag
    40201 aagcagaaaa ctttgctaag aatcaaggct ataatacgat tttattaggg gcacagtcca
    40261 cagctgaaac tttttacgaa aaactaggat atacagctta cggcgatcct tttgaagatg
    40321 ctggcatgcc tcatatttat atgaaaaaga ctttataaaa atttatttcg aaaaaagcaa
    40381 aaaacagcaa gcttttgaga aaaatctctc actggcttac tgttcttttt tgtgtttact
    40441 tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat atagccttcc gagaacgggc
    40501 catctccatt attgatccag tcaaccagaa tcttcacatt ttcttttagg atgaagtcaa
    40561 tatcgttcgg gtaattcatc attttgctgt gcatctcata ttcatcgaca tccaatagtc
    40621 gtttctcgcc gtcaggaaat accttgatat ccaagtcata gtcgatatat ttcaaggcct
    40681 catcgtccag caaaaaaggt gaagctagat tacaataata ggaaaccccc ttttctctga
    40741 tcattgctat tacattgaac cagtattttt tatgaaaata aacaatcgct ggttcgcgcg
    40801 ttacccatcg gcgtccatca gattcagtga ctaatgtgtg atcattcacg ccgatcatag
    40861 agtgttcgct tgtttttaat accatggtat ctcgccacgt tcgatgcaat ttgccgtcgt
    40921 gcttgtaact ttggatcgtc acaaactctc cttcttttgg aactcccata gcaacccttc
    40981 cttaagcagc attttgtgct ttgtgtttgg gtgttcatta caccgacatc ataattatta
    41041 tatcaataaa aatgagcttt gcctagaaag gattcctatt gtttcgattt ttctctaact
    41101 gatctaccag tttcatttgg accttaggaa aaacgaccga atcaaatgca tcaggcttca
    41161 accacttcga agttttgttc tcggtaaatt cttgttctgc tccctcggtt gcccgtccgt
    41221 aaaaaaggag aacatgccat ttcagatgac taaaaatatg agtaacttct cctaaatgtc
    41281 gtgtctgcca aacaaagagt tcctgctttt caaatgggag attttgctct tcttctgcta
    41341 ctaggtcatc aaacagatcg atttctggct ttgtttccca ttcttttttc aatcgctcat
    41401 actcctcttg tgtcacttcc atcattggaa atgtccacat attcgccagc aatttttggc
    41461 tatctctttc ttcaaaataa tacgctccag aatgattttg aagagctgca ctgatataat
    41521 aaacgtcttt tggctttgct ttttttgttt ttacaggaaa actcgtttgg attcctcgtt
    41581 tgttagctaa acaaaaggct tggatcggac aagtctcaca ctttggagaa gttggtgtac
    41641 agatagctga acctagatcc atcattgctt ggttgaattc acctggatgc ttttcatcaa
    41701 tgattttccg catcgcttca tcaaagattt tcctgctaga agcttttgcg atgtctgctt
    41761 caatgcaaaa caatctgctg actactcgca tcacgttgcc gtcaactgca ggttctggaa
    41821 gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg tcctattccc ttcaatgagc
    41881 taatttcttc aggcgtttgc ggcatttccc catcaaattc tgacatgatc tgtttagcag
    41941 cagcttggat gttccgggct cttgagtaat aaccaaggcc ctcccatgct tttaaaagct
    42001 tttcttctgg agcatttgct agctcttcaa ttgtaggaaa ccattccatg aaacgataaa
    42061 aataatcgat cactgtgtct actctggttt gctgaagcat gatctcagag atccagatac
    42121 gataaggatc acgattatag cgccaaggca ggtttctttt ttcttgttca taccattgaa
    42181 tgaactgatc ttgaaattct tttgtttctt tatctgtcca tttttcccat tctttattca
    42241 ctttcctctt ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa tcctttttga
    42301 tataagcttt gctttacttt tagatttctt tttttaggtt caaaccttaa atttcttcgc
    42361 catagtttat ctccttgttt gactaaataa tcatattcta tttcttgatc tgcttcttgc
    42421 ggaagatcag ctatcgcttg ttggatcact tcttgagaaa agcctttcgt catcaatgtt
    42481 tgttggattt ttctcaataa ttctttttgg ctttttccgt gattttttcc aaacgctttt
    42541 tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg tatattggct gagcgcttgt
    42601 tcaatgattt caggtttcaa acctttttgc tgcatctgct gcgctaattt ctttggtcct
    42661 ttgtcactta agcgcatatt cgtgcgcaca taactttccc catagaccaa atcatcgaca
    42721 aggtccaatt cttttaagcg ggctacgatt ttatttcgat cttcttgaag gatttccttt
    42781 tcttttaagt atgttcggat ttctttttcc gatcgcaact gataacttaa gtaattcatt
    42841 gctaggtgaa aaccgaaatc agctccgcta ctcttcttga tctcttggat agtttcttcg
    42901 tctaattccc gtccttttaa taatcgatag cgtactaata catcttcaga tacttgtaaa
    42961 gcttctcctt cagataaatc tacgcgatag aaagggcctt tgcctttact gattctcgta
    43021 acggttaaca taaacatcct cctcgaacat atattctcta tcataccaca gaaaatcccc
    43081 gtaatcaggt attatatgtt agaataaacc acagaaaaaa gaaaaaggga gatacttatg
    43141 gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg tttaggcata
    43201 aatggtgaag gaatcggtta tttcaaacgg ttgatcatat ttgttccata tgcactacca
    43261 aaagaagaag tactagtgaa aatcacgaaa gcaactcctc gctatgcaga aggagaatta
    43321 ataaaagtca aaaaaacaag caaagatcgc gtagtggcac catgtcctgt ttactatgaa
    43381 tgcggtggct gtcaattaca acacttagct tatcaagctc aattggattt taaaaaggat
    43441 ctgcttttgc aagcattgga aaaattcaaa ccagcaggct ttcgatctta tcaacttttg
    43501 ccgacgatcg ggatggatga gccttggcat tatagaaaca aagcacaatt tcagttacga
    43561 aaaaataagc agactcaaaa aattgaagct ggtttatacg aagcaaactc tcatcagtta
    43621 gtccctataa cagactgctt ggttcaagaa cctcgaacac aaaaagtaat gaatactgtt
    43681 gttcaactat tgaataaatt tgatgcaccc atctatgatg aacggacaaa cagtggaatc
    43741 ttccgcacaa tcatggttag aatcggtgtt aaaactgggg agttacaagt tgtttttatt
    43801 actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa tgaacgactg
    43861 cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct cgtgatgggg
    43921 gatgaaacga tccacttatg gggaaaagaa gccattgaag aacaaatcaa tgaagtgact
    43981 ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga attgctttat
    44041 ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga tgcttattgc
    44101 ggagttggga cgatcggctt aagtgtagca aaacaagcca aagaagtacg gggaatggat
    44161 accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg aaagattagg atatcacaac
    44221 actcgatatg aagtcgggac agcagagcaa gttctgccta aatggttgaa tgaaggtttt
    44281 cagcctgatg gtatcatcgt agatccacca cgtacgggtt tggataacgc attgatccaa
    44341 tcattattga aataccctcc aaaaaaactt gtttatatct cctgtaatgt ttctacactt
    44401 gccagagatc tggttaattt aagtaaaaag ttcaagattg aatatttaca gtccgtcgac
    44461 atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga tgacgagact cgctgactaa
    44521 atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa taagaacagc caaacaaaaa
    44581 tagctcttca tgttttatga atactgtact tgtgaccgaa gagttaacag gaggacacta
    44641 tttatttaaa aaaagggttg tgacatagtt tttgtcacag ccctttttga ttattattgg
    44701 atattcacgt ctatcacttg ataaaatgca tttgtcgtat cgctgatatt ccatactgct
    44761 aaaatcacat tatatccttt tcgatcctgc ggaagtgtga tcgtttggtt gactgttttt
    44821 ggtggaacag ttgctttgtc gtctattttt gtgatcaatt caaaatcact aaatttcaaa
    44881 ggttgattag gattccaagt tggtttggta atgtagtaat cccaagtact tgtccgatgt
    44941 tgtgctgtta aattccaagt aatatttaat gttcctggat taaccatgtt tttatgccat
    45001 ctgttagatg tctgttcatc taaaggctca aagccgctga ctcctgcact agcaattttt
    45061 ccatcaatga atgtattttt ggtagcttcg atactttgcg gttcccattg tgctcttccg
    45121 acattttgat ttaagtttcc tgctgctgat gttcctaggt atgcacggct tcctggagaa
    45181 gtaatataac catgcgcaaa acctggaatg ctaaaaccga acaaccctaa actaactaac
    45241 ccaataaaca ataacgtact aatctttttc atgtagaaac ctccttcatt tgataaccac
    45301 attgtaaacg ctttattgta ttaacacaaa cattatgctt ttataatttt atgttgaagt
    45361 aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag tagacgagaa aagtacaaat
    45421 agaaaacatg agaatcaaat catatgatct ttttcaaaaa catcattcat ataaacaaat
    45481 gttcaacttt ttaataacaa caattatcaa tttttttctt acttcacaaa ctaaatacag
    45541 gtcttgctgt gagccttcat ggtaaactaa tattatagaa aggagttagt aaaatgaaga
    45601 tgataaaaga tattcgtttt tgggtttgtg ttataggaat tgtcatatta ggctttttat
    45661 cagggttgct tagcggaaat cccggaaaat attactattc tttgcaactt ccaccatttg
    45721 caccacctag ctggatattt gggccgatgt ggaccttact gtatatattg atgggtattt
    45781 ctctttattt gttacttaat cacaacaaca aaaaacagcg aaacaatctt gtcggtcttt
    45841 ttgtgattca gttcattttt aattttatat ggtctgctct attttttaat ttaagaaata
    45901 ttttcattgc agcaatcgat attacacttt tagttatttt cttgtcagtg cttatgtatc
    45961 agttgtggct gcatcatcgc ctggcaatgt ggctcatgat tccttattat ttatgggtac
    46021 tattcgcaac tctattgaat tattcaattt attttttaaa ctaagcgcta aaaagagacg
    46081 tcctgtaatt taacacatag aaaaagtgcc acctaagtta gattttttgt ctaatttagg
    46141 tggcactctt tattaaccaa actatttttt tctagtaccc acttgttgtt ccatactcat
    46201 tattctggtc ataaccataa ccattttgtt cctgtccagc gtaaggatca taactttgag
    46261 tatcttgact tggtgtatag tactgacttg tatcttctgt ttgctgataa taggtattcg
    46321 gatcttggta ctgatcatat gtgttttctg aataggaaga ataatcggtg tacccttcac
    46381 tattcgatcc atcaccgttt gtcgaatcgc catcttccga atcatcaaag aacatcattg
    46441 aagcattctc atttttcaaa ttaggtaatg tatcgcttgt ttttatattt aattgtttct
    46501 tcagttcatt ttggatcgaa agaagatcat tttttcctaa aatttgatag tagatatcat
    46561 tgatcatttg tccttcgcct tgcaactggt cttgtttgat tgtttcaaac gccggggtgt
    46621 agttcgtcgc tacatcaagc atgtcatccc aatctaagtc cgtcgtcaca ttgttctcaa
    46681 ctgcttttaa aatttttcta taattgctta cgccatctaa acttaaaact ttattgacga
    46741 ttttcgtcac tacttcccgc tgacgacgtt gtcgaccgat atcgccttct ggatcttcgt
    46801 gtcgcattcg agaataggct aaaccttttt ctccattcaa tgtttgttca cctactggta
    46861 catggacacc atccaaagta aagtcaattt tattattcac tttgatgcca ccgaccgcat
    46921 tgatcaaatc ttccatgcca tccaaattga tcgttacata atgatcaata ggaatatcga
    46981 gcagttgctc aattgaatcc atggccattt cgacaccacc aaatgcgtag gcatgattta
    47041 atttatctac cgttccataa cctacgatat tcgtataaat atctcggtcc aaactgatga
    47101 tcgttgattt tttttgtttc ggattaactg ttactaccat catcgtatca gaacgtcctt
    47161 gttccgttct tcctaatcca ccggtatcta aacctaatag caaaaccgaa aaaggttctt
    47221 tgtcatcaat gcttactctc tcagaacgtt tggaggatat tcgattagag tttttagaaa
    47281 ttttgtttac tgtttggctc gcttctccca taaatctgat tccgtatgct gataatccgg
    47341 caatcagcaa aacgaatacc cctaacaagg ttaatagaac tttttgccat cgcttcataa
    47401 tacacctctg tttcacattt tctaaaatat tgtctcatat aactgttcaa tcagcaaatt
    47461 cactttttaa tcataagaaa actttaaaaa acatttttgt gtaacgatta ttatttttta
    47521 aaatttctca aaattcttaa ctatcatacc ataccgtgaa aaagatcgaa agaccgaata
    47581 cacgcagttc gcaatcgttt tatctttttt tatgtatttt ttccagaaaa agcgaacaac
    47641 aaggtatttt ccaaataatc gtgcagtaaa atgatcgcac atacctttcc catcttttta
    47701 acagtctttg cctaccgttc agattatcat tcgatttcac gacaatttct tcctatgact
    47761 ggtccttttg ttaaacccat cttctattcg ttttatttcc agctcactgc atggctctta
    47821 aaatgactcg aactagtgat tgcattacca tcatctttta atggatagat atgctcctct
    47881 tcaaagtaaa acgattttct tttaaagtaa tagaattgac atggtaatga gtatgtatac
    47941 tagtggtttc acaaatggtc ttttattcaa catccaaaaa ctgaaatttc tttaaaatcc
    48001 attactaacg aagaaaatgt tccttgcttg ataattagtt aggacatgtc aagtgaacaa
    48061 atgcatttac ttagccaaaa aatctaaaaa cttcataaat agacaaaaag acacgactct
    48121 ctaagaagtc gtgtctttat tcaacaaatt attataattt agtcacttca gcagcttgag
    48181 gtccgcgtgc accttcaaca atcgtgaatt ctacttcttg accttcttct aggctcttga
    48241 agccgtctcc ttgaattgct gagaaatgaa caaatacgtc atctccgccg tctactgtaa
    48301 taaacccaaa tcctttttca ttgttgaacc atttaaccgt tccgtgttcc ataaatgtat
    48361 atcctccaaa aaagttcttg caagtaacct tgcaagaaca ttatacccaa tttagaaaac
    48421 gctagcaagc tttcgctctt ttattttcca aattattttt aagactgata tgacggagga
    48481 aatggttatt ttgctgcttc gaaacgtttg tttacttcat cccaattaat aacattccag
    48541 aatgctgcaa tataatctgg tcggacattt ttatatttca aataataagc atgttcccaa
    48601 acgtccaaac ctagaattgg tgtctttcct tccatcaatg gagaatcttg atttgcagta
    48661 gaggtaatag ctaattttcc attttccatt acaagccaag cccatccaga accaaatcgt
    48721 ccagcggctg cttttttgaa ttcttcttta aaagaagaaa aatcaccaaa agcttcatta
    48781 atcgcttctt ttatttctcc tgtaggttcg ccaccagcat ttggtgccat gatttcccag
    48841 aaaaatgaat ggttagcatg tccgccacca ttgttacgta cagctgtctt gatatctgtt
    48901 ggaatagcgt ccatatcaga taataattct tctattgttt tttcgcctaa ttctgggtat
    48961 ttctcaattg ctgaatttaa attcgtcaca taagtattgt gatgcttatc atgatgcaga
    49021 tgcatcgttt cttcgtcaat ataaggttcc aatgcgtcat atgcataagg tagatctggt
    49081 aatgtgtaag tcataaaaaa attcctcctc aatgttttct taataatgtc tacattacaa
    49141 agagtaacaa agagaaggaa attcttcaat tgttatgcct agtcgtcttt ttcactttga
    49201 cctacttctt cttcaaaaac ctcagtttct ttaaaaataa acaacttgct gaaaatatag
    49261 ttcgcaagga taacaataat ttgagtgatg attttcgcca catactcatt gctgttcata
    49321 acattgatca acaataccat tgcccccatg tccaatccgt aagaaaggat acggtagaag
    49381 aagaacttac cgcattcaat aagtgcatgt gtaaaattcg gtgattttga atggaatacc
    49441 caaagtttgt tcgttacaaa agcaaacaat acagaacaaa accacgaaat agaattggat
    49501 aaggccatac ttaaatcaaa atattggcgt gcgagagcaa aactaacaaa atttacaatc
    49561 gtagccaaac caccaaaaaa gagatagact agtatctccc aatatccttt tgcttctaaa
    49621 tatcctttta actgtcgata ctgcttcatg agccaaccta ccttattaaa aattcttatt
    49681 tatcatactc tatatttcaa taactttcat ctttatttaa aaaaattaat tttttttgat
    49741 cctttgcctt tgtctctgct tggatcgtca catgtgtaat accatatttt aattttaata
    49801 aagaaattat acgaggatat atcttttcaa tctcgcttag tagctgatct tcaaaatcaa
    49861 tatgtccgtc gaaaataatt atcttttcgc tatactgcca aatatggaca tgatgaacat
    49921 cctttacccc atcgattgct tcaatttctt gttccatttt ttcatagtcg atagcaggtg
    49981 cactttgtat caaaatagcg gcagcttttt tgatcaccat tatcgcttct atcaatatat
    50041 atatcgaaat gatcagtgta ataactggat cgatccagta gatatcgaaa aaacggatca
    50101 aaattgctcc gataatcaca cttaccgaag acagtgtatc acttaacaaa tgcaaataac
    50161 tagattttat attcaaactt tcatctgctt cttgtcgtaa taataatact gagaacaaat
    50221 tcgcgagcaa tccaataact gctactgtta gcatcaaatg actatttatc ttttctggtg
    50281 aagtgaaccg cttgaatgct tcaacggtta aaaacaagga taatatgatc aatatgaatg
    50341 agttgatgaa agcactcaat atttctaaac gctggtagcc atacgtacgt tttttatttg
    50401 cttcttttcg ggccattttt tgtgccccgt aagaaaaaat aattgccaat gtatcactaa
    50461 ggttatggaa agcatctgaa acaagagaca aactccctga cataatccct ccaataaatt
    50521 ctgataaagt gatcaccgca ttcaaaacca ctactccaaa tagttttttt gatgatctct
    50581 gtggctgtgt aggcactatt ccttcctcca ttttatttat attggatagt ctttagacaa
    50641 gaataacatt tttttgatat ttcaccaatc attcagctgt ttccttaaag cacttgtatt
    50701 tcacttgata cttcgctaga attgcaagtg atgacactac tagatgagga gtacttatga
    50761 caacaaaaca attaattatc agttccttta tacgattcaa agacctaaag gaggcgagaa
    50821 atgtcccatt ttggaaagcc attgtctatc ttttagtttt gagtataatt atggctttgc
    50881 caatctcttt tcaagttttt caagtcttaa acaacattca attggatggg caaaagattg
    50941 ctactcgcat tcctgatttt acaatccaaa acgggcagct tcaaaccgaa gaaaaagaag
    51001 gattcattta ccaaacaaac tccattatct tcacatttga tccagaggga aaacgaacag
    51061 aacaggacat ctctactgat ttgatgggga attttgttag tgtcgggatg ttaaaaaaca
    51121 aacttatcat agctcttcct aatacaggca cgacttctgc tttgttgaac aataaccaat
    51181 tagaacttcc ctacaccaat gaatctttaa aaaacttaac aggtaagcag ctccgttctt
    51241 ttttaagtga agcatctata ccactttgga taaaagtcct gacatttata ttttctattt
    51301 atcctagttt tctgaatctg ctgatcactt tgctatttac caacattgct gcttttctct
    51361 atgcacgctt tcgactaaca aaagctactt ttcttgattg tttaaaaaca ttgatctatt
    51421 caatttcatt gcctactata attgcaacaa tcttgatggt ctttcttcct tcttttgaca
    51481 ctagcgcctt cattgctttt gcaggaatct ttatttttgc tcaagctgtc aaaggatggg
    51541 caaaaatatc gatcaattaa gaaataaaga caactcgtta tttctacttc aatagaccct
    51601 tttagaggtt gtgacatttt tttgtcacag cccctttttt atttgaaaaa tatttctttc
    51661 tcaaaaaaca agcaaggcgc ttcaaagttc ttcttgtatg cgctacactt agagtaaaga
    51721 aaggcgggaa attaactatg tcagaaagaa tcatttatgc agatcgtcca tttcgtgatg
    51781 aatttatcga agaaatccat actatagcgc ctggttatat ctttaaaaca gaattacaac
    51841 cagaggatct acaaaatgta gagatcagtt tgggctggaa ctcagactac caagaaaaac
    51901 tattagcctc ttctaccttg aaatgggtcc aatctatctc agctggtgtt gatcagctac
    51961 cccttgagac tttttcaaag aaacatattt tactttcaaa tggtagtggc atccacagtg
    52021 aatcgattac ggagcatgtc ttaggaatca tccttgggta ttctcgtggt ctttttcaag
    52081 cacaaaaggc acagagtgac atgaaatggt taggttcatc ggtccattac caatctatcc
    52141 aagggaaaaa aatactaatc attggaactg gccatattgg aaaaatgatt gcaaaaaaat
    52201 gcaaagcatt agacatgaac tgctatggaa tcaatacatc cggtcatcca gcagaaggaa
    52261 tggaaaagac tttttcttta aatcaactaa aggaagttct tcctgaagta gatttcgtag
    52321 tcaatatctt acctctaacg gaggaaacaa ctgggctctt tgatcagcat atgtttgaac
    52381 actttgatcc aaaatctgta ttcatcaatg ttggccgtgg tgcttctgtt aagacacaag
    52441 atctggtgca ggctttaaat aaccagcaac tttcctttgc agcattagac gtttttgagg
    52501 aagagcctct tccacaagat catcctcttt ggaaaatgga caatgtactg attacttccc
    52561 acattgcagg tttgacacct gattttcaaa agaaattaat ggctattttt ttaaccaatc
    52621 tgaaaagcta ctttgccacc catgagcttc aaaccaatca agtaaaattg tctgcaggat
    52681 attaatagct gaaaacaata ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca
    52741 ccctctcttt ttttattgtt ttttaaagtg ataggttggc tcctccaggt tgggatcata
    52801 atcaacaagt aaatcatatc ctttaaagaa ccattcatct gcttcctcga taaaaaaaag
    52861 tgtcccatca atttccactt tctttacagg atgttctggc tgttcaactg acattccaat
    52921 cgaaaaacca tcatgaacct ctgttttgcc gtagactttt ccaaaaaaac gtattccgta
    52981 agtttctggc agatcaattt ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa
    53041 tttcatccca acaacctact ttctcttttc tttctattga ttcatcttat cgtctagaat
    53101 atccacatcg cctgtctctc caataaccag tagattatca ttttcacgaa catattcatc
    53161 tgctgcagga gaagcgataa ccgtttgatc tactcttcgt atggcgacta ctgttaagtc
    53221 aaaacgttgc ctgaaattta attcagctaa tgttttgttg aaaaatttag gattggagac
    53281 tcggatttct gctaaagaat atttatctga caactctagg taatctaaaa tattctttga
    53341 cactaaatta tgcgcaatcc ttattcccat gtctcgctcg ggatggacga cgcgatcagc
    53401 tccgatcttt tcaagtactc tggcatgata ctcattttgg gcttttgcta aaatatttgg
    53461 tacgcccatt tcctttacca tcagagtaac taagatactg gcttgaatat cttctccaat
    53521 agcgacaatt acatgatcga aatttcttat gcctaatgat cgaagtgtca tttcatcctg
    53581 tgcatttgcc acaacagcat gagtggctat attcatatat tcattgaccc gatcttcatt
    53641 actatcaata gccagtactt cttgaccagc ttcgatcagt gttcggcaga tacttccgcc
    53701 aaatcgtcca agacctacaa ttgcaaaatt ttgtttcacc gttttcctcc tttagtatct
    53761 tcttttctca gtataacaaa gcctttacaa aaacaaaagc aagcatcatt attgtaaaaa
    53821 agccaagaaa aataatagtt attttctcag cttttgatta tcctctttat catcgttttt
    53881 atttcaccaa accatgtttg aatgcataga tagcggcttg tgtacggtct tccacttcta
    53941 gcttagccaa tatattggaa acatgtgttt ttactgtttt cagtgtaata aacaattcgt
    54001 ccgctatttc ctgattactt tttccttcag aaatcagcat taaaatttca ttttcccgat
    54061 tcgttagttc ttcatgtaat acaggctcat ttcgccgact cattttttcc atcatttttg
    54121 ttgtcacttc tggttctaat actcgctcgc cacgttgcgt tgcccttata gcatcagcaa
    54181 tttcatgtgc ggtagaggtc ttcaacaaat accctgctgc acctgcttca atggcaggat
    54241 aaactttttc gtcatcaata aaactggtta cgataatgat tttagcttct ggccaatctt
    54301 ttaagattgc ttttgttgat tcgataccat ccatttcttc catgactaag tccattaaaa
    54361 tgacgtccgg tcgaagtgtc atcgccttct catagccttg tcgtccattt tcagcttctc
    54421 caattacttc aatgtcttct tgaatagata aataaaaaga gacgcctaag cgcaccattt
    54481 catggtcatc tactaataaa acttttatca tccttatcta tgcctccttc atcaaaggga
    54541 ccttgatttc tacacttgta ccttgacctt taaaactaat gatttttact gttccaccaa
    54601 taccagctac tcgttctttg atattgttca atccataact tcccgttttc gtttcattca
    54661 tattaaaccc tgttccgtcg tcaataatac gtaacaaaag attattgtct attttgtgca
    54721 aatatacctc taattcattg gctttagcat gtcttaatgt atttgataac aattcttgaa
    54781 cgattcggaa caagtgatcc tcaatggaac tagatagttt tacatcttct acatcccatt
    54841 tcagcgaaat ctggatttta ttttgcaatt ccttcaataa ctgctcgata ccttgcttta
    54901 aacttttctc ttctaaattg actggacgta gatgaagaag cagcgcacgc atctcagact
    54961 gggaagcgtt gatgatctct gctaccattt tcaactgctt gcgaaacatt tctggtgtct
    55021 cgcttttttc tgcttgctcc gtcaatgccg acatcatcat cattgctgca aacaattgtt
    55081 gcgaaactga atcatgcagc tcacgggcta atcgatgtct ttctaattct aaaatttctt
    55141 ctttggtttg tccatctaca tattggggac ggctcgttac aatttgtaat tcactagata
    55201 cttcaatcat tttttctttt atcttagtga tttccttatc aatatctttg atgtataaat
    55261 cgtccgatgc acttgggatc ggaagaagca acaatttgga ttcataattg ccagaagaaa
    55321 gtgcacgcag cttttcttcg attttcccgt attttgtctt ttgaataagt gataataata
    55381 gaaagacaat taatcctaca cctaaggaaa tggctaacaa atggaagata agcggaacat
    55441 ataataaacg tgcacgcagt aattctcccc accaatgact ttgattgctg gcataaaaat
    55501 aagtgaaaag tgaaaataga ataataagga aagccgcaat ccccgaataa acagctagca
    55561 tcgctctgga tatttttccc atcatattcg tatcacctcg acatctccaa tcaaggtatt
    55621 agtaataatc ttcaatctac gagggttttc atcataatca ctgctgtaga ttttgatttg
    55681 ttcatttttc aaagaaaatt gctcttcttc aaacaagaca tttcctacta atgtcgcatg
    55741 ctctaatcgg atggctacgc ccaaaggtac taagatccgt gttctgccta tcccttttct
    55801 tacaatcaca atattatcat cttttggcaa aagcgtattg cccaaatcta tgatagtatc
    55861 accagaaatg acggcgatgt tgatatcgtc ccattcatag acgtcgtttc caatacgttg
    55921 gttaccgaat aattgctgtc ttttccgctc attattatgt gtttttaatt gctctgtttg
    55981 gatcatcatg atctgtttct ttcgccaaaa agcatttttg gttaaatcta tccctgaaat
    56041 ctctacacct ttcagaccaa taaataaaac ggcaaataca gccatcatcc ataaagcagg
    56101 actgttgact aagctaaaaa aaatgaccaa acttcctaat atcaattgaa aattttgaaa
    56161 tttagtacga ggatgtttgc gcatagctag atatatattg aaaataccga atatcactaa
    56221 aagcaaaagt ccagtattgt ttacgatttg ccaaacagca aataacagca gtagtgcttc
    56281 aactaccaca aaaaaacgcc aagaactatt catcgctctt tctccctcct gacacattct
    56341 catttcatca tacctatttt tttaactgtt ggctatagga ctttagatgt aagatcgttt
    56401 gttgaaatct aaaatacaca aagagaaaaa gatctagagt gtatcatcag taagatgaac
    56461 tctctagatc tttcagtcag actacgcttg ttctactttg acgattttaa ctaacatatc
    56521 gccacctgga gtagcaatag ttacttcatc atttttacgt ttgccaatca atgcttgcgc
    56581 aataggagaa tcattggaaa ttttgccaga aaacggatct gcttcagcgc ttccgacaat
    56641 cgtgtactct tcttcttctc catctggtag ttcgatgaat gtaactgttt taccaattga
    56701 tacctcatct gcatctacac cgtcgttatc gatgatttga gcaaaacgaa tcatattttc
    56761 aattgttgtg attcttcctt caacaaatgc ttgttcatct ttcgctgatt catattcaga
    56821 gttttcggaa agatctccga aaccgcgggc aattttgatt cgttcaatga tttctccgcg
    56881 tttgactgtt tttagttctt ctaattcttt ttctaatttt tctttacctt ctagagtcat
    56941 tggataaact ttctctacca tgtgtaacgt ctcctttttt cgataaaata ccgcaaaagg
    57001 taatcttttg aggaatatag tccaaaaaag attaccacgc ttttcttcaa atgtaaatgt
    57061 ttttctaaaa atgatcaatg tctagagcct gtttttctaa gaattattta catatttttc
    57121 cactaattcc atatgttctt cataagtttt agcgaaataa acatttccag tgctcgtatc
    57181 tgctacgaaa taataatagt cattttctgc tggttctaat actgccttga ttgcctcttc
    57241 gcttggattg tcaaaaggtc caggtccata gccggtattg gtatacaaat tatatggtga
    57301 atctacagct gtatcttcat acgtcactaa ttctttgtgt tcacctaatg cgtataaaac
    57361 agagatatct gactgaagtg gcatatttgc tttgattcgg ttaaagaaga cttgtgcaat
    57421 gtttttacga tcattttctt ttacgccttc tttttcaact agagaagcta gagttaaaac
    57481 ttcttgtaca gtcaaatttt tctgcttgat agctgaataa tagttttgca tgaccgtatt
    57541 cgtcttgttc accatttcaa tcactagatc cttaagcgaa gtactcgtgt aataatcata
    57601 tgtggctggg aataagtaac cttccaaacg gtattttgtg ttctcagctt tagatgcact
    57661 ttccaacagt tctgggaatt tttggtgcaa ctcgttaaag aaagtctcat cattcactaa
    57721 atctaaaaat tctttcttgt ctttaccagt taccttcgct acccgttcag caatctgatc
    57781 gatatcgtac ccttctggaa tggcaatttt cccatctgca acttttgttg gctcagaagt
    57841 accgccttct tgcaactgtt tgccaatctc gtcaagagtc atattaggcg ctaactgata
    57901 atagccagct tgaaagccag ttaaattttt aaattttgta taataattga aaacaatccc
    57961 gcttttaata atattgtcct tctccaaaat ctcaccaatc tgcttgttag aagagccact
    58021 cgggatctcg acttggacta attggtcatc tgatttatct aaaggcttca acccgctatc
    58081 tacataacga tacacggtaa atccaagaaa tccgccaata atcaataatg taagagcaat
    58141 cactaaaatg atcttcctca caatcttatc ttctttttta cgcagatttc tccgcttgtc
    58201 gattatatct gctgtagctg ccttactggc ttctttttta tctttacttg ggtgttgttt
    58261 gtttcctgac aaaacaactc ctccttacta tcaagttcgt aactttattc attatacatg
    58321 atatttgaat tggcgtgaat gattttattt aaatattcta tcaaaaatgt aagtagtaaa
    58381 tccattacta taagattaaa ataaatgtta tcaagataca agctatttta gctttattct
    58441 ttctgtaaaa aagaaaggag actaaataaa ctattatgaa aaaaggaaaa catgctaaaa
    58501 aaagaaaata ttcattttta atgatcgttg tcgtgttgtt tagccttagt gcgttctcta
    58561 caggaactat tcttggatgg caattttaca ataatgaaca ctctcagata gttgcccatg
    58621 ctcaatcagc tcaaaaaaag ttgatactga ctaccaatga acaacagctc gttgaaaaaa
    58681 tcgatcaact attaagaaaa aatgattata ttggctcaat ttacgtaaag aaaaataatc
    58741 gcatcatcat ggaaaaaggc tatggctatg cgaataaact cacgcaacaa ccaaatgatc
    58801 cttctcttta ttatcagatt ggttcggttc aaaaagcatt gactactctt ttgattttaa
    58861 aacaagtcga gcgtgggcac ctttctttag atactaagct ggctcaattc tatccacaaa
    58921 ttgctggcag ccagcaaatt actatccgtg acttgctgta tatgcgctca ggcttgcacc
    58981 gaactgcttc tccaaccata ccgatgactg atgaagaagt cgttcaattt gcgattcagc
    59041 acttaaaatt tacagattat cagacctctc attatgattc attgaacttt accatattga
    59101 ctggaatttt gatcaaatta acaaatcaac cgtatgaaaa attattgacc gatgatatca
    59161 tcgaaccttt aggattacag catacaaaat tttatgacca agtcaaaaat tcatccaatc
    59221 atgcactatc ctatcaaatg tctaaagagg atgattactt taaaccactg acagaatctg
    59281 aaacagagat ccataacgaa ttagggactg gtaatatcag tatgtccgtc tatgatttaa
    59341 atacgctgtt tacacaagtt ttatctggaa aactagtttc caaagactta ctcttctcat
    59401 tatggcgtga aaatgcgaat ggacatccat atagtggtgg ggtatatagc ggagataatt
    59461 atatcctgtc gcaaggaaat atcgactctt ttgatacatc tgctatattt agcaaagatt
    59521 cacaggatgc tatcatcatg gaaagcaata taaaagctga caaaactatc aaactgccta
    59581 ctgctgatct aagaaaccaa atctatgacc tgatggaagg aactactaag ttggcaaata
    59641 gctgatatga tgaaaagata gcaaatacta cttctttgaa tagcatttgc ttaaaaaaag
    59701 tactcgacta aacgtaggaa agagtgtggg acaaatctaa aaatcaattt ttgctacaga
    59761 ctttaattcc aaataaacag aacagaaaga ggtgttcttc tttctgttct gtccaaatta
    59821 ttctaagatt tctctttacc aacattattt gacaaagggc ctattaaatc taaaatagta
    59881 tctttagatt gctaaaatat gtgctagcaa atcattcatg gcttgttctt atttttaatt
    59941 ttatgcaata aaaaagaaga acaattagcg tgattataga aataaataat gagattttag
    60001 aaaaaaatgt gtatgaatac ctgattgtta ctttatttat tccactttca ctttttactg
    60061 taacaaaagg acctccttgt tttagtactg tagcattttt tgaattaaca gtaaccgtta
    60121 atcctttata ccggaataca ggtaaaacta ttgttttgcc ttttgaagat ttaaacgtaa
    60181 atgtaatttc atctttattt ggagaaaagc tactataaat aacttgacca tcttctgtaa
    60241 cttgatggtt tttcaaaagc aattgatctt ttttatagcc tataggtaca taatcaaaat
    60301 tctcaaattc ttcaagttta ttttccattt gttcattaga ataagttcct tgaaaattc
//
LOCUS       DAYYAO010000005        60326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000005 DAYYAO010000000
VERSION     DAYYAO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.005"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..1412
                     /gene="nhaC"
                     /locus_tag="ACHARI_000249"
     CDS             45..1412
                     /gene="nhaC"
                     /locus_tag="ACHARI_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8441333.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1693..2712)
                     /locus_tag="ACHARI_000250"
     CDS             complement(1693..2712)
                     /locus_tag="ACHARI_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8441334.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2734..3741)
                     /locus_tag="ACHARI_000251"
     CDS             complement(2734..3741)
                     /locus_tag="ACHARI_000251"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8441335.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3734..4399)
                     /gene="pgmB"
                     /locus_tag="ACHARI_000252"
     CDS             complement(3734..4399)
                     /gene="pgmB"
                     /locus_tag="ACHARI_000252"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8441336.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4392..6686)
                     /locus_tag="ACHARI_000253"
     CDS             complement(4392..6686)
                     /locus_tag="ACHARI_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8441337.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6946..9111
                     /locus_tag="ACHARI_000254"
     CDS             6946..9111
                     /locus_tag="ACHARI_000254"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8441338.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9415..10248
                     /locus_tag="ACHARI_000255"
     CDS             9415..10248
                     /locus_tag="ACHARI_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8441339.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10350..12050)
                     /locus_tag="ACHARI_000256"
     CDS             complement(10350..12050)
                     /locus_tag="ACHARI_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441340.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12557..13717
                     /locus_tag="ACHARI_000257"
     CDS             12557..13717
                     /locus_tag="ACHARI_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8441341.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13733..14176
                     /locus_tag="ACHARI_000258"
     CDS             13733..14176
                     /locus_tag="ACHARI_000258"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8441342.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14659..15189)
                     /locus_tag="ACHARI_000259"
     CDS             complement(14659..15189)
                     /locus_tag="ACHARI_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8441343.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15294..15845
                     /locus_tag="ACHARI_000260"
     CDS             15294..15845
                     /locus_tag="ACHARI_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8441344.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15998..16471)
                     /locus_tag="ACHARI_000261"
     CDS             complement(15998..16471)
                     /locus_tag="ACHARI_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8441345.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16609..18081
                     /locus_tag="ACHARI_000262"
     CDS             16609..18081
                     /locus_tag="ACHARI_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8441346.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18078..18788
                     /locus_tag="ACHARI_000263"
     CDS             18078..18788
                     /locus_tag="ACHARI_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8441347.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19140..19424
                     /locus_tag="ACHARI_000264"
     CDS             19140..19424
                     /locus_tag="ACHARI_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8441348.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19741..20124)
                     /locus_tag="ACHARI_000265"
     CDS             complement(19741..20124)
                     /locus_tag="ACHARI_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8441349.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20126..20710)
                     /locus_tag="ACHARI_000266"
     CDS             complement(20126..20710)
                     /locus_tag="ACHARI_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8441350.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20757..22232)
                     /locus_tag="ACHARI_000267"
     CDS             complement(20757..22232)
                     /locus_tag="ACHARI_000267"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8441351.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22413..22724
                     /locus_tag="ACHARI_000268"
     CDS             22413..22724
                     /locus_tag="ACHARI_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441352.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22839..25175)
                     /locus_tag="ACHARI_000269"
     CDS             complement(22839..25175)
                     /locus_tag="ACHARI_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8441353.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25317..25985
                     /locus_tag="ACHARI_000270"
     CDS             25317..25985
                     /locus_tag="ACHARI_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8441354.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25988..27031
                     /locus_tag="ACHARI_000271"
     CDS             25988..27031
                     /locus_tag="ACHARI_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8441355.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27268..28149)
                     /locus_tag="ACHARI_000272"
     CDS             complement(27268..28149)
                     /locus_tag="ACHARI_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8441356.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28308..29036
                     /locus_tag="ACHARI_000273"
                     /pseudo
     CDS             28308..29036
                     /locus_tag="ACHARI_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
     gene            29192..29509
                     /locus_tag="ACHARI_000274"
     CDS             29192..29509
                     /locus_tag="ACHARI_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8441357.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29502..30110
                     /locus_tag="ACHARI_000275"
     CDS             29502..30110
                     /locus_tag="ACHARI_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8441358.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30165..30956
                     /locus_tag="ACHARI_000276"
     CDS             30165..30956
                     /locus_tag="ACHARI_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8441359.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            31094..31582
                     /locus_tag="ACHARI_000277"
     CDS             31094..31582
                     /locus_tag="ACHARI_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8441360.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31633..32505
                     /locus_tag="ACHARI_000278"
     CDS             31633..32505
                     /locus_tag="ACHARI_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8441361.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32681..33043
                     /locus_tag="ACHARI_000279"
     CDS             32681..33043
                     /locus_tag="ACHARI_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8441362.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33052..33804
                     /locus_tag="ACHARI_000280"
     CDS             33052..33804
                     /locus_tag="ACHARI_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441363.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33818..35620
                     /locus_tag="ACHARI_000281"
     CDS             33818..35620
                     /locus_tag="ACHARI_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441364.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            35897..36937
                     /locus_tag="ACHARI_000282"
     CDS             35897..36937
                     /locus_tag="ACHARI_000282"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8441365.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(36989..39133)
                     /locus_tag="ACHARI_000283"
     CDS             complement(36989..39133)
                     /locus_tag="ACHARI_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8441366.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(39490..40863)
                     /locus_tag="ACHARI_000284"
     CDS             complement(39490..40863)
                     /locus_tag="ACHARI_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8441367.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(40863..41258)
                     /gene="rbsD"
                     /locus_tag="ACHARI_000285"
     CDS             complement(40863..41258)
                     /gene="rbsD"
                     /locus_tag="ACHARI_000285"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8441368.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(41286..42203)
                     /gene="rbsK"
                     /locus_tag="ACHARI_000286"
     CDS             complement(41286..42203)
                     /gene="rbsK"
                     /locus_tag="ACHARI_000286"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8441369.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(42311..43324)
                     /locus_tag="ACHARI_000287"
     CDS             complement(42311..43324)
                     /locus_tag="ACHARI_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8441370.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(43405..44004)
                     /locus_tag="ACHARI_000288"
     CDS             complement(43405..44004)
                     /locus_tag="ACHARI_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8441371.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            44190..44891
                     /locus_tag="ACHARI_000289"
     CDS             44190..44891
                     /locus_tag="ACHARI_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441372.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            44901..47612
                     /locus_tag="ACHARI_000290"
     CDS             44901..47612
                     /locus_tag="ACHARI_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8441373.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            48063..49691
                     /gene="treC"
                     /locus_tag="ACHARI_000291"
     CDS             48063..49691
                     /gene="treC"
                     /locus_tag="ACHARI_000291"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8441374.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(49751..50932)
                     /locus_tag="ACHARI_000292"
     CDS             complement(49751..50932)
                     /locus_tag="ACHARI_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8441375.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            51184..52707
                     /locus_tag="ACHARI_000293"
     CDS             51184..52707
                     /locus_tag="ACHARI_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8441376.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(52776..53504)
                     /locus_tag="ACHARI_000294"
     CDS             complement(52776..53504)
                     /locus_tag="ACHARI_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8441377.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(53517..54797)
                     /locus_tag="ACHARI_000295"
     CDS             complement(53517..54797)
                     /locus_tag="ACHARI_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8441378.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(54938..55540)
                     /locus_tag="ACHARI_000296"
     CDS             complement(54938..55540)
                     /locus_tag="ACHARI_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8441379.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            55785..55991
                     /locus_tag="ACHARI_000297"
     CDS             55785..55991
                     /locus_tag="ACHARI_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441380.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            56303..57022
                     /locus_tag="ACHARI_000298"
     CDS             56303..57022
                     /locus_tag="ACHARI_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8441381.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(57103..57786)
                     /locus_tag="ACHARI_000299"
     CDS             complement(57103..57786)
                     /locus_tag="ACHARI_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8441382.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            58006..60192
                     /locus_tag="ACHARI_000300"
     CDS             58006..60192
                     /locus_tag="ACHARI_000300"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8441383.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        19233 a        10759 c        12216 g        18118 t
ORIGIN      
        1 ttttttcatg gcagggcctt ttcatttgaa ataggagatc gacgatgaat aaaaatattt
       61 ctttaaaaga aagtatcgct gtattgatcg tgttgttggc actcctagga gtactgatca
      121 tcggattcca gctctcgcca catatcccga tccttgcagc tttcatgtgt ttgttgtttt
      181 atggcagatt taaaaagttc acatgggatg agatccatga cgggatcatc aaaggaatca
      241 ctccagggat cattccaatc ctgatttttc ttttgattgg tgtcttagtt gcttcatgga
      301 ttttatctgg aaccatccct acgattatgg tttatggatt taaacttgtt tctgtgcgtt
      361 tctttttacc gacagcattt cttgtttgtg caattgtcgg agtgactgtc ggcagttcat
      421 ttacaacgat ttcgacgatt ggaattgctt ttttggggat cggtcattta cttggattca
      481 atgatgcaat gactacaggc gcagtcgttt ccggtgcctt tttagggaat aactgctcgc
      541 ctttatcaga tacgaccaac ttggcagcgg gtattggagg agttaatcta ttcgaacata
      601 tcgctggtat gcgttatacg gatttcccag cattcgtcct ttcgttgatt ttttatacga
      661 tattaggaca taacagtcat tcagccgatc tagctagtat caatgaaatg atccaaaaca
      721 tgaaagcgag cttttggatc tctccttgga cattgattcc actagttatt ttatttgggg
      781 gggcaatcaa aaaaattccg gctatcccta cattactagc gggatcgaca gctgctctcg
      841 ttctttcttt tatccatgag aaaagtttga cggtcggaaa agcagcagac atcttgatga
      901 atggttatgt cgcaaataca cctgatccaa agctagatga actgctttct cgaggcggga
      961 tcatgagtat gttaggttct gctagtttga ttttacttgc attggcatta ggtgggttac
     1021 tgatcaaata tctgatcgtt gataccatcg ttcaagaatt aaaagaaaag atggaccgac
     1081 cttctcgttt agtcgggttt actgccttga gctgtatcgg aatcaatctg atcgttggag
     1141 aacagtatct gtccatcatt cttccaggag aaacattcaa gcgttctttt gatgacgttg
     1201 gcttatcaaa aacctatttg acacgaacac tagcggattc aggtgcgacc gtcaattctc
     1261 tcgtaccgtg gggagtcagc ggcacattca ttatgggaac gatgaaagtt agcgcgttgc
     1321 attacttgcc attcgccttt ttctcgattc ttgcacctgt ctttacaata ttaggcggtt
     1381 ttttattgaa tcgtcgacgt gtaaaaaatt gaaggaagga aaaaagaggc tgggacagaa
     1441 gcgtttaact ccaagaaata agaagaaact caagaaaatt gctttttaaa tttttgtgaa
     1501 tttcagctta tttccgaagg agttgcttct gcttccgccg tttatacgtg tttagagcgt
     1561 gagacaaaag tgttctttac ttttgtccca cgctcttttt tacagataaa caatgtgtta
     1621 gaagtagtat cttcttttac ataaaaagct gaccatagga taaatggtca gcgtatttag
     1681 tgagtcagct atttagtatg ggttttctaa aggaacgact gtctctcttt tgatcaagcg
     1741 atgagggatg accaaacgta cgccactgct caattcttca tggatcaatt cgcgcagctt
     1801 ttgcatagcg acacgtccta attcggagat atcgatatcg atacttgtaa ggtaagggtg
     1861 tgttaatgtt gagaagatcg agttgttgaa gctaatgacg gagagtgtct ctggaatgac
     1921 atagccatac atttgcgcca actgcatcgt gcgaagggcg aagatatcgt caatgaccac
     1981 caatgctgtg gcttcgtttt ctttcaacgt tttctcaaag gacagataat cctccggatg
     2041 ctctagtact acactggata acgcaggaag accagccatc atcattgctt tttgataacc
     2101 gaaatatcgt tcgaaataca aactttcatg agtcgtgttt gtcacaaaca ggatattctt
     2161 atgtccattc tggatcaaaa attctgttgc ttgtttacct agaagctggt tatcattatc
     2221 tacataaacg atttcgttct catgatgata aggctgtccg atcaaagtga aagggatgtg
     2281 gttttggtat aaataatcaa tgacagtatc ttgactatct gaatacacta gaataaaacc
     2341 atctacttgt ttttgtaaat gcatacgctg gacgttctct aacagcaatt caaaattttt
     2401 agcagtagcg attgctgtag tcatatcata attccgcgct tcttcgttga ttgcttccat
     2461 gatctcaaga taaaatgggt taccgatccg ctcttttgaa tcaagagggg gtaagattac
     2521 gccgatggca ctggaaatgc gttttcctag attgcgggca gtcatgttag gaacataacc
     2581 tagttcatcc ataatttttc ggactttctt ctttgttgtc tcggaaatac taggatgatc
     2641 gttgatgaca cgagagacgg tcgaggtggc aacaccggct tttttcgcca catcttttac
     2701 ggtaatagcc atgataaaaa cacctttctc ttgttattgt gcagagaacc gataaatcgt
     2761 ctgactgatc cgttcttgac caggatggag gttgatcgat ccaaatttgg ggaaatgaac
     2821 gatatcaggg atttcttgcg gttcgattgc taatccgtag tttgaatgca tctgttttcc
     2881 attgacagag aaatcagctt cgaatccagt cgtagaaaat aataccatac ttttattaga
     2941 agttgctata gtcatttggc gaccagaatg cttctcgaat aattgtaagg aagttttgga
     3001 aagcctagga tgatgcaaaa gaaatacatc atctagtcca tctggataac atgcaaggat
     3061 ctcttgcagt gttggggatt ttctaaagtc gattggcagt ttttcttttt ttgtccaatt
     3121 tccagtaggc agtttttctt gatctaattc aagcacacca tctaaaaaga ccgttaactg
     3181 atgttcatag atgtctcttg ttccatttcc tgaaaggttg aagtaggcat ggttggtcgg
     3241 attgcagatg gtttcaacaa ggctactagc agaggttgtc atctctaatt cgttttttgt
     3301 taaacggtaa gtgatcgtag cagtgattgg tcctggatag gtagaaaatt catcttttaa
     3361 gtaaaagacc acacctattg atgatggagt tttgaaaact tctacttccc aaaattggaa
     3421 agaccagcct ttcgtgccac cgtggatatg gtgtgatccg cagtttttct ccaatctatg
     3481 ctcaccccaa gaagcatccc ggatccttcc ggctacaggt ccgacagttg ctccaaaaaa
     3541 gcttttgtct gttagaacat cttcgaaacg atcgtaagac aataaaacat tttctgattt
     3601 tccttctttg tctggtgcga agatttggtg caggcgggca ccgtaattga gaagaacgac
     3661 ttctaagtag tcgttcttca atgtgatttt ctttatttca gtctctggaa ctggtacgac
     3721 ggagatctct ggattatcca tggtcatgcc acttttcttt caagtaatcg atcgtaagaa
     3781 aagtcgtatc agggacaagc gggatctcct ctccaaggtc ttcagcacgt cccacgccaa
     3841 ttggttgagc gccacttgag aggatggctt ggataccggc ttgcgcgtct tcgatcccga
     3901 tacattcttc tgcttttagt ccgacagctt ttgctgcaag gaggaagata tctggcgcag
     3961 gttttccgtt tgttgcttct gccgggtcgg caatagcatc aaagtaaggt gtaagaccca
     4021 ttttttcaag taggaatggc ccgtttttac ttgcagaagc aagagcaatc ttgatttttt
     4081 cttcttttaa agaatccagt aaaggcacga ccccggggaa gacatctttt ggttcgatcg
     4141 tctggatcat ctctaaataa taatcgtttt ttcttttcgc taattctgca aattcttcct
     4201 ttgaaaaaga gtgttctttt ccgccataag ctaaaagcaa agagagtgag tcttctcggc
     4261 tgacgccttt tagctgttcg ttgaatgtgt ggtcgatcgt aatgccgatt tcctgaccaa
     4321 gctttcgcca agcacgataa tggaattcag cagtatcggt aatgacaccg tctaaatcaa
     4381 acaatacccc tttaaacatt tgcttcctcc tttcttagcg ttatttctag acgatccgct
     4441 aaagaataag actgttcata gacgtaaatt tctagaggag aaccttctaa taactcgaaa
     4501 gtaacttgtt catctgtgac tgagatgaac aataaacgtc cgcgataatt gatatggaaa
     4561 gaataagctt tccaagtttt tggcagaaat ggtgcgaatc tcaactgatc atgggcagtt
     4621 ttcatttgag caaatccttg gacaatcgac aaccagctac cagtcatgga agtgatgtgc
     4681 aagccgtctt ctgtgtcgtt attgtaatta tccaaatcca agcgagctgt tcgctggtac
     4741 atttctacag ctttgctttc cattcctaat tctgctgcta aaatcgaatg gatacttggc
     4801 gaaagggaag actcatggac agtcattggt tcatagaaat caaagttttt ttgcttttct
     4861 tcttttgtaa aagtatcatt gaagaaataa atcccctgta gtacatctgc ctgcttgatg
     4921 aagcaagatc taaggatttt gtcccaagac caatgctgat tcagaggaag atcgcttgtg
     4981 ggaatatcgg atacaggcat gagatcttta tctaagaatg tgtcatgctg gacaaagatt
     5041 cctaattctt cgtcaactgg gaagtacatg ttctctgcga tatctttcca tttggctagt
     5101 tcttcttcgc tgatagtgat agcagcttct ttttgataag ttaaataatt ttctcgcgtg
     5161 taacgcaaga cccaagctgc gatcgtattt gtataccaat tgttattgat attgttctca
     5221 tattcgtttg ggccggtcac accgtggatc atgtatttat ttgctcgttt tgaaaaatgg
     5281 acacggtctg cccagaagcg tgcgatttct gctaaaactt ccagtccttc atttttcaaa
     5341 tattccgtat cgccggtgta attcgtataa ttgtagattg cataggcaat cgcgccattt
     5401 cggtgaatct cctcgaatgt gatttcccac tcgttatggc attcgacacc agtgaaggtg
     5461 accataggat aaagcgcgcc ttttaatcct tgttgctgag cgttgtggat tgcttgcggc
     5521 agttgattat gccgatattt cagcagattc ttagttactt caggtttggc taaagcaaga
     5581 taaagaggga cagcatacgc ttctgtatcc cagtaagtgg ctccgccgta tttttctcct
     5641 gtgaatcctt tggggccgat attcaatcgt tcatcttcgc cgtagtaagt agagaatagt
     5701 tggaacaaat tgaatcgaat accttgttgt gcttcttcgt ctccttcgat gaccacatcg
     5761 gcaagcttcc atcgttctgc ccaagccgct gtttgctctt cttttgcttg gatataattg
     5821 tttgtctggt tcgttaattc tgagacagct ttttctgctt gctgcgcttc tggaatatct
     5881 ctactagtaa gaacgataat ttctttttcc actgaccatg tctcaccttg agataattct
     5941 gcagacaagc aaccatgtaa tgctaaagta tcttcgtgat agtcagggat gactgatttt
     6001 ccattaacga aatggcgcat tgctgcagta acagtaaatt gttcaatccc aaagttgttt
     6061 ggaatagttt tagtcgtcaa atagccgatt tcttctatag ttccacgatc aatctcattc
     6121 caaaaatgtt catcatagtt gctgtcttcg ttttgaacat ttccatccaa ttttgatacg
     6181 agtttgactg ttccgctacc ttcaagcatt tctatagtta aatagatata agctgattct
     6241 tttttgacaa tgctcagaaa acgttcgaag gaaatgcgta ctttattagt agaagtagtg
     6301 attgtgaatg tacgagataa tacaccattt ttcatatcta attcccagta aaagtcttct
     6361 ggttccaaag aagccaagtc gatcagctga tcgttgatcg aaatatctat cgcgataaag
     6421 ttcaaggcgt tgatcacttt tccaaagtat tctggataac catttttcca ccagccgaca
     6481 cgtgtcttat cgggatacca gacacccgcc aaataagtac cttgatggtg gtcccctgag
     6541 taagtttctt caaagttccc gcgcatcccc atataaccgt ttccaatact tgttaaagat
     6601 tcttgcagtc gaagattttt tttatctaat tgtgtcgtgc tgattttcca tggatcaata
     6661 gcaaatagtc gtttgatctg tttcattttt ctcctcctaa tgagctgttt tatacgttca
     6721 taataaacgc aaccgattgc acaagtcaat agacaaaaga aaatatttca tagaaaaaag
     6781 cttaaaaaaa taaattttta ggagaaaatt atagaaggga acacacgtat tataggtttt
     6841 atagaatggt atagacagaa aaataaaaaa taaagcattt acaaaaaaga aaacgcacgc
     6901 tataatacgt ttaacgaaac cgattgcgta aaggagctaa acggaatgaa aaagatattt
     6961 agttttgagt tttggcaaaa atttggaaag gctttaatgg ttgtggtagc agttatgccg
     7021 gcagctgggt tgatgatcag tatagggaaa tcaattccct tgatcaatcc tgattggacg
     7081 ccgcttgtca caacgggcgg agtaattgaa aatattggtt gggcaattat tggtaatctt
     7141 catattttat ttgctttagc aataggagga agttgggcaa aagaaagagc aggcggagca
     7201 tttgcagctg ggatctcatt tattttaatc aatcggatca ctggggctat ctttggtgtc
     7261 acaagtgaca tgcttgcgaa tgaagatgca tttacccaca ctctgttcgg tacgaagatc
     7321 atggttaaag gattttttac aagtgtattg gaagcaccag ctttgaatat gggagtattc
     7381 gtagggatca tcgctggatt tgtcggggca atggcttata ataagtacta taattataga
     7441 aaattgccgg atgctttgtc atttttcaat ggaaagcgct ttgttccgtt tgttgttatc
     7501 ttatggtcaa caatcgtggc attgattctt gctgtggtgt ggccaaacgt acaggctgga
     7561 atcaataatt ttggtttatg gattgcagaa tcacaagaaa gcgcaccgat tctagcaccg
     7621 tttttatttg gaacattaga acgtttacta ttgccatttg gattgcatca tatgctgacg
     7681 attcctatca actatacaca gcttggcgga tcgtatgaga ttttatcagg cgcacaggcg
     7741 gggacacaag tatttgggca agatccgtta tggttggctt gggccactga tttagtgaat
     7801 cttaaaggtg caggggatat gtcgcaatat gagtttgtgt tgcataactg gacgccggct
     7861 cgtttcaaag taggacaaat gattggttct tcaggtatct tgatgggaat ggcttttgcg
     7921 atgtatcgta atgtcgatcc tgataagaaa gcaagatata aatcaatgta tttttcagca
     7981 gcgttggctg tctttctaac aggtgtaaca gaaccattgg agttcatgtt tatgtttgct
     8041 gctgtgccat tatatgtcat ttattcagtg atccaagggg ctgcatttgc gatggcagat
     8101 atcttgccat tgcgggtcca ttcatttggg aatatcgaat tgctgactcg gacaccgtta
     8161 gctatcaaag cgggtctagg tggagatctt atcaacttcg tgttgtgtgt gatcgctttt
     8221 ggtgttgtta cgtatttctt agcaaatttc ttgatcaaaa aattcaactt tgctacaccg
     8281 ggacgtaacg gaaattacga caatgacagt gaagaaaccg ttgtatctaa cagcggaaca
     8341 ggaacagcag atcagcaagt ggtccagatc attcatttgc tgggaggaaa agaaaatatc
     8401 aaagatgtcg atgcttgtat gactcggtta cgcgtaagtg taaatgatcg agaaaaagta
     8461 gggactgaag aagcctggaa acgagcaggg gcgatgggat tgatcgtcaa agacaatgga
     8521 gtccaagcag tttatggacc aaaagcagat gtcttgaaat cagatatcga agatctgctg
     8581 caatcaggag cagagattcc agagccagaa atggtttcta caggaaatgg acaagcaggt
     8641 agtaagcaat acttaggtat agaacaagaa ctagtttccg cagcttctgg agaagtgatt
     8701 cctttatctg aagtaaaaga tccggtattc tcacaaaaaa tgatgggtga cggctttgct
     8761 gttattccaa catcaagaga agttgttgcg ccgatcgctg gaaaagtgac aagtattttc
     8821 cctagcaaac atgcaattgg gctggaaacg aaagatggta tcgaagtttt gatccatatg
     8881 ggaatcgata cagtccaaat gaatcagcca gcttttgaga ttgctgtcaa agaaggccaa
     8941 gaagtaggaa caggaacgaa attagctgag atggatcttg atgtgatcca aaacgaagaa
     9001 aaagatccaa cgatcatgat cgtatttaca aacgataaag tagaagaagt agtgatcaag
     9061 caattaggta cagtgacagc tggtaaagtg atcggctcca ttaaaatgta aggagaggtt
     9121 gtgaaaaagc tgtcctgcat caagtcataa gaacagccaa acaaaaatag ctcctcctat
     9181 ttttcgtttg gttgggctta ttaccgagaa gcagcagttt gaatactgtt tagtggagag
     9241 gtcgtgaaaa agctgccctg caccaagtga taaaaacagc caaacaaaaa atagctcctg
     9301 gtccattgag cttattactg aggagttagc aggagaacac tatttgctgg agaggttgtg
     9361 acaaaaattt tgtcacaacc cttttgtttc gaaatgaaga caaggagaaa accaatgaaa
     9421 ttcatgacac tcaacacgca tagctggctt gagccagaac cagagaaaaa attgcaagaa
     9481 ttagcagata agatcctgtt agaagattat gaagtgattg ctttacaaga aatcaaccaa
     9541 ttactggaat ctgaagaagt agaacctgcg aagctaatga agttctgtcc ggtaaagaat
     9601 caagtaccta tccgtaaaga taattttgcc taccgtttag ttcagctttt aaaggaacac
     9661 ggaaaagagt atttttggag ttgggaaatg agccacatcg gttatgataa atatgaagaa
     9721 ggaaatgcac tgcttacgaa aaaaacattg gaaagtcagg tccttaccgt ctcccaattt
     9781 caaaagaaag aagattatca gacacgcaag atcttgattg ggaaaacaaa gatagatgat
     9841 caggacattt ttgtctcgag ctgccacttt tcttggtgga cagataaaaa aagtggcttt
     9901 tattttgagt ggaaaaactt ggaaaactac tttttggaaa ctcgcgtacc actttttttc
     9961 ctaggtgatt tcaataatcc agtagactct caaggttatt atactgtaag agaaagttgc
    10021 cttcttcttc aagattcgta tgttgtcgcg aatgaaaaag gaaaagcagc tactgtggaa
    10081 aagaaaatag atggttggga acaaaataca gaaaaattgc gtatagattt tattttcgtc
    10141 cctgaaggaa tgcaggttaa aaaatatcaa cggatttttg atgggatcga tagtccaatc
    10201 atcagtgatc actacggggt ggaaatcgaa ttagatgtga acgaataaaa aatataacat
    10261 ataacataaa aaaactgatt aaaataaaac tctccaaacg attataccgt ttggagagtt
    10321 ttttgatcgg atgtatttat cccttttgcc taagcacgta aaagcttgta tctatgataa
    10381 ataaataaac atagcaaggt aaaccagcca atcaaagtaa gagaaagaat gaaatagaaa
    10441 tagattggcg tttgataatg aagcgtgagc tcgttgattc ctttttgctg cttcacgatt
    10501 ggggtcccga tatctgttac ttcataatca gtcaatgttt ttccattttg ctgtagcaca
    10561 gtccgatcgt agacgataac agggatatga acagttttgt ccccagtgtt tttccacttg
    10621 attgtcaaaa gaccgcctgg atgtgttttt ataaaagttg tttcgttttc tagtatttct
    10681 tcgttatacc ggtcgtattt attgtctttt gtctccttat agattggcag atagtcaggt
    10741 gtggtttttt ggaaaacgaa caagctggta gacatatctt tgctgaagaa tgttttcctt
    10801 gcttcttccg gcgtatcgaa tagataagta tgtcgactga caaatgtttc attttcccac
    10861 ttttccagat gctggtataa gttctggatc gtctgtgctg aaaaaataag aatgccaaat
    10921 atacttaaaa gacggaacca cttttgttca gaccactggt tcagtgaaag ggcaaggtaa
    10981 agtagaagca atagcgtaaa tggcacgaaa aatcgaaatg gaaactggat caaactaata
    11041 aaaggcacat ttttttcgat caatgtcgtc catggaatca agctagtaga aagaaaaaag
    11101 aaaacaagac ttgtgtacaa cacgattttt tggaataagg tgttcttttg ttgaaagaaa
    11161 ataaaaagca aaaaacctga taatatcaat ataggaaagg ccttagggta atggagccaa
    11221 taactgccat ccagatctat cgccttttga ctcatttctc tgttaacaaa gggaggaaga
    11281 atcttattcc cactgtaaac aatagctaat cctgcccaaa tatttgctgt cagaacaaaa
    11341 taaagcaaga ccgacttaat cagttgaagc agtgcttttc ctttttcagt ctggttaaaa
    11401 aagtaataaa gataaaaagg gatatacatc aaagctaaca tgatcgaaga cagtaaatgg
    11461 atctgtgtca taagtcccac tgagacagcg actttgataa cagggaactt tttatgaagg
    11521 acgaaatcag caatagggat catacataaa ggataaaaac aagtccccca gctagtgaat
    11581 ccttggttga tagaccagta ttgaatagaa tacgtactca tgaataaaac ggctactggt
    11641 acacttaccc aatttcgaac attgacatat ctcagtaaaa tcagcataga aaatcctgaa
    11701 ataagataaa gaatgaaatt tgaaagaatc tgataatgaa accagctacc tgcaagcaac
    11761 acgatcaatc cttgtaaata agcgaaaaat gggccgtaaa cggcattcac gatcctgcca
    11821 ctttgctgga atccataaag ggaaggaaaa taatggaaat ccccatattt aatttgttgc
    11881 gcggcatcat agaaccgatt gtaatggaat ggggcatcag cacccaaaat tagtttatgt
    11941 gtgaataaat gaggcatgag aaatgccaaa gcaatgaaaa aaattgctaa aaatgggcca
    12001 tattttttca attgtcgaac tcctttgcaa actttagata aattcatcat atatcctatg
    12061 cgagaggtag gcaagaaatt agaaaattca tgaaaatatt tatgaatttt acaagaaaaa
    12121 cataagtgaa agttctaagg agagaggatg cgacataagt tttgttgcat ctccttgttt
    12181 tgaaacagaa tttttcttaa tactcgaaac aaagtagctt tttcatgacc tctcggtaaa
    12241 cggtgttcaa tcagctgctt ctcggtaata agctcactag cctagaaaat atgagaagct
    12301 attttctgac tagcgctctt attactcaaa gcataacgca gttttcacaa cctcttcctt
    12361 aaacggtgtt caaaagcttg catctgcggt aataagccca acccaacaaa aaatatgagt
    12421 agctattttt ttgggctgta cttattactt gatgcaggac agctttttca caacctcatc
    12481 ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac aaaaaaactt
    12541 gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac ttttgtttcg
    12601 tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc cattttatgt
    12661 ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa aacggaccgc
    12721 agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat taactgacca
    12781 aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa gtacaacatc
    12841 cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc aattaaatat
    12901 ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg tcttgcaagg
    12961 aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc acagcggttt
    13021 acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca cgtttatttt
    13081 gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg tactgccaga
    13141 tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc ttacatgtac
    13201 gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc cttataacga
    13261 atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc aatggtttat
    13321 tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac gactaatcta
    13381 tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg attcgtcagg
    13441 gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac ttactagaaa
    13501 tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg atcaattgcc
    13561 tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt cttttggtat
    13621 tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg taaaagagct
    13681 tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa aaatgatccg
    13741 cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc
    13801 taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag tagaatctgt
    13861 taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag aaattattgg
    13921 ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg aataccggca
    13981 acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa cactgacttt
    14041 gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag gttttacaat
    14101 tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga aatggaatca
    14161 aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa ttttttgctg
    14221 ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag ttgttacaat
    14281 ctttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
    14341 tgaggaacta tttttccatt tgtttctcta atactcaggt caaaacgctg atttcacaac
    14401 ctcttgataa acggtgttca aacggctgct tctcggtaat aagcccacta gcctagaaaa
    14461 tatgaaaagc tattttctaa ctagcgttct tattactcaa agcataacgc tgatttcaca
    14521 acctcttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
    14581 acatgaggaa ctatttttcc gtttctttct ctaatactca ggtcagaacg ctgatttcac
    14641 aacctctctt tcacaacctt attttactcg tttataattt tcatcgaagt attttccgct
    14701 acggatatca tctggcattc catcgtatgg ttcttcgcta ataacatctt cattgtctat
    14761 acctgcatca cccatataag tgatcgtagc aaattcagga acaagtaatt ttggataagt
    14821 ttcatatttt tcacgagctg cttccatttc tgcgatatgt tcgttttgga aagtaacagt
    14881 gatttctgga tgttcttcaa cccatttagg gaaaaagatc attccgtgaa gtttcttttc
    14941 atttggcata aagtccaatg ccacgtctgt ccctgttggc tctgtccaag tgattttgaa
    15001 tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct tttacccaac ggcctttgac
    15061 cattccgcca tggatacggt aatcaacagt atggtcattt ttggcatacc attcatattc
    15121 ccaaccattg tcatacgtat agataaagtg agttccaata aaatcttcca attctttaaa
    15181 ttctttcatg tttaagcatc tccttataaa ttaaaacatg taataaacta catgttttcg
    15241 attactcatt tatgatagaa tggatttaaa gatttgtcaa ggagggaaaa ctgatggcaa
    15301 gaaaaaatac acttcgatat attttgttag ggttattaag taagaagaag atgacaggat
    15361 atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt ttggcaagca aaacatagtc
    15421 agatttatcc tgaacttgca aaaatggaag agcaaggaat cattcagcat caagtagaga
    15481 tcacaggtga aaaattggaa aagaaagttt atgaattaac agaagaaggg aaggaacaat
    15541 tggcagagtg gattcataca cccaccaatg aactaccggt taatcgagac gaatttgttc
    15601 tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt agtagaaatt attcagcagc
    15661 agcgtgacct tcatgaagaa aaacgacttc atctattaga gcgaaaaaaa acgatctttc
    15721 ctactgaaaa agaaaaacat gaaaattacg gacactacct gattttagcg catgcgatca
    15781 atcgggaaac agaatatgca aattggctaa cagatgtatt agaagaaagt aaccgaaaaa
    15841 aataaaaaaa tatccgaaca gtgatggaga attcctttct atatgaaaga aacgcagatc
    15901 accgttcgga tattcttatg ttcaaacagg ccttttttca agtatttatg tagcatctac
    15961 ttattaaaaa gcttggtact taggagatta gtggcgttta gagttctttt aacaactgtt
    16021 cgtagtcagc tttttcatcg acaggaagga gcttgtaagt taattcgtct gtctcgttgt
    16081 catgaatgat gtacatctta gcattctctt ctttgaagtt accgatttgt aaaatcgtat
    16141 agcgttcgtc gatcaattcg atatctttga ttgcttttga aaaaacttta cggacatggt
    16201 gcattttctt ttggcggatt tctgcctttt ctgcagcttt tgacaaagta aatccgtcat
    16261 ctaaatattt tttgatcagt tctactttta tgactgtagg taaacgatat tttctaggac
    16321 aattcgcatc agcttcgatc gaacgaatga atcccttttg ttcccaatac cttaattgac
    16381 gtggagacac acctacgata tcacttaatt cactgatgcc aacaaccaat tgatcatttt
    16441 ccaacaaacg tttaagggaa gccccaacca tttttgtcac tccttttgtc ctcttgcttc
    16501 ccttaattat atccgtatta acttatgtgt caagtaggtt gacaaatagc gaatttagga
    16561 ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa ggagaaatat gagtaacaac
    16621 caacccgtag atatttacgg aaaaccatat aatcggacac ttttagttgt tgtcttatta
    16681 attggtacat tttgtaccgt tttgaatcag accttattga cgacagcttt tcctacattg
    16741 atgaaagctt tcgatatttc agcgtctgat gtgcaatggt taacgactgg tttcttgcta
    16801 gtcaacggga tcatgatccc aataacagca tggctgatca ataaattcag ttcaagaaat
    16861 ttatatcttt ctgccatgac gatctttttg atcggaacga ttacttgctt tacagcacct
    16921 aattttagta cattactaat cggtcgtttg atccaagctt gcggtgtggg agtatcgatg
    16981 ccattattgc aaaatatcat gctatctatt ttcccgccag aaaaaagagg atcagcaatg
    17041 gggatgtcag ggatcgttat tggacttgct cctgctttag gccctacatt atctggttat
    17101 atcattgata actaccattg gcgtgacttg tttggtatgg tactgccaat cgttgttctt
    17161 gtcttggttt tagcattctt cctaatgaaa agtgttattc cattgtcaaa tcctagcatt
    17221 gatattttat ccgctatttt atcaacgatt ggattcggta gtttacttta cggattttct
    17281 agtgtaggag atgacggctg gggaagcttg aaagtaattg gtttcttagt tgttggtgtg
    17341 attttcatcg ccttgttctc atggcgtcaa ttacatttgg aacatccatt cttggaactt
    17401 cgtgtattca aatcatctac attcacgatt gcagctgttt tagctggtgt aacaaatatg
    17461 gcgatggtag gggcagaaat ggtcgtacca ttgtatattc aaaatattcg tggagaatca
    17521 gcattccatt ctggcttgat gttgttgccg ggtgcattga tcatgggggc tatgatgcct
    17581 gtgacgggtg cgattttcga taaatacggc gcgaaacgtt tagccatcac agggatgttc
    17641 attttgacag cggcaactgc accatttgct ttcttgacaa aaacgactcc agttttatat
    17701 atcgtgatcc tttatgcgat ccgtatgttt gggatctcca tggtcatgat gccggtaacg
    17761 acttctggta tgaatgcatt gccaaataac ttgatcagtc atgggacagc ggtaaataat
    17821 acattccgtc aaatcgcaag ctcgattggt acagctattt tgatcagtgt gttgacgaat
    17881 gtgacaaaag caaatatccc aagcaaatct gtattggata atacgccatt agcttataaa
    17941 gatcaggcaa tcaatgccac actgtcaggt taccatgccg cgtttatcgt agcgatcatc
    18001 tttgggattc ttggctttat tattgccttt ttattgaaga aaaaagaacc tgttctaatg
    18061 aaagcggggg ataatgcatg attattggag ttttgattgt cagcttgctt gcttttgctt
    18121 taacgaacgt gttcgctaaa aaaacatggc aaaccttttt atctttgatt ttcggactaa
    18181 tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc caatcacttt ggaatggaaa
    18241 aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc tgattcccaa ggagcagata
    18301 tgcttttgta taaagcttta gggaatggaa aagaaaaagt gtatctttac cgaacaaatg
    18361 aaaaacaaga aaaaccaaaa gcaactggta cggataacga aacaaataaa gtagaaaaga
    18421 cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg ggaatacaaa aacgacatgt
    18481 ataaattctg gttcaatatt gctgataaca atcacgaata tgacagccga gtgaatacat
    18541 tcaaaatccc agagacatgg gtagaattaa gtacggacca agcagccaag ttagctgaat
    18601 tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa agcttatgtt caagacggta
    18661 tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc agagcaagaa caacgtacga
    18721 aagccttagc tgctgaattc caacaacaag cattcgcaaa attagtgaaa gaagcaaaag
    18781 gcgaataata ttttgaacac catgtaggag gttgtgaaaa agctgctctg catcaagcag
    18841 taagaacaat cagataaaaa tagcactcca tatttttcat ctgattgggc ttagtgccgt
    18901 agatgcaagc ttttgaacac cgtttaagga agaggttgtg agcctgccgt ttaactctga
    18961 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg ttgactattg tgacttgtga
    19021 ccgaggagtt ggcaggagaa caccgtttat tcgaaaaaag gaactgcgac aagacttttg
    19081 tcacagttcc ttcttttttt tgctatgcta tactagatgt aaaaaatagg agaaataaaa
    19141 tgagggaaat ttattctttt gaatcaaaaa tctatgcttc cgaagtcggc aagggcgggg
    19201 catatattat ttttccgtat gatataagaa aagaattcgg tgctggaaga gttaaagtcc
    19261 aagcaacttt tgacaatgtg ccgtatgaag gcagtatcgt gaatatgggc gtgaaaaatc
    19321 cagatggttc agtctgctac atccttggac tacgaaaaga tattcgtaaa aacatcggaa
    19381 aagaaatcgg tgatattgta gcagttacag tgaagcaaaa ataaaagagg ttgtgacaaa
    19441 agacgtgtca caacctcttg ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca
    19501 aatttatgca aaaatgtgga aacccatgtt tacaaaattt ttcttactac tcgaaacgaa
    19561 gcggctttat cacaacctct tgataaacgg cgttcaatca gctgtccctc ggtattagct
    19621 caaaaacggc aaaatatgag aagctatttt tccatttttt tctctcatac tcaggtcata
    19681 acgctgattt cacaacctct tctttcataa tggtttttca ccatctttat aataaactgg
    19741 ttatttgata tggaaatcta ttttttccaa ccaatctaaa atcttgtaac ctaactcttc
    19801 tgacatcgga aaagaggcgt tgatttgtgc attggagata tggtcaacat ggataccgga
    19861 agtgatcgta caacttcccg gaacggcagt tttgatgatc acagctattt tttcggctaa
    19921 gacatcgtct ttatggaacc gaccatcgtg gctagggaaa cgtacagttt gcatctctgt
    19981 atcacgagaa aatgtagtga ctgttccgat atgtggtgta tctccgcctg ttaaagtgat
    20041 caataagtct ttaccgatta ctttaacagc agcttggatc gtgaaaccta gtttggttac
    20101 ttcaactatc gtttcttttt ccatattaat caccctgcca acgttttgaa tgctcattta
    20161 caatttcaaa ttgatccaaa agtttcattg tatggtgatt aacaagatct tggatcgttt
    20221 gaggctgatt ataaaaagca ggaattggtg gtatgatttg cacacctagt cgtgctaatt
    20281 tagtcaagtt ttccaagtga atggcattta atggcgtttc acgaggaaca atgactaact
    20341 ttcgttgctc cttcattgtt acatcagcag cacgtgcaat caggttgtcg cctaaaccaa
    20401 cagagatacc tgcgactgtt ttcatacttg ccggtacgat gatcattcca tcggtcaaaa
    20461 atgatccgct ggcgacagca gcacctaaat ctttgtttga ataatggtaa tcaaacaggc
    20521 tttcgaattc agttagtgat aagtcggatt ccagctttaa gttctgtttt gcccatggac
    20581 tgatgattcc gtgtgtttct atcatcggat attcttttag tttcttcact aggttgatgg
    20641 cataaatggt gcctgaagcg ccacttacac caatcacgat ttttttcttc cgttgtgcca
    20701 attgttccat tttccgcact cctttttatg atgtcaaaac agttgcagat cattcattat
    20761 ttcaaatatt tttcccaatc ttctacttct tggaattttg cacgtacaaa atgttctttc
    20821 atatcgaaag gaactgtacc gtcaaagata gtttttgcac ttatcccacg gaaacgaata
    20881 gagccaggat tatattctgg taattctgat gggtccaatg gatggttcct cattcctggg
    20941 atcacgatga tatctttatc tccttggaaa cgagtgttga aagtccaaac tacatcgttc
    21001 atgtcgaaaa tatcaacatc ttcatcgact aaaatgactg tcttcaattc tttgaaggct
    21061 gataaagcaa gaatagctgc ttgacgctga ataccttcat ccgcttcgtt ttctttacgg
    21121 atttgcatga tggtcatcaa tttaccacca cctgcaggag gattgtagac atttactact
    21181 tttcctggaa tagctttatc gaccaaggca agaatacttg cttctgtagg aataccggcc
    21241 atagatacgt gttcttcact gggaccaatc gttgtttgca tgattggatt gtcttttcgg
    21301 tgagtaactg ctttgacttt cactacttgt acagctggat tagcatcccc agtatagcca
    21361 ggaaattctg gcattgcttt tcctgagttc gtgttgatat cttcttgcat tgtttcattt
    21421 ggcatgattt ctgcttcaat gatgaattca gcacgagcaa tacccacttc atcgactgct
    21481 ataccatcaa cgatttgaat aggttcgtta cgcaaagctc ctgcacacca tagttcattg
    21541 tagccaagtg gagtggtagg tggttcgaaa gtcgtgccaa tggcaattgc tggatctaaa
    21601 ccaatattga tagtgatagg cattggttta ttcaaggctt cgaattgttt ttggaagtga
    21661 ccaatgtggc gtccgccagg catgatgtac ataccgatcg tatctttatc ttctagaacc
    21721 atacgatgga tcgttacatc gctcattgtt ttttctggat ctgatcctaa gactacaccc
    21781 attgtgatgt atggaccagc atcttcagga gtatttgtag gtgcaggcaa aatgttgcga
    21841 atatcgaagt ctgcatcggt agctagatga acgacttctt gtgctggagc gtctgctttg
    21901 tctactttca caggtttgat tggattttct acagcgtctt tcaagaaacg accaagtgtt
    21961 cgataatcat ggtgcaaaat ttcacctaca cgtttacgac tagccattgt tccgatgttc
    22021 acacgtgtgc cggggaaacc tttgatattg ttgaaagtca tagctggtcc ttcttgagtc
    22081 ggacgtttta ctgtaccgcc agcaccgata taacggtaaa caccagataa ttctgcttca
    22141 ggatcgattt caatatctgt ttcgtgatat tgacctggaa gtgtttttag ttcttcgatg
    22201 acttttctta aatcatacgg ttgatctgtc atgagagatt cctccttagt tgtctttacc
    22261 tatctatagt aaaaggtgaa aaagaggaaa acaattcaac ttgttcggta aagtattcta
    22321 caatggaatg gatgcgtttt tttgataata gaataaacta tgatatactt atcactaaaa
    22381 gaaagcgcat acttagtgtt ggaggaagga aagtgaaaaa aatggaggag tcatttccta
    22441 aagcagtaaa agtggaaaat atagcgaata ttttgaaagt tacatttgaa aacggagaag
    22501 taaaatacgt gaaatctcat tggacagaag agataaccga tgccttgcaa tttggaaaaa
    22561 aaggaagagg aaaaagaaaa aatcttctcg cactttctcg taatatgtgg attgggactg
    22621 aagtaacgat tgaagcagat ggaacagtat tcataaatgg aaaagacagg tatacgccag
    22681 aagagttgtg gtacaaagga aagaaaagta taccagaatt ataaaaaata attaaaaaaa
    22741 acagcgcgaa acaaggaaca tattacttcc tcgtttcttg ctgttttcta ctatttcagc
    22801 gctcttatga ctcaaatcta aatgtggttg tcacaacctt attctgcacg caaagcgata
    22861 gcggcgtctt ttttcgctgc cattcgtgca ggaagatgac cgccaagcat cgtcaatata
    22921 gtagagacga tcactagaat aatcgcatgg acaggattta gttgagcaac attttttaga
    22981 tccgtcatgt tgtataagat gctattgata gggaacgtag ctagccatgc aataaacaca
    23041 cctaatatcc cagaagaaat acctaaaata cacgtttctg cgtcaaatac gcgagtgatg
    23101 tcttttttac gtgcacctaa agcttttagt acaccgattt cttttgtccg ttcaatcaca
    23161 gatgtataag taatgatact gatcatgatc atacttgtta ctaaagagat ccctgcaaaa
    23221 gcaataagga cataagtgat agcatccatc aatccgccag tcagttcagt catcgttcca
    23281 gctaaatcag tataaatgat tttatcttca tctgatttgc ctttattgta atcatctaga
    23341 taatccaaga tcttttcttt gtcttcaaaa ttgttcggat aaatcatgat acttgaaggc
    23401 aagctgtctc cacctaaata actaagcaat gtttgtttgg cattttcgtc aactttttca
    23461 gtagttaaga catttacacc actgtctctt tgagctttga cgatttctga ttctttgttt
    23521 tcgttgacga tttgagtcgt taattgatcg ctataagcga ttcctggaga taagagattc
    23581 attgtagaag aagatttcac gcgtaggatc cccgagatct tcaattcatc agaagcattc
    23641 tgatacatcg catcataatc tgtattaggg ataaaattcc cagttggaag ttttgtatag
    23701 taagtattgt tgtttactaa tttgaatgtt gtaccaacga tatcatcaaa atcaagtttt
    23761 tcgtcttctt tcacatcaaa tccaagattc ttcaatgcgt tgatattagt attattattg
    23821 ccgtctacga tcagcacgac atctgttgct gaggctggat aacttccggc aagtaaagaa
    23881 taattgtctt ttaaaaagtt ttctttgcta gtgtcaagct gtgtagggaa acttgacact
    23941 cccacacctg tcattgctga catcgtacta gataaagaaa gagattccgt atcaggattt
    24001 tgattcgaga agctgacagg ctgtactttg ccatttacat cacgtaaaag attgattcct
    24061 gtagtacggg taaatccgat attattactt aagttaggat cgatgtctgt tacgtaatcg
    24121 acgtatgttt gatcaatctt attcgtgtgc tgtgctttat cttcttcgct gacttttgca
    24181 gtaatcgttt tgctgtctgg ataatccgct ttgcttgcac ccaatccggc atcgtcacga
    24241 gtctggttag tcgttacctt agaaatagta atcggaaatt tcgccatcgt ttcagcttgc
    24301 gtgttatcga tttgtttttg gaatccagaa gaaagggaca gaacgatcgc gattccgatg
    24361 atcccaatac tggaagcaaa ggaggtcaag aaagtacgtc ctttttttgt tcggatattg
    24421 ttgaaggaaa gctttaacgc tgtccaaaaa ctcatctttg ttctgcgtaa gttaaattga
    24481 tcatcttttg gtcgttcgat gtgaggatgg gaatctgtta agatcttacc atcagaaaat
    24541 tcaatgattc gatctgcgta ttgatgggcc agttcaggat tatgtgtgac catgatcact
    24601 aatttttctt tcgataactc ttggatcaat tccatgatct ggatacttgt ttctgtatct
    24661 aacgctccgg ttggttcatc gcacaaaagg atatctgggt cattggctag tgctcgagcg
    24721 atggccactc gctgcatttg tccaccagat aactgatttg gctttttatg catatgattt
    24781 gtcaagccga ctcggtgcaa tgcatcttct gcccgttttc gtttttcgtc tttagaaaca
    24841 ccacttaatg tcatcccaag ttcaacgttt tcaataatgc ctagatgacc aatcaagttg
    24901 taactttgaa aaatgaaacc gatagagttg tttcggtagg catcccagtc actgtcctta
    24961 aagtctttag tagactttcc gttgattacc atatcaccgg aatcataatt atctagtcca
    25021 ccaatcacat tcagcatagt cgttttacca gaaccactag gacctaaaat cgctacgaat
    25081 tctttttgtc tgaaagcgac agacacacca tccaatgctt tggtggtggt ttcgccgact
    25141 ttataatatt tttttatatc ttttaattga agcataatca cccttctccc gtattgaaat
    25201 attcggtata ctaataagta tatcagaaca atgatcgtag tatacgaaat aaatgttaac
    25261 tgaagatgaa ctacaagaaa aaatctagaa atttttcaaa aaacagaggg agatcaatga
    25321 atcgtatttt agtagtagaa gatgatccgc aattacagtt gctttaccgt tctgtgttgg
    25381 aacgagcggg cttttcagtc atcaccgcat tgaatggaga ggatgcgctg aaaaaactag
    25441 aagcgacacg tatagaatta atcattacag atatcatgat gccgaaaatg gatggctatg
    25501 acttgctaga ctcattacgt agcaccagat ttgacacacc tgttctaatg attacggcaa
    25561 aggccgattt tgaggataag aaacgcgggt tcaaattagg agcagatgat tatatgacaa
    25621 aacccatcga tgtcaatgag atgatcctac gtgtggaagc attgcttcga cgttcaaaga
    25681 tcaattacag cagtagcttg ataatcggac aaacaaaatt agagctagat gcttatacag
    25741 tgttacaaga tcagcattcg ttgattctgc ctcaaaaaga atttctgctt ctgtataaat
    25801 tactgtctta tcccaacaag atcttcactc gccaacagtt gatggatgat atttggggat
    25861 tagatacgaa tacagaagaa cgaacgatcg atgtacatat caaacgttta aggacccgtt
    25921 ttgaaaagac ggaagatttt caaatcatca cagtacgggg actaggctat aaggcggtta
    25981 tttaaagatg aaaaaaaagc tattttcaca attatggatc tattttgcta gtattgtctt
    26041 catttcgatc ttagtgacac tgctgtgttt tctcggtttt attttcttat tgtctcgaca
    26101 ttctatctcg cctgctgaat cggctggaaa accaacgtta tacccgttat tcgtctttgc
    26161 ggggttcagt atggtcgtgg gtacaggaat atcgattttt gttgggcggc gtatcctgca
    26221 tccaatcagt gcgctaggga caaatatgag tctggtagca acgggagatt tttcgattcg
    26281 aatggatgaa cagcagaaag tagcagaagt acagcaactg tataaagatt tcaacgttat
    26341 ggtccaagaa ctcaatagta tcgagacact gaggaatgac tttgtgtcca gtgtatccca
    26401 tgaattcaaa acaccactag caacaatcca aggatatgtt caattgttac aagccccaaa
    26461 tctctctgac gaagaacgcc aaatattttt atatcggatt atcgaaagta tcacgcagct
    26521 ttcacaatta acagagaaca tcttaaaatt gaacaaactg gaaaaccagc gtatccaact
    26581 ggaaaagaaa gagtatcgat tagatgaaca aatcagagaa gtaatcgttt tttttcagcc
    26641 taaatgggag aaagaacagc tagaattgga tatcgaactt gctgcagtaa attacacagg
    26701 caatgaagag tttttgtacc aagtgtggct gaatatcatg gataacgcca taaagtacaa
    26761 tcaaatcaat ggacagattc atatcaaact atttgagaca gccacagaga ttgtactgga
    26821 agtgaccgat tcaggtgtag gaatgaatga agagaccaga gatcgaatgt ttgaaaaatt
    26881 ttatcaagga gatacaagtc gtcaaatttc tgggaatggg ctaggacttt cactggttaa
    26941 aaaaattcta gaactgcatg atggacgtat tgattatagc agtatcgaag gagtaggaac
    27001 gacagcaatg atacggttaa gtaaacaata ggaaaaagaa taaaacccct aaaaattcta
    27061 ttgaaggttt ttaggggttt ttagtcgttt ttttgtgatt agggaaatgt tcttacggtt
    27121 cttttggcat aaaaatcgct gctttcttat tgaactacga gaaatgttag aggtgtcatt
    27181 gaaacaagga catgatgcaa gaaagagagc gaaagtcatg cgtcgctctc tttttttggg
    27241 tggatagatg gacagaaaat gtagggctta gttttcttct tgatttaaat actcccaaaa
    27301 tgtatctgaa gccaaagaat gctcaccgac tttacgagta aatgcaagat aactctctgt
    27361 tgttggagaa atagggagaa tcacagtatt ttcttttaat agattcttga ctgttttttc
    27421 cataacaata gcaatcccca tgtcatttgc aatcatattg atgatcaagt cgattcttgc
    27481 tcctttataa gagatttttg gttcaaatcc agcttcgcga cacagatcta gtacaggttg
    27541 atacaagttc gtagtggggc caaggagcaa aaagttctcc tttttcagtt cgcttagatc
    27601 caatttcttt ttactagcta aaggatgatt tttaggtaga actgctacaa aatcgtctgt
    27661 ttcagtaggc aaccactcaa tcgaacttgg tggggcctgg aaacttctag caaagtggat
    27721 cgtgtcacta tgttcattgg cgtcattgta agttaattcg acactttcca cttctttcaa
    27781 ctggactgta aattcaggat ggcgtttcaa aaactcggtg attttggaaa aacctttata
    27841 attgatcatt gtcggtatcg ttaagatatt taatgaaaga ttgttgtctt tcgtataggt
    27901 ttcaagcact tcttgcatag catggtattg ttctacgaca ttttttacat atgggagcaa
    27961 taagcttcca gcttcggtca attcgatttt tctatgggcg cgttcgaata atttgacgtc
    28021 taattctgtt tctaacgaaa gaatctgttt agaaatattt ccttgggtgg tgaattgtcg
    28081 atcagcagtt ttggtgaaat tcattgtttc ggctaaatca agaaaaactt cgaatttatg
    28141 gatatccatc atattcctcc tacttattcc aataaagaat actttaacac aaaaaatgaa
    28201 ttggtgtaat cttctctgtc tgttacaatc tttcttgcag cgaaagcaat aattacagaa
    28261 atttttctga tttttttcgc ttcgtagaaa aagagaggag ttttttgatg ccaactggag
    28321 ttttgatcaa tgtaggttcc gttttgtttg gcggactaat tggcggatta gtaggaaata
    28381 aattaagtga acattttaaa gcacagctga cgatggtttt tggtgtgtgt tcgatgggga
    28441 tgggaatcta ttccattgcg ccgatgaaaa atatgccggc tgttattttt gctttagtga
    28501 taggaacagc gattggtttg attgttcatt taggtaatgg gataaataag ggggctgcgc
    28561 tgatgcaagt acctatttct aaaatcttcc ctagtgaaaa gcttggtatg acccacgatg
    28621 aatttatcag cacattagtg acggtcattg ttctattttg tgcaagtggt acggggatct
    28681 atggaagttt agattctggg atgacaggag acagtacgat tttgatttct aaatctgtcc
    28741 ttgatttttt cacagcagct atttttgcgt gtaatttagg gtatgtcgtt tctgtgatag
    28801 caattcccca attcattatt ttttaaattt tgttcttatt ggcaaaattt atttatccat
    28861 tgactacacc agacatgatc ttagatttta aagcttgcgg cggattcttg atggtcgcta
    28921 ccggttttag aatgatcaac gtgaaaatgt tcccagtagc tgacatgata cctgcaatga
    28981 tcgttatcat gccactgagc tggatgtgga caaattggat tatgccactg ttataataaa
    29041 aatcaaagag aagccatttt ttgctgacta aaaaggatca aatcttttag tcagtttttt
    29101 tgtatttgac aaactgattt tgaaactata ttatatgttg tataatatag attgaaataa
    29161 aatattacac ttgagaatag aaggtgacgt aatgcacatc cagattccaa ctgttcttct
    29221 tgatggtact gtgctagcag cattaagcaa ggaagatatg tatgggtatg ccttaacaaa
    29281 aatcgtacaa gaaagtctct ctgtcagtga atctacacta tatccggttt tgagaagatt
    29341 gaaaaaagaa ggatatcttg agacctatga tgaacctttt gaaggcagaa accgtcgata
    29401 ttactcgttg acaccaatgg gaagtcgtca tttagaagaa attattgatg aatggattgt
    29461 ttttcgagat tctttagacc ggttattgga ggagagcaca aatgaatgaa tatttgaatg
    29521 aagttgtgga tcatcttcaa gaaattcctg aagacgatcg ttttgatttg atccagtatt
    29581 atgaggaata ttttttagat tctggaaaaa cattagatca gatcattgat gaatatggta
    29641 caccaaagca gtttgcctta aaattaaaaa tcagttactt ttcagaagaa gaacaagcag
    29701 aaaacgagtt agtgaaaaat ccaaaaagac aaattcgcct gatttggtta atcattatcg
    29761 gtttatttgc ttctcctgtc ctcattccac tagcactggg gtttatattg gttattgggg
    29821 gactattggt tgctttacta gcgctcattt ttagtatcta tgttttatgt atcagtatct
    29881 taggtgttgg cctattcatg atcatctcag gaatcagtgt gtttggacaa tcgattctga
    29941 gtggattatt ctttatcggt gtcggtctgt tcgccacagg aagcgcagta tttatttcgc
    30001 cactgttgct gaaaggaaca aaagggcttt tttatgtcat gatgaacttt gttaaatgga
    30061 tgggacgacg ttttattaca aaaagagatc tgcattcagc gggcgtttga aggaggacac
    30121 ataaattgag attgaaacat tttttgatta ccggtattgg tctgatgctt actggtggcc
    30181 tcctagcagg tgcaacttat acattaggcg ctcaaaagag tctgacttgg gataatggtc
    30241 ctcggttgat cgatatggtt actgagacaa aaaaaatcaa ggatgcaaac aaaatcgtca
    30301 tccatgcaga aaatcaaaca gtttcggtca tacgcggatt ggattttgag ataaaaacga
    30361 cttatgataa gacagaaaag ccagaaataa aggagaaaaa tggcgtgttg actgtgaatg
    30421 cagtcaaaaa atcaacaggt gcaatcatta gtttagaaga cacaagtgca caaatcacta
    30481 ttgttgtccc tgcagatcat gaattatctg aattgacaat cgaagggaat aatagtgcaa
    30541 ttgatatcaa cgaagtatca atcaaaaaag tgaatactaa gacacatgat ggctgggtat
    30601 cgttcaatga atttacagga ggaacaataa ctgcgaaaga tgaattaggc tcacttcgat
    30661 tagatgaggc caatgtggat aacttgatcg tcacaggtga aaacagcaac ttccatctag
    30721 aagacagttt gatcagcaaa gaaggaaaag tcatcctaaa cggtggcaat ttatctttgc
    30781 atgagatgga aaacagtggc tatactatcc aaacgtctgg tgaatcttat attgaaaaag
    30841 actaccagcc aataacaaat actttgttag aacaaggaca gaaaaagcta aagatcactg
    30901 ctaaagacac atcgatcaat attacgacgg atcaagagga caatgaaaca gattgatcct
    30961 gtactcaact ggatatttta agaaaaaaca gcaaagatcc ttccaatctt tgctgttttt
    31021 catcgcttaa cgcacataac tatagtaaaa taagaaagaa aatatctaaa aagaaaacag
    31081 ggtgaaagag tatatgaagg tagaagaagg tcaaagagaa aagttaaaaa cagaaatgga
    31141 aagattacat acttatatca ctcaactaag ccaaaccttc tatgatccag acaaagaaaa
    31201 agtgatggtt aattacccga acaattcaga aggtcgccaa ttagagcaag tgtaccatga
    31261 agttttcaaa catttattga cggtgaagaa agagctggat tattattctt taccgattat
    31321 cgatacagga atattgaagt atgatgggaa gaaggaacga tttatcttta aatctgttcg
    31381 ggaagatttg cctctttcag cagggatgga tctagaggtc ttggtagaag actattttac
    31441 agaagaaaag cactgggttc gaacaaaatt ggattattta ccgcaggcag ccggagggac
    31501 acaggcaagc ggttggtaca tcactgaaga caaagagtta gaactagaag gtgtaatggc
    31561 gcgtattcga aaaaaaaatt aaaaaagtaa cattagatat ttattgaagg ataaaggagc
    31621 gcaggaagta gcatgagaga aaaaagagca ttttatatca atggttatgc agggattgtc
    31681 ggattggtcg tattagcttt agttggattg tttttcttct atttaggtat gtggcaagcg
    31741 aaagtttggg cacttttttt aagtattttt ctttggttga tcacgttatt gttgttaagt
    31801 tcggcaactg tagtcagtcc taatcaagca aaagtaattt tgttttttgg acaatattta
    31861 ggtacgattc gagaaaatgg ctttttcctt acgatacctt tggcacaaaa aatgactgtt
    31921 tcgttgaaag ttcggaattt caatagttct gttttaaaag tcaatgattt ggatggaaac
    31981 cccatcgaaa tctcagcagt agtagtcttt aaagtcgttg atacagcaaa ggcattattc
    32041 gatgtagcgt attatcaaga ttttgtagaa atccaaagtg agacagccat tcggcatatc
    32101 gctagccaat acccgtatga tacattcaat gagaatgatt tgacgcttcg aggaaacacg
    32161 acggcagtgt ctgacgagtt gcaaaaagag ctgcaagagc gtttagcagt agcaggtgtc
    32221 gaagtaatcg aaacacgctt gaaccattta gcctatgcaa cagaaatcgc tagtgccatg
    32281 ctccaacgtc aacaagcaaa agccattttg tctgctcgac aaaccattgt tgaaggagct
    32341 gtcacaatca ctcaaatggc cttagagcaa atcgaagatg gacaagaaat caatttcacc
    32401 gatgatcgga aagtccaatt gatcaataat ttacttgtct caatcattac agacaaagga
    32461 acgcaacctg ttatcaatac aggcgacatg aaagaccgta agtgacatac tttttgtaag
    32521 ataggaagaa ggagtttagg acggattatt gtcttgaact ctttttttag ttgttttaaa
    32581 gaaaatgaca aaattgaaag aagaatgata gttgattcga aggcagaatt gttcgctttt
    32641 ttctataatc agtatataaa gtaagagaga aggggaaaaa gtgaaaaaac ttctgatcgg
    32701 tctacttgtt ttcatcgttg tggtacttgc aggggtgaaa atcgcagatt acgtagttat
    32761 gggtggagaa gaatactatg tacaaattac gacagatggt acaaaaacag tttcaaaatc
    32821 agactctgga caagaaacag tcaactatag atatatcctg cctggctata cgaaagaggg
    32881 taaggaaaaa gaactggact tcaagggaca attgactcga ccgcttcgta aaggagcata
    32941 tttaaaaatc acctggaaca aaaataaagg tgtcacttcg tatgaagaag tcgaaaaaaa
    33001 agacgtgccg aaaacagcac tggaaaaact aattaaaggg tagggaaaga aatgaacaaa
    33061 atagtcgaag tacagaatgt aacaaaagta tacggaaaaa acaatgagaa aaagacgcaa
    33121 gcattaagcg gcatcagctt cgatgtagaa aaaggcgaat ttattgggat catgggcgct
    33181 tctggttctg gtaagtcgac acttttgaat attttatcca cattagataa accaacagat
    33241 gggcatatcc gaatcaatca aaaagatgtg accacattaa aaggaaatca actagcagat
    33301 tttcgtgcga atgagattgg ctttattttc caagatttca atttattaga aagcttgaca
    33361 gcacaagaaa atatcgctgt tcctctttct ttacaaggcg tcagaccaaa agaaatcaag
    33421 caacgtgtcc aaaaaattgc agaacgttta tccatctctc atattttgga aagctatcct
    33481 tctgagattt ccggtggaca aaaacaacgg gtcgccgccg cacgtgcatt gatcacacaa
    33541 ccaacgattc tattagggga tgagcctaca ggtgcgctgg attccaaaag tgcccgggat
    33601 cttttagata cgatggacga attgaataca aaagatcatg tatcgattct tcttgtgacg
    33661 catgatccgt tttctgcaag ttattgccaa cgtattttat tcatcaagga tggcggtatc
    33721 catcaagaag tgaaacgtgg agatcaaagc cgtgattcct tctatcgaga aatactatct
    33781 attcttggaa acttggaaca gtagggggag attgactatg ctttggaaat tatcacttac
    33841 tgggatcaag agccgtttaa gagactactt ggtgttattc tcaggattag ttatggcatc
    33901 tgctattttt tacatgtttg aatcaatggc aagcaacgaa gcttttttga aaagtaatac
    33961 gatcgttgcg gctacagtta tcattttccg attcggttct gttttactag ggatcatcac
    34021 attcgtttat attttatatg caaattcctt tttgatgacg atgcggcaaa aagactatgc
    34081 aatgtttatg atgctgggag caaaaggcag aaaaatcgca cagatgattt tttctgaaac
    34141 attcttagta gggatcgcag ctactttagt gggttcagct gtcggtgtag gattgacaag
    34201 tatcgtccac cgattattag tggatcagtt agatctcaat atttcgcatt ttacaccttt
    34261 tagtattcac gggttgttga tcactgtttt gttttttgct gttttgtttt tattagctgc
    34321 tgttgtcaat gctttttcga ttgtcaaaaa accgatttta gcattgattc gtgcggatca
    34381 gacaccaaca agaatcaagc agcataaatt cttgtttttc cttgaagtag tactgggaat
    34441 tgcttttcta ggctgcggtt actatatgat gaaacacgtg gggacctatc aagtctttgg
    34501 tataggggta gccttagtta cgatcgtatt aggaacttat ttcattttcc attctgtgat
    34561 cattttcttt ttatcattac taaagaaatc agaacagatt tcattgaaaa aattgaacaa
    34621 tttcacattg tcacagttaa actttcgtat tcgtgaatat acacaaatgc tctcaatggt
    34681 cgctatgctg tttgccttgg cactgggcgc tttgacggta ggattaggtt ttaaaaatga
    34741 aattgcagac ttgacagaaa acaattctgg atatgatcta gtgatgaata atcctcgggc
    34801 agaagatcag caaaaaatca aagaactttc accaacactt tctgcttctt atactcaaaa
    34861 agaagatgcg ggaacgattt actataatgc aagtgaattt gcggctcaac cattgatcat
    34921 gataaaacat gaagatggaa caccgccgaa gatcacgaaa gaaaaagttc ctgctaaaaa
    34981 aatgaatgaa ctgactgtac aagaggaact tcgaagctat gaattgccgg aacagcaaga
    35041 aaaagaaatc aaggtagtca gtcaatcaga atttgatcaa ctgaacttgc cacaatccac
    35101 tttacagcta gttcaggtaa aagacttcca agcaaacctg aaaccaatcg aaacacttgt
    35161 tgctcaaaat aaaatgaaca atccatcttt gcaaaaagta gagtacagca accagaaata
    35221 tgaaatgtat gaagtgtata atggtctatt ctctggtttt gaatttatgg gattcttcct
    35281 aggtttagct ttcttgacca tgcttgctag ctgtttgatg ttcaagatct tgtcaggaag
    35341 caagagtgac attgctcgtt atgaaatgct tgaaaagata ggaacacgac gcgggttgct
    35401 taaacaatcg atccgtagag aagtaggcgt gttattctta gcaccaggaa tcttaggtgt
    35461 agtccatgtc ttattcggat tacaaatgtt tggtctattc atgcaaaatc catacaaaga
    35521 tatttgggtt ccgtttacga tattcttcgt attgtatttc atctactatg tactgacgac
    35581 atggttatat actggaatcg tattgaaaaa gagattgtga aaaagctgtc ctgcatcaag
    35641 taataagaac agccaagcaa aaatagctcc tcatattttt cgtttggttg ggcttattac
    35701 cgcagatgca agcttttgaa caccgtttgt tcggaaaaag aggttgtgac aatatttttg
    35761 tcacaacctt tttttcacgg tcttttatta gaatatagta aattttttct cgttactcac
    35821 ggaataagct gttaagaaaa cggtttccat gtatactgga atcagtgcaa atgaaagaaa
    35881 agagggtgca aggaacatgg aaatcaatga acaaaatttt ggcgaaaact atcacttgat
    35941 tacgatcacg aatcaaaatg ggacatcttt gtctgtcagt gatcttgggg cacgggttgt
    36001 tcattttcaa acaataatcg ataatgaaaa acgagagttg atcttaggtt ttgatgaagc
    36061 aaaagaatat ctagaaaaag acccttatat cggtgcttca atcggacgta tggctggacg
    36121 gattacgaat ggcacattca aattagatga tgaaacgttc gaagtaaaaa cagacccgga
    36181 cactggtcat tgtttacatg gtggtgcacc tagctttgaa gccaagcagt ggcattatac
    36241 agtcatcaat ggtgaaaacg aagcatcggt cattttctat cagactagcc cagatatgga
    36301 aaacggattt cctggaactt tagatgtaga agtacggtat acgctgacga acgatgatac
    36361 atggcgagtg acggtccaag ggatcagtga ccgcaaaaca gtattcaatc ctacaaatca
    36421 tgtctatttc aatttaaccg gtgatccagc acagtcaatc gacgaacata tactttggtt
    36481 gaatagccga ttatttgcta cgctcaataa agatacaacg cctacaggag aagtagtttc
    36541 agtaagtggc acagcatttg acttccaacg tcctaaaaaa ttaggtgagg tctttgccag
    36601 tgattttcgc caatcaatga tagtgggcgg catcgatcat ccatttttgt tgaaaaaaca
    36661 atctggttta ctaaatgctg cggaattgac aagcccagat gaaaaagtat cgatccaagt
    36721 aaaaacagat gcttcaggaa tcgtgatttt tacagcaaat tttggagaag atggaccgga
    36781 aatgcgcggg aataaattag taaatcatgg cggtatcaca tttgagactc aagaactgcc
    36841 aggagcggag cgacttgaaa gttttggaga tgtcactctg gaacctaacc aagtacgcga
    36901 gtttgtaacg gaatacaaaa taaatataaa gaaatagaat aaattaaaaa gggagtgtga
    36961 cagtgtcaca tccccttttt aatttattct atttctttaa aggcagatca agccagaatg
    37021 atttgtctaa acttaacaag gttagtacag gaacagttgc agctttaact gtacgagtag
    37081 ttgtttctgg tatctgtctt gccgtaaaat tgttccacaa atcccagatt gaagcgacac
    37141 ggaagatgga agaagcgttg ttgacgcgtt ttatcgttga aaattctaat cctgtgattg
    37201 gcacgccatc tgttgtccat ttgattggca catcgaatcg cttagacata ttgaggattt
    37261 cagcgtttgc ttcttcagtt gttgctgctt cttttttcgg ttcatcatat tttgtcaggt
    37321 cgtacgcttc gatgatcgcg ttcaattttt gtgaatattg gcgatctgta gcataacgtc
    37381 cttccaaata tttggttgct tctttgtagt cttttgtttc cgacttccaa gttggtttgt
    37441 agaaatcagg attgccagtc agtccatttt tgatcaagtc ggcatagtct tccaaacttt
    37501 ccttgtaaga aggatattta cggaattgtg cgcggatttg gtagctttgt ccggaactat
    37561 cttgttcaag tgtattgaag gtcacgctgc ttcctttata tgatcctttg ataccaaaaa
    37621 ggttatgatt tggttcgctt gataataagc tgtttccaga tccactttca aggatagctt
    37681 gagccatcat gactgatgcg tagattcctt ctttttgccc gatttcctgt gcatcatctg
    37741 caatcttttt caagaactga tcagttgttt ggttttgact gaaatggaac gagccatcta
    37801 atccatctaa aatagatgag ccgcctgtca gcgaacctga agcagcaggt aaacctgcta
    37861 agtttactcg gacagcaaaa tcaggcgtga aatctgaaat agaagttact cgaactttat
    37921 ctcctggttg aggcgcatgg ataaattgtc cgttcccaat atagatacct acatggtgag
    37981 taacaccgga agaaggtccc cagaagacga ggtcgccagg ttgggcctga cttactggaa
    38041 tctgcgtacc tgcatattgc tgttcgcctg tccagccacc gatataatga ccggttgctt
    38101 tcatatatac gtaataagtc aaaccagaac agtcaaaagt atttggacct tttgctcccc
    38161 atacatatgg ttttccaaga tgcttgaatg cttctcgaac gattgcttgt tggacattcg
    38221 ttgtatttgt aggcaataca gcatcaattg gtgattttac aacaaaatca gaagaagcag
    38281 aagatggatt ctctataccg ctttgatttg ctgtatttgg acgagatgaa gcattgttcg
    38341 ttccgccatt cgatgaacca ttgtttggat ggtttggttc tctctgagta tttccattgt
    38401 tattcgatcc agatgggttt gtttgattcg aagaattccc gctgttcgaa ttattgttat
    38461 tattgtttga attatttgaa ccattattat tgttgttatt acccacatct ggtttgtttg
    38521 gcgtttccgg atctggtgta ctagggactt cttcactagg atcagttggt ttctctgttg
    38581 aagtatccgg tccagcttta aattctgcag taatagataa attgttcgta cctactgtaa
    38641 aggtctttgc atcataattt acttctttgg caccattaac agcgactgaa gcaatttgtt
    38701 caccttcagc tggcgtaata gtgtaagtta ccttcgtttc cgctaataaa ccttctaccg
    38761 ttccactttc cgtaatatcc aaaggttgct gttcagggga atccgctgtc gaagcagtca
    38821 gttggatctt accaatcaaa gaaggagcaa ttgtaaggga atatgtctcg gctggctttt
    38881 cattttctga tggatcagcc ggatcaattt cagttggctc ggtcgatcct gttgttgagt
    38941 tagttgttcc agttgggtct tctgtaaaag aatccgttgt ttcactagta gctgagctgg
    39001 atgtagtcgt atctgttgtg actgtttgat cagctatttg attatctacg gcaaatacgg
    39061 tattggtcga caaaacagac ggagcaatca gccaagtagc agctagcaaa cgtatcgttt
    39121 ttttattttt cataataaac tagtcctttt cctttcattg attttctgtc ttattcagca
    39181 ttcattagac attttttgga acagataatc gtgtcgaaaa gctttttctt ttcataaatc
    39241 tgtctgtgta ttctaaataa aacaatttgt aaataagcaa cgagcattca ttcgtttttc
    39301 tttgacgttc attttattgc atagaccacc tttgtttggg gataaacaag tggggataaa
    39361 aatggttatg catatgtaaa ttataccaaa gtttggaaag tatcacaaaa atttataaaa
    39421 ataatgatcc taaaaagaaa aagactgaaa tatgtgataa acatctcata cttcagtctt
    39481 tttcattaac tattcataag acattaggtc ttcatcaaac ccgctaacga gagtagtgat
    39541 cattgctacg actagactaa cagccagtcc tgctaagtac cagaaaaaag taccggaaat
    39601 gaatgcaggc aatgtggtca gactaccaaa tacaaaagcg atcgctttca cttgggcaaa
    39661 tcccattaca gctccaccag cagctgcacc aatgcaagca gcaacaaatg gttttttcaa
    39721 tgggatcgca attccgtaaa cagttggttc ggtcactccc gcaagtaaaa tcgaagcaaa
    39781 agcagaaccg gagatcgaac gggtttttga acgtttactt cgcagaaata cacctaaagc
    39841 tgcaccagcc attccaaaat tggctgctcc tgtcaacgga acgattcggt cgaacccata
    39901 agcagagata ttactgatca tgacagggtt tacagcccat tggataccaa atattaccaa
    39961 aatattccaa ccaccagcga tgattgcgcc actgatcggt ccgttaatat ccatcatcca
    40021 agtaattgcg gcagctaaac cttcgccaag gtaaacaccc gcaggcccga ataccattaa
    40081 cgtcaaagga accgtgataa ctaatgaaag taaaggtaca aatactaatt gcatattttc
    40141 gcgaatatat tttttgagga atttttcaac aaaagaataa aagaaaatac ctaaaatcgc
    40201 cggcatgaca gtagacgtat actgcatcag tactacagga atccccagaa aatcagtagc
    40261 tgctttgcca ctgtctgaaa gtaaagaggt cagattcggt tcaatcagtg ctgcagcaat
    40321 gacagccgca atatatccat tcactttaaa cgttttggca gcagaaaaag caagaaagat
    40381 tggcagaaaa taaaagacgg cattcgacgc agcagacaaa atatgataag ttccgcttgt
    40441 ttcactaagc cagccaatcg tagtcgctaa aacaaccaga ccttttaata taccagaacc
    40501 ggcaaacagc ggaatgatcg gcgtaaagat ttccgatatc aaaccaaata gtttttcgat
    40561 cggatttagc ttttctttcg gagctgaatg atcgatttga cgcgtcaatt ccttgtaagc
    40621 aggagccaat ccctgatcca tgattatttc tgcatatcta tcgagtaaaa tgaaatactt
    40681 aatggaaggg atttgctgtt ttaatttttc tgcattggct actggctgtt ttttcaattt
    40741 gatacgaagc cgagtcgcac atggaaggac agaatcaata ttgtcttttc ctcccaactg
    40801 ttcaatcagc atatgaatct gctcggacag taccatctgt tcaggattca ttgtcgtttc
    40861 cattagaaga ccactccact cactaatata atattggcgt aaggtgtcat ttcgcctgta
    40921 cgaataaatg catgacagtt attcaactct tttttcattt cttcatgtgg gataaagcta
    40981 atcggtgtat ctggtaacaa taatttgatt ttttccaata tttccgggtt cattgtcttt
    41041 atttcttccg ccaattcaat cgattgtacc tcaagttctt ctaaaacgtt ttctaaaaca
    41101 gataaaaaac ttggaagacc gtttgagaca gccaaatcta ttttttcagt accaaagggg
    41161 acaggcatgc ctgcatcccc gatacttaat ttatcaaagt gccccatttg tgcgattact
    41221 cgtgaaatat ctgaattgat tacttttcct tttttcatgt ttgtttcttc ctctcattgt
    41281 taattttaga ctgctgattg ttctaaagct tcgcggtaag ggatagaagg ttgcgcacca
    41341 taagactgca cagcaacaga agaggctaga tttccataaa ggatcgcttc tttcaagtta
    41401 ctaaggtctg gctgaagtgt gctgcttaat gcgccaataa atgtatcacc tgcggctgtt
    41461 gtgtcaacgg catccacttt aaaagcaggg acgataccgt gttctttttc tgtatgatag
    41521 aaagcgccgg cactgcctag tgtaatgatt actgttccaa tacctaattc atggagtttt
    41581 tcggctgctg cgaccaaact gttatgatct gtcactctga ttcctgtaat gatttctgtt
    41641 tctgtttcat ttggtataat gatatctgtc ttttcaagta gttcagtagg aatcgtttca
    41701 cgagctggtg caggattcag gatcgttgtt tttcctgcgt cttttgcaat agaaaaagct
    41761 aggatcgtcg catctaagct actttcaaac tgagcaataa taaagtcact gtttgtaatc
    41821 agttgcttgt tttttaggac ttcttgttca tggaaagcat tatttgcccc tgcatggatc
    41881 aagatactat tttctcctgc tgcgtctacc ataatcattg cttgtccggt tgtggcgcct
    41941 tggatttcag ctatcccaga tgtatcgatg ttttcttttg tcagtagatc taataactgt
    42001 tgtccttcgc tgtcagcccc aacgcctcca ataaaagatg tccgtgcgcc tgaacgtttg
    42061 gctgccactg cttggttggc acctttacct ccgccagcgt gaaaaatctc atgcgcatgc
    42121 atcgtttcgc ctggcttggg catgttggtt aaacgaaggg tagtgtccat attaatactt
    42181 ccaattactg taaccgtgtt cattttacaa acctccaact atctcattta ttttgatggg
    42241 aaaattattt tttaaaacgt tttagtaaaa cgttttaata gttcgataaa aatatagcac
    42301 accttgaaag ctatttcaag ggtgcagttg aaaatctttt ttctaatcgt acaggtaatc
    42361 ttttttcttc ccattctttt tctgggaatt ggatccgatc taacaataac ttggcagatg
    42421 tttgtcccaa ttcgaagatt ggttgtgcga tcgttgtcag tttcggcttg atatactcgc
    42481 acatatctat attatcatag ccaataatgc tgtagtcttc agggatgctt tttccagctt
    42541 cctctaatcc gcgatataat ccaaaagcta attcatcatt taaagcaaac acaccagttg
    42601 cagcagaatc aagaagttct gctgtgatct ggtagccacc ttgttttgag aactgtgtag
    42661 gtaagaggat cagttggtcg tgagggattt ggtaaacatc aagagcagat ttaaacccct
    42721 caatccgagc gtggacattt tccggtggat tttcaggata gacaagggcg atcgtttgat
    42781 gtcctagact aagcaagtgc atgcccgcta ggtaaccacc gcgaaaatcg tctgttcgta
    42841 cggcatcact aaatccttca gattttttct gatccaaaac aatgaagggc cggccttgtt
    42901 ttttcaaatt ttcgttgatt gcatctgtag agacagcaga agtcgcgata atgaatccat
    42961 ccacacctcg acgtgtgagc tcggctagat attcgatttc tttttgatga tcagaatcag
    43021 cattacataa tatcgtgacg aaattctgct tatacaagat atcctcaatt cctcgcatca
    43081 gtgtactgaa gaatggattc gtgatatcag gaacgagtac accaattgtt ttcgtttcct
    43141 tggtaatcat ttgtctagca aaatagtcag gctgatagcc taattcttct ttggcagcta
    43201 aaactttctg aacagttttc ggactgaatt tctccgtatt tccatttaat atcaaggaga
    43261 cagttgtgat tgaaacgccg gaaagtttag cgacttcttt gattgtgatc ttttttttca
    43321 ccatgttcat actcctatct aaatcctgtg gttattttcc catgaaatca aaaagaagta
    43381 aatgaaaatc acgtgtgcca gtacttattt gctttgttgg atagcttcga ttatttgttt
    43441 cgttgagcgt actcgtgcag ttttcggaaa tatctttttt atgaacaatt gatgggattc
    43501 tttgtcacga tcactgcaag catcttcagc aagcacgata tggtacccgt gttgaaaggc
    43561 atctaatgca gtagcatata caccgtttgt tgtcgctaca ccgcttaaaa tgatagtttc
    43621 gattccacgc cggcgaagtt gaagatcaag agaagtacca aaaaaagcac ttggattgta
    43681 ttttgtgata gtcaacatat tatctgtatc tttcaaagaa tccttcaaaa gtaaatcagt
    43741 gaattctgga ggtacagaag cagtcattcc acccatatca gtttgttgac tcaaataagg
    43801 gaaagtggag atgtctacgt tcactaaaac tttcaaggca gcggtacgtt tcaatgttct
    43861 tactaacaaa tcattgtttg tcagaataga tgctgcagtg tgtggagaga gtgtaccgtt
    43921 atttacgatc tctttttgca tgtcgatcgc gacaaaagca gtttttgata aatcaaaaat
    43981 catattttcc tttaaatcat tcatgatgtt ccttctttca aataacaatg ttttttatgt
    44041 aagtaaatca taaatcgttt atttgtcaac taaattaaag gattcctttc tttagtctag
    44101 agaaattcag tggtaaaata aataagtgtg agaaaacgca ttcgatgcat agatatagcg
    44161 agaaatatcg tgaaaatgga ggaatcagag tgaagtatgt cgaagtaaaa aatgaataca
    44221 aacgatacaa aatgggagaa acgaccatta cagcaaatga cgatatttct ttttcaattg
    44281 aaaaaggcga attagtgatt atacttggtc caagtggagc aggaaagtca acgattctta
    44341 atattttagg tgggatggat acacctgatg aaggtcagat cattattgat gacacagata
    44401 ttgcgcaatt ttcagataaa cagcttactg cttaccgacg aacagatgta gggttcgttt
    44461 tccaattcta caatctcgtt cctaatctaa cagcaaaaga aaatgtggaa ttggcaacgg
    44521 aagtttctcc tgatgcgctt gatcctgtag aggtattgag gcaagtgggc ctagctcatc
    44581 gattgaataa ctttccgtcc caattatcag gaggcgaaca gcagagggtt tcaattgctc
    44641 gtgcactagc taaaaatccc aaacttcttt tatgtgatga accgacaggc gcgctggatt
    44701 ttgaaacggg caagcaagta ttgaaattat tgcaaaacgc tagcagacaa cacggcaata
    44761 ccgttttgat cattacacat aattctgctt tagcgcccat cgctgataga gtcattcaca
    44821 ttaatgatgc taaagtaagg tctgttgaat taaatgatca tccttcatcc attgatgaga
    44881 ttgtatggta gggggatttt atgaaaacaa gaacatatct taaagcaggt tttcgcgaaa
    44941 ttcggcaatc taaaggacga ttcattgcaa tcatccttat tgtcatgcta ggaacacttt
    45001 tatttgtagg tgtcaaaact gctggacctg tcatgcaaaa aacgatggat cactatgttg
    45061 gtacagcagg actttctgat ttgcaaatcg tttctactgg tggcttgaca caaaaagata
    45121 ttgctcaagc agaaaagatc ccagatgcaa aagtagaaac aggaaaacag atctattatt
    45181 ccaatactgg caaaaacgaa gtgattcaaa tattttctta taacaaaaat aacaagcaga
    45241 acaagctaca attaactgat ggaaaactcc cggaaaaacc aaaccagctt gtattggatg
    45301 aaaaagcgga agatgaaggg tataaaatcg gtgatattta tcagattgat tcagatgaat
    45361 tgaaagaaaa agagtatacc atcacgggct ttgtccgttc tccattgttc atcaacaatt
    45421 tggagagagg atacgcgaac gtcggaaatg gtagcgtcga ttattttgtc tatttacctg
    45481 aatccgtttt taaaacagat atttattctg tcttatatct tgattttaca aacgtaaaga
    45541 atttgaatac gtacagcaaa gcttacaaag ataaaatgga gcaaaatcaa gaaaaggcag
    45601 aaaagtattt acaagatcga cccgaagaac gcttagaaga gctgaaaaaa tcggccaacg
    45661 aagaattaga accggcacag caaaaaatag cggatggcaa agcacaaaca acgcaagcaa
    45721 gaacacggtt agaagatgct aaaaaacagt tagcgcaaca gtcagcaact atccagcagc
    45781 ttcctgaagc gcaacgaaca atcgctgttc aaacattatc tcaacaagaa gctcaacttg
    45841 ctgaacaaga aaggcaatta gttgagaaag aacgtgaact ggcctctgca gaggaagaat
    45901 tgaatcgaaa tgcagaaaaa attgaaaatt taactaaacc atcataccta tacaatgaac
    45961 gttcagaaaa tgtcggtttt caagaatttg gtgatctgtc tgatcgtatc gctgcgattg
    46021 ccaatgtttt tcctgttttc tttttcttta ttgccgcatt gatcaccttt acgacaatga
    46081 cacgtatggt agaagaaaac cgcagggaaa tcgggacatt aaaggcgtta ggttatacga
    46141 aagttgaaat cgcaaaaaaa tatgcaatct atgcaagtct agcctcaggg ataggtattg
    46201 ttttaggaac gatattagga acgaatcttt tgccaaggat catttttgag ctgtctaacg
    46261 agcggtatga tattgggagt gcgcttattt tttatgactg gccgccgatt ttacaagcag
    46321 ctgccgcatt cttcattgcc gcatttggcg ctgcaatgat cgttttattc aaagatttaa
    46381 gagaacggcc tgctgcattg cttcaaccga aagcaccgaa gccagggaaa cgcatttttc
    46441 ttgaatacat cactccgctg tggtcacgcc ttagctttaa tcaaaaaatc agttaccgaa
    46501 atctcttccg atataagtca agaatgctta tggcgattat cggaattgct ggatgtgccg
    46561 ggctgatggt tgcaggcgtg ggattgaaag attctctaag ttctgtttca gccaaacaat
    46621 ttggaccgat cattgactat caagcaatcg ttacattgga ttcatccacg gaagatagtc
    46681 gtcaaaaagc agcggatatg ctgcagaagc aagaaaaagt aaatgagatc ctagctattc
    46741 atactcaaac cgtagaattg agaaaaaaag gacatgcttc gcaaagtctg acagtgatcg
    46801 taccaagtaa aacagctgat ttggatcgtt ttatccattt aactgatctt gaagagaacc
    46861 cacgctcctt gcctaaaaca ggaatcgcta ttacgcaaaa agtagcagaa ctatttgact
    46921 tatctacagg agatcatatt tcgctttatg atgaagatca aaaagaatgg aaagtcgaag
    46981 taaaagaaat cgtgaaaaac tatctaggta gctttattta tatgtcccct gaatattatg
    47041 cttcagttac tgatcaatcg atgatggata atgcattttt gctgcgtaca gataagatga
    47101 caacaaaaga agaagaaatg ctttccgaga aactgcttgc cacagacgct gtgacaaata
    47161 cgtctttcat gtccaaacaa atcgaaacgc aagaagaatc aatggcgaac ttagactcgg
    47221 ttgtcttaat ctttgtcgta ttatcaggac tgctggcttt tatcgtcttg tataatctaa
    47281 ctaatatcaa tatttccgag cgtgtacgcg aattatctac aataaaagtc cttggatttt
    47341 ttgataaaga agtcacgatg tacattgttc gagaaaatat catctttact cttttaggaa
    47401 tcatcggagg atttggtgta ggatatatat tgactgactt catcttgcaa caagcgtcta
    47461 tggagaatgt catctttcca ttagtgatca catggtgggc ttatgtctta tcagcaggat
    47521 taactatcgt atttacggtt attgtgatga ttgttaccca tttcaaatta aagcatatcg
    47581 atatgattga tgcgttgaaa tcgaatgaat aaaaacagag gttgtgaaat cagcggtttg
    47641 acctgagtat tagagaaaca aacggcaaaa tagtttttca tattttgccg ttttttgagc
    47701 taataccgaa ggacagctga ttgaacacca tgaatcagag gttgtaaaaa agccgtcctg
    47761 catcgagcaa aaagaatact caaataaaat tagctcctca tgttttacat ttgagaagac
    47821 tgattgccgt agataaaagc ttttgaacac catttataga agaaacaaga atattttgct
    47881 attttaaaca agaggataga caaatacatg tctgatctct tgttttttat tgggcaacag
    47941 acctcttaga atgtataaag ataaatttta caaacgcttt cggttcagtt ataataataa
    48001 atgataactt gtatatataa gttgtggtag gtaataaatc agttgaatgg aggtaaatat
    48061 ccatgacatt taaagacaaa gtcatttacc aaatctatcc caaatcttac aaagacagta
    48121 atggtgatgg aattggagat ttaaaaggaa tcaaagaaaa actcccgtat ctgaatgatc
    48181 taggcatcga catgatttgg ctaaacccta tttatccaag ccctcaaaaa gacaacggtt
    48241 atgatatctc tgattacaca gccatcgatc ctatctttgg tacgatggag gaattcgaac
    48301 agctaatagc cgaagcaaag aaataccgga tagaaatcat gctcgatatg gtgctgaatc
    48361 acgtttcgac agaacatgaa tggttcaaaa aagcactagc aggggaccag cattatcagg
    48421 acttcttcat tttaagagaa gagccaacgg attgggtatc aaaatttggt ggaagtgctt
    48481 ggtcgccatt tggagatacg gggaaatatt atttgcactt atatgatcgg acacaagctg
    48541 acttgaactg gcggaaccaa gcagtaagag aggaactatt caaagttgtt cgtttttgga
    48601 tggaaaaagg ggtaaaagga ttccgtttcg atgttatcaa tgttatcggt aaagacgaac
    48661 aattgaaaaa taataaagaa aatgatggca aacctgaata tacggacaaa ccaatcaccc
    48721 atacgtatct aaaagaattg aaccaagcaa cgtttgggac ggattccact atcatcacag
    48781 tcggtgaaat gagctctacg acgatcgaaa attgtatctt gtatacagaa cctagtagag
    48841 aagaattatc aatggtcttt aatttccatc atttgaaagt cgattatgaa aatgggaata
    48901 aatggtctac tcctccattt gattttgcag aattaaagcg actatttcat acatggggag
    48961 aaaagatgag tgagggtaac ggctggaatg ctctattttt taataatcat gatcagccac
    49021 gtgcgctcaa tcgatttgta gctatcgatc agtatcgaaa acaaggagca gagatgctag
    49081 caaccatgat ccatttgaac cgtgggacgc cctatattta tatgggagaa gaaatcggca
    49141 tgatcgaccc agactattca tcgatttccg actatattga cgtagaaagt ttgaatgctt
    49201 ataagttact ccttgaacaa ggattcacag aaaaagaagc gtttcgtcgg atcaaagcaa
    49261 aatctcgcga taactctcga acacccatgc aatggtcaga cacagaacaa gcaggattta
    49321 ctacagggaa accttggtta aaagttggag gcaagctaga agagattaat gtagaaaaag
    49381 aactatcttc tgaagattct attttgtcct attacaagaa attgattcgg ctgagaaaaa
    49441 catatccaat cgttgcacaa ggagattatc atgcttatga agcaaatcat ccggaagttt
    49501 atggctttct tcgccaattt gaaggacagc agctgcttgt attaacaaat ttctatgata
    49561 aagatacaag gatcgccatc ccagaaggat tcacagaagc caatattctt atcgataatt
    49621 attccgatac tgttcttcat caaagtctgg agctaaaacc ataccaggca attgctttgc
    49681 tgaaaaatta aaacaaaaac acaaaacgtc gttcctacga gctattctcg taagatcgac
    49741 gttttgtgtt ttatttaaaa taatcttctt cagaaaatga agagttttca tactctcctt
    49801 tatcgccata aaatttattg tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta
    49861 atttgactaa cgtatagata ggaataccaa aaataacgcc cacaattccc attagatcac
    49921 ccatgacaag taaaacgatt aagatcgtaa tcggatggat ctgtaaacga tcacccatta
    49981 ctcgaggaat caccaagtca ccgtggagaa gttgtacggc aaaccagaca atgacaaatt
    50041 tgacagccat aaaagtagag tcttggaatg caatgatcaa acctggaaag aaggcgataa
    50101 acggcccaat atatggaatg atacataaca acccagcaga aatagctaaa acactagcaa
    50161 attctaatcc gatcagtaag aaacaaaccc agtagactgc tccaaggata aatgaagcaa
    50221 tgatttgccc ctttagaaat gaacccaatt gttgattagc aatctttgtc aaatttttaa
    50281 aatctgtacg aaatgctggt gggacaatcg ctaaaactga ctgataaaat ttttgaggat
    50341 ctctcaaaag gaaaaaagcg acaattggac cagtaaataa agtaataaaa atagctgtaa
    50401 cagttgaaaa aatgttgctg atccgttgtg cgccactttc ccaataattt ccaataaatc
    50461 cagctatatc agacgtcacg tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa
    50521 aaggtgtttt ctccaaaaat tgtcggagac tctcttgaaa atcttgaaac aaatctggca
    50581 aactactgat caatgtcttt gcctgttctt caagaaaagg aataagccat atgcttccaa
    50641 gccccaataa aaataaaata atcacataaa cgataatgac tgacaagttc ctcgaaaggt
    50701 gcttgctcaa acgtttgatg actggttcca aaagataata caagatcagt gcaaacaata
    50761 atgggggcaa gatagtagcg ataatgattc cgacaggctt gaatataaac gagatctgat
    50821 ggaaaaaata aatgatcagc gcaagtaaga taactattcc taaaacatag taagaggttt
    50881 ttcctcctaa aaagcggatc aaacgattcg ttgtatctgt ttcttttttc actctttttc
    50941 ctcctttatc tataacttca ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt
    51001 tgattgcaaa aatatcgtca atcattcata taaactttct catcaattaa aataagaaaa
    51061 cgcttgacag ttcttttaac catatgataa attaaatcct gttgctaaaa cgtaactatt
    51121 acggtttgcg aaaagcgaat cggaacattc aaatgaaaga agatgagaag aggagtgtca
    51181 taaatgaaac gattgatagg tatattggcc atgctggttt tagcaggtat gttaactgct
    51241 tgcggggcaa gtgggaaagc cgaggacagt aaagaaaaat tgagcgtgat gacgaccttt
    51301 tatcccatgt atgattttac aaaagcaatc gtcggggatg aaggagaagt agagttattg
    51361 attccagcag ggactgattc ccacgattat gaaccttctg ctaaagacat ggcaaagatc
    51421 caagatacag atatctttgt ttataatgat gaaaacatgg aaacatgggt ccccgcaatc
    51481 caaaaaacat tacaagaagg caatgtccat acgataaaag cgacagaagg tatgctttta
    51541 cttccgggaa gtgaagaagg acatgatcat gaccatgaac acggggaaga aggccataca
    51601 catgagctag acccacatgt atggctagct ccaagtttag caatcaagca ggtagcaaac
    51661 atacgtgatc agctgattga agcttaccca gaaaaacagg aagtatggac aaaaaatgca
    51721 gcagcctata ccgagaaact tcaagcactc catcagcttt atcaagaaac ctttaagcaa
    51781 gcaaaacaaa gatcttttgt cacgcaacat gctgcattca actatctagc gttagaatat
    51841 ggattgaatc aagtatcgat tgctggtctt tcttctagtg aagaaccgtc tgcggcgaga
    51901 attgctgaac tgaaacactt tgttaaagaa catggaatca actatattta ttttgaagaa
    51961 aacgccaaag atagtattgc cagaacgtta gcaaacgaag caggtgtatc tttagaagta
    52021 ttgaatcctt tggaaggatt gacgaatgaa caaatagaaa atggagaaaa ctatctttct
    52081 atcatggaag caaacctcga agcgttgaaa aaaacgacag agacagaaaa cccgctagaa
    52141 gatactttga cccccaaaaa agaaaagagc gtctacaacg gttattttga agacgaggat
    52201 gtacaagatc gctctctttc agattgggca ggaacttggc aaacagtaga tacgtatgta
    52261 gaagatggga cttttgatcc tgtttttgaa tataaagaaa aattgaatca ggataagaca
    52321 gcacaagaat ataaagaata ttatacgaag ggctatcaaa cagatattgc ttcaatcaag
    52381 atcagtgaga aagagatggc atttacgttt aagaatggaa caacgaaacg ttcaaattat
    52441 cgttatgtag ggaaagaaat cttgacctac gaggcaggaa accgtggcgt aaggtatcta
    52501 ttcgaagcag aagataagga aagtggcgct tttagatata ttcaattcag tgatcacacg
    52561 attgcaccgg ctgactccga acattttcat ttatatgcag gaaatgaaag tcaagaagta
    52621 ttgctgaaag aaatggagaa ttggccaaca ttttatccag atacaatgga tggacgtgaa
    52681 attgcacagg aaatgctttt gcactgatag gcaaaaaaga accgaagggt ttggatcggc
    52741 tggtcattta gtcggtccaa actattcggt tctttttatg aaatcaattt ctttaatcca
    52801 cggatagagg ctgggatggt ctcgccgttt gccaacgtga cttctctggt ttttttatta
    52861 atcgaccgca cattttgtgt attgataaca taggatttat gacaacggat aaaattccga
    52921 tgcattttct cgatttcttt gatcttctca tagaattgta attgggcatg agtcaaatgg
    52981 acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat attggatgtc tttgatcggt
    53041 aaaaatttga tctcatcatt aaactctatt ttgaagatat ctttttggat ttgggacatt
    53101 tgaaaatctt cgaaaatttt gctcaggata tgctcgacat tttcttttaa atcataaaga
    53161 tcttctttga cgatataatc agtcggttcg atgttgcttt ttaagatttt catcgacatt
    53221 tccgtatggg aagtcacata gattattttt cctaaaggat ctaattcacg gatcaattta
    53281 ccgatcgcta caccatccat ttcactgtct tctagttcga tatctaaaaa gtaaatacca
    53341 aaagaagaag ttctgatctt ggctgcattg agaagttctt ccggatcaga ggtagaaata
    53401 tggacataac tgtctaagtt ttcaatcatg attctatttt tgatcaccgt tgataaaact
    53461 tctaattgtt ttggatcgtc ttcacaaata taaatgggaa tcatatcatt actcctttat
    53521 tttatttgta tataaatcgt ttgagaaaaa cgctggtttt caattgatgt ttcaaggaaa
    53581 agataaggat tggtctttgt caattcatcc aagataaaca agccaaggcc ttcgtttttt
    53641 ggatttgttt ttgttgagaa ccctttttgt ttcaactggt ttaacggtaa tgtatttgtt
    53701 gttgtattga tcacacgaat catcacggaa tgattcttct tgagtatggt caattcgatt
    53761 tttttcttct ttgcagcact agctccttca atcgcattgt ccaagataat accaagcata
    53821 cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg gctcaggaaa atgaagctgg
    53881 acgatcaacc ctttttcctg tgccatcatg agttttaatg acagtaaact acggatctca
    53941 ggaactttca tattatccaa catacttaga ttggccggaa aaaggttcat catctggcgt
    54001 gtaggtaata tcgtttgatc ataataagac cgtaatcctt ctaagtcgcc ttctgaaata
    54061 tagctgtgaa gagaaaaaag cagattttgg taatcatgtt tgaattctct caagtcttga
    54121 tagttttttt ctagatcttt gatataatcc tgttgcatcc tgacttgttg ctctaataac
    54181 tgatcagcta atttcttatt agagattttc caataataaa ccaagatgat caggccaaca
    54241 cacagatata gaatgaaaaa actcgtattc aaagctaaga catcaggtgt ttcaccagaa
    54301 aaacgggtat aaaaaataat gatatagtat gtaaataata tcaatgtccc gatcgtaccg
    54361 atcatacggc ggtccacagt gtgaaaccaa gtctctaacg atttttttaa acaaaaagct
    54421 aataaagcag ataaaaaagc ggataagaca atgtgaaaat actgcaatga atctcctggt
    54481 tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc ttgttaagtg atctcctaaa
    54541 agcgacgtca ataacccaaa aggaagcgcg taaaccaatt gttgacgcga agtcgaaaag
    54601 aagaaaaaag taagcatcag cattcctata gcaattagag cagtaaaaat ccctaaaatc
    54661 gaatagaccc atccaatcaa aatacaagaa atcagaatga ctcccgcaaa cagatatttg
    54721 tatcggatgt gatggtcaat caataagata caaattgtgt aaatcaaata ttgcaacgct
    54781 atggaccata aaaacataaa gtacagctcc cattgatttt taccaaatta tagcacaatt
    54841 ggcgtattat ttttttaatt tttcagggat tttgaattca gaactcatac caaagcagca
    54901 gccagataca aaaaagtcag ataagttagt taattgttta atgattgttt ttttcatgaa
    54961 agattcctcc aaaattcatc aagtagtttg taataggaag caacgtagct atttctaaaa
    55021 caatcccaag tagcaaatag gtagaaaagt aagaagctga gaaaaataac gtaatgagag
    55081 aaaaaattag aaacatgctg atcgccttct ttaataatgg gggacgtttc tcgggttcaa
    55141 tgggattgat tgctgtacct cttgatccat agcgaagcag taacaaaaag cagctgatta
    55201 acaaaaagac catcattggg aaggagatag tcaattgcag atgtttcatc accaagggaa
    55261 cgccccataa ataaagtagt gtccacagca gacaaacgaa ttcagagtcg gaatgcaatc
    55321 cataagcata tctgcggatg cagatataag aaagaaaggt gattcctgtt tccgcccatg
    55381 agcctgtcac cagagaaaag actagaacga ccgctagttt tccaagatta ttcaataaaa
    55441 cttctaagac aaattttact tggacataca ccatatcttc ttccgatttg tctccgctga
    55501 ataaggcttc taataatttg ttcacaactt tatctttcat ctcatacgta cctccaccac
    55561 aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc ataaaaagtg gttttgacat
    55621 caaaaccgtc atgatctcct aaaaaaatct tcgttttcca agaaccttac caaaaaaagt
    55681 gacagttttg atacgaaaag tactttttat gacaagtttg tgctttttcg agtgatttat
    55741 gttaaaaaat tactgacctt ataaaaggtg atggagggat tcttatggaa aatgaacaat
    55801 tgtttgaagt cggcgaaaca tatgcattta aatctgattt atttgaatca ccaataaaag
    55861 gaaaaataga aaaaatctat gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag
    55921 aagacaatga taccgctcat aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa
    55981 tagaagaata aaaacagata aaatgttgag agcataaaga ggttgtgagt ctcccgttta
    56041 gctctgagtt acaaggaata atgttaaaaa acagcttttc atgttttatg aatattgcct
    56101 tgtgaccgag gtgttggcag gagaatactg tttatttgaa aaaagggctg cgacaagact
    56161 tttgtcacag cctttatttt catgaatagg agttactttt ggctactttt agagaagtag
    56221 cttttaagga gcagataaag tcaaggcttt tatttctata aatgaagtag taaaatgaag
    56281 aacagatttt attacaggaa ggatgccgat aattgtgatc attgttgcag gaacgatcgg
    56341 tgcagggaaa agcacactaa ctgaaatgct tgcccaagat ttagaaacaa aaccttttta
    56401 tgagaacgtg gaagataatg aagtattgcc gctattttat tctaatccag aaaagtatac
    56461 ttttttatta cagattttct ttttgaacaa acgctttttg gcaatcaaag atgctttcag
    56521 ccatgatgac aacgtactag accgctccat ctacgaagat agtatgctgt ttcatttgaa
    56581 tgcagattta gggcgtgttt ctgaagtaga agtaaagcag tatgaaggtc ttttagaaac
    56641 gatgctgaag gagttagaag aaatttcgcc acaaaagaaa ccagatctac tggtttatat
    56701 tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa agaggccggg aatatgaaca
    56761 gctagaacaa gatcctgaac tctattccta ctataaagaa ctcaatcgac gatacgaaga
    56821 gtggtatgaa cagtttgata tatgtcccaa aatagtgatc gatggggaca aatatgattt
    56881 cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa atcaagatgc gggcaaagaa
    56941 aatgacagaa gaggcagatg cccagtcgat ccgctacact acaaataacc cgaaagcaaa
    57001 gggaatcttt acaactaaat aatataaaaa ctaagcaaat tgctcactcg cagatatttc
    57061 tctgtgagca gcatcttgct tagttttttt gattcattga gatcaaatag tgaaccgatt
    57121 cgtatgataa aattgccata aatgaaatgt ctgagtgagg gccatgatca aaaataaaat
    57181 ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga cagaaaacaa ggacaatagc
    57241 tcctgcgata aaggcaagaa cgacacttag atagtggaaa aatcgatcaa aatctgcttt
    57301 ttctttagaa atacaataat catataagtt cgttgctacc ccttttaaat ttccagttgt
    57361 aaataaatta gtgtaggcac gcccgttgat tttatcaaaa gctacccact ggatcgatgc
    57421 agtaaaagca atcaaaatcg ttactaatgc gggatgctca tggacgggtg tcaaactaac
    57481 aaagaaaaaa atcactgctt cataatatag aacaaacaag cgccagaaat gtttttcttt
    57541 ttttctaaaa taatcaatta gatactttgt tagaagaatc cctaagaaaa agaaaagggt
    57601 agacaatact tttttaccaa cacttagcca ttctccgtca aatgcttgga tgatcgatag
    57661 aatgatattg ccagtttgag cagaggcaaa agcaccatat tggatataag tataactgtc
    57721 catggctcct ccgataaatg tcagcaaaag tcctattgta cgcccctcgt gaaaggaagt
    57781 ctgcattttt ttcatcacct tttatgaata tttttgtctt ccttatctta gcataaacga
    57841 ttgaaaaaaa aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt
    57901 ttagaaaggt ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg
    57961 aaataaacaa attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa
    58021 attctccgat tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa
    58081 gtctttcggc tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc
    58141 ggacttattg aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa
    58201 atgatggaga aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat
    58261 gatatgaaga aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc
    58321 tgtgcaaaag ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg
    58381 atcactttta caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag
    58441 agaaaaaccg ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa
    58501 atttgtcgaa aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat
    58561 actgcacaaa aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata
    58621 tttgatcaat atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac
    58681 ctttcagtag aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat
    58741 gcacgacagc cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa
    58801 agaagccagc aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag
    58861 aatgaggaga tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt
    58921 caagatacta atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc
    58981 aagttgattg ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca
    59041 gcatacatca agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat
    59101 tatcgttctc gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat
    59161 gatcggatac caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat
    59221 ttgttttctg atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt
    59281 caaaaaaatg ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag
    59341 cagcttgtcc aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca
    59401 tatgaaggta tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg
    59461 caaacagcac tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg
    59521 atggcagact gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa
    59581 aatatagccg ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca
    59641 gaaatccgag agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg
    59701 cttgttcaaa gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa
    59761 gaatggatac gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg
    59821 caagaaaccc aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga
    59881 aaacttgatg cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat
    59941 gtctttttga ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa
    60001 gtaatcgact ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga
    60061 ctattttatg ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg
    60121 aaaaattcaa gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa
    60181 aaaggaaagt gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg
    60241 aatgtacgaa aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta
    60301 tttctggaac acagtttatt cggaaa
//
LOCUS       DAYYAO010000006        55264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000006 DAYYAO010000000
VERSION     DAYYAO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.006"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..946
                     /locus_tag="ACHARI_000301"
     CDS             <1..946
                     /locus_tag="ACHARI_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8441384.1"
                     /translation="QGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQA
                     PTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQD
                     ANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVV
                     ELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGL
                     APGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSS
                     GNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1299..2147)
                     /locus_tag="ACHARI_000302"
                     /pseudo
     CDS             complement(1299..2147)
                     /locus_tag="ACHARI_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2394..3587)
                     /locus_tag="ACHARI_000303"
     CDS             complement(2394..3587)
                     /locus_tag="ACHARI_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8441385.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3671..3871)
                     /locus_tag="ACHARI_000304"
     CDS             complement(3671..3871)
                     /locus_tag="ACHARI_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8441386.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(4295..4528)
                     /locus_tag="ACHARI_000305"
     CDS             complement(4295..4528)
                     /locus_tag="ACHARI_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441387.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4535..4966)
                     /locus_tag="ACHARI_000306"
     CDS             complement(4535..4966)
                     /locus_tag="ACHARI_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8441388.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            5315..5488
                     /locus_tag="ACHARI_000307"
     CDS             5315..5488
                     /locus_tag="ACHARI_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441389.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(5554..6162)
                     /locus_tag="ACHARI_000308"
     CDS             complement(5554..6162)
                     /locus_tag="ACHARI_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8441390.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(6168..7196)
                     /gene="vanB"
                     /locus_tag="ACHARI_000309"
     CDS             complement(6168..7196)
                     /gene="vanB"
                     /locus_tag="ACHARI_000309"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8441391.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            complement(7189..8160)
                     /locus_tag="ACHARI_000310"
     CDS             complement(7189..8160)
                     /locus_tag="ACHARI_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8441392.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(8157..8984)
                     /gene="vanW-B"
                     /locus_tag="ACHARI_000311"
     CDS             complement(8157..8984)
                     /gene="vanW-B"
                     /locus_tag="ACHARI_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM8441393.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(9002..9808)
                     /locus_tag="ACHARI_000312"
     CDS             complement(9002..9808)
                     /locus_tag="ACHARI_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8441394.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(9979..11322)
                     /gene="vanS-B"
                     /locus_tag="ACHARI_000313"
     CDS             complement(9979..11322)
                     /gene="vanS-B"
                     /locus_tag="ACHARI_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8441395.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(11325..11984)
                     /gene="vanR-B"
                     /locus_tag="ACHARI_000314"
     CDS             complement(11325..11984)
                     /gene="vanR-B"
                     /locus_tag="ACHARI_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8441396.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(12602..12769)
                     /locus_tag="ACHARI_000315"
     CDS             complement(12602..12769)
                     /locus_tag="ACHARI_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441397.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(12927..13112)
                     /locus_tag="ACHARI_000316"
     CDS             complement(12927..13112)
                     /locus_tag="ACHARI_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8441398.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            13257..13628
                     /locus_tag="ACHARI_000317"
     CDS             13257..13628
                     /locus_tag="ACHARI_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8441399.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            13886..14215
                     /gene="mobC"
                     /locus_tag="ACHARI_000318"
     CDS             13886..14215
                     /gene="mobC"
                     /locus_tag="ACHARI_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8441400.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            14176..15504
                     /locus_tag="ACHARI_000319"
     CDS             14176..15504
                     /locus_tag="ACHARI_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8441401.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            15501..15872
                     /locus_tag="ACHARI_000320"
     CDS             15501..15872
                     /locus_tag="ACHARI_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8441402.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            16055..16291
                     /locus_tag="ACHARI_000321"
     CDS             16055..16291
                     /locus_tag="ACHARI_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441403.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(16591..16794)
                     /locus_tag="ACHARI_000322"
     CDS             complement(16591..16794)
                     /locus_tag="ACHARI_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8441404.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(16798..17742)
                     /locus_tag="ACHARI_000323"
     CDS             complement(16798..17742)
                     /locus_tag="ACHARI_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441405.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(17743..21663)
                     /locus_tag="ACHARI_000324"
     CDS             complement(17743..21663)
                     /locus_tag="ACHARI_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8441406.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(21812..23893)
                     /locus_tag="ACHARI_000325"
     CDS             complement(21812..23893)
                     /locus_tag="ACHARI_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8441407.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(23890..25119)
                     /locus_tag="ACHARI_000326"
     CDS             complement(23890..25119)
                     /locus_tag="ACHARI_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8441408.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(25109..25360)
                     /locus_tag="ACHARI_000327"
     CDS             complement(25109..25360)
                     /locus_tag="ACHARI_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8441409.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(25383..27374)
                     /locus_tag="ACHARI_000328"
     CDS             complement(25383..27374)
                     /locus_tag="ACHARI_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8441410.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(27406..29805)
                     /locus_tag="ACHARI_000329"
     CDS             complement(27406..29805)
                     /locus_tag="ACHARI_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8441411.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(29735..30157)
                     /locus_tag="ACHARI_000330"
     CDS             complement(29735..30157)
                     /locus_tag="ACHARI_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8441412.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(30171..30707)
                     /locus_tag="ACHARI_000331"
     CDS             complement(30171..30707)
                     /locus_tag="ACHARI_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8441413.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(30744..31607)
                     /locus_tag="ACHARI_000332"
     CDS             complement(30744..31607)
                     /locus_tag="ACHARI_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8441414.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(31699..31914)
                     /locus_tag="ACHARI_000333"
     CDS             complement(31699..31914)
                     /locus_tag="ACHARI_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8441415.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(31991..33799)
                     /locus_tag="ACHARI_000334"
     CDS             complement(31991..33799)
                     /locus_tag="ACHARI_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8441416.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(33796..34272)
                     /locus_tag="ACHARI_000335"
     CDS             complement(33796..34272)
                     /locus_tag="ACHARI_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8441417.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(34347..34559)
                     /locus_tag="ACHARI_000336"
     CDS             complement(34347..34559)
                     /locus_tag="ACHARI_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8441418.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(34581..35546)
                     /locus_tag="ACHARI_000337"
     CDS             complement(34581..35546)
                     /locus_tag="ACHARI_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8441419.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            36117..37229
                     /locus_tag="ACHARI_000338"
     CDS             36117..37229
                     /locus_tag="ACHARI_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8441420.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(37307..37600)
                     /locus_tag="ACHARI_000339"
     CDS             complement(37307..37600)
                     /locus_tag="ACHARI_000339"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8441421.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            37768..38082
                     /locus_tag="ACHARI_000340"
     CDS             37768..38082
                     /locus_tag="ACHARI_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8441422.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            38103..40082
                     /locus_tag="ACHARI_000341"
     CDS             38103..40082
                     /locus_tag="ACHARI_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8441423.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            40095..40421
                     /locus_tag="ACHARI_000342"
     CDS             40095..40421
                     /locus_tag="ACHARI_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8441424.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            40437..40955
                     /locus_tag="ACHARI_000343"
     CDS             40437..40955
                     /locus_tag="ACHARI_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441425.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            40955..42307
                     /gene="celB"
                     /locus_tag="ACHARI_000344"
     CDS             40955..42307
                     /gene="celB"
                     /locus_tag="ACHARI_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8441426.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(42391..43896)
                     /locus_tag="ACHARI_000345"
     CDS             complement(42391..43896)
                     /locus_tag="ACHARI_000345"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8441427.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(43988..44578)
                     /locus_tag="ACHARI_000346"
     CDS             complement(43988..44578)
                     /locus_tag="ACHARI_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8441428.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            44757..46403
                     /locus_tag="ACHARI_000347"
     CDS             44757..46403
                     /locus_tag="ACHARI_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8441429.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            46452..47171
                     /locus_tag="ACHARI_000348"
     CDS             46452..47171
                     /locus_tag="ACHARI_000348"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8441430.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            47650..48597
                     /locus_tag="ACHARI_000349"
     CDS             47650..48597
                     /locus_tag="ACHARI_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441431.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            48609..49529
                     /locus_tag="ACHARI_000350"
     CDS             48609..49529
                     /locus_tag="ACHARI_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8441432.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            49570..51027
                     /locus_tag="ACHARI_000351"
     CDS             49570..51027
                     /locus_tag="ACHARI_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8441433.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            51372..51986
                     /locus_tag="ACHARI_000352"
     CDS             51372..51986
                     /locus_tag="ACHARI_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8441434.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            51979..53703
                     /locus_tag="ACHARI_000353"
     CDS             51979..53703
                     /locus_tag="ACHARI_000353"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8441435.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            53700..55151
                     /locus_tag="ACHARI_000354"
     CDS             53700..55151
                     /locus_tag="ACHARI_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8441436.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT        14274 a        12848 c        13009 g        15133 t
ORIGIN      
        1 gcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggtctaac
       61 tacaggagaa gatggaaaac tggcaattga cggtttagca ccaggagcat atcagctggt
      121 agaaactcaa gcgccaacgg ggtatgaatt agatgcgaca ccgatcgagt ttgagatcga
      181 aagaagtcag acagcggtgg tagaattgac gaaagcaaac cgacttacac caggcggtgt
      241 cgtgttgaca aagatcgatg accaaagcgg ggaagtcctg caaggagctg tttttgagtt
      301 acaagatgcc aatggaaccg ttctgcagtc aggtctaacc acaggagcag atggaaaact
      361 ggcgattgac ggcttagcac caggagtgta tcagctggta gaaacccaag cgccaacggg
      421 gtatgaattg gatgcgacac caatcgagtt tgagatcgaa agaagtcaga cagcggtggt
      481 agaattgacg aaagaaaatc gacttacacc aggcggtgtc gtgttgacaa agatcgatga
      541 ccaaagcgga gaagtcctgc aaggagctgt ttttgagtta caagatgcca atggaaccgt
      601 tctgcagtca ggtctaacca caggagcaga tggaaaactg gcgatagatg gcttagcacc
      661 aggagcatat cagctggtag aaactcaagc gccaacgggg tatgaattgg atgcgacacc
      721 agtaactttc aagatcgaaa aagaacaaga agtggcgatc tttttaacaa aagagaatag
      781 aaaagttgct gatagctcag atataagagg aaacaatcca acaagcagtg gaaataagca
      841 tcttccaaaa actggagaaa ccatcattag ccagtttata ttgtcaatat tgggtgtgct
      901 acttgttttc atcagtataa aatttagaaa gaaacgaaat atatgagcta aaataaaaaa
      961 ttgaataagc atagttttta aaatcaacta gtacccatca attgaatgga aactaccgat
     1021 tttagaaggg gtatacaaac aaatattagt atcggagtag agaaggttga acccaaccag
     1081 taaataagta aagacaattt tgtgctgcgt tgagaagtga aaaataaacc tttgtagtaa
     1141 tactagttaa taaaaaatat acaaaaacac tgcgaaaatc attcttgagt ttcgcagtgt
     1201 tttttgccta atgactcaag tttattgggt aatactttga ttttctttct acaaataatc
     1261 agcgatcagt tgtaattttt tctctagttt taatggctct ttgtcaataa ggagaaaaac
     1321 aagtcgggtc ttttgtctcg gaaagcttca accaactcgt gaataaaggt tacgcctcct
     1381 tgaaaggcgt ggaaaaacca agtaatcgat caatcaccaa tttcgcattt ggtagaacat
     1441 gtttggtgag ctgaaagtag gtggcgttca tgtctgtaac taagaattct atttctttag
     1501 gattagtgca gctaaggaaa tagtttgtta atcgaggaat tttttgtgta ggtaaatttt
     1561 ctcgattaat ttttctgtct cgccatccgc ggaaataaaa ctcagtttat cctcaacgga
     1621 aacgtgagaa cgaaattcac aaccctcaac actcaagaca gaattctttt ggaagacttt
     1681 ggtaaatagc ttctcaactc tttcaatgtg cgaataacgg ttgtcaaaga tacctaacaa
     1741 cttttcgaaa taaaaaataa cgacactttt tcagtcagta aagaagtgat tttatgtctg
     1801 acatgatttg aaatcgagtg ccttggacgg ataaaataac tttgagcgtt ccaatggctt
     1861 cgacagtttt tacaggtata gcgctgcttt tttaggcgta taaccattgg catatgatta
     1921 tattattcaa agtggacaat ggtttccttc ttcttgtttt tcaccaccat taatttccta
     1981 tttccatcta ccacagcaga accacaattt ctacagacac atggagcagg gaagagaact
     2041 gcagcaacga tcaatgtttt tttcttctgt aaggtttcgt aagagacctc tgtaatcatc
     2101 aaatttttgt ctattgatct cagaattttt ttgatagaac cattcataaa gcatatcgtt
     2161 ctctcagttg tttattttgt ggtgatttaa tcatacaaga aaacgatatg ttttcatata
     2221 aaattatatg ggattttaga ttatcttttt agaagttctt gaatgtcggt atagcccgtg
     2281 tttccgggct tttccggcat ttttgatatt gttagatact tacaaatgtt cttattacct
     2341 ctcaccattt ccccctcaaa agtagtaaat ggagtagtaa aaccggcctt gtgtcaggct
     2401 gccagccgtt ccatttctgc ccttgcggaa tcgaatgtgg cgtgtgcata atagttcagc
     2461 gtcatggtga tattggaatg tcccataatg tactgcaagg ctttggggtt cattcccgca
     2521 ttggctaacc gggtgcagaa tgtatgacgc atagcatggg gcgtcgtcgt tttcggcaac
     2581 gcttcttcat ggctcttgtt gtacttcttc acaagcctgc caaacatccc gccataatct
     2641 accgccgtca tgggatagcc gttctgtttg agaaacagga aattggcata cccgtcaata
     2701 atgaacggct ttgcaccttt gcggtttttt atcactcgct ggaacgcctg atacacttcc
     2761 tcattcatgg ggatttttct gacgccgctt tgggtcttgg gttcgtcaat atagtaaccg
     2821 acgccggtat ttctcaaaag ctggtggctc acgatgatta cccggttttc aaaatccaaa
     2881 tctttgtccg tcagtccgca cagttccgaa atgcgaagcc ctgtccccag cagaataacg
     2941 attgcgtcat aatgcttttg gtaaacctta tcaccttgaa taaaggacag aaaactttct
     3001 tcctgtgctg gtgtaagagg cacctttggt tccgtatcgt cgtccagaac gtcactaagc
     3061 tggaagtcaa acgggttttt tcggatgcaa tcgtcctgta tggcggtgta aaaagccgct
     3121 ttcagggaac gcttgtcgtt attgatggtc ttataagaaa ggcccttttc tttcatccga
     3181 agcgcccatt cctttgcgtc ggacagcttc acactgtcaa tcgggcagct ccccagccga
     3241 tcttgttcca gaatacgcat cagccgttcc cggcccagtt tggtgttgtg cttcacattg
     3301 ccccggtgcc tgatctgctt ggcgtagagc tggcagacgg tcattttctt gccgatggtg
     3361 tctatcccgt cgtcaaggtc tttctggatt tctttctcct tttcccggag tgaaatatcc
     3421 tcccgtttac ctgccggggt cttatccgta ggcactaact tccatgagta aacaaactgc
     3481 ggttttccaa aggtatcaac atatttgtaa gcatatcgtc cgtcttttct ctggctctct
     3541 ccagaccgca aaacgcgatt tttgctgtct cgtctttttt ctgacatctc gtcatgctcc
     3601 tttccatgac ggaaagagcc gcatatatgc ttataaatta ttataacata tctgcggccc
     3661 tttttcacta ttagattgtg tccaaagtgt caattatttt ctcaaactgc ttgcgtttga
     3721 tctggatgcg gttgccattc ataatgaccc agccagaggc cggattttcc tcggccagct
     3781 tacgcaattt gttttctccg atacggaaat atttagacgc ttcctcaatc gtcagcgtgt
     3841 atttttccca aataggcaca tcatggttca taatgcagcc ccctttcaca aattcaaagg
     3901 tttgtgtgat ttaggggcat agacggacgg ctgctggcac agctccacgg gactttcacc
     3961 cccgcgtggt tctcacgcgg ccctacccat tgcctgcgac gcttttaacg ctcggactgt
     4021 ggctataagg gagtatcata gtcctgcctg tacgtcgtcg cccgcaagcc gccacgctca
     4081 cattgcggcg tggtgttcct cgctccgctt ttcccgtagg gaaaagtatc atggcgcacc
     4141 agccgtccgg ctcggcacag acagaatgtg tccgtatgcc ctatgatgcg ccgccgttat
     4201 cttgcgggca tatttatcaa ggtgcgatca ggccaccggg aaaacaccga aacggggaaa
     4261 gggtaagccg tttcatgctg ccggggccgg ttcgtcagtt cgtgacgata gcgtggatga
     4321 gcttgatttc cagacggcgc ttcatggcct cgtccagaca cacatgcgga tagccgtttt
     4381 cgtcatagag cgtccgggta cacagcttgt tcatgtaacc gctgtaatac tgcaaaacgc
     4441 gctctacggc tccggcatcc ccagcgcggg cggcctcgat caccgaaagg ggcaaaagct
     4501 caatgccctt gtagttcggg ttcttcatcc gcgtctaacc tcccttcggc atcaacgcct
     4561 ttagctgttt ccgcagctcc gataaggtat ttgtcctgtg ccgctgaacg gcactccggg
     4621 acattcccac aaggccgccg atctcaccat ctgccatatc cagaacacag tgcaggatta
     4681 aaatactctg ctccatgctg ggcagcgcgg caaaggcttc ggcaacaagc tcattgttga
     4741 tatgtaggtc atagccatgc gacgaaaacg taaagctgtc gctggggtag tgatctactg
     4801 tacagagctt gtccaaatct gcctgtgaca aagagcttag tgacacttca cggtcacggc
     4861 ggcgcttcat ttcagccaag tagcttttgg cctcgttacg cagtaccgtc ttgcaaaaag
     4921 catcgaacat gcaccgcgct tcatagtcgc gggggatagc atccatcttc tcaccccctt
     4981 tctgtggggg acttaggtgg ttctctccct ttccgctaac aagactttga caaggccgcc
     5041 agctacccgc taaatacctc ctttttgaaa atttctcaaa aaattttttt ggggacagcc
     5101 gggccgcaaa agacgacaaa aatcgcccgg caggtcgcaa acctaccggg cgagaaaatg
     5161 ctttgctatg ctgctgattt acatggtata gtacatactc ataaccggaa tagccctgtg
     5221 gaggggcaag gctttttttg agtggtggaa gtggattaga gcatggcaat agcaaaacag
     5281 catagcggca tcctcctatg ggccgccaca ccttttgctg ccagaaaaga acggcggcct
     5341 tgatttctca aaaccgccgc cagcaaaata caaaatgggc gtgggaaagg tctgcccaac
     5401 agcggttttt ttcttttctg ccgctgggca gattgaatac tataatttgc aatgtattac
     5461 aaaagatgct ttaaccactt tccagtaaaa gattgtccat gaccagcata gattttatca
     5521 aatccaaaat ctgatacaaa accaatactt tcattatgaa acggcaaaat taaaataggt
     5581 atcggggtat ggctcatcaa tcaacttata gtgccaccat tcaaagcgat aagactgaaa
     5641 tccgctgctt tccatgattt tacgcaagca tcttcgattt tgtgcctctg catcccctat
     5701 cccttttgct gtatgatgcg accgttcgtc cataaaatcg aagttacttc ccattgaaac
     5761 aagttcccct gtatccaggt ggtagagcgt aagatcaatt gcgcttccac ggctatggct
     5821 ggattgtgag gccacataac cctttgtaat caactcggct cgctctatat tggggtaaaa
     5881 tttttctttt gtgagcttgt cctccggttg cgctgcccaa cgcaggaagc agtccaccgc
     5941 agttcttggc cgatatccat cccataaaag caagccatag ccaagcgcag ccgcatggat
     6001 ttgtgcctcg cgcagagcaa acgccacggc ctttgtgccg atgatgcgat tcacctcata
     6061 cccatccacc ggttttcctg tgaagttatc ccatgtggcg tacttggcat cccaacggac
     6121 gccatgcaac atttcatcta aaaacaaaaa accattttcc atacttatca cctctttaac
     6181 gccaatgtaa tcaggctgtc aatcagtgca ggaagcgtga ttcctgcggc ggccaccata
     6241 cgtgggtagc ggctgtacga tgtaaaaccg ggcatggtat tgacctcatt tagaacgatg
     6301 ccgccatcct cctgcaaaaa aagatcaaca cgggcaagcc ctctgcatcc aagcacccga
     6361 tatactttct ttgccgtttc ctgcacccga tttcgttcct cgaccggaat gtctgcggga
     6421 actgtaatca tcgcattttc tgagcctttt tccggctcgt tttcctgatg gatgcggaag
     6481 ataccgtggc tcagccggat ttgatccact tcgccgacaa tcaaatcatc ctcgttcccc
     6541 atgaccgcac acccgacctc acagcccgaa atcgcttgct caattaagat ttttccatca
     6601 tattgtcctg ccgcttctat cgcagcgtta agttcttccg taccgtttac tttggttacg
     6661 ccaaaggacg aacctgaccg tgccggcttc acaaagacag ggtaggtaag cgcacccgcc
     6721 tccggcttgt cacctttatc aatcatttga aattcgggaa cggcgatgcc cgcatttttt
     6781 gtaagaatgt aggccagtga tttgtccatg caagctgcgg agctttgaat atcacagccc
     6841 acatagggga taccagacaa tacaaacagc ccctgtatcg caccatcctc cccgcatttg
     6901 ccatgcaaaa ccgggaaagc cacatcaata cgccgtgttt cgtattcgct ttctttcatg
     6961 acaagcagcc catgcgtttt cctatccggg gagagtatgg cggggagact gtcggcttcc
     7021 cattccgtac atggcttctt gcatagcttc catacaccgt tttttgtaat tccgatgtag
     7081 tgcggatcga atttttccgt atcaatgttc gcagcaattt ctattgcgga ttttaccgac
     7141 acatcatgtt cctccgagca accgccgaag atgattgcga cttttattct attcatgctg
     7201 taaactcctt tcaaaattga gacaattcct gattgttttt tctgtagtat cctgcagcac
     7261 acgctcagtg tagtacgccg tatggggtgt gatgatcaca ttcggcatcc ttaaaagctg
     7321 cgacaaaaag ggatggtcaa gcactttctg cgagcaatcg gtataaacaa actgatcctc
     7381 gccctccaac acatccagtg ccgcaccacc cagctttccg ctttccaacg cctccaccag
     7441 cgccccggta tcgacaagcg ctccgcgccc ggtgttgatt aaaaatgcgc cttgcttcat
     7501 ctctccaatt tgcttctgac cgatcagatg gcgggtatcc gcacaaagcg gcacatggag
     7561 cgtaacaata tcgctgtttt ttagaagctc atcaagctgg acataatctg catccatttt
     7621 tcggctgtta tcataggcca gcacatggca tccaaatccc cgcagccttt tgacgaccgc
     7681 ttgcccgata tggccggttc caataactcc cacagtcata tcccccagtt ctttcccccg
     7741 gatacgatcc aatctgaaat cttgttgcgc cacggcgtgc atggtgggtt ttgcaccccg
     7801 tatggccatc agcatcagca tcaaagcata atccgcaacg ctgtccggcg aatacgcaac
     7861 cgtgccaacc gagattccca ttctctcggc ggcagtcgta tcaatgtgat cgcagccgat
     7921 gctgcgggta gaaatgtatt ttaccccgac ctttctcagc gcaagaattg tcgcctcgga
     7981 aacctcggac ttatggccta cgctaacgca ttgattgcca gcggccaatt ttgcgttgtc
     8041 tgccgatatc gcatcgctaa tcagcgtagg gataatatga aaatccggcg ataaagcgcg
     8101 gaaagcattt gcctcatcct gttcgcatcc aaaaacagta atgcccacac tttttctcat
     8161 tggttctcct cctgaatatc cacactttcc gggaggggat aacagatttt gcatttgttt
     8221 gtataaagca gcttgcaatc tattatttcc ccggtaccta aggcaacttg catccgttta
     8281 acctgggcat attcataaat cccgccactt tcacggacat actggatact gccatttgta
     8341 attttgtata atgcttgggg ctctttgtcg gcggacacct gaccgatgat tttctcatca
     8401 tctaaggtca cccatatttg gtaggtgcag tcggtatcgt ttcgcacttt taaatcaatc
     8461 cagccctctg aaatggttgc atccacccct ttaatctcgt cactgtttgg ctctggaaac
     8521 tcctttactt cgtgacctct gcgctggata attgtcaatg gcgtatgcag gaacatccaa
     8581 aatagtaaat tgctcatctg gcacataccg ccgcccgaca tggtggtgag tttaccattg
     8641 gtcaccgtaa ggccgtcttt atagggggta tctttgtccg catggcgtac cagccaccag
     8701 aaagaaaagg tttcccccgg tcttatcaat aagccgttta aggtctttgc tgccagcttt
     8761 aagttgaaca ccttgttttc ctggtatatc atgtcaaatc cggtattgtg gttgtataat
     8821 gcgcaatccg tttcaaagag caaatgggaa agcgattttt cccctatcgt ctgcgcatag
     8881 cgacagccgt caaatctcat tcccgcataa aagcacattt ttctctgcgc ccgtctcatt
     8941 ggaagcagga acgggaaacg ctgtgtcaat ctttttctgt ccataacata acatcctttt
     9001 ctcatttttc tggttttaaa tattcctcaa ggcaaacccc ttggttgtac atctctgtgg
     9061 cagcttcgat gccaacatat cggtaatgcc acggctcgtt gctcacaccg gttatctctg
     9121 tcttgtctgg tgggtagcga cgaataaaac caaaacgata gctgttttca tccagccatc
     9181 tataaacctc gttgcctgtt gaatgaattc cgtccgcatt gatatccaca gcaagaccaa
     9241 gctgatgctc gctcgttccc ggcacggcca cccaagtttc tgcttccgct ttagcctgtg
     9301 cagaggtgta gcctttcgcc ttatattcgg caattttttc atccataatt tcttgctgtt
     9361 tttctgttgt tcggtatccg gatgcgacaa tcgggtaaac tccatcagtt cttgcggcat
     9421 caaacaaatc ttgaagatag ggagaaatcc gaatatctat ccgctcacca tttgatagtt
     9481 gctcaagctc cacatcgtac tgtgcgggga tggggttctg cctgttcact aaaatcaggc
     9541 tccattcctg ttcatctccc tgttctttgg cggtggcaag agcgttttcc tccaaattct
     9601 ccactacgtc agtgctgctg cctgtatgag ttgcagggat gggcggtatc tgtgttgcct
     9661 ccaatacgga agccagtctc caccccaaaa acagcgtgca gactgtgaac gaaataatga
     9721 acgcacatag aaaagcccgc ttttccacgg attgtttcat atgccgtttg tgatgatccg
     9781 cattggaatg atagttgctt ttttccattt gatcgaatgc ctcctttgca ataaaactac
     9841 gatttgtggc tggtacgatt gttattatgg caagcgcatc tgtactttcc ctgtagccag
     9901 tctgtagttt tcctgtagca gcaaaaaaag accggcaggg ttatacctgt cggtcaaaat
     9961 catttaagtt ttaaatattt acaatgttga tgtgagcggt aaatccagcc agaacaaaac
    10021 gccatccgag gtgttttcca gcgcatactg gaggctcatg gcatccagcg ttttttgtac
    10081 gatggcaagt cctaaaccgc ttcgcccact ttttctgctt cgcgcctgat caatgcgata
    10141 gaatggggtg aacagccttg gaagcgcagt atcatcaatg tgaacgccca tgttcaaaac
    10201 aaaaaggcgg catttttcag caccaggctc actccatatc cgtacctcgc ctccctgcgg
    10261 cgtgttctga actgcattca atatgacatt ggatagtgcc ttttggagca gcctcggatc
    10321 ggacaggaca atttgcccgg ctggaatatc tgtgacgaac cgctggttgt ttgcctctgc
    10381 caaggtttga aaatcgggca gcaactcggc aaccgtgcgc cctatgtcca acggttcagc
    10441 tatgggtacg attctcccat cattcaggct gacaagctcc agtatttcgg aaatgatttt
    10501 gccctgccta tccatcattt tgatgcattc gcgcagatac ttagaatgat ctttgtagtc
    10561 accgatattt tcaagcattc cctccaacag aacgcttgta gccgcgatgg gcgtttttag
    10621 ctcatgagaa gctgccgcaa agaaatatcg ctgtgtttcc tccaactcat gttcccttgc
    10681 gatttcatcc tccagccttg cgatggtttc tttcagcctg acatacatgg aatgcatatc
    10741 atgagccagt gcgccaagct catcctttcg ctccagcggc ggcggtacat ctttcaggtt
    10801 tgccatttta ttcgcactgt ccgctaaggc tttgatcggc gttgtcattt gccgcgcaaa
    10861 gatatacgcg cataaaaggc tgaatacaac cattatcgca atcataacaa ttccccgaat
    10921 tgtcagccct tgataaagca atcccacacc tgccttgctt tgagcaacaa tcgaaatatt
    10981 atcatctcta tgtaccacat aaagaaagtc gggtctaaaa ctatttgatg tattggcatt
    11041 tggtgtggca tagagtacgc ttccctcttt atcttcaata taaaactcaa aggattggtt
    11101 attgtagtga aacagccctg ccacctcttg aatatcaagc ctatcgctat tctgaatcag
    11161 ttccaccaat ggctgatagg attttactgt ttgctgtaat tccatcactc tgaaataaga
    11221 cacaaattgc tgtgcaaaca gtgttgctgt tacaccgaca agcagtagca ggacaatgat
    11281 cgtataggaa aaaaccttaa tgaaaatccc ttttctttcc attattatga ttcctccaat
    11341 cggtatccta caccgcgaat ggttttgatg atattttccg gcagcttggc ccgcaaattt
    11401 ttgatatgag tgtggactgt gctgccatca ccctcaaagt catatcccca tatacgtgac
    11461 aaaatgattt catgcgttaa ggttctgcct ttgttctgca ccagcagcaa aaggatttca
    11521 aattcttttc gtgtcagggg cagctctgta ccgtcacaaa gtaccgtgaa atcctccggc
    11581 agaagtgtca gcctgccgac acggatttcc tttgccagcg caccgctgcg ccgcagcagg
    11641 gcttccaccc gctttagtaa aatctgcatc ttgaatggct ttgttacata gtcgtctgcc
    11701 tctgcatcaa acgcccggat ttggttttcg tcatccgaca gggctgtcat catcagaatg
    11761 ggagtatcat ttttcgcacg aaattcacgc aaaagttcat gcccgttcat accgggcagc
    11821 ataatatcaa gaataaccag ttgataagtg ttttcgtaaa acttggtgta tgcctcattt
    11881 ccatctgtgc aggcatccac ctgatatcct gcctcagcca gaaacgccct tactgtatta
    11941 cagatatgat catcatcctc gacaagtaga attcgtatcg acataaaaat ccctccattt
    12001 taagaagata acataacagt ctgtagtttc cctgtagttt caaagcgatt tcttttcgga
    12061 ttttaatcat ccgtcattgg gatactcgct tcttttcatg gtgtatgtat ctatattatt
    12121 ggtttaaacg aattaaatca agataataat aattaacacc ttcttaatgt ttgggatgtt
    12181 tcgattacag cggttgtcaa aacatcccga taatactggc aagcaatgga tcagaatatc
    12241 ctttacggat attctgatcc accacttgcc cgcctgcggc gttctcgttt tgcttgttgg
    12301 ggacacccca agcctcagca aaatgcgcgg cctctttttt gcgaatgcac cttgcggtat
    12361 ggcttttcac acaggcaggg cctgtgtgta ctccctccgg tcgctcttgc cgcactccgc
    12421 gcagcagatt cagtcctaat accgttcaag acagtagaaa atcagcaagt ctatattcct
    12481 catatcttca tcatccgtgt ccttgcagtt acaggaaatg taataatcgc caaatgtaaa
    12541 tcccagaata ttttcctcaa aaagccgtac ctccccggta ttaaaatcaa tcaccccata
    12601 ctcaaaggcg cattagacca gcagttatcc aatgtctgga gacttcacca taaagaatcc
    12661 agatgccggc cttttcaatt ctaccatata cggctgatac agcagcagct tcctcgtctt
    12721 tgtttcgggc attttccatt tctgtcatga taaagtttcg cttcttcata catccatgtg
    12781 taaatctttt ctttctgttt cagatgagaa tactttttat cggtctggag cagcctgcca
    12841 tctatcatct tatagttttt ccgtgacaaa ataccatgct ttctctctct tcaaaaccat
    12901 atccaaaatg cgccggtttc attatactat tcagtaaccg cctttccttt ctttgtacgc
    12961 ctgtcaatcg gcttttccgc agttttggga atgagccaga tcgtcgccat ctttacagcg
    13021 ccggggatgc gtcccgcagc acaatagtag tttatccatc gtgggggcac accccatttc
    13081 tcgcctgcct ctttcaacgt catgtagtcc atagtacacc tccacagggt acattataat
    13141 tcttttgctc gaagaataaa agaaacaaaa tgtgaaatat aaactttaag aaaggtgcat
    13201 ataccaacat tccacgggca gagtatgaaa tatacaatgt aagtgggagg tgcgatatgg
    13261 cgaatattga agattgtccc ggctttgaaa ctttcggcgc ggatgtgaaa gcggcccggc
    13321 aagcaaagcg cgtttcgcgc aaagcaatgg cagagaaaat aaatattgac tggcgctatc
    13381 ttgccaacat cgaaaacgac ggggccattc ccagtctgcc ggttgtcata cagcttatta
    13441 aagcctgcgg gcttcctgtg gaacgctact ttaacccgga gatcatgcgg gaggaaagcg
    13501 aactgcggca aagggtcagc cacaagctga aactctgccc cgaagaatac ctgccgatca
    13561 tcgagggcgc gattgacagc gccatactaa ttgaaaagcc gaagaagaat gaaacggagg
    13621 atgtgtaact ggcatcctct gttttacttt tagcgggagc tgcacagcgt agcttctatt
    13681 tttttgcact tttttctttc cagagggcta ttttgcgggt agtttggggc tttggaaacg
    13741 tcttgttagt ggagaaacag taacgtagca agcataggga gtgtagccac tcccggaaaa
    13801 acggcctgcc ccgtccctgc cgggccgcgt ccgttttgct tgttggggaa atccccaaac
    13861 ccctgaatgg aaaggacggt gaagcatggc aaacagaaag cgaaaaatcg tgctgcgcgt
    13921 ccctgtgacg ccggaggaac gggctatgat tgaacagaaa atggcgctgc tgcatacgaa
    13981 gaacttttca gcctacgccc ggaagatgct cattgacggc tatatcgtca acatggacac
    14041 cagcgacatc cgggcgcaga ccgccgagat acagaagatc ggcgtcaacg tcaatcagat
    14101 tgcaaagagg ctgaacggta tggggccggt gtacgcacag gacatcgagg atataaaggg
    14161 ggcgctggcg cagatatggc agttacaaag atacatccta tcaagtcaac gctgaaaaaa
    14221 gcccttgact acatcgagaa cccggacaaa actgacggca agctgctggt ggcctccttt
    14281 gcctgctctt atgaaacagc ggacattgaa tttgaaatgc tgctggcgca ggcataccaa
    14341 aagggcaata atctgggcca ccacctgata caatcctttg cccccggcga agccacgccg
    14401 gaacaggccc atgagatcgg gaagcagctt gccgaggaag tgctgcaagg gaaatatccc
    14461 tatgtgctga ctacccatat tgataagggc cacgtccaca atcacattat tttctgtgcc
    14521 gtggatatgg taaatcagcg caagtacatt tccaacaaac agagctatgc ctatatccgg
    14581 cgcaccagcg acaggctgtg caaggaaaac ggattgtccg tggtgaagcc cggccagagt
    14641 aagggcaaga gctatgccga gtgggacgcc cagcggaaag ggacgagctg gaaagcgaag
    14701 ctcaaagcag ccattgacgc cgctatcccg caggcaaaag attttgatga tttcctgcgg
    14761 ctcatgcagg cgcagggcta tgagatcaag ccgggaaagt ttatttcttt ccgcgctccc
    14821 ggacaggaac gctttacccg ctgcaagaca ttgggggaag cctacaccga ggaagccatc
    14881 aaagagcgta tcaaaggccg cgttatcacc agagcgccca aagagcgcaa ggggatttct
    14941 ttgcggattg atctggaaaa caacattaag gcccagcagt cggcaggcta tgagaggtgg
    15001 gcgaagctcc acaatttgaa gcaggccgca aagactttga atttcctgac tgaacatggg
    15061 atagacacct acccggattt ggaaagcaaa gtggccgaga tcaccgccgc cagcgacgag
    15121 gccgccgcta ccctcaaagc tatggaacac cggcttgcgg atatggcggt gctgataaag
    15181 aatatttcta cctacaagca gctctggccc gtggctatgg aataccgcaa tgcagcggat
    15241 aaggcaaaat tccggcgtga gcatgaaagc acgttgattt tgtatgaagc tgcggcaaag
    15301 gccctaaagg gacagggtgt gaaaaagctc cccgatctgt acgccctgaa agccgagtac
    15361 aagcgattgg ccgaggaaaa ggaacggctg tatgagaagt acggcgaagc gaaaaagcag
    15421 atgcaggaat acggcatcat caagcagaat gtggacggca ttttgcgtat gacgccggga
    15481 aaggaatgga cgcaagagct atgagtattc cacgaatgaa ttaccctcaa tatcgcaagg
    15541 cccggaagct gacgcatgag tgctgcaact attgtgacgg caactgcctg ttgctggacg
    15601 acggcgaaga atgtatttgc gtacagagca tttcctattc gcttctgtgc cgctggttta
    15661 aggccgctgt cctcccgctg gatgcggccc tgtatgccga gatcaccaaa gaccgggacg
    15721 aagtgaaacg ctgctgtgag tgcggggcca cgttcacgcc gaagtctaac cgggccaaat
    15781 actgcgcggc ctgctccaaa cggatgcgcc gccgcaaaga ggccgagcgc cagcggaaaa
    15841 gatactggaa acctacgcac ttagagctgt aaaagccctt atatttcaag gcttttccgg
    15901 catggtcagc ggggtatgtg ggtgttttat cctatccccg ccgaagtgct gttctaaatg
    15961 cgtagaattt accagcagaa aagcgccgtt ttctctggtt ccatgcttcc gaatatggta
    16021 tcatagaaaa aatcagtctg acgggggtat caggatggac ggacaagaaa agctgctgga
    16081 ctatgaaacg atcaaagccg ccgttgccgg tgagaggtgg gccacggaaa aagtgttggc
    16141 ccattatgct gactacatca acgagctttc cacggtggag atcagacagg caaacggcaa
    16201 aaagaagaaa gtcattgacg aagatatacg gcagcgcatt tccctgaaac tgctggaagc
    16261 attgccgagc ttcccgctgg aacagagata gacggcaaga gggctgcacc ctcttacgcc
    16321 gctttgcggc tatcacctgc acacccctga aacagataca cccgctgcgc ggctatatct
    16381 gtttcagggg tctaattata gctgcaacct acgcatttaa gggattgaaa accgcccgct
    16441 gcaaggcttc tcccgcatgt ttgcaggggc aagcgaatac ttcatcctat ccccgacaat
    16501 accgggttct aaatgcgtag gaataggcaa aaagaaaaga cgccggacgc aagcgaccac
    16561 atggccgccg cgcccggcgt ctgctgttcg ttattcgtct gtgaagtgga acttctggcc
    16621 gtcatagtcg gcatccgtca tgcgttccag cttggaaagg gtctgctggg cctgcgcccg
    16681 catatcccca tccataaccg gcatgacggc ggtgagcctg tttatcgttc tgcgccggtc
    16741 tgcattgtgg tacatacaga tcaggttttc ttcctccacg gtaaagtgca tcattcctta
    16801 tacctccaat tcattctttt tggttctggc cgccgccttt tttgggctgg cggcctgttt
    16861 gctgtcggat agctgcttgc ggatagaggg ccggggcctg cgaatactca catcccggcg
    16921 ctcgtcccgg ttaatcacgg catacacgcc atgcctgcct ttcatcccgg taaaacacag
    16981 ggatttatag ggcagcatgg caaagagccg gtcattatcc ttggtggaag caatcgccgc
    17041 aaagtgcggg gagatctcca ccatgaaatg cgttttattg gggctgttag gcgcggacag
    17101 atctttcagg ctgtccacaa tacgccgggc ctcggtttca atccggctgt catgcaaagc
    17161 ggtgacgtgg actttgaaat cgccggggac ggcccgcccc cggctgtgct gctccgtccg
    17221 tagcgcctcc tgcagcacag ccgggtcatg gggctgggcc tcatagtaca aaaagtttcc
    17281 caatacccgg tcagggcttc cgtcaaaacg ctggctctcc gggatgtcac gggtgccatg
    17341 ctcataaaag agccggttca cactcaccgg ctgggcggtt tcaatgacgt gccggaaatg
    17401 ctgcggatag tccagctcat agagattgcc ccggatagtg ccgccctgtg tgccggtcag
    17461 ctccaccgcg taggccagta tcttgtcacg ggtctgctcc ccgtaaaatt tccatgtgtt
    17521 gtactgtcgg gtgtctttca ggaaaacatc acgttcccgg aaacagtacg tcccggaggg
    17581 ccgggacatc cagagcaggg tcttgtctgc ggcctgatcg ctggcggcat cccgcctgat
    17641 aatgtctttg tcaatgccaa agtcactttg ataaaacgcg gtgttctgcc gcatgatctg
    17701 ttccagcgcg gccagaacgt ccacattttc aaacttctgc atttactcta cctccaatcc
    17761 ctttgatttt tcccgcgccg gtggctgctt ggcctgttcg gtcttggccg cgttgagctg
    17821 cgcccggata gagggctttt ctttctccct gcgggccgca agctgtaagg cgctgggctt
    17881 ttcgccggaa aggggctgga tgcacttgat acggtcagcg acgcggattg tggtgtcgtt
    17941 taactgccgc tgccatgccc cggccttggg cgaccagcgg aaaccgttgc ttttcagctc
    18001 gtcgcgtaca gcagcatccg gcttcccctc aaagaagatt tgcaggcggt tggccacccg
    18061 gttgatctcc acagtgccgc cgtcaaactc ccatcccaca taggccgcct ccttttggcg
    18121 ggtcaattcc tcaatgcggc cctttaaccg gcgtatctcg gcgttattgt tggagagctg
    18181 ccaggactgg aacggttttt tctcatagtg ccagcccgac gccatatccg ctttcaggtt
    18241 ttcaatgcct ctttcggtca gatgggggca tccgtccagc gtcccatgct tgcggtagta
    18301 ggcattgacg gccttcatca actcctgcga cgcttccagc ttttccagtt tcttttccag
    18361 cttggaaacg gcctgcggat cgtcggcgct gataccaccc atgccggtgc tgcggatttt
    18421 gtcaagcaag ccctgaatgt actgaaattc ctccatgttg cggtcactgg cggcgttctg
    18481 cttttctttc gcccgtaccg ggaaattgga cggcccggca atcaggacag aggggacgcg
    18541 ggcggtaatc tcatagccct tattcatgtt ctcggccagc ttgcgggcgt aggtgtcaag
    18601 gaggctgtct atcttttcgt gatactctgg ctccacacgc tttttctggc gctccgcaat
    18661 ttccacggcc tgatcgacat agtgccggta ttccgccgtg gcgctgccgg gccggtagtc
    18721 agaaaagctc atggcttcct tggcgcggcg ggcggctccc tcattgatgg gataatagcg
    18781 gacagccgtt tgcgggggcg cagcctccgg gggttcctcc tgtgtctggc cggaagcaac
    18841 tgccacatcc tcaaaagata gctgttcggg ttcctccggc tgcggttcgg gcggcgtatt
    18901 gttgataatg ccgtcaatca tgttttcgtt ctgctccgta gacagttcgg cggtacgcag
    18961 gggattttcc cgcaaaaact ccggcacttc catatagccg aagctgtcta catagtgggc
    19021 ggtgtcctgt ccattctggt gcagcaccac cacgtcggaa acggaaaggc tgtgtcccct
    19081 aaaatctgcc gggtggtcga tgttgaatgt gcggtaaata tcctccaaag acgtgctttt
    19141 atccagcggt gccgtataaa caaggtcata gttggcactg tccacggcca gccctgcggc
    19201 ctgtaaacgc tcgtatggct caaaccgata gtcgcgggtt tcctccccgc ctcgaagctg
    19261 gtagatggaa aaggtgtcct gttggggtgc ttcatagcca gccgccgcgt attcctccac
    19321 ggtcataccg gctgcggcgg cctcctgcgc gatctcggcc tgtacctgtg ctttgtagtc
    19381 ctgcaagctc tggtcgaagt cagccagttt cggcggctcc ggcaactgat actggccctg
    19441 attgagcatg gggcggcact cctgcacata agagagaagc gcccccaaat aggccgtttc
    19501 ctccggcgtc agggtttccc cggactgtaa gcggccctgc gccgtctgtt ccatttgggc
    19561 aaggctgcaa tgcagcttca tataggggac aaactcgcct aacagcatat cccgatgggc
    19621 cttgtcctgc tcccagccct ctatgccggt tgtagcgatt acatgctttt tccagttttc
    19681 gtcctgcgcg taaaattggt ggtagctctg gatatgttcg atcagggaac cgtccctatc
    19741 tccaaaatcc tgccgcccct cgtagctgtc cggctcgccc tgaaagatac agtcaatgcg
    19801 gaacgcggtt ttgtcatagc ctgcctctgc cggatatgct gcatccagac gggcaaacag
    19861 cgcgtcggcc tcatgcaacg gcatttgctg gccgtccttt agctgggggc tttcgctcca
    19921 tagcacagtc acaagcggct cggctgccgg atcaagggcc ggggcctgtt cttcctcggc
    19981 ctgcgccggg gccgctgcct ccggggtaac gtcaaccgtg acggcctcct gttcctgccg
    20041 ggcctcgcgc tcctgctgaa gctgggcaaa atggccgtcg atctcggtta tcaattcgct
    20101 ggccgtggcc cggatggttt caagggaggc tttcagctcg gataactccc tgccgctgct
    20161 ccaatcggcc acatagccaa aagaatagtc cgacgtgtcc agcccgtaat actggcagac
    20221 ggtataggcc acgctttcgg cctgtacctc gcgggtgctg cggtctggcc gggccgcctg
    20281 ttcctcggcg ggggcctccc ggtcaatggc gtggagcttg gcatgggcaa tctcatggat
    20341 ggcggtcttt agggtctgca attcgctcat gccctcgttg atggcaatgc gctgttttgc
    20401 gtagaagcag cggcccttgg ccccgtctga aatcggctca atgcttatgg gatagggtga
    20461 tgtccgttcc agcgcggcaa agaaatcctg atactgctgc acgtcgccgg taagttcact
    20521 gatatagggc atgggaaaat ccttgccctc ggtctggctg atgtcaaaga ctgtcaccac
    20581 cttaaaggcc ggaaccgtca cttcctgttc ttcggtcacg ggcttgccgt ccgcgccgat
    20641 cacgggctgc tgggtggccg ggtcgatttt ctccatctgc tgcttaacct tgaacggggc
    20701 cggggccagt atcttaatgc ctttctcgcc ccgcttcaca tggcggtcaa attcatcctg
    20761 ccacctgtta tagcccgcca caatcgaacc gccctgcatg gcgatcagca gcgtattgtt
    20821 cagactgtaa tcgtgcatct tggacatagc ggataagtaa gccatatagc ggctactttc
    20881 aaaaagctcc tgtacgccgg tttccagacg gtcagtaatc actttcatgc gttctttgct
    20941 gttcatgccg tccagcatga tcgggataac cgggggcggc gctttgtcgg ggctttcgga
    21001 tgcctccggc tgctgggcct gcggttcctc ccggtcagct tccggctggg ggtagtgcat
    21061 cacccggtat tcctccggca ccggctggcc ctcatggacg cgctgccatt cgtcgccgct
    21121 tctcaccaga tagccgtact gggtaaaaac gccttgttcc tgctgggcaa tgtggcgtcc
    21181 aaactgcgcc gtatcaatcc cggctttcca ttcctccggc atatccacca tgccggaacg
    21241 gttcaggtag tagtcgccca actgtccatc ccccctaacg tcgggaagat gcacatagta
    21301 atccacattt tcggtgtagt cgatgatctg gccgatattc tcaaagccgc cacggggatt
    21361 ttgcagggcg gcgttcagct ctgcgatctc ggcggcgtca agctggccca gccgggcggc
    21421 aaggaaattc agctcgtcca cgtcggcggc cagcaccatg tcatagggaa tggcaaggtg
    21481 tctgtcctcc gggcaggaat acccgttgat aaaaaaatcc tgcggattgt cggcagtaat
    21541 gccaacatcc cgcagggcgg cttgcagcgt ttcggttgtg gtgggcaaat ccagccagac
    21601 gccttgtttg ccctgttcca aaaactgccg gttatggact tggatagaaa atacttcgtt
    21661 cacgcggtca ctcctttctc tgttctgttt caaaaaatag ggggttgggg acttccccaa
    21721 caagcaaaaa tccccggccc ggcagggacg cggccggggg attttaccgg gagtgtagcc
    21781 acactcccta tgcttgctgt gttactgtcc tttagctcct gtgttcaatg cggtagttga
    21841 cgtacttgcc cccggtatca gccaaaagca ccgtcccgtc gtaggttttg ccggtcttta
    21901 cggaatagag gccctttacc ttgactttgc cggttttgag cagcgcagcg gcgatcttcg
    21961 gggtgaacgc tttcttgcgt tcctcaaaaa atcggtcatt cttccacatg acgaactggc
    22021 aggcccggtt tccgcagtag tagtttttct tgccctcata gacagcctca ccacagcggg
    22081 gacacgtccc gataggaaca cgctcggcct gaaacaattt ctgcttttcc tcactgatac
    22141 aggaataggt ctgcaccagc tcccgcgcct gcgtctcgat gccgtccata aattcgtcag
    22201 gatcggcctg ccccttggcg atctcggtca gccggttttc ccattcggca gtaagctggg
    22261 gagaagtaag cgcctccggc agcaccacag ccagattgat gccgtccttg gtgggaatga
    22321 gctgcttgcc ttttctctgg acaaagccca tctgcaccag cttttccaga atggcggcgc
    22381 gggtggccgg tgtgcctaat cccttgcgct cggcatcctc cggcatatcc tcggccccgg
    22441 cccgttccat agcggacaaa agcgtatctt cggtgtaggg cttgggcggc gtggtgaaat
    22501 gctcggtgac agaggcggcc acgtctgcaa aggtctggcc ctcgcaaagc tccggcagct
    22561 cccgcaccgc ctcggcgtcc tcgtcggcgt cggtctttaa ggaagaacgg aaccggcgtt
    22621 caatctcttt ccagccgggg gaaaggacgg tcttgccctt ggcggtaaac tcctgcccgg
    22681 cacaggtaaa tgtggcggtg acggcctcat agatatgcgg cccggccacg gcacataaga
    22741 gcttgcagca gatcagggac agcagctttt tttcactctc ggccagagtg ccaaagccct
    22801 gcttcacaaa ttcagcggtg gggataatgg cgtggtggtc ggacaccttt ttgctgttca
    22861 ggatgcggtc aaactgcggg gaaaggttca ggcccttggc aaacggcacc agctcccaca
    22921 ggccggacac aaggctttcc acggcgggca gcatatcgtc ggtaagatat tgactatccg
    22981 ttctcggata ggtcagcagc ttctttagat acagtgcctg ggcatagtca agggtctgct
    23041 tggcggtata gccgaaaagc cggttagcct cccgctgcaa ggtggtaagg tcatagagct
    23101 tgggcggctg ttccgtcttt ttctcccgcg tcacggaaac gcaaacggcc tgcgcttttg
    23161 cacaagccgc cttgacggtg tttgcctcgg cagcgtcgga aaagcggctg cttaccgcgt
    23221 ccgcgccgcc cgccgtgata tggacgatgt gatatttctc tttcttgaaa tcggtaatct
    23281 tggcgtccct gtccaccagc atttttaagg tcggcgtctg gacgcggccc acagtcaggt
    23341 tcttgtggta caggacggaa aaaagccggg tggcgttaat gccgatcagc cagtcggcct
    23401 tggcccggca cagggccgat tgatacagcg gatcatagtc gccgcccggt ttcaggtggg
    23461 caaagccgtc cttaatagca ctgtcctcca tagaggaaat ccaaaggcgc tcaaagggct
    23521 tttggcatcc cgccatctgg tagacgaaac ggaaaatcaa ttccccctcg cgcccggcgt
    23581 cggtggcgca cacaaggccg gtcacgtcgg ggcgctgcat cagcgaacag agaacgtcaa
    23641 actgctgttt cttgtcgtcg gggacgatgt actgccatgt ttccgggaga atgggcaaat
    23701 cctcatagcg ccattttttg aaacgctcgt catagcgtcc ggcgtcggca aaggacacca
    23761 gatgtccgat gcaccagctc accagatagc cgttcccctc caaatagccg tcgtgtttct
    23821 ccgtggcccc gatcacgccc gcaatgctgc gggccacgct gggtttttcc gcgattacaa
    23881 gtttatatgt cattcctcgt cctcctgttc actcacaaaa tcagcttccg ggtcaaactc
    23941 ggtttcgggt tcctcgtcct ccaaatcctc gtcctcccca aaatcatact cgcccaaatc
    24001 gtcgctgccg gtggtttcag ccttgggctt tttgaacttg aaatagtaaa gggccgcacc
    24061 gccgcccgcc agcgccacca gcaggaacac gatcaggccg cccgcgccgc ccttgggttc
    24121 ttcctccggg gcttcgggtt cctccggctg gggttcctgc ggctccgggc cgctgcactc
    24181 ggtcaggttg gttttacata ccgggcaatt cgtgttcaca gccccggcct tgcatttcgt
    24241 ggtacaggag cagacagcag cgggggtgac ggtttccccg tcctccatca gcgtcagcag
    24301 atcggcttca tccacctgat ttaagaaatg gacagtttct tttccctcgt cgtcccggtc
    24361 aataatcaga taaaacacat tgccgttttt ggtttccacg gtgataaact gcttgccatt
    24421 tttcgcggcg ctgccgatgt cgtcaatcag tgacaggttc ccgtccgggg acagggcggc
    24481 gctggccccg ccgccattga acagttccag cagagaagca atttcctccg ggtcaatgga
    24541 gagggaaaaa ccgccctcgc cggtagccgc tacttccttt tcgctgacgg tcatatagtc
    24601 ggaatggaca tagccgatgc gctccggcaa gagaatttta atccagtcgc cgtctgtgcc
    24661 gactacctca accgtcgtcc cattgggaag ctgggtgaac gcggtcttgt caagccccgc
    24721 gccggtgcgg acattcagcc tgccgccgtt ggtggtgacg gtgccggtct gttcaggttc
    24781 ttttttgtcg gtgccggtgg tgatctggat gccgtcgtcc gtggcggcaa tgctcacctt
    24841 ggaaccgaac agcgcggaaa acaggtcggt gatgtccttg ccggtcagag ggccggtatc
    24901 tttttcggcc tcggtttctg tcccgtccgt ggcggtgccg gtttcgtctg tttcaggctc
    24961 caaaccgccc gtagcgggtt cggttgtagc ctccggcagc ggggccggtg ccgggtcatt
    25021 gccgcctgca agggcggtaa cgggaaaggc cgtgacgcag agcatcacgg ccagaagtgc
    25081 ggggagaata cgggaaatcc gtctggtctt attcttcatg ggcattgtcc tcctgttcat
    25141 ggtaaggggt tgcggcaggg gcggcctgca ttgcgccgct gcgtaagaac gcggcaaatt
    25201 cctccggctt catgttcatg ccgcgcacaa gctggatgat ctgcaaattc tcctgttcgg
    25261 ttttctgcgt ttccagctcc cgaagctgtt tctgaaactc cgcgatctta gacttggttt
    25321 tctggatgtc cttttcgatg cggtcaagtt tggttgccat aattgaaagc tcctttctgg
    25381 cgttaaggta aacgcccgta actgtaaaaa tgggattgcc agtagtttga agtaaggttc
    25441 gtgtaggaaa tcgggttccc gcaatgcagc atcacattgt tgcccacata caggccgacg
    25501 tgggacacgc cgggggtgtc gtaggttccc ttgaaaaata caaggtcgcc gggcttggcc
    25561 tgcgaactgg aaacaggggt gcagatgtta tataggccct gcgcccccag ccgtcccacg
    25621 ttccatccac tatgattgat tacccacgaa atgaagccgc tgcaatcaaa gctggtgctg
    25681 ggagagctgc cgccccacac atagggataa ccgatatatt tttccgcctc tttgagcatg
    25741 gcggcaaagg tggcatcctc caaagcctcc gggggtacgt cgtagtaggt cggcggcgtc
    25801 agggtggagg cgttggggta ctggctgccg gaaaacagat caggccggtt tcctagcgtg
    25861 gacatataca gggaatagaa acttagcgtt tcctcgtcca tgatatagac tggcacatgg
    25921 gacaggtcga agttttccag cgtgacgtag cagatgtaat agtcatacgg cacttggtag
    25981 gtgtctgtat gggtgttgcc ctccgcgtcc gtccatgtat cggtttcggt gcggtaacgg
    26041 gtttctacca cgacgttttc tgtcaggata tattgcttgt caaagagcat ggacaaatca
    26101 ccctgtacct ccccgatggt aaatatcccg tcatggagcg ccgacagaat ggaaatcagc
    26161 acataggggt catggctgat ctcgtcaagg tcaaaatggt attcgtcata gtcatgggtg
    26221 gcctcgtagt tgtccagata gtcttgcagc tcggcttcca gctccgcgta ggcgtcctcg
    26281 gcggccaaca tatcgctatc ctcggaaaag taagaggacg ccgtgatgcc gcttccggcg
    26341 ctgccgaaca tggcggtgca ggactgtaag ccgccgatga taagcagcat caaaagcccc
    26401 aacacaagga cggccagcgc ccctttccag tggcgggcca caaaggaggc cgcccgttca
    26461 ctttctttgg ctgcggcctt ggccgctttc gtggaggccc cggctgtctt ggccgcgccc
    26521 tgtgccgccg cgcctgcggt ctggcccgct gtccgggcgg ccttggcgta ctggcgcttg
    26581 atctgctgtt tctgccagaa ccgggaaatg gggttgctgg ccgcctgtgc gatctccggg
    26641 ttatcccgca aagatttctg gtagaaatac tccgcgttgg cagacaatag cctgcgctcg
    26701 gccttttccg cgtcccagta gggtttcagc ctgtggtgcc ggatggtgct gcggatggct
    26761 tttccggcct gccgctcggc aagttcctcc cccttatggc cgccctccac acccacgttt
    26821 tcatgctcga cttcatggat tttcccgtgg ccgaatagaa aaacctcgga aacaggacgg
    26881 cctgccggat tgggtttcag ggagggaggc cccttgtcct gtttgtcaaa gtgcaggacg
    26941 gtctttgctt tgccgcttgc ctcgtcaaag acggtttcct tgaccgggac gcgcttcttg
    27001 gggatggcgg cgcgggcctt atccagctta tcggcccgtt tgtccgattt tcggatatag
    27061 ggctgtaaat ccgggtcggc ccgttcttcc tccgacagtt ggagccgggc ggatggccgg
    27121 tgcagggcag cgtccccctc ggcggccaca gccccggcct gcttcgcggc tttcttggcc
    27181 ttatggccgt catggatggc gtcggcccgt tccagcagct tatcggcggt gccgcccggc
    27241 cccggcgcgg acaaatcctg ttcggcctcc cggctggaaa tgttctccac ctcgccggtg
    27301 gcctgatttt ccagcgtcag cccgtcgcgg gtcatggcct gcgtcacttt cgccttggct
    27361 ttcagctctt tcatggtggt tcacctcctt tgtggcgtcg ccgggtcata ctcccgccac
    27421 ctcgtttaag cgggtcgtca taatcttgta aagctgggtg ttgtgcggga tggggttggt
    27481 aaagggcagc accacgtttt caaagatcaa aaggccctcg ccgggttcgg aattgtcgat
    27541 gtacttctgc tgttcggtgg acaaattcaa gcgttccgca aggatttttc tatccccgga
    27601 cgcctgattg agcagcgtaa tgaagtcgct gttttcaagg atattttcaa tttccgggga
    27661 ggaaagcagg tctttgacgt tctgggtggc acctgttgga atgccgcccc atttcctaaa
    27721 ccgcttccaa atctccgcag aataggccgc cgtctgttcc tctttcaaaa gcaaatggaa
    27781 ctcgtcggca aaataccatg tggcctttcc ttggcttcgg ttggccgtga cgcgccccca
    27841 tatctggtct tggacaatga gcatccccag ctttttgagc tgcttgccca gctccttaat
    27901 gtcaaaggac acaagccggt tgccgatgtc cacgttggta cggtggttaa atacgttcag
    27961 ggagccggac acatacaggt caagggcctg cgccacccgg tcagcctccg ggacatgctg
    28021 atctaacaac gctttgtgga ggtcggacag gatcggcata ttctccgggc gggggtctgc
    28081 cagatagggc cggtagatca cgcgcacagc ccggtctatc acggtctttt caatggcttc
    28141 cagcccgttc ttgccgccca tgacaagctc acagaacgac agcacaaagt cggatttcag
    28201 ggccagcggg ttatcgtcct ccgaataatt caggttgatg tccagcgggt tcacaaagtc
    28261 cttgctggta ggcgacagcc ggattacctg tccgtggagc cgtttgacaa gggggtaata
    28321 ttcggcctcc gggtctgaaa taaaaatatc gtcaggcgtc gttaagaaca cactgacgat
    28381 ctcggattta cagctcatgg atttgccgct gccgggggtg ccgagcttca aggcattggg
    28441 gcaacgggcc tgcttcctgt ccagcataat catgttgcgg gacttggcgt tgatgccgta
    28501 atacatggcc gcgccgccct gaaaaagctc ctgcgtcaca aaggggacga acacggccac
    28561 gctggaagtc gtaaggctgc gctgtatctt gatctgattg acgcccagcg gcaggctgct
    28621 tgcaaggccc tgttcctgct ggtagtccag ccggacaagg ggacagttat gcttttgggc
    28681 cacgcctgcg gcttggaaca cgtcgttatt cagcttttgc ttggtgtcgg ccacgttgag
    28741 gacaaggaac gtcaactgga acagccgttc attgcgggtc tgcaactttg tcagcagctt
    28801 cttggcgtcc tcgccgtagg tggcaaggtc gctgggaaga atgtccatat cgtaaccgct
    28861 gcggacggcc tttttctgct ccgcaatctt catagcgtca aggtcggtaa ttttgcgctt
    28921 gatggtcttg atcgccttgg tctgttcaat ggcctgcaca tggagattga ctaaaacacc
    28981 gctttccgta ttgaggaagt cggccagcat atcgtccgac agctccgggg acaggatttg
    29041 caggaaactt accgcgccgt atttgccgcc catgccgaat gtctttgcat tgccgaagca
    29101 cagggaggac ggggcaataa aatccttggt ggagaggccg gacggggcca gccacttcca
    29161 gtcaaaattg aatatctcgc cgtccgggtg gtacacgcca tgcagcaggt tcagccgttc
    29221 ccagccgtcc atcacatggg ctacggcccc catgctcttg aaatagccta acaggtcaaa
    29281 gccgatgcgc ttcaatctgg ctcgcgccgc tttcacgctt tcggcctcga tggtgaatgt
    29341 caggtatttg gtctttacca tgccgttgtt gccgtttgca agctgctttt gcagaatgcc
    29401 ggtgtactcg gcccggatgt cgtcgaagtc gtccccctgc ggcgcgatct caaagctctt
    29461 ggcgtactgc accgggtcaa ctttccgatt gaggaaagaa aactggatat gaatggaggc
    29521 gtccacatag ttatacaggt cgcacagttt ttcaaagata gccgtctgcg tgtccggctg
    29581 ggcaagctga tagctgatgt ccgcaaactc aatgcacttg gaaaagacgc cgggcgatac
    29641 ccggcacacg ccgtccgggt acatgacctc gtaggggatg ctctgctgcg ccgtgtgggg
    29701 cctgccgtcg cccttatatt tacggattac cgcgtcaatt tcttttcgct gggcgcgggt
    29761 cagctttgtt ttttcggttt ctcttgggat ttcctttttg gacaatggcc tttacctcct
    29821 gttccatctg gtattgccgc acaagggcgg tgtaggtgtt gtcggtctga tagggacgct
    29881 cctttggctg gatgaagcag cactggatga tctgcataat caccacttca aggggctggc
    29941 cgtgacgctc gtacatggca agcaaaaagc ccggcagcat ggcaaatacc atgagcaggg
    30001 ccgccgcgct gttggtaaga tagtcacggg tgagaaaata taaggggacg ccgatcacca
    30061 gcgccccgga aaaacagatc agttgccgtt tggtaagccc aaacacaacc ttgcttttca
    30121 catgggttaa gtccttgggg acggttacat aagccatttg gcacctcctt tcacgtcagg
    30181 ataaggtcac attccacctc gttcccccac acgtcccagc cgggggtttt ctgtctggca
    30241 aacagctcca cgcggggcaa atcgcccgcc agctctacga ttttttcacg ggtcacatcc
    30301 ggctttttgc tgtgttgttc aatcgggctg ataataaact gatggacaga ggcagaacga
    30361 cgcttcggct tgccgtgtgt tgccagcagg cagatttccg cgtttcccct tgtccagcgg
    30421 cccagcccgt aaaaccatgt aaggctttta cggttctgtt tcagccatac aaaagccacg
    30481 gtcttgaatg aaaagcccca cgctttgatt aaccgcagag cttccggcag catggggaat
    30541 gtggcccaca aaaacagcag gcagtctttg gacgccagcc gttccaccgg cagggcgcac
    30601 agctcgtcaa tgctcatggt ggaatagtga ttttccgctg cgccctgtac gtttttgcag
    30661 tcataccgcc acggcgggtc agcgtaaatg atgttgtacg ttgaaataaa caagttcctc
    30721 ctgtgtatgc ttggattttg ggatcaatgc gccccgaata cggcctttga tatactgccg
    30781 gtcttgaaca ggcagaagca cagcagcacc gtatagccca tacagcccca gattgcgccg
    30841 gatatgtcgg aagcggaacc gatattctgt atcagcaccg cgtagatacc caccacaacc
    30901 atgattaaaa aggcttggaa gcccagcgcc agcagggatt tcagataccc ttggcccatg
    30961 ccgcgccatt ccgcgttgcc cattgtggca agggggatag gccccagcga cgtgacaaca
    31021 tagatttcca ccatgcggcc ataggtcaca aggaaaatgc agatggaaag gatattcatg
    31081 gtgaggccca caacaagggt ttggaaccac agccccaaaa gagcgcccac gctcatagcc
    31141 tccaattggc tttcaaggtc tggtatcagg gtgttaaaat caatggatgt ctgcccgata
    31201 atcacaccgg cgctttgatt gacgacgctt tgggtggcgt cgaatacgcc catgacgatg
    31261 ttccatgtgt tcgttacgat caggattgca aacgcggatt tgaatatcca gcggaagaac
    31321 atggagctgt caaagtcgtg catattgttc ttttccgtta tcatctggat aagctcatag
    31381 cacatgacaa gggcgagtat ggccccggct atggggacga tcacggtttc cgacaggctg
    31441 tgcagcatat tgaaaatacc gctgttccat gcctgcgggg ttcgtcccac gtcggcggcg
    31501 atttctccga ctttggcatt taccgtatcg aacagcccgg acaggttgcc cgtgataccg
    31561 tccatgagga tgccttttat ccattcttca atgatttcac taatcacggg tagcacctcc
    31621 tttcccgcgc cctcccggtc tggccgggag gacgcggagg cgattagtta agggtttgga
    31681 cagggacaaa ggccaggatc agcccagcag cccggacagc aggggaacca gagtgacacc
    31741 gatcagggcc acgccgccgc cagcgacaag ctgcttgaca ccctgcgatt ttgcccccgg
    31801 attgtcctgc ccgtatccct ccaacagatt gatgccgccc catacgcaca gagcgccgcc
    31861 cagtccgatc acgatagtct gcaaagtagt aactgcggaa ttaaaaaatg ccataaattt
    31921 acctctttct gccggtttcc ggctcataat gattttttga ttttgttgtt ggaatgaaaa
    31981 aagccgctgt ttattcagcg gcggcgtcct cgtccgacag gtcgatttca tacaggtcga
    32041 atacttcatc ctgcgttacc acttgctgcc gtttccggcg cgtgatagtc aaaaatctgt
    32101 ccacgtcaaa ggtattcttc ttgtcggcgt cggcaaggta tttgtaccgg ggatgcttgg
    32161 taatatcgaa tttctccgat aaaaacgggc gcacaccccg tagctgcaag atacacttgc
    32221 cgccgtccat cacggcaatt tcgtcttggg acatcaactc ctttcctaat ttttgatagt
    32281 tcaatccgtg ggacgtttgg cttccccggt tctcgctggt gttgaaactg tcaatcgttt
    32341 ctttccccag cgcatcggaa atctccttgg catttttgcc gcgcccacta aggaaaagca
    32401 cactatcgca gttatccgaa atgatctcgg cggcgtcctt gtaaatcgct tttagctgcg
    32461 actggctctg caaaataata gaggccgata tttcacggct tcgaatggtg gcaatgagtt
    32521 tatcgaaatt gggtatttgg ccaatgttgg caaattcatc caaaatcagg cgtacatgga
    32581 caggcagccg cccgccgtac acatcatcag cccggtcaca aagcaggttg attagctggc
    32641 tttggagcat agccagaatg aaattaaagg tcgtgtccgt atcgctcata atgagataca
    32701 gcaccgtctt ttcgtccccg atggtgtcca gctccatttc gtcgtcctcc atcagctcgc
    32761 gtacctcccg gatgtcaaag ggcgcaagtc tggcaccgca tgaaatcagg atggaagaac
    32821 gggtcttgcc tgcggacagc aggaatttct tgtactggcg caccgcaaaa tggtcggggt
    32881 ctttctgctc caaacgctca aacatgagat cgacggggct ttgaaactcc gggtcgtcct
    32941 cgcgggcttc actggcgttt atcatttcca gcagcgttga gaagttcatt tcctccgctg
    33001 gggcctcgta ccagatatag ccgatcaggg ccgaataaaa caaccgttcc gacttcaccc
    33061 aaaaatcctc cgctgacttt tccccctcgc ccttggtgtt ggaaatcagg gtatttacca
    33121 gctttagcac gtccttttcc gaatggatgt agacaaaggg gttgtacttc atggacttgg
    33181 aaaagttaat ggtattcagg attttaatgc ggtagcccgc ctgctggaac atcctgccgg
    33241 tttccagaac caaagtgcct ttcggatctg tcaccacaaa cgaagtcgga tagtccgtgg
    33301 tcggaaagca ttgcatcagg ttgggtttca cgaaaaatct cgttttccct gaaccgctgc
    33361 caccgatcac cagcacgttt ttattccgcg ctgtcttggg gtctttgggc ctgccggtca
    33421 tggtaagccg ttccgtttgg gtgagcagga tattattttc aaacttgggg tcgatgtaag
    33481 gcgctatgtc tttggccgtc ccccagcggg cgctgccata ctccatgcct ttgcggtatt
    33541 tcttcacgtt cttgctcttg cagtacacca ccagccgcac gatcacggca ccggcgatac
    33601 cgatacacag gtcaaacagg tgcaggcttg gcagcgggtt ggcaaaagcc gccgcaaaac
    33661 catccgcaaa atgcaggagc ttccccgaca ggtcagcacc agcggccagc cggtacgcct
    33721 ccccgcattt cgcaaacagg tacacaaaaa tcagatacgg gaggttcagg atcagcaatt
    33781 tctttacgtc aggcttcaca gttccagccc cctttccttg attttctcct tggcccgctg
    33841 cttggtctgg gccgccgcct tttgcttcac agcggacaaa accttgcgga tagagggccg
    33901 ctgctccttg gaaagcgtct tggcggtaaa ctcccgaaac gccagatcaa ggttatctgc
    33961 atccttggcc ttgaaaatca caacgtagtg gggcggctgg gtggtcttat cttttcgtaa
    34021 ggtaaaatcc acgccgtatt tcttggcgca tggcttgaac gctccaatgt tggcctcggt
    34081 gatctcaatg ttggagagat cagccccatg ctttttaagc tgctttaggc tctgctggcc
    34141 gtggtgcagc gtgtttttgc ccttggcccg gtgttgcagg tactttttca gcgccgcttg
    34201 cagcacttga gccgtgagct tgcccgtttt catggacagg gcaatggtct tttgagtaac
    34261 ttcttcctgc aatcgcatcc ctccttgtca gaaagtcaaa agcggcgtct aaggcggaac
    34321 caccagacgc cgcaggtaga tataggtcaa atccgcaccc gcaggccgga aaggtcgtcg
    34381 gcccggatgc cgacaagata ccagttttcg gccatttcaa tgcgggtggg cctccatttg
    34441 ccgccgatca tcacgtcgaa acactccccg caatgcaggc cgccgtaata gtcggccaga
    34501 tcaaagcgga tgtcgtaacg gtctgttcgc tcgtcaaaaa tcaatgcgcc ctgcttcata
    34561 gggtcgtcct ccttttctcg ttacaggctt tcgccctcgc cgcaggaata gtccggcagg
    34621 ccggatttag gctttcccca atcgggctgt gccatgtcgt gcgcgacaag ggccgtgtag
    34681 tagttggaaa tggtgctggg ggcgttgaac agcatagccc gcaggtattg tttgatattg
    34741 cggactttcg ttgtgttctc ccgcagacag tcaaggacaa agcggatatg ttcgccggtc
    34801 agcttcatgt acttggattt caccagctcg gcagggtagt cgtccccggc gacacggatt
    34861 gtctttcggg cggtgcagac ggtttccagc atcaggtcta cgatttcgtc aagctgttca
    34921 cggtcaatgc tcctgtcctg aatgagatag gcatactcga tgttctccaa aatgatttcc
    34981 cgataaatcc gtacagcctc atcctttcct gtccttttcc gttccggcgg cgtagccgct
    35041 gcctcgccct ccataggggc aggggtcggg gatgggatag gaatggaatg ggtactttgt
    35101 ccatctgtaa ttattttttc tttttttgat aagtcagttc tttgtacttc tttatttaat
    35161 tgcgttggat tttccgacgc cggattatcc gatgtcggat tttccaacac cgggttatcc
    35221 gatgttggat tttccaatac cgggttttcc aataccggct gttcgtagat ggtatagacc
    35281 atatcggcca ttttgccttt tgcgtcacgg ccctgctcac gccggatata tccagctttt
    35341 tcaagctccc ataccgctgt cctgatcgcg tcgatgcttt cccggttgat ataggacagt
    35401 ccttttaggg tatagtccca atcctccggc aaggacaaca tctgcgacag caggcccttg
    35461 gcctttaagg tcaggttctt attccgcagg tggtgatttg acatcaccgt atagcctttg
    35521 ttcttttcca cacggaaaac tgccatagcg ttgttcgctc ctttcactgt gaatttgacc
    35581 gctgcatagt cggtcagctt gcccggctga tagggacact ctgcaaactg gcagaattga
    35641 tatttccaat ccgggcggta aaatcggcag gtgccgcaat cctccggcgc gtccgccccg
    35701 ccgccgtcgt agtgatcgaa accgggcttt tcctgcatca gccgttcaaa cgcccggtca
    35761 gggccggaag taaatagcat ggcgtcgcct cctttctcta tttgggcata aaaaagcggc
    35821 gtcctgttct cccatgacgg gaaagaggaa cgccgcgttt acggtgtcgt attcaattta
    35881 tgatgtgccg tatttggggt ggcgggtatt agcagtatgg ttcagcatat atgcggctca
    35941 atccgaaagt agtaaattgg tagtaaattg agttctcata tcctgcgaaa tgcccgtatt
    36001 tccggggctt tcagcgataa cctaaaattt tcatataaaa acatgaatag gaaaaatagt
    36061 tgacaagaaa atgacaacgc catatgatgt tccatagtat ttacaacctg tcatagatga
    36121 ctgtaaaaaa taaaagtaaa aagaaaggac gacaacaggt ggatttttat cagtctctac
    36181 aactagatcc gtttatttta aaacaaaaaa tacgagaagc agcttctaaa aaagaaaagt
    36241 gcatgtatat ttgttcattg tttttacgat cattattcat cgtattgttt gccatttgtt
    36301 ttatcattat aattacaaca ttatttgaat cgaagcataa accatacgct gtggttttat
    36361 tctgtatgtt gatgagtatt cgttttgttg actttggtta taagatttcc cattcgatca
    36421 taggtttagc aatcgtcatg ttctcactat tagtagcacc gtatgtccag ctgatcaaat
    36481 ggtcagttat gggcatgttg attcatttta ttttactaag cagcatatta atggcaaccg
    36541 ctagtgatcc caaaatgggg aatgcttctt tgtacggctt ttcatatctc tttattgttt
    36601 attcgttacc taaagattcg ttgaacaaga atttctttac acaaacaagt agcttattat
    36661 ttttgttttt ttgttggttt tcagttctcc tttacagaaa acatcgagaa aagaacaaag
    36721 acaaaagctt gtttaaagag atttttttga aaggcatgta ttcgcaggaa aaaatttgga
    36781 tgctcatcta tgcatttggc attagtcttt tgatcgttgc aggagagtat gtcccttttc
    36841 agcgattgat gtgggcaggg tttgcatttt cttctattgt atctagctat ggattgatgt
    36901 cgattggttt caaagaacga gcagtagacc gaatcattgg ctcattgatt ggatgtgttt
    36961 tatttattgg tatcagccaa tttattcctt ttaactgggt cggtatatta ggtggtctag
    37021 ctttaggtgt ttgttctacc tatagatata aaacagtttt taattctttt ggtgctttgg
    37081 caatcactgc cagtttattc ggggttccag gagcagtaac gatccgcatt tttgagaata
    37141 tccttggggt atgtttagga atcatgtata ttggggtgac tgaaatactt atccgaaaaa
    37201 taagagaaaa acacggtttg aatcattaga tgagatcgaa taaaaaataa ggaaaaagtg
    37261 aagatacaca tggatgataa attgctatca ttccatgtgt atctttttac ggtaaatcag
    37321 tgaaacgttc gactttcatt tttaatttat actttttacg gagttcgttt atttgagcca
    37381 tcatttcgga tttgtgatgc ttttcgattg cttcttcatc tttccaacgg tcgatcagta
    37441 aaagagattc tggatcttct agtggaaagt aatagtcata tttctcgttt ccttcttctg
    37501 cacgaatcgc tttgacaatg tttctttcga ccatttcttc tgcaaatttt ctggcttgtc
    37561 catttgtact tgtatacaaa atattcactg tgatactcac taaatcccct cctgaaaaaa
    37621 ttctatcact agtatatcac ggtgccagaa attattattc aagatatgat acacattttt
    37681 tccgcaatgc tacggaaata tatattttga atagaaaacg cttacaagat atgcttggtt
    37741 tataaaaata aaaggagtga ttgatttatg aaaaaagcat tgattatttg tgcggcagga
    37801 atgtcatcct cgatgatggc ctcaaagaca acggatttct ttaagcaaaa aagagaagag
    37861 gtactcgtag atgcagtttc agctacagaa ggcgataata tgattaagaa tagtgatttt
    37921 gatttatttc tgatcagccc gcaaacgacg atgtttttgg ataaattcaa aaaattagga
    37981 gaatctgttg gaaagcctgt tgttagtatc cctttccaag cgtatgtccc aatccctaca
    38041 ggcatccaga agttagcaga ggtgatcgaa gagaacatat gatggaatag gagtggatga
    38101 taatgaaaca gaaggaaaaa ttgctgttga gatatttgat tgatcacaag aaggagtatg
    38161 tcaccagtca acgtttagct tctgaattgt tattatctga tcggacaatc cgtaattact
    38221 tgcagcggat caaagagtta gtagaaaaaa acggaggtaa gatcattgct aaaccaggtt
    38281 atggctacca actgcatatt ttacaaagac tgacatttga cttgtttttg agtcggcaag
    38341 aaatagtacc tggatatgct agagaaccgc aaggatttta tgagtccgaa gatcgacaaa
    38401 agtatatatt gaaccaattg cttttggaag atgccattct ttttatggat gatttggcag
    38461 aagagcttta tatcagtcgt tccagtttga caaaggacat gcaagaaatc aaggagcgat
    38521 tgattccata ttcactaaaa atcgtttcta agcatggtca agggacatgg atcgaagggg
    38581 aagaaagaaa tcgacggcat tttatcatgg atactttttt tggaaaccat tatggaaatt
    38641 cattgaaaga atatttaggg caaagccgtt ttttcccaga catcagtttt gaagagctag
    38701 tcattatcat tctcgatgaa acaagagaag ctaagttaaa agtttcggat tttatcatac
    38761 agaatctgac tttacacttg gctttgggga tcaaacggct ccgagaaggg tttgaaatca
    38821 aggaattagg catcgaacaa gaagtttcta agcgagtaga gtatcaagtg gcgcagcgga
    38881 tcgttcaacg tattgaggta atcgcaggtg tatcgtttcc cgaagaagaa attgcttatt
    38941 tgacattaca tctcatggct aagtcgaatc acaaagaaaa tcaaaatgat caggaactat
    39001 caaaggaatt agcgaaagtt ctgaaaagat tgtctcagga atttcatttt tcattagtag
    39061 aggatctcca gcttttcaat ggactactcg atcatttgtc accgatgatg attcgattga
    39121 gccgaggtat tcgcattgag aacccattga ctgaggagat aaagaaagaa aatcccaaag
    39181 tctttgacgc agtaaaaagg catttcagca acatgccggc tttgaaaaac tataccatca
    39241 atgaagatga atgggcctat ttggctttgc atctcatggc ggcattggaa aaagaaagag
    39301 ctgcgcataa actacatgca ttgattatct gtgcaacggg ttacggcagt gcccaactat
    39361 taaagaatcg cgtagtcagt gaattcggta aaaatattac agtggtaagt gtaaaaggct
    39421 attatgagat caatgaaaca actttaaaag gaatcgacct gattatttct tctattgatc
    39481 tttcgacaat gtttttcaaa attccagttt tacatgtcag tatttttttg aatgaagatg
    39541 atgttcgtaa aatacgaaag gtagttggag aatctatacc ctatcattcc tcagatagac
    39601 cgataccagc attttctcta cagcaaaaaa agcagattta caccgaacag ttgtctgaaa
    39661 aatttttcaa gacttatacg tacgaaccga aaaaggaaga aattcttcat gatttgctgc
    39721 aaaagctgag cataaatgaa gaagaaggat atataagaga aatgcgaaag cagatccagc
    39781 aacgtgaaaa tatgggacaa atcatattca gtgatacagt ggcagtccca catcccgctg
    39841 ttccagttgg aatcgaaaca aaggtagccg ttgcattgat tccttctggg atgcagtggg
    39901 atcaatatca agaaattcat tttgtttttt tagtttcgcc ttcttatgtc gaaaatgaag
    39961 gaattacggt cgtgactaaa gcaatcgtta ggttagttga ccagctaaaa gtacagcagg
    40021 aaatcttagc tgaacctatt tttgagaatt tcagcagtaa atttatcaaa ctgatcgaat
    40081 aaggaggaaa acagatggct gaaaaaataa acagtgaaac actacaagtg acttcttttg
    40141 atatcatttt ccatagcgga aatgcacgga cactgataca tgaagcattt cgctcgatgc
    40201 gtaaagagat gtttgaagaa gtagaagaga agttgcgaca agcaaatgaa gagattcttc
    40261 atgcgcatca gtcacaaact caacttctga aagaatatgc cagcgggaca aaaatagaaa
    40321 tggaaattat catggtccat gcacaagatc atctgatgac gacgatgaca ttattggaag
    40381 ttgcacagga aatgaattat ttgtatcaaa aaaataaata gaaaggaagg atcattgtgc
    40441 aggatacagt aaaaaggaaa atcgaattag aaaaaaagca acttagtttg aaaaacatgt
    40501 atttcaatcg ttatctaatc gtacgttacc tgactgcctt tttctttttc atgaacatgc
    40561 aatggatgat tctgcttcta tcagctaaaa gtttaggaag tagtttaccg atgattctga
    40621 ttttagctat tttgcctgca gtaggagaac aagtaaagct ttatcgaaaa caccaaacga
    40681 acgttccatg gacaaagtgg tattttttgt tccagagtgt ctttaattta ttagtgattc
    40741 cagtgttgtt tacctcagga tttacattct tgtatccttt tatggcaaat aacaatggag
    40801 gacagttatt tgttttcatt cttgttgtaa gcggtatttt tgccagtgca tttatacagt
    40861 atcgtttaaa gaaaatttca ctcaaccaag accaacagta tatccgtatt aaacaatatg
    40921 aaaaagcgtt atatttagga aaggagaata actaatgttt gattttttgc agaaatacct
    40981 gatgggacct atggggaaaa ttgcgcaact taaaatcgtt cgggctgtca tggcagcggg
    41041 gatggcttct attcctttca cgattgtagg ctcgatgttt ttggttttaa atattttgcc
    41101 attaccgttc ccatttttag aaggattttt taatgcaact tttttcaaag ttagtgactt
    41161 gtacatgatc gtcaatacaa tgacgatggg gattttatct atttactttg ccattgtgtt
    41221 tgcttatgaa ttaacgtcaa ttgaacgaga tgaacaaaaa ttaaatgtta atccgctcac
    41281 gggggcatta ctatctgttt ttgcattctt tatgtgtatc cctgaattga tcatgactga
    41341 tggtaaaatc agcttgattg cttctatcaa tgagacagaa actgtagtaa atggtattag
    41401 gatgggctct tttgtcgaac gtttaggaac ttcaggaatc tttacagcta ttattatgtc
    41461 gattattgcg gtcgaattgt attgtatgtg tgtcaagaga aattgggtca taaaaatgcc
    41521 agatgttgtt ccgccaggag tttctcgttc tttcactgct ctgatcccaa catttgtcat
    41581 tgcgtttgtg gtgatgatca tcaatggatt attagttgca ctaggaacgg atatcttcaa
    41641 aatgattgct atcccatttg ggtttgttac gcagttgacg aatacatggc ttggtgtcat
    41701 ggtgatttat ttcttgatcc atgcattgtg gatcgtaggg atccatggag cgaatatcat
    41761 cacttctttc ttgaccccga tcgtattagc taatatggca gctaacgcac aaggcgcaaa
    41821 ttatccgttg gcaggagaat tcaacaattc ttatgtaacc gttggtggat caggtgcgac
    41881 tttaggattg atcatcttta ttgcattcct cgcgaaatct gatcagctga aagtgttagg
    41941 aaaagcctca cttgtaccag gattattcaa tatcaatgaa ccaatcattt tcgggatgcc
    42001 aatcgtctac aatccctatt tagcggttcc gtttttctta gctccgatgg cttctgctac
    42061 actcgcttat tttgccattc ggctggaaat cgtcaaaccg atgcttgcac agatgccgtg
    42121 gccttcacca gtcgggatcg gagcatttgt tggtagtggt ggtgattgga aagctgctgt
    42181 gttagcagtc atttgtgcaa tcatcgcatt cattatctgg ttcccattta tcaaattcta
    42241 tgataataaa ttattaaatg aagaaagaga aaaggcagca gaattagcta gtgaaaagtt
    42301 aaaatgattt ctaagaaaaa aggaactgtg acaaaaaatc acagtccctt tttttcttag
    42361 aaatgggaaa ccactagtat ataaatacca ttattcttca atcaaccgct tcacgattgc
    42421 ataatcccct tcataaggtt catctacacc attgattttt tgatagggat ccacgccttt
    42481 tcccgcaata acgactgaat cttgcggagc gctttctgct aaagcgcgtc gaatcgcttc
    42541 aggacgatcc atttcataat gaacgatcaa atctggatta gtaattgctt cattgatttc
    42601 ttccgcgatt tttttcggat cttcaaatgc tgggtcatct gccgtcaaaa aggcgatgtc
    42661 agctgtttct gatagcactt ttccaaaatc atgtcgacgg gagattgctt tgtttcctgg
    42721 gctgcctaat acaacaatta cacggccatc tggatgttca tttttggcaa atttcagcaa
    42781 tgttttcaag cttagataat tatgtgcata gtccacatag acatgcgctc cattggattg
    42841 gatcaattga tccattcggc ctggtacacg agcttctttt aatccgtctt gtgcatcttg
    42901 actggaggca cctgttagtg ctgcggcaat gatcgcactg gctgcatttt cttggttgaa
    42961 tcctccagcc agcaaaatat cataagcagt atcgcagata gctaagcgat cttcatgaga
    43021 agataaagta aaaccgtgag tacctttatc tgaacggatc acttgataat tgctttcttc
    43081 cgaacgacca taagtgatcg ttggaatctg gaatagatcg gctgtttccc taagtaaatc
    43141 aaaatagtca ctttcatgat ttaaaacaac tgtttttgaa ttcaagatca attgtcgctt
    43201 gcaatagaaa tagtcatcaa atgtcggatg ttcgatcgga ctgatatggt caggcgagat
    43261 attcaagaag attccgacat ctaaagtcaa gccgtatacg cgttgtgttt tgtaagcttg
    43321 tgaagaaact tccatcacta gatgagtcat accattttca actgcttctg acatcatacg
    43381 atataaatct aatgactcag gcgttgtcaa atgggatttg aaatatgttt tgccatctaa
    43441 cgttgtattc atagtagaaa gcaatgctgt ttttttattt gttgtgtgat ccaagattgc
    43501 ttttgtaaag tacgcagcag ttgttttacc ttttgttcca gtgaatccga tcactttcaa
    43561 tttattttgc ggatagtcgt aaaaagccat agcaagaagc gccattgctt tacggatatc
    43621 cgttacgatg attcctactg ctgaattttc gtatggttgt tctgatacat aatattgaat
    43681 accctgttcg attgctgaat ccaaatattc ttctttgaaa ttcattcctt tacagaaaaa
    43741 caaagtagaa gcatcagctt ttcttgaatc ataacttaac tgaagaaatt cagctttttc
    43801 tggtacatca agatgccagc ctcttgttga aacaaattct tttaatagat tttcttttaa
    43861 tagtaaatga cggatgtctt ctaaagagaa gctcattgat tgatccctcg ctttattcaa
    43921 tttcttggtt tattatagca cactcttttt aatatatcgt ttgtttccgc tatgacaaaa
    43981 tccagcttta ttcaaatttt cttgacacag gggacaagcg gatgagtatt ttcataaaca
    44041 atgaactgac tgtaatagat aggagaacga atagtgtctc caacaatcca aaaacataat
    44101 aattcaaaaa gacgataaga ctattggtaa gtaaaagtcc ttgcggcatt ggaagccgaa
    44161 aaaatttgtg gaaaataatc ggcggtatcg ttataccgcc atttgaggcc ccgactgtgt
    44221 acaatgtgta aaccccaaga ctgaacaaaa gacttcctga aatcagtgaa aagaaatgtg
    44281 aggagatcat ttccactttt ggaacaatct ctaaaacaac aggaaaaagc agacttcccg
    44341 ctacagtctt aaaaaatact gattttccta ataggacagc ggctagaagc agcatcataa
    44401 gattgatgct ccaaacagta actgagcgtt ggatagaaaa ggccgtttca actaaatgtc
    44461 cgatggcgcc cactccggcg gaagctatat cgtgagggag caagaaccaa ttcaatccaa
    44521 ctgtgataaa gaaaagacct aaaaatataa cgggtagttt ttttatttct tgtgtcattg
    44581 atatctccta taaatgttag ccataaaagg ctgtatattt ctttacttta ccaaaaaaac
    44641 gaaaccgaat tcaaaaaaac aattgttctt ataaaagtta tataaaatcg attgccagtt
    44701 tctgttttac caattaaaat gatagtaatg ttttgtagaa agttggaatc tgaccgatgg
    44761 ataatcaaag aaaaccagca ttagatcaat taacaccgca agaaaaaatg gcacgaggct
    44821 ctgcttggat gaccgccagt aatatcattt caagattgct aggcgctgtc tatatcattc
    44881 cctggtatgc ttggatgggc gaaaatgcta aggctgccaa tggattattc aatatgggat
    44941 acaatatata tgcattgttt ctactgatct ctacagcagg aatacctgct gcgattgcaa
    45001 aacaaactgc acgctataat tcattgaatg aatatggcac ttcacgacga ttatttatcc
    45061 gtgcattaca gatgatgggc gggttaggcc tgttgtttgc tttgttcatg tacatcgcct
    45121 ctccttggct agctcatgct tctggtggcg gagaagaact gattccaacg atgcgttctt
    45181 tgagtatcgc tgtattggtt tttccttgta tgagtgtgat acgagggtat ttgcaaggca
    45241 atcaagaaat gatgccttat gcgctgtcgc agattgtaga acaggtggca cgtatttttt
    45301 atatgctatt ggctacattc atcatcatga aagtcatgga tggtgactat gtgacggcgg
    45361 tcacgcaatc aactttcgcg gcatttatcg gtatgctagc aagtattctt gtccttttgt
    45421 attttttgaa gaaacatcaa gcgtatacat cggcgtttat tcactacagc gaaaacaaaa
    45481 taaatattgc aacgaaggaa ttgttgttag ataccgttaa agaagcgatt ccgtttatta
    45541 ttgtgggttc aggggtaacg atcttcaaat tagtcgatca gttcactttt atgaagatca
    45601 tgtctgcatc caccgagtat tccaatgcac agttactgga tcttttttcg atttttagtg
    45661 ccaatccaga taagttgacg atggtagtta ttgcattggc aacatcgatc gcgtctactg
    45721 ggttaccgtt gattacagaa gctgttacgt tgaaagatcg agtaggtctg gcaaaattga
    45781 caagcggaaa tttacagttg ttttcatttt ttatgtttcc ggcgacattt ggtgttatgt
    45841 tacttgccta tccgctaaat acattatttt atacaccgga ttctctagga agtaatgtac
    45901 tgatccaagc aagttttgta ggactcttcc taggactata tatgctagtt tccaacatgc
    45961 tgcaaggaat gtttgaaaac aaggcggcaa ttaattattt ggttattggg tttctagtga
    46021 aactccttct tcaataccca gccattcgtt tatttgaagt atacggccct ttattggcga
    46081 caatgatcgg atttgctgtg tcttgtacct tgatcttaaa gagaatccat gaagtagccc
    46141 gctttaaacc aagttttgtc tggcgcagga cattgctgat ctttattctt accttgatta
    46201 tgcttttggc ttcgtggatc acaaagatga tttttggtgc ttttttaaac gaagatagca
    46261 aattccagtc tctattgttg attcttgtag tagcaggtat aggggcttta gtgtatggtt
    46321 atttagcttt gaaaatccgc ttggcagaaa gactactagg accaggaata gcgcgtttac
    46381 gacataaatt aaaaataaaa taagtgaatc agaagagcta gattgatgct cttttgaaat
    46441 gggggaaacg catgcgttta gataagtttt tagcagatat gggacttggc agccgcaaag
    46501 aagtcaaagg attaatcaaa aaaggaacga tcgaagtcaa tggacaagtg gtgaaaagcg
    46561 ataaagcgca agtaaatgaa ttcgaagatg cggttactta tttaggagaa atcattacgt
    46621 atcaaaaaga tttttactac attttgcata aacctgcagg agtcatttct gctactcagg
    46681 ataatcatga tcaaaccgta atcgatttat tggaagacca ggattatcgg gaagatcttt
    46741 ttccagtcgg ccgattagat aaagacacag agggcctctt gattttaacg aatgacgggg
    46801 tgtttgccca tcagttacta tcacctaaaa aacatgtgga gaaagaatat ctagctgagg
    46861 ttagcggtat aatgacagaa gaagatatcc gtttatttgc agaaggactg attattgatg
    46921 gcgatgagca gacattgccg gcaaaactcc aaattgatca agtagatcaa gagacagaaa
    46981 cttcaattat ccgtctgatt ttgcatgaag gaaagtttca ccaagttaag cgaatggtca
    47041 aagcagtagg aaaagaagtc ttatatttaa aaagaatccg tatgggaaaa ttttggctgc
    47101 ctaaagattt gaataaaggg caatatcgac ccatgaccaa agaagagcta gaacaagtga
    47161 gagaaaaata attgaatgga tagctaaaag aataagcaga gtagttgtag cgccatctga
    47221 aaagcagact tgtccgtctg actttcggat ggcttttttg tgaaatttcg gcttatttcc
    47281 gtagaagcta cttctggaac accgtttgtt cggaataagg gactgtgaca agacttttgt
    47341 tacagtcccc attgttcctt gtgactcaga gttagatagt aaggacacga tctccctaat
    47401 actcaggcca aaacgctgaa ttcacaacct ctgataaacg gtgttcaatc agctgaccct
    47461 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttcccgtttt tttctctaat
    47521 actcaggcca aaacgctgat ttcacaacct ctcctatact ttgtttcatt ttccctatac
    47581 tttgtttatt tattctcatt attgaaagcg ctataattac gttgcgaatg aatcgaaagg
    47641 ggtttttaga tgaaaaaacg aaaggggttc aatcattttt ttatgaatct atggcgttat
    47701 aaagcacttg tcctgatggc tgtaccgggt atgctttgga tgattttttt cttttacata
    47761 cctgtaatgg cgaatgtcgt agcattcaaa aattttcata tttcagcaga tgggttcttg
    47821 gctagcttga gggaaagtcc ttgggtgggt tgggaaaatt tcaaattttt attttcttcg
    47881 gatcaagcat ttctcatcac taaaaataca attttatata atgtagcatt tatccttttg
    47941 aatctgctga tctcagtcgt atttgcaatc attatgagcg agttgagaaa taaacgactc
    48001 gttaaagttt atcaaacgat gtccttacta ccttattttc tatcttgggt cattatcagt
    48061 tactttgttt acgctttttt aagtcctgac aaaggaatat tcaatcagtg gatcactgga
    48121 catggaggag aagctgtcaa ttggtataat gaaccgaaat attggccgtt tatcttggtg
    48181 ttcattggta catggaaagg gattggttat aacagcatta tttactttgc atctgttatg
    48241 ggaatcgatc cgacttatta tgaagctgct atggttgatg gagctagcaa atggcagcag
    48301 atcaaacatg tgacgatacc acaacttatt ccactaatga cgattttaac gattttagcc
    48361 gtaggaaaca ttttccgagc tgattttggc ctgttttata acattcctag gaattccggt
    48421 gcattatatg atgtgactca agttttggat acgtatatct acaatggcct gacatccaca
    48481 ggagattttg gtatgacggc tgctgcagga ctttaccaat cagttgtcgg ctttgtcttg
    48541 ttgatgatca caaatacgat cgcccgacgt tttgatagcg attctgcatt attctaagga
    48601 ggaaaaaaat gaaaaaaaag aaagtggaga gagtccaagt ccgctctttc aataagacgg
    48661 ctaatttttt ctctaatata atgattgcac tctttgctat ttcctgtatt ttgccattta
    48721 tatttgttat cgccatctct tttaccgatg agtccatatt gtcgactcac gggtacagtt
    48781 tttggccaga aaagttcagt atatttggtt atacgtactt gttcgaccag atgcaggata
    48841 aaattttcca agcgcttttc gtcactgtac ttgttactgt tttaggtaca ctgatcaata
    48901 gcacggctac ttctttatat gcttatgcga tttcacggtc aaactttcca ttccgtcgtt
    48961 tttttaccgt tttttgtttg attacgatgt tgttctcacc agggatggtg gccaattatc
    49021 ttgttatgac aaatcttctt cagttaaaag atacgatttg ggcattgatc ttgccaatgg
    49081 ctgtcagtcc cttcaatatt attgtgatgc gcacattctt taaacgttcg gtctctgatt
    49141 cgattattga atcggcacgg atcgatggag ctagcgaact gcgaatattt gttcagatcg
    49201 ttttaccgtt agctgttcca ggtattgcga caatcagttt atttgccgca ttgggttact
    49261 ggaatgactg gttcaatgct ttactataca tccaagatga taagctagtt ccactacagt
    49321 atcttttgat gaaaatccaa agtaatattc aatatctgac acaaaattca ggagcaggaa
    49381 gccaattgac aggcggatta gcctcagttc caggagaatc tgccagaatg gcgatcgtag
    49441 tcatctcgac gttgcctatt gcgatcagct atccattttt ccagaagcat tttgtcaaag
    49501 gtctaacaat cggcggagta aaagaataaa gaaacaaaaa tataaatcat aaaattagga
    49561 ggaagaaaaa tgaagttatg gaagaaacta gcctttacag gtgtttcagc gttgttgttg
    49621 ggaacattag cagcatgcgg aggttctggt tctaaagacc aggcagaagc tagcaattcg
    49681 gacactttgc agatgtatca aatcggagat aaaccagata atttcgatca attgatggaa
    49741 gtagcaaaca aacgaatcga aaaagagatc ggtgtaaaag tcaatatcaa ttacattggc
    49801 tggggcgatt acgaaaagaa aatgaacgtc atcatttctt ccggggaaaa ttatgatatc
    49861 gcctttgcga acaactatgt ttccaatgcc caaaaaggag cctttgctga tttgactgaa
    49921 ttagcgccta agtacgctga aaaagcttat aatgatttag atgaagcata tattaaagga
    49981 aatctggtca atggaaaatt atatgctttc ccagtcaatg gaaatgtctt tgctcaacaa
    50041 gtacttactt ttaacaaagc tttactagat aaatacgatc tttcaattga tggaatccaa
    50101 tcttacgctg atgcagaaaa agtcttgaag gaattccatg aaaaagaacc aaacacggca
    50161 gcttttgcca ttggacaagg cttcaaagtc caagggaact ttgattatcc tttagggaat
    50221 acgttaccat ttgctattga tctggatgga gatgattcga aaatcatcaa ccaatacgat
    50281 aatgaaacgt atatcggact gttacgtaca atgcatagct ggtacaaaca aggactcatt
    50341 ccaagtgacg ctgcaacgag caataaggat tatccactag aaggaaatac ttggctaatg
    50401 cgtggagaaa cacaaggacc gtatgattat ggcgatacaa ttttgacaaa tgcagcaaaa
    50461 caagaacttg tctcaaaagc aattactgtt ccactaaaat ctacagctca agcacaaatg
    50521 gcaaattttg tcgtatcaaa tacttcaaaa aataaagaaa aatcagtaga acttctaggg
    50581 ctgcttaaca gtgatccaga attattgaat gggcttgttt gggggatcga gggagaagca
    50641 tgggaaaaag tcgatggttc agatgataaa atcaaattgc tagatggtta tcagccaaat
    50701 acacatatgt ctgcttggaa tacaggaaac aacaagattc tatatacaca agagtctatt
    50761 acggatgaca tgattgccaa acgtgatcaa tcgattgctg atgcagaaac ttcaccgatt
    50821 ttaggattca gcttcaacac tgacagtgtc aaaacagaat taagtaatat ttctaatgtc
    50881 atgaatcaat atttagatgg attgaataca gggactgttg atccagatga aacattaccg
    50941 aaattgaaag acgctttgga caaggctggc tatgataagg tattgaaaga aatgcaaaaa
    51001 caatatgatg agttccgaca agaataataa tgaataaaga caaaggggtt gtgacaaaag
    51061 acgtgtcaca acctcttgtt ccgaataaac ggtgttcaaa agccagtttc tcggtattaa
    51121 gctaaatata tgtaaaaatg tggaaagcca tattcacaaa atttttctta ctactcgaaa
    51181 ctaagcggct atttcacaaa ctcttggtaa acggtgttca atcagctaac cctcggtatt
    51241 cgctcacaaa acgacaaaat atgaggaact atttttccgt ttgtttctct aataatcagg
    51301 tcaaggtgct gattgcccaa cctttttttg tataatgaga aagtataaac ttaatgtttg
    51361 gaggagaaga tatgttaggg aaggttttgg aagagttatt tatccggatc tgggtcatta
    51421 taaaactaac cttgtatttc tggatatata catttgccgg cgggatcatc tttggattag
    51481 gtgcggcatg gaaaacagtc aatgaactat tttatttgta cggattcgag tataaagaaa
    51541 tcacaataaa aagaggctgg aatatatata aaagaaattt tttaagaggc aatctgctat
    51601 ttagtttatt tttatctggt actgctttat tgagttacaa tttatattta tctgttcaaa
    51661 tcaaagggct cattttttta gctattgatt ttattttact ttttgccttg ctctgcctat
    51721 ttgctactta tcaatacagt atgattttag atagtgaata tacgatctct gttcctaatc
    51781 tgctaaagct aagttttatt tcggtttttt ccagcttttc gtcttttcta aaaataatta
    51841 ttggtagcgg tataatttta ggcgtgactt ggcaattcaa aggattgatc ctgtttggtg
    51901 tcattggtct tttgacggtt tggaatggta cgatgacaac acattggcgt gaagaattag
    51961 ataaacagtt ggaaagttat gaataagttt cggattctat tttcaaaagg aaccttacta
    52021 aatcgtctgt tgcggaaata cgcggttatc atgatcggtg taacgatgac tgctaccgtt
    52081 atttttagta tccatacttg ggaacaaaat caaaaacagg cagaaaacat gacaagtgat
    52141 gccgttcaaa gcacaagcag aatgctgaat gataaaacaa ccttgagtag gatcatcaaa
    52201 aaccagcttg tagggaattc cgaaaagatc gaaaatgtga cgacctactt aacaaaaccc
    52261 atagatcagt atctgatgta tgtatacgaa cagcaaaaca gtaccgatga attagtttct
    52321 ttcccaaatc aaatcaaaga tctctacgca aattatgaag aactaagtgc gatatatatc
    52381 gtgttgaatc aactaccgga gtattatgaa tcttctagag aaaacaaagg tggggaaata
    52441 aaaaagggga caccaaaatt actagacgcc ttttatatca agttgcctat cacacaagga
    52501 ggagctgagt caggaagtat ttttattggt tttcaaaaga aagaactaga tatgatttta
    52561 gaaaatctaa cgtcattcaa tggactctct ttatacatga tttctggaac gacaaatcga
    52621 ctatacacat tccatgataa taaaatagct aaagaaacgt ttgaaagaca agaaatgatc
    52681 atcacagaga gtctgaagaa aaattcacaa ttgccgattg acgatctgga aaaaaacaat
    52741 tttgttcaac accaagaact gtctgatgat tatcgtatat tagctgtact agataaaaaa
    52801 agtgtaaaaa tcgaaacggg aaaaaatctt gccccgctga tcattggtat cattatttta
    52861 gacaatatac tgcttatctt tctttataaa acattcaaac gatattcaca gcaagtatct
    52921 atcgtcatgc aggccatgga tcaaacagca gcagggaatt tagagacgcg gatcgacata
    52981 acaaacacgg aatatgaact gaaggaactt tcaatcggta tcaacgaaat gttggatagc
    53041 atcaatcagt ttgtagaaga catctacaaa ttagaaatca aacagcaaga cgcacatatg
    53101 cgggcgcttc aagcacagat caatccacac tttttatata atacattaga atatatccga
    53161 atgtatgcaa tcagcgaagg aagtgaagaa ttagcagatg ttgtgtatgc gttttccgct
    53221 ttgcttcgaa acaatacgaa tcaagagaaa acgatcactt taaaagaaga actggacttt
    53281 tgcgaaaaat atgtctattt gtatcaaatg cgttatccta atcgagtagc ctatcatttt
    53341 atgatcgatc ctgatttgga aaagatcgaa gtacctaaat ttgtgatcca gccattagtt
    53401 gaaaattatt ttaaacacgg tattgatttt actcgattcg ataatgcgct aagtgtcaaa
    53461 gtcttgcagg aaggcaaacg ggtaaggatc atcatcaaag ataatggaaa agggatgact
    53521 gaaaaacggc taaaacagat tgaagaaaaa ctgtctcatc ctaaagtgga acttcacggt
    53581 tctatcggtc tgcaaaacgt gaatgaacgt ttacgtgcca gcttcggcag cagttattat
    53641 atgtcgcttg agaataatga gacaggtgga ctgaccgttt ctattacatt caaggaggga
    53701 tgagcgtgta tcaagtgtta ttggtagatg atgaatacat gattttgaat gggttaaaaa
    53761 agataattga ttggcaaagt ttaggctttc aaatagtagc aacagcagag aatgccaaag
    53821 aaggactagc tgtacttgaa cagcgacaga ttgatttagt agtaacggat gtgacaatgc
    53881 cagaaataaa tggactggaa tttattgaag cggctcaaaa agaacgtcat aattttgaat
    53941 ttatgatttt atcaggatac caagaattcg attatttgaa aggaggtatg caattaggcg
    54001 ccgtgaatta tttgatgaaa cccgtgaata agttcgaatt ggttgaaagc ctcaagaaaa
    54061 tcaaaacacg gttagatcag caaaatgaac aaaaaaatca acaagaaatc tatcaagaac
    54121 tgttattcag tcaatggttg aatgaagaat tggatgaaat aagtgaagaa gaaataatag
    54181 gacaaataag tgagaagcag cataggatag tattgattca actggcaaga actcatgata
    54241 ctcttatcaa tagatggttg caagaacggc aacaaatctt ttactatcaa cgaaattacg
    54301 gagaccgtgt attgatcacg ttgttgttgg aggcagatga agttgatggc ttctgttgtt
    54361 ttgttcaaga aaactttcct gatcaggaat ggctgatcag tatcggggaa gagacagatt
    54421 ctttggacaa aatccctgaa acattccaac aagcgaaaga caatctacag ttgcatcaat
    54481 tttatggaga taaagacgag catgtttttt acgcagtaca agcagaaaca aaagatcaaa
    54541 ccatcgattt ttcaagtttc agacgcgtgc tgcaaaataa acaattggaa gtcgctcaaa
    54601 aaatgatcac tgattttttt gaacagtttc agttagcagc catgatgccg gaagatatac
    54661 gatattccag ttttttactg tttatggaga ttcaacggga attgattgat ctggaagatg
    54721 aggaatattt acaaggaatc gaaaaaatcc atcaggcaaa aacggtgcaa gaacttcagc
    54781 agctacttct gtcattcatc aaaaagcatc agagacagaa aaagtacagt gacaatgtgg
    54841 aaaaagtaat cgtaatcctg caccagcatt atcaagaacc acttacactc aaagaagtgt
    54901 cagaaaacct gcatttaaat gtcatgtatc taggacagct gttcaaaaaa gaaacgaaaa
    54961 aaagtttttc agcttatttg aaccatttac gtatggaaaa agcgaaacaa ctgcttttgc
    55021 acagtaatca aaacatcaat gagatagcaa gtgagattgg ctacaacaac accacgtatt
    55081 tctctaaatt attcaagaaa atcgtcggca gatcccctaa agagtaccga gaaaacagtg
    55141 gagaatcata aaagaaaaag ccaatgtaat gaaataactt ataaaaaatt gtctagaaat
    55201 ctaattttgg gctgttctta ttacttgagg caaggcagct ttttcatgac ctctcgataa
    55261 acgg
//
LOCUS       DAYYAO010000007        54587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000007 DAYYAO010000000
VERSION     DAYYAO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.007"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..199)
                     /locus_tag="ACHARI_000355"
     CDS             complement(50..199)
                     /locus_tag="ACHARI_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441437.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1167..1493
                     /locus_tag="ACHARI_000356"
     CDS             1167..1493
                     /locus_tag="ACHARI_000356"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8441438.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1501..2409
                     /locus_tag="ACHARI_000357"
     CDS             1501..2409
                     /locus_tag="ACHARI_000357"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8441439.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3035..3289
                     /locus_tag="ACHARI_000358"
     CDS             3035..3289
                     /locus_tag="ACHARI_000358"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFM8441440.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3831..3989
                     /locus_tag="ACHARI_000359"
     CDS             3831..3989
                     /locus_tag="ACHARI_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441441.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4061..5286
                     /locus_tag="ACHARI_000360"
                     /pseudo
     CDS             4061..5286
                     /locus_tag="ACHARI_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5375..5767)
                     /gene="rpsI"
                     /locus_tag="ACHARI_000361"
     CDS             complement(5375..5767)
                     /gene="rpsI"
                     /locus_tag="ACHARI_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8441442.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5783..6211)
                     /gene="rplM"
                     /locus_tag="ACHARI_000362"
     CDS             complement(5783..6211)
                     /gene="rplM"
                     /locus_tag="ACHARI_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8441443.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6745..7212)
                     /locus_tag="ACHARI_000363"
     CDS             complement(6745..7212)
                     /locus_tag="ACHARI_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8441444.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7332..8870)
                     /locus_tag="ACHARI_000364"
     CDS             complement(7332..8870)
                     /locus_tag="ACHARI_000364"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8441445.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8950..9636
                     /locus_tag="ACHARI_000365"
     CDS             8950..9636
                     /locus_tag="ACHARI_000365"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8441446.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9837..10364)
                     /gene="msrA"
                     /locus_tag="ACHARI_000366"
     CDS             complement(9837..10364)
                     /gene="msrA"
                     /locus_tag="ACHARI_000366"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8441447.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10448..11125)
                     /gene="pgmB"
                     /locus_tag="ACHARI_000367"
     CDS             complement(10448..11125)
                     /gene="pgmB"
                     /locus_tag="ACHARI_000367"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8441448.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11122..12250)
                     /locus_tag="ACHARI_000368"
                     /pseudo
     CDS             complement(11122..12250)
                     /locus_tag="ACHARI_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12250..14433)
                     /locus_tag="ACHARI_000369"
     CDS             complement(12250..14433)
                     /locus_tag="ACHARI_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8441449.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14430..15455)
                     /locus_tag="ACHARI_000370"
     CDS             complement(14430..15455)
                     /locus_tag="ACHARI_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8441450.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15500..16327)
                     /locus_tag="ACHARI_000371"
     CDS             complement(15500..16327)
                     /locus_tag="ACHARI_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8441451.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16324..17391)
                     /locus_tag="ACHARI_000372"
     CDS             complement(16324..17391)
                     /locus_tag="ACHARI_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8441452.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17417..18253)
                     /locus_tag="ACHARI_000373"
     CDS             complement(17417..18253)
                     /locus_tag="ACHARI_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8441453.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18250..19119)
                     /locus_tag="ACHARI_000374"
     CDS             complement(18250..19119)
                     /locus_tag="ACHARI_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8441454.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19189..20472)
                     /locus_tag="ACHARI_000375"
     CDS             complement(19189..20472)
                     /locus_tag="ACHARI_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8441455.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20486..22171)
                     /locus_tag="ACHARI_000376"
     CDS             complement(20486..22171)
                     /locus_tag="ACHARI_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8441456.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22446..23471
                     /locus_tag="ACHARI_000377"
     CDS             22446..23471
                     /locus_tag="ACHARI_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8441457.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24066..27719)
                     /gene="rpoC"
                     /locus_tag="ACHARI_000378"
     CDS             complement(24066..27719)
                     /gene="rpoC"
                     /locus_tag="ACHARI_000378"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8441458.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27798..31415)
                     /gene="rpoB"
                     /locus_tag="ACHARI_000379"
     CDS             complement(27798..31415)
                     /gene="rpoB"
                     /locus_tag="ACHARI_000379"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8441459.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32143..33111
                     /locus_tag="ACHARI_000380"
     CDS             32143..33111
                     /locus_tag="ACHARI_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8441460.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33129..34034
                     /locus_tag="ACHARI_000381"
     CDS             33129..34034
                     /locus_tag="ACHARI_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441461.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34031..34837
                     /locus_tag="ACHARI_000382"
     CDS             34031..34837
                     /locus_tag="ACHARI_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441462.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            34994..35962
                     /locus_tag="ACHARI_000383"
     CDS             34994..35962
                     /locus_tag="ACHARI_000383"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8441463.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36279..36803)
                     /locus_tag="ACHARI_000384"
     CDS             complement(36279..36803)
                     /locus_tag="ACHARI_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8441464.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            37090..38844
                     /gene="scm"
                     /locus_tag="ACHARI_000385"
     CDS             37090..38844
                     /gene="scm"
                     /locus_tag="ACHARI_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8441465.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSS
                     TTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVI
                     LLVIGLRITFMSYKKSKR"
     gene            complement(38999..39985)
                     /locus_tag="ACHARI_000386"
     CDS             complement(38999..39985)
                     /locus_tag="ACHARI_000386"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8441466.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(40008..40574)
                     /locus_tag="ACHARI_000387"
     CDS             complement(40008..40574)
                     /locus_tag="ACHARI_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8441467.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(40588..41004)
                     /locus_tag="ACHARI_000388"
     CDS             complement(40588..41004)
                     /locus_tag="ACHARI_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8441468.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(41461..43395)
                     /locus_tag="ACHARI_000389"
     CDS             complement(41461..43395)
                     /locus_tag="ACHARI_000389"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8441469.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            43697..44632
                     /gene="pplA"
                     /locus_tag="ACHARI_000390"
     CDS             43697..44632
                     /gene="pplA"
                     /locus_tag="ACHARI_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8441470.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            44722..45825
                     /locus_tag="ACHARI_000391"
     CDS             44722..45825
                     /locus_tag="ACHARI_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8441471.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTYGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            45838..46791
                     /gene="menA"
                     /locus_tag="ACHARI_000392"
     CDS             45838..46791
                     /gene="menA"
                     /locus_tag="ACHARI_000392"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8441472.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFTMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(47605..48951)
                     /gene="gor"
                     /locus_tag="ACHARI_000393"
     CDS             complement(47605..48951)
                     /gene="gor"
                     /locus_tag="ACHARI_000393"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8441473.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(49085..50338)
                     /locus_tag="ACHARI_000394"
     CDS             complement(49085..50338)
                     /locus_tag="ACHARI_000394"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8441474.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(50344..51267)
                     /locus_tag="ACHARI_000395"
     CDS             complement(50344..51267)
                     /locus_tag="ACHARI_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8441475.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(51488..53980)
                     /locus_tag="ACHARI_000396"
     CDS             complement(51488..53980)
                     /locus_tag="ACHARI_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8441476.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(53987..54454)
                     /locus_tag="ACHARI_000397"
     CDS             complement(53987..54454)
                     /locus_tag="ACHARI_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8441477.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        15832 a        11499 c        10015 g        17241 t
ORIGIN      
        1 tacgggttac tgattacttg taaatcttta cgttccagtt ttattctttc taaaaaatat
       61 actcacttct tttcttagac ttcttttttt gattctcctc tttatggtct tgtccttttt
      121 tgccttcaaa atatttaaat aaaccccttt caaaagcatt tctaggtttt ggacaataag
      181 gaatgacttt tttcttcata taaatacctc atttaactat ttgatatcat cacattatca
      241 aaaaaaatag gttttttatt aaaactatct tgacctttcg ggagatttca ctgtgattta
      301 tttttattcc catacttttt tcgttggtca ctttcgtata cctaatctaa gcgatcatcg
      361 gcgacggtgc ttttacaacg aatggcagga caaaacaagg ggcggaaaaa cttttttgcc
      421 tatttcataa aaaacttttt cctacagcaa tcccttgttt tgtcctacca cacgttgtga
      481 acccaaagca cgccgacgaa cgcttagcta tacgaaaatg acctccaaaa acaaaaaagt
      541 gaggtgaata gaaacaaaaa gaaattgaaa tctcccaaaa tgtcgtccgc ctcggagaca
      601 acttgctgct gaaaatggtt ggttaattca tttaagtatt aaataagata aatcatcaaa
      661 ttaatcggaa aaatagcatg attggcctta tcaaaaataa actgtaaaaa cttatatttc
      721 tatgagtggt ggaacaatgc taataccaaa ttaatttatc gattacccac ctgcacaaac
      781 caaagatagc attattagga aacgaatcaa tcagcgaatc cagcattata atgaggatac
      841 taaaaataaa gttttcataa ttataaatcc gccttcctac gaaaggcacc aatgaggtga
      901 tgaaaacctt agagcgcttt ctttcttttt ttattccgtg ttaccctttc cttgcaggtc
      961 cctggtacac tacagaaccc gaggaggtga gtggcggggc tgtatagcgg caaccccgca
     1021 taattatatg gtgtcggtca tccgttaagg catcaatgaa tggcgcaaac ccgtagcccg
     1081 taaacttatc tcttccgaag ttgactcgat ttcgccggat gaccagtccc taccaaacct
     1141 gtactatttt tttacagaag gttgctatgt tcaaaatctt tcgtaaaaat tgttgtggct
     1201 tggatgtcca caaaacgtgg atctatgcgt gtatcggaat cacagatgcg aatggtcgta
     1261 cagtgtataa gcaagctcgc ttctcttcct ttacaaaagg tctcaatgaa ttagctgaat
     1321 ggcttgctaa atacaactgt aaggatgtgt gtttggaatc ttccggtaaa ttttggattc
     1381 ctgtttttaa tattcttgaa aaaacttgtt ttgtcacact ggctcatccg aagtacacca
     1441 aaccccagaa aggaaataaa acagatcgca aagatgcaaa aatggatttg tgatctcttt
     1501 atgtgcgaca tgatcaaacc cagttttatt cctggtcttg aaatccgcca gcttcgtgat
     1561 ctaatgcgtt atcggacgaa actgaccaac atgaaaacaa gtgaaaagaa tcgtgcttta
     1621 aactgcttaa ccgtttctaa tctgaaacta gatgatgttt ttagtgatgt gttcggaaaa
     1681 tcatccactt ctatcatcca atacttgtta gaacatcccg gtgaatcatt tgatgttcga
     1741 ccatttatcc atgcccgttg taaaactccc gttgaagaaa tccaagcggc agttgagggg
     1801 gcgatttctg tacctcaagc gattaagctt cgccaatgcc tcaaccatat taacgagatt
     1861 gagaagcatc gagaagagat caatctagag attcaagctc ttgcagaacc cttctcccct
     1921 gttttggaac tacttcgtac cataccgggc ttagacactc agccaataac agcgattgcg
     1981 attctttcag aaataggacc ggatatgtcc gtgtttccaa cttcgaaaca ctttatttct
     2041 tgggctggat gctgtccaag aaacgatcgc agtgcccgac gaacaaaatc aacacgtatc
     2101 tctcgtgcag ggcagtcgtt gaaaccgcta cttgtacaga ttgccaatgc tcttctcaga
     2161 tccaaagaac atccagaatt taaaaatcgc tatcgtagaa tcaaagcgaa tcgtggacac
     2221 aagaaagcta tcattgctat ttgtaagatg ctcctgaccg ctatctggaa tgtcttatca
     2281 aaggtggaac cctattctgc taaaggatat ctggagccaa aacctgcgaa agaagagaaa
     2341 gttctgacgc aatcccaagc tctggacctt ttaagaaaac gcggatatac cattatagat
     2401 agtgcttaaa ccaaagttac gttgattctt cctaaataga ctcgttgtct tccatttcaa
     2461 aaattttgaa aacggtaggc ttatttagtg taccgttctt caggtatctt caaatagatt
     2521 tattttcaaa cttatttcct ttcttctttt actctcctat agcttagcat ttaagtgggc
     2581 actcgtgtca ccttgctaga tagctatgaa tgccgtaata agcctcctag tatataggca
     2641 gactgccaat tataactgat tcaatccaca ttatgataaa atccaccttt tcgtttcata
     2701 tgtgttgaaa tatcacatat gtcaaaagtg cacgagtctt ttcattcagt gctttgtaaa
     2761 tactcaaaat tattcctttt cactttaaaa aaaagataat tagtacaaaa tcagaaatga
     2821 ggaaagtatt aggatggact agaaaggagt tgttgaattt gttgcaacag attggtcatt
     2881 tatcaattaa aaattaggtg aagttttatt catcactgca gctgctgttt cccagagaat
     2941 aaaacgtcta gaagaaactg gtcagttact gtattatcaa gcagcaattg actacgaaca
     3001 agcttgatta ctaaccaaag cgtttatcca cctgttgatt aaatcaaatc aaaaatcagg
     3061 tttttatacg ttcataaaag caatctccag tgtactcgaa tgtgactgta tcataggtcc
     3121 ttattcgatg ttaatcaaaa tggggtttcc ttctaccgaa tcattggaca gttttatcaa
     3181 tgatttatca aagtttggac gaaccaacac ccaaatcgtt ttctcgactc ttgtttcctt
     3241 aatggtttat cagttctgga atgtttatca tgcgctttta ttaaaataga aaagagcttt
     3301 tttcatgcga caaagcagtg atcatttatt ggcacatgca atctttgtgg atccattttt
     3361 tgaaaatacg atatttagtt ttgacccaaa aatctttaaa tacgtatttg ataatttatc
     3421 ttaacaacca gataatttta ttcagcaccg tattatctat attgaattct cggaatcaag
     3481 ataaattttt ttccgttttt gtttcttttt taatcttctt cttgttatta atagtgatta
     3541 ggatgatatt cctatattga tctttgataa ttagatataa tttttgaata acgttccctg
     3601 ctattcgagc tagaaataca agtacgccat gacctagtta gcgacaacta cttagagatt
     3661 tcttttgttc tgggacaaca aaatgccatg acaatagcag taaataatga tacttccgta
     3721 aggctcgccg taaactgggg agaggtgaaa ttcctatgtg cttacccaaa gcggttcaaa
     3781 aattttaaac tattatttta ggagatgatt ttataaaatc gaaagcctac ttgaataaaa
     3841 aacaagcctg tgaatttcta ggaattgata ataatacttt agataagtgg attcgtgtat
     3901 ataattttcc agtaataaaa attgatggcg tagttcgctt tgacgtagag gatatcaaag
     3961 atttcatgaa tacattcaaa aaaaattgaa attttcttct cgtcacatat actataaata
     4021 gcgaaatgta ctgccttact ttttaaagga gtgaggaagt ttgcctacaa agctttcaaa
     4081 tgggaaatat aaaactaatc tgaggtatcc aaaaaaattc agagaaatca ctggaattgc
     4141 atcagagaag tatcaaaaag tatttccaac taggcaatta gctattaagg ccgaaaggga
     4201 aatgaaaaag aagattgaaa cagttttaag agaggagaat gcaaactcac tagaattgaa
     4261 aggaaaaata aaatttaaag atttttatga atcaaaatgg cttcctagat atgaactagg
     4321 tcaaactact aggtcaaaca gggctccaag taatgtaaca attagtaata ctaaggatat
     4381 attccgattg cacatccttc caatgtttgg tgaatatgca atgaattatc tgaattcaaa
     4441 tactgagata atatcagatg aattaacaaa gaaatcgaaa gaatacgcaa atataaaaat
     4501 tatcaaaggc tatgttcgct ctatgtttga tattgccgaa atactatgtt atatcgaatt
     4561 caaccgaact accaaaatta ttcaatgtat taccgcgcct aagaaaatgg ctttagaaga
     4621 aaaaaggata agggaaggga atcaagcatt atcatcagaa gagctgactg attggataga
     4681 agcagtaaac aatgacttaa ataatcactt gcttacactt cacgattaca cacttttcat
     4741 gcttacctta tatctaggtg atagaaaaag tgagacttat gctctgcaat ggaaatatat
     4801 tgattttaat aaaaatacgg ttcgtctcaa atatgcttta gataaatatc aaagaaagac
     4861 acatacaaaa ggttggaaag acaccgttat tcaggcacca gaagttgtga tgacattatt
     4921 aagagaatgg aaatcagctc aggctgaaca attgttgaaa ctaaaaatta tacaaacacc
     4981 tgaccagtac ctctttacct attccaaacc ttctggagaa gtaaattgtc ctgttcacgc
     5041 tgactactta aactatcgaa tcaatgcaat aaaaaaaggc atcctgaatt ggctcaatta
     5101 tctccacaca aacttcgtca tacctatgca actatagcta gggaaggtgg agcaaatatg
     5161 aatcaaatct ctaatgcttt aacgcactcg gatatttcta ctacaaaaat atacgtgaac
     5221 acacctgacg tagttgataa atctgttttt gaagcatttc aaaaaggatt gaataagtgt
     5281 gattaacatg tgttctcaaa tgtgttctta ttataaaaaa agacactttc caaataattg
     5341 aaaagtgtcc tgaaatattg atataaaaag aatattaacg ttttgagaat tgtgaagctt
     5401 tacgagcttt tttaagacct ggttttttac gttcgaccat acgtgcgtca cgagtaagta
     5461 agccagcacg tttaagagct aaacggaagt caggatcaac ttctagcaat gcacgagcga
     5521 taccatgacg gatagctcct gattgtccag cgtatcctcc gccgtttacg ttaacaaata
     5581 cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg attgattact tcacgcaagt
     5641 cagcatgtgg aatgtattct tcaacatctt ttttatttac agtaattttg ccagttcctg
     5701 gtactagacg tacgcgagca actgcgtttt tacgacggcc tgtgccgata tattgaactt
     5761 gtgccaatga aattccctcc tattagatta agtttgtgat gtctaaaact tctggttgtt
     5821 gtgctgcatg tggatgttct gctccaccgt atacgttcaa ttttgtaaat tgtttgcggc
     5881 ctaaagtgtt tttaggaagc atacctttta cagaagtttc gatcaaacga cgagaatttt
     5941 tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc tgggtagttg ctgtgacggt
     6001 aataaatttt gtcagtcgct tttttacctg ttaatttcac tttgtctgca ttgatgacga
     6061 tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg tttattttta ccacgtagta
     6121 cagatgcaac aactgttgaa agacgtccca atggaacatc agttgcgtct actacatacc
     6181 atttacgttc tacttcgcct ggtttggcca tatatgttgt acgcacgtta ttttcctcca
     6241 atttaagttc tgatgtttga catacacttc gagtttccgg ggctctttgt ggggcaaaca
     6301 ataccattta acatattacc cgctaatcta gtcaatgtca acgtttttct gcttgattga
     6361 aaaatttata tgaaacttta cttcttttct ttcttttata taaaagggtc gtttcctgtc
     6421 aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc acaacccctt attttgaata
     6481 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccataaa acataagaag
     6541 ctgtttttta aaattgtttc ttgtgactcg gagctgaacg gcaggcttac aacctcttgg
     6601 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
     6661 actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt
     6721 ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct ggcgctttgc ccaaatgagc
     6781 ttggatcatc cagatacgtt tttctgtggc tgttttgaat tcgataaaga tatcttgtgt
     6841 tggatcgtcg ccttcttctc cagaaacttc gattcctttt tggtaaagat ctgctagata
     6901 acgatagcct gcaacaagtg tttccaaacg ttctggaata gtacgatccc agcggccaat
     6961 ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat gtagaataag gtgaaccgtc
     7021 taatgtgatc aagcgttcgg atacttcatc taactgagcg ttcaaatcat ccatgaactc
     7081 atccatcatt gggtgcaatg taaggaaacc tggtcctctc atgtaccaat gtgtttggtg
     7141 aacaacaaca gacatttggc ttagatctgc aactaattga tttaatactt ctttggtttg
     7201 ttggaatttc atctttcttg cctcctaaca ttttcttaca cttaaaataa tagtagactt
     7261 tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat tttcagttcg cttttttatc
     7321 cgtaaagcca atcatttttc agaatcagct aattcttctc gcagttgagc aaattgctga
     7381 taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg gatagatttt ctgatatttt
     7441 ttatgatcag tttcttgtac tccaacttgc tctttcaatt ctatcaattc ctgtgcttca
     7501 ctgatacttt ggatctttcc gatgcttctc cagcctaaaa tcacagctcc taatgcggat
     7561 gcttctgtga cagtagggaa aacaagcggg taacccagta catcagccat catctgacga
     7621 aaaatcggag attgagcaaa cccgcctgta gcacggatct ctgtgagctg attttcctgg
     7681 caaactcctt ttaaaatccg ataaaggttg agacagatcc cttctagaat cgccttcatc
     7741 atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac tgccaaacgc atctgcgtcc
     7801 caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa tcagaccatt ggcacccgct
     7861 ggcacaaaag cgatagccga aagcagtgca tcatatggat tttctccttt ttctttagca
     7921 gattgaacga cttcctgtaa aaatgtatgc cctgcccaat caaagatgcc agcaccattg
     7981 ctagtcgctc ctcccactac ccaatgtttc tgatccagaa cataacaaaa cgtctccatt
     8041 tctggatgga actgaggctg agtagtaata aaacgaagtg ctccgctcgt tcctacggtc
     8101 aaagcggcga tcgtatcacc aaaagcacct aaccctagat tagaaagcgg cccgtctgcc
     8161 cctcccaaaa caaatgatat atcatttggc aaacctagtt ctgctgctgt ttttgatggt
     8221 agacctgtta cttgataggt ggaaggaaag acttgcggca attgctctat tcgaatgttc
     8281 aagtagtcaa gcacttcttc tgcccattgg aattcatgga tatcaaaata tccagttcct
     8341 gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc tataaaagat atagtctttg
     8401 ataccgacaa aataagcagt ttttttgaat aattcaggtt ggctgttttg cagccacttg
     8461 attttgttca atggtgtcat tggatgaatc ggcgtcccag tctgccgata aaattctttg
     8521 ccaagcacgg tatatttcag tttttctgct tcgtccgctg ctcggttatc tgcccaagtg
     8581 atcattcgag ataacggtgt tttctcttca tccaacaata tcaggccatg catcgcagag
     8641 gagaaggaga caaattgaat gattgtgtct gaaggcaact gtttaattac ttcttttata
     8701 acggtgcaga caccttgaaa gatcaattcc tgatcttgtt ctgccatccc ttgtgtttct
     8761 tggatcaaag ggtagcgaaa ataagcggat gctgcttctt gggcttcatc tgtgaaagca
     8821 actgctttgg tttgtgtggt tccgatatct aatccgattg aaacgttcat tttttcacct
     8881 caaattttat gtatgtacaa aaatttagtt taagcttgcg tatcttggtt ttgaaaaggg
     8941 ggagatggcg tgttacttta ttttatcatt ggttgctgta tcggatcatt tttatgttta
     9001 accgcccagc ggattccgtt aggtcactct attatttatc ctagatctca ttgtgtcaaa
     9061 tgctgtcgtt cgctttcttg gtatgaattg attccgattc tctcgatcat cgtccaacga
     9121 tttcgctgcc gctattgcag gtgtcggttg cccttctact atttgttggc agaagcctta
     9181 tgtggtggtc tttttgcctg gttctttact ttttcttccg caagaaattt ttcgacgttc
     9241 atatggatgc tttcggcttt gctattttcg ttgatggatt tattttactt catggtccat
     9301 tctcacacgc tattctgttc ttgggcaatt ttatggattt tttggttgca ggccggcgtc
     9361 ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg gtgtgtgcct tcgctatggt
     9421 caatcctatc tcggagctgg cgatactctg ttgatgcttt cttggagtgg tggcttgtct
     9481 ctagaagagt tgcttcagat cttgtttctt gctagtctgt ttggaatatc cgttttttct
     9541 gtttacttta tctgtcataa aaaaaagttg gaaaagctgc ctttcattcc ttttttgagc
     9601 agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa aggttgtgac aaacgacttg
     9661 tcacaacctc tgatatatag tgttctctta ccaaatcctt gtgacttgga gctaggcgac
     9721 aggttcacaa cctctccact aaatggtgtt caatcagctg accctcagta ttagctcaaa
     9781 atgctggttt tacaacctct gatatacggt gttccataag tagctttttc ggcttcttat
     9841 ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc cagaaccttc tcgataaaga
     9901 ttgtaatgga gcttgttttt cttgtaataa tcctgatggt aatcttctgc aggataaaat
     9961 tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga atcgcccgct gtcttgcaat
    10021 gcttgttttg aacgctctgc aatatttcgt tcttcttcat cattatagta gatcactgga
    10081 cgataagaat caccgcgatc agcgaactgt cctaatgcgt cagtcggatc ggtttgctgc
    10141 caatagattt ccaccaactg atcataagaa ataacttctg gatcatattc gatctctact
    10201 gcttctgtat gccctgttgt gcctgttgtt acttgttcat aagttggatt tggtacatgc
    10261 ccgccagtat aaccggatgt cacagaaatg atccctggct gctcttcaaa tggtttgacc
    10321 atacaccaaa agcatccgcc tgcgaaaact gctttttttg tcatataaaa tccccctttt
    10381 ttctcatcat accttgattt ccaaaaggag gataacgttt tgcttgtgac agaaaaaacg
    10441 gaaactatta tatacgtgat acggcttctg ataaatgcca aaacgaacgt acttgatcaa
    10501 aaacggttaa atcaggaatc acatattcag cagaaggaag atttatgttt tcacctattc
    10561 ccaatacatg catgttacca gccaaagctg cttgacaacc agcttctgaa tcttcaatga
    10621 ccaagcatgc atttggcggt acattcaatt gctgtgctcc tttgagaaaa acttctggat
    10681 caggttttgc ttttgatact tgcgtgccgt caataaggac atcaaaataa cttgtcaaat
    10741 tcgttttttc taaaatgagg cgtgcatttt tgcttgctga tcccaatccg atcttgatgt
    10801 ttgttttttt caagtactca agaacagcta ttgcaccagg caaaacggat gtttcatcca
    10861 ttttttgaat cgcttgaaga tatagattgt ttttttgctc agcaaaggct gatttctctt
    10921 tttctgagaa tttctgttcc atttttccaa atgacagcaa tcgctctagt gactcggttc
    10981 tggatattcc tttcagttgc tcgttttctt tctcagtgaa tggaatggac agctggttag
    11041 ctaaaaccag ccacgcttcg taatgataac gagctgtgtc tacaagaacg ccatctaaat
    11101 cgaatagtgc tgctttgatt ttcatcgatt acctccttga aaatgaacga cttgaagtaa
    11161 agatgcgatg tttttagctg tgcgatgaag attatcagtg gcttgctgta aagcttcttg
    11221 taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa atgccaacat ctgctgcttt
    11281 ttcgcttcct ttaccaacac ttccgcaaat agctatcaca ggaacgccct ctggtactct
    11341 tctagcaatc ccaactggcg cttttccttt tagactttgt ccatctagac gtccttcgcc
    11401 tacgattacg aggtctgctt gctgtacccg tatcgaactc cagtaaatcc aatacgtagt
    11461 cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac
    11521 cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca
    11581 tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa
    11641 aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg
    11701 ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt
    11761 ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg
    11821 ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa
    11881 caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg
    11941 gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg
    12001 cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga
    12061 ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc
    12121 catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt
    12181 gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca
    12241 gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc
    12301 tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt
    12361 tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca
    12421 tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa
    12481 accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga
    12541 tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca
    12601 ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc
    12661 gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata
    12721 ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt
    12781 tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt
    12841 ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt
    12901 gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg
    12961 atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata
    13021 tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa
    13081 atgatcatcg acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt
    13141 ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc
    13201 ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt
    13261 tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct
    13321 ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc
    13381 taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa
    13441 gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt
    13501 tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg
    13561 gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg
    13621 atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact
    13681 aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt
    13741 agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa
    13801 ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc
    13861 aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc
    13921 aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc
    13981 gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag
    14041 ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt
    14101 tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc
    14161 ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat
    14221 ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg
    14281 gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct
    14341 tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata
    14401 ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc
    14461 gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc
    14521 atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg
    14581 tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt
    14641 tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct
    14701 tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc
    14761 agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc
    14821 cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta
    14881 ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc
    14941 ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga
    15001 agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt
    15061 gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg
    15121 gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc
    15181 actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg attggctgta
    15241 cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca
    15301 tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca
    15361 gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct
    15421 acttgtccag cgcctatgat tgctactttc ttcatttatt tctctctcct ttgcttattt
    15481 acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca
    15541 gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca
    15601 aacgacattc atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc
    15661 gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat
    15721 gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca
    15781 tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac
    15841 gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa
    15901 taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt
    15961 tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc
    16021 cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct
    16081 cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat
    16141 cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg
    16201 tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt
    16261 tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga
    16321 atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg
    16381 gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat
    16441 ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt
    16501 acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt
    16561 tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa
    16621 ggcaacataa gaaatgatcc cgccatagcc gatccctctt aaagaagctt gtaatgcggc
    16681 agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag
    16741 acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt atttttctgc
    16801 taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt
    16861 agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc
    16921 tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac
    16981 tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt
    17041 tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc
    17101 tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc
    17161 gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc
    17221 tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg
    17281 ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc
    17341 gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt
    17401 cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc
    17461 gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca
    17521 ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa
    17581 atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa
    17641 agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa
    17701 tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg
    17761 tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga
    17821 taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa
    17881 caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc
    17941 gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag
    18001 ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa
    18061 tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt
    18121 gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg
    18181 gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct
    18241 ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat
    18301 gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt
    18361 ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa
    18421 catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc
    18481 cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc
    18541 ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata
    18601 gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca
    18661 cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata
    18721 actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact
    18781 taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc
    18841 tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa
    18901 acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat
    18961 aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga
    19021 aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat
    19081 ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc
    19141 tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt
    19201 tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac
    19261 tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc
    19321 gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc
    19381 aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt gtttattcac
    19441 tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa
    19501 gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt
    19561 caatccagat actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat
    19621 attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat
    19681 tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat
    19741 cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat
    19801 cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa
    19861 cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc
    19921 tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa
    19981 accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg
    20041 tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata
    20101 attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc
    20161 tttatccatc actttagcaa aatcctgact gacttccatt acttctggta atttaccgga
    20221 acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat
    20281 tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg
    20341 ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct
    20401 ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat
    20461 aatttttttc atttttttcc acctcttatt ttattatttt cagccacata tattcatacg
    20521 gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta
    20581 ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta
    20641 agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta
    20701 agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg
    20761 tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg
    20821 ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt
    20881 aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta
    20941 ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag
    21001 aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt
    21061 ttttcttgta gtttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg
    21121 ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa
    21181 aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact
    21241 tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga
    21301 ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat
    21361 ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt
    21421 ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa
    21481 atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat
    21541 cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa
    21601 aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc
    21661 tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat
    21721 ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat
    21781 gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc
    21841 atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg
    21901 tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat
    21961 aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag
    22021 taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct
    22081 gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc
    22141 gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt
    22201 cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat
    22261 tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta
    22321 aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa
    22381 aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata
    22441 taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg
    22501 tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc
    22561 aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg
    22621 gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt
    22681 ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga
    22741 ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag
    22801 aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg
    22861 aattggatca atacaatatt cctattgtgg taatcggaaa agtcggaaac gactacacga
    22921 acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga
    22981 tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca
    23041 atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg
    23101 aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa
    23161 tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta
    23221 tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat
    23281 tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga
    23341 caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc
    23401 ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac
    23461 aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg
    23521 ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt
    23581 attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt
    23641 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
    23701 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
    23761 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
    23821 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
    23881 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
    23941 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
    24001 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
    24061 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
    24121 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
    24181 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
    24241 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
    24301 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
    24361 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
    24421 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
    24481 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
    24541 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
    24601 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
    24661 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
    24721 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
    24781 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
    24841 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
    24901 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
    24961 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
    25021 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
    25081 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
    25141 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
    25201 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
    25261 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
    25321 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
    25381 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
    25441 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
    25501 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
    25561 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
    25621 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
    25681 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
    25741 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
    25801 agcatcttag aagtttcttt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
    25861 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
    25921 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
    25981 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
    26041 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
    26101 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
    26161 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
    26221 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
    26281 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
    26341 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
    26401 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
    26461 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
    26521 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
    26581 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
    26641 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
    26701 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
    26761 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
    26821 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
    26881 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
    26941 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
    27001 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
    27061 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
    27121 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
    27181 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
    27241 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
    27301 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
    27361 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
    27421 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
    27481 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
    27541 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt
    27601 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
    27661 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
    27721 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
    27781 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
    27841 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
    27901 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
    27961 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
    28021 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
    28081 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
    28141 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
    28201 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
    28261 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
    28321 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
    28381 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
    28441 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
    28501 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
    28561 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
    28621 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
    28681 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
    28741 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
    28801 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
    28861 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
    28921 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
    28981 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
    29041 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
    29101 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
    29161 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
    29221 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
    29281 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
    29341 gaagcatcaa cgtattcaac gacaccatca tgtttacata acaaagcagc tcctgagtca
    29401 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
    29461 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
    29521 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
    29581 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
    29641 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
    29701 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
    29761 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
    29821 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
    29881 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
    29941 gcgaaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
    30001 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
    30061 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
    30121 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
    30181 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
    30241 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
    30301 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
    30361 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
    30421 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
    30481 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
    30541 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
    30601 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
    30661 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
    30721 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
    30781 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
    30841 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
    30901 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
    30961 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
    31021 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
    31081 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
    31141 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
    31201 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
    31261 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
    31321 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
    31381 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
    31441 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
    31501 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
    31561 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
    31621 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
    31681 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
    31741 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
    31801 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
    31861 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
    31921 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
    31981 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
    32041 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
    32101 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
    32161 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
    32221 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
    32281 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
    32341 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
    32401 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
    32461 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
    32521 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
    32581 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
    32641 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
    32701 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
    32761 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
    32821 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
    32881 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
    32941 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
    33001 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
    33061 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
    33121 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
    33181 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
    33241 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
    33301 atcatccatt gacagcttgt cttctaggct ttttagggag tgcggcagca gggcttgttt
    33361 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
    33421 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
    33481 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
    33541 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
    33601 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    33661 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    33721 ctggctcctt gcttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    33781 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    33841 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    33901 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    33961 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    34021 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    34081 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    34141 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    34201 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    34261 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    34321 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    34381 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    34441 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    34501 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    34561 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    34621 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    34681 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    34741 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    34801 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    34861 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    34921 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    34981 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    35041 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    35101 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    35161 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    35221 tcctgatttg aaagtacaaa cgatgatgac ttctgaatct acgatgaaag atgccaaact
    35281 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    35341 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    35401 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    35461 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    35521 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    35581 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    35641 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    35701 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    35761 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    35821 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    35881 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    35941 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    36001 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    36061 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    36121 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    36181 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    36241 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    36301 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    36361 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    36421 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    36481 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    36541 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    36601 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    36661 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    36721 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    36781 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    36841 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    36901 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    36961 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    37021 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    37081 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    37141 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    37201 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    37261 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    37321 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    37381 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    37441 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    37501 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    37561 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    37621 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    37681 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    37741 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    37801 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    37861 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    37921 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    37981 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    38041 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    38101 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atcaacttca
    38161 agcgacagta cagatactac ctctaccaca agtgatagca cggatacatc agcttcaagc
    38221 gacagtacag atactacctc taccacaagt gatagcacgg acacatcagc ttcaagtgac
    38281 agtacagata ctacctctac cacaagtgac agcacggaca cgtcagcttc aagcgacagt
    38341 acagatacta cctctaccac aagtgatagc acggacacat cagcttcaag tgacagtaca
    38401 gatactacct ctaccacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
    38461 actacctcta ccacaagtga tagctcagat tcatctacca ctacaagtga tagcacggac
    38521 acgtcagctt caagtgacag tacagataca tcctctacca caagtgatag ctcagattca
    38581 tcaacttcaa gcgatagtac agacacgtcg tctaccacaa gcgatacacg tcatgcaagt
    38641 gtctccttct caaaaacaag ttcggataac aggacgaact ttaacttaac taataaaaca
    38701 agcaatgaca atggaaacaa atcaaaaaga gcacttccta aaactggaag tcaatcgaat
    38761 aattggataa cactcgcagg cgttatatta cttgtaatcg gtttaagaat cacttttatg
    38821 agttataaga aatcgaaaag gtaaaaaaat attcaagata aaaaagagca acgtcgctaa
    38881 cggacgttgc tctttatttt tgctttttat cataaataca agaactacaa ttcaatttgt
    38941 caaaaaacac gtagatttcc ttctgagaaa tttatttacg tatcgaacct ttccctattc
    39001 agtctttgcg atccaaaatc agttgggtaa gggaaaagag attctctttt gttcgaagag
    39061 aggtggcagg taatttcttt atttccttca aagccttttc tgtgtaagag gccgctaact
    39121 tcctagcttc ttccacgcct cctgtatgtt caacgatttt gcgtacggcg tcgacatctt
    39181 cttctgtcat tgccgctttc ttctctaaat aaggtagtag ctttggtttg tttttttgca
    39241 aagagtagat caacggtaaa gaatagaccc cttgacgcat gtcttctagt acaggtttgc
    39301 cgatttcctg gctctctttt gtataatcta aaatatcatc gataatctga aaggccatcc
    39361 cgatagaaag tccgatatct cctgcttttt ttgcgaagcg ctcgcttgtt ccactttcaa
    39421 aagcgccaac agaacaactt aattggaaca gttcagctgt cttacctgaa atattttcca
    39481 aatattcttt tacagaaagt tcataattat agcgatcatc catctgccct aattcgccat
    39541 tcaatacttt ttccatactg cgagaattca attggagact tttcaaggaa gtggagtaat
    39601 cagacaatag tttaaaacag cagacaaata aatagtctcc tgcatagaca gaaacgacat
    39661 taccaaactg actacggata ttaggctgac cacgtcgcag atccgcttca tcaacgatat
    39721 catcatggat cagtgtggcc gtatggagca tttcgataga agctgccaaa gcgatggctt
    39781 tgtctcgatc ttgctctggt ccaaattgtg agaacaatag ctgataggca ggacgcaaca
    39841 gctttccgcc ggcatggatc gtttctttga ttgcttgttc gacaggtttg tttttcaggc
    39901 ggacagtttc ttccatccgt tttagtgttt ctgtcaattc tttacttaaa gcaggatagt
    39961 tttcccacat tggatgtagc ttcataaaaa aactccttgt gtgacgatta ttttttggta
    40021 aacattgggc tccatttttg tttggttccc tgctcataat ctttttggaa tcgttgaagt
    40081 gtctgcacat gtcgtaaaag ataattcgct gcgatcccga ttgcaatccc tgataaaatc
    40141 cctaaaactg aaaggactgg caagtacaac atgactgtcc atgattgtgc aatgaaagaa
    40201 gccatcaaaa gctgtccgac attatgcatg aaaccgccgg ccgcactaat tccgatgata
    40261 ctgacacgtt ttggtcctaa ttgcttgacc aataacattc caaaataact aagcaaagca
    40321 ccactcatac tataaaggaa tgtggaaatc gttccgccta gcaaagtagt caggaatagt
    40381 cgtagccaaa gtagtaagaa gctgtcttgt tttttcatgg taaaaatggc aacgatcgtg
    40441 attagattgg ctaagcctag ttttgcacca ggcgcaaaag aaaaaggata agggatcata
    40501 ttttccaata aaccaatgat cactccttgt gcaacaagta gcgaaatata aatcattttt
    40561 tgtaatttac tcatggtgtg taaaggtcta ataaatcaga ctgtcttcgt cacctccatc
    40621 tgttgaacgg acttcgatca ctaatttatg tggcaaacag acgatggttt cgccattttt
    40681 cgttgcccag cctcgacgta cgcagatctg gtcatcgcaa tctgcttctt ttatccgtac
    40741 tcgatcgcca tctatctcga tcaagttata atctccgtgt tcatctttgt aagtatatgt
    40801 gttgctctct tctccagcca tcaaagggaa tgttttgatt tctgtgccat ccacacgcag
    40861 taccgcttct ttttctgctg ttgcggtgtt ttgctgaaga ccaaaaatca tgactggtaa
    40921 aaaagaaaga acagtcaaaa gcaaaataat aaaaacatcc caaggtttga gcagactttt
    40981 ttcagcaaat tctttaaggc tcatttttgt tctccttttt ccagattaac tcacccaatt
    41041 ttagcataaa aacaagaggt tgtgacacct gtgtcttgct ttgagtaata agaacgaaga
    41101 atagaaaaca gctttccatg ttttctaatt ctttgggctt attaccgaga agcagcaggt
    41161 ggaacaccgt ttataacaag aggttgtgac agaagtgggc aacttcaaga aataagaagg
    41221 aatttacgaa aattactttt caagtttttg tgaaatttcg gcttattttt cgaagaagct
    41281 acttctggaa caccgtttat aacaagaggt tgtaacagag gttgtgacac ctgtgtcttg
    41341 ctcatggagg cttatctcgg gcatcttatt cctttaattt tacataaaaa ggaggctgtg
    41401 acggtttgtc tcagcccctt tctattgaaa tcttgcattc aaataacgtt ctttatctat
    41461 ttatttcttg ccgtactggg aacttggtct accgtaccac caattaccta gtgtacgttg
    41521 tagccatgga attacccagc gatccaaccc aatcgcacgt ccggaaccgt tcatcaatgc
    41581 gaaagcaaca aaaatgaacc aaatgtttac ccagtaaaac atgcctgata agcagaacgt
    41641 gatcaccaat acaatcgttg tcgcactgca tagccatgtg aataatccag caatcaatgc
    41701 taatgccaag gcaatttcaa agaataccat gaatttttgg aagaacattg ctacatcatt
    41761 gtttggcatg atcacttgca tgatctttgt gaaccagtct ggtgacttct caatcacagc
    41821 cattggttct tcaccgtatg cataactcaa accaaaatgt gcagcttgag tcgctgcttc
    41881 tccggcactt gcccctgctc cgctggcagc tgtggttgca tcaccagcag cagaagcacc
    41941 actggcaaca tctgttacat tgtattccca tgtccaaggg aacacactgt ttccagtgaa
    42001 ccatgatcct tcaccaaacc aagtacttgg tttgaacact tcaccattcc caatgatttt
    42061 tttggaagct tcaacgaacc aaactaaacc atagaaaata cgaagtggca cactccataa
    42121 gacattgccg taacgagaag aatgtcctcg agtcacgttg cgatcatctt tgatatggaa
    42181 aaactcatgc attaaatatt ggaacatata ataaccagaa cgaatatcaa agaagtattt
    42241 caaattcacg atatgtttca ttacgatagc taaaaatccg cttaaatgga tcttatcaaa
    42301 taggttagct accccccatt tggcaccaat agaaaccata aatccttgat agtttccttt
    42361 aaatttatgc ttttctccgc ctttgatcga agccacgata ttcgctgcgg ctgtgtgtcc
    42421 tgtttgttca gcagcttgaa cgatctgcgg tgtcggtgtt cctgggaatt cttcataaaa
    42481 gactaaatca ccaatgatat agatgttttt atcttcgtag ccttttgcct gcatatattc
    42541 attggcgatc aaccgctgtc cgcgtgctga ttctaatcca aagtcagcag cgtcagaagt
    42601 tcctttgaca ccagctgtcc aaatcagtgt gtgagtcgga atgttgcgtc cgtctttcaa
    42661 tttgatatga tcagcagcca cttctacgat tggtgcattc aataataatt ccacgttgtg
    42721 cttacgcaaa tagcgttcag cttttgcagc atcatttcga tccaacatat tcaagatcgt
    42781 cggcattgcc tctacgacca tcagtgtgat ttcgtttgga tctaatttat aatctttggc
    42841 taggcgattt ttccagtcga tcagttcccc aatcatttca ataccggtaa atccagaacc
    42901 acagacaacg aaagtcagca tggctttacg cagttcatca tctggttcca atgcagcttt
    42961 tgctactgtc ttctcaatat gttcccgaat ccttacagaa tcttcaaatg accaaagggt
    43021 gaatccgtgt gctttgacac caggtgtacc aaagtcattc ggctctccgc ccattccaag
    43081 gatcagctga tcaaaaggat aggaacctaa tttggtcttg acgatttttc gttctttatc
    43141 gataccgatt acagtgtctg tgacaagttg tacattcttt tgacgagcaa acaaatgctg
    43201 cagatcgtat tggattgctt ctggttctac acgtccgcca gctacttcat gcaactcggt
    43261 catcatagta tggtacgaat gacgatcgat cagagtaatt gctacatctt cttttttcag
    43321 tttctttgcc aagtattttg tagctgaaac accggcatat cctgctccta caacgacaat
    43381 attttgcttt gccattgatc atccgctcct tatagactat ctatataatg aatatatgat
    43441 aattctttta gtttgatact tattacacaa tctccatata ttatatagat gttctgttct
    43501 tttgtctaac tccttttgga tttttttcaa attttaaaat aaagcgtttt ttcacgaaat
    43561 ttgctgaaag aaaacgtttg aaatattaca tgggttattt atcaaaaata gttgacttat
    43621 aggctaaaaa gattagaatg tataatgaat cacaaagaaa aattgtggac tagaaatgga
    43681 atggtggggt attaaaatga agaaaaaacg ttttgtaact ggatttgccg tattagcatt
    43741 ttcagctcta gtgttaggcg cttgcggcgc agatgacaac ggaagctcaa actcttcttc
    43801 tgaatcatct acagcacaat cttcgactgc aaaaagcaca acagaatcat ctgcaaaagt
    43861 tgtagctggc ggagatttgc aagacggcac atataaatta gaagaaaaaa attattcaaa
    43921 tggttaccgt gcagtctttg aaatggttgt aaaagacggc aaaatcactg aatcaaaata
    43981 tgataacgtc aatgaaaacg gcgaatcaaa aacaaaagat gcagaataca acaaaaacat
    44041 ggaagcaaaa tctggcacaa acccagaaaa atacattcct gagttgaatg accaattctt
    44101 aaaagcacaa agcgcaagcg gtgtggaagt cgtaactgga gctactcact catctgaaag
    44161 cttccaaaac tacgcacaac aattgatcca agctgctcaa gctggtaaca cagatacaat
    44221 cgaaatcgac aatggtgctg atttgaaaga cggcacttac aagcttgaag aaaagaacta
    44281 ctctaacggc taccgtgtgc aattcgaaat gacagttgct ggcggcaaag taactgaatc
    44341 aaactttgat tatatcgaca aagacggtaa atcaaaacaa gatgatacag aatataatga
    44401 aaatatgaaa gcaaaaagcg gtacagaacc aaaaacttac atccctactc tgaacgacga
    44461 attcgtcaaa gcaatgggcg aagaagatgg ctcacctgct aatgtagaag ttgttacagg
    44521 tgctacacac agctcacatt cattcatcat gtacgcacaa caattagtga atgcagctga
    44581 aaaaggcgac actcaaacaa tcgaagtaga taatatcgta acagaaaaat aatcacataa
    44641 tcatactaat aaaagttgga gggtgggacg atagccagat taggcagtcg tctcaccttt
    44701 caaaatgaag agaggaaaaa gatgcggaaa aaactactat tttccttaat cacactgggc
    44761 atctctttta tggtgctaac tggctgcagc aactcagcaa ataaaacaac cactgaaagt
    44821 tcagcagcta ccaagatatt gaaagatcca tattctgatg aacagttctt gttagggact
    44881 tacgttcgta tccgtgtgta tgatgaaggc aaagaatcag cattgaaacc tgccttcgat
    44941 cgagtaaaag aacttggtga taaaatcacg atcaatcaaa aaggttctga gatcgatgaa
    45001 gtgaatgagc aagctgggat caaaccagta aaagtttctg atgacgtcta cacgttagtc
    45061 aagcgggcct acgaatatag ccaagattct caaggcggat tcgatatggc gattggtgca
    45121 atcacacaat tatggcgaat cggttttgat gacgccagaa aaccttccca agaagaaatc
    45181 gatcaggcat tgaaattagt ggattatcat aagatcgaac taaatgacaa agaaaaaact
    45241 gtttatctaa aagaaaaagg aatgatcatc gatttaggtg caatcgccaa aggttacatt
    45301 acggacgaag tagtgaaagt cttgaaaaaa caaggagtga ccactgccat cgttgacttg
    45361 ggcggtaatg tctatgtctt aggccacagc ccaagaggcg aaaaccaaga ctggacagtc
    45421 ggcatccaag atccgaacat ggctcgcgga tctgttttag gttctattaa agaacggaac
    45481 aaaacattag ttacctatgg aatttacgag cgttatttag aagtagatgg caaaaaatac
    45541 catcatttgt ttgatccaaa aacgggttat ccgttcgaca atgatatcgc aagtgtcacg
    45601 atcataacag ataaatcaat tgatggtgac gggttgtcta cagcagtctt ttcaatgggt
    45661 gtgaaaaaag gactggaata cgtcgaaagc gaattgaata acgggacgga agcgatcttc
    45721 gtgacaaaag acgataaagt atatgtgacc gatccaatca aagacacctt caaattatcg
    45781 gaggactccc attacacgat gggtgaccgt tctgaactaa aataaggagt acgaaccatg
    45841 acactaaaaa tctttttaga agtagttgaa atacgaacaa aggtggctag tgtatttcct
    45901 tttgcggtag gggtattatt ctctatcgct ttttttcatg agatacattg gctaaacact
    45961 tttttatttt ttattggaat gctagttttt gatttagcga ctaccgctat caataattac
    46021 atggattatg aaaaggctca ttctgaagta tataagtatg aagaaaatgt cattggccgc
    46081 agaaatctat ctcctgtatt gattcgaaac atgattttag ctatgattgc atttgttttt
    46141 gttatcggcg ttattctaac gatcaatacg ggttggcttt tacttttgat gggatttatc
    46201 tgttgtttta ttggagtatt ctatacattc ggaccgattc ctttatctcg aatgccttta
    46261 ggggaaattt tcagtggggt gactatgggc cttggaattt ttaccatggt cgtttatgtc
    46321 aatacattcg gttcagatgt ctatgatctg acagtcaact tttcatctgg acaatttgct
    46381 ttggtaggaa atctttgggc tatagcagca atcgtattag cttccttacc cttggtgttt
    46441 acgattgcca atatcatgtt ggctaataat ttgcgggatc ttgaccgtga tattgaaaat
    46501 catcgctata cactagtttt ttatatcgga cgtcctgcgg gaacagtctt attccagcta
    46561 ttgatgtatg cttgctattt cgtgatttta atggggttgt tgagcggtat ctatgaatgg
    46621 ccgatcttgg tcaccttctt gaccttgcca attgtgaaga agcatttgga tcaattcaag
    46681 caaacgttac cccagcctat cagtttcagc tattccatca aaaatatggt cttattcaat
    46741 gccagttatg tattggggct attactaaca atcattatgc aagccattta acagcatgaa
    46801 gaggttgtga aaaaactgcc ctgcatcaag taataagaac agccaaataa aaatagctcc
    46861 ttatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttat
    46921 caggaggttg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatggc
    46981 tttccacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
    47041 atcagaggtt gtaaaatcag tgttttgtcc tgagtattag agaaacaaat ggaaaaatag
    47101 ttcctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg aacactattt
    47161 gttcggaaaa agtgactaac tatgacacaa tttttttcac agtccctttt ttcacttgat
    47221 ttgattcgta ccagcgaact tgacgagaac tttcttggtt tggtaactat atggtattct
    47281 tttaacatcc tcgtttgctt cttctaattt atttatacaa agtcacactt ttttaaacat
    47341 ctgttcttcg atacatttct atttctttta aacatcgata ccaatatttt gaacttaaga
    47401 gtgcatacca aatggattca tttactcttc gtcagttacg aagataatca aaatctccaa
    47461 cttccctact tatttcgttt tgagaattat cgaagcaatc ttacataaat aactaaaaaa
    47521 caaaaaacca gaaaatcagt atactgattt tctggtttta catggtttgt tcaataaaaa
    47581 gctgttggaa ctgttaatat taaattatcg catcgttacg aattcttccg ctcctgttgg
    47641 atggatggct actgtattgt caaagtcttc tttagttgca cccatcttga tagccacggc
    47701 aaaaccttgc agcatctcat cgacaccaac accaatagca tgtaagccga cgattctttc
    47761 ttcttttccg acacagatca atttcatttc gcatttttgg cgataatcat ttaatgcaaa
    47821 gtacattggg gtaaagcgtg aacgataaac tttcaattca ttttcgccat atttttccaa
    47881 tgcttcttct tctgtcaaac caattgttgc aattggtgga tgcgtaaaga cgactgtcgg
    47941 aacaagttta tagtcaagat aagaatcttt ctttccatta aataaacgtt ctgataaacg
    48001 tctaccagct gcgattgcaa caggagtcag atctaattta ccgatcacat caccaacagc
    48061 aaaaatatgt tcatccgttg tattttggta tttatctact ttgataaacc cttttttgtc
    48121 caattccaca tttgtatttt ccagacctaa tttttcggta ttcggtacac gtccaccggc
    48181 aaaaagtaca caatcaccag ttaaggtttc accattttca aatagcaccg tatattcgtc
    48241 attgttttta gtaatttctt tagcaatatg atgtgcgtaa gtttttattc cagcttcttg
    48301 gtacatttgg atcaaattgt ccgaaagcat tttatcaaac gttctaagtg gtcgttcttt
    48361 tcgatatgcc cacatgactt cactacctaa accacgaaat acacctgaaa gttcagccgc
    48421 aatgtatcca gcacctaaaa cgattgctct cttaggtaac tctgttaatt caaaaaatcc
    48481 atttgaatca attgcgtatt ctccaccagg aatggtcatt ttacttgcac ggcctccagt
    48541 agcgatcaag attttaggag cacgataggt ttcaccgttt acttcgatcg tttgactatc
    48601 gataaaagta gcgtaacctt ttatagcctc gacatgattg ctatctaatc ctctttgata
    48661 agcaccgtgt agaaaatcaa tatatttttc tcgtctttct actagaccgg cgaaatcaac
    48721 attttttaaa tctgcttgga taccgtaact tgaggcatct cgttcaattg tttccaaaat
    48781 cgtacttgct tgccacataa cttttttagg tacacaaccg acgttgacac atgttccgcc
    48841 caattcattc gcttcaatca gcaaaacttt tgcgccatgc atccctgctc tgtttgctga
    48901 agcaataccg ccagagccgc caccaattac gatgtaatca tatatttgca ttttgttcct
    48961 ccttattgaa gagatcaatc aaaaccttta tttaacgatc ctttttctac agaaaacaag
    49021 tgtaacactt ttcttgaatt ttgtatgcga agatgcttaa aacagggata tatcagcttt
    49081 ttccttattt acgttttcgg atcatatctc caggtttcac cggctgtgcg actggcatag
    49141 ttaaaatcat cattggattt ggtgctcggt caatggattc gccttcttca ttatgcataa
    49201 cttcaacggt ttgactgaaa tgactgaatc ctggaccata aaattcgatt tcatctccta
    49261 cagaaaacaa gtttctctga cgtatcgtag caatctgtgt tgcctcatca taagataata
    49321 cttcaccgac aaatttatat actgggattt tacgacgttc accgaataat tgttcgtttt
    49381 cagttggtgt attgtagtaa aaacctgtag ctaattctcg ttgtgctact ttccataact
    49441 cgtcgatcca ttcttgcttg cagacatagt tttcaggatc ttccatataa ctgtctacag
    49501 ccgctttata gacatttgac accgtagata cataatggat cgatttcatt cttccttcga
    49561 tcttgaagct gtctacgccg ttttcaatca attctggaat atgttcgatc attgacatat
    49621 ccactgcact catcgagaat tcttcttcca cttcgcctgt atcagttaaa ctgtttcgtt
    49681 cttgaccaaa aggcatatcg aacaatccat acttccagcg acaagattga gaacatccgc
    49741 cacggtttgc atcccgcatc gacatatggt tggacaatgt gcatcgacca gaataagaga
    49801 tacacatggc accatggata aatgcttcga tttcggtatc cgtattttta cgaatctctg
    49861 ccacttcctc cattgaaact tcacgagcta aaacgacacg ttccaaccct tcttctttcc
    49921 agaattccaa tgtctcaaaa ttagttgctg aagcttgagt ggataaatgg atcggcagac
    49981 ctggggcttc tgtcgcacaa atttcgatca aagctggatc agaaacaatc actgcagaaa
    50041 taccgacatc acgcaaactg cggaaaaatt cccctgctcc atcttcattt ccttcatggg
    50101 tgaccatatt agcagcaacg tataccttcg catcatgttc ttttgcaaaa gcaactcctt
    50161 cagccatttc ttcataagta aaattacctg cacggcttct taaaccatat gcattcccgc
    50221 caatatatac tgcatctgct ccataatgga tcgcggtttt taacttttcc agtgttcctg
    50281 ctggcgctag gatttctggt cgtttcaatt ttctttttac tgtcatttct ttcatcatac
    50341 tccttatttg atagcagaag gatcaatata aaagaatcct gtatctaatt cacggttttc
    50401 aggatgcaat ttactgattt cgctagtcat tttttccgct ttttcttgat cccagctacc
    50461 ttctaaaatt tgtttttttg cttcggcgaa tagtcgagtg atgtttacga atttttcgcc
    50521 tggagtaaat aatccgtcca atttccatgt acagtaatga tgctgataaa gttcattcaa
    50581 ttcattcgag agattcagat cattgctggc aaaaatatgt gtgccatgac tatcttcata
    50641 aatagaataa tgtgtctctt cttttttagg ttccgagata aaaagacctc tttcacgatc
    50701 tttttgttcg tcttgtttcg tatagttata gtaattctgt agcagtggac gtttggattg
    50761 atggatgcat gtagcaccat acacaagggt ttctacagga atatcgagtt tttcttccat
    50821 cgccttcatc tcttcaaaag gaacttcacg agcaagtact gctccaactg cgcctttttt
    50881 cgcccagaaa ttgatttgac gtgcgctcgt tactaatgtt tcagcatcat aaatatacgg
    50941 tatacgcaat tcaggatttt ttttcatcgt ataaataata cctgtatcac caactgtgat
    51001 cacatctacg tttatttctt ttaaaaaagc caaatattca ggtacatttt tcattttttc
    51061 aggatgaatg atcccattta cagcaatatt cgctttttta ccatacttgt gtgctaattc
    51121 taccaattgt cgctgttctt ctcgtgtaaa tgaagcaggc agacgcaatc caaattgctc
    51181 ttcaccgaaa tatagtgtat ctgcacctgc agcaagcaac tgttctgctt gttcaatagt
    51241 ttctactgtg gttatgagtt cgatcataat tttctcctcc tacaacttca taatagtagc
    51301 atagctctaa aaactttcca atacttcttc tatttaaagt cggatttttg aaaattattt
    51361 cacaaaaaat caaaataaag ttcgatatat tctgtccaaa tataaaaaga gattgtgaca
    51421 aaaatcttgt cacaacctct taatcaagtc ttactattat aaaaattcag ctacacaggc
    51481 caagtaacta tagggaacct actgtttcct ttttaggtcc gcgaacattc aatgtgatct
    51541 taccttttga agcacctact gttacgtggt cacctaaacg aatttctccg ccaagcaaag
    51601 cctcgctcaa tcgatcttca atttcttttt gaagtgcgcg acggattgga cgtgcaccat
    51661 attcaggatc aaatccagct ttcccgatca catcgattgc agatggtgtg attttcactt
    51721 gaatatcttg ttctttcaat cgtttgataa tcgctttgct catgatttgg acgattgtat
    51781 ggatttcatc tttactcaat gaatggaaga cgacagtctc atcgattcga ttcaagaatt
    51841 caggtcggaa agctttcttt aactcttcta agatacgttt ttgcatggct ttgtgatctt
    51901 tagtaacatc ttggacattg aatccgacat ttttttcttc tcgaatttgt gtcgctccga
    51961 tatttgaagt cataatcatg attgtatttc taaagtccac ttttctacct ttagaatctg
    52021 ttaaatgacc atcatccaat acttgcagaa gtaaattgaa aacgtctgga tgtgctttct
    52081 ccacttcatc taagagaatc actgaatatg gtttttgacg aattttttct gtcaactgtc
    52141 caccttcatc ataccctacg tatcctggag gagacccgat taatcggctt gtactgtatt
    52201 tttccataaa ttctgacata tcaacacgga tcagggcatc ttcacttcca aacatgactt
    52261 ctgaaagtgc tttggccaat tctgtttttc cgacaccagt tggtccaagg aacatgaatg
    52321 aaccaattgg acgatcagga tctttcaatc cgcttcgtgc acggcggatc gcacgtgaaa
    52381 ctgctttgac agcctcatct tgaccaacga cacgttcatg cagaagtcct tccagttcta
    52441 acaagcgttc gctttctttt ttctccaact gctgtagagg gacaccagtc cactccgata
    52501 caactgtagc aacatcttct tctgttacac gatcagaata accttgctcc tgttttgctt
    52561 caattgcaga aactttttgc agctttttac gtagtgcttt ttcttgtctg cgtaattgag
    52621 cagcattttc aaagtcttgt ttttggatcg ctgcttcctt ttcttgtact agttcatcga
    52681 tttctagttt gatcacagtt gattttgtta agtgatcggt ttgatccaaa cggactttcg
    52741 ctgctgattc atcgatcaaa tcaattgctt tatcgggtag ttgacgagaa gtgatataac
    52801 gaacagaaag attgactgct gctcgtacag cttcatctgt gatctccact ccatggtgct
    52861 cttcatatct tgtacgtaat ccttgaagaa tcacttctgc ttcttcagga gttggctcat
    52921 ctacttgaat gcgagcaaaa cgtcgctcta atgcagaatc tttttcgata tatttttgat
    52981 attcatctag ggtcgttgct ccgatcgttt gtaattctcc tcgagctaac gccggtttca
    53041 agatattcga agcatcaatt gctccttctg ctccacctgc accaattaag gtatgcaatt
    53101 catcaatgaa taagattact tgaccatcat tatagatttc gtcgatgacc tttttcattc
    53161 gatcttcaaa ttcaccgcga tatttcgtac cagcaaccaa agatcccata tcaagcatca
    53221 tcaggcgctt ttcttgcatg tcttctggca cttcacctaa aatgattttt tgagctaacc
    53281 cttcagcaat ggctgttttg cctacaccag gttcacccac cagtacaggg ttattctttg
    53341 tccgtcggct aagaatctgg ataagccgtt tcacttctct cgaacgacca acaactgggt
    53401 ccagcttgtt ttcttttgct aatttcgtca aatcacgtgc taatgaatct agcgtaggtg
    53461 ttccttgcgg tgtttgccgt ttatcttgta catttacatt tctacgacga ttcattcctt
    53521 ttccagcaga atcaccagct agccccattt tttgtttcag aacttgacgg gcttttgaaa
    53581 ggctgatacc taagttgatc atgattcgag tagccatgat actttcatct tttaataagc
    53641 tcatcaaaat atgttctgtt ccaacttttt gagaaccagt tttcttcact tcgctgctag
    53701 ctaaagcaaa aacttgtttc attcttggtg aatatggaag aaagactcct ttagggtaag
    53761 aacggatacc accataacca atcagatgtt caatttcttc ataaatttcc gtttcattca
    53821 aatccatttc acgtaatact tttccagcaa ttccattttg ctcgaccact aaggctagta
    53881 aaacatgttc tgaactaact gattgatgtt tcaaccgttt tgcttcttct tgtgcaattt
    53941 ctaatactgc ttgggctctt tcagtaaaaa gttcttccat ctaatctcac ttcccatctt
    54001 cataacttaa acgttccaga aatgctcgga gaatccgagc acgtgtatga tcttcatatt
    54061 cattgaaatt caatgtgttt tttgcaattg cactcaacat caagtttcct tctttttttg
    54121 taattatttg gtcttcataa agtttttgaa taatagcaaa agcatttttc tcactgattg
    54181 tatcatcaaa taactgattt atctgctcaa gtaattgttt tttatctgaa atccttactt
    54241 ttgcaattcg tatataacca ccaccaccac gtttgctttc tactagataa ccacgttgga
    54301 tggtaaaacg ggtattaatc acatagttga tttgtgaagg aacacagtta aagaggtctg
    54361 ccatttcaat ccttcgaatc tcgatcattt cactattttc cagaattttc tttatatatg
    54421 attcaattaa atccgaggta ttctgatgtg ccatcttatt cattcccttt gactaatatt
    54481 gaccttaatt ataacgaatt tgataataga aagaaagaag aacgcttttt gttaaataaa
    54541 tcctttagaa tcaacaaaaa aaaatacgga atatactatt ccgtatc
//
LOCUS       DAYYAO010000008        50752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000008 DAYYAO010000000
VERSION     DAYYAO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.008"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..499
                     /locus_tag="ACHARI_000398"
     CDS             128..499
                     /locus_tag="ACHARI_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8441478.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(773..1465)
                     /locus_tag="ACHARI_000399"
     CDS             complement(773..1465)
                     /locus_tag="ACHARI_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8441479.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1468..2253)
                     /locus_tag="ACHARI_000400"
     CDS             complement(1468..2253)
                     /locus_tag="ACHARI_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8441480.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2490..3890
                     /gene="lacG"
                     /locus_tag="ACHARI_000401"
     CDS             2490..3890
                     /gene="lacG"
                     /locus_tag="ACHARI_000401"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8441481.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3915..4232
                     /locus_tag="ACHARI_000402"
     CDS             3915..4232
                     /locus_tag="ACHARI_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8441482.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4245..5918
                     /locus_tag="ACHARI_000403"
     CDS             4245..5918
                     /locus_tag="ACHARI_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8441483.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5932..7563
                     /locus_tag="ACHARI_000404"
     CDS             5932..7563
                     /locus_tag="ACHARI_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8441484.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8054..9619)
                     /gene="guaA"
                     /locus_tag="ACHARI_000405"
     CDS             complement(8054..9619)
                     /gene="guaA"
                     /locus_tag="ACHARI_000405"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8441485.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9678..10601)
                     /gene="coaA"
                     /locus_tag="ACHARI_000406"
     CDS             complement(9678..10601)
                     /gene="coaA"
                     /locus_tag="ACHARI_000406"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8441486.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10797..11402
                     /locus_tag="ACHARI_000407"
     CDS             10797..11402
                     /locus_tag="ACHARI_000407"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8441487.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11710..12726
                     /locus_tag="ACHARI_000408"
     CDS             11710..12726
                     /locus_tag="ACHARI_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8441488.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12867..14168
                     /locus_tag="ACHARI_000409"
     CDS             12867..14168
                     /locus_tag="ACHARI_000409"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8441489.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14214..14876
                     /gene="deoC"
                     /locus_tag="ACHARI_000410"
     CDS             14214..14876
                     /gene="deoC"
                     /locus_tag="ACHARI_000410"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8441490.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14897..15295
                     /locus_tag="ACHARI_000411"
     CDS             14897..15295
                     /locus_tag="ACHARI_000411"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8441491.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15361..16443
                     /locus_tag="ACHARI_000412"
     CDS             15361..16443
                     /locus_tag="ACHARI_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8441492.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16655..18217
                     /locus_tag="ACHARI_000413"
     CDS             16655..18217
                     /locus_tag="ACHARI_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441493.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18210..19343
                     /locus_tag="ACHARI_000414"
     CDS             18210..19343
                     /locus_tag="ACHARI_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441494.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19343..20299
                     /locus_tag="ACHARI_000415"
     CDS             19343..20299
                     /locus_tag="ACHARI_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441495.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20398..21564
                     /gene="deoB"
                     /locus_tag="ACHARI_000416"
     CDS             20398..21564
                     /gene="deoB"
                     /locus_tag="ACHARI_000416"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8441496.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21588..22406
                     /locus_tag="ACHARI_000417"
     CDS             21588..22406
                     /locus_tag="ACHARI_000417"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8441497.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22423..23130
                     /gene="deoD"
                     /locus_tag="ACHARI_000418"
     CDS             22423..23130
                     /gene="deoD"
                     /locus_tag="ACHARI_000418"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8441498.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23428..24114)
                     /gene="gpmA"
                     /locus_tag="ACHARI_000419"
     CDS             complement(23428..24114)
                     /gene="gpmA"
                     /locus_tag="ACHARI_000419"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8441499.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24286..24867)
                     /locus_tag="ACHARI_000420"
     CDS             complement(24286..24867)
                     /locus_tag="ACHARI_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8441500.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25000..25683)
                     /gene="rpiA"
                     /locus_tag="ACHARI_000421"
     CDS             complement(25000..25683)
                     /gene="rpiA"
                     /locus_tag="ACHARI_000421"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8441501.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26002..26415
                     /gene="rpsL"
                     /locus_tag="ACHARI_000422"
     CDS             26002..26415
                     /gene="rpsL"
                     /locus_tag="ACHARI_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8441502.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26499..26969
                     /gene="rpsG"
                     /locus_tag="ACHARI_000423"
     CDS             26499..26969
                     /gene="rpsG"
                     /locus_tag="ACHARI_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8441503.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27063..29150
                     /gene="fusA"
                     /locus_tag="ACHARI_000424"
     CDS             27063..29150
                     /gene="fusA"
                     /locus_tag="ACHARI_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8441504.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29322..30509
                     /gene="tuf"
                     /locus_tag="ACHARI_000425"
     CDS             29322..30509
                     /gene="tuf"
                     /locus_tag="ACHARI_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8441505.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30916..31224
                     /gene="rpsJ"
                     /locus_tag="ACHARI_000426"
     CDS             30916..31224
                     /gene="rpsJ"
                     /locus_tag="ACHARI_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8441506.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31255..31884
                     /gene="rplC"
                     /locus_tag="ACHARI_000427"
     CDS             31255..31884
                     /gene="rplC"
                     /locus_tag="ACHARI_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8441507.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31912..32535
                     /gene="rplD"
                     /locus_tag="ACHARI_000428"
     CDS             31912..32535
                     /gene="rplD"
                     /locus_tag="ACHARI_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8441508.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32535..32825
                     /gene="rplW"
                     /locus_tag="ACHARI_000429"
     CDS             32535..32825
                     /gene="rplW"
                     /locus_tag="ACHARI_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8441509.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32864..33697
                     /gene="rplB"
                     /locus_tag="ACHARI_000430"
     CDS             32864..33697
                     /gene="rplB"
                     /locus_tag="ACHARI_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8441510.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33740..34018
                     /gene="rpsS"
                     /locus_tag="ACHARI_000431"
     CDS             33740..34018
                     /gene="rpsS"
                     /locus_tag="ACHARI_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8441511.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34041..34388
                     /gene="rplV"
                     /locus_tag="ACHARI_000432"
     CDS             34041..34388
                     /gene="rplV"
                     /locus_tag="ACHARI_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8441512.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34402..35058
                     /gene="rpsC"
                     /locus_tag="ACHARI_000433"
     CDS             34402..35058
                     /gene="rpsC"
                     /locus_tag="ACHARI_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8441513.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35061..35495
                     /gene="rplP"
                     /locus_tag="ACHARI_000434"
     CDS             35061..35495
                     /gene="rplP"
                     /locus_tag="ACHARI_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8441514.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35485..35673
                     /gene="rpmC"
                     /locus_tag="ACHARI_000435"
     CDS             35485..35673
                     /gene="rpmC"
                     /locus_tag="ACHARI_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8441515.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35698..35964
                     /gene="rpsQ"
                     /locus_tag="ACHARI_000436"
     CDS             35698..35964
                     /gene="rpsQ"
                     /locus_tag="ACHARI_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8441516.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36022..36390
                     /gene="rplN"
                     /locus_tag="ACHARI_000437"
     CDS             36022..36390
                     /gene="rplN"
                     /locus_tag="ACHARI_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8441517.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36427..36735
                     /gene="rplX"
                     /locus_tag="ACHARI_000438"
     CDS             36427..36735
                     /gene="rplX"
                     /locus_tag="ACHARI_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8441518.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36763..37302
                     /gene="rplE"
                     /locus_tag="ACHARI_000439"
     CDS             36763..37302
                     /gene="rplE"
                     /locus_tag="ACHARI_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8441519.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37321..37506
                     /locus_tag="ACHARI_000440"
     CDS             37321..37506
                     /locus_tag="ACHARI_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8441520.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37542..37940
                     /gene="rpsH"
                     /locus_tag="ACHARI_000441"
     CDS             37542..37940
                     /gene="rpsH"
                     /locus_tag="ACHARI_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8441521.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37972..38508
                     /gene="rplF"
                     /locus_tag="ACHARI_000442"
     CDS             37972..38508
                     /gene="rplF"
                     /locus_tag="ACHARI_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8441522.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38669..39025
                     /gene="rplR"
                     /locus_tag="ACHARI_000443"
     CDS             38669..39025
                     /gene="rplR"
                     /locus_tag="ACHARI_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8441523.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39046..39546
                     /gene="rpsE"
                     /locus_tag="ACHARI_000444"
     CDS             39046..39546
                     /gene="rpsE"
                     /locus_tag="ACHARI_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8441524.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39561..39740
                     /gene="rpmD"
                     /locus_tag="ACHARI_000445"
     CDS             39561..39740
                     /gene="rpmD"
                     /locus_tag="ACHARI_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8441525.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39784..40224
                     /gene="rplO"
                     /locus_tag="ACHARI_000446"
     CDS             39784..40224
                     /gene="rplO"
                     /locus_tag="ACHARI_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8441526.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40224..41519
                     /gene="secY"
                     /locus_tag="ACHARI_000447"
     CDS             40224..41519
                     /gene="secY"
                     /locus_tag="ACHARI_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8441527.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41581..42228
                     /locus_tag="ACHARI_000448"
     CDS             41581..42228
                     /locus_tag="ACHARI_000448"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8441528.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42419..42637
                     /gene="infA"
                     /locus_tag="ACHARI_000449"
     CDS             42419..42637
                     /gene="infA"
                     /locus_tag="ACHARI_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8441529.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42670..42786
                     /gene="rpmJ"
                     /locus_tag="ACHARI_000450"
     CDS             42670..42786
                     /gene="rpmJ"
                     /locus_tag="ACHARI_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8441530.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42804..43169
                     /gene="rpsM"
                     /locus_tag="ACHARI_000451"
     CDS             42804..43169
                     /gene="rpsM"
                     /locus_tag="ACHARI_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8441531.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43197..43586
                     /gene="rpsK"
                     /locus_tag="ACHARI_000452"
     CDS             43197..43586
                     /gene="rpsK"
                     /locus_tag="ACHARI_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8441532.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43666..44604
                     /locus_tag="ACHARI_000453"
     CDS             43666..44604
                     /locus_tag="ACHARI_000453"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8441533.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44632..45012
                     /gene="rplQ"
                     /locus_tag="ACHARI_000454"
     CDS             44632..45012
                     /gene="rplQ"
                     /locus_tag="ACHARI_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8441534.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45284..46123
                     /locus_tag="ACHARI_000455"
     CDS             45284..46123
                     /locus_tag="ACHARI_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8441535.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46099..46968
                     /locus_tag="ACHARI_000456"
     CDS             46099..46968
                     /locus_tag="ACHARI_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8441536.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46958..47755
                     /locus_tag="ACHARI_000457"
     CDS             46958..47755
                     /locus_tag="ACHARI_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8441537.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47958..48704
                     /gene="truA"
                     /locus_tag="ACHARI_000458"
     CDS             47958..48704
                     /gene="truA"
                     /locus_tag="ACHARI_000458"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8441538.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49368..49562
                     /locus_tag="ACHARI_000459"
                     /pseudo
     CDS             49368..49562
                     /locus_tag="ACHARI_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49750..50526)
                     /locus_tag="ACHARI_000460"
     CDS             complement(49750..50526)
                     /locus_tag="ACHARI_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8441539.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50649..>50752
                     /locus_tag="ACHARI_000461"
     CDS             50649..>50752
                     /locus_tag="ACHARI_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFM8441540.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAV"
BASE COUNT        16085 a         9250 c        11024 g        14393 t
ORIGIN      
        1 aacgggttgg tttttaatag atttaataag tactgataat gaaatttctc ctggttggga
       61 aaacgaagtt ggttaccctg tagaagaatt ttttcaagat tactttgcgg agtatgccga
      121 taggataatg agagattacg taacagataa aactcaagaa gtcatcaatc aatcccagtc
      181 attaaatgaa ttatctgata aggcgaaaga agaagcagtt gccattattt cttcagggat
      241 tagtgactat attgatatga gtggtgaaat caattatggc gataaagaca aaattgtacg
      301 catgataaaa gcttttatca aaacttttga tgtgaaggca gatcttaagt ttaatgatac
      361 ttacctagta ggaaaattaa ttaatatcct tgggacagag aaagatacgg aaacgtttat
      421 ttcctctttg tctatgttgt ttacagataa gcatttttca actcagcttg gtggagagga
      481 actagtggaa atcttttaac cattacgccc agcattaata aaagtatatc aaaaaagtac
      541 tcctgatgat ttttaagatt ggtttgagaa ataatgtgaa aacactattt ttaatgttaa
      601 ttatagatag agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa agatacactg
      661 ctatatttca ctaaggatat agcagtgtat ctttcatgtc gtttcactgg cagtttttta
      721 taaaaggagg tgtgcatttc agttgctttt ctttggtagt tagttgaatc gtttaagaat
      781 taacggatgt ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg atggttccat
      841 ctttatttag ctgtgcctgt tcaaatactg ttttgttttt accgaatgga tcaattgcaa
      901 taattgttcc gatccccgca cgtttggcag caagtaatcc tgagtaagca tcttcgataa
      961 caagacaatc agccggagct aagctcagtt tgtctgccgc tttaagaaaa atatccggtt
     1021 gcggtttccc agggaaagag ccgtcgtcat agacaatctt gtcccagtca aaccagcggg
     1081 ctaaatcgaa aatatcaaaa taaaaagcaa cattctcttt gacagtagcc gtggcaatcg
     1141 ttaatggaat ctgttggtca gtcagctggt caagagtcgc ttcaagccct tttgtcaact
     1201 gaagctcgtt ttcattttgc aagcaaagca tacgataata gctttctttt tcttctgaca
     1261 atacggcgat ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat
     1321 tcgtccgacc atgaatattt tttaaaatat cctcgtctga taaaccatct tttgtatgat
     1381 catgtatcat atgcagccaa gcagcttcat gcagatgaga gtccaaaaac attgttccgt
     1441 tgaaatcaaa tataatccct ttcatagtta cgctcctata gtggtatttt cggttgaatc
     1501 agcaatttgc agatcgacat attgtgcata gtgctcttga acctcccgcg agatctggcg
     1561 atccgtaatg agcagatcga aatcatgcaa attataaaac acataaaaat catctcgatt
     1621 gaatttttta ttatcaacga gtagatattt cttacgggaa ttatttagag cgatctgctg
     1681 tgcttctcct tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact
     1741 gataaatgct tttgtaaact ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa
     1801 tgcgccggta tttttccgga aatcaccgcc aatcagtaga atttctggtc catctgcttg
     1861 agcaagaata tcaaaaactg gataattatt tgtaatgatc cgaattttct tgttcagtaa
     1921 atgcgtcgcc aatagttcga ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg
     1981 aatcaaggag gctgctgtgg cagcaatttc ttttttttct tgaatctgca gagtggcttt
     2041 ttcagtgtga gaaagttctt gatctaaagt gtaggaaaga ctttgagccc ctccatggac
     2101 acgcagcagc ttgcccgatt tttctaattc atctaaatct ctgcgaacgg tcatatcaga
     2161 aacatctaat tgattgatga tttcattaac ggtaaggatt ccttttttat tgaccatttc
     2221 tacgatagta agcaatcttt cttttttcaa cattgtcttc acctctaatt taaggataat
     2281 ctatcatata aacggacaac agtcaatcta attcgaacaa aagtaaacat ttagggtggt
     2341 ttttatgttt gttacaatag attttattgc tgttttctgg tttttatgat tatttgtgtt
     2401 tgtttttgtt gacaaatgtt ttggatgcgt ttacaatgca atcagttaga aagagcgaaa
     2461 actgattata gatttgagga gagatgtaca tgcttaaatt accagaagat tttatttttg
     2521 gcggagccac agcagcctat caggttgaag gagccactaa ggaaggtggc aaaggcgctg
     2581 ttgcttggga tgacttttta gaggaacaag ggcgattcag tcccgatccg gcaagtgatt
     2641 tttatcatca atatgcaaag gacattgaat tgtgtgaacg ttttggtgta aatggtctaa
     2701 gattatccat tgcttggagc cgcatttttc ctgatggagc tgggaaacca aatccagaag
     2761 gaatcgcttt ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg
     2821 tgacgttgca tcattttgat acccctaaac gtttgtttga ccatggagat tttttgaatc
     2881 gagaaacaat agaagcattt gtttcctatg ccattttttg ttttcatgaa ttcaaagaag
     2941 tcaaggtgtg gagtaccttt aatgaaatct atccagttgc aaccaatcaa tatttgttag
     3001 gtgttttccc accaggaatc aaatatgatt ttacaaagat cgtcgcctgt cttcataata
     3061 tgatggttgc tcatgcccgt gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa
     3121 ttggtgtagt tcactcatta gagacgaaat atgcggcaac agacgcgccg gaagataagc
     3181 atgctgcctt tttagacgat gcattgtcga tccgtttctt attagatgct acctatttag
     3241 gctattattc aactgaaacc ttgactgctc ttgatgagat atgtgaagca aatcaggcca
     3301 gctatcattt tcctgaagaa gatttcgttg aattgaaaaa agcatccacg cgtaatgact
     3361 acttagggat caatcactac caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa
     3421 tccatcataa cggtacgggt gaaaaaggga cctctgttta taaagtgaag ggaatcggag
     3481 aacgtattta taaagaagga attcctcgaa ctgattggga ctggctgatt tatccagaag
     3541 gtctatatga cttgttgctg cgaattaaaa gcgattaccc acattacaac aaaatctata
     3601 tcactgagaa cgggatgggg tataaagatc agtttgaaga tggcattatt atggatcagc
     3661 cgagaatcga ctatttgaga gtctatttgg aatccttaag caaggcgata acagccgggg
     3721 taaatgtgaa ggggtatttt ttgtggtcgc ttatggatct tttctcatgg acaaatgggt
     3781 ataacaaaag gtatggcttg ttttatgttg attttgaaac ccagaagcgt tacccaaaag
     3841 aatcagctta ctggtacaag ttggtcagtg aaaccaaaac gattatttaa taagtgataa
     3901 tggaggaaga aaagatgaat aaagaggaat tacaaatgct tggttttgag atcgttgcat
     3961 attcaggcga cgctcgcagt acattattga aattattgaa agaagtaaga caaggaaact
     4021 ttgaacatgt tgattcggcg ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt
     4081 cgcaaacaaa aattttagcg gaagaagcat ctgggaaaga gatggagatg ggattcattt
     4141 ttatccatgg gcaggatcat ttaatgacca ctttattgct gagagatctg attcaagact
     4201 ttattgtact gtacagacag aatcaaggat aggagaagaa cgctatggat ggaatcatta
     4261 aacaaatcga aaaagggaaa ccattttttg aaaaaatctc acgtaatagt tatttaggag
     4321 ccgttcgtga cggattttta acagcgatgc cagctatttt attttcaagc attttcattt
     4381 tgattgctgc ggttcctgag atttttggtt accaatggcc tgaatctgtc tccacatggc
     4441 tatggaaagt ctataattat tcgatgggtg ttgtagggtt actcgtttca gcaaccactg
     4501 ctcgctgctt ggctgattcg atgaatcgtc gcttaccgaa aaacaaagta atcaacacca
     4561 cctctgtgat gctggcctct gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag
     4621 gtggattttc aacggaatat atggggacaa agggactact ggcttccttt gtcgctgcat
     4681 ttttaacagt taatttatat aagttttgtg tgctgaaaga tattaccatt aaaatgccta
     4741 aagaagttcc tggaacgatt tcgcaaacct tccgagatat ttttcctttc tctttttcag
     4801 tatttgcggc cgttttaatt gatttaggca ttcgacaaat cttccaaatc tcatttgcag
     4861 aagcaatcat tacggtgctg cagccgctat ttacagcagc tgacgggtac ttaggaatcg
     4921 ctattatctg gggagcgatg gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg
     4981 tggagccggc aattgcggca attatctatg ccaatgtgga aaccaatctg caattgttca
     5041 attccggtga acaagcttct aaagtactga cggtgggttt agggaatttt gtaggaacta
     5101 tgggaggaac tggggcaaca ttggttgttc cttttatctt cctactcttt gctaagtcta
     5161 agcagttgaa ggcagttggg aaagcttcct ttattccagt aagtttcgct gtcaacgaac
     5221 cattgctatt tgcggcaccc atgattttga acccttattt ttttattcca tttttgttaa
     5281 cacctgttgt aaacgtttgc ctgttcaagt tttttgtcga tgtattgggc atgaacagct
     5341 ttatgtacgt tttgccttgg gcaactccag caccgatcgg tttggttcta ggaacgggca
     5401 tgggtatctt agcttttgtt ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc
     5461 taccattttg caaagcatac gatgctgtct tagtagagaa agaagcacaa aaagcagagg
     5521 tgatcgcaac cgaggaaggt gcttctgccg atgtacgtga ggaagtacct gtggcagcga
     5581 gtcaagttat cagcgatgca aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag
     5641 ggacaagtgc gatgttagct aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga
     5701 tcagtgcaat ggcgggtgct tatggttctc attacgaaat gctgcaggat tttgatatgg
     5761 tggtcttagc accgcaagtc aattcttact atgatgatat caaagaagac accgatcgct
     5821 taggaatcaa gctggttgcg acaaaaggag cagaatatat cggattaacg agagatcctg
     5881 aaaaagctgt tcagtttatt ctgaaccaat ttcaataaag gaggagccat aatgaaaatg
     5941 ggaatttggt tgcgtaaagc tgttttattt gcagctgtgt taggggcttt gggggctgtt
     6001 tcaaaaactg ttgaagcatc tgccagcgaa tttattcgcg gagcagatat ttcgatcctg
     6061 tcagatatgg aaaaatctgg tgcaaaatat tatgaagacg atatttcaaa agatgcctta
     6121 gagattttaa aaaataatgg tgtcaactat gttcgactgc gtttgtggca ggatccttat
     6181 aatagtaatg gagaaagtta tggagcaggg acaaatgatc tggaaacgac gattgacctt
     6241 gcaaaacgag caaaaaatct gggacttaag gtgctgctgg attttcacta cagtgatttt
     6301 tgggttgatc ctgggaaaca gaatcttcct aaagcatggc aaggtctgac ctttgaagaa
     6361 atgaatacgg cattgtacga ctatacaaag aacgttctgt cggaaatgaa gcagctggac
     6421 gtttatcctg atatggttca gatcggcaat gaactgaata gcggaatgct ctggccttat
     6481 ggcaagtctt ggggagaagg cgggggagaa tttgatcgat tggctgcttt tttgaagtct
     6541 ggtatccaag ctgttcgaga cactcaacct aaaacaactc cagtaatgct ccacttagct
     6601 gatggtggcg atacaggagc atttacttgg tggtttgatg aaatcacgag tcgaggtgtg
     6661 tcatttgatt taattggagt gtcgtattat ccgtattggc acggttcaat gaatgaactt
     6721 caaaccaata tgaatgcgat cagccaacgg tatggtaaag aagtgatcgt agttgaaaca
     6781 gcttatgggc atacaacagc gaatgccgat actatgccaa atgcttttgg tgaagccgaa
     6841 gcagctgcgg ggggatatga accaacaccg acaggacaag cagagtattt gctggatctt
     6901 gccgatcgga ttcaggcagt accgaataat cgcggggcgg gcttcttcta ttgggaacct
     6961 ctttggtaca acggaaatgt gagttgggca acacaagcag gtatgaatta tctcagtgtt
     7021 caatccacta tgggaaacga atgggacaac caagcgatgt ttgattttgc tggaaacgcc
     7081 ttgccaagct tgcgtgcgtt taaacaagca ggggcgcaaa taaatctagt taaaaatgca
     7141 agtttcgaaa gcgatggacc aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa
     7201 ggaaccgcta agggtacgat caaaacggaa gcgggcactt tttttgggga ctacaaacta
     7261 actttttggc aggatcaagc atacgaggca tcagcttatc aaaatgttag tggcataagc
     7321 aaaggaactt acacattgag tgcctatgta atgtctgggg gagagtttga tactaatcag
     7381 ttatatatca aaaattatgg tggagcggaa ctaaatactg cggttgttaa aacaagtgaa
     7441 tggacaaaaa ttacaatcaa agacattcca attacaacag gtgtttgtga aattggggtt
     7501 tatagcaagg cttcaggaaa tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa
     7561 tagactgcgg atcgtttcga ttcaagatcg gaggttccct tcagccacat cgttagcgta
     7621 gaaggcagga atcgaacctg cacctcgcct ttgccaaaaa taaccagaaa cgatttccag
     7681 tcatttttac caaaacgagt aaagcaccag acttcatcta cactttgtat tatgctaaag
     7741 agagttcaac gatttcgtaa gaagcaatga aaaaattgca aaaagttgtg cagataacat
     7801 aaagtttgtt taagaaaggg taaccttaga attccgtcgt gaatatgatt tgagcactta
     7861 gatggataga aaaaagttct cggaaaacag ttcgtttttc gagaacttct ttttatgata
     7921 agaatgaaac aaattaactt atattatagt ctcttttggt agggaaccat cttattttta
     7981 atgaaaatca agcattttag ctattgaaga atgttgattt atagggattc cccctccata
     8041 ttatgttggc tctttattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     8101 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     8161 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     8221 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     8281 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     8341 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     8401 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     8461 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     8521 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     8581 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     8641 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     8701 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     8761 ttgcattgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     8821 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     8881 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     8941 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     9001 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     9061 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     9121 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     9181 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     9241 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     9301 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     9361 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     9421 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     9481 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     9541 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     9601 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     9661 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     9721 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     9781 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     9841 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     9901 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     9961 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
    10021 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
    10081 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
    10141 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
    10201 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
    10261 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
    10321 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
    10381 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
    10441 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
    10501 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
    10561 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
    10621 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
    10681 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
    10741 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
    10801 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
    10861 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
    10921 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
    10981 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
    11041 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
    11101 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
    11161 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
    11221 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
    11281 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
    11341 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
    11401 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
    11461 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
    11521 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
    11581 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
    11641 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
    11701 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
    11761 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
    11821 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
    11881 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
    11941 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
    12001 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
    12061 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
    12121 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
    12181 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
    12241 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
    12301 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
    12361 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
    12421 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
    12481 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
    12541 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
    12601 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
    12661 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
    12721 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
    12781 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
    12841 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
    12901 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
    12961 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
    13021 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
    13081 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
    13141 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
    13201 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
    13261 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
    13321 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
    13381 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
    13441 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
    13501 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
    13561 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
    13621 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
    13681 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
    13741 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    13801 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    13861 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    13921 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    13981 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    14041 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    14101 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    14161 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    14221 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    14281 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    14341 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    14401 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    14461 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    14521 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    14581 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    14641 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    14701 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    14761 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    14821 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    14881 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    14941 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    15001 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    15061 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    15121 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    15181 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    15241 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    15301 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    15361 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    15421 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    15481 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    15541 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    15601 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    15661 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    15721 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    15781 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    15841 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    15901 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    15961 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    16021 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    16081 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    16141 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    16201 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    16261 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    16321 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    16381 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    16441 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    16501 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    16561 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    16621 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    16681 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    16741 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    16801 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    16861 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    16921 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    16981 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    17041 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    17101 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    17161 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    17221 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    17281 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    17341 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    17401 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    17461 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    17521 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    17581 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    17641 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    17701 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    17761 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    17821 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    17881 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    17941 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    18001 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    18061 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    18121 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    18181 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    18241 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    18301 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    18361 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    18421 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    18481 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    18541 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    18601 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    18661 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    18721 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    18781 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    18841 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    18901 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    18961 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    19021 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    19081 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    19141 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    19201 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    19261 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    19321 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    19381 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    19441 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    19501 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    19561 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    19621 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    19681 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    19741 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    19801 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    19861 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    19921 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    19981 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    20041 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    20101 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    20161 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    20221 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    20281 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    20341 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    20401 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    20461 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    20521 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    20581 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    20641 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    20701 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    20761 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    20821 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    20881 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    20941 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    21001 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    21061 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    21121 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    21181 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    21241 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    21301 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    21361 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    21421 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    21481 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    21541 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    21601 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    21661 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    21721 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    21781 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    21841 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    21901 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    21961 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    22021 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    22081 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    22141 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    22201 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    22261 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    22321 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    22381 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    22441 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    22501 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    22561 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    22621 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    22681 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    22741 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    22801 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    22861 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    22921 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    22981 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    23041 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    23101 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    23161 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    23221 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    23281 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    23341 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    23401 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    23461 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    23521 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    23581 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    23641 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    23701 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    23761 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    23821 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    23881 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    23941 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    24001 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    24061 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    24121 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    24181 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    24241 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    24301 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    24361 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    24421 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    24481 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    24541 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    24601 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    24661 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    24721 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    24781 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    24841 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    24901 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    24961 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    25021 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    25081 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    25141 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    25201 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    25261 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    25321 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    25381 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    25441 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    25501 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    25561 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    25621 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    25681 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    25741 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    25801 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    25861 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    25921 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    25981 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    26041 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    26101 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    26161 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    26221 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    26281 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    26341 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    26401 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    26461 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    26521 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    26581 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    26641 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    26701 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    26761 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    26821 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    26881 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    26941 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    27001 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    27061 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    27121 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    27181 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    27241 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    27301 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    27361 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    27421 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    27481 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    27541 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    27601 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    27661 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    27721 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    27781 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    27841 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    27901 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    27961 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    28021 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    28081 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    28141 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    28201 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    28261 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    28321 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    28381 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    28441 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    28501 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    28561 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    28621 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    28681 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    28741 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    28801 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    28861 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    28921 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    28981 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    29041 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    29101 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    29161 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    29221 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    29281 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    29341 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    29401 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    29461 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    29521 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    29581 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    29641 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    29701 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    29761 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    29821 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    29881 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    29941 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    30001 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    30061 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    30121 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    30181 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    30241 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    30301 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    30361 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    30421 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    30481 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    30541 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    30601 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    30661 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    30721 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    30781 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    30841 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    30901 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    30961 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    31021 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    31081 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    31141 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    31201 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    31261 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    31321 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    31381 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    31441 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    31501 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    31561 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    31621 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    31681 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    31741 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    31801 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    31861 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    31921 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    31981 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    32041 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    32101 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    32161 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    32221 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    32281 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    32341 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    32401 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    32461 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    32521 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    32581 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    32641 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    32701 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    32761 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    32821 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    32881 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    32941 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    33001 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    33061 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    33121 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    33181 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    33241 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    33301 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    33361 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    33421 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    33481 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    33541 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    33601 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    33661 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    33721 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    33781 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    33841 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    33901 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    33961 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    34021 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    34081 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    34141 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    34201 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    34261 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    34321 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    34381 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    34441 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    34501 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    34561 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    34621 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    34681 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    34741 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    34801 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    34861 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    34921 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    34981 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    35041 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    35101 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    35161 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    35221 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    35281 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    35341 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    35401 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    35461 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    35521 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    35581 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    35641 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    35701 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    35761 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    35821 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    35881 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    35941 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    36001 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    36061 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    36121 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    36181 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    36241 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    36301 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    36361 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    36421 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    36481 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    36541 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    36601 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    36661 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    36721 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    36781 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    36841 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    36901 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    36961 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    37021 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    37081 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    37141 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    37201 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    37261 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    37321 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    37381 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    37441 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    37501 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    37561 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    37621 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    37681 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    37741 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    37801 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    37861 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    37921 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    37981 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    38041 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    38101 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    38161 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    38221 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    38281 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    38341 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    38401 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    38461 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    38521 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    38581 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    38641 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    38701 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    38761 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    38821 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    38881 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    38941 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    39001 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    39061 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    39121 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    39181 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    39241 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    39301 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    39361 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    39421 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    39481 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    39541 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    39601 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    39661 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    39721 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    39781 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    39841 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    39901 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    39961 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    40021 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    40081 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    40141 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    40201 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    40261 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    40321 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    40381 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    40441 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    40501 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    40561 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    40621 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    40681 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    40741 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    40801 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    40861 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    40921 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    40981 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    41041 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    41101 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    41161 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    41221 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    41281 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    41341 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    41401 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    41461 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    41521 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    41581 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    41641 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    41701 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    41761 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    41821 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    41881 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    41941 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    42001 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    42061 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    42121 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    42181 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    42241 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    42301 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    42361 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    42421 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    42481 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    42541 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    42601 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    42661 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    42721 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    42781 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    42841 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    42901 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    42961 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    43021 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    43081 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    43141 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    43201 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    43261 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    43321 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    43381 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    43441 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    43501 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    43561 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    43621 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    43681 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    43741 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    43801 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    43861 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    43921 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    43981 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    44041 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    44101 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    44161 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    44221 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    44281 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    44341 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    44401 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    44461 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    44521 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    44581 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    44641 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    44701 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    44761 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    44821 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    44881 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    44941 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    45001 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    45061 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    45121 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    45181 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    45241 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    45301 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    45361 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    45421 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    45481 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatggt
    45541 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    45601 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    45661 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    45721 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    45781 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    45841 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    45901 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    45961 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    46021 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    46081 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    46141 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    46201 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    46261 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    46321 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    46381 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    46441 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    46501 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    46561 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    46621 ggacttgatc cccagggtag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    46681 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    46741 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    46801 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    46861 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    46921 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    46981 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    47041 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    47101 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    47161 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    47221 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    47281 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    47341 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    47401 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    47461 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    47521 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    47581 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    47641 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    47701 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttgagag gataaacgtt
    47761 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcgagcagt
    47821 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    47881 tgaaaagaaa gaacgaaaaa attttataaa aaaatcatta ttccagataa ccttttagga
    47941 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcatacgac ggaacaaatt
    48001 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    48061 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    48121 gagtacatgc gattgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    48181 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    48241 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    48301 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    48361 ctcttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    48421 cctctttttg tgcatcagga agctcgatag aggataaagt acgtacgatc tacgaggcta
    48481 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    48541 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    48601 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    48661 cacatcctga aggactttat ttgtatgaag tgaaatatga ataagaggtt gtgacagaag
    48721 tggtcagctt caagaaataa gaaggaattt acgaaaatta cttctcaaaa ttttgcaaaa
    48781 tttcggctta ttttcgaagg agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa
    48841 gctgccctgc atcaagtaat aagaacaacc aaacaaaaat agctcgtcat atttttcgtt
    48901 tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    48961 aatcagcgtt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    49021 ccgtttgttg agctaatacc gaaggtcagc tgattgaaca ccgtttaaga aagaggtcgt
    49081 gagcctgtca tttaactctg agtcacaagt aacaatttca aaaaacagct tctcatcttt
    49141 gatgaatatc gtgacttgtg gacgaggcgt tgataagtga atcatctttt tgttacctat
    49201 tttttctgta accaaaaaac gatggaacgt tgttttgatt gcccttaatg aggacagtcg
    49261 gaacaggttt gtcattttta tatttaaatg gcacaaattt ttctaattat ttgtcattta
    49321 gcgctgaaaa tttttgctat ttatctaaaa aagtgataac tacttaagat gaagcagaat
    49381 atttacgcca tacggcatac aaccgcgaaa aatataaaaa aaagaaaacg attgaacgcg
    49441 tttttgcagt cgcaaaggaa aagcatggaa tgcgctacat caagtatcga gacttggaaa
    49501 acaatcaggt gtatacgctg attgtttttg ctgcaatgaa tctgaaaaag ttgtccactt
    49561 ggcagttgga acatatgtct tcatatcttt tattatacaa aaatagcaaa agcaccagtc
    49621 tacactctta gtaaagagga aactgatcct tttgtctaca ttcttaaagt acttaaacta
    49681 agtactttat tttactcttt aggattgata cagtagcatc tatcccttta ttaataatct
    49741 tttatttttt taaataaaga tgttgtctcc atcttcaggc actaaaactt tttcggaaat
    49801 tcctttttct ttattgaaag actttaattc ttctcttgac agtccccaat ggtttacagc
    49861 ttccatatga acggcaataa ttgtggaatc tggtattgct ttatgcaatt catataaatc
    49921 gtctttcccc ataataagag attctccaac taaaaaatga ttatctccag catttgctac
    49981 tatgatttct ggattatact gatcaatagt ttgtttaaca gcttcatacc aaactgtatc
    50041 tcctgtaaag tatagtgtct tctcagtcga atgtttgaaa acaataccgc aaacattacc
    50101 tacataattc aagatttctc ctctgccatg tttgcctttt gttttaacca attgtataga
    50161 ttcaaaaact gtgtcttctc gtagtacctc cacattttta aaaccatata gtcgaatttc
    50221 attcgcatca ttttcatctt gcacaaataa ttttatttct ttaggtagga cctctttcgc
    50281 tatatcgtcc caatgatcta gatgtaaatg tgttacaata acagcatcaa taccgttgat
    50341 aatatcttcg actggcattg gcaaatcaac tagaggattc atttgatctt ttcttggtga
    50401 tggaaaagga cctttcgaaa taaatggtgg aagtgttcct ttttccgcta acattggatc
    50461 aattaaaaat tttttacctc catagtctaa gatagctgtt gcattacgaa tgtgatgtat
    50521 attcataaac gttgtctcct ttttgttgat atgaaatatt tttagtataa ttcaattcca
    50581 tacaataaat aaaccgaaca aaaaaagtct tttactattt ttacttaatt tatgaaaggt
    50641 tgtttaccat gttagaccaa acagatataa atatcattga ggaactatca aaaaatagtc
    50701 gaatcacaat gaaagaactt ggagaaaaag tacatttgac tggacccgct gt
//
LOCUS       DAYYAO010000009        50735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000009 DAYYAO010000000
VERSION     DAYYAO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.009"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            558..3197
                     /locus_tag="ACHARI_000462"
     CDS             558..3197
                     /locus_tag="ACHARI_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8441541.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="ACHARI_000463"
     CDS             complement(3365..4063)
                     /locus_tag="ACHARI_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8441542.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="ACHARI_000464"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="ACHARI_000464"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8441543.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="ACHARI_000465"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="ACHARI_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8441544.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="ACHARI_000466"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="ACHARI_000466"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8441545.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="ACHARI_000467"
     CDS             9034..10140
                     /locus_tag="ACHARI_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8441546.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="ACHARI_000468"
     CDS             10157..11185
                     /locus_tag="ACHARI_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8441547.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="ACHARI_000469"
     CDS             11191..11670
                     /locus_tag="ACHARI_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8441548.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="ACHARI_000470"
     CDS             11686..12981
                     /locus_tag="ACHARI_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8441549.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="ACHARI_000471"
     CDS             12992..13816
                     /locus_tag="ACHARI_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8441550.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="ACHARI_000472"
     CDS             13981..14880
                     /locus_tag="ACHARI_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441551.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="ACHARI_000473"
     CDS             complement(15006..15875)
                     /locus_tag="ACHARI_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8441552.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="ACHARI_000474"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="ACHARI_000474"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8441553.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="ACHARI_000475"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="ACHARI_000475"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8441554.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="ACHARI_000476"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="ACHARI_000476"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8441555.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="ACHARI_000477"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="ACHARI_000477"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8441556.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="ACHARI_000478"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="ACHARI_000478"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8441557.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="ACHARI_000479"
     CDS             21273..22031
                     /locus_tag="ACHARI_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8441558.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="ACHARI_000480"
     CDS             22131..22775
                     /locus_tag="ACHARI_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8441559.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="ACHARI_000481"
     CDS             22782..23789
                     /locus_tag="ACHARI_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8441560.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="ACHARI_000482"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="ACHARI_000482"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8441561.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="ACHARI_000483"
     CDS             24667..25470
                     /locus_tag="ACHARI_000483"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8441562.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /gene="fni"
                     /locus_tag="ACHARI_000484"
     CDS             complement(25719..26783)
                     /gene="fni"
                     /locus_tag="ACHARI_000484"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8441563.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="ACHARI_000485"
     CDS             complement(26780..27865)
                     /locus_tag="ACHARI_000485"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8441564.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="ACHARI_000486"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="ACHARI_000486"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8441565.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="ACHARI_000487"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="ACHARI_000487"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8441566.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30108..30767
                     /locus_tag="ACHARI_000488"
     CDS             30108..30767
                     /locus_tag="ACHARI_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8441567.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            30852..31757
                     /locus_tag="ACHARI_000489"
     CDS             30852..31757
                     /locus_tag="ACHARI_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8441568.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GRGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="ACHARI_000490"
     CDS             complement(31759..32391)
                     /locus_tag="ACHARI_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8441569.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    32542..32667
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="ACHARI_000491"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="ACHARI_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8441570.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33507
                     /gene="rpmI"
                     /locus_tag="ACHARI_000492"
     CDS             33307..33507
                     /gene="rpmI"
                     /locus_tag="ACHARI_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8441571.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            33560..33919
                     /gene="rplT"
                     /locus_tag="ACHARI_000493"
     CDS             33560..33919
                     /gene="rplT"
                     /locus_tag="ACHARI_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8441572.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34171..35508
                     /locus_tag="ACHARI_000494"
     CDS             34171..35508
                     /locus_tag="ACHARI_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8441573.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35556..37685
                     /locus_tag="ACHARI_000495"
     CDS             35556..37685
                     /locus_tag="ACHARI_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8441574.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37660..38349
                     /locus_tag="ACHARI_000496"
     CDS             37660..38349
                     /locus_tag="ACHARI_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441575.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38757..39370)
                     /locus_tag="ACHARI_000497"
                     /pseudo
     CDS             complement(38757..39370)
                     /locus_tag="ACHARI_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39506..39700)
                     /locus_tag="ACHARI_000498"
     CDS             complement(39506..39700)
                     /locus_tag="ACHARI_000498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441576.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39750..40190)
                     /locus_tag="ACHARI_000499"
     CDS             complement(39750..40190)
                     /locus_tag="ACHARI_000499"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8441577.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40194..41039)
                     /locus_tag="ACHARI_000500"
     CDS             complement(40194..41039)
                     /locus_tag="ACHARI_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8441578.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41188..42606
                     /gene="pepV"
                     /locus_tag="ACHARI_000501"
     CDS             41188..42606
                     /gene="pepV"
                     /locus_tag="ACHARI_000501"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8441579.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42663..43610)
                     /locus_tag="ACHARI_000502"
     CDS             complement(42663..43610)
                     /locus_tag="ACHARI_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8441580.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(43833..44183)
                     /locus_tag="ACHARI_000503"
     CDS             complement(43833..44183)
                     /locus_tag="ACHARI_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8441581.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            44383..45462
                     /gene="pepA"
                     /locus_tag="ACHARI_000504"
     CDS             44383..45462
                     /gene="pepA"
                     /locus_tag="ACHARI_000504"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8441582.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            45606..45926
                     /locus_tag="ACHARI_000505"
     CDS             45606..45926
                     /locus_tag="ACHARI_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8441583.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            45948..46412
                     /locus_tag="ACHARI_000506"
     CDS             45948..46412
                     /locus_tag="ACHARI_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8441584.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            46617..47222
                     /gene="ytpR"
                     /locus_tag="ACHARI_000507"
     CDS             46617..47222
                     /gene="ytpR"
                     /locus_tag="ACHARI_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8441585.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(47261..48550)
                     /locus_tag="ACHARI_000508"
     CDS             complement(47261..48550)
                     /locus_tag="ACHARI_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8441586.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            48978..49457
                     /gene="rlmH"
                     /locus_tag="ACHARI_000509"
     CDS             48978..49457
                     /gene="rlmH"
                     /locus_tag="ACHARI_000509"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8441587.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            49542..50051
                     /locus_tag="ACHARI_000510"
     CDS             49542..50051
                     /locus_tag="ACHARI_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFM8441588.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            50151..>50735
                     /locus_tag="ACHARI_000511"
     CDS             50151..>50735
                     /locus_tag="ACHARI_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441589.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLER"
BASE COUNT        16129 a         9193 c        10898 g        14515 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gacggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    33481 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    33541 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    33601 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    33661 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    33721 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    33781 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    33841 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    33901 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    33961 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    34021 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    34081 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    34141 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    34201 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    34261 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    34321 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    34381 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    34441 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    34501 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    34561 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    34621 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    34681 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    34741 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    34801 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    34861 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    34921 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    34981 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    35041 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    35101 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    35161 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    35221 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    35281 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    35341 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    35401 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    35461 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    35521 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    35581 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    35641 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    35701 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    35761 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    35821 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    35881 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    35941 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    36001 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    36061 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    36121 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    36181 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    36241 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    36301 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    36361 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    36421 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    36481 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    36541 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    36601 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    36661 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    36721 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    36781 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    36841 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    36901 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    36961 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    37021 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    37081 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    37141 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    37201 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    37261 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    37321 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    37381 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    37441 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    37501 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    37561 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    37621 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    37681 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    37741 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    37801 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    37861 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    37921 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    37981 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    38041 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    38101 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    38161 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    38221 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    38281 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    38341 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    38401 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    38461 tttgaaattg ttccttgtga ctcggagatg aatggcaggc tcacgacctc ttggaaaacg
    38521 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    38581 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    38641 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    38701 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    38761 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    38821 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    38881 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    38941 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    39001 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    39061 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    39121 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    39181 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    39241 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    39301 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    39361 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    39421 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    39481 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    39541 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    39601 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    39661 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    39721 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    39781 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    39841 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    39901 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    39961 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    40021 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    40081 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    40141 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    40201 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    40261 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    40321 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    40381 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    40441 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    40501 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    40561 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    40621 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    40681 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    40741 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    40801 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    40861 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    40921 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    40981 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    41041 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    41101 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    41161 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    41221 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    41281 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    41341 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    41401 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    41461 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    41521 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    41581 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    41641 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    41701 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    41761 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    41821 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    41881 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    41941 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    42001 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    42061 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    42121 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    42181 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    42241 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    42301 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    42361 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    42421 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    42481 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    42541 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    42601 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    42661 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    42721 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    42781 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    42841 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    42901 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    42961 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    43021 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    43081 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    43141 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    43201 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    43261 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    43321 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    43381 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    43441 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    43501 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    43561 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    43621 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    43681 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    43741 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    43801 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    43861 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    43921 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    43981 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    44041 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    44101 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    44161 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    44221 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    44281 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    44341 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    44401 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    44461 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    44521 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    44581 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    44641 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    44701 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    44761 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    44821 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    44881 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    44941 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    45001 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    45061 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    45121 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    45181 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    45241 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    45301 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    45361 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    45421 ttggataaat caacagtgaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    45481 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    45541 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    45601 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    45661 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    45721 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    45781 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    45841 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    45901 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    45961 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    46021 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    46081 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    46141 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    46201 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    46261 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    46321 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    46381 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    46441 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    46501 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    46561 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    46621 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    46681 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    46741 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    46801 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    46861 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    46921 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    46981 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    47041 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    47101 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    47161 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    47221 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    47281 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    47341 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    47401 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    47461 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    47521 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    47581 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    47641 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    47701 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    47761 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    47821 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    47881 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    47941 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    48001 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    48061 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    48121 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    48181 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    48241 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    48301 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    48361 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    48421 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    48481 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    48541 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    48601 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    48661 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    48721 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    48781 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    48841 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    48901 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    48961 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    49021 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    49081 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    49141 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    49201 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    49261 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    49321 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    49381 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    49441 cgccttatca taagtgatgc ggtttttatc attgatccta aaaatcttag caagacaaaa
    49501 agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac aatgataggt
    49561 tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa gcttttgagc
    49621 taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa gtacaggata
    49681 agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac caagtttttg
    49741 acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat aagccgatag
    49801 tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat
    49861 taaaaatcgc cttgtctatg accactgatg atattttaga cgtttttgct gaggcagaaa
    49921 tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa cgaaatttta
    49981 agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt tataatcgga
    50041 gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat gagagctagt
    50101 ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt atgaatgatg
    50161 aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga tgtaggctca
    50221 attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta gcgaataaat
    50281 cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt agtagaagcg
    50341 aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg ttaaataaga
    50401 gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata attgattcat
    50461 taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat ggaatggtca
    50521 ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt ttttatcaac
    50581 tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa tatggcaatg
    50641 aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct tggataattc
    50701 cggttgagtc aggagatgct aagaaactgg agaga
//
LOCUS       DAYYAO010000010        49095 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000010 DAYYAO010000000
VERSION     DAYYAO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.010"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..844
                     /locus_tag="ACHARI_000512"
     CDS             32..844
                     /locus_tag="ACHARI_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8441590.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            841..1641
                     /locus_tag="ACHARI_000513"
     CDS             841..1641
                     /locus_tag="ACHARI_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8441591.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1802..3070
                     /gene="rseP"
                     /locus_tag="ACHARI_000514"
     CDS             1802..3070
                     /gene="rseP"
                     /locus_tag="ACHARI_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8441592.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3138..4847
                     /locus_tag="ACHARI_000515"
     CDS             3138..4847
                     /locus_tag="ACHARI_000515"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8441593.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5059..9417
                     /locus_tag="ACHARI_000516"
     CDS             5059..9417
                     /locus_tag="ACHARI_000516"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8441594.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9562..10035
                     /gene="rimP"
                     /locus_tag="ACHARI_000517"
     CDS             9562..10035
                     /gene="rimP"
                     /locus_tag="ACHARI_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8441595.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10058..11233
                     /gene="nusA"
                     /locus_tag="ACHARI_000518"
     CDS             10058..11233
                     /gene="nusA"
                     /locus_tag="ACHARI_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8441596.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11255..11548
                     /locus_tag="ACHARI_000519"
     CDS             11255..11548
                     /locus_tag="ACHARI_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8441597.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11545..11856
                     /locus_tag="ACHARI_000520"
     CDS             11545..11856
                     /locus_tag="ACHARI_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8441598.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11869..14175
                     /gene="infB"
                     /locus_tag="ACHARI_000521"
     CDS             11869..14175
                     /gene="infB"
                     /locus_tag="ACHARI_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8441599.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14202..14549
                     /gene="rbfA"
                     /locus_tag="ACHARI_000522"
     CDS             14202..14549
                     /gene="rbfA"
                     /locus_tag="ACHARI_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8441600.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14706..15162)
                     /locus_tag="ACHARI_000523"
                     /pseudo
     CDS             complement(14706..15162)
                     /locus_tag="ACHARI_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15327..17689
                     /locus_tag="ACHARI_000524"
                     /pseudo
     CDS             15327..17689
                     /locus_tag="ACHARI_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18057..18980
                     /gene="truB"
                     /locus_tag="ACHARI_000525"
     CDS             18057..18980
                     /gene="truB"
                     /locus_tag="ACHARI_000525"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8441601.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18984..19925
                     /gene="ribF"
                     /locus_tag="ACHARI_000526"
     CDS             18984..19925
                     /gene="ribF"
                     /locus_tag="ACHARI_000526"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8441602.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20277..21062
                     /locus_tag="ACHARI_000527"
     CDS             20277..21062
                     /locus_tag="ACHARI_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8441603.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21075..21926
                     /locus_tag="ACHARI_000528"
     CDS             21075..21926
                     /locus_tag="ACHARI_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8441604.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22050..22502)
                     /locus_tag="ACHARI_000529"
     CDS             complement(22050..22502)
                     /locus_tag="ACHARI_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441605.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22967..23335)
                     /locus_tag="ACHARI_000530"
     CDS             complement(22967..23335)
                     /locus_tag="ACHARI_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8441606.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23485..23967
                     /locus_tag="ACHARI_000531"
     CDS             23485..23967
                     /locus_tag="ACHARI_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8441607.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24004..25014
                     /locus_tag="ACHARI_000532"
     CDS             24004..25014
                     /locus_tag="ACHARI_000532"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8441608.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25842)
                     /locus_tag="ACHARI_000533"
     CDS             complement(25129..25842)
                     /locus_tag="ACHARI_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441609.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26015..26622)
                     /locus_tag="ACHARI_000534"
                     /pseudo
     CDS             complement(26015..26622)
                     /locus_tag="ACHARI_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            27106..27333
                     /locus_tag="ACHARI_000535"
     CDS             27106..27333
                     /locus_tag="ACHARI_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8441610.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27349..28515
                     /gene="hemW"
                     /locus_tag="ACHARI_000536"
     CDS             27349..28515
                     /gene="hemW"
                     /locus_tag="ACHARI_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8441611.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28749..29792
                     /gene="hrcA"
                     /locus_tag="ACHARI_000537"
     CDS             28749..29792
                     /gene="hrcA"
                     /locus_tag="ACHARI_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8441612.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29886..30449
                     /gene="grpE"
                     /locus_tag="ACHARI_000538"
     CDS             29886..30449
                     /gene="grpE"
                     /locus_tag="ACHARI_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8441613.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30500..32329
                     /gene="dnaK"
                     /locus_tag="ACHARI_000539"
     CDS             30500..32329
                     /gene="dnaK"
                     /locus_tag="ACHARI_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8441614.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32480..33646
                     /gene="dnaJ"
                     /locus_tag="ACHARI_000540"
     CDS             32480..33646
                     /gene="dnaJ"
                     /locus_tag="ACHARI_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8441615.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33728..34357)
                     /locus_tag="ACHARI_000541"
     CDS             complement(33728..34357)
                     /locus_tag="ACHARI_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441616.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            34369..34542
                     /locus_tag="ACHARI_000542"
     CDS             34369..34542
                     /locus_tag="ACHARI_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441617.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34587..35324)
                     /locus_tag="ACHARI_000543"
     CDS             complement(34587..35324)
                     /locus_tag="ACHARI_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8441618.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35326..36828)
                     /locus_tag="ACHARI_000544"
     CDS             complement(35326..36828)
                     /locus_tag="ACHARI_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8441619.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37087..39081
                     /gene="uvrB"
                     /locus_tag="ACHARI_000545"
     CDS             37087..39081
                     /gene="uvrB"
                     /locus_tag="ACHARI_000545"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8441620.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39091..41907
                     /gene="uvrA"
                     /locus_tag="ACHARI_000546"
     CDS             39091..41907
                     /gene="uvrA"
                     /locus_tag="ACHARI_000546"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8441621.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42170..43435
                     /locus_tag="ACHARI_000547"
     CDS             42170..43435
                     /locus_tag="ACHARI_000547"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8441622.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43439..44170
                     /locus_tag="ACHARI_000548"
     CDS             43439..44170
                     /locus_tag="ACHARI_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8441623.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44305..45189
                     /gene="rapZ"
                     /locus_tag="ACHARI_000549"
     CDS             44305..45189
                     /gene="rapZ"
                     /locus_tag="ACHARI_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8441624.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45186..46184
                     /gene="yvcK"
                     /locus_tag="ACHARI_000550"
     CDS             45186..46184
                     /gene="yvcK"
                     /locus_tag="ACHARI_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8441625.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46218..47153
                     /gene="whiA"
                     /locus_tag="ACHARI_000551"
     CDS             46218..47153
                     /gene="whiA"
                     /locus_tag="ACHARI_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8441626.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47696..48346
                     /locus_tag="ACHARI_000552"
     CDS             47696..48346
                     /locus_tag="ACHARI_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441627.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48533..>49095
                     /locus_tag="ACHARI_000553"
     CDS             48533..>49095
                     /locus_tag="ACHARI_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM8441628.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16419 a         8416 c        10567 g        13693 t
ORIGIN      
        1 tgggaatctt ttaagtatag gaggaaaaag aatgttacgt ttttttccgc aaaaaaacaa
       61 atatattcaa gaaaaaagcg aatttcaatt taataccgat ggaccaattc ctcagcacat
      121 tgccatcatc atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc cacgagttgc
      181 tggccataaa gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat caaggcttgg
      241 agtgaaagtg ttgacgcttt atgccttttc gactgaaaac tggaagcgac caaaagatga
      301 agtaagtttt ctcatgcaac tacctgtcga cttctttgat acgttcgtac cagaattgat
      361 taaagaaaat gtcaaagttc atgtcatggg ttatgaaaac gtattgccag aacatacgca
      421 agatgctgta agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg tattgaattt
      481 tgctttgaat tatggaagca gagcagagat cgtgactgct gtccaagaaa ttgccgaaga
      541 agtagcaaaa ggagaaatac acgcagaaga aatcgatgat gaattgattg ctaagcattt
      601 aatgacaggt ttcttgccta aagagttaca agatcctgag ctgatgatcc gtacaagtgg
      661 agaagagcga attagtaatt ttctgttatg gcagatcgca tatagtgagc tgtattttac
      721 aaaagcgctt tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg cctcttatca
      781 aaatcgagac cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag gggatcagtc
      841 atgaaacaac gtgtggtcac agcagccgtg gcgctagtct tgtttattcc gattatatgg
      901 atcggaggca tagcggttga gcttacagca gcaatcttag cagtaatagg agtttacgag
      961 ctgtttcgga tgaaaggatt gacactctta agttttgaag gagttttatc cgccgttggt
     1021 gcagtgttac ttgttttgcc aaaagaaaga tggtttttct ttcttccaga gaatgtaagt
     1081 acatttatgt tattttatct tatagttatg attcttttag gaacatcagt tatttctaaa
     1141 aatacgtata cgattgatga agcaggtttt cctgtgcttg tcagcttgta tgtgggaata
     1201 ggtttccaga attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat atttggtcta
     1261 tttattgttt gggcaactga tatcggagca tatatggttg gtcgacgtta tggtcagcat
     1321 aagttaagtc ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg tggtattggt
     1381 agtgctgttg tagtcgcatt tctttattta ttgctatacc cagcaaaaga actatttaat
     1441 catggtatgt ttgtcatgct tctgatgacg attatttttt cagcagtcgg acaatttggt
     1501 gatttggtcg aatctgcaat caaacgtcat tatgatgtga aagactcggg aaatatctta
     1561 ccaggccatg gtggaatctt agaccgtttc gacagtttgc tgtttgtatt tcctatcatg
     1621 catttatttg gcattattta gtaagtatac ctgcgtttct ttgtgaattt cataaaagac
     1681 gcaggttttt atttttcatt atattgatac tattggtgtc tttggtatac tatggtaaaa
     1741 ttatagtgtt ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg agaaaaaagc
     1801 tatgaaaacg attctgacat ttatcatcgt ttttggtata ttagtgattg ttcatgagtt
     1861 tggtcatttc ttctttgcga aacgatcagg aatcctcgtt cgcgaatttg ctatcggtat
     1921 gggtccgaaa atttatgggc atcaagcaaa agacgggaca acatatacgc ttcgcctatt
     1981 acctattggc ggttatgttc gaatggcagg caatggggat gatgaaacag aaatggcacc
     2041 aggaatgcct ctttctcttt tactaaattc tgacggcatc gtagagaaga taaatttaag
     2101 taagaaaatc caattgacaa atgctatccc gatggaattg agccgttatg atttagaaga
     2161 tgagctgact atcactggtt atgtgaatgg agacgaaaca gaagtagtaa cctaccctgt
     2221 tgatcacgat gcgacgatta ttgaaaacga cggaacagaa atcagaattg cgccaaaaga
     2281 cgtacagttc caatcggcta aattgtggca gcgtatgctg accaactttg ctggacccat
     2341 gaataacttt attttggcaa tcctgttatt catcattctg gcatttatgc aaggtggtgt
     2401 tcaagtgacg aacactaatc gcgtaggaga gatcatgcct aatggagctg cggcagaagc
     2461 aggcttaaaa gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc attcatggaa
     2521 tgatctgaca acagtcatta cgaaaaatcc ggataaaaca ttagacttta agatcgaacg
     2581 agaaggtcag gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa atggcgaaaa
     2641 agttggccaa cttggtataa aagccccaat gaatacaggt tttatggata agatcattgg
     2701 agggacgcgt caagctttca gcggttcatt ggaaattttc aaagccttag gatcactatt
     2761 tacaggtttc agtttagata aattaggcgg acctgtcatg atgtaccaat tgtcttcaga
     2821 agcagcaaat caaggtgtaa caactgttat tagtttgatg gcgcttcttt caatgaatct
     2881 cggaatcgtc aatctgcttc cgattcctgc cttagatggc gggaaattag tcttgaatat
     2941 ttttgaaggg attcgcggga aaccattaag tcaagaaaaa gaaggaatat tgacattagc
     3001 tggctttgga tttttaatgt tgttgatggt gctagttaca tggaacgata ttcaacgatt
     3061 ctttttctag aaaaagttga caaatagggt ataataacaa acgataacta ttttgaggaa
     3121 ataaaggagt atatagaatg agacaatcaa aaatgttgat tccaacatta cgcgaagttc
     3181 caaatgatgc agaagtatta agccatcaaa tcttgctaag agctggatat atccgccaag
     3241 tatcagcggg tatttattca tatctgccat tagcaaatcg tgtattggaa aaactgaaac
     3301 gcattatgcg ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg cctgccatct
     3361 tgccagcaga actttgggaa gaatccgggc gttacgaaac atatggacca aacttgtatc
     3421 gtttcaaaga ccgcaatgat cgaaaaatga tccttggacc aactcacgaa gaaactttta
     3481 ctgatttgat gcgtaatgaa atcaattcat ataagaaatt acctttaaat ctttatcaaa
     3541 tccaacctaa atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc ggaagagagt
     3601 ttatcatgaa agatgcgtat tctttccatg caacggaaga aagtttggac gagacatata
     3661 aagattacga aaaagcctac accgaagtat tcaagcgttg tggcttagaa tttcgctcga
     3721 ttatcggaga cggtggcgca atgggcggga aagactcaaa agaattcatg gcgatctcag
     3781 aaatcgggga ggatacgatc tgttattcga ctgaaagtga ttatgctgca aacttggaga
     3841 tggctacaag ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa ttggagaaag
     3901 tagctacacc taacgtaaaa tcaattgacg aagtagcaga tttcttttct gttgaacctc
     3961 aaaaaatcat caagtctgta ttgttcattg cggatgaaca acctgtcctt gttttggtac
     4021 gcggagacca tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct gactttttag
     4081 aagaagcaac agaagaagaa gcacaaaaat atctaggtgc tgagtttggt tctgtcggac
     4141 cagtcggtgt ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa gatttagcga
     4201 atgccattac tggagcgaac gaaacaggat tccactataa aaatgtgaat ccagaacgcg
     4261 atttcactgt actttcttat gaagatcttc gttttgtaca agaaggagat ccttcaccag
     4321 acaacaatgg tgttcttgcc tttactcgag gaatcgaaat cggacatatc ttcaaaatcg
     4381 gcactcgtta cagtgaatct atgggagcaa ctgtactaga cgaaaatgga cgagaaaaat
     4441 ttgtgattat gggctgttat ggtatcggtg taagtcgttt attatcagca atcgtagaac
     4501 aacattcaga tgaacaagga atcaactggc caaaaggaat tgcgccattt gatttgcata
     4561 tcgtccaaat gaatctaaaa gacgaatacc aaacaaacct gacgaatgaa ctagaagaag
     4621 cgatgacaaa agctggctat gaagtactgg ttgatgacag aaatgaacgc gcaggagtga
     4681 aattcgcgga ttctgatttg attggctgtc caatcagaat cactgtcggt aaaaaagcag
     4741 tggatggaat cgtagaagtg aaaatcaaga aaactggcga aatggttgaa gtacgtaaag
     4801 aagaattggt gaatacttta ccaatcctct tgaaccaagg cgaataataa gcgttaaaac
     4861 acagaaaata aaaatagctg aatcagctgt ttttgacaat ctgatgattc gcttttaaaa
     4921 ttggaactgc gatttgaatc gcagttccaa ttttttaatg taaaactcag aaaaaacgag
     4981 tatactaaaa ggtactgaaa aagaagagga taagatgcga tcaattgcat tgtttctcat
     5041 cagaaagggc gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa
     5101 atccaactag aggaagcaga aaaaaatcat ccactgctta ctgctggtga aatggatagt
     5161 gtagtggtcc atcgtaaatc gcgtttatgg gaatttacgc tgcatttttc caaaattttg
     5221 cctattatgc tatatcggtc attgactcag catctgacta tcgcttttaa agatattgca
     5281 caagtcaaac tgaatatctt agcagatgac cagacattcg atgaacagct cttgcaagat
     5341 tactgggccc aagctttgga aaatcagcaa tgcgatacac cactagcaca gcaagtattg
     5401 aaaacgcagg tacctgtgat aaaagatcgt aaagtcatct tgccaatcga cagtacagga
     5461 gctatttctt atttgaagca acaatacttg ccattagtag aagagttgtt cgtcagttac
     5521 ggattcccaa aatttcgtat cgaaccagaa gtggatgagc agcaagcgga acgagtattg
     5581 aaactttttg aagaacgcaa acaagaacag gcagaagcct ttatgaagca agcagctgaa
     5641 agtctagttg tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa
     5701 ggacccatcc acatccagct aggacgaaat attccagccg atgaaccaac gacaccaatg
     5761 atcagtatcg ttgaagaaga acgaagagtc acacttgagg ggtacgtatt cgacaaagaa
     5821 gtcagagaac tacgttcaaa acgcaagatc ttgactttga aaatcacaga ttatacttca
     5881 tcatttatcg tcaaaaaatt ctcgaacggc gaaaaagatg aacaagtatt tgatgcgatc
     5941 agtgtcggta gctggttaaa ggtacgagga agtattcaag aagatacgtt tgtccgtgat
     6001 ttggtcatga atgcgcaaga tatcatagaa gtcaaacata cacctcgcaa agattatgct
     6061 cctgaagggg aaaaacgagt agagcttcat gttcatagca atatgagtac gatggatgca
     6121 acaaacagca tctctgatct tgtggcacaa gcagggaaat ggggacaccg agctatcgcc
     6181 attacagacc acggtggtgc gcaagccttt ccggaagcac atagtgctgg aaaaaaagca
     6241 ggtgtgaaaa tcttatacgg cgtggaagcg aatgtcgtag atgatggtgt cccaattgct
     6301 tataatgatg cacatgaagc attgagtgaa gccacatatg tcgtatttga cgtggaaacg
     6361 acaggattgt ctgcagtata tgacacgatc atcgagctag ccgcagtcaa aatgtataaa
     6421 ggaaatgtga tcgaaagctt tgatgaattt atcgatccag gacatccttt gtcacgaaca
     6481 acggtagatc tgactggtat cacggatgga atggttcgag gatcaaagtc ggaagaagaa
     6541 gtattgcgga tgttccttga attttcaaaa gatacaattt tggtagccca caacgcggct
     6601 tttgatatgg ggttcttgaa tactagctac gcaaggtatg gtattcctga agcggccaat
     6661 ccagtcattg atacgttgga attagctaga tatctgtatc ctcaattcaa acgtttcgga
     6721 ttaggtgtgt tatctaaaaa atttggtgtc agcttggaac agcatcaccg agcaatctat
     6781 gatgctgaag caactggtca cttagcatgg atctttgtga aagaggcaat ggacaatcat
     6841 aacatgcttt atcatgatca gctaaatgag catatcggag aaggagattc ttacaaaagg
     6901 gctcgacctt tccatgtaac gattttagca aaaaatcagg caggactgaa agatttgttc
     6961 aaactgattt caatgtcgaa tgtcgaatac ttcgaacgcg tcccacgaat accacgctcc
     7021 caattgaaaa agatgcgtga aaatctattg atcggctcag cttgtgataa gggagaaata
     7081 ttcgaagcca tgatgcaaaa aggggtagaa gaggcaagaa accgagcaaa attctacgat
     7141 tatattgaag ttatgccaaa agcggtctat gcgcctttga tcgagcaaga attggtaaaa
     7201 aatgaacatg atttggaaga aattatccaa aatttggtag aaatcggaaa aagtttggat
     7261 aaaatcgttg ttgcaacagg taatgtacat tatctgaacg aagaagatgc catttaccga
     7321 aaaattttga tcaattctat gggcggagcg aatccgttga atcgtcatag tttgccggat
     7381 gtccatttcc gaacaaccga tgagatgcta actgcatttc actttttagg ggaagaaaca
     7441 gcgaaagaga tcgtagtaga aaatacgaat aaaatcgcag atatctgcga agaagtgatt
     7501 ccagtcaaag atgaacttta tacaccgaag attccaggtt cagaagatga aatatcagaa
     7561 ttgagttaca caaaagcaaa acaaatgtac ggagatccgt tacctgagat cattcaaaaa
     7621 cgattaaaaa aagagttgaa ttctattaat ggtaatggct tctcggttat ttatctgatc
     7681 gcccaaaaac tagtgcataa aagtaatgaa gatggctatc tagtaggttc acgtggctct
     7741 gttggctcta gctttgttgc gacgatgaca ggaatcactg aagttaatcc gttagcccct
     7801 cattattatt gtccagagtg ccaatattcc gagttttttg aagatggtac gtatggttct
     7861 ggatttgaca tgccagaaaa acaatgtcca aaatgtggtg cacgcttaaa caaagacggt
     7921 catgatattc catttgagac atttttagga ttccatggag acaaagtgcc cgatattgat
     7981 ttgaacttct caggtgatta tcaagcagaa gcccataact atacgaaagt attgtttggc
     8041 gaagactatg tttaccgtgc cggaacgatt ggtacagtcg ccgacaaaac agcatatggc
     8101 tatgtaaaag gatacgaaag ggacaataat ctgcagtttc gaagtgcaga agtcgacaga
     8161 ttagcaaaag gcgctactgg agtcaaacgg acaaccggcc agcatccagg ggggatcatc
     8221 gttattcccg attatatgga tgtctatgat tttacgccga tccaatatcc agcagacgat
     8281 caaaattcgg aatggaaaac gactcatttt gattttcact cgattcacga taatgtattg
     8341 aaattagata ttctagggca tgatgaccca actgttattc ggatgcttca agatttatca
     8401 gggatcgatc cacaaacgat ccctacggat gacccggaag tgatgcggat atttgccggt
     8461 cctgaagtac ttggagtaag tcaggaacaa atttattcca aaacagggac attaggtatt
     8521 ccagaattcg gcactcgttt tgttcgaggg atgctagaag aaacgcatcc tacaactttt
     8581 gcagaactac tgcaaatttc cggactatct catggtacag acgtatggtt aggaaatgcg
     8641 gaagaattga ttcgacgagg agatgcgaca ctggcggaag taatcggctg tcgtgatgat
     8701 attatggttt atttgatcca tgctggatta gatagcggga tggcatttaa aatcatggaa
     8761 actgttcgta aaggacaatg gaacaagatc cctgatgaat tgcgagaaac ctatctatcg
     8821 gcgatgaaag aaaataatgt gcccgactgg tatatcgatt cttgttcaaa aatcaaatat
     8881 atgtttccga aagcccatgc tgcagcttac gtattgatgg ctcttcgtgt cgcttatttc
     8941 aaggtttatt tccctatttt gtattactgt gcatactttt ctgtacgtgc agacgacttt
     9001 gatctagtct ccatgtgcaa aggaaaagat gctgtgaaac aagcaatgaa agaaatcacg
     9061 gacaaaggat tagatgcttc cgtaaaagaa aagaatcaac tgacagtgct ggagttagcc
     9121 aatgaaatgc tggaacgagg attcaaattt ggaatgattg atttgtataa atcggatgcc
     9181 gttaactttg ttattgaagg tgatacgttg atcgcaccat ttagagctgt cccaagtttg
     9241 ggaaccaacg tggcaaaaca aatcgttgaa gcaagaaaag acggtccatt tttatccaaa
     9301 gaagatcttg ccacccgcgg taaagtttca aaaacattga tcgaatatat gaacgacaat
     9361 ggcgtattga aagatctacc agatgaaaat caattatcgt tattcgatat gctataagca
     9421 tctgttttcc tgtctgcttg taattttaag cagagtatgg tatagttctt ataggtaatg
     9481 atactaactg tgagtgagcg gatttttcgc tcactctttt taatggaatt acgcacttaa
     9541 ttttttaagg aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca
     9601 ccgattttag atgaacaaaa ttttgaactt gtcgaagtcg aatttgtaaa agaaggaaaa
     9661 agctggttct tacgagtatt tattgacaaa gaaggcggta tcgatatcga agaatgcgcc
     9721 tttgtcagtg agaaactgag tgaaaaactt gatgcagcag atccagatcc gattcctcaa
     9781 gcttatttcc tggaggtatc ctccccggga gcagagcggc cattgaaaaa agaaagcgac
     9841 tatgaaaagg cacttggtga ttatgtccat gtctccttat accaaccagt agatggcgaa
     9901 aaacaatatg aaggatttct tcaatcattt gatgctgaac agcttacatt gaaaatacgc
     9961 ataaaaacac gagaaaaaga attgactttt gaccgaaaaa atattgcaaa agctcgctta
    10021 gctatccaat tttaatcacg gagaggaatc agaaaaaatg agtaaagaaa tgttaaatgc
    10081 gttagacgct ttagaagctg aaaaagggat ctcaaaagaa atcgtgatcg atgctttaga
    10141 agcagcattg gtttctgcat acaaacgtca ctatggccaa gcacaaaacg tcgaagtgga
    10201 atttgatcag aaaaaaggaa agatccatgt ctatgcggta aaagaagtaa cggaagaagt
    10261 gatggattca cagttggaag tctcattgaa agatgcactt ttaatcaatc cagcatatga
    10321 gatcggtgat acgatccgtt ttgaagtaac accaaaagat tttgggcgga tcgctgcgca
    10381 aacggctaaa caagttattt tacagcgtgt acgtgaagca gaaagaacga ttatttataa
    10441 cgaattcagt gcttatgaaa aagacatcat gcaaggaatc gtcgaacgcc aggataaacg
    10501 ttatatttat gtcaatcttg gtaaaattga ggcagttctt tctaaacaag atcaaatgcc
    10561 aaatgaattt tatcaaccac atgatcgaat caaagtttat gtttcccgag tagaaaatac
    10621 gtctaaaggt ccgcaagtat ttgttagccg cagccatcca gacctattgc gccgcttatt
    10681 tgagcaagaa gtaccagaag tctatgatgg aatcgttgaa attgtcagca tcgcccgtga
    10741 agctggcgat cgttcaaaag tggcagttcg ctcaaatgat ccaaatatcg atgcagtcgg
    10801 tacatgtgtc gggccaaagg gacaacgagt tcaagcaatc gtcaatgaat taaaaggcga
    10861 aaacatggat atcgtcgaat ggaatgaaga tccagctgta ttcattgcaa atgcattgaa
    10921 tcctgcacaa gtcatggatg tgatcttcga tgagacaaat ccaaaggcct gcacagttgt
    10981 cgttccagac taccaattat cgctagcaat cggtaaacgc ggacaaaacg cacgtttagc
    11041 tgctaaattg actaaccata aaatcgatat caaatcagaa tctgacatga cggaatttta
    11101 tgaaaatcaa gaacaacaaa aagaaacaga agaactgcat gatgaagcaa tcgttcagtc
    11161 agatttaaca gacgatgaat atgaaacaat tgcattcaac aatgaaacgg tcgaagaaaa
    11221 accagaagct taactttagt aaggaggcaa agagatgaaa caaaggaaaa ttcctttacg
    11281 caaatccgtg gtttcaggtg aaatgaaacc taaaaaagag atggtccgaa tcacccgttc
    11341 aaaagaaggg gaagtatcga tcgatccaac tggaaaaatg cctggacgag gagcgtatgt
    11401 ctcacttgag ccggaagaag tacaacaagc ttgggataaa aaaattctcg atcgagtact
    11461 cgaaacaaca ttaacagatg agttttatca agaacttctt gactatgtca ctcaccaaaa
    11521 agcacgtaaa gagttatttg gaaaatgaat acagttaatc ggcaaaaagc catgaatctc
    11581 atcggtctag cgatgcgcgc gggaaaacta atcacgggtg aagaactgac cattgctgaa
    11641 atccgcaaac aaaaagcaaa attggtgttt gtggcaaccg atgcaagcga gaatacaaga
    11701 aaaaagataa aagataagag ttcatattat gaagttcctt gctttgagct attttctgaa
    11761 gcagaaatca cgcagatgat cggaaaacct cgcaaggtat tcggaataat ggatgccgga
    11821 tttgcgaaga agaccaagga gctaattgaa ggttaggaag gtgattgcat gggcaacaaa
    11881 agaatctatg aattagcaaa agaactcaaa aaatctagta aagacgtcgt ggaaaaagca
    11941 caacaacttg gcatcgatgt gaaaaaccat atgggtgcga ttactagcaa tgatgagaag
    12001 aaattgcaac aagcgttcaa aaacccaagc acgaaacaac aagctgcaca acaagcaagc
    12061 agaaaaccag ccaatcaaca gccgaacgat caacgtaaaa aagaaacaac gaacaatcaa
    12121 caaaaaaata aaaataaccg cagttatcaa gatcgcggtc aagggagcgg tcaagtgaat
    12181 caaggaaaaa aaccaacaac aaaccaaaat aatacgggaa ataccagcag ccaaaatcgt
    12241 caaggaacga gccaaggcgg caatcaaaac cgtactaaca ataacaacaa accaaacaat
    12301 aacaatcgcg gcaaatttaa taataataac cgtaatcgtt ataataaaaa aggtaaaaaa
    12361 ggcaaacagc aacaatcaac taaaccagct gtaccaccac gcaaattccg tgaattgcca
    12421 gatgtattag agtatactga aggtatgaac gtagcagata ttgcgaaaaa aattcatcgt
    12481 gagccagcag aaatcattaa aaaattattt atgcttggtg tcatggtcaa tcaaaaccaa
    12541 gcactggata aagatacgat tgaactgtta gcgacggact atggcatgga accacaagaa
    12601 aaagtccaag ttgacattgc tgatatcgat aaattcttcg aggcagaaga agtcgatcca
    12661 gataaattag tctcacgtcc gccagtcgta acgattatgg gacacgtaga ccatgggaaa
    12721 acaactttat tggatacatt acgccattca cgtgtaacaa gcggcgaagc cggcggaatc
    12781 acacagcata tcggtgccta ccagatcgat attgatggta aaccaatcac tttcttggat
    12841 acaccaggac atgcggcctt tacaagtatg cgtgcacgtg gagcaagtat cacagatatc
    12901 acgatcttag ttgttgctgc agatgatggc gtcatgccac aaacagttga agcaatcaac
    12961 catgccaaag ctgcaggcgt tccaatcatc gtcgctgtaa ataaaattga taaaccaggt
    13021 gcaaaccctc aacatgttat gcaagaatta agtgagtatg agttaattcc tgaagcatgg
    13081 ggtggagaaa cgatctttgt tgagatctca gcgaaattcg gccaaaacat cgaagaattg
    13141 ctggaaatga tcctgttagt cgcagaagta gaagatttaa aagcagatcc aacacaacgc
    13201 gcaatcggaa cagtgatcga agcacgtctt gataaaggta aaggtcctgt gtcaacctta
    13261 cttgtccaag aaggaacact tcatgttggt gacccaatcg ttgtcggtaa tacttacgga
    13321 cgtgtccgtg taatgacaaa cgatcttggc cgtcgcgata aagaagcagg acctgcaacg
    13381 ccagtagaaa tcactggctt gaatgatgta cctcaagcag gtgaccgttt cgtagtcttc
    13441 gaagacgaaa agacagcacg tgcagcgggt gaagaacgcg cgaaacgtgc attattagaa
    13501 aaccgtgctg ccacaagccg tgtgactttg gataacttat ttgaaagcct gaaagaaggc
    13561 gaattgaaag aagttaacgt aatcatcaaa gcagatgtac aaggttcagc agaagcactg
    13621 gctgcatcat tgaagaagat tgatgtggaa ggtgtccgtg taaaaatcgt ccattcagct
    13681 gtcggagcaa tcaatgaaag tgatgtaacc ttagcagcag ccagcaacgc tattatcatc
    13741 ggtttcaacg ttcgtcctac accgcaggca aaagtgcaag ctgatactga agaagtcgat
    13801 attcgtcttc accgtatcat ctataaagcc atcgaagaaa tcgaaactgc gatgaaaggg
    13861 atgctagacc ctgaatttga agaaaaaatc accggtcaaa tgatcgttcg tgaaaccttc
    13921 aaagtttcta aagttggtac gattgcaggt gcctatgtaa cagaaggata tattcgtaga
    13981 gatagcggtg tccgtgtaat ccgtgacgga atcgtaatct atgaaggcca acttgcaagt
    14041 ctgaaacgat tcaaagatga cgtgaaagaa gtcaaaatgg gttatgaatg tggtgcaatg
    14101 attgaaaaat tcaatgacat caaagtagat gacgtaattg aaggatttat catggaagaa
    14161 gtcaaaaatg actaaaaact gataaaagga ggccaatcat tatggctaac taccgtgacc
    14221 gccgagtcgg tcaagaaatc ttaaaagaag tcaatgatat tttgcgcaaa aaggtccgtg
    14281 accctcgtgt ggagaacgta acgatcacag atgtgaaagt tacgggggac ttacagcaag
    14341 caacgatcta ttacagcatt ttatctgact tagcatcaga taagaaaaca gcacaagatg
    14401 gattgaataa agcatcagga ttgattcgtc gagagttagg gcacaatcta agtatttata
    14461 agacacctga attgttcttt gaacttgatg aatcagttgt ctatggcaac catatcgatg
    14521 aattgcttcg cgacttgaac aaagactaat caaaaactcc cgcaaatcct gtgaaatcag
    14581 gggttgcggg atttttttgt tttcattaag acgtattacc taagagttcc atgactttag
    14641 tcttgcggcg tgtcagctaa ccaaaaaaga gaaaaagaaa agtaaaaaat tcgactttct
    14701 tttttctagt agtatataaa tttttgcata tagataaaga tcaagatacc gataacggaa
    14761 gctagattgt aatctatttt agtgacagtc aggtatagcc aagtaactag aagatcagtg
    14821 gaacctgcac cataaaagtt tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg
    14881 ttgaagttat tgatatttcc gattacgccg cttcgatttg gtcacgatcc aaattttgga
    14941 taatccctgt agaaattagc atagtagctg ggataccaat tcacatattg acaaagataa
    15001 cagagacttt tacataaaac ggatcaatca ggaaaggaat tgctcgatcc agccccagtt
    15061 ttccagcaat gtgttaactg gcccagcgtc attcagcaaa ttatgcatga tgaggagcga
    15121 aatgaactgc ggaacagcca ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg
    15181 tcagcgaaag gaaaaatttc gtggtttttt atacctgttc atttggctag atataaaatc
    15241 caaacaaatg aacagaagtg cttattaggt ttcaattggg agtatctgcg ctagaataag
    15301 agtaataatg aaaaggagtg ttatgtatgg attattctag taaagaatac tttgataaaa
    15361 tgacggcttg gtggcgtgct gctaattatc tttctgttgg gcaactttat cttaaagata
    15421 accccttact gagacgtaca ctgaaacccg aagatgtcaa aaaacatcca atcggtcatt
    15481 ggggaacgat tcctggccaa aattttattt atgtccattt gaaccgagtc atcaataaat
    15541 atgatttgaa catgttttat attgaagggc caggacacgg cggacaagtc atggtatcga
    15601 atgcttattt agatggaagt tacacagaga tctatccaga agtaacggaa gatgagacag
    15661 gaatgcaaaa attattcaaa cgattttcat ttcctggagg cattgcttct catgcagcac
    15721 cagaaacacc aggttctatc catgaaggcg gagagcttgg atactctctt tcacatgcag
    15781 tcggagctgt attagacaat cctgaagtaa tctctgctgt cgtgattggt gatggtgaag
    15841 cagaaaccgg tcctttagct ggaagctggt tctcaaatgt ctttatcaat ccagtgatag
    15901 atggagctgt tttaccgatc cttcatttaa atggtgcaaa aatagccaac ccaacgatct
    15961 tggcacgtaa atcagatgga gaattagcaa attactttaa cggacttggc tgggaacctt
    16021 tctttattga aggaaatgat tcagaaaaat taaatcctgt tatggctgaa aaaatggatc
    16081 aagcaatcga gaaaattaaa tccatccaaa aagaagctcg cctgaagaca gcaacagatg
    16141 tcgtgatgcc aaaatggcca gtattgatcg tacgcacacc aaaaggatgg accggtccaa
    16201 aagaatggga cggggaacca attgaaggaa ccttccgagc acaccaagta ccgattcctg
    16261 tagatcaaga acatatggat catgcagatg ctttactccg ttggctgaaa tcctatgaac
    16321 cagaaaaatt atttgatgca caaggacgta ttttagaaga aatcagagaa atcgcgccta
    16381 ctggtgacca aagaatggca aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc
    16441 ttattatgcc agattggaaa aaatatactt tgcaatttga aaaaccaggc tccgttaaag
    16501 cagaagatat gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa
    16561 atttccgtat atttggacca gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa
    16621 cgacaaatcg ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat
    16681 ctggtcgcgt catcgacagt caattatcgg aacaccaaga tgaagggttc ttagaagggt
    16741 acgttctgac tggacggcat ggttttttgc aagttacgaa tcattcttgc gagtggttga
    16801 ctctatgttg actcagcact tcaaatggat gcggaaatca catgaccttt cttggcgaaa
    16861 tgattatcct tcgctaaatc tcattgcaag ctcaacagtc ttccagcaag atcacaatgg
    16921 atatagccac caagatccag gaatattgac acatttggca gaaaagaagg cggagttcat
    16981 ccgtgaatat ctgcctgcgg atgcaaatac attgctcgca gtcatggata aagcattccg
    17041 ctcgtcagaa aaaatcaatt tgatcatctc ttccaaacat ccgcgtgctc aattttactc
    17101 agcagaagaa gcagccgtct tagtgaatga aggcttgaaa atcattgatt gggcttcgac
    17161 agcaaaagag gaggaacctg aattggtcat tgctgctgca ggaacggaat ctaacttaga
    17221 agcattagca gctgtaactt tgctgctgga agaattccca aaactaaaaa ttcggttcat
    17281 caatgtcgta gatttattga aattacgtca tccaagtcaa gatccgcgtg ggttgagcga
    17341 tgaagaattt gatcaatatt ttacaaaaga caaaccgatc ttatttgctt tccacggtta
    17401 tgaaacattg gtccgcacga ttttctttga tcgtcataac catcatttaa tgattcatgg
    17461 ctacaaagaa aatggagata tcacgacacc atttgacatg cgtgtagtaa atgaacttga
    17521 tcgttatcat ttggcaaaag acgcagcttt aaaaatcaaa ggaagtcaag cagaagattt
    17581 tgcgaaaaaa atggatcaga aattacaaga acatcaaaac tatattcgtg aaaatggtat
    17641 tgatctgcca gaagtattgg attggaaatg gaaaaatctt gaccaataaa atgataaaat
    17701 cgatcccgca aaatagaatt ttttacattg attaagaaac tgaaaaaata aataaaaaaa
    17761 acggaaaaaa gactggaaga atggccagtc ttttttttcg tttttgggaa gtgaaaacca
    17821 aactatttac ctgtaagcca cccataaatc caatcaaatg gtgcaaagaa aaaatcttcc
    17881 attctattcc ctcctttaaa aaagcataac atgatttcta tttacgtgag catgaatttt
    17941 gttacgtttt tattgccgaa aaataaaaac cgttcattat gatgtcaaat tcgtcagtaa
    18001 ttgatgcaat gtgttataat aattcagtta aatcaaaaat gaaatggaga aaagacatgg
    18061 acgggttatt acctttatgg aaagaacgcg gaatgacaag tcatgattgc gtattcaaat
    18121 taagaaaaat cttacatacg aaaaaaatcg gacatggggg tacgcttgac ccagacgtag
    18181 acggtgtgtt gcccatttgt attggaaaag ccacaaaagt tatcgaatat ttgacagatt
    18241 ctgggaaaat ctatgtaggc gagataactc taggtttcag tacaacgacg gaagatcgat
    18301 ccggagaacg ggtagaaaca aaaccagtag atatgtcttt atctgaaaaa aaaatagatg
    18361 aagtgatggc ttcatttatt ggtgagatca cccagatacc accaatgtac tcagcggtaa
    18421 aagtcaacgg taagcggtta tatgaatatg cccgcaataa tgaaacagtg gagcgtcctg
    18481 ttagaaaggc tcagatttac cgttttatcc ggacaagtga attgacaaga gacgaagtga
    18541 caggaaccat ttcttggcgc ttcgaagtag aatgcgggaa aggaacctat gtccgcacat
    18601 tagctgtaga tacaggcaag aaacttggtt accctgcgca catgtctgat ctgacccgaa
    18661 cagcaagtgc cggtatgtcc cagaaggatg ctctgacatt agaagaagta gctcaagctg
    18721 tgaaaaatgg gacaatcgat gattgtttgc tgccaataga aaaaggagtg gaacggttca
    18781 aacgagtaga tattactgat gaagtatggc agaaagtaaa aaacggtatg agacttgatt
    18841 atactgtatt tgcgctccca gaaatgccgt cagaagaaat tgctttgttt tataaaggaa
    18901 aagttgtcag tatttatcag ccaaacccga aagaaaaaaa caaattaaaa cctagtaaag
    18961 tattgagaaa tgaggaataa cgaatgaata tcatcaagat cagacatcct taccaatcag
    19021 aacaaattcc agaagacgat gtcgttttag tgctgggatt tttcgacggc gtgcatatcg
    19081 gacaccagaa agtcataaag acaggtatgg aaattgcaca aaaagaagga ttgaaattag
    19141 cattgatgac tttcaatcag catccatcta ttgtctttaa aaagatcgat cctagcagcg
    19201 tgaaatattt gactagcctt aagcaaaaag aagaaaaaat ggccgctttg ggtatagatt
    19261 atttatatga agttgatttt acttcttctt ttgctcattt agcaccgcag gaatttgttg
    19321 atcaatacat cgtagggtta catgcaaaat atgctgtttc cggttttgat tatacgtatg
    19381 gaccaaaaga cattgcagat gtcgctcatt ttcctgaata tgccaaagga agattcaaaa
    19441 tcgtcacagt ttctaaagaa gaagaacaag gtgaaaaaat cagctctacc cgtattcgtg
    19501 gattactaga cagcggacaa gtaaaagaag cagcaaaact attaggttgc acatatgagg
    19561 tagaaggggt agttgtccat ggcgatgcac gaggcagact actaggattc cctacagcaa
    19621 atgtcaaagt aaagagcacg gttcatttgc ctaaagaagg tgtttatgtg tcggaaataa
    19681 aaattgggga aacatggtat ccagccatgg gttcaattgg acataatgat acattcggtg
    19741 aaggacggga gctgactgta gagttgtata ttttggactt taatcaagat atctatggtg
    19801 aacatgtcgc ggtacgctgg aatcaatttt taagagatca agtgaaattt tctagtgtag
    19861 aggaattgat tgaacaactc aaggaggatg aacaagcaac agctgaatat ttttctcagc
    19921 aataaaacat tatgcataaa atctcttgtt tatgtataac tagcaattac aataaaatgg
    19981 gtgataaata agcccaggaa aggggctgtg acaaaagtct tgtcacagcc tttttttcca
    20041 aataaatggt ttttcagaag tagcttcttc ggaaataagc cgaaatttct tgaagctgac
    20101 cacttctgtc acaacctctt tctttataga aataaaagga cactagcaat tcatgaaaac
    20161 gattgctaga gcgtatcatt tgctttagac tatatatctt ggtgtacact cactttaagg
    20221 cggaaataaa agttatccgt aataacaggt ttaggtatga aaaaggagtg ggtactatgt
    20281 acaaggaatt agaaaacaaa gtcgcagtag taactggtgg ctcaaagggg attggaacag
    20341 cgattgctca acgattcggg aaagaaaaaa tgaatgtagt tgtcaactat aactcggata
    20401 aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc aggcggaaaa gcagtctctg
    20461 tacaagcaga tgtcagttct gaagaaggaa tacagcgttt acttgatacg gctttagaaa
    20521 attttgggac actggacgtt tgggttaata atgctgggat ggaaaatcag catccaaccc
    20581 atgaacttag ccttgaagat tgggaaagag tgttaaaagt caatttgact ggcgttttta
    20641 tcggaacgaa agcggcttta aattacttta cagagaataa taaaaaagga aatatcatca
    20701 atatgtcttc tgttcatgaa cagatcccat ggccaacgtt tgctcattac gctgcttcaa
    20761 aaggcggggt aaaattattt accgaaacgg tagcaatgga gtatgcaccg aagggaatca
    20821 gaatcaatag tataggccct ggtgctatcc gtacacctat taatgcggca aaatttgatg
    20881 atcctgaaca aaaagaactc ttggaaagta tgatcccact aggacgcgta ggtgaaccaa
    20941 aagaaatagc ggcgacagct gcatggttgg cttcagacga atccagttat gttacaggta
    21001 ttacactatt tgcagatggt gggatgactt tatatccttc tttccaaggt ggaaaaggat
    21061 aagggtgaat aaaaatgggt ttattgattg cattgatccc ggcagttggc tgggggatcc
    21121 agccactgat tttgaaaaag attggtggca gaccaacaaa tgagatatta ggaactggta
    21181 ttggagcggt gcttgtcggc ttactcgttc aattattcgc ttctccagct ggtatttcag
    21241 tgactacatt tttgattagt ttactttctg gtgcattttg ggttattgga caaacaggac
    21301 agtatacatc tttcaacttg attggtgttt ctaaaacgat gccaatttcg actgcattac
    21361 agttagtcgg aacctcgttg attggtgtct tcgcttttgg agaatggtct ggaagtatgg
    21421 ggaaaatcat tggagcagca gcaatcattc ttcttgtatt tggttctgct ttaacggcaa
    21481 tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg aattagtcta ttagcatcaa
    21541 caagtattgg ttattgggta tacagtgcat tgcctaaact agttgatgca agcggacttt
    21601 ccattttctt tccgcaaatg atcggtgtct ttttaggggc agtgatttat gtgattttcc
    21661 gtaaaccaac cgcattttct gaagacaaga gttggaaagc aacgatcgtt ggagccatct
    21721 ttagtgtgtc agcattggct tatatttttt cagcaaatga aaatggggtt gctactgcat
    21781 atatcattac acagctaaac gtagttatct ctacattagg cggtatgtac atcctgcatg
    21841 aaaataagac accaaaagaa aaaaaattaa cgattatcgg attattgctg atcgtagtag
    21901 gaagcatggt cacggtcttt atttgaggat aaagatggta aaatttcatt tattaaaaaa
    21961 atatttgttt gaaccaaaca gtaagtactt ataaaaaaat agcgctagaa tttccaacat
    22021 ctctggaatt tctagcgctt tttaattgtt taagatacaa gggaaatatc tgtctggatc
    22081 aataattttt cattgatcca ttgactaaag tttacgacat aattgaagtt accaccgatt
    22141 agtatctccg catgattgtc tttataaaga tcgtattgta aaagacggtc ttttttattt
    22201 gcgtaaacat cgttatggta ctggactaat tgttctcgga attgattgat tgctgtaaaa
    22261 tcacgatatt gaaggttgaa acgcatcgtg attggtgcat ttagcatgaa tcgctgcatt
    22321 tcttttgctg cttcctctgc ttgatctccg tataggaact caaaaagatt acgttcagaa
    22381 aatttccatt gcccgccaac ctttgttcct tttagttttc cttcatgaag ataatttctg
    22441 atagaacgtg tagtaacacc taattgttcg gctaattcat ttactgagta aaaatctttc
    22501 aatgttcacc ctcctatgtg aatggttcta ctgacttagt tgatatttgc ttaatcacga
    22561 aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa tcaagtcaga aaattctaac
    22621 ttttatttct ctaaagaaat tcgttctctc ctaaagtgat attatattta aatatacctg
    22681 aaaataatgg gcagtattat gaaagatcac tgatattttc tcgtattttt tccttattca
    22741 aaagttttgc aggaaaattg ctgaaaaaat aattttacta agtagaaaat tcaaagccat
    22801 tcaatcagtg atttttttca gcacaagaaa gactccttat aaaaatagca gatactttgt
    22861 caattaagtg tttaaccagg gatgataaaa aggataggat tgaacaaaac ctgtataagg
    22921 tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat aaagctttat cgccaaaatt
    22981 tatcatgacc ttttttatct tcaatattaa atgaaatcaa ttgttcaatc aattcccagt
    23041 tgattacact tttccaagga actttgatga cgttatccgt atatacgtag cctgcttctt
    23101 ggatattttt ttcaagggca cgaatcgctg ctttttccgg tgcgatagaa aaatgatttt
    23161 tagcaaaact aaatgcaata ataaaggtac catggtcagt aaacataggt tggttccatt
    23221 ttacagttgt gtctagctgg ggatacgttt ctgttatctt agcaaaaagg tctttgatct
    23281 tttgttgatg ttctttttct ttgatttgat tgatgatcgg ctgaaatttt tccattctaa
    23341 aacgcctcca ttaagtggtt tgatttattt taccagaaaa acaatagtta tgtataaaaa
    23401 acgaacaaaa cacaagtaat gttgtaaccg ctatatttat atgataagat gtctcaaata
    23461 ataaaataga aaagagacat aaaaatgatt gacttggcaa agaaattctt acacgaaaat
    23521 atatattttg agttgtatca tcatcgagcc atttatacaa atgaagatgc tttggtaata
    23581 aagaaagaac aaggatttac aggtacagag accaaaagct tattcttaaa agataaaaaa
    23641 gataataact acgtatatct gacttttact acgaaacaaa cggatttcaa agcattaggg
    23701 aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac gcatggaaga acaaacggga
    23761 caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac agattccgat tattattgat
    23821 gaagagctct tatcaaatga aaaacttgtt tttgcaccag gtagaccaga tcaaacgatg
    23881 gtcatccaaa cggttgactt aaacaaaatc atccagctac ttgagatgcc gatatttttg
    23941 atgccggcaa acgaaaaaaa caattaacca agtaaaatag tgcatacttg gaggtgaagc
    24001 aacatgataa gttatccatt attagaaaga aatgcaacga ttggggttac tgcaccatcc
    24061 tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag ctttaaagag aatggaagaa
    24121 aaatctttcc aagtagttac tggaaagacg ctttggacgc aagataagga aaaatcaggt
    24181 ccagcaagaa aacgagcaga tgaattcaat caaatgatgc aagatgaaaa tatccaaatg
    24241 atctttccga cttggggagg agaattattg attgagattc tcgattttgt cgattttgat
    24301 gctctacaac caaaatggat agtaggttat tcagatatta gtacgttgct acttgctgtc
    24361 acattaaaaa cagggatagc cactgcacat ggaactaacc ttattgattt aagaggagag
    24421 tacttggatg aaacgacagc aatgtgggaa agtgtactga aaacaagaga aggagaatcg
    24481 attctacagc aatcttctga aaagtatcaa aaagtgtggc cccatgagca ttcatcatct
    24541 tctgttttcc atctcactga accaacaaat tggcaatcag tcagcggaga tgcagtaagg
    24601 atgaagggac gattattagg tggttgtatt gatacgatca ggcatttaat cggtactcct
    24661 tatggagatg tccaatcttt taaaagaaag tataatgatg gaaagccgat cttgtggttt
    24721 ctagaaaact gtgagctatc gtcagttgat ttaagaagat cacttgttca gatgaaactg
    24781 gcaggctggt ttgatggcag ctcggggatc atgttcggta gaagtgcggc agaggatttg
    24841 ttagattata aaatgaagga tgtatacgaa gatctgtcaa aggaattaaa actaccgatc
    24901 ttgtatgata ttgattgtgg tcatgtaccg ccacaactga cattgattaa tggtgcctat
    24961 gcagaagtag cggtagaaga tagaaaagcg actgttttgc aaacgtttat ttaaaaaatt
    25021 ttttcctgcc ttggagtcta tcaaagacca ataaatctaa atagaaaaga aagtgaaaga
    25081 agctgacaaa atgctgtcag cttctttttg tataacatat tagaaagtct aattatctat
    25141 aactgtcaca tcgattaact gtttcacgtc attgggattt cctgtaggaa tagctactac
    25201 agatgcatta tcaatgattt cttgagttgt ttctctaatg aacgctggat aagatgtttg
    25261 atctgcaggc atttgccaat gtgaaggaac tggataacta gtgattgtcc cgtcatggtt
    25321 acttgaatac actaaaactc cctggtaact gtatttccca gtgagcataa ccacatcttt
    25381 cggatagact gcactccctg catcataagg ggcgataggt gttcctgcag gataatgaat
    25441 aacatgtaat tcgaaatcac ccgatgtatc tctaaaagta ttacctctaa tagctagcca
    25501 aacacgtgcg tactcaattt gtagatcaga atatcctgct aacggatcta ttgactgaga
    25561 agactgtgag gaggttgtgt ctattgttga agaagaaata tttgtctcaa ctgtactact
    25621 agatgtagaa gaagtattgg gctctgagac tttactgctt gttgaagatg tattcgtttc
    25681 agtagaagct gatttctgag tacttgattt ttcagctgaa ctggtttgtc tgattgaact
    25741 tttgtctgtg gtcgcagatg gatcactatt ttctttacta ggagacgtac atcctacaag
    25801 gaataaagag accaacaata aaataaaacc taactttttc atataaaata cctcattatt
    25861 tttttaatac cagtatagca taatcctgaa tttttgaatc cgtaaaacct tgtctatgaa
    25921 tataaataga gtgtatcttc tttgataaat gtcagaaaaa gtgtgacaaa taatcatcag
    25981 taaaattact tgtcacacta ttgataagga gtatttaatt gaagagaatg attaggagaa
    26041 aggatctccg tactttttat agtaatcata gaatccaagt ttgtatagac gaatcgtttt
    26101 ttctcttatt tcttctgttt caaaatggac tgcttgaaga atcaagtctg tgaattctat
    26161 aggagctgtt ccattcgcag taatcaaatt acgatctttc acagcttgtt gttctataaa
    26221 atcttcttta ttctgataat tttccgattc taaccaagga aattgcgcat ttccggtgtg
    26281 tttgtaacct tgcaataatc cgctctgagc taaataatca actgccccac aaatagcagc
    26341 tactggtacg tctgttttta acaatttatc gattatttga tataaatgat gattttttat
    26401 gtcccatgaa ttgccaccga tcataataaa aaggtcaact ttttcaggta tttgttcgat
    26461 taagtaatcg atttttgttt ttaatcctcc aatggaagtg acctctgatt ggatagaagc
    26521 agttttgttt tgccatttct cattttggtt tagtgtgcta gtaatataag caccttccca
    26581 atcggcatat tcctctaata gaaacgtaat cgcagttttc attttcaagc tctcctttac
    26641 tatctataac aaggacaaca gttttaacct acagacattc attaaaattg ggtagcgaca
    26701 gcgaaaacaa caattcttaa gttttagaca gagtatacag atagtcaact taattaaata
    26761 cgaactaaat aaaaatgaca ggtaatcatt tttgtttacc agcattataa cattttgcat
    26821 acatggatga caaaagaacc aattcccact tgcttgagta caatattaaa agaaactgga
    26881 aggcaaaaag taattataga aacgaaaaat aaatcatatc gggcactcag acatgaaaac
    26941 aaccctagag atatataact aggtaaccag tttaatgaaa gaaaaaatca tccaagaagt
    27001 cgattcttgg attttttagt ggatttgttc gccaaatacg tttagtatgc gtatatgaga
    27061 ggttgtaagc aaagcgttgt ggtctgagca cttaaggagg aatctgtgaa aatagctttt
    27121 catatttttg cagattttga cttaatgccg aaggtcagct ttgcgattac cgtttatcga
    27181 gaggctgtaa aagcaccgtg tagctccgat gacaataagc aagcattttt agagctaggt
    27241 ttccttgatt ctaaatatgc ggcttatttc aatgagttgg tgcttgaacg cgattttaag
    27301 aaggtcgaaa acaggcacga cttttcgacg taaggagaaa gaatttcaat gatagaaaca
    27361 ataaatcccg cagaaaacaa ggtttctgcg tacattcata tcccattttg cgagcatatc
    27421 tgctattact gtgattttaa taaagttttt ttagaagggc agccagtaga tgagtatgtg
    27481 gaaatgctgc tgaatgaaat gaaaataatg ctagaaagtc atccgacagc gaatttagaa
    27541 acactttacg tagggggcgg gacaccaact tctttatctg ctaaacaact tgatcgactg
    27601 ttatcaggtg ctagagagat cctgcctttt caaaatggta atgagtttac tgtagaagcg
    27661 aatccaggtg atttgacccg tgaaaaactt caagtaatga aaaactatgg cgtcaatcgt
    27721 ttatccatgg gagtgcagac atttgataat cgcttattga aaaaaattgg acgtaaacac
    27781 accgcagaag atgtgtatca aactatgcaa tttttagaag cagaaaattt taccaatgta
    27841 agcatcgatt tgatttatgc attaccggga caaacactag aaggatatct ggatactttg
    27901 gaaaaagctt tggctttgga tctgcctcac tattctttgt actcattgat cctcgaaaac
    27961 aaaacaatgt tcatgaactg ggtacgacaa ggaagactcg aactgcctga tcaggaaacg
    28021 gaaaccagaa tgtttgaaga gacgattgaa gcaatggaaa agcatggacg tcaccaatat
    28081 gaaataagta actttgggct ctccggacat gaatctcaac ataacctcat gtactggaac
    28141 aatgcccact attttgggtt tggagctggt gctagcggtt atcttggaga tattcgttac
    28201 cgcaacaaag gaccaatcca acactattta cgcccattac gagcaaatga acgcccggta
    28261 ttggaagaag aaaaattgac acgaaaaaat caaatagaag aagaaatgtt cttaggacta
    28321 agaaaaaaaa cagggattac taaaaaacat ttctatgatc gctatcaaaa aacaattgaa
    28381 tcggtgtacg gtgatgtgct aagggaatta gagcaagaag ggttacttgt taatgaagaa
    28441 gaccgtgttt atttgacacc aagagggacc tttttgggga acaatgtgtt tgagcgcttt
    28501 ttgtttgaaa aatagtttaa agaatatttg ttttttatct gaatttaaat aaaaaaagtt
    28561 caaaaataca ataaaggact attgaaacgt tgatataact ggatttacat caattattta
    28621 gaattagcac tcttgtgttt agagtgctaa aaaatggagt ctttctcttg acaggagaag
    28681 ggcttcttgc tatatttata agtggattag cacttagaat atatgagtgc taatgagagg
    28741 tgaggaagat gttaacaaag cgacaaaacg acattttacg tctgatcatc caacactata
    28801 cgagtagtgg cgtgcccgtt ggttccaaaa cattgatgtc tgagggagtc aaggccagct
    28861 cagcaacgat tcgcaatgat atgaaagcat tagaagatga aggattgctg ttgaagactc
    28921 actcttcttc tgggcggatt ccatctgctt taggctatcg ttattacgtg gatcatttgt
    28981 taaaacctgc tcgcgtagca gaagatgatt tgctgctgat tcgtcagtct ctaggcagag
    29041 aatttcatga aatcaatgag attattaagc aatctgctga gatattatct gagctgacaa
    29101 gctatacgac tttttcattg ggacctgaaa taaaagaccg ccgactgact ggctttcgta
    29161 tcgtaccctt gaataatcga cagattattg ctatcgtcgt aacagatatg gggaatgtag
    29221 aaagccaagt gttcactctt cccgaatata tgggaagtca ggaactagaa aaaatggtac
    29281 agattgtaaa cgaccgtcta gtcggagatc ctctgatgac ggtgtaccat aaacttcgta
    29341 cggagattcc gatgattctt cataaatatt tccaaacgac agaagggatg tctcagttat
    29401 ttgacaccgt tcttggtcat gtttttgaag ataaggttta tgtcagtgga caaatgaatc
    29461 tcttggatta tgagccccgt caagatcttg atcagttcaa atcgatgttt tcttttatga
    29521 aagactcaga cggacttaca caaatgatca ctccgatgga tagcaatatt catatcaaga
    29581 ttggtgctga attaggcaat gatttattgc agaatatgag tatgattcaa gcaagttacg
    29641 agatagatgg tcatggaaga ggtacaatag ccttgttagg gcctactagt atgccttatt
    29701 ctcggatgtt cggtctagtc gatgtttatc gaagagaact agcagctaaa cttgctgatt
    29761 actaccgttc gcttgacttg tcaaactcgt aatgtgaata acgaagctat ttgcagctga
    29821 tcgttctctc tttcttagta agagcaatca aacagctgac tgaataatga aaggaagact
    29881 gtatcgtgaa agaaaatcaa gaagaactag ataaagaaat gacagatgct cagccagaac
    29941 cggaaatcga tgtcgaagct gcagaagatt cgggtatttc agaagctgaa gcggaagaat
    30001 tcgaaacagc gaaattaaaa gctgagttag aagaaatgga agataaatac ttgcgggctc
    30061 gtgctgaaat cgcaaatatg gcaaatcgag gaaaaaatga gcgtgagcaa ttacaaaaat
    30121 accgttcaca agatttagcc aaaaaattac ttccatcaat tgacaacctg gaacgtgcat
    30181 tagccacaga agtgtcagat gatcaagggg caggtttgaa aaaaggcgtg gaaatggtct
    30241 tggaaagttt gcgtaacgca ctggaagaag aaggaattga aaaaatccct gctaaaggag
    30301 aagcctttga tcctaatctt catcaagcag tacaaactgt tccagcgact gaagatacac
    30361 cagccgatac gattgtagaa gtactacaag aaggctacaa attacatgat cgcgtgttaa
    30421 gaccaaccat ggtcattgtt gcacaataat tgtgcggata taaaacttac actttcaatt
    30481 aaagaaggag acgttaatta tgagtaaaat tatcggtatt gacttaggta cgactaactc
    30541 tgcagtagca gtattggaag gaaacgaagc aaaaatcatc gctaacccag aaggaaaccg
    30601 tacaacacct tctgttgtat cattcaaaaa tggagaaatc caagtagggg aagttgcaaa
    30661 acgtcaagct gtgacaaacc caaatacgat ttcatcaatc aaacgccata tcggtgaagc
    30721 aggttacaaa gtagatgtag aaggtaaatc atatacacca caagaaatct cagcaatgat
    30781 ccttcaatat atcaaaggat tcgcagaaga ctatttaggt gaaaaagtcg aaaaagcagt
    30841 tatcactgtg ccagcttact ttaatgatgc acaacgtcaa gcaacaaaag atgctggtaa
    30901 gatcgctggt ctagaagtag aacgtatcgt caatgaacca actgcagctg ctcttgccta
    30961 tggtttagat aaaacagatc gcgacgaaaa aatcttagta ttcgaccttg gtggcggtac
    31021 ttttgacgta tctatccttg aactaggaga cggtgtcttt gatgtattgt caacagctgg
    31081 cgacaaccat ctaggtgggg acgactttga taacaaaatc atcgactaca tggtagcaga
    31141 attcaaaaaa gaaaacagca ttgacttgtc tcaagacaaa atggctttac aacgcttgaa
    31201 agacgctgct gaaaaagcga aaaaagattt atctggcgta tcaagcacac aaatcagctt
    31261 gccatttatc acagcaggag aatctggacc attgcatttg gaaatgacat tgacacgtgc
    31321 aaaatttgat gaattaacag ctgacttagt agaacgtaca aaagtaccag tacgtcaagc
    31381 gctaaaagat gcaggtttat cacaatcaga aatcgacgaa gttattttag tcggtggatc
    31441 tactcgtatc cctgcagttg tagaagcagt acgtaaagaa acaggaaaag aaccaaacaa
    31501 atcagttaac cctgatgaag tagttgctat gggtgctgct atccaaggtg gcgtgatcac
    31561 tggtgatgtg aaagatgttg ttttacttga cgttacacca ttgtcattag gtatcgaaac
    31621 aatgggtgga gtgttcacta aattgattga ccgcaataca acgattccaa caagtaaatc
    31681 acaagtattc tcaactgcgg cagataatca accagcagta gacatccatg tattacaagg
    31741 tgaacgccca atggctgctg acaacaaaac tctaggaaga ttccaattga cagatattcc
    31801 agcagctcct cgtggtattc cacaaatcga agtaacattc gatattgata aaaacgggat
    31861 tgtaaatgta tctgcgaaag atctaggtac acaaaaagaa caaaaaatca cgatcaaatc
    31921 ttcttcaggt ctaacagacg aagaaatcga acgtatggta aaagatgcag aagcaaatgc
    31981 agaagcagat aaagctcgta aagaggaagt tgacctacgt aatgatgtag atgcattgct
    32041 attctctgtg gacaaaacat tgaaagaatt agaaggcaaa gtggacgaag aagaagtgaa
    32101 aaaagcagaa acagctcgcg atgaattgaa agccgctgtt gaagcaaaca acatcgaaga
    32161 aatgaaaact aaacgcgatg cattgaacga aatcgttcaa aacttgactg ttaaactata
    32221 cgaacaagcg gcacaacaac aagctcaaga aaacccagaa gcagctcaag gcggagcaga
    32281 tgatgtcgtt gatgctgatt ttgaagaagt cgatggcgac gataaataat cagttcactg
    32341 aatttcgaga gaaggccaaa gttgctactt tggctttttc ttttgaaaaa tagtttgaag
    32401 tatcggtttt tgtttcaaac aaaatatgat atgataacaa gcgacgtgaa aatgatgaga
    32461 accaatagga ggaaagccta tggcaacaaa acgtgattat tatgaagtct tagggctctc
    32521 aaaaggagca tcagaagatg aaataaagaa agcttatcgt aagctttcga aaaaatacca
    32581 tcctgacatc aataaagaac ctgatgcgga agaaaaattc aaagaagtat cagaagcata
    32641 tgagatttta agtgacccgc aaaaacgcgc tgcatatgac caatacgggc atgcaggcac
    32701 agatccaaat tacggtgctg gtggagctgg tggcggattt ggtggattcg gcggcttctc
    32761 tggtggcggc ttcggtggtt ttgaagatat cttcgactct ttctttggag gaggcggtcg
    32821 gtcagtcgat ccaaatgcgc ctcgtcaagg tgctgatttg caatatacgg tcgacttgtc
    32881 ttttgaagaa gcgatttttg gtgtagaaaa agaaatcaag tataatcggg aagaaatctg
    32941 tcatacttgt ggtgggaacg gtgcaaaacc aggcacacaa ccaactactt gtcataaatg
    33001 tcatggtgca ggtacgatta atgtagaacg ccaaacacca cttggacgag tgatgagccg
    33061 tcaaacttgt gatgtttgtc atgggacagg gaaagaaatc aaagaacctt gtccaacctg
    33121 tcatggctca ggacatgaga agaaagcaca ttcagttaaa gtgaatgtac cagctggtgt
    33181 agaagaagga caacaaatgc gtttagctgg tcaaggagaa gctggagaaa acggcggacc
    33241 atttggtgat ttgtatgtgg tcttccgtgt agaagaaagt gatatctttg atcgtgaagg
    33301 ttctgaaatt tactatgaat tgcctttaag ttttgtacaa gcagctttgg gggatgaagt
    33361 ccaagtacca actgtccatg gagatgtgaa attgaagatc cctgctggaa cacagacagg
    33421 tacgaacttc cgtttacgtg gaaaaggcgc tccgaagtta cgtggtggtg caacaggcga
    33481 ccaacatgtc aaagtaaaac tgatcacacc gaaaaatcta aatgaacaac aaagagaagc
    33541 attacgtgca ttcgctgaag ctggcggcat caaagttgaa gaacagcaag aagacggttt
    33601 cttcgataag tttaaagatg cttttggcgg gaaaaagcgg aagtagtttt tctagtaaaa
    33661 acgctaagtc attaataatt aaaaaccaga cgaacaatca tatgttcgcc tggttttttt
    33721 gtagttgtca tgtttgtacc aacaaaaaag ccagctttag aatatgataa tcgtcatagt
    33781 ttctaggggt ataaccagtg agagaatgga acttgttcaa tcgatattgc aaggtgtttt
    33841 tatgaataaa taattcttct gagcaatttt tgatgcttcc gttattttta ctgaagcatg
    33901 ccaaagtctt cttgaacaaa tgtacttctt cttctgtcag gcttttcaaa atacgtttta
    33961 catataatgt tcgttgatct gacgggattg ctggtaaaac gatcccgagg tctaaatctt
    34021 caaaaaagcg gatctcgttc acagattgat ggaaagctat ccattcgcaa acttgtttgg
    34081 catgaaggaa agattgcggt gtctcttgct ctgtataaca aagcgaccca attccaaagt
    34141 ttagctgtaa ctgatacatt ttattaaact ttgtctggat tttatcaagc aatgatagca
    34201 atgaggctct ggaaatccga ttgactagga taatgatttg ttgtctatgt acagtgagca
    34261 aagcctgtcc gtaataagag aagacccttt cgagcaactc caataaagga ttgatccgtt
    34321 cttgacacaa ttgctgattg tcagaaagtt gtcccacaac tacataatat ggagaagaaa
    34381 aatcaagctc aattaagtga gggtggtatc gactatgtac ttgatatcat caatgaaccg
    34441 atggtacttt cttatgcgat ggaacaggta gagcgattgc ttgtttgtgc aggaaaacaa
    34501 gcgatgcggc tgatacgatt aggtgagaaa ataagaagat aaaaatacac ggtcccttca
    34561 tggcgaccgt gcatttttgg tttttattaa atatttaata ctttattcaa gaaatctttt
    34621 gtccgttcat tttgcggatt ttcaaagatt tgttgaggcg ttccatcttc taaaaagttt
    34681 ccgtcgtcaa tgaacatgac gcgattggcc acttctttag cgaaccccat ttcgtgcgta
    34741 acgataacca tcgtcatacc ttgttctgct aactttttca tgacgttcaa gacatcaccg
    34801 accatttcag gatcgagtgc agaagtaggc tcatcaaaaa gcatgatgtc agggttcatc
    34861 gctagagcac gagcaatagc cacacgctgt ttttgtccac cagaaagcat ttcaggatac
    34921 atatcttttt tatcttctag accaactgtc tgcagtaatt tgattgcttt tttctctgct
    34981 tcttctctag attctttctt caggtccatc ggtgctaaaa tgatgttttc caaaatagaa
    35041 agatgaggga ataggttaaa gtgttggaat accattccga tatgttgtct caccaagttg
    35101 atatctgtgc ttttatccat caagtgggcg ccgtcaataa taatttcacc gtcagaaggt
    35161 tcttctaaac gattcaagca acgcaagaat gtactttttc ctgaacctga agggccaatg
    35221 atacagacga catctccttc gttgatggat acattgatgt cgtttaatac tgtgttatcg
    35281 ccgtattttt tgactaaatg ctcaactaag attttttctg ccatcttact tcaccttctt
    35341 ttctaaaact tttgctaatt ttgtcaatgc tgtgatcaaa atcaagtaca tgatcgcaat
    35401 gatcaagtaa acatacgtac tttgtgttgt acgtgcaaca atgatcttac cagtttgcag
    35461 caattcgact acaccgattg cggaaatgat cgttgtgtct tttaatgaga taacgaactg
    35521 attgacgaat gatgggatca tgatgcgtat cgcttgcggc aaaatgattt tttgcattgt
    35581 ccgattataa gaaagaccta aacttctgga agcttccatt tgtccgacag gaacggcgtt
    35641 gatcccgcct cgaacaatct ctgcgatata agcacttgcg ttaagagtca aggtgataac
    35701 accagcaagg aagtcaggaa tcgtaaatcc aacgatacca ggcaacccaa agaaaataaa
    35761 gaacgcaaga accatcattg gaattcctcg gatgatatcg acataaatac tagcaattgt
    35821 gcgaagagcg cgtattggtg ctacactgaa taatccaaaa atcacaccga ttactatagc
    35881 caaagcaaag gagatcaaag ttaggagaat tgttctccaa agtcctttca acaatgcttg
    35941 atagttgttt tggataagtc caagaaatgt tgattcatca gcagtggaag actcactgga
    36001 agtttctgaa ccatccgcta catattcttt aataatctta tcgtattcgc ctgttctttt
    36061 taattctttc aatccttcat tgaacatatt aagaagctca ggattttgtc cttttttaac
    36121 ggcaaatgca tattttccgc cagattcttt tttgatcggt gtttccaatt cttgtccttg
    36181 tttgatcgca taaccgatta ccggataatc atccatcatt gcatcgattg cgccaacttt
    36241 taacccgtcg tacaattgat cagcagcatc aaaatatttg atagaatagc catattcaga
    36301 ttcatggtcg cttaaaaatt cagcactttc ggtaccaact tttgctccga ctgtttttcc
    36361 tttcaaatct tcataagact tgatcgaagt atctcctttt ttgacagcta attggatccc
    36421 gctatcaaag taagaatcag aaaaatcata gcttttcttt cgttcatctg ttacagtcat
    36481 tccggcaatc attccgtctg cttgtcctga ttccaaagct tgcaaagcag cagagaaacc
    36541 acggtagtcc attcggatgt taaatccttg cagtttggcg atgttttcca tcaattttac
    36601 atcgatgcct tcgtactcgc cctttgcgtt ttgaaattca aatggagcaa aagcagagtc
    36661 actggcaatt acgtatacgt cttttttggg ctctattttt tgcattgtct cgcttgtgct
    36721 tgactctgcc gttgtagtgt tgtcagcttg tgcaacatga ccaaacgcaa gtgtcacaag
    36781 tcctgtcatt aagaaaacaa atgagtgaaa taaatgcttt cttttcataa tcaacctcct
    36841 aaatttccaa tattttatta ttataggcga aaagtgctga atcagacaag actgtaagct
    36901 cgaaaaaatg aaaaaaatgc gagtttctaa cattttatgt tataaaacat aacatgtata
    36961 ataagataaa aaagtggcga aagtatgttc ggtgtggtat aattttttag taaggaatgc
    37021 ctttgctttc cttttctttt tattggtcgt aaaatgaaaa ggaatgaatg aaaaggagga
    37081 cgaagaatga ttgagagaga tacctcccga cactttgagc ttgtctcgaa ataccaaccg
    37141 gctggtgatc agcctgaagc gattaaccaa ttagtggatg gcgtagtcgg cgggaaaaaa
    37201 gcacaaatct tattaggtgc gactggaaca gggaaaacat atacgatttc caatcttatt
    37261 gagaaagtga ataaaccaac actgattatt gcccataaca aaacattggc tggtcagctg
    37321 tacggggaat tcaaggaatt tttccctaac aatgctgttg aatattttgt ttcttattat
    37381 gattattatc agcctgaagc ttatgtgcct tcaagcgata cgtatatcga aaaagattca
    37441 agtgtaaatg atgagatcga caaattacga cattcggcta ctagttcatt attagaaaga
    37501 aatgatgtga tcgtcattgc atccgtttca tgtatttttg gattaggctc gccttttgag
    37561 taccaaaaac aagtagtttc gattcgacag ggagcagaac tagatcgcaa tcagttgatt
    37621 cgtgacttag tcagtatcca attcgaaaga aatgatatag attttcagcg cggtcgtttt
    37681 cgagtgcgcg gtgatgtcgt ggagattttt ccggcttcaa gagatgaacg tgccttacgt
    37741 gtggagtttt ttggagatga ggtcgaacgt atccgagaag tcaatgcact gacaggtgaa
    37801 gttttaggag aaacggaaca cgtagcaatc ttcccggcga ctcactttgt gacgaatgat
    37861 gaacatatgg agcatgccgt agcgaatatc aaagcggaat tagaacagcg tttaactgtc
    37921 ttgcgaaatg aaaacaaact tctggaagct caacgtttag aacagcgaac caattacgat
    37981 attgaaatga tgttagaaat gggctacacg tcgggaattg aaaattattc tcggcatatg
    38041 gacggacgta aagaaggtga accgccttat acgctattgg atttctttcc tgaagatttt
    38101 ttgatcgtgg cggacgaatc gcacgtaacc atgcctcaaa tcagaggaat gtataatggt
    38161 gaccgagcga gaaaacagat gctagtagat tacggtttcc gtttgccatc tgctttagac
    38221 aatcggccac ttcggttaga agagtttgaa aaacatgtca atcagatcat ttatgtctct
    38281 gcgacgccag gaccatatga acatgaacaa acagataccg ttatccaaca aatcatccgt
    38341 ccgacagggc tgcttgaccc agtgatcgaa gttcgtccaa tcatgggaca aatcgatgat
    38401 ctagtaggcg aaataaatga acgagtcgaa aaagaccagc gggtgtttgt aacgacattg
    38461 acgaaaaaaa tggcagaaga cctcacagat tatttcaaag aattagggat caaagtcaaa
    38521 tatttgcata gtgatatcaa aacgctggaa cgaacagaaa tcattcgaga tcttcgatta
    38581 ggtgaatttg atgtgttagt tggaatcaat ttgcttcgtg aaggattaga cgtacctgaa
    38641 gtttcccttg ttgctatctt ggatgcggat aaagaaggct ttttacgtag tgaacgttcc
    38701 ttagtccaaa cgattggacg agctgctcgg aatgaagaag gtaaagtaat catgtacgca
    38761 gacaaagtaa cagattctat gcgtctagcg atggatgaaa cgtcaagacg tcgtacgatt
    38821 cagcagaagt acaatgaaga acatgggatc gttccaaaaa cgatcattaa agaaatccga
    38881 gatttgattt ctattacgaa agaatcggaa gatgatacaa aagaagcggt acaagtctcg
    38941 tacgaagaaa tgacgaagga agaaaaagac acgttattga tgaaattaga aaaagaaatg
    39001 aaagatgcgg caaaagcttt ggattttgaa acagccgcga atgtgcgaga tatgatttta
    39061 gaattaaaag cttcaaagta ggaggacaac atggctaacg ataaaattac gattcatggt
    39121 gcacgtgccc ataacttgaa aaatatagat gtaacgattc cacgagataa attcgtggtc
    39181 gttacaggtt tgtccggttc tggaaaaagt tccctagcat ttgatacgct gtacgctgaa
    39241 gggcagcgga gatacgtaga gagcctttct gcttatgctc gtcagttttt aggacaaatg
    39301 gataagccgg acgtagatag catcgacgga ttgagtccgg cgatttctat tgatcagaaa
    39361 acaacaagta aaaatcctcg gtcgacagta ggtactgtga cggaaatcaa tgactattta
    39421 cgcttgttgt ttgcacgtgt aggacatccg atttgtccca acgatcatat cgagattacc
    39481 agtcaatcag tagatcaaat ggcagatcaa gtaatggctt tgcctgagcg cacaaagatc
    39541 caagtgttgg caccgatcgt ggtaaagaaa aaaggacaac ataaaaaaat attcgaacga
    39601 atccaaaaag agggatacgt ccgcgttcga gtcgatggtg agatctatga tttaagtgaa
    39661 gtgcctgagc tggagaaaaa caaaaaacat gatatcgcga tcgtcatcga ccggatcatt
    39721 gtgaaagaag ggattcgttc tcgcctattt gattctttag aggcggccct gcgattagca
    39781 gacggctatg ctctggttga cgtgatcggt gaagaagaaa tgttgtttag cgaacactat
    39841 gcctgtcctt attgcggatt tactgttggc gaactagaac caagactgtt ctcattcaat
    39901 gcgccttttg gagcttgtcc agactgtgat ggtttaggtg tcaaattaga agtcgatcaa
    39961 gatttagtga ttccagatag aacgaagaca cttagtcaag gtgctatcgt tccatggaat
    40021 ccaatcagct ctcagtatta tcctcaaatg ttagcgcagg cttgtgaaag tttcggtatc
    40081 gatttagata caccattcga agaattaccg gaagagcatc aggagatcat cttgaatggg
    40141 acagatgatc ttttccattt ccattatgaa aatgattttg gtggcgttcg cgatgtagaa
    40201 acgactttcg aaggggtatt gaagaatatc gagcgtcgtt atcatgatac gaacagtgat
    40261 ttcacgcgtg agcaaatgcg tctttatatg acagaattaa cttgtcagac atgtaaaggt
    40321 tatcgattga atccccaagc tctatctgtt caaatcaatg gaacgaatat cgggcaaaca
    40381 aatgagttgt ctattcaaaa agcagtagat ttcttctcaa acttaacgtt atctgaacaa
    40441 gagaaagtaa ttgccaaacc gattttaaaa gaagtaaatg acagactttc attcttggaa
    40501 aatgtgggac tagactatct aactttgagt cgcgcttccg gaacattatc tgggggagaa
    40561 gcgcagcgaa tcagattggc tacacaaatt ggatcgaatc tttctggagt tttgtatatt
    40621 ttagatgaac catcgatcgg attgcatcaa cgcgacaacg atcgactgat tggctcatta
    40681 aagaaaatgc gtgatcttgg caacaccctc gttgtagtcg aacatgatga agatacgatg
    40741 tgggcagcag attatttgat tgacgtcgga cctggggccg gagaacaagg cggacaaatc
    40801 gtggctgcag gcactcctga agaagtagct aatgatgaaa attctttgac agggaaatat
    40861 ctttcaggga aaaaatcaat ccctgtacca aaagaacgcc gtaaaggtaa tgggaaagca
    40921 atcaagatca ctggtgcttc agaaaacaat ctgaaaaata ttagtgttga atttccatta
    40981 ggagaatttg tagcagtcac aggtgtgtct ggttctggaa agagtacatt ggtcaatagt
    41041 atcttaaaga aatcgttagc gcaaaaatta aataagaatt ctgctaagcc aggtaaattc
    41101 aagacaattt ccggctacga aagtatcgaa aagatcatcg atatcgatca aagcccaatc
    41161 ggccggacgc cgagaagtaa tccagcgact tatacaagtg tatttgatga tatccgtggg
    41221 ttatttgctc aaacgaacga ggcaaaaatg cggggttata agaaagggcg ttttagtttc
    41281 aacgtaaaag gcggtcgttg tgaagcttgt cgcggggatg gaattattaa gatcgaaatg
    41341 cactttttgc ctgatgtcta tgttccttgt gaagtatgtc atggtaaacg atataattct
    41401 gaaacattag aagtgcatta caaaggaaaa agcattgctg atattttgga aatgacagta
    41461 gaggatgctg tagaattctt caagcacatt ccaaagattc atcgcaaact gcaaacgatt
    41521 gttgatgttg gcttaggtta tgtgacgatg gggcaaccag caacgacatt gtccggtggt
    41581 gaggcacaac ggatgaaact tgccagtgaa ttgcacaaaa tctctaatgg aaagaatttc
    41641 tatatactag atgaaccaac gacaggactt catagcgatg acatcgcccg cttgttgcat
    41701 gtattacaaa gattagtaga tgctggtaac acagttttag tgattgaaca caatctagat
    41761 gtaatcaaaa cagcagatta tatcattgat ttaggaccag aaggtggaga aggtggagga
    41821 acgatccttg cgactggaac accagaagaa atcattaacg taaaagaaag ttatacaggt
    41881 cactatttga aaaaaataat ggtataaaga ggatagatag ctgagatagt taaaaaaaca
    41941 taaaaaaatg aatttttgct tgttttatta atatcagtgt tctagaatca ttacatggta
    42001 gttctttcta cactgattgg tatagataaa aacttcctat aagttcaatc cgttcaaaaa
    42061 agaattgagc aatcggagga ctttcctttg attgcttttt tctttatggt atgatattcc
    42121 ttgtgtagtt ggaaaagaaa aacaagagat tatgagtaag tgagagataa tgatgaacag
    42181 tattgaaaat gaagaattta tccctatatt attaggaagc gacatgaacg tttatggtat
    42241 ggcacgttct tttaatgaag catacggcaa aatctgccaa gcatatgctt cggatcagtt
    42301 agcccctacc cgctacagta aaattgtcaa tgtagaagtt attccaggat ttgataaaga
    42361 ccctgtattt atcgagacga tgcttcgttt ggcaaaagaa agatattctg ataagagtaa
    42421 aaaatatctt ttgattgctt gtggtgatgg atatgcagaa ttgatctccc aacataaaca
    42481 agagctgtca gaatatttta tctgtccata tattgattat tctttatttg aaaggctgat
    42541 caataaagta agcttttatg aagtttgtga agaatatgat cttccgtatc ctaaaacatt
    42601 gatcgtcaga gaagaaatgt tagtaaatgg acatttggaa caggaattgc cttttgaatt
    42661 cccagttgct ttgaaacctg caaacagtgt ggagtatctt tctgtccaat ttgaaggaag
    42721 aaaaaaagct tttattttag agactagaga agagttcgat ttaattttag gccggatata
    42781 cgaagccggt tataaaagtg aaatgattgt acaagatttt atcccgggtg atgatagtaa
    42841 tatgcgtgtg ctaaatgcgt atgtagatga agatcatcaa gttcggatga tgtgccttgg
    42901 tcatccacta ttagaagatc ctacaccagc atcgattggt aattacgttg tgatcatgcc
    42961 agattataac gaaaaaatct atcaaaccat taaggcattt ttggaaaaaa tcgaatatac
    43021 aggctttgcc aatttcgata tgaagtatga tcctagagat ggagaatata aattatttga
    43081 aatcaatttg agacaaggtc gtagcagttt ctttgtgaca ttgaacggct tgaatctggc
    43141 tcgttttgtc acagaagatc gagtgttcaa caaacccttt gttgaaacga cctatggaac
    43201 gaatcaatca gataaagcaa gattatggat gggtgtgccc aaaaaaatct ttttagaata
    43261 tgctcgggaa aatgaagaca aaaaactagc tgagcaaatg atcaaagaaa atcgttacgg
    43321 tactactgta ttttatgaaa aagaccgatc aatcaagcgc tggttgttga tgaaatatat
    43381 gttccataac tatattccaa gattcaaaaa gtattttcat gtgaaagaag gctagttcat
    43441 ggagaatttt ttcagtattt taggcggaat gggcacgatg gcgacagaaa gttttgttcg
    43501 cttgatcaac caccgaacga aagctacaaa agatcaagaa tatttgaatt atgtcttatt
    43561 caatcatgca acagttcctg atcgtacagc ttatatttta gatcgatcag aagaaaatcc
    43621 aatgccgttt ctgctggatg atattgagaa acaaaattta ttgcgaccaa acttcattgt
    43681 gttgacttgt aacaccgcac actacttttt tgaagagtta caagcggcaa cggatattcc
    43741 tatcttgcat atgccaagag aagcagcaaa tgaacttgtc cgtcagcata cgacagggag
    43801 agtagcgatt ctaggaacag aaggaagtat gaaagctgga atctatgaac gcgaagtcaa
    43861 aaatcttggt ttcgaaacag tgattcctga tactgcgctt caagaaaaaa tcaattacct
    43921 gatctatcat gagatcaagg aatcggatta tttgaatcaa gagctctatt acgaaatctt
    43981 agaggaagcg gtagagcgcc taaactgtga aaaagtcatc ctaggttgca cagaattatc
    44041 tttgatgcat gaatttgctg aagataatca ttacccagtg atcgatgctc aatctatttt
    44101 agcggatcga acgatcgaac gtgcattagc tgaaagaagc gaggcattgg atacagcatc
    44161 ggaaaagtaa aaaaattgga tttggggcaa atatcctaaa agatatttgg tccaaatctt
    44221 tttgtaaaag aaaaagaaaa ctggtaatga tacgcccggg atgatataat tttattatgc
    44281 acaatgtgag aggggttcgt aaatatgaca gataatttgc aattagtcat cataacaggt
    44341 atgagtggtg cagggaaaac agtagctatc caaagttttg aagatatggg ctatttttgt
    44401 atcgataata tgccgcctcg attgattcca aaattctggg aattgatcaa agaatcaggt
    44461 aaagtcacaa aaatggcttt aggagtggat ttgcgttctc gttccttctt tgaagaaatc
    44521 caaaacatgc tgatcgaaat cgaaaatacg aacttcatcg acacttctat cttattttta
    44581 gatgcttcag acgaagaact agtggctcgc tacaaagaaa caagacgagc acatccaatg
    44641 gcgatggacg gtttgatcac tgaagggata cgtaaagaac gggcgatact agacgacttg
    44701 aaagcccaag cttccgtgat cattgatacg acaacattga caccacgaca attacgtgaa
    44761 aaaatcaatg agtcattcaa atcaaaggga gaccaaggat ttcggatcga atttgtatca
    44821 tttggtttta aatacgggtt gccgatcgat tcggatattg tgatggatgt gcgttttttg
    44881 cccaaccctc attatatccc ggaactaaga ccattgaccg gaaaagatca gccagtctat
    44941 gattatgtga tgtcctttga tgaaacgcaa tcattctacc ataaattttt ggatcttctg
    45001 gaaactgtat tgcctggata cattaaagaa ggaaaaagca gcttgacagt tgcaatcggc
    45061 tgtacaggcg gtcaacaccg ttctgtggca ttgactgaac gtttggcaaa tgctttaggg
    45121 aaaaactata aagtcaatgt gacacatcgg gacaaggata aaaggaaaga gacggtgaac
    45181 cggtcgtgaa aatgaaaacc taccggataa gaaaaccaaa gatcgtagtg gtcggcggtg
    45241 gcactggtct gccagttatc ctgaaaagtt tacgaaatca aggagcagat atcacagcag
    45301 tagtgactgt tgcagatgat ggtggaagca gcggtgcaat cagagaatcg atcgctatgg
    45361 ctcctcctgg tgacttgaga aatgtcttag tggcgttatc ggatatgcca caattctatg
    45421 aagatatttt ccaataccgt ttcaaaaagg aagatcagtt tttagccaat catacgatcg
    45481 gtaatctgat cattgccgca gtttccgaga tgcggggaag cacttatgag gcgattcaat
    45541 tattagcaaa aatgatgcat gtagatggac atgtttatcc ttcttcagaa actcctttga
    45601 ccttgcatgc tgtttttaaa gacggaacga ctgcagtagg agaatctaaa atcgcgatca
    45661 accgtaaaac cattgaccgt gtgtatgtct gcaacacaaa tgataaaagt gaagccaaag
    45721 cggctcgaaa agtcgtttca gcaattatgg acgccgacat ggtcgttttg ggacccggaa
    45781 gtttattcac cagtatcctt ccaaatttag tgataccaga aatcggcgaa gcaatgctca
    45841 agacaactgc tgaaaccgtc tatatctgta atatcatgac ccaaaaaggg gaaacggaac
    45901 atttcacaga tgccgatcat atacgtgttt tacacaagca tttgaatcag ccttttatcg
    45961 atacagtatt ggtcaatacg gaaaaagtgc ctgaagatta tatggaccca gagatctatg
    46021 atgagtatct agtacaagtc gcacacgact tcaacggatt gagaaatgaa ggatgtcgag
    46081 tgatttccac cgacttttta gaattgaaag atggtggtgt cttccacgat ggagacaaag
    46141 tagtagaaga attgttccgt ttagttttcg gttcaaaata ttaagcaatt tattgttgga
    46201 gaaaggagga gaataggatg tcatttgctg cagatgtgaa gaaagaacta actggtttgg
    46261 aagttcatcg agaacatgcg aaagcggaat tagctgcttt gctgcgaatg aacggctctt
    46321 taggtattgt gaaccagcag tttattttga acgtccaaac agaaaatgct gcaattgcca
    46381 gacgtatcta ttctcttttg aaagatcatt atcaggtacg aagtgagcta ctcgttcgta
    46441 aaaaaatgaa attaaaaaag aacaatgtct atatcgtccg gttgaagcaa ggcacaaaag
    46501 atgtgttgat ggatcttgat atcatggatg gtatgatgtt caatggacac gtttcggacg
    46561 agattatggg aaattcgcaa aaaatgcgtt cttacttacg tggtgctttt atggcctcag
    46621 gttcagtcaa taatccggaa acgagtcgtt atcatttgga aatattttct atttatgaac
    46681 agcataacca agacatttgc gatatgttaa attattacgg tctacatgct cgaacgcttg
    46741 aacggcgcaa tggctatatt tcgtatttaa aaggagcaga aaaaattgct gatttcttaa
    46801 cattgattgg tgcaaccaac tcaatgctcc gatttgaaga tgttcgaatc gtacgggata
    46861 tgagaaattc ggtcaatcgt ctagtcaatt gtgagacagc aaatctgaac aagacgattg
    46921 atgcggcttc taaacaaatc gaaaacatcg aattgatcga agcaaaggtc ggtctacatg
    46981 cccttcctga aaaacttcag gaaatcgcag aacttcggct tcaacatcca gaggtcagct
    47041 tgaaagaatt aggggaaatg attccctcag gtgcaatatc aaaatcggga attaatcacc
    47101 gaattcgcaa aataaatgaa tttgcggaaa aactgcgtga agaagcagtt taatccaaag
    47161 aaaaaactga tacatgaagc aggagatgcg aatgtatcag ttttttttaa aagctatact
    47221 tgcttttctt ccgtcaaact ataattccaa ctttactgga gtaacataat gttgaaaggc
    47281 ttctattgtt ttttaataca atcgtataaa aagcggggag acaactgaat gaagatttta
    47341 gttttataca taaacttttg cttaatatag aagtcctgat atagatagga tcaagtattt
    47401 atctataact ggagattaat taaaaactgg aaggaactgc tcgatcaacc ttgagaactg
    47461 tttttcttac ttctggtcaa gattctcgtg gttggtggat aaggatggtt gataagttca
    47521 ttggattagc tagtcgaaaa caaccaggtg taattaatgt agaagtttta acaaggggat
    47581 ggagtgattt attcagtgat aatcataagt tcataaatag taattgggat gtatggttgg
    47641 tatacgttat acctgaactt gaactatcaa gatttggttt agaatttgag aatcgatgtt
    47701 tataaaggga gggcaaaaaa tgtgcttttt tacaactttt actatcatta tgattgcagt
    47761 actttttatt tttctagata tagcaaagcg aaatacagca tttttattat atcgggtttt
    47821 acttcgagca ggtctcataa catttatatc gatcgttgga ttttttctat tcacagttat
    47881 tgtatttatt tggagaacta cagcaccacc attgccagag attacatatg gagaatttcc
    47941 gtttagtctt gaatatgaaa tagatgagga aagaattttg attgaagata cggtgattgc
    48001 agaatttata ggaagtttta gagggaatgt gacaactcaa cctagacgaa gttggagtat
    48061 aaaactttta aataatagtg gagagttgaa agcatttcat tatgaattct taataataga
    48121 aagtgaagat gtaacaatca ctttaagtag tggctctgct ggatatttta tgaatgatgg
    48181 tggaagcaag ccacagctca cagtccgtga tcgtattgca ggttttatgc atttttctca
    48241 ctatgactta gaagaagctc gtaaaatact tgcagaatat gggatcacat taatcaattg
    48301 ggaatatgcg ccaccgataa gaaatttttt caatgattat tattagtctt ataaaaaaca
    48361 gaatgtaaaa tttatgtgcg cgagtattta gttttccatt ttttgtttga tagatatata
    48421 gcattgttga agcatgggat agtggtgcca attttatcaa tgaggttggt caggcagtta
    48481 acataattgg tgatgctata aatccattta attggaattg gaggaattag gtgtggaaac
    48541 agtgagatta tggatattca tgaaaaattt tcgttcggct ttgagatatg ttattctgga
    48601 atcaaaatca ggttattatc tgattgacac agatcactat actttttggg gaggctacct
    48661 ttttccgatt tttaattgga taatccccca taaggttatg aaaattaact tgcctgcaga
    48721 agaaataaat agattaatac tcaacaaaga tttacaaagg aaaagagaga aagaacattc
    48781 taatgttgct tggataggtc cagcatttgc agtagtgact tcttcaattg tcggtcctat
    48841 gctagagagt atttatagtg atttttctat tggaatacgt tatttatttg ttactttaag
    48901 tatagctgtt cttttgtgca taaagggtta tgttagcaag aagaataaag atattttaac
    48961 actaatcggt gtaaacaatt tatctagtac taaaattata ctgtttcctg aatctataaa
    49021 agaaggatca aggttgttga taatttttat aatgcttata ctgttttctg ttttatttgg
    49081 ggtgatgttt ataga
//
LOCUS       DAYYAO010000011        44911 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000011 DAYYAO010000000
VERSION     DAYYAO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.012"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1008)
                     /locus_tag="ACHARI_000554"
     CDS             complement(<1..1008)
                     /locus_tag="ACHARI_000554"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8441629.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1188..1532)
                     /locus_tag="ACHARI_000555"
     CDS             complement(1188..1532)
                     /locus_tag="ACHARI_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8441630.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1525..1959)
                     /locus_tag="ACHARI_000556"
     CDS             complement(1525..1959)
                     /locus_tag="ACHARI_000556"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8441631.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2097..2834
                     /locus_tag="ACHARI_000557"
     CDS             2097..2834
                     /locus_tag="ACHARI_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441632.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2827..4038
                     /locus_tag="ACHARI_000558"
     CDS             2827..4038
                     /locus_tag="ACHARI_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441633.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4242..5024
                     /locus_tag="ACHARI_000559"
     CDS             4242..5024
                     /locus_tag="ACHARI_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8441634.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5094..5756
                     /gene="trmB"
                     /locus_tag="ACHARI_000560"
     CDS             5094..5756
                     /gene="trmB"
                     /locus_tag="ACHARI_000560"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8441635.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5854..7608)
                     /gene="ade"
                     /locus_tag="ACHARI_000561"
     CDS             complement(5854..7608)
                     /gene="ade"
                     /locus_tag="ACHARI_000561"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8441636.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7740..8669
                     /gene="comGA"
                     /locus_tag="ACHARI_000562"
     CDS             7740..8669
                     /gene="comGA"
                     /locus_tag="ACHARI_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8441637.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8626..9675
                     /gene="comGB"
                     /locus_tag="ACHARI_000563"
     CDS             8626..9675
                     /gene="comGB"
                     /locus_tag="ACHARI_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8441638.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9672..9950
                     /gene="comGC"
                     /locus_tag="ACHARI_000564"
     CDS             9672..9950
                     /gene="comGC"
                     /locus_tag="ACHARI_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8441639.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9925..10395
                     /gene="comGD"
                     /locus_tag="ACHARI_000565"
     CDS             9925..10395
                     /gene="comGD"
                     /locus_tag="ACHARI_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8441640.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10367..10714
                     /locus_tag="ACHARI_000566"
     CDS             10367..10714
                     /locus_tag="ACHARI_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441641.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10677..11102
                     /gene="comGF"
                     /locus_tag="ACHARI_000567"
     CDS             10677..11102
                     /gene="comGF"
                     /locus_tag="ACHARI_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8441642.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11074..11433
                     /gene="comGG"
                     /locus_tag="ACHARI_000568"
     CDS             11074..11433
                     /gene="comGG"
                     /locus_tag="ACHARI_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8441643.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11605..12612
                     /locus_tag="ACHARI_000569"
     CDS             11605..12612
                     /locus_tag="ACHARI_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8441644.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12637..13821
                     /locus_tag="ACHARI_000570"
     CDS             12637..13821
                     /locus_tag="ACHARI_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8441645.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="ACHARI_000571"
     CDS             complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="ACHARI_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8441646.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15946..16074)
                     /locus_tag="ACHARI_000572"
     CDS             complement(15946..16074)
                     /locus_tag="ACHARI_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441647.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16419..16562)
                     /locus_tag="ACHARI_000573"
     CDS             complement(16419..16562)
                     /locus_tag="ACHARI_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8441648.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16785..17264)
                     /locus_tag="ACHARI_000574"
     CDS             complement(16785..17264)
                     /locus_tag="ACHARI_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8441649.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17337..17621)
                     /locus_tag="ACHARI_000575"
     CDS             complement(17337..17621)
                     /locus_tag="ACHARI_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441650.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17699..17893)
                     /gene="ssrS"
                     /locus_tag="ACHARI_000576"
     ncRNA           complement(17699..17893)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHARI_000576"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17965..19251)
                     /locus_tag="ACHARI_000577"
     CDS             complement(17965..19251)
                     /locus_tag="ACHARI_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8441651.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19389..20060
                     /locus_tag="ACHARI_000578"
     CDS             19389..20060
                     /locus_tag="ACHARI_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8441652.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20026..20514
                     /locus_tag="ACHARI_000579"
     CDS             20026..20514
                     /locus_tag="ACHARI_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8441653.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20527..21474
                     /gene="prmA"
                     /locus_tag="ACHARI_000580"
     CDS             20527..21474
                     /gene="prmA"
                     /locus_tag="ACHARI_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8441654.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21475..22239
                     /locus_tag="ACHARI_000581"
     CDS             21475..22239
                     /locus_tag="ACHARI_000581"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8441655.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22472..24685
                     /locus_tag="ACHARI_000582"
     CDS             22472..24685
                     /locus_tag="ACHARI_000582"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8441656.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24696..25142
                     /gene="dtd"
                     /locus_tag="ACHARI_000583"
     CDS             24696..25142
                     /gene="dtd"
                     /locus_tag="ACHARI_000583"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8441657.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25313..26572
                     /locus_tag="ACHARI_000584"
     CDS             25313..26572
                     /locus_tag="ACHARI_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8441658.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26661..27608)
                     /locus_tag="ACHARI_000585"
     CDS             complement(26661..27608)
                     /locus_tag="ACHARI_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8441659.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28002..29294
                     /gene="hisS"
                     /locus_tag="ACHARI_000586"
     CDS             28002..29294
                     /gene="hisS"
                     /locus_tag="ACHARI_000586"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8441660.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29309..31078
                     /gene="aspS"
                     /locus_tag="ACHARI_000587"
     CDS             29309..31078
                     /gene="aspS"
                     /locus_tag="ACHARI_000587"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8441661.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31187..32062
                     /locus_tag="ACHARI_000588"
     CDS             31187..32062
                     /locus_tag="ACHARI_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8441662.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32328..34211
                     /locus_tag="ACHARI_000589"
     CDS             32328..34211
                     /locus_tag="ACHARI_000589"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8441663.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34286..34570)
                     /locus_tag="ACHARI_000590"
     CDS             complement(34286..34570)
                     /locus_tag="ACHARI_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8441664.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34560..35294)
                     /locus_tag="ACHARI_000591"
     CDS             complement(34560..35294)
                     /locus_tag="ACHARI_000591"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8441665.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35423..36046
                     /locus_tag="ACHARI_000592"
     CDS             35423..36046
                     /locus_tag="ACHARI_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8441666.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36238..37359
                     /locus_tag="ACHARI_000593"
     CDS             36238..37359
                     /locus_tag="ACHARI_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8441667.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37366..40494
                     /locus_tag="ACHARI_000594"
     CDS             37366..40494
                     /locus_tag="ACHARI_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8441668.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40513..43707
                     /locus_tag="ACHARI_000595"
     CDS             40513..43707
                     /locus_tag="ACHARI_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8441669.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43853..44560)
                     /locus_tag="ACHARI_000596"
     CDS             complement(43853..44560)
                     /locus_tag="ACHARI_000596"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8441670.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14414 a         7969 c         9412 g        13116 t
ORIGIN      
        1 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
       61 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
      121 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
      181 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
      241 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
      301 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
      361 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
      421 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
      481 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
      541 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
      601 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
      661 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
      721 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
      781 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
      841 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
      901 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
      961 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     1021 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     1081 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     1141 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     1201 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     1261 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     1321 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     1381 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     1441 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     1501 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     1561 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     1621 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     1681 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     1741 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     1801 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     1861 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     1921 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     1981 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     2041 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     2101 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     2161 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     2221 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     2281 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     2341 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     2401 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     2461 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     2521 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     2581 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     2641 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     2701 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     2761 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     2821 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     2881 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     2941 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     3001 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     3061 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     3121 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     3181 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     3241 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     3301 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     3361 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     3421 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     3481 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     3541 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     3601 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     3661 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     3721 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     3781 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     3841 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     3901 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     3961 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     4021 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     4081 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     4141 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     4201 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     4261 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     4321 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     4381 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     4441 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     4501 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     4561 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     4621 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     4681 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     4741 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     4801 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     4861 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     4921 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     4981 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     5041 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     5101 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     5161 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     5221 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     5281 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     5341 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     5401 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     5461 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     5521 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     5581 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     5641 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     5701 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     5761 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     5821 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     5881 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     5941 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     6001 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     6061 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     6121 attgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     6181 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     6241 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     6301 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     6361 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     6421 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     6481 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     6541 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     6601 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     6661 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     6721 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     6781 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     6841 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     6901 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     6961 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     7021 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     7081 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     7141 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     7201 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     7261 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     7321 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     7381 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     7441 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     7501 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     7561 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     7621 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     7681 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     7741 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     7801 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     7861 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     7921 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     7981 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     8041 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     8101 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     8161 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     8221 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     8281 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     8341 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     8401 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     8461 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     8521 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     8581 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     8641 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     8701 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     8761 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     8821 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     8881 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     8941 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     9001 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     9061 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     9121 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     9181 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     9241 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     9301 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     9361 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     9421 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     9481 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     9541 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     9601 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     9661 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     9721 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     9781 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     9841 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     9901 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     9961 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    10021 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    10081 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    10141 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    10201 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    10261 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    10321 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    10381 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    10441 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    10501 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    10561 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    10621 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    10681 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    10741 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    10801 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    10861 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    10921 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    10981 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    11041 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    11101 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    11161 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    11221 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    11281 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    11341 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    11401 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    11461 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    11521 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    11581 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    11641 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    11701 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    11761 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    11821 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    11881 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    11941 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    12001 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    12061 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    12121 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    12181 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    12241 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    12301 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    12361 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    12421 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    12481 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    12541 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    12601 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    12661 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    12721 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    12781 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    12841 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    12901 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    12961 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    13021 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    13081 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    13141 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    13201 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    13261 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    13321 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    13381 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    13441 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    13501 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    13561 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    13621 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    13681 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    13741 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    13801 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    13861 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    13921 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    13981 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    14041 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    14101 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    14161 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    14221 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    14281 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    14341 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    14401 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    14461 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    14521 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    14581 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    14641 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    14701 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    14761 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    14821 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    14881 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    14941 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    15001 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    15061 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    15121 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    15181 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    15241 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    15301 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    15361 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    15421 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    15481 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    15541 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    15601 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    15661 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    15721 tgccagttcc gttgataccg actaagacaa ttttttcacc agcttcaatt tgacaggata
    15781 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    15841 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    15901 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    15961 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    16021 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    16081 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    16141 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    16201 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    16261 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    16321 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    16381 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    16441 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    16501 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    16561 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    16621 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    16681 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    16741 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    16801 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    16861 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    16921 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    16981 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    17041 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    17101 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    17161 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    17221 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    17281 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    17341 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    17401 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    17461 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    17521 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    17581 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    17641 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    17701 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    17761 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    17821 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    17881 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    17941 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    18001 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    18061 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    18121 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    18181 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    18241 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    18301 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    18361 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    18421 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    18481 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    18541 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    18601 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    18661 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    18721 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    18781 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    18841 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    18901 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    18961 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    19021 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    19081 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    19141 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    19201 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    19261 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    19321 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    19381 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    19441 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    19501 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    19561 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    19621 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    19681 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    19741 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    19801 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    19861 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    19921 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    19981 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    20041 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    20101 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    20161 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    20221 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    20281 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    20341 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    20401 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    20461 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    20521 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    20581 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    20641 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    20701 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    20761 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    20821 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    20881 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    20941 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    21001 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    21061 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    21121 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    21181 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    21241 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    21301 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    21361 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    21421 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    21481 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    21541 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    21601 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    21661 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    21721 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    21781 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    21841 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    21901 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    21961 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    22021 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    22081 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    22141 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    22201 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    22261 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    22321 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    22381 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    22441 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    22501 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    22561 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    22621 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    22681 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    22741 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    22801 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    22861 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    22921 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    22981 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    23041 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    23101 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    23161 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    23221 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    23281 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    23341 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    23401 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    23461 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    23521 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    23581 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    23641 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    23701 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    23761 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    23821 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    23881 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    23941 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    24001 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    24061 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    24121 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    24181 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    24241 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    24301 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    24361 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    24421 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    24481 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    24541 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    24601 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    24661 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    24721 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    24781 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    24841 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    24901 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    24961 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    25021 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    25081 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    25141 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    25201 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    25261 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    25321 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    25381 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    25441 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    25501 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    25561 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    25621 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    25681 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    25741 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    25801 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    25861 aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    25921 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    25981 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    26041 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    26101 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    26161 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    26221 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    26281 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    26341 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    26401 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    26461 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    26521 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    26581 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    26641 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    26701 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    26761 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    26821 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    26881 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    26941 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    27001 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    27061 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    27121 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    27181 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    27241 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    27301 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    27361 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    27421 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    27481 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    27541 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    27601 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    27661 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    27721 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    27781 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    27841 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    27901 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    27961 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    28021 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    28081 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    28141 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    28201 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    28261 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    28321 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    28381 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    28441 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    28501 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    28561 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    28621 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    28681 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    28741 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    28801 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    28861 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    28921 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    28981 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    29041 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    29101 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    29161 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    29221 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    29281 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    29341 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    29401 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    29461 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    29521 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    29581 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    29641 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    29701 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    29761 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    29821 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    29881 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    29941 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    30001 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    30061 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    30121 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    30181 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    30241 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    30301 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    30361 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    30421 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    30481 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    30541 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    30601 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    30661 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    30721 tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt ctatccgtat
    30781 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    30841 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    30901 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    30961 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    31021 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    31081 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    31141 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    31201 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    31261 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    31321 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    31381 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    31441 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    31501 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    31561 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    31621 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    31681 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    31741 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    31801 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    31861 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    31921 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    31981 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    32041 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    32101 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    32161 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    32221 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    32281 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    32341 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    32401 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    32461 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    32521 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    32581 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    32641 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    32701 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    32761 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    32821 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    32881 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    32941 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    33001 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    33061 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    33121 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    33181 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    33241 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    33301 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    33361 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    33421 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    33481 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    33541 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    33601 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    33661 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    33721 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    33781 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    33841 taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg aatggctcat
    33901 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    33961 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    34021 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    34081 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    34141 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    34201 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    34261 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    34321 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    34381 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    34441 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    34501 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    34561 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    34621 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    34681 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    34741 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    34801 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    34861 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    34921 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    34981 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    35041 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    35101 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    35161 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    35221 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    35281 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    35341 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    35401 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    35461 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    35521 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    35581 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    35641 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    35701 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    35761 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    35821 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    35881 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    35941 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    36001 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    36061 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    36121 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    36181 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    36241 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    36301 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    36361 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    36421 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    36481 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    36541 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    36601 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    36661 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    36721 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    36781 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    36841 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    36901 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    36961 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    37021 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    37081 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    37141 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    37201 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    37261 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    37321 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    37381 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    37441 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    37501 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    37561 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    37621 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    37681 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    37741 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    37801 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    37861 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    37921 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    37981 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    38041 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    38101 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    38161 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    38221 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    38281 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    38341 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    38401 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    38461 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    38521 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    38581 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    38641 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    38701 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    38761 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    38821 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    38881 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    38941 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    39001 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    39061 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    39121 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    39181 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    39241 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    39301 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    39361 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    39421 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    39481 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    39541 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    39601 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    39661 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    39721 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    39781 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    39841 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    39901 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    39961 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    40021 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    40081 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    40141 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    40201 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    40261 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    40321 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    40381 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    40441 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    40501 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    40561 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    40621 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    40681 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    40741 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    40801 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    40861 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    40921 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    40981 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    41041 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    41101 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    41161 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    41221 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    41281 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    41341 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    41401 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    41461 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    41521 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    41581 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    41641 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggc
    41701 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    41761 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    41821 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    41881 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    41941 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    42001 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    42061 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    42121 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    42181 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    42241 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    42301 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    42361 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    42421 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    42481 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    42541 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    42601 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    42661 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    42721 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    42781 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    42841 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    42901 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    42961 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    43021 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    43081 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    43141 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    43201 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    43261 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    43321 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    43381 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    43441 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    43501 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    43561 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    43621 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    43681 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    43741 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    43801 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    43861 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    43921 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    43981 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    44041 tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    44101 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    44161 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    44221 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    44281 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    44341 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    44401 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    44461 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    44521 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    44581 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    44641 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    44701 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga acaaattaat
    44761 cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt ttcgaaagat
    44821 ttattttttt aatacagtct caatataact attcaaacat aaaataacgt tatttgattt
    44881 atttgtttat agtaaatttt atttagtaat c
//
LOCUS       DAYYAO010000012        44883 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000012 DAYYAO010000000
VERSION     DAYYAO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.013"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHARI_000597"
     CDS             212..604
                     /locus_tag="ACHARI_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8441671.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHARI_000598"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHARI_000598"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8441672.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHARI_000599"
     CDS             2698..2943
                     /locus_tag="ACHARI_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441673.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHARI_000600"
     CDS             3165..4775
                     /locus_tag="ACHARI_000600"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8441674.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHARI_000601"
     CDS             complement(4752..4964)
                     /locus_tag="ACHARI_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441675.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHARI_000602"
     CDS             5067..5936
                     /locus_tag="ACHARI_000602"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8441676.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHARI_000603"
     CDS             6101..7372
                     /locus_tag="ACHARI_000603"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8441677.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHARI_000604"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHARI_000604"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8441678.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHARI_000605"
     CDS             8763..9026
                     /locus_tag="ACHARI_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8441679.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHARI_000606"
     CDS             9471..9920
                     /locus_tag="ACHARI_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8441680.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHARI_000607"
     CDS             9977..11395
                     /locus_tag="ACHARI_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8441681.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHARI_000608"
     CDS             11395..12255
                     /locus_tag="ACHARI_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8441682.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHARI_000609"
     CDS             12429..13202
                     /locus_tag="ACHARI_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8441683.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHARI_000610"
     CDS             13349..13714
                     /locus_tag="ACHARI_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8441684.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHARI_000611"
     CDS             complement(13862..15070)
                     /locus_tag="ACHARI_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8441685.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHARI_000612"
     CDS             complement(15089..15367)
                     /locus_tag="ACHARI_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441686.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHARI_000613"
     CDS             15560..16216
                     /locus_tag="ACHARI_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8441687.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHARI_000614"
     CDS             complement(16658..17455)
                     /locus_tag="ACHARI_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8441688.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHARI_000615"
     CDS             complement(17452..18636)
                     /locus_tag="ACHARI_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8441689.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHARI_000616"
     CDS             complement(18661..19908)
                     /locus_tag="ACHARI_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8441690.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHARI_000617"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHARI_000617"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8441691.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHARI_000618"
     CDS             21888..22202
                     /locus_tag="ACHARI_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8441692.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHARI_000619"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHARI_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8441693.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHARI_000620"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHARI_000620"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8441694.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHARI_000621"
     CDS             23546..23875
                     /locus_tag="ACHARI_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8441695.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHARI_000622"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHARI_000622"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8441696.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHARI_000623"
     CDS             24863..25687
                     /locus_tag="ACHARI_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8441697.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHARI_000624"
     CDS             25680..26027
                     /locus_tag="ACHARI_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8441698.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHARI_000625"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHARI_000625"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8441699.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHARI_000626"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHARI_000626"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8441700.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHARI_000627"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHARI_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8441701.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHARI_000628"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHARI_000628"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8441702.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHARI_000629"
     CDS             31684..32451
                     /locus_tag="ACHARI_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441703.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFIVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHARI_000630"
     CDS             32441..34483
                     /locus_tag="ACHARI_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441704.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACHARI_000631"
     CDS             34764..34913
                     /locus_tag="ACHARI_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8441705.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACHARI_000632"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACHARI_000632"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8441706.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACHARI_000633"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACHARI_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8441707.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACHARI_000634"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACHARI_000634"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8441708.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACHARI_000635"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACHARI_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8441709.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACHARI_000636"
     CDS             39330..40412
                     /locus_tag="ACHARI_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8441710.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACHARI_000637"
     CDS             40412..41215
                     /locus_tag="ACHARI_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8441711.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACHARI_000638"
     CDS             41285..42025
                     /locus_tag="ACHARI_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8441712.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACHARI_000639"
     CDS             42036..43040
                     /locus_tag="ACHARI_000639"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8441713.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44883)
                     /locus_tag="ACHARI_000640"
     CDS             complement(43085..>44883)
                     /locus_tag="ACHARI_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441714.1"
                     /translation="RIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFY
                     TKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKI
                     LYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLI
                     SFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVS
                     EDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGV
                     LLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIH
                     CLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDL
                     LEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLC
                     SFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRET
                     HAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKD
                     VADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14427 a         7831 c         9344 g        13281 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc atagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gta
//
LOCUS       DAYYAO010000013        44459 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000013 DAYYAO010000000
VERSION     DAYYAO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.014"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..1141
                     /locus_tag="ACHARI_000641"
     CDS             230..1141
                     /locus_tag="ACHARI_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8441715.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1229..2146)
                     /gene="rnhC"
                     /locus_tag="ACHARI_000642"
     CDS             complement(1229..2146)
                     /gene="rnhC"
                     /locus_tag="ACHARI_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8441716.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2348..2785
                     /gene="zapA"
                     /locus_tag="ACHARI_000643"
     CDS             2348..2785
                     /gene="zapA"
                     /locus_tag="ACHARI_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8441717.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            2844..3392
                     /locus_tag="ACHARI_000644"
     CDS             2844..3392
                     /locus_tag="ACHARI_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8441718.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3507..5867
                     /locus_tag="ACHARI_000645"
     CDS             3507..5867
                     /locus_tag="ACHARI_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8441719.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            5954..6268
                     /gene="trxA"
                     /locus_tag="ACHARI_000646"
     CDS             5954..6268
                     /gene="trxA"
                     /locus_tag="ACHARI_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8441720.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6450..8228
                     /gene="uvrC"
                     /locus_tag="ACHARI_000647"
     CDS             6450..8228
                     /gene="uvrC"
                     /locus_tag="ACHARI_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8441721.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8373..9272
                     /locus_tag="ACHARI_000648"
     CDS             8373..9272
                     /locus_tag="ACHARI_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441722.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9265..10527
                     /locus_tag="ACHARI_000649"
     CDS             9265..10527
                     /locus_tag="ACHARI_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441723.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10688..12586
                     /locus_tag="ACHARI_000650"
     CDS             10688..12586
                     /locus_tag="ACHARI_000650"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8441724.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(12916..13635)
                     /locus_tag="ACHARI_000651"
     CDS             complement(12916..13635)
                     /locus_tag="ACHARI_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8441725.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    13825..13872
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            13972..14304
                     /locus_tag="ACHARI_000652"
     CDS             13972..14304
                     /locus_tag="ACHARI_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441726.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14291..16294
                     /locus_tag="ACHARI_000653"
     CDS             14291..16294
                     /locus_tag="ACHARI_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8441727.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16313..16783
                     /locus_tag="ACHARI_000654"
     CDS             16313..16783
                     /locus_tag="ACHARI_000654"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8441728.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            16804..17388
                     /locus_tag="ACHARI_000655"
     CDS             16804..17388
                     /locus_tag="ACHARI_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8441729.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17403..18389
                     /locus_tag="ACHARI_000656"
     CDS             17403..18389
                     /locus_tag="ACHARI_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8441730.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18379..18690
                     /locus_tag="ACHARI_000657"
     CDS             18379..18690
                     /locus_tag="ACHARI_000657"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8441731.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18712..20493
                     /locus_tag="ACHARI_000658"
     CDS             18712..20493
                     /locus_tag="ACHARI_000658"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8441732.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20486..21862
                     /locus_tag="ACHARI_000659"
     CDS             20486..21862
                     /locus_tag="ACHARI_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8441733.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            21869..22504
                     /locus_tag="ACHARI_000660"
     CDS             21869..22504
                     /locus_tag="ACHARI_000660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8441734.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22534..23889
                     /locus_tag="ACHARI_000661"
     CDS             22534..23889
                     /locus_tag="ACHARI_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8441735.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24081..25475)
                     /locus_tag="ACHARI_000662"
     CDS             complement(24081..25475)
                     /locus_tag="ACHARI_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8441736.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25567..26538
                     /locus_tag="ACHARI_000663"
     CDS             25567..26538
                     /locus_tag="ACHARI_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8441737.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26535..27317
                     /locus_tag="ACHARI_000664"
     CDS             26535..27317
                     /locus_tag="ACHARI_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8441738.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27486..27857)
                     /locus_tag="ACHARI_000665"
     CDS             complement(27486..27857)
                     /locus_tag="ACHARI_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8441739.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            27991..28434
                     /locus_tag="ACHARI_000666"
     CDS             27991..28434
                     /locus_tag="ACHARI_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8441740.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28477..29286)
                     /gene="yidA"
                     /locus_tag="ACHARI_000667"
     CDS             complement(28477..29286)
                     /gene="yidA"
                     /locus_tag="ACHARI_000667"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8441741.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29305..30672)
                     /locus_tag="ACHARI_000668"
     CDS             complement(29305..30672)
                     /locus_tag="ACHARI_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8441742.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30656..31486)
                     /locus_tag="ACHARI_000669"
     CDS             complement(30656..31486)
                     /locus_tag="ACHARI_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8441743.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31649..32086
                     /locus_tag="ACHARI_000670"
     CDS             31649..32086
                     /locus_tag="ACHARI_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8441744.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32162..32782
                     /gene="rpoE"
                     /locus_tag="ACHARI_000671"
     CDS             32162..32782
                     /gene="rpoE"
                     /locus_tag="ACHARI_000671"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8441745.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            32971..33477
                     /locus_tag="ACHARI_000672"
     CDS             32971..33477
                     /locus_tag="ACHARI_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8441746.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            33494..34393
                     /locus_tag="ACHARI_000673"
     CDS             33494..34393
                     /locus_tag="ACHARI_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8441747.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            34480..35364
                     /locus_tag="ACHARI_000674"
     CDS             34480..35364
                     /locus_tag="ACHARI_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8441748.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(35556..35627)
                     /locus_tag="ACHARI_000675"
     tRNA            complement(35556..35627)
                     /locus_tag="ACHARI_000675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35592..35594),aa:Arg,seq:ccg)
     gene            35687..37021
                     /gene="rpoN"
                     /locus_tag="ACHARI_000676"
     CDS             35687..37021
                     /gene="rpoN"
                     /locus_tag="ACHARI_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8441749.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            37170..38261
                     /locus_tag="ACHARI_000677"
     CDS             37170..38261
                     /locus_tag="ACHARI_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8441750.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            38302..39303
                     /gene="gap"
                     /locus_tag="ACHARI_000678"
     CDS             38302..39303
                     /gene="gap"
                     /locus_tag="ACHARI_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8441751.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            39413..40603
                     /gene="pgk"
                     /locus_tag="ACHARI_000679"
     CDS             39413..40603
                     /gene="pgk"
                     /locus_tag="ACHARI_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8441752.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            40707..41462
                     /gene="tpiA"
                     /locus_tag="ACHARI_000680"
     CDS             40707..41462
                     /gene="tpiA"
                     /locus_tag="ACHARI_000680"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8441753.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            41578..42876
                     /gene="eno"
                     /locus_tag="ACHARI_000681"
     CDS             41578..42876
                     /gene="eno"
                     /locus_tag="ACHARI_000681"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8441754.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            43275..44414
                     /locus_tag="ACHARI_000682"
     CDS             43275..44414
                     /locus_tag="ACHARI_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8441755.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        14753 a         7752 c         9478 g        12476 t
ORIGIN      
        1 ttaataacga agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa
       61 tcaccacgaa tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta
      121 gcagtaatgt tattttctac ggttatatct tttacagccc cttttttgct tacttaagct
      181 gattttaagt tttggccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat
      241 aattggagct tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt
      301 tggtcaaaaa aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac
      361 aaaggaatca tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg
      421 tacactatat acagatgact cgctaactac cccacttgca actataaacg gcggagagct
      481 ggcggagttt ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac
      541 tggatattta gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga
      601 agagctatct gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc
      661 attaagcaat gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt
      721 aaaaaaggcg ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt
      781 agtacagctt ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag
      841 tttacacgca gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta
      901 ctatggacaa gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct
      961 ggattcaaga ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc
     1021 tattttaatc gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga
     1081 cagttatcag cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta
     1141 ataaataagc ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt
     1201 atcgcagatg caagcttttg aacaccgttt atttttttag tagcttcaaa gctttttctg
     1261 tatttgcaaa gtgaagtttg gcatattttt caagcatagg catgccgcct ttctccaata
     1321 gttttgccgc tacttggtca gatttcgttc cagctcctga tggaagggtg attccagctt
     1381 cagctgatgc tttgtgtaac tcttccaaaa agctggcacg actgatgata gaagcagcag
     1441 caacagctaa atgatattgt tctcctttag taacgaagta cagtttttct tgtaattgat
     1501 ttttttctaa acgcgcatat tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa
     1561 tcgcttctgg tttttctggc tcgatttttt ttagcagcaa atggatcgct tgattgtgca
     1621 aagcgacttt catccgaacg gcattgtatt ttggttggat ctcattgtat ttttctggag
     1681 ataagacaag cagtttatag gggatggttt tttttaagat gtcagcaagc tggatgattt
     1741 ggctatcttt taactctttt gaatctctaa caccaagttt acgtaattct gcaagctgat
     1801 cacgtttcac ataagcagca catactgtta gcggaccgaa atagctgcca tttccgacct
     1861 catcactacc taaaacggat aattgactga tattttttgg caaagaagag gctgtttttg
     1921 ctggcttggc ttgtttatcc tgtgcctccc cccatttatc agcttctatt gctgcattcg
     1981 taccttgaaa catcactttt ccggatgtat aagcggtaat ggtcgtcccg tctttttttg
     2041 cagcaaagct agtataagga atctgtttat ttaacaaata agagcgatag tatgctttca
     2101 tttgactaag ttggtcgttt gttgctttga taatgacgtt gctcataaaa ttctcctcgt
     2161 ttttatacat aaaactatac cgcaactcac gacttttcgt tttctaagag attgcgagac
     2221 atagcatttt tttaaagttt atcatataat agttaagtat gtgaaaaata tttgtggaga
     2281 acggtgaaag aaccaacttc atggtacact aaataaaact ttcatcaaat aggaggaaat
     2341 gattcgtatg gcgcacgaaa aaacacgcta caaagcggtg attgccaacc aaacatatac
     2401 tattatcgga cgggaaacca agcatcatat ggatatcgta acgaagctga tcaatgaaca
     2461 gttagcagaa ttgaaacaat tatcaccaca aatggataat gaacaagcag caatcttgat
     2521 ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa agaatcttgg agctggaaga
     2581 agaaacagca gaattaaaga aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa
     2641 acgcattgaa gcaatcgaga acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga
     2701 tcataccatt aaaaatcatg tggaagcaca acagatttta aacgagaaac gaaaaggaca
     2761 aattaaacaa aaatcttcta attaagatcc ttttatcaaa ataagaaaaa tttaaaaaaa
     2821 gttagaagag aaaggaacgt agcatgctga gtttgctgat tttattttta ttgctaattg
     2881 cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat catattcgcg attggatatg
     2941 tattgtcatt tattgctgcc cagcattttt acaaaccatt agcaagtcat ttggaattat
     3001 acattccata cccagctgtc acgccaacat caaagttagc attttttgat caggtatttg
     3061 cctttcacct agatgaagct ttttatgcag gaactgcttt tctgattatt ttgctaattg
     3121 gctggctgct tacccgattt gtgggggttt ttgtccatgg attgacgtat gtgccgatct
     3181 tgagacaggt agactggatt gcaggaggca tcctgtcact aatcatggct tacgttacta
     3241 tatttttgat tttgcagtta cttgcctttg ttccattaga tgtggtccaa aatcaattcg
     3301 gcgagcatag tttcgctcgc tttatcgttg agagaacgcc atttcttacc aacaagatgt
     3361 atgagctttg ggtaacgaat attttgaatt aaggaaaata gtgctggaaa atatttcgtt
     3421 ttttgtacag agtagattat ttaagaaggc tgcgtagcgt atgtccggca cagctctttt
     3481 ttcatagtga aagataaggt gataaaatga ataaccgaat ccttgaaaca ttggaatttg
     3541 aaaaagtcaa acagatggtc agacaatttg tagtgactgc acaaggaaaa gaagaactgg
     3601 cagaattggt accagtgagc gaggaacaaa cgatcacaaa ctggcttcaa gaaactgagg
     3661 atggattgaa agtgcaacgt ctgcgaggcg ggatccctat acctaaactt gaaaatatcc
     3721 gtccccatat gaaacggatt gagatcggag cagacttgaa tggagtggag cttgcacaag
     3781 tttccagagt attgtctacg acaagcgaat tgaatcgttt cattgatgat ctttatgaca
     3841 gtgaaatcga atttgcacgt ttgtatatgt gggcagatca gttagtcacg atccctgttt
     3901 tgagccgccg tttaaaggaa gcaatcgatg aagacggacg cgtgactgat gacgcttctc
     3961 ctgagttaaa gagcataaga cagaacattc ggcgaagtga acaagctgtt cgtgaacagt
     4021 tagatgggat cgtccgtggg aaaaatgcaa agtatttaag tgatgcgatc atcaccatgc
     4081 gaaatgaccg ctacgttatc ccagtcaaac aagaataccg cggtgtattt ggcggtgtag
     4141 tccacgatca aagtgcttca ggacagactt tatttattga accaaaacaa gtggtggatt
     4201 tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa tgagattcaa cgtattttaa
     4261 gtgaactttc tgctgaactt gttccccatc gtcaagaaat catccacaat gcttatgtga
     4321 ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg taaagaagta aaagctatcg
     4381 tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca agcaagacat ccactgatcg
     4441 atcaggaaaa agtagtttct aatgacatca caatcggaaa agactatcaa gccatcgtca
     4501 ttacaggacc taacacaggt gggaaaacga tcacgttgaa gactttaggt ctgttacaac
     4561 tgatggggca agcaggatta ccgattcctg caggtgaaga aagtcaaatc ggtatttttg
     4621 aggaagtatt tgcggatata ggagacgaac agtcgattga gcaaagtcta tcgacctttt
     4681 cttctcatat gacgaacact gtagatattt tatcaaaagt caatgaaaaa agtctggtat
     4741 tatttgacga gctcggtgca ggaacagatc cgcaagaagg tgctgctcta gcaatcgcaa
     4801 tcttagatga tcttggtaaa aagtctgctt atgtgatggc aacaacgcat tatcctgaat
     4861 tgaaagttta tggatataat cgggcaaata cgattaatgc cagcatggag tttgatgtgg
     4921 atacattaag tccaacgtat cgtttgctga ttggcgttcc tggacgaagc aatgcgtttg
     4981 aaatctctag ccgattaggt cttgatacag aagttattga tgaagcgaag cagttgatga
     5041 atgatgagag ccaagattta aacgaaatga tcacagattt agagaatcgc cggaaaatgg
     5101 cagagaccga atatctggaa atgcgtcatt ttgtttctga ggcacaagaa cttcacgatg
     5161 atttgaaaga agcttacagc tatttctttg aagaacgtga aaaagaaatg gaaaaagcaa
     5221 agaaaaaagc aaatgaagtc gtgtcagagg cggaagaaaa agcagaaaaa atcattgctg
     5281 atattcgtaa aatgcaacag cagattgggc aaggaaatgt gaaagagcat cagttgatcg
     5341 atgcaaagac ccaattagca aatctacatc aagaagaaac tttgaagaaa aataaagtat
     5401 tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga cgaagtgttg gttacgactt
     5461 acggccaaag agggacgctt ttacgtaaaa acgggaatca gtggcaagta gaaatcggta
     5521 tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt tgctcctcaa aaagagccga
     5581 cacaacgagt tatccatgct gtacgatctg aatctagtag tcacgtgccg aatcaattgg
     5641 accttcgagg aaaacgatac gaagaagcgc taagtgaggt agatcaatat ttggattctg
     5701 ccattctagc tggttatcct caagtgacta ttgtccacgg aaaaggaaca ggcgcacttc
     5761 gcaagggaat tactgattat cttaaaaacc atcggagcgt caaaagcttt gagtttgcac
     5821 cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt taagtaaagc ttactaaagg
     5881 taagcagatg gttcgctatc caagtataga aatgctatac tagtcataga ttttaaaaat
     5941 ggaggtcttt gatatgtcac aagtaattac agataagaca tttaatgaag aaacagataa
     6001 aggacttgta ttgattgatt tttgggcaac ttggtgtggt ccatgtcgta tgcaagcacc
     6061 gattttggat cagttggaac aagaatatga tgaagaagaa ttcagaattg ctaaaatgga
     6121 tgtggatgaa aatccagaaa ctcctcaaca attcggtatc atgagcattc caacattaat
     6181 gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt gttcattcaa aagaacaact
     6241 acgtcaaatg atcgatcaat atttataaaa acttacagca aaaataagct gagggtggga
     6301 ctgaatccag ttccgtcctc ggtttttttg cggttgagtt aaggttattc aaattaatat
     6361 tttttgagta taattacgtt gtcaggctgg attgtttttt tgacagacat gctataataa
     6421 ctttaattga atatgaaggg gagtaccaga tgaacgaacg aatcaaaaac aaattagctt
     6481 tacttcctga tcagcccggc tgttacttga tgaaagataa aaatggaacg atcatttatg
     6541 tcggcaaagc caaaatatta aaaaatcgtg tccgctcata ttttactggt agccataata
     6601 cgaaaacgga acggcttgtt agtgaaatcg aagactttga atacatcgtt acagaatcga
     6661 atatcgaagc attgctctta gagatcaatc tgataaagaa aaatgatcct aaatacaaca
     6721 ttatgttaaa ggatgataaa acctatcctt tccttaagat aacgaatgaa aaatatcccc
     6781 gtttggtgat tactcgtaag gtactaaaag acaaagcact gtattttgga ccatatcccg
     6841 atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg attatttcct ctgagaaaat
     6901 gcaaacctag tcagacgaag gagccttgtc tttactatca tttgggacaa tgtctgtgtc
     6961 cttattattt cgacgtagat ccgaaagtct atacgaaaat cgttgatgaa gtgaaaaact
     7021 ttttaaacgg aggacacgaa aaaatcgagt cagagatcca tcagaaaatg gaaaaagctg
     7081 ctgaaaacat ggagtttgaa aaagctgcag aataccgaga ccagatccgt gccattgaga
     7141 caatcatgac tcgccaaaaa atgacaaata ctgatttact ggatcgagat gtatttggtt
     7201 atgcgattga taaaggatgg atgtgtgtcc aagtgttttt cgtaagacaa ggaaagctaa
     7261 ttgaacgaga tgtttcaatg tttcctttct ataacgaagc agaagaagat ttcttgactt
     7321 acctcggtca gttttatcag gaaaatgagc atttcattcc taaagaagtc ttgattcctg
     7381 atacgatcga caagcccagt gtagaagctt tgttagctac gaaagtgctg caaccaaaac
     7441 gaggagaaaa gaaaaaatta gttgaattgg cgagtaagaa tgcctcagtt gctttgaatg
     7501 aacggtttga tctgattgcc agaaaacagg aacggacgat cggagcagtt gaacgtttag
     7561 gaaacgcgat gaatatcccg accccaatac gtatcgagtc ttttgataac tctaatatca
     7621 tggggacgaa tcctgtggct gcgatggtcg tattcattga tggaaaaccg gcaagaaaag
     7681 aatatcggaa atataaaata aaaacagtca acggaccgga tgattatgca tcaatgagag
     7741 aagtcatcta tcgcagatat tcgcgagtga ttcgcgaagg gttgccttta cctgatttga
     7801 tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa agatgtctta gaaaatcaat
     7861 taggaatcga tattcctatt gctggcatgg caaaaaacga taaacataaa acaaatgagt
     7921 tgctattcgg tacagatttg gctgtagtac cgcttgagcg aaattcacca gagtttttct
     7981 tactccagcg aatccaagat gaagtccatc gattcgcaat cactttccat cggcagctac
     8041 gcagtaaaaa cagttttgct tcccgattag atgaaattga agggttagga ccaaaacgaa
     8101 aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat cacagcagcc acgacagaag
     8161 aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa cgtatttgat aaattacatc
     8221 aggaatgagg aaagggcact ttacaaagaa agtaaagtgt cctatcattt tatgtacaac
     8281 aaaaagagcg tttacgtttt gttggtaaat aataaatcgc ttacgtctgg tgtctaagga
     8341 tatatgtgat ttcttgaaga aggagtgaag aaatgttaga agtcaaggat cttgttaaaa
     8401 cttttgggaa ctacacagct gtcgatcatg tctctttcca aatccccgat ggaaaaatca
     8461 tggggctaat tggccaaaac ggggctggga agacaacgac ttttcgtttg atcttggatt
     8521 ttttgacaca agaccaagga gaagtgttat ggaatggcca tcatcttggg gagaaagatt
     8581 atgatgtcat cggttatctt ccggaggaac ggggacttta tccgaaagta acgattcaag
     8641 accaactgct ttattttgca gaacttagag ggaaatcaag aaaagagatt gaaccaaaaa
     8701 tcgacttttg gatggaaaaa tttcaggtaa aagggaaaaa gacggataaa gtaaaatctt
     8761 tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac gttaatccat gaacctaaat
     8821 tgatcatttt ggatgagcca ttcagcgggt tagatccggt aaatgctgaa ttgctaaaag
     8881 atggaatatt ggaattgaaa aaacatggtt catgtgtcat tttttctagc cacaatatgg
     8941 ataatgtaga aaaaatctgc gatcatttga tcatgttgcg aaacggaaaa atggttttga
     9001 atggaaaagt acatgagatt cgtgaatcat ttggccgtac gaagttattc ttggaatctg
     9061 gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa aaaagtgatc cttcatgaag
     9121 acaatacgtt agagattacc ttagaagatc ctgaagctgg gaaagaaatc tttactcgtg
     9181 cgactcagtt cggctacatt cctatgttta accagcaacc gccaactttg gaagagattt
     9241 tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga ttattaccaa agatgtctat
     9301 ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat tagtcccttt tatcatgctg
     9361 ggtatcattt atgtagcagg aaactttgct caacaaaata gtgatgtcaa taaaatcggc
     9421 gtattggctg ataatgagca aatcagtcag caaatgagcc aagtcaaaac agatgatttt
     9481 gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag agctttcgga agaaaaaatc
     9541 gatgcgtatt tagtagtctc aactgagaat gataagataa atgggatgct ttatagcgaa
     9601 aattcattag gacaaagtac acaactactg atacaacaac aactgactgg cttgcaatcg
     9661 atgcttcgag cgagtcaatt aggcatttca ccagaagaag tagctagctt gagccaacaa
     9721 gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag gaaaaatgac agtcggagaa
     9781 gataactcga cagtccaata cgtcgtaagt tatgtagcaa cgattgtctt gttcattatt
     9841 attttgacgt atgcgcagat cattgcacaa gagatcgctt ctgaaaaagg cacgcggatc
     9901 atggaagtga tcctgtccag tacgactgca caaaagcatt tttacggaaa attaacaggt
     9961 gttttacttg ttgcattaac gcaaatggca ttatacggta ttatttttgg tataggatac
    10021 agtcaattca aagatatgga aatggtcaaa aatatattag acggtatctc attggataac
    10081 ttgattggac cattcttgtg gtattctctt ctattcatga tctttggcat tttgattttt
    10141 tctgttttag cagctttatg tggctctttg gtcaacaaag cagaagatac agcaaaagca
    10201 attttgccag tgacttatct atctctaggc ggatacatgc ttggattgat actaggagct
    10261 tcagatccaa acaatatcgt tattcggatc acttcgtata ttccattctt atcttcatat
    10321 attatgccgg ttcgcttagc aaatgagaca gtcggtatgg gtggagcatt gatttccttg
    10381 ttggtacttg tagccgtaac attcttattg atgatcttgt ctgcaaacat gtacaaatcg
    10441 aatgtactcg tttatagcga aggcggatta tggagttcat tgaaacaatc gatttcaatt
    10501 atgaggaatg aacgaaaaaa agcataaaac aatcctaaaa tacagaagga agctggacat
    10561 ctattgttcc cagcttcttt ttgtatagac ctattttatt tattttacta tattggatat
    10621 atattcattg aaaatagtgt ataacaactt tataatattg agtataccaa tttttggagg
    10681 cgttttaatg gaccgttatt tagatttact ttcagaaaaa ttcccctcaa cggaggatgt
    10741 catcacggaa atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt
    10801 tttgagtgat atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg
    10861 aaatcttaaa gaaaagatca aagacctttt ttctgaacaa atgacggaaa cggatatcag
    10921 ccaattcact atttttacgg cttatccaga atatgcatta acgactgatt ggtacaaaga
    10981 acaagataaa tcgcagctga tccatcattt gatcgatctt ttacggttta cgacagtcaa
    11041 atatacccgg tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga
    11101 actcttgtat atcgatgacc gtatcaatgg aaaaaaagac tatgtaaaaa aaatcattga
    11161 acaacttgtt ttgatgaagg aagaagaaaa atttttaacg aaacttgcac agacgatcca
    11221 aaaatctgtc atcgatcatt tgcatatcgt tggagatatt tttgaccgag gaactcaagc
    11281 agccaaagtg atggatcgaa tcatggattt ttcttctgta gatatccaat ggggaaatca
    11341 tgatattttg tggattggtg cgtattgcgg ctctgaggca tgtttactga cacttttacg
    11401 gattgcagca cgctataatt atttatttga attagagaaa gaatatgggt tgaatttacg
    11461 tccattgttt tcttttgctc atcaaactta tcagaagaat ccagtatttg cgcctaaaaa
    11521 cagtaaaaat ctatccgaaa gagagcaaga agaattagaa aaaatccatc aggcactttc
    11581 tatccttcaa ttcaaatcag aacatcagtt attggatcgt cgacctgaat ttaaaatgga
    11641 cgatcgtaaa ttgctggaaa aaatcgacta tgaagcatca acgattgatt tagaaggtca
    11701 gctctatgag ttgaaaggaa cttgttttca aacgatccag ccagatgatc caaaaaaatt
    11761 cttggaagag gaacaaaaag tgattgatag tttgatgtat tcattccagc actctttgag
    11821 aatgcaaaaa catatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca
    11881 gcatgtgctg tttcatggct gtatacctgt agacgagaag ggacagctct tagcatttga
    11941 attagaccaa tcttatcgcg gaaaagaact gctgggcttt tttgaaaaac agataactga
    12001 aagcgcaaaa gatctgactg caaaagaaga tttagcaacc gatttgattt ggtatgcgtg
    12061 gagtgggcca ttttcgcctt tattcggcaa gagtaaaatg gcgacttttg aacgttattt
    12121 tctcacagag cctcatacgc acgtcgagaa aagcaatcct tattatcact tgcgagatga
    12181 agagtggttc agtgaaaaaa ttttagcaga attcgacaca aaagaagaag ggtcagcaat
    12241 cattaatggg cacacgccag taaaagtaaa aaaaggggag tcaccgatca aagcaaatgg
    12301 aaaagtattt gtcattgacg gaggtttatc caaagcttat caaaaaacga caggaattgc
    12361 cggttattca ctattatgca attcttatgg tttccaaatc gtgacacatt atccgttttt
    12421 gcctattgaa caacaatttg caaaaaaatc cgaccaaaca aatgtgaaaa aagtaatcga
    12481 acagcaacta ccacgcaaac tcaatcgtga tacaacaaaa ggtatggaac tgcagcagca
    12541 gattacccag ctcaaaaggc tgttggacta tcgaaaggac gattgagaag aattccagtg
    12601 aacattgttc catagactgg agactgagac aaaagtcgtg tcgcagtctc tttttccgga
    12661 taaacggtgt tcttctgcca actcctcggt cacaagtcac actattcaac aaacatgaaa
    12721 agctgttttt tgaaattgct ccttgtgact cagagttgaa cggcaggctc acgacctctt
    12781 ggtataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt
    12841 gaaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacagctt tttcacaacc
    12901 ccctcagaaa gtctattaaa attcattcac aaagaaatcg aataagcgct gttcagatgg
    12961 atgtacacgg tgggtaagtt ccggatgcca ttgaacaccg aggattttat gcatatcatc
    13021 tttgctttga accgcttcaa tgagtccatc gttagaaaaa gcgatggctt ttaataaagg
    13081 tgaaagatct ttgactgcct gatgatggta agaattcaca aaatatggat cggggagaag
    13141 gttgcttaac aaactgtctg aaacaatctt gacttcgtga gtcgtaaatt gcggggcagt
    13201 gggctgttgt tcatgtttta ctgaccactt tggatataat gaaagatctt gataaagtgt
    13261 accttccaaa acgacattca atagttggag tccccggcag actgcaaaaa ttggttttct
    13321 ttgtttcaat gcttccaaga tcagtgcctg ttcaaattgg tctcgttgaa tatttgtttc
    13381 cgttagcttt ggatgaggat cttccatgta aagttgtgga ctgacatctt gaccacctgc
    13441 aagaataagt ttgtcgattt gtccaatcag ttgaggagct gaatctggag acatgactgg
    13501 cagaataaga ggcaatcctt gagcatctaa aactgcatcc acgaatcctt gcggtgtata
    13561 gctcacttga tttccttgga aagtatctgt tgctcgaatc agttgatttc cggcaatgcc
    13621 gatgattggt ttcatttgta ttccccctaa agtagactta ttgtaacaag gatttaaaaa
    13681 aaattaaact atgtaagact tgcccctata cttatctgtt cttgctgtgt tactatctgt
    13741 aaagagaaat ttcgtgtgaa attcatgacg atagttgaaa atattgctta atttgttata
    13801 ataaaaagta agcagaaata aaaaggttgg gcgtaagctt caaggatcta gttcttcaag
    13861 gggtgagaag gacattttat tgaaggggcc tgcctatact aagttatctt tagaggtaca
    13921 tactttcaat gaaaaaagaa agcatgtacc tcttttttaa atcgaaagga agtgaagcaa
    13981 gtgttagaag ctttggaaca aatccgaaaa gcagaagaag aaaatgaaaa gcgtgctcaa
    14041 acactgcggc agcagctgaa gatctatgca acagaaaaag agcaggaatt ggaaacgatc
    14101 caaacaaact atcgacaaca actaaaacag ttggtggaag aaaaagaaca atttgaaata
    14161 caagaattag aaaaagaaaa aatgaaattg aaagcagaag cagacaaggt gcaagcaact
    14221 ttgaaagatc aatatcaaaa gcatcagagt caagcgatcg gtgcgatcat tgaaagggtg
    14281 aaagaaactt atggccgtca ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa
    14341 tcaggagagg attttacaag cagtacaagg aatccaaaac gttgaaatcc gggatctgta
    14401 tcaaaataga acaaacaacc agtgggtgga gaagtatttt ccagatacca cgattattga
    14461 taaagaagca aaattaagcg ctttaggtaa tcggttgaaa gacatccaag aagctatcca
    14521 attcatacaa caccacggcg atagccgtca aaagaaactc catttgaaac gtcaagaact
    14581 ttccttacaa gatttggaag ccaattattc cgaagaaaat ttcataaata aattgaaaga
    14641 agtccttgat ttaaaagaaa agtgggaaca actaactgaa gaacgtgatc gtttagaaga
    14701 agaggaagaa tggcttcttc attggcaaca gctggatatt gctccaagta tgtatgcaag
    14761 taagacgacc atttttgaga tggggactgt gagtgcagca aattttgaaa gtttcaagga
    14821 agaactgagt aagcttgatg acgtttatat ggaagaaata gattatacgc caaaacaagt
    14881 ttatctttcg tatgttgctt taaaaaaatc agttgctgtt atagaagaaa ttgctgggaa
    14941 atacgggttc aataaagaaa cgtatcctta tgaacaaaat cctaaagaaa aactgcaaga
    15001 gaccaagcag caattatcca ttgttcacga gcagcaaaag aagataacga cagcgttagg
    15061 tcagtgcagt ggctatatcc aagaatttga atgggtagaa gaagtcacac ttgccgttgc
    15121 agaacgagaa aaaataaaag aacggtttat ccatgcgact tatctgatcg ttttgcaagg
    15181 ttgggtagat gcagaagaaa aacagaattt gctgcatgtg ttaaacgaaa ccgtatcaga
    15241 agaagatata tacgtcagtt tcgaaacacc aaactcagaa gagattcagc aggaagtccc
    15301 tactaaattg aaaaaccatc cgcttgtcga accttttgaa ttgctgactg aaatgtacag
    15361 tctgccaaaa tatgaagaag tagatccaac accttggatg acaccatttt atcttgtctt
    15421 cttcgggatg atggtcgcag atgttggtta tggtttgctg atgctcattg ggacaatact
    15481 agctcaaaaa ctacttgttc taccacgcgg gatgaaacgt ttcgtaaaat tctttgagat
    15541 tctctcgata ccatcgatca tctggggatt gatctacagt tcatttttcg gacagtcatt
    15601 accaaaagaa ctgtttggca tccgcttgcc tttcccaatc ctatctacga cggatgacgt
    15661 caatacgatt ttgattttat ccgtgatttt cggtctgata cagattttgg ttggattatt
    15721 tgtggcagca aaggaaaata tcaggagaaa agattacttg agtgctgtca gtgatggatt
    15781 tgcctggcaa gggatcctaa ttgggatcgt tattgcgttg gttggtgcca tgttgataaa
    15841 aaatacagca tttgtttacc ttggcgctgg gctatcgatc attgctgcct tatgtatctt
    15901 gatcgttccg attatccaaa cgccgtctaa agtcaaaggt gctgccaaag gtgcgtataa
    15961 tctttacgga ctgaccggct atatcggtga tttagtcagt tatacacgtt tgatggcatt
    16021 gggtatttca ggcggaagta ttggagcggc attcaatatg ctagttgctt tcatgccgcc
    16081 agctgctcga ttcagtgtag ggatcctttt gatcatcgct ttacacgcac tgaatatgtt
    16141 tttgacactg ctaagtgcct atgtccatgg cgcacgattg cagtatgtcg aattttttgg
    16201 gaagttttat acaggaggcg gacgtgcctt tgatccgctg aaaacggcgg aaaaatacgt
    16261 caacatcaat cacaagaaaa aaaacaataa ataattggag gatttttata taatgatgga
    16321 ttacttaatt agtcaaaatg gtggaatggt atttgcggta ttagcgatgg caacagcgac
    16381 gattttttca gggatcggtt cagctaaagg tgttggaatg acaggtgagg cagcagcagc
    16441 attgacaaca agtcaacccg aaaaattcgg gcaggctctg attctgcaat tgcttccagg
    16501 tacgcaaggt ttgtatggat tcgttattgc ctttttgatt tttatcaact taggaaatga
    16561 catgtcagtt gtacaaggac tgaactttct aggtgcttct ctgccgattg cttttacagg
    16621 cttgttttca ggtattgccc aaggaaaagt cgcagcggca ggtattcaaa ttttagcgaa
    16681 aaaacctgaa catgctacaa aaggaatcat ttttgctgcg atggttgaaa catacgcgat
    16741 tttaggtttc gttatttcct tcctattagt gttgaatgcc taagcataaa ggaggaaaag
    16801 cagatgaacg ccattgaaaa aatcatctct cagatgaacg aagcagcaga gcaggaaaga
    16861 gcggcgttag aacaagaaga gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg
    16921 caagttgaaa cagaacatca aaaacaaaaa gaaaaacaaa tcgaattact cgaaaaaaaa
    16981 taccgtcagc taaggaatcg ccagcaagta gaagtacgac aagaaaattt gaatgcgaag
    17041 caggaatttt taagacgatt gtttgcagat gcggtaactg aaatggaaaa ttgggatgaa
    17101 tctgaacaaa tccagtttat caaaaatgca ttgtattcat tacctttgac aggaaaagta
    17161 gcattcattg ctggagaaaa atcagcagcc tatctttctc agacgttgtt ggatgaatgg
    17221 aacaatgaac ttccctttat gatggtttta agtgatgaaa ctgttgctga tcaagcgggt
    17281 tttctgataa acgatcaagg agttcagtat aattttcttt tcagcagcct tgtacaagat
    17341 atccaaggaa cgatgagttt tgagattgcc aatcaattat tcgaataagg agggatcagt
    17401 gtatggaata ccacgaacta aatcccttga ttcgcggaag agagcttgaa cttttgtcca
    17461 atgagacatt tgaacggatg attcaaacaa attctgtcga agcacttggt gaaatattga
    17521 aatccacgat ttacagtcca tatatttatg acggatatga atatgacttc gaacagaatc
    17581 ttttaaagga gcagagccgt ctatttcgct ggttaaagga atcagcaccg gagcctgaaa
    17641 tcgtttggat ctatacgatg cgttatactt tccataatct gaaggttttg acaaaagctg
    17701 agatgacaaa tcaaaatctg gaccatttgt atatcgacga tggattttat tcattagaaa
    17761 ccttgaagga tgccattcat acgcaagttt caaatgaact tcctgaaagc attatggaca
    17821 gtatacgaga agtacgtgat tattttgaag agtctaccat tcttcaggga attgacgtga
    17881 tttatgatcg acagtttttg acagagcagc gacggcttgc cgataagcta ggatatcaag
    17941 aattgctgac agaagtcatt gcttttattg acttgaccaa tatcacaacg atggctcgag
    18001 gaatattgca gaaaagaaca tatgggttca tgacgacggt actttccagc tcgggaagta
    18061 tcgaaaaaga agtatttttg aactttgtag ataaagatat ggaagattac acgaattttc
    18121 tattagagac agattatgct gattttctgc gccctgtcat ccgtgaggga aatattgatc
    18181 tcgtaaagct tgaacagttg aaagatgatt atctaagttc attgtatcaa gaagcacaaa
    18241 cccaggcttt cggaccactg ccactactag cctttttgaa cgcaaaagag gtagaaagca
    18301 aaaaccttcg tttactggtt atcggcaaaa gaagccattt cgaatcagaa acaatcagaa
    18361 agcgggtgag acaagtctat gacgcataag atcggtgtag ttggcgacaa agattctgtt
    18421 ttgccattca aattgtttgg ctttgatgtt cgttatggta caaccaagca aaccgttcgt
    18481 caggcaattg aagaaatggc aaagaatgag tatggcgtga tttatgtcac agagcaatgt
    18541 gcagcattga gcccagaaac gatcgaacgt tacaaggaac aaatgatacc tgctatcgta
    18601 ttgattccca atcatcaagg atcattaggg atagggctgg aagaaattca aaaaaacgta
    18661 gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg agatagaaat tttgcaaatt
    18721 ggaaaaatca taaaagtctc cggacctctt gtaatggctg agaacatgtc tgatgccagt
    18781 atccaagaca tgtgtctagt gggagatcta ggagttatcg gagaaattat cgagatgcgt
    18841 ggcgatgtgg cgtctattca agtatatgaa gagacttctg gaattggtcc aggcgaaccg
    18901 gttcgctcta ctggtgaagc tttatctgtt gaattaggac cagggatcat ttcacaaatg
    18961 tttgacggaa tccaacggcc attagatacc tttatggaaa tcacacaaag taatttctta
    19021 gggcgcggag ttcaattgcc cgcattggac catgagaaaa aatggtggtt cgaaccaaca
    19081 gtagaagccg gagaaacggt gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa
    19141 gttataaaac ataaaatcat ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa
    19201 aaaggttcat tcactattga tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa
    19261 aatttgacga tgcttcaaaa atggccagta agacgtagtc ggccaatcaa agaaaaatta
    19321 aacccggatg cacccatgat cactggacag cgagtgattg atacattctt cccagtgaca
    19381 aaaggaggag cagcagctgt acctggtcct tttggtgcag ggaaaacagt cgttcagcat
    19441 caaattgcaa aatgggccga tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga
    19501 aatgagatga cggatgtttt aaatgaattc cctgaattga ttgaccctaa tacaggagaa
    19561 tcgttgatgg aacgtacggt attgattgct aatacatcaa acatgccagt agcagctcga
    19621 gaagcttcta tctatacggg aatcacaatt gcggaatatt tccgtgacat gggttatgac
    19681 gttgctatca tggcagactc cacttcacga tgggcagaag cacttcgtga gatgagcgga
    19741 cgtttggaag aaatgcctgg tgatgaaggt tatccagctt atcttggttc acggcttgcg
    19801 gaatactatg aacgatctgg aagagtcatt gcattaggat cagaaggtcg cgaaggaagt
    19861 ataaccgcaa tcagtgctgt atccccgtct ggaggggata tttcggaacc agtcacacaa
    19921 aatacattgc gggtcgtaaa agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga
    19981 cattttcctt cgatcgattg gatacaaagc tattctcttt attcaacaga agtagggaag
    20041 tatatggatc atatccttca agctgactgg gcatccatgg tcaccgaggg catgcgtatt
    20101 ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg tagggattga ttccctttca
    20161 gataatgacc gtctgacatt agaagtggca aaatccattc gtgaagatta tttgcagcaa
    20221 aatgcttttg atgatgtcga tacattcact tcaagagaga aacaattcaa aatgttgaag
    20281 atgatcttga cctttggtga agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt
    20341 gagatcatgg acggtaccgt atcgattcgt gaaaggatcg gacgaagcaa atatatcccg
    20401 gaaacagaat tagaaaaact agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg
    20461 atcgtttcag aaggaggcat gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt
    20521 cggtccattg atggcggttg aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt
    20581 ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg gaagtccaag aagataaagc
    20641 aatggtccaa atattcgaag gaacaagtgg tatcaattta cgtgactctt ctgtacgatt
    20701 tttaggccat cctttagaac taggtgtatc ggaagacatg atcggtcgag tattcgatgg
    20761 tttgggacga ccgaaagata acggaccaga gatcctgcct gaaaagcatt tggatattaa
    20821 cggagaagta atcaatccag tagctcggga ttatccagat gagtttatcc aaacaggtat
    20881 ctctgcaatc gatcatttga atacattagt tcgtgggcaa aaattaccag tattttctgg
    20941 ctctggtctt cctcataaag aattagctgc tcagatcgcg cgacaagcaa cggtactcga
    21001 ttcatcagat gatttcgccg tagtctttgc tgctattgga atcacttttg aagaagctga
    21061 atttttcatg gaagattttc gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa
    21121 tttagcaaac gaccctgcaa tcgaacgtat cgctactcct cgtatggctt tgactgctgc
    21181 agaatatctt gcttatgaaa agggaatgca tgttttggtt atcatgacag acatgaccaa
    21241 ctatgcagag gcattgcgag agatttctgc agcacggcga gaagttccag gaagaagagg
    21301 atatcctggg tatctttaca cgaacttagc tactttattc gaacgagcag gaagaatccg
    21361 cggactaaaa ggatcggtta ctcaaattcc gattctaaca atgccagaag acgataaaac
    21421 tcatccaatc cctgacttga ctggttatat tacagaagga cagatcatct tgactagaga
    21481 gctttacaag agtgggattc agccaccaat tgatgtactc ccgtctcttt cgcggttaaa
    21541 agataaggga actggacccg gaaagacaag agaagaccat gcagctacga tgaatcagct
    21601 ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta gccgtagttt taggtgaatc
    21661 tgctttatct gatgtagaca agatctatgc gaagtttgct gagcggtttg aaaaagaata
    21721 tgtcaatcaa ggattctaca cgaaccgttc gattactgaa acacttgatc tagggtggga
    21781 actgctatcg atgttgccaa gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa
    21841 atatctgcca gaagggaagt gagttctgat ggcacgattg aatgtgaatc cgacccgcat
    21901 ggaattgact cgattgaaaa aacagttgac aactgccaca agagggcata aattattaaa
    21961 agataagcaa gatgaactga tgcgtcaatt catcctgttg attcgaaaga acaaccagct
    22021 gcgtcaagaa atggaagagt ctatgacaga agcaatgtct gattttgtat tggcaaatgc
    22081 ttcgatcacg gaacctttca tcgaagaatt atttgttttg ccggcggaaa acgttgattt
    22141 gtctatcgta gacaaaaata tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga
    22201 tgaacagctg acagacacac ctttggaata cgggtatttg aattccagtg ccgaactgga
    22261 tcggtcaatc gatcggttta ctaccctgct tccaaaactt ttggaactga cagaaatcga
    22321 aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt
    22381 agaatatatg acgatccctc aattagaaga aacgatctat tatattcgta tgaagctgga
    22441 agaaaacgag cgcgcagaag taacaaggtt gatcaaagta aaaaatatgg gtaccactga
    22501 ctaagtggta tcttttttta aaagaggaaa acgatgatta aacaaagcaa acaaataaag
    22561 aaacgtttgc cggctgttca gcttctcgca ttaggatttt ttctcctgat tttagttggc
    22621 ggcggcctac ttaccctacc attttttagt aatagtgggc atgggacaaa ttttatcgat
    22681 gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat tgacaacatt gaatacagca
    22741 gagcattgga acagtgctgg tcaatttttg atcatgcttt tgattgagat cggcggtctt
    22801 ggttttatga tggttccgat tctatgcttt gcattgataa agaagaaagt cagtctcagc
    22861 acacgtatta tattaaaaga agcgctgaat ctagaagaag tatcaggtgt gatgaagctc
    22921 atgctttaca tcctaaaatt ggcctttgtg atccaattga ttggtacggt tgctttatct
    22981 tttgtatttg tgcctgaatt tggctggttg aaaggtatct ggtacagttt gttccatgca
    23041 atctctagtt tttgtaatgc agggtttgat ttgttaggag acagtttagc caatcaccag
    23101 caaaatgttt atttgatcat ggtggtatct gctttgatca tttctggtgg gttaggcttc
    23161 atcgtatggc gtgatttact ggaatatcat cagaaaaaga agatgacgat tcattcaaaa
    23221 atcgcattaa ccgttacagg taccctttta ttaggcggat tcgttgtgtt ctttttcaca
    23281 gagcgcaatg gggcaacgct agtggaaggg aatatgtttc aacgattggc aaacaccttt
    23341 tttatgagtg tgacacctag aacggctgga tattattcag ttgattacct ccatatgagc
    23401 catgcaggtc tgattttaac gatgtttttg atgtacatcg gcggaacatc tgggtcgacg
    23461 gccggcggat tgaaaacaac tacgttaggt gtgttgctga tccagatgcg ttcgatgttg
    23521 aaaggacgta cgagagcgga agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg
    23581 gcacttactt tattttttgt tacgttgacg ttgtgtgtag tcgccatcat gattttatcg
    23641 gtgactgaaa cgatacctaa aacttctggc atcgaatata ttgcttttga ggtcttctct
    23701 gcgtttggta cagtaggtct tacgatggga ttgacgccag acttgactgc aatagggaaa
    23761 ttgatcatta tagcgcttat gtacattgga cgagttggtg tattgactgt ggtcttctct
    23821 ctgatgacaa aagcacaaag aacacaatca aaatacaaat atccagaaga aagtgtcatg
    23881 attggataat ttcatcttct gacagaaacc tagtgattta gtaaaataag aaaataaaat
    23941 aatttttctt gcttcttgga tactttaagt gcaaaatgtt cgtttatttt ttgaggcact
    24001 acccgttgtt gaacctcggg tagtgcctct tttttattga aaggaatcaa taagatttga
    24061 ttcctttatt tatcaatagg ttataattca tattttttta ttttttcttt tacgatactt
    24121 agtccagaag ctaaaatttc aaggttatta gaggataggg aaattcgaac gaatgcatct
    24181 ggttgcgtac gtgtagtaaa gcgtctggaa tgataaagtc ggataccgtt agcaagaaag
    24241 tcattctcaa ttatcttagg gtcaatctca ggatcaactg gaagaattcg atagtaaggg
    24301 acacgattcg ttggtttttc tactttgaaa agagaatcaa aaagctgatt ggctcttttt
    24361 gtaagactta atttagtttc taagatccta tttgcttcat ttgaatggag gatttgagtg
    24421 atgacttcca catctaatcc agaagttttc acatttatat taaaaattgc ttgttctatc
    24481 ttgctttgga catgtactgg aactacaaga taagcaactc gcaatccaga acaaataaat
    24541 tttgtcattc cagctaagta gattgtttgg ttaggtaata aattttggaa aggaggaatg
    24601 atcgtatgtt gatagtaagt tgttaaaaat gaatggatat catcttcaat aacccataga
    24661 ccttcttttt caatcacttc ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct
    24721 ataggattat tacaagaagg cataagaaag acaccatgaa tcttcttttt tctacattcc
    24781 tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat caattggaac gatctctaaa
    24841 tgatataact gagccaattc aatgaaatta gcgtatgtat atcggtctgt tgctattcta
    24901 tcacctggag agaaaagtgc agaaagtgtg atagccaatc cattttgaac accggaaaca
    24961 atagatagag attctaagga cggaatgact ccttgagttt ttaaccaatt tgctgcagta
    25021 gtcaattgaa aatttgtgcc tttgggatct tggtaattaa gcaaaccaac aagatttggg
    25081 ttattactag ttgattgaat aaacgggaca acgaatttat tacattcttc aaaagaacta
    25141 accagaccaa aatcaatgat atgttcggtt acttcattgg tagagatggt gatggactca
    25201 aagacattgg gagaaataaa tgtgccactt ccaatattcg tatacagcaa acctttctca
    25261 attccatatt tataagcctt gccaacggta gtaaaattta aatctaaata atcagctagc
    25321 tctctttggg agggtaactg agtgtttttc tgtaatttat tctttaaaat atcttgttct
    25381 aattgttgaa tcaacgatcg ataataagga cgcttcaatt tttgtttgtc tggtcgccaa
    25441 gttaacgggt aattgtcata agaattaata ggcattttct ccactcctta aaattgtata
    25501 ccatacaatt ttaccattga ttgtattgat tttagaagtt aatcttttgt gaggagggaa
    25561 ttgtctatga aaattattaa ttttgaggaa gtagtagctt cttttagttt tgaagaagcg
    25621 attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct
    25681 cgattggtgg aaattttacc tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct
    25741 tatttggggg agaacagata ttttggcaca aaagtcatta ccgcttttcc ggataatcat
    25801 cagtatcatt taccttctca tttaggagag attttcttgt ttgatgctca aaacggacta
    25861 cccaaagcat tgataaacgc aaatgcagtc acctggattc gaacagctgc tgtatctgcg
    25921 ctggcaacag attacttagc aaatccgcat gcagaaacac ttgctttaat tggagctggt
    25981 caacaagcat tgtcgcattt gactgcgatt tctaccattc gtcccatcac tacagtgttt
    26041 gtttacgata ttttagaaga aagacgaaat acttttatcg ctaaagctaa aaaacagtat
    26101 cctaaaataa cgtttataaa tgcaatatca gtagaagatg cagtacgtcc agcggaaatc
    26161 atttgtacgt tgacctctag taaagaggca tttcttcaaa aagagtgggt aagggaagac
    26221 gcacatatca atgctgtggg cacatttact cctgatacaa gagagataag tacatcgttg
    26281 atggaaactt ctcaaatcta tgtagatgat tactcagctg ccttaacaga aagcggggac
    26341 ctgcttatag ccatggcaga aggagattta gaggaatctt gtattgttgg tacattaggc
    26401 gaattagttt ctgagaaaaa aacattttat aaaggagaat cgaggccgac gattttcgac
    26461 gcagttggtc tagctattga agatctttgt tgtgcagaat atatttataa caaattagag
    26521 aaaggggctt taaaatgaac ttagattatt acgtagttga tgcatttgca gatgaggtgt
    26581 tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc
    26641 aaaaaattgc cattgaaaac aatttatctg aaacagcatt tacagtgaag aaaaaccaag
    26701 aatttgagct tagatggttt acgccagatc gagaaatcga tctttgtggt cacgcgacat
    26761 tagcaacagc atttgtttta tttaactatt acaaaatacc tgatgaaacg attaaatttt
    26821 ctactcaaag cggtaatcta tttgtgacaa gaattaaaga ttattattac atggattttc
    26881 ctagtattat gccgaaaaaa gtgccgatat tgtctgaata tgaagaagcg attggggcta
    26941 agattaaaga agcctactta gcacgtgatt tatttttcgt attagaagat gaaaaaacgg
    27001 tggctaaatt gcagccagat tttacagcta tcaaaaattt tgaattagga ataggagtca
    27061 tcgtgacagc agaaagtgtg caaaaagact ttgtttctag aaccttcttt ccaaaattaa
    27121 caatcaatga agatccagta tgtggatcag cacattctaa tcttattcct tattgggcag
    27181 aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc atcaaggggc ggggatctag
    27241 aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa agcagttttg ttttctaaag
    27301 gtacctcgtt tatttgattt ctttcaaatg aattaaaaat aaattggtgg agagtggcaa
    27361 ccttatagct acaatggttg ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt
    27421 tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat
    27481 actaactatc ctcggatgac ttcgatttta taaccatcag ggtcaatgac aaagtagtaa
    27541 gaaggcgcag tgccaggtaa tccttttaag tcagtaatct caagaccagc agctttatgt
    27601 tgctcgtgta atttttctag atcatcagct gctatagcga tatgaccata gccgtctcct
    27661 agatcatacg caccatgatc atagttgtaa gtcaactcta attcatagtc atccccagga
    27721 agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat cacggcggcg gctttcttca
    27781 aaaccaaatg ccttttgata aaattcgatt gatgcttcaa gatctttcac tcgaacacat
    27841 gtgtgagcca ttttcataga ttcatctctc cttttccttt tactcaatca taacaaaaag
    27901 aagtaaggaa atagttgatt tgtgcttgtt catgagaaat ttctcatgta aaatggatag
    27961 aaaataaaac tgttaaggtg gaaactattc atggataaac caatttttgg tgaaaaaaag
    28021 caatttgctg attatcaaac ccgttatgca gcatatattg tcatagaaag agatcagcag
    28081 attgcagtaa tcgaggctcc aaatggtgct ttttttcttc ctggcgggga aattgaagga
    28141 gaagagacga aagaagaagc gattgtccgt gagctgatag aagaaatggg aattgtggca
    28201 gaaatttctt cttatctggg agaagctgat gagtattttt actcgaatta tcgtgcaact
    28261 tattattata atccaggata tttctttgtt gctgattctt ggaagcgtat aggagaacca
    28321 actgaaaaga ccaataaaat ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa
    28381 cgtggtagcc accgatgggc agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa
    28441 gcgagtagat cagctttctc tcagtttttt tatttattac agtacatatt ttttgattgc
    28501 ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca tttgctgctt ctttgacaag
    28561 agaaacggca tttcccattg ctactcctag accagcatat tcaatcatgg ataagtcgtt
    28621 ctcgttatct ccgatagcca tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt
    28681 cgcaactgcc gcacctttac ttgcttcttt atttagtact tcgaagtaat agggcgcact
    28741 tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa
    28801 aatttctggt tcgtcgatca tcatcatttt gacgatgttc atgtctggtg tcatttcttc
    28861 aggtgttcga tattttagcg gcatcttgac taaacttgct tcgtggatcg tataagcgct
    28921 gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc gaatggagat gactgccgac
    28981 acggcgtgcc atcacttcga tctctaggaa atcttcatgt gtcagtccgt gacgggcgat
    29041 gattttacct gatttcgttt gttgtacgag tgccccgtta taagtaataa cgtaatcatt
    29101 atcttgaaaa aggttcaact cgtttaactg ttcctggaca cctggtaagg gacggccagt
    29161 gcataaaacg atcgaaacac cttttgcaac agcagcggca atcgcttctt ttacttcttt
    29221 tgttacttct cttttctcat ttaataaagt accatcgatg tcgatagcga ctagtttgat
    29281 agacataatt tcctccaata tattttaatt tatttcaatc aatgcgccat tacggatatg
    29341 gctggcaaat tcttgataag tttcgtcaaa taaatcataa tgatcacgca aggacggatc
    29401 aaccatttct tttggaaaga aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc
    29461 aacaagctga ctaacctgtg ataattctat caaagttccg tcatttcgca aaatctcgat
    29521 ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca taaggtagat cgtagctaga
    29581 attcacagca gtgtaataaa caggattata tccgaccttt tctactaaca aagtgagttc
    29641 ttggactaat tttgaatccc ggttgccaga aaaagtagca gatttcagtg gtttacgatt
    29701 caaaaaacgt ttagctaaat catttaaaat aggatcagca gattcagacc attgagtaaa
    29761 gtaagtgccc aagaccccat catcaagttt gaggtattct tctaaagtaa agcttccgtc
    29821 aagaaatggc agaagtaatt gagaatggag ttcaaaactg ctggcatctt cttgatacaa
    29881 ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc ataccacggg aaacaggatg
    29941 gaagtacact tgcacataca tttgatatcg gctaacgata taatcttcca ctgcatgcat
    30001 gccactcatg gaaaaagcga tcccgccttc ataaggacga atcacacgta atattcgggt
    30061 aagatcaaat gttccatatt ctgttccagt gaaataagca tcccgaagca aatagtccat
    30121 tcgatctgca tcgatctgac tagaaatcat ttggacaact tgtgggttgg ggtaagtttt
    30181 ttggatcaca ctagctacct tttcaggaaa accttcttct actcggttaa gaatctgata
    30241 cacttctgtt tctggagaag tgatgatctc tacagtgatg gcttcatgat tcgtatggaa
    30301 gatatgttca aatgtatgag agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca
    30361 taaagtcacc agccgttcct gatcatccca gcctccgttg cctatttttt cttttgaaaa
    30421 atttcgagaa aagatatcgc agattcgtct tgaaatctca taaacgccta atgaatgagt
    30481 aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg
    30541 tcgtagacgt tggacttctt tcgaattgat caaatctaag attacttgat gctggacatg
    30601 gatatagtta tggactggat cacgaaaaac tttttccata ggcagcattt gatatttata
    30661 aggggttgtc ataatgtagc tcctttatgt tattacgttg ttccattctc tggatttggt
    30721 tgttccattg ctccagctgg atttcttttt ttagccagtc ggtagaatcc tgtaaatcac
    30781 ttgcagaata cagcttttca aaagcttttg caaatgcttc tttgacttct tttacaaaaa
    30841 ggggttggtc aagcagttct tcaagtgttg ccattgtagc aggatccact gcaggatagc
    30901 catttgtacc aaagtcttct tttaaagcag attgataaaa tcgcttcatg acttgcccgc
    30961 gtttttcctg attaccgttg acacttaagt acatcataac agaaatccca tttttgatcc
    31021 gacgctgagc gataccggca aattttttgc catggatgct taaatcataa gttccaggac
    31081 aataagaatg ggtgatttcg aatgcttgta tcggtgcctt tggaaaagcg tatttagtca
    31141 ataggagcat ttgctcgtaa gcgtcatcta tggttatttt ttttgtgtca ctttggggga
    31201 aaatcaaaga aatattcaaa attccttcat ctgatacgat ccctaatcca cctgaatttc
    31261 tgatcactgg agcgtaacct gcatctttca gtgtttggat cccgtcactt agaaaaggag
    31321 ttcgaacatc cttcatacct aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga
    31381 tttgctggtg ttctttagaa tattccgtta atatatctgt taatgcaaaa ggggaaaagt
    31441 cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg gttcattcaa ccggctcctt
    31501 tgtataaatt catattttct acaatattat actctaaaat caaagagttg ttttggaaaa
    31561 aaggttttct gtaaaaatgg acaagttggt tgacaaaaca aaagaaacta ggaacaatgt
    31621 agctatgaga agtaaaggag aatgaataat ggaattatca caaggaacac cgatttcaat
    31681 caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa
    31741 agggcaaatg gtgacaattg gcgatacgct gtacatacgt tataaagaga ttcaggagga
    31801 gacgggcgat gagattcctg taacgatcaa attagttcca gatggtcatg tacagctgat
    31861 ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat aaagaacggt tagaaacatc
    31921 ctatcgaaca ccatacggta tgatccagat tgctactttt acgaaagaac tgcatgttag
    31981 tttgaaagac cgtcccacag cagggaaggt tcgaatcgat tatgatctgt tcatgggacc
    32041 ggaaaaaatc ggcgaatatt atttgacctt ggattttaca gcgtaaatat aaaagaaaag
    32101 tttgattttt tgtgtctgat tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg
    32161 attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc
    32221 acacgcgatc ttagaacaac gtggcgatgt catggatttt tctgatttag ctaaccagat
    32281 ccaaaactat cttgggaaat cagacagtga aattcgtgat tcactcgcac aattttatac
    32341 tgacttgaat attgacggca gctttatctc attaggggat aatcgttggg gcttgcgttc
    32401 atggtatgct atcgattcga tcgatgaaga agtaaaccat ggaatggacg aagaggatga
    32461 agatactcca cgtcgccgta aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga
    32521 cgcgatcgat tacaatgacg atgacccaga agatacagat ctgacagaag aagatgacga
    32581 tgatctgttt gatgacgatg atgatgaaga tgaagaaatc gctgcttata attctgactt
    32641 gcaagaaatc ggagcagatg acccgacaga tgatgaagaa gacttgccag gaaatatcga
    32701 agaagattta agtatcatag acgaagacga tgatgatgaa gattatgaag aggaagaatt
    32761 ttctgactct gaaaaagaat aaacattatt ttcttacgag aggttgtgac agagacgatc
    32821 agcctatcag aataaggagg tgtgacacga tttttgtcac accttctttt ttgtatataa
    32881 aaatcccaag taaatacata aaaaataaaa aaattgaaac ttttagcgca ccttgtacgt
    32941 catattaagt gaggcagaaa ggagtggaga atgattttta ctggatataa gaaaaaacgg
    33001 cagatcgaaa aataccgtca cctgctgaat ggactcatcg aaactgatta tcaaaaaatg
    33061 ttccgtatca cgatgaatta cgtacatgat aaagaagagg ccttagatgt gatgcaagat
    33121 agttttcata aagccctgaa ctattttgaa aaagaaaaag agatcgagca ttttagtgca
    33181 tggttttatc ggatattgat ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg
    33241 cctgtggact tatttgaaat tcaagatatc ccccaacagt tacctttaga aacttccgtt
    33301 gcagagctcc atatgatttt agataaaata gacagtccgg aaaaagagat tctgatcctc
    33361 tacttttttg aaggatttcg tttgaaagaa attgcgacta tcttggatat gaatgaaaac
    33421 acagttaaaa cgaaaatgta ccgctcattg aatgctttac gtcaaatcct agaatgaagg
    33481 agagaagagt gccatgtatt cccaaaaaga aaatttaaaa aaactaaaga aaacttataa
    33541 agaacaagca attcccaaag ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga
    33601 gcaatcattt ataaaagcaa agcggagaaa gagacgtctc caagggttcg gagtatcttt
    33661 ggctatcgta atgattacaa caagtagcct tctttttaat agtcaagttc gaagctttgc
    33721 agaagattta cccatcttag gatcagtcat tgaattgatc ttaggcgaac gctttactga
    33781 tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa
    33841 taaaacaatt catggattaa atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa
    33901 agctgaaaaa gaatatggaa attttgaaac ggatcattac caagttttgg gagattatca
    33961 gaaaatcgta gacgataacc ggtttctcgt gattgaaagg cagatcaccc aaacagcagc
    34021 agatagccat gttgaaaaaa gatacgacac aatcgacaag aaaaacagtg tccagctctc
    34081 acttccactt ttattcaaag atgacaccta tctatctgtt ttgacaaaag aagtcaaacg
    34141 gcagatggcc gaacaagtca aggaagatcc atctaagtac tattggactg agcaagatat
    34201 tcaagaaggg acgatagaaa aaccaacact tgttacgcct acacgaagtt tttatttgaa
    34261 caaagagcat caattagttt tgactttttc tcaatacgaa attgctcctg gttatatggg
    34321 tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg attttggcat cggaagatta
    34381 tttggatcat tagacgagaa gtagttgcga ctagcagttt caattttcaa gtaagtacaa
    34441 gtaaataata agaagagaaa aagagaaaga agagatatag tgaaaagaaa ttatttagtt
    34501 atcagtatta ttgctttatc cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg
    34561 atcaccgaag taaaaggaca aacacactct tcggagcatt cgtcacaatc tttcaaacaa
    34621 tcagaacaat caaccattaa aagccaatca actgaaacag ctaacgtcaa agaagccatt
    34681 caagaatcag cggaaaaggt acaaagtcag tcatcacaat caacacaaga cagtacaaaa
    34741 acgagccgag atccggctgt caaaagcgtc gcaatcagct ttgatgatgg accaggagcg
    34801 accacaacac cgcagcttct gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc
    34861 gtcttaggag aaaatacggc gcagcatcct gaaatcgtga agcagacagc tgaagcaggg
    34921 cacgagattg gcaatcacac atacgatcat cagaatctgg ccactctttc tgcccagtcg
    34981 atgacggagg aagtgacgaa agcagacact gagatcaaaa aagtcactgg caaaaccccc
    35041 acttttgttc gtccgcccta tggttctgtc acgaatgtag gagcaacgat catccagcga
    35101 ccgatcatcg aatggtctgt tgattcagaa gattggaaga caagaaatcc tgatttgatt
    35161 ttgcagaaga tccaagcaac agtatacgat ggggcaatta ttttattcca tgatatctat
    35221 cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc tgaaagagca agggtatcgc
    35281 atcacgacag ttggggattt attagggcat ccaacggctg tcgagaacta ttatggcaga
    35341 aacgatcatc ggccagttca gtaagtgaga caagtatctt taataaataa cacattttaa
    35401 taagtagttg aacacataac taccttactt gcaaaggctt aacaatatct atacaggtac
    35461 aacaaaaaac tgtccaccaa ttttctttgg cggacagttt tcacttaaaa ataaagaagc
    35521 aactattcgt aaattatgct aaaatagcag tagaacgcgt cctgagggaa tcgaaccccc
    35581 gtctcaagaa ccggaatctt acgtgatatc cactacacta aggacgcaag cactgggata
    35641 attgtaacta aacagataaa aaattacaag gggattggac aaaaaaatga aatttgagca
    35701 aaatttttca caaaaacagc agcagacaca gaaactagcc atgacgcaac agttgcaaca
    35761 gtccatccaa gttttacaat ataacagtga ggagctgttg gcattcgttg agaatcaagc
    35821 aatggaaaat ccattggtgg aagttgttga gccggaatgg cagccagatt acataaaagc
    35881 ttcttcatct tcttatgaag gagaagagac aaattaccta aatcagattc ctgatacaaa
    35941 agggtcttta tttgattcat tgatcgaaca agtccatttg aattaccgag atactttttt
    36001 gaggaaaatc gttctttatc ttgtagaata catcgatttg aacggatttt tgacgattag
    36061 tttagaagaa gccggaaaag agattggagc tacgcctatc caaatgctcg atgcgttgac
    36121 tctgattcag caattagaac cagctggagt aggtgcacgt agtttgcaag aatgtctcat
    36181 gcttcaaaca gaacgagatg attatgcgcc ggaattagct tatatcgtgt tggaagaatg
    36241 ttttgaggaa ctggtggagc gaaaatggaa agaaatcgca caacgttttt ctgttgatct
    36301 acatgctgtt cagcagatat tcgactattt acaaacgttg agtccaagcc ctggccggat
    36361 tttcgaccgt agcagcgaat tgtacattat tcctgatgtc cgggtattag tggacgacga
    36421 taaaaatgta caagttatct caaaccgaaa aaatcagcca aatatacgct ttcaagagag
    36481 ttacttcaaa caaatgtcac aacaagcaga taaagaaact gaaaattatt taaaagaacg
    36541 gaaacaggaa ttcgaatggt tgaagaaaac gatcctacaa cgtggggata caattttgcg
    36601 ggttgctcaa gtcattgttt cccggcaaaa agctttcttt atcgacaagg aacgtccaat
    36661 caagcctctt acgttaaagg aagttgcaac tgagatagat gtgcatgaat ccacggtcag
    36721 tcgtgcagtc aacgggaaat atttggaaac agacttcggt gtatttgaat taaaaaaatt
    36781 tttcactaca cgaattccta cgaatagtac agaacagaca gaagaccttt cagcagatac
    36841 agcgaagaaa aagctgcagg aattagtcga tcaagaagac aaaaacaagc cgttgtctga
    36901 ccagaaattg gttgaattgc tgaaaaaaga tgagatagcg atctcaagac gaacagtagc
    36961 aaaatatcgt gatttattag gaatcccgag ttcatcaaaa agaaaacgtt atgataacta
    37021 atcagtcaaa agactagaaa aagtatttca ctttttctag tctttttagt tgaaaaacga
    37081 gagcaaaact gttagactta cagtcgtgag gttgagattt ccctttattg atgtgaaatt
    37141 cgaatagtta atttcttttt ttatcgtata tgggtcgttt taagtctaca ttggacgtaa
    37201 agtgtccaac taaggagatc gctatgctgg atgaaataaa aatgattgaa tctgttgcac
    37261 cagatattat cgaagtgttg caagaacgct ataaaatttt acgaaatatc tattggatgc
    37321 agccgattgg acgtcgaagt ttatcggaaa gtatggggat cacagaacga gtattgcgaa
    37381 cagaaacaga tcttttgaaa aatctgaatt tgatcgacac ctcaaaaagc ggtatgacgc
    37441 tgacaactaa gggcgaagaa gtttatcaaa gccttgaaaa cttcatggat cagcttttag
    37501 gtacgcatca aaccgaacaa cagttggcag aatattttgg taaccaacgc tgtatcgttg
    37561 tttcaggaaa cagcgaagag cagaccaaag tggctgatgc attcggcgaa gcgctgagcg
    37621 aagccctaga ccgactactt ccagaaggcg agaatatcat cgctgtgatg ggaggaacta
    37681 ctatggctgt ggtagccgaa caattgtcta atctggaaaa taagaaacgg cataacctgt
    37741 ttgtccctgc aagaggcggg attggtgaag cgataactgt acaggcaaat tctgtcagtg
    37801 caagaatggc tgctaaagca aacggtaacc accgtgcact ttatgttccg gaacaattaa
    37861 gtctggcaac ttacaattct ttactcaatg agccatctat ccaagaagtc ttaaacttga
    37921 ttagtgaagc gaattgtgtt attcatagta ttgggcgtgc attacacatg gcagcacgtc
    37981 gtaagatgac tgaaaaagaa ttagtcatgc tgaaacaagc aaatgctgtt gcggaatctt
    38041 ttggctactt tttcaatgaa aaaggtgaag tcgtttataa ggtccctaga atcggcatag
    38101 aattgcaaga tttggaaaaa gtcccgatca tcatggcaat cgcgggcgga aaatcaaaag
    38161 ctaaagcaat tcgagcttat atgaaaaatg caccaaaaca aacgtggctc atcactgatg
    38221 aagctgctgc aaatgagatt ttaaaagggg taacccttta aaataaatat ttttttgatt
    38281 ttcataagga ggaaatctta aatgacagtt aaagtaggta ttaatggttt tggacgtatt
    38341 ggacgtctag cattccgtcg tatccaagat gtagatggaa tcgaagtagt agcaatcaac
    38401 gacttgacag atgctaaaat gttggcacac ttgttaaaat acgacacaac tcaaggacgt
    38461 ttcaacggaa cagttgaagt tcatgaaggt tcattcaacg taaacggaaa agaagttaaa
    38521 gtattagcta accgcaaccc agaagaatta ccatggggcg aactaggcgt agatatcgtt
    38581 ctagaatgta ctggtttctt cacttctaaa tcagcagctg aaaaacattt aacagctggt
    38641 gctaaacgcg ttgttatctc tgctcctggc ggaaacgatg ttccaacaat cgtttataac
    38701 acaaaccacg aaacattaac tggtaaagaa acagttatct caggtgcttc atgtactact
    38761 aactgtttag ctcctatggc taaaacattg aacgacaaat ttggtgttgt tgaaggatta
    38821 atgacaacta tccacgctta cacaggtgac caaatgactc tagacggacc tcatcctaaa
    38881 ggtgacttcc gccgtgcacg cgctgctgct gcaaacatcg ttcctaactc aacaggtgct
    38941 gctaaagcta tcggtttagt aattcctgaa ttgaacggta aattagacgg agctgctcaa
    39001 cgtgttcctg taccaacagg ttcattaaca gaattagtaa cagttcttga aaaagaagta
    39061 actgttgacg aaatcaatgc agcaatgaaa gaagcttcaa acgaatctta cggatacaac
    39121 acagacgaaa tcgtttcttc agatatcgtt ggtatgactt acggttcatt atttgatgct
    39181 acacaaacta aagtaatgac agttggcgac aaacaattag ttaaaactgt tgcttggtac
    39241 gacaacgaaa tgtcatacac tgcacaatta gtacgtactt tagaatactt cgctaactta
    39301 taagatagac aaacgtacaa gtgtaaatta tagacatgta catttaaaag cggggaagca
    39361 atcgcgcttc ttccgctttt tttgataata agtttattca ggaggaaata aaatggctaa
    39421 gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt cttgtccgtg ttgacttcaa
    39481 cgttcctttg aaagacggcg tgatcactga cgacactcgt atcaaagcag cattgccaac
    39541 aatcaactac gttctagaac aaggcggaaa agcaatcctt ttctctcacc ttggacgtgt
    39601 aaaaacagaa gaagataaag aaggaaaatc tttagctcca gttgcaaaac gtctaggcga
    39661 attattaggt aaagaagtaa cattcgtacc tgaaactcgc ggagaacaat tagaagaagc
    39721 aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa aatacacgat ttgaagatat
    39781 tgacggtaaa aaagaaagca aaaatgatac agaactaggt aaatactggg cttcattagg
    39841 cgatgtattt gttaacgatg ctttcggtac agctcaccgt gcacatgctt ctaacgtagg
    39901 tatcgcttca actggtatcc caacagttgc tggattcttg atggaaaaag aaatcaagtt
    39961 catcggtgaa gcagtagaag aaccaaaacg accaatgatt gcaatccttg gtggcgcaaa
    40021 agtttctgat aaaatcggcg taatcgaaaa cttgattcct aaagcagata aaatcttgat
    40081 cggtggcgga atgacttaca cattctacga agcaaaagga atcaagatcg gtaactctct
    40141 agtagaagct gataaagtgg aactagcaaa agaattaatc gaaaaagcag gcgacaaact
    40201 tgtcttgcca atcgacaatg tctgtgcacc agaattttca aatgatgttg aaacacaagt
    40261 aatcgaagga gacattcctg atggtttgat ggctttagac atcggcccag catcagtaaa
    40321 attatttgct gatacattac aaggtgcaaa aacagttgtt tggaacggac ctatgggtgt
    40381 atttgaaatg agtaactttg ctaaaggtac aatcggtgtt tgtgaagcaa tcgctaactt
    40441 agaaggcgca acaacaatca tcggtggcgg agactcagcc gcagcagcag aacaattagg
    40501 atttgctgac aaattcacac acatctcaac aggtggcggc gcaagcttgg aattgcttga
    40561 aggtaaagaa ttaccaggac tagcagcaat caacgataaa taaacgacca agtttgctca
    40621 attctgacga gtaatcgaac aaaaggtgaa gggagttttc atgactccct agccattttt
    40681 caataaatta aggagtgtgt tcctcaatgc gtaaacctat tatcgcaggt aactggaaaa
    40741 tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc agtaaaaaac aaaatccctt
    40801 caaatgacaa agttgattca gtcatcggat ctcctgcatt attcttacaa gaattaatga
    40861 catcagctga aggtacagaa ctaaaaatct cagcacaaaa ctgctactgg gaaaactcag
    40921 gtgcgttcac tggtgaaact tcaccagcag ctcttgctga tctaggtgtt cattatgtga
    40981 tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac agacgaagac atcaataaaa
    41041 aagcgaaagc tatctttgca aatggtatga caccaatcct atgctgtggc gaatctttag
    41101 aaacctatga agcaggcaaa actgctgaat ggatcgaagg acaaattaca gctggtttag
    41161 ctgatctttc agctgaacag gtgtcaaaca tggttattgc ttatgaacca atctgggcaa
    41221 tcggtactgg taaatcagct gacgcacaaa tcgctgacga aatttgtgga gttgtacgtc
    41281 aaacagttga aaaactttat ggaaaagaag tatcagaagc agttcgtatc caatacggcg
    41341 gttctgtaaa accagaaaat attgctgaat acatggcaaa agaaaatgtt gacggtgctt
    41401 tagtcggtgg cgcaagcttg caagcagatt cattcttgtc attgttagac gctgtaaaat
    41461 aattgttgaa aatttgcgaa aaatgcaaga aatgtttgca ctaatgagca aaattaacta
    41521 aaatcgtata tgagggaaat cccttaggaa aaaacaaact caaaggagag acaaaacatg
    41581 tcaattatta ctgatgttta cgctcgcgag atcttagact cacgtggtaa cccaacaatc
    41641 gaagtagaag tatacacaga aagcggagct tttggccgcg gaatggttcc ttcaggtgct
    41701 tcaactggtg aatacgaagc agttgaatta cgtgacggag acaaagctcg ttatggcggt
    41761 aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca ttgctgaagc aatcattggc
    41821 tacgatgttc gtgaccaaat ggctatcgac aaagctatga tcgctttaga cggaactcct
    41881 aacaaaggta aattaggtgc gaacgctatt ttaggtgttt ctatcgctgt tgcacgtgca
    41941 gctgctgact acctagaagt acctttatac cactacctag gcggattcaa cacaaaagta
    42001 ttgccaacac caatgatgaa catcatcaac ggcggatcac atgctgataa ctcaatcgac
    42061 ttccaagaat tcatgatcat gcctgttggc gctccaacat tcaaagaagc tctacgtatg
    42121 ggtgctgaag tattccacgc attagcttca atcttgaaag ctcgcggatt agcaacttct
    42181 gttggtgacg aaggtggatt tgctccaaac cttggttcaa acgaagaagg ttttgaagta
    42241 atcatcgaag caatcgaaaa agctggctat gtacctggta aagacgttgt tcttgctatg
    42301 gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg tttacgtact agctgattca
    42361 ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct acgaagaatt agtttctaaa
    42421 tatccaatca tctcaatcga agacggatta gacgaaaacg actgggacgg attcaagaaa
    42481 ttaactgacg tattaggcga caaagttcaa ttagttggtg acgacttgtt cgtaacaaac
    42541 actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta actcaatcct tatcaaagtg
    42601 aaccaaatcg gtacattaac agaaactttc gaagctatcg aaatggctaa agaagctggc
    42661 tacacagcag ttgtatctca ccgttctggt gaaacagaag attcaacaat ctctgatatc
    42721 gctgttgcaa caaacgctgg ccaaatcaaa actggttctc tatcacgtac tgaccgtatt
    42781 gctaaataca accaattact acgtatcgaa gaccaacttg gtgaagttgc agaatacaaa
    42841 ggtttgaaat ctttctacaa cttaaaaaac aaataattct tttctaatga agatacatga
    42901 tcgactattg aaagatagtc gagtatgatc acttagttaa gtaatcgaac tctctacttg
    42961 ctgaaatatc agcaggtaga gagttttttt gcaactagct tctgtatatg atgtttacaa
    43021 agaatggtac aagaaaagat catccttgaa aaaggaaagc taacaataac tagcatcata
    43081 taagagcttt taaggataga acagtagaat aggaatctat gagcaagtcc tctcgtataa
    43141 ataaaatagg agggaagtag cagcatattt ttcataaagt ggctctcttt cttcattttt
    43201 actttggttg ctcctatttg aaaattcgag cacactcaaa tacaataaaa agaaatggca
    43261 agggagagga ttagatgaaa aaagaagaaa aaatcgcgat tttacaagag atcatccgaa
    43321 ttaaatcagt gaatggaaac gaaggtgaag tagcagccta tttgaataag cttttggcaa
    43381 agcatgacat aacaggagag attgtttctt acagagatgg tagagataat ttgattgctc
    43441 gttaccaaaa gggccaatca ggaaaagtgt taggcctatc tgggcatatg gatgtcgtgg
    43501 cggctggtga tgaatcgtct tggacctatg caccctttgc agcagaaatc catgggaacc
    43561 gtttgtacgg acgtggtgct acagatatga agagtggctt ggcagcaatg gtgattgcca
    43621 tgatcgaact gaaagagtca ggaaaaccat ttaacggaac agtcaaatta ttagccactg
    43681 tcggagaaga agtgggtgaa ctaggtgggg aacagctcac gaaagcaggc tatgtagatg
    43741 acttagatgc cttgatcatc ggagagccaa ccaattacag tttgatgtat acgcacatgg
    43801 gatcaatcaa ctatacagtc acctcgcacg gaaaagaagc gcatagctct atgccagacc
    43861 aaggttataa tgcaatcaat cacctaaatg aattcattac gaaagcaaat gctgaaatga
    43921 atcacctcgc tgaaacaatc gagaatcctg tcctaggaaa aacaatccat aatgttacat
    43981 tgatcagtgg cgggaatcaa gtcaacagca tcccaagcca tgcgcagctc caagggaaca
    44041 ttcgatcgat tccagaatac ccaaatgata aaatcattgc attgctgcaa tcaatcgtca
    44101 atgaattgaa tcaagagaca gactatcatt tggaattgat gatcgattac aacaaaatac
    44161 ctgtcaaagc tgatccagat tctcctttga ttcattgcat tcagcaacaa ttcagccagc
    44221 cattaccttt agttggagcg gcagcaacaa cggatgcagc cgaatttacg aaagctaacc
    44281 attccttcga ctttgtcgtc tttgggcctg gtgtcgttac tttgcctcat caagtagatg
    44341 agtatgttga aatcgataat tatttggata tgatcgaaaa atatcaagga attattttat
    44401 cctacttagc gtaagatttt tttaataaat gaaaaaacag agccagatta gcattattt
//
LOCUS       DAYYAO010000014        43386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000014 DAYYAO010000000
VERSION     DAYYAO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.015"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..1163)
                     /locus_tag="ACHARI_000683"
     CDS             complement(60..1163)
                     /locus_tag="ACHARI_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8441756.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1385..1855)
                     /locus_tag="ACHARI_000684"
     CDS             complement(1385..1855)
                     /locus_tag="ACHARI_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8441757.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2188..3828
                     /locus_tag="ACHARI_000685"
     CDS             2188..3828
                     /locus_tag="ACHARI_000685"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8441758.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3982..5304
                     /locus_tag="ACHARI_000686"
     CDS             3982..5304
                     /locus_tag="ACHARI_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8441759.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5366..5797)
                     /locus_tag="ACHARI_000687"
     CDS             complement(5366..5797)
                     /locus_tag="ACHARI_000687"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8441760.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5861..6697)
                     /locus_tag="ACHARI_000688"
     CDS             complement(5861..6697)
                     /locus_tag="ACHARI_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8441761.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(7439..8065)
                     /locus_tag="ACHARI_000689"
     CDS             complement(7439..8065)
                     /locus_tag="ACHARI_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441762.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8360..8695)
                     /locus_tag="ACHARI_000690"
     CDS             complement(8360..8695)
                     /locus_tag="ACHARI_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8441763.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(8682..8966)
                     /locus_tag="ACHARI_000691"
     CDS             complement(8682..8966)
                     /locus_tag="ACHARI_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8441764.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9022..10545)
                     /locus_tag="ACHARI_000692"
     CDS             complement(9022..10545)
                     /locus_tag="ACHARI_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8441765.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10538..11713)
                     /locus_tag="ACHARI_000693"
     CDS             complement(10538..11713)
                     /locus_tag="ACHARI_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8441766.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(11717..11902)
                     /locus_tag="ACHARI_000694"
     CDS             complement(11717..11902)
                     /locus_tag="ACHARI_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441767.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(11868..13562)
                     /locus_tag="ACHARI_000695"
     CDS             complement(11868..13562)
                     /locus_tag="ACHARI_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8441768.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13559..14032)
                     /locus_tag="ACHARI_000696"
     CDS             complement(13559..14032)
                     /locus_tag="ACHARI_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8441769.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14101..14256)
                     /locus_tag="ACHARI_000697"
     CDS             complement(14101..14256)
                     /locus_tag="ACHARI_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8441770.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14390..14770)
                     /locus_tag="ACHARI_000698"
     CDS             complement(14390..14770)
                     /locus_tag="ACHARI_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8441771.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(14774..14989)
                     /locus_tag="ACHARI_000699"
     CDS             complement(14774..14989)
                     /locus_tag="ACHARI_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441772.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(14992..15399)
                     /locus_tag="ACHARI_000700"
     CDS             complement(14992..15399)
                     /locus_tag="ACHARI_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8441773.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(15665..17140)
                     /locus_tag="ACHARI_000701"
     CDS             complement(15665..17140)
                     /locus_tag="ACHARI_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8441774.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17130..17978)
                     /locus_tag="ACHARI_000702"
     CDS             complement(17130..17978)
                     /locus_tag="ACHARI_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8441775.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18015..18377)
                     /locus_tag="ACHARI_000703"
     CDS             complement(18015..18377)
                     /locus_tag="ACHARI_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441776.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18378..18536)
                     /locus_tag="ACHARI_000704"
     CDS             complement(18378..18536)
                     /locus_tag="ACHARI_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441777.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(18677..19301)
                     /locus_tag="ACHARI_000705"
                     /pseudo
     CDS             complement(18677..19301)
                     /locus_tag="ACHARI_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19298..19609)
                     /locus_tag="ACHARI_000706"
     CDS             complement(19298..19609)
                     /locus_tag="ACHARI_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441778.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(19653..19946)
                     /locus_tag="ACHARI_000707"
     CDS             complement(19653..19946)
                     /locus_tag="ACHARI_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441779.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20140..20787
                     /locus_tag="ACHARI_000708"
     CDS             20140..20787
                     /locus_tag="ACHARI_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8441780.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            20848..21993
                     /locus_tag="ACHARI_000709"
     CDS             20848..21993
                     /locus_tag="ACHARI_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8441781.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22074..22379)
                     /locus_tag="ACHARI_000710"
     CDS             complement(22074..22379)
                     /locus_tag="ACHARI_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8441782.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22404..22835)
                     /gene="ruvX"
                     /locus_tag="ACHARI_000711"
     CDS             complement(22404..22835)
                     /gene="ruvX"
                     /locus_tag="ACHARI_000711"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8441783.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(22832..23098)
                     /locus_tag="ACHARI_000712"
     CDS             complement(22832..23098)
                     /locus_tag="ACHARI_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8441784.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23353..24078)
                     /locus_tag="ACHARI_000713"
     CDS             complement(23353..24078)
                     /locus_tag="ACHARI_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8441785.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24439..24509)
                     /locus_tag="ACHARI_000714"
     tRNA            complement(24439..24509)
                     /locus_tag="ACHARI_000714"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24475..24477),aa:Gly,seq:ccc)
     gene            24626..25378
                     /locus_tag="ACHARI_000715"
     CDS             24626..25378
                     /locus_tag="ACHARI_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8441786.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25394..25873
                     /locus_tag="ACHARI_000716"
     CDS             25394..25873
                     /locus_tag="ACHARI_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8441787.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26124..27704)
                     /locus_tag="ACHARI_000717"
     CDS             complement(26124..27704)
                     /locus_tag="ACHARI_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8441788.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(27697..28440)
                     /locus_tag="ACHARI_000718"
     CDS             complement(27697..28440)
                     /locus_tag="ACHARI_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441789.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(28616..29392)
                     /locus_tag="ACHARI_000719"
     CDS             complement(28616..29392)
                     /locus_tag="ACHARI_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8441790.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(29640..30476)
                     /locus_tag="ACHARI_000720"
     CDS             complement(29640..30476)
                     /locus_tag="ACHARI_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8441791.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30487..31416)
                     /locus_tag="ACHARI_000721"
     CDS             complement(30487..31416)
                     /locus_tag="ACHARI_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8441792.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            31608..31961
                     /locus_tag="ACHARI_000722"
     CDS             31608..31961
                     /locus_tag="ACHARI_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8441793.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32035..32691)
                     /locus_tag="ACHARI_000723"
     CDS             complement(32035..32691)
                     /locus_tag="ACHARI_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8441794.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            32917..34851
                     /locus_tag="ACHARI_000724"
     CDS             32917..34851
                     /locus_tag="ACHARI_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8441795.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(34937..36160)
                     /locus_tag="ACHARI_000725"
     CDS             complement(34937..36160)
                     /locus_tag="ACHARI_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8441796.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36154..38025)
                     /locus_tag="ACHARI_000726"
     CDS             complement(36154..38025)
                     /locus_tag="ACHARI_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8441797.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(38046..39164)
                     /locus_tag="ACHARI_000727"
     CDS             complement(38046..39164)
                     /locus_tag="ACHARI_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8441798.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39247..40080)
                     /locus_tag="ACHARI_000728"
     CDS             complement(39247..40080)
                     /locus_tag="ACHARI_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8441799.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40073..40990)
                     /locus_tag="ACHARI_000729"
     CDS             complement(40073..40990)
                     /locus_tag="ACHARI_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8441800.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41217..42341)
                     /locus_tag="ACHARI_000730"
     CDS             complement(41217..42341)
                     /locus_tag="ACHARI_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8441801.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            42524..>43386
                     /locus_tag="ACHARI_000731"
     CDS             42524..>43386
                     /locus_tag="ACHARI_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8441802.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFK"
BASE COUNT        13068 a         8754 c         7173 g        14391 t
ORIGIN      
        1 tataaaaata ggagtacggc atcatctgcc gcactcctat ttttttcgtc atttgtttct
       61 taaataaaac gattctcacg caattttatt tttttgatat cttctactgt ttgtgttcca
      121 gctaattgca tgaccatttc caattctgtt ttaaagaaat caaacacttg atggactcct
      181 gtacttcctc ctaaagacaa tccataaata acgggtctgc caatagcaac aagatctgca
      241 cctgatgcga ttgctttgaa aacatgctgt ccgcgtcgta caccgctgtc aaacacgatt
      301 ggtacacgtt tatctactgc ttctgccaca tattgcaacg aatcaaacgc tgctggacca
      361 ccatctagtt gacggccacc atgattagat acccagattc cgccagcacc agattctaat
      421 gaacgataga catcttcttc tgactggaca cctttcacat agactggcaa atcagaatga
      481 gcagcaataa attctacatc ttttggacta agcttctgtt tggaagattt gtacacagca
      541 tccatcgttt ggcctactcc tgattgataa gcctgaacga tcggcattgg cagtgggaag
      601 gtaaacccat ttctgcggtc tgtttcacgg tttccgccaa ctgttgcatc cgctgtcaat
      661 acgattgctt ttgctccatt acgcttggcc acttcaagaa tatccaaatt gatcccatca
      721 tctttgctca tataaaattg aaaccattgc ggtgctttct ccccgcctgc ttctcttatc
      781 tcttctaatg tacaagaagc gtaagaactt gctgtataga tcgtcccaaa atctgctaca
      841 ccttttgcag aagctttctc cgccttcaca tgagctaagc catgagcagc aactggtgcc
      901 atgatgattg gtgcagtcag cgtttctttg tcaaaatgtg tagttgtatc gggtaattct
      961 acatctttta atacatgagg aataatcagc tgatgattga atgcccgttc attttcttga
     1021 taagtaaata agtcgccagc accactgctg atgtaaccat aaccaccagc tggaatcact
     1081 tgtgaagcgg ctatttctaa atcttccata ttgataaaat ccactattcc ttcatttgta
     1141 cctgcttgat acgttttttc catttcaatg ccttccctct ataaaaaatt gaatgaataa
     1201 tatgatagcg tttacacact ttatattact cttccgtatt gatacaggca aataatctgt
     1261 ttacatttca gtcattccag ttgttactac tctgaaagaa atgtttagaa cacaaataga
     1321 taaaaaacga tgggactttg acaaaagtcg tgtcaaagtc ccttttacga aatactgtgg
     1381 atgcctattg aaataagtgc gggttgtttc tttgtatttc aatcgtttct tgccattctt
     1441 cgtttaattt tggattaccg ttttcttcct tgaacgcata aggtcccaat ccaaaaagct
     1501 cagttagtat ttgattcacg attccatata gctcggtgtg atcggacaca tgattattga
     1561 agatcatctc attatcacca cgtttgaatc tcacattcgg gctctcatga tgaacctcta
     1621 tgcagttttc cccttttttt aactgacgga tttctgaaaa catctcataa atatcttctc
     1681 caataaaacc ttcagcaagc gccagactgt ccacaagacc gcctactgtc aactcagaga
     1741 ttatcgcttt gttggcacca agctttaatg tatagcttgt tgcttctact acgccgcatg
     1801 cattgcaacc accttctgat agttcgcttt ttctgatcaa ttgaacttct ttcattttct
     1861 gttcctcctc ttagttttct acttatgtat tttttatcac ctgacgtaag tgtattttat
     1921 cgtcacgatc acgtgattta ttcttcagag attctattta tctatctaac atatccttta
     1981 ttcttctact tttctttctt tagcatataa gagatacgca tagtaatcaa gcaaggattg
     2041 ttgatataaa aagaacaaat accgctattt tgatttcttt tgactctatt actcatataa
     2101 ataaaattta ttatacctgc tctttttgac tatatcctca tctgactcct atacttcgtt
     2161 tgtattaaaa cttaggagga atcattcatg cttacagggc taaacttatt aaataatcct
     2221 tttctaaata aaggaacagc ttttacaaaa gaagaacggg caaaatacgg aatcactggt
     2281 atgcttccaa gtactgtcca aacattggaa caacaatctg tccaagctta cggtcaatac
     2341 ttaagcaaac agaccgattt ggaaaaacgt atcttcttaa tgaatctatt caatacgaac
     2401 cggacattgt tttataaatt gatgggcgaa catttagttg aattcatgcc tgtcgtttat
     2461 gatcctgtcg ttgcggattc aatcgaacaa tacaacgaaa ttttcttgaa accacaagat
     2521 gctgcatttc tttcaatcga cgaaccagaa aatatcaaag caagtttgaa aaacgcagca
     2581 gatggacgcg acattcgttt gatcgttgtg acagatgcag aaggaatctt aggtatgggt
     2641 gactggggtg tgaacggtgt tgacatcgct atcggtaaat tgatggtcta cactgctgcg
     2701 gctggtatca atcctgcaca agtcttgcct gtatcaatcg acgctggtac taataacgaa
     2761 gaattattga ataatccatt atacttaggg aatcgtcatg ctcgtgtaga aggcgaaact
     2821 tactacgaat tcattgatca attcgttcaa tcagctactg aattattccc agaattgtta
     2881 ttacattggg aagattttgg acgaggaaat gctgcaacga tcttagaaaa atacgaagat
     2941 aaaatcacta ctttcaatga tgatatccaa ggcacaggaa tcgttgtact agctggtgta
     3001 ttaggtggat tgaatatatc aggtgagcaa ttgaaagatc aaacgatctt gacatttggt
     3061 gctggtactg ctggtgtagg tatcgcaaac atccttttag atgaaatgat ccgtcaaggt
     3121 gtacctgaag aagaagcacg ccaacatttc tatcaagtag ataaacaggg tttattgttt
     3181 gaagatacaa aaggattgac tcctggtcaa atcccattcg ctcgtaaacg ctcagaattc
     3241 actaatgctg atgaattgac aaacttagaa gcagttgtaa aagcaatcca tccaacgatc
     3301 atgatcggta cttctaccca acctggagca ttcactgaag aaatcgtgaa agaaatggcg
     3361 gctcataccc cacgtccaat catcatgcct ttgtctaatc caacaaaatt ggcagaagca
     3421 aaagcaaaag atttgattga atggacggac ggaaaagctt tagttggaac aggtatccct
     3481 gctgctgatg tggaatataa cggtgtaact taccaaatcg gtcaagcaaa taatgcgttg
     3541 atgtacccag gtttagggct tggtttgatt gcttcaacag ctacacgagt aaatgctgaa
     3601 atcatttctc aagctagtcg agcattaggc ggaatcgttg atgtaacaaa accaggagca
     3661 gctatcttgc ctccagtagc aaaaatcact gaattctctc aaacgattgc tgaaactgtc
     3721 gcaaaatcag ttgttgcaca aaaattgaat cgtgaagaaa tcacggatat caaagaagct
     3781 gttgaatctg ctaaatgggt tcctgaatat aaatctttag aagattaaga ttgttatagt
     3841 aaaagaacat acagaaactt tgtatgacgt ctaaaaagaa aaaagcgact gcggcaaaac
     3901 agcagccagc caactactgc tcttttgccg cttctctttg gcttttttca tacgatacag
     3961 aataagaaag gatctgtaaa aatggcacaa aaaacaacaa ccgcaactgc agttgaggaa
     4021 aagaaaaaag aagggttttg gtcaacaaaa attaccggcg taagtttgcc cctttatttg
     4081 atcatggtcc ttgtcctgat cattgcaatg gctttaggaa aattaccgac gaatatgatt
     4141 ggcccaatcg ctgttttggt cattttcggt aatttatttc attttatcgg aacaaaaatc
     4201 cctatcgtca aaagttattt aggtggtggc tccgtttttg cgatctttgc ctctgccgcg
     4261 attgctacat ttgggatctt acctgagtat gtagtaaaat ccacagaaaa ctttgtcagc
     4321 aatatgggct ttttagactt ttatattgct gccttgatta ctggtagcat ccttggtatg
     4381 aaccgtaatc tattgatgaa agcttctgtt cgttttattc ccgttgcact tatttcaatg
     4441 gtcgtctcct tttttgccgt aggactcgta ggtatgctta tcggaaatgg ttttgcaaat
     4501 tccgttttat atatctcatt gcccgcaatg gctggtggtg ttggagccgg agctgtacct
     4561 ttatcaacga tttatgcaaa tgttttaggt acggacgctt cttctatcat ttcccgcctg
     4621 attcctgcga cagcaatgac aaatgtccta gctatcattg gtgcagcgtt agttgctcgt
     4681 gcgggacaat caatgccaaa attcaacggt aatggtaaat taatggaaaa aggcgacctt
     4741 ggcgatggaa aacctagaaa tgtaaaacct tccattccac aattaggtgt cggtttgatg
     4801 gtatcattga ctttcttcat cttaggacaa atctgtaact attttgttcc taaagtccat
     4861 gcttacgcat tcatgatcat tatcgttgtt atctgtaaaa tcgcaaacct attaccagaa
     4921 tattacgaag atgcagctat catgttcaac aacttgattg tcaaaaactt gacagcagct
     4981 gttcttgccg gtatcggtat tgcgttattg aatctaaatg tattggcttc tgctttgaca
     5041 tggcaatttg ttgtcttatg tttgacaagt gttatcgcga tttcagtagt ttcaggattt
     5101 gtcggtcgtt tgtttggttt atatccaatc gaatcaacta ttactgctgg cttatgcaac
     5161 aactcgatgg gtggtacagg aaatgtagct gtcttatctg cagctaatcg gatggaactt
     5221 attgcttttg ctcaaatggg caatcggctt ggtggtgcca tcgttttgat catctcaggt
     5281 ttcttgatgc aattactttc ttagtcaaaa aaaatacctg atcatcaagt atcggcttga
     5341 tgaccaggta ttttttgata aaaatttaca ggattttcaa aaatcttttt tgcattttat
     5401 cggaatgata accgatgcgt tcataaaatc ggtgtgcttc tactctgtat tctgcagaat
     5461 taagtcttat tgctgatatt cctcgtcttt tcgcttcttc tacgccagtc atcaaggcct
     5521 ttccgattcc tcttttttca gcatgtttag acacagcaag tgccataata ttgaacatcg
     5581 gatcgctgaa aatcgattca taggactccg cttgtacata gccagctgct tttttagaag
     5641 taggatcttc atagaccaag aaaaaatttt tttctggctc cttcaacagt ttcttcattt
     5701 gtctcaccgt aacggttaat ggtacatcat atcctaattg ttccgcattg atttctcgta
     5761 gtgcttctgc atctcctaaa cgcatttccc gcaacatcca aatcaccagc caatttttat
     5821 ttttttaaat gaattttttt atcacagtat actccttttt tcatttagaa caatatgttt
     5881 cagtgatttt gacttgcgtt agtctagatt ctcctaatgt ttgtaagatg acaaaggcat
     5941 tgtaacaatt gaccaaaaga accacgagga caattacttg aaaaataaag gtcacttttt
     6001 ttggagacaa aaccctgcta aatcttgctc cccacaaacc tccaataaca gcagcgggga
     6061 taacataaaa caacatagat aaatcaaagc gcataaagct acttgttaaa gcaatcgtta
     6121 ccaattttgc cagttgagaa aagaaaatcg tagatagaga atatagtgtt gcttctttta
     6181 ttggcaatgc aaacatcagc atcaacaaag aaacattgac tggtccacca ccaataccta
     6241 aaaaactagc taaaaagccc agaacccccc cacatataag gtaccaagtc catgacgtca
     6301 aatgaaattt tggctgatga tgcttggtgt agaagaacgc aaaaaccaag gtcaataccg
     6361 tcaaaaaaat ttggatcagc tgcacatgct tctcgttttc aaataactgt aaaaaaactt
     6421 cgaatacaat gttgccgact actcctccaa caacagctcc actagatacc caaccgacaa
     6481 tctgccaatt cagtgatttc ccgcttgata cttgtcgcac agtcgaaaca atggacatcg
     6541 tgaataccgc aactgttgag taaaaagaaa tccctgcaac agaatgggcg ccgataagat
     6601 caagaatcgg tttgatcaac acgcctccac ccatcccgga aatagcgcca actgtgttcg
     6661 ccagaacgat caccacaaaa taaatcagac caatcatctt gtatttcctt tctcttgttt
     6721 atatctttta gcgattgtta ctcttttctt ttattataca gtaaaagaaa caaacgaggc
     6781 ttcgaaagct tttctacttt ttaaggaaat ttagtgctaa acgaaaagag gttgtgaaaa
     6841 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagctcctca taattttcgt
     6901 ttggttgaac ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggtcgtg
     6961 agcctgccgt tcagctccga gtcacaagga acaatttcaa aaaacagctt ctcatgtttg
     7021 ttgaatattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa ggaagagact
     7081 gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag ttcttcatat
     7141 ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt atttggaaaa
     7201 agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta attcattctt
     7261 ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa ctataaaggt
     7321 tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt tttcaagcga
     7381 tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt gggggttttt
     7441 aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa tccacttttt
     7501 caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta aatagatttg
     7561 actctggact tataaagtct acttcacctt ctagtattac tttcttatgc aaatattcct
     7621 ctaataaata tagacttgct aaagcataca acacattttc caaatttgct tccttataat
     7681 ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt gtatcttcag
     7741 gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata accctaacac
     7801 cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca gttatgcttt
     7861 tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac tccgaaccaa
     7921 tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc tcatcgagca
     7981 tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac ttccaataaa
     8041 aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac aaaaaaaaca
     8101 cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa ccatccaatc
     8161 agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt cttagtccag
     8221 catgtttgca accacaccta caattttcag tccaacaata gatttcgact agctaagtct
     8281 tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa tacccacaac
     8341 gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta taatctttaa
     8401 acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa gctactttca
     8461 tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac gttccttcaa
     8521 gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca ttagcccaac
     8581 attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt acccgttttg
     8641 tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt ggcataataa
     8701 gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc gctgctggct
     8761 tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc aaattgctga
     8821 taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat atattcgcct
     8881 gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat ccgcatactt
     8941 ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag gcagtgggat
     9001 aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa actcaatata
     9061 cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg caagtccttg
     9121 tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag caacaaagat
     9181 agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat tggcaaacaa
     9241 tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag gtgctgtcac
     9301 atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt ggtaatatcc
     9361 gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat tgtatagttg
     9421 tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata aatccatgat
     9481 atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct cttgtacaat
     9541 attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag cacgagtttc
     9601 tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt cagcaagttc
     9661 agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac cttgtccatt
     9721 tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt tcaaatcaaa
     9781 gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag tgactccatc
     9841 acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg ttttttcgtt
     9901 atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag tggattctaa
     9961 gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag aacgcttatt
    10021 tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt cattaagtgc
    10081 tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga atgaatctaa
    10141 tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat tctcatacac
    10201 atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg tatcatcgac
    10261 attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat cgtgtccata
    10321 taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa tcacttctac
    10381 aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg aaatagtttt
    10441 gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg ctgttagctc
    10501 agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt gtaactcctt
    10561 cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc aaacttgcta
    10621 gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc tcattaatcg
    10681 tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca ggatcagtct
    10741 ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct aattcactca
    10801 caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta ctttgaacgg
    10861 tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac gccttggcaa
    10921 tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg tttatttcta
    10981 atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta tctccactac
    11041 ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat ccgtctaaat
    11101 ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag aaattgtgca
    11161 atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa agtggtggta
    11221 atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta gacttcacac
    11281 gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga gtaaccgtta
    11341 cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt ttaatctctg
    11401 caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa tgccaaccat
    11461 ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca gcttgtggca
    11521 tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata attcgaacgg
    11581 ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg gtgtaatcat
    11641 ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt tgtctcgctt
    11701 taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc gctaccccaa
    11761 taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag gtgatagcaa
    11821 ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta gaaagaaaat
    11881 tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt gaagtaatcc
    11941 attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt actgttcttt
    12001 gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt cactgcatgg
    12061 gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc tctaaaagtt
    12121 cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg gtagttggct
    12181 ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat ccccatacat
    12241 tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc atagtccacg
    12301 attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc tcgataaggc
    12361 aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa gctatgtgaa
    12421 tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt taaatcatct
    12481 gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg tggttctact
    12541 tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg tctccataaa
    12601 ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag gttcacatca
    12661 tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat cggattggct
    12721 ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca tatcgctata
    12781 aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa gtattcctcg
    12841 tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa gccgttcttt
    12901 tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc ttcgtgccac
    12961 tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc actagccagt
    13021 gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat tttcagttgt
    13081 ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag catatcatag
    13141 cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc ttctgctggt
    13201 tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt tttcccattc
    13261 ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc tttagtacgc
    13321 cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat tgctttaccg
    13381 tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc ttcatcgaac
    13441 acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg ctcacacgct
    13501 tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat gtaatcattc
    13561 atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc ttgttcaaag
    13621 ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt ttcaaatcac
    13681 tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg ttttcgcatg
    13741 tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga gcaaaggcta
    13801 aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct ttttttaatg
    13861 ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca gggggcgtga
    13921 tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg attgcttttt
    13981 cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc atgttgtcct
    14041 cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt cgtttttcga
    14101 tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct gcatttgaat
    14161 ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt tatcagtaat
    14221 aacaattgta tttttcaata aatctcctac tttcattata tactttttca cttgtgatta
    14281 caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta tttgtaccaa
    14341 aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact caaagggtac
    14401 tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca tggcataaag
    14461 aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca attttgtgat
    14521 ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat actggatgat
    14581 ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat tgaaaatatt
    14641 taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct ttatgcttcc
    14701 cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta cattgttttt
    14761 taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct tgttcccatt
    14821 ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct ttaaaattaa
    14881 ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga gtgtttgaat
    14941 actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata attattctac
    15001 ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga acttatcatg
    15061 gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg ctcgatcata
    15121 taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat agttgccact
    15181 atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct gcagccaatc
    15241 aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat cttctgttac
    15301 caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat ctgtatattg
    15361 aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc cttgattcgt
    15421 aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa cggggacaag
    15481 ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt ccccagcata
    15541 ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac caaattgctg
    15601 tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga cccgttacta
    15661 cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt gacttattgt
    15721 aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca ggaatactgc
    15781 taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat aaatcctttg
    15841 cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca actaaataaa
    15901 actcttgttg catagtagct ccattataga aatgctgtaa atttttctca ttacatttac
    15961 tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct tcccatttat
    16021 acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc tccatcttgg
    16081 catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg ggactaaaat
    16141 agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga agaaaatatt
    16201 catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa ggataaaaac
    16261 ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca ataaatacac
    16321 attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta tcgatcttgg
    16381 cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat aattctccaa
    16441 gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca cctagcgaat
    16501 ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta ctcttaccaa
    16561 tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt ttatagatac
    16621 gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg ttgtcgtcta
    16681 cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat ttaacttttt
    16741 cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact actgctttgt
    16801 gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc gttaaggcat
    16861 cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa tcttttaata
    16921 ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg ctatcattgg
    16981 ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct ttattgcatc
    17041 ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg ctggcttcgg
    17101 gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc cctcaaatct
    17161 tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg tggttcattg
    17221 tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc gtgccatagt
    17281 aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc gccttgaaca
    17341 atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg agtctttatt
    17401 tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc aatattattt
    17461 agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat aatcatagtt
    17521 ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt acctttatca
    17581 tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac accatcagga
    17641 attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt agaaggaaat
    17701 ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt ttcagaatgt
    17761 tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat atttctagtt
    17821 gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc attccaacca
    17881 ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga ccatcccgac
    17941 ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct cctttttcaa
    18001 aatactggta taaatcattc agacacctcc atgagcttca actgctcaag ttcgtatttc
    18061 aacccatccc gaacaaattc agcacctttt aattgattct gatactttac ccatgtatta
    18121 aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt taaaatctga
    18181 ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt tttgagtaca
    18241 tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc gatattattt
    18301 tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag ttggtttaat
    18361 agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc gtactcgtca
    18421 taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc aattaatggt
    18481 aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg tttcataatg
    18541 gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt tttatcccaa
    18601 ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt ctttgttctc
    18661 gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa attttctcac
    18721 tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact gtctcatcta
    18781 aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt accattttgg
    18841 aatgaccata aatagttgat acagctttat tggctatcac attcgcttta atgtagtcta
    18901 ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt tggtgttctt
    18961 tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt agttctttga
    19021 tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt ttgaatgcca
    19081 tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc caatgaatcg
    19141 atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct agtaacctgt
    19201 ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc attaatatcg
    19261 acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc tccagttcaa
    19321 gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga tactcttcaa
    19381 tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc aatactccgc
    19441 ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac aaacattcaa
    19501 acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg tcttctgtaa
    19561 gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt attatttcct
    19621 cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc cataaagcga
    19681 tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt taaaccgtta
    19741 ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc agcttgtgcc
    19801 ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa cgttccttga
    19861 gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg atcatcaagc
    19921 gtgagcttgt caatttttaa ttccatattt atcccccctc gttagcatac atttctatac
    19981 aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct tataaactca
    20041 atataccatc ataccttttc atttgcaaga attttaacat acttttttga acttttatgt
    20101 ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg tgaaaatgtg
    20161 gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact ggctagaatt
    20221 gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc cagtaaaggt
    20281 gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct taaacaactt
    20341 tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac attaagagat
    20401 atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa aaaaactatt
    20461 gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa catcgaagaa
    20521 gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata tcttaaaaca
    20581 ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc agctcttcta
    20641 aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac agatgagaaa
    20701 aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc tcttgttact
    20761 gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg cccccacggc
    20821 ttgctacact atggagggaa aactattatg gcaacattca agcaatatga aactaaaaaa
    20881 ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg caaacctgat
    20941 aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt gtcacgcttg
    21001 caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt taaagaagtt
    21061 tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt catgaaaact
    21121 gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt gaatgaagtc
    21181 acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga ccaatacaag
    21241 gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg gattattgag
    21301 actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca taaagaaaag
    21361 gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa cagcaaacct
    21421 gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta tagcggggtt
    21481 aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga aaatacggtc
    21541 accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga tcctaaaact
    21601 gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt aaaaaaatgg
    21661 cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac tatgattttt
    21721 tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt gattactatt
    21781 tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac tcatgcttca
    21841 atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg gcatacaaaa
    21901 attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga aaagactgtt
    21961 gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc atacagtaaa
    22021 aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat agtctattct
    22081 tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc tgcgtctgtt
    22141 tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt
    22201 aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata gttacgtcca
    22261 aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt tccttggtca
    22321 tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg ttctgtcata
    22381 agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc taaataattt
    22441 tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc tcttgaagta
    22501 tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc atcttgataa
    22561 tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga agcttctgcc
    22621 cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg gtctacttca
    22681 tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc ttcattgatc
    22741 cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc aacacctact
    22801 gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga tttgagaggt
    22861 agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc aaatttcgag
    22921 catcacgata acgaggaatg taggacgggt ctccagaaag caagtagccg acaatctgat
    22981 tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat gtttcgctca
    23041 cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta aaacccatga
    23101 ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt attttactcg
    23161 aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag aaacactatt
    23221 tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca taaagtatac
    23281 gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca taaaaaattc
    23341 gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat tgatcgtgta
    23401 gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt caatgctttc
    23461 tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag gagtgacacg
    23521 gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt cgtgagcaag
    23581 catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt tgttattttg
    23641 accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc cgtcacttaa
    23701 tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt cagtagggaa
    23761 caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat caatatttga
    23821 atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt ccggctgatt
    23881 cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg ctacttgaga
    23941 agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg aatctttttg
    24001 tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca cgatcgcctt
    24061 ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat cttaacatat
    24121 gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa acctgtttta
    24181 agatggctta aaaatacaaa aaagagacgg attcctcgtc tctaatcagt caccactcta
    24241 tctgtaaggt atttattatt attcaaaaat tttcttcata ctcaagacaa tacactgatt
    24301 tcacaaccgc ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa
    24361 gttaaaaaca taagaagttg tttttgcttg gctgttctta ttacttgttg cagagcagct
    24421 ttttcacaac ctattttaag cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag
    24481 ctgggatttt accactaaac tacacccgca atacaaaaac agtatacgcc tcttttattc
    24541 cttcctcaag tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa
    24601 tcaatcaatt attggagaag atgctatgac gggaaaagaa ttagatctgt tgctggacac
    24661 ttgtttactc gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc gggtagaaga
    24721 tacgatgagt cgaattgctt tagcttctgg aaatttccgt ttggtcagct atgtcactca
    24781 aaccggtctc tttattggtc ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa
    24841 ccgttcgatt aatttagaaa aagtagtaaa agtaaatgat ctttcacgta aatatgttgc
    24901 ttcagagcta acattagaag aactttactg cgaactgcaa gcaatcgacc gagaccgttc
    24961 tttctttcct gtctggttgc aaatcgttag cgcggcagtg atcagtggaa caattatggt
    25021 gttatttggc ggaaagatga ccgatttacc tgtgacttta gtcattggag gattgggata
    25081 cgcagcttat ctatatagtt tgaaattttt tagaatcaaa tttttatcgg aatttttatc
    25141 ttctttattg attggcatag ccgctatcta cagcgtacgt tttggcttag gaagcaatca
    25201 ggatctaatc attatcggat gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc
    25261 tatccgtgat ttgctagctg gacattatgt ctcaggagtt tctcgcggaa cagaagcgat
    25321 gatgactgct acgatgatcg gttttgcgat tgcttttatt ttccagcttt tttattagga
    25381 aaggaacaca agtatgggga cgatatttat tcagttttct tttagttttt tagcgactgc
    25441 ggcatttgcc gttattacaa atgtacctag gcgttcattg atttgttgtg gtctttctgg
    25501 tgcttttggc tggatgatct actggatctg tgttcaaata ggcggatctg ctgcttttgg
    25561 ttcattgctt ggttctttag gagtagcatt catcagcgat cttttctcta aacgtttgaa
    25621 aatgcccgta acgattttca atattcccgg gatggttcct ctagtccctg gtggtttagc
    25681 ttatcaagcg gttcgaaatc tcgttactga atcctatcga gaagcggctc attatgcggt
    25741 ccaagcaatt atgattgctg gagcaatcgc tttaggtctt gtattatcag aagtctttaa
    25801 ccataacatc cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg
    25861 gctttttaaa taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt tcagccttga
    25921 gcgatgacaa aatatctcac aaacattgcg acaagtaacc gcaggagcaa taagaagtaa
    25981 ttatgagtat taaaaaaagt tttgtaaaaa tagcttctca catttttaca taaaattgac
    26041 ttaatactga gaaactgact tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg
    26101 atttttgtca cggcccctct tctttattca aattttttcc aaaacgttcg ttgataataa
    26161 acgatccata gagcggaaag tattagaact aaaccgatca caggaatatt cactaaccaa
    26221 aatggataaa acaaagccaa cattccgtat aagacgataa gaataatact gatgatcaat
    26281 gaacccatca tagttgcgac taaacccaaa cttcctgaac ctctgttaaa taattggtta
    26341 atattggtcc agttcagcag cagcaaccga taatctctag cgaaataacg tagtgacaat
    26401 aagtagcagc cgaataaaga accaaatagc aagctaggga taaaaaacaa aggcagctga
    26461 aaagacagtc cgcttaataa tgcgatccca ccagttaaaa aactctgcaa tatccatccg
    26521 aatctgaact tttcttttaa atattggttc atagagattg gcaaagaacg gataaacaga
    26581 aaattttctt gatccaaaga aataagattg ctgataaaag aagtctgatt aactgttaag
    26641 aaagccaatg cgatccctac caggaaaaca acaccgatta acttattatc taaatcagtt
    26701 aattttattt cgcctcctag ggcaaaagca agaataaaaa tgattggagt taacaaggaa
    26761 gaagaaagaa cttgcataat caaattaggt tcttttacta attgagcatt gtaactaaat
    26821 aaaagctgat gcacattttg gttcttttta tgggttcttt ttattttgaa ctcactgttt
    26881 gaatcagtca gttgctctgt aagctttggt actatcagct gttttacagc aaggaaaaat
    26941 cccagagccg tgatagctag tattccccaa cccaataaac cttgttgaga aagtggctga
    27001 ctggcagcat catagaatgg caataatatc gaaatcggat gccgatctgt taactcattt
    27061 tcaatagtcc ccgtttgatg attcatccac atgattccag caaatgcgat cagcatcgag
    27121 ctgccaagca agatactaga aactgttttt ctatgttttt taaacactgc tgttcgagcc
    27181 aaaccaaaaa caatcaaact agccagacaa aaaagaataa tcaaaaatag agaaaacaaa
    27241 aggattgaga taatgatagt aagcaaaatc cctagatcag ctcgccatcc ggttaaaata
    27301 aacatgacta gaagcggaaa aacaaaagga atgatggtca aagcaactac caaaatcttt
    27361 gctgaaaaaa cctgttcttg tccaaaaggc agaggcaaaa atacttgcag gtcatttcct
    27421 tcaaaaaaga tattataaat gactgatata ctttgagtta aacctaacat accaaaaagt
    27481 gccacgtaat aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa catagcaaat
    27541 ccgtaaatca gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag atagatagtt
    27601 aatcgctttc ccttttttcc tttctctctt gctttattcg tgagctgtgg attaacatat
    27661 aaaagattaa ctcgaattaa ttctaataat tgtcgcttat acatacggtc catctcctgt
    27721 cgcttgcctt ccagccattt tcaaataaat cttttctagc gtttcatcag gtgagccatt
    27781 caataattca ctaactgccc cctgataaat caattcacct ttcttcaaaa tagccagttt
    27841 atcgcataac tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg tttttccttt
    27901 tgcggcatga gccttcatca tttctttcaa atcaaatgct gcttgaggat caagtccttg
    27961 caaaggttca tccaataccc aaatatctgg atctggcagc aacgtgccga tcaaaatcgt
    28021 tttttgacgc attccatgtg aaaaacttgc gattgtttca ttttgatgac tatacatatc
    28081 aaaaagcgct gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta attcataggc
    28141 agctgaaatc aagtcccaat attctccagc cgtcaattgt aaaaaaatat ctggggaatc
    28201 aggcacataa ccgatttttt gtttgatact caatcgattt tccgtcaatt tcattccatc
    28261 tactaaaatc gttcccgatg atggttcaat aatactaact aaacttttaa tcgtagttga
    28321 ttttccggca ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga
    28381 caagtctttt aatgcttgtt ttttcccgta acttttactt acattgatga attcaatcat
    28441 ttctgtactc cttttgtttg ttattacttt ttattgtacc cttcgttatt ggtggagtaa
    28501 atgtcaaaaa gagtgcataa caaaaaagga tagaaacgac taggctgcta cttgggttgt
    28561 tctcttctac aggaaaacac tcttctaaca ggtaagatat gagtctttgt tttctttaaa
    28621 atgattcatt ttctatcttt tcttgcataa aattctttag tttatcaata cttctctttc
    28681 ccatctctag ttgttctcca tttgcaaatg ttactatttc gtttactctg ctaatagatg
    28741 taatgttttc agtattaatg atataagctt ttaaatcttt acataaatac tcatattcga
    28801 gtgatttttt atatttttta attgtgccgt ttacgagaat ctcttcatct ttcaatacta
    28861 ggcctatttt atttttataa cctggcatgc tcattagata aaggatttca tcgtaatgga
    28921 tatagtgatc gctattacca taacggacag ttgtccacct ttttttcgta gcatgttgaa
    28981 caggatagtt ttctttttct ttcaataagt aactttggat gcttttttta tttttttcat
    29041 aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata acagttattg
    29101 ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt tctctaagca
    29161 tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg aaaaaaatat
    29221 ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg ttcaatccct
    29281 gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta
    29341 gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta
    29401 cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag
    29461 gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac
    29521 tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc
    29581 cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt
    29641 taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca
    29701 actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct
    29761 attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca
    29821 aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta
    29881 ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat
    29941 tcgctagggt aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg
    30001 atagtttgct tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat
    30061 ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct
    30121 actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga
    30181 ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt
    30241 ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct
    30301 gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc
    30361 attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa
    30421 acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt
    30481 tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga
    30541 taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat
    30601 tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga
    30661 tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat
    30721 gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt
    30781 ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc
    30841 gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt
    30901 cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt
    30961 gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag
    31021 ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc
    31081 gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa
    31141 cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca
    31201 ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt
    31261 ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat
    31321 aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact
    31381 catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag
    31441 ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt
    31501 tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa
    31561 attaaggtct catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca
    31621 ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt
    31681 cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac
    31741 attatgatat tccggcactt catttggatg catttgaaac accagaagta gctagtaaat
    31801 ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc
    31861 aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag
    31921 atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa
    31981 aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc
    32041 tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa
    32101 ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc
    32161 ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc
    32221 tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga
    32281 catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt
    32341 cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa
    32401 agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa
    32461 tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa
    32521 ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact
    32581 taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata
    32641 atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc
    32701 tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa
    32761 atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc
    32821 tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta
    32881 tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca agccaatcag
    32941 attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca
    33001 ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc
    33061 attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc
    33121 ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg
    33181 aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt
    33241 agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg
    33301 caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg
    33361 catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga
    33421 cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta
    33481 gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa
    33541 agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc
    33601 aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa
    33661 tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa
    33721 acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca
    33781 aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa
    33841 ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc
    33901 ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat
    33961 ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg
    34021 acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg
    34081 acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg
    34141 atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct
    34201 ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc
    34261 ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt
    34321 ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg
    34381 atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc
    34441 gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat
    34501 tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag
    34561 ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa
    34621 aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc
    34681 gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac
    34741 gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa
    34801 ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac
    34861 caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat
    34921 tccatattta cctttttcac caataagaag accaattact agtcattctc actaaagctt
    34981 ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat
    35041 tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca
    35101 attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata
    35161 gacctgattc tattaattct aataatccac aaaccatgat caccatacta gatgtatctt
    35221 tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca
    35281 accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca
    35341 aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg
    35401 aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca
    35461 tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa
    35521 tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca
    35581 ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag
    35641 gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg
    35701 cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg
    35761 ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc
    35821 tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct
    35881 tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc
    35941 ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga
    36001 tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt
    36061 tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa
    36121 acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt
    36181 gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt
    36241 ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg
    36301 ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa
    36361 gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa
    36421 acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat
    36481 gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata
    36541 ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca
    36601 agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca
    36661 tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt
    36721 cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc
    36781 catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc
    36841 aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt
    36901 caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt
    36961 agttccttca cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg
    37021 tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg
    37081 ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa
    37141 tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa
    37201 tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg
    37261 ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata
    37321 gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc
    37381 tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg
    37441 acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa
    37501 tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac
    37561 aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa
    37621 ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg
    37681 atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat
    37741 ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa
    37801 tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga
    37861 gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat
    37921 ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc
    37981 aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct
    38041 ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa
    38101 taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg
    38161 cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg
    38221 agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct
    38281 aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt
    38341 acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact
    38401 gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg
    38461 aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc
    38521 ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt
    38581 gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta
    38641 gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata
    38701 ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa
    38761 atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg
    38821 ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga
    38881 tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc
    38941 tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa
    39001 ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa
    39061 gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct
    39121 acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc
    39181 ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta
    39241 ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac
    39301 ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt
    39361 aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt
    39421 atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat
    39481 aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat
    39541 cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact
    39601 gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc
    39661 tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt
    39721 aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat tccccataaa
    39781 acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact
    39841 gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat
    39901 ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat
    39961 ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa
    40021 ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat
    40081 ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg
    40141 tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat
    40201 tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg
    40261 ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg
    40321 gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt
    40381 cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt
    40441 tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat
    40501 ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag
    40561 tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa
    40621 tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag
    40681 caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat
    40741 tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa
    40801 caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa
    40861 tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt
    40921 tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag
    40981 attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac
    41041 ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa
    41101 tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca
    41161 gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg tcctccttag
    41221 tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg
    41281 cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt
    41341 tgtgccactt gtggattttt tgcatcttta gggacataaa aacttgatcc tcctgatact
    41401 gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca
    41461 gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt
    41521 gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag
    41581 tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata
    41641 tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct
    41701 tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt
    41761 gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca
    41821 aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc
    41881 gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca
    41941 tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc
    42001 acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc
    42061 aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag
    42121 tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc
    42181 agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg
    42241 atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta
    42301 atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt
    42361 tcaagttctt atataaaacg ctttcactta tgtatattaa cggaattagt aacacaaata
    42421 tttatataaa aaaatatttt acttgtcgtt ttggcttttc atttttgtta taatagtcat
    42481 taattataaa tagaaagctt tttatataaa aagagaaagg aaagtggttc ttttgttaac
    42541 aaacaaccaa tcctccaatc ttgtcaaaaa aacactaacc tattccatag atacccatct
    42601 tcatttatct gctgctccct attttcgact actaataaat gaaagcgcta atttatatgc
    42661 tgaatttgca cataaatcta tagaactaga taatccttcc cttttattca ttgataaaca
    42721 gcaagaagtc caaatctttt ctactcaaaa ggaaacacaa acttttatca ttatccagtt
    42781 ttattttgat gagcaattat tagcacatat tacgggacaa tctttttcta tagatcaaga
    42841 aataacatct atcattactt ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca
    42901 tagtaagcca gctatttcct atgatcagga aatcaattct tttttcatac tatgccaaca
    42961 gatgagcatt catttgcttt ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc
    43021 ttccttacca aggcattatc aacaggaact tgctttattg acaatacctt caaatgagaa
    43081 agtcattcct aaaaaaaagc atattcttta ttcagatgaa ttagttgatc caatcatacg
    43141 atatttagaa gaacatcttt cagaaaattt tttgcttgaa caattagccc aaattttttt
    43201 tatcagtcca gccagcttaa aaaaacattt tagaaaagtg aatggaaaaa gtgttattag
    43261 ctattttaaa gaactaaaaa tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac
    43321 ctatacagaa attgcagata tattaggatt tcattcgatc catcattttt cttcagcttt
    43381 taaaaa
//
LOCUS       DAYYAO010000015        43031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000015 DAYYAO010000000
VERSION     DAYYAO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.016"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..3373
                     /locus_tag="ACHARI_000732"
     CDS             95..3373
                     /locus_tag="ACHARI_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8441803.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3914..5821)
                     /locus_tag="ACHARI_000733"
     CDS             complement(3914..5821)
                     /locus_tag="ACHARI_000733"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8441804.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5951..6826
                     /locus_tag="ACHARI_000734"
     CDS             5951..6826
                     /locus_tag="ACHARI_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8441805.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6906..7880
                     /locus_tag="ACHARI_000735"
     CDS             6906..7880
                     /locus_tag="ACHARI_000735"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8441806.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8060..9205
                     /locus_tag="ACHARI_000736"
     CDS             8060..9205
                     /locus_tag="ACHARI_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8441807.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9342..9686
                     /locus_tag="ACHARI_000737"
     CDS             9342..9686
                     /locus_tag="ACHARI_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8441808.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9957..11243
                     /gene="efmA"
                     /locus_tag="ACHARI_000738"
     CDS             9957..11243
                     /gene="efmA"
                     /locus_tag="ACHARI_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8441809.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11381..11980)
                     /locus_tag="ACHARI_000739"
     CDS             complement(11381..11980)
                     /locus_tag="ACHARI_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8441810.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12384..13505
                     /gene="mnmA"
                     /locus_tag="ACHARI_000740"
     CDS             12384..13505
                     /gene="mnmA"
                     /locus_tag="ACHARI_000740"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8441811.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13751..15172
                     /gene="arcD"
                     /locus_tag="ACHARI_000741"
     CDS             13751..15172
                     /gene="arcD"
                     /locus_tag="ACHARI_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8441812.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15186..16304
                     /locus_tag="ACHARI_000742"
     CDS             15186..16304
                     /locus_tag="ACHARI_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8441813.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16364..17656)
                     /locus_tag="ACHARI_000743"
     CDS             complement(16364..17656)
                     /locus_tag="ACHARI_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8441814.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17806..18366
                     /locus_tag="ACHARI_000744"
     CDS             17806..18366
                     /locus_tag="ACHARI_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8441815.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18495..18872
                     /locus_tag="ACHARI_000745"
     CDS             18495..18872
                     /locus_tag="ACHARI_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8441816.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18805..20877
                     /locus_tag="ACHARI_000746"
     CDS             18805..20877
                     /locus_tag="ACHARI_000746"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8441817.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21088..22035)
                     /locus_tag="ACHARI_000747"
     CDS             complement(21088..22035)
                     /locus_tag="ACHARI_000747"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8441818.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22281..22745
                     /locus_tag="ACHARI_000748"
     CDS             22281..22745
                     /locus_tag="ACHARI_000748"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8441819.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22807..23850)
                     /locus_tag="ACHARI_000749"
     CDS             complement(22807..23850)
                     /locus_tag="ACHARI_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8441820.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24227..25192)
                     /locus_tag="ACHARI_000750"
     CDS             complement(24227..25192)
                     /locus_tag="ACHARI_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8441821.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25426..26271
                     /locus_tag="ACHARI_000751"
     CDS             25426..26271
                     /locus_tag="ACHARI_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8441822.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26462..27649
                     /locus_tag="ACHARI_000752"
     CDS             26462..27649
                     /locus_tag="ACHARI_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8441823.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27655..28011
                     /locus_tag="ACHARI_000753"
     CDS             27655..28011
                     /locus_tag="ACHARI_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8441824.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28013..28351
                     /locus_tag="ACHARI_000754"
     CDS             28013..28351
                     /locus_tag="ACHARI_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8441825.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28427..28683
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28746..29780
                     /locus_tag="ACHARI_000755"
     CDS             28746..29780
                     /locus_tag="ACHARI_000755"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8441826.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29773..30459
                     /locus_tag="ACHARI_000756"
     CDS             29773..30459
                     /locus_tag="ACHARI_000756"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8441827.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30483..31331
                     /locus_tag="ACHARI_000757"
     CDS             30483..31331
                     /locus_tag="ACHARI_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8441828.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31528..32301
                     /gene="sufC"
                     /locus_tag="ACHARI_000758"
     CDS             31528..32301
                     /gene="sufC"
                     /locus_tag="ACHARI_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8441829.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32314..33600
                     /gene="sufD"
                     /locus_tag="ACHARI_000759"
     CDS             32314..33600
                     /gene="sufD"
                     /locus_tag="ACHARI_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8441830.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33597..34832
                     /locus_tag="ACHARI_000760"
     CDS             33597..34832
                     /locus_tag="ACHARI_000760"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8441831.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34819..35289
                     /gene="sufU"
                     /locus_tag="ACHARI_000761"
     CDS             34819..35289
                     /gene="sufU"
                     /locus_tag="ACHARI_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8441832.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35294..36685
                     /gene="sufB"
                     /locus_tag="ACHARI_000762"
     CDS             35294..36685
                     /gene="sufB"
                     /locus_tag="ACHARI_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8441833.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36780..37922)
                     /locus_tag="ACHARI_000763"
     CDS             complement(36780..37922)
                     /locus_tag="ACHARI_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8441834.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37961..38695)
                     /locus_tag="ACHARI_000764"
     CDS             complement(37961..38695)
                     /locus_tag="ACHARI_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8441835.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38927..39202
                     /locus_tag="ACHARI_000765"
     CDS             38927..39202
                     /locus_tag="ACHARI_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441836.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            39300..41297
                     /locus_tag="ACHARI_000766"
     CDS             39300..41297
                     /locus_tag="ACHARI_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441837.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(41373..41708)
                     /locus_tag="ACHARI_000767"
     CDS             complement(41373..41708)
                     /locus_tag="ACHARI_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8441838.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            41836..42050
                     /locus_tag="ACHARI_000768"
                     /pseudo
     CDS             41836..42050
                     /locus_tag="ACHARI_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            42087..42449
                     /locus_tag="ACHARI_000769"
     CDS             42087..42449
                     /locus_tag="ACHARI_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8441839.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            42495..42821
                     /locus_tag="ACHARI_000770"
     CDS             42495..42821
                     /locus_tag="ACHARI_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441840.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        14109 a         7683 c         9022 g        12217 t
ORIGIN      
        1 acacttatta taattttgtt ttttattgac agtaaaaata aattaggtaa tactttaagt
       61 aaacgctttc taatttgtta cgggaggaat acacttgtta aaaaaacgta taaaatacag
      121 tgcgctaatg atgaatctcc ttatgctcag taccccggtt ttaatttctg tcgttgaagt
      181 tgctgctaac gaacaacagt ctgtgaacga agaaaatgca cacgtgaaca ttgaagggat
      241 gtcaacaatg gaggaaatac caagaactag tgaagatatg gatacggaag aaggaaatga
      301 tcctatgatg aaagaatcag aaaatgaaaa gacaaaaaag caatctgaag aagcaaatga
      361 agagtggaaa ggggttgttt tcggagaaag cacaagtgct agtaggtatg ccattgaacc
      421 attagaagac ggcgtgcggc tctcttccac gaataacggc ggaaaattcc agtcaagcgg
      481 atcagatgga atgacctatt attatcaagc aatccccaaa gacatcaact ttaggatgcg
      541 ggcgacgatc gaaatagaat cttggaccta tacgaatgcc caagaaggat ttgctttaat
      601 ggtaagagac gcagtaccaa aaaatcattt gtttgggcag gcattttatt caaattcatt
      661 tgccatacta ggcagtcgga tcgaatatgt ctgggactcg gatagacaag aagtggtcaa
      721 cacgagcggc agcaaatata cgatgcgact tggattgggg acacgggcaa tcacaggaat
      781 atcatctgaa gatccgcaag ctgcgccagc tcctgggagt gtctcagtgg aaacaacacc
      841 tttagagact tcagcagggt tcttggaaaa agaaacaggt tcttataata tattcggaaa
      901 cggtcatggg aatctgtttc cggccacgaa ctcgattact aaactggacg tagagatgaa
      961 aaaaacgaat actggtttag aggcatatta ttacgatcct gaaacaggag aagaaatggc
     1021 ttctgacatc atgtatgact gggaaaaatt atacgcttca gatgaatcgg ttatttatgc
     1081 aggatttgct gcagcaagga atatgacgat caaggtagaa ggaatcgaag ttgcttactc
     1141 agatcctgcg acagatcctc ctgggcaaga aagacctact cgcttgattg atccgattta
     1201 cactgtgacc tctagtaatc attcaggaaa ccaagatcat actatgtcct ttagagcaaa
     1261 tgctgacgga ttattgacaa ttaaaaataa agcaacagga gaggtgctta agaacgcaaa
     1321 aaacttggct atcactgcga atcgggaatt tgattatcag acaaaactga tggaaggaac
     1381 gaacgaattt actttcagtt ttacgccaga taaaaactat ccgccagaag aatacgtaga
     1441 gatgactagt tatgaaacaa aagagattct tcatacagtg gactaccgta aacctaaata
     1501 ttcgacagtt tatgtaacac cagaagggtc agatgcagga aacggaagca gacaaaatcc
     1561 attatctctg acacaagcat tgagatacgc atatgctggg cagacaatcg tcatggcgcc
     1621 tggaacttat tccttcgaaa gaggattgac gattccaaaa ggcgttaacg ggacaagaga
     1681 aaacccgatt cagttgattg cagagaaaaa tccagaaaac aagcgtcctg tacttgattt
     1741 taaaggaact ggaaatggga tgacactgtt cagtcactac tggggtttac gagggtttga
     1801 cattacaaat agtgcagcta tgcaaaaagg attacagatt tctggaaatc ataatttgat
     1861 cgaagagatt catgcttatc ggaatggaaa tacaggaatc caaatcagcg gtagctcaaa
     1921 tgatcctttc gaagcatggc ccgcgcataa cttagtcaag aactgtacat cttttgaaaa
     1981 tgccgatcca ggatttgaag atgccgatgg atttgcggcc aaactgacaa taggagaagg
     2041 aaatgtattt gacggttgta tcgcgtatca taatgcagat gacggttggg acctttttgc
     2101 taaaaatgaa acaggttcga tcggaaaagt aatcatcaaa aactcagttg cttatcgaaa
     2161 cggctgggta ccaggaattg agggagaagg taacggtaat ggattcaaaa tgggcggttc
     2221 ttctttaacg ggccctcatg aattgatcaa tagtttgtct ttcgaaaatc ttgcgaaagg
     2281 aatcgattcc aatagcggaa cagatattat cgtcaatagc agtacaagtt ttaataacgg
     2341 ttcatataat gtggctcttt atacctcttc agcaggaaac acagactacc atgcttctgg
     2401 tatcttatct ttccgaacag agaatcttga gatgagagag cagatccgcc cattgaatca
     2461 aaacgaagat caaatctacc actcgttgaa ctactattgg aatgaacgag agaaacaatc
     2521 agaaaatact cttggacata ccatatcaaa agattggatc gagtctctaa atacatgttc
     2581 gaaagaaggt cagcagcctg ttacgcgcta tgaaaatgga agtatccatg ttcatggact
     2641 gatgcagatt aaaccgggga atcgtcaaac agaaaaggaa agagtggatg gtcttccatt
     2701 atctgtaggt gctatttttg acggagaaac ctcaccgtct tcggaatatc cagagtatgc
     2761 aatcgtacaa gaaccaaatc agacaaaacc aaatgaacaa aataatccga ttattataca
     2821 agaagcaaat ggtgtggcgg agaatgcaga aggaaatatc attgaactga agccttttac
     2881 agtttttgtg tcaactttca caccaaatca aggaacgcct gtctttgaag atatgcgtta
     2941 tttcccagaa aagatccaaa tcgttgaaac acagtcaagt gctcaatttt ctaatcatac
     3001 cgctattttt tcagcacctt caaaaggaga atatacgcta gaagtcactt atcgttcgga
     3061 aatatttgat ggggaaaaat ggtctgctca tacagaatct gttacccagc aaaaagcgat
     3121 cactgtgcga gaaactgccg atgtgccccc gggaaatgac aaaccagaaa gagaaaatcc
     3181 aggaaatgaa tcaccaaatg gaaaaccaaa tggaaaacct tctcatccgg ataggacagc
     3241 aaaagaaagt gaaaaaaatg gcactcaagg actcccaaaa acgggagaat ttaataaccc
     3301 gttgctagca ttagctggag gaattttgtt aatcggtgta gttgtttatg tcatgaaaca
     3361 gcgaaaaaaa taagtggaat ttctcctttt atgatgtaaa acaattgact gaggaaagta
     3421 aaaatagaag gaaatgagac caatagatgt ctcacttcct tctatctttt tatagatgag
     3481 gttctccttg ctgcttttga ttggctgttc ttattacttg atgcaggaca gctttttcac
     3541 aatctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca acaaacggca
     3601 aaatatgagg aactattttt ccgttttttt ctctaatact caggtcagga cgctgatttc
     3661 acaacctctc gataaacggt gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc
     3721 aaaaatgtgg aaagccattt tcacaaaatt tttcttacta cttgaaacta agcagctttt
     3781 tcacaacctc taatacatgg tattccagaa gtaacttctt cgaaaaataa gccgaaatgt
     3841 cacaaaaatt tgagaagcga ttttcccaaa ttccttctta tttcttgaag ctgaccactt
     3901 ctggcacaac ctcttaccag attcgcactc ttttttctgg cgctagatac atagcgtctt
     3961 gttccgtgat accaaatgtt tcatagaatt cgtcaaggtt ttttggttgc acgtttgctc
     4021 ggagtttagc aggggcatgg acatcgattt gcagtaaaag ttgttggtat tctttttttg
     4081 cttttgtacg ccaaatcgtt gcccaattga caaagaattc ctcgatagaa agatctgatt
     4141 ccgtttttgc tgcttccaat gcacagctta gaccgcctgc atcagcaata ttttccgaaa
     4201 cggttaattt tccgtttact tttccgtcag caaaaggcag accatcgaat tcttcgatca
     4261 ttgcttgtgc tttgttttga aattgtgtaa gatcttcttc tgtccaccag ttgttcaagt
     4321 tgccatattc gtcaaataaa gcgccgttat tatcaaatgc atgggaaatc tcatgagcga
     4381 taacagctcc gattccacca taattagcag agcttgattg ttctagactg taaaatggtg
     4441 cttgaaggat cgctgccgga aagacgatca cattccgaaa tggatgataa taagcattga
     4501 ccgtgtcagc actcatttcc cattctgaac gatccactgg tttgttccat ttgctgaaac
     4561 gatcttttaa tgtgagtttg ctgaaaacaa gagtatttga aagcaaagta ccaccctgat
     4621 ctgcaggtac tgttttgaac tggtcgaata atgctgggat cttatcgggg tagcctactt
     4681 ggatacctaa gttatttaat tttatgacgg ctttttcttt tgttgcatcc ccaagccagt
     4741 cattttcttc tagacgtttt ttataaacgg cgatcatttt tttcaccatt tgttctacgt
     4801 cttttttagc agcttcgcca aaatattttt ttccgtagta atcgccaact acttgatcga
     4861 attgtccaga ggcaaggtaa tacgctgctt ttttctgagg cattgcttcg tctgttccag
     4921 acaatacgcg agaatagatt ccgctgactt ggcggaattc ttctgataga taaccactaa
     4981 gagaacgtac tgttttaatg atcatccagc ttttgaataa cggaaaatga gcttctgtca
     5041 ggatgtcatt caaatgatcg aagtacactg gatcagttac gatcacttta tctggagttg
     5101 tcccaatcaa acccgtgaca agttttttca agtccagttt atctgtgtat ttaataaatg
     5161 tatcaaaaga ttgcggatta tacattttgc tgtaatctgc gttttcttct gcacttttta
     5221 catgaggagc aatcaattta tcaaattgca ctgcttgttc cgtgattgct tctgcctgac
     5281 ttttttcttt gccggcaagc tctaataact gaacagccat atcaaagaaa acgcctatta
     5341 gttgtgcacc gttgggatgg tctgatgcat aatacgtctt atctggaagg aataaagaag
     5401 gcggataagc aaagaaggcg ttcgtttgcg cattcttcat atctgcatct acatccagat
     5461 taaatggcaa tggtaatgta tccaacagcc attctggaaa ttgcttattc aattcgtcga
     5521 aagactgcaa tgattcaact ttttcgatta taggttgaag cggttgagca tgcaactcat
     5581 ttcttcggtc ataatcgttt gccagttgat aatatgcaag gaaatgtttc attttttctg
     5641 aagacacttt ttctggatgg gcaagcagat cgtctgtttc tcccatcaaa agttggtcga
     5701 tcccgtcaac taaatcttgg aaaccgcctg tagctggttt atctgcaggg atttttgctg
     5761 ttttttgcca ttcttcattg actgcttcat agaaatcttg tttcaatcgt tctttgttca
     5821 ttcatcgatc gctccttttt tgtttcgtgt gcccattatc ttacaaaatg aaggaattaa
     5881 caaatagaac ataaaaaatt gaaaattcga acaatcaaac tgatttgttt ttttcaatcc
     5941 ttcacttata atgaaaaaaa ggaggcgatc ccaactgaat caagtagtgg ataaaccttt
     6001 atatttcaaa gtagataaaa aaattaaaaa attagccagc actcagcagc tgcagtcaag
     6061 aaaaagtaaa ttgctttttt tagctctggt ttttgaagat caaagttatg tgattatcga
     6121 tcaatcagga catccggtag aatattctcc agcaaaatat acttatcaag agggtctttc
     6181 ttgtaaaaaa tggaagctga tcaatgaaga gccaatcgag ttatctagat ggatcaacag
     6241 aaaagaagat attcccgttt tgatcgaaga aaaaaggtca ggaaaggaac tcgcaaattg
     6301 ctgggtggga ttgccagaag aacgtttttt gaggtataag aaatgggcta ccccctcagg
     6361 ctatctttgt ggtacttacg cggcagcagt tctccttgcc tattatcaag actatcgtaa
     6421 ggggtggatg cttccaaatg agattcgcaa gaaaaatacg gctgattcgt tggttttgac
     6481 gaaggcattg cgtagccaga tccaaccact aggacttcct acgattcctt ttcaggttag
     6541 tacagggatt tctagttttt taaagaagaa tggcaatcat gagcgtgcac gagcaactct
     6601 tctaggttca tggcagcgag ctacaaaacg tatacgtgaa ggcaaacctg tgatgattgg
     6661 tattttgaaa gtacttggca gtacatatgg caatcattgg gtgacagcgt acgcttattt
     6721 tgaaactgaa acaggagaac gttattacaa agtacacgac aactggggcg attatcataa
     6781 agtcattcct gccagttgga gtaacggaac agtttcactt ccttaagaat ttgctgaaat
     6841 taacagaaaa tcttggaaaa atatgttaaa ctatcgaaga atagagaagt tttggggagg
     6901 actacatgag taataagtat caggatgaca cgttgaagat ttttagtttg aatggcaacc
     6961 gtccattagc tgaaaaaatc gcgaaagtgt tcggaacaga attaggtaaa agcgtcgtga
     7021 agcaatttag tgatggcgag atttccatca atatcgaaga aagtattcga ggggatcatg
     7081 tgtatatcgt tcaatcaacg aatgcacctg tcaatgatta ctatatggaa ctactgatca
     7141 tgatcgatgc aatgaagcga gcaagtgcga aaacgatcaa tgtcgtattg ccttactatg
     7201 ggtatgctag gcaagaccgc acggcgaaac ctcatgaacc gatcactgcg aaattgatcg
     7261 ctaacttgat cgaagaagca ggggcaactc gagtactgac tttagatctg cacacggttc
     7321 aagtacaagg attctttgat attcctgtag ataatttatt tacgatgccg ttgtttgctc
     7381 attattaccg tcaattaggt ctgacgggag atgacatcgt agttgtttcg ccaaaaaaca
     7441 gcggtgttca gcgtgcacgt agtctttcag aatatttgaa cagcacatta gccatcgtag
     7501 atcatgcaga tgatgcttca actgagggtg gttatgtgat cggtgatgtt caaggaaaga
     7561 catgtatcat ggtagatgat atcttgaaca caggagcaac gcttgcacga gcagcaaatg
     7621 tattaaaaga aaacggcgca aaagaagtat atgcctgtgc gtcacatgga ttattgtccg
     7681 atcatgcgaa aagtatttta gacaatgcac caatcaaaga tatttgtatc actgattctg
     7741 ttttcacaga agaaaatcgt catccagaaa acttaacaat cgttacatgt tcagaattaa
     7801 tgggtgaagc ggtcaaacgc atccatgaaa atacaccgat gagtccgtta ttccgtctgg
     7861 aagaaaaaga attcgaataa acaaaaaaaa gagggaaaga agttgtgaca gaagtgagca
     7921 gcttcaagga gctgcgacaa gacttttgtc acagctcctt ttttctatgc ccatgaaacg
     7981 tcctctagac aaaaatgagc tcttctttgg tttgttcgct attttatgct agaatgtgcc
     8041 tagcaataat ttggaaggga tgaatgaagt gaaaactatt tatctggatc atgcggcaac
     8101 tacccccatg catccacaag taattgaagc gatgacggca atcatgcaag acacgtttgg
     8161 aaatccttca agcatacatg gatttggtcg ccacgcgcat gaaaaattgg aaacagcaag
     8221 acaaatcatt gcagacagtc tgcaagcaaa acctcacgag atcatcttta acagtggggg
     8281 aacagaaggg gataatacag ctatcttaga aacagcgttc tcgcgtcaaa aagaaggcag
     8341 acatctaatc actacagcta tcgaacatcc tgctgttctt aataccatga aatatttaga
     8401 gacaaagggg tttgacgtta catatctgcc tgtagatgaa aaagggaatc tgtcgatcga
     8461 agaggtcaaa aaagcgttac gagaagatac gatcttggtc tctgtgatgt acgggaacaa
     8521 tgaaatcgga aatttgatgc caatcaaaga aatcggtgaa cttttagcgg atcatccagc
     8581 ttatttccat acagatgcgg tacaagcgta cggaaaccaa acgatccatc cagaagaatt
     8641 aggaatcgat ttattaagta tctctgctca taaaatcaat gggccaaaag gcgtcggttt
     8701 cttatataaa cgagacggag taaatatccc tgcattgctt ttaggcgggg agcaggaaga
     8761 aaagcggcgt gcaggtacag aaaatttagc agggatctgg ggtatggcaa aagcagttga
     8821 actgctgtca cctgaagaac gtgaagcacg taacaacaaa tataaaggat tccagcaatt
     8881 gattcttgat acattagaag caaacgaaat cccttatcaa gtaaatggtg atttaacgaa
     8941 taaactccct catgtgttga atcttcgctt aattggcgaa gcaaatgatt tgctactgat
     9001 gaagcttgat cttaatgggg tcgctatttc tactggttca gcttgtacag caggaaatat
     9061 cgagccttca cacgtattag aagcaatgta tggagaagat acaccgatac taaaagaatc
     9121 catccgtatc agctttggtt tgggaaatac cgaagacgac atccagtatt tccttgatca
     9181 attagttcgt gctgtcaaaa aataactagt gaagtcattc ttatatgaac agatgtctga
     9241 gagaacgcct tcatttttta gggaacttaa ctgaaaaacc tagtaaaaat caggataaac
     9301 ttttataata aagagaaact aattttatcg aggtgaaaat catggcattt gaaacaacag
     9361 caacggtact gggcgcacca attagctatc gtttgcatcc tgatgcaaaa agatatacat
     9421 tgcgcgataa tggatttaca gaaacaaacg gaggcaatta tcaattgatc cgaccattag
     9481 atgccacgcc gcaaagcaaa gaaggattca aattaaaaat cactgtatca aaagatattc
     9541 aaacattgaa aatgtcgatc actacagcaa atggattgaa agcagtcaat attttcaaag
     9601 atccaaaaca gaaaatgagc caagataaat tttattttct gatggatggg atgattagcc
     9661 gtgggttatt cgaaaaagtc gaatgatctg tggatataaa aagacaacta gttttgaaac
     9721 ggcctattgt tagatgacta actttgtaag gacgacgcaa attagttgtc ttttttattt
     9781 ttaaatttaa cacatgttgt tatttttaat attttgctga tttattgttt ctaagtaaag
     9841 aaatttaaat tgaaacgatt gttatttagg gaaaaaggta ttttttgaaa aaagaatccc
     9901 gtataatgat tgttgtgcaa acgctataca aaatgaaaaa gaaaagaggg atgtttatgg
     9961 aaaatgaaca gtcggtcgtt ttaacgaatt ggaagagaaa ttatttgttc tttttatcgg
    10021 gacagttttt gtcaggaatc actagtatgg tcgtccaata tgcgatcatc tggtatttga
    10081 caagagaaac cggttcggcg acaattttga gttttgctac tttactagga atgattccca
    10141 tggttttact aagcccattt gtaggaccgc tagtggatcg ctgggataaa aaagctcttt
    10201 tgattgtcac ggatattatt gtagcgattt tcgcgcttat tttagcagta gtcggaacga
    10261 tttcagaatc cttcccgatt tggctagttt ttgtgtcttt gtttatgcgt tcggtcgcac
    10321 aaacattcca aatgccaacg atccagtcga ttatgccgac aatcgttcca tcttctcaca
    10381 taacaaggac gaatggacag ttaggaatgg tccaatcagc gaatttcatc attgcgccag
    10441 ctctaggtgc ggctttgttt tcagtcgttc ctgtcaatta ccttatttta ttagatgtat
    10501 tgggggcagt ttttggtgtc ggcttattga tttttgtgaa gatcccaaaa gtttctcctg
    10561 aaatattgga agttccgctt actattttta aagatgcaaa atttgggctg cagcaattga
    10621 tggataataa agggttatgg tacataacga tcaatggtgc atttgtgatg ctgttgttca
    10681 tgccagcaat cagtctgtat ccattgatga cgctagatta ctttggtggt tccgttggac
    10741 aggcgggagc agtggaagta gtctacgcag tcggaatgct cttaggcggt gcactgatca
    10801 gttttatagg aacatggaaa gaccgaatga aacctattat tatagcctac atcattatgg
    10861 gcctgacgat cggtgcaagc ggattggttc caaacgatag tcaagggttc ttgtactttc
    10921 ttatcttgaa tgcaggagca ggttgtgcaa cgccatattt caatacactg ctgatggcaa
    10981 tgatccagca gagttatgaa tcgaatgttt taggtcgtgt cttagggaac ttcaactctt
    11041 tgatgaacct ggcaggtcca attggattat tatttgcagg accattagct gatcgtttag
    11101 gggtagaaaa aatgttcttg ttttccggaa tcgggatttt attatgcgga atcgttttat
    11161 ttttaacgtc agctgcccga aattatgata aagaattaca gaaaaaactg gtaaaagaac
    11221 accatgagca aaaagacgaa tagtatggaa tggagttgtg acagaagtct tgttacaact
    11281 ccatttctga tcctaatgtt cctgtggcaa taagcctact caaatgaaaa atatgagtaa
    11341 ttatctttat ttgtttaata cttgatatag ggcagttttt tcacgaccta tcgtacttcc
    11401 tctttgcttc atagatttct ttgatttgtt gctcagataa cggagtgcca aatggtagtt
    11461 tttcctttaa gagttctctt tccggtgcgt ctacgcctac attgacatcg ttctcgatag
    11521 gtacgctgta atgatggata tgtccatgaa gattgcggat agtgtcgtta ggcccgagca
    11581 acatcggata atgagtcaag taatattgtt gatggtcgaa tttaacgatg gcacctacat
    11641 cataaaaata aaatttggga cttccgtcag taagcctagg atcatttttt tctaaataac
    11701 ggaaaaaagc gcgactgtca tggttccctt tgatcaagtg gattgttcct tgcagttgag
    11761 tgaccagttc caaaatttct gcatttgctt tttcatattg cggatgcata gcaaggtcgc
    11821 ctagatgata aacatggtcg gtttctttta ttactgcatt ccagctagtg atcaactgtt
    11881 ggttcatttc atccacattt gcaaagagcc gcggagcaaa gtcgtttttt cctaataagc
    11941 gttcatggaa aaagtgcata tcactgataa agtaattcat gtatcttttg cctccctttt
    12001 ttcttagagt aaacaaaatc acttatctga gcaactcata acatctaaaa agaaaaggtt
    12061 gtaaatgaga cgtgcagctt taagcgataa gaagcaattt tgaaaaatag tttttcacaa
    12121 tttataaaat tattccttat tgccgaaagg ctggcccttt ttatgagtat gccaattttt
    12181 tatatcgaat gctcaaaaga tgagtgattt tttggttgac tatcatgttt gtgtactgaa
    12241 taggtccagt agaacagtat ttttacgact tgataattaa gggatgaaat tttcagaaat
    12301 atgacgtata atgtaaaata ctgaaaagtt gcgaccttca actcgtagat gtttcagaat
    12361 ctaattgaaa tgatggtgat atcatgacag acaacagcaa aacacgcgtt gtcgtaggga
    12421 tgagcggcgg tgtcgactca tctgtaacag cacttttgtt aaaggaacaa ggatacgacg
    12481 tgatcggaat attcatgaaa aattgggatg acacggacga aaacggtgta tgcacggcca
    12541 cggaagatta taaggatgta gcgaaagtag ctgctcaaat cgggattcct tattactctg
    12601 tgaattttga aaaagaatat tgggatcgtg tatttgaata tttcctagcc gaataccgcg
    12661 caggacgtac acctaaccca gacgtgatgt gcaacaaaga aatcaaattc aaagcattct
    12721 tggattatgc aatggatctt ggcgccgatt atgtagctac tggacattat gcgcaagtca
    12781 ctcgcgatga aaacggcgtg gtccacatgc ttcgtggagt agacaataat aaagatcaaa
    12841 cttacttcct tagccaatta tcacaagaac agctttcaaa aacgatgttc cctctaggcg
    12901 gcatggaaaa atcagaagta cgggcaattg ctgaacgcgc aggccttgcg actgctaaga
    12961 aaaaagattc aacgggaatc tgctttattg gtgaaaagaa cttcaaacaa tttttgagca
    13021 actatctgcc agcaaaaaaa ggaaatatgg tcactcttga tggtgaagtc aaaggacaac
    13081 atgctggctt gatgtattat acgattggtc aacgtcaagg actagggatc ggtggcggag
    13141 gagattcgca agaaccctgg tttgttgtag gaaaagatct agcgacaaac acattatatg
    13201 tggggcaagg gttccatcat cctgcattgt atgccacaag cttggatgca agtgaaatcc
    13261 actttacgac gaatgaacca atgccaaaag aattcaagtg tacagcgaaa ttccgttatc
    13321 gtcagcaaga cgttcctgtg acagttcgct tattggatga taatcgtgca gaagtcgtat
    13381 tcgacgaacc tgtacgtgca atcactcctg gacaagcagt tgtattttat gatggaatgg
    13441 aatgtctggg tggtggtttg atcgaccacg catatcaaga aacaaaagtt ttacaatacg
    13501 tttaagcgat attaaatgaa tcaaaacagc tatggctaac aaaagaaaca ccttttcttt
    13561 tggatagcat agctgttttt ttgcaataaa attaattttt attcatttta tacttgaaat
    13621 acttgtgtaa aaagttcata atatccaata atatgaataa agttgctttt tgttaagcaa
    13681 gagcagttgc aaaatagaag ggaatctttt gcaagtgatt tgttcaattt tataagtgag
    13741 ggtgatggaa atggaaaaga aatcgacggg tattagcaaa tggggactgg ttgctctggt
    13801 tgtcagttcg tcaatcggaa gcggtgtgtt tgggatcaca agtgatctgg ctagttctgc
    13861 agctccaggg ccagcgatcc tttcatggat aatcgttggg attggtattt tagctttggt
    13921 attatcgctg aatcatttag gagaaaagcg tccagatcta gatggtggta ttttcggtta
    13981 cgcggaagct tcatttggta aattaggtgg cttcatcagt ggttggggtt actggttgtc
    14041 tgcgtggctg ggaaatgttg cttttgcaac gatgctgatg agcgccatgg gagaattttt
    14101 cccgatattc aaaggtggac aaaatgttcc ttctatttta tttgcaagta tttttatttg
    14161 gggtctgact ttattagtaa acaatggagt tgaaggagcg agcgtcatca atacaatcgt
    14221 gacgatctgt aaattagtgc cattattcct atttttaata tttgtattta tcgcatttaa
    14281 agtgaatata ttcactgctg atttttgggg taacgtatct gataatctag tgaatggaac
    14341 agggcagcaa ggaacgatct ggttgcagat taaaggttgt ttgatggtta tgatgtgggt
    14401 ctttgtaggg atcgaaggcg caagtgtttt agccaaccgc gcgaagaaac gttcagaagc
    14461 ccagcaagca tcgatcattg gtctgctttg tttattgttt atctatatcc ttgcttcgat
    14521 cttgccatac ggcgtcttat ctcaagaaga gttagcaact atttcacaac ctgctatggc
    14581 aaatatttta aaaggaatcg tgggaaattg gggagcagcg ctgatcaata tcggcttgat
    14641 tatttcgatc attggttctt ggttgtcttg gacgatgctc ccagcagaaa caacgatgtt
    14701 gatggctcga gacggtctgt tgccgaaagg ttggggaaaa acgaataaaa aaaatgcccc
    14761 aacttattcg ttagttgtta ctgcaatctt gactaatcta ttcttattga ctttcttagt
    14821 tactgattat gcctatcaat ttgcctattc tttatgtaca gcggcaattt tgatttgtta
    14881 tctacttgtc gggatctatc aagtccagtt ttctttaaaa gaaggaatca aaaaacaagt
    14941 agtgatcggt gggattgctg tagtttttga attgatgggc atcattttag caggattttc
    15001 ttatgtcctt ttatgtagta ttgcctatct tcctggtttt tgcttctact ggatcgcttg
    15061 tcgtgaaacc aaacatcaaa ttacgggaaa agaaaaagta atgattgggc tgatttgtgt
    15121 ggccgcactg ctttcaatcg ggttgcttgc tgtaggctgg atcaagatat aagggggaaa
    15181 gaaacatgaa aatcgcaaat ttcggtgtag aagaatggct aaataaatgg gaaaagaaag
    15241 cggtatatga tattgcacaa agctcgatcg aagcattgac attagacgaa ctcgttggct
    15301 tagatgggac tagcgtaagt aaatttttcg aaaaacgcaa aaacgaacca ttggactatg
    15361 gctggatcga aggatcagat gttttcaaac aagaagtcgc ttctctttat caaaccgtcg
    15421 atccggaaaa catcttacaa acgaatggag cgactggtgc taatctttta gctctatacg
    15481 cgttagtgga agcaggagac catgttgttt cgatgcttcc tacttatcag caattatatg
    15541 acatccccaa atctttaggt gccgctgttg actttgttca tttgaaggaa gaagaagatt
    15601 ggcagtttga tttagaacag ctagaagcct tagtcaaacc tgaaacaaaa atcatctgtt
    15661 tgaatagtgc aaacaatcct acaggcacat tgctagatcg gaaaacttta gaaaaaatcg
    15721 cagaaattgc tcgtacagta gatgcatatg tattgataga tgaagtatat gcgcctttaa
    15781 cggataaagg tgaattcttg tcgatcgtag atgtctatga aaaaggtata gcaaccaatt
    15841 ccctatccaa aacctactcc attccaggta ttcgtattgg ctggacagct actggacctg
    15901 agttggcaga agtttttcga aagtatcgag actacacgat gatttgcggc ggagtcctgt
    15961 cagatgatct tgctgtacat gcattgaaga ataaagaaaa aatattggcg cgaaacaaaa
    16021 agatcattac agagaacctt gtgattttaa aacaatgggt agccaatgaa ccgaaagttg
    16081 aacttgtcgc tcccaattac gtctctactt ctttcattaa attagccatc gaagaagatg
    16141 accagacatt ttgtatcaat ttattagaag aaacaggagt actattagta ccaggaagtg
    16201 cctttgattt accgaagcat gcccgactag gttactgttg taaaaaagaa accttggaaa
    16261 agggattatt cctcttatct gagttcttga aaaaacaggc ctaaacaaaa acactgcaag
    16321 gacagtctgt gacaactgtt caagcagtgt ttttcattta cgcttattgt ctatgagaag
    16381 gagagtgccc tgaagctatg gagactcctg aagctttttc ttctgcagtt tcttgctgat
    16441 ggatctcttg tgtattgtgt cgtacaagca agaagttgac caaaacaaga agagaagtcg
    16501 aaagaaagac gccgcgataa ccaaatgcac tggaaacact ggagccgatc ataggcccta
    16561 ctacgttacc agttgcttgg aaagattggt tataactgaa gatccgtcca gcagcttgat
    16621 gaggcgaata ttttgtgatc aatgcttgga cagcagggag cagacaagca tcagagatac
    16681 caatcaaaaa acgtaaggca gctaattgcc aaacattcgt gacgaatgcc attgggatat
    16741 aaaccagaat cgcaaaaccc aaaccaatcg caagtattct ttcactccca atcttgtcac
    16801 ctaagcgacc aaatcttgga gcggctatca aagtggcaat cccaggtatc gaagcaatga
    16861 ttccgctgat caacgtaaca ttcccatgtc catgcagcaa ttggcgaatg tacagactga
    16921 tgattggact aatggaattg ttggatgctt ggatgatcat cgttgtgacg aacataccga
    16981 tgacgacgtg tggatatttc aagtctttga aaatctgttt ggcagaaacc atgttttctt
    17041 ttttcactgg gacaaatttt tcatggacga aaaacagact caacaagaag acgaaaaata
    17101 agatgatccc tgtaatgaaa aaggtagggc gatagccaaa cgctgaagca gagatcccgc
    17161 caagaagcgg tccaagtaat gttcctgtaa cagaaccagt tgctaaggtt ccaagaactt
    17221 gaccactttt ttcttttggc gtcccagttg cgacaagggc agtggcatta ctgatataac
    17281 cagaaaaaat cccttgaagc agacgcaatg cgactaattg atacacacta gtcacgcaac
    17341 ccatcaaaga aatgacgatt gccattccta atgaagcacg caacagcatc aattttcttc
    17401 ctttttggtc ggctaatttt ccccaccaag gagagataag cgtagtcacc aagaaagtag
    17461 atgaaaaagt caatccgctc caaaagttca attgtgacgt actataatta cctaacgtat
    17521 ctatgtaaag tgacataaaa ggcatgacta aactaaatcc gataccagcc ataaatgttc
    17581 caaaccataa gacaaacaag ttttgttccc aagtttctct tttcctaaat aatttttcct
    17641 ttattcttgc agccaattag actccttctt tctgaaaatt ttcatctaca gctctttatt
    17701 ataaactaca atatgtagtt tatcaatgaa agtctctttt ttctctttct ttttcgtggt
    17761 aaactgaaag agtgaaggat ttattttatg agaggaaaca tggaaatgaa aaaaagaaat
    17821 ctgtctcaag agaaaatcat cgactgcttt cgagaactag cagaagagat ggatgttcag
    17881 caaataacct ttcagcattt agccaaacgc ttggatatca aggctccctc gctgtataac
    17941 catttcaaaa atattcgtga tgtcaaaaca gcgctaacag cgaaacttct gcaggaactt
    18001 aatgaccagc tgcgacgggc tcttgtggga aaaagtggtc aggaagcact gcagatctat
    18061 gcagaaactt atcgatcctt tgctttttca aatcaagcag tgtatgagtt actgatcagt
    18121 gtaccgcata ccaatgaagc tgttttatta gatggaatcc atgaaacgaa tcagatcatt
    18181 ttgcagatat ttgaggcttt tcaattaacg aaagaagaaa aagttcatcg aagcagggaa
    18241 cttcgtagta tgatccacgg ttatctttcc ttacgttttt taggatactt tacaaaagaa
    18301 ccaacaatcg atccagaaga cagctatttt tggatgatca ataactttat cgctactttg
    18361 ccttaaacaa tcagattcat ttgtaacaat tttaagatgt tcattaaatg acccttttat
    18421 aagagaaaat tcttgcaact ttcttgaaaa gtgactaaga tgagtctaga aaagctgaaa
    18481 gaaggagcac gagtatgtat caagtaatta ccatgttcgg ggacaatgag ccttggtggt
    18541 tttttgaaga ctgggaagaa gatattgaag aagaagaaac attcgagtct ttcgaagaag
    18601 cacgacaagc gtacgaaaaa caatggaacg aaatccatca aaattatgaa tatatcaatg
    18661 caaaaagtaa ctttctcagc gccttttgga atgacggaga tgagcgctgg tgtgaagagt
    18721 gtgacgatga tttacagcaa tacaagggac ttgcgttgtt gaaaaatcat cagcccatca
    18781 cagtagaaag cagaaaggaa ttttatgaaa caactaattc tagcggaaaa accaagcgtt
    18841 gcgaaagacc taagcaaggt gcttggtgct aatcaaaagc acaaaaatta ttatgaagga
    18901 cccaaggtga tcgttacttg ggcccttggt catttattag gattgaaaat gccggaagat
    18961 ctgaataagg aatggcagag ctggcaaatg gacacgttgc caatgatccc taaaaaacta
    19021 ggaatcaaac cacttcctaa aacaggacat caattaaaag caatcaagca gctggctcag
    19081 cgaaaagatg tctcagaagc agtcattgcg acagatgccg gaagagaagg ggaacttgtt
    19141 gctcgctgga tcttagagta tgtgcatttc aataaaccag tgaagcggtt atggatctct
    19201 tctcaaacag ataaagcaat caaaactgga ttcaagcaac taagacctgc gaaagaatat
    19261 gatgcattgt atgaatctgc tcttgcccgg gcaaaagcgg actggctggt aggattgaat
    19321 gtgaccagag ccttaactgt gaagtacgcg gataatttat cagcaggccg tgtgcagaca
    19381 ccgacactag cattagtgag agaccaagaa aagaaaatcg agcagttccg tccacaaacg
    19441 tatttcacga tcacgttgca agctggaaaa gaacaagcca aaatgcttca aaaaaatcaa
    19501 tttgctttaa aaacacaaga agaagcacaa aaacttgttc aattgatcag tacaagcaaa
    19561 ggaactgtca cagaagtcca agaaaaatta aaaacggaat ctgcgccatt gccttatgat
    19621 ttaacagaga tccaaagaga agccaatcaa cgctatggct attctgcaaa gaaaacatta
    19681 agtcttgtac aaagtttgta tgagacccat aagatcgtta cttatccgcg tacagacagc
    19741 aaatatctga caaatgacat gaaaagtacg atgaaagaac gattgcaagc agtcagcgac
    19801 tttgctcctg aggtcaagca gtatttgaaa aacggagcca tcgtccaaca gacaaaagta
    19861 ttccaagaca gtaaagtgac cgatcaccat gcacttttgc caactgaaaa cagagcacgt
    19921 tatgaaaaat taagtaatga agagcgaaaa atctatcaaa tgattgtctc tcgtttttta
    19981 ggactgtttg ctcaacccca taaagtcagc caaacaaaag ttacggttga atttggtaaa
    20041 gaacgattca tttttcggca aaatcgtgtc gtccaagcag gatggaaagg cgaaactgaa
    20101 actgagacgg aaacagtcaa atgggaaaaa ggaatgagaa tcaatcccga tttcgccatc
    20161 aaaaaagaac tatctgcacc gcctaaacca ttgtcagaag caagcttgct tggtcaaatg
    20221 gaaaaataca gtctgggaac cccagctacg cgtgcagaga ttatcgagaa actaatcaaa
    20281 tctgaactga tggaacgtac ccctcaagga ctgcaagtat ctcctaaagg taagcaatta
    20341 ttagtattgg taaatccctc tttagtaaca ccagatttga cagaaagctg ggaaaaagac
    20401 cttgaagcta ttgctgcagg aaagaaacaa gcaggaacct tcttgaatgg aatcgaaaaa
    20461 gaaacaaaac gcttggtgaa tgagatcaaa tcaagcaaac aagaatatca agatttctcg
    20521 attacccaaa agaaatgccc agaatgtggg gccaatctgc gcgagaaaaa cacacgtgac
    20581 ggcaaaatct atgtctgcac caattcagat tgcaactacc gcagaagaaa agatccaaaa
    20641 gtctctaatc atcgatgccc gcaatgtcac cggaaaatgg aaatcatcga aggtaaaaac
    20701 ggtgcttatt tccgttgtaa atacgatgga acgacagaaa aattgcttga taaaaaagag
    20761 cagaagaaga aaatgaccaa acatgaagaa cgacgcttgc tgaaaaaata tgcacaagca
    20821 gaagaaccag aagaaagccc attagcagca gcactaaaag cagcaatgaa gaagtaataa
    20881 gatatgaaag aggttgtgat agaagtggtc agcttcaaga aataagaagg aatttacgaa
    20941 aattgcttct caaatttttg tgaaatttcg gcttatttcc gaagaagcta cttctggaac
    21001 accatttatt cggaaaaaag gactgtgaca agacttttgt cacagtcctt tttcatttaa
    21061 aaaaatgttt acttcatttg acgtatatta ttggtgaggc ttcaattcat ttggcttcac
    21121 tttttctgtt tctcttatga tataaacagg acgctgtttc gtttctaaga agattttccc
    21181 aatatatttc ccgatgattc caagacataa caattggatt ccaccaatga acaggaagat
    21241 cgagaccatg gaaggccatc ctgatgtagg atcaccaaac aacaatgccc gcaaaataat
    21301 gaacaaaatt gcaataccag aaccgacaca agacaaagcg ccgataaatg aagcaatcgt
    21361 taacggtgca tccgagaagt tcacgatacc atcaatcgag tagttgaata atttccagaa
    21421 ggaccaagat gtttcgccag ccacacgttc gcggttttca taagaaatat attctgtttt
    21481 aaatccgacc cagctaaata atccttttga aaatcggttg tattctgtta attctaggac
    21541 ggcatccacc atttgacgag tcatcaagcg gaaatcacga gcgccatcga ccatttctgt
    21601 atcgctgatt ttattgatca actggtagaa ttttttcgca aagaaactgc gtatcgcagg
    21661 ctcgccttca cgagtcgaac gccgggtgcc gacacaatcg atatcagaag tagtgatccg
    21721 gttgatcatc tcaggaagca gttcgggcgg atcttgcaga tcaacatcca tcacggtcac
    21781 taaatctcct gtagaagctt gcaatccagc gtacagagcc gcttctttcc caaaatttct
    21841 ggaaaatgag acaaagcgga cataaggata agctgaggct agcttcttga ttgctgctaa
    21901 cgtaccgtct tttgatccgt cgtcaataaa caaatattcg aactcatggg cacctttgaa
    21961 tttttcaacc tcatcaaaaa aaagaggaac agttttttct tcattaaaac atgggacaac
    22021 aattgatact ttcatcgttt ctatcctttc aatcgtatgt ttatatatgg tttacattca
    22081 aaatttaata aaccttaaat aacaccttta aaatttgatt ttacgtaaaa gaacaggtat
    22141 tctttttgta aactaacgta tttagtttac catgtttcct cggtttatca aacttttaag
    22201 aacaattttt attagttggc aagtagtttt ggatagagaa aaaggaggaa gttccagtct
    22261 attgactagg agcaaacaaa atgaaaatac acacacctat cggcttttta tggttagatg
    22321 cagatgaatc cggattgact gctgtatctt ttcagccaat ttcagaaagc gaatcagcga
    22381 atcagcgaat tttaacgcgc acacaagaac agtttgaaga atattttgcg ggaaaaagaa
    22441 aagaatttga tgttccttta tctataaaga gaggtacgcc ttttcagcaa aatgtctgga
    22501 aagctttgtc gaccattcct tatggagaaa tccgaacgta taaagaagta gccgttgctg
    22561 ttgatagtcc gaaagctgtg cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa
    22621 tcgttattcc ttgtcatcga gtgatcgggc aaaatggaaa actgacagga tatatgggaa
    22681 acgcaataga tggggttgaa atcaaacgcc aattgttgtt gttagaagga tatctgcaaa
    22741 attaaaaaca gttgtgattc ggacattgtt ccatgcgaga atcacaactg ttttttcatg
    22801 ttcattctaa actttgatca gccaaataaa tagtaaagcc atcactgttt ggagaagccc
    22861 gacagctaat ccataagata agaagcgtgt accttctttc atgaattttg caaagtctaa
    22921 acgcagacca attgccgcta aagcagtgat ctcaaaccag ctgctgataa aatgtgccga
    22981 ttggtcaata actacaggta aagaaaacaa gctgttgacc aaacaaaaga tcacaaaccc
    23041 taatacatac caaggaagcg gtaattttcg ttttttctgc gtatcagttg gcgtttcaaa
    23101 cagagctggt tgtttcttgt gcatgaattt ttcaaacaga tagacaacaa caacgagcat
    23161 cataatacgt agaatcttaa atagcatggc gaattcgaca acagaattgt caagtaagct
    23221 tgccccagcg actacttgac caacagattg gagtgtacca cccaaaagtg cacttttttc
    23281 aatgagattt ccaccaaaca actgtaatcc aagaaaaggg agagtcaaca tcatcacagt
    23341 acccagtaga ttgactagtg tgatgatctg tcctttttct tcctctttcg catggatgga
    23401 aggggcgatt gaagcaatag cagaagaacc acaaacagcg tttcctccag ccatcatcaa
    23461 agaagaagta tcgctgaatt gcagtttttt accaatcaag taagcaccga tgatagtgac
    23521 tgacatttgc agtaacacaa aaacaattcc ttttacacca atttgagcaa tcgtttgagc
    23581 tgtgacagtt gcacctagta aaaaaacaga gaattctaat agtttgcttt cagcaaattt
    23641 tgtgccgatt gctagaacag gctgtttaaa aaaagtattt cctaataaaa tacccaataa
    23701 aatagcaata gtcgctccac ctaactgtgg cagataaata gcaagaagcc tgctgaaggc
    23761 agcgatggca aaagaaagca gtaaaccggg taagatttcg cggaccgctg aatcctttac
    23821 agattttatt ggatgtgaca atgatttcat ttgatcggtt cctttcgtca ataaatacta
    23881 aaagcatact cgaacatagt agcactaaac cagtattttg acaaaagaac aagaggtcgt
    23941 gacaaaagtc ttgtacgacc tcttgtttcg aataaacggt gttccagaag tagcttctac
    24001 ggaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    24061 tcttgaagct gaccacttct tttacgacct cttggtaaac ggtgttcaaa agcttgcatc
    24121 tgcggtaata agcccaacca gtacaaaaac atgaacagct gtttttgttt ggccgttctt
    24181 attacttgat gcagaacagc tttttcacaa cctattgaat cgattattaa gcgatttttt
    24241 tagaaacaaa ttttccatgt ttgctatgtt ctttaattaa gaaacgtacg taatgagcaa
    24301 gaacgtagaa gacaactaca cttgcaaaca gacattccgc aacttttaag aaatccaata
    24361 cgctactata gatacctaaa ctgttgaagt aatgaacagt agtaaatata gctgttgcag
    24421 agataacaag cgggaatgta aaggctgcat agcttggata aaaacgaagc ttgaacaaac
    24481 gtggaagcat agacagcaca acgaaataca atacttgaga taaagcgaac aatgctacga
    24541 caagataaaa attaggttca gcaaagtttt taagataacc tgttaggcaa agagaaccag
    24601 gtgctgcaac gatcgtaatc agcggacgtg tcggatgagc catatttctt acgaaaaaga
    24661 cacgtttgat gatgatcggc aataagaacg catagcagat caatgaaatc caaaagaaaa
    24721 tttttccaat aagtgggaag aagttcccgg cagttacagt gatgacccca aatccaacgt
    24781 aaatgataaa ccagcttgga taaattgctt caattgaaag atcagctttg ataacatggt
    24841 gataattgaa ataaaagaca aggatcactt ggaagataac agcgcacagc caaatgaatt
    24901 tgacaattgg tgcaatactt tcatatgaaa caaaataagt acaaataacc atgagtgaca
    24961 tcgtaaaagt tggtgaaacg gaggcgatga ttggatcatg caacgtttgt ttcgtgtgtt
    25021 cgaataagat aaacaattta acaaaaatga atatcataag aatcatagag ataccgccag
    25081 ttagattgcc taaagcaaca tggtgatagt ctttaaacag atttccaagg gaagctaacc
    25141 caaggatcaa cccgcagatc gggatcggaa taattttcaa aaagttcttc atatataata
    25201 ttctcctttt tatttaatag attttagtac caagatgaac gaatacattg taaaatgaat
    25261 aggaatgtat gattacgagc aagcagacag ctgattgtct tcatctaagt tactagttgg
    25321 atcagcaact gttttgttgt acatttctat tatagatggt tttttgtata tttaaatcaa
    25381 atagttttta tagtaattat cataaaaagt tatggaagtg gtgccatgtt taagttatta
    25441 acgacttttc gagcagtata tgaaacaaga aatttttcca gagcagcaga agtactgttt
    25501 ttatctcaac cagcagtctc gaatcaaatc aaacagttgg agcgggaatt gaatatcgat
    25561 ctttttcaac gtaacggaag aaaagagatg cttccaacta agcaagccga cttattatat
    25621 gaacggacac tggttttgtt agaagaatgg gaagaaactc agcaaaaatt gctcaatgaa
    25681 caaaatgaag aagaagtatg tcggatcgtt gcttcccaca cctttgctgt ctatcttttg
    25741 cctcagttga tgaaacatct gatcaaaagg tttcctaaag tgcattttga gatcgaaatc
    25801 acgaattctc atgaatcatt agaacaagta gcgaaacatg aatccgattt tggttttatc
    25861 gaaaagccat tagagacttc cggcgtacaa agatactctt tgatggaaga ccagctagtc
    25921 atcgctgggg acccaaaaag caaattgtgg cttgtaagag aagcaaactc tggtgttttt
    25981 cattacacgc agcgttattt tgaagagatg aatatccaag ataagaagat gatcgtgaaa
    26041 agtaacgatg taattgttgc gttgctgaag gaaggaatcg gtcgctcgat tttgtctaag
    26101 cgctctgttc cagaaaatgt gccaatgact ccgttgaatt tccatcgata tttttatttc
    26161 atccaacgaa ccaatctggt ttctgatcag ctgctagaga ttgccgaaga gatccttcga
    26221 tacaatgaaa gcacagaaac ttctcacagc gaggagaatc tcagctggta gataattgca
    26281 tgtatcaaaa aaagttctat gatacactaa agataagcag taggcttttg tactgcattt
    26341 gctcaagcaa ctgcacaaac ggaaagttaa tttttcccaa atgtctatgg aaagactttc
    26401 cattatctaa ctggaccgct ataataatca gcagtatggt ttttaagaaa agggatgaaa
    26461 catggaaaaa aaattaaaag cttcgaacga taatcgtatt gattatggcg tgattttacc
    26521 agtctttctg ttatgtttga ttggtctgct ctcattatat gtagcactta gccatgatcc
    26581 gaacaatcca gcagtcttga aaggcgtagg gatgcaagtt gtctggtatt tagttggagc
    26641 ggcagcaatc gttgtcatca tgcatttgaa ttccaaatgg atctggaaac tgactcctta
    26701 cctttatggt ttggggttag tggtaatggg actcttgctc aaattctatg atcagaattt
    26761 agctgcttca acagggtcta aaaactggtt tcgattcggt gcgtttactt tccagccagc
    26821 agagttgatg aagattgcgt acatcttgat gatggcactg atcgtaacta aacataatac
    26881 gcaagtaaaa gaacggacga tgaaatcgga cttttggctg attgggaagc tcttgatcgt
    26941 gacaataccg gttttagcat tgatcatggc ccaagatgac tttggaacga tgcttgtctt
    27001 tcttgcgatc tttggcggga ttttcctgat gtctggaatc tcttggcgca tcatcgcacc
    27061 tgtagtaatc ttggcagtag tcgttggagc aggaacgatc ttcttggtta cgactgaagg
    27121 cggaagagac ttgctttata aagttggatt caaatcttat cagtttgcac gtattgattc
    27181 atggctggac cctttccatg atacgtcagg aatgagttat cagccggccc aaggactttt
    27241 ggcaattggt accggtggct tgtttggtaa aggattcaac gtaagcaata tctatgtgcc
    27301 tgttcgtgag tccgatatga tttttaccgt tatcggtgaa aacttcggct ttatcggtgg
    27361 tgcattcgtc attttcttgt attttatttt aatctatcgt atgattcggg tgtgtttcga
    27421 tacgaataat gaattttacg cctatatcgc aagcggtttg atcatgatgc tgctgttcca
    27481 tgtctttgag aatattggag caaatatcgg attacttcct ttaacaggga tcccattgcc
    27541 tttcattagt cagggaggat cctcgatttt agggaacatg atcggtatcg ggttgatatt
    27601 atcgatgaga taccaaaatg aagcacctgc aagatcttca aggaggtaag aaaattgtac
    27661 accttttact ggtacccgaa atgttcaaca tgcaaaaaag ccaaagcgtg gttagaccag
    27721 catggcgtta gttatcaaac cgttgatatg atcaaagaag taccgaaggc ctcaacgatt
    27781 gagacttggc tagaaacgag tcaagtacca atccgtcgcc tgttcaacac aagcgggatg
    27841 aaatatcggg aacttggact gaaagatcaa gtaggcgagt ttacggcttc aaaagcgagc
    27901 caagtcttat cgacagatgg tatgctgatc aagcgaccac tgatcttacg agaggaccag
    27961 cttgttgcaa ttggattcaa tgaaaaaatt tatgaaggag aactgatata aaatggcagg
    28021 caagcatttg aagaaaaagg acaacctttg gatcttattt aatggaaaag aatactgtgt
    28081 tggtttgaca aatgaagcgc aagaagaact tggagacatc acgtttgcca atctgccaaa
    28141 agcaggacaa tcgctaaaaa aagatgaacc tctgatcgaa gttgaagcgg aaaaagcggt
    28201 caatgaattt gccagcccgc taacgggtac agtttcttct gttaatgata aaatcggtga
    28261 agatatcaat atcttgaatg attcagatga aatgaatgcg tggatcttaa gtttcaaaga
    28321 tgttgatcca aaagaatttg acgaattata aaatcagaaa atcacttgaa aaaagttgac
    28381 actgattctt ccagttgcta taatcataag caatcctata tgaaacgaga aagctttgaa
    28441 aagaagagta catgtcacgg acgtttgaag agagccctgt ttgctgaaaa tgggtaacga
    28501 aaatgagatg gaagatggtc ttagagcaga gtaagcgagc gtaagcaaac ttactacggg
    28561 agtgcccgtt acagcgcgac agtatacgga aagaagctaa gtccaagtac tggaagaggc
    28621 tgttattgcg aagtaacggt aaatcaaggt ggtaacacgg aagttcaagc tttcgtcctt
    28681 ttgcttaagg caagagggcg aaggcttttt tgtttgaaaa attataagag aaagatgtga
    28741 atggcatgtc attaatccaa ctaacgaatg tcaaaaagac attcaatggg aaaaacggaa
    28801 acatccaagc cgtcaatgac gtttcactaa acgttgaaaa aggggatatt tatggaatcg
    28861 tcggctattc tggcgcagga aaaagtacat tagtccgtct cctgaatgga cttgagcttc
    28921 ccacgagcgg cgaagtgatc gtaaacgatc aagatattac aaaattaaaa aacaaagaat
    28981 tacgtacatt ccgaaagaaa atcggtatga tcttccagca tttcaacctg ttatggtcaa
    29041 gaactgtact tgaaaatatc cagctgccgc tggaattagc tggcgtacca aagagcaaac
    29101 gtaaagaacg ggcagaagaa ctgttgcgtt tagtcgggtt agaaggacgc ggacaagcat
    29161 atccttcaca actttccggc ggacagaaac aacgcgtcgg tatcgcacga gcattggcaa
    29221 atgatcctga gatcctgctt tgtgatgagg ctactagcgc attggacccg caaacaacag
    29281 aagaagtatt agacttattg ttagcgatca ataagaaatt gaatttgacc atcgtcttga
    29341 tcacacatga aatgaatgtg atccgcaaaa tctgcaacaa agtcgctgtg atggagttgg
    29401 ggaaagtcgt agaagaagga gacgtattga ctgtcttcag acaaccgaaa aaagaagtaa
    29461 ccaaacgctt tgtacaacaa gacatcgaac cagaagctga ttcgacagaa ctattagctg
    29521 aactcatcaa agaaaatcca agcggccgtt tggcaacttt gacctttagc gaaagaaatg
    29581 ccaacgagcc agtcatttcc caagcgattc gacgctataa catcgatgtc aacgttgtgt
    29641 atggaaagat caaacaagtg aaagaaggtt cctttggttc attgactgtc ttgatgactg
    29701 gtgaaaccaa agaattagat caagcagaag tgttcatcaa agaacaagga gtaggaattg
    29761 aggtgatcca tcgtggataa aagcttttca gaaacatatc tagattttaa acaaatcaac
    29821 ccggaaacga tgaatcaggc tatccttgat acactatata tgacattaat ctcaatggtc
    29881 ttagtgacga tcatcggatt agttttggga ttgatccttt actctattgg acgcaaaaac
    29941 aaaccgtatg ctcgaatcat ttatggaatc gtatcgatca tcagtaatat tttccggtca
    30001 acgccattta tgatcctgat ggttttgatc attccattta ctaaagcaat cgtaggcact
    30061 atgctaggtg cgcaagcagc cattccagct cttgttttat ccgcagcacc attttatgcc
    30121 cgtctagtag agattgcttt tcgtgaagtc agccctggtg tactggaagc agcagatgca
    30181 atgggagcaa gttactggga aatcatctgg aaggtcttga tacctgaaag tattcccgca
    30241 ttgatttctg gattaacagt tacaacgatc tcgatgatcg gatttactgc gatggccggc
    30301 gcgatcggcg caggtggact tggaggtctt gcttggcagg aaggctacca gcgtggtaat
    30361 ctaacagtga cattcgttgc gactttaatt attttactaa tcgtatttat cgttcaagga
    30421 atcggtgatt tcttaactaa gaaaacagac aaaagataat aggatatagg gggatgtagg
    30481 atatgaaaaa gaaaattttt ggatttgcag cagcgctttt gctgactgta ggattagcag
    30541 catgcggtaa caacagcgac agcaaagatt cagcaaataa agcagacgac acgacattga
    30601 aagtcggcgc ttcaccgaca ccgcacgcag agattttgga acatgtgaaa ccattattga
    30661 aagatgaagg aatcgatctt gaaatcgtca aattcgatga ctatgtatta ccgaaccaat
    30721 ctttggaaga aggcgacatt gatgccaatt acttccagca tattccttat ttgaacaaag
    30781 aaatcaaaga aaaaggatat gactttgtca atgccggtgc tgtccacatc gaaccaatgg
    30841 gcttgtactc taaaaaagtc aaagatgttt ccgaattaaa agatggtgca acaatcatta
    30901 cttcaaattc tgaatcagac tggggacgca tcatcacaat tctacaagat gctggcttag
    30961 tcaaagtcaa agatggagta gatctggaaa cagcaacatt cgatgatatt gaagaaaacc
    31021 ctaagaactt gaagttcaac catacgatcg acccatcact tttagcttct acgtaccaaa
    31081 atgatgaagg tgacctagtt gcaatcaacg caaacttcgc ttacggcgca gggttgaatc
    31141 cattagaaga tgccgtattg cttgaaaagg acaactctcc ttacgtgaat attattgcga
    31201 ctcgtaaagg tgatgaagac agtgacaaaa tcaaaaaact gattgaagtg cttcaccgca
    31261 aagaagtcca agaatggatc acagacaaat ggggcggctc tgttaaacca gtaaaggctg
    31321 atgcaaaata aggtatggaa caaaaaagat gagaaataag tctgtaatgg cttatttctc
    31381 atctttttta tacctattct aatttagaaa tcaaaattgt ttaataataa ataaaagata
    31441 tttgatttta gtaaggaagg tcattgcgct tcattctcaa ttagtctaaa atgagtgaga
    31501 acgaaagaga atttgggggt ataatgcatg tctgtcttag aaataaaaaa tctacatgtg
    31561 tcgatcgagg aaaaagaaat ccttaaaggc gtaaacctaa caatgaaaac cggagaaatc
    31621 catgccatca tgggacctaa cggcacagga aaatctacct tatctgccgc tatcatgggc
    31681 aatccaaact atgaagtaac agaaggcgaa attttgtttg acgggaaaaa tgttttagac
    31741 ttggaagtag atgaacgtgc gcgacttggc ttatttttag ctatgcaata tccaagtgag
    31801 ataccaggta tcacgaatgc ggaattcatg cgtgcagcga tcaatgcgaa acgtgatgaa
    31861 aacgacaaaa tttctgttat ggcatttttg aaaaagttag atgaaaaaat ggctttacta
    31921 aatatgccag aagaaatggc tgaacgttat ctgaacgaag gtttttctgg cggagaaaag
    31981 aaacggaatg agatcctgca attgttgatg ttagaaccta catttgcaat tttagacgaa
    32041 atcgattcag gtctggatat cgatgctttg aaagtcgttg ctaaaggagt aaatgaaatg
    32101 cgcggagaca atttcggtgc attgatcatc acccactatc aacgattatt gaactatatc
    32161 acacctgatg tcgttcacat catgatggaa ggccgagtag tcatgacagg gaatgctgac
    32221 ctagctaaac gtctggaagc agaaggctac gctggtatca gtaaagaatt aggaatcgac
    32281 tataaagaag aagaagcgta agggggagca agaatgaaag aaaaaaactg gctcgattat
    32341 cttgacgcgg taaatgattt ttctttaagt aaaggcgaac ctgattggat gagaaccttc
    32401 cgtcaagacg cactagcgaa agcagatgaa ctgccattgc cgcatatcga tcgggtgaaa
    32461 ttccatcgct ggtcgttatt tgatgtgaaa gaaacacaaa ccatcagtga aacaggcacg
    32521 attccggcat ttgatgcaat gaaagacaat cctgtgcttg tccaacaagg ttcatggacg
    32581 atttttgaac aactgcctgt ggaattagca gaaaaaggag tcatttttac cgacttgttc
    32641 actgcaatga tcgaatatcc agaactagtc caagaatatt atatgaaaaa agcagtaaac
    32701 atgaacgaag accaattgac tgcactccat gtagctttta tgaacagcgg tattttcctg
    32761 tatgtgccta aaaatgtcgt gatcgatgaa ccactcgagt ccttattcat tcaagatggc
    32821 gcatccgatg agcacttctt caagcatgtg ttgatcgtgg cagatgaaca tagtgaattt
    32881 tcttatctgg aacgtttcca gacaaccaaa gaacaagtag caaaatcatc aggtaatatc
    32941 atcgtggaag tcatcgctaa agctggttca aaaatcaaat attcagccgt agatcaatta
    33001 ggagaaaata ttacttctta tatgaatcgc cgtgggcaca ttttacgaga tgcttctgtc
    33061 gactgggcaa ttggtgtcat gaatgacggt catgtgatcg ctgactttga ctcagattta
    33121 gcaggagaag gtgcacacgc agaagtcaaa atcgtggcaa tcagctcggg cagacaaatc
    33181 caaggaatcg atacacgagt aaccaataaa gcccctcata cgatcgggca tattttgcaa
    33241 cacggagtga tccgagaaaa aggaacattg acctttaatg gtatcggcca catcttgaaa
    33301 ggggcaaaag gtgcagatgc ccaacaagaa agtcgtgtct tgatgctttc tgataaagca
    33361 cgtggtgatg cgaatcctat tcttttgatc gatgaaaacg aagtaactgc tggacatgct
    33421 gccagtgtag gacgagttga tccagaagaa atgtactatc tgatgagtag aggattgcat
    33481 aaagaagaag cagagcgctt agtgattcga ggattcctcg gttctgtctt aacagcaatc
    33541 cctgtagaac aagttcggaa agaattagtc gaagtaatcg aagggaagtt gaatggatga
    33601 tcgatgctaa aaagctacgc aatgattttc ctatcttatt tcaagacgtc aatgatgaac
    33661 ctttagttta tctggataat gcagcgacga cccaaaagcc aaaacaagta ctccaggcat
    33721 tagcgtacta ttacgaacac gataacgcca atgttcatcg cggtgtccat acgttagcag
    33781 aacgcgcgac ccatgattat gaacaagcaa gagaaaaagt acgggcgttc atccatgcaa
    33841 aagaaacagc agaagtgctg tttactcgtg gtacgacgac aagcttgaat tgggtagcca
    33901 gaaccttcgg cgaccgctat atcaaagcag gagacgagat agtgctctct tacatggaac
    33961 atcattctaa tgttatcccg tggcagcagt tggcaaaacg aacaggtgct gtcttgaaat
    34021 atatcgaact aacagaagaa ggcttcctgg atatggagga tgcagcgaaa aagattacgg
    34081 acaaaaccaa aatcgtctcc attgctcatg tatccaatgt cttaggtgtc ttcaatcctg
    34141 ttcaagaatt agctgaactt gtccatgaaa acggcggggt catcgtagta gatggtgctc
    34201 aatctgcccc gcacatggcc attgatgtcc aagcactaga ctgtgatttc tttgctttca
    34261 gcggacacaa aatgtgcgga ccaacaggta ttggtgtttt atatggtaaa cgaaaatggt
    34321 tagaagaaat ggaacctgtt gaatttggtg gcgaaatgat cgattttgtc gaattatacg
    34381 acagtacatg gaaagaacta ccttggaaat tcgaagcagg gacaccaaat atcgccggcg
    34441 ctatcgcatt aggccatgca atcgattatt tgcaaaaact cggtatggac aatatccatc
    34501 gatatgaaga agaactagcc gcttatgtgt tgccaaaatt acaagcaatt gatggattga
    34561 ctgtttatgg tccacaagac ccgaattttc ggacaggagt cgttgcattc aatttggatg
    34621 ggttacatcc ccatgatgtc gcgacagctt tagacatgga aggggtagcc gtcagagcag
    34681 gtcatcattg tgcgcaaccg ctattgaagt accttgaagt atcagcgact gctcgcgcga
    34741 gcttttattt ctacaacaca acgcaagacg ccgaccgttt agttgaagca atcatggcaa
    34801 caaaggagtt tttccaacat gggactatct aaattagaca atttataccg tcaagtgatc
    34861 ttggatcatt cttcccatcc gcatcatcgt ggaagcttga ctgactcgac aagcaagatc
    34921 gaattgaaca accccacatg tggagatgtg atcgaattgc aagtcgatgt aaaagacgga
    34981 atcatcaaaa atatcgcctt tgacggcagt ggctgctcca tcagtacggc aagtgcctcg
    35041 atgatgacag acgccgtcat cggaaaatca atagaagaag cagaacattt aactgtagac
    35101 tttctacagc ttgtccaagg aaaagaagtc aaagatcccg accaattagg cgatgctgct
    35161 atgcttggtg gtgtagctaa atttcccgct cggatcaaat gtgcgacact tgcctggaaa
    35221 gcactaggaa aagcaatgga aaacgatggt accgcagctt tccatcatta ccacaaggaa
    35281 gaggagtgag aacatgggcg taccagaatt agaagaatat aaattcgggt ttcacgatga
    35341 tgtcgaaccg gttttcagta caggagaagg cttgacagaa gaagtgatcc gtgagatgtc
    35401 aagaatcaaa ggcgaaccag aatggatgct tgaattccgt ctgaaatctc tagaaacatt
    35461 taacaaaatg ccaatgcaaa catggggccc cgacctttca gacatcgatt tcgatgccat
    35521 caaatattac caaaaaccaa gtgacaaacc cgctcgtgac tgggaagacg taccagaaaa
    35581 aatcaaagat acatttgaaa agattggtat accagaagct gaacgagctt atctagccgg
    35641 tgcttccgca caatatgaat cagaagtcgt gtaccataat atgaaagatg aattcgaaaa
    35701 gttaggaatc gtctttactg acactgactc tgctttgaaa gaataccctg aattattcaa
    35761 agagtacttt tcaaaacttg taccgccaac cgacaacaaa ttagcagcgc tgaattcagc
    35821 cgtatggtca ggtgggacct ttatttacgt gccaaaaggc gtcaaagtcg atgttccatt
    35881 acaaacgtac ttccggatca atgcggaaaa cacggggcaa ttcgaacgta cgctgatcat
    35941 tgttgacgaa ggagcaagca tccactatgt cgaaggttgt acggcaccta cttattcaag
    36001 taacagcctg catgccgcca tcgtagaaat ctttacaaga aaagatgcgt attgccgtta
    36061 cacaacgatc caaaactggt cagacaatgt gtacaactta gtaaccaaac gagcaaaagc
    36121 atacgaaggc gcaaccgttg aatggatcga cggaaaccta ggtgcaaaaa caacgatgaa
    36181 atacccaagt gtttacttag atggaaaagg tgcacgaggc accatgctct ccatcgcctt
    36241 tgcaggagca aaccaaatcc aagatacggg tgcaaaaatg atccacaatg caccaaatac
    36301 atctagctct atcgtctcta aatcaatttc aaaagacggc ggagaagtaa actatcgagg
    36361 acaagtaacc tttgccaaaa acagtgcggg ttcgatctct catatcgaat gtgacacgat
    36421 catcatggac gaatggtcga aatccgatac gatcccattc aatgagatcc acaacagcca
    36481 agtctcttta gaacacgaag ccaaagtctc aaaaatctca gaagaacaac tctactatct
    36541 gatgagccga ggcctgaccg aatctgaagc aactgaaatg atcgtcatgg gctttgttga
    36601 accattcacc aaagaacttc caatggaata tgcagtcgaa ctcaatcgat tgatcagtta
    36661 tgagatggaa ggcagtgttg ggtaaacctg ttataggagc tagaaccctt gatatataag
    36721 ggttctagct tttttgtttt gcctgtatac tagaaatgac cagattttga ccagctcttt
    36781 tactgatgag ccatcatatc agcaaagtct tgacctgttt tcttaggtgc attatttgta
    36841 atatgtgcat agatgtctaa tgtcacttta ctattttcat gccctaatcg ttgttgaact
    36901 accttgacag attctcctgc ttctaataat aaactagcat gtgtatgtct aaacccatga
    36961 ggtgtaatac gaggcaagtt atattttttc aaaatatatc ttaaccaatc attaacagtg
    37021 tttggaatgt atagagtgtt tttaactgtt gtaaaaacat gttgctcttt agagctagta
    37081 ttgtatccaa gttttaaata ctcttctttt tgctgtaatt tccagttgtt gaggatagaa
    37141 agagtctcag tatctaaaga gataactcgt ctagaagctc ttgttttcgg agattgaatt
    37201 ataactttac caaattcatc tacagcaaga gttttattga catgtagttc tttattaaac
    37261 gtgtctatgt ctttccactg taaagcgagt acttcactct ttctcatacc tgtgtatgcc
    37321 aataatctga agaaggcgta ttgtttcatg ttaccaaaat ctttaaaagc atccagtaaa
    37381 ttattaagct cctcttttga gtaaaattta cgatttttct catactcttt ttttcgtgga
    37441 agaagtgttt tttccatagg attattgtca atatattgca ttgcaacccc gtagcgtaaa
    37501 atttgtgcag tctgttttct catataatcg taactttcat atttactgtg ccattcatct
    37561 accacttttt gacaatatgg gacagagata ttcgatagtt taagcttacc aaatgctgga
    37621 agtatttgat tatttgcgta tcttactgag attgcgattg ttgaagcttt aacactggta
    37681 cgctgatgag ttatccattc attatacagg tcttcaaatg tcatatcagg tgctttcttt
    37741 ttaacaccgt ataatatttg atgtctgtac tcgtcataat ctttttgagc ttcttgtttg
    37801 ctactaaaac ctcttctcgt agtatttatt ttttcaccag ttcgtggatc ggtggcaatg
    37861 tgtcctttat acatccacgc cacttgaccg tttttctttt tgtactttga tatttttgcc
    37921 atgaaatcat ctcctcttag tttacaagga gtacttacca ctagatggta tcacctccct
    37981 tcattttttt cttagtgtat tttttcccaa ttgatgaaat cgtttgttct agcttgttta
    38041 tattatcagt aatttctctc acagcttctt cgggataaga catgtctttc aattgaatag
    38101 gtagccattc atctaccatt ctttctagta gttcttctat actgctagtg aggaaaagaa
    38161 tgttattgtc aaattcttct acaatagcat tgatgaataa ttcttctatt tttaagatat
    38221 tgaaaagact aattttttca tttttaactt tattacaagt cttgttagca ataaattctt
    38281 gcatctctgg ttctaatttg gaatatctat ccagccatgt atctaaatta gagtgtactg
    38341 gtgggaactg ttgacgaaga tgctggaaca ggtttttatc tggaaatgtt ataccatcta
    38401 gtacaatgct atctctataa agaataattc ccaaaatata ttcttctaca ttgccacaaa
    38461 ctaactcctc tatactcata tttcccaaat cagcaattcg ggataatcgc tctggtattg
    38521 gtaaataggt tcccttctcc cattcgtaga tagttttttt gtcaattaat tgaccgttgt
    38581 taatcataag accaaatgct ggttgtgtta attttttact ctttcttata gcttttattt
    38641 ttgttccgag tatctttttt cgttctaaat aatttattgg tggacgtttt atcatattga
    38701 ccacacctcc taaaattagt atacctcgaa aaaggggtgt tgtaaataaa ttttacaaag
    38761 atattgacac ctctttttta tcgaggtatt atgtgtatgt cgatgaactg gtaatcattt
    38821 tttgtttgat taatcggtta ataacaaaat acttttattt tgagcaaaaa atacgaagat
    38881 aattcttaga aaatttgttc aagcaatcac gaaaggaggg ggaaatatgt ctctacaaat
    38941 cattgatagc attgtttcta tacaagttga acaacaagtg aaagagataa ttacagtgct
    39001 aaagcctatg ctagaagaca ttatttctgg agtcactcta gagtatatgg actatcagca
    39061 ggcaagtgaa tatctaggtg tatcagtagg aacgattaga aaatatgttt ctcaatacgg
    39121 gttacctgtt ataaaaattg atacggtcat tagatttaaa aaatctgata ttgatagttt
    39181 catggaacgg tataaaggtt gagtaaacaa ataagactaa aacatatagt aataaagagt
    39241 ttgtttttat tatctgtatg ttttagtctt taacagaatt ttataaggag gaaacacaaa
    39301 tggaaacatc aatgattaat attccaattt atgtagataa agaaaaatta ttagagaagc
    39361 cagttacaaa aaaagaaaaa actgatgtaa gagataggtg tttagataac tatagagaag
    39421 ttacaagaga tgagtttgta gagatactaa attcatcaca atcatttatt ccaagcaaga
    39481 caaagaataa aggcaatgac aaggagagtt ttgtggaaac cagagtaatt attctggatg
    39541 tcgataacac tgttaaagat gagaagggga aggtagtaga tttgagtaaa gatgatagta
    39601 gatacttaag catagaaaag gttttatcta tcaatttagt tcataattct gcgtttgcga
    39661 tacaaaaaag tattcggtac tctgaaaatc tagaaaaata caagattgtc tttctattaa
    39721 aagaagcaat tactgactat ggtaaaatgg ttgctgttta cgaatacttg aaaaagaaca
    39781 tccccgggtg tgatgacaat gtaaatgctt cgaatagaat gttttttgga ggatataagt
    39841 cagatgcagt gataataatt aatgaagaca acatgttaga tattactgag cttccgattg
    39901 attttaccat gacatgttct aaaaattgtc aaagtgattc agggagagag tatgaactag
    39961 tcaatgaaac cgattttgtt aagttgataa aaaatgacga caaagaagaa atgaaacagt
    40021 ggtttaaaga ctgcctgttt gattcctccg acatgtcttt taatgagatt tatgaaagat
    40081 tgttaaccat tgatatgaat aaattactta agtcaagtaa aaacttaaga tgtctgtttc
    40141 acaacgatca taatccctct gctagtattt ttaccagtaa aactgggcat tccatttttt
    40201 attgtcattc cagtaattgt gatatcagtg aaaattttat tggagttgtg atgctaattt
    40261 taaagaaaga cagtcgggta gaaacattta attggctatt agagaaccta gacttgtatc
    40321 ctacacattt tcgaaaattg aaagaggaat caaaagtact ttttgacact cttgaatcaa
    40381 tgaaagacaa acttttcagg acaataagga attatctaga ggaaatgcga cgtgtctatg
    40441 atgtcttgct ttctgatgtt aatttttttg atgatagtaa ggaaagtgtg acttgtattt
    40501 tgtcggggga acaattagct aaaaaaatga gtttttacta tggtaaaact tatgacatag
    40561 ataaatgtaa taagttacta tcacttatga cttttatagg attgataaaa aaattagatg
    40621 atgaagatgt tcctttgaag atgctcgaat acctaaatag gttaaaggaa gagaaagcgt
    40681 taggaaactt taatcataag agaagcaatg tctatcgatt agacgttttt aatgcaactt
    40741 ctgtgattca aaaattgaat gaagatatcc ttccacttct ggaaaaattt cattttacat
    40801 atcaatattt ttcttatgaa tgggttaaga tatgttttaa cgaaaaagag gctaaaagag
    40861 tatttcctca aaattcccga tcagaactta gcaaggaaaa acaattgatt ttagatgaag
    40921 cacatgaagt tttacagggc ttatcgtggc aaggaacata tctaatgaca gagaaagaac
    40981 ttactaacac tgtttgtgaa cgtctacgta atgttgggaa taacagcgtg gaaaatgctt
    41041 taaaagaaaa cagaggctac tttgttaaac aaggattcac tcttgaacga gcttctaagg
    41101 atattaagag actatacaat gtgaaagcaa gtagtagttt tttagtctac ctagcaaaag
    41161 gagcagggat agatgaagta ataaaagaat tgatattatc aggagttgaa ttagttcctc
    41221 tttatcaatt tacaagtgta acaagagatg atggacggag agtaaggaag agaaaagacc
    41281 caattgtatt aaactagaca ggttatccat agctcttaat cttatttcat ctcaatacac
    41341 ccaccgttaa agttcggtgg gttatttatt tttcaatctt tcaatagctg attgcgttca
    41401 ttttcttgcc tttttaatag cttcatatag tcaattgttc gaactgcatc ttcagtgcta
    41461 agagaacgat agagagccaa aaactcttcc tcataatcta cgacaacatt gtctagaaaa
    41521 tggcttacag gggttcctag ggctctagaa agagcggtta agctatcgag gttaggctct
    41581 gttacagaat tttcccatct tgctactgcc tgttgtgtag agaagatttt ttcagctaat
    41641 tctttttgtg tccacccgag agcttttcgc ttttctctaa tgacctttgc atattgtttc
    41701 tgtttcatta atacaccccc atctcagtat agtacaacaa gttgttgtat ttgtaatggt
    41761 atttgaagtt tttttatatt ttatgagttg aaatacatca tattgttgta tataataaaa
    41821 caggagggga gaatcatgaa aaaagtaagt aatctcagcc gatacagaaa agaaaaagga
    41881 ctttctcaaa cggagttagc aaaaaaatga atgttacaca gcaatgtgta agttcgtggc
    41941 aaacagggag aacgattcct aaaccctatc aaatgaagat gctttctgag atattaagtg
    42001 taccaatgaa tgagctgttt tctgatgtat ttaataaagt aaacagttaa ctaataaaca
    42061 attgaatgtt tttaggagga gaagacatgt attgtagaaa atgtggcaaa gaaataacag
    42121 atgaaagtaa gtattgccag ttttgcggag cactacaaga aacagatgag acaactggag
    42181 cagacgcagt aaagaaaagt actgagcaga caattaatga aatgaaggaa caaatcgtac
    42241 atggacaaga gacagggaag ctgtatttga ttattggttg gatttctatg gtagtagcgt
    42301 tagcatttat tcctgttgta tttggtgctg ttgctgttat tatgggctac ttatatcgag
    42361 ataaagatga acaaatgggt acgattttga tgatagcagg ggtagctgga gcaatctttg
    42421 gtgtactttt agggatgtca atgtactgat aagagggata agagcaaatt attttggaga
    42481 taggagataa aaagttgaat aaagtaaata tgaacaagaa gtttatcatt atagttatcg
    42541 ttgtcttggc tattataggt atcatttttt caaaaatgac aggtacatct ggattagaga
    42601 agtcatcgaa accattggta acacagattt tacaagaaca gtacaactta aatcgatcat
    42661 gtgatgatgt gacgattact catgaaaatg gagataacac ataccgggct aaagctatat
    42721 tagacaatgg aagtgctatc aacataaata ttgagtacta tccaaagaaa gaccgtatat
    42781 atgtggagat accatatgct gaggtgttaa tgttaaattg atttaaatgt attatgcata
    42841 ccgtccaaac aggaactccc aaaggtgtaa attcactagg gagtttttat agtttcctat
    42901 aaattaaata ctaatacagg tgacaaaaaa atattacaat gattgtcggg aatttttatg
    42961 ttattcactg ttataacaat ataaaagaaa tgaaaaagaa aaaattatta tggatatgtt
    43021 tggtattttt a
//
LOCUS       DAYYAO010000016        40440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000016 DAYYAO010000000
VERSION     DAYYAO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.017"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..2691
                     /gene="clpB"
                     /locus_tag="ACHARI_000771"
     CDS             82..2691
                     /gene="clpB"
                     /locus_tag="ACHARI_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8441841.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2842..3528)
                     /locus_tag="ACHARI_000772"
     CDS             complement(2842..3528)
                     /locus_tag="ACHARI_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441842.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFK
                     SYKLEKVKNT"
     gene            complement(3506..4000)
                     /locus_tag="ACHARI_000773"
     CDS             complement(3506..4000)
                     /locus_tag="ACHARI_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8441843.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4020..5240)
                     /locus_tag="ACHARI_000774"
     CDS             complement(4020..5240)
                     /locus_tag="ACHARI_000774"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8441844.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5387..7222
                     /gene="lepA"
                     /locus_tag="ACHARI_000775"
     CDS             5387..7222
                     /gene="lepA"
                     /locus_tag="ACHARI_000775"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8441845.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7418..8329
                     /locus_tag="ACHARI_000776"
     CDS             7418..8329
                     /locus_tag="ACHARI_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441846.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9340..9576
                     /locus_tag="ACHARI_000777"
     CDS             9340..9576
                     /locus_tag="ACHARI_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441847.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9689..9931
                     /locus_tag="ACHARI_000778"
                     /pseudo
     CDS             9689..9931
                     /locus_tag="ACHARI_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10021..10260
                     /locus_tag="ACHARI_000779"
     CDS             10021..10260
                     /locus_tag="ACHARI_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441848.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10407..14690
                     /locus_tag="ACHARI_000780"
     CDS             10407..14690
                     /locus_tag="ACHARI_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8441849.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14789..15154
                     /locus_tag="ACHARI_000781"
     CDS             14789..15154
                     /locus_tag="ACHARI_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441850.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15156..15608
                     /locus_tag="ACHARI_000782"
     CDS             15156..15608
                     /locus_tag="ACHARI_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441851.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16057..17532
                     /locus_tag="ACHARI_000783"
     CDS             16057..17532
                     /locus_tag="ACHARI_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8441852.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17555..17866
                     /locus_tag="ACHARI_000784"
     CDS             17555..17866
                     /locus_tag="ACHARI_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441853.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18227..19483
                     /locus_tag="ACHARI_000785"
     CDS             18227..19483
                     /locus_tag="ACHARI_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8441854.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19480..20025
                     /locus_tag="ACHARI_000786"
     CDS             19480..20025
                     /locus_tag="ACHARI_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441855.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20026..20526
                     /locus_tag="ACHARI_000787"
     CDS             20026..20526
                     /locus_tag="ACHARI_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441856.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20885..21163
                     /locus_tag="ACHARI_000788"
     CDS             20885..21163
                     /locus_tag="ACHARI_000788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441857.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            21183..21527
                     /locus_tag="ACHARI_000789"
     CDS             21183..21527
                     /locus_tag="ACHARI_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441858.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21546..22457
                     /locus_tag="ACHARI_000790"
     CDS             21546..22457
                     /locus_tag="ACHARI_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8441859.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22536..23528
                     /locus_tag="ACHARI_000791"
     CDS             22536..23528
                     /locus_tag="ACHARI_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8441860.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23525..23743
                     /locus_tag="ACHARI_000792"
     CDS             23525..23743
                     /locus_tag="ACHARI_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8441861.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            23756..24166
                     /locus_tag="ACHARI_000793"
     CDS             23756..24166
                     /locus_tag="ACHARI_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8441862.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            24218..26746
                     /locus_tag="ACHARI_000794"
     CDS             24218..26746
                     /locus_tag="ACHARI_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8441863.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            26762..28972
                     /locus_tag="ACHARI_000795"
     CDS             26762..28972
                     /locus_tag="ACHARI_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8441864.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            28980..30071
                     /locus_tag="ACHARI_000796"
     CDS             28980..30071
                     /locus_tag="ACHARI_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8441865.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            30092..30634
                     /locus_tag="ACHARI_000797"
     CDS             30092..30634
                     /locus_tag="ACHARI_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8441866.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            30829..31086
                     /locus_tag="ACHARI_000798"
     CDS             30829..31086
                     /locus_tag="ACHARI_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441867.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            31100..31432
                     /locus_tag="ACHARI_000799"
     CDS             31100..31432
                     /locus_tag="ACHARI_000799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441868.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            31477..32025
                     /locus_tag="ACHARI_000800"
     CDS             31477..32025
                     /locus_tag="ACHARI_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8441869.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            32032..32484
                     /locus_tag="ACHARI_000801"
     CDS             32032..32484
                     /locus_tag="ACHARI_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441870.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            32581..32922
                     /locus_tag="ACHARI_000802"
     CDS             32581..32922
                     /locus_tag="ACHARI_000802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441871.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            33290..33481
                     /locus_tag="ACHARI_000803"
                     /pseudo
     CDS             33290..33481
                     /locus_tag="ACHARI_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(33641..34312)
                     /locus_tag="ACHARI_000804"
     CDS             complement(33641..34312)
                     /locus_tag="ACHARI_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8441872.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            34616..36247
                     /locus_tag="ACHARI_000805"
     CDS             34616..36247
                     /locus_tag="ACHARI_000805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8441873.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            36250..37329
                     /gene="uxuA"
                     /locus_tag="ACHARI_000806"
     CDS             36250..37329
                     /gene="uxuA"
                     /locus_tag="ACHARI_000806"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8441874.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            37403..38830
                     /gene="uxaC"
                     /locus_tag="ACHARI_000807"
     CDS             37403..38830
                     /gene="uxaC"
                     /locus_tag="ACHARI_000807"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8441875.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            38914..39561
                     /locus_tag="ACHARI_000808"
     CDS             38914..39561
                     /locus_tag="ACHARI_000808"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8441876.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            39681..>40440
                     /locus_tag="ACHARI_000809"
     CDS             39681..>40440
                     /locus_tag="ACHARI_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8441877.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLA"
BASE COUNT        13848 a         6080 c         8445 g        12067 t
ORIGIN      
        1 aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg atcttacctt
       61 taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct tcaagaagcg
      121 attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga cattgcccat
      181 gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac ggatgccgga
      241 ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta tccagttgtt
      301 tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg attattgaat
      361 gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga agtcgttatt
      421 ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa aaacggatta
      481 tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag agtgacctca
      541 caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt agtacagcaa
      601 gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc
      661 cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc cggtgtaggg
      721 aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat
      781 ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg agcaaaattc
      841 cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga
      901 cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa aacagaagga
      961 agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt acatttgatc
     1021 ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt
     1081 cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag tattctgcgt
     1141 ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa cgcattagta
     1201 gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga caaagcaatc
     1261 gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat gccaacggaa
     1321 ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa
     1381 gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt agctgaactt
     1441 cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga agtcaattct
     1501 gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga tgcagaaaat
     1561 aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca attagaaaaa
     1621 gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc
     1681 gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa
     1741 cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca taaacgagtg
     1801 attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc acgggctgga
     1861 ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac tggtgtcgga
     1921 aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga tcatatggta
     1981 cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt cggagctcct
     2041 ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag acgaaatcct
     2101 tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg
     2161 ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt agatttcaaa
     2221 aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga aggcgtgact
     2281 gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt acgtggaaac
     2341 ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc attgagccta
     2401 gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg tctggaacat
     2461 caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga aaatgcttat
     2521 gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt agaaacacca
     2581 ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac gattacttta
     2641 cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta atcttccatt
     2701 gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct tttttcaaat
     2761 aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg aagctgcccg
     2821 cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat aacttttaaa
     2881 gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg cagcacctat
     2941 cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga caaacagtgt
     3001 gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc ataagaattc
     3061 ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata agaggaacaa
     3121 ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca atccatagcc
     3181 aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa aagagatgat
     3241 actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa
     3301 caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt gtggcatgaa
     3361 aatcaatgca atccctaata aagattccac gataaatgtg attccttgag aagtcgaata
     3421 tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa aagcaagtaa
     3481 agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct aaaaatcttt
     3541 ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat ttgatcaaac
     3601 ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt agtgcagcga
     3661 tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg atcactggga
     3721 tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc gtcagcccat
     3781 ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca agagcaatca
     3841 ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga ggaagataga
     3901 tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta tataacaaat
     3961 cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca tcctttttat
     4021 caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag tgacttcagt
     4081 gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg gcgataattc
     4141 atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat aggttcattt
     4201 aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt attccaagga
     4261 gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt
     4321 tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat ctgctctttg
     4381 atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc tccatgtacc
     4441 cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg agcttcttga
     4501 tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta gaacagaaca
     4561 cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc ttcattttcg
     4621 aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg gttttgttgg
     4681 gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata
     4741 ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga aagtttatca
     4801 gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata caaataagag
     4861 atccattgct tattcttgcg catattatcc aatgcaacga taattagatc ttgcatcaat
     4921 ccatggttag catattctgg ataagttcca actccagtca caccacccat tttataacgt
     4981 gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag tttattttcg
     5041 tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg gataaaagct
     5101 cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa cacataggaa
     5161 agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc aagattttct
     5221 ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac agtatagcaa
     5281 aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat tactgctttt
     5341 cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg atataaagaa
     5401 aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata tcgaccatgg
     5461 gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt ctcgcgaaat
     5521 gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca cgattaaatt
     5581 aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct ttcacttgat
     5641 tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg ctgcctgtga
     5701 gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat tggccaatgt
     5761 ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa tcgacctacc
     5821 cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag
     5881 tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc tcgagcagat
     5941 cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag ctttgatctt
     6001 tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca cggacggtat
     6061 ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg atgttacaga
     6121 ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg
     6181 ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata cagtaacgtt
     6241 agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc cgatggttta
     6301 ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag ctttagaaaa
     6361 actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag cattaggttt
     6421 tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag aacgcttgga
     6481 acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc atgttaataa
     6541 aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac ctgtaacgat
     6601 tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg
     6661 tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg attatttaga
     6721 tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct ttgacttttt
     6781 cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa tgtctggtta
     6841 tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag tcgatgcatt
     6901 aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg ttgaaaaact
     6961 gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta ttggacaaaa
     7021 aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg
     7081 tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga agaagcggat
     7141 gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc tgaaaatgga
     7201 tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa taaacctttt
     7261 tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc atttggttat
     7321 cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt tagctgtcag
     7381 tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt caagtggaag
     7441 ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag ctgatttagc
     7501 aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa tccctaaaga
     7561 cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag atgaggaaga
     7621 taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata agttacttaa
     7681 caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg tgttaaatag
     7741 aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat taagtaatga
     7801 aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat caaaagacga
     7861 taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag agacgcttga
     7921 aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta gatgggaaaa
     7981 aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg gtaaggtcac
     8041 tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag atgaaagagt
     8101 tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga aaattaggaa
     8161 agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa tttcaaaatg
     8221 gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa ttatccaata
     8281 tgcttatata ggaaatcttt ctttagaatt tttgatttac gggatataaa aatagtttac
     8341 gtcgatactt attaagttat cgacgttttt tattatagaa aaaatttata aaaggaggtt
     8401 tcttgatatg ataagaaaca aaaagggttt gttgaaagtt gctttactgg cagctttttt
     8461 ctaagtaact ttttgacaag ctcaatggca acacaagcgg aagaagtaat acaagaacta
     8521 actgttatga ataacagcta gataaaatgg gctatgtatg ttatccaaca atttagtaat
     8581 cactagaagc ctagcgaaag ttcgcttgat aagttataga tgagtattga tttgtagtgt
     8641 ttccaaatca aaagggtaca gagccacatg aaatatgttg tcctctgcag cgtcaggatg
     8701 ggaatccata ccatggagat tcggagatct gtgagggaat acttacgtgg ttaatatgtc
     8761 agttgtgttg gacgtagtaa tggtcatagt gggtagattg actgaattct tatatgagaa
     8821 aatgaaacca acaagaagat agggttattt atacagaatt ggggatcttt tctggaaaag
     8881 taatcggata atgcgagatt cgtacggtct tttttaaatt tctctaaaaa aaacggcatc
     8941 gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata aaaaatgatt
     9001 ctatttatct ttttacatct atcgtttttt caagtagccg caaattatat cagcattcaa
     9061 caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt gttgtgaaga
     9121 taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt aggttaggac
     9181 aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag taatttccga
     9241 ttattggagt ttttttattt ataaagaaga agcgaataag ttgtgattcc tagatttcgc
     9301 gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac acgaggacaa
     9361 agtttcttag gtattgaagg cacacgtgta acggttgatg ggtggtgtga ttgtttcgag
     9421 gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa caacgtggaa
     9481 tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa atttattttg
     9541 cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta gagatcattg
     9601 ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag gtgatagaat
     9661 gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga ttcttagaaa
     9721 aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg taggtgtcta
     9781 tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag atgcctattt
     9841 acaaaatagg tatcgagtag atggttttaa gcaagacttt gcggagtggt ttgaatggga
     9901 aattgaaaac ggagaagtaa aagaagaata agagtacttg ttgtatcaaa ataagccagt
     9961 agaacaacca aaccagtagc tagtaatgac aataatactg aggaatcgga tcattatggt
    10021 atgattgata tttctcttcc agaagatctt aagccagatc ctgtaacaat tggcaatagt
    10081 gtcatgattt ttgattcatt agatgaagct atgtcatatg gttggaaaga aattaaggat
    10141 gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa aaaagctggt
    10201 attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt tgttctgtaa
    10261 ttatagtggg agggtaaaat cattgatgaa atgtgttcag ataagagcgc cagataaaaa
    10321 tagaagagac agttcacgtc gttatcactt ttggtaacgg cgttttgttt tattatataa
    10381 aattctcagg aaaaggagga attcttgtgt atgcaaaaac gaaaatgaat tggttgaaag
    10441 cactcgcatt ggtggctttt tctcaattct gtttcttggt gggaactgct caagcacatg
    10501 cagaagaaac gacggctata caacctacga tggaagccat tgcccaagtc gcttctgaag
    10561 tgacagaagt ccaaccagca gaagccgggg tcccagctgg tgaagccact gttgaacagg
    10621 cccctgtgac agaagcgcca acggtcaatt ccaaaacgac tctcgatcag acgattgggg
    10681 aaacacaaca cccagggaat agtcctggta cttcacaacc agttgaagaa actgcctcaa
    10741 ttacaacccc tgcttcagaa acgtccactc tagaaggaga aaaagctgcc gaagcaacgc
    10801 cttctgtgca gccagagtct tctgagaaac aagcggaaga aaagaaacga atagaagaaa
    10861 cagtggaaca accagcggct actgctgaca aagaaggaaa aaatgaggaa aacgttgaaa
    10921 aaacaatctc ttcaaccgca gctagtcaaa atcaaccaac gaaaccaaac aaagaatcta
    10981 cggctagtat tgaaaatcaa aagaaaaaag agcagaacat tccaaaaaca ggtacaccta
    11041 cactgttagt aacagtacca accaatcgtt cggttcgtgt tcgccgtgca accaatgcag
    11101 ctgttttagg tggtcggaaa attgattggg gcgaaaatat tggtgttact tctagtccga
    11161 acaatctaaa cttcacttta aattatatgg ttcgttttga gattaatgga caagtggcgt
    11221 tttgtattga gcctggtgtc tcagccgcag aaggtcaagt ttatacagct actgctttga
    11281 aaaactattt gaaaaattca aatgtacgga aaaaaacaag cttgatctct tatttaggat
    11341 atgtcacaaa taaagacaaa tccgatgaac aatacattgc ggcacaattt atgatttggg
    11401 aagctttggg cacaaaaatc ccaatcaaaa atatccgtgt tgattatgat cgtcgaaaag
    11461 cagaaattca acgtttgatt aataactttg gtgaaaaagc aacttttaat aatcaaacac
    11521 atacggtaaa aattggcgaa acgttgaaag taaagaataa aaatgatttt ttgaaatatg
    11581 tgaaagacat tcaaaaacca aaaggtgtca atgtatctat tcacggcaat gaagtatgga
    11641 ttacagtcac aaaagaggca ccggataaag caaaaattca attgaataaa attaaagttc
    11701 aaggtgcgcc attagcctat cgtcgtccag gagctcaaac aattggtgtg ttacatccaa
    11761 cagaacctaa tgcgtctgtt attaatttac aaattctaaa acaaggaaac attcgtgtgt
    11821 ttaaagaaga tgcggaaact ggtgtgaaag cacaaggtgc agcttcttta gaaaatgcgg
    11881 tctatggttt gtatcaagca gatgggaaaa agattaaaga aattaccttg aaaaaaatta
    11941 acggcaaagt acaggcagaa attaaagatc ttgatcctgg caaatatgtt ttaaaagaaa
    12001 tcaaagcgcc aaaaggttat gtgctttctg atgaagcaat tgaaattgtt gttgaacctg
    12061 gaaaaactgt caatgcgacc gcaaaagatc aagtgatcaa aggaaccatt gatttagtga
    12121 aagttgctaa taaagattta gtcgattcaa cgaatccaga taacaaacca aaattagctg
    12181 gcattgaaat cactttgacg tcaaaaacaa ctggcaaagt ggtgaaaacc gttgtgacag
    12241 acaatgatgg ttatgctact tttggtaaaa ataccgttgt ctttgatacg tacattcttt
    12301 ctgaaaccaa aggaaaagaa gggtataaac tatttgaacc acttgaagtc acaatttctg
    12361 aaaatgggca aactttccat tatgttttag aagataaagt aattgaacaa cgtcttaaag
    12421 tggtgaaagt ggatcaagaa acaggaaaag tgattccact tgctaataca gaattcaaga
    12481 tttttgatac tttagccaat cgttatgtca caatggagat tccaaatgat acagaaacaa
    12541 cggatatctt taaaaccaat gaaaaaggct ttttcctaac taacggtaca cttacttatg
    12601 gaaaagatcg ctatcgtttg gaagaaatca aagcccccgt aaattacgtt ttgaatcaaa
    12661 acccattagt gttctcgatt accaatgata cagaaccgat caaagtgatc cattttgcga
    12721 accgtcaagc acggaaaagt gtaaaattat tcaagtatga agaatggaac aagaagaaaa
    12781 cgccgctcgc aggtgtgaac tttaccttgt ataacggtaa aaatcaagca attggtgaat
    12841 ataccactga tcaaaacggg gaaattttag tgaaaggtct agttgccgat gactattact
    12901 tcttggaaaa tgccccatta gaaagtttcc aaccaaatac agataaacaa gcattcacgg
    12961 ttgcattcaa acaaaatgcg ccagatgatg gggaaacgtt agtggtttct gtacgtaatt
    13021 acttgattcc accaaaacta gaaacacaag cgtcaaataa agccgatggt gaaaaagtgg
    13081 ttgatccatt agaaacgatc gtcattaaag acagtgtttc ttataccaac ttgtttgtcg
    13141 gaaaagaata caccatttca ggagtattga tgaacaaggc aacgaatgcg ccaattttac
    13201 aagatggcaa agaaatcaca agtaccttgg cctttattgc agacaaagtc aatggcacaa
    13261 aagaccttga atttgtggtt aatgctagtg ttctgaaagg tcaaacgatc gttgtttttg
    13321 aaaaactttt ccgtgatggc attgaagttg cagcacatgc ggatatcaac gatgaagccc
    13381 aaagtgtgaa agtgaataat ccaaccgttc ataccacggc tgtctttgaa gacggattga
    13441 cagtcgcaga cccaacagga ttgtttacct taaacgatac cgttgaaatt accgagattc
    13501 ttccaggaaa agcatacttg gtcaaaggga tcttaatgga taaagcaacc ggtgaaccct
    13561 tgcttgtgca tggaaatacg gtagaaacga gtgttggctt tattgccaaa gcagaaaatg
    13621 aaacggtgat cgtaccattt acctttgatt tgacaggtat aaatggtcgt gaaatcgtcg
    13681 tgtttgaaag tctttttaga aatgatgaag aactagcaaa ccataaagac atcaacgaca
    13741 aagggcaaac ggtacgagta actaacccaa gtgtacgtac aacggctaca cataaaccca
    13801 ctggcttgaa agaggttaat ccattaacga aagtcaccat tacagacgct gtgaaatacc
    13861 acgatttggt cgttggaaaa acatacacag tcaacggtgt tctaatggac aaagtaacgg
    13921 aaaaaccagt gctagtagat ggaaaagaag tcacaagtac cttaacgttt gtggcagaaa
    13981 caacggatgg tacagtcaat ctagacttta ttctagacgc acgtgcgttg cgaggcaaag
    14041 aagtcgtggt ctttgaagat ctgtttagag aaggcgtatt attggcttct cacgcagata
    14101 ttaccgacaa tgaccaaacc atcaaaatca gtaatccaac cattacgacg aaagcgacga
    14161 ataaaaatgg cggaaaagag atcttggcct tgccaaatca aactgtgctt gaatgggtca
    14221 aagtagacgg tcttgcgatt ggtcaaatct atcgcttaat tgttcaaggc tacttaacac
    14281 cagacggcac accatttgaa ggcactcatg cggaaaaagt ctttgcggcc gataaagaaa
    14341 tgatggaatt cttatttgaa ttcttagtgg acggtcgcaa tcttgcagga aaaggcttga
    14401 gttttgcgga atggctccaa gcgaaaacgg aagatgagaa agaaacgttt gaagaagtgg
    14461 cgaaacacaa tgtagatcta acaaacaaag atcaaacggt tcaaattgtg gaagcgccaa
    14521 aaccaccgca accagtgaaa ccagaacaac gggttaaacc agctactgtt gtaccaatgt
    14581 tgccaaaaac aggtgaacaa acaagtagta tacttatggt tcttggatta gtagtattag
    14641 tcattgcttt aggtataaca ctttataaag tacgtaaagc caaagattag tcatgaggaa
    14701 caagcggtta ggatttttaa ccgcttgttt gattgtaatg tagaaatatt tagaatttat
    14761 tttggttttt aagaggagga aaaactttat ggcagaaaaa gcattgcgta tgaagaaaga
    14821 gtttttaggt caagtatttt tattgggttc acgggaacct aatccaccaa aggaaattaa
    14881 tggtgtgtct gggcgatatg ttcaattaca agcagagtgt tcgcgagaag aacagcctgc
    14941 tttttttgtt tatctcacag ctgaagagga taaaagatta cgattaccag aaaaagaggt
    15001 gcgtatttca tttgaagaag cttattttgt ttttcgtaaa gaacgagtga atagtcgtga
    15061 aacaaatgat atgggacaaa cagagcgtcg aacaactggc tatcaagatg ttggtgtctt
    15121 tttaactggt gtaaaaattg gggaggtttt gtaagatggg attaaaattt ccaaataatt
    15181 atgacattac agaagcatta gatgaaactt gtttaggagc cgtacgcttt ggcgcattag
    15241 aagagtctga tcgtgtattt gataatgaaa caggcgatta tacgggagaa attcgatcac
    15301 agcgagcaca attagttttt ggaaatccag atgaaccaaa gattgcacga cgagttaaat
    15361 ttccagcaga tgtagatttg actggtttta aacgacgtga tcctgtgaaa ttaattgatc
    15421 cagaatatca tgtggtatat attccagcac aagatggcaa tcgtgaatat tcagatttag
    15481 tgatttatgc gaaaggatta gagaaagtag aagctccaac aaataatcct aatatgaaac
    15541 aatcacaaga aaaaggttta gatcaaaaac aaccaagtgt tgattctaag gaaaaagaga
    15601 aaaaataagg tttttttgaa agaataggaa ggagtcttcc tattcttttt atctaagata
    15661 gaaggaaggg gagattttga attcagataa aaccaatcaa atactttgag agattgtgct
    15721 tgttatcaaa atatccaaca attgattgag tattttggag acaatccaac gatttttgta
    15781 caaacaaatg catatatttg tgcggttatt gggcagtgta tgtttttacg tgatcttgtt
    15841 cgtttttcat taccgtacca gtggaaacaa gatcatcagg aagtgatttg gattcgtata
    15901 gtacgaatat gtaatatgtg tgcacaccct tataaaaatc gattaaatct ttttcgatta
    15961 tgttttttta ctaaatgtga tattcctgta ttagttgctt tttgtcaaaa gtttttagca
    16021 caggaaaagt aaaaacagaa aggaggaatg tgacaattga aagctcattt tttcaagtat
    16081 cgtggtcggc gaattcgcta tttcaatcgt tatttatggt tttgctatta tggtttcttt
    16141 ttgtttccat ttttgattct gttaggttgg tttagttgga aagtatttta tccacgttta
    16201 agtacgtttt cgaattggca aatgtatctt attcctggaa taagtattgt gttgtatttg
    16261 ttgctggtta gtgggttaat tttagggttg atgcattggt cgcgactaaa agaaggatat
    16321 tttgcctctg tttattggcg tcagttactt gttcgaatgt taattgacaa tcggtttgtt
    16381 tatacaaaga aacaagttag tgagaaacaa acaagggaaa aaatgagatt accaaaagtt
    16441 tatttttatc aaaagaaaca agaattagtg gtttcttttc ctttagatgg tgggcgtttt
    16501 cacgaacgat ttttaaaaat gggtcatttg ttatctgaag tatttttgcg tgatttaatt
    16561 cgtgaagaac gagaaaaagc ccgaatcaat tatgtattcc tttcagattc tggtcggatt
    16621 cctattgatc aatgtatagc agaaaatgga cgtattcatt taatgaaaac tttggattgg
    16681 gtatatgatc aatcaccaaa tatgttgatt gctggtgcaa tcggtggtgg gaaaacctat
    16741 ttattgtatt ctttaattca agcttgttta agtgttggga cagtagatat ttgcgatgga
    16801 aaagcggcag atttagcagc attaggtgac gtcgatgtat tcaaaggaca tgtgttttac
    16861 aaaataaatg aagaaatgat tatttgttta cgaaatgcat taaaagaaat gaatcaacgg
    16921 tatgcctata tgaaaaccat gaaagatcct aaatatgaac cagggaaaaa ttatgcttat
    16981 tatgggttac caccgcattt tatctttttt gatgaatggg cagcttttta tggttcgttg
    17041 gatatggaag cgagaaatag agtcgataaa ttgattcagc cattagtatt aaagggtcgt
    17101 caagcaggga tgtacattat tttagcaatg caacgaccag atgcagaata ttttccaagt
    17161 ggtgttcggg ataatttaac attccgtatt tctgttggtc gattgtctcc cattggttat
    17221 ttaatgactt ttggagaaga ttttaaaaat aagccatttt acaacaaaga tcaacctggt
    17281 cgtggatatg cttttccagg atttgatgta cctgtagagt tttttgcgcc atttgtttac
    17341 aaagattatc actttatcaa agaatttgag aaatttactg aaatgattgt ctatgatttt
    17401 acagtaaaag aggatagtca agatcaaacg atgggagatg ataatcagtt gttacaagaa
    17461 caactagagc gccaaatgta tttagcaaaa gatccaaaag gaacaagttt ttctgatgcg
    17521 catattttgt aatcaactaa ggaggtgaag ttaaatggca gagagttgtt attatagagt
    17581 agctacagca atacgaatga ttaatccttc tctttcttcc agaacgtttt atgattggtt
    17641 gaatcgtatt gaacaagtaa ctgattaccg ctttttgcgc aaagaacgtg tttttacagg
    17701 aaaagttatt aatcaagtat tgctaacgaa aaaagatata gaacgattga ctcgattgta
    17761 tcattatcga gtagatttag aagaggattt aactttgtca atttatcgcg ttttttctcc
    17821 tgaaaaatat agtgaaataa caaaattaga tcatttaatt ttgtagttat tatctatcgg
    17881 gagggctcta gacgtagtct ggtagccgca ctctgcctgg cggtgcgcat taaggctgcg
    17941 aagcatgggc ctataatgcg cactcgctag cagcgtgtgc agctcacaaa gaggtcgtgc
    18001 gcttgcgcac ggcctctttg tgtaaagccc cccgatatat aatatggggg gcagaaataa
    18061 cataacacat agaaaaaagt tttataaacc cttttgtatc aagtgattat ctaaggtcag
    18121 cagttgtact acttttccca aaaaacgagg gggactagtt gatatatcgg tgtttctaaa
    18181 gggttaaaaa aaatcgattt tgttatttta taaaaggagc gagaaaatga caccgattga
    18241 gctaaaaagc tacagaaaga agttaagtgc aattcataat ataagtggag ataaaaagcc
    18301 gctatcacaa gcaagatttg cgcaattgat gggctgctca aaagattaca ttagaaagat
    18361 ggaacaaggg aaaagaaaaa tcccagagga ttttgaacaa cgaatcaagg aacgctttcg
    18421 attgtatatg aaaagcggaa tagatcttcg agcaatgatt gattatttgc gtctttcctt
    18481 ttttgggaca gactacaaaa cagtgattca acgtgtatta cgaatggatc tgacagaatt
    18541 tgaaacacgt ccaacgggct tgtatctttt tgactgtctt tctgttcgtg ggaatatctg
    18601 ggtgttctgg cattcaaaac gaactgtaac agaaaatgta cttgtgcaat tttcaggaca
    18661 aggttgccgg gaatatgagg aagtcatgcg tgaacaacaa acagattggc gaacatggct
    18721 atattccatt tggaaaaatc aaggaaagtt ggaagctggt tatacacgtg tgcaatgtag
    18781 ccgattcgat attgcgttag atgaactttg gggattggaa acggaagaac atttcgatct
    18841 ctttgaatta ctagaaaaac aaaaagcagg tttattagaa atggacttta agagattcca
    18901 gaatattggc ggttgttttt tagatgatgg ctattttcga agtgaaggat tatctttgta
    18961 ttttggatcg cgaaagagtc ctctcttttt taatttttat gagaaacgtt ttgaaattgc
    19021 gaaccgagaa aaaatttcag aattagaagc attaacgaaa tatggtattt ataatcgata
    19081 tgagttacgg ttagccaatg agaaagcaac acaagcagta gaagcattta ttttaggtga
    19141 ttcaccgaaa cgtttaggag agattggcgt tggtttaatt aatgcaaaac ttattgtttg
    19201 ggatagactg gaaagtgatg cacggattgt tgataaacga tgggcaaaat tatttcgttc
    19261 gatggaagaa ttggatttta cagtaaagcc agaaaaattc tcattagatc gttcagaacg
    19321 ctggtttttg aaacaagtag ctccgacatt aaagttgtta caagaagcag atcgtatttt
    19381 aggaagagac tttgtacaag aagggattga taatgccgaa ctaaatgacg agcaacaaaa
    19441 ctatctagaa tggatgggaa cgtcaggagg gaaaattgca tgaatgcata tacgacaaca
    19501 gaatggctgc gcctgttgga tttaaaggct gcaatcgaag cattaaatga aaagatggtt
    19561 gatttgtctt attttcgttt tcgagttcct tatattgaac aagcggtaaa agctggacgt
    19621 tatcaggaaa aagagaactg gcaagaaatt gcacgcttat tagaagttcg taagggatac
    19681 gaacaagaac tcgaagagtt ggaatttagc cgaaggaaag gaaggctaga attcattcgc
    19741 ttttatcgat tttcattgcc aatacctgcg attttagcgg ttaaaaaagg ctgcgataaa
    19801 atgaagatat atgaaaactg tgtggcagca cttagcagcg aaaaacctct tatggaagaa
    19861 atttcattgg tgactgtcac ttgggaaatg agtcgccagc caacagaaga acagacatat
    19921 ctttctttag aagagattga gatagaatta gaggaaattg gtcgctatgc cacttgttct
    19981 acctattgtg gatcggtcat tagtattgct ggtgtgattg tctaaatgaa aaaaattatg
    20041 atttatgttg gtgcgtattg gtcacgtgat ccaacagtat tagaaaatcc agaggcggta
    20101 cactattgct tgcgccaatt attctatctg tataaagaac gattagaatc gttgattcgt
    20161 cagttaccat atactgacag acgtttagat gaacttcttc taaggtatcc agcaatgtat
    20221 aaacgacgaa aaaatagatt gttaccagaa gagtatccca ttgaaaaaca tgaacttgaa
    20281 gggcgatttg tggcttattt ctatgatgat gttcgtatgc gcttagtaga acaacgaatg
    20341 gaaatagaaa atgatcgcta ctattttatc aattattgta agaagcgtaa gtatcaagta
    20401 actgatgatt tttatgattg tattttacaa gatggggaag tgattctatc caaaattgct
    20461 ccaagttttc gtgaacttgt gccgattgat tttttgaaaa aatgtcattt gcggattctt
    20521 ccgtaaaaaa gagctataaa aggaaagaaa agtagaagcc aaatcggttt ctgctttttt
    20581 atttttttta ggaaggagaa gatggtatgt cactggaatt tttaggagag ccaattgaaa
    20641 aggaagagtt cgtttcgcat tatatggatt tgtttattca ggtgataaaa aactttgtgc
    20701 gattgatgaa ttttatccac gcgaacaaga ctatctagcc gctgaacgaa taaaaatcag
    20761 tttattatgg gaaaaattca cagatgaaaa taaccgaccg ccagattatc gctatttatc
    20821 agattgttta acgaataatg tgctttctaa agaataccta tttcacaaac gtgagaaaaa
    20881 tcaaatgtcg gttgaacatt tttgtgattg ttatattgca ttattgcgta gtcaacagtt
    20941 attaacagta gaacgatatg gtgcagagtt tggttatgtc atgcaagctg agcgtcatag
    21001 agctacaaga tgctatgaaa aagaagatca agtgattgat ggctatgaag aattatgtat
    21061 tcgaaacaat cgctatttgc aacaacgttt atatgaacaa ttgatgatgg atttttcaaa
    21121 tctctataga gggtatgaat gttcgatgat tcaagaaagg tagaagaaaa gggggaattt
    21181 ttatgtggcg attattattt ttggtggtat gtggtatggc attgttctat gtgtatcgtg
    21241 tctatctcta tccacgaatg gaacgatgga caagttggta tagagatcct ttaaaacaac
    21301 gaaaaggatt gaagcggtat cgttatgaat tacttgttga attttcggat gggcgacaag
    21361 cacagtatta tttttttgca aactatccaa aatacaacac cagtgaacta ttgattcaat
    21421 tattcaaaaa taatccatat gttgggttag aagaaaacgg acgggagttc ttttatcgtt
    21481 cagataatat tagtgcggcg tctatgaaca aaacacgaat tgcataaaaa attggaggaa
    21541 taatcatgaa aaaaacaaca ttagcagtcg taacaacact aggaatttca ttattttcgt
    21601 ttcaattagg gagttcattg gtttatgcag atgaaactgt agaaacacct aagtcagaaa
    21661 ttgtagaaac attgccaggg gaaggattac cagatgttga gacagagcta aatggaggaa
    21721 caacggaaaa accagcagaa cctgaacaat tgcagtcaga gaaacctgaa aaagagccag
    21781 tgacaccaga accagagttt cctgaatcaa ctttaccgac tgattcgatc gaagaagaag
    21841 gtgtgcatag ttcaacaaca acggattcaa gtatagtcac aactccggaa caatcaggaa
    21901 caaccagttc ttctgaaaag aatactgatt cgtcaagtac ggaacagcca acgatgtcgt
    21961 ctaccagtga agaacaaaaa gaagaaccga aacaacctgt tgaaaaatca gaagcaattc
    22021 caaaagaaaa taaacctatt gttccagcaa aagaagtcac agtatcggta accccaagcg
    22081 gtgaaattac gacgaatact tctcaaggaa tgagtgtacc gattgtcact agtaatgtag
    22141 aggaattgac ccatattcca acaccaacca ccccattaaa agtagcaggt gatcaaacaa
    22201 ttgttggtgt aaaagatggt attccattaa tccaagatag tgaagggaat ttggtaaagg
    22261 atttgtcgat tcctgtaaag aaacttccaa gtggcaatat tgaaatcaaa acagcagatg
    22321 gaaaaacaaa agtcttacca aagactggtg aagaaataca tgttgcactt tctgttctag
    22381 gaggagtgtt gacaagtatt gctggatttg ttggatataa aagaaagaga aaaggaatag
    22441 aaaatgaggg taattgaatg gactattttt cagaaaaaca agcttatcgt tgtcaatcgt
    22501 acgtactagt aaaaacatac tagaaggagt agcttatgaa aaaacgacaa ctacggcgga
    22561 aagaaaaagt ttatacagag aaagtaccga agcaaaagaa aatccattca aaaaaagcag
    22621 tgaaatggat ttggtgtttg gttttatttc tttgtttttc agggggcttg gcattttttc
    22681 aatccatgaa aacgaaggtt caagtgtcgt ttttgaaaga tgaaatccaa caattacaaa
    22741 agaaagaagt acaaagtcgg tcagaattaa cgtacacacc tgaaattgaa tcctttatga
    22801 atcgttttgt tgctttgtat atgaatacat tcgataatca tgaacaagaa caacaacgtc
    22861 aagaaacatt aaaaaaagag ttttatgcac aaaactttat tcaagaacca ggaagtgttt
    22921 caggtgttcg aaaattaact gctgcttcgt ttattggatt aaaagaaatt gaaaccattc
    22981 caactgctag ttataaagta acttatgata ttcgctcgat gaatccagag agtaagaaag
    23041 aaacaagtgt gacaatcaca caagtactca atcttccctt ttctatttca aacaagggct
    23101 gtcgtattat tgcttatcct tattttacgg cactaccaaa gaatacagaa aaacaacgaa
    23161 tgattactta tgatgacaca ttatacgaaa cggttgatgc gtcggaagcg aaagatgttg
    23221 aaggatttgt ggaagatttc ttagataagt atgcgaaaaa caatgcaaaa gatatgtcat
    23281 acatgatgaa agaacctgaa ggactcaatc aaacagctaa agttgatcaa gtagagatga
    23341 aagtattgcg ttataaagaa acgaaaaaac cttttattgt aaaagcaacg gtcgtctttc
    23401 gagatggtga tacgtcactt ttacaaaaag aacaaatgac gattgtcatt acgaaaaaaa
    23461 caaatcagta ttatgttgaa aaattaacac ataattggtt ggataggaaa ggagaagaac
    23521 ggaaatgaca ctgaagggaa ttgtagattt tatattaacg caaggtggct attttttaat
    23581 aatggtggtt gtttatctgg gattgcgaaa ttttatgaaa tcaaaagtag gaaatgcatt
    23641 aatatctatc gcagcaggtg ccgcagttta tttcttctta aatgatccaa cacgtgtatt
    23701 tgatgcgttt ggtaaaatca ttgaaaaatt atttatggat taggaggata gtgaagtgga
    23761 ttgggaacct tatgattatt cgattgttta tgcggaacct tatcgcgtac aacagattac
    23821 aagcaattgg cgtcttccat ttccagttgc attagtcaat gcagcaactt ttttgttggt
    23881 cgtaatcttt gaatatttat ttttacgttc atttattggt tgggtcattt ctttttttcc
    23941 accagcagga gttgtttttt atataggact cccctattta tgtgtgttag cactggcaaa
    24001 actaaaacct gatggaaaaa aagtgcattt ttatttatgg gattggtttt gttatatgat
    24061 tcgtatgatg aatggtgatt gtgtgtatta taactgggac aaggtatcaa acaatgcatt
    24121 ggaagaatta ctttttgtga ataagtatgt ttatttagag gattagaaaa agggacaatc
    24181 aactggttgt tcctttttta ttgggaggtg aaattcattg gtgcttgtaa aagaaccagt
    24241 aaaagcattc aagaaaaatt gtttgttaaa tggagaggat tgtatctggg cctactatca
    24301 tgtttttcca cgaacaattt caaaaaacac accaaaaaag acacaggaaa taaaagaaat
    24361 gatggctcgt ttactgttgc ttttagcaga ttatgaagat gtggagttgg tgatgtatcc
    24421 acgaacaatg aatttacgga cgaagtttga cacattagga aagggatttt ctcccgatag
    24481 tcaagcaatt ggtgcgtatt atgcaaaaga aacagtgaca ttgttggaac gagaaggtca
    24541 agtctatcgt ccttcattta ttttaggaat caaagtcaaa gaaaaagcaa cggtggagag
    24601 ttttcatgat gtaataaatc taatctggtt agatttagca ggaagtttag cacgcttatt
    24661 tggattagaa gtagatgatt tggaaacttt gttttctgaa agtgatcctt atgtaaaaga
    24721 agtgacacaa ttactttata gcttttctat tcgtccctta gtaaaaaaag agctgttgta
    24781 tctcaatcgt ctgcaatata ttcgaggaat ggctcacaaa gtagaagaac aaaataattg
    24841 ggaagtactg aaagacaatc aaggaacgta tgtacgtata ccgaaggaac ataaaggcct
    24901 tcttcaatta gaagatgagt tagggagtag ttatttagca ttgttgccta ttggggaaac
    24961 acctgaaaat atgagaaatt gtgatctatt tcaatttgct caggatcttt ctttcccagc
    25021 agaattgcga atgaaaattc actatccacg cttgaaagga cagggaggta ttggtttaaa
    25081 actgaattgg ctatcggaac ggttcaaaac agagcataag gaattggttc aaaatgatga
    25141 agaacctagt gaacgcttat taactgttcg aggattagta aaacatttaa agcaacgagt
    25201 agataatggg caacctgtcg tcgattggat cgcatgtttt attgtatacg gtcaaacggt
    25261 tgaagaagtt cgtcaaaata gtgcaacggt tattcaacaa cttcatacga ttatccatat
    25321 ccaccgtgca aaaaatgatc aacgagaatt gttctataaa tgtttattag ggcaaccatt
    25381 gggacggcag ttacattgga agcaccgaac aactgtagaa gcagttggcg aatggttgtt
    25441 ttcagcagta tcagaattag gtatggaatc cggctggtat attggtcggc aagatacatt
    25501 aggggatggt ggttctcgag gagaaaaaac accattagaa gaattaattt atgcgtcaaa
    25561 tcgttttgtc tttttgaatc ttctagccat tgcggaagga atcaaaggtg cgttgtttga
    25621 tgcgcctcat gtagcagtaa ctggaaaaac cggtaaaggg aagacctttt tagttggctt
    25681 gctctttttg tatagttcgt ttatgaatgt acaaagttta tttgtggatc ccaaaggaga
    25741 aaagcgcaaa tggtttacga aactcattca agacccgtat tatcagaaac attacccttt
    25801 gtttgtagaa catttgcaaa aaatgagtta tgtcacgtta gatgctacaa atccccgtaa
    25861 ttatggtgta ttagatccaa ttgtttgttt atcccgagta gatgcgaaac aagtagccca
    25921 ggatatgatt gatgagttaa gtccgttagg taccaatcac cttttaaaag gggcggtatt
    25981 aaagggaatc caagaagttt tagttcaaaa agaagccgga caacaagttg gcttgatgca
    26041 tgtggtcgac tatttagaac aaatggaaca aaaagaggtt cgagaatatg gtgagttttt
    26101 acgcttgacg gtagaagatt cgattttacg tttaggattt tcttatggag aaaacccggg
    26161 attagacttt aatgccaaaa caacgatttt agagatccaa gatttgaaat taccaaatga
    26221 caatgtccga ccagaactat atacagatgc ggatcgaaag tctttgtgtt tgatgattag
    26281 tttaggtcgt ttttgtgaaa tgtttggaaa acgagatagt agtaagaaaa cagcgattta
    26341 ttttacagaa gcatgggtat ttaataattc gaacgcagga cgtagtatta ttgcagcaat
    26401 ggcacgtgtg gggcgttccc agatgaatca attagtgtta gatacacaat ttattggtga
    26461 cttaggttca gataaagaaa aagggaattt tggtgtcgtc tttgcttttg atgaagacag
    26521 tgagcgagaa aagattttga atcatttacg tttggaagta aatgaagcga acttagctga
    26581 aatgagccac atgattaaag gacagtgttg gatgttagat ccctatggac gtgtaggaaa
    26641 actcaatgta cacagtttgt ttgaagaaat gaccgctgca ttacaaacaa cggaaaaaac
    26701 aagtcactct caagtagaag aacaggatac gttaatcatg caatagaaat gaggtgaaac
    26761 aatggcttat ataaagcata aggaaaagaa atggtgcgtt ttgttttttt taacgattgg
    26821 attcggactg ctttttgttg gtggattggt gtatgcggca gaaaagcagg tggatcaatc
    26881 gatctatgag attgggaaat ttcatattga taccttttct tcttatttat ctgatagtga
    26941 attttcatta ggcaaaatgt tatttggggc aaatggcaca gattttctaa aaatgatgac
    27001 gaatatgttc tttagtttaa gtaaattgat ttggcaaggc cttgattttg tcattgagaa
    27061 gctctatgaa ggagctgcaa tgaacgagtt aattcaccag tttttcactt tttcggatac
    27121 tatttattca aaaatatacg atcctattgg tgcggggatt gtcgtcttgt atgtgttata
    27181 tctatttact cttcgtatat ttagagggaa acattacgca aaaatggtgt ttattcgatt
    27241 tctcttcgtt gtcatttttt caatggtttg gtttggctat ggtagtaacg tacctagtaa
    27301 aggagaagct tttaccaaag ggttaaatga tttgtccgtg gaggtagaag gctttatttt
    27361 ttctgctaca aatagtctag atgaattggc gacaaaagat gtcaatggtg ttttagtgcc
    27421 aacgacacaa aaggaagcca ttcaaaaagt gcgccaactt tattataaag cggctgttgt
    27481 tgatccgtat atattgttaa attatgggac aacaaattta gaagaattgt atcaagcaga
    27541 gattgaacca caagaatttc tagccaagga agcgtcaagt gaagaattat ctgatattga
    27601 gacaaaagtg accactgctg caggagaaga tagagggaaa aagaaggctt atcgtgtcta
    27661 tattcaacca actaaagctg tttataaagc agtcgttggt tctatggtgc cattgcttaa
    27721 tctaagtatt ggattaccac ttgtagtgat tggcatgatt cgttttatct ttcaaatgtt
    27781 ggtgttgtgt ttattacttg tgattccttt ttcattactc gcttcttttg ttccagtatt
    27841 tgattatatg ctcatgaata ccttaaagtc ctttttgggc ttgatgtttc aaaaatcttt
    27901 ttacagtgtg attattttgg tggcattttt gatctataac atagtagacg atttaattcc
    27961 aatgaatact gtttttggtt ttttaggaaa tatgtttgtc aaagcactcc tatcaatttt
    28021 actcattgta aaacggaaag atttattaaa gaaactaggg ttaggagctg cagatcaaac
    28081 tataaacaac acgaaaaaca tagcaagtca ttctttgtct caaatgaaac aactgcctgg
    28141 tcaactatca actgctggac aaaatttggc aatcaaaggt gcaggattag cagggaatgt
    28201 gtatcgagca acgaatccac tcgcttctgc agcgatacat ggatttcaaa agtttcaaca
    28261 aaaacgtaca ccacaagaat cggtaaaagg gaggcaagtg gttaatccct ttgaactagg
    28321 tgggcgttca aatcaagcct tgccttatag tggtcgtcat gttcgttatc atggggtaga
    28381 gcctgaaatg tcttctacca aatcgaaaac agagtggaat tctggtcttt caaatggttt
    28441 gattccaaat tcgtccatga attcagtaag accaaaacaa caggtacaat cattggcaca
    28501 attgagtaag caacaaggga ataatcaaag aaaaccattg ttaaataaag gtatcgcttt
    28561 tgttggtcaa tcgattccgt tgccttctgg aaaagtaccc gtgccacaat taacagctgc
    28621 tggttctctt tcacaacaga ttcgccaagt gaaaaatcag gcgaaaaatg ggagttatcc
    28681 agcaatgcag cagttattga accaatactc tgttcctaat tcgtcggtag ccactctatc
    28741 aggaaatcgt tctgtgcaac aaccggggca acgttcgtct gaaagtaaac cgtcgatttc
    28801 cgcaccaaaa tcgttacaac ctgcattttc ttctttagat aagccaacag agcaatcgga
    28861 aaaaatagaa aaggatccta aaacactgcc gattttggac gcaaaaccag gaactgattt
    28921 tactttacca aaaggacgaa cagtccaaaa accattatcg aagaaaggat gaggaagaaa
    28981 tgaagaaagg gtgtctaggt tgtcttcttt tctttattct tccctttttt ctattgccat
    29041 tctttttttc aggagaactt tctgagcatg gaggagaagc tacaggtcta gctgtcgctt
    29101 ttaatgggga atataccgaa ggattaccga ttttttcaga gattaaagga cgtggtccaa
    29161 tcacagatga acatgcgcaa gcagcagttg gtgcagctgt aaagtatcag ttattaccga
    29221 gtgtgattct ttctcaatta ggctatgaat cagcttatgg acagtcacaa tctggaagaa
    29281 acgatacgaa cttttttggg attacctggt ttcctggttg tccctaccca caaggttctg
    29341 cacgtggaat tggtggttct gaaggtggtt tctatatgaa gtttccgaac ttaaaagctt
    29401 gttatgacta ttatggcttt atggtggcga gccaaagcaa ctttaatcaa tgtgtgggca
    29461 ataaagatcc tggaagttgt ttattgattc taggtcgtgg aggctatgcg gcagcaggta
    29521 ttaccgaagg gagtccgtat tatcgaggag caatgagtat cataacgtcc tataacttaa
    29581 cggaatatga tacttttgcg atcaagcatt ggaatagcct accaacaaaa aaaacttcgc
    29641 ttattgcagg aaaaagtaaa ggcaatgcga aggttttgga tcggatcatt ggacgatccg
    29701 taaataatgg ggaatgttat ggtggtacgg catggtatgt atctcaatta ggtggcccac
    29761 agttaatggg ttctggacac atgtttgcga tgttaatagg ggaagattat aactggacaa
    29821 cctatggttg gcaagtaatc atgaacccaa aaccaagtga tttacaagca ggagatgtgg
    29881 tgaattggca agcaggtggt gcattgtctc ctggaatcta tggtcacacc ggaattataa
    29941 cagatgtttc atatggcgga caacatttct ctagtgtgga acagaatgcc gagcaaggac
    30001 ggattatggc tcggtatcaa cgcacgtaca atcaaacaaa aattcgtagt ctggttcgta
    30061 aggtgaaata agaaaggcag tgactagaaa cgtgaaaaaa gggataattt tagtatttag
    30121 ttggttatgt ttgataggtc ttactggttg tcattggttt tcttctgaaa aacaaaaaac
    30181 acaattgaga acgacagaag aaacaatcga agcagtaaaa gaaacaagtg gaactcttca
    30241 aagtactgaa agtaacagtc aaattggttc attagaagac gagacagcgc aacaaatgca
    30301 aatagatgaa atgacaaaaa atttcttaaa agattttgct agaaaatgga gtaatttcac
    30361 agatgtttat aaacgcaatc aagctgtgag ggactatatg acagatcgtt gtattaaaga
    30421 caatagtatt gatgtagacc cgcatgtaca aatgaaaaca actgggaaac tttaccaggt
    30481 gacacaagat atagagaatc cggatcaata tttgttattt ggagaagaaa caatgaataa
    30541 ggttactcaa tttgtattac ttcaattgaa agtagatcaa gaagctcaaa agattgatgc
    30601 gatcaagatt tattatgtac ggagtgcgta ttaaaataaa aaatgaacac tcatatttat
    30661 tatacaccga agaaatagaa aaaaacagac tgttttttta agaaaaacta tgtcattctt
    30721 tacttgaaaa aagttgttat aggacagaaa ggataaaaca aaaacggaaa aaatatgtct
    30781 tatgaataac tataagaact attctatttt ttaggagatg gatacgagat gaaaaaacta
    30841 attggctata gtgtgatgtg tattggattt gttggtgtgg gattgtttgt atcgactgaa
    30901 tctgcttctg cggctgaatg gtataatcct atctggtata atccgaacat tgaatgtcaa
    30961 aaaggctatc cgtatggacc gacaaatctg aaaaatggaa aatgtagagt aaattggaat
    31021 ggcgttgtag atagtgtaat tactggttca atggactctg ctctacatgg ctttacgggt
    31081 cactagaagg aggaagaaaa tgtctgaaga gaaaaaagaa atcgttgaat cattagtagc
    31141 tttaaaagat tctctttcta agattgaata tgttgatagg caagaaattc aaaaactaat
    31201 tgatgatact attattgaaa tccaagatgc gcgttgtgaa ggtataaaaa tttctgtagc
    31261 tttatctaaa gtaatagaaa aaatgaaccg ttcattagcg tttaatggat tgaagctaga
    31321 tagacaaact agtctgatat gggatcattt aaaagacttg tatgacaagt ctaaaagaag
    31381 tgaacggact gctgtatcaa tcttaaaagg cctttgggga atgaattcat aaacaagata
    31441 tttaaaaagt aacttatttt ttatggagga gaaattatga aaagaataat taaaatattt
    31501 ggtgttttag gtgttttctt attaggttta agtacttttg ttagtactgg aacaagtgtg
    31561 tatgccacag aaaaagtgaa tacagttgat ttagaaataa agaataaatt agtagatgaa
    31621 ttaacttttt attttgatac agttggtttt ttagacgaac atggtgtata ccatataaag
    31681 gattttggtg ctattcgtga acgaatcagt caagataacg aacaaggaag agcagcaaga
    31741 gtcttttatg aacatattat tgttcctaga atggagaaag ttgattggaa acgatatgct
    31801 ggatgtgtag ccattaattc tcttcctgga ggaggagtgg cttgggctat aggtacgcag
    31861 ggagatttta ttgaagcatt aaaagctttt aattttgaaa aagctgcgga tttattatta
    31921 aaaattgcag aaaaacaatt aggaaaagct gcagctaaag agttagctaa ggtaaatatt
    31981 gtagcaggaa ttgcagcagc tgctgctagc tgtgcattag agtaaaagtt tatgaaaagt
    32041 ttagaacaag agcatatggc ttttaaacaa gcaatgttta aagaaaatat ctatttaaac
    32101 cataactata ttcgtgtttc taaggcatgt tcaccagtat tgaatatgct tggaggtgga
    32161 aatggattat atcatttact gtttgtcgat gtttgttggt taattttttt accagatgaa
    32221 ctagttatag tcaatgaaaa aataacaagt aaaaatgaag tgtttaatta ttctttaaca
    32281 agaattaatt acaaagagat taccaagttt tcactagaaa aagttccttt ttggggagaa
    32341 ttttgtttaa aaattaaatg taactggaaa agaatgtatt tctatattga tggtgatgat
    32401 gctcttacat tcggtaaaac tactttttct agttttaatt ttcagtttct attaaaaaat
    32461 aatttttatg gtttattgaa ataaggttgc gaaggaagat gtagagttat ttgcactgtt
    32521 gtgttggtat atctcttaat gctgtgtctt tttggggaga ttaattagtg gagtgattat
    32581 atgagtatag atgattgttt agaagaacaa ataagattat ttttaaagtt tttaaaagtt
    32641 aaagttcaaa ataactactt cacatgttat agttatgcac accctgtttt aaatagtctt
    32701 ccttttaatt ctttagtgca aggattgatt acaaatattg ttttattagt ctgtacacca
    32761 aatgaaatat ttgtaaaaaa gataggcagt ggtatttctt cagtaagatt aaaagagaaa
    32821 gaatatactc aaaagattat tcatattcct aagcctgaaa ttaagcgttt tgagataaaa
    32881 gactattctg ctttagggct gttctcaatt ggttactggt gataaaaaat aagaatacta
    32941 aaactatacg tagaatataa gtaatcggca tcaatcctaa ttatgtgatt gaatgccgat
    33001 ttttgttacg cgctattttt aattatctgt aagaagaata attttatttt actcatattc
    33061 gttttcactt tgaagaagta aattgcaatt tcttataagt taagcagcta tgcaaaaggc
    33121 tatttattct attgtgaaat ataagatgta gttgaaacag gatatataga atcaaaatac
    33181 gaatcttatt ttgatttttc tagttttttg aaaaaagcac cctttgatcg ttgtattagt
    33241 ggaaaagaga aaaaggagca tacttatcat gtcacaaaac tacaactaaa tatctaaaca
    33301 acaccaattt gatgcctatt gaaaaaaagt attgcgaaat gaagccaagt ctattagaaa
    33361 atgaaataat agaataaaag aaaatgggaa accgctgaat gatgtaaatg aaggtaagta
    33421 tccacgaact aattttgatg aacaagatat ctatttttta tttgtattgg agatactagt
    33481 ttaatatcaa aagttatcag cagtcataat tagttatcca atgtaaaaag tacaaacaat
    33541 agttgttatg cagaatagtt tatgaaatat attacataat aagaaaacaa gcgatatttt
    33601 tctgatcaga acaaccattt aatttaaaaa gagttaaaag ttatatattt ttgaaatatt
    33661 ttggatgagt agctaaaaca atattttgct cattaagcat taaatgaaga tgcaacgatg
    33721 ttagaaagtt agcttcttct ggatcttgtt ttgttaatgc ctcaaatagt tgataatgtt
    33781 gttccataat tttttcccac tttaatcctt ccgtttttac tcgtagccat cgaaatcgtt
    33841 ctaagtgtgt attgttattt tctaaccatt gccatacttc ttctcgctct gcaatttcaa
    33901 aacaagcttt atggaataaa ttatcattat gaaaaaaact acgagcattt ttttgactaa
    33961 cagctttttc ttgttctgaa attatagttt ctaaaatccg ttttttttgg ttattcattt
    34021 tggcacaaca ctctaacata atttgtcgtt ccaaatgttc tctgacaaat cgagcatttt
    34081 tggccgaatc taaatcaatt aatgagatat aatttccgct ttgaggaatt gtatcaacaa
    34141 gaccttgttg tcggagttga atcagtgctt cacgtattgg tgtacgacca atagaaagtg
    34201 tttcttctaa ttttttttct gatacttttt ttccaggttc taagtcagca tagataattt
    34261 gtcggcgaat agattggtaa gcttgatttt ggagactagt tttaattgtc atacacaatg
    34321 ctccttaact tttattttaa ctgatatttt aattctgttc atgccatata atatcaaatt
    34381 ttgaagtaat acactagtta taaaaataaa agaatacatg aaaaaaagaa aaaataagtt
    34441 tcgataaaat cagtccaaaa agaaggagta ttttattttt ctgaataaaa aatacaatca
    34501 ttctgatatg gtagaaaaaa gcaagtttat tcccttttta tattgacaac ggttacaatg
    34561 ggttttaaaa tataaaatgt atcaaaacta acatatcagt ttgaggaggt taattatggt
    34621 ttcgattaaa aattggcaaa attctatcga agaattcaat gaaatgcaga ttctactgcc
    34681 gtgttttgat gtcaaagaag ttaagcaaga aggccagcaa catccagtat ggcttcattt
    34741 tggtggagga aatctttatc gaggatttca tgcagaaatt gctcaatgct tagcagatca
    34801 aaatgaatta gaaacaggag tagttgtgtg tgaaacattt gatgaagaag tagtggaaaa
    34861 agcttatcat gactataaca atgacatttt ggaaattgtt atgagtggag atggtgaact
    34921 caaaaaaaga cttttggcag taacggctga tagtttatat tgcaatccga agaatatagc
    34981 ggactatcaa agagtggtta aatattttga agatcctaaa ttacagttta caacgtttac
    35041 tattactgaa aaaggttaca gtttaaaaga ttctaaaggg caattctttt cggtagtcaa
    35101 agctgatatc aaatctggac ctagcaatcc tcaacatacg atgagtattg taacatcctt
    35161 gttatatagc agatttaatg ctggagagtt gccgattgcg atggtttcta ctgataattt
    35221 ctcacagaat ggtcaacgtt ttcaagatag tatttttacg attgcagaag cttggcaaaa
    35281 agaaggatat gtttctgaaa aatttattgc ttatttaaag gataaagaaa aagtgacttt
    35341 tccttggagt atgattgatc gaattacgcc aaatccttca gagaatgtaa aggaagatct
    35401 attgatgatg gggctcgagg atttagatat cattcacaca aaaaaacata ccaatattgc
    35461 tgctttcact aatactgagg tagttcatta tttagttatt gaagatagtt ttccaaatgg
    35521 ccggcctaaa ttggaaaagg ctggtgtgat tatgacagat agagagacgg ttgataaagc
    35581 agacatcatg aaggttacga caagcttgaa tccacttcat actgctttgg cagtaacagg
    35641 ttgtctgcta ggatatcaat caatttcagc agagatgcag gatgatgatt tggttggttt
    35701 aatcaaagga ataggttatt tagaaggttt accagtagtt gaaaatccag gcattattga
    35761 tccaaaacaa tttattgatg aagtaattaa taaacgtttg cctaatccct atattccaga
    35821 tacaccacaa cgaattgcaa cagatacttc tcaaaaagta gcgattcgct atggtgaaac
    35881 aattaaaaag tatgtcgaaa aagacggaaa tgcagaaaat ctaacattta ttcctatcgc
    35941 tattgctgct tggttacgtt atttactggc agtcgatgat caaggggaaa attttgtgcc
    36001 aagtcctgat ccgttattgg tagagttgca ggataaatta tcagtattga aattaggtga
    36061 acaagatcct tcaaagattc attgtgcggt acaggaaatt ttagaaaata aagaaatctt
    36121 tggtttagat ttatatcaag ttggggtagg aaagaaaact gaaaaaatct tttgcgaaat
    36181 gctagtaggt cctcaagcag ttcgtaaaac aatcaataaa tatataacag aagttggagg
    36241 aaagtaacta tggaaatgac ttttagatgg tatggggcaa atgaagaaaa aatctcacta
    36301 caagaaattc gtcagatccc agcaatgaaa ggaattgttg gaacattaat ggatatacca
    36361 gccggagagg tttggccgag agaacgaatt aaatctttaa aagatgaagt cgaagccaat
    36421 ggacttacct tgaaagttat tgagagtgtt aatgttcatg atgatatcaa aattggttta
    36481 ccaacacgag atcaatacat tgagaattat aaacagacga ttcgcaattt ggcagaattt
    36541 gggattgagg ttatctgtta taattttatg ccagtatttg attgggtaaa atcggatttg
    36601 gattatcgtt tagatgatgg gtcttctacg ttagcctata tttcaaaaaa tatccctact
    36661 gatccggagg aaattattag agaagtagaa aattctacta ataattttga attgccgggt
    36721 tgggaacctc atcggttatc acaaattaag caattattag aagcctataa agaagttgat
    36781 gaagagaaat tacgagagaa ctttgcatat tttctcaaag caattatccc gacatgtgaa
    36841 gaagttggga ttaaaatggc agttcaccca gatgatccac catattccat tttcggatta
    36901 cctcgtattg ttaaaaatcg tgacgattta gattggttgt gtaatgtagt ggattctcct
    36961 tctaatggta ttacattatg tactggaagt attgcagaac gaccagataa tgatgtctat
    37021 gaaattctat ctgaattttg tcaacgggat cggattcctt ttgcacatgt tcgtaatatc
    37081 aaatatttaa gtgacaatgg actcgatttt tacgaagcgc cgcataaatc agaatatggt
    37141 tctctagata tgtataagat attaaaaaca atgtacgata atggatttaa tggctatatt
    37201 cgtccagatc atggtcggat gatttgggga gaaaccggta gacctggtta tggattatat
    37261 gaccgagctc taggtgcagt ttatttaaat ggtatttggg aagcgcttga aaagggggcg
    37321 agtaaataat tatataaggg agtttattta gaggaaaaat tttctcttct aaaaactaac
    37381 ggaaggaaag gagtgtttaa atatgttttt agataagaac tttttattag agaatgaaat
    37441 agctaaaaga ctttttcata aatttgcaaa acaccaacca attatcgact atcattgtca
    37501 tttagaccca gcatatttgc tagaaaataa gcaatacaag aatttgactc aggtatggtt
    37561 aaatgataaa ggagctggag atcattataa atggcgattg atgcgagcta atggtataga
    37621 agaagagttg atcagtggtc caggtgatga ttatgaaaaa tttttagctt ttgtaaaaac
    37681 agtggagaag gcgccaggta atcctatttt tgaatggtct catttggaat tacaacgtta
    37741 ctttgatatt gatttgacaa tttctgagaa gaatgcacca gaaatttggc gacgtgctaa
    37801 cgaaaaaatt caaacggatt cctatcgccc gaaagaattg ttggagatga tggatgtgaa
    37861 agccatctgt acaacggatg atcctacaga tgacttgtgg tatcatactg agctttctaa
    37921 acaggataat tctttcaagg tattacctac tttccgtcca gataaggtct ggagtatcaa
    37981 atcatcaggc ttttcgaact atattttaaa acttagtcaa acagcggcta taccaatcaa
    38041 agactttaat gggttgaagc aagcattgag ccaacgagtg gatcattttc atcaaattgg
    38101 ctgtcgatta gcagatcacg gtttgaatac ttatttttat gaaatagcag atgaaaaaat
    38161 actcgacaat ctgttaataa aagctttagc aggaaaaacg gatttccatt tatcagaaat
    38221 tgcacaattt gttacaggta ttcaattaca tttgatgtct gagtatacaa gatatggttg
    38281 gacgatgcaa atgcatttaa atgctttaag aaataattca agtatattaa aggaacaaat
    38341 tggagtagat gtcggtggtg attcgatggg ggatcaagct agtttgacac aaaatgttgt
    38401 agcattgttc gataccgctg aaaagagtgg gtgcttacca aaaacaattt tatattcatt
    38461 aaatccaact gattggcttc cactagcaac agcaatgcaa agcttccaag ggggtatgat
    38521 tcaaaaaatc caattgggat gtgcttggtg gttcaatgat acagctgaag gaatgcgtca
    38581 acagttgact acgatggcac aacaaagttt gttgtcgaat tttacaggta tgttgactga
    38641 ttctcgaagt tttctaagct atcctcgcca tgagtatttt cgtcgagtat tatgtcaatt
    38701 actaagcgag tgggttaaag caggccgaat tccagatgat gaaatgtatc ttagtcagat
    38761 agtggagaat atttgttatc gtaatgcgta taattatttt aatttttttg aaatcggaag
    38821 tagaaaataa tacttgaaat aaaagcttaa atttattctt tgtaagttag tacagctaga
    38881 caaagtatgt atccataaag gaaggagata gatatgaaaa aagtagaaat tttaagtaag
    38941 ttagaagcgg ctggagtcat tgcggttgta cgtgggaaaa ataaggatga ggccattaaa
    39001 gcaataaatt caattattgc gggtggaatt aaagggattg aactaacttt tacagttcct
    39061 aatgctgaag aaataatcaa agaaattgct aaaaaatatg ctgatcaaaa agatgtaata
    39121 atcggagccg gtacggtttt agatgcgttt actgctcgta tcgcaattat ggctggggca
    39181 gaatatatcg ttagtccatc ttttgatcga caaacagctg agatttgtaa tttatatcaa
    39241 attccttatt taccaggttg tatgacaatt actgagataa aggaggcact tactgctggc
    39301 gtagatatca ttaaactatt cccaggttct gcttttggtc catccattgt aaaagcctat
    39361 caagctccat taccacacgt aaatataatg cctactggcg gagtaagtat agagaactta
    39421 gaagattggt ttgaagtagg agttgttgca gttggtgtag gtgggaattt attggcttct
    39481 gttgaaaaag gtgattttga agaagtaagt cgagtagcta gtaaatatat agataagttt
    39541 tatgagataa agaattatta ggagaaaaaa caaataatac tctgaaatgt aaaaaataaa
    39601 tactgactct ttacgcttca gagtatagta cgtactttca tcagaatgaa aacgcttaac
    39661 aacagaaggg agagaattgt atggaaagaa aatttggtat aagagacaaa ataggatata
    39721 tgtttggaga ttttggaaac gattttactt tcatttttgc tagttctttt ttaatgatct
    39781 tttatacaaa agtattgggg ataagcggag ctgccgttgg aacattattt ttagtagcaa
    39841 gatttatcga tgcagttact gatgtaacta tggggcgtat tgttgataga tcgaaagcaa
    39901 ctaagcaggg taagtttaaa ccgtggattt taagaatgag tggtccagtc gctttagcaa
    39961 gttttttaat gtatcaaact tctatgattt cagcttctat gagcgttaag gtagtataca
    40021 tgtatgttac gtatatatta tgggggagta ttttttacac atctataaat attccttatg
    40081 gatcaatggc atcagctatt acttctatta cagaagaaag aacaatgcta tcgacattcc
    40141 gaaatgttgg agctacttta gcaagtttgg tcattggtgt attaacacca ttatttattt
    40201 atacaagaga tgattcaggc gctcaaatag ttcgtggtgg atcaactttt acaattgttg
    40261 cgggaatatt ttcgatatgt gcacttattt gttatttaat atgttataaa ctaactactg
    40321 aacgagtaag aattgaccaa tctcaataca aagtttcatt gttaaaatct ttaaaagggt
    40381 tagttaaaaa tagggcatta ctttctataa ttggtgcggc tattttctta ctccttgctc
//
LOCUS       DAYYAO010000017        39792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000017 DAYYAO010000000
VERSION     DAYYAO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.018"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..588)
                     /locus_tag="ACHARI_000810"
     CDS             complement(43..588)
                     /locus_tag="ACHARI_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8441878.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(600..1292)
                     /locus_tag="ACHARI_000811"
     CDS             complement(600..1292)
                     /locus_tag="ACHARI_000811"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8441879.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1311..1595)
                     /gene="macP"
                     /locus_tag="ACHARI_000812"
     CDS             complement(1311..1595)
                     /gene="macP"
                     /locus_tag="ACHARI_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8441880.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1608..2171)
                     /locus_tag="ACHARI_000813"
     CDS             complement(1608..2171)
                     /locus_tag="ACHARI_000813"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8441881.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2399..3061
                     /locus_tag="ACHARI_000814"
     CDS             2399..3061
                     /locus_tag="ACHARI_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8441882.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3185..4381
                     /locus_tag="ACHARI_000815"
     CDS             3185..4381
                     /locus_tag="ACHARI_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8441883.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4482..5858)
                     /locus_tag="ACHARI_000816"
     CDS             complement(4482..5858)
                     /locus_tag="ACHARI_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8441884.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5895..6761)
                     /locus_tag="ACHARI_000817"
     CDS             complement(5895..6761)
                     /locus_tag="ACHARI_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8441885.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGESFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6850..7875)
                     /locus_tag="ACHARI_000818"
     CDS             complement(6850..7875)
                     /locus_tag="ACHARI_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8441886.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7893..8567)
                     /locus_tag="ACHARI_000819"
     CDS             complement(7893..8567)
                     /locus_tag="ACHARI_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8441887.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8751..9644)
                     /locus_tag="ACHARI_000820"
     CDS             complement(8751..9644)
                     /locus_tag="ACHARI_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8441888.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9718..11415)
                     /locus_tag="ACHARI_000821"
     CDS             complement(9718..11415)
                     /locus_tag="ACHARI_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8441889.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11408..12720)
                     /locus_tag="ACHARI_000822"
                     /pseudo
     CDS             complement(11408..12720)
                     /locus_tag="ACHARI_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(12720..16661)
                     /locus_tag="ACHARI_000823"
     CDS             complement(12720..16661)
                     /locus_tag="ACHARI_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8441890.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16770..17228)
                     /locus_tag="ACHARI_000824"
     CDS             complement(16770..17228)
                     /locus_tag="ACHARI_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8441891.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17526..18044)
                     /locus_tag="ACHARI_000825"
     CDS             complement(17526..18044)
                     /locus_tag="ACHARI_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8441892.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18046..18663)
                     /locus_tag="ACHARI_000826"
     CDS             complement(18046..18663)
                     /locus_tag="ACHARI_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8441893.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18660..19514)
                     /locus_tag="ACHARI_000827"
     CDS             complement(18660..19514)
                     /locus_tag="ACHARI_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8441894.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19511..20422)
                     /locus_tag="ACHARI_000828"
     CDS             complement(19511..20422)
                     /locus_tag="ACHARI_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8441895.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20463..21797)
                     /locus_tag="ACHARI_000829"
     CDS             complement(20463..21797)
                     /locus_tag="ACHARI_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8441896.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22005..22835
                     /locus_tag="ACHARI_000830"
     CDS             22005..22835
                     /locus_tag="ACHARI_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8441897.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23079..23328)
                     /locus_tag="ACHARI_000831"
                     /pseudo
     CDS             complement(23079..23328)
                     /locus_tag="ACHARI_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23519..23713)
                     /locus_tag="ACHARI_000832"
     CDS             complement(23519..23713)
                     /locus_tag="ACHARI_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441898.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24218..24403)
                     /locus_tag="ACHARI_000833"
     CDS             complement(24218..24403)
                     /locus_tag="ACHARI_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441899.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25253..25594)
                     /locus_tag="ACHARI_000834"
     CDS             complement(25253..25594)
                     /locus_tag="ACHARI_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8441900.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25594..25824)
                     /locus_tag="ACHARI_000835"
     CDS             complement(25594..25824)
                     /locus_tag="ACHARI_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8441901.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26111..26449
                     /locus_tag="ACHARI_000836"
     CDS             26111..26449
                     /locus_tag="ACHARI_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441902.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26654..27216)
                     /locus_tag="ACHARI_000837"
                     /pseudo
     CDS             complement(26654..27216)
                     /locus_tag="ACHARI_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27948..28529)
                     /locus_tag="ACHARI_000838"
     CDS             complement(27948..28529)
                     /locus_tag="ACHARI_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8441903.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28712..29338)
                     /locus_tag="ACHARI_000839"
     CDS             complement(28712..29338)
                     /locus_tag="ACHARI_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8441904.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29360..30691)
                     /locus_tag="ACHARI_000840"
     CDS             complement(29360..30691)
                     /locus_tag="ACHARI_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8441905.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30846..31247)
                     /locus_tag="ACHARI_000841"
     CDS             complement(30846..31247)
                     /locus_tag="ACHARI_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8441906.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31333..31539)
                     /locus_tag="ACHARI_000842"
     CDS             complement(31333..31539)
                     /locus_tag="ACHARI_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441907.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31634..33136)
                     /locus_tag="ACHARI_000843"
     CDS             complement(31634..33136)
                     /locus_tag="ACHARI_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8441908.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33154..33609)
                     /gene="argR"
                     /locus_tag="ACHARI_000844"
     CDS             complement(33154..33609)
                     /gene="argR"
                     /locus_tag="ACHARI_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8441909.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33707..34324)
                     /locus_tag="ACHARI_000845"
     CDS             complement(33707..34324)
                     /locus_tag="ACHARI_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8441910.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34545..35918
                     /locus_tag="ACHARI_000846"
     CDS             34545..35918
                     /locus_tag="ACHARI_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8441911.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35993..37105
                     /locus_tag="ACHARI_000847"
     CDS             35993..37105
                     /locus_tag="ACHARI_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8441912.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37177..38814)
                     /locus_tag="ACHARI_000848"
     CDS             complement(37177..38814)
                     /locus_tag="ACHARI_000848"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8441913.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38982..39590)
                     /locus_tag="ACHARI_000849"
     CDS             complement(38982..39590)
                     /locus_tag="ACHARI_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8441914.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11438 a         8452 c         6902 g        13000 t
ORIGIN      
        1 tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa tttctgcgcc
       61 tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga aacttgccac
      121 ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg ctgtatgcaa
      181 cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc gttctcgcaa
      241 acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta tccaataaac
      301 attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca attcccgtcc
      361 acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa aggaatcttt
      421 tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa acttctttgt
      481 atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca gctttccatc
      541 ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct tagtcctcct
      601 tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg
      661 ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg
      721 atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc ttccatttcg
      781 caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg cgagtcgaca
      841 aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc ttttgctgct
      901 ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat tcctggtaat
      961 tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac tttgtctgat
     1021 ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc tgtattgatc
     1081 accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc taagactttt
     1141 cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga aataaataaa
     1201 gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat ttttacttct
     1261 tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt ctatataaat
     1321 gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga tttcatcaaa
     1381 aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt gattggttta
     1441 ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg ctgataagct
     1501 gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga
     1561 agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta ctttcctttg
     1621 gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat caactgatct
     1681 ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac ttcgtcttca
     1741 tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat atgaaggact
     1801 tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga cttcgcacga
     1861 tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg tccttctcct
     1921 ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt tcgaaattgt
     1981 ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc gccaggatga
     2041 aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc tacagcaaca
     2101 tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc aaattgctca
     2161 ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt ttcatttttc
     2221 aatttttctc aaaaactcct tattagttta ccatagataa accttagatt ttctctaaat
     2281 atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta aaataagtat
     2341 gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag ggtttattat
     2401 gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct ttgtccgaaa
     2461 caatagtttt tcttttgttc taggactttt attgataggt gtagttttag ttttgtgggg
     2521 actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac tgactgacga
     2581 acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat tttttcgaca
     2641 aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc agcagacagc
     2701 caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag ctttattcaa
     2761 agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt atacgcatct
     2821 gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg agatcaaaaa
     2881 caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt
     2941 aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg acgtagagat
     3001 atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac aaaaggatta
     3061 actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct gtttcgagta
     3121 ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca aggaggaatt
     3181 ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg atacacttga
     3241 agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt cacaacaagc
     3301 ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca aactaccggc
     3361 ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg atgcacagtc
     3421 tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac aatcgatgtt
     3481 gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg aactgatgta
     3541 tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca tcttccagcg
     3601 tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg
     3661 tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc tgctaaaaga
     3721 taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg cactgaatat
     3781 ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca ttccagaagc
     3841 gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca atgattacac
     3901 gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta gacagatcac
     3961 gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa
     4021 gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt
     4081 gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg aaacaacaaa
     4141 cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga
     4201 aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg atttgatcga
     4261 aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga
     4321 agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt taacccaata
     4381 gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac aactgttgat
     4441 gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc attcatcttg
     4501 ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac gataccgaac
     4561 acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa aatggagacg
     4621 attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt gccaaacatt
     4681 tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc catgatattc
     4741 gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc tgccacacca
     4801 gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt cataaactta
     4861 ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata catcatcgag
     4921 atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc cactgttcca
     4981 tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc agtatacgga
     5041 acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa
     5101 ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc tgccattgca
     5161 acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag cacaccggaa
     5221 aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg
     5281 ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa acgtaatgct
     5341 ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac cgcaatcaag
     5401 tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg aagttcggcc
     5461 cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac agaggaaacc
     5521 gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat
     5581 acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc aaattctgcg
     5641 aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag tgacagtgct
     5701 ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt accgatgatc
     5761 gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat ctcactcgta
     5821 tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc ccattttagg
     5881 ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc taaaatgact
     5941 aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc gccaaggatg
     6001 aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa gccaacagct
     6061 aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca taataatcca
     6121 cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc aaagaagctt
     6181 tcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc caaattcgcc
     6241 cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa tgctctaaaa
     6301 cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc atcttgtcgg
     6361 gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta tacataatct
     6421 ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa aattaattga
     6481 aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc atggaactgt
     6541 ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa aatccaaggg
     6601 gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc accgatcgtc
     6661 attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa accaatacta
     6721 ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct ccttttattt
     6781 cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt tttcgtttct
     6841 aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata aagtcattgt
     6901 tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt cggccagatt
     6961 ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac catttctccc
     7021 agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga tccctgaatc
     7081 tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga tgtttccgtg
     7141 atccggcgta tatttgatgg cattactaag caattggttg aagataaaag cgatccattt
     7201 agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat ttttttggat
     7261 aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc catattcttg
     7321 gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt gttctacgta
     7381 ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt caatatcgaa
     7441 ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt catggaccca
     7501 tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca ttacttcttg
     7561 gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt ctgtcctccc
     7621 accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt ttccccacca
     7681 tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc cctcaatcaa
     7741 agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc acatcacaaa
     7801 gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat ctttcaaata
     7861 tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc ctgtcctact
     7921 tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa ccgattgatg
     7981 ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc tcgcagcaat
     8041 ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag acgatattca
     8101 tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc aacattcaac
     8161 gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta agaacggcga
     8221 agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt cacaaaatca
     8281 tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct ggagataaaa
     8341 atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc atccaaaacg
     8401 ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc tgagaacact
     8461 tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc ctccatgatc
     8521 aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa aaatccctcc
     8581 atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt ggcttacctg
     8641 gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta atcgcgtttg
     8701 tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta ttatctgtta
     8761 tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc gaaaagcgtt
     8821 agcactagca atatacctag aaggagtata agtaatcgat aaagatggta tttttttgtc
     8881 tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt cactagtaat
     8941 cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc ctgcttttct
     9001 ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac gacttcataa
     9061 gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat ttcttcaaga
     9121 tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga atgtgtactc
     9181 tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc taacaaaaga
     9241 tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata gatggctccg
     9301 atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac cgcttgctca
     9361 aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag cgtctgattt
     9421 ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg ttcaagctgc
     9481 ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc tgctaaaaat
     9541 ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa ttgtaataaa
     9601 agactgattt gggtttgagg tctattacct attttctttt tcattttctt caccttctta
     9661 tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt ttgttgctta
     9721 tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca tcaaacttat
     9781 tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg acccgcgttt
     9841 catattcggt actctttctg gccctggatt ttctactcct acgatctgaa tttcttcatt
     9901 atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg gaagtacata
     9961 accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc cttgaacctc
    10021 aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg cccactcaat
    10081 tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa atattgcgta
    10141 ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt cactgcttga
    10201 tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg gctctactcc
    10261 ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt tgttatggaa
    10321 atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta aatctcctcc
    10381 acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt cttctgtcaa
    10441 aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc cttttgttaa
    10501 aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac caactgtagg
    10561 cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga cagaatcgat
    10621 gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt ctagatcaat
    10681 ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc taaggtcgct
    10741 ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat acaaatgcac
    10801 atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca ccatatcagg
    10861 catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat gagctggcga
    10921 ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt ccgtcacttg
    10981 gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa tacttgtcag
    11041 tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa tcgcttcggc
    11101 agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca aatcgtaata
    11161 tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt caccaaaatc
    11221 cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac cttttttgtg
    11281 taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga ctagttttcc
    11341 gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc ccgtgacaat
    11401 cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca gcacctaaca
    11461 ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc gggaatacgg
    11521 accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt ttgtccgttg
    11581 tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca gagactggaa
    11641 ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat tgtggttggc
    11701 cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt gcttcatcca
    11761 tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa ggcaagaaac
    11821 gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca tttttgtcat
    11881 tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta gcaggagcca
    11941 tatctaaata atatttcccc cttctatcat ctgatataga gcaaaaacag caccttctaa
    12001 tggttttcct ttttcagttg ttcctctttg acgtccatac ttgaagaagt atggatctct
    12061 tagataaccg acatttttag gatagatatg aatctcttgc agttcctcgg ttgattctgt
    12121 cgggtgataa attggcaaaa tagcagcaat tgggatagct gtctgatcga tatcaacatc
    12181 caatccaact tctggatcaa tcgattcttc gaaaatcaaa taaactgcat cccgatcttg
    12241 gtttttctta ggtgctttga tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt
    12301 cccagttgtt accgaagtcc ctatttggac aagcctttct tctgtgatta gctgacggat
    12361 attgtttctg ttcgtttcag cgatttgctg gacgaattct ttaggagtca tgttttcagc
    12421 agcttttttt tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag
    12481 tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct tcctcagaga tagccaagcc
    12541 atcattttca tggtattcaa attgatcgtt ataatcgata tctcgaatca tccgtttatg
    12601 caacacgaat tccacgacat cttcccctga ttcttctgcc gacatcgcat gtggaagaag
    12661 gactgctgca agcggtagta aaacaagtaa ttgaagcatc aaacacaaca gctttttcat
    12721 taccttgctc cctccttttt agattgtctg acaaacaaac cataggctgc tcctgcaagc
    12781 gccatgaaga atgcagcaag caggatatag aataatcgtc cggatcctcc tgtagcaggc
    12841 aacggatttt caggatcgtt cccgacacta agctgtaatc tgattcgatt tgtctccaaa
    12901 agttcatatt ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt tggataatac
    12961 gaaatctcaa cttttagtgt acccgcttct tttcctggaa ctacccgatc attttctatg
    13021 tcaggttctt ctcgataact aatttctaaa ataaagtaac ctggaagttt tctatagctg
    13081 tcaggtgact tcacttcctt gatccgatag accccttcca acaagtctga agcattgaat
    13141 agggaagaat cgtttggatc tggtgccaag cctccctcga tgatttcata ctctcctgca
    13201 ctattttgtt tctcaattga aaattctgcg ccttcaagac gtcggctatt catactgtca
    13261 attttcaaaa gttccaaatc gattggcgct agttcatctt ctaaggttac ttgtagtaca
    13321 ccatcgatga tttcatagcc ttccggcaag ggagcagttt cgtcttcgat gaccgtatct
    13381 tcaatgataa acttaccttc ttcattcagt gcaaactgga acacttgatc agatagccga
    13441 tacccatttg gtgcgacaga ttccgagacc tcatattcac caatagacag atgcagaggt
    13501 tggtcgtaat ttaaatcgac atacttttct actcctacgg cacgagaata gaagtatccg
    13561 atcccaggca aacgtttcga ttcttctggt tctaacggat taccagtgta tgctcgatac
    13621 agcgggaagg aagaatcccc cactttcctg atatcgaaac ctgcaccatc taatgtatgg
    13681 ccttcatggt ctaattttct aactcggatc tcccgataat caaacgtatt gtaagcagtg
    13741 aatgctaccc ataaacgatc ctcttcatct gttgtatacg tgagatattg ccaattttct
    13801 tcacctgttt gcttgagttg gatagtccct atagcattat cgtaaagtcg caagatccat
    13861 gtgtcatttt catcaggttc tttgaaacct tctgggttct ggctctcaat gattttgtat
    13921 tctgcatagt tggtagtttc ttcactatcc ttataaacaa atgaatctgg ctctccgatt
    13981 ggatgaataa ctttctcacc atccacgatt acatatttcc cgtcttcatc tactgaatag
    14041 aacgtgagtc tccctttctc ttcctctact tcgttcacac cagaaagccc tttcgctact
    14101 ggatatccga tttccagtcc gttaccatct gtttcataaa gttcaaactc tgcgccattg
    14161 attggcatat tactgccttc aattagttta ttgatattca tgtggattgg cttgaattca
    14221 tttcgcattt ccagtttgac atacaactca tcatactctg tgccttctat gttaggctct
    14281 tgtacaacag aaagcttgtg atattctcct tcataatagg aattcggatc tttaccttcc
    14341 ggtccgccga ctctcgaacc agatgtatat tcatctacaa gctcgtagtg acctaacatc
    14401 tcatcttcgt tggctgcata ttttttgatt tggaattgat acgtctcatc tactaactga
    14461 taacgtggtg gtccagcgat ctctctgatc caaaatatgt cgctgactgc tccagtttca
    14521 tctcctaaaa tcggcatctt cgtttcaaag atcgctattc cgtccgtggt agttttactt
    14581 gtttcgattg gttcggcttc cccactctct ggattttggt catataatgc aaattcgact
    14641 ccagccaacg gctcgccatt ttgacccttc ttgctgactt ggaaattacg gcctttatgt
    14701 tcgtaacgaa tggaagaaga tgggaaatac atccgtccag tagtgatatc attctcataa
    14761 tcctccaggt tctgaatata ggccatattg ttcactgtgc aaaaatttcc gtcttgagct
    14821 cccgcatcaa gttttacttg gtacttgatc ctgaccattt ccccttcacc taaagaaatc
    14881 ggagaaaatt tataagcttg gttttcccgt ccgacatacg ttgtcggagt cgccactact
    14941 tctggtgggg cttgtccatg acgttcttca tcccatattt ctggctcttg gtacttcatg
    15001 ccgttttctc ctggatctag atggtaaaac tgaatatctg ccgatctgcc agctccaaat
    15061 ggtgagacat taaaaccagt ttcatcataa agtgtggctt gctggatcgt tctcttgaag
    15121 gtagaagaga tatcctccaa ggctgtaaca atgtcattct tgttgttcgc attgtaatat
    15181 cgcggtgtta cgccatcttc ttctaaatca gcaatatttt tcaatacatt tcgtccaata
    15241 gcatctagtc gttgtctccc gctaacgttt ccatcgattc ctaaaccaat ggagaagata
    15301 cttggcgggt tctcttgata cttgtttttc agccaatgat gataagcaac tgtgttgatc
    15361 gcagatgact tttcttgcga agtaggactg ccattcgtat tgttattaga agaaccatca
    15421 ccaacccagt gagtcgcttt attatctcct tcttttacct ctccataacg atagtgtaca
    15481 tttacttcat cttctggacg gttaaagtca gatgcatagg taactggatg agtaaaccct
    15541 gcattagaag tattcccaga aaactcctca aaatttctga acaaaacgtc attatcttca
    15601 gcaatcttat cttccatcac agtccagttg ctccaagctc cattagtgct cgtacgataa
    15661 cttgactgaa tcggtgcata actgaaggtc ggtgtcccat ctcccacaac gattaggatt
    15721 ttttctggat ttctattttc tccgccattg tctttataca atgtttcata gccattttta
    15781 agccctagtg taagcggagt tcctccgatt ggtgtacgat ttaatgctgt attctcactt
    15841 cccgtcaaac gattgatatc atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg
    15901 ttattgatag ctgtactatt atggtaaaaa ttcaccatac cgattcgaat atccagactc
    15961 gggtcagaga gcagatcttt actcatttcg tttactgcct caatcaacgc agcattttta
    16021 gtttgactat tggtcccagc ggttaattca ctcatactcg ctgacttatc tagaacaaaa
    16081 acgatatcga ttggactgat ttcctttttc tcgcccccga tgacatctag atagacattg
    16141 taaagggttg ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg cagaacaggt
    16201 tctacccatt ttttgatgta cgccccgtta taatccaagt aactgttttc aaaattccgt
    16261 tcatttcccc aaagctgaac ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt
    16321 gatccattat tagtagcata gtcgtaattt cgccagttcg ctcccccgtt tgtataatca
    16381 ctatcatcga tcaagttctc tccgtcatat ggcgagcctt tgtctccttg atagcgttca
    16441 ttgctttgtg tccaatttcc taacactttt gtatagtcat tcgttgtaaa cttcgggtag
    16501 cgatgctgtt cgttttcaac ttctccgcta ggatagatat attcataggg gtcttgcgta
    16561 gctgttggcc caagacgctc caaaataccg ttgaataact ggcgttgaat gcgctggagt
    16621 gcttgaccgc tgggtacgat tggttcgtcc agcttttcca tctcattttc ttcgctacgt
    16681 tgaatttcag cggaatcttc tgtatcagtt tcattttctt gtaattcttg gttatctatt
    16741 ctttcatcaa ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct
    16801 gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat
    16861 tcttgaccat cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga
    16921 cctgttccac gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa
    16981 cgtattctac cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt
    17041 tcttcttttt caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca
    17101 tcctcctttg tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac
    17161 tggaaatatg gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac
    17221 cctttcacat ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa
    17281 acaatgccca tctaattata atttaattta attttaattt taaatcaaat gatatccttt
    17341 ttagttaaaa aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat
    17401 aacgttctca tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc
    17461 ctcgaaacaa aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa
    17521 ttaattcaga taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct
    17581 aaaatgtcag taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca
    17641 aataaaaagg ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa
    17701 aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg
    17761 acaagccgac gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc
    17821 aaaccaacaa aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta
    17881 tcaggtatca agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata
    17941 ttactgataa atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac
    18001 ttgttgataa tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt
    18061 aagaaccttt tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact
    18121 gaaacctaat aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa
    18181 cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt
    18241 aatccaagaa gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc
    18301 gcttaataga agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca
    18361 atagcggaaa atctgataat cgatcaatag aagaagaaaa taaacacttg ctaaactatc
    18421 ttgctttcca taaagagaga agttctcacg aaactttttc cagaatagtg aaccgagctt
    18481 tttctcttca ttttttttgt aatgataaat catatacaga actgttcgac ttgcagttcc
    18541 taatgtgaaa acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg
    18601 aatccattca atgatttcta agaaataatc attggctttt ttaccaatcg atcgattcat
    18661 catccttttg aacctcctgt caaatccatc ccttcaatga aataacgttg tgcaaaaaag
    18721 aacagcaaaa tcgtcgggat caagacaagt gttgctcctg ccatcagata attccattgt
    18781 gtcgttgctt ggttctgaaa aacttgcaag ccaatctgta acgtgtattt ttcttgatct
    18841 tggatatata acagaggacc taagaaatca ttccaagctg cgttgaagga attgatcgca
    18901 atggtaatca aggcaggttt cgtcaatgga agcatcaaat gactccaaat atacaaatga
    18961 ttcgctccgt cgatttcagc agcttcgatc aattcattat tgatcgataa gtaaaattga
    19021 cgcatcagaa atatattgaa cgcattgccg aagaacgatg gaacgatcag gggcaaataa
    19081 gtacctaccc agccgatctt ggagaataat acatattgag ggatcatcgt tacaaacccc
    19141 gggatcatca tcgtagaaag aaccaaagca aacatcagct ttttcccagg aaaatcaagc
    19201 ttcgcaaatc cataagcaat aaaagaattt gaaaggacat taccgatcac tactagcaca
    19261 gtgatgaata atgtatttct tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga
    19321 tagttttccc atacgatttt ttctgggaaa aaggtcggag gataacgaac gatttcttcc
    19381 attggtttca atgaagtaaa caccatccaa agcaatggaa tgatcaaaat gattcctatt
    19441 actgtcatga ggacataact tagtattttt tttgtttgcc gattcacact ggtctttttg
    19501 aaataatcca tcatttgccg tcacctccag aataatagac ccacttaggt gcaagcttca
    19561 aattgacaaa cgtcagaacc aacacgatca gaaacaggat cattgacata gccatcgcat
    19621 aacccatatc aaaagctacg aacgccttgt tccacatatg cagattataa aaaagcaaag
    19681 aatttgcagg tccgcctgtt ccatttttcg tcatgacata agcttcttga aaaatttgga
    19741 aagaaccgat cgtagaggtg atcacatcgt aaaaaataat aggagtgatt aatggcaaag
    19801 taatactttt gaactttccc caaacgcctg ctccatcgat ttctgctgat tcatataaag
    19861 atctaggaac attttgtaat gtagctaagt aaagtagcat tgctcctcca gaactccata
    19921 ttttcatcaa aatcaacgca ggtttcgtcc attgtggatc aaacagccaa gctggtccag
    19981 ggatatggaa ccagcccaga aatgtgttga tcaatcctgc agatggagaa agcagctgca
    20041 tccataagat gtagactgct actcctgata agactgccgg taaatagaaa gctgtacgga
    20101 atacactgat tcctttgatt ttttgattga gtactgtggc taaaaacaca ctgaaaatcg
    20161 tcgtcagcgg tacattgaag atgacgaaat aaacggtgtt gtataacgat ttccaaaata
    20221 aatcatcgtg aacgaacatt cttttgaaat ttgctagacc aatgaaattc atcttggatg
    20281 tcaaattata atcggtgaaa ctaccaataa ctgaaaaaag cataggccct agcataaata
    20341 ccaagaaccc gataataaaa ggcgagataa ataaataggc ccatcttgct tcttttttct
    20401 ttcggataga taaacgcttc aaattttctt gcctcctctg tacctagaca gtcaaaaatg
    20461 gattattgat tttgcttgac taagttttct actgcttttt gtgcatctgc caatccttct
    20521 tttggtgatt ttgttcctaa aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa
    20581 tccggagcat ctaaaggaac tggtgtgatc accgtatttt taaagttttc gtccgccatt
    20641 tgatagatca tttttccttt atccgttagg ttgtcattat tcactaaatt ctcattggct
    20701 gtcttgctcg ccatgatatc aaaacttttt tcgccgaatt tttcttgtac ttctggagaa
    20761 gaaagatatt tgatgaattc ataagaggcc tcaggatctt ttgctccgta tggtacttcc
    20821 aagacgaatc cgccacccca cgaccaatgg cctgaacctt cttctttttc tgggagctgc
    20881 atcacgccaa aatcaaaatc atccggtgca ttttccatca aactagagta ataattgata
    20941 ttttgggcac gcatggctac taatccggaa ataaatggat cagatacacc agacccaaat
    21001 tcggcttcta atttattgat cgtatttttc ccataataat cttgccagtc ttggatccac
    21061 tccaaggctt ccactttatt tttagtatca atcttgactt tttcatcttt gtcaaaccaa
    21121 ctagtcccgt tatctgcgtt tagcgcccat acatcagccc ctaaattcca taatggataa
    21181 aaaccgatgc gtgtaaattc ttttccttct tttttatcta gtttacgtgc tgtttcttct
    21241 aattcatccc aagttgtcgg tagatcttgt tcactgattc cagcttcttt aaataacttt
    21301 ttattataaa aaatgacttg tgtatctgta ttgaatggga tcgcataagg atcaccttca
    21361 tattctactg tatcccaaag ctgcggataa aaatccttag aagtgtcttt ttctacatat
    21421 ttggagagat tagttgcttg ctgtgcttct gctctttggg caacggaatt tatgtcctgt
    21481 acaataacat cgggtgcatt tccagctgtt attgcagata aagatttagt ccagatgtca
    21541 ccccatggct gatacttata atttacatgg atctcgtttt gtgattgatt gaaatcctca
    21601 atgatttctt cgatagcttc ttttcgtgca ccggatcccc aaaaggacca gaaatcaata
    21661 ttttttcttc catcacttgt tttttcagaa gaatttgctg cacttgaact ggcatcgctt
    21721 gcattacttg atccgcatcc tcctaataaa aacaatgctg aacttgccag caaccctgta
    21781 aacaataatt ttttcatatt ttacctccaa attttaatgt aaccgatgcc ggttttaata
    21841 taacgatatc attttgttct gaaaataaca atttataagg tatgattcta aaaaaattat
    21901 taatgatacc aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg
    21961 tagcagaatg agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga
    22021 aagaattgca gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc
    22081 aggagctgat cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag
    22141 gctggagctg ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag
    22201 aaatcggcca cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg
    22261 ttgaagatca agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa
    22321 tgaatccaca gcacgcgatc gtagctggct taggacctcg tcctgctgac agcgtgggca
    22381 atccatggag cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta
    22441 atttaaatgc cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg
    22501 atcgtggcgt aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc
    22561 tatgggaatg tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta
    22621 ctttcccgcg tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg
    22681 gagaccaaag tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat
    22741 atcaatggtt gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac
    22801 caaaaatgta tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt
    22861 ttttgttcca cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc
    22921 aaaagggtgg ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct
    22981 ggctttcata ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa
    23041 ggtttatgta ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta
    23101 tctaacgcac tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt
    23161 tgtcctactt ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat
    23221 tttagatttg cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat
    23281 tatttttctt ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg
    23341 ggctaaacta tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg
    23401 ctcggcgcgc tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca
    23461 gaaacaacgg gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt
    23521 atttttttga ttttttataa atggaaaatg cttcaagaag tccaagaaat aaagccgtat
    23581 aaaaacaaga acctatgaaa tctttattta ttagatattc aacagaaatc ccgataacac
    23641 tagccaacaa tatatttatc agaagtgtaa aataaggatg ctttttatag aatagaacta
    23701 gtgttgtttt catagactct cctttcttca tatttccaat taatcacaaa ttcacggcat
    23761 tactagaaaa attaaagggg cgcagaagtg gaaaaatact cttcgacttt ccctacaaac
    23821 caggccatct tgtgtttaat aactatacca aatctaaggt acctctaaat ctaaattcca
    23881 tttgtggcgg tgacctccaa tttttaactc ttctatctcc catccagtat acataacctt
    23941 gatacttcga ccacttatat atcgaacaca caaataccta tccattgcag gagtactacc
    24001 taacttagtt gctacttgca tctaaataac agctgaaaag ataggactta ctcttagtaa
    24061 agtatctgaa ctattaattt ttaaactatc atttttatcc tcaactacat gcacatctgt
    24121 tgcaacttca ctttctgtct catccagata aatgattaag gaatcgggaa ttgcaatatc
    24181 ttcagctgac gctggaatag ttgagctaaa ttagtcatca tatctttatt ataaaataac
    24241 caacagcaaa aaatacaatg aaaaacaaac ttggaactaa tgtatcatta ataaatgttg
    24301 aaaaattcag caactcatta ctgtatgatt ttagcagccc aaccaacata taccctataa
    24361 aagctaataa gatggttagg aatcctttag aatttttact cattcataga cacttcctta
    24421 aataactttt attatagaag gtattgtaaa taataatatt gacaaaaagt gactcaaaat
    24481 aaaaaaaacc ttacattata ctggaatgaa ctgtattgat agtgtaccta aaagctctac
    24541 tgacataaga tcgtgtaatc ggtaaagtaa ttttattaat tatacgggat gtaatcaatt
    24601 agagaataaa ctaatcataa aaggtacttt tacattcata attgtcgctt tgagattcta
    24661 ctttggttca ctatctgacc caattgcaca ttttcgatca ttacaagtat tggtgtcgtt
    24721 agaacaacta aataaaaact agcggtagat ttttcagaat ctatgctaat ttctgtatgg
    24781 atgttccagt gatggcaaat ttctcttttc attgtttttc ttgtcgcatg atatgccttt
    24841 ttgtgttatt cagttaaact atagaaattc tttcctagac agatcttgat cccaaatttt
    24901 acgtttttga gcatcgatat agtttaaaat gacattactc atatctaaag agtttagatt
    24961 ttttaggact aaaactgtca gttgttcttt ttcttctttc tgttaaagta ttgtcgcgac
    25021 ttttcgtata cgttaagata tgtcgtgaaa aaacaagttt tgtgacacaa aaaaacgaat
    25081 gacttgagga aatcacctca atcattcgtt taaaatgcta tatattcttt agtttaagat
    25141 acggaaatta atgccatcga ccacactagt agaatgaacg acatttactt ggcctttttt
    25201 taatttcgtt atgtttagca tataaaaata ttatagagtt tacttacaat gcttaaaaca
    25261 tatactgtat aatctgatca attttagcca tgtcttgcaa tggtaagttt tctatttttt
    25321 tgagttttcg ttccttaaaa tcaagagatt ttaattgtgt aataattact tgtcctttta
    25381 tatctagctc ttcaggtaat gagtaacgtg tcggtaaatt ctttatagtg gaagtaattg
    25441 gacatatgac cgaaaacatt gttttacggt taaattcata acgagagacg actagtcctg
    25501 gtcgtctttt ttgaatttct tttcctacgg agggatcaaa atcaatccat acaatatcac
    25561 cttttttagg aatataaatt ctttcttctg acactataaa atttctctcc cttcaggagt
    25621 taaatcttcc cattcgtctt tctcataata ctcggcctct tccccaccac taaatggatc
    25681 ttcaattttt ggaactaacg taattgttcc atcatcagaa tacacaacga tatattcttg
    25741 gttttctgaa ggtttctttc cgttatcaga aggtaaagtt accactacag aacttccttg
    25801 taatctagat tttgtaacaa gcattatcat cactccctta ataaaagtat actatataac
    25861 actaagtaac gcaaaattcc cctttgtaaa ctatctagaa aattgttttg tttaaagtat
    25921 atcttattgg atataaatag aaataaattg tagccagttc attttttctt taactaaaaa
    25981 aataatccaa gaaacattga acctatggat ccaaaagcta cccaaatact atagctaacc
    26041 ccccgctacc ttcaattttg cagataaaac gctttgggag aaaatttcaa agacaacagg
    26101 ccatcctatc atgcccgatt ataaacaaaa agctggctat tccttttgct ctgacaaatt
    26161 tcaattggga aagttcacga aactagcagg tgaagaagtt tcaactgttc ggatcaaaga
    26221 atgcccgctt caaatagaaa cattggttca acgaatttca actagacaag actttgctat
    26281 tgtagaacgt gagatcgtag ctatttttgt agaagaaggg attttacatg atccaactca
    26341 tatcaatgtg gataaatggc aacctcttat ctataaattc agagaatata caactgccaa
    26401 ccagtcgtta actttgaatt ttagattcca agaattcaaa gagtcataat gataaacatc
    26461 gttaggtata aataaaaaag actcgcagca cgagtaaaaa acaaatagaa gctatacatt
    26521 agaaaggtcg actgtgaccc tatagatgca gtatatgcag cttatgagca tttcttctgt
    26581 tctctttgtt tttgggatct gtgctgaagt ctttttcgtt ttttgccgct ctttctttga
    26641 aaaatcgatt atgttatacc ttctccatta ctaattcagg catctgctga gtaatgattt
    26701 ttcgcgcaaa agaaatatct gttacccaag gttcgatttg ttcttcagtc aagacaagcg
    26761 gcattcggtc atggattggt gcgactgtgc ggttaggttc agtagtcatt atgattgatt
    26821 ctgccatttt ttcatcctga tggctacgat aaaaaccgcc aatatacact attttttcct
    26881 tatttgaaaa cataaatttg tctttttctt cattccactc attgggaaaa cacaacgatt
    26941 ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat
    27001 catcaattgt ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat
    27061 gttttcttcc tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc
    27121 taattctgct gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag
    27181 ctctgactct agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt
    27241 ctatcataca ctgcaacaag aagttcgcac caacaaaacg ctttcaattt ttgtagaact
    27301 aaaaaagttg tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg
    27361 gtcacaagtc acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga
    27421 ctcggagtga aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct
    27481 cggtattaag ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa tttttcttaa
    27541 tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca
    27601 tctgcggtaa taagcccaac caaacgaaaa atatgagcag ctatttttat ttggctgttc
    27661 ttattacttg atgcagggca gctttttcac aacctctcca ctaaacggtg ttcaatcagc
    27721 taaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt
    27781 ctctaatact caggtcaaag cgctgatttc acaacctcta atacacggtg ttccagaagt
    27841 agcttcttcg aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt
    27901 ccttcttatt tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt
    27961 tgcgaagtta acaatctttc tttgatcaat tgaaaattct gcctaagtag tgatagatcc
    28021 tgtgtgactt tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga
    28081 cggtctgctt cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta
    28141 tacttgaccc attgattgac taattctagt agcaacagac ggtattcttc atccactgta
    28201 ctcaactcaa taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga
    28261 tcagcgaaaa tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt
    28321 aagatgtctt cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga
    28381 caaacttctt tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct
    28441 gctagaatat ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc
    28501 gcttccaaga tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc
    28561 tatattatac agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa
    28621 attatctgac acagccctca ctataaaact gttctcatat agctagatgt tcattgattc
    28681 aagaactgcg aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg
    28741 ttgtcatcac ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt
    28801 ggccaggagc accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt
    28861 cacgcatatg ggaatcgcta cacaagtttc cgttcattcc accaagaatt actgtgtcga
    28921 ttccattttt tctcaattgg agcgccaaat cattgctttc tggtccaaag attttatgag
    28981 gagagctgat gatcgtattt tcatccaaat aaggtttcaa ttcttcgata aaatcggctc
    29041 ctgaaccttc ttccactgat tcatattgac tttttctagc aaacatctgc tgctcaagca
    29101 tcatatgttc tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag
    29161 gagaaataaa gagtttgtat cctttttctt ttgcttgttt taccatttca atcatatttt
    29221 cgatcgtatt gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt
    29281 tcaagaactc gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg
    29341 tggattctcc attcgttatt tattttaata catcatttag agcttcactc attgtcggat
    29401 gagaatagat ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa
    29461 tcaagttgat gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag
    29521 tttcagggtc gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag
    29581 ggacaccgct tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt
    29641 tttctgttaa acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt
    29701 tccggttatt cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat
    29761 catctagaga gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt
    29821 tgtctgcatc tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa
    29881 catctgtatt ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa
    29941 cttttgacgc ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg
    30001 attcttggtc tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg
    30061 tcatgtcttc taaaatcatt tgcgcgatat cttcatcttc acgtggaaga aattcagaat
    30121 gggcatcaag aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc
    30181 caatatatcc agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt
    30241 gcgtagaatc aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt
    30301 cagaaccagt attgatgaaa atccgttcgc cttcaacaat cactttttca ccattattcg
    30361 tcacttcgat ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg
    30421 cctcatcgct gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc
    30481 gagcaacagc ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag
    30541 gaatacagcc gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta
    30601 aaaccgattc accttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac
    30661 cgatcacaat attttgatat ttttccatca tttttccctc gcttcttttt tacctaccga
    30721 ttggtacaaa aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt
    30781 caaaaaaaac cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta
    30841 ttcttctaaa caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg
    30901 cagaattcat gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata
    30961 tgcacttcta atactacacc gatctgatag ccgccatcat ctaaattata aagagagaca
    31021 cgtgctttta ctgtggactc tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc
    31081 ccattgaaac aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca
    31141 tggattcctg gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca
    31201 ttgcgcccgc catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt
    31261 tcgattgacc atttgctact tggctataat tatagtgtat gacaagtaag gcgctttcta
    31321 caaaaattct gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa
    31381 aaaaatacta ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc
    31441 tacataatct tttatatgat tgattttaag atacatatat accagaaagt tgaccccata
    31501 aaccaatact gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc
    31561 tccctaccag cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt
    31621 caaaaagact ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt
    31681 ttgctaattt tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata
    31741 ctagtaacgg ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt
    31801 ctagatcata tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt
    31861 ccaacagtcg cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga
    31921 acaattttaa tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat
    31981 ctaatgtgat accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat
    32041 atgcaccatc atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg
    32101 ctagctgtgt atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat
    32161 agccggaaat ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag
    32221 aagaagtagg ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac
    32281 ttaatactgc gatcaaatta tttcgattga tcactgttcc agaagcactg acaactgcag
    32341 aacgatccac atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct
    32401 gcttgatact ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc
    32461 catctggttc caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt
    32521 cagcaaagaa tccaccagca ccatttccgg catcaacaat gattttccaa ccatttagtg
    32581 gtttgctttc tagtgttcca atcccctttt taatttttgt gactaaatct tgcgcataag
    32641 gtgtcaaaag gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct
    32701 gtgataggat aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta
    32761 ttccattata ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga
    32821 actgttcaaa ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga
    32881 catgtatccc ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta
    32941 tcctactgtc atgaccaatc ccaatcgtcc attttttatt tggatcggtt gatttcttct
    33001 ttttctttat ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt
    33061 ttacttcata tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg
    33121 ctttcatatc tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc
    33181 tgcaaatcgc tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc
    33241 catcgttccg acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc
    33301 accatttcgt tcagtcaata cgataaccat gaactgaaca gactggatac gcaaaacctc
    33361 gcgtctaatt gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa
    33421 tgtatagcgt actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc
    33481 tcgagaaatc gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc
    33541 ttcctgtgtc ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt
    33601 ctttttcata ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc
    33661 atattttggt attttttact tttatttttt gttttcattc aggttgctaa cctttttcta
    33721 ttttaacacg caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt
    33781 aaatctcttt gtgttcttta gaagctcttt catagccttc ttttgcgatg aatgcttcta
    33841 atttctcaaa cgttgctggt tcttcggcat agggcccgac gtgtaagatt tgggcacaaa
    33901 gaccttcttc catctttttg aatactactt gttctttcaa gtcttccgga agtttgctag
    33961 aacgctgaat ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca
    34021 attgataaga aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc
    34081 cccattgtcc ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt
    34141 ttttaggcga catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa
    34201 agtcttcctg attcgggtcg ccgatccctc tgatgctaaa gaatgatttt ttaggaacct
    34261 ctagtatttg cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt
    34321 ccatttttct tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt
    34381 gacttggcta atcgtgttga cactattttt cagtttgtgc tagaataagt gaagtaaata
    34441 aatcgtacag ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata
    34501 gaaatcactc tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac
    34561 agctgctttt agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga
    34621 tcgagacggc catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg
    34681 cacaagtcca atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt
    34741 ctacttattt gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt
    34801 tcttggcagg tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc
    34861 gattgctcca aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga
    34921 catttgcccc cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca
    34981 ttgctccagc aattggcccc acttatggag gcattatgac ttcacatttt acatggaatc
    35041 atatcttcct gtttcttgtg cccgttttgt ttctttcttt gattatcggc tggtttgcta
    35101 ttcccgagat ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt
    35161 gtcttttgtt tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga
    35221 ttgctttaat catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg
    35281 agcatccact gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg
    35341 gcttcttagt ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc
    35401 agatcgttca aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg
    35461 ttggtgcttt gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc
    35521 caatttttgc tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag
    35581 gacacgcttc agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat
    35641 tatcttacag caatatgatg accaccggaa tgaatgcact gaatgaagaa ctacaaggag
    35701 acgggaacgc aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag
    35761 tagcggtcat tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta
    35821 cattaggatc aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc
    35881 gattcgccta ttatctgttc tttgctaaaa aagcgtagat tccatatacg gcaggtgttg
    35941 catctgccgt ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa
    36001 aattgccatt atcggtggcg ggatcattgg tatgacccta gccagttact tagatacatc
    36061 aaaattcaat gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg
    36121 gattatctcc ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga
    36181 cggcgctgct tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc
    36241 acaatcaggg acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc
    36301 cgagaaacga aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca
    36361 gacatctcaa gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg
    36421 tcgtctggat ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat
    36481 ggagcagatc caggcaaaag tcactttgca taagaagagc gattcttggc tcatcaaggg
    36541 accgtttgga gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc
    36601 tttattacaa gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc
    36661 atttcagaca tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc
    36721 tgatttcatt cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca
    36781 aggctgggat ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca
    36841 gttcttgact gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg
    36901 tgcttatacg tcagattttt ccccattttt cggcgtactg ccagatgatc caagcttact
    36961 ggctgctagc ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt
    37021 agccaacttc ttaaatactg gtcattggcc tacggaagat tatcaaaagg acatctccac
    37081 ctatattacg aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat
    37141 attgcgcatg gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt
    37201 ctccaaactt tcttttgctt catctggcaa gagatctttt cctatctgag cttcaatgaa
    37261 tcgtaaacga atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga
    37321 aacaggaaaa tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa
    37381 ttctgccgtt tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt
    37441 tttatcatcg aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa
    37501 ctctcgaaaa gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg
    37561 ttcaatgact agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt
    37621 tgttgctaat gttgcaccaa aagacgctgc taaaactggg tcattgatct tgataattgg
    37681 cggaagcgaa tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc
    37741 gactcggtcg ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc
    37801 tcctgttact aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt
    37861 aaggaaagaa gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga
    37921 aggttccaag gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa
    37981 gatcgaaaca gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc
    38041 ggacaaccca aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc
    38101 accgtggcag gcaataaagc cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc
    38161 tttttttaat tctccaaagt aacgtaatcc tagcacagcc gctgtattat ggagagcatc
    38221 aaacaaaatg agtccatttg gaaatgtttc atgtgtccat tcgggatcac aaaagatata
    38281 aagatctccc tcatcaaagg aaacagactt ttgataagtc tctaagatct tcgaattcgc
    38341 tggctgaaaa ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa
    38401 atgactttgc acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg
    38461 gcgcttgcga ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt
    38521 gtctaagtcc tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt
    38581 gaatactagt gtcctaacat cagctggtaa atctagttct tcagcatgtg agattgttaa
    38641 atctgtgatg atcgttcctg gactcccttt tgctagttga taagctgtct tcctatcaaa
    38701 aacttcatga acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa
    38761 cagtggattt ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc
    38821 tcctcattcc ctaaacaaaa aaacgttttc tttattatac attattcgag tttttatttg
    38881 tactgctgcg ctgtgggaag caaacgagtt atttttcaca caaaaaaacc gagaacttga
    38941 ataaacaagt ttctcggttt catcattttc cttttatcgg cttagtacca gccgtttgct
    39001 aaccagaatg ttttagcagc atcccatgaa ccataacgag atgttacata ttgttctgct
    39061 acacgttctt gattggcagc tgaatagtcg ccgtttaagt atgaagcatc taattgataa
    39121 cggccgatgt aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc
    39181 cattcttttg cagagcttga agttggcgct gttgcagcag ctggggcagc aggagctact
    39241 tcaactgttt caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc agttgtagat
    39301 gctgtatttt ctgttgtttt gttttctgtt gttgctgaag aatcatttga agttgggatt
    39361 gttaattgtt caccaacata aatgcggttg atgtttgcga tcgaattttt ttcagcaatt
    39421 gcatcgatca ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa tgaatcacct
    39481 gattttactg tataaacttc atcagcgtga gcatttgtag ttcctacaaa cattgcagta
    39541 gcaccagcag caaaagttgt acctaaaaca attttcttaa atgaattcat attaaactcc
    39601 tttttttctc cgttttttat tctttcgacg ctattaactt tatcatttag aaatatttca
    39661 taggtaaaag aaacatgaca tttaatgaca aaataacgaa ttatttaata tttgtaacat
    39721 ttaaacaaat atttttttga tacaaaacca taaaagcctt gttgtataag caaagtaatc
    39781 gtttttctat gt
//
LOCUS       DAYYAO010000018        37733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000018 DAYYAO010000000
VERSION     DAYYAO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.019"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..966)
                     /locus_tag="ACHARI_000850"
     CDS             complement(22..966)
                     /locus_tag="ACHARI_000850"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8441915.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1262..2206)
                     /locus_tag="ACHARI_000851"
     CDS             complement(1262..2206)
                     /locus_tag="ACHARI_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8441916.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2427..3641
                     /locus_tag="ACHARI_000852"
     CDS             2427..3641
                     /locus_tag="ACHARI_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8441917.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3663..5678
                     /locus_tag="ACHARI_000853"
     CDS             3663..5678
                     /locus_tag="ACHARI_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8441918.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5761..7134)
                     /locus_tag="ACHARI_000854"
     CDS             complement(5761..7134)
                     /locus_tag="ACHARI_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8441919.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7160..7444)
                     /locus_tag="ACHARI_000855"
     CDS             complement(7160..7444)
                     /locus_tag="ACHARI_000855"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8441920.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7446..9461)
                     /locus_tag="ACHARI_000856"
     CDS             complement(7446..9461)
                     /locus_tag="ACHARI_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8441921.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9640..9945)
                     /locus_tag="ACHARI_000857"
     CDS             complement(9640..9945)
                     /locus_tag="ACHARI_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8441922.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10034..11065)
                     /locus_tag="ACHARI_000858"
     CDS             complement(10034..11065)
                     /locus_tag="ACHARI_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8441923.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11272..12147
                     /locus_tag="ACHARI_000859"
     CDS             11272..12147
                     /locus_tag="ACHARI_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8441924.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12299..13141)
                     /locus_tag="ACHARI_000860"
     CDS             complement(12299..13141)
                     /locus_tag="ACHARI_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8441925.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13233..15110)
                     /locus_tag="ACHARI_000861"
     CDS             complement(13233..15110)
                     /locus_tag="ACHARI_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8441926.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15107..16528)
                     /locus_tag="ACHARI_000862"
     CDS             complement(15107..16528)
                     /locus_tag="ACHARI_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8441927.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16531..19920)
                     /locus_tag="ACHARI_000863"
     CDS             complement(16531..19920)
                     /locus_tag="ACHARI_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8441928.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20177..21568
                     /locus_tag="ACHARI_000864"
     CDS             20177..21568
                     /locus_tag="ACHARI_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441929.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21641..22288)
                     /locus_tag="ACHARI_000865"
     CDS             complement(21641..22288)
                     /locus_tag="ACHARI_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8441930.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22296..22982)
                     /locus_tag="ACHARI_000866"
     CDS             complement(22296..22982)
                     /locus_tag="ACHARI_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8441931.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22988..24061)
                     /locus_tag="ACHARI_000867"
     CDS             complement(22988..24061)
                     /locus_tag="ACHARI_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8441932.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24283..24957
                     /locus_tag="ACHARI_000868"
     CDS             24283..24957
                     /locus_tag="ACHARI_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8441933.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24962..26302
                     /locus_tag="ACHARI_000869"
     CDS             24962..26302
                     /locus_tag="ACHARI_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8441934.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26451..27437)
                     /locus_tag="ACHARI_000870"
     CDS             complement(26451..27437)
                     /locus_tag="ACHARI_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8441935.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28161..29192
                     /locus_tag="ACHARI_000871"
     CDS             28161..29192
                     /locus_tag="ACHARI_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8441936.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29301..30833
                     /locus_tag="ACHARI_000872"
     CDS             29301..30833
                     /locus_tag="ACHARI_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8441937.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            30972..31784
                     /locus_tag="ACHARI_000873"
     CDS             30972..31784
                     /locus_tag="ACHARI_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8441938.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            31774..32259
                     /locus_tag="ACHARI_000874"
     CDS             31774..32259
                     /locus_tag="ACHARI_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8441939.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32282..32827
                     /locus_tag="ACHARI_000875"
     CDS             32282..32827
                     /locus_tag="ACHARI_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8441940.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(32876..34543)
                     /locus_tag="ACHARI_000876"
     CDS             complement(32876..34543)
                     /locus_tag="ACHARI_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8441941.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNKKNFLVKVH"
     gene            complement(34672..36900)
                     /locus_tag="ACHARI_000877"
     CDS             complement(34672..36900)
                     /locus_tag="ACHARI_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8441942.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(36933..37412)
                     /locus_tag="ACHARI_000878"
     CDS             complement(36933..37412)
                     /locus_tag="ACHARI_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8441943.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        10991 a         7159 c         6875 g        12708 t
ORIGIN      
        1 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
       61 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
      121 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
      181 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
      241 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
      301 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
      361 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
      421 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
      481 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
      541 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
      601 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
      661 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
      721 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
      781 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
      841 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
      901 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
      961 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
     1021 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
     1081 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
     1141 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
     1201 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
     1261 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
     1321 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
     1381 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
     1441 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
     1501 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
     1561 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
     1621 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
     1681 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
     1741 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
     1801 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
     1861 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
     1921 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
     1981 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
     2041 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
     2101 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
     2161 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
     2221 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
     2281 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
     2341 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
     2401 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
     2461 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
     2521 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
     2581 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
     2641 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
     2701 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
     2761 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
     2821 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
     2881 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
     2941 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
     3001 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
     3061 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
     3121 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
     3181 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
     3241 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
     3301 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
     3361 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
     3421 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
     3481 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
     3541 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
     3601 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
     3661 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
     3721 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
     3781 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
     3841 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
     3901 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
     3961 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
     4021 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
     4081 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
     4141 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
     4201 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
     4261 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
     4321 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
     4381 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
     4441 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
     4501 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
     4561 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
     4621 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
     4681 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
     4741 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
     4801 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
     4861 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
     4921 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
     4981 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
     5041 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
     5101 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
     5161 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
     5221 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
     5281 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
     5341 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
     5401 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
     5461 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
     5521 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
     5581 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
     5641 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
     5701 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
     5761 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
     5821 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
     5881 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
     5941 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
     6001 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
     6061 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
     6121 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
     6181 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
     6241 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
     6301 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
     6361 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
     6421 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
     6481 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
     6541 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
     6601 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
     6661 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
     6721 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
     6781 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
     6841 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
     6901 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
     6961 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
     7021 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
     7081 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
     7141 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
     7201 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
     7261 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
     7321 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
     7381 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
     7441 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
     7501 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
     7561 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
     7621 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
     7681 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
     7741 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
     7801 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
     7861 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
     7921 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
     7981 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
     8041 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
     8101 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
     8161 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
     8221 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
     8281 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
     8341 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
     8401 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
     8461 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
     8521 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
     8581 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
     8641 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
     8701 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
     8761 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
     8821 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
     8881 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
     8941 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
     9001 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
     9061 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
     9121 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
     9181 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
     9241 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
     9301 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
     9361 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
     9421 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
     9481 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
     9541 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
     9601 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
     9661 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
     9721 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
     9781 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
     9841 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
     9901 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
     9961 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    10021 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    10081 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    10141 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    10201 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    10261 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    10321 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    10381 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    10441 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    10501 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    10561 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    10621 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    10681 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    10741 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    10801 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    10861 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    10921 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    10981 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    11041 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    11101 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    11161 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    11221 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    11281 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    11341 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    11401 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    11461 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    11521 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    11581 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    11641 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    11701 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    11761 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    11821 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    11881 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    11941 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    12001 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    12061 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    12121 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    12181 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    12241 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    12301 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    12361 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    12421 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    12481 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    12541 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    12601 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    12661 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    12721 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    12781 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    12841 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    12901 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    12961 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    13021 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    13081 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    13141 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    13201 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    13261 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    13321 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    13381 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    13441 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    13501 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    13561 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    13621 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    13681 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    13741 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    13801 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    13861 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    13921 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    13981 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    14041 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    14101 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    14161 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    14221 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    14281 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    14341 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    14401 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    14461 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    14521 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    14581 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    14641 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    14701 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    14761 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    14821 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    14881 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    14941 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    15001 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    15061 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    15121 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    15181 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    15241 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    15301 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    15361 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    15421 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    15481 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    15541 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    15601 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    15661 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    15721 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    15781 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    15841 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    15901 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    15961 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    16021 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    16081 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    16141 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    16201 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    16261 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    16321 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    16381 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    16441 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    16501 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    16561 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    16621 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    16681 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    16741 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    16801 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    16861 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    16921 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    16981 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    17041 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    17101 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    17161 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    17221 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    17281 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    17341 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    17401 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    17461 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    17521 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    17581 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    17641 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    17701 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    17761 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    17821 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    17881 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    17941 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    18001 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    18061 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    18121 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    18181 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    18241 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    18301 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    18361 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    18421 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    18481 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    18541 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    18601 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    18661 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    18721 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    18781 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    18841 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    18901 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    18961 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    19021 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    19081 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    19141 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    19201 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    19261 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    19321 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    19381 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    19441 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    19501 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    19561 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    19621 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    19681 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    19741 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    19801 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    19861 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    19921 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    19981 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    20041 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    20101 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    20161 attaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    20221 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    20281 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    20341 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    20401 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    20461 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    20521 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    20581 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    20641 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    20701 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    20761 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    20821 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    20881 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    20941 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    21001 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    21061 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    21121 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    21181 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    21241 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    21301 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    21361 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    21421 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    21481 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    21541 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    21601 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    21661 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    21721 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    21781 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    21841 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    21901 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    21961 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    22021 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    22081 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    22141 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    22201 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    22261 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    22321 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    22381 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    22441 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    22501 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    22561 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    22621 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    22681 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    22741 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    22801 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    22861 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    22921 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    22981 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    23041 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    23101 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    23161 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    23221 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    23281 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    23341 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    23401 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    23461 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    23521 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    23581 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    23641 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    23701 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    23761 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    23821 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    23881 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    23941 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    24001 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    24061 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    24121 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    24181 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    24241 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    24301 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    24361 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    24421 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    24481 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    24541 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    24601 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    24661 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    24721 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    24781 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    24841 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    24901 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    24961 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    25021 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    25081 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    25141 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    25201 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    25261 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    25321 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    25381 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    25441 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    25501 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    25561 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    25621 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    25681 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    25741 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    25801 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    25861 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    25921 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    25981 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    26041 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    26101 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    26161 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    26221 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    26281 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    26341 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    26401 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
    26461 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    26521 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    26581 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    26641 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    26701 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    26761 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    26821 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    26881 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    26941 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    27001 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    27061 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    27121 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    27181 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    27241 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    27301 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    27361 cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt
    27421 aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt
    27481 acttataaat ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg
    27541 attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata
    27601 aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg
    27661 tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat
    27721 tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac
    27781 tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa
    27841 taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa
    27901 gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag
    27961 ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta
    28021 aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta
    28081 agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac
    28141 actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa
    28201 agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt
    28261 ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt
    28321 tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag
    28381 attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga
    28441 accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt
    28501 cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc
    28561 ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac
    28621 ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag
    28681 ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt
    28741 gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc
    28801 agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag
    28861 cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt
    28921 ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga
    28981 gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt
    29041 atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc
    29101 tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat
    29161 tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat
    29221 aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa
    29281 caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta
    29341 cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac
    29401 tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt
    29461 tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt
    29521 ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag
    29581 tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt
    29641 tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa
    29701 atggaacaaa atcaaaacag attcctattc tttatttaat ggaacaacct gctttgtctg
    29761 tttctcaatc atttttagac aaaaatgaaa tttcttatat gacccatact tctggtacaa
    29821 ctggtatccc taaacttatt tgtcattctg ctcattctat gggatggcgg acgaaatggc
    29881 aaaaaacagt tttcacaaaa atttcggaaa agaaattaat cgcttttcac atctctccag
    29941 tgcattccag attcaatatt ggtatctctt ctttgatgtc catgggattt cctatgatgc
    30001 ctttagcaaa cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac cggccaattg
    30061 cactagagac ccatccgaat aactttgttc agtggcgttt tactgctaag gagcatccag
    30121 aagctttttc gggtatccga tactatcact ccacttttga tgctataaac aaccagacaa
    30181 tgaagacatt tttgcgaacg tcgatggcac aggatgcggt atttcttcaa gtatatggcc
    30241 aaagtgaatg cggaccaatg attcttaagg ctcatacact agagagtttg aaaacaagtg
    30301 atgcaagaga tatgggcgtt ggactggaag acatgacaag cgctcggatt acagattcag
    30361 ttgggaatgt cctacctgaa aacacagatg gtcatataca attactgtct aaaggacggg
    30421 ctttgactta ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc gattggtggg
    30481 atagcggaga ttacggtttt atggataagc atggccacct atttttgaaa gatcggcaag
    30541 tggatttgat cgaaacgatc aatagtacat tagctattga agacttttta ttagattcgt
    30601 tagatttttt ggcagaagta gtgattgtaa gggataaaaa tcattctcca caaccgatta
    30661 ttgctttagc tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa gtccatgaac
    30721 ttcctcgctt gaacgtccct atcatccgtg attttgatgc aatcccgcga acagctacaa
    30781 tgaaagtgca acggcttcaa atcgagaaag aattaaaatc acaaaagtcc taactattta
    30841 aggaaagaaa gatccatttt ggtctttctt ttctttttgt ttttttaaat tggatgagat
    30901 aaaaatatgc caattggctg tttttctttt ttgaaaattt cgttatactc ttattcatgg
    30961 aggaaaatgt tatgaaaaga atcttgacaa ttggcggttc tgatccattt gctggtggag
    31021 gaatccaaac agatttaaaa acttttgaga attttggtct attcggacta agcgccttga
    31081 cttctatagg ggcactagat attaatgagc gatttatgct ggaatctata tctttggaat
    31141 tgcttaaacg acagttagtt tcgattgata aaatgacttc attagatggt atcaaaattg
    31201 ggttattgaa ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa acaaaacaaa
    31261 atatcccaat cattttagac cctgttttgg cattcaaaga aactgaagct gtgatcaatc
    31321 aattgtatat ttcgcagctc atcgaagaac tttttccact tgttacgatg attacgccta
    31381 atttggcaga agctgcttta ctttctggtc aagctcttcc ctcttcgtta acagaaatga
    31441 tccatctcgc taaagaactc actgcttcag gaacaaaaaa cattgtgatc aaaggcggca
    31501 caaggatggc tggagatgaa gctgtggatc ttctctattc aattgatcat tatgaggtat
    31561 tttctcataa aaaaatcgac actacaacag taaacggggc cggttgtgcc ttttcttctg
    31621 caataacagc gaatcttgtg ttaggggcag attttaccaa agcagtagca gaaagtaaaa
    31681 aatttgttgc ccattgtatt tctaatggcg tgctgatgaa cgatcatacc ggaagtgtct
    31741 ggtctggcgg tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt aacgatctat
    31801 gctatgctga ttgcgttgac agttgccctt tcattaacaa ttttgatccc tgtaccggct
    31861 acgaatggat ttgtgacatt atgcgaggcc gggatctata ccgctgcttt tctttatggt
    31921 ccaacgggtg gattagttgt tggtgcttcc agtggtctat tgatcgattt gatttcagga
    31981 tatccgcagt gggcagtttt ttcattagtg atccacgggt tacaaggata tgttgctggt
    32041 tctttcagca aatcttctac tcgtatgtgg ggattagggc ttttattagg aagcttagtt
    32101 atgatcatcg gctatggtat cgccggttgg attttatatg attggccggc tggagtggct
    32161 tctataccag gaaacatctt ccaaaatctg attggaatgg gacttgcctt tcctataact
    32221 gcaagtcttc aacgtattca agcacggaag caatattaat tgaaaaagaa ggttttagaa
    32281 aatgaagttg tcagaaaaag aattaaaaga ccaattgact gaaatagtta atgacatatt
    32341 ggctgaggca cacttgaaaa aaggtgatct ttttgtttta ggatgcagca caagcgaagt
    32401 cgttggagga catattggaa aaaattccag tgcggaagtt ggtcaatgga tcatccgtac
    32461 gttaaaagag ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt gtgaacattt
    32521 gaaccgagca ttagtagtag aacgaacagt ggcagaagca aaaaatttcg aaattgtctc
    32581 tgttgttcct gcactacatg ctggaggtgc ttgttctgtt gctgcttttg accagttcaa
    32641 tgatcctgtc gaagtggaac acgtagttgg acaagccggt atcgatatcg gtgacacatc
    32701 aatcggtatg catatcaagc atgtccaagt acctgtcaga cctcgtttga aaacccttgg
    32761 acaagctcac gtgacagccc ttcgctctcg tcctaaatat atcggtggtc ctagagcgaa
    32821 ttattgaaat gagtaattga cagagcaatc tgaaaaactt cctcgcaaag tccaactaat
    32881 gaactttaac gaggaagttt ttcttgtttg ttttatataa ccgagcttca taaggtgcta
    32941 gtatgccttt agcttcaaat tctccgctag aggaatttga caagagtaat gtccaggact
    33001 gatctgcttc ttttggaagt tcatagtcag caagtgattc tgttaagttt accataataa
    33061 gatattgttc atcttctaaa tatctttcat agacataaag ttgtggatgt tcatgaagat
    33121 attctttata agaaccgtat atcagacctt tattggtttg tctaatgcgg atcatgttct
    33181 tatagaattg caggaccgac tctggttcat attcttgttg ttctacattg atcgttttgg
    33241 tatttggatt agtgatcagc caaggttcat gtgtagaaaa accagcatat tgttccgaag
    33301 tccattgcat gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata tccaatgctt
    33361 cttctcttgt cttaccttcc gcaagcagcc ttttataaag tcttttcgaa tctactgcat
    33421 caacttgatc gatcgattca aagggcatat tcgtcattcc gatttcctgt ccttgataaa
    33481 taaatggcgt accttgcaaa aagaaataca tcatagcgat tgcttttgca gacatagccc
    33541 aaaaatcggg acgagtatct ccaaacactg aaacggcacg aggaacatca tggttttcca
    33601 tatataaagc gttccatccg ataccatcca atgaagtttg ccaagcgctt aatgcatgtt
    33661 tcaatttaag gacatctaat tgcccttctt tttcttgctg ccagatatgg atatgatcaa
    33721 attcgaatat catatcgaaa taaccgtctt tccctaccca ttgtggtcct tcttctgcag
    33781 tgacaccact tgcttctccg acagtcatga tatcgtactc ttcaaaaaca tgtttcaaat
    33841 cggtcaaatg ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg ttctctgtgg
    33901 cttttactga atattcatct ttttttacat gagaaatcgc atctaaacga aatccatcta
    33961 tccctttatc caaccaccat cggatcatat tgaatagttc ttctttcaat ttctcgcttt
    34021 cccaatttaa gtctggttgt tcttttgcaa aaacatgcag ataatactgt ccagtttcat
    34081 gagaatactc ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt tctttgccat
    34141 ctaccccgtc gacccaaatg taataatcgc gatacgggct ttctttagat tttttcgatt
    34201 cttcaaacca ttgatgctga tccgaactat ggttgatgac aagatccata atcactttga
    34261 tccctaattc atgtgcttct tttagcaata aatcaaaatc ttccatcgtt ccgaatctct
    34321 cttctatatc ttgatagtca cttacgtcat aaccattatc gacaaatgga gattgataga
    34381 tcggattgat ccagataaag ccgataccaa gatctttcaa ataattaagt ttttcacgga
    34441 tcccattgat gtctccttga ccatcgccat tgctatcttt gaaactagca ggatagattt
    34501 gatagcctac tgcttctttc caccattttt gttcttgtat catatcgttt cactccttta
    34561 acggtccctc ttgattataa ccgattctat tttagaagta tccttgttac cggtaactag
    34621 aaacgaaaag ctcaacgaat ctgctaagtg cgttcgttga gctttatttt atcatagata
    34681 gcgggcaatc agttgaatca tttgcttggg aaattcattc aagtcagtaa tatctacaaa
    34741 acgactttca ccgtagatcg acttgatttc ttctttgtct tggccaatag ctgcggagac
    34801 aaataggatt ccttgacgtt catattcttt caaaacagac tggatatctt cttttgcttt
    34861 tttgcctgta taatccggca acgcttttgg ctgaccgtca ctaatattaa gcaacagttt
    34921 ggttgttgcg gattgtctgt ttaatttttc tgctgctaaa cgaagtgcag caccatcacg
    34981 attgttttgc cttggtttca tagtgactaa tttttcattt aaccattgaa atccgtcttc
    35041 aaattcttta taagaaaaca aagaagtctt ttctcttgac gaacggtctg ctgtatctcc
    35101 gtaaatcaac aatgggatat cgactttctt agaaaattcg gcaatggcaa tcgctgcttt
    35161 ttttgccgct tcgatccgat catcccgtat cattgaccca gattcatcga tccgtacagc
    35221 taccgctaaa gaaggttgct catgtggcgg gttctttttg tcaaaatttc tcaaatcacc
    35281 atatgctact ttgcttgcat tgaaccgctg accttcgtat tttccttttt ggtacgtctc
    35341 tgtttgttca ttatccagta actctaaaat ttgccggcta agtgattcta ctattggcat
    35401 tacttgcatg tgtagctcac gtgcttccgg cgtttttcct tggaaagctg gtcggtgaac
    35461 gatgagacct tctttttcat gaatcgtttc cttcatgatt tgtcgcgctt ctttattcag
    35521 ttgcttcttg atttccttat catgtgcttc catgtcagaa gcagacattt cttgagaatc
    35581 atctcctgtt tgttgttctt tttgattttt tccttcgata tcgctactgc gttctttttg
    35641 catttgagga ggaatctcgt ttttttctat tgtttcttct tgtttctctt ctccctcatt
    35701 tggttcttca gcagacaacg atttcttttc atctaagcgc agaatttctt cagcattttc
    35761 agtgatgttt cgttcatttg cttcttcggc gctaatggtt ctttcttttg tatgctcaga
    35821 ttgaggatga ccccattttt cttttctcaa tatgtcctga agttccagta gcaatccact
    35881 gtcagacaaa gcttgttcaa gaattgccaa ttcatctgaa tcagtggata atttatacaa
    35941 aacttttgga taaatcgact ggagtaaatc ttgtccttgc ttataggtat ttgcccaata
    36001 aaaataagaa cgcattcctg ctacaccttt gatggcgtta gcttttgcag tttcatccaa
    36061 caaacggata atctctgcca tctttaagag aagttcttgt ttttggatca tagttttcgc
    36121 catcgcacgt tctgccatga cttctaaggc aggaagatcc attttttccg catgttgcat
    36181 ccgatctctt aaagattcat ttaatgggcg atttccttgg taatttcgat ttgtagtgat
    36241 cacaatgacg caatctggat ggcgacgaat gattcctgtc ggtaaattca acatcccatt
    36301 tggttcgagc gctgagttaa gtgccacaag aacagaggca tctcggatga cagtcggctc
    36361 ttgaatctct aataaatagc ctttttcaat cgctcgaacg atttcagaag ggtaatagct
    36421 gtaatggatt gcatctggat aatcagcttc gatcctttga actagctctg ctaatttttc
    36481 tttagcagaa aaaggatcaa tgccgtaatg gttctggatc agtgtcagta cagcttcaag
    36541 tgtatctgtt tggtaaatag cttctagtaa ttcttggtct tgttccttat tagaatctac
    36601 tacaggtaat aaggctccga aaacatctga tttgtccata tctgcaaaac aggtcacttt
    36661 tgtataggga agttgcagat cagcagatag tgctttggct aattgtgtct tccccgaccc
    36721 ggcatctcct tccaaaagta cgttactgat tttcatttca ggatcttgcc aattcccttt
    36781 gatttcggag gcgatccgcc attcttctgc acttgtccga tggcttttcg gtttcttcca
    36841 gatcatttgc ttttctagcc gtgaaaaggt tcggttttct gttagtgaga aattctccat
    36901 gcttctcttc cccttttgat catctctgtc tattaatatc ctagctcatc taaaagacgt
    36961 tttaaaatac ggaatcgttc cccaatcagt tctccgtctt tttgtagtgt atcgtaatac
    37021 aaaatgatgt cttcatcgat ctttggattt ttggtatcta cttctaccgt cattcggttc
    37081 ccttgtaatc caatgatgtc tacgactgga ttttctggat cataaaattt ttcataacga
    37141 taagcatctt ggaagtataa acttccttgc gcagctaaga tcgctaaatc taaaatacgt
    37201 gtgattggta attcttccga ctgtcgtgac catttttctc cagtatgtcg ccataccttg
    37261 cctgagatat cgacttttcc acgatcattc cactgagcaa ggcccagtga aagccctttt
    37321 gcatccatat ttaatgcatt tcttccatca actttatcat agtcttctac aacgattact
    37381 ggtttatgtt ttaaatttgt tggtatttcc attattttaa ctcctcatcc ttttattttt
    37441 agtaaataag taaattagta gtttactcat ttactaaact atatgcgaca ttatagagga
    37501 ttgaagtgag gattgcaaag aaaaagagct tcattgatag aaataaaaac accatctcgt
    37561 tcttttacac gctcattagt aacatgtaga aagtccaaga tgatgttgga atattttggc
    37621 tctatacttt ttgatgttgg tgaacttcac caacatcttt tttgtattct taattgtcaa
    37681 attaagttag acagatcatc aatacttacg taactcaaaa atacttccaa agg
//
LOCUS       DAYYAO010000019        37569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000019 DAYYAO010000000
VERSION     DAYYAO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.020"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..520
                     /gene="mraZ"
                     /locus_tag="ACHARI_000879"
     CDS             89..520
                     /gene="mraZ"
                     /locus_tag="ACHARI_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8441944.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            541..1500
                     /gene="rsmH"
                     /locus_tag="ACHARI_000880"
     CDS             541..1500
                     /gene="rsmH"
                     /locus_tag="ACHARI_000880"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8441945.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1505..1906
                     /gene="ftsL"
                     /locus_tag="ACHARI_000881"
     CDS             1505..1906
                     /gene="ftsL"
                     /locus_tag="ACHARI_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8441946.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1907..4099
                     /locus_tag="ACHARI_000882"
     CDS             1907..4099
                     /locus_tag="ACHARI_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8441947.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4125..5087
                     /gene="mraY"
                     /locus_tag="ACHARI_000883"
     CDS             4125..5087
                     /gene="mraY"
                     /locus_tag="ACHARI_000883"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8441948.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5101..6462
                     /gene="murD"
                     /locus_tag="ACHARI_000884"
     CDS             5101..6462
                     /gene="murD"
                     /locus_tag="ACHARI_000884"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8441949.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6488..7576
                     /gene="murG"
                     /locus_tag="ACHARI_000885"
     CDS             6488..7576
                     /gene="murG"
                     /locus_tag="ACHARI_000885"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8441950.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7591..8811
                     /locus_tag="ACHARI_000886"
     CDS             7591..8811
                     /locus_tag="ACHARI_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8441951.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            8967..10292
                     /gene="ftsA"
                     /locus_tag="ACHARI_000887"
     CDS             8967..10292
                     /gene="ftsA"
                     /locus_tag="ACHARI_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8441952.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10315..11556
                     /gene="ftsZ"
                     /locus_tag="ACHARI_000888"
     CDS             10315..11556
                     /gene="ftsZ"
                     /locus_tag="ACHARI_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8441953.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11573..12250
                     /locus_tag="ACHARI_000889"
     CDS             11573..12250
                     /locus_tag="ACHARI_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8441954.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12264..12863
                     /locus_tag="ACHARI_000890"
     CDS             12264..12863
                     /locus_tag="ACHARI_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8441955.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12944..13165
                     /locus_tag="ACHARI_000891"
     CDS             12944..13165
                     /locus_tag="ACHARI_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8441956.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13214..13996
                     /locus_tag="ACHARI_000892"
     CDS             13214..13996
                     /locus_tag="ACHARI_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8441957.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14017..14724
                     /locus_tag="ACHARI_000893"
     CDS             14017..14724
                     /locus_tag="ACHARI_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8441958.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            14986..17784
                     /gene="ileS"
                     /locus_tag="ACHARI_000894"
     CDS             14986..17784
                     /gene="ileS"
                     /locus_tag="ACHARI_000894"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8441959.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17833..19359)
                     /gene="zwf"
                     /locus_tag="ACHARI_000895"
     CDS             complement(17833..19359)
                     /gene="zwf"
                     /locus_tag="ACHARI_000895"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8441960.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19374..20021)
                     /locus_tag="ACHARI_000896"
     CDS             complement(19374..20021)
                     /locus_tag="ACHARI_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8441961.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20205..20534)
                     /locus_tag="ACHARI_000897"
     CDS             complement(20205..20534)
                     /locus_tag="ACHARI_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8441962.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            20711..21439
                     /gene="sfsA"
                     /locus_tag="ACHARI_000898"
     CDS             20711..21439
                     /gene="sfsA"
                     /locus_tag="ACHARI_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8441963.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21455..22468
                     /locus_tag="ACHARI_000899"
     CDS             21455..22468
                     /locus_tag="ACHARI_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8441964.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22468..23745
                     /locus_tag="ACHARI_000900"
     CDS             22468..23745
                     /locus_tag="ACHARI_000900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8441965.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            23808..26510
                     /locus_tag="ACHARI_000901"
     CDS             23808..26510
                     /locus_tag="ACHARI_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8441966.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            26662..26979
                     /locus_tag="ACHARI_000902"
     CDS             26662..26979
                     /locus_tag="ACHARI_000902"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8441967.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27009..27329
                     /locus_tag="ACHARI_000903"
     CDS             27009..27329
                     /locus_tag="ACHARI_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8441968.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27437..28897
                     /locus_tag="ACHARI_000904"
     CDS             27437..28897
                     /locus_tag="ACHARI_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8441969.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            28965..29186
                     /locus_tag="ACHARI_000905"
     CDS             28965..29186
                     /locus_tag="ACHARI_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441970.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29217..29399
                     /locus_tag="ACHARI_000906"
     CDS             29217..29399
                     /locus_tag="ACHARI_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8441971.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29399..29812
                     /locus_tag="ACHARI_000907"
     CDS             29399..29812
                     /locus_tag="ACHARI_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8441972.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            29935..31116
                     /locus_tag="ACHARI_000908"
     CDS             29935..31116
                     /locus_tag="ACHARI_000908"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8441973.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31187..31351)
                     /locus_tag="ACHARI_000909"
     CDS             complement(31187..31351)
                     /locus_tag="ACHARI_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441974.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            31505..31578
                     /locus_tag="ACHARI_000910"
     tRNA            31505..31578
                     /locus_tag="ACHARI_000910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31539..31541,aa:Arg,seq:cct)
     gene            complement(31647..32786)
                     /locus_tag="ACHARI_000911"
     CDS             complement(31647..32786)
                     /locus_tag="ACHARI_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8441975.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            32958..33092
                     /locus_tag="ACHARI_000912"
     CDS             32958..33092
                     /locus_tag="ACHARI_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441976.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(33085..33720)
                     /locus_tag="ACHARI_000913"
     CDS             complement(33085..33720)
                     /locus_tag="ACHARI_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8441977.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(33833..34468)
                     /locus_tag="ACHARI_000914"
     CDS             complement(33833..34468)
                     /locus_tag="ACHARI_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8441978.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(34502..34963)
                     /locus_tag="ACHARI_000915"
     CDS             complement(34502..34963)
                     /locus_tag="ACHARI_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8441979.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(35093..35524)
                     /locus_tag="ACHARI_000916"
     CDS             complement(35093..35524)
                     /locus_tag="ACHARI_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8441980.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(35542..35862)
                     /locus_tag="ACHARI_000917"
     CDS             complement(35542..35862)
                     /locus_tag="ACHARI_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8441981.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            36161..36937
                     /locus_tag="ACHARI_000918"
     CDS             36161..36937
                     /locus_tag="ACHARI_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8441982.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            36952..37155
                     /locus_tag="ACHARI_000919"
     CDS             36952..37155
                     /locus_tag="ACHARI_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8441983.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            37171..37509
                     /locus_tag="ACHARI_000920"
     CDS             37171..37509
                     /locus_tag="ACHARI_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8441984.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        12522 a         6405 c         7682 g        10960 t
ORIGIN      
        1 gttgttcccg gatttcccag aaaaggtggt agaaagtggg ggattgtggt agactaattt
       61 tagttagtgg aggaatgggg ggctttcaat gttgatgggt gaattccaac ataatattga
      121 tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt
      181 tctgacacgc ggattggatg gctgtctttt cggctatccg atgagcgaat gggaaaatct
      241 ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt
      301 cttctattcg gctgccactg aatgcgaact tgataaacaa ggacgaatca atatacccag
      361 cacgttaaga aattatgcag ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg
      421 aattgaaatt tgggatgaga cacgatggca agatttttct gcagcagcag aagagaactt
      481 tgacgaaatt gctgaaagta tgattgattt cggactataa gaataggaag aggctgacta
      541 atgactgaaa catttcaaca ctatacagtc atgttgaaag aaacggtaga tggattgaac
      601 atcaaaccgg atggcgttta tgtagactgt acactcggcg gtgccggaca tagtgaatat
      661 ttattaagcc aactaagttc taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca
      721 atcgatcacg ccaagctgcg tttagcttct tatgtcgaac aaggtatggt cacatttatc
      781 aaagcgaact tcagagaatt agaagaacgc ttgagtgagc atgtagataa ggtagacggc
      841 attttgtatg atctaggggt ttcctctcct cagctagatg aagcagaacg tggatttagt
      901 tatcatcaag atgcaccgtt ggatatgcgc atggatcagt cagctccttt atccgcttat
      961 cacgtaatta atgaatattc ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag
     1021 gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc
     1081 caaacaactg gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga
     1141 aaaggcgggc atcctgcaaa aagaattttt caagctgtgc ggattgcagt aaatgacgaa
     1201 ctcggtgccg aagaggcctc tttagaacag gcaatccggt tattgaaagt caatggaaga
     1261 atcagcgtaa tcacattcca ttcattggaa gacagaattg tcaaaagtat gtttaaagaa
     1321 tatagtacgg tacaagatct accaccggga cttcctgtca tacctgacga attccaacca
     1381 gaactgaaag tcattactcg taaaccaatc cttccttcag aggaagagct aacagaaaac
     1441 aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa agaggagtag
     1501 aatgatggca gaattaaaga aaatgcaaga ttacgaatat gatttacctg aattggtgga
     1561 tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc
     1621 accggcaaaa cgattaaagc agatttctgc tttggaaaaa attgctgtgg caagcgtggc
     1681 gattgctatt gttgctttat gtgttttaac agttatgctg cgcacaaata tcagcggagc
     1741 tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag
     1801 tttagaacaa gaaaaaaacg aactttctcg tactgaacgg attaaacaaa tcgcagaaga
     1861 aaaaggattg tctattaatg acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa
     1921 taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg atgaacaatc gcaaaaaagt
     1981 aggtatcatt ttatttgcta cgagtattgg attgttcttt ttatttgccg tccgtttctc
     2041 gtatatcgtt attggaggtc atgttgctgg tacctcatta gctgaaaaaa caaaacagtt
     2101 gtaccaagga agtgaagtag taaaagcaaa aagaggaacg atttatgatc gtaacggtgt
     2161 ggcgctcgca gaagatgcct cctcttattc aattaaagcg atactctcaa aaacatatac
     2221 ctctggggat aaaaaactgt atgttgaaga aaagaatttt gataaaatag cagaaatcct
     2281 gcataagaat ctttctatag acaaaaaaga tgcactgaat attttagagg acggcgcaaa
     2341 aaaagagctg tatcaagttg aatttggtag ctatgggaaa aatatctccc aagaaaccaa
     2401 gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca
     2461 tcaggctcgg atgtatccaa atggcgtctt ttcttctcat tttattggat acgcagttcc
     2521 agacaaagat gagaatggct tagttggtaa actaggtttg gagtctgctt acaatgatat
     2581 cttgagcggt aaagatggta agattattta tcaaaaagat aattttcaaa atcctctgcc
     2641 aggtacagtg gcagaagaag aaaaagctgt cgacggacaa gatatctaca cgactttaga
     2701 cagcagacta caaagttatt tagaaacact gatggatcaa gtaaatgaag aatatcagcc
     2761 agaagaattg acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca
     2821 acgacctact ttcaatcctg aaacaatgga aggattgact ggaaaagatg ctatctggcg
     2881 taatttcttg gtacaagata gttatgaacc aggatcaacc atgaaagttt tcactactgc
     2941 agctgctatc gaagaaggag aattcaacga gaatgaaacc ttccaatctg gaaaaatcca
     3001 agtagaagat gctacgatca atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg
     3061 tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc gattaggcgg
     3121 acgatggtat aactatttac aaaaactggg atttggacaa agtactcatt ctggattaga
     3181 tgatgaagta aacggtgctt tacctacatt aaatattgtc gaccgagcaa tgagtgctta
     3241 tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa gctttcactt ctattgcgaa
     3301 taacggtaca atgattcaac ctcgatatat cagcaaagtg gtggatcccc aaactggaga
     3361 agagcgaaca actcaaacag aagttctagg gcaacctttt tcaaaagaaa caacagaaaa
     3421 agtccgtgaa tatatgagag atgttgtcga aagcgaaaac tacggtagtg cttatggtgt
     3481 atatagtgta cctggataca atgtatcggc aaaaaacgga acagcccaaa tcgcttctga
     3541 tacaggggga tatcaaacag gagatacagc ctacctgtat tcgattgtgg agatggttcc
     3601 ttctgaagat ccagactatg tcttgtatct aacaatgaaa caccctaaga catatgatcg
     3661 aatggcattg gctaaaatcg caaatccatt gatgaaacgg gcaatggatt ttaaagaaac
     3721 tgaagaagat tcagatacag aaacaaaaac agaaaaagta tccgtagccg attatcgtaa
     3781 tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat
     3841 cggtaatggt aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt tgatctctgg
     3901 agaaaaactg atcctatata ctggtggtga taagctgatg cctgacgtaa caggatggtc
     3961 aaaagcagat atcatgaagc tggggaaaat cctcggtatt gaagttagtt ttgatggaga
     4021 tggttattgt gtcaaacaag aacttgctcc atatgaaaaa atcactgaag acaaacttag
     4081 ttttacttta gaagaataag aagagaaata ggagagaaaa gagcatggaa tggacacaag
     4141 cattgattcc cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg ctatttattg
     4201 gttattttca aatgaaaaag caaggtcagg cgattcgtga ggagggacca aagtggcaca
     4261 atagcaaagc cggaactcct acaatgggcg gcttagtgtt tctgatcggc tctattttga
     4321 caggaatttg ggttggagct tggcaaaaac agctaactcc tactcttttc atcttgcttt
     4381 ttgtattagc gttatacgga gtaatcggct ttcttgatga ttttataaaa atattcaaga
     4441 aaagaaacat gggactgaac tcgaagcaga aattgattgg acagattcta gggggaatca
     4501 tcttttatct tgtttaccgt tctgaaggtt atccaggtgt attgaatttc ttcggactag
     4561 atctaccttt aggaatcatt tatggtattt tcgctctctt ctggcttgtt ggattttcaa
     4621 atgcagtaaa tctgacagat ggcatcgatg gattagttgc tggcttagga agtatctctt
     4681 ttgcagctta cggtctaatt gcttggcatc agcatcagta tgatgtatta atcatttgct
     4741 taagtgtttt aggaggttta ctgggtttct tcgtctacaa cagaaaacct gccaagattt
     4801 ttatgggaga tgtcggttca ttagcgttgg gcggactttt agcggcaatt tctataatgc
     4861 taaaccaaga atggacattg cttttgattg gtttgatcta tgttatggaa acagcaagtg
     4921 tgatgcttca agttacttcc tttaagctta caggaaaacg aatttttaaa atgtctccaa
     4981 ttcatcatca ctttgaaatg tgcggatggt cggagtggaa gattgataca gttttttggt
     5041 tgatcagcat tgtgacttcg ttgatcacat tatggatcgt ttggtaggag atgacgggaa
     5101 atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc
     5161 ggagtaagtg cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt taatgatgga
     5221 aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt ctttagggat aaaagtaata
     5281 acaggtagtc atccaatcga attgttggat gaagaatttt cattgatggt caaaaatcca
     5341 gggattccct acagtcatcc tttagtagca aaagcccaag aaatgggtat tccggtaatt
     5401 acggaagttg agctagctta cgaagtagcg gaatgcccaa ttattgggat tacaggaacg
     5461 aacggaaaaa caacgacaac aacgatgaca ggactactac tgaatgcagg agcagatcaa
     5521 ggaattgcac gtttagcggg taatattggg tatccagcaa gtggtgtagc gcaagaagca
     5581 aaatcagaag ataaaattgt gatggaattg tctagcttcc aactcatggg aatcacagat
     5641 ttccgccctc atatagcggt gattaccaat atctatgaag cacatattga ttatcatggt
     5701 acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac agaaaaagat
     5761 tatctgatcc ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca
     5821 cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac
     5881 tcaatatact ataaaaaaga aaaaataatg gaaatatctg aacttggcgt accaggtaag
     5941 cataatgtag aaaatgcact agctgctatt tctgtagcaa aattgtatgg tatatcaaat
     6001 gaagctatac gagaaacgtt gcattttttc catggcgtac cacatcgtac gcaatatgta
     6061 ggagaaatcc aaggcagaaa attttataat gattcaaaag caaccaatat tttagcgact
     6121 aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt tattggctgg cggattagac
     6181 agaggaaata catttgatga attaattcct tcattaaaag gaatcaaagc aatggtcgta
     6241 tttggccaaa caaaagaaaa gttgatggat gctggaaaaa aagcaggcat cgagacaata
     6301 gtaacagccg attctgtaga acaagcagtt cctttagcat tagaaaacag tacagatggt
     6361 gatgtagttt tattatcacc agcgaatgcc agctgggatc aatacccaaa ttttgaaact
     6421 agaggaaaca gattcatgga agctgtgaac cgattaaaat agaaaaagta gaagcgagcg
     6481 ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg ggacatattt atccagctct
     6541 tgcctttgtc aattatgtaa agactcaaga accgaatgct gaattcatgt atgtgggagc
     6601 aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga atgcctttcc ataccttgga
     6661 gattcaagga tttaaacgaa aaatttccat gcataacatc aagacggtcc gattgtttct
     6721 taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt caaccagata tcgttatcgg
     6781 tactggagga tatgtttccg gagcggtagt gtacgctgca gccaaaatgg gtattccaac
     6841 agttatccat gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa gccgatatgt
     6901 ggatcggatc gctctttcat ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt
     6961 tcttgtaggt aatcctcgag cacaagaagt agcagatact aaaaaatcag atattttgaa
     7021 aacatttgat ttggatccag aaaagaaaac agtcttgatt tttggcggta gtcaaggagc
     7081 acttaaaatc aatcaagcag tatcagtttt tttaagtgaa tatattcctt cttcttacca
     7141 agttttgtat gcttcaggag atagatatta tgaagagatc ctaaataaaa taaatcctga
     7201 aatacaaaat attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag
     7261 tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt
     7321 accggcagtg ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat
     7381 gagtcttgtt cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc
     7441 actaagtcag acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg
     7501 agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca
     7561 aataatcaaa aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg
     7621 tctcatccgt cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat
     7681 ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca
     7741 cccctttcag attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag
     7801 tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat
     7861 aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt
     7921 cgtcggttag ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat
     7981 gtttctcctt atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc
     8041 caagaaatca tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt
     8101 catcgatcta cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat
     8161 atacatttca aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct
     8221 ttgatcgcaa aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa
     8281 aaagtagcaa atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa
     8341 atcaaagaat taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct
     8401 gaaatcaagt acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac
     8461 ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa
     8521 gtagccaaag atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat
     8581 ccttacggta gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt
     8641 gaagaaaatg cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa
     8701 acacctggat cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt
     8761 caaatagaag ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac
     8821 tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt
     8881 gaatatatct attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga
     8941 atagtaagta ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata
     9001 ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta
     9061 ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata
     9121 aaacggtcca agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga
     9181 tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa
     9241 tgattgcagt taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt
     9301 cagctgccct tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag
     9361 attttactgt cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat
     9421 tagaaatgta cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat
     9481 gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg
     9541 aatcaattct ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg
     9601 gccaaacaac aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag
     9661 aaggtggcga atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg
     9721 cagaagcgtt gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag
     9781 aatttccagt agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt
     9841 cagaaattat ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc
     9901 aaatcgaagc attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc
     9961 ctggtgttgt ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa
    10021 atcacatggg gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag
    10081 caaacttgag tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag
    10141 ctcgtcaaat ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg
    10201 aagaaacaat ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa
    10261 tcaagggttt ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa
    10321 ttctcaattg ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt
    10381 ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt
    10441 acagccaaca cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta
    10501 ggccccaaat atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg
    10561 gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc
    10621 actgcaggaa tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg
    10681 aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg
    10741 aaacgtggcc gttttgctgc agaaggtatc gctcgtttga aagaaaatgt ggatacatta
    10801 ttaattatct caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa
    10861 gctttccgtg aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc
    10921 acagcgccag gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa
    10981 ggtacagcat tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg
    11041 aaaaaagcaa tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt
    11101 cttaacatca caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc
    11161 gtagcaaatg cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa
    11221 atgggcgatg aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa
    11281 cgcaagtcta gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta
    11341 ttagatatgg atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg
    11401 gatatcagac gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc
    11461 gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa
    11521 ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc
    11581 tgacaacttg cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga
    11641 tccgaaagaa gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga
    11701 attggttaga gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa
    11761 gaaacaagat ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg
    11821 cagaaaggta aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag
    11881 attagcagaa gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa
    11941 tgtttctggt gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga
    12001 atctttagct gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga
    12061 agcttctgat gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt
    12121 tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga
    12181 ctttcaaatt gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa
    12241 agacgaataa aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt
    12301 tcggattagc tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg
    12361 ctgtacagcc aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg
    12421 cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc
    12481 agccggctga aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga
    12541 aagaaagaac agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca
    12601 ctgtaatgga accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg
    12661 gagaagcaac cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg
    12721 attttctaac aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga
    12781 tttttctttg tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag
    12841 atgcgaattt gtttgatctg taagaggagg aactacgatt tatactattg cactattact
    12901 tcatttactt caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct
    12961 ctcttggttt ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga
    13021 accgtatatc agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat
    13081 tttaggggca atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg
    13141 gataactgtc catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata
    13201 ggaggggaaa attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt
    13261 tatcgattcg gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac
    13321 cgaatttctt gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga
    13381 tttgcgtttc atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt
    13441 ccccgattat tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta
    13501 tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg
    13561 gatcaaacgc tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat
    13621 tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt
    13681 acgcttggaa ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga
    13741 aagaggaaca gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc
    13801 cagacagcgg tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa
    13861 tacaagacct gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag
    13921 agtccagatc caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg
    13981 tgtattacgt aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc
    14041 caaaataaga cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc
    14101 ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa
    14161 gcagtcaaac attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac
    14221 caatcaatca tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa
    14281 tccgaagtga tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa
    14341 aaacgtgcac atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat
    14401 gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga
    14461 gtattccatc aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg
    14521 gaatgggatg aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc
    14581 aaagaagttt tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca
    14641 ggagtcatag aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg
    14701 cctggtgaaa atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg
    14761 atgagagtgg aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct
    14821 tttccgaaat tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca
    14881 ctagatagat gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac
    14941 atgctttttt gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa
    15001 accttgcaat taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc
    15061 gagtggcaaa aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa
    15121 ggaaaaccta gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta
    15181 ggacattcat tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc
    15241 tatcgtgcac catatgttcc aggatgggat acacatggat tgccgattga acaagtattg
    15301 gctaataaag gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt
    15361 gaatatgcac tttcccaagt agataagcaa cgagcagatt tcaaacgcct aggagttgca
    15421 ggagattggg aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc
    15481 gtatttggta aaatggctga aaaaggctat atttacaaag gattgaaacc aatttactgg
    15541 tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct
    15601 ccatctattt ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca
    15661 gcttttgtca tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta
    15721 aatccaaaat ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa
    15781 gatttgctag aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa
    15841 tttgctggac aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca
    15901 cttgtgattt taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct
    15961 ccaggacatg gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca
    16021 cctgtagact caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat
    16081 gacaaagcaa atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta
    16141 gatttcttta ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg
    16201 gcaactccac aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta
    16261 gaaaaagtag agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac
    16321 cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat
    16381 gctgaaaatg gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt
    16441 gctgaacatg gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga
    16501 tttacccacc caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc
    16561 tggtttgatt ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc
    16621 ccagcagata tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc
    16681 acgactagtg tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt
    16741 gctttagatg gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa
    16801 aaagtgatca aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat
    16861 gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa
    16921 atcagaaata cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat
    16981 actgtttcgt acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa
    17041 gtaatcaaag aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt
    17101 acagttatga acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat
    17161 gtagtttata ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat
    17221 caaacagctg tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa
    17281 atctggtcat tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat
    17341 gaagagtttg caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt
    17401 gataatgtct taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg
    17461 gaagcaaaac taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat
    17521 gcagatattg cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat
    17581 gaacaagtgc cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca
    17641 gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa
    17701 cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta
    17761 gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac
    17821 ccccttttgt tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca
    17881 gatccattcg aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc
    17941 agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac
    18001 gatatgccaa gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa
    18061 cgcatctaat agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt
    18121 tctaaattcc aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa
    18181 ggataaagaa aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt
    18241 cgtatcgtca catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa
    18301 tacgatgttg atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg
    18361 aacacctgac caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc
    18421 tgattgagca tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc
    18481 accttgagca tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg
    18541 gatagctttc aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg
    18601 cggttccatt gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag
    18661 tgcgccacta tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt
    18721 aatttggata ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa
    18781 acggatagct gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat
    18841 atcttgttct gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc
    18901 agaaccgaac ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg
    18961 tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac
    19021 acggttaccg tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg
    19081 ataatgcgtt gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt
    19141 tgcttcttca tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg
    19201 ctcatcgctc cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc
    19261 ttttctgtaa aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc
    19321 gccgaatatc gtaaacaata catttttttc attagacatg tggatacact cctttatatt
    19381 ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt
    19441 tggatagtga tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata
    19501 ttcaggtcaa tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata
    19561 cgcacttttg taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact
    19621 tcctcttttt taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt
    19681 tctaaatgat ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg
    19741 acttcattcc agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg
    19801 cgaatcagac tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg
    19861 gaatgttcta caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct
    19921 gagacatcta gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt
    19981 tctaaaatga ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg
    20041 gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat
    20101 aaaaaacaaa ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact
    20161 cattctttcg tttataaagg atatatatat acattgggag ggatctattt ttcagataac
    20221 aacttatatg aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata
    20281 agataaaaac catactgatc acgtacgact aaacgatatt catctatttc taccacagat
    20341 gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt
    20401 ttttgctggc tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt
    20461 tccgcttgtt ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca
    20521 agaaaataga tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag
    20581 acatattctt tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt
    20641 tcgactggtt tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag
    20701 gtgacataat ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg
    20761 gtttatagca cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac
    20821 aggtagagga aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg
    20881 acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa
    20941 tatcgatagt caattgccta atcggctggc aattgatggt attttagacg gcacgatcct
    21001 tttaccagga ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc
    21061 caaatttgat atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg
    21121 tatgacactt gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt
    21181 gaaacatata ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt
    21241 tattgctcag ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact
    21301 agctgatatg gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca
    21361 agtatcggca gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt
    21421 tgaagatcct atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac
    21481 aaattggatt actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac
    21541 agcggtatat tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga
    21601 tatcgaattc gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta
    21661 tattgcttct ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa
    21721 aaatgtgata gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca
    21781 tttggcaaat gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg
    21841 ttttaaaaag attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag
    21901 ctatatgaaa tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt
    21961 ctcgactcat ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc
    22021 tgttgcttgg tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg
    22081 tgtagatggt cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc
    22141 aggtaaaatc ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt
    22201 gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat
    22261 cattgatttg gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc
    22321 caatgtcatg gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga
    22381 gctagctaga aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt
    22441 cgatgcagat aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat
    22501 tggcagaaaa aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt
    22561 tttcagccac taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa
    22621 acactggctt atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg
    22681 gtcatttttg cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg
    22741 ggaaaagaca aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag
    22801 attgagggtt tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa
    22861 ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca
    22921 gaccagctat ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca
    22981 acagagtatc agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa
    23041 aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa
    23101 ggactgagac attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt
    23161 ctcacgcata tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc
    23221 gtaatggaag aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag
    23281 aacaagctac tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt
    23341 ttgaccacag atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat
    23401 gcagaagttc cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg
    23461 ggtaatgaag gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg
    23521 cttttacgtc catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa
    23581 actgagcagc caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact
    23641 ttttctggaa aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa
    23701 aataaatcaa aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct
    23761 atgattatga aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc
    23821 aagaagagat cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag
    23881 ctgagcgctt aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg
    23941 cccatcatat tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt
    24001 caaaatctga tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg
    24061 gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac
    24121 cgcgggggtt gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg
    24181 aatgtatgta tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt
    24241 ctttcaattg tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga
    24301 tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag
    24361 acggcggtat attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc
    24421 tgtttacttt cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg
    24481 aagcatctgt tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt
    24541 tcaatcgacg gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg
    24601 agcgttatga aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta
    24661 tcgatatcga aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg
    24721 gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc
    24781 atcatgagaa aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat
    24841 tactgaaaat caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt
    24901 atttgacatt tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca
    24961 tgcaggtcta taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga
    25021 acattgatct agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca
    25081 aagaattgac taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa
    25141 ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc
    25201 gttttgcttt tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga
    25261 tcgtccaccc agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa
    25321 tcgatctttc aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg
    25381 gctttattag tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca
    25441 aagtagatgt tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg
    25501 cacaagaact attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag
    25561 ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga
    25621 atggcctact cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg
    25681 aagagactgg cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag
    25741 cgatacggat ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt
    25801 cctttgaatc gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta
    25861 aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc
    25921 gtattatgcc agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac
    25981 gtgaatcatt caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg
    26041 cgggaacagt tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg
    26101 atgatgaaaa actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa
    26161 ttgtcgattc acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt
    26221 cattgcaaaa gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag
    26281 tagatactgg aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa
    26341 gccatcggca atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata
    26401 tcgttcgggc aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg
    26461 atatcgcatt tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat
    26521 catactgttt acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga
    26581 aaacgctatt tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa
    26641 aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg
    26701 atgagtacga gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa
    26761 gccgatattt ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc
    26821 gatgtattat tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt
    26881 gcgcctaaag gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga
    26941 gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt
    27001 ataacaacgt ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg
    27061 gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt
    27121 tagcggatag gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa
    27181 cagacatgtt gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc
    27241 acagtcaaga tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg
    27301 aactttatcg tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa
    27361 aatttttgaa cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta
    27421 taggaggaag taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt
    27481 tgccgcaaaa atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcat
    27541 actgcccgca acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt
    27601 accgcaacag tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc
    27661 cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc
    27721 cggattgatc tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga
    27781 tctagctggg gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc
    27841 attatcagca gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc
    27901 tgagattggt gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa
    27961 actagatcat ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt
    28021 cattattttt tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc
    28081 accagcagta tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat
    28141 tgctattatc aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt
    28201 gcaaaaatat attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat
    28261 cgtcacagta tttgttcaag tattctggtt ctttggtatt catggatcaa atgtattggc
    28321 accagtgcta gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa
    28381 aggttataac ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc
    28441 ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac
    28501 gatcgtctta ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc
    28561 taaattatcc attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc
    28621 aatcgtattg aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc
    28681 aattggatgg acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg
    28741 ggtagtaccg ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat
    28801 cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc
    28861 aaacaaaatg gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc
    28921 tttaagtttc gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca
    28981 gtgcaatgca acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga
    29041 cttttacatt taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc
    29101 cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc
    29161 tttattttca atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga
    29221 taaaaaacgg tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc
    29281 cacattcgca aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag
    29341 gaatctattt cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat
    29401 ggaaatcacg aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc
    29461 tgtccttttc gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa
    29521 tttggagtac gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt
    29581 cattgaaaac cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca
    29641 ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga
    29701 gtcttatggc atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt
    29761 taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga
    29821 aacatggtac tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa
    29881 gatagatctt tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca
    29941 aacaaagtaa gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat
    30001 gcatttaatg gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat
    30061 tcttggaatt acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc
    30121 ccttttccag tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct
    30181 tcttatccag aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa
    30241 gattgccgtg atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat
    30301 cggcgctatg gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat
    30361 gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa
    30421 gctgcaatca gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc
    30481 gaagactggt ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat
    30541 tttgatgata atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt
    30601 attattgcgg agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta
    30661 agttctcaca atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat
    30721 caaaattatc tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg
    30781 aggatcgtag atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca
    30841 tttgccctac aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta
    30901 acaatcgaaa atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt
    30961 cgaggaagta tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg
    31021 gtattccaag aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg
    31081 ccacagcaac agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt
    31141 tttgtcacca cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct
    31201 cttttttcgt aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt
    31261 cttggattgc ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg
    31321 cgtatacttt ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt
    31381 ctacctctag tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct
    31441 gattttttta gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga
    31501 aattgttcct gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt
    31561 caattccagt caggaacgta taaataaccc ctgtatcctt tgcggtacag gggtttgttt
    31621 tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt tatataaact atcgaacaca
    31681 gtattgattt tttctttatc ctgatcttca agctctcgaa caatatgaag ataagtagac
    31741 attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct
    31801 tgataaagta aaatagatgc atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca
    31861 atccctaatt cttttaattt tttgcgcaaa tatttgctga cggcgtttga tgaaactaat
    31921 ccatttttta agttgaaaaa aacaaaatta tgagggtttt tgatttcaaa attctcgaat
    31981 aattcttttt gattgatttt aaactttttt aagagatcaa tcgtatgttt atcaatggaa
    32041 attttcctat ttgaagtttc gttttttgtt tttccccatt catttaattt ataattccat
    32101 gttttattaa tagtgatgat ttgatcttcg aaatcgatat cttcccatgt taatcctaga
    32161 agttcagcaa atcgcattcc agtggcacca gcaactaaca cgagcatagg agaagagtac
    32221 tgcgcagaaa ggttttcttc tacgagtttc attaaagatt taaattgatc ataatctaaa
    32281 tatttatctt cttttggctt caatgaatct tttcctttga ttactgcttt tctagttgga
    32341 tcaaacggaa ttaatccttc ttctacggca tctttcaacg acgctctaat atggttatta
    32401 aacttaataa cggtagattt tacatggtct ttagcatact tatttaaaaa gcgttgatat
    32461 cccactctat ctaaatcaga aatcaatacc gctggcatat atttctttat gttcattaac
    32521 gtatcttcgt attttctata agtgatagga gatactgttc cttctttata aagttgcatc
    32581 caatcctcaa agtaatctga taataataga tttttgcgat cccctttaag gcctttgttc
    32641 aagttatatt ctaattcgtt ggcggcatct tttgcttctc cttttgtctt aaatcctgat
    32701 tttcttatct tcgtgtattt tccgtcatct tttttgtatg aaatttcgta ttgccaacta
    32761 tttccacgtt tgactaatct tgccataatt gaatcagctc tctttctctg atacaatagg
    32821 aactataaag aagcccattg tttgggtttt attatttttt gcacatcctt tttgcttgcc
    32881 ggcgcaagga tgtgctattt gtcgttttaa tggtataatt ggatgtgtat tagatcatcc
    32941 aatcagaggt gtttattatg attaaagtga tttttaaaag aataactgaa cttccagagt
    33001 ttagattatt tgttgattat tggtatgtct ggttttttat tgttgcattg ctgataggtc
    33061 tagatgtcat attagaaaga aaacttaagt aagagttgag ttaataaatt gaattcctaa
    33121 atgagatatt ttgtagccca tattttgttt gccgacatca cctaatacta tggagctaat
    33181 cgttttttct aacagaccaa aatcagataa tctatttaag ctaccatcta ttagctcttg
    33241 tgaatagcca gacatgctga tatttccagt gaaattttcg tttccacgac tcattaagca
    33301 tgctaacaat tgaatctcaa tcgatgttaa ttgattcagt acaagaagat aatactcctc
    33361 gttatcccat gaagcgttgt taattcgaag taaacaattc ttataaagat ttttatacat
    33421 atctatttta ttttgtgcct ttgatttttc tacctcgtca tttatccctt cgaatattcc
    33481 aatgagttgg tccttgttct ctaagttgaa aacatcttct ggaatttgat cgcgaatgtt
    33541 ttctaatgat tcgtttaaat ttttgtagaa tttctcgatg cgtttgaaac gcttctcgtt
    33601 tttagaccca aaataaagtg actgcacaac gccaccaata taaggaattg ctccaatacc
    33661 gccttcaact attaattcaa gatattcttt tttggttagt ttttcattgc tattatccat
    33721 agttacctcc tattttgtca tttcctttac aagcattgtg actcctgatg gcactctttt
    33781 gtataggttt tattattgtg cacaatctaa ctcttagtgg ggcgtggttc atttattttt
    33841 tacttacttt actattaatc atatcccatt gagcatagga tttttctatg ttcgatcctc
    33901 ttgaactaat ttctcctgta aaagttactt tatcgccctt tgtaaaggta tcagcattgt
    33961 ttaagtcctt cgcaaaaatt acatatgaaa ctttaggtgt tgcagaaata tcattccaac
    34021 ttttatcatc gtactctttg tctgctataa tggctattct agtagtcatt gattcgataa
    34081 cagttccttc aaaagtatat gttttaccac taatatattt agtaaaagct gtattttgct
    34141 cagtagctgg agtaatagaa taataactat ctaagaaagc accccagtca tgattatttg
    34201 tctctaaaaa ttcttcaaat tgagttaggc ttttattgct gctgttagat gtagtagttt
    34261 gagtatcaga tgatgagctt tcctgtttgt tattgtcatt tgcctcgttt gatgaggaaa
    34321 gcgagaaacc tacggtagta aaaataatcc cgataataag gagaatcata ggtgttttct
    34381 tatttgtttt tttgataaat cttacaatta acatgattaa acctataata aataaaacaa
    34441 taccagtaaa accaaatata ccgtccatta atagatcctc tttctctttt gatattgaat
    34501 attataaacc aagcaattct tttttcttaa aatcgaattc ttcttgatta ataatccctt
    34561 catctaacaa ctctttatac tttttaattt catctgcact tgaaatttgt gtttctttgg
    34621 aattattatc taatatagat tgttgcttct ctatatattt ttttaaattt tccatttcat
    34681 tccaatattt ttttgtaaac ataacggtgt tttcatcttt cgtggccgct aatatacctc
    34741 cgcgactttc gtttccgcct agcaaggtaa attgtatata accattactc atacctggtt
    34801 tttttaattg tacggctgat atatttttaa aggggatagt tttttctcct ttaatacctt
    34861 gattaactaa atttataaat ccttttctaa taattgaaat agaatcatca gttactttaa
    34921 tcaaggtttt tcctggagat tttattaaaa tctctttttc cattttttct tcctcatttc
    34981 tgtgatatga ttttttttgt agaagctcat atatgaggaa agagcccgtg ttgcagcacg
    35041 ggctttttta atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat
    35101 attgccttgc gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt
    35161 atctgagctt attctgaata gaataatact ccataaattt ttcgagatta aactgagatt
    35221 catcagtcag ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg
    35281 cttctgtttc aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa
    35341 aatgagaggc gctgaaatgt gcaagttcat gagataaatg aaaggcttct gcagtttcac
    35401 cgtatagatt ttcattcaaa aaaatgattc tgggttttgg atagtagaaa ccaggctctt
    35461 tcatctccat ataaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat
    35521 acatacaaac catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc
    35581 tttacgcatt gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc
    35641 tgataaatcc acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga
    35701 gacatcgaaa tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat tatttttcca
    35761 ctttgctaga taaccgttag aatatccaaa attaatttct aactgccgta tagatatctt
    35821 tttcctcttc gccaagtctt ttattatttc ataggtattc atcgataaaa caacctttct
    35881 aaatgtttac aaaaaaagtt tagaaaaata cgcagaaata atttgactaa ttctgagtaa
    35941 tgagctatac tatatcttgt aaacagattt aacaactaaa aagacaacaa aaaacactat
    36001 tgatatgtaa atgcagaccg ccaagaaagc tttaaaatca atgtttttat gtcttattta
    36061 attatgctct tagtatagag tattactcag atactgtcaa ttgaatttag aaaaaagttg
    36121 ttaaatttgt ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt
    36181 taatttcgaa caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg
    36241 caaagacgtt gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt
    36301 agatttggaa gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac
    36361 aatcatcaac gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa
    36421 aaaatttaaa cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta
    36481 tctaactcca gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc
    36541 aactcaacta aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta
    36601 cgaacctaaa atctcctatt tagatagcat attatcttct acagattcag taacaattag
    36661 tcagattgca gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact
    36721 aggtattcag aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg
    36781 atacacaaaa tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat
    36841 gaataccaaa tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg
    36901 atattaccct caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac
    36961 attgcagaaa tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaaag
    37021 ttcttaactt acaccatgct tgctgaaaga tttggaatta gcaaaagcta cttatcttta
    37081 attttaaacg gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc gattatcact
    37141 atgtacgaat tgtaaaggga ggaacagcta atgcaatatc tagaagcaaa aatcccaatt
    37201 ccagaaggct atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat
    37261 actggtagat ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg
    37321 tttacttcta gagttttaaa gaacccaaga tatagaaata ttatcgatat cgagaaaaac
    37381 aaaaatggat ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt
    37441 aaaacacttg aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg
    37501 aaaatttaat agagcgctag tgttcagcgc accgctaatc atctacgctt taggactttg
    37561 gggaagcag
//
LOCUS       DAYYAO010000020        36568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000020 DAYYAO010000000
VERSION     DAYYAO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.021"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHARI_000921"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHARI_000921"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8441985.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHARI_000922"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHARI_000922"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8441986.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHARI_000923"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHARI_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8441987.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHARI_000924"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHARI_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8441988.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHARI_000925"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHARI_000925"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8441989.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHARI_000926"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHARI_000926"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8441990.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHARI_000927"
     CDS             complement(8053..8523)
                     /locus_tag="ACHARI_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8441991.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHARI_000928"
     CDS             complement(8569..8994)
                     /locus_tag="ACHARI_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8441992.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHARI_000929"
     CDS             complement(9061..9330)
                     /locus_tag="ACHARI_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8441993.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHARI_000930"
     CDS             complement(9341..10936)
                     /locus_tag="ACHARI_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8441994.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHARI_000931"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHARI_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8441995.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHARI_000932"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHARI_000932"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8441996.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHARI_000933"
     CDS             15402..16376
                     /locus_tag="ACHARI_000933"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8441997.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHARI_000934"
     CDS             16546..17946
                     /locus_tag="ACHARI_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8441998.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHARI_000935"
     CDS             complement(18024..18746)
                     /locus_tag="ACHARI_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8441999.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHARI_000936"
     CDS             complement(18825..19331)
                     /locus_tag="ACHARI_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8442000.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHARI_000937"
     CDS             19454..21253
                     /locus_tag="ACHARI_000937"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8442001.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACHARI_000938"
     CDS             complement(21321..22895)
                     /locus_tag="ACHARI_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8442002.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHARI_000939"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHARI_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8442003.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHARI_000940"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHARI_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8442004.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACHARI_000941"
     CDS             complement(24672..25310)
                     /locus_tag="ACHARI_000941"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8442005.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHARI_000942"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHARI_000942"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8442006.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHARI_000943"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHARI_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8442007.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHARI_000944"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHARI_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8442008.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACHARI_000945"
     CDS             complement(29104..29844)
                     /locus_tag="ACHARI_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8442009.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHARI_000946"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHARI_000946"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8442010.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHARI_000947"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHARI_000947"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8442011.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHARI_000948"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHARI_000948"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8442012.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHARI_000949"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHARI_000949"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8442013.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHARI_000950"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHARI_000950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8442014.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHARI_000951"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHARI_000951"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8442015.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6705 g        11989 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DAYYAO010000021        34986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000021 DAYYAO010000000
VERSION     DAYYAO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.022"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..1698)
                     /locus_tag="ACHARI_000952"
     CDS             complement(130..1698)
                     /locus_tag="ACHARI_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8442016.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1702..2112)
                     /locus_tag="ACHARI_000953"
     CDS             complement(1702..2112)
                     /locus_tag="ACHARI_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8442017.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2109..3032)
                     /locus_tag="ACHARI_000954"
     CDS             complement(2109..3032)
                     /locus_tag="ACHARI_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8442018.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(3029..3907)
                     /locus_tag="ACHARI_000955"
     CDS             complement(3029..3907)
                     /locus_tag="ACHARI_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8442019.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3894..5231)
                     /locus_tag="ACHARI_000956"
     CDS             complement(3894..5231)
                     /locus_tag="ACHARI_000956"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8442020.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5234..6337)
                     /locus_tag="ACHARI_000957"
     CDS             complement(5234..6337)
                     /locus_tag="ACHARI_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8442021.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6411..7919)
                     /locus_tag="ACHARI_000958"
     CDS             complement(6411..7919)
                     /locus_tag="ACHARI_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8442022.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7885..8577)
                     /locus_tag="ACHARI_000959"
     CDS             complement(7885..8577)
                     /locus_tag="ACHARI_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8442023.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8599..9573)
                     /locus_tag="ACHARI_000960"
     CDS             complement(8599..9573)
                     /locus_tag="ACHARI_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442024.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9873..10745
                     /locus_tag="ACHARI_000961"
     CDS             9873..10745
                     /locus_tag="ACHARI_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8442025.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11203..13401)
                     /locus_tag="ACHARI_000962"
     CDS             complement(11203..13401)
                     /locus_tag="ACHARI_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8442026.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13633..14625
                     /locus_tag="ACHARI_000963"
     CDS             13633..14625
                     /locus_tag="ACHARI_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8442027.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14685..16106
                     /locus_tag="ACHARI_000964"
     CDS             14685..16106
                     /locus_tag="ACHARI_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8442028.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16099..17598
                     /locus_tag="ACHARI_000965"
     CDS             16099..17598
                     /locus_tag="ACHARI_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8442029.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17637..19031
                     /gene="uxaC"
                     /locus_tag="ACHARI_000966"
     CDS             17637..19031
                     /gene="uxaC"
                     /locus_tag="ACHARI_000966"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8442030.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19190..19873
                     /locus_tag="ACHARI_000967"
     CDS             19190..19873
                     /locus_tag="ACHARI_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8442031.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19875..20711
                     /locus_tag="ACHARI_000968"
     CDS             19875..20711
                     /locus_tag="ACHARI_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8442032.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20722..22476
                     /locus_tag="ACHARI_000969"
     CDS             20722..22476
                     /locus_tag="ACHARI_000969"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442033.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22752..23093)
                     /locus_tag="ACHARI_000970"
     CDS             complement(22752..23093)
                     /locus_tag="ACHARI_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8442034.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23161..25332)
                     /locus_tag="ACHARI_000971"
     CDS             complement(23161..25332)
                     /locus_tag="ACHARI_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8442035.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25543..26400
                     /locus_tag="ACHARI_000972"
     CDS             25543..26400
                     /locus_tag="ACHARI_000972"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8442036.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26471..27328)
                     /locus_tag="ACHARI_000973"
     CDS             complement(26471..27328)
                     /locus_tag="ACHARI_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8442037.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27313..30015)
                     /locus_tag="ACHARI_000974"
     CDS             complement(27313..30015)
                     /locus_tag="ACHARI_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8442038.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(30028..31317)
                     /locus_tag="ACHARI_000975"
     CDS             complement(30028..31317)
                     /locus_tag="ACHARI_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8442039.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31467..32513
                     /locus_tag="ACHARI_000976"
     CDS             31467..32513
                     /locus_tag="ACHARI_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8442040.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32515..34668
                     /locus_tag="ACHARI_000977"
     CDS             32515..34668
                     /locus_tag="ACHARI_000977"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8442041.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        10286 a         7193 c         6297 g        11210 t
ORIGIN      
        1 aacaaaaaat aatctaaaaa cattgatgca taagattgat aacttttttt acataacaat
       61 atatacaaac aaaaaaattc actaactagt gaaaataagt tagtgaacct catttattaa
      121 agaattaaat tacagatgat cttttccatc atacttgaaa aactcaaaat cagcataact
      181 tcctcttttt ttatttagat catgcactgc tatcccgata aaatttccag taaaccctcc
      241 tgagagtata cacatgtttt tttgcggacc tactttcttc cattcttcag ttgatacgtc
      301 tttcaaatga aactgtgctg tctctttctt tacagtgact ttcaaaaaag tcttcttttc
      361 tgtgagtgga atcgtccaat cttctatttg tgctactccg ttttctgatt ctatcatacg
      421 taggcatttt cccttgtcct cattccaagt aacataacaa taaatataat tggattcatt
      481 taagtacagt accagtccag ccatctgatt aaaattatta ggcatgtatt caaatgctgt
      541 ctccgcatca aaagtaaaat ccgtttgccg aatagctaac aaatgatgtt cgaaggtaga
      601 ttgaagagat tcaccggaaa tcagacgtaa aaaacctggt ctacattttg tatcacacca
      661 atcagcattt ggcaaaatgc gtctggcatt ccagttcttc tttagctctc cttcaaatgt
      721 atccataaac tgatgatctt tttcttgttg tacctcttca tttgtctcaa tcattatttc
      781 ttccatcgga gttgtctggc tatctttaag tcttaaccaa tcgtcggacg tccaatacac
      841 ttcttgaata gcggtttctc ttcccaaaat agccgcaccg tttaatggtc tggtacaaag
      901 atatgtcatg taccatcttc ctgacggggt ttgtactaag ctcccatgtc ctgtacattg
      961 caatgtagaa gcaggtttgt cactagctgt cagcatagga aaaccaggat caagctcgta
     1021 aggaccagta atattctttg ctcggcaaac tgtaactgaa tgtcctcttc ctgtaccgcc
     1081 ttctgccgtc atcaaataat aatacccgtt acgaaaatac aaatgtggtg cctccgtttt
     1141 cgctaattct gtacctgcaa atattttata gatgggacca tcaagcttct tcttctctgc
     1201 atcatattct tgtaatacga taccgctgga tttattcggt gtgtccagac gataatccca
     1261 tagttcattg acaagccatt tttttcccgt ttcaagatca tggaacaaag aaggatcaaa
     1321 gccgttacta tttaaataaa ttggttcgga ccaaggacct gtgatctcag atgcagtgat
     1381 caaataattg tggacatctt taaacggacg gcctgcactt ttgacatctg tgtaaacaag
     1441 ataaaacaat ccatcagcat aactgatttg tggcgcccat atacttccat ttgtaggatt
     1501 tccttgcaaa gatacttgag tggtcaaaat atccgttgta tgtgaccagt tgactaaatt
     1561 tttcgactca tatactcgga ttcctggaag ccattcaaaa gaggagacaa taatgtaata
     1621 ccgctctttc actcttaata tattaggatc tggattgaat cctcttaaaa taggattttt
     1681 cactttgata gtctgcatga ttcaggctcc ttttctcatt cttactcgat ataaactgaa
     1741 tgccccagct gcgaaataaa aaaatgcaaa aggaaacgca ataaactgag tgccaatcaa
     1801 caatatgaat cctgttataa aatacaaacc agtggcagtt gttaagtatt ttcctttagc
     1861 agtcaaaaac aatgctactg ctaaaaaagc aagtgaaata aacaacacaa cggcaaacag
     1921 accagtcatg tcctgtaata atccaaatgt attttctaaa gacaaagttg aatgatctac
     1981 agcaagcgac tgataacttt ttttaaactc tgacgcagac atattactta aagtaactgc
     2041 taacccgcca aagaaaatca aagatagact gccaccgacc attcctagca atagttccca
     2101 ttttcttttc atttctgaac cccctgcttt tctaaatgta tccattcagg tcttttgatc
     2161 gatgtttcat aattgttatt acgttcagag aatttgacag tctgttcatt tttcttgtca
     2221 tcccaattat cccaaccagt tgggtgaaca tgcccaccta tcttacacgc ttcaaataag
     2281 acatttgcat acggacgcca cggtcttcct aaatagacgt ttctagtatc aggttctgca
     2341 gtcaaggtac agctttcaaa acgataccct gttttttgat ttttcggcgt agatgctgct
     2401 gtgatgtatc caggattttt actggctaag ctatggatat gacactgatg aaaaacggca
     2461 tctgccccac caaatatgaa atccaccgtt ccttcaatcc agcaatattc ataggtctga
     2521 cgacaatact caggattggc agaagccggt gtacaaaaag gagtaccatc tttttgcaaa
     2581 tcaggcaacg gacccgtgca taaggtatcc tgataaccag atagtcgaca gttttttaaa
     2641 gatacgcgat ctcctgaatt gaataaagca acagcctgtc ctaccttttc tcctggacct
     2701 gctgtatttt cgatgtgaag attttctaat acgacattcg atccttctaa gaaaaaagta
     2761 gcacttcgga aagtccctaa ctggctaccg tcaagatact cctgttttgc aaaacgatta
     2821 ccggagataa ctactgttcc tgctcccaca agtttaaaat tggataatct gctttctata
     2881 tattcatcat atcttccttc ttttactatc aagacctttg gatggctatc cggcaggcct
     2941 gccaaataat cgattccttc ttggatagtt tcaaaatccg tcggggtatc tatagcattc
     3001 tttccaatta tcagtttgtc tttcatcctc ataaatgctc ccttatgaat tgtttccaaa
     3061 tatttctcat ttcttgtgtt tccatatgac cgcctgttaa agaaaaagaa gaaaagtttt
     3121 ctggactacc cattgattga tactttttgt ccagcccttt tctcagcttc agtacgcctt
     3181 cgtccaagca catcgtatcg ttttttccaa ctaagctaag acgagggcgc ggagcgattt
     3241 tttcttggat tgctaacgtt gtataagctg tcaatagatt tggcacatag taataaaacc
     3301 catgatgatc taggcggcga tgtagcaaaa gtatttcgat gtccacctgt gccgctagat
     3361 caatacaaac tttgacacga gaatccaatg ctgtcaccca ccagcttaat aagcctccca
     3421 tagaaagacc aatcgttgcg agccgttgag tatctgtgtc ttcccttgtc tccaaataat
     3481 cgatcaagct gatcacatca taaatacgca ttccccataa cgtttttcct gttaataaaa
     3541 attgtttgaa caactcgctt tcagaaatac ctccgcgttc ttcaaatccc caagcatcaa
     3601 tcgaccaaac agcatatccc attcctgtca gtacttctgc aaaagatgga gaagctaagt
     3661 aaggagcacc atggatcaat tcacttttcc cgactgaaaa atctccgccg tgagaatgca
     3721 ggtaaaggac cggcggaaac ggtccttctt tttctggata agcatagagg gctggtactg
     3781 tttccaattc atttaagtac aattgatagt attcaagcat aaaaccgttt tgtttttctc
     3841 ggtataatcg ttttccacta ggggtatgtc tgctagatgg agcacctaaa aaatcattca
     3901 actgcataag caaagttttt acgaacaacc agttcatcca tattttctga ttgatttgat
     3961 tggaatttct caatactgat ttctttagta ttttctaata gaaatgctgg tccctcgtga
     4021 ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa agccaatata aaatccttgt
     4081 ctgctcattt cctctattcc tgtcatcata gcaggaacac cgggaactgc atcttgggcc
     4141 atcgagacat cgatattgtc aaatgtgatt tcttgtatga attgttccgc taatccataa
     4201 ataaaacctg cagaagcatg gacattcctt gccgtgatat tcgaaaaatg gatccttctg
     4261 aatgctggtg tccgttcatc tatcggataa gccttttttt cccatacata cggttctttc
     4321 cctcgcggac cacagaaata ataaagattc agtataaatg gacacatcac attatccatg
     4381 actatattgg atactcgaat atcttcaacg atcccgcctc gacctcgtct agatttcaat
     4441 cgtattcctc gatcagtttc ttggaagata caatttgaaa tggtaatatt acgaatacta
     4501 ccactcattt cacttcctaa tacgacaccg ccatgtccat ggaccatcgt gcaatttgtg
     4561 atcgtgatat tttcgcaagc gatcctttcg tacgtatctt cagtccctgc cttgattgca
     4621 atacagtcat ctcccacgtc aatatggcaa ttactaatac gcacattttt gcatgattct
     4681 ggatcaattc cgtctgtatt tggtgaatcc gctgggttca agatcgtcaa gttatcaaac
     4741 gttgcattac tgcaaagaat cgggttgatc gtccaacttg gtgactggat caatttaata
     4801 tctttcacag taattcgatg acagttatgg aaactcatca atttaggacg aggataagcg
     4861 agattatctg gttcatttct aaaagtatgc caccattttt ttccatttcc atctaatgta
     4921 ccgaaaccag tgacagagat gttctctaca ttttgagcat agatacagga agcataaact
     4981 tttcgatgga ctccttccca acgtgaatga acaacaggat aatcttttgg atcgtcagag
     5041 aatttcagaa ccgctcctgc gcttaaatga agttcgacat tgctttttaa aaataaagct
     5101 cctgttagaa attcacctgc tggaacaact acggtcccgc ctccgtcact tgctgcagca
     5161 tcaatggctt gttgtattgc ttcagtattc aattcatcaa ttgatgcgcc aaatgtcaaa
     5221 atatcataca tatttataat gcctccactt caatcaatgc ttgcaagaat gcaccaacgc
     5281 ctttttgatc attacaaata attggttcac tgatgtagta agcatatgaa ccatctcgct
     5341 gatctgctcc gcctaaccct gctacttgac agtttttatt caagttcagc cagccttctt
     5401 ttgtttccag aacaaattca tcaagtaatc cttgatgtga tttcttcact tgtggaagcc
     5461 attcttcttt gttcagcagt cccaaacgta ttcctttagc cattgcgtaa acaaacatac
     5521 tactgccgga agcttccaga taatttcctt tccgttttgt ttctgttgtt acttgatacc
     5581 atgtcccgct gttttcttct tgatatggaa gtaatgcagc cagtgtcttt tctagaattt
     5641 ctttcagcaa agaacaatct gtttcttcat gaagtaattc ataggtatct gctgcagcca
     5701 tcaaatacca gccaatcgat cggctccaaa aatgatggga tagacctgtt tttggatcag
     5761 cccatggttg gaccttcttt tcatcccatg catgaatcag caaatttgtt tttggatcga
     5821 atagatgttt ataactgatt tcaaactgtc tcaaaacatc ttggtaatct ttttcttcac
     5881 taaaattctg gataaattca gcataaaaag gagaacccat atataagcca tccaaccaaa
     5941 tttgataagg ataaatttct ttatgccaaa aagctccttc ggatgtgcgt ggatggtttg
     6001 ccaactgctg ggaaaggagt tctgctgctt ttttatattt ttcttcgcct gtttctttgt
     6061 acaataaaaa gaaaagcttt cctgtattta catggtcgat attgtattct tcaacgctgt
     6121 agccacggat cgttccatct tcttgcacga aatagtcgat attgtctttt atgaaatcaa
     6181 aataaatttt ttcttgggtt cgttcccaaa gtaattggaa ccctttaagt acgacgccat
     6241 agtcataaga ccattttcct ttataccact tttcttcata taagcgaggt gttctttcca
     6301 tgatactttt tgctaatttc tttgaccagt tttccattat ttgctctcct ttaaaaatta
     6361 aaataggatt tgggacaaat accaagaagt atttgtccca atcgtctata ttactgtgcc
     6421 gctgctttgt atgcttcctc gtactcttgt gtgattcgat cgcctccttg tgctttccag
     6481 tcttctacag cttttttgaa tccatcttcg tcaagctgtc ccatgatgta ttgcaccgta
     6541 gcatcaatga cgattttttc aatttctgat cctacttcat tgttcgtcgg tgaatccaat
     6601 ggcacagaag gatccattac agcaaattct tcattttctt tgatcaattg attagagaat
     6661 tctttttcag ggtctgtatc tttgaaagag aacgtcactt cactaggacg agagcttgat
     6721 aacggttgca cctcttgttg ccaaagatcc atatcgataa attcgataga accatcatca
     6781 ttcaattcat aatgcgttcc ttcgataccg ccagtcatca atttatacac atcttcttct
     6841 aaaagatcat caacaaattg aagtaatttc ttcaattgtg cttcgtcttt tacttgagat
     6901 ttgggaaatg ctagcaagcc gccaatacca tttccttctg accaaacatg ataaccgccg
     6961 ccttcaccat ctgagatctt gtttacaggc actaattcca tatcatcttg aataccttga
     7021 cccatcgtct tcagattctt gatatccacc attccagtgt agattccagt tcttccttgt
     7081 gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa agtcttgtgc caaatagcca
     7141 ttctcaaata gatctcttga aaaattcaaa gctttcatat actcttctgt atcaaattct
     7201 ggagtgaatt tgccattatc gtctacagcc cagccatttg gtgctccaaa ataagaagtg
     7261 aaaagtttaa agctggtata acgaatatca ctaggcgcac gatcgccaaa accaactgtg
     7321 tcgtccacac catttccatc aggatcgttt tctgtaaatt gtttagctac ttcataaagc
     7381 tcatccatgg tagttggaac atctagtcct agattatcca accagtcttt tcgaatgact
     7441 aagccagcac gagcgtagtc tttttgtatc ggtacgccgt acaaggcacc gtcgatactc
     7501 gcagcttcga ttctatcttc agaaatttga cttaaatttt tatagtcttt taaatattct
     7561 gatacatccc agaatacgcc tgattttaac gcattgcgga cagaagagtt tttcaacatc
     7621 gcaatttgaa gtgtaacgat atctcctagt tcatcagaag ccaatgcagt tgttaatctt
     7681 tcttcttttg aagcatcagg aatccagttg aatgtcagtt ccgtatcggt tttttcttcg
     7741 atcaagttca caatcgtgtc tgtaggcggt gaagcatgat gcaaaatatt taaccaagtg
     7801 attttagctt ttccatcttc tgtctctgaa gaactgttac ttgaactgct gccgcatgcg
     7861 gcaagtgcta gaattcctgc aaaactaaga cctaatttta cgagcgttgc ttttttcata
     7921 aattttctcc ttcactgttt taataatatt tttgttttct tctgtttttt ctgtatatga
     7981 gttcttccat tccaaatggc aaaatataga atttgctgtc cacgtgactg cggaaaagaa
     8041 gagactgcca ccgataaagg ttaaaacacc tggcattgtt tggatgatca tcagataaat
     8101 ccctaccata cttaatatca acactaatga gtaatgaaga tgtgagaatc cgaataaaaa
     8161 tgccattttc attttgaaaa aatatccttt ccaatcataa tgagccatga ttgggaaaat
     8221 atagagtgct gagagcacat atatgaacgc agcaatatac aaagcgatcc taagatacca
     8281 attgtgtatg aaagaaagat ccacaagtaa aacaatcccg atggcgccat atatccagct
     8341 catcagcaag gtttcacgat agttttcttt aaaatatgtg aagtacgttt tcgtcaaagg
     8401 aaacgtttct tttgagcgca gccattttct aatcactgca accatcgcat acgatgcagg
     8461 accgaaagta aaaactccca aacctgcgat gactaaaccg ccccacagaa aattcaaata
     8521 aaccaactgt aaaatacgat ttagccttcc gtttatttta tctaatgact gcagcatatc
     8581 tttccctctc tttctatctt agtaaacgcc gtcttctcct agctttttgg caatcttatt
     8641 agctaaaatt accataaaca atcctacgac ccctttaaac aatcccattg ctgtactgaa
     8701 acttaactgt ccattcttca atccagctgt ataaatatag gtgtcgaaaa tttctgcaac
     8761 gcttcgattc aatgaattca ataacaagta catatgctcg aaccctaagt ctaatgtctg
     8821 accgattttt agaatcagca aggtaatgat tactggacga attgccggca atgtaacatg
     8881 ccatgttttg cgtaagcgtc ctgctccatc catttctgca gcttcataca actgtgtatc
     8941 gactgcagtt atcgctgaaa ggtagatgat cgtagaccag cccaactctt tccagatcac
     9001 ttgtccgata tatactgtgc gtgtccattc aggggatgtt agaaaactga ttttttcaaa
     9061 tcccattgtt gcaaggaatt cattcaatac ccctccgtca acattcaaaa atacataagt
     9121 aatcgaaaca atgattaccc aagacatgaa gtgagggatg taaatgatcg tttgaatccc
     9181 gtttttcaat cgtttgttcg ttacttcatt taatagtaaa gacaaaatga ttggcatcgg
     9241 aaaaaacacg aagatattta atccgaacaa gatcaacgta tttcttaata atgtaaagaa
     9301 tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg acccaaggac ttcccattat
     9361 tcctaaaaat ggttgataat cttgaaaagc aataacgagt ccccccatag gaagatacct
     9421 aaaaatcaag aaataaagga ctcccggcag aatcatcaca tataaaaatt tattcgtttt
     9481 gattcctgca attatttttt ttcgtcttct gagcttttct tcttttttcg atagcgtcag
     9541 ttgaaatggt tcttgtttct ttacactttc cataagcccc tccttagttt tgtgaatgtt
     9601 tgctgcttat gacttcatga tagataacta tctgaattac gtatataatc atttttaccg
     9661 taagtaaaaa cgcttacata gcaatgattt ttgtgctata caaaacgata aagtgtattt
     9721 tttcacaaat aaactacgca tagcaaacct tctatttttt tctatctgac acttagcata
     9781 ggaaatgatt atatacgtaa tctttcaccg atgataaatt gaacatatag aaacagaaac
     9841 atttaattta ttttgaggag gagcctatct atatgaaaaa gaaagtctct ataggagaaa
     9901 gaagttttac acttttcaac aatacgtttc taattttgct agctttgatc tgtatcgttc
     9961 cttttttaaa tatcatcgct acatcttttg cttctacaca agaagtcgtc gcgaaaaagt
    10021 ttatcctttt tcctactacc ttttctttag acgcgtatcg ctacattctt tctacgccga
    10081 ccatttttag agcacttgct gtttcgattg gtgttacagg tgtcggaacg atcgtcagta
    10141 tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa gtatttgttt ggtcgaggtt
    10201 tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg ggggatgatt ccaacgttct
    10261 tagttgttcg ttctctaggg ctggtcaatt cttactgggc catgatcctg cctgtggctg
    10321 ttaatgcaat gaatatgatc attatgagaa actttttcca agccttgccg gatagtttgg
    10381 aagaatcagc aaaaatggat ggttgcacag attttggtgt atttttcaaa attatgctgc
    10441 cattagcgtt accgtctatt gctactatct cgctttttta tgctgttact tactggaaca
    10501 cttatatgac tgctattttg tatatcaacg attcatctaa atggccgata cagattcttt
    10561 tacgccaaat cgtcattgta tcaagtggga tgcaagcaga aagtagtgcc gttgatgtga
    10621 ttccaccagc acaaacagtt aaaatggctg ttatcgttat tgcaacagtg cctatgctga
    10681 ttgtttatcc ttttgttcag aaatattttg tcaaaggagc actcgtaggt tctgtcaaag
    10741 ggtgattatg tactgaacgc aaaatggtag ttgtgtcaga agtggtcagc ttcaagaaat
    10801 aagaaggcat ttacgaaaat tgcttcttaa atttttgtga aatttcggct tatttttcga
    10861 agaatctact cctggaacac cgtgtatcag aggttgtgaa agagacgttc agccttgagc
    10921 gatgataaaa actctcacaa acattgcgac gagtaaccca caggagcaat aagaagcgat
    10981 tttgaaaaat agcttcccat atttcacaaa attatgcctt attgtcgata gcctgtctct
    11041 tgaacgccat ttattcgtaa aaaagaactg tgacaagact tttgtcacag ttctttttgc
    11101 gtgcttagcc ttgaaattta actatccata aatacaaata ctagggttat tctaacttct
    11161 tttattgctt gaatcagtcc acaagaaaaa cagcctcttg attcacaaat atttttttcg
    11221 atattctccc ggcgtaatcg attccttctt tttaaagaaa cgaataaaat tttgcggatt
    11281 tcgatattgc aatctttctg caatctcttt tacggagagg ttagtctctt tcaaccattt
    11341 ttttgctacc tctaaacgat gattttgaac aaaatcacca aatttttctc ctgtttcctt
    11401 tttaaataca ttacttaaat agttagggtt ataatgcaac ctatcagcga tcacatctaa
    11461 agacagatca cgatcatatt ctgtgtagac gatatgcata atattctccg acaaattttt
    11521 aaattcatga tcagtgattg cttgagagca ttctgtgatt ggcagaacta attgttctaa
    11581 aacaagtttt tctagttctt tgggatgata attgttcagt gcttttacat aaatttgttt
    11641 catgttatca aagttttttg aatctactcc caataactgt cctaattgaa cgagttcatt
    11701 gatcaagcgg accgaaacta cttcccgact caaaggattt ttattttgtg caaacaatgc
    11761 atcaatcaat tcatgtacta aattcttcac ttcctgattt ccagaacgaa tggcttcaaa
    11821 caatcgattt tgttcttcta tcggatatct aataagtgtt ttttcatgag cagcaggaac
    11881 gatttcctga taaaaaataa ttgaacttgg accagtattt acacgataat acagggcttc
    11941 ttttgcccga tcaaccgatt gcttgctttc attcagcgtt tcgaaccggt tactgatgcc
    12001 tatactgata gttaaattca agtattcttt tacacgtttt tgaatgagtc ggcaatactc
    12061 catgactttt ttgcttgctt ctgcatcatc tttatccatt cgataaatag tggcttgcat
    12121 ttcttcattc aaaacgatcg gcagcattcg attttccttt ggcacaattt ccgtgatcat
    12181 gttgttgatc gctaaaagta gcaaatcacg ctccccaccg tgactatctt ctaacagatc
    12241 gatttgaacc aaagctgtat aatagcagtc attttgagaa ctataaccaa attgctgcaa
    12301 ccgctgatct agttctcgcc tttctactcg atttctaaat aaactaagta caaatagcgt
    12361 ttccagctgt ggcttttggg taactaagtg tgcacttaac gcttcttttt ctcctatgat
    12421 ccgactgact gattgagcga ccaaatctag ttctttccca ctgaatcctt ttgatttaag
    12481 attcagacgt tcctgtattt gagaaatcgg acgggcaaat ctgtccgaaa ataagtagga
    12541 aagaagacca atgagtccta ccagtgttaa agacactgca actagcccga ttctcagatt
    12601 gcggatcatt gcgccaattg cactagagtc aacttctaat ctgtaaatcc aattattata
    12661 atctgatttc agtactatat acgttttatc tttttctctt atcactttca gctgattgta
    12721 atctagatcc acgtccgtta atacagttgg ttgctcttta ttttttactg tctgagtagt
    12781 aaaaaggttg ccaacttcgt tttcaataga aagtatgtct tctccacttt caccaacaac
    12841 ttgttctatt gtatgtttct ctatcgtagc aattcccaat gcatattttt ctttttcaaa
    12901 cattggcaaa gaaatcacca tttcgattcc tttgtttttc ttttgccaaa ataaattatt
    12961 tttgttattt atgtacttct gttgatactt ttcaacttcc tcttcggtca gttgactcaa
    13021 cgatccattg attactcccc atttaccagc caaactgact aaatcataat tattatcatc
    13081 cataacaatc gtttctataa aatttagttc tttcctgatt tcagaataag tggagtaatc
    13141 tgtatatgtc agcggatgct tgaatatgtc cgagaaattt ttggtcgaac catacgtaga
    13201 aaacgaatat tcaatcgttc taattttcca ttcaacgtta gttttaactt gcctgagcaa
    13261 attctttttg tctgttaaat aactcgattc gatcgattca taggtattct ggtaaatgaa
    13321 taaactaaat cctatgacaa gtacggctcc taataaaaac atcggtaaaa atattttata
    13381 aaatactgta cttttcttca ttttcttcct ctcctccttt taaaataaaa ggctttcatt
    13441 tttctgtaaa attgatactc atctaattaa atgagcattc cccgctttca agaatagcat
    13501 aattaaaaat aaaaatccaa gtaattttta acgtgtgcaa tcttttcata tcaaagatct
    13561 attgacttat cgtagttatc gtaatatgat gaatgcgtta acaaaaagtg aaaaagtaag
    13621 gaaaatgatg gaatggtaac gatcaaagat atcgctaaag cagctggcgt ttctcatacg
    13681 acagtttctc gtgctttgaa taacagcagt ttaataaaaa atgaaacaaa acaaaaaata
    13741 cgacaactag ctaaggaaat gaattattcg ccaaactata gtgcaaaagg tttagtgaat
    13801 caaaaaaaat atctaatcgg actatttttc tcaagtttgc ataaaggaac ttcatcaaat
    13861 ttcctagcag attccattgc aggcatccat tcagtattag acacaaatta cagtttatct
    13921 gttgaaagta tcgatgttgt tgatttaaca gaaatcaacc tgcaacgcta cgatggactg
    13981 atcgttatga gtcaaagtga tgatgatcag cctttcatcg actatctcaa agaaaaagaa
    14041 tttcctttcg ttgttgtcaa tcgtcacatc aatgatccag cggttatcaa cgtcactgcc
    14101 gatgatgcgg ccggtgttat gcaggcagta gattacggta ttggatgcgg acatacgaag
    14161 atcgcttata ttggcggaaa gaaaaatttt cgttcgacaa aagaacggga aaaaggggta
    14221 ttgaaaagtc taaaaaaagc gagcatccct gtcaatccag cctatttttt aacaggagat
    14281 tacagcttag aaagcggcct ccaaaacatg gaatacctat tatcactttc tgacctgcct
    14341 actctggttt tttgcggcaa cgacgacata gcgattggtg cattgagagc tgtacattta
    14401 gctggcctcc gtgttcccca gaacatttct ctgatcgggt tcgatgattc tccagtcgtc
    14461 tcctatctca atccgccgct taccaccgta cgtaagcctt tgaaagaaat gtgtcaagaa
    14521 ggaacaaagc ttttactaga tttgatcgaa ggcaggacga tcgaacaatc taaaattgag
    14581 ctctccacct tgttgatcct tcgtgagtcg atcaattact tgtaaactac aagtaatttt
    14641 atataaaaat gttaacgtgt gcataacaca gaaaggaagt ttatatgaaa aaactgacaa
    14701 aagaattagt gaaaaaaaac gaagcacctg taaaagttct tcagtttggt gaaggcaatt
    14761 ttatgcgtgg atttattgat tggcagattc agcagctgaa taaccaacaa ttgtttaaag
    14821 gtaatgttgc aatcattcaa cctttagaac aaggtcttgg aaatatgatg aaccagcaag
    14881 ataatttata tacggtcatt ttacaaggac tcagcaatgg cgaaatcgtt gatacttccg
    14941 agattatcac cgtagtagaa caaatgatca atccttatga tgaatgggat gcttatctat
    15001 ctctagcaga aaatgacgac ttggcctata tcgtctccaa tacaacagag gcaggaatcc
    15061 aattcgtgga gactgataat ttaacagata ctcccccaca gagcttcccg ggcaaactga
    15121 cagccttttt ataccgacgt ttcaaattaa acaaagctgg ctttaccatc attccttgcg
    15181 aactgattga cagaaacggc gaacgtctaa aagaaatcat tttacgctac gcagaaaaat
    15241 gggatttaga acctgctttt gttgagtggt tagaaaaaga aaatgttttc tgttgtagtt
    15301 tagttgatcg catcgtccca ggttatcctc gcgaagatgc aggtgttttc aatcaacggc
    15361 ataactatga agatcaattg atggtcaaag cagaaccatt catgctttgg gtgatcgaag
    15421 ggcctaaaga attggagaat caactaccat tcaaacaggc tggcttgaat gtcatcatca
    15481 cagatgatat gactccttat cgtgaacgta aggtgcattt gcttaatgga ccgcatacag
    15541 cgatggtccc tcttgctttg ttggcggaat tggaaactgt agaagaagta atgaaagatc
    15601 ctttattctc cgcatatgtt gatcagttat tcaatctaga attgattcct atgctttctt
    15661 tacctaaaga tgaattagcc atttatgctg accaaatcaa agaacgtttc ttgaatcctt
    15721 ttgcccatca caaattagaa gcgatttcat taaatagtgt ttcaaagttc agcacccgtc
    15781 tgcttccagt gttcaagaag tatatcgaag aacaaaatca ggtaccaccg ttaatcactg
    15841 tttcactagc tgcgctactt cttatgtatc gaggcgatca agtaaagcca catgatgatg
    15901 aaaagacgat aagcgaattt actgatgctt ggtctgataa tggaacagct attcctcgat
    15961 tattgcaaaa cgctgcgctt tggggagaag atttgtctca aataccgaat gtgacagata
    16021 cagttcaaga aatagctgca aagatcgatc aaaaaggtgt ccggtcagta ttacaagaaa
    16081 tgaaaggaac gatggtccat gcttgaaacc ctgcaaccaa caatgaaatt aaatcgacga
    16141 gacagtgtgg tcgtcgcatt aacccctatt ccaaaacaaa cacaactgac gattgatcag
    16201 caaacgatca ccactcttga agatatcccg caaggtcata agattgcatt gggtgatttg
    16261 aaagctggag acaacgtgat caaatatggc tatccaatcg gtcatgtgac gacagacgta
    16321 actgccggtc aatggctgca cactcataat gtaaaaacca atctttctgg tgaattggat
    16381 tatcgatatg aaaaagatgt ccatccaagt cattaccttt ttgagaaccg aacatttcaa
    16441 ggttacttgc gaaaaaatgg aaaagtaggg attcgtaatg atttgttttt agtacctacc
    16501 gttgggtgtg tgaatggcat tgctgaactg atcgtcaaac agttcaagga aaaacatcca
    16561 gatctgggac agtttgacca tattacgatt ttaaagcatc cttatggctg ttcgcagctt
    16621 ggaaaagatc atcaaaatac tcgagaaatt ttagcggacg cagtaaatca tcccaatgcc
    16681 ggaggagttt tagtatttgg attaggttgt gagaacaata cagttcctga attcaaaaaa
    16741 attttaggtg aatatgatga agaacgagtg aaatttctag tggctcaaga tgtttacgat
    16801 gagatcgaac aaggtgtggc tctattagaa gagcttttaa cagcggcgga aaatgaccag
    16861 cgcatatcag tcccgctctc taagttgaat gtcggtttga aatgcggcgg ttctgatggt
    16921 ctatcgggaa taactgctaa tcctttactt ggtgcatttt ctgactactt gattgctcaa
    16981 ggaggcagca cgatcttaac agaagtgcca gaaatgtttg gcgcagaaca agtattgatg
    17041 gcacgagcgg aaaatgaaga agtttttgac tcaatcgttg acttgatcaa tgacttcaaa
    17101 cagtatttct tgtcttacgg ggaaccagtt tacgaaaacc catctcctgg taataaagca
    17161 ggtgggatca ctacattaga agataagtct ttaggctgca cgcaaaaatc aggaagctct
    17221 cctgtcgttg atgtgctaca atatggcgaa aaaatcagaa aaccagggct tagtttattg
    17281 caagctcccg ggaatgatct tgtagctgcc tctgctcttg cttcatccga ttgccagttg
    17341 gtcttgttta ccacaggaag agggactccg tttggctcat atgtcccaac ggtcaaggta
    17401 tctacgaata caactctttt tgaacgcaaa ggtcattgga tggattttaa tgcaggcgtc
    17461 ttattagaac aaccaatgga agtaatctta gaatcgtttg ttcaaaaaat catcgctgta
    17521 gctagtggag aagaaacgaa aaatgaacaa aatgacgttc gagaaattgc catttttaaa
    17581 aacggagtaa cactttgatt ttcataaaac agtataaaaa gaaagaagga agatttatgt
    17641 ttttatctga tgatttttta ttaaaagacg attgggcgaa aaagctttac cattcctacg
    17701 caaaaaacat gcccattatt gattatcact gtcacttaga tccaaaagaa atttacgaaa
    17761 acaacaattt cagcaatctg acagaagctt ggttaggagg cgatcattac aaatggcgct
    17821 tgatgcgagc ttgcggcgtg cctgaagaaa aaatcacagg aaatgcttct gattttgaaa
    17881 aatttttagc ttggtgtcaa accgtaccaa aaattatcgg aaatccgctt tacagttggt
    17941 cacatcttga attgaagcgt ttcttcggta ttgacttgtt gataaatgaa gaaaatgcag
    18001 agaaaatttg gacacaagca aatgaaattt tggcagctcc tgattttcgc cgtcgggaaa
    18061 tcgcaaaaaa atcgaatgta gaagtgattt gtacaacaga tgatcccgca gacgacttgc
    18121 actatcatca gttgcttgct catgaagaac cagatttgaa ggttctacct tcattccgtc
    18181 cagataaagc actcaatatt gaaaaagaag gatttaatgc atgggtcaca ctactggaag
    18241 aagcagctga ccaaacgatc accacctatc aagaactgat agaagtctta gggcagcgga
    18301 tagaatactt ccatcaacat ggatgtcgtg tttccgacca cgctttagac atcttacgct
    18361 ataaagaagc ggatgaaaca gtattagaag agatttttca aaaagcacga gctaacaaac
    18421 tattatcttc agatgaaatc gatgcttatc gtacagaaac actgatacat ttgacccact
    18481 tctatcattc tcataattgg acgatgcagc tgcacatcca tgcttatcgt aactgtaata
    18541 cacaaatgtt ggaaaaatta gggcccgata ccggctatga tggaatgaat gatctttctt
    18601 tgactattcc tctgcaaaaa ttattgaacc gtgcagaaga aacggatcag ttgccaaaga
    18661 cgatccttta ttcattgaat ccgaatgact atcctgcgat tttagctttg atgggctgtt
    18721 tccaaaaaga acagaatgga aaactgcagt taggttctgg atggtggtac aacgatactc
    18781 gagctggtat gcgcgatcaa ttgacacaat ttgctgatgg tagtgctttg gggaattttg
    18841 tcggcatgct gacagattca agaagtttcc tgtcctacac tagacatgaa tatttccgtc
    18901 gtgttctttg tgaattttta ggcgaaatgg tcatgcgagg agaagcacct gaagatgaac
    18961 aacttttagg aagtctagtt caagcgatta gttatacaaa tgctaaaaat tactttggct
    19021 tttttgccta attgattaat agtaacagta acgaatcaaa aacaggatag tgaaataacg
    19081 gaatccgttt atccacgttt tagagaagaa gacggaccaa atttgctccg tcttcttctc
    19141 ttgctgttct gcttcattgc ctataaacct ttatgaaaga agagataaca tgcctactat
    19201 ccatattgcc ggtgattcta ctgcggcaga aaaagatgaa aacaagcgtc ccgagacagg
    19261 ttggggagaa aaaatctctg cttatttttc acccgagatc cagatcaata accaagcaaa
    19321 gaatggacgc agcaccaaat catttatcca ggaaggaagg ttagcccgtc tagcttcaca
    19381 gtttcagccg aatgatttct tatttgtaca atttggacat aacgaccaga aaatccaaga
    19441 agaaagaggt acgcttccct tcggtagcta tttaaaaaat ctgcagatat tttttgaatg
    19501 cgcaaaaaaa gcgaacgtcc aaatggtctt actgacatct gtgacgcgac gagattactt
    19561 agaaaacgga acattgaatc cagatatact tggagactat ccaaaagcga tgcgtgcatt
    19621 tgctgagaaa caccatattc ctcttttaga tgtgttctca agatcacaag aattgttcca
    19681 gaccttctca aaagaagaga caaaaaaatt ctacctgcat cttatacctg atacttgtaa
    19741 aaattaccca gaaggactaa aagacaatac ccactttagt ccagaaggcg ctgataaagt
    19801 tgcccgaata attgttgaat tgataaaaga atcccgtctg cctcttgcta attatttaca
    19861 aaaaggagtt tgagatgttt ccattaaatt taggcatccg tgcccacgat ttagatgttc
    19921 gaaatcgtga agaaatcgtt caaaaagtat cacagcttca cttgtcccat atccaatttg
    19981 caccacaaaa agcatttcct gaacaactta aattaactga catgacactt gggagtgctg
    20041 cctattttgg tgaatacttc caaaaaaaca atatcgaact ttccatttta ggatgctata
    20101 tcaacctatc cagcaaaaat caagaagttc gtgcacaagc attggcaact ttcaaacagc
    20161 aaattgctct gtgctctgcc tatcaagcca agatgattgc tacggaaacc ggtagtgtct
    20221 ctgtcggtta cacaaaagaa aattttactg aagaagctta tcagatcgcc agaaattcta
    20281 tcattgaatt agtcgctcat gcagaaaatt ttggcgtaac tgttgctgtt gaagcaggga
    20341 tcaatcatcc aatctataac tatcagttag ctaaacgttt aatcgatgaa gtagcttcac
    20401 caaatttaaa aatcatttta gattgtgcga atttgatgca taaagaaaat catgctgatc
    20461 aggaaaaagt gatccgaggt gccttggaca atttacatgg ttatattaca gcgcttcatc
    20521 tcaaagacta tatcatggaa aatggaaaga tccgcatggt acctgttggc aagggctgtt
    20581 tagatttccg cccggtactg cattatatca aatatgagcg tcctctaatg tacgccactc
    20641 ttgaagctac tcctgagatt tatgttcctg aagcaataga acacttgcag tttatttacc
    20701 aagattgtta aggagaagat tatggaggaa cgaatacaga aaagttatca aaaatatcat
    20761 gcaaatgctt ttcagacatt gctgcatctt tatcagggaa attattggcg ttttgccatg
    20821 tcaggactct tttttattat caaacatctt ccatcttggt taatgccgat cgctattgcc
    20881 aatgtaatca atgcagcttc atcgaaagat tcttctcagt tgcaggccat ttatttcaac
    20941 gcttttttga tggcttgtct gattggtcaa aatatattga cgaattattt tcatgtaaaa
    21001 taccatagca cttcgatccg tcaagtagaa gctggactaa gagcgaccct tatcaagaaa
    21061 atccaagaac tatctattcc ttatcaaaaa gaattgcaat ctggacgaat ccagtcaaag
    21121 atcatccgtg atgtagaagc tatccaaacg ttgtcctctc aagtatttgt cagcggatta
    21181 aatatcgctg tcaatttatt cgtagcttta ggtatcacgc tttataaaag tcctgttgtt
    21241 ttcatgtttt tcttattgac tgttcctctc gccgcattcg ttactctctt tttccgtaaa
    21301 aggatcagac agtccaactc agacttcagg caagaaatgg aagagacttc tgcaaaagtg
    21361 atcgaagtga tcgaattagc accgattgct cgagctcatg ggttagaaaa acacgaaaca
    21421 actcgcttaa ataagcattt tactcaaatc tatgaaaaag gattccactt agatatcctg
    21481 caatctgttt tcggatcagt cagctgggct agttttcaga tatttcagct gatttgtttg
    21541 attttcactg ggatattagc tttacaaggg cgcatccaac ccggcgatgt cgtgatgtat
    21601 cagacgtatt tcacgacgat cgtcaattcc gtttctggaa tgatcacgct gattcctacg
    21661 atttcaaaag gcatggaatc tatcacttct attggcgaac tgttgattgc tggcgatgta
    21721 gaaacttatc acggtaagaa aaaaatccaa catttggatg gaaattttcg ttttgaggat
    21781 gtttcttttc tttatccgga ctcactcaaa ccgattatcg atcatttttc tctagaggtg
    21841 aaagctggag aaacaattgc ttttgttggg ccatctggct ctggaaaatc tacactcctt
    21901 aacttattga ttggatttat ccagccgact tcaggaaaaa tttttctaga tgatcagcct
    21961 ttggatgaac tggatatgcg aagtgtgcga aattatttag ccgttgttcc tcaaaccact
    22021 ttgttgttct ccgcttcgat caaagagaac atcacttatg gcttgaaaaa cgtatcaaaa
    22081 gaacgtttag atgaagtcat cgaagctgcg caactttctt cactgattga ccagcttcct
    22141 gatggtctgg ataccttagt aggcgaacat ggcaacaagc tgtctggtgg ccaaaagcaa
    22201 cgtatctcca ttgcacgagc gttgatacgt cagccaaaaa tcattttgct agatgaagca
    22261 acctcagctt tagataacca gtcagagaaa aaaatccagc aagccctgaa ttacctgaca
    22321 gaaacaccca ctacttttat tgtggctcat cggctttcaa cgatcaaaga ggcagataaa
    22381 atcgttgtga tcgatgaagg aaaaatcgtt gaaataggta cttacgaaaa actgttagct
    22441 caaaaaggct atttctaccg cttgaaaaat gaatgacaaa aaataggagc tatgacaaga
    22501 cttttgtcgt agctcctatt tctgaataaa cggtgttctc ctgccaactc ctcggtcaca
    22561 agtcacaata gtcatcaaac atgagaagct gttttttgaa attgttcctt gtaactcaga
    22621 gttaaacggt aggctcacga cctcttggta aatggtgttc aagagacagc ctatcggcaa
    22681 taagaagcaa ttttgtcatc gctcaaggcc gaacgtctct ttcacaccct ctgttgtttc
    22741 ataaaatagt tttattcgct gtaaaggtcg cgaactggtg tcatgatgat acggttcaag
    22801 tcattgatga ttgtactgaa gccttgctct ttttgcatca agtcattgat tacttcttct
    22861 gattggattt tcacagccat ttcttgtgct ttcattgcat cttcgtcgct gaactcttgt
    22921 ccactcattt gtttttcttg taattcgatt tgcatcgctt ggaagtcttt gaacagtttg
    22981 tgtgcttctt cgtttgcttg tactttctcg tacgcttctt tcaacgcttt gaattctggt
    23041 aattcacgga tctcacgttc gatttgattc gcgctatcgt agatgtttga catgttatgc
    23101 cctcttttct tttagtagtt actctctatt gtaacatggg gttattcgtt ttcaaagggg
    23161 ctactctcct tgatatttcc gccagaggtc tttagccgct tcaccaattc cctgaacgcc
    23221 ttctttcacc atatcgccga agtttttcag cccttctttt gcttgttcac catattgatt
    23281 caagttatct tgccaatttc cagactctcc attagaattt cctgtgtttc caacttgatc
    23341 tgcagctacc acttgtcctc ctgtggcata ggcatctgct acactaaatc ctgtttttga
    23401 agtatacgga aggatgcctt ctgccgctga tttgaagact ttcccgacaa catttccact
    23461 tgtcccttcc aaatagtgca gcttgctgga ttcttcgtaa cccatccatg tcgaaatcac
    23521 tacgtctggc gtgtaaccga tcatccattg gtcatttgct tttgtggcat cgaaattagt
    23581 ttctgtggtt cctgttttcc ctgctatcgt ataacctgca ggattcgcag cagccgctgt
    23641 tccatttgaa aatgtaccaa gaagcatgct ggtcatttca tcggctgttt cctttgatat
    23701 gacctgtttt ggttttttgt ttgtgttatc gacgatcacc gctccagtcg aatcaacgat
    23761 tttcgtgata agatgcggcg tatacatctg accatcattg gcaaatgcac tgtaagcacc
    23821 cgccatgatc aatggagata ctcctttttc cagtccacca agggctaagc cataatactt
    23881 atcagatttt gctaaaggca ggccgaattc ttctgcttta tcatatcctt tttgtaaacc
    23941 gatctcatgc aaaagccata cagtaggcaa attcaaactt tgtgcaacag cttgatacat
    24001 cggtacttct ccttggtatg tcccgtcaaa attatgcgca tcgtaataac tttgcggttc
    24061 gtctttcaag atactggaag gcttgtatcc agcttccagt gctggtgtat atacgcttaa
    24121 tggtttgatc gttgatcctg gtgaacgacg catctgtgta gcaaagctga atcctctaaa
    24181 gacatgatct cctcttcgtc ctactagtgc ttgtacacca cctgtattcg gatctaaagc
    24241 tactgaagca gattggacca tggcaccatc tgaagcattt gctgggaata atgcactgtt
    24301 ttgataagta gcttctaatt gcttttgata atcctgatct aaagctgtat agattttata
    24361 gcctttattc atgacatctt cttcatcaag accatactcg ttaactgctt catcgatcac
    24421 tgcatcaaaa taataaggat aacgataacc agaatctgaa ttatcgtatg tgtcattaag
    24481 atacgcgctg atatctgtct ggctttctgc ttgttcttgt tcttttgtga tttttccgtt
    24541 ttcttccatt acacttagta ctgtgttttt ccgatcattc gcattttttg ggtgatcgat
    24601 cggattataa ataccaggac ctttcagcat tcctgccaaa gtagcagcct cagacagcgt
    24661 aacattcgtt gcatctactc caaaatactt tctggaagcg tcttggacac cccatacccc
    24721 gttgccaaaa taggcattgt taagatacat ggtcaagatt tcttctttgc tgtacttctt
    24781 ttcgatctcg attgccaaaa ataattcctt cgcttttcga tcaaacgttt ggtctaatgt
    24841 cagatacgca tttttggcaa gctgctgagt gatcgtactt cctccgcccc ctgatgttcc
    24901 gccagacaag accattcgga ctgctgcacg ggcaatccct ttgatatcaa acccgtgatg
    24961 ctcgtaaaaa ttcctatctt ctgtcgagat tactgcatct tggatatagg gagaaatcgc
    25021 gtttaattct acatatgttc ctttttgggc gtacagtgtg ccagcttctt cgtttttctt
    25081 atcataaatg actgtcgatt ggcttaatcc tgattttaac gtttcgacat ttgcttgttt
    25141 tgctaaaata aagaggtaga tacttgtcac taagacacag atcaaaccaa tcagcaaaat
    25201 caatttgttc acatgatatt ttttccagat gcgctttcgc catcgatgga attgtccgag
    25261 atatggtctg atccaacgcc aaaaactgat caaagcagct tttatctttt ctaaaatgct
    25321 ttgaaaatcc atttgcttct tctcctgtct atttgattca atcgtcttat tttaccatag
    25381 tgaaagagtg ttttcttcct gcttaagttg tagttcaata tgttttttta cgttagtgca
    25441 agtagagtaa cataagaatg aacagaaaaa tgtaagtttt ttcttaaata tgttttaaaa
    25501 atggtacaat tagtctgcga ttgcactgaa aggagtacac ttgtgaaaat cacgatcact
    25561 ttacctatga accatcctga aacaacaatt cgcgacttgc ttgaaaagga atggttagtt
    25621 cctagaaagg ttcgacactt tttgcgtata cgtaaaaacg tttggatcaa tcaacaacct
    25681 gctttgttcc atcatgaagt aaagggcaat gaccagatca cattgttatt cgaagaaaca
    25741 gattataatt atcaagaagt tcaattagga gatgcgaagc acatccgtgt tttatatgaa
    25801 gatgagcatt tgattatagt ggataagcct gcaggaatga agactcatcc aaatgaaccg
    25861 acagaaaaca gtaccttact caatcattta gctgcttatc tcgacactaa acagcaacga
    25921 ccttacgttg tccaccgctt ggacaaagaa acaagcggtg ccgttttatt tgcaaaaaat
    25981 ccatttgtct tgccgatact tggaagaatg ttggaacaaa aaatgatcta tcggagatat
    26041 caagccatcg tatggggaga aattgccaaa gatatgacca tcaccaataa aattggacga
    26101 gatcgccatg atcggagaaa aagggtcgta gatgataaaa atggtcagag cgccttgaca
    26161 catgtcactg tcaacaaagt actttctgaa caaacacaag tctattgtgt tttggaaacc
    26221 ggacgtacac accagatcag agttcattta gctcataagg gacatccgat cgttggagac
    26281 cctctctacc aaaatagaac agctgaacga ctgatgctcc atgcattaga actgcacatg
    26341 atccatccat ttactcaaga aaaaatagtc ggtaaagctt tacctggatt atgggaatag
    26401 aaataaaaaa gaacagagtc ttatcagcag ataatgataa gactctgttc tttttgtgta
    26461 atttgtctgc ttacttttga tgttcgtgtt ctgctaacag caaactaccg ataatcccac
    26521 tttcattcgg cagtccccaa cgaacgatat attcacttac ttgaggtgtt tccatataac
    26581 cacccatcaa ttcaacaaat tgctgacgga ttttttgaag gagatgatcc tgattcatta
    26641 ccccaccgcc taaaataatc ttttcaggtg ctaacgtcaa cgtatagttg atcagtgctt
    26701 gagcaatata ataagcctga atagcccaaa caggatgatc ttgcggcaga tcttgccctt
    26761 tgatccctgt gcgtgcctca atagacggtc ctgctgctaa tccttctaaa caatctttat
    26821 gataaggaca agtaccttca taatggtcat ctggatggcg tttcacccaa atatgtccca
    26881 tttcaggatg tgcgatccct gaaaaaattt cttcattgat cacgacacct gcaccgattc
    26941 ctgttcccac ggtaaggtaa atacaactat tttttccttg tgctgctcct tgcttcaatt
    27001 ccccatatgc agcagcattg acatccgttg tccaagcagc aggaatattg tagcgttcct
    27061 tgattgtgcc aagaaaatcg aattgtgccc accctttttt gggagttgcc aaaacgtatc
    27121 cgtaattatc ttttgttttg tcgataccaa tcgggccgaa tgagccaatc ccgattccct
    27181 tcaattcata acgatcaaaa aattcaaata cctgatccaa tgtttcttct ggtgttgtgg
    27241 tagggatact taatttttct aaaatttctc caccatctga aaccgcacac acaaattttg
    27301 ttccgcctgc ttctattccg ccatacattc gttttcctcc tcgaatccga ttgtccgaat
    27361 ctcataaggt tggattattg ggacaaccgc ttgcttcgtt ggttcttcaa gcagattcaa
    27421 tatgacagtt tttccattgt ctttagttat ttccaatttt tctaaatgac tagacatatt
    27481 gaaacctctt actactactt tatcactaaa cttgctgcgt ttcaaagctg taattgcaaa
    27541 cgtttcactt tttatcgtta aatattgttg ttttgaaatc aatgttcctt catgatgttt
    27601 gatttgttga gtggaaaaag gaatctgtgc tgcgtatgca tgcaaataag tagagaattt
    27661 ttcttctggt ccatgtagtt caatgctgta attgaaccga tgctcaccta gacactgtgc
    27721 ttctggtgtt ggaaaatatc cccaatcgcc tagttctccc acactgcgaa gtagcgtcac
    27781 tgcgatttgt ccgtcttctg ttacttcata ttcattcaaa ccaaagtttc cgactgttac
    27841 accgtactct tcatcatgaa tattgacaaa tgcttgctga tgttgcggat tcgtggggtt
    27901 ttcccaactt ttagacacat ggtttgggcg cttcaccact tcaaaaatac tatctgcttc
    27961 gtgatgttcc acatgtaact ttgtaggaaa caagacacgt aagcgatgat ctttcatctg
    28021 attgtcgaga gtcgtttcaa agtcgatttt tttactatct tttctgattg tcatgattgt
    28081 ttttatttga aggattcgtt tttctttgga tcgttctgct tttcgataac gaaattccac
    28141 aaccatctgc tgttcttttt ctaaacgctc gtctgcagaa acagggattt ccatttcctg
    28201 tagaatactt atttttgcta tttccggtgt atctactaat acttttactt cagagttttc
    28261 tttgttactg gataggattg gctgatcaca gaaaggttgt ttaaagatat actcatttcc
    28321 tatatcgccg atatcttcaa aaatatgcag attattcaac tttcgtttgt ttttgcgatc
    28381 ttccattgta atggtaccat ttttttcaat cgtcaaatgt agagggccgt tttcgatgat
    28441 tcgtccactc tgatgaatca ttgtttcctt ttcttctgct ttcgcttccc cttctacaag
    28501 cgcgaatgtt tcccaagaaa atgctggcat gttttctaat ggtaaagtca ctttgatcat
    28561 tcttgccata tatggcacac ggaaacggtc tttcggcaaa tcataaccaa attggacagt
    28621 ttcttcagat aaaatagctg gtatagtggc cccacttttt gtttctacat gatacttacg
    28681 tttaggcaaa ttttctaact cttgatatag ttgttcaggt attccttcag cgaatctttt
    28741 tctttctaat tcgatggtta tttctgcttc accagtttta tccattcctg cagtatttac
    28801 aatgacaaat ggaaagctgt cttttgggaa tccggtcgta tcgattgctg cggtcagctg
    28861 ttctaaggaa tcttgtgcaa gatactttcc aacttcatct gctttttcaa aacgaggtat
    28921 catttcccga tggacagaat cgactgaaca accgcaaata ctgtcgtgag gatgattttg
    28981 cattaatgtc ttccaagcat aatctaattg atcatgggga tagtttccac taacttcata
    29041 agccatcgtc gcaagtggtt ctgtaatatt ttctagctgt cgctggacct tggtattcca
    29101 ttgttttaag taaacccgag cagatgctgt attggctaaa gtataccagc catccgtttc
    29161 ttgacttgtc aattctcctt ctactgagcc taagtcttct ggcacatctt tttgaactgc
    29221 ttccagatag tcagtaaaat tagaatgaat aaactcgtaa tctggaaaca gttcatttgc
    29281 gagatggatt gcttttgata tatctttttg taccggttga tgatcaacac cattcatcat
    29341 caataagtgg ttcgtagagg catattgttc tgcatccgct aatttttgct tccaaaaggc
    29401 aagagctgct tctttttcta ctggaatctc attcccgttg ctataccaat tggcaaatag
    29461 cagaccaaag attgctgttt gatcaggtcc tttccaccac atctctgagt attgggaaga
    29521 atagttttct gcttccaaaa cttgattatc aaagccaatt ggttttacac cacggccaaa
    29581 cgctgctgct gaaataccag cttgtttcat taattgaggc gtctgtccca tatttccaaa
    29641 tgtatcggga aaatagccta acatgacagg ggttccccat ttacggcttt cttccatgcc
    29701 aatcagcata tttctcacgt tcgattccga gctgataaga aaatcatctt gcaagatata
    29761 aaatggtcca attctcaatt ttccttcatt gattgcttgt tggattgcct gtcttttttc
    29821 tggccttact tgaagatagt catctaaaat aatcgtttgc ccatccaaat gaaaactgtt
    29881 gaaggacgga tcaacttcaa atagctctag tagatcgtct atcaattcaa tcagtcgcat
    29941 gtgatgctgt tcgtaagcca tgtaccattc acgatcccag tggctatgag aaatgatata
    30001 tactttcttt ttcatttacg atacctccta cttttctact ctaatatcaa agtaatccat
    30061 gactaattcg caaaacatca tatttgccca agaaaaccat tctctcgtat aattttcagg
    30121 attgttcaca tcgaatcctt cgtgcataag gtgtgttcct gcgtcagttg ctacaagtaa
    30181 attcaatatc cgctcttttt cagatttgtc tgacgttgtc attccttcca tagctaatgc
    30241 aatcggccaa acgtattttt ctggcgtatg ggaggatcca attccttttg cgtactttcc
    30301 ttcatagaaa tatggatttt ctttactcaa caatgttctt cttgtttgca gataatcttc
    30361 ctcttgttca gaacaatatc ccaagtaagg tgcggcgatc agattgggca cattactgtc
    30421 atccatgact gtggcgtttc ccattccatc tacttcatat gcataaatcg tttgcccttt
    30481 ttgattaacc gttcgtccgt gattctgaat cccctcttgg atctctttct tcaatttctg
    30541 tgtttgctgt acaatttctt tatcatcgag tatcgtttga aaaatttctt ccacataacc
    30601 taacacgacc accgcaaaca tattggatgg taccagatat ccataacggc aagtatcatc
    30661 gctaggacgg aaaccagacc atgtcattcc tgtaaaagct gtttttgcac ctcgcccgtc
    30721 attgatcagc gtatcttctt ttcttgtcgt gtctcgtaca aatgtataag gagagtgttc
    30781 atgatcttgc tctgttttga acacgtccaa gatttttttg ataccttcaa taaaagattg
    30841 attgaattga tcagtttttc ctgtattttt gtaaagaaga taggccaact gtattgggta
    30901 gcataaagaa tcaatttcat attttctttc ccagatccaa tcgttcattt ttgtatgatc
    30961 tgtttggtgg ccagcaccgt tagcttcttc gttgaaggca ttcgcatatg gatcgatatt
    31021 gatataaaaa aattgctttt tgactaaccc gctgatcatg tcagctaatt cctcatcttc
    31081 ttttgcaatc actaaataag gacgaacttg tgcagtagag tctcttaacc acatcgctgg
    31141 aatgtctccc gtcaataaaa aggtggttcc gtcattttgc tttttgactg ttgtcaacag
    31201 agtattggca aatgctgctg taaaattctt tgcccagtcc gcatggatat ccccacattt
    31261 ttgtgtgata tcttccataa atttctgcac cgattttggt atatctttat aagccactaa
    31321 taaaaccttc tttctatttg atatatcttt aaagattata cttcatacag ccaattatgt
    31381 ctacgctttc aaaaaatatc tttttattta ctttttcatc cattatggta ttatttgaat
    31441 atgatatatc attaagaggt gtttatatga atcaaccatt gtaccagaca atatttaatg
    31501 atttgcaaac ggctatttta actggtgaat tacctgtaga cagccaacta ccaactgaaa
    31561 aagaactttc acgtacctat aaagtaagca gaatcacttc caaacgtgca ttgactgaat
    31621 tagagcaatt aggacttatt tatcgcgttc gcgggaaagg cagtttcgtt aaatccgctt
    31681 ctccggaaca gattcttgct tctcctacaa aaacacgacg gattctgttt cttcttccat
    31741 atgtcgctga tttgtctgtt ggtgatttta gcaagggatt gaaccctgtg atgcaagata
    31801 aacagattga tgtcatgatg acgacgcttg actttctaca aaataaaaca gcaaaagagc
    31861 taatcaatga atttgacgga ttgatttatt atgctcttga tacagaacaa catcttgact
    31921 tactttttga gctttcattg aaagaattcc ctgtcgtcat attagataaa aaaatctatg
    31981 agttaccttt tcctaccgtt ctttccgata atttccaagg cggtgcgctt gctgcctctt
    32041 atttgatcga acagggacat tcaaaaatcg cttatttgtt tggtacgcag actcatccac
    32101 aatccgttcg acagcgttac cttggttacc ttgaagcatt ggacaaagct ggtttgactt
    32161 tccacacttc actggatgac aaaagcgcga cattagatga attgctttct tatatcacta
    32221 agtatgaagt aactgctttt gtttgtgaaa acgatgttgt cgctatccag accatgaatc
    32281 tcttgagaca gcatggttat catatccctg aagatttttc aattatcgga tttgatgata
    32341 tccaagcagc agctcttgtt gacccgccac tgacaaccat cgcacaagat tttgaacagt
    32401 taggtaagct tgcgggagat gctttggtca cttggttaga gacaaaagat cgaccatccg
    32461 atagtaaatt tccagtttca ttcatcaaaa gacaatcaac aaaggagaaa taaaatgaat
    32521 atcggtcaaa tagatactag acatgcaaca gcgaatcagc caaatttctc taatgggaac
    32581 tgccagcctt atactggcgt tccttttggg atgaattatt ttgcaccgca aactacggat
    32641 caaaacggca gctggtggtt tcatcctgat gaccgcgttt ttcaaggcta ccgtcttacc
    32701 catcaaccaa gtccttggat gggtgatttc agtcatatgc tgtttacgcc gatcaatgga
    32761 aaactacagg agaacaccct tttccatgct caaagttctt accgcccaga agaaagtata
    32821 ttttgcccga ctcatctgtc gatctgtcaa ttacgttatg gcatctgttc tacattgata
    32881 cctagcatgt atggcggaat cttatctatt gattacagca aaaatgacag cggtttactt
    32941 ttatcctttc cgggacgcca tcagctcttt gttatagatc cttacacagt ggaaggaaaa
    33001 atcacacatt ttgctggttc agaagatcct gatttttctt tttattttat attgagattt
    33061 gaatatcctt taacaattga tgagttagct gtttccaaca aaaatagcga ctcgatcccg
    33121 ttgtattttg aacagacaaa aaaacaaact atccgatttg ggacatcttt tatcagctcg
    33181 gaacaagcag cttataacct ggaactagaa caaaaccaaa gcaaagagac ttaccttgct
    33241 cacagtcaag aacaatggca gtcctatttt gatcgtatac agctcacaca ccatgatcaa
    33301 caacaattgc ggacgtttta tcataattta tatcgggtgt ttttatttcc acaaacattt
    33361 tatgaaattg atcctagtgg gcaaaaaatc cactatgata cgactagtcg atctataaaa
    33421 cctggtgttc tctatacaaa taatgggttt tgggatacgt ataaaacggt ttatcctctt
    33481 tattctttga ttgctgtaga aaaatacgaa gaaatgctgg aaggtttttt gaacagctat
    33541 agagaaagtg gctatttgcc aaaatggctg tctcctgatg aacgtggact tatgcctggg
    33601 acattgatcg atgcggtcat tgcagatgct gctgtaaaag atatccgacg agacttgatg
    33661 cctgaatttc taacggcaat ggaaaaaggt gctacggttc aaagcaaaca tccaaattat
    33721 ggacgccaag gaacagaaga ttacttgaca tatggctatg ttcctaatca ctaccacgaa
    33781 tcagtcaatc acacattaga ctatgcttat agcgattttt gtattagcca agttgccaag
    33841 gtactagaag aagaggaaat gacgaagaaa tatcagcagc aatcattgaa ctatcaaaat
    33901 atctttgact atgagtcagg atttatgcgt tcaaaagata aagatggcaa gttcagagaa
    33961 ccattttcag ctaatcgttg gggacaagat tatgcagaag gaagtgcttg gcaaagcagc
    34021 tttgctgttt atcacgattt ttcaggactg atcggaaaat atggtggcag tgagagatat
    34081 aaagaaaaac tcattgagct gtgcaatcag cccccaacgt ttgacccagg aggatatggt
    34141 tttgagatcc acgaaatgag tgagatggcc gcgatagaat ttggacaatt agctatttca
    34201 aaccagccaa gttttcacta tccttacttg ttcagttata taggacaacc tgaaatggca
    34261 cagccactgc ttaaacagtt gatgactcag accttcgacg attctccaat gggttaccct
    34321 ggagatgagg acaatggaag tatggccgct tggtatatct tcaatagtct gggattttat
    34381 cctgtcacgc ctggaaccgg tgaatatgtt atcggtatgc cattaattga caaggctgtt
    34441 ttgcttttgt caaatggaaa ggaattgacg attgaagcaa cgcctaatcg gccgcagcaa
    34501 caatttatcc ataaaatcaa acggatggac aaagaatata cgaaattata ttttacccat
    34561 caagatttgc tgaaaggcgg cacgatctct taccaattag ggatcatccc tcaccctaag
    34621 aaatatcaag caaaagattt gcctttttcc ttatcaaact ataattaaca agaggttgtg
    34681 aaaaagctgt cctgcatcaa gtaataagaa cagccaagca aaaatagatc ctcatatttt
    34741 tcgtttggtt ggtcttatta ccgcagatgc aagcttttga acaccgttta ttgagaggtt
    34801 gtgaaatcag cgttctgacc tgagtattag agagacaaat ggcaaaatag ttcctcatat
    34861 tttgccattt tttgagctaa taccgagggt cagctgattg aacaccgttt agtggagagg
    34921 tcgtgaaaaa gctgtttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac
    34981 attttt
//
LOCUS       DAYYAO010000022        34760 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000022 DAYYAO010000000
VERSION     DAYYAO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.023"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(471..2363)
                     /locus_tag="ACHARI_000978"
     CDS             complement(471..2363)
                     /locus_tag="ACHARI_000978"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442042.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2360..4087)
                     /locus_tag="ACHARI_000979"
     CDS             complement(2360..4087)
                     /locus_tag="ACHARI_000979"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442043.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4103..4753)
                     /locus_tag="ACHARI_000980"
     CDS             complement(4103..4753)
                     /locus_tag="ACHARI_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8442044.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4957..5253
                     /locus_tag="ACHARI_000981"
     CDS             4957..5253
                     /locus_tag="ACHARI_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8442045.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5445..6065
                     /locus_tag="ACHARI_000982"
     CDS             5445..6065
                     /locus_tag="ACHARI_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8442046.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6157..7323)
                     /locus_tag="ACHARI_000983"
     CDS             complement(6157..7323)
                     /locus_tag="ACHARI_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8442047.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7335..7688)
                     /locus_tag="ACHARI_000984"
     CDS             complement(7335..7688)
                     /locus_tag="ACHARI_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8442048.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7816..8838
                     /locus_tag="ACHARI_000985"
     CDS             7816..8838
                     /locus_tag="ACHARI_000985"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8442049.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8857..9018
                     /locus_tag="ACHARI_000986"
     CDS             8857..9018
                     /locus_tag="ACHARI_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442050.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9277..9711)
                     /locus_tag="ACHARI_000987"
     CDS             complement(9277..9711)
                     /locus_tag="ACHARI_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442051.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9821..11680)
                     /locus_tag="ACHARI_000988"
     CDS             complement(9821..11680)
                     /locus_tag="ACHARI_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8442052.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11681..11821
                     /locus_tag="ACHARI_000989"
     CDS             11681..11821
                     /locus_tag="ACHARI_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8442053.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11798..12958)
                     /locus_tag="ACHARI_000990"
     CDS             complement(11798..12958)
                     /locus_tag="ACHARI_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8442054.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13265..14689)
                     /gene="araA"
                     /locus_tag="ACHARI_000991"
     CDS             complement(13265..14689)
                     /gene="araA"
                     /locus_tag="ACHARI_000991"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8442055.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14705..15403)
                     /locus_tag="ACHARI_000992"
     CDS             complement(14705..15403)
                     /locus_tag="ACHARI_000992"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8442056.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15360..17021)
                     /locus_tag="ACHARI_000993"
     CDS             complement(15360..17021)
                     /locus_tag="ACHARI_000993"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8442057.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17239..18639
                     /locus_tag="ACHARI_000994"
     CDS             17239..18639
                     /locus_tag="ACHARI_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8442058.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18891..19970)
                     /locus_tag="ACHARI_000995"
     CDS             complement(18891..19970)
                     /locus_tag="ACHARI_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8442059.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20056..21015)
                     /locus_tag="ACHARI_000996"
     CDS             complement(20056..21015)
                     /locus_tag="ACHARI_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8442060.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21027..22538)
                     /locus_tag="ACHARI_000997"
     CDS             complement(21027..22538)
                     /locus_tag="ACHARI_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8442061.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22560..23124)
                     /locus_tag="ACHARI_000998"
                     /pseudo
     CDS             complement(22560..23124)
                     /locus_tag="ACHARI_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(23130..23993)
                     /locus_tag="ACHARI_000999"
     CDS             complement(23130..23993)
                     /locus_tag="ACHARI_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8442062.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24007..24903)
                     /locus_tag="ACHARI_001000"
     CDS             complement(24007..24903)
                     /locus_tag="ACHARI_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8442063.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25078..26367)
                     /locus_tag="ACHARI_001001"
     CDS             complement(25078..26367)
                     /locus_tag="ACHARI_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8442064.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26339..27856)
                     /locus_tag="ACHARI_001002"
     CDS             complement(26339..27856)
                     /locus_tag="ACHARI_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8442065.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28054..28944
                     /locus_tag="ACHARI_001003"
     CDS             28054..28944
                     /locus_tag="ACHARI_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8442066.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(29017..29841)
                     /gene="nadE"
                     /locus_tag="ACHARI_001004"
     CDS             complement(29017..29841)
                     /gene="nadE"
                     /locus_tag="ACHARI_001004"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8442067.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29853..31325)
                     /locus_tag="ACHARI_001005"
     CDS             complement(29853..31325)
                     /locus_tag="ACHARI_001005"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8442068.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31962..32324)
                     /locus_tag="ACHARI_001006"
     CDS             complement(31962..32324)
                     /locus_tag="ACHARI_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8442069.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32547..33299
                     /locus_tag="ACHARI_001007"
     CDS             32547..33299
                     /locus_tag="ACHARI_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8442070.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33439..34362)
                     /locus_tag="ACHARI_001008"
     CDS             complement(33439..34362)
                     /locus_tag="ACHARI_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8442071.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34377..34649)
                     /locus_tag="ACHARI_001009"
     CDS             complement(34377..34649)
                     /locus_tag="ACHARI_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442072.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10258 a         7232 c         6007 g        11263 t
ORIGIN      
        1 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa aaagtcgctt
       61 agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg cataaatttg
      121 gcttaatacc gagaaactgg cttttgaaca ccgtttacca agaggttgtg agcctgccgt
      181 ttaactccga gtcacaagga gcaatttcaa aaaacagctt ctcaggtttg ttgactattg
      241 tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa ggaagaggtt gtaaaatcag
      301 cggtttgacc tgagtattag agatacaaaa cggaaaaata gcttctcata ttttgccgtt
      361 ttttgagcta ataccgaagg acagctgatt gaacaccgtt tattcggaaa aagggactgt
      421 gacacgactt ttgtcacagc ccctaaaaaa aataataaat ttgtactatt ctatgaagct
      481 tcttcttcag aaaactgact gttataaaga tctgcataga atccgtcttt ttccatcaat
      541 gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca tcacaataat gttgtctgca
      601 tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg tccggttttc caataggcgg
      661 ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta cactagatgt tgcttcatca
      721 aggatcagga catctggatt tgctaagaat gcccgcgcaa ttgtgattag ctgacgctgc
      781 ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt tatatccttc tggcaattta
      841 cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa tgatttgttc gtctgttgca
      901 tcgtcgttcc catatttgat attgtcatag atacttcctg taaacagcca agtatcttgc
      961 agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa gatcacgggt atctacacca
     1021 tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt caagaaggtt gatcagtgtc
     1081 gtcttccctg caccagtcgg acctacaata gcgaccatct caccaggttt gacatccaag
     1141 ttaaaatcag tcattagcaa gttatctggt gtgtagccga attgaacatt ttcaaaacga
     1201 actttgtatg gtgtatcttt ttcttctgga aggccagatt tttcattgga catctcttct
     1261 tcatccaaca cttcaaatac ccgttcggct gaagcaactg ttgattggat agtgttcatc
     1321 aaacttgcaa gttgagtgat tggttgagag aactgtgttg tatattgcaa gaacgcttga
     1381 acatccccta atgtcatgtt cccatttgca acttttactc cgcctagtac agcgacgaac
     1441 acatagccta gatttttaac gaaattcatc aacggcatga tgattgccga aataaattga
     1501 gctttccaac cagcggcata cagattctga ttttcttttt caaatacttc ctggtctttt
     1561 tcagtatgat tgaatgtttt aacaatggta tgaccaccat atgtttcttc tacttggtta
     1621 ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca cttgtgattt cggcgctacg
     1681 atcatcacga tgatcaagct taaagggatc atcaataacg cgactaacgt caattgccag
     1741 ctgattgaaa gcatcatcac gatgacccca atcaatgtga ctgcactagt tacaaattga
     1801 gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat cattgattgc tcgagacatg
     1861 atatccccat ttgtatgtgt atcataatag gaaatcggta cacgattcat tttggcttcc
     1921 aaatcatgac gtaattcata aactgttcgt tgggaaacac gtgtcataat aaattgctgt
     1981 aaaaagttga atacagcaga aatcaaatac atcacgatta ctgtcgcaat gatctgcgcg
     2041 attttatcaa agtctatcgg aaattttgtt acttgttggc cttgttgcat ctgttgagcg
     2101 ccagccatta cacctttgaa tatttctgtg gttgctttcc ccaagacctt tggtgtttgg
     2161 atttggaaga tcacggctgc tattgctagt acaaatacag cgatgatcgc aaccatacgt
     2221 ttagacatat agccaaacaa tcggcgtacc gttccccaga aatttttagg cttttcacct
     2281 tttacaccga gatttctgcc aggtccacca tgtcctggac cgcctgctgg tccatgagag
     2341 ggacgtgtgt ttgttttact catgctaagt cctcctctct tagttgtgaa tgaacgattt
     2401 cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt tcctttgcca accagttttc
     2461 cttcatctag cacaaggatc atatctgcat ccattactgt acttactcgt tgagcaacta
     2521 aaacggtgat actttctttc atattcgttt tcaatgcttg gcgaagattg gcatccgttt
     2581 tgaaatccaa cgctgagaag gaatcatcaa acacatagat atctgctgtt ttgactaatg
     2641 cccgtgcaat tgccagacgc tgtctttgtc caccagagaa gtttcctcct ccttgttcca
     2701 catggctatc taagccatct tccatctctg aaacaaaatc tttagcttgt gcgatttcta
     2761 atgctttcca aatcatttca tctgttgcat ccggcttacc atactgcata ttgtctcgga
     2821 tcgttccaga gaacagaact gctttttgag gaacaaagcc cattaattct cgcaaattat
     2881 attgagtcat gtcactaatg tccgttccat ttatttggat cgaaccagat tcgatgtcat
     2941 ataatcgagg gatcaaattg actaaggtcg ttttacctga accagtcccc cctatgattg
     3001 cgacgatctc gcccgacttg gcttggaaat caatatcttc caatgcaagt ttttctgctc
     3061 catgatatcg ataattgaca tgattgaagg ccaatgttgc gttttttcca gcaaaagaaa
     3121 ctgttttagg attttcaggg tcatggatac cgattttttc atccaacact tcattaatac
     3181 ggtcagctga agcttgtgca cgaggcacca tgataaaaat agcggaaagc atcataaagc
     3241 tgatcaagat ctgcatcgca taagtcataa atgcaatcag attaccgact tccaaagtca
     3301 tatctgctat gtagtgtcca ccaaaccaag tgattgctac gtttgtaccg ctcatgatca
     3361 atgtcatcat tggcatcatc aatgcgacga tcgtattaac tttgattgcc gtatttgtat
     3421 aatctttatt cgctaaatca aaacgatttt cttcaaagcg tgtcttatcg aatgctcgaa
     3481 tcactctgac ccctgtcaat ccttctcgaa ataccaggtt taaacggtca gttttctttt
     3541 gcatactttt gaataatgga acagcaaagt acatgattcc gccgatcaaa ataatcatta
     3601 ctgggatgac aactaagaag atttttgtca actgatgatc tttttgataa gcaagtatgc
     3661 tcgcgccaat caacgtgatt ggtgcattga tcatcatacg taaaaacatt tgtgtaacca
     3721 tttggatttg agtcacatca ttggtcgttc ttgtgatcaa agaggccgtt ccatatttat
     3781 caaacgcatc ttttgtcaga ctctctgatt ttttatagac atccgtacgc aattgcttac
     3841 ccagtttctg tgattcacga gtagcaaaaa atgtatttgc gatagcagct aagatactga
     3901 tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat ataatcgaca tctccttgcg
     3961 ctaccccttt atcaataata ttggaggtaa gtgtcggcaa atataaatct gccagtactt
     4021 ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa ggagatccgt tttacgattt
     4081 ttagcatcta gttcccttct ttctatttat atactccgtt tttcagccaa gaaagtttca
     4141 aattgaattc actgattgct tgctctactg agtaatcttc agatgttgaa aataagcgat
     4201 acgaatgcgc aattgttgta aatacagtat tcatcactaa ttggatcagt aattccaatt
     4261 catgatcatt ttttactttc agcttcgtcc gatcgatcaa ttcaagcaac tcttttgcat
     4321 cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg gcgactcgta tgaggagcga
     4381 cttttcttga ggcacggtaa tccatgttca taaacaagtt acgataaaaa gaagcattct
     4441 cgccttccaa aatctcataa atcattttag aaaaatagga ttcgaatcct ttgaatagat
     4501 caccatcgga ttctttcatt agttgtaaca tatctttaga gctgtcacgt ctcattgtct
     4561 caaagtaata aaaataaaga tcctctttat cttcaaaata ctgatagaaa ctgcctctag
     4621 ggatatccgc taatttgacg atattagcaa tcgaagcttc ttttaagggt actctagaaa
     4681 actcgattct cgcagcctcg attagtcttt tttgtttttc tttaggcaaa tggaaaaatg
     4741 tctgtgtcgg catatggatt ctcctttctt tcaagatgac acattgtcat atgacgctat
     4801 gtcattataa cacctcctac ctgttttgca agacatttgc atagtttttt tcattatcat
     4861 ccgatacatt aaaaaagtga taagaggaat ccttccattt gaattaatga gagttgccca
     4921 ttaaaatagc cacatactaa acaaaggatg agataaatgg cccaatcaat tagtgtccaa
     4981 gacttcaata aattgcctct cgatgctgac agcatcgttt tagatacaag aaatacctta
     5041 gattacacaa aaaaccactt aagccactcg ctttctatcc ccgctgctat cttgccaaga
     5101 caattaaaaa atctagatcc aaagaaactt tattatattc taagctatac aggcaggcga
     5161 tcagaagcat tagccgatca acttactgct aatggttttc gtgctgttta tatcattggt
     5221 gggatggaag ctcttcagtc gagtgttgct tgaatattgc ttgttagatt caaaagaggc
     5281 tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt ctttttttag ttatttattc
     5341 ttcaacctta tcaatatgac ttgatagcct tttctttttc gagtaaactg attttgttag
     5401 tcatagttat aacaatcata gcaataaaag gagaaggtaa gcatatggca tctgttttag
     5461 tagtcaaagg tcatccccta actgcagaag aatcgcgcac agttaaggca ttgacgtctt
     5521 ttttaacaag ttataaagaa aatcatccag atgatgaagt gactgttttg gaactttatc
     5581 gcgatgatat tccggaaatc gatgaagaat tattatcagg ttgggaagca ttacgtgctg
     5641 gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa aattgctcgt ttcaatgaat
     5701 tgactgatca gttccttgct gctgataaaa tcgttatcgc aaacgcctta tggaatctaa
     5761 atattccaac aaaattaaaa gcttggttcg ataccgtcaa tgtagctgga aaaacgttcc
     5821 gctatacaga aaacgggcca gaaggattag tgacaggtaa aaaagcactg cacatccaat
     5881 ctaatggcgg tgtatacaac ggacaagact ttgcctctca atatgtaaaa ggtattttaa
     5941 actttgtagg catcgatcaa gtagatcagc tattcatcga aggaatcgat tatgaccctg
     6001 accgtgcaga tgagttgatg caaaatgcat tagataaagc tgctgcttta ggtaaatcat
     6061 tttaaaaatt gagataaatg catacaaaag agacagagga aaaaatcttc aaagcagcat
     6121 ttgtgccttg gatattttcc catctgtctc tttttgttat tctttctgct ttttcaacgg
     6181 attttcttca taaagagaag agattgttgt gtaccattca aaaacatgag taatcactac
     6241 tttagctgct gcataaacag gaatccccaa gatcacaccg atgattccga acaatttccc
     6301 agaagtcagc agaactaata aaatcgttac tggatggatt gctaactgat tccctagtac
     6361 taatggagag atcaagcgtc cttctattgt ctgctctact gcaaaaacga tcagtacttt
     6421 gatgatcatg aatggacctg ctacaatggc taaaaagata gccggaatca tcgctaaaaa
     6481 tgatcctaag taagggatca ggtttaaaaa acccgcaata atccccaacg tgacagcata
     6541 ctctaaacca atgaccgcaa aaccaatcat aaacatgatt gccacagcaa aagccacagt
     6601 tagttgtcct cttatataag aagaaacttg actattcatt tcactcagca cattcaacgt
     6661 cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa ggtgctaatt gtttaccgtc
     6721 tttcaataga taaaataaga taaacggcat cgtcagcaga gccactaaaa tagtcgctac
     6781 cgcgccgaca aaacttccaa gactctgaac tgtactctta gatatatctt ggatcatatc
     6841 gcctgccgaa ttcatgaact tatcacctgc tgtttccaac tgttcgcgaa attggttgaa
     6901 cagcgggtcc cgcaaaatct cggttaattt attatcaatc gtatctacat attgagggaa
     6961 gttactgatg aagctcactg tttgttcctg aatcttaggg atgatcacta cgctccccca
     7021 tgcgattagt gcaacaacca aaacaaacag cccaatgata ctgtaaagcc ttggtacttt
     7081 tcgcttttca aagtaatcaa ctaccggatt catcaagtaa tacaagattc ctgctaaaat
     7141 gataggcaga cctacgattg ctaaaaattg ccagattggt gcaaacaaat atgaaacttt
     7201 tgtaaataaa aagacgatca gcaatattaa caatacaaca agtaaggctg tgactacttg
     7261 attatttaaa aaccagcgcc aaaaccacga taccggtctg ttcttcttat tttttccttc
     7321 cataattcct cctgttagtt ataaaaaatt tggcttccca aaccaatagc aggcagtcta
     7381 tgcggatgaa gatagattcc atttgcaatt tgtccttctt ctggacaagc atcaaaaatc
     7441 aatgatacgt gagctactgt ctttaaattc ccattggcca tcggaccgac ataatcgatc
     7501 gtatatacct gatcatcaaa agcaattgtt ccgcctgctt tcaaagaaaa aggttcgtct
     7561 tttgatactt cttgaatcac cgaatatttt cgtaaggcag ctgttgctgt tttatcgaac
     7621 aaaatcagca tgggttcttc cttgtttatt gcttctgtcc caatttctgt aatctttgcc
     7681 tgaatcattg cactctctcc tttaattgct ttcaaaaaca gtataacaca ttctcattct
     7741 ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa aactatttca ttacaattag
     7801 gaggaatcag ctcatatgat cgaaaagata attttaggaa cctatacgcg tcgcgaaagt
     7861 aaaggaatct atacgatcga ccttgataca gaaaaagaag aattagccaa cctttcatta
     7921 gctgttgaag aaaatagccc tacctatgta gcaaaaagta aagctggcaa tctttacaca
     7981 gttactagtg tggatggatt aggtggtgcc ggtgcgtacg ataaggattt tcattttttg
     8041 aatgcggtaa cagagtctgg cgctcctttg tgttatgtag cggttgatga agcacgtcaa
     8101 ttactatacg gggcgaacta tcataaaggt gaagtaaatg tctaccatat attaggagat
     8161 ggcgaactga aagcagcaga ttcaatattc catcaagaag cgacaggtcc tcataaaaac
     8221 caagatcatg cgcatgtcca ctatacggat ctgactcctg accaacgatt agtcgtatgt
     8281 gatcttggta cagatcgtgt gtatacttat gatgtgtccg aaaacggcaa attgaatctt
     8341 gtaactacct tcactgcaga gccaggaaca ggccctcgcc atcttgtctt tcatccaaat
     8401 ggacaattcg cttatttatt tggtgaatta gacagcacag tccgcgtgct ttcttataat
     8461 aaagaaacag gtgcttttga agaattacag aaaatatcga ccttgccaga agaattcgac
     8521 ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg gcaagttcct ttatgcttct
     8581 aaccgaggac ataactcgat tgctgtattc gcagtttttg aagatggttc ccacattcaa
     8641 aacaagcaaa tcatctcaac agaaggggat tttccccgcg atttcgcact agatccaagc
     8701 aataattttg tcgtgtgtgc caatcagaac accgataatc tgacattata tcgccgtcat
     8761 cctgaaacag gattactgga attactgaaa aaagatgtct atgtaccaga atgtgtctgt
     8821 gtttattttg atcgttagta agaaaaggag aatgaaatga atcagcaaga cgtattagac
     8881 agactacggg aagaactaaa tattcctttc tttgatggga aaatcgaaga taaagagtat
     8941 agcgaagagg aatatcagaa actcaaagcc gatttacaga actactttga acaatatgta
     9001 cagaatgtag aaaactaatc agctttccat actatctgaa aagaacatca ggacaagact
     9061 tttccgtcta tcctgatgtt cttttctaca aataaaaaac cgccgagatg gttagactcg
     9121 acggaaaagg agtatttact cgattttgag taagtacaag gttgttgttg gttataggta
     9181 tgtcattatc ataaaggatt ttgatcactc tatcataaag gtaatattac atttatatta
     9241 gataactcta gtttttattg ttatcaaaga tttcttttat tttttcaaca acaaattagt
     9301 cattttttgg atggcagctg tcaattggtc tgtcgcttgg tcttgttttg cttcagatgt
     9361 attgaataga tcttcatcta cccctgattc aagatcgata ctttggatca tttcttcaca
     9421 gcctgctaca tatgaaagat aagctttttc aaaattttta tgaatgccca ttgcttttgc
     9481 tggtggacgc aaagtactga ctttcataag cattgcccga tatttttttg ttccttcttc
     9541 aaataacagg atgatctctt caagagtcga tttgtccagt tcacttgttt ttccttcatc
     9601 aattgcgttt ctgatcactt catatttcgg atgcattcct tctccgattg cttctgtttc
     9661 cttgacgatt tgatcgatgg actgcacata atagccaata ttcggtaaca tgttcgtttt
     9721 cctactttct ccattgttgt tattatttat tctacttgtt tttatcctca aatgacaagt
     9781 acttgaaaaa atttaatggt ttctatacag ctcgccattc ttattcgtgc tcaaagaaac
     9841 catctgctaa actatattgt ttccctgcga caatgtcata ttggatcaat tgatattcgt
     9901 cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt ataccactgt ccgttttgat
     9961 agtacagttc tcgttcaaac gccggaagtt cctgttctaa tgataaaagc agctgataaa
    10021 agccatttgt tgtctgattc gtctgttcca ttagattcgc tgcaaaataa aacggactgg
    10081 tgattgcatc gtgagtcgtt tgcttttcta attctccttt actgtcgaac ataagaaatt
    10141 gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat cgtatctgaa tagatccctg
    10201 gaagatgatc cccccaaaaa acaactaaag tccttctaga aagattcttc aattcttccg
    10261 tgaactgctt cagtgccgtg ctgctatagg aaatatcttg cagataattc tctaagtcaa
    10321 gtgtcccact tccatctgaa gtttttgcag gatagtctag attatcatac tttccatcgt
    10381 agggcatatg tgtctgcatc gtgactaaat gaatgaactg aggtgttttt tcttccttga
    10441 gaagtgtcat tacttctttg taagctgact catcagaaat atatggatta ttctctatcg
    10501 tatccgtata ggtcatcgta cgctcgctga tgaactggtc aaatcctaat gtctgataaa
    10561 catcctctct tttatacatc gaagtattgt atggatggat cgctgtcgtc tggtatgatt
    10621 gtgcgtttaa cgctgaaacg atagaaggaa gctgatccat ttttggtaca agcatcgtgt
    10681 aaggggtggt gagttgcgca ttaaataacg ccatagaaaa accagtcaat gcttcaaatt
    10741 cgatatttgc tgttccaccg ccatagtttt gagaaagcat attgccacta tatgtttgat
    10801 cagctacttc ataataatct gccaatggct caccagatac ttctacgccg tttaatcgac
    10861 taggatctga gaaactttca ctcatcacga acacgatgtt cggcgactct tcctctacag
    10921 ttttattttt ttcatcggct agcttctggt atttttcagt gatttctttg atcttttctt
    10981 tcgaatatcc ttcaggttca tccattggtt ctaccttcag attgtacaaa aacccaccga
    11041 taaaaccagt gttatagtag ttcattttct ggctgtaagg tatccatagt gccgttttat
    11101 tatatgcttt tcgcaacaaa ttatcgggat tgttgaaatt actgatatac cctagcagtc
    11161 caattgccac aagcagtgtc gttaatcgga tcaattggaa tcgccttcct tttttcaggc
    11221 ttcgaaacat gtaccagcaa aacaatccta tcacggttcc tgcggcagct aagatcactg
    11281 taaacagcat agtgccggtc atatctttca gcaatccgat ttcagtgatc atcttcaaat
    11341 cgtctggata aatcggttct tgccgataac tcattttcat ataatttgca aaacctaata
    11401 caccgatggc tacgacatac gtaagggaac ctaaagggac agatcctgct agtgcaatca
    11461 gaaaaataag aataatcagc aagacgagac aggctaagaa gaatttttcc gtatgccaag
    11521 aaaaagcaaa tttcaacgca aggtctacgg atagatcatt ttgacaccac tgcaaaaata
    11581 aattactaaa aacaacaaga aaaaagatac tgatgattgc tgctgttcct tttaaaatct
    11641 ttttctgttt ggtcgaaagt ctgcttatat atcttttcaa atgcttgctc cttcatttct
    11701 atcaataaaa gaatgttgct tctcttattt tagttgtacg aaattcgaaa gtaaagaacg
    11761 gaaagaagaa ttaatgaaat ccaaatattc tctttcttta tggcctttct ggtttcttta
    11821 ataagcggac ctttcgtact ccagaaattt tttctagctg ttgtttcact tgattgagct
    11881 gttcaaaatc agtactgtct atgtcaatca atatgtagac atgctcattc ttatggttac
    11941 gcgatatatc tgcgatcgct agctcgttct cattcagcag agaaaatatc tctgctaata
    12001 tattggtttt cttttgataa aatatagtga atcgaaacgg cgcttggaaa aacattcgtg
    12061 cggatggata attgaccgct tctctaaccg taccgaacaa aagataatct tttaaggctt
    12121 caactgccaa acgtccgctg tctgccaaag cttcctgagt cgatccccct agatgaggca
    12181 acataagtat acggtcattt tctaagaact cttcttctgg aaaatcagtc aagtatttag
    12241 ccagatgttg tttttccaat gcttgcataa cagcatattt atcaacaatt tcacttcttc
    12301 ctacattgat aagtacagca tcttttttca ttttttctat attcgcctga tcgatcaatc
    12361 cttttgtatc ttctgtcaac ggcagcatga ctacgatgta atccgaggta gaaagaactg
    12421 tttctaaatc tgcttgccga acataatcca actgggcatc gcggatagaa taacccaaga
    12481 catccatccc taagctatag caacttagcg ctacttttgt gcctatcgct ccaagaccaa
    12541 gaagaccgat ggttttgcct tgcaattctc gtcccacata agcacttctt ttgttttctg
    12601 cctgttctaa aatattagga cctgttagtg tttgtaccat acgacttgct tcgattatcg
    12661 gacgggaaga taagagcaaa cagcacaaaa caagctcttt tactgcatta gcatttacac
    12721 ctggtgtatt catgacgatc gtcccgtttt cagaagcttt ttcaacgttg atcgtgttca
    12781 ccccaacacc agctcttgaa attgctagca aatctggtgt aagccattca tctttcactt
    12841 tcgtactacg aataactatt ccttcggctg gcaaatcatc tattattttg aaatattccc
    12901 cttgtaactt ttctatctct tgttctggaa tagcatcaat caaatgaacg tgatacataa
    12961 aaccccctcc ttactaacaa gtatctcgaa atttctgcaa ttagacaact gtttaccatt
    13021 tgacttccta gaagcaaaaa gagcagttag agcagagtag gctgagtgat aaaaataagg
    13081 ctatgacaag ttactgccat aagccttatt ttcgagagca aaatattgag ggtgagatat
    13141 cttgataaat ggtgttccgt tagctgacat tgataataag actaaatttc aaaaaagttg
    13201 aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact aagtggcttt ttcacaacct
    13261 ctttttatct aataaccact gtttccaact caactaattt tgcccacgtt tcaatttgat
    13321 ctgttgttag atttagcgag acaacggtat gatgaccgcc acctgcctga atccatgaat
    13381 ggacaccttc atggaaattc ggtttagttt tccataacac acgtgcaaca ggtaaattag
    13441 gtgcttcagt cgttggttca aaggcttcta cttcattgat cagcagttta taatgcgtac
    13501 cgaaatctgc catcgatacg actacccctt ctcctgcttt cccatcaaat acaagacgcg
    13561 ccggatcttc tctatctccc attgataaag gagaaacaac gattcttggt ttcgtcgcag
    13621 caagcgtcgg atctacttcc atcatatggg attccaagat tgcttcttgg ccactagcta
    13681 gctcgtaagt ataatcttcc atgaatcctg tatctttccc tcgagccatg attttcagca
    13741 accgatcaat cgctgcagtt ttccagtcgc cttccccagc aaaaccgtat ccttctgcca
    13801 tcaaacgttg tacagctagt ccaggtagtt gtttcattcc atacaggtct tcaaaattag
    13861 tcgtaaaagc gttatagcct ccttcttcta agaaacggcg aataccgatt tcctgctgag
    13921 cttgtacttt tacatgttcc tcccatgtct tttcttcata atcaccatag tcgaaggtat
    13981 acaattcctt gtactcttca aataattgat cgacttcttc gtctgatacg cgatcgatta
    14041 cttgtacaag atctccgata ccaaaataat caacagtcca accaaattga atctgtgctt
    14101 ccactttgtc tccttctgtg acacccacgt ttctcatgtt gtcaccaaaa cgagccactt
    14161 ttattccaaa actttcatta taggctactg caacatccat ccaatcagca atctcttttt
    14221 ggatctcagg tcgtttccag tagccaacga cgattttatt ttgtttattt aatcgagcat
    14281 tgatgaaccc atattcgcgg tcaccatgag cggattgatt taaattcatg aaatccatat
    14341 cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc taaatgaagc aatggttttt
    14401 gtagaagctt tgtcccgcgg atccacattt ttgctggtga gaaagtatgc atccaagtga
    14461 tcacacctgc tacttcttct cgataattga cctctttcat gatttttgtg atcgtatcag
    14521 cagtaactgc taattcttga aggcggattg gataaggcaa ttggccattt tcattcagtt
    14581 catcgaccat ttcttgtgca tgtttcttta cttctcgcag tgcttcttcc ccatataaat
    14641 gctgtgatcc tacaacaaac caaaattctt tttctccaat attcaacata attagccact
    14701 ccttttattt gttttgtccg taatacgcat tttttccgtg ttttcgtaaa taatgtttat
    14761 ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag ctgtctggta aataaattca
    14821 tcttgcatac ttcatctagt acgactgcgt tcattaccgc actttgcgca tctttccccc
    14881 aagtaaatgg accgtgtccg tgtaaaagta cccctggaat tgctaaggga tcaattttgc
    14941 gttctttgaa tgtttctatg ataacttttc ctgtttcata ttcgtacccg ctgtcgattt
    15001 cctgttgtgt caaaaatcga gcacaaggta ccgatccata aaaagtatct gcatgcgtcg
    15061 tccccatcgc aggaacatcc aatcctgctt gtgcccagat cgtcgcccat gtcgagtgag
    15121 tatggacgat tcctccgatt tgaggaaatg aacgatataa aacagcatga gtaggcgtat
    15181 ccgaagaggg attgagttta ccttcaatga tatgattgtc caagtccaca acgaccatgt
    15241 cgtctgctgt catctcttca taactgactc cgctaggttt gataacgaaa tatcgtgttt
    15301 caggatcaaa agcgctcaca tttccccatg tatatttgac gagtccgtgt ttaggcaagt
    15361 caagatttgc ttggtatact tcttctttca gttgttccag cattttctat cactcctttt
    15421 tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc cctcacggta acgtctcata
    15481 aatatctgat agcctgctat ttcttctgct gaaggtgtga tggtgattcc ttggtcttgt
    15541 ccaaacacgt cttctgccaa gaagttttct aaagacttcc catgtcgatc catcatatag
    15601 gctgctaata acgcgattcc ccacgctcca ccttctccgg ctgtgtccat cactgtaact
    15661 ggtgcctcca tagcagatgc tagaatccgc tgtccaactt ctggtgtttt aaaaatcccc
    15721 ccatgcccca ctaaacgatc aatttggaca ttctcagatt tcaatatatc cattccgatt
    15781 ctcattgcac caaaagcact ggctaaatgg atccgcatga aattcgctaa atcaaaacgg
    15841 ctacctggtg tacgtacaaa taatggcctt ccttcattta ctcctgtaat gttttcaccg
    15901 gaaaaataac cataagaaag taatcccccg caatccgtat cgccttctag cgctttgttg
    15961 aataataccg tgaataattt atctctgctt atctccatac ccaaagaatt ggtaaattct
    16021 tcaaaaatac tgacccaagc attgatatcc gaagagcagt tattcgtatg aaccatgcct
    16081 actagttctc ctgaaggtgt agtgaccatg tcgatttccg gatggacatt ttctagttct
    16141 ttttctaata cgatcatagc gaaggcggaa gtaccagccg aaatgttccc tgtccctttg
    16201 gctacactgt tcgtagcgac cataccggtt cctgcatccc cttctggcgg acaaaccggg
    16261 attcctggtc tcaaatttcc cgtagggtcg atcagataag cccctgattc gcttaacttg
    16321 ccagcttctt ctcctgctac tagaacttta ggcaaaatgt gatccaagtc aatcgctacg
    16381 tgcgcttttt cgattaactg accaaaaata tttttcattt cttgatggta ggttttcgtt
    16441 gaaggatcga taggaaacat gcctgatgca tctcctacgc ctagtacttt ctcaccagta
    16501 agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat taatttcttt cacatgttct
    16561 tctttattca aaatagattg atacaaatga gcgatactcc atctctgcgg aatattgtat
    16621 tgaaattttt ccgttaatat tcgttctgct tcttcagtga ttgtatttcg ccaagtacga
    16681 aatggtacta gtaactgttc cttgctatca aatgctagat aaccgtgcat cattgcactg
    16741 aacccaatcg acccgattgt cgtaagattc tcgccataga tttcgaatac ttctgctgcc
    16801 atttttcggt aactgacgcg cagtcctttc caaatatctt ctaatgaata ggtccagata
    16861 ccattttcta gtttgttttc ccactcataa ctaccagcgg caataggagt aaaagatgaa
    16921 tcaatcaata cagctttgat cctagttgat ccaaactcaa tccctaaaga tgtacgacca
    16981 tctatgacat ctaacatact ttgcgcattt acttccttca taatgattta actccctcct
    17041 tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt acaatgatta ttgtatactt
    17101 ttgtacgtac atgtcaagca gttatttaca agaatacaat aaaaaacact tgttttgtac
    17161 gtacataagt tataaaatga aattgcttac aaaatctgat caaccgcctt ctcgaaattc
    17221 tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt cctcatcgtt tatttatttt
    17281 tttggcgcat ttggcggcat tctatttgga tatgatattg gtgttatgac cggcgcctta
    17341 ccttttttac agcatgattg gggtttggca ggtaaagctt ctttgatagg atggatcacg
    17401 tcttcggtca tgttaggtgc gatccttggc ggctctttat ctggtcttct ttctgataag
    17461 cttggacgac gaaaaatgat tcttctttct gcacttattt ttatggctgg ttctgtcctt
    17521 tctgcatctg cccctcacaa tggttcttat tttctgattg cagcaaggat tcttctaggt
    17581 ttagctgttg gcgctgcatc tgccctagtt cctgcttata tgtctgaaat ggctcctgct
    17641 agactaaggg gaagattgtc agggatcaat caagtgatga ttgcttctgg catgctcctt
    17701 tcctatgttg cagattattt gctaaaaggg ttgcctgaaa cgatggcttg gcgcgtgatg
    17761 ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg gtgttttagc tctaccagag
    17821 tcaccgcgtt ttcttatgca atctggccgc ttggaagagg caaaaagagt attgaactat
    17881 attcggacac caaaagaagc tgaacaggaa ttcgaacaaa tccagctgaa tgtcaaacaa
    17941 gaaaagacaa ccggaacttc ttggcatact ttgtttttag aaaaatatcg ttccttagtc
    18001 attgccggta ttggtgtagc agtattccag caatttcaag gagctaatgc tattttttac
    18061 tatattccgc tgatcgtaga aaaagcgact gggcatgcag cgagcgatgc attgatgtgg
    18121 ccgatcatcc aaggaatcat tttagttgct ggttctttgc tctttttggt tattgcagac
    18181 aagttcaatc ggcgaacttt attgaaaata ggcggtagcg tgatgggact ttctttcatc
    18241 ctaccagctg ttttaggaac tgtactggat gctcatacaa attcgttgtt gattctcttg
    18301 tttttatgta tttatgtcgc attttattct tgcacatggg ctccactgac atgggtaatc
    18361 gtaggcgaaa tattcccact agctgtccgc gggcgagcat ctggcctagc ctcttctttt
    18421 aattggattg gttctttcct agttggcttg ctttttcctg tgatgactgc ttctatgtct
    18481 caagaaatcg ttttcggcat ttttgggatc atctgctttt taggggtttt atttattcaa
    18541 gagatcgtac cagaaactcg tgggaaatct ttagaggaaa tcgaacaaag tgcatctaaa
    18601 aaaacttatc ctaaacgtat ttctatccat cattcttaaa gaggttgtga cagaagtggt
    18661 cagcttcaag aaataagaag gaaccgctta gtttccagtc acaaggaaca atttcaaaaa
    18721 acagcttctc atgtttgatg aatattgtga cttgtgacca gttggtagga gaacaccgtt
    18781 tagtggagag gtggtgaaag aaacgttcag ccttgagcga tgacaaaagc tctcacaaac
    18841 attgcgacga gtaaccgtag gagcaataag aggcggtaaa aacccgtctc ttatttttcg
    18901 ttttctttca ctgtttcccg ttcaactagt acaggttcat agagaatatt tggctgtgcc
    18961 tgtccttttt caatcgttgt gacgatccac cgtgcggcat cttgtcccaa cttttctttt
    19021 ggatgcgtca atgttgtcaa ttttttattc cctaaatggc ttaaagaaga atcgtctgtt
    19081 cctacgatcg ataggtcatc tggaatatga tagccatttt ctgtcaactt atctagcatt
    19141 aaactagcta tttgatcgtt atagcaaatg atgccatcta aagcttgttc ttctttcaac
    19201 cgttgtactg ttttttcagc gattgtttct cgtgtttccg tggtgaaagt aatgatatct
    19261 tccgtcaaaa aacgcacatt atactgttca cacgcgctga taaaaccttt catcctgtat
    19321 tttccttgaa gatcgtcgat ttttgtgatg aacaacagac gctgatgtcc tttttttatc
    19381 aaatattctg ctgccataaa accgactttc acatcatcta aacaaatata agaggcattt
    19441 aactcttcat aagcagcatt gatcatcacc atcggaatgc cttgttctct caaagcaaca
    19501 taaagtgcta agtttgggtt atactgatta cttttcgtag gctcaacgat caaaccatct
    19561 accccataag caatcatttt cttcaaacac tctttctcca attcgtgatc gttattcgta
    19621 ctggctaaaa gcaatgaata cccagattct cgtaattcat tttcgattcc tcggataatc
    19681 gacggaaaaa tataatcaga gagatatgtg gtgatgacac cgatcgtttt tgttctgttc
    19741 aatggtgttt tttctttctt atagctgtca tctacatatg tgcccgaccc tttttcttta
    19801 cgcagatagc cctcatttac caacacagcg attgcttgcc gaatcgtatg tctgctgacg
    19861 tcatatattt cttgcagctt gaattctgag ggaatggttg tatttacttc gtattcctca
    19921 cgaaggatct tttgttttat ctcttctgca atatcttgat attttgccat ttgccttgcc
    19981 cctttttatt tgtacgtaca tatatcataa cgagtctgca aggtttattc aagcaaaaat
    20041 caaggtttct tgtgctcata aggcatacgg aacaggttca ccaaattctg gaaatccttg
    20101 ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta ggattgtcta acggatcccc
    20161 tttttggttc ttctctggtc gagcatgata gatcaaaata tcaaagcacc cgtcttctga
    20221 aaccgtaaac gagttgtgac caggaccaaa ttgcttattt ttttcagaag aaacgaatac
    20281 tggctccgat aatttatgcc atgattcttt taataaaaga ttcttgtgct catcagccca
    20341 cagcaggccc atggcatagt tttcatctgt tgcactacca gaatacgtaa tgaatatttt
    20401 cccattgcga gtaaggactg ctggaccttc atttactgaa aaaccccgtt tttcccattc
    20461 atactcaggt atagaaagga ggatctgctt cccctttaac gtccaaggat tatccatttc
    20521 ggatagataa agattagaat tcccagtaat ttcaggatct tgttgggccc acacataata
    20581 tagctttcct tgagaagaga aagttgttcc gtctaaactg aaagtttccc ttttcgtttt
    20641 tatttgtccc ttttctatcc aatctgtatt cattggatct atatgctgat tttcaagtac
    20701 gaacatgcgg tgatgatgag attcatcacg tatagtttta tgattgcttg cagcaaagta
    20761 aatataccat tttccattga tataatggac ttcaggtgcc cagatcaatt cactcatcgg
    20821 tccttcttca tgagcctgcc atacaatcag gcttgcagca tgttctaatt cattcagtga
    20881 ctttgccctt cttaattcaa tcacttggta gcctggaacc gatcctgtaa aataataata
    20941 accatctgta tgtttatata cccaaggatc tgcccgttgc cgaacgatag gattattata
    21001 aagtgtcttc atcataacaa atttcctcac attttcttta ttctaattac attccaagag
    21061 tatttttcta tccctacatt taattcgtca tgtttttcta ttgaaaaata gtttatttca
    21121 ggttttattt tttctggttc ttcacgtgta tttttgtcca acagatcgct actggttaat
    21181 gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt caatcgttat cttttgttgt
    21241 tcatttgtgc gattgatcaa gaaaatagtc agctgattgc cgttatctac gatcacactg
    21301 tccacataag gaacattttt tatcttttca caatcataaa cagggcaaat gatttgttgt
    21361 ttcaatactt gacctttagc atatttcgat aagtgttgca tcacataata agttggattc
    21421 ctccatgctt gtcctttagc atctgtcagt attaaaggta ttgtgtttac taacaatgat
    21481 tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag ccagccctaa tgaaccaaat
    21541 aataatgtat cttccatagt aaagtaacac caatcgatgg gactaccgac ttcaaaatcc
    21601 ctatgtcttt tttccggttg ggcatgtaca ttccattcat caaacgctag atacattgtt
    21661 ttctcacttc tcaaatatcc ttttacataa tcacaagtag caacgatttc ttcaatttga
    21721 cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat catcacgctc atttttagga
    21781 ggtttagtca agtcttcttc atcatactta tcaatatagt tatgcaaagc taaataatct
    21841 acattctcat aagcttcttc taatgcgata cgatcccatt cagggtacga tggtaaacgt
    21901 ggcgtagagc ttccaactaa gattgtttca attgaatcat ccactaactt catgactttt
    21961 gaggcttcat ttgctaacct accatattca taagcactct tagcccccac ttgccatgga
    22021 ccgtctagct cattccctaa acaccataat ttaacgccaa aaggcttttt ttgcccattt
    22081 tttattctta aatcactcca gaaggttcct tttggaaaat tacaatattc tactaaattt
    22141 tttgcttcat caattccttt tgttcctaaa ttcaccgtga atatcggttt cgcttgtaca
    22201 tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat ttggttctat acctcgccat
    22261 gctaaatcaa ttttttttgg tcgttgttct ttcctaccta cagtatccac ccaatcataa
    22321 ccagatgtaa agtttcctcc aggatagcga atgacaccta attctaattc ctttacataa
    22381 gagaggacat cttgtctaaa cccgtcacca tcagctgttt catggcccgg ttcataaatc
    22441 cctgtataga cgacacttcc catatgctca acaaatgagc caaacagccg agaactgatc
    22501 tctcctaccg taaaatcctc tctgattttt actttcactt cttcgtccaa cagctttccc
    22561 tacctttcat ttcctatagt caagataata gatacaagtt tccatacgcc accagcaaag
    22621 ataaaaataa aaggagggaa aagaaatatg agcagtacaa atcctaataa caatacgcaa
    22681 caaagaatat ttctcaaaaa atcaaaaaat actttccatg cactttgttc aaaaatttgt
    22741 aaaaatgtga tatctttgtt ttcagaaatc acatatatcc aggataaaag aaacatggaa
    22801 taaataagta aaactacata tccactaatt gaaagtacga ttgtctgttc ttctaataat
    22861 tgattaacaa gaaaataaat aatgatcgtt aacagactaa ggaacaagtt cttaccaaaa
    22921 tgttgcttta ttccttgaaa gaaaaaataa atgatatttc ctgatccctc acctttccat
    22981 tgcgccatta cataaaaaaa cagcacatgt agcaggcacg atagtaataa caggcaaaca
    23041 cacgattacc cagagaatac ctaataagat gtaatcagaa accacaatca actgtccata
    23101 aagtttagtc tcaactattt tcatcttcct cactctttca ttccagacat agctgttcct
    23161 tgaacaaaat atttctgtgc aaaaatataa accactagta atggcagcat tgaaataacc
    23221 gttccagcca ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa agctaaccct
    23281 gctggtaaag ttctcatttt tgaactactt gtcaatatca atggataaag caagtcattc
    23341 caactattca tcagcgtgaa aatccctaat gataaaagag taggtttgct caatggcaac
    23401 atgattttca aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa
    23461 gaatctggta aagtaataaa aaaggaacgc atcatgaaga taccaaatgc agttgtcata
    23521 cgaggaatga tcaaacctgc ataagtatca agcatgccaa gcaaattgac ttcaataaat
    23581 agaggaatca tgaagacttg gaatgggatc atcattgtca gcaaaacgaa atagaacaaa
    23641 actgattttc ctttgaaacg catacgagca aatgcatacc ccgcaagaga atcaaagaat
    23701 acagaagtga tcactgtccc tccagcaaaa atcactgtat ttttgatgta atctaataaa
    23761 gggatcgttg tccaaacctt aatatagtta tctagggtat agctatctgc aaatattcta
    23821 ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa
    23881 ggaaacgcaa taaccaacaa tataactagc atactaatcg ttcgaatgat ttttttacca
    23941 ctaactgctt ttcgactatt cacagtaact gtaatcgagc tttctggtgc catacaggtt
    24001 tccctcctat ttcatttgtt tttctttccg tgacatgtac acattagtgg agatagctat
    24061 agttgcaaca atgataaaca aaaccgaaga cagtgctgat gcatagccca attcataagg
    24121 tgctgtaaat cctcgactgt aaatatattg aacagctgtt tctgtcttaa attgtgggcc
    24181 accagcggtt gcaacatata cttggtcaaa cacttgcatc gaaccaatca gattcgttaa
    24241 aatacaaaaa cctaaggagg gaacgatctg aggtagcgta atgttaaaaa actgctgtcg
    24301 atttgttgca ccatccattt gagcagattc atacaaagag ggagagattc cctgaatagc
    24361 agtcaagaga atcgtcatag taacaccaaa attcttccaa acagtcatga tggctaccgt
    24421 cggcatagca agggttggat cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa
    24481 caagtaagga atcgccccta cattcggatc aagcaacata gaaaaaataa tcgcaattga
    24541 cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt
    24601 atttttagat aatgctactg ctgctaataa accaacgata atttgtgttg gcgtttcgag
    24661 tacggtgaaa tataaagaat gaatcaacga attaactgct cgttcatcac taaatatacg
    24721 tgtgaaattt tcaaaactta caaaatttct tgatgtaaag aaaatattca aatctgtaaa
    24781 actaatagca aatgttccta aaagcggtat gacagtaaat aataataaaa ctaaaaaaga
    24841 tggagttaaa aacaaatacg cagcacgatc aggacgattc caatatttgg tgaatccgtt
    24901 catatagtcc acctcatctt tcattttgat aaaatataca atcttggttt aaacgaatct
    24961 gaataagata tccgaacaat cgctgtgatt tttgcaataa cacctatctg caaatactag
    25021 gtcgatcatt cagatatctg tatcttgcca acagtctatc ctgtataaag ttctgtacta
    25081 tttttgcagt aattgattga tggcatcatc tgcatcgttc atgagtgtct caggatcttc
    25141 tcctgctaat aatttttcaa tcagtggatt gatgatatcg ttattgatcg cagagatttg
    25201 ggtaaaacct ggtaaatatg gctctgcata gtccatttgt ttcgataatt cagatacaat
    25261 aggatcattt tttacttctt catcttttgc tactgaatcc aagtaggcag gaaaaccatt
    25321 ttcgatactc cattttttac caatttcagt tgtattccaa tatttcaaaa actcatagac
    25381 agcttctttt ttattattat ctgtacttgc cggaatggca tatcctacgc catctaaaat
    25441 agctgttttt ttgtctgaat cactcccttg tggtaaggta gtcacattat aatcaatctc
    25501 gtttgctttc aatccgttat ttaaccatgg cccattgatt tcaatggcta gttgaccagc
    25561 attcattaaa ttgtccattt cggcgccaga aatcgattct ggaccagtct tgtctacatg
    25621 gatcattttt tggatcgctt ttaacgtttt aaggttctca ggagacgcaa attcagactt
    25681 agtataatca tcgttcacta atttaccgcc atttgctaac atccagttat ataacggtgc
    25741 agttccatcc ttattgaaga caaaacctga tacattttta ctactatcag ttaattttgg
    25801 tgccatttct gtcaattctt cccaagtagt aggtggttgt tcagtatcta atccagcttg
    25861 tttaaacaaa tcattattcc aatacatata catgccttgt acctgcatgg ggacaaaata
    25921 ctgtttacct tctacttcac ctaattcaat agccgtttct ttaaaatcag atttatctac
    25981 tccatcatat tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta catactgtgc
    26041 aaaatctgga taatttaatg caataaagtc aggtgctttt ttagaagaaa tcgctgtagg
    26101 aagtttttca ttcaaattgg cccaagtcat gatatccatt tcaattttga tgtttttatc
    26161 atttgtttta ttaaattcat taacgatttc ttgcaatatt tcaccatcag atgcagtaaa
    26221 cccattccaa aaagtcaaag tcacttcttt atcagaatca gattctgctg aacttccgcc
    26281 accgcaagct cctaaaatac ctgttgcagc taccaacaaa gagccaatta accatttttt
    26341 acctttcatt ttttgttcct ccttcatctg ataaacgaca tctgaccaca ttccaagata
    26401 gcggtttcaa taaacaattg attttcccat cttcagcttt tacatttgtt gaatgacttg
    26461 gttttacttg ttcactttta ggaagattag ttgcttttgt atcaaatcca ctcatttcag
    26521 aaaaatcaat cactccctct aatgataatt cagacaagtg aacagttaga ttcatttctt
    26581 cttctccacg gttaactgca aatatgacaa gttctttgtc atttattacg gctatacttt
    26641 ctaaatacgg cacttcttca aaatcttccg tcgcatagtt tgctgaacga atttgtgggc
    26701 ttaacacctc tccttgtcca tacacagcag cttgcataaa tggataatag atcgtctgtc
    26761 tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat aacatttacc aattgagcaa
    26821 gacaagcaat ctttactcta tctgcatgtt tcaataaagt gattaataga cagcctaata
    26881 gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg aggagcaatc tgccaaggcg
    26941 ctgcttgctc atcttgttcc gctgaatgat accaaatatt ccattcatca aaggacaggt
    27001 tgatctgctt cttactatgt ttcttcgcct tgactgtatc gcacatcgct gtcacttcag
    27061 aaatgaaccg atccatgtct aaggaacgtg caagataatg agaaagatca tttttttggt
    27121 tgccataata ttgatgcaga gacaaataat ctatttgatc gtacgcttga tcaagaactg
    27181 ttaactccca atcaccaaat gtaggcattt gccgattcga acttccgcat aaaaccacct
    27241 caattgaatc atctacgagt ttcatcgcct tagccgtttc ctctgctaac cgtccatatt
    27301 cttccgctgt tttatgtcca atctgccaag gaccgtccat ttcattccct aaacaccatg
    27361 tcttaattcc aaaagggctc tcttgaccat tcgaacgcct taaatcactc caataagttc
    27421 ctttaggaaa attacaatat tcgactaaat tacgagctgc atctactcca cgagtaccca
    27481 gattgacagc catattgatt tcggccccca catgctgaca ccagttataa aattcatgaa
    27541 tcccaacttc attcgtttca attgtccgcc aagctaaatc caaccttcta ggtctatttg
    27601 ctatgggccc aatactgtct tcccaacgaa atcctgacac aaaatttcca ccaggatatc
    27661 taatcatggg aatgttcaat tcttttacca agtgaatgac gtcttggcga aatccacttt
    27721 tattagctga tggatgatct ggctgataaa tcccttcata tactgctcgt cccatatgtt
    27781 caataaatga actgtacaac cgcggatcaa ctttgcctac acgcttgaca ctactaatca
    27841 acattgtggc ttccataact gacctccttt aatttataca tgaataatac acctcgttga
    27901 aagcgttgtc aataatataa atgttaaact tacttatttt ttatcgattt taattaacca
    27961 aaatattaat aatgattaaa acaaataatt taatataact tttattaaca aatatgttaa
    28021 tatataagaa aaacctttta agaggtgata tatatgcaac tacaaataaa cgaagaatcc
    28081 ttaccggtat atgaagcatt agctagtaaa acaaggatca agatcatcca gctactttcc
    28141 aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg gagtgagcag tgctatcacg
    28201 acgatgcacg tgaaaaagct agaggaagca aacatcatca aaacagaaaa agtaggacaa
    28261 caaaaaattt cgagccttcg tgtcgataaa atcgatatca gttttccaga aaaaatattc
    28321 aatgcttttg atacgaaaga aacatcgata cctattggtc actatacaaa ttatgcaatt
    28381 gaaccaacat gcggtttagc tacgatccat gattttatcg ggaaagtgga tgaaccacgc
    28441 tattttatgg atcctcgaag gatggatgcc cgcattctct ggtttaccag cggatttgtc
    28501 gagtatcagg cacctaattt tttaaataca gaggatacat tggagatgtt ggaagtttct
    28561 gtggaaattt cttctgaatt tcctttttca aatgacaatt ggccttcaga tatcactttc
    28621 agcctaaatg gtgtggagct aggaacatgg acgagccctg gagattttgc tgatatccgc
    28681 ggtaaatata cgcctgattg gtatccagat aatcttaatc aatacggctt gctgaagacg
    28741 atccgaatta ccaaacactt gacgaatatg aatggagaac ccttgtcaaa catcacgatc
    28801 aacgacatcc ctaaagagca agacacttgg catttgcgaa tcgaagtgaa agacgatgcc
    28861 aaacatgtgg ggggctgtac tttatttggt aaaggattcg gtaattacga tcaggatatc
    28921 aaattaaaag tttactacag ctgatatttt tcttttctat tttataaaac aaaaacagcc
    28981 aatccctatg aagggacggc tgtttttgct ttattgttat ttccagaaat catccaaaac
    29041 ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac catgcttcga ttttttcttg
    29101 tgcttgagcg ggaacttctt ttccttctag ataatcatca atttcattat atgtgacacc
    29161 tagtgcgact tcatctgcaa tcattggttt cccatcttca agatctgcag tcggaatttt
    29221 tgtgtataat ttttccggtg cattcaatgc ttgaagcaat tgtttccctt gtcgtttgtc
    29281 caaacggaat aatggcaaaa tgtcggctcc accatcacca aatttcgtga agaatccagt
    29341 aatgttttct gcagcatgat ctgtccctaa tacggctcct gctctttctc cagctacggc
    29401 atattgcgta atcatacgtt gacgtgcttt gatatttcct ttgttaaaat cgctgattcc
    29461 cacacctgct tcgcttaata cttgtacttg tgcatcaaca gctggtttga tatttactcg
    29521 cagactaaca tcgggctgga taaagtcaag agcagctttt gcatcttctt catctgcttg
    29581 ttcaccataa ggaagacgaa cagcgataaa ttgatagttt tcatcttttg tttcttcacg
    29641 catttcttcc atcgttagtt gcgccaaacg gcctgctaaa gtagaatctt ggccgccgct
    29701 gatacctagt acaaaagttt taagaaacgg atattttttc aaatatgctt tcataaagtc
    29761 aatactttta cgtatttctt cttttggatc aattgttggt ttgacaccta attcattgat
    29821 aatttctgtc tgcaggctca tttattctcc tcctatgctt ctgtatgaag ttctgctacg
    29881 ctttttttca ttcgttccat gatagccatt ttatggttcc agcattcagt agacagatcg
    29941 actgggtatt tttgcggatt caagtctcgt ttatactctt cccatagtga atctagattt
    30001 tcttttgtat attctttgat ttcgtttagt gcaggtaatt cgtaaactct ttttccttcg
    30061 ataaagatat cttgtaaaac tgggcgagct gtaaagtctc gaacagtttt attgataaac
    30121 gtatgcactg gatggaacat gaagatagct tcttcttctc gagggtcttc atcccataat
    30181 gtcacgtagt ctccttcaga tttcccatca aaatttcgag tgatgcgcca cacttgtttt
    30241 ttccctggag tagttacttt ttctgcattg cttgataact tgatcgtatc catcattttt
    30301 ccttcatcat cttcgatcga tactagcttg aatactgcac caagggctgg ctgatcatag
    30361 gctgtgatca attttgtccc aacaccccat acatcaattt tagctttttg catctttaaa
    30421 ttcaaaatcg ttgcttcatc caagtcattc gaagcataga tttttgcttc cgtaaatccg
    30481 gctgcatcta attgttcccg aacacgctta gatatatagg ccatatctcc actgtcgatg
    30541 cgtacgcctt ggaagttgat tttgtcacct aattctttcg ccacgcgaat cgcacttggc
    30601 acacctgatt tcaacgtatc ataagtgtcg actaagaaca cgcagtcttt atgggtttta
    30661 gcgtaggcca taaatgcttc ataatcattt ccataagatt ggactaacga atgagcatgg
    30721 gtcccgctag ctggtatccc aaatatcttc ccagcacgta cattgcttgt tgcatctgct
    30781 ccgccgatat atgccgctct agttccccag actgctgcat ccagttcttg tgcccgtcta
    30841 gttccaaatt ccaatagtgg atcttctccg atgacagatt tgatacgagc tgcttttgtc
    30901 gcgatcaacg tttggaaatt gaccatattc aacagtgctg tttctactaa ttggcagtgc
    30961 gctaaaggcc cttccacttg gattaatggt tcatttgcaa aaactagttc tccttcacga
    31021 gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat actccaaaaa accttcagga
    31081 tagacttcta atccacgcaa gtaagcaata tcggaatcat tgaacgtcag attctctaaa
    31141 taattgacta accgttccaa tcctgcaaaa accgcgtatc cgtggttaaa aggcatttct
    31201 cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac ctaattccca gtatgtctgc
    31261 atcatattga tttgatatag atccgtatgc agcgtcaaac tgtcgtctgg gtaagtatta
    31321 ttcatcttgt attactcctc ttggcaagtt ttcatttcct acccattata acaaaaaaaa
    31381 ttaataggaa aagaaatctt ccattttttc tgaaaaagca catgttttac ggatatttct
    31441 tgctaaaaat gaactccatc tgctaaaact atcctttctt attggtattt aacctaaacg
    31501 agaaagaata cttattttat tcaatgtatc cgagacacga agaaattagc gttttctatt
    31561 tttcttactt gataaaataa gtagattttc ctactacaat cgtagccgtt aagataccga
    31621 tgattgaaca aatataacca aatgtctcta caacataatc aatggcaaaa gtttttgaag
    31681 caataatacg tctttccttt tttgcttctt atccccaaag aaacggatat aacttattat
    31741 gtattgaatt tttttggaaa ggcgaattat gacaagatat accttgcgtt gaaaaaactc
    31801 gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta agaaagcagc tgtcaataga
    31861 atggaaacaa aaatggcgag acatcaatca gtctcgcctt gttttttatt gtatacatta
    31921 gatatggagc aggttaccat aaagatgatt attagtcgtt attaaataga atccttcgta
    31981 tttttttgtt ctatcactac aatatcttta tttggacgtt tgaattgtat tccggcacgt
    32041 tctagctcct gtttcactcc tacatggact ttgacatctt ctcgtgtcat ttttaaaacg
    32101 tgatgaatcg catagtcgat ttcttgcctt gtgatttttt tcttcgtatt gatcatcacg
    32161 acttgctttt tatcatatgt gtctaccaat agcagttgtg ttcgatctaa cgaaaataat
    32221 ttgtaataat taatagccgt attcccaaaa aagaattcca ctactttagg ataaaatgtc
    32281 ttaatattta aattactatt cacaggtgtt tcgattagct tcatctcatt cctcttttct
    32341 atatagtcga tttagatcca atcttaagtt atgttaccat ctttcctagc attaaggaat
    32401 cggttacaat tgaaccttta aagacaaaag gatttacctt ttatcactta atagtataac
    32461 ataatttcgt gagaaaaaaa tacaattttt tcttgaccat ctcgttacgt aaaggattat
    32521 gcttacattt gtcaggagga aaaataatgg agtacacaat aaagaaaatg tcagagatat
    32581 caggagtcag tccgagaacc ttgcgctttt atgatgagat tggactgcta aaaccagcta
    32641 ggatcaattc ttcaggttat cggatttatg gcaaaaaaga agtcgatcgt ctgcaacaca
    32701 tcttatttta tcgaacaatg gcattcaagt tggatgatat ccaagaggtc ttagacaatc
    32761 catcttttga ccaccagaaa gctttaatca agcatcagca aatgttgctg gaaaaacggg
    32821 cacaaatcga taccctcttg acaactgtcc aacagacact tgatatgtac gaaggaggaa
    32881 gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca acaaaagttg aaagaaaatg
    32941 aagaatccta tggacaagag attcgacaaa aatacggaaa aaaaaaggtt acagcatcca
    33001 atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga aatgcaaact gttgaaaagc
    33061 agatgttatt tgatctcact ctttacttaa aagaaccaga agatgaaagc ttagcagacc
    33121 gtatcttcca agctcataag aagtggttct cttacagctg gcctgaatat cagccaaaag
    33181 cgcataaagg actaggaatg atgtacgaag ccgatgaacg gttcactgca tattatgacg
    33241 agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat tatccaaaaa aaggcgtaat
    33301 cttttacatg atccaacaaa aaagtacacg taaaatgacc taataacaaa acagcagaca
    33361 cccaagttag atttttatgc taacttttgg tgtctgctcc attctttcac aagttttttc
    33421 atcctacttt aaatatattc atcgttgaat aaacgtataa ggtattttca ctttatcctt
    33481 tttttctgta agaaacaatt gaaatgccat ttctccgcaa gcttccagat ggtggtcgat
    33541 ggttgggaaa ccaagaagct cgctgatcag caaattatcg cgtccaatca ccactggtgt
    33601 cttatcttgc agatagcttt gcaagatgcc tgctgcaacc atatccgaat ttgtaaagac
    33661 accatcaact ccaagctctt taaaaaagaa gcctgcttgt tttcctttct ctgcatctat
    33721 acaatcccaa aaaacaagtg attcatcaaa agatggaaaa agttgatgac atagctgcaa
    33781 agtgattttt gagttataac ttagcctgcc acttcgcccc aacgtgatgc ccaattgctt
    33841 tataccttgc tgcttcaaat aagtaagacc ttctgtaatt gattgttctc gatcaataaa
    33901 gacacagctt gcctgttttc ccttgatttc ttcacaaaaa actagaggtc catatttttg
    33961 atatgccaat aaatcatcta gtgtattggc acgagacgta atgatcaatc catcgaaagc
    34021 tttagctgca aattcttcaa gataccttcg ctctttttct ttatgatagc ccgtcggtaa
    34081 aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg atcccactga tcaactgatc
    34141 aaaatacgga tgattggtat aaggcagaat cactccaatg cttttcgttt ttccataact
    34201 taactgtctg gcatttgcat tagggatata atcgacttct ttcatgaccg caagtatttc
    34261 tttccttttt tcctcatcta catatggatg tccattgatt acacgtgaaa tagtagaaac
    34321 tgaataacct gtgagctttg cgatatctcg aatgtttgcc actgctgctt cgccacctat
    34381 acaaaaagat tttcttgtaa atagtctacc acagctggac cgcattcaaa aaactgatac
    34441 atttttcctt catgcgaaaa actgatccgc tgcccgcccc aatatatatc ttttgtcaaa
    34501 ttttcgattt cattcattgg tatttctttt actatattcc tattcaaccc ttgaaaactg
    34561 tacaacgtta atacggatcc cttgaactcg acaaacgcat aaaaataatt gttcgttgtg
    34621 ccatctgtta tctctacata agtattcatt gcaagccctc cttttgatac tttgagtata
    34681 gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc aaagaaaagt tttattcctc
    34741 tttcattatt accaaagtct
//
LOCUS       DAYYAO010000023        34449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000023 DAYYAO010000000
VERSION     DAYYAO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.024"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHARI_001010"
     CDS             352..1041
                     /locus_tag="ACHARI_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8442073.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHARI_001011"
     CDS             1028..2236
                     /locus_tag="ACHARI_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8442074.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHARI_001012"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHARI_001012"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8442075.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHARI_001013"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHARI_001013"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8442076.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACHARI_001014"
     CDS             complement(6339..7034)
                     /locus_tag="ACHARI_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8442077.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHARI_001015"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHARI_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8442078.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACHARI_001016"
     CDS             complement(8233..8424)
                     /locus_tag="ACHARI_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8442079.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACHARI_001017"
     CDS             complement(8445..9335)
                     /locus_tag="ACHARI_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8442080.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACHARI_001018"
     CDS             complement(9322..10089)
                     /locus_tag="ACHARI_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8442081.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHARI_001019"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHARI_001019"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8442082.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHARI_001020"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHARI_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8442083.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHARI_001021"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHARI_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8442084.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACHARI_001022"
     CDS             14660..15043
                     /locus_tag="ACHARI_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442085.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHARI_001023"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHARI_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8442086.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACHARI_001024"
     CDS             complement(15987..16805)
                     /locus_tag="ACHARI_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8442087.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHARI_001025"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHARI_001025"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8442088.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHARI_001026"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHARI_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8442089.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17934..19268
                     /gene="dnaA"
                     /locus_tag="ACHARI_001027"
     CDS             17934..19268
                     /gene="dnaA"
                     /locus_tag="ACHARI_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8442090.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19466..20596
                     /gene="dnaN"
                     /locus_tag="ACHARI_001028"
     CDS             19466..20596
                     /gene="dnaN"
                     /locus_tag="ACHARI_001028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8442091.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20825..21067
                     /gene="yaaA"
                     /locus_tag="ACHARI_001029"
     CDS             20825..21067
                     /gene="yaaA"
                     /locus_tag="ACHARI_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8442092.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21054..22178
                     /gene="recF"
                     /locus_tag="ACHARI_001030"
     CDS             21054..22178
                     /gene="recF"
                     /locus_tag="ACHARI_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8442093.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22175..24121
                     /gene="gyrB"
                     /locus_tag="ACHARI_001031"
     CDS             22175..24121
                     /gene="gyrB"
                     /locus_tag="ACHARI_001031"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8442094.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24141..26612
                     /gene="gyrA"
                     /locus_tag="ACHARI_001032"
     CDS             24141..26612
                     /gene="gyrA"
                     /locus_tag="ACHARI_001032"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8442095.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26848..27147
                     /gene="rpsF"
                     /locus_tag="ACHARI_001033"
     CDS             26848..27147
                     /gene="rpsF"
                     /locus_tag="ACHARI_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8442096.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27197..27709
                     /gene="ssb"
                     /locus_tag="ACHARI_001034"
     CDS             27197..27709
                     /gene="ssb"
                     /locus_tag="ACHARI_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8442097.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27735..27971
                     /gene="rpsR"
                     /locus_tag="ACHARI_001035"
     CDS             27735..27971
                     /gene="rpsR"
                     /locus_tag="ACHARI_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8442098.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28114..30087
                     /locus_tag="ACHARI_001036"
     CDS             28114..30087
                     /locus_tag="ACHARI_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8442099.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30093..30545
                     /gene="rplI"
                     /locus_tag="ACHARI_001037"
     CDS             30093..30545
                     /gene="rplI"
                     /locus_tag="ACHARI_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8442100.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30819..32186
                     /gene="dnaB"
                     /locus_tag="ACHARI_001038"
     CDS             30819..32186
                     /gene="dnaB"
                     /locus_tag="ACHARI_001038"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8442101.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32966..34258
                     /locus_tag="ACHARI_001039"
     CDS             32966..34258
                     /locus_tag="ACHARI_001039"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8442102.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10504 a         6696 c         6824 g        10425 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17701 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17821 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17881 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17941 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    18001 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18061 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18121 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18181 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18241 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18301 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18361 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18421 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18481 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18541 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18601 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18661 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18721 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18781 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18841 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18901 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18961 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    19021 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19081 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19141 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19201 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19261 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19321 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19381 ttttccacag aatcaacatg ccctattact tttattattc tttttaacta ataataaata
    19441 aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca
    19501 ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg
    19561 tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc
    19621 aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg
    19681 ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac
    19741 atttacttta gaagttttag aaaataaaca agtagcgatt acttctggaa aagcgaattt
    19801 tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa
    19861 ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt
    19921 ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc
    19981 tttattggct gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca
    20041 agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc
    20101 attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa
    20161 aacagaaacg atgtatttct attcccgttt gctagaagga aactatcctg ataccaatcg
    20221 tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc
    20281 gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat
    20341 tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag
    20401 cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat
    20461 gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat
    20521 tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc
    20581 tgtacgtaca aattaacaat gattttcatt gttcttgaaa atttaattga aaagaaatag
    20641 aaaaaataaa attttaaaca gaaggcccta aaatctcttt taaggctttc tgtttatttt
    20701 tgtttaaatc acctttttta gattaaatcg tcttatatgg aaaaatatat ggataaattt
    20761 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
    20821 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
    20881 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
    20941 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
    21001 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
    21061 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
    21121 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
    21181 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
    21241 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
    21301 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacaaaaaa
    21361 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
    21421 tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca
    21481 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
    21541 atttaaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
    21601 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
    21661 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
    21721 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
    21781 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
    21841 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
    21901 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
    21961 ttaaagaaga gacgggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
    22021 atgatagtcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
    22081 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
    22141 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
    22201 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
    22261 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
    22321 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
    22381 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
    22441 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
    22501 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
    22561 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
    22621 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
    22681 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
    22741 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
    22801 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
    22861 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
    22921 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
    22981 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
    23041 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
    23101 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
    23161 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
    23221 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
    23281 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
    23341 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
    23401 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
    23461 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
    23521 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
    23581 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
    23641 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
    23701 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
    23761 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
    23821 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
    23881 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
    23941 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
    24001 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
    24061 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
    24121 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
    24181 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
    24241 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
    24301 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
    24361 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
    24421 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
    24481 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
    24541 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
    24601 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
    24661 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
    24721 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
    24781 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
    24841 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
    24901 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
    24961 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
    25021 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
    25081 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
    25141 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
    25201 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
    25261 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
    25321 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
    25381 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
    25441 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
    25501 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
    25561 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
    25621 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
    25681 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
    25741 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
    25801 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
    25861 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
    25921 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
    25981 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
    26041 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
    26101 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
    26161 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
    26221 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
    26281 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
    26341 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
    26401 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
    26461 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
    26521 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
    26581 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
    26641 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
    26701 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
    26761 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
    26821 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
    26881 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
    26941 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
    27001 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
    27061 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
    27121 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
    27181 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
    27241 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
    27301 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
    27361 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
    27421 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
    27481 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
    27541 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
    27601 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
    27661 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
    27721 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
    27781 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
    27841 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
    27901 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
    27961 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
    28021 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
    28081 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
    28141 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
    28201 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
    28261 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
    28321 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
    28381 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
    28441 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
    28501 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
    28561 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
    28621 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
    28681 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
    28741 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
    28801 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
    28861 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
    28921 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
    28981 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
    29041 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
    29101 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
    29161 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
    29221 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
    29281 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
    29341 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
    29401 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
    29461 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
    29521 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
    29581 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
    29641 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
    29701 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
    29761 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
    29821 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
    29881 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
    29941 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
    30001 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
    30061 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
    30121 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
    30181 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
    30241 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
    30301 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
    30361 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
    30421 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
    30481 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
    30541 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
    30601 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
    30661 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
    30721 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    30781 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    30841 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    30901 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    30961 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    31021 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    31081 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    31141 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    31201 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    31261 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    31321 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    31381 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    31441 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    31501 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    31561 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    31621 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    31681 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    31741 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    31801 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    31861 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    31921 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    31981 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    32041 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    32101 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    32161 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    32221 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    32281 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    32341 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    32401 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    32461 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    32521 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    32581 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    32641 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    32701 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    32761 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    32821 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    32881 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    32941 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    33001 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    33061 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    33121 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    33181 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    33241 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    33301 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    33361 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    33421 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    33481 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    33541 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    33601 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    33661 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    33721 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    33781 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    33841 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    33901 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    33961 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    34021 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    34081 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    34141 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    34201 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    34261 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    34321 cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg
    34381 tctcttgaac actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct
    34441 ttttccgaa
//
LOCUS       DAYYAO010000024        34388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000024 DAYYAO010000000
VERSION     DAYYAO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.025"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..2092)
                     /locus_tag="ACHARI_001040"
     CDS             complement(8..2092)
                     /locus_tag="ACHARI_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8442103.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(2234..2356)
                     /locus_tag="ACHARI_001041"
     CDS             complement(2234..2356)
                     /locus_tag="ACHARI_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442104.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(2617..2976)
                     /locus_tag="ACHARI_001042"
     CDS             complement(2617..2976)
                     /locus_tag="ACHARI_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8442105.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3006..3347)
                     /locus_tag="ACHARI_001043"
     CDS             complement(3006..3347)
                     /locus_tag="ACHARI_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442106.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3344..4018)
                     /locus_tag="ACHARI_001044"
     CDS             complement(3344..4018)
                     /locus_tag="ACHARI_001044"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8442107.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5194..6492)
                     /gene="asnS"
                     /locus_tag="ACHARI_001045"
     CDS             complement(5194..6492)
                     /gene="asnS"
                     /locus_tag="ACHARI_001045"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8442108.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6532..7722)
                     /locus_tag="ACHARI_001046"
     CDS             complement(6532..7722)
                     /locus_tag="ACHARI_001046"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8442109.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7743..8270)
                     /locus_tag="ACHARI_001047"
     CDS             complement(7743..8270)
                     /locus_tag="ACHARI_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8442110.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8317..11106)
                     /locus_tag="ACHARI_001048"
     CDS             complement(8317..11106)
                     /locus_tag="ACHARI_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8442111.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11253..11453)
                     /locus_tag="ACHARI_001049"
     CDS             complement(11253..11453)
                     /locus_tag="ACHARI_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8442112.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11906..12226)
                     /locus_tag="ACHARI_001050"
     CDS             complement(11906..12226)
                     /locus_tag="ACHARI_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442113.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12729..13847)
                     /locus_tag="ACHARI_001051"
     CDS             complement(12729..13847)
                     /locus_tag="ACHARI_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442114.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14080..14202
                     /locus_tag="ACHARI_001052"
     CDS             14080..14202
                     /locus_tag="ACHARI_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442115.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            14318..15625
                     /locus_tag="ACHARI_001053"
     CDS             14318..15625
                     /locus_tag="ACHARI_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8442116.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15746..16945
                     /locus_tag="ACHARI_001054"
     CDS             15746..16945
                     /locus_tag="ACHARI_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8442117.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(16972..18240)
                     /locus_tag="ACHARI_001055"
     CDS             complement(16972..18240)
                     /locus_tag="ACHARI_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8442118.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(18296..18362)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(18410..19051)
                     /locus_tag="ACHARI_001056"
     CDS             complement(18410..19051)
                     /locus_tag="ACHARI_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8442119.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(19460..19963)
                     /locus_tag="ACHARI_001057"
     CDS             complement(19460..19963)
                     /locus_tag="ACHARI_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8442120.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(20086..20850)
                     /locus_tag="ACHARI_001058"
     CDS             complement(20086..20850)
                     /locus_tag="ACHARI_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8442121.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            20958..21233
                     /locus_tag="ACHARI_001059"
     CDS             20958..21233
                     /locus_tag="ACHARI_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8442122.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            21793..22743
                     /gene="yidC"
                     /locus_tag="ACHARI_001060"
     CDS             21793..22743
                     /gene="yidC"
                     /locus_tag="ACHARI_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8442123.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(22789..23190)
                     /locus_tag="ACHARI_001061"
     CDS             complement(22789..23190)
                     /locus_tag="ACHARI_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8442124.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(23204..24013)
                     /locus_tag="ACHARI_001062"
     CDS             complement(23204..24013)
                     /locus_tag="ACHARI_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8442125.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(24000..24890)
                     /locus_tag="ACHARI_001063"
     CDS             complement(24000..24890)
                     /locus_tag="ACHARI_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8442126.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(24903..25382)
                     /locus_tag="ACHARI_001064"
                     /pseudo
     CDS             complement(24903..25382)
                     /locus_tag="ACHARI_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(25558..26499)
                     /locus_tag="ACHARI_001065"
     CDS             complement(25558..26499)
                     /locus_tag="ACHARI_001065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8442127.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(26496..27491)
                     /gene="lacD"
                     /locus_tag="ACHARI_001066"
     CDS             complement(26496..27491)
                     /gene="lacD"
                     /locus_tag="ACHARI_001066"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8442128.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(27484..28680)
                     /locus_tag="ACHARI_001067"
     CDS             complement(27484..28680)
                     /locus_tag="ACHARI_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8442129.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(28698..29426)
                     /locus_tag="ACHARI_001068"
     CDS             complement(28698..29426)
                     /locus_tag="ACHARI_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8442130.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(29867..30841)
                     /gene="lacD"
                     /locus_tag="ACHARI_001069"
     CDS             complement(29867..30841)
                     /gene="lacD"
                     /locus_tag="ACHARI_001069"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8442131.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(30838..31296)
                     /locus_tag="ACHARI_001070"
     CDS             complement(30838..31296)
                     /locus_tag="ACHARI_001070"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8442132.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(31293..32756)
                     /locus_tag="ACHARI_001071"
     CDS             complement(31293..32756)
                     /locus_tag="ACHARI_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8442133.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(32738..33670)
                     /gene="pfkB"
                     /locus_tag="ACHARI_001072"
     CDS             complement(32738..33670)
                     /gene="pfkB"
                     /locus_tag="ACHARI_001072"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8442134.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            33812..>34388
                     /locus_tag="ACHARI_001073"
     CDS             33812..>34388
                     /locus_tag="ACHARI_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8442135.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT        10012 a         7428 c         5864 g        11084 t
ORIGIN      
        1 aggacgttca ttcgtcctct ttttttacat gcaagcttcg catggcattc aaaacagcta
       61 atactgtcac cccaacatct gcaaatactg catctcccat tgatgcaaaa ccaaaagcac
      121 ctaaggcaag taccaaaatt tttatcccaa tagcaaagac aatgttttgt ttgacgattt
      181 tcaatgtttt ctttgctaaa tggattgctt ctgcaatctt cgatggttga tcattcatga
      241 tgactacatc tgctgcttca attgcagcat cgcttcccaa tccacccatt gcgattccta
      301 catctgctct tgctagcacc ggtgcatcgt tgattccatc tccgacaaat gcggtctttt
      361 tattgtttct ttgtaagatt tcctctaacc gctgcacttt atcttcagga agcaattcgc
      421 tatatatctt gtccacgcct acttgcttcc cgatatgatc agcaattttt ttcgagtcgc
      481 ccgtcaacat gactgtgttc ttcacaccag cctgttttaa ttgaaccaat gcgtctacgg
      541 catcttcttt caaggtatct gcaataacaa gataaccact gtaactttga tcgatcgcta
      601 gataaagcaa tgtaccgatt tcttgactta aaggaaaaga gatttcaaat ctttccatca
      661 atttgtgatt acctgctaat acttgtttgc catcaacttc agctaagatc ccttcacctg
      721 caaattcctg gatattagtt gttggtaaaa gcggacctgc gtagccttct aaaacagatt
      781 gagcaatcgg atgtgtagaa aactgttctg cactcgcaat atattgcatg aatttctttg
      841 gatcgatttt tgtatcgatc gtctggatcg aaaagtcacc tttcgttaat gtgccggttt
      901 tatcgaatac cactgtctcg gtttgcgcaa gcaactccaa gtaatttgaa ccttttacta
      961 atacaccgat tttacttgcg cctccgatac cgccaaagaa actaagtggc acggaaatca
     1021 ctaatgcaca tggacatgag atcactaaaa atgtcaatgc tcgatagatc cattcagaaa
     1081 acgccgctcc agaaacaaca agtggaggta aaacagctaa taaaacggcc aatcctacaa
     1141 cgactggtgt gtaatagcgc gcgaatttag taatgaaatt ttcagctggt gcttttttac
     1201 tgctggcatt ttctactaat tctaataatt tgctgatagt agaattttca taagattttt
     1261 ctacttgaac taaaaatggt ttatttttat tgataaatcc gcttaatact gtttcaccaa
     1321 catatacaga tttagggact gattctcctg taagtgctga cgtatcgact aatgattctc
     1381 cttcgatgat cgttccatct aacggtacac gttcaccagg cttcacaagt acaaactgac
     1441 cgatctgcac gtcttcaggg ttcacggtca tcaatccttc cgtcgtctgc acgtgagctg
     1501 tttctggacg gatcgccatc aattcgcgaa tcgattttct tgaatgactg actgccaatc
     1561 cttggaaata ctcgccgatt tgataaaata gcatcactgc tacggcttct ggatattcac
     1621 cgatcaacat agcgccaatc gtagcaacag acataagaaa attctcatca aagatttttc
     1681 ctttgacgat attcatcact gccttcttca ctacatcata tcctatcagc agataaatca
     1741 ataaataccc tatcaagcga attggttcat taaaatcaaa taatagcaat aaagcgatac
     1801 ctgcaaggga aaataaaatc cggaacagcg tatttctctc tttttcattt ccatgttcag
     1861 atactttttc cttatctatt tctgttactt tgacatctgg ttctaattcc ttcactactt
     1921 tttttgtttc ttgttcaatc gtagccaaat gatcttcctc tgcgtcaatc gttagttttc
     1981 cagaagtgaa gtttaccgtt gcttccttga ctccattgat cttctgaact cctttttcga
     2041 ttttcattgc acagttcgca caatctaaac cttctaatct gtaacttttc attttaatga
     2101 cctcctcgcc tcaacatatg aacatctctt cataactaat tataaatgaa tggttattca
     2161 tatgtcaagg tatccgaaag aaaaaatgaa aaacatctgc atcggaaaat ttcttccata
     2221 cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct cttatccaac
     2281 ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct gttgctggca
     2341 atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc cctgcaaaat
     2401 gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag agttctttaa
     2461 tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga cgtaaaagtc
     2521 acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg tatcccacct
     2581 gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg caccgaatgg
     2641 catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc ctatgatcgt
     2701 gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc ctgaaacgat
     2761 cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta cttctttgcc
     2821 aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg ggatacctat
     2881 gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga ttagtccgcc
     2941 gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc tattactgtt
     3001 tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc cagcatcaag
     3061 tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa tgtgtgttgg
     3121 attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac gtccgcttgc
     3181 gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt attcttatca
     3241 tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt aatcagctga
     3301 ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc tcacttttct
     3361 ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct atccagcttt
     3421 gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt gtatatttcg
     3481 aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc agcaatgcgc
     3541 catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct ttacgccagc
     3601 catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg ttgcagattt
     3661 ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc tgcgcagatt
     3721 tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg attccgatca
     3781 aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt acttgcagat
     3841 ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc gttggatatt
     3901 gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca gttgcttctg
     3961 ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt tttttcattt
     4021 gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc ccataagccc
     4081 tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt cccttcaatc
     4141 aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta acgtgccgga
     4201 ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa gcttccgtca
     4261 atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa gacttccagt
     4321 aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc ataactttat
     4381 ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca tgctgaaaat
     4441 acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact agatgctgct
     4501 ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc ttctttgtat
     4561 atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca atttcttttg
     4621 ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa agaactgcga
     4681 gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct cggtcacaag
     4741 tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt gactcggagc
     4801 tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga ccctcgatat
     4861 tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct ctaatactca
     4921 ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc agtttctcgg
     4981 tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt ttcttaatac
     5041 tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa gcttacatct
     5101 gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg gctgttctaa
     5161 ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt tcaacaaacg
     5221 tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga ctgttcgttc
     5281 aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat caagatacca
     5341 tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga aatcatagtc
     5401 tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga tcatatctgc
     5461 acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct tgatggcttt
     5521 tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg tttcatgagg
     5581 tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca gtaattcgat
     5641 tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta agacttcttt
     5701 gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt gtactaagaa
     5761 tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag ccatttctgg
     5821 ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag cacggaaagt
     5881 cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt ataattggcc
     5941 actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat ccgtagtgcc
     6001 ttctggtgca gaaccagtca agatcggcgg atcgattttg acaaaatgat gttgattaaa
     6061 gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat gttgtcttga
     6121 agaacgcagc cataaatgac gatgatccat caagaaatct gttccgtgtt cttttggtgt
     6181 gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga tttcataacc
     6241 aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg tttcttggtg
     6301 tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa ctacaccttg
     6361 gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg atcgcttatt
     6421 tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat cgatgatttg
     6481 aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg attaattttg
     6541 acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg tagcgcgatc
     6601 agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag tcacaagcgc
     6661 tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat acttacacat
     6721 gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct tttcaagaga
     6781 aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt caaaggcgtt
     6841 ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg catattgact
     6901 gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat tgatgatcac
     6961 aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt tagacacgcc
     7021 gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg gagtaaactc
     7081 attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa tatcattttt
     7141 tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc cagtcggatt
     7201 cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt ctagctgttc
     7261 aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa gtccttctgc
     7321 cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga cttcatcttg
     7381 cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt ctgttacgac
     7441 tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa cgattgcctg
     7501 tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat tgcggattgc
     7561 ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc ccacagtcaa
     7621 actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag ctgccaaagt
     7681 gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca ttcctttacc
     7741 ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat caggtaataa
     7801 ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc atcatacatc
     7861 cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt ctgttttgct
     7921 tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc agatttagga
     7981 atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa gtatgtcttc
     8041 tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt ctttgcgatt
     8101 tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta aaaaatcgag
     8161 ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg attaatcgtt
     8221 ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat tccttttcat
     8281 tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt catttttctt
     8341 taagaattca tggatatcct gatagatttc ctccatagct agctctttga tcggtacttt
     8401 tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc ttcgatccaa
     8461 aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg cttggcgtaa
     8521 cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct ctgattctaa
     8581 ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta aacgtgtgac
     8641 tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc tgtcagcgcc
     8701 aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc ggcttccacc
     8761 aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca ttttctgata
     8821 gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat cttcttgttc
     8881 gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc tgacttgatg
     8941 gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat cataaggaga
     9001 ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat agtgtcgatt
     9061 agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag gataccaaac
     9121 agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct gctgcgctgt
     9181 gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc attgatccat
     9241 cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta aatcgatccg
     9301 catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt gtgactgtag
     9361 ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag attccagtgg
     9421 cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc cgttaggttg
     9481 cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt cgcatagcgc
     9541 agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt tcggtaaata
     9601 gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt gtttatggaa
     9661 atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca aatgatgtac
     9721 ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct gtgtttcttg
     9781 tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct tttgcaagaa
     9841 acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa tacctctgtg
     9901 cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat ccaaatcacc
     9961 agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat attgtttttg
    10021 ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt ggcgatagct
    10081 tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg gaatgtcttt
    10141 ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg gtttttcagg
    10201 tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc ctgtagctgc
    10261 ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg cattcatcag
    10321 tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt ttgcttgttt
    10381 ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca cttctttttt
    10441 ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg gtttttcttt
    10501 catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt gtctacttaa
    10561 tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt cgatcagcaa
    10621 aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct catgaataaa
    10681 tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca agaaaatttg
    10741 tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc atcgcatgag
    10801 ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata tggtgtctgc
    10861 caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt ggacgcgttg
    10921 attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg gatgaatatc
    10981 gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa attggatgat
    11041 ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat aagttcttgg
    11101 tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat actacaaagt
    11161 gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca gacacgcaaa
    11221 tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt tgttgcttga
    11281 ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc taaagttttg
    11341 aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt ttctgcagtg
    11401 atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc cataataaaa
    11461 atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata tctggaagat
    11521 gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat agtatatcat
    11581 accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt tttctcagcg
    11641 attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg ttcagttggt
    11701 gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg actacttatc
    11761 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
    11821 tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac gaccaacgag
    11881 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
    11941 atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
    12001 tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
    12061 cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca tttttgtaca
    12121 gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc tgacttgtta
    12181 aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag acctccttat
    12241 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
    12301 ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc
    12361 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
    12421 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
    12481 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
    12541 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
    12601 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggcaaa
    12661 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
    12721 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
    12781 tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc attcaacact
    12841 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
    12901 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
    12961 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
    13021 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
    13081 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
    13141 ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa agaatccttc
    13201 aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
    13261 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
    13321 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
    13381 tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt catggagtcg
    13441 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
    13501 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
    13561 taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
    13621 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
    13681 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
    13741 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
    13801 ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa cgtacccctc
    13861 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
    13921 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
    13981 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
    14041 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
    14101 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
    14161 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat aacgtgcgtt
    14221 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
    14281 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
    14341 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
    14401 gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag atgcgcaaag
    14461 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
    14521 aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat catgggatga
    14581 taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt tatcaaatgt
    14641 tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg caatcatcca
    14701 gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg aagcaaaatt
    14761 cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa cgatcgttaa
    14821 tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta ctggagaaaa
    14881 cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt tgatcttgga
    14941 ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg aaaacacgca
    15001 atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga attataaaga
    15061 aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt tcgtcggtac
    15121 gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg gccatgcgga
    15181 aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct acgacaattg
    15241 gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa ccattgatgt
    15301 aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa aagtatgggt
    15361 acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca aaaaacaagt
    15421 cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta ccgcaacgat
    15481 caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat ccacagaaat
    15541 catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc gacgcgtcgc
    15601 agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt tccatgtgga
    15661 agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt ttcgttatac
    15721 tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt ttttggttct
    15781 ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg atccttcctt
    15841 gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg tcgtgttgga
    15901 aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta tctttgcctt
    15961 ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata ttttcctcat
    16021 tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt ttcttggcgg
    16081 actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca tttatggatt
    16141 gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc attcattagc
    16201 taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt ttgacttttt
    16261 cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc cattgatctt
    16321 gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt atcagttcaa
    16381 ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga tcaatggaga
    16441 aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt attgggcaca
    16501 aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg gagcagacga
    16561 aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa ttcctgaaag
    16621 agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct attcaaagga
    16681 aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca acaattacca
    16741 cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg gcattggggc
    16801 aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg ccatcttgat
    16861 gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt ccgcattctt
    16921 atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat gtcacacctt
    16981 gtttttttct tttattctct tgacgattgg caagaatagt attccttttt tgacggctac
    17041 ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca agcagatcat
    17101 aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa aacaggcaat
    17161 cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa gaaaaggaac
    17221 agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct caaatagtgg
    17281 tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga ataatttccc
    17341 aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg gaagactcaa
    17401 cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg ctccacctat
    17461 cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta aaaagacgat
    17521 cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa aaactttgaa
    17581 gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat gagaaacacc
    17641 atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa atattgcttc
    17701 tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag ctaaaggtgt
    17761 gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct cccgttctgc
    17821 tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca tttgaactgc
    17881 tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc caaacaaggt
    17941 caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat cgatcactaa
    18001 gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa aaagcgcgcc
    18061 gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg tcacgaacgt
    18121 caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa tcgctgtgac
    18181 caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt gtttttccat
    18241 aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc cccagacttt
    18301 agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc acctattcac
    18361 ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt tatttcttta
    18421 cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta cattgatcac
    18481 tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg gctttcgttg
    18541 tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt cccttattaa
    18601 tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa taaaccatga
    18661 attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc aagtcgattt
    18721 cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt
    18781 tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct cgttctgctt
    18841 gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt tctgatggtt
    18901 cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt tgtaccgttt
    18961 ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt tttataaaaa
    19021 aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt ctaaccaaag
    19081 tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga agatcccgga
    19141 ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt tcattcttct
    19201 atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat agtcaactgc
    19261 aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa acaaacggtg
    19321 ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg agaagcaatt
    19381 ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc tcaatatggt
    19441 ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt tgccatctct
    19501 tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga
    19561 atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct
    19621 tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca ttctttacag
    19681 cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat
    19741 agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt
    19801 tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat
    19861 gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta
    19921 cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac
    19981 ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat
    20041 tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg
    20101 ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg
    20161 gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt
    20221 ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt gtgcttcttc
    20281 atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa tcacttctgt
    20341 caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt
    20401 tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc
    20461 tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta ataacagcca
    20521 tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg
    20581 tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat
    20641 ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag
    20701 ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag
    20761 atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct
    20821 tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg
    20881 aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg
    20941 aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg
    21001 taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa
    21061 aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga
    21121 aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg
    21181 tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg
    21241 aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt
    21301 tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa taaggttgtg
    21361 agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt tccacatttt
    21421 tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga taaggttgtt
    21481 tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat ctataacttg
    21541 caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta gttttttaaa
    21601 taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct tgattttttg
    21661 tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga
    21721 agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag
    21781 aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc
    21841 ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga
    21901 atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat
    21961 ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc
    22021 ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg
    22081 ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa
    22141 caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc
    22201 ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt
    22261 acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga tttaggctca
    22321 ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg atacatctca
    22381 acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat cgtctctcct
    22441 gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc
    22501 ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg
    22561 aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt cactccaaga
    22621 aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa acaaatcaaa
    22681 caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca gaagcggaaa
    22741 taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt actcttcctc
    22801 ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc ctgcacgaac
    22861 aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca tcggaagatt
    22921 catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa tgatatttgc
    22981 aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat ctgtgacagt
    23041 gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta acgtgtagat
    23101 atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac aaaattttcc
    23161 gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc gaaatcacac
    23221 ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga gtaggagtca
    23281 tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt aaaattttag
    23341 gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc gttggtgccc
    23401 atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat acccctaata
    23461 aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact aaggaaattc
    23521 cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg atctgagcaa
    23581 aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc gccaaagccc
    23641 caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca aaggaaccca
    23701 tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct ttttcttcca
    23761 ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta aaaaattgcg
    23821 tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt tttcttagtt
    23881 gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc caacctaatt
    23941 gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt aatttgtaat
    24001 tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact agctaatggt
    24061 tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag accaatcaat
    24121 gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac aacgcttgcc
    24181 acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc cgtgatcgta
    24241 aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag aagaatggca
    24301 aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag ccattttaga
    24361 ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc taaagctaaa
    24421 aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa attgcgttcg
    24481 attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa gtatgtgtta
    24541 gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac tgcaatagca
    24601 gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc taaaatcgtc
    24661 ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa aactgtcaac
    24721 tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa acctgcatat
    24781 aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc taaaatttga
    24841 taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat ggatcattcc
    24901 tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac catttgagca
    24961 attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc atctaccaca
    25021 ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc cacatttact
    25081 tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt ttttgctacg
    25141 atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc tttttcgatt
    25201 ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact tttttcaatt
    25261 gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag tttcgttgtc
    25321 caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc aataattgcc
    25381 atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact gtgacatcat
    25441 caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat atttcatttt
    25501 taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata tttaagttca
    25561 tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag ggtcgatcca
    25621 gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg cgagaatctc
    25681 ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg cgtcacctga
    25741 accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac actgagcaac
    25801 ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc cttcaaatat
    25861 tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga tacgttggcc
    25921 aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct ccttaccttc
    25981 caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc cttttttatc
    26041 agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta aactacctga
    26101 gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa atgtctgttg
    26161 ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg aactatgcag
    26221 gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca tgatccctaa
    26281 ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac cagtagctat
    26341 cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc ctcccgctgt
    26401 tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa ttgtatacgc
    26461 gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac ctttttgctt
    26521 tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa agtctcgatg
    26581 ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt ttcgacgtat
    26641 gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa ggtggaaccc
    26701 gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc cccactcaaa
    26761 aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc agcctcttct
    26821 ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt gactggtact
    26881 tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat catctcaata
    26941 actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat ttgtgcgtca
    27001 taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact acctaaacgt
    27061 tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc gtagtaaagt
    27121 aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca atccgctaat
    27181 aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata agctaacaat
    27241 aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg atccaataaa
    27301 atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc actgatttgg
    27361 tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc acgctgatcg
    27421 atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc cctatttttt
    27481 tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga cgcccttgac
    27541 tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta ctttgattga
    27601 agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat aggcatcggg
    27661 caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa tagtacccgg
    27721 ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct ctaaaatatc
    27781 taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga gtgtctcgct
    27841 atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa ttgtagcaac
    27901 tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac ctgccaaact
    27961 tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt gaatttcttg
    28021 atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc tttctttttt
    28081 ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg taaaacttcc
    28141 tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat aattctgttt
    28201 atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg tcaaatgaaa
    28261 caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg gactgttgcc
    28321 actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt cttttggtga
    28381 agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt ctccatgttc
    28441 atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac ccgtaaaaat
    28501 aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata gagctaaatt
    28561 ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt gttgttttat
    28621 ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata gctcaaacat
    28681 gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa ctggtccgct
    28741 cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt tatcctcgtc
    28801 agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt tttttgcacg
    28861 atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc ctgtgaaaac
    28921 acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa tagtttcgcc
    28981 ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa tcttttaagt
    29041 ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt attagcttcc
    29101 atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg catttcttct
    29161 gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga gacataggta
    29221 ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa agccagacgt
    29281 acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa cagcttatca
    29341 tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg ataatacaat
    29401 gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt tataaccaca
    29461 acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa aaaagctatt
    29521 ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa aataccagtt
    29581 accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa atggtctaac
    29641 ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat cgttttatta
    29701 cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt tattattagt
    29761 tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa cattttttta
    29821 aataactaat tattttttga atctgtcact cagacaatcc tttctcttac ttgttaacat
    29881 aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt tgttttcctt
    29941 cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt gctactccct
    30001 tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat tgttttgcaa
    30061 aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa ataaacggtt
    30121 gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga ctataacaag
    30181 tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact ttcaatacat
    30241 ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta tgcggtttta
    30301 cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa aggagctcta
    30361 aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata actatttgtt
    30421 tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat ttacaagcat
    30481 ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct ggtagtcggc
    30541 ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca cattctttct
    30601 ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg gaagcatacg
    30661 gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat gtagcttctt
    30721 tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc gcgctaataa
    30781 ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc attttcttca
    30841 tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg attttgcagc
    30901 tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag acaataattt
    30961 caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa aatcaactga
    31021 ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa tcaaactagg
    31081 ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta ttcctgtagt
    31141 accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat gagcgattcc
    31201 taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat ctaatggctg
    31261 ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc tcttccttct
    31321 tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc agttactctt
    31381 ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag aatcgctgta
    31441 acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg tggggctggt
    31501 acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa catcatcgtt
    31561 ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt aatatgagtt
    31621 gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa atatttacca
    31681 aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc tgacgcacca
    31741 gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata tgccgcctta
    31801 ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc aagaacaagt
    31861 ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat agccttcatt
    31921 ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac tggatataag
    31981 acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt ttgcaaaaat
    32041 aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc agtaccgcca
    32101 ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt cgcgatccct
    32161 tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc gtcaactgct
    32221 tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga atagattccc
    32281 caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg caacatatga
    32341 gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc acttatcgct
    32401 tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt tcctgccaat
    32461 gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac atcaatgact
    32521 cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat gacagcatct
    32581 gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc atgggtctcc
    32641 actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg cgccatgtag
    32701 gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt tcccatcttt
    32761 cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc aaaaaatcag
    32821 tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt aacgtttcat
    32881 caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca tcacctgcac
    32941 atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg tctttcgtaa
    33001 aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct gactctacta
    33061 gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg aaccaagaag
    33121 aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa tccatgactt
    33181 cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa acctttggag
    33241 acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt aatgctctta
    33301 tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga ttcactaatt
    33361 tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc ataccaggca
    33421 cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata tactttcctg
    33481 tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt aatactagtg
    33541 acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg tttaccataa
    33601 aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc gtgcatgtgt
    33661 agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa tagaaaatgt
    33721 tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc gtgctttttt
    33781 taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg aaacagagca
    33841 gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt ccatcgtgaa
    33901 actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga aaaagaaagg
    33961 ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca agaacatcaa
    34021 tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa aaaatgaaca
    34081 agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg caatacaaag
    34141 aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa tgattggcca
    34201 agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata ctgatccaga
    34261 aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt ttgtttcttt
    34321 gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta tttttcaatt
    34381 ttttaaat
//
LOCUS       DAYYAO010000025        31896 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000025 DAYYAO010000000
VERSION     DAYYAO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.026"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACHARI_001074"
     CDS             complement(26..601)
                     /locus_tag="ACHARI_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8442136.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACHARI_001075"
     CDS             complement(606..1580)
                     /locus_tag="ACHARI_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8442137.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACHARI_001076"
     CDS             1863..2831
                     /locus_tag="ACHARI_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8442138.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACHARI_001077"
     CDS             2928..3221
                     /locus_tag="ACHARI_001077"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8442139.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACHARI_001078"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACHARI_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8442140.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACHARI_001079"
     CDS             3678..4433
                     /locus_tag="ACHARI_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8442141.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACHARI_001080"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACHARI_001080"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8442142.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACHARI_001081"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACHARI_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8442143.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACHARI_001082"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACHARI_001082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8442144.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACHARI_001083"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACHARI_001083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8442145.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACHARI_001084"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACHARI_001084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8442146.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACHARI_001085"
     CDS             9170..9712
                     /locus_tag="ACHARI_001085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8442147.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACHARI_001086"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACHARI_001086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8442148.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACHARI_001087"
     CDS             11305..12207
                     /locus_tag="ACHARI_001087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8442149.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACHARI_001088"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACHARI_001088"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8442150.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLTGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACHARI_001089"
     CDS             13650..14072
                     /locus_tag="ACHARI_001089"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8442151.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACHARI_001090"
     CDS             14541..14771
                     /locus_tag="ACHARI_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8442152.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACHARI_001091"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACHARI_001091"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8442153.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACHARI_001092"
     CDS             16182..16358
                     /locus_tag="ACHARI_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8442154.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACHARI_001093"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACHARI_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8442155.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACHARI_001094"
     CDS             16853..17320
                     /locus_tag="ACHARI_001094"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8442156.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACHARI_001095"
     CDS             17405..18289
                     /locus_tag="ACHARI_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8442157.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACHARI_001096"
     CDS             18403..18639
                     /locus_tag="ACHARI_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8442158.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACHARI_001097"
     tRNA            18727..18807
                     /locus_tag="ACHARI_001097"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACHARI_001098"
     tRNA            18814..18885
                     /locus_tag="ACHARI_001098"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACHARI_001099"
     CDS             19011..19181
                     /locus_tag="ACHARI_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442159.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACHARI_001100"
     CDS             19401..20108
                     /locus_tag="ACHARI_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8442160.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACHARI_001101"
     CDS             complement(20167..20811)
                     /locus_tag="ACHARI_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8442161.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACHARI_001102"
     CDS             20938..21225
                     /locus_tag="ACHARI_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442162.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACHARI_001103"
     CDS             21333..21653
                     /locus_tag="ACHARI_001103"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8442163.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACHARI_001104"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACHARI_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23763
                     /locus_tag="ACHARI_001105"
     CDS             23044..23763
                     /locus_tag="ACHARI_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8442164.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23891..24442
                     /gene="raiA"
                     /locus_tag="ACHARI_001106"
     CDS             23891..24442
                     /gene="raiA"
                     /locus_tag="ACHARI_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8442165.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24664..27198
                     /gene="secA"
                     /locus_tag="ACHARI_001107"
     CDS             24664..27198
                     /gene="secA"
                     /locus_tag="ACHARI_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8442166.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27324..28425
                     /gene="prfB"
                     /locus_tag="ACHARI_001108"
     CDS             join(27324..27395,27397..28425)
                     /gene="prfB"
                     /locus_tag="ACHARI_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8442167.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28530..29216
                     /gene="ftsE"
                     /locus_tag="ACHARI_001109"
     CDS             28530..29216
                     /gene="ftsE"
                     /locus_tag="ACHARI_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8442168.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29209..30093
                     /gene="ftsX"
                     /locus_tag="ACHARI_001110"
     CDS             29209..30093
                     /gene="ftsX"
                     /locus_tag="ACHARI_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8442169.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            30245..31693
                     /gene="cls"
                     /locus_tag="ACHARI_001111"
     CDS             30245..31693
                     /gene="cls"
                     /locus_tag="ACHARI_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8442170.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10608 a         5564 c         6908 g         8816 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtctta caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt ggctagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaaa
    23521 attccttttc agatgctgct caaaagagag caacatctgc ttgctcaagc aaaaaacacg
    23581 cgaaaggaaa gattggagat gcctcagcct tttgagcttg ccgtacctct ttctaagata
    23641 caacataaaa aaatattgat tattgacgat gtctatacaa caggacagac gctttttcat
    23701 gcagcagatt gcttgcagaa cgcacagccg aaaatcatcc gaaccatttc tttggcaaga
    23761 tgaatcagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
    23821 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
    23881 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
    23941 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
    24001 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
    24061 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
    24121 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
    24181 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct gctgctttat tcaatgaaga
    24241 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
    24301 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
    24361 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
    24421 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc
    24481 tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa
    24541 attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa
    24601 aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa
    24661 cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta
    24721 gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa
    24781 caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat
    24841 caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta
    24901 tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa
    24961 atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg
    25021 actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg
    25081 gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa
    25141 tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa
    25201 ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt
    25261 ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg
    25321 ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac
    25381 ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc
    25441 agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac
    25501 gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc
    25561 atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc
    25621 actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct
    25681 aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac
    25741 tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa
    25801 gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc
    25861 cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg
    25921 caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt
    25981 gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg
    26041 aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct
    26101 gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta
    26161 agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat
    26221 aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg
    26281 tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat
    26341 cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta
    26401 gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa
    26461 tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc
    26521 atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt
    26581 gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat
    26641 tttgccggca gtactcttgt ccatgaagat agcattactc ttgcagatat cgaaggaaaa
    26701 acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa
    26761 gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt
    26821 gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc
    26881 ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag
    26941 atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct
    27001 gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa
    27061 gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaat
    27121 aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat
    27181 ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga
    27241 attcgtctca ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa
    27301 gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa
    27361 tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac
    27421 atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca
    27481 caaaaagtga tcagcgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt
    27541 gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca
    27601 gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat
    27661 gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat
    27721 ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca
    27781 cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa
    27841 gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa
    27901 tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt
    27961 catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa
    28021 atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac
    28081 atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct
    28141 agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct
    28201 aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa
    28261 caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg
    28321 gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac
    28381 cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa
    28441 ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta
    28501 aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt
    28561 actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg
    28621 tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag
    28681 aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa
    28741 accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat
    28801 taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac
    28861 ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag
    28921 ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg
    28981 caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag
    29041 aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg
    29101 tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg
    29161 aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc
    29221 tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt
    29281 gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc
    29341 atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta
    29401 gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat
    29461 catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa
    29521 atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta
    29581 gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag
    29641 aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt
    29701 gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc
    29761 atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt
    29821 ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc
    29881 ggtctgatcg gtgcaattct tccaattttg atcatcacat ttggttatgc atggtttttc
    29941 caattgatca atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc
    30001 gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg
    30061 atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag
    30121 ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct
    30181 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga
    30241 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
    30301 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
    30361 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
    30421 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
    30481 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
    30541 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
    30601 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
    30661 caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
    30721 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
    30781 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
    30841 aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
    30901 ttatcgcaat catcgtaaaa tcgtagttat tgatggggaa atcggatata caggtgggtt
    30961 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
    31021 tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa
    31081 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcacactac
    31141 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
    31201 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
    31261 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
    31321 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
    31381 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
    31441 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
    31501 tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc
    31561 ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
    31621 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
    31681 gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc
    31741 cttttttaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
    31801 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
    31861 gctattaaaa gagacacatt tcaaagccag tatagc
//
LOCUS       DAYYAO010000026        31421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000026 DAYYAO010000000
VERSION     DAYYAO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.027"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..148)
                     /locus_tag="ACHARI_001112"
     CDS             complement(<1..148)
                     /locus_tag="ACHARI_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8442171.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYH"
     gene            complement(167..1207)
                     /locus_tag="ACHARI_001113"
     CDS             complement(167..1207)
                     /locus_tag="ACHARI_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8442172.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            complement(1223..2614)
                     /locus_tag="ACHARI_001114"
     CDS             complement(1223..2614)
                     /locus_tag="ACHARI_001114"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8442173.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            2992..3975
                     /locus_tag="ACHARI_001115"
     CDS             2992..3975
                     /locus_tag="ACHARI_001115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8442174.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(4343..6481)
                     /locus_tag="ACHARI_001116"
     CDS             complement(4343..6481)
                     /locus_tag="ACHARI_001116"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8442175.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(6520..8106)
                     /locus_tag="ACHARI_001117"
     CDS             complement(6520..8106)
                     /locus_tag="ACHARI_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8442176.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(8096..9316)
                     /locus_tag="ACHARI_001118"
     CDS             complement(8096..9316)
                     /locus_tag="ACHARI_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442177.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(9328..10134)
                     /locus_tag="ACHARI_001119"
     CDS             complement(9328..10134)
                     /locus_tag="ACHARI_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442178.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(10344..11624)
                     /locus_tag="ACHARI_001120"
     CDS             complement(10344..11624)
                     /locus_tag="ACHARI_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8442179.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(11668..13701)
                     /locus_tag="ACHARI_001121"
     CDS             complement(11668..13701)
                     /locus_tag="ACHARI_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442180.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(13735..14586)
                     /gene="rfbD"
                     /locus_tag="ACHARI_001122"
     CDS             complement(13735..14586)
                     /gene="rfbD"
                     /locus_tag="ACHARI_001122"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8442181.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(14684..15712)
                     /gene="rfbB"
                     /locus_tag="ACHARI_001123"
     CDS             complement(14684..15712)
                     /gene="rfbB"
                     /locus_tag="ACHARI_001123"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8442182.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(15734..16306)
                     /gene="rfbC"
                     /locus_tag="ACHARI_001124"
     CDS             complement(15734..16306)
                     /gene="rfbC"
                     /locus_tag="ACHARI_001124"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8442183.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(16320..17186)
                     /gene="rfbA"
                     /locus_tag="ACHARI_001125"
     CDS             complement(16320..17186)
                     /gene="rfbA"
                     /locus_tag="ACHARI_001125"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8442184.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(17286..17993)
                     /locus_tag="ACHARI_001126"
     CDS             complement(17286..17993)
                     /locus_tag="ACHARI_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8442185.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(17998..18825)
                     /locus_tag="ACHARI_001127"
     CDS             complement(17998..18825)
                     /locus_tag="ACHARI_001127"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8442186.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(18825..19625)
                     /locus_tag="ACHARI_001128"
     CDS             complement(18825..19625)
                     /locus_tag="ACHARI_001128"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8442187.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(19618..20757)
                     /locus_tag="ACHARI_001129"
     CDS             complement(19618..20757)
                     /locus_tag="ACHARI_001129"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8442188.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(20962..22155)
                     /locus_tag="ACHARI_001130"
     CDS             complement(20962..22155)
                     /locus_tag="ACHARI_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8442189.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(22234..22440)
                     /locus_tag="ACHARI_001131"
     CDS             complement(22234..22440)
                     /locus_tag="ACHARI_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8442190.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(22433..23575)
                     /locus_tag="ACHARI_001132"
     CDS             complement(22433..23575)
                     /locus_tag="ACHARI_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8442191.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            24087..24722
                     /locus_tag="ACHARI_001133"
     CDS             24087..24722
                     /locus_tag="ACHARI_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8442192.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            24858..24950
                     /locus_tag="ACHARI_001134"
                     /pseudo
     CDS             24858..24950
                     /locus_tag="ACHARI_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            25064..25699
                     /locus_tag="ACHARI_001135"
     CDS             25064..25699
                     /locus_tag="ACHARI_001135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8442193.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            25806..27044
                     /gene="mef(H)"
                     /locus_tag="ACHARI_001136"
                     /gene_synonym="mefH"
     CDS             25806..27044
                     /gene="mef(H)"
                     /locus_tag="ACHARI_001136"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8442194.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            27271..27690
                     /locus_tag="ACHARI_001137"
     CDS             27271..27690
                     /locus_tag="ACHARI_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8442195.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            27695..27934
                     /locus_tag="ACHARI_001138"
     CDS             27695..27934
                     /locus_tag="ACHARI_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8442196.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(28159..29082)
                     /locus_tag="ACHARI_001139"
     CDS             complement(28159..29082)
                     /locus_tag="ACHARI_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8442197.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(29114..29878)
                     /gene="map"
                     /locus_tag="ACHARI_001140"
     CDS             complement(29114..29878)
                     /gene="map"
                     /locus_tag="ACHARI_001140"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8442198.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            30036..30476
                     /locus_tag="ACHARI_001141"
     CDS             30036..30476
                     /locus_tag="ACHARI_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8442199.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(30654..31223)
                     /locus_tag="ACHARI_001142"
     CDS             complement(30654..31223)
                     /locus_tag="ACHARI_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8442200.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
BASE COUNT         9621 a         6220 c         5391 g        10189 t
ORIGIN      
        1 aatgatagtc ttgaacatct gcataatctt cataaatcaa aaaatcaaat tgatagtttg
       61 tacgatcaat tttacgatat aaattcatca tgaaagactc tgctccacct aaacccattc
      121 gtccctgaaa atgcaaaatt cttttcatta tattttcctc cattaactaa tatactcgct
      181 tcaaagcagc cattaaccct ggcattttca cttcaacgaa gcttccaact tttacagcag
      241 ataaaaatag tggagctggc atattgtaaa gcaactttgt tatttttttt gctgaagaat
      301 ccgtcttagc aatttgcttg gcttctcgga tcactggatc actcaatatc tgctttgctt
      361 cgtctttctt ctcttttgaa gttcttgcag aggtactata aatagaaaaa ataactgaag
      421 ttgtcccttt taaaagaagc gccgccatac cgtctttatt aatatcgtct gttacagtat
      481 ttgctaatga atagaattct ttaaatgttt cgaacttgtt aggtcggtat tgattagtga
      541 tccccttccc accttcaaat tcataaatat attctacttt tgggttcatg atcacttgat
      601 gacagtattc ataataatgt aagttaaata ttcgatcctc acaactatta taagttttaa
      661 atagaatttg gttatttttc agaatatcgc tacgatagca tttgttccat acaacataca
      721 tcaactgttc attcattaac tgatacatgt cttttataaa ctcttcgtga gttagatagt
      781 gacattctgt aactaagttt tctttttcgg aaacgattct tccctcgtcc attgtacgaa
      841 aaacaaacga actaacaaca agatccgctt ttggactctt ttctagcaaa tgatcataat
      901 ctgacaagat cgtttttact ggcgtgtcat ctgcatcgaa gaataagaga tattctcctt
      961 ttgactgtct tattccttcg tttcttgcta atccgggccc ttgattggtc gtagtaatca
     1021 attcaaaacg ttcatcattt tcaatcagtt cttgaatgat ttcctgtgtt tgatctgttg
     1081 atccatcatt tactaaaacg acttcgtact cttcataatc caattctttt agaatatcaa
     1141 tcgttcgacc aatagttgct tctgcattgt atactggtac gattatgctg aatttcggat
     1201 tgttcatttt tacgcctccc aactagcgaa agattttttc attcggaaaa gcaatttctg
     1261 ccaatgagat cacattttcg tcttcttcaa gtccttgaga ggacatttta tcaaatagga
     1321 tcttgatggt ttgcagcaag atctttaaat caagcatcaa tgaatatttt ttgatataaa
     1381 gtaaatcaaa acgtaattta ctgttgtaat cagagacata ttttccatag acttgtgcat
     1441 acccagtgat accagcacga acattatgcc gtaagtagta ataagggttt tctgcattga
     1501 actgatcgac gaagaaagga cgttcaggac gtggtcctac gatcgacata tcgcctttca
     1561 gcacattgaa gatttgtggt aattcatcaa agcggacagc tcgaataaat ttacctacag
     1621 gcgttactcg tgcatcattg ctcttagcta aaactggtcc agattttgct tcggctgtgg
     1681 cggtcatcga gcggaattta tagattgaaa attccttttg attttttgtg atccgcactt
     1741 gtttatagat gattggtccc ggtgatgtca ctttgactaa aattgctgtg aataacataa
     1801 atggtgaagc aataactaat aaaacaagtg aaacgatgat atcaaacaaa cgtttgaata
     1861 gttcatattc aggttggatt ctaaatccag atgcttcaat aatactttca tcttcgaaat
     1921 tcataatatt tggattgatc aatgtcaagt tttcaaatgt tgtattcaaa aacaattttt
     1981 tttcattttt agtaactaat tgatagattt tgattttttc agtttcttcg atatgagaag
     2041 caagatagac aatatcgatg tctttaatca tatttttgat gtttgcataa tagttatcaa
     2101 gaatgactgc ttcgacttta tgtttgttgt ttttctttga accaaaattt tggattgctg
     2161 gtgcgacatc cttttctaac ccaataatga cgaccttttt atctcgactt acgcgtaaat
     2221 aaagtttata tacgagaata cgatagatac ttaagaaaag cgtaccgaga acaaatgaaa
     2281 gtaagataac aagacgtgga aaagcaaacc agcggcccgc aaaagtcact accatgatcc
     2341 ctagtgtcat caatacctga ccgatgactg tgacaaacac gatgtcactg atcattcgat
     2401 tataaaaaac atatgcaccc aacaaaatgt tcaacgcaat aaaaatcact gaaataataa
     2461 ttgctgagtg ttggaaagtt tctaaatttc gtaatgggat ctctccacga aacttcagta
     2521 agaaactact atatattgct gcattgtata caataacatc cattaatata atgaatatcc
     2581 gtcgtgcagc actccattct ccatttttat tcatatatac actcctaatc acaaaataat
     2641 atctctatta tcttaccata gtcaccttta aaaacctagt ttataagcaa attgtaatgg
     2701 aatttaaaaa tgtctaataa aaagctttat acacctctaa tcaagacaag gactctaaat
     2761 tacaaatctc cagatgaatc gattttcact aatgttggta aattaaagaa gtcgaagtat
     2821 ttgctgcttt ttttatccac tcataaagtg aacctttata tttttcagag aatccatctt
     2881 cccaaagcgc tgtctagtcc ataagaatac ctgtattacg tgaagaatcg aaaaaattga
     2941 aatcgatttt taatgacatt atgcttatag aagaaagtga ggtaatatct tatgaataaa
     3001 caagagttgc atgattttat tggttttcga caaccaaata tatgtcaaat tgtcgcatat
     3061 aaggatgggg tagaagttta tacagatgaa tggaatggtt ataaaaaaga cgatacttgt
     3121 catatcatgt cagcaacgaa aagtattgta tctttgctga ttggcattgc tttagagcaa
     3181 ggattaatca aaagtatcga tgataaagta ttagattatt ttccagagta taaagtgaaa
     3241 cgcggggaga aaactattta cgctattaca atcaagcact tgctaacaat gaaagcaccc
     3301 tacaaatgta aaggcgatcc atggacaaaa gtctgttcaa gtgaagattg gacaaaaaca
     3361 tcactagatt tattgggagg aagaaaaggt ataaccggcg aattcaacta tcaaactgtc
     3421 tgtttacaca tattaactgg cttgttgtat cagacaagta agctaacaac tgttgatttt
     3481 gccaataagt atctatttga tcctataggg ataccaaacc atgtcaatta ttatgctgaa
     3541 acagctgaag aacataagca gtttacaatg gataagtctc ctaaaaccaa tgtctggttt
     3601 tgtgatccac taaatatcgg gacggcagga tatggtttgt gtttatcggc aagtgatctg
     3661 gcaaaaattg gtgtattatg cctaaataaa gggatgtata aaggaaaaca aatcgtacca
     3721 cccatgtgga taaacgagat gttcaaacct agaaccagtg ctttagaaga cttacaaaat
     3781 atgagatatg gctatttatg gtgggtatta gattctgaga acgaaatata ttcagcaatt
     3841 ggtaatagtg gaaatgttct ttatattgac gctaagaaca atctggttat ctccatcact
     3901 tcttatttta aacccatgat aattgataga gtggcttttg tagaggaata ccttaaacca
     3961 tttattttaa aatagcctat attaaaataa aatatcgatt ctttgacagc tagggttcgt
     4021 gaaactttgg ctatgatttt gtatctattg gcgctctgag catgaataag agcgtgggac
     4081 aaaagtaaga aacacttttg tctcattctt taaaaacgaa taaacggtgg gagcagaagc
     4141 aacttcttct taaataagcc gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
     4201 ttcttatttc tcggagttaa acacttctgt cccaacctcg acttattttt ttgagtcggc
     4261 accttctgcc tttttttgac aaaaaactat aaaaagccgt tatgtgcgag cgaatcacaa
     4321 cataacagct ttttattata gtttaatctg gtctcaacga aaagtcgccg attccttctt
     4381 ttgttaaatt atcattgtaa aatggatcat gcgcaatata tgctggccat ttatcttgca
     4441 tttttttgat ttcaccagca aaacgtcttt gtttttctgg agtatcttca taacctcttg
     4501 atttagattc gaaatggtac aattctgctt gatgtgcata gacattgtaa cgacccagtt
     4561 cataaacttt caagcacaag tcaacatcgt tgaatgctac ttctaatgtt tcatcaaacc
     4621 cgttaacagc attgaagtca gctgccttga ccatcataca agctgcagtg actgctaagt
     4681 aattgacgtc aatgaccaat cggccgaaat aaccacaatg tgttcgatca tagttattca
     4741 atgcatgccc tgcaacacca ccgattccta ataatactcc tgcatgctgc gttgtatcat
     4801 ctggataaaa cagttttgct cctacacagc cgattcgatc aaactgtgca taagaaacca
     4861 tcaccgtcat ccaatctggc tcaatcactt ctgtatcgtt attcaagaat aaaaagtatt
     4921 tgccgtttgc tttttctgct gctaaattgt taattcgaga ataattgaat ggaatgtcaa
     4981 tcaattctac gataaagcgg tctttcagct gatgcttgta ttctgcaaat aattcttgca
     5041 tttttggatc tgtacttccg ttatccgcaa taatgatttc ataatttgaa taagacgttt
     5101 tttcgatgat cgaatccaca caagttttta gatcttcata gccattcttc gttggaatga
     5161 tcacactgac caaatcctct tgcaagacgt cataagcaat ctcataaaat ccagaaattc
     5221 tgccaggacg tacgcttcct ttgatgtttc ttctagacag ggcatctgtc aatgctttca
     5281 ctccggaatc atagatataa cttttggctt cgccattact tgcagttgat cctgggatcg
     5341 tccgccaatg atacaagact cggtcaatgt ggtaaatatg atctgcaggg atttgttctg
     5401 tcacacgaag gaccaaatca taatcttgag aaccttcata gcctttacgg aaaccgccaa
     5461 gttctttaac aatgcttgtt cgatatacgc ctaagtgaga gatataatta tttcccatca
     5521 atgtatcagg cgaccagtct gctttgaaat gcggatcaaa gcgattgcca tctgcatcga
     5581 ttttatcttc gtcactgtag atcacatcca gttctggatg tacgtttagc acttttgcca
     5641 cttcatataa tgcaaatggc ggtaattcat catcattatc taagagtgct ataaattctc
     5701 cttctgctat ttccaatgca gaattagttg ccagactgat gtgtccattt tcttttcgga
     5761 aaacgacttt gattctatca tcttttgctt gataggattc cagacacttt cggatagctg
     5821 gatcagtaga agcatcatca ctgatacata gttcccaatg atcatacgtt tgatccagta
     5881 ccgaatcaat gcatttttcg agccatttga tttcaacgtt atagacaggc ataagtatgg
     5941 aaatcaatgg acggtacgca aatccttgtg cttctttcct ttgctctttc aaatctggtt
     6001 tctcatgacg actgatccac tctgcatagt cgatcgatcc tggcttcaat tccaatttga
     6061 cccgacggat cgcttttttt acgccatgag tccttgcata atttatccct tttttcagta
     6121 atcttttttt tctttcccat gaagattcaa tgccgtcatc ataagggtat ttaccgttta
     6181 atttcacagc tatgccattt tgatgtctcg ctgtttggaa gtctaagatg gcttttcctc
     6241 tcattttccc tgataaacgt accttaaaac cgctttgggt cttaacatca agattatata
     6301 aatgattgat atccaatctt acgacatgtg ttgcttcagc aatcacattt tctttttcta
     6361 cctttatcgt agggatttct ttggtcactt catcaattgc ccagcccaca ataagcaaat
     6421 cgccagtggc ccgttcacga gtgatttgat cgataaatac ggcaatttct tttttttgca
     6481 cgattcgttt actccttatc tttataagtt aatcattatt tacatttcaa tgatccatga
     6541 ttggccattt tgagctactg cttccactaa gaatgaacgt tcttgctcgc aaaggattcg
     6601 taactcaaat cccgcttctg ttccttttgc gagctgttct gcttgattga cttcttctcg
     6661 tgaaacagga attgcttcga aaaatggcga ttggtcagct gataacttat aggacaaggg
     6721 ctggcgatcc gaattagcga ttccccatcc gatgattttt aatatttttg tttctgcatc
     6781 ccagattttc tcgtcaatac atatagaaag ttctttttct tggatttttt tcgaagtttc
     6841 tttcttacgt cgtcctagtc tatgttttat tgtccattca tcactttcca acaatgtgca
     6901 atagcgattt gtggttgttt ttaacgtatg gtctagtctt gtattttcct caagaagatg
     6961 atgattttct ctttctaatt cgtcattttt ctcagaaagc tgctttgtga cttcagccat
     7021 gggacgaatc gtttccaaca attctttcat tgttggtgtc aattccccga taaagcgata
     7081 gtgacaatgg atagtcactt ctttcccaga aatatccgtt agaacgaact ctggtaattc
     7141 tttcccatca aacaaataac aatcatttac tgttttgact acagcattcg aatcgataaa
     7201 tgttagtttt tctccggcag cttctgcgct gaattcgata aagctaggtt gttgcgcgaa
     7261 gcgaaacacc attctttctg ttgtttctga tacggggaaa cttagtgtct cattttcccc
     7321 tgtgaaatta ttgaatggta tttgttggat tgtttcatct tgtttcgttt gctgggttac
     7381 ttctaacact tttacataat tagaattttg tggctctgca atggaacttt gtgctactgg
     7441 atgtttcatc aatgagaaaa agtattggta gacttcacca aatggtcgca ttttgaaatc
     7501 atagcgtaca gcttccggca attcttcata agttgatttt aattctgtat cacctacagc
     7561 taagtacaaa taatcttcaa tattaatgaa taatcccgct ttttcaaaaa ctttttggaa
     7621 gccatcatga gtataaaaag aattatgggt ttcatctagg agaccataag aagcccacgg
     7681 caattggtta ttgaacaaat cgatcatgac agaattatgc gccaagttag ggaatgtaat
     7741 caggatctct ccttcttcat tcaaaaattc tctgactttc ttcaatgttt ttccaggatc
     7801 taccaaatgt tctaaaacat ccgcaaacaa gacgtaatca aatgtctgtc cagcataata
     7861 gtttgcccac tcaaaacttt cgatatcacc ataaaatcca tcttgagcaa attcgctgac
     7921 aaaatcgaac aattccttat ctaactcaac aatgctcact tcacattgtt ttgctccgat
     7981 taaatgtttc gttaatcggc cgtttccggg accaaactct aaaactttac ttcctggttt
     8041 gatctgtgcg gcaatttttc ccacactgct ttgttcatct aggtccattt caaaatcata
     8101 tttcattgct atgagtccct tcttcccatt tatggtctaa gaccacgatg ccttctccga
     8161 tataagaacc gctgacaaac aagttcatgt actttgtctt atataccaaa ggaaccgttg
     8221 cattttcttc aaagaccgca acgtcaaaat aatattcacc agctaacaaa cctaactttt
     8281 catactccag ataaaagata ttctctcctt ttttccaagg aatcttgata ccatctagta
     8341 gagtattaag tccgcataca taaaaattgt cgactgttcg tagcgcaatt cctaaaaccg
     8401 gatttttgat tgtatcatct tttacttcat aagttacttt aacgtatagt ttttctcctt
     8461 gttccagcat atccaccgga tttttgtctt tatccaacaa ttccgctttc gtcacgtcga
     8521 cgatatctgg agcggcgaac cgttctactt ggtttttatt ccgatccact gttttaactg
     8581 attttttctt taaaaagttt tcataattcg tcgttacttc catcgtatcg ccatattcga
     8641 cgacttctcc gtttttcaac caataggaac gatcacaaaa acgacgaatc gaactgatat
     8701 catgagaaac gaataaaatc gtctttcccg agttcttgat ttctgtaaat ttttccatac
     8761 attttagttg aaattcaaga tcgcctactg ccaatgcttc atccacaatc aaaatatcgg
     8821 gattcacatt gattgctaca gcaaaagcta gacgaacgaa catcccgctt gaataagttt
     8881 ttacaggttg ataaatatgg tctccgatgt cagcaaattc aatgatatca tcgaccattt
     8941 catcgacttc ttctcttgaa aatcctagaa ccattccatt taaatagata ttttcatagc
     9001 cagaatactc aggattgaat cctgatccta gttctagcaa agctgagatt tttccttcga
     9061 tttccatcgt gccggatgtt ggtgtcagta ctcccgtaat gattttcaac attgtagatt
     9121 ttcccgatcc gttttctccc acgaaaccga tcatctcacc tttttttatt tctacattca
     9181 cgtttttcaa cgcataaaat acatcatgat aggatttctt aaaaggattg agggcttcac
     9241 gaaatcgatc cgaaggtttt gcgtacatat tataagattt tgtgatatct tttagtctaa
     9301 ttgcatattc tgtcatagct attctcctta cagcacatct gaaaaatgtg gtttcaatct
     9361 acggaacact gcagagccga ttacgaacat tactaacgtg aaaacccaga aatacaaagt
     9421 atatgttcca tgctcccaga accaagcttc acttaaaaat gactcacgat aaccttgaac
     9481 aacatagtac agcggattta atttcaatat gcttacaaga cctgaatcaa actgattagg
     9541 gctccataat atcggtaatg tccacatgat caactgcagc acgatcgtaa ttaattgcat
     9601 gatatcaggt aaaaatggtt ggatcgatgc cgtcagccaa gtcaatcctg tcaagaaagc
     9661 acacatgcaa aataaataat aaatcagctg taaagacatt gctgaaggat atataccatt
     9721 tataagcgcg acagctatgc caatcagaat gaagaataag tgggtgtaca gattggatag
     9781 gattttcgtc gtgggcaaga tacgaatatt gaatacgact ttctttacta aataagtata
     9841 ttcccgaaaa acaaccgtag ttgatgcaaa tgcatctgag aagaagaacc agggaatcat
     9901 cccggtaact aaatatacga taaatgggta ttttgcatcg atcatttgtg ctttcagtcc
     9961 aacaccaaat accagccaat aaatcaatac agtaaatagc ggattgataa acgcccataa
    10021 gattcctaaa aacgatcctg catatcttga tttgaagtca ttgatcgaaa actgtagcaa
    10081 taattttctg ttctgataaa tgtccttcat aaataaatac aaatctttaa acatttgtgt
    10141 tcctccaagc gattttcagt gaattacagt caagtgccat tttaccatat ttttggtacc
    10201 gagctgcgtt cttatttctt tcttaatttt ctatgataca agattttctc taacgtttct
    10261 attttacaga agatgctaga caattgcaat aaaccaaaaa aggctgcgac ggttgtcaca
    10321 gcctctctcg tattttgtgc ctatcaatga tagcgttgga tcctggaaga atgagcgcta
    10381 tctggttgtt cctcataaga tgtatcatca taagtcatat cgtcatatgc ctcttccata
    10441 acatagaatt cctccgcaac ttctggttca tcatatcgat gaacggccat agccaacagg
    10501 atataaataa tcggaaccca tgggtatgaa gtcaatgtat cctccataaa tgactgcatc
    10561 atcaccgcaa aaaaggccca gataaaagtc atctgcccgt tggataaatc tgctttcaac
    10621 gttaagcgac caataaaaag gataagcagc aaagcagcaa tcccaaatcc tatcaatcca
    10681 tagatattca aaatagaatt ataactattg tggtttccta aaaattggtt tcctcgttgg
    10741 aagataaatg ttttcattcc tagtaaaatt tgttcagatt tttctcctag cgtctgatag
    10801 aatttatgcc aaatatcttc tcgaccggta aacaaattga ctttttcaga gacagccgcc
    10861 agataagaaa tcggcaaagc taaaagaatc gtaaccgtgt aagcaataaa tggcgcacga
    10921 atgacacgca tcaacgattt aggtaaaaga tccaatatcg caaaaacaat caacgcgact
    10981 aatgcacctc tagattggca gacccaaatc ccgagaaaag ctgcaacaac tgctactgca
    11041 ccgatgattc gtacatacca tcgttcaaaa gaactggcaa atccactgat caataaagca
    11101 agagtcatca aagaacttcc gattatattc gtattgatcc aaatatcaga aagcgaattg
    11161 cctggagccc agatgctttg tccttctggt acttggatct ctgtaaatac acgatataac
    11221 gtgattccga ggaagaccga aaagacaatc agataaatac cgcgatccca ttttgtccac
    11281 tcgatccatt taattactaa acaaaataaa ataatcaaag gaaacagtaa gtctccaaat
    11341 aaagactcat ttcttaattc ccgaaagtaa gaaaagaaaa tgaaaagggc aagcgcaaca
    11401 cctagataaa gaaaatcgac gccttttgct ttataaacat ttaaaatgag caaaatagcc
    11461 acactaaaag cgataagtaa tgaactattg ttatatataa acaaagttgc tggaaaacct
    11521 aaccctgctg tccaaacaaa aaagactagc gtcagtaaca aaagcaagaa accgacactc
    11581 attttgttga caaatttttg tacgattgct tgaattatct ccactctcct ttctactaac
    11641 atttcaggat tattctcagc tagcctatta ctgtacgact agctgatttt cctgagtttg
    11701 tgtttgtggt acatctggat aatccggata ttctacttta aaaatcatat aaccgtcttt
    11761 atccggtcca tatagctgcg tgaactgcgc ttcatataat tggttgtaat cagttaatgg
    11821 tcgtttactg atcacatagt cgatccctaa tttttctgcg tcttcaaaat tcaacgtgac
    11881 cgaaaacata tctggacgat ctaaaatgaa ctttgtatct tccttagcga taaaagctct
    11941 tgtatgagca taccgattat aatacttctc gtattcttct ttcggatcga ttttcttcca
    12001 tgcagctata tctggataaa agcgtactgt attgaaggat ttcactccca atgttggggt
    12061 gaagttatat aattcatctt cagacaacca aactttacca ggatcttgtt gatcgatttt
    12121 ttggatttct tttgccagtg ttttttcaaa aatcgctcct gtaccttcta cgactggatt
    12181 gacgaaaaag ccagatacga taactaaggc ggcaaaagcg aggtaaaaca agcgtctcca
    12241 gccaaataat gcaaaagcta aaatcactgt tcccaacaca gcaactgcga tggtttcaaa
    12301 atccgaaaaa taattatcca ttttcgaatg cgtaatcgca tagaaataag accatgcatt
    12361 tactgccaac aaaatgaatt tataccaaat aggaataaca tcttctctcc aaatataagc
    12421 gataaaccag atcgataata aacaagccgc aaaactaaaa gtaaggatgg ctcttaatgg
    12481 aggaacataa gacagcagag tgatttctgc tagccatttt ggcaacccaa cggtaatcca
    12541 aaagatagaa aacagacaaa aaatcatcaa aatacgtccc agtaactttt gttcgctatc
    12601 ttttttgcca aataacacaa atggacttgc taggaataca gttggaaaaa agtgatagaa
    12661 cagggccact tcagagttat tcgaatagtt gatatcttca aaaggaattt tccaatttgt
    12721 aaggaaagaa aaaaattcgc caatcgtcca ctttcctcca gtacttaccc gttttcctgg
    12781 atataaggta ttcaaagaag ctaacaatgc gtcttttgac gttagccaga aatgaaccat
    12841 gactccgcca ataaataaaa ctgcccctcc aatcaacact acatccaaca gatcccatga
    12901 aactttcttg ctatagtaaa taagcaggcc aacaaaataa aaaatcagca agtacgccag
    12961 catcacttga tgggctgggt aaatcactaa aataaaccca ataccgaaaa taaccaccaa
    13021 aagcatcatc aacgttcgta gccatttttt atcatgattg tagaaataat gataaaaagc
    13081 agtcatcagt cccaaagtaa agaaaatcaa atcgcccaca tgttgcatga accaccactg
    13141 gactgctgga gaaaatgtca gcgcaaatgc accgattagc gatagagctt tttttctctt
    13201 cgttaatatc atcagtactt cgaatccaag caagaccatg acgactaatt tcatcgccca
    13261 ataaaaggaa agtcctcgat ctcttccgag gaaaaagaat ccccagttga atggttttcc
    13321 gataaccgta atatccttta caggagaatt atacgcaatg atcatgtttt gtccgtttaa
    13381 tgaataatcc tcgttcacca acggaaagtc ctcttcagct tgtgagaggt aaaaaggtgt
    13441 ctgcaccagc cattcgtctg accgcacttc ccgattctgt ccaaaaatca catcagtttt
    13501 tttcccatcg tctctttgcg acacatattt atcccaaaca ccaagtgaac ttccgtttaa
    13561 attaaaagta acgatgacca cgatgactaa aacgccaata agatagcgga gttttagaaa
    13621 caggtcaagc accaaattcg aatctgcctg gttcgctttt ggcttttctt ttcgtcgtgc
    13681 acttcttgtt tgtttttcca tatcatgctc caaattctat ttgataaaag gaaattactt
    13741 attcattttt ttggcagctt ccagcatttg cgcaagtgct tcttgccaag ttggtatgac
    13801 aaatcctaac tcttctgtct ttttcaggct catgactgaa tactgcggac gtgttgcttt
    13861 ttgcggaaat tcttctgacg taacaggtgc aacttctaca tctgtatctt tcaatatttc
    13921 ttttgcgaat tcataccaag aacagctatt ttcgttcgac aaatgataaa taccaaatgg
    13981 tgctttttct tcgatgacat ataccataaa ttctgccaaa gtacgtgtcc aagttggtcg
    14041 cccaaactgg tcatttacta cagtcagttt gtcgcgggtt tccgctaggc gttgcatcgt
    14101 gaagacgaaa ttgtgtccat agatcccgaa aacccaagaa gttcgtatga tataatagtc
    14161 ttctaaaatc tcttctactg ctttttcgcc taataatttc gtacgaccat attcatttag
    14221 tggattcgtt tggtcgtcta cttcgtattc tacatttttg ttttttccat cgaaaacata
    14281 gtctgtactg atatagacga attgagcatt cacagctttt gctgcaagtg ctacatttct
    14341 tgtaccatca acattgatct tctcatctaa ttcttttgct tcttcttctg ctttatctac
    14401 tgctgtgtaa gctgcacaat ggaaaataac atcaggtttt aattctgtga tgtaagccat
    14461 tgttttctcg gcatctgtga tatccaagtc ttttgcatct gttgaaacat attgcagatt
    14521 tttttcatct aataaatggc gcaattctgt acctagttgc ccatttcctc cagttaataa
    14581 aaacatatga ttatccgctc cttgattttt attttaaaaa agagacaggc gagcgcctgc
    14641 ctcacatgtg tttttccatt aatacagtga cattttctct gtattattgt ccatttttgg
    14701 catatgctgc ttcaacggcc tctttttctt ttttccacca gtcttcatgt tcttcgtacc
    14761 atttgatcgt gtcagccaac ccatcacgga agttcgtaaa ctcaggcttc catcctaact
    14821 cttcccgtaa ttttgtcgaa tcgattgcat aacgcaagtc gtgtcctgca cggtcgttta
    14881 catggtcgta cgcatctttt ggttgaccca tcatttctaa gatcaattcc atcacggtct
    14941 tattatcttc ttcaccgtct gcaccgatca gataggtttc gccaattctg cctttcgtca
    15001 aaatcagcca cactgctgaa gaatgatcat tcgtatggat ccagtcacga acattctttc
    15061 cttcgccata taattttgga cgaataccgc ttaagatatt cgttacttgg cgaggaataa
    15121 atttttcaat atgctgataa ggaccatagt tgtttgaaca gtttgaaatg gttgcacgta
    15181 agccaaaaga acgcacccat gcttttacta acagatctga accagctttt gtagaagaat
    15241 atggacttga aggattatat ggtgtttccg cagtgaattt ttctccttcg ccttccccat
    15301 gtcctggcag gtcttcacgt aatggaagat cgccgtaaac ctcgtcagta gatacgtgat
    15361 gatagcgtac atcatacttg cggcaagctt ctaaaagtgt atacgtacca atgatattcg
    15421 tttgtacaaa tgggaaagga tcttttaatg aattatcgtt atgggattct gccgcgtaat
    15481 gaacaactgc gtccgcttct tttactagac ggtccactaa ttctgcatct gcaacatctc
    15541 ctacgaccaa ttctacgcga tcgctaggaa gtccagccaa gttctctttg tttcctgcat
    15601 aagtcagttt atccaaaaca gtgacatgca cttctggatg attgtttacc acgtaatgaa
    15661 cgaaattaga gccgatgaat ccggcacctc cggtaacaat gatgtttttc ataagaaaat
    15721 ttctccttaa ttcttagatt tctccgtaaa caaatggatt ttctgcttca aattctttta
    15781 atgtcggatg acggtgatct ttttccgaca taatcgctct tgatggatca attggccaat
    15841 caatcgctaa atctggatca tcaaatgcga tgcccccatc tgcttctgca ttatagtaat
    15901 tatcacactt ataaaggaag ttcacatttg gtgtcaaagt aacgaatcca tgggcatatc
    15961 ctttaggtac aagcaactgt cgatgattat gttcagataa gataaatccg tcccattgac
    16021 cataagtcgg gctacctttg cggatatcca ctactacatc taaaactgct ccagtgacta
    16081 cccgaatcaa ttttgtctgt gcagcttctc ctttttggaa atgaagcccc cgaaggacac
    16141 cagcttcagc tgatagtgaa tggttgtctt gaacaaagtc atatttcaat ccatgttctg
    16201 caaattttgc ttttgaataa ctttctgtga agaaaccgcg atgatcgcca aagacgtcca
    16261 tttctacgat tttgacatct gttaattttg tatctgttac tttcatctgt aaggactcct
    16321 tattcagcgg ccaaattcaa taaatattga ccgtattgat tttttttcaa tggctgtgca
    16381 agggccagca attgttcctt atcgatgtag cccatacggt aagcaatttc ttccaaacaa
    16441 gccactttta agttctgacg tttttcaatt gtttcgataa aggtagatgc ttcaagcaat
    16501 gattcatgtg ttcctgtatc aagccaagca aatccgcgtc ccataagttc tacggatagt
    16561 tttcctttat ccaaatatgc tttattgaca tccgtaatct ctagttctcc acgttctgaa
    16621 ggtttgatac ctttagcgat agaaattacc tcattgtcat aaaaataaag gccagttaca
    16681 gcgtaatttg attttggctg agcaggcttt tcttcaatcg acaaagcttg catattctca
    16741 tcaaattcta ctacaccaaa gcgttctgga tcattgacat gatagccaaa tactgtcgcg
    16801 cctgtatctt ttgaagctgc tcgttgcaac attttagaca gaccgccacc atagtaaata
    16861 ttgtccccta ataccaagca gacactatca tctccaataa attcttctcc aatgataaac
    16921 gcttgtgcca atccatctgg actttcttgt actgcatatt cgatctgcag tcctaaatca
    16981 tttccgtttc caaataattc cttgaaacgt ggtgtatctt gtggcgttga aataatcaga
    17041 atttctttga ttcccgccaa catcagagta gacattggat aataaatcat tggtttgtca
    17101 taaatcggca tcaattgttt cgatgttgcc tttgttaaag ggtacagacg tgttccgctg
    17161 cccccagcaa gaatgattcc cttcatggat atctgctctc ctttttgatt tcaaatttca
    17221 ctttgttatt atctcatcaa ttgttatatt tgtcatggaa atattaagga tttaaacatc
    17281 ttttatcaac gtttaaacca ttttcgtttg atcaacgccg taatcagatg aacacgccaa
    17341 gaaatttttt gccgtaattt agaatgtgtt tcaatctcac tcgcgtttaa atcatgtctt
    17401 cggtacagag ttaacggttc tcttaggaac ccagtcttct ttcctcccag caaaccaatc
    17461 cacatatcat gcatcggcac ttctgacggg atcggcaaaa tatcgggttt taaagaagac
    17521 cggaaagcca taccagcacc aatatagtga cttcttatca cattattaag ccatccttct
    17581 ttggaatttc gaaacgaaaa atacgaatca tggatctctt gcagctcgct gtccacaatg
    17641 attaaatcag aaactactac tagctgatcc ggattctgct caaaatagtc agtgatcttt
    17701 ttcacttttg tctccaacca aacatcatct tggtctgcca aaaagattag atctcctttc
    17761 gtctgctgga tcgcataacc aaaattagcg atcaatcctt gacgaggccc cgaaaaaagc
    17821 tggattcgct tatctttttc tgcgtattca gttaatatat cccatgtatc atctactgac
    17881 ccatcatcag aaatgactac ttcatcttct gctgaaagct gagataaaat actatctaac
    17941 tgctctctta aatatctgcc tccattaaag gtagctatac aaaccgaacg cacgaattca
    18001 caccttccaa ttcttaacat atgagcgcaa agtcatccgc cagatatgcc ggtttttgac
    18061 ggtcaagaac tgtttagctg ttcttaacaa taattgtcgt cggtgactgg ataaatgcct
    18121 ttgatcatat tcatgataaa atcgattttc tgcttgtaaa atagaacggt atcgattact
    18181 tgaaactttt tcatagtcta aaaccgaaag atcatgttcc attcgtacag gaaggatctc
    18241 cacagttttt tccaactgat ccactttcca aaacagccaa tgatccaaaa aatctaaaga
    18301 aaaagcagga ttaaacccgc caatcttttt tagaaatttg acagaccagg caacacctga
    18361 attgattgcc atgattcgtt cagctgttgg gccttcaact tctactgctt gagctgtacg
    18421 attgatataa cgatctgaat ataggggaga aacttgtcgg ctcccagaaa aaatcatcgg
    18481 aactgcagca acacattcat ctgtaaaggt tactttacgc aatagttcaa aataatcgtc
    18541 gactagcctc gtatcctgat ccagagtgac gagacaacgt gttccttcat ctgcttgaga
    18601 caacgcaaaa ttataagcca ccgctaaacc aggattttgc ggatcatgat gataaacgat
    18661 attttctttt tcaaacagct cattttgctg cctttgagga ctattatcgt aaataataag
    18721 ctgaatttgc ttttcgctaa gtgctttgct taatggtcca taagctggag attgactgaa
    18781 tagttgctga taaagcacaa cgactactaa tatttctggt ttcatcagaa aaaacgccac
    18841 ccccacttat tgaaaaattt catcatagaa gtcaaaaaca cgcgaaacag ttttgcattt
    18901 cgatgcgcac ccttcccgta aaaatggata actgcataaa agggtgtgta caataacttg
    18961 taacctgctt tttcaaaact caaacacaag tcgttgtctt caaagtacat gaagaaacgc
    19021 tcatcaaacc cctttatctc tttaaattta tcaatatcag ctaccataaa actacctgag
    19081 atcatccgta cataactatt agcatcagta ggcaatcctc ggcattcaaa cacagccagt
    19141 cgtttgtcaa actttttttt gacccatttg aaaggcacaa agcgcaaaac gtaatcaaaa
    19201 acagccagct gctcacggat gaggtgctga gttgttccat cttcattaag aatcttaggt
    19261 gctaacatcg cacattccgg atgttctttc aataacaaga ccaatgctgt aactgtctct
    19321 tcatccacta aaatatctgg gttacaaata atgccatagc gagaatcgct agctatcaga
    19381 ttgtgattat gtccataacc aaatccggaa ttttcatcat gaaaatagat atcgatgtag
    19441 ggatatttcc tatactctgt tagtcttttt ttgtactcgg gaagtgaatt gttgtcaaaa
    19501 attcggattt ttactgctgt ttcatctttt acagttgaaa taaaattatc tagtgtccga
    19561 aaaattttat cactgtttgc cgtcacaata ctaataagga tcggctggtt taatttgtta
    19621 ttcatctata tgacggttcc atttcttcaa atatttctgt tttacttgcg tacggtactc
    19681 tttattccct aaaaatcgca atgtttttaa aataaactga tgtttctcat tgatcagacc
    19741 aatcaattca aataataaaa ttgcagcaaa caacatggca ataatcaata tgatcgttcc
    19801 gattcttcca gtataattca tgaccatagc tgtgaaagaa aacatcaaag ccatcgcata
    19861 aatcgtcagg acagcacctt tatgagtaaa gcctaaactg attagtcggt gatgaagatg
    19921 ttttttatct gctgtagaaa aagaaacacg gtttgctttt ctcctaatga tagcaaaaaa
    19981 cgtgtctgtt accggcactc ccatgacgat cagcaaagaa atcgatgaaa caaaagtcac
    20041 gttcttcagc ccttgtagtg aaaggacggc catcataaac cctaggaata gagctcctgt
    20101 atcgcctaaa taaatctttg ccggataaaa attgtaagga aaaaaaccga taatacttgc
    20161 tacaagacag aaaataacaa ctggaatata cactgtagaa gcatggagga aaaaatatcc
    20221 aatgattcct atcgtaatta aaccgatcaa tgaaatccct gccgccagcc catccaaacc
    20281 gtcaatcaga ttgaccgcat tcgtgatacc gaagatccaa aaaatcgtta cgggaaaact
    20341 aagccagcct agttggatat atccaataaa aggaagcgtg aaggaatcaa tacgtatatc
    20401 tgctacaaaa taaataataa gagaagcaat caagatccct atactttttt gtttaggtgt
    20461 cagctcatat ttatcatcga gcactcctgt gactatgatt actaaagaag caatcaagat
    20521 cggtacaata tacgattgag ggataatttt acggaataag aataaagtcg atataacaaa
    20581 tgaaataaag ataggcagtc cgcccgctga aggcattgga actttattta ttcttctttc
    20641 atttggtttg tcgaccatac ctgtttgtac tgaaatgatt ttgaatacag gtgttaatat
    20701 cagtgataat aaaattgtca gaaaaaatct tataaccata ctaaaagaaa caagcatatg
    20761 ggcacctact ttaattaaga gtcataatta ttataaatta tatatgtctt tttgactatc
    20821 tttttatatc ttctcaaatg tccgcaaagc cttattttat cggctctacg ggcattttgc
    20881 ttttgtggta aacctcgcat atctaggtct atcttcttat attttccctg tcaatcgtgg
    20941 ttaaaatcgt ggtaaatact tctatgcgat tagacgctga acctctgatt ttgcggtatc
    21001 tatggacgca tgagcgtacc agttcattgt gatactgatg tttgaatgcc ccatgatata
    21061 ctgtaaatct ttcgggttca tgttcttgct tgccagcctt gtgcagaatg tatggcgtag
    21121 cgtatgcggt gtaatatgtg gcaagggatt gtccttgtgg tgcttgttgt atttctttac
    21181 catacggata aataaggcgt tataatcaat ggcaactttt ggattacctt tcggattgat
    21241 aaacacaaaa ccgctgtgtc cgtctacctc tatcgacttt atctttggtc ggctgttgat
    21301 aacccgttga aatgcctgta tcgtttcttt gcttaatggc acttgcctta tcccgctttt
    21361 cgtcttaggc gtttcaatat aatagccctg ttccttactc tttagtaact ggtggtcgat
    21421 aatcacaact tcattcttga aatcaatgtc ggctactgtc agtccgcaca gttccgagat
    21481 acgaagtcct gtctttaaca gtatcagcac atcatcataa tgcttgtgat acacgttgtc
    21541 cgtcttaata aatgacaata aggcttgctc ctgttcctct gtcaatgcga ctttctcttt
    21601 ggtatcattt tctatgactt cacttagctt gaaatcaaaa gggtttttcc tcacataatc
    21661 atcttgtatg gctatgtaga atgacgcttt taacgagcgt ttatggttgt taatggtatt
    21721 gtaggaaaag cctttgtctt tcatgcgtaa cgcccattct ttagcgtcag agggttttat
    21781 cgtatcaatg ctcctagcac ctaacttgtc ctcttttaat aaccgcatga gttgttcccg
    21841 ttgtttctgt gtgcttttct tcacatttgt cctctgtgcg ttctgtttgg catagagttg
    21901 gcaaagcgtc attttcttgc ctgtgctgtc gataccgtcc tcaatatccc gtcttatctg
    21961 ctgttccagt tctcgaagtg agggtttttc ccgttttccc tttggtgtcg ggtctgtggg
    22021 tgtcaatctc caagcataca catattttgt gtttcccaat gcgtctacat atttatataa
    22081 gtattttccg tctgttcgtt ggctctctcc agtatgcagg atacgacctt tgttatcccg
    22141 tcttttttct ttcatggtgt ctgctccttt ccttgttgga aagagccttg atatgacttg
    22201 tgttcatcat aacacataca aggctcattt gcattagatt gcgtccaatt tatcaataac
    22261 cttttcaaac tgtcggcgtt taatctgtat gcggttgcca ttcataatga gccagccagc
    22321 gtccttgttt tcctctgcca atcgtcttaa cttgttttct ccaatgcgga aatactttga
    22381 cgcttcttca atggtaaggg tgtatttttc ccatacggga atatcctgtg tattactcat
    22441 aaaacacccc ctcactttcg attacgtctg atatgtccgt acacatctgt tccacaagca
    22501 tttcctgttt ttcagaaagg tgggtacatt catcaatcat tttcatgtag tttgaacctg
    22561 tcagcctgtc gatttcctct atcatcacga ttgacggcat gacctgtctt tccagccatt
    22621 gcagagtgcg ttccatattg acaggtacag ggttcatttc aaaaggtaaa ctgtcatggg
    22681 aaatgaatat ctcccacaat ggatagtcag gaaattccac aaaatcggta atgagataaa
    22741 acaccagccc atgagggtta tacgtcaata acagttcttc aactgcctgt accgcctttt
    22801 tgtcacgcag acgaatttca aaacggttga taatatcggt atgtttcttt ttcgttgcct
    22861 gttccttttg tttttcatac aggcagaaat attttgtgct tgcctttgag ccgatataaa
    22921 gggtacttgc taaattacgg ttttttccgc ttaattttcc accttgtaca ttttcatagt
    22981 ttttacagcg acattccgca ccaccatttc tgtatttttc agatagcaca ggaatatcca
    23041 gcagaccgca catatcattg attgccaagt caaaacgtcg gataacaccg ccacagtcaa
    23101 gacagcggtt taagaatgaa taccagtccc tgttctgtgc ctgtaagaca tattccatgt
    23161 tgcgtgagcc tgcccctttt aattctaaaa atacgcccat gtgttcatca tcagaagcca
    23221 tgaccttgat ttccccgtaa gcataatgct ccttatagcc ataataccca tagtcataat
    23281 ggataaaata atctgctttc atgccaagca cctttcggat aacttccaat gcgtcagtcg
    23341 ttggaaaacg gacagacaga taatcaacga gcagataaaa aggcttatcg cagatatagt
    23401 aattcagaca acgcaggata atgttctgtg tttccttgct ggcgttagct tttccgttct
    23461 caaagcggtt gatactatat cttgatacat gggtaagact tgcaagttct ccttgtgaaa
    23521 gaccgctggc aagacgtttg ttcttcattt caagtgcaaa ttctaaattg ttcattgtat
    23581 tcctccaatc ccacaatagc cttgaaatac ctgtaaacca cctttcaaaa agtgtgatat
    23641 gtactatctt cttaaaacct ctcattacta ctcacaggtt tgggggtgta tggtttttga
    23701 cttgtctttt gtgaaatagt gcccccctgt tagataacgg gggcacagac aaggctcgca
    23761 ggctcgccag ccgacaaagc atttcttccc cttgacacaa ggtgtcatgg cggtaagcct
    23821 gagaggcttc cgccaccgtg tcagaaatac tttgtcagca agttctgttt tgccttttct
    23881 aacaagttcc atttcaaccg atattcatat ctacggattt aatcagatag gtatttcttt
    23941 ttgaatgttt ttatatttca ttctattgtg cttattcgat tacaagaaca tagtaattct
    24001 tatatgctta tttgtcaata tctttttttg cctttacaaa tcatattaag catgatagac
    24061 ttataatagc gaagatataa cgagatatgg agggttatgc aatgaaaata agccatttag
    24121 gaaataatat acagaccata agaaaattta ggggaatgaa acaacaggaa cttgcggaca
    24181 aaatcggtat caatatgcag agcctttcca agattgaaag agggttgaac tatcctgcct
    24241 atgaaacgct ggaaaagata atggaagtgc tggacgttac gcccaatgaa ttgttgtcgg
    24301 gagaatggaa gtatgtcaat caatctgaaa aggaagtctg tcagttttta agagccgaag
    24361 aacgtctaaa tgcagaactg aaacatggac actatgataa cttctttgac agcgaggaag
    24421 aatggctgga atatgagtta gaaaagttac gggaatatat tacagactat atcaacggga
    24481 aaagcattga agcgtccgac ctttacccaa tcaaagaatt tatccagcat ttgaagtttc
    24541 aaaaattatt agaccgctat gatgatttat acagtatgga tatgtttggg gaaagcatag
    24601 agggacataa gtataagaca ccttatcaag tcgtgaaaat gataaacccg aactcaaaag
    24661 aagatatgga actgttacga gaagtattaa ggaataacaa ttttgatgat gaggacgagt
    24721 aacttttttc ctcttgcgag aagatacagc aaacaaaaag cccgatcaag agtgcaaagc
    24781 accaccaatg gtggcaaagc tcttgacagg gctttttctt ttctgttgtg ggcaatcaag
    24841 aggaagaaag ggataaaatc tgatttgtgg taaatcaggc aaaggcaacg caggtatcac
    24901 agtatgaatt gacaccgcat aaggaaagca actggaagat tgtagaataa atgaaaaagg
    24961 cttgcattct gcatttttat aaaattgcaa gtctttatat tgacaagcat aaaatactaa
    25021 gatatacttt aatcagacca acggtttgta ttggaggtaa gttatggcac gaaataaata
    25081 tccagaagaa acagtaaaac tcattttaga tgtttctaca aaaatgtttt gtgaaaaagg
    25141 atatgataac acatcattgc aagatattat caatgaaaca aaactttcta aaggtgctat
    25201 ttatcatcat ttcgattcaa aggaagatat tttaaaagca atttttcaaa gaattggaaa
    25261 tgaaaatgca gtaatatttg ctaaaattag agataacgat agtttaaacg gtttagaaaa
    25321 gttaagagaa atatttaaga cagcggtatt caattctaac caaagcgttt tattgacagt
    25381 ttctcctcaa ctattaaaca atcctcgttt tcttgctatg caaatagaac aaatgtatga
    25441 aattgtagta cctgaatttg ttgaacccgt tttagaacaa ggtatcaagg ataagtcttt
    25501 ggagattgaa aattcacatg aaattgcaga agttatcatg gtattaagta atgtatggct
    25561 caacccatta gttgaaatga cggataaaga gggtatggaa aaacgttgca atacattcaa
    25621 tgccctttta aatggaatag gcatagacac attattagac gaagaaacaa tttctgcatt
    25681 tatacagttt tgtaaataac tgtatttgtt atccctatac gatagactgc tctataaaag
    25741 agcagtttat ttaaaccatt atataaaccg acggtctgtt tgattaaata aaaaggagag
    25801 attttatgca agcagaaaaa aaacttttta atcgtgattt tatacttgtt gttataggac
    25861 agataatttc tttatttgga aacgctatcc tacgatttgc attaccgtac tatttgttaa
    25921 aagaaactgg ttcatcagct ttgtttggta ttgctacagc ctgctctttt ttacctatgg
    25981 ttattttgtc gctggtaggt ggtattattg ctgacaggac aaataaaaga aacattatgg
    26041 tcatactaga ttttgtaaca gcaatcataa ttttagtatt ttatttgtca ttgggaaaaa
    26101 ttcctatcat acctttgttt attgttgctt tgatgttact ttatggtatt tctggtgcat
    26161 atcaaccagc ggtacaagca agtattcctc tattggtatc atcagataaa cttaccgtcg
    26221 gtaatgctat aataaatcaa gtaaatactt tagcaaactt tatcggacca gctataagcg
    26281 gagtagtttt tcaattttat ggtatcaatc ccattttaat tgttagtatt atatgtttta
    26341 ctttttcagc cattatggaa atttttatcc atattccaca ccaaaaaaga cctcgtgaag
    26401 taggtgtaat tgcagttgct aaaaacgatt taaaagatag ttataccttt ttgaaaaagg
    26461 agaaacccat ttttatatcc gtaatctttt tggtttgcat tttcaatttg gttttaagtg
    26521 ctgttttaat tgttggtctg cctatcttaa tttctgaagt tttaaaaatg ccagatacac
    26581 tatatggttt ttcacaatca gcattatcat taggtggttt atgtggtggt attttaacag
    26641 ctcttgttgc ttcaaagtta aaactaaaaa acctttatac cttgttactt atttgttctg
    26701 tttctgtttt acttatggga atttcgctat ttttagaatt gcctgcaata gtttcctact
    26761 gggtagttac agtgatgtgc tttgtggcta tgggtgtatc tactatgttt gttgttcaaa
    26821 tttacactct tgttcagaca caaacaccac cgcaattagt aggaaagata atggctacgc
    26881 tggtagcagt tgctatgtgc ggtcaaccta ttggacaaat ggtttacggt attttattcg
    26941 atgtatttat acaagaaaca tgggttgtta tgtttgtagc ttctattgct tcaatcatga
    27001 tttcaatata ttcaaaaaga atttttcaaa agttagagat ataatacaag gggttaaata
    27061 gtatgttagg ctaaaaacaa tatataaaag aaaatttgaa atctgctaaa aggatagtgt
    27121 tataaaagaa agagtaatat tgcagtattt aaggctcgca caaatatatg ttttgtgtgg
    27181 gcttttttct taccattttt ctgtatttct aaagggtata aacggtattc cgtttatata
    27241 tttcagcatt agcagaaagg gggtgagatt atgaggacat cttctttcga gcatatcgtc
    27301 agattacagt tcaattcttt aatgctgatt gtcattaaga acaaagtgaa aagtcgaaac
    27361 agacagcttg caagacattc taaacgtgaa gtattgtttt gtgaaatagc cgaaacgaaa
    27421 caagttgaac gtggaacgat tgatacctgt ttttatgatt gtgtatcatt taaagtcttg
    27481 cactttacaa tttatgtatc tgatgaaaca cttggtacag ttttacatag actgtcagaa
    27541 aaacagcgta gtgcgatttt attacgttat tttcaaggaa tgaacgacag agaaatttca
    27601 gaactgtacc atgtatcacg ttctgctatt tgcagtagaa gaactagagg actaaggaag
    27661 ttaaaaatgc ttttgagcga aaggaagtaa agaaatgagt aaagattatg gctatccctc
    27721 ttttgaattg atttgcaaag cgtcaagcgg agatgaaatg gcagttaaag agattttgaa
    27781 gttttatgac ggttatattt ctaaattatg cttgcgtcct ttttaccact ctgaaagtgg
    27841 taaaatcatt atgcaggttg atgaagaact aaaaggagaa attcatacag acatgatgaa
    27901 agcaatattg aaatttgaaa tcggagtgaa gtaatcaact gtataaaaaa atcgggagat
    27961 acacagtttc gtgctatctc ccttagtagt tttacggtca tatcaaggct cattcaatcg
    28021 tggtaaattc gtggtaagat gagtgttttt ctatacttca aaatgcttga atgtatagtg
    28081 tttatgcgga taattgaaaa ttagatataa aatttcaaat aaattactga tataaattta
    28141 caataagaac tcttgtttct actttttttt atgagaaaat ttagttttca atttattgaa
    28201 ccattttcgg cttggatctc gacgtttttt gatttgatga cccgtcacgt attcttgaac
    28261 aactgcattg atgattccgc ccaaaatgat gatcgtagca gcaaaattaa gccaaagcat
    28321 gagtacaata aaacttccta tgatctgata accgctgatc ctcgttgcaa aatacctagc
    28381 atagatacta aacacttgtg ataacagcat ccagccgaca gtagcgaaga tcgtgcccgg
    28441 aatgatcgtc ttcaacttca actgagcatt agggacaatt aaataaatca agaacatgat
    28501 gacaaataaa gctaccaatg tgattggcca cttcaaagtt tggaactgat cgataaagtt
    28561 cacgggtatt tgtacgattg gctgaatagc atccaaaatc agctgtccca acccaagaac
    28621 gatagttacc ccactaatag caatcatgaa caagacaatg acaattagtg agaagaaacg
    28681 gacgatcaaa aaattttttc tattttctac gccatacgct ttattcatcg caatttgcaa
    28741 agcattgata ctttgacttg cagaccataa agccgctaat gcagaaacgg ataacaagcc
    28801 tcctgaactt tgtgtcaaaa ggtctttgat tgcaggaccg ataaactggt aaacggtctc
    28861 aggaatcact tctcgtaaat aggtcagtac agtctcttga tcgatgtgga gaaacggtaa
    28921 aatattacca aaagcaataa tcaatggaaa taaagacaag agtaaataat aagcaacgac
    28981 gacagaggtc gtactcattt ctgaatcagt gatgcgcttt tgagtcgtct caataaagcg
    29041 catcagattt tcattttgtt ttattttgtt ccaaactttc atgtacctct cccgtaccta
    29101 ttgactggat agatcaataa gtgccttctt ctccttgtga tgtcaaaatt cttggaccat
    29161 ctttagtaat agccaatgta tgttcatatt ggcaagaaag tccgccgtct cttgtatacg
    29221 cagtccagcc gttaggatcc attttcattt tccaagttcc ggtattaacc ataggttcga
    29281 tcgtgataac catgccttct ttcaaccgca acccttttcc agcttctccg taatgaggaa
    29341 ctgctggttc ttcatgaatc gtaggaccaa cgccatgacc aataaaatca cgaacgatcg
    29401 ctagattttc gccttctaca taagtttgga tagcatgacc gatgtcacca atccgattcc
    29461 ctacttgtgc ttgctcaata ccgatgtaca aagctttctt cgttacttcc atcaatcggt
    29521 cgatctcagg tgtagattct ccaactacat aggcccaaca agaatcagaa atgccgcctt
    29581 tcaaatcgat acacatatcg acttttacca aatcaccgtt tttcaaaggc tttttacgag
    29641 ggaatccatg acaaatttca tcatttatac tgatacaagt cgcatattca tacccttcaa
    29701 agccaatttg cgcagcaatt ccgccatgac tctcgatata gtttctgaca aatacctcaa
    29761 tatcccaact tgtgattcct ggttttataa aatcacgcag atttttatgt acatctgcta
    29821 ataaagctcc agattctgcc atcatttcaa tttcacgtgc tgattttaaa gtaatcatat
    29881 tttcgtctcc tttttacaaa tccctctcat tttaacatag cggcatacat ttagccaaat
    29941 gacattcccc aggtgtctct aggttttatt ttcttttcaa tgatgttttg gtataattca
    30001 taattgttaa ataattataa acgaggaggc acatcatggc tttagcaaaa atcgtatatg
    30061 ctagcatgac aggaaacact gaagaaatcg ccgatattgt tgcagaagca tttgaaaatc
    30121 tggatatcga agtcgaaatc aacgaatgta cccaagtaga tgcagatgaa ttcgaggagg
    30181 cagatatttg cgtagtagct acttatacat acgatgatgg cgatctgcct gatgagatcg
    30241 tagactttta tgaagacttg caagaattgg atctattggg aaaaatttac ggtgtctgcg
    30301 gatctggtga tactttttac gatgaattct gcaaatcggt ggatgatttc gatgctgctt
    30361 ttgcaaaaac aggcgcatca aaaggagccg aaaatgttaa agtggatctg aatgctgaag
    30421 aagaagatat cgaaaattta gaagctttcg ctaaagaact agtcgcaaaa atttaatata
    30481 cttgcaataa aagagccatc cagatttatt caatctgtat ggctctttta ttcattgcat
    30541 tcatggaaga gttcttcggg agttaaaaat gcatacgctg taagaaaaac gaacttttcc
    30601 ttattttgac acatctcttg ttacctactg atttgtggaa cttttgagta tattcaatcg
    30661 atttcgattg gttctttgaa tttcaagata gcatctatac tgtcttggat catcttttca
    30721 atctgttcat cagttggatg gatgatgctt gaagacatca aagctgcgat cccagtagct
    30781 atgatccatg tcccattata caaagattcg atctgctctt ctgaaagatt cacataagct
    30841 ggatcgcgtt tcactgtatt cacaaagtaa gtataagaga aattctgcat ctcttttcct
    30901 ccaccatatt cttctaagta cagtgaacga tacaacttct tctctcttct tgcaaaatgg
    30961 atataattaa aagcaagatc gataatcgta ttccctgtat gctcaacagt gaacacttca
    31021 tattgtaaat gatcatagat ctgctcaaaa agagcttgtc tcaagtcatc catattttta
    31081 aactcaagat aaatcggttg cgtggaacaa ttcattttgt tcgcgatgtt tctcgcagta
    31141 aatcgtgtaa atccatcggt ggcagctact tcataagcag ctgccaaaat ctgttctcta
    31201 gtaatcgtct tttttcttgc catatttttt aatctcctta atttttttat aacgcttcca
    31261 ccattgatta atttcattgt aacatgtttt tttgatatgc aaaattagtt tttgattttc
    31321 ttcacaagct gaaatagcaa gtagtaacat atatcatgaa acttaacctt aaaaaaataa
    31381 cctccttttt ttatttaaag cctcattgtt tgaatcataa t
//
LOCUS       DAYYAO010000027        31070 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000027 DAYYAO010000000
VERSION     DAYYAO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.028"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACHARI_001143"
     CDS             complement(287..649)
                     /locus_tag="ACHARI_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442201.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACHARI_001144"
     CDS             complement(723..1103)
                     /locus_tag="ACHARI_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8442202.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACHARI_001145"
     CDS             1185..2360
                     /locus_tag="ACHARI_001145"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8442203.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACHARI_001146"
     CDS             complement(2437..3021)
                     /locus_tag="ACHARI_001146"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8442204.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACHARI_001147"
     CDS             3222..4220
                     /locus_tag="ACHARI_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8442205.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACHARI_001148"
     CDS             4332..4841
                     /locus_tag="ACHARI_001148"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8442206.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ACHARI_001149"
     CDS             complement(4871..5530)
                     /locus_tag="ACHARI_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8442207.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ACHARI_001150"
     CDS             complement(5532..6296)
                     /locus_tag="ACHARI_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8442208.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ACHARI_001151"
     CDS             complement(6304..6981)
                     /locus_tag="ACHARI_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8442209.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ACHARI_001152"
     CDS             complement(7018..8403)
                     /locus_tag="ACHARI_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8442210.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ACHARI_001153"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHARI_001153"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACHARI_001154"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACHARI_001154"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8442211.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ACHARI_001155"
     CDS             9342..10199
                     /locus_tag="ACHARI_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8442212.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ACHARI_001156"
     CDS             complement(10280..10615)
                     /locus_tag="ACHARI_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442213.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ACHARI_001157"
     CDS             complement(10682..12052)
                     /locus_tag="ACHARI_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8442214.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ACHARI_001158"
     CDS             complement(12130..12666)
                     /locus_tag="ACHARI_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8442215.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="ACHARI_001159"
     CDS             12975..14456
                     /locus_tag="ACHARI_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8442216.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="ACHARI_001160"
     CDS             complement(14559..15266)
                     /locus_tag="ACHARI_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8442217.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="ACHARI_001161"
     CDS             complement(15345..15683)
                     /locus_tag="ACHARI_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8442218.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="ACHARI_001162"
     CDS             complement(15794..16756)
                     /locus_tag="ACHARI_001162"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8442219.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="ACHARI_001163"
     CDS             complement(16771..18102)
                     /locus_tag="ACHARI_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8442220.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="ACHARI_001164"
     CDS             complement(18110..18790)
                     /locus_tag="ACHARI_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8442221.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="ACHARI_001165"
     CDS             complement(19009..19488)
                     /locus_tag="ACHARI_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8442222.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19615..21294)
                     /locus_tag="ACHARI_001166"
     CDS             complement(19615..21294)
                     /locus_tag="ACHARI_001166"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8442223.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21842..23344
                     /locus_tag="ACHARI_001167"
     CDS             21842..23344
                     /locus_tag="ACHARI_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442224.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23357..24019
                     /locus_tag="ACHARI_001168"
     CDS             23357..24019
                     /locus_tag="ACHARI_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442225.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24120..24323)
                     /locus_tag="ACHARI_001169"
     CDS             complement(24120..24323)
                     /locus_tag="ACHARI_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8442226.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="ACHARI_001170"
     CDS             complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="ACHARI_001170"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8442227.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="ACHARI_001171"
     CDS             complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="ACHARI_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8442228.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28287..28901)
                     /locus_tag="ACHARI_001172"
     CDS             complement(28287..28901)
                     /locus_tag="ACHARI_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8442229.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29507..29884)
                     /locus_tag="ACHARI_001173"
     CDS             complement(29507..29884)
                     /locus_tag="ACHARI_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442230.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30184..31065)
                     /gene="galU"
                     /locus_tag="ACHARI_001174"
     CDS             complement(30184..31065)
                     /gene="galU"
                     /locus_tag="ACHARI_001174"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8442231.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         9269 a         6456 c         5185 g        10160 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taataaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt
    19681 ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc
    19741 aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt
    19801 catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga
    19861 aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga
    19921 caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat
    19981 cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag
    20041 cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa
    20101 atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt
    20161 catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt
    20221 ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac
    20281 gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat
    20341 cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat
    20401 tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt
    20461 aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat
    20521 tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa
    20581 tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca
    20641 atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg
    20701 attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc
    20761 caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc
    20821 cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt
    20881 atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc
    20941 taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc
    21001 taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg
    21061 aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat
    21121 gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc
    21181 aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat
    21241 gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt
    21301 cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat
    21361 gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag
    21421 gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt tggatatact
    21481 tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca
    21541 gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa
    21601 caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc
    21661 atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac
    21721 tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt
    21781 taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa
    21841 tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt
    21901 attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag
    21961 cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag
    22021 tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac
    22081 tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga
    22141 aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat
    22201 ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac
    22261 tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc
    22321 agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac
    22381 agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt
    22441 cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac
    22501 gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag
    22561 cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta
    22621 cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc
    22681 tgctatttac actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa
    22741 gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt
    22801 ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc
    22861 cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt
    22921 aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga
    22981 agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc
    23041 gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca
    23101 agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg
    23161 tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat
    23221 caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt
    23281 ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa
    23341 ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca
    23401 agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt
    23461 aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc
    23521 aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta
    23581 tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc
    23641 tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca
    23701 agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt
    23761 gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga
    23821 acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga
    23881 tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac
    23941 tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt
    24001 tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct
    24061 gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt
    24121 tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct
    24181 ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg
    24241 tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta
    24301 gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct
    24361 tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag
    24421 ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt
    24481 ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga
    24541 tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac
    24601 gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca
    24661 cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat
    24721 gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat
    24781 ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat
    24841 ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt
    24901 tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa
    24961 attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt
    25021 ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac
    25081 gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg
    25141 atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag
    25201 aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc
    25261 cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt
    25321 ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga
    25381 cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac
    25441 agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct acttccactc
    25501 cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta
    25561 catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac
    25621 atttttcctc tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat
    25681 aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg
    25741 tttggattac ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa
    25801 tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg
    25861 cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg
    25921 gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg
    25981 atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg
    26041 aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa
    26101 tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg
    26161 aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta
    26221 cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga
    26281 ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa
    26341 agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc
    26401 agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat
    26461 tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg
    26521 aactttctgg ttttttacgg attttttctg catatatcat catataacca gcggctaatg
    26581 cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag
    26641 caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt
    26701 gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa
    26761 atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac
    26821 cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac
    26881 tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac
    26941 ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata
    27001 ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata
    27061 ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt
    27121 aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc
    27181 cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg
    27241 gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat
    27301 aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt
    27361 attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa
    27421 agatgactac accaaaaata atgacatacg tatggggtgt cgataattta gtgaagtcgc
    27481 gtttcggttt tcctgaagaa tttgttttct tcaaatttat ttcttccttt cttttttcag
    27541 taattttgat gaataaaaaa tggataatca aatgaattat acagtaaaaa tgacatttcc
    27601 acatgaataa tttattagaa aatcactttt ttctattttg ttttttttct cctatgtatg
    27661 attatttttt tctctttcca tttatattaa gtattttatt tctctgtttt tccttaaaat
    27721 tcttttttta tttttagatt agagaaaatc tcgtcctgtt tttttcattt tacaatcatt
    27781 gaaaaattga atttcattgc aaaaaaaata ataaacactt ataaatatcc attatctatc
    27841 cttcaatttt tgtgtatttt atttctatta gaaaaaggta tcctgttgga caattttgtt
    27901 atatcaggta aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga
    27961 gttttgggga agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat
    28021 tctttcttgt ctctttttcc actacttttt atttttctga cactacatat tcttataact
    28081 catttctcat aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta
    28141 aaatcaaaga tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct
    28201 cattaataat cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt
    28261 aaggaacttt gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt
    28321 gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag
    28381 atcgctggat ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc
    28441 aaaaccaaat cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt
    28501 cgtgacaaat caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga
    28561 gctagttcga tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct
    28621 gatttgacag aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca
    28681 cctccggctg gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat
    28741 tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta
    28801 atttgtttcg cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc
    28861 tcttccaatt ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct
    28921 atacttgaca tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt
    28981 tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac
    29041 gcccaatacc ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag
    29101 gggtagaatt ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta
    29161 tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc
    29221 aactaagggt cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta
    29281 atctcttatc caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat
    29341 ataaataaca taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag
    29401 attcttttat ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa
    29461 tagtttatcc attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc
    29521 tcgttcttct actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt
    29581 ttctgcctat ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag
    29641 caaaaaataa agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg
    29701 tagcaacccc aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga
    29761 ccatgcgaaa catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc
    29821 ttccttccat cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc
    29881 ccatagtgaa cacacacctt tttttataat taaaaaattc gcctttttat tctactatga
    29941 atcttctggc ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa
    30001 aaaattaaac cattagcata atattttctg aatctattca cgaaagtctt tttctatctt
    30061 tcgttttcca cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg
    30121 acgcacaagc tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc
    30181 cagttaactt tctgttctta gctttttact caactggata atatatgcac ttaatgaagt
    30241 cccaatttcc gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc
    30301 gcctacgtcg tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag
    30361 agtctgaagt gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa
    30421 tatctcaaat attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc
    30481 ttttcccatt tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc
    30541 tgttttagga tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc
    30601 aacagcggaa gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc
    30661 atctttcatc aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt
    30721 tgcttgaagc acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt
    30781 gacttgagtc gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt
    30841 gttttccaat tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt
    30901 tacgatcaag atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt
    30961 tggtttatcc acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg
    31021 tgttcctagc ccagcggcag gaatgactgc ttttttgacc ttcacttgct
//
LOCUS       DAYYAO010000028        30915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000028 DAYYAO010000000
VERSION     DAYYAO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.029"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(306..2381)
                     /gene="glyS"
                     /locus_tag="ACHARI_001175"
     CDS             complement(306..2381)
                     /gene="glyS"
                     /locus_tag="ACHARI_001175"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8442232.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2383..3300)
                     /gene="glyQ"
                     /locus_tag="ACHARI_001176"
     CDS             complement(2383..3300)
                     /gene="glyQ"
                     /locus_tag="ACHARI_001176"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8442233.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3684..4496)
                     /gene="recO"
                     /locus_tag="ACHARI_001177"
     CDS             complement(3684..4496)
                     /gene="recO"
                     /locus_tag="ACHARI_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8442234.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4639..5538)
                     /gene="era"
                     /locus_tag="ACHARI_001178"
     CDS             complement(4639..5538)
                     /gene="era"
                     /locus_tag="ACHARI_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8442235.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5553..5948)
                     /locus_tag="ACHARI_001179"
     CDS             complement(5553..5948)
                     /locus_tag="ACHARI_001179"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8442236.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5932..6408)
                     /gene="ybeY"
                     /locus_tag="ACHARI_001180"
     CDS             complement(5932..6408)
                     /gene="ybeY"
                     /locus_tag="ACHARI_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8442237.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6425..8617)
                     /locus_tag="ACHARI_001181"
     CDS             complement(6425..8617)
                     /locus_tag="ACHARI_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8442238.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8641..9612)
                     /locus_tag="ACHARI_001182"
     CDS             complement(8641..9612)
                     /locus_tag="ACHARI_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8442239.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10318..11697
                     /gene="brnQ"
                     /locus_tag="ACHARI_001183"
     CDS             10318..11697
                     /gene="brnQ"
                     /locus_tag="ACHARI_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8442240.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11853..12299)
                     /locus_tag="ACHARI_001184"
     CDS             complement(11853..12299)
                     /locus_tag="ACHARI_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8442241.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12326..12502)
                     /gene="rpsU"
                     /locus_tag="ACHARI_001185"
     CDS             complement(12326..12502)
                     /gene="rpsU"
                     /locus_tag="ACHARI_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8442242.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12664..13092)
                     /locus_tag="ACHARI_001186"
     CDS             complement(12664..13092)
                     /locus_tag="ACHARI_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8442243.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13184..14044
                     /locus_tag="ACHARI_001187"
     CDS             13184..14044
                     /locus_tag="ACHARI_001187"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8442244.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14128..14478)
                     /locus_tag="ACHARI_001188"
     CDS             complement(14128..14478)
                     /locus_tag="ACHARI_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8442245.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14494..16221)
                     /locus_tag="ACHARI_001189"
     CDS             complement(14494..16221)
                     /locus_tag="ACHARI_001189"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8442246.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16434..17360)
                     /gene="trxB"
                     /locus_tag="ACHARI_001190"
     CDS             complement(16434..17360)
                     /gene="trxB"
                     /locus_tag="ACHARI_001190"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8442247.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17505..18938)
                     /locus_tag="ACHARI_001191"
     CDS             complement(17505..18938)
                     /locus_tag="ACHARI_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8442248.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18938..20341)
                     /locus_tag="ACHARI_001192"
     CDS             complement(18938..20341)
                     /locus_tag="ACHARI_001192"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8442249.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20571..21473
                     /locus_tag="ACHARI_001193"
     CDS             20571..21473
                     /locus_tag="ACHARI_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8442250.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21514..23400)
                     /locus_tag="ACHARI_001194"
     CDS             complement(21514..23400)
                     /locus_tag="ACHARI_001194"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8442251.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23497..24348)
                     /gene="licT"
                     /locus_tag="ACHARI_001195"
     CDS             complement(23497..24348)
                     /gene="licT"
                     /locus_tag="ACHARI_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8442252.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24806..26590)
                     /locus_tag="ACHARI_001196"
     CDS             complement(24806..26590)
                     /locus_tag="ACHARI_001196"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442253.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26590..28341)
                     /locus_tag="ACHARI_001197"
     CDS             complement(26590..28341)
                     /locus_tag="ACHARI_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8442254.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28483..28707)
                     /locus_tag="ACHARI_001198"
     CDS             complement(28483..28707)
                     /locus_tag="ACHARI_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8442255.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28958..30544
                     /locus_tag="ACHARI_001199"
     CDS             28958..30544
                     /locus_tag="ACHARI_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8442256.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8638 a         6637 c         5510 g        10130 t
ORIGIN      
        1 atgattctaa gatcattttg aattaatcag tcttatagtt gatttctaaa ttgtcatata
       61 ttattaaaaa tttttgccat ttaaaaagaa aagcgacagt ttaccaataa atttcaactg
      121 aattatctaa aagtagatca gttagtttag ataattcagt tgaaattgac cgactcaaat
      181 tacttgtata atacctagga aaaaattaac tagcaccacg ggtatagtta tttatattta
      241 taacaaataa accagagaaa tagcctattt tgtgcctatt ctctggttta tttacatatt
      301 agaaattatt ttgtgatgat ttgatctaaa cttgctagcg acaaagccat ggcagcaatt
      361 tgacttaatt gcgttaatcg gttcttacgt actgctggtt cttctgccat gaccatcgtc
      421 tgatcaaaat aattttcgat cagtgggcga aggcttacaa gctgatcata attttcgttg
      481 atcgtttggt cagcaaagct ttttttcatt gcctctactg ctttgtgcag ttctttctcc
      541 gcttcatttt cgaacagttc agggtctact tctgattgtt tttcaagctc cgcttttttc
      601 gctaagttca cgacacgtgt caaagcttcc atcgatggtt tgaagtcttc atctgcaaga
      661 tgctgcttga acaattgtgc tgctgcaaat acttttgtca gatctttttg ttctgcattc
      721 aaaacagctt cgatgacatc atgacgaata ttcttcgttg tcaataattg gcgcagacgg
      781 cctttgataa agtcgatcac ctgttgttgc ccttctgata aaccaatgcc gaaacgatcc
      841 gtatctttac tgataacatc actaatcgtt tcttgcagaa cagacaaagg aaaggcccat
      901 tttttcgatt cgatgatccg aacgacaccg tatgcttggc gacgtaaggc ataaggatcg
      961 tttgacccgc ttggaatcat cccaactgca aagaaagaaa aaatgctatc taatttatct
     1021 gcaatcgcta aaacagaacc tactgtagac tgaggtagct ctccttcact ggatataggc
     1081 atgtaatgct cacgaatcgc tgttgctaca gcaggtttct cgcctttcag cagagcatat
     1141 ttttcgccca tgatcccttg tagttcagga aattccccta ccatgttggt tactagatca
     1201 aatttataga tttcagcagc tcgttgcaga tccatcaatt cttcctcaga tagtcctact
     1261 tgttcgccaa tgatacgggc aatgacttgt acccgctgca ttttctcata gatactaccg
     1321 attttttcat ggaaagtaac agtttttagt ttttcaacgt attggtcgat tgaagctttc
     1381 ttgtcttcat tataaaagaa ttccgcatct tctaaacgag ccgtcaatac tttttgattg
     1441 cctcgaacca cattttccaa ttttacccga tcaccattac gagcagaaat aaaatgagga
     1501 agcagcatac cttgatcgtt ccgtacctcg aaataacgtt gatgttcttt catagaagtg
     1561 actagtactt cttcaggaac agaaagatat ttttcttcga agtcgccaga aaagacagtt
     1621 ggatattcga ctaaattgtt gacttcttct agtaaatctt cgtctagatc aataacccag
     1681 tgattttttt cggctaattc atttgcttgt gtaacgatca tttcttgtct tttttgcggt
     1741 gaagcaataa cgaattgttc attcaatttt tcttcatatt cttttgcatg cgcaaatgtc
     1801 acatcttcgc ctaagaaacg atgtcctcga gaggctctgc ctgttttgac atctaagatc
     1861 tcaaacggga ttacttcgtc atctaacaat gccacgatcc agtgaatagg gcggatatat
     1921 tcaaaatcgt attctgccca atgcatcgtt actgggaaag tgaggctttt aatgacatct
     1981 gctaaacctg tcaatacttc tttcgcttct ttgccgtgag tgtatttcgt tacatagaca
     2041 tattccacgc cttttaattc tctaaaagtg atagcatctg tcgtcagacc ttgtccgcgg
     2101 acaaatcctt cagctgcttt cgtccagttt ccatctgcgt ccaaagcaat ttttttggct
     2161 ggtcctttga cttcttcttc actgtctgct tgacgttctg gaatgtttgt tactttgatt
     2221 gctaagcggc gcggtgtaga aaacgtttcg atcgtatcat aagtcaattg gttttcatct
     2281 aaaaattttg ctgttttttc ttctaattgt ttcatgcttg gtgtgacaac atgagcaggc
     2341 atttcttcca gcccgatttc aagtaataag tcttttgcca tcttattcag cctccttcat
     2401 gttttctgga tttgctgtgt ttttattcaa taaagggaat cctaattttt cgcgttctgc
     2461 cacaaagatt ttggctacag cacgagccat gttgcggata cgggaaagat aacctgctcg
     2521 ttctgttacg gatactgcac cgcgagcatc taataaattg aatgtatggc tgcatttcaa
     2581 aatataatca tacgctggat gaacaagatt ttcatcgata cagcgtttag cttctttttc
     2641 aaaacggtca aagttgctta gtagcaagtc ttggtcactt acttcaaaag aatatttgga
     2701 atgttcatat tctggttgtt tgaagatctc gccatatttc acaccatcag cccattccag
     2761 atcatagaca ctttcaactt cttgaatata tgaagcaaga cgttctagtc cataagtgat
     2821 ctcagaagta actggtttac aaggtagtcc tccgacttgc tggaagtacg tgaattgtgt
     2881 gatctccatc ccatcaagcc atacttccca acccacaccg gcacacccca tcgatgggtt
     2941 ctcccagttg tcttcaacaa aacgaatatc gtgttctaat ggatcaattc ctaaaagacg
     3001 caagctttct aaataaagct cttggatatt ttcaggtgaa ggtttcatta cgacttgaaa
     3061 ttgatgatgc tgatacaaac ggtttgggtt ttctccataa cgcccgtcag ctggtcggcg
     3121 ggatggctct acataagcag cattccatgg ttccggtccg attgcccgta agaatgtata
     3181 tgggctcatc gtacctgctc ctttttctgt gtcataagct tgcatcagca tgcagccgtt
     3241 tgatgaccaa aatttttgta atgttaaaat catatcttgg acggtcaatt ttttactcat
     3301 ctgttttgcc cctttcaatt tttaagcgtt gctgtcaata agaatacagg aaagaaaacg
     3361 acaaaaagtc cctatgtctg attacagaca tagggacgaa taatttcgcg gttccaccct
     3421 acttccgaat aaagattcgg cagcttcatt tatcgtactc gcaagtgcct cttcaaaaat
     3481 cgtttgggtc cggcttccac tctcccgaac tcgctaaacc acgtgttttt tactgatctt
     3541 ggtcaatgta cgtattcatt ttgcagtttt cttgtttata taatagatcc tctcagtgaa
     3601 cgatctattt aatctacggt taataataga cggaagtccc tgtatttgtc aaatagtttt
     3661 gatttttttg ttgtatcttt catttattct tctttttcag gtatctcttt ttcaggcata
     3721 tttttctctg gcgtctttaa tacgtgtgcc caagacttca tttgatcaat gaactttttg
     3781 ctttttagat gaataccgac atattcatca tacaactggt caatgaggtc acggattgcc
     3841 tgcttcgttt cttctttcaa gtggattcca ctgatcttat cataagaaat agccgaaaac
     3901 attcgaacga aataaacagc gcgaggatct gcatgataac ggtgttcgtc cattggccag
     3961 tgtttctcgc atatcacacc gccagatttg gaagaatagt caaattttcc ttttgtttcc
     4021 ccgcatacga cacagtgcgt ccaaattggc gcgattccga agcgttgcag tatttgaact
     4081 tcaaagatat tcgtgatgat ttctgcatca tctccttgat ccatccgctg cagagcttgt
     4141 tgcgtaaatt ggaataaatg cgggtcatat tgatgatctt cgattgctgc atctactaga
     4201 tttagcaaat aggtaccata accgctcaaa aagatatctt cttggatgtt ccgaaacgca
     4261 tggacgtctt tgacactgct taaaaacgac aaaccttcac ttcgaaaatt accgatatag
     4321 actgcttcgg taaaggggag caaagccgga gctaatggct gattttgccg atggattcct
     4381 ttgatataaa acatcatttt gccagaagat tccgtaaaga ttttgactaa tttatctttt
     4441 tctttgtaat tacggctaga taaaatcaag cctttcgatt ctgcaagctg gctcatattc
     4501 tccctcctaa aaaataaatc tggaaaagca gattctgccg atccagatta tttatgtttc
     4561 gatcttccgc cgtttttctc aacgtgcgga tttttatcca gtttatttct atacttaacc
     4621 tataagaagt tcagaatatc agtattcgtc ttttcggtag ccaaagtctt gcagatagac
     4681 ttttttgtct cgccagtctt tttggacttt gacccaaagt tctagaaata ctttgtctcc
     4741 taaaagattt tcgatatcct ttcttgcttt cgttcctact tgcttcagca tttttccgcc
     4801 tttgccgata atgattcctt tttggctatc tctttcgacg atgatcgtgg cctggatatg
     4861 gattttatca ttctcgtttc gcttcatcga atcaacaacg actgcgacag aatgaggcac
     4921 ttcgtctcgt gttaaaaaca agactttctc acggatcaat tcagaaacga taaagtattc
     4981 aggatgatca gtcacttgat catctggaaa atattgtggg ccctctggca tttcactgac
     5041 taatacatcc attaagcgtt cgacattgtt tccttctgta gcagagatcg gaatcacttg
     5101 tgcaaaaggc atctgcgtcg aataatcttc gatgattccc agcaactcgt ctggatggat
     5161 cttatcgatt ttattgataa tcagataaac tggagaatgc acgtttttta gacgttcaat
     5221 aataaaatcg tctccccggc cgcgtttttg atcagcactg atcataaata agactgcatc
     5281 cacttctttc aacgcactgt aagctgcttc taccataaaa tctcctagac gatgctttgg
     5341 tttatggatt cctggtgtat cgataaaaat cagctgagct tctggtgtag tatatactcc
     5401 ttggatttta tttctagttg tctgtgcctt gtcactcata atggcaattt tttgacccac
     5461 gattcggttc aataatgtgg attttcctac attcgggcgg ccgacgattg cgacaaaccc
     5521 tgatttatgt tctggcatat acgttctcct ttttaattaa acaacaaatg atatatttta
     5581 gggacaaata aaatcaaccc aatgatgaca gcaaaacaag cggtcactaa aactgctcca
     5641 gctgccatat ctttgatttt tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc
     5701 actacatttt caaaaacagt attgatgatc tctactatcc aaacgagaaa aacagctaaa
     5761 agcagccata accactctat ccgatcaagc tggaaaatca cgcccataaa taaagcaagg
     5821 ataccgaacg caacatgttt cctcatattg cgttcttcct caaaaacggt tttgactcct
     5881 tggacagcaa attccacaga catcaggaaa tgtttgtttt tctctacgtt tttatctttt
     5941 gagtccatag gcatccaaga tctctttctg taaaccaaac atttctttct catcttctgg
     6001 tgtcatatgg tcatacccgt tgatatgcaa gaatccatgt accgctaaaa agccaagttc
     6061 ccgatcatat gaatggccgt aagcttctgc ttgttcagca gcacgttcaa tagaaatcat
     6121 gatgtctcct agtacacgag gcaaaggatt ctctccatct tcaaaaatca ctgggatttc
     6181 gtcttcgcct tcttcttcca gagcaaaact gatgacgtct gtcggtgcat cttttccacg
     6241 gtaatcacga ttgattactt ggattgccgc attgtccatg aaagtaacag acatttctgt
     6301 ttcctctggc agctttaaat aagatgctgc aaattgcagc aagtcatcga tttctttcat
     6361 tttctcttct gagacttggt ttgtttcatc cataaaggta atatccatta tacatcgctt
     6421 ctttctattc ttcttgttct tcctttatct tctcaacttc tgttttcatt ttaggatatt
     6481 tgatccgaga atggaaggtg ctacttaaac cgctgatcaa tgatttttcc acttttcgga
     6541 tttctttcaa ggtcagtcca ctatcgtcta attgaccatc ggaaatacgc tcttcgataa
     6601 gattatggac aaaactttcg attttttcgc tcgtcggatg atccattgcc cgtactgcag
     6661 cctcacaact gtcagcaata ttgacgatcc ctgcttcacg actttgcggt tttggtccag
     6721 gataacggaa ttgctcttct gttacttccg gattgcgttc tttcgctttg atgtagaaat
     6781 aacgcattag cgtggttcca tgatgctgtt tgcaaatatc gatcaccatt tgcggcatat
     6841 tataatcttc caatattttg gctccatctg tcacatgtcc aaagataata tgtttactgt
     6901 cttctggcaa taagaaattg tgcgggtttt ccgctcctga aggcaagttc tcaacgaaaa
     6961 agctagcatg tttgatcttg ccgatatcat gataatagca agctactcga gttagcaaag
     7021 aacgtccgcc gatttctgct acagcgttag cacttagatt agctaccatc atactatgat
     7081 gataagtacc tggcgcttct tccagcaact gtttcaataa aggatgattt ggattgctga
     7141 gttcatttaa tacaattaca ctgtcatcat tgaccatcaa ttcaatatat gggtgtagac
     7201 cgatcgtcag caagaatgag aagagtgcac cagtgaaccc gcagacaagc attaaccaag
     7261 tcgttgcatc accaaagctc attccttgat agacgatcaa aatgacatcc atcatcacag
     7321 gaaaaataat gacccacatg gcagcagata atccttgttc tgagatccgt ttgcgtctga
     7381 caatcgttgc catgagccca gagaaaacat aagacatcaa gataatcgtt agaaagttcg
     7441 tgccaatcga atcataaaaa atgaaaagtg cagatactgc ttggaaaata ccagccatga
     7501 tccctgctct tcggttcaag aaaaagctta aaacaagtgg tgcaaaagca gcgggataaa
     7561 acagcgggat ataagtagct tgttccgttt ggaataattg gaaaaatttc atcagcacta
     7621 tgctgactga catagctgtc acatagaaaa gcacgtattt tgttctttct cctgcatttt
     7681 ggaattgaag tgaattataa atcagtacgc cgatttgaag caaaacagct aaaaccatgg
     7741 caatgagtgg gaaaatcgaa gtcgtttgat tcgtcaatcc tagtaattcc aattttttca
     7801 tcgctgaagc atcaatttga tttccttcgc gaacaatgat ttctccttgg aagatcatca
     7861 ctggttgtac agcttctcgt gcagattgtt ttaattcttc tgtctttttc tcattcaata
     7921 catcattgac tacaatgcct tgatccacta agtagcgaat catttgttgc tgctctgttg
     7981 tgacattcaa atactgaatc tgatcttctg cttcttgttt aaaagcttcc aattcgcttt
     8041 cacgaacttg tttactcatc tgttcgtcaa tgagcttcaa actttcatcc ctaaccgtgt
     8101 gaatttccgt ttcagtcatt tcaaatatcg tcgaataaaa attgttcggc agtttttggt
     8161 aaaacgcaac gttttctgca ttcacgtttt caaaattttt cttcagtgta gcaatccgct
     8221 cttctgtagt aggtttcggg atattttcat cttccttcgc cttatctacc ttgtcattat
     8281 aagatttgtt gatggtatca ttcgtcttat cgatcaattc gaataattgc ttgatccgat
     8341 tgttctggtc atctgccaga tctttttgat aagtatattc tggagtcact gcttctgctg
     8401 ccagttttct tctttgttcg gtttcttccg tgttctcgat cgttttattc gcacggatac
     8461 tctcttctgc tacttggcct tctctgaaat cattggcctt ttgtttgaca ctactaaatg
     8521 taacgccgca gacaatgata aaaaacaaga acagaaggaa aggcaatgct gcctttccga
     8581 gtttttcttg tagtttctgc agtgttttat tcaacatgtg cttatcctcc taagcaaagc
     8641 ttattcttga tgaagatccg ccttttcata agctttgatg atttcggcaa ctacagggtg
     8701 ccgaacaacg tcacttgctt caaaattaac aaaatcgatt tttttgattt gctttagtgt
     8761 tcgttctgca tgaacaagac cacttgtcgt ccctcgaggc aagtctatct gacttgtatc
     8821 cccattgaca atcatttttg attgataacc gagacgggtc aagaacattt tcatctgagc
     8881 gactgtcgtg ttttgtgctt cgtccaagat aacgaatgca tcatccaatg tccgcccccg
     8941 catgtaagct aatggtgcga tttcgattac gcctcgttcc atcaatcggt tcgtatgatc
     9001 caatccaaaa atttggtata aagcatcata aacaggccgc agataaggat ccactttttc
     9061 tttcagatcc ccgggtagaa agcctaggtt ttcccctgcc tctacagcgg gccgcgtcaa
     9121 aatgattttt tgcacttcgc ctttttttag ggcagcaatt gctaatacta cggcaagaaa
     9181 cgttttccct gttccggctg gccctacacc aaatacgatg tcatgtttgg ctaccgattc
     9241 aacgtatttc ttttgaccgc tgttttttac ccgtataggt tttccattac ggtctttgac
     9301 gatctcttcc tcatacatct caacaaaata atctaatgta cctttttgcc ccattttcaa
     9361 tgccgtcacg acatctggtg tacttacgtg gattcctcgt ttgatcaatt cctgtaatgt
     9421 ccgaatgact gaggcagcca aggaaatctg ggattgttca ccaatgatct gaatgatttc
     9481 tccacgtgta tggatggttg tttccgttgt ttcctcgatc agttttatat gcttgtcgtg
     9541 tgcacctaaa agcatgctga catcatcagc agctgtcaat ctaatctcta aagaactaga
     9601 ctcttccgtc aaaaatagac atcccctttt tttcgattca ttctttccta ttttaccata
     9661 ctcagaagaa actatatagc aaataagaaa aggttgtaaa agagtcgttt aaccatacgt
     9721 accaagaaac gttaaaaaat agctgtttcc attcgctaaa ggtctcgttt tgataaaaag
     9781 aatctgagaa tgtaagttat atgaaagtgg agaacaaaat attccgtttc attcagaggt
     9841 attctctagg tatttttctt gtttcaaaaa aagaaaacaa aatagttaaa atattcttgc
     9901 atttcaaaaa aaacaggtgt attattacgt caacaaataa acatacagca aagcgtcgag
     9961 gaaaacagta ggctattcaa cgatttattc ccagtgagtc atgtaagtga gaaatgacaa
    10021 taaatcgaat agacgaacat catttccgag tcaatgtcga aaacgtagat cattgtggct
    10081 gcctccatta tcaggctagc ggataacttt atcaaaaagc gtccgtgaat gaagaagctt
    10141 ttttaagcga aaaaaggtgg taccgttcta gaatctagag cccttttccc aagtggaaaa
    10201 gggctttttt ttatatttta aggtggtggt tttcatggaa tctgatatta gtggcattac
    10261 taattgctgc aacgtttata tcgtcaatca aaatgataat tattggagga aaaatttatg
    10321 gaagaaacaa aaacttattc atggaaacaa ctattgatgg tcagttcact tatcttcggg
    10381 atgtttttcg ggtcaggtaa tctaatcttt cctgtccact taggtcaaat ggctggcgca
    10441 aactgggtca gtgctggcgt aggatttgcc atctcaggcg ctcttttccc attactagct
    10501 atcttagctg tagtagtgac aaaaagtgat ggtctgtatg acttagctaa acctgtcgga
    10561 aaatggtatg ctgccttgtt cttaatcctt gttcatttga cgatcggacc atttttcggt
    10621 acaccacgga cagctgctac tgcttttgaa atggctgcta aaccattttt gcctgaaaaa
    10681 tataattcat taggtatgtt tttattctct gctcttttct ttttacttgc ttatttcctt
    10741 actgtccatc ccacacggtt ggtcaaatac gtcggaaagt atctaaactc cttgtttctt
    10801 gcattattag caatcatttt tgtcgtttct ttattccacc caatgggaaa tctaaatcaa
    10861 tctgctgcag atacttacca aacaaacgct ttaacaaatg gtgtcctaga aggttataac
    10921 acggttgatg cagtagcctt gcttgcctta agtgtcactt ttgtccatgc agtccgtgac
    10981 cttggtttca aagataaaaa agtcacagaa ttaacagcaa aagctggtag cctgagcgtt
    11041 gcgctagaag tcatgatcta ttttggtctt gttcttctcg gtgcgatgag tttgaataag
    11101 atgtctttat cagaaaatgg cggaactgca ctttctcaga tcactaattt ttatttaggt
    11161 aacttcggcg ctagcttctt aggagtgatg gtcacattag gtgtcttcac taccgctatg
    11221 ggactcgttg tttcttttgc acaagatttc cataaactgt tccctaaagt cagttatatg
    11281 acatggctgc ggttaacgac tttcgtctct tttgtcgttg caaatgctgg tttggacaat
    11341 atcatccaat ggtctttacc agtattgatg cttttgtatc cattgtcgct tgcgttgatt
    11401 ttgctttcct taacagcaaa atttttccaa aagacaccat ttgtctatca agtaacgatg
    11461 ttgtttgctg ctgttcccgc cgtattggat atgctggcaa attcgcctgc tttagtgagt
    11521 caacaaagag ttgtagcaag catgctcgaa ttttatcatc atcacgttcc ttttgctgca
    11581 ttgggacttg gctggatggt cccaacgtta cttgggtatg ctggcagctt actcttctat
    11641 tacgcctatc gcctatcagg gtataaacaa gaagcaaatg aactaccaga agaataacca
    11701 gatcaaacac aatttaacat aagaaatatt aaacaggaaa aaaacagcta gatgaaagaa
    11761 tcctattctt tcgtctagct attttttaga tctctttttt acgagcacaa aaaaactgcg
    11821 gacaatgatt gtccgcagtt taaatgataa aattaagatt gtaatagttc tttcacaatg
    11881 gcatttactt ggtttccatc tgcttttcct ttgactttag gcatgatcgc acccatgact
    11941 ttgccaaatt ctttcattga tgaagcacca gtttgcgtga ttgcttcttg aacaagttgt
    12001 cgaatttctt cgtcggaaag ttgttccggc atgtattttt cgactatcac aatttcactt
    12061 tttacttttt cagctaaatc gtcacgtcct gcttcttcaa actcatgcaa agaatcacgt
    12121 cgctgtttca tttcgcgaga caacaccgtt aattcttctt cttctgtcaa gtcgtgacct
    12181 tctttaatct gctcgttttg tatagatgac ttgatcattc ggatgacttg taaagattcc
    12241 ttatctttcg ctttcatcgc tgttttcatg tcatcgttta atctacttag tagtgacaca
    12301 ctaccaactc caattcatca cagaactaga attttttgcg ttttctagct gcttcagatt
    12361 ttttcttgcg tcttacactt ggtttttcgt agaattcacg tttacgagat tcttgtaaag
    12421 tacccgcttt tgacacggaa cgtttgaagc gacgaagagc atcgtcaagt gattcgtttt
    12481 tgcgaacgac tgtttttgac atgttaattc cctccctccg agcttaaaaa gtaaccgtaa
    12541 ttgttattga aatatatctc aaaaaacaag actgtcctgt aaatattata tctaatgctg
    12601 ttttgaccgt caaggaaatc ttgctttttt ttaaaattat caaatgttat tttatttaaa
    12661 tccttattga caattttcac atcttccgaa aatctcgaat ctatgccctt cgatcgtaca
    12721 tccaccgagc tgttcttcga aataattcat tggacacata tggatttctt tcgttgttcc
    12781 gcacaccgta cagataaagt gatggtgatg gccgatttcc ccttgacagc aatgaaaacg
    12841 aaacttcatc tctccattta aatcggtttc ttccagtaaa tcgatttctg caaaatcatg
    12901 gagatttcga taaatcgtat cgtaacttac tcctttgaat tcctgattca tatagtcaaa
    12961 aacttcttta gcggatacgt agcgattgcg cttcactaaa taagttaaca atgcttctct
    13021 ttttttagta tatttaaaac cgttattttt caatgtttct agtgattgtt cgatcaacgt
    13081 ctgttttttc atgacttatt cctccaaatc aaaatgatta cgaagtacac attctagagt
    13141 atggtataat aaacataaaa aaaatgcaag tgaggtttta tccatgtctt ccgattctac
    13201 agaaatcgtt actttttttg tcatctccga ctctgctgga gaaactgcga caaaactagc
    13261 gcaagccact atggcccaat atcctagcgt tgagttcaat ttattccgca gaacatttgt
    13321 gacagatgag aaaacattga tgcaggcact gcaagatgca ctagaagaaa aagctcttgt
    13381 cctgcacaca ttgatcaatc aagaattgat cgagatgacg cgttcttttt gtgaagaaca
    13441 tcaattattt agttttgatg tgatgactcc gccgatatca gaaatcgagc gcttaactgg
    13501 tatcaagccc attcggcaac ctggcgccct tcatttattg aatgagaact atttcaaacg
    13561 gatcaaagca atggaatttg ctgtaaaata cgatgacggg aaagatcctc gtgggtttct
    13621 agaagcagat gtcgttttgc taggcgtatc ccgaacttcc aaaacgcctt tgagcttgtt
    13681 tttagctaac aaaaatttaa aagttgccaa tctacctttg atcccacaag cacatattcc
    13741 caaacagctt tgggagatcg atcctaaaaa aatcgtggga ctgacgaata acccagatat
    13801 tttaaacaat atccgtaaag aacggatgcg ctcttacgga ttaaatcctg atactgctta
    13861 ttccgatatc gaaaaaatac gagctgaact cgattttgcg aatgatcttt atgaaaaatt
    13921 gggctgcgta gtaatcaatg tcgcttcttt atctattgaa gaaacagctt cgcttatttt
    13981 gaatgcttta gacctagaag accatagtta ttatggaact gaaaccaccg aagaaacaga
    14041 ctaacaattt ttctttaaaa aaactggact gtgtcagcga agaagattcg ttgacacagt
    14101 ccagtttgtc tctatttgca cctatcgtta ttgatgttcc acatgtttga gtgtctggtt
    14161 caaaatatcc agtacgtgag aatcatcaag actatacaaa atcgcttttc cctcacgatg
    14221 ggacttgacg acatggtttt ctcttaataa acgcagttga tgcgatacag cagattgttc
    14281 catttccact gcttggatga ttgctgtcac attttgttcc ccgtctttca gatacagcag
    14341 aattttgaga cgagtggggt cactcaacat tttgaatagt tggctgactt tctctacttt
    14401 tgttttttgc gtgtctggca atccattcac ctccaacttg aaaaaagact gacttctaaa
    14461 gtttaccact ttttccatca gtctcaagtt tttttattct tctgtcatct tttggataat
    14521 cgtttcataa gcggctactt tttcagctgc gtcttctgaa gagtttcctt ttacaccgat
    14581 ataaaatttg attttcggct cagttcctga aggacggata gcgatccagc cattttcttc
    14641 cagcgtatac ttcaatacgt cagatggcgg tgtcgttagt ttttctacgt ttccttcacg
    14701 gtctgttctc gtcaactctt tgaagtcttc tgtttggatg acttttgctc cggcaaattc
    14761 tgttggtgct tcttcacgga atttttccat caaagcagag attttcgcag aaccttcttg
    14821 tccgctcatt gtcacagaaa tcgttttttc tgcgtagtag ccataggttt caaaaatcgt
    14881 ttgcaagcca tcatacaacg ttttgccttg ttttttatag taagctgcta cttctgctaa
    14941 taagagcaat gcttggatcg cgtctttgtc gcgaacgaaa ggtttaacta aataaccgta
    15001 actttcttcg aatccgaaca taaaagtatg ggaatgatct tcttcaaatt gttgaatttt
    15061 ttcagcaatg aatttgaatc ctgtcaatac attgaacatg gtagccccat agtgttccgc
    15121 aattgctgta ggtaattcgc tagaaacgat cgatttcaat accgctgcgt tttcaggtaa
    15181 agtgcctcgc gattggtgtg cttccaaaat atattgagca aacagtgctc cgatttggtt
    15241 tccggtcaat acttcatagg agccatttgg caaacgaacg gctgcaccta aacggtctgc
    15301 atcaggatct gttgcaatca gcaaatcagc gccttctttc tcgccaagtc gtatcgcata
    15361 ctcaaaagca gagtgttctt ctggatttgg tgatttgact gtagtgaaat ctgggtctgc
    15421 gacagcttgt tctggaacta gtacgaattg ttcgaatcca gcttgtttca acgcacgttc
    15481 gcccaacatt tttcctgttc cgtgaagtgg tgtgtacaca agtttcaaat ctttccccat
    15541 tgtttccacc aattcatgat caatcgttac tgctttgatt tcttctagat aagcggcatc
    15601 cacttcttct ccgatgatcg tgatcaatcc gctatgttca gcttcttcgt cagataatac
    15661 ttcaactttt aacggattgc tgacttcacg gacatatttc gtcaatgcat ctgcgtctgc
    15721 aggaggcatt tgtcccccat cttctccgta gactttatat ccgttgtaag ctgctggatt
    15781 gtgggatgcg gttaccatga ttcccgcaaa cgtcttcaaa tgacggactg caaaagaaag
    15841 ctctggtgtt ggacgtagac tttcgaatac ataagatggg atattgtgat gtgctaatgt
    15901 tttggctgct tccatggcaa attctggtga ttgatggcgt gagtcataag cgatagcgac
    15961 acctcggcgt tttgtttctt cgccttgtgt atccatgaaa cgtgcaagtc cttctgttgc
    16021 ttgtcttacc gtatagatgt tcattcgatt gatcccaggc cctaagattc cccgcatgcc
    16081 tgcagtaccg aattccaacg gtgcataaaa agcatcttca agtttttcag gatcttcacc
    16141 cagatcattt aattgttttt ttaagttttc ttctaaattt tcttcatgta cccattgttc
    16201 atacacttgt tcccaagaca tgaaaataac acctctttct gaaatatggt tcttaataag
    16261 tatagcattc acttattaga tatgaaagta tgaggaccaa aaaacgctta aatcaagaaa
    16321 ggattctttt atcaagcacg gttgcaagta ttcgcataaa tcggattcag tagatttcac
    16381 gcgaaaaaaa agagtaaaag aacaattaaa ccgttcttcc actcttttta aacttatgca
    16441 gttgtttctt ctaaagattc caagtattta tacacttgtt gtccagcgat cccaccatca
    16501 ccaacagcag tagtgatttg tcgcaaatct ttttcacgaa catctccgat ggcaaaaacg
    16561 cctggtatat tcgtcttcat ttcgttgtct gttggaatcc agccagcttc gttcgtcaac
    16621 cctgcttctc taaatggtgc agttaatgga tccagtccaa catagatgaa tactccgtct
    16681 gctgccaaat caaactcatt gccggttttg acattacgtg ctttcacacc tgtcactacc
    16741 atttcattcc caacgatttc gtcaacgact gtatcccaca taaaggaaat tttttcattc
    16801 gcaaatgcac ggtcttggat gatcttttgt gcacgtaatt catctctacg gtggacgatg
    16861 actacttcag aagcaaattg tgtcaaatag atggcttctt ctactgctga gtctcctcct
    16921 ccgatcacta ctagacgttt gttacggaaa aacgcaccat cacaaactgc acagtaagaa
    16981 acgccacgtc cagcaaattc ttcctcgcca ggtacgccta atttacggtg gatacagcct
    17041 gttgcgataa tgactgtttt tccttcgtaa gttttatctt cacaaataac ttctttgtgg
    17101 ctgccgtgat ccttgatatc ttgcacgatt ccataagcat tttctgtccc aaatcttgaa
    17161 acaccttcat acatcttata agctagatca ggacccatta ttgaatcgaa accaggatag
    17221 ttttcgattt ctgccgtatt gttcatttgg ccgccagggg cgccttgttc gatcatcaat
    17281 acggataagt ttgaacgtga agcatataaa gcagcagtca tcccagcagg acctgcgcca
    17341 ataacgatta catcatacat atgtagattc tcctttactt aatcaagtaa gcatacttta
    17401 cagccaatgc gaaaaaaagt ctattgttct gcttgcgttt ctcgaatagc tttattatac
    17461 tcgaaaaatc cgctgaaaaa caatagactt ttataaatca ttgatcaatc attcgaaaga
    17521 ttttctccat ttgaatcaat tactttttta taccagttga atgatttctt cttcgaacgg
    17581 gctaatgtgc catttccttc gttgtcacga tcgacataaa tgaatccgta ccttttcttc
    17641 atctcacctg taccagctga tactaaatca atacatcccc aagtcgtata gcctaataga
    17701 tcgactccat ctaattctac tgcatctttc attgcctgga tatgtgctgc cagatagtcg
    17761 attcgatagt catcttccac ataaccattc tcatcgggcg tatcgactgc acctaagccg
    17821 ttttctacaa taaatagtgg tttttgataa cgatcataaa tatcattcat cgttattctc
    17881 aagcctaatg gatcaatttg ccaaccccat tcacttgctt ccagatatgg atttttgaca
    17941 gaagcaaaga tatttcctgc agtctgttcc aagagttcgt tgtcagtagt cgaaactcga
    18001 gaagaataat aagaaaagga gataaaatca acagtatgtt ctttcagcaa tgcggcatcc
    18061 ccaggttcca ttttgatctg aatgccattt cgttccattt ctttcaatgc ataagcagga
    18121 tattctcctc gtgactggac atcaatgaag aaataatttt ctcgatctga ttttctagaa
    18181 gcccaaacat cttcaggatt gcaagtgtat ggataatatg ctcctgctgc cagcatacag
    18241 cctactttgt tctccggatc tacttcatga gcaatcttcg ttgcgatggc acttgcaacc
    18301 aactcatgat gtgccgcttg atatttgatt tgttcttcat tttctccttc ttcaaaatac
    18361 agccctgcgc ccatgaatgg tgcatgaagg atcatattga tctcattgaa tgtcaaccaa
    18421 tatttgacaa gtcctttata gcgattgaag atgactcggc acagattttc ataaaaacca
    18481 acgagttctc tggaacgcca cgcaccatat tctttgatca aatgcatcgg acaatcaaaa
    18541 tgtgtaatcg tcacaagcgg ctcgatcccg tacttttgac attctttaaa gacatcctca
    18601 taaaatttca aaccagattc atttggctct ttttcatcgc ccattggaaa aatacgactc
    18661 caagcaatgg acatccgata agtcttgaac cccatttctg caaataaagc aatatcttcc
    18721 ttataatgat gatacatatc gatcgcaatt tttgctggat aaaaatgatt ttcatcaaat
    18781 tcaaacattt tcttttttcc agtaatgaca ggaaaacggt cttctccgat tggcacaaca
    18841 tctacgttcg ctaacccacg tccgccttca tcataaccgc cctcgcattg attggctgct
    18901 gtagctccgc cccataaaaa atcttttctg aatcccatca tacttcctcc attacatgtg
    18961 tattgcttga taattctctt gccagttcat cttggacttc gattccttct gcttttaacc
    19021 ggctgattgt ttccttgaat tgcggtaaat actgctgatg tgcaataaac aactcattca
    19081 tgatccgttt tgctgtcttt cctgatcgta taagcggatt gatcgtaaac gcttgtaaag
    19141 ctgttccata atcaccagtc acagctgctt cgatcgtcag caattccata ttcttcatta
    19201 cttgcaacca gcctctttca gcagcaggaa gggaaccaaa cgctacattt cgtgcgcctt
    19261 gtgcgccaac ataggcagtg acttctacta cacaatctgc cggcagatca ggaacagctc
    19321 catcattctt tgtagatacc acgatctgtg tttctttatt tccatagatc gaagcaatcg
    19381 tttcacaggc agcatctgaa taataagcac cacctcgttt ttgcaattgt tcaggtttat
    19441 gatccaattc tggatctttg tataattcaa acagctctgc ttctgtttct ttcacttgct
    19501 gcgctcttgt accaatcgtt tgatattctt ctaaagcatg ttccagcatt tcttcttcac
    19561 ggtagtaata gcggtggtag ccacaaggga tcatgttcat ttgttttaat tgttctttga
    19621 aaaacgggat atcaaagata tttttcggca atccattgtc ttcatcgtat aaacgatcaa
    19681 tcaattctgc cgtgcggtca tttcctttat tatctgctac tttgtgccaa tggaagtgat
    19741 ttagaccggc aaacttgtag atcagctctt cttttttctt gccaagcatt gttggttctg
    19801 tcatcatcgc cattactggc acattgcata atcctatcac tttatcccat ttgccatatc
    19861 tgataactgc ttctgtcacc atcccgcttg ggttcgcgaa attgatcagc caagcatttg
    19921 gacataaacg cttcatgtct tcaacgattg ccaaaaccac tggaacggta cgaaaagctt
    19981 tgaatatccc gccagcacca tttgtttctt gcccaatcat cccataataa ctagggatcc
    20041 gctcgtcttt gattcgtgca tctaattgac cgacacgaaa ctgagtcgta acaaaatccg
    20101 catctttcaa tgcttcttcc cgatccagag ttgcatagat tttgacttca tacggagaag
    20161 catcccacat acgctgagac atctgactga cgatctcaag cttttctttt ccttcttcta
    20221 catctacgag ccagatttct cgtatcggta attcctcata tcttttgata aatccttcca
    20281 tcaactcggg ggtgtaactg ctgcctccac caatcgtagc gattttgatt ccttttgcca
    20341 tgttctcact cctttacttt ttacactttt attatctttt ttgtaaacaa aacaaacaat
    20401 agaaattcgt ttctttccag taacatttac aaaagttagt gttttctact ggatttttgt
    20461 aaatatcttg tcaaaaaatg tctttattaa gctatataac ataaatccgt ttacaaatca
    20521 ttttcaaaaa agttataatt tacgtaaaaa aacaagaaga aggtatctac atgttattac
    20581 gtgaaaaaat caaagaaatc tccttttctc cttcagaaaa tgaagtgatt cagtttctct
    20641 taagcgattc tccagcactt ggcgaaatga ccatccaaga gattgctgag cgatgttatg
    20701 tccacccgtc cacactcatc cgtgtagcaa aaaaaatggg ttttgatggc tgggtcagtt
    20761 ttcgtaatgc ttttttagct gaaacagcct atcttgaagg gaattttcaa gacgtagatg
    20821 cgaatcttcc tttttccacc acagacggta ttatgacgat cgcaaataaa atggcttctt
    20881 tagaacagat gacgattcaa gatacacttt ctctcattca tcatgaccaa ctgcaaaaag
    20941 ccaaacaact aatgcttcaa gcaaaaaaaa tcgtgatttt cgccagtaac tcgaatactt
    21001 tgatttctca agatttcatg ctgaaaatga agcgcatcaa aatggatgtc acgattgtca
    21061 ccacttttgg tgaatcgttc tatgaagcgt acaattgcac gagtgatatg tgcgggattc
    21121 tgatttctta tacgggtgaa aacaatgtga tccttcgtac cgcagaaata ttgaaacaaa
    21181 atcatgttcc aattttagct ttgaccagta tcggtgattc aacgctatct tcaatgagta
    21241 cagccgcttt gcgacttacg acacgcgaac gactgtattc gaaaattggt aattttacga
    21301 tcaatacatc gatttgttat cttctagacg tgttatatag ttgtgttttt gcagaaaatt
    21361 atcaacagaa tttagtccat ctaatttctg taggacaaaa atttgataaa cgccctatca
    21421 gctcagccat catgaaagag cctttcaatt ttgaaaataa agaagtggac taaaaagaat
    21481 aattaagcct agtccattct ttcacctaac cgttcaggtc agtgcggtga taagtgcatc
    21541 gtctggcccg accgtctccc cttgattttc caaaatatcc aaataatccg tgtaattggt
    21601 gacgatcaca ggtgtttcta aactgtatcc ttcctcttcg attttctttt tgtcgaagcg
    21661 gagcaacaga tcgccttgtt tcactctttc tccttgctgc accaagcttt caaaatattt
    21721 cccttccaac tgaacagtat ccaatccgac atgaatcagc aattccagac ctgcttcaga
    21781 aaccattccg attgcatgtt tcgtcgggaa cagggtcatg actgttccgt caaaaggggc
    21841 acgtacttct cctttttcag ggtagatcaa gattcctcga cctagtgttc cttgtgcaaa
    21901 agctgcatcc tttgctgcat tcagtggagc gatttgtcct tgtatcggtg tttttacaat
    21961 ttctttacct gatcttttct gtctatcatc tgtttcacct gcttcctcct tccaaaagaa
    22021 aaaagtcagc gcaaaggata ccaccatcgc aatggcgatt ccaatcaata cattaatcac
    22081 atccgttaaa tcaccatctg gtctaatata gttgggaatt gtaaagatgc ctaatccacc
    22141 tggattccaa gaagttaaat tcattaatcc actatacgcg ccccctaacg caccaccgac
    22201 acaggtaaaa ataaatattt ttttacgtgg caatgtaaac ccataaatag ctggctctgt
    22261 aatccccaca ataccagaaa caatggctgg tattgcgatt gctttgtctt ttgggtttct
    22321 aagcttaaag tagagtgcag taagtgctgc agtttgtcca aaagacacta ctgcaacagg
    22381 tgtcatgata ttttgccaac cattcacaga aaattgcatc aatcccattg gtacgattgc
    22441 ccaatggaga ccaaacatca ctagaatttg ccagaagaag cctacgattg ctccataaat
    22501 aactggcgaa agtgctaaaa gtgtttcaaa tccagccatt aaaatatcag ttgcgaatgt
    22561 gaatgctggt ccaacgagta aaaaacctaa gggcattgta ataagaacgg tgaagaatgg
    22621 aacgaagaaa ttcgcgatca tctcaggtac taacttttta attggtttct gaatttggct
    22681 agctaacaac acaataaaga taatgggaat gacagaagaa gcgtagttac gcgcaatcca
    22741 gggaatgccg aatactgttt gataaatagg tgcttcaaaa atagtgccag aaaatatggt
    22801 agtcagtggt gcttctgctg caccactcaa agcttccagc gacaagctag gattcattaa
    22861 tgctgcccca agcaacatcc ctacgaattc atttacttta aatttacggg cagctgttaa
    22921 accgataata acaggcataa attggaatac agaatcaccg atattatcaa acataacaaa
    22981 tgtgccagaa ccgcggtcaa atgcgcctaa gaagacaagt aatgctgtca gtccacgaat
    23041 catcccacca gcagctagaa tagcaaggaa tggctggaaa cagcccgata ttccatcaat
    23101 aagcatatca aacggtctca ttttttctgt tgaaacagcc tcatcaaaag taaggtttcc
    23161 tgcttttata acctcttcaa acacgactgg cacatgattc ccgatgacaa cttggtactg
    23221 tcctcccgct cgcattaccg tcacaacacc gtccatattt ttcaagatct catcatttgc
    23281 tttttcttcg ttttttaatt tgaaacgaag ccttgtgata caatgtgtca aaccattgat
    23341 attttccttc ccgccgacat tttctacaat tttgctcgct aattcttgat attttcccat
    23401 ttctattccc tccaatttat tagtggaagg tttagccaac tgaatgtcac tacccttatt
    23461 tcatgcgttc ttgttaattt atcctccttt ttttatttat ttacaacgat tctcatgcac
    23521 taaacgcgca atatggatcg ttaaatacat tttttcgtcc gtggacatct ggtagttgaa
    23581 tatctcggct aaaaagtctg ctattttctc aacgcaacca aacgtatcct tatatttttt
    23641 ttgaataacc tggtacaatt cctcagaaac ttctcccact tctgattttt ctccatctag
    23701 gacgcgtgtc agaaaaaaac gtaggtgtgt gacgaatcga aaaaaatgga ctgaatcttc
    23761 atcaaatgta atcttatagt aatagcggac gatatttaga atatcctgta ttgttgtagt
    23821 aaacaggtag gtattgtctt gttggctttg aatgccttgt gcattgacta aatgcaatgc
    23881 gataaatccg gcttcatctt ctgataactt gatgtggtat ttatccgaga ttttcttcac
    23941 tgtttttttc ccgatggcat actctttagg aaatagttgt ttgatatcat acagcaatac
    24001 atttggtaca aatattccca ttttgtgacg ttcgatagcc gtatacaaat gatccgtcaa
    24061 tgcaatatac accgaatcat tcaactggac ttgtagagcc tcttttgtat attcaatgat
    24121 ttcacaagaa atttctaagt actcaagagg aatcgcttgt gccaattctt ggaactgttg
    24181 tgaaagtccg tcatttgtca actgaaattt tttgtcgatg catgagtcaa aaatcgggtc
    24241 acccactttc tttttgaatg ccaaaccttt tcccatcacg atcatttcct gatttctttc
    24301 atccctggtt attacgacgt tgttattcag tactttctca atcagcatgt tttactcctt
    24361 tccatgatat atagggctgt atccaaaaca aaaagaaaaa cccaaaaagc cgtccatgga
    24421 accatgggag actcatcagg tttagcttgc atcaggcaat cactatccaa gttgtatcgt
    24481 tttctttata ttatcgaaag caagcacttt tgtcaacgct ttcttagcgg ctattgggca
    24541 cttctgacta gctgtcgtga ttttttttaa atctggtaat tacgacacta acgctcacag
    24601 atgaagaatc tgggaagctc tgttttattt tcactctagc gcttaggtca aaatgctact
    24661 atgctactgt cccaacttct aatatacggt gttccagtac cagcttatcg gtgataagcc
    24721 aaatttatgc aaaaatctgg aaagcctttt ttacaatttt tttcttatca cctaaagctg
    24781 aacggagccc tgtcacaacc actttttaca caccactgct ttgcagtttg tacatatctg
    24841 catacaaacc attttgcgct agaagtgttt catgattccc acgttctact atatgacctt
    24901 tgtctaaaac taaaatcaat tctgcatccc gaatcgtgga cagccgatgg gcaatcgcaa
    24961 tcgttgtccg tccttgtcgc atttttgtca atccttcttg gatcaatcct tctgtttcag
    25021 tatcgatatt tgctgtcgct tcatccaata ctagtatttt aggatctgtc acgattgttc
    25081 gcgcaaaagt gatcagctgt cgttgtccgc tagaatagct ggcaccgtgt tcgatcactt
    25141 ttgagtgata tccttttggt aattcatcga tgaacttgtt tgcttgaaca aactcagctg
    25201 catggcgaat ctgttcgtct gtgatagttg gattcatcat ccggatattt cctgcgatat
    25261 ctccataaaa caagaaggca tcttgcaaga ccaatcccat tttcttacgt aactcttcca
    25321 ttggatattc tcgaatgtca tgatcatcaa tcaagatctg gccttcataa aattcgtaaa
    25381 accgcatcaa tacgttgatg atcgagcttt ttccgcttcc ggtgtgcccc actaatgcaa
    25441 cggtctctcc cggattagcg acaaaagaga tattgttcaa tacattgtgc tctccatcat
    25501 aagaaaagct tacattgcga aactcgatct tacctgaagt aatcgcttga tctgctccag
    25561 gattttgttg cggtgcatat tcttctgtat ccatgatttt caaaatacga ctaccggcaa
    25621 ccattccatc tgtaaaggtg ctcaaaaagt ccatcatttg agacattgga ttaaaaaatc
    25681 cctggacata ggtcgtaaat gcatagatca gtccaacttc tacgggagaa tgcagcgcat
    25741 cgatcccaaa caaagtcaac gccaacgcaa tcgccaatgc atatagaaaa ttgatgatcg
    25801 gacttaacag aatcgaattt gttttgatca tcgcatagcg cgtgtctaaa tactcgttgt
    25861 tcacttgttc aaattcttta gctaaacgag cttcttggcg aaactgctgg ataatacgca
    25921 tgccggaaat cgattcattc aatttcgtgt tcagctgact taatttttcc cgcatattcc
    25981 ggtaaatacg agaactgaat ttctggtaat accagatgac gattcctaaa atcggcaaga
    26041 acaccaaagt ccacaaggca atttctccat tgatttgaaa catggcaatg aaggaagaaa
    26101 cgactgcaaa gatcccagtc aataccatca aaaagacata ccaaaattca aatagcgttt
    26161 ctgtgtcatt tgtcactcga gaaacagtcg atcctgccgg tgtctgatca aaataacgca
    26221 tacctagtgt atgcagcttt tcaaataatc ttacccgaac atactgatag gtcttcaatg
    26281 aagccatgga atacaaatac cattggaaaa accaaatgat acttttgact atgactccaa
    26341 acaagtacat accagcaaaa aacaaaataa cttgtgtcgt agccgactgt ttagccagat
    26401 gatcatccat aaaagtttgg atgatccttg gcaataggac attgatcaca gacagggcaa
    26461 acgcaaataa gatagcaacg atgaacgttc ttctaaaagg tttggcgaac ttcatcagtc
    26521 gtttgatgat cttcttctgt tctttaagtg gaatcgattt tgtccattcc gattgataat
    26581 tttcatccat caggcctcgc ctccttctat cttcgcttct aatttctgtt tctcccacat
    26641 tcgttcatac cagccgccta atgcaagcag ctcatgatgg gttcctcgtt caatgatttt
    26701 cccttgatcg acaacaatga tttcttttgc atgcatcacg ctgcttaaac ggtgagctgc
    26761 aatgatggtt gttttctctt ttcttatttc ttttaaatta tgcaagatag cttcttctgt
    26821 tttggcatct actgcagaaa gtgcatcatc taagatcagc aattcaggat ttttgatcaa
    26881 tgctcgagca atcgaaatcc tttgtttctg tccgccagat aaagagactc cacgttctcc
    26941 aactaaggta tcataacctt ttggtaattc cttgatttca ttgttgataa atgctaaaga
    27001 agccgcatgc tctacttctt cttctgaaag ttttggatta gaaaaacgga tattatctcg
    27061 aatggtcatc gaaaacaaga agtgatcttg aggcacatag ccgattgatc ctaataaagc
    27121 attcaatgaa tattctttga tcgaacatcc gccaaattga atttttcctt gataatcatc
    27181 gtattcacgc atcagtaatt tgatgattgt cgtttttccc gcaccagttt tcccaacaat
    27241 acctaatgtt tctccttctt ggatcgcaaa tttgatttgt tctaatgctg gtgattcttc
    27301 tttcggatag ttaaaagagg aaatagctac attcagctcg ccttttgccg gagtctgcac
    27361 tgcacccagt tgttccacga tatgcgtctt ttcatgcaaa agttcattga cgcgatcata
    27421 agaagcattt cctctttcca atacgttgaa caatcgacca atcgcaaaca tcggccaaac
    27481 aagcataccg atatagctaa taaatgagac catctgcccg attgagatcg tgccttccaa
    27541 gacatacttt ccacctaaaa taattgttgc aacataagac agaccgataa tcaaggtaat
    27601 gaatggatca aataaggagt ctaaaaaatt gactcgtttg tttttaataa ttgcatcttc
    27661 aatcttttct tcaaaatccg cgagatcttc tttttcttgt ccaaatgttt taatgacttt
    27721 gatcccagta atactttctt gtgtcttatc attgatacta gaaaaagctt cttgcgaatc
    27781 acgaaaagca tcgtggagtt ttgaccccag cactcgtgaa gtaacggcta gtaaaggcag
    27841 aggaatcagc gcaatcagtg ttaagcgcca atcaataaac aaaatcatcg caatgatcgt
    27901 tgttccgccc gtgatcactg aatctgcaaa ggtcaagata ccagctcctg ctacattttg
    27961 gattgcattc aaatcattcg ttgcatgagc catcaaatct ccagtccgat atttttggta
    28021 aaagagattg tccatttctg taaaatgttt gaatagctgc tgccgaagtt ctttctccaa
    28081 ccgagctgca cttccccaaa tattcgtccg ccagatataa cgaaaaagat attgtaagag
    28141 agctgcaagg atcaataata agatccacca ggaaatgatc tttattgata aatttttatc
    28201 ggcgatctca tcaatgatga tcccaatcac ttttggagga gctaactgta ccaaggccac
    28261 tgcgaatagg gagaaaatcc caattaaata atgtttcttt tcctgtcgaa aaaaccaacc
    28321 taattttcta aaaatcgaca ttttactcct cctttgtctt tatcaagagc ataggattgt
    28381 ctgtgcttgt cttttttgct gaatagtctg aatagtctaa caaattacga aataaaaaac
    28441 ggactgaaaa aatcagcccg caaaaagtct ttgggtcaga acctattttt tcccttggtt
    28501 cttcatttgc tgcatcatct ggcgaatttt cttctcagat ggtttttgcc ccattgacat
    28561 catcattgat cgcaacatgt cctcattaac gggaggattt ttttctaaat agtctttcat
    28621 atatttacgc gctaggaaaa agccacctac tgcgccgatt agcaaagcaa taatagcaat
    28681 aagtacgaca atacctgttg ataccatata aatctctcct ttctatttcc gaattgctct
    28741 caatgattta ttttactaaa gtttattgaa aaaagaaagg cgtattcttt agtttcatgc
    28801 aaaaaatagt ggttttttta gagaagaaca aaatttcttt cattaaccag caagacacgc
    28861 cacgaaaaaa aattaggcat accaaatctt tttctttaat tttttctttc tgaggtgtag
    28921 aatgtaatcg tagataaata ggaaaggtgg ttttttcttg aataatcaat ccaacaaaca
    28981 atttcataag catcgactta ttgaacatac aaatggttta tctttagctg aagtaaatgg
    29041 aacagtggat gtacccaagg gaaaaagttt ttttaaaaca cttcttgctt actctggacc
    29101 aggtgcatta gtagcagttg gttatatgga tccaggtaac tggtccactt cgatcactgg
    29161 tggacaaaat ttccaatatt tattgatgtc agttattttg atgtctagtc tgattgctat
    29221 gcttttacaa tatatggcag caaaattagg aatcgtgacc caaatggatt tggcccaagc
    29281 aatccgagca cggacaagta agacattggg tgtcatttta tggatcttga ctgaattagc
    29341 cattatggca acagacatcg cagaagtcat tggtgcagca atcgccctct acctgctttt
    29401 tgatattcca ttggtaattg ccgtatttat cactgtgtta gatgttttcg tccttttatt
    29461 gctgacaaaa gtcgggtttc gtaaaatcga agctttggtt gtctgcttga ttttcgtcat
    29521 tttattcgtt ttcgtctatc aagttggctt gtctaaccct gattggcaca gtttgtttgc
    29581 cggatttatc cctaacagtc acacattctc agactcaccg gtcatcggcg gcgaaactcc
    29641 gctgactgga gctttaggga ttattggtgc aacagtcatg ccgcataact tatatcttca
    29701 ttcagcagtt tctcaaactc ggaagatcga tcaccaagac gaagaagatg tagcacaagc
    29761 ggtacgcttt tctacttggg attctaatat ccagttgtca atggcttttc tagtcaatgc
    29821 actgctattg atcatgggtg tggctgtatt caaaaccggt tctgtccaag atccttcctt
    29881 ttttggtctg tatgaagctt tatctgatcc atcagctatg agcaatggta tcttgattgc
    29941 tgtagcaaaa tctggtatct tgtctacttt atttgctgta gcacttcttg cttctggcca
    30001 gaactctact attactggta cgttgaccgg tcaagtcatc atggaaggct tcatccatat
    30061 gcgtatccca atttggctaa gaagactagt cacacgattg ctttctgtga ttcctgtatt
    30121 gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa gaacataccg ctttgaataa
    30181 cttgatgaat gaatcccaag tttttctggc ttttgcattg ccattctcga tgattccttt
    30241 actgatgatg accaatagcc aagcggagat ggggagccga ttcaaaaatt catttgttgt
    30301 aaaactactt ggctggttct ctgtgattag tctgacttac ttgaatttac gaggattacc
    30361 aggtcaaatc gaaagtttct tcggcgagca agccacctca gctcaactag cattggctga
    30421 tcaaatcgct tatgtaatca ttgttcttgt ccttctcctg ttggcatgga cgattggtga
    30481 tttatataaa ggaaacaaaa aatatgaaca gcgtttgcag gaaacgatag aagaaatcga
    30541 gtagaaactg attcttttac acaaaggagg ttgtgtcaga agtgggcagc ttcaagaaat
    30601 aagaaggaat ttaagaaaat tgcttttcaa atttttgtga aatttcggct tatttccgaa
    30661 gaagttactt ctggaacacc gtgtatcaga ggttgtgaaa aagctgcctt gcatcaagta
    30721 ataagaacag ccaagtaaaa acagcttttt atgtttttaa cttggttgga cttattaccg
    30781 cagatgcaag cttttgagca ccgtttattc ggaaaaagga actgcgacaa gacttttgtc
    30841 acagttccct ttcctacgat tagtcaagta aaaggtgtaa gaaaaatagc tcataaacga
    30901 aagcgacaaa atcaa
//
LOCUS       DAYYAO010000029        30516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000029 DAYYAO010000000
VERSION     DAYYAO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.030"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACHARI_001200"
     CDS             complement(<1..208)
                     /locus_tag="ACHARI_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442257.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIIS"
     gene            complement(196..825)
                     /gene="pyrE"
                     /locus_tag="ACHARI_001201"
     CDS             complement(196..825)
                     /gene="pyrE"
                     /locus_tag="ACHARI_001201"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8442258.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(832..1548)
                     /gene="pyrF"
                     /locus_tag="ACHARI_001202"
     CDS             complement(832..1548)
                     /gene="pyrF"
                     /locus_tag="ACHARI_001202"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8442259.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1545..2477)
                     /locus_tag="ACHARI_001203"
     CDS             complement(1545..2477)
                     /locus_tag="ACHARI_001203"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8442260.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2474..3250)
                     /locus_tag="ACHARI_001204"
     CDS             complement(2474..3250)
                     /locus_tag="ACHARI_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8442261.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3304..6489)
                     /gene="carB"
                     /locus_tag="ACHARI_001205"
     CDS             complement(3304..6489)
                     /gene="carB"
                     /locus_tag="ACHARI_001205"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8442262.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISNKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6494..7570)
                     /locus_tag="ACHARI_001206"
     CDS             complement(6494..7570)
                     /locus_tag="ACHARI_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8442263.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7575..8855)
                     /locus_tag="ACHARI_001207"
     CDS             complement(7575..8855)
                     /locus_tag="ACHARI_001207"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8442264.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8874..9800)
                     /locus_tag="ACHARI_001208"
     CDS             complement(8874..9800)
                     /locus_tag="ACHARI_001208"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8442265.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9813..11096)
                     /locus_tag="ACHARI_001209"
     CDS             complement(9813..11096)
                     /locus_tag="ACHARI_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8442266.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11110..11649)
                     /gene="pyrR"
                     /locus_tag="ACHARI_001210"
     CDS             complement(11110..11649)
                     /gene="pyrR"
                     /locus_tag="ACHARI_001210"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8442267.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11884..12795)
                     /locus_tag="ACHARI_001211"
     CDS             complement(11884..12795)
                     /locus_tag="ACHARI_001211"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8442268.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12792..13256)
                     /gene="lspA"
                     /locus_tag="ACHARI_001212"
     CDS             complement(12792..13256)
                     /gene="lspA"
                     /locus_tag="ACHARI_001212"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8442269.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13261..13959)
                     /locus_tag="ACHARI_001213"
     CDS             complement(13261..13959)
                     /locus_tag="ACHARI_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8442270.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13946..14332)
                     /locus_tag="ACHARI_001214"
     CDS             complement(13946..14332)
                     /locus_tag="ACHARI_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442271.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14479..16149)
                     /locus_tag="ACHARI_001215"
     CDS             complement(14479..16149)
                     /locus_tag="ACHARI_001215"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8442272.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16332..16565
                     /locus_tag="ACHARI_001216"
     CDS             16332..16565
                     /locus_tag="ACHARI_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8442273.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16664..17095)
                     /locus_tag="ACHARI_001217"
     CDS             complement(16664..17095)
                     /locus_tag="ACHARI_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8442274.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17174..17587
                     /locus_tag="ACHARI_001218"
     CDS             17174..17587
                     /locus_tag="ACHARI_001218"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8442275.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17625..18773)
                     /locus_tag="ACHARI_001219"
     CDS             complement(17625..18773)
                     /locus_tag="ACHARI_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8442276.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18895..19491)
                     /locus_tag="ACHARI_001220"
     CDS             complement(18895..19491)
                     /locus_tag="ACHARI_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8442277.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19691..22333)
                     /gene="alaS"
                     /locus_tag="ACHARI_001221"
     CDS             complement(19691..22333)
                     /gene="alaS"
                     /locus_tag="ACHARI_001221"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8442278.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22721..23152
                     /locus_tag="ACHARI_001222"
     CDS             22721..23152
                     /locus_tag="ACHARI_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8442279.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23197..24546)
                     /locus_tag="ACHARI_001223"
     CDS             complement(23197..24546)
                     /locus_tag="ACHARI_001223"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8442280.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24616..25779)
                     /locus_tag="ACHARI_001224"
     CDS             complement(24616..25779)
                     /locus_tag="ACHARI_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8442281.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25908..26231
                     /locus_tag="ACHARI_001225"
     CDS             25908..26231
                     /locus_tag="ACHARI_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442282.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26414..27763)
                     /locus_tag="ACHARI_001226"
     CDS             complement(26414..27763)
                     /locus_tag="ACHARI_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8442283.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27832..28785)
                     /locus_tag="ACHARI_001227"
     CDS             complement(27832..28785)
                     /locus_tag="ACHARI_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8442284.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28887..30077)
                     /locus_tag="ACHARI_001228"
     CDS             complement(28887..30077)
                     /locus_tag="ACHARI_001228"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8442285.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(30277..>30516)
                     /locus_tag="ACHARI_001229"
     CDS             complement(30277..>30516)
                     /locus_tag="ACHARI_001229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442286.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         8576 a         6691 c         5353 g         9896 t
ORIGIN      
        1 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
       61 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
      121 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
      181 cttcatcaag cgcgcttaaa tctgccaatt ttcaggatct tttttccatt cttttagcag
      241 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
      301 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
      361 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
      421 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
      481 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
      541 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
      601 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
      661 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
      721 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
      781 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
      841 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
      901 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
      961 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     1021 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     1081 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     1141 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     1201 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     1261 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     1321 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     1381 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     1441 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     1501 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     1561 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     1621 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     1681 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     1741 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     1801 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     1861 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     1921 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     1981 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     2041 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     2101 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     2161 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     2221 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     2281 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     2341 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     2401 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     2461 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     2521 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     2581 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     2641 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     2701 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     2761 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     2821 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     2881 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     2941 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     3001 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     3061 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     3121 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     3181 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     3241 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     3301 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     3361 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     3421 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     3481 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     3541 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     3601 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     3661 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     3721 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     3781 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     3841 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     3901 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     3961 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     4021 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     4081 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     4141 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     4201 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     4261 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     4321 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     4381 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     4441 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     4501 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     4561 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     4621 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tattcgaaat
     4681 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     4741 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     4801 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     4861 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     4921 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     4981 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     5041 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     5101 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     5161 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     5221 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     5281 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     5341 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     5401 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     5461 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     5521 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
     5581 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
     5641 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
     5701 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
     5761 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
     5821 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
     5881 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
     5941 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
     6001 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
     6061 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
     6121 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
     6181 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
     6241 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
     6301 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
     6361 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
     6421 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
     6481 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
     6541 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
     6601 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
     6661 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
     6721 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
     6781 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
     6841 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
     6901 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
     6961 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
     7021 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
     7081 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
     7141 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
     7201 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
     7261 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
     7321 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
     7381 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
     7441 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
     7501 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
     7561 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
     7621 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
     7681 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
     7741 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
     7801 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
     7861 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
     7921 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
     7981 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
     8041 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
     8101 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
     8161 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
     8221 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
     8281 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
     8341 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
     8401 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
     8461 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
     8521 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
     8581 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
     8641 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
     8701 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
     8761 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
     8821 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
     8881 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
     8941 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
     9001 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
     9061 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
     9121 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
     9181 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
     9241 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
     9301 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
     9361 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
     9421 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
     9481 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
     9541 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
     9601 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
     9661 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
     9721 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
     9781 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
     9841 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
     9901 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
     9961 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    10021 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    10081 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    10141 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    10201 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    10261 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    10321 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    10381 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    10441 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    10501 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    10561 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    10621 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    10681 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    10741 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    10801 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    10861 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    10921 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    10981 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    11041 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    11101 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    11161 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    11221 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    11281 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    11341 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    11401 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    11461 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    11521 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    11581 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    11641 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    11701 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    11761 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    11821 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    11881 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    11941 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    12001 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    12061 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    12121 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    12181 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    12241 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    12301 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    12361 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    12421 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    12481 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    12541 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    12601 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    12661 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    12721 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    12781 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    12841 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    12901 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    12961 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    13021 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    13081 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    13141 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    13201 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    13261 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    13321 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    13381 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    13441 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    13501 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    13561 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    13621 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    13681 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    13741 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    13801 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    13861 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    13921 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    13981 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    14041 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    14101 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    14161 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    14221 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    14281 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    14341 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    14401 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    14461 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    14521 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    14581 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    14641 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    14701 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    14761 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    14821 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    14881 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    14941 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    15001 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    15061 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    15121 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    15181 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    15241 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    15301 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    15361 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    15421 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    15481 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    15541 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    15601 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    15661 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    15721 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    15781 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    15841 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    15901 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    15961 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    16021 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    16081 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    16141 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    16201 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    16261 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    16321 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    16381 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    16441 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    16501 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    16561 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    16621 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    16681 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    16741 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    16801 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    16861 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    16921 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    16981 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    17041 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    17101 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    17161 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    17221 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    17281 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    17341 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    17401 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    17461 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    17521 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    17581 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    17641 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    17701 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    17761 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    17821 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    17881 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    17941 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    18001 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    18061 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    18121 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    18181 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    18241 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    18301 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    18361 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    18421 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    18481 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    18541 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    18601 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    18661 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    18721 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    18781 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    18841 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    18901 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    18961 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    19021 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    19081 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    19141 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    19201 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    19261 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    19321 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    19381 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    19441 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    19501 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    19561 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    19621 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    19681 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    19741 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    19801 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    19861 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    19921 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    19981 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    20041 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    20101 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    20161 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    20221 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    20281 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    20341 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    20401 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    20461 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    20521 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    20581 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    20641 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    20701 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    20761 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    20821 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    20881 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    20941 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    21001 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    21061 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    21121 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    21181 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    21241 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    21301 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    21361 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    21421 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    21481 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    21541 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    21601 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    21661 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    21721 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    21781 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    21841 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    21901 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    21961 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    22021 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    22081 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    22141 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    22201 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    22261 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    22321 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    22381 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    22441 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    22501 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    22561 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    22621 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    22681 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    22741 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    22801 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    22861 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    22921 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    22981 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    23041 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    23101 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    23161 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    23221 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    23281 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    23341 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    23401 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    23461 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    23521 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    23581 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    23641 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    23701 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    23761 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    23821 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    23881 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    23941 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    24001 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    24061 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    24121 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    24181 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    24241 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    24301 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    24361 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    24421 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    24481 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    24541 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    24601 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    24661 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    24721 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    24781 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    24841 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    24901 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    24961 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    25021 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    25081 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    25141 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    25201 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    25261 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    25321 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    25381 ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat taaaagctgg
    25441 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    25501 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    25561 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    25621 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    25681 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    25741 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    25801 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    25861 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    25921 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    25981 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    26041 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    26101 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    26161 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    26221 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    26281 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    26341 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    26401 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    26461 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    26521 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    26581 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    26641 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    26701 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    26761 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    26821 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    26881 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    26941 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    27001 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    27061 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    27121 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    27181 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    27241 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    27301 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    27361 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    27421 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    27481 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    27541 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    27601 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    27661 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    27721 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    27781 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    27841 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    27901 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    27961 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    28021 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    28081 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    28141 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    28201 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    28261 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    28321 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    28381 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    28441 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    28501 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    28561 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    28621 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    28681 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    28741 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    28801 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    28861 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    28921 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    28981 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    29041 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    29101 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    29161 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    29221 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    29281 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    29341 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    29401 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    29461 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    29521 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    29581 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    29641 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    29701 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    29761 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    29821 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    29881 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    29941 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    30001 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    30061 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    30121 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    30181 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    30241 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    30301 aaatacaaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    30361 taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc gtttatcagc
    30421 aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa ttcgaaaaaa
    30481 ggactgtgac aaaagtcttg tcacagtcct attctt
//
LOCUS       DAYYAO010000030        29959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000030 DAYYAO010000000
VERSION     DAYYAO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.031"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..614
                     /locus_tag="ACHARI_001230"
     CDS             <1..614
                     /locus_tag="ACHARI_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8442287.1"
                     /translation="VFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVGK
                     YMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGTG
                     VFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPFI
                     LALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            611..1384
                     /locus_tag="ACHARI_001231"
     CDS             611..1384
                     /locus_tag="ACHARI_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8442288.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1381..1779
                     /locus_tag="ACHARI_001232"
     CDS             1381..1779
                     /locus_tag="ACHARI_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8442289.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1964..2635
                     /locus_tag="ACHARI_001233"
     CDS             1964..2635
                     /locus_tag="ACHARI_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442290.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2694..3134
                     /locus_tag="ACHARI_001234"
     CDS             2694..3134
                     /locus_tag="ACHARI_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442291.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3146..3814
                     /locus_tag="ACHARI_001235"
     CDS             3146..3814
                     /locus_tag="ACHARI_001235"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8442292.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3878..4501
                     /locus_tag="ACHARI_001236"
     CDS             3878..4501
                     /locus_tag="ACHARI_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8442293.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4557..4943
                     /locus_tag="ACHARI_001237"
     CDS             4557..4943
                     /locus_tag="ACHARI_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8442294.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5156..6547
                     /locus_tag="ACHARI_001238"
     CDS             5156..6547
                     /locus_tag="ACHARI_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8442295.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7158..7526
                     /locus_tag="ACHARI_001239"
     CDS             7158..7526
                     /locus_tag="ACHARI_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8442296.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7706..8647
                     /locus_tag="ACHARI_001240"
     CDS             7706..8647
                     /locus_tag="ACHARI_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8442297.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8716..9447
                     /locus_tag="ACHARI_001241"
     CDS             8716..9447
                     /locus_tag="ACHARI_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8442298.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9591..11771
                     /locus_tag="ACHARI_001242"
     CDS             9591..11771
                     /locus_tag="ACHARI_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8442299.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11791..12273
                     /locus_tag="ACHARI_001243"
     CDS             11791..12273
                     /locus_tag="ACHARI_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8442300.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12289..13050
                     /locus_tag="ACHARI_001244"
     CDS             12289..13050
                     /locus_tag="ACHARI_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8442301.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13181..14515
                     /gene="murC"
                     /locus_tag="ACHARI_001245"
     CDS             13181..14515
                     /gene="murC"
                     /locus_tag="ACHARI_001245"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8442302.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14822..15307
                     /locus_tag="ACHARI_001246"
     CDS             14822..15307
                     /locus_tag="ACHARI_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8442303.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15300..15983
                     /locus_tag="ACHARI_001247"
     CDS             15300..15983
                     /locus_tag="ACHARI_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8442304.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16109..18754
                     /gene="polA"
                     /locus_tag="ACHARI_001248"
     CDS             16109..18754
                     /gene="polA"
                     /locus_tag="ACHARI_001248"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8442305.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHANTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18799..19635
                     /gene="mutM"
                     /locus_tag="ACHARI_001249"
     CDS             18799..19635
                     /gene="mutM"
                     /locus_tag="ACHARI_001249"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8442306.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19599..20228
                     /gene="coaE"
                     /locus_tag="ACHARI_001250"
     CDS             19599..20228
                     /gene="coaE"
                     /locus_tag="ACHARI_001250"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8442307.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            20280..20612
                     /locus_tag="ACHARI_001251"
     CDS             20280..20612
                     /locus_tag="ACHARI_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8442308.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20924..21415
                     /gene="nrdR"
                     /locus_tag="ACHARI_001252"
     CDS             20924..21415
                     /gene="nrdR"
                     /locus_tag="ACHARI_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8442309.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21438..22811
                     /locus_tag="ACHARI_001253"
     CDS             21438..22811
                     /locus_tag="ACHARI_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8442310.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22811..23737
                     /gene="dnaI"
                     /locus_tag="ACHARI_001254"
     CDS             22811..23737
                     /gene="dnaI"
                     /locus_tag="ACHARI_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8442311.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23866..24306
                     /locus_tag="ACHARI_001255"
     CDS             23866..24306
                     /locus_tag="ACHARI_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8442312.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            24450..25151
                     /locus_tag="ACHARI_001256"
     CDS             24450..25151
                     /locus_tag="ACHARI_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8442313.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQNPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            25148..26269
                     /locus_tag="ACHARI_001257"
     CDS             25148..26269
                     /locus_tag="ACHARI_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8442314.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            26284..27516
                     /gene="pepT"
                     /locus_tag="ACHARI_001258"
     CDS             26284..27516
                     /gene="pepT"
                     /locus_tag="ACHARI_001258"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8442315.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            27648..28556
                     /locus_tag="ACHARI_001259"
     CDS             27648..28556
                     /locus_tag="ACHARI_001259"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8442316.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            28591..28863
                     /locus_tag="ACHARI_001260"
     CDS             28591..28863
                     /locus_tag="ACHARI_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8442317.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            28874..29920
                     /locus_tag="ACHARI_001261"
     CDS             28874..29920
                     /locus_tag="ACHARI_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8442318.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9970 a         5143 c         6583 g         8263 t
ORIGIN      
        1 ttgtttttgc accgttagaa ggagaagacc tcgcttcatt tctggttggt gcggcaatca
       61 tgatgatcgg aatgacgctc tttttgtttg gagcagatta ttctatgatg gaagtcggag
      121 atctagtagg gaaatatatg atcaaaaaga aaagtttgac cgttttggtc agtttgggat
      181 ttgctatcgg gatcgtgatc acgattgctg aaccttccgt gcaagttttg ggacaacaag
      241 tatatgatat atctgacgga gagattggca gagtgttgct gattgggatc gtcagcgtcg
      301 gcactggagt atttctagcc tttgcgctgc ttcgggtcgt attcaagcta tcctattatc
      361 aattgatggc tataggctac ataggcgttt tgatcgcctc atttttcacc agttccgaat
      421 ttatgccgat tgcttttgat tcggggggag tgactactgg tccagtaact gttccattta
      481 tcttggccct tgccggaggg atgactagta tgatcaaaca aaagaaaaat gaaaatgaca
      541 gttttggaat ggtcggtatt gcttcgttag ggccgattct tgccgtgatg attttggggg
      601 tgatcttcca atgagtttct ggcagcaagt gatagatgga atcgaaatcg ttaccatgga
      661 agtcattttg gcagtggcac ctatcgtaat cctatttgtt ttaatgaata tcattagttt
      721 tcgtatgcgc aaaaagcgtt tctttgacat tatgaaaggt ttcttgataa cgactattgg
      781 gttgatcctt ttcctgcacg gagtcaatat ggcttatgta ccggtaggac agcatttagg
      841 cagtgctatt gcgggattgc cttacaatta tatactggtt ccccttggct ttttaatggg
      901 gttcttagta gggtttgctg agccggcgat tcatgtaatg atcaaacagg ttgaagaatt
      961 gagcgagggg agaatccgat cgaaaatcat gctgatggta atttcaattg ggatcggcgc
     1021 agcagtagga ttgtctatgt ggcgtttgct cgtcggattc tctctttatt atttcttgat
     1081 cccaggatac gggctagcat ttattttagg acgaaaagtg gacaagatct ttttagcaat
     1141 ggcttttgat aacggtggag tagcaacggg cccgatgtgt tcgacattta ttttatcaat
     1201 gagcgtggca atcgctgcac aaattgaagg aagagatccg atcattgacg ggtttggtgt
     1261 ggttgcaatg attgcattga caccaatatt atccacttta gtactaggat atatttataa
     1321 acgaaaagat gaaaaagaca gaatcgaaaa agaaagattg caaaaattgg gggaaagcaa
     1381 atgaaagatc ttgtagcatt gaatcttcac atgattgtga cgatcgttga cggaggaatg
     1441 ggcgaagatg ttatcgactt ttcaaaagaa gcaggtgcag caggcggaac gatcttacat
     1501 ggaagaggtt ctggtgtaca tgattcaggg aaatttttag gtttggacat ttcgccagaa
     1561 aaagatattg tgttgacatt agtacccgat agtttgacga ataaagtaat ggatgcgatt
     1621 ggtcaaggca tcaacatcga tattccaggt aatggtattt gttttagtat tgatgtagac
     1681 aaagtcattg gtatcactaa gattcatcaa tatcgagaaa ttatcggaat gcaagaactt
     1741 ttagatgaaa ataacgatag agaaaagtct gaatcataga caagcgtttg aagaggaaat
     1801 aaacacaaac aagaaggcgg atgagcgtta tttccgcttt tttgtttgta tttttctcgt
     1861 gaatacttat gaaatagtaa atttttatga aattctactc taagcatcag actttctttt
     1921 caaaatatgc tattttaaaa atgatgaaaa aagggggaga aaaatgaatt ttttagttgt
     1981 tttaaaagat actctgattg agcgatttac atttctatat gttcaattgc aggaaaataa
     2041 aacattgctg catttttcac cagagttcca tctagagggg ctaacattag gagaaatcta
     2101 tcaagcagaa gggctgtcgg ctgttttgca cttttttaag gtagaattgg aattaccagt
     2161 aaaagattat atcgagcttt ctcttgaatc ttgggatcga gcagtggttg aactgtttcc
     2221 agaaggttta atgatcgata caaacgatgg gaagatccat ctgactgcag cagaactgca
     2281 aaaacaaatg cgttatcaaa tagatgacga aaattctttt tctattttta gacgtcagca
     2341 aaagattcta aaaagctttt taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac
     2401 acagctgttg aaaaaatatc caggtctgct tcacacgtct attcaatttc ctcaattgat
     2461 tacgatgatc catcgtttta tacgtatgca acagctacca tctaagaagc tgtttcttcc
     2521 attgacggac acatttcgag tagtcaacag agaagataaa aagaaagtga ttgtgattga
     2581 ctttaccaga aatcggaatg tccttcacca agttgcaatg gaaaaggcca attaaggctt
     2641 tttctttttg gatttttttg tcataataaa tattagatga tggaggagat aatatgatgc
     2701 aaacatggtt agaaaggcta atacgatttc agcagaacta tcacaaactt atgcagggaa
     2761 gatatgcgaa atttgatcag ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt
     2821 tgctaagtcg ttggttgccc tatagaatcg gacaattgtt gttcatcgtc tttttcggat
     2881 ggatgatatt tcgtttccta tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt
     2941 atttgaaata tcttgataga gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag
     3001 tcaaaatgga tcgaaaaagt tatacttttt ttgagtgccc gaattgtcat caaaagcagc
     3061 gtgcacctaa aggaaaagga cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg
     3121 aaacgaatgt ttaagggaga aaagaatgaa attagtatta ttacgtcatg gcgaaagcga
     3181 agcaaatttt gaaaattact ggacaggatg gctagatgtc gccttaacag aaaaaggaaa
     3241 agaacaagca gcagttgccg gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt
     3301 ttatacctct gttttgaaac gtgcgattct taccggacaa gtcgcactag aagaaatggg
     3361 tcagttatgg cttcctataa taaaaacatg gcgtctaaat gaacggcatt atggggatct
     3421 tgtgggtaaa aacaaagata agatgaaaga aatatacgga aaagatcaag taaaaacatg
     3481 gcgcagagga tatacggaag ttccgccgtt aaccgcagaa aatagatttg accgcagata
     3541 tgatcacttg gatccgcatt tgatcccata cggagaaagt ctggaaatga cagtgaatcg
     3601 gatcattccc ttatggcaag atcatctagc accaaaacta aaagatcggc aagatttact
     3661 agttgtgggt catggtaata gcatccgagc actgacaaaa tatttagaag atgttcctga
     3721 agatcaaatg gatacgatcg atatcccaaa tgcacaacca atccagtata cattagcaga
     3781 tgatttgaca attctggaca aaaaaatact ttaaagaaca cctttatcta aaagtgagag
     3841 atagaaaaaa gaagcaaagg aatggaacaa aaggaacatg aaaaattata tatgggactt
     3901 tgatggtaca ttgtttgata cgtatcccgc aatggtagat ggtgcatggc aagcactaaa
     3961 agattttggt atcagtatgg acaaaaaaga aatctacttc aaaatgaaaa agtattctac
     4021 tagttatttg atcaatgaat caaacttgaa tgccagagag tttaatgaat tatttcaccg
     4081 atatgaaaaa gaaagtacgg aagtttcccg tccatttcca gaaacgaaac aagtactgga
     4141 aatgctaaaa gacaacggcg gtcgtcattt catcctgacc caccgtttga cagaatcaac
     4201 atggggcctg ttaaaagaac atcgattggc acatctgatt gaggaagtag tagggatcga
     4261 tcaggacttt cctcgtaaac ctgatccagc atctttaaat tatttgatcg atacttttca
     4321 tttagaaaga acagacacga tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa
     4381 aaatgcagga gtcgctactt gtctttacga catcgatcat tttctgggtg agatcccggc
     4441 agattatgtg gtaggaaatc tgaatgaaat ccttaccttg cagagcatca ttaaaaagta
     4501 gtatcccctt tcaaaggaga tatgttataa ttttcataaa ggagtgataa aattatatgg
     4561 aaatcagaag acaaaaagaa gttgtagaag catttcatta tgatatgaga ttgccgaacc
     4621 aagaagtaga aacagatttg aaagtaggct tctcgccttt aaaagaaacg atcgaaaact
     4681 atccgaaaga acatagcatc gtaggagcac gcttagagtt ccgtttagtg tttgatacat
     4741 atgttttatc tggaagtgtg agtcagatca atcatatcct caatcggaaa attgaaggtc
     4801 aaaatgatgt cacacaagaa gaagtggacg agctagtagc accgttattc gatatcgtac
     4861 gccgaatgtc ttatgaggtg acagagatcg ccttagatca gcctggggta aaattgaatt
     4921 ttcaatcaga aaaacaagaa tagacagtaa aaacatacct gcgatagatt aaagctatca
     4981 caggtatgtt tttttattac taattgtaag caaaaagaat gtcatttcga taaaaatcgt
     5041 ctaaaatata aaagtcactg aaaaaaattt tttcaggagg atggttgaag aatcattctc
     5101 gggaaccttt acttccttcg atggacttgt cattaataat atgaggagga gaaaaatgac
     5161 aacactcatc acagttttag tggttctagt ttttgcggca ttattatatg tgttctatcg
     5221 tatgcaaaag cgccatacaa aattttcaac acgcgtcttt acagcattag gagttggtat
     5281 cgtctttggc ggtctgcttc aattgatctt tggtgtagac gacaaagtcg ttactcaatc
     5341 aatggaatgg atcgggattg taggtcaagg atatgtatca ttcttgcaaa tgctagttat
     5401 gcctttagtg tttatttcga tcattggtgc attcacaaaa atgaaagttt ctgaaaagct
     5461 agggaaaatt agtgcgactg tattgacttc tttacttgga acaacagcta ttgcagcatt
     5521 gatcggaatt ggttcaacaa tgctgttcgg actccaagga gctaaattca cacaaggagc
     5581 tgcagaaaca gcacgaatct ctgaactggc aacacgtcag gaaacagttg aaaacttatc
     5641 gattccacag cagattttat cctttatccc taaaaacgtt tttgctgatt tagcaggtag
     5701 tcgtgcaaca agtacgattg gagtcgttat tttcgcggca tttgtcggaa tcgcttatct
     5761 aggcgtgaaa agaaaagcgc caaaagaagc agcattcttt tcagatttag tcgacagtct
     5821 gtataaaatc attatgcgga tcgtgacgct tgttttgcgt ttgacacctt atggagtatt
     5881 tgctttgatg acaaacgttt tggcaacaag tgattttgca gctatcgtca atttaggtaa
     5941 atttgtcgtt gcttcttacg tagcattgct tgctgttttg attgtccact cactgatttt
     6001 gattggtgca aaagtcaatc caatcaacta tttcaaaaaa gcctttccgg tattgagctt
     6061 tgcgtttact tcacgttcaa gtgctggagc tttgccattg aatatcgaga ctcagacaaa
     6121 agctttagga gtagatgatg caacagctaa tttctcagca agttttggtt tgtctattgg
     6181 acaaaacggc tgtgctggcg tttatccagc tatgttggca acgatcgtcg caccagcagc
     6241 tggagtgaat gtctttagtt tggaatttat tttgatgctt gtggcaattg taacaatcag
     6301 ttcttttggt gtagctggag tcggtggggg cgcaacattt gcttcactga tcgttttagg
     6361 ggcaatgaac ttgccagtag caatcgttgg gttagttatc tctgttgaac cattaatcga
     6421 tatggcacgt acagcagtaa acgtcagcgg tagcatggta gctggtatcg tgacaagtgc
     6481 acgcatcaaa gatttagatc gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa
     6541 tgcatagtca aaaagaggtt gtgatagaag tggtcagctt taagaaataa gaagaagttt
     6601 cctaaaaata gtttctcaaa tttttgtgaa atttcggctt atttccgaga agctacttct
     6661 gaaacaccgt gaataagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
     6721 aaacaaaaat agttcctcat attttacgtt tggttgagct tattaccgca gatgcaagct
     6781 tttgaacacc gtttaagaaa gaggtcgtga gcctgccgtt tcactctgag tcacaaagag
     6841 caatttcaaa aaatagcttc tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg
     6901 tcaggagaac accgtttacc agaggttgtg acttgttttt agtcacgacc tcttttttta
     6961 tttattccat ataaatacac attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac
     7021 aaccaactta tttattatta aagaaaagcg tatttaagta ggacgattct gttgagagct
     7081 ttcgtagatt ttcccatttt cctatggtaa actgacaaag ttgagaatgt tagaacagat
     7141 cgtttaggag gaaacagatg tttacgaatg aaataaaaat catgctttat gtggacgatg
     7201 tagaaaaaaa tgcaacgttt tggagagcag ttggctttgc ggaaaaagat cgtcaagaaa
     7261 tggatgggac gcttataatt gaagtaggag ttgaggacag tcaaacctca tttctgttat
     7321 atgatcgagc atttgttgag tcccattcac ctggcacagc tactagtgcc ccatctttga
     7381 tgttttacag taaagatatc tttggactat ataaaaaaat gcagacgcaa tcagtcacat
     7441 taggtgatat ggtacagctt ggagaagaat atgtctttaa ctttgctgat cccgatggca
     7501 actactttgc ggttacaggt aaataataga aataaagact ttgtttttct gtgcgaaaca
     7561 ggaaagcgaa gtctttttaa ttcaaataat aatattatgt aaacaaaaaa ctttccatgc
     7621 gtaattatac acaagcaaat aaatcgtcaa atgcgaacgc cttctttata ctgtttagga
     7681 acgaaaagaa ggaaggaaga aatacatgga ttatcaagta ctgctttatt acaattatac
     7741 aaaaatcgaa gaaccagagg aatttgccaa aaatcattta gccttttgca aatctttgga
     7801 tcttaaaggt cgtattctag tagctgaaga aggaataaat ggtactgtgt ctggaaccgt
     7861 tgaaaacacg actgcctata tgaaagcaat gaaagctgat cctcgatttg ctgatacgat
     7921 gttcaagatc gatccaagcg aaggacatgc ctttaagaag atgtttgtaa gataccgtcc
     7981 agagcttgta agtttaaagc tagaagagga cattgatcca aaccaagtga caggtgccta
     8041 tctttcaccg caagaattta aagaagctat tttagatgaa cagacagtgg tgattgatgc
     8101 acggaatgat tatgaatatg acttgggaca ttttcgaggg gctgtacgcc cagatatacg
     8161 cagttttcga gagcttccag actggatccg cgagcacaaa gaagaattta tggaaaaacg
     8221 ggtagtgacc tactgtactg gtggcattcg ttgcgagaag ttttctggtt ggcttgtccg
     8281 tgaaggattc aaagatgtcg gacagctgca tggcggtatt gctacttatg gtaaggatcc
     8341 tgaagtccaa ggagaattat gggacggcaa gatgtatgtg tttgatgaac ggatcgctgt
     8401 cgatatcaac caagtagata aacaaatcgt cggtcgcgac tggtttgacg gaacaccatg
     8461 tgaacgatat atcaattgta gcaatcctga gtgcaatcgt cagattcttg cttccattga
     8521 aaatgaagaa aaacatttag gagcatgtag catagagtgt gcgaaacatc cgcgcaatcg
     8581 ctatgttcaa gaccatggaa tctctccaga agaagcagaa ctgcggttta cagctttaat
     8641 taaatagaaa acaatagttt tgattgtcga ccatttccaa tatgcgctat actagcgttg
     8701 aggatggtga tccacatgaa atcattgatt tatgctattg gggatgtcca tggcaaacat
     8761 gagctttttg aaaaacttat tcactattat gatccaaaga tccatcagct tgttttatta
     8821 ggggatctca atgatcgagg tcctcgtaca aaagaatgtt ttttattagg aaagcaatta
     8881 gtcgaagaaa caggagccat ttacctccgg ggaaaccatg aagaatattt tttgcaattt
     8941 ctgcaatcac cggaggactg gtatgcgcct tatatgagaa atggtggaaa agaaacgttt
     9001 gaaagcctga ttcacccagg ggcatcggag gaatactccc caactgaaat ctcgatgatg
     9061 atccaatcga gatattcaga actgattaaa tttttagtcc agcgcccctt gtattatgaa
     9121 tgggaaaatt atctttttgt ccatgccggt gtggatttga caaagaaaga ctggcgtaag
     9181 acaagtctaa gagactttat ttggataagg gaaccatttc atgaagggaa aaacaataca
     9241 ggtaagacaa ttgtatttgg acatacgatt acaccaatgc tccatggaga tatgcagaca
     9301 acagacctct ggattcagga tcataaaatc ggaatggacg gtggggctgt attcggaggg
     9361 tcattgcatg gggttgtttt tgacaaggaa ggtattcttc aagatattga atatcagaac
     9421 aacgaaggac cttggcatgg aatctgacta atggaaagaa tgaaggagct gtgactttgt
     9481 tgcggctctt tttattttta tagagaatat tgtttctttc agtagtttaa cccaggataa
     9541 aaagtatgat agaataaaga agcaattttt agcagaaaag aggcatttta atgacggaaa
     9601 atttgaacca gacgatcatt cagcttgtac aaaaagaact ttcagactat cgtcctaagc
     9661 agttgactac tgtacttaat ttgttaaatg aaggaaacac tgttcctttt atcgcgcgtt
     9721 accgaaaaga aatgactgga agtcttgatg aagtccagat tcgtgaaatc gaagagcggt
     9781 atgcctatct tgaaaactta gaaaaacgta aaactgaagt gattcggcta atcgatgaac
     9841 aagggaaact gactcccgag ttagaagcag aaatcaccca agccgtgaaa atgcaacaag
     9901 tggaagattt gtatcgtcca tacaaacaaa aacgacggac aaaagcaacg attgctaaag
     9961 aaaaaggact tgaaccactc gcgaagtggc tgatgcagct tacagatgga gaggtgcaat
    10021 ctgaagcaga aaaatatatt gacaaagaaa aagaagtctc ttctgccgaa gaagcgttgc
    10081 acggtgctca tgagattatt gctgaacaag tcagcgataa tgcaaaattt cggacttgga
    10141 ttcggtctta tacgtataac aaaggtatgt atgtcagcaa tgtcaaggat gagcaagcag
    10201 atgaaaaagg cgtatatgaa atgtattatg actttgcaga acccgtccat aaaatggttt
    10261 ctcatcgaat attagctact aatcgtggag aaaaagaaga tattctgaaa gtattctttc
    10321 aagtagatga agcagcaatt ttagcttact tggatcgcca aatagtcaaa aatcctgcct
    10381 ctccttcttc ttcattcgtc agagaagcct atcaggatag ttataaacgc tttattcagc
    10441 cagcaatcga gcgagaactg cgcaatgaat tgactgaaaa ggcagatgag caagcaatcg
    10501 ctattttcgg cgaaaacctc cgtaatcttt tgttgcaacc gccactaaaa ggtaaagttg
    10561 tcctgggatt tgatccggct tatcgtactg gatgtaaact agcagtcgta gatgcgacag
    10621 ggaaagtttt agcaatcgaa gttatttatc ctcataaacc tgcagcgcaa gcaaaaagag
    10681 aagctgctgg accagctttt atccaattaa ttaataaata tcaagtagat atggtagcta
    10741 tcggaaatgg tacagccagt cgggaatctg aactttttgt ggctgagcag ttgaaatccg
    10801 ccgaccataa aacctattac gctatcgtca acgaagcagg cgcttctgtt tactccgcta
    10861 gcgaaattgc acgaaaagaa tttccgcatc tacaagttga agaaagaagt gctgtttcta
    10921 ttgcccgtcg cctgcaagat cctttagctg aattagtcaa aatcgatcct aaagctgtcg
    10981 gagtaggcca gtaccaacat gacgtttctc aaaaacgttt ggctgaacaa ttggattttg
    11041 ttgtggaaac agcggttaac caagtaggtg tggatgtgaa tacagcaagc ccgcagctct
    11101 tgcagcatat atcaggattg aataaaacaa ctgcccaaaa cattgtcatt tatcgtgaag
    11161 aaaacggaga attcaccgca cgaactcaat tgaaaaaagt gccacgctta ggaccaaaag
    11221 catatgaaca agcaatcgga ttcttgcgag tccctggtgg gaaaaacatt ttggacaaca
    11281 caggtatcca tcccgaaagt tattctattg caaaagaaat attaacttca gtccaacttt
    11341 cagaaaaaga gcttggtaca gaagaagcag tagaaaaatt gacacgatta tccgtagaga
    11401 aactggctga aagcctatca gtcggagaag aaacgttagc cgatatttta gctgggctga
    11461 cacaacctgg acgagatatg cgtgatgaga tgccagcgcc cttattacgt acagatgtat
    11521 tgagtatgga agacttgaaa ccagggatgg aattgactgg aacggtccgt aatgtcattg
    11581 acttcggcgc atttgttgat atcggtgtga agcaagacgg acttgtccat atatcgaaac
    11641 tcagcaaaaa attcgtcaag catcctacag atgtcgtgtc agtaggagat atcgtaacag
    11701 tttggataga acaagtcgat acgaaaaaag gcagaatcag tttgacgatg ctttcccctt
    11761 atgaagagta acaatgagga gtaacaaaaa ttgacacaag aagaactgca gcagttagta
    11821 gaagagatat cattaaaaag cttccagctt cctttcaagc accaagcaat gttcaatcca
    11881 cgcttacgta caactggcgg gcgctatcat ttaaacgatc atcatttaga ttttaatgcg
    11941 aagctatttg aagagtcaga cgaagagacg attgcaggaa tcatcaaaca tgaactttgc
    12001 cattatcatc ttcatctagc tggaagaggg tatcagcata aagatatgga tttcaagtat
    12061 cttttgaaaa aaacaggagg gttgcgctat gcaccagcct tgacgaagaa acagataaag
    12121 atggagtatt atcgatgcca agattgttct gccacgatct gccgcaagag aagaatcgat
    12181 gtacgcaaat atcgttgtgg aagatgtcat ggagaaatca aatggattga gacaaaaaga
    12241 gaagacttta atgaagcaca aaaaatcaga tgaaaggata gaaggccttt ggaaaaaagt
    12301 gagcagagga atctaaaaga ctggaatcag ctattaaatt ttttagccgc acctgaagag
    12361 ggaaattttg aggaactgac ggcacctaca cttattttag ctggaaactg tcttcctatc
    12421 ttagctgata aagcagcaca aatgtattta aatggccaag tagatcaatt gtttttggtc
    12481 ggaggggtgg ggcatgcgac tagaatcctt tatgagaatt ttgaaaagca aggatttcat
    12541 tttgaagatg gcatgagtga aagtgaaatc tgtcgtcagt atctaaaaga agtctatgat
    12601 ttgcccgata aggcttttct gatagaatcg aagtctacaa attctggaga gaacgccact
    12661 ttttcactgg agattcttca ttccttagga gctgtaccag aaaaaatctt attgatgaat
    12721 gatccgacac tgcaacgcag gactcgagca acttttgaga aagtatggca aaatgaacaa
    12781 actattttcg cgaatgctgt gccatttgtg ccggaaatcc ttcacttttc tgaagagatc
    12841 atctttacag caaaagaact gaatcatcaa tggcctaagg aatatttcca tgcgctagta
    12901 ttaggagaaa tggaacgtct ccatgacgat gagaacggct atggtccgaa aggaaaagat
    12961 tttattcctc acattgatat atcagaggaa gtttggtctt cttacaccag aattaaagag
    13021 tcaataaaaa cggatttttc tcgtacttga ccattttagc aagcgaatcc tcctttccaa
    13081 ataaatttgg ttgaaatttt caatcgttta taagataatg aggagcgtgt tgaatgtaat
    13141 agctaaaaga atggacgcta gataagagaa gaggattcaa atggaaaatc aaaataaatt
    13201 gtatcatttt gttggtatca aaggttcagg aatgagctcc ttggctcttg ttttacatga
    13261 acaaggtcta aatgtccaag gatcagacat tgaaaaatat ttctttacac aaagagattt
    13321 agaaaaagca aatatcacga ttctgccatt taacgcagat aatgtaaaac cagggatgac
    13381 gatcattgca ggaaatgcat ttcctgattc acatgaagaa atccagcgag caaaagaatt
    13441 aggtttggaa gtgattcgct atcacgactt tattggtcat tttatccaaa attatacgag
    13501 tatcgctgta acaggctctc acggaaaaac aagtacgact ggactgctct ctcatgtatt
    13561 gagtggtgtc cgtccaacaa gttatctaat tggagatgga acaggacatg gtgatccgca
    13621 agcggaattc ttttcatttg aggcctgtga atatcgccgt catttccttg cctattcacc
    13681 agactatgca atcatgacca atatcgattt tgatcatccg gattattata caagtatcga
    13741 cgatgtattt acagctttcc aaacaatggc tggacaagtg aaaaaagcta tttttgcgta
    13801 tggtgacgat gcttatttac gcaaattaaa agcaaatgtg ccgatttatt attatggtgt
    13861 cacagaaaac gatgatatcc aagcacgaaa catcgaacgc acaacttctg gttctgcttt
    13921 tgatgtctat catggggatg aatttgttgg gcacttcact gtaccagcat tcggtaaaca
    13981 caatatctta aatgctttag gtgttattgc agtagcttat ttcgaaaaac tggatctgaa
    14041 agaagttgca gaagaaatgc tgactttccc aggagtaaaa cgtcgattca gtgaaaagat
    14101 cgtagcagac atgaccgtag tcgatgatta tgcgcatcac cctgcagaga tcaaagcaac
    14161 gattgatggc gcacgccaaa aatatccaga taaagaaata attgcagttt tccaacccca
    14221 tacgtttacg cgtacgattg cattgatgga tgaatttgca gaagcacttg atttagctga
    14281 taaagtttac ttatgcgata tttttggttc ggctcgggaa gaacaaggaa acgttaagat
    14341 tgaagacctt ggagcgaaaa tcaaaaaagg tggagaagtc atcaaagaaa ataatgtatc
    14401 tccattgtta gattatcatg atgcagtagt aatcttcatg ggtgctggag atgttcaaaa
    14461 atttgaacaa gcgtatgaaa aacttttaag cagcacgacg aaaaatgtac tgtaatacaa
    14521 ttactgcagt gtccttataa aaatagaaag agggggagtg gcaaaaatct tgccaagccc
    14581 ccttttttgc aacttatgat aaacggtgtt caaaagcttg catctgcggt aataagccca
    14641 atcaaatcaa aaacatgaga agctgttttt tcttggctgt tcttattatt tgatgcagga
    14701 cagctttttc acgacctctg taaactatag gaatctgtta aatcaaggga gcagaacttg
    14761 ccttttacgc tacttttggt acaataatgt atgattaaaa ataaactgga gggtttctat
    14821 cttgaacata ggtaagccga gaaaactagg aaaaacaata aatgaaatcg aggaaggcga
    14881 ctcgttatcc ttaacagaat ctattgaaga taaccagctt ttgctgtatt tgggattgac
    14941 gaatgatgca aatcctttgt atatccaaca tgactttgca caaaaaacag aatatcaaca
    15001 gccaatcgtt ccatcagtga tgctgatggg gatcatcaca agtgcaattt caaagcatct
    15061 tcctggacca ggatcacatg ttgtcaattt ttctgtcaat tttattgaac cggttttcca
    15121 ttatgaaaca ctgacttttc agtttgaggt cataaaagta gataagatga aggatgtggt
    15181 gacgatttct gtagaagcgg agaatacgaa cgaagatcgt gtgttggatg ctgtcgttat
    15241 ggtccaaccg ccacaagttc ctgaattgga aatagcaatc gaaagtgaag gtgaattgaa
    15301 tgaataatcc aagtacatca atgtcaggta tcaatcgttt ttatgcaaaa gtttatggat
    15361 ttttaggtat gggacttgcc cttagtgcag tcacttctta cttggctctt tgggtatttc
    15421 ctttgcaatt agcaactttt atcaataatt ttccactagg ctttgtgggt ctgtggatcg
    15481 ttgaactgat tttagtaatg gttctaggcg taaaagcaca aaagaatccg agcttagcaa
    15541 tcggtggatt tattgcctac tctcttttga atggcataac attagcggtt actcttgcat
    15601 actacgatat tggaacagta acgcaagctt tcttgacagc tactttgatg ttcatgggga
    15661 tgtctgttgt cggagtaaca acaaaaagag atcttagcgg ggttggaaga gcaggacagg
    15721 cagctttaat cggagtgatc attgcactag tcttgaacat cttcattttg agaagtagcg
    15781 tagttgagct gttcatctcc ctactgttag tcgtcatctt tgcagggatt acagcttatg
    15841 ataaccaaag catccgtaat atctatgtcc agacaaacgg aacagcacat agtggaattg
    15901 ctgtattcat ggcattgaag ctgtatttgg actttatcaa cttgttcttg gcgtttctga
    15961 gaatctttgg acggaacaac taaaaaagaa aaaaattcat cagttggggc tataccgaat
    16021 tatttggtat ggtcccgctt tttcttttcg cattccgcag gattctttgt tagaatgaat
    16081 aagaacttta cagtaaggag ttaagaagat gacaaaaaat aaattattac tcgttgatgg
    16141 gaacagtgtg gcttttcgcg cattctttgc gcttcacaat tctcttgaac gtttcaaaaa
    16201 tagaaacggt cttcatacaa atgcaatcta tgcattcaat acgatgtttg aaaatgttat
    16261 gcaaaaagag caaccgactc atgttctggt tgcgttcgat gcaggaaaaa cgacttttcg
    16321 gacagaaatg tatgcggagt acaaaggtgg aagatccaaa acacctggcg aattcaaaga
    16381 acaaatgcca tatatacgtg atttgttgac aggactaggg gtacagtatt acgaattgcc
    16441 caattatgaa gcagatgata ttatcggtac tttggcagaa aaagtagaca aagatcaatt
    16501 tgatgtcgtc gtgctttcag gtgatcggga tttgactcaa ttagctagta aagaagtaaa
    16561 agtggatatc actgtaaaag gtgtcagcga tatcgaaagc tatacaccag aacatgtagc
    16621 ggaaaaatat gatgggttga cgccaaaaca gattattgac atgaagggac tagccggaga
    16681 tgcctctgac aatatccccg gtgtgactaa aatcggtgaa aaaacagcta tcaagctatt
    16741 gaaacaatat ggctcagtcg aaggaattta tgaacatatc gatgaaatga agcaaagcaa
    16801 aatgaaagaa aatctgataa atgacaaaga gcaggctttc ttatcgaaaa aattggcgac
    16861 catcaatact tcagcgcctg tggatgtctc tatcgactca ttgaagtatg aagggaaaaa
    16921 tctcgataaa ctcgttccgt tttataaaga aatggatttc aatcagttcc tttctaaatt
    16981 gaatatcgta gaagaagaag taaaaatgga tgatattttg tttgaagtcg ttcatgaatt
    17041 caaagaggaa atgtttacta cggatatggc gttgtacgtg gaaatgatgg gcgacaatta
    17101 tcatacagag gaaatcgtcg gagtagcgtg ggggacagaa aagaaaattt atgtcacgaa
    17161 cgatctatct gtattcgaaa accgagcgtt ccattcttgg atcactgatc cgactcggct
    17221 gaaaaaagta tatgatgcga aacgaactta tgttgcttta aaccgctata ctggaaaaac
    17281 gaaaggaatc gattttgatg tcttgctagg tgcatacctt ctcgatacga atgaaaaaag
    17341 cacagatatc gaaggaatag ctgctcatta cggatacaac gatatccaat cagatgaagc
    17401 tgtatacggc aaaggggcga aaaaaggttt accagaagaa gaggaactct tttttgctca
    17461 tttagcgcga aaagtagctg caatcaatgc gttgacacca aaattgagcc aagaactggc
    17521 agaaaaaaac caagaagatc ttttccggaa aatggaactg ccattgtcta tcattttggc
    17581 agagatggaa ataagcggga tcaaagtaga tgcgactcgc ttacaagaaa tgaagggcga
    17641 gttctctgct cgcttaagag agatcgaaca aaaaatctac gaagaagccg gcgaagaatt
    17701 caatttgaat tcaccaaagc aattaggagt gatcttattt gaaaaaatgg gattacccgt
    17761 tatcaaaaaa acaaaaactg gatattccac agccgtagat gtactggaac agcttcgtga
    17821 acaagcacca atcgtagagg atatcctcac ctatcgtcaa attgcaaaaa ttcaatctac
    17881 ctatgtcgag ggactgctga aagtgatcca atcagatgga aaagtgcata cccgttatgt
    17941 ccagacattg acccaaacag gacgattaag ctctgttgat cctaatttgc agaacatccc
    18001 gattcgatta gatgaaggga gaaagatacg tcaggcattt gttcctagag aaaaagactg
    18061 gctgatttat tcttctgact attcacaaat cgagttacgc gtacttgcgc atatctcaaa
    18121 tgatgaacat ttgaaagctg ccttcctaga aggtcaagat atccatgcca acacagcgat
    18181 gcgtgtattt ggaatcgaaa aggcagagga tgtcacacca aatatgcgta ggcaggcaaa
    18241 agcagtgaat tttggtatcg tgtatggcat ctctgattac ggactttcac aaaatctagg
    18301 aatcagccga aaagcagcac agcaatacat cgatacgtac tttgaaaaat acccaggtgt
    18361 caaagaatat atggaaagaa tcgtacgcga agcaaaagac caaggctacg tcgaaacgct
    18421 gtatcaccgc cgtcgatatc tgccagacat caattcaaga aattacaata tccgttcttt
    18481 tgcggaacga acagctatca atacaccaat ccaaggaagt gctgctgata ttttgaaaat
    18541 cgcgatgatc gaattggata aacgattaaa agaaacaggt cttcaagcta cgatgctgtt
    18601 acaagttcat gatgaacttg tcttcgaggt accggaaaaa gaacttgaat cattagataa
    18661 attggtgaag gaagttatgg aacaagctgt ttctcttcat gtaccactga tcactgacag
    18721 cagttgggga aaaacgtggt acgaagcaaa ataaacattg tttaagacag agcaatataa
    18781 tgaaggtgaa aagaaaaaat gccagaatta ccagaagtag aaaccgttcg taaaggattg
    18841 gaacggttag tagtaggaaa aacgattcaa aaagttcagg tcttgtggcc caagatcatc
    18901 gagcaaccag agacacccat ctttgaggct tctcttgttg gcgaaaccat ccaatccatt
    18961 ggacgtagag ggaagttttt aatttttcat ctcgatcatt gtgaactgat ctcacacttg
    19021 cgaatggaag gaaaatatca atttacgaaa gaaaatacac caatcgataa acatacgcat
    19081 gtacttttct tttttgagga tggaagccaa cttcgctaca acgatgtacg aaaatttggt
    19141 cgtatgacaa tcgtcgaaaa aggagcaagt gcaacttacc gaggaatcat gaaattggga
    19201 cctgagcctt tgccagatag cttcttatta gcagatttcg caaatggact gaaaaagtcc
    19261 cacaaagcaa tcaagccatt gctgctagac caacgtctag tgactggtct aggtaatatt
    19321 tatgtagatg aagcattatg ggaagcgaag atccatccag aacaaccggc gaacacactg
    19381 cgccccaaag aggtagaaca attacgtcta tcgatcattg atgttttgga tcgtgcaata
    19441 gaagccggag gtaccacgat tcgcagttat ttgaatgcat taggagaatc aggcggattt
    19501 caagtagcac tgcatgtcta tcagcaaact ggcaaaccat gtatacgttg tggaacgccg
    19561 atcgtgaaaa caaaagtggc acaaaggggg acgcactatt gtccgaattg tcaaaggcta
    19621 aaaatcgcaa agtaggattt gttttaggtt tgacgggtgg aatagctacc ggtaaaagta
    19681 cggcagccaa agtctttcag tctcatggat ttcctttgat cgatggcgat gtgattgcca
    19741 gagaaactgt tgagcctgga acgcttgctc tgaggaaaat tgtatcagtg tttggccaag
    19801 aggtattgca gcctgatggg caactagatc gagggaaact tgggatgatt gtttttccat
    19861 caaaggaaaa acggcaaaaa cttgatcagc tattagatcc gtttatccga aaagcgataa
    19921 aagatcaaat cgcctctcta agttccaatc atcctttagt aattgtagat atcccattgc
    19981 tctacgaagg tcattatgac cactatatgg atgcagtagc tgtcgtttat actacgccag
    20041 aaactcagct tcagcgtttg atgaaacgta atcaactgac gctagaacaa gcgcagcaga
    20101 gaattagtag tcaacttcca atcgaagaaa aaaagcagcg tgcggatatt ttgttcgaca
    20161 acaatggaac aaaagaaaaa cttgtgggac aaatcgaaaa ttggcttttt gctaatcgct
    20221 ttctttgagt atctgtgtca attttgctat cctgagtata tagaaagtga gggatagcga
    20281 tgggatcatt aaaaaatcaa aaggataaag tcgtaggtaa cgcaaaagaa aaaatcggcg
    20341 agatgctcaa taaagaaaat ctaatcgatg aaggtcgtac gcaatcagaa acagcagaag
    20401 aaaaagaaaa gcagtaccaa gcaagaatcg atgaaataga agaccagcag gcagaagaag
    20461 aagcgaagcg gcaagatttg actcaagaac gaaaaaatcc gacacctgat cgtagtaaaa
    20521 caccgctccc agaaagtgac cacattcccg atgagcaaaa aatcgaggaa cagcgaatga
    20581 aacactatac aggaatcgaa gagaagttat aaaatagtaa aaaatcgaga tgtgacttga
    20641 agcagatgag aaggtgttca tctgcttcaa gttttttgtg ctagatgact aaatcagctt
    20701 ttgtgtaagt tatttctcag taatcaaggt gatcagtatt ggaaaattga aatttagtaa
    20761 ggacagggga cacaattacg catgaaagta gaaaaaattg ttcttatatc cgtgtttttt
    20821 gggtaggaca gtcgcgtttg tgatttgttt cgtgttataa tacaactagt tgtaaagaaa
    20881 gacacatcct ttatataaaa tgcgatgcaa gaggtgagac aaaatgagat gtcccagatg
    20941 tcaacataat aattcacgag taatcgacag ccgtcaagca gatgatggac gtgcgatccg
    21001 tcgccgtcgc gaatgtgaga attgttcgtt ccgattcact acatttgaac gaattgaagc
    21061 tgctccgtta ttagtaatca agaaaaacgg ggaacgcgaa gagttcaatc gcgataaaat
    21121 tttaagagga ctcatccgtt cagcagaaaa aagacctgta gctatggaac aaatggaaca
    21181 aatcgtcaac catgtcgaaa accgcgtacg cagtctcgga gaaaacgaag tatcgacgaa
    21241 tctaattggt gaatatgtga tggaagattt agttgattta gacgaaatcg cttatattcg
    21301 ttttgcaagt gtttatcgcc agttcaaaga tatgtctgtt ttcttaaaag agttgcaaga
    21361 tatcgtggat aaagcaaaag aaaataataa agagaataat aaagaaaaca attaagaaac
    21421 aaaaaggagg catttccttg gaaaatgctt ggaaagagct tcaaccaaaa aatatttacc
    21481 aaggaagaaa agcggctccg ctgagagaag acagcaagaa cgccttgctg tatctttatc
    21541 agccactaat cggcggagaa gccttgagta tctatttcac cctgttatca gaaatctctc
    21601 aagaatctgg caaaggtcct gaagggatgc atgcagatct gcttagtagt ctaggctgcg
    21661 gtatcccgca attttatgat gcaagaaaaa agctagaagg aatcggctta ctggatgttt
    21721 attttaaaga agatagtgat ctgggacctc gttttattta tgagctgtta gaaccactca
    21781 ttcctaaagc atttttcaaa gactctgttc tggcttttct tcttctggaa aaagttggtg
    21841 aacgacgatt ccagaagctt acccaattct ttgaacctaa aaggcataca gcagagggat
    21901 atcaaaaaat cacaaaaaaa tttttagatg tttatcgctt caatgaagca gtgtacgctg
    21961 ctaatcaaga aaacatcgaa tcgctgaata aatcttttga agaatctgaa tcagtagcat
    22021 tacttgaagc agattcgaca ttcgattggc agtttttgaa tgattggctg gcaaaaaaac
    22081 atattgcaag tcttgatgat ccaatcatca aacaagtcga catgtatcat cagctttacg
    22141 gtttcgatga attgacactt ggcgaactga tcagtcaatc ttatgacttt agtgaacaaa
    22201 aagtatctga aaaagaactg caaaaagcca ttttggcaat gcagagacgt ccggtgaagc
    22261 aaacaaaaga aacagttgcg gtttctgaaa acagtaatga accctcgtca aacgaacatt
    22321 tgacaaacgc agcgcttcag ctgataaaag aagcacagag cattccaccg atgaaatatc
    22381 tggaagcaat caagtcagaa aaaggcggat atgtttctaa acaagaaaac tggttactgc
    22441 aagatcttgt ttctcaatca ggtctttcca gtagcgtgat caatgttttg ctgaattatg
    22501 tactagtgat caagaataat gctagcttaa atgccagtta tgtaaataca gtggcaaatg
    22561 aatgggcaca aaagaaaatc gccacagcag aagaagcgat ccagcacatt cggcaaataa
    22621 gtaatcaagc aaaacaatca aaacaaaaga agcaaacaaa ttatcaaact ggcaagcgaa
    22681 atgttcgacg ggaaaagctt cctgattggg tcaaccagcc aaaagatgaa aaacagatca
    22741 gcagcgagaa aaaagcagag attgaccgcc gttttaaaga atacctggca aaaaaggagg
    22801 gtgatatcta gtggaagacg taggcaaaga attgaagagg atcatcagca aacgcaatta
    22861 tcaagaaaag tatgctgaaa tgatcgctga agtcttaaat gatgcggatg ttcaagcttt
    22921 tctaagcgca caccaagaag aattgacaca agcggatatt gaaaaaagtt acgctaaatt
    22981 gtatgaattc gtgcaagaaa agcgtaaata tcaaaaaaac gatccaacga tgatcgctcc
    23041 cggctatgag ccgaagctga cgttgaattt tcattatgtg gatgtcactt atgtaccaac
    23101 ggaagcattg ttagcacggc aaagacaaga agaaatcaga agccgagtaa aagcaatgga
    23161 tatgccaaaa gatattcaat cggcttcttt tgaaaacttt gaacgtacag atggacgagg
    23221 atatgcgagc ttagaagccc tagattttgt agagaaatat actgctgatc caaaagggta
    23281 tcataaagga ctgtatctag taggaagttt tggtattgga aaaacatatt tattaggagc
    23341 aacagcgaga gaattagcga ttgctggatt caccacgacg ctagtccatt ttccgacatt
    23401 tgccgttgag atgaaacagg caatcggaaa agatcaagtg gcagaaaagt tagatgcagt
    23461 gaaacgttca cctattttga tgatcgatga cattggtgca gatgcgatga gtagttggat
    23521 acgagacgaa gtattcggtg ttatccttca atatcggatg caggaacagt taccaacctt
    23581 cttctcatcg aattttacaa tggatgaatt ggaacagcat ttgtccgtca ctcaacgtgg
    23641 agatgaagag ccgctaaaag cgaaacggat aatggaacgc atacgttatt tgacaaaaga
    23701 aatcgaaatg actggtcgca accgacgcaa tggttgaaca gttgacaagt ccaactgaca
    23761 tttgacctag tagtaaaagc aggtcagcga atcattatat ggacagaaat gaccggagac
    23821 aatcaccgca acaagtaatg cagaaactta aacggaggat tttttatgag ggctattttt
    23881 tctagtatat ctgtagttgg tctatttttt cttttagcag gctgcggtag cgcagatagc
    23941 aacacaggtt ctgttgattc gattgatgcg ggtcaaacag taaaaacaag cagtacgaaa
    24001 acggagagac aaagcatatt tgtcggaacg attgaaagta tcactcaagt agaagcagaa
    24061 gaagaaacgc ttcagttgaa actcgtcgat gtcatggcca aagaagatcc agcgaatatg
    24121 ggaacgtcat tcagcaatga cggagtgatc ttaaatgcta ctgctgaaaa aataagcgtg
    24181 gacataaatg aattgaaaat cggtgacaaa gtccaattta cattagttga gatgccgatc
    24241 atgacgatgt ctatcccacc gcaagttcct ggagattcga ttattgatgt ttcattgaac
    24301 aaataaccaa taacattttt tcaaaaataa gaaaatgttg ttttatagaa cgaaaaggta
    24361 tattttttaa ggagatatgg gaattttttt attcctagtc tccttatttt atttggaaaa
    24421 tgctataatt atctagaagg agtgaagcta tgaaccattt agatttatct cagcgcttag
    24481 ccgcagtggg tgactttgtc cctgaatccg ctcgtttagc agacattgga tcagatcacg
    24541 cttacttacc cgttgcattg atattaagag gaaaaatcga atatgcagtt gctggtgaag
    24601 tcgttcaagg tccttatgaa tcagcacgta agcaagtcaa aaaagatgga ttagaagacc
    24661 gtatcaccgt acgccttgca aatggtctag atgctatcca gccacaagat ggtattacag
    24721 ctgtcacgat tgcgggtatg ggaggttcac tgatccgaga tatcctagaa gctggcaagt
    24781 tgaatcaacg cttaagcgga caagaacgtt tgatccttca gccaaacatc ggagagcgta
    24841 cattacgatc ttggctgcaa gaaaatcagt ataaaatcca atcagaaaaa atacttgaag
    24901 aaaataaaaa aatatatgaa atcattgtag ccgaaaaaga acaaaaccca gttacttaca
    24961 atgaaatgga gctgatgttc gggccatttc tgcttgaaga gaaaagtgag atcttcaaat
    25021 caaaatggac aagagagctt caacaaagaa aagctatccg tgaacagctc aaacaagcag
    25081 ctaatcaaga ggcacgtcaa aaacaaatcg agcaggaaat ccaaatgatt gaggaggttt
    25141 taaagccatg acaatcagcg gacgcacttt catccaaaag tttgaaagct attgtcctca
    25201 gtggctagct gaagaaggag acccagtcgg actgcatatc ggaacattgg ataagccaat
    25261 ccaacgagta atgatgacat tagacgttcg tccagaagtg gtcgaagaag caatcaagaa
    25321 aaaaatcgat ttgctgattg ctaaacatcc tccaatcttt cgtcctgtaa aacggctggt
    25381 tactgatcag ccacaagaaa aaatgtatgc agatttgttg aaacatgata ttgccgtcta
    25441 tgcagcacac acgaacatgg atattatttg ggatggactg aatgactggt tttgtgaact
    25501 tctcggtatt gaagtggaga gttatttagt aaagactcat gagattcatt ataaaaaact
    25561 ggccgtttat gtacctgtgg atcatgcaca gaaaatgcgt gaagtattag ctgcagctgg
    25621 tgctggtaca caaggtgatt ataccggtac aagttttacc tcgattggac acggtcgatt
    25681 tactccggaa gctggtgcac aaccagcgat tgggaaagta gggaagacag aacaagttca
    25741 agaggcaaaa gttgaggtta ttttgccgga aactatcgaa aaacaagtga ttcaagccat
    25801 gcggtcagct catccttatg aggaacctgc ttatgatctt ttcgctatag atgaaccagt
    25861 cgaaatgttt gggttaggac gagtaggtga attgccgcaa gaaatttcta tagaggcatt
    25921 tgtagaacaa gtaaaagaag cgttccaact agatggactg agaatagtcc agccaaaaaa
    25981 tgcaaaatcc tctgtgaagc gcattgcgat ttgcggtgga agcggtgaaa aattttatcc
    26041 gcaagcaatc gcccaaagag cagatgtcta tatcacggga gatatctatt atcatacggc
    26101 tcatgatatg caaagcgctg ggttgattgc aattgatccc ggtcattata ttgaatcgtt
    26161 atgcaagcaa cgatttatcg agaaatttga atcttggaaa caagaagaaa attgggatat
    26221 taactttttc gtttctgaaa caaatacaaa cccatttcaa tttaattaaa aggagagaat
    26281 actatgtacg aaaatctttt gccacgtttt ttacgatatg tcaaaacaga aacacgctcc
    26341 gatgcaacaa gcaaaacgac tccatcgact cagacacaag ttgcttttgc gcaagaattg
    26401 aaaaaagaat tagaagagtt aggcttagct gatgttttct ataatgaaga taacggattc
    26461 gttatgggaa cattaccaag caatgttgat catgaagtgc gttccattgg atttatttcc
    26521 cacatggata cagcagattt taatgcagta ggagtcaatc cacaaatcat tgaaaactat
    26581 gatggcgagt ctacgatcgt actagatgcg gaagaacgct atacattgaa tacaaaagat
    26641 tttccaaatt tgaaaaatta tgaaggccac acattgatca caacagatgg cagtacatta
    26701 ctgggagcag atgacaaatc agggattgcg gaaatcatga ctgcaatgga gatcttgatc
    26761 aataatcctt ctatccctca cggtaccatc aaagtggctt ttggtcctga tgaagaaatc
    26821 ggtgtcggag cggataaatt cgatgttgag caattcgatg tagattttgc ttatacagtc
    26881 gatggtggtc ctttaggtga gttgcagtat gaaacgttta gtgctgctca agcaaatatt
    26941 acgatccaag ggaaaaatgt acatccaggg acggcaaaag atacaatgat caatgctcta
    27001 cagttagcaa tcgattttca taatcagctt ccagccgatg aagttccaga aaaaacggaa
    27061 ggatacgaag gtttcttcca tttgatggca ttggatggga atcctgaaga agcttctatg
    27121 agttatatca ttcgcgatca tgatcgtgaa aaattcgaag cacgcaaagc acaaatcaca
    27181 catatccaag aagagttgaa tcagcgattt gatcaagaac gaatcaaagt agatatgtat
    27241 gatcaatatt acaacatgaa agaaatcatt gaaaaagata tgagcatcgt ggaattagcg
    27301 aaacaagcaa tgatcgagct tggtatctct ccagtgatcg aaccagttcg tggaggaaca
    27361 gatggttcaa aaatctctta catgggtatt ccgacgccaa atctatttgc cggaggagaa
    27421 aatatgcacg gacgattcga atttgtttct ttacaagtaa tggaaaaagc aacagacgtg
    27481 attgtaaaaa ttgcagaatt gaacacaaag agttaaaatc gaaaaagaac agcttatgac
    27541 aaggcgttcg tcccaatgat ggaatgtcat ctgtctaagg ctaaagggca ggacaatgga
    27601 attggttgtc ccgccctctt ttttgaagag taaataggag gtaaagaatg gaaaagctgc
    27661 tagtttttta taacaaaagt tcagggaaag atgagggtga gaaattagca cgctggtttc
    27721 aagattatgt aaaagaaaag cagccagaat tatatgtaga actagctgaa actggcccat
    27781 cgattgatga tgcggaatta gtcaagaaag cagaagccat agaagctgat actttagtaa
    27841 ttataggtgg agacggcacg attcatcata tcgtccaagc gttccaaaaa acattatctc
    27901 aataccatgt cggattactt ccaggtggaa ctgtcaacaa ccttgcacgt gttcttgaga
    27961 ttcccctcaa aaaagaagaa gctgctgacg tgatcctgga aggcaatgta cggaaaatag
    28021 actatggtca agtcaatgat gaagtcatta tctctacact gacaatcgga atcttagcag
    28081 atacagcagt ttggatcagt caagaagaaa aacagcgtta cggctcgtgg atctttatca
    28141 agaagttttt taagttattg ctgaagaaga aaaaatatca tttggattta gaaacagacg
    28201 aggagcattg gaaaggaaaa tcccaactgt tgacagtcac gatgtctaac tccgtgggcg
    28261 gttttacaaa ttttgacgac tcagcgaccc tagatgatgg aaaatttcat gtaacgatcg
    28321 taccttcctt agatattttc cgatttattt tttatttgcc tagaatcatg agaggcaaat
    28381 tctatacgat ccccggcatt acttatttca cagccaaacg catgaaaatc caagcgcagg
    28441 aaaaatctgt gggcactcga acggatggag acacgacaga tgatcttcca gttgaattat
    28501 ctgttgtatc aaaaggatta acagtttatt caccaaagaa agaagaaaaa gcttagaaat
    28561 agtagttaac agaaagttgg aagaagagta atggatgtat tgttacacaa tcctttgtgg
    28621 ttttaccttt tcattgtgaa tttttactta ttttgtctga tggggtatga taaacatcag
    28681 gctagaacta gcggctggcg tgttcctgag aataatttga tatttgtcag cctgttaggc
    28741 ggtggtttgg gaggattgat ctctcgaaaa gttttccatc ataaaactcg gaaaaagaag
    28801 tttactatag gattcgctgc aggtgtactt gttgatttga ttctcattta ttttttccat
    28861 taaggagcag gagatgtcgt ctaaaggaac gaaaactaaa atcattctga atctgctttt
    28921 gctgattgtg atttttgcca ttatttatta tttaatcaat caatcatttg cagatatctt
    28981 tgccgaattg ctgtctacca gtgttgttgt tttggcagcg gctgttatat tagggacagt
    29041 ttaccaaatc gctgaagggc gctcaatcaa agaaattgct gaacctttcg cgccgaattt
    29101 tacaacgaaa gatgggttct tgacctctag ctatattgcg ttttaccgaa tcgtttcgtt
    29161 tggtacagga acgctgatct cggagattta tttttataac aaaaaaggaa tgaagctatc
    29221 ccagggagca ggggtgacag cactccatat gattatgtac aaactggcag tgatcactta
    29281 tgcattagct ggcttggtca tccaattctc tcttttttat tctaatggtc catcaatgat
    29341 ctggtttatc atcgctggta tccttttgac gatcgtgatt gttgcctttt tattggcgat
    29401 gtccgtcagc ttgaatgttc aaatattttt tgtcagctta tcgaataagt tatttaaaag
    29461 tgagcgtttg cggaaatggg tggatacatg caatcagcag atctactctt taagggaaac
    29521 ggtccaaacc attatccaag acaggactgc gctattgcgg atttatctat ggaatatgtt
    29581 aaaacttgta ttttggtatg tacttcctta tttgattctg attgaaaacc atcctaaaat
    29641 agattttcta ctgaccattt cgtttatcag ctttgcagta gtgctttcag gggttattcc
    29701 tacgcctgct ggtattggtt cctttgaatt tgtctatttg ttattattca gaccagttgt
    29761 tggaacagtt gacgcagttt cttcgctttt actgtatcgt ttcagttcct ttatcttgcc
    29821 atttctctat ggatttgtgt atgtgattgc tgaacggcga agagtgatca aagaagaaat
    29881 aatagaagta aaaaaagaga ataaagaaaa tcaagggtag gtcttttata agatctaccc
    29941 tttttatcaa atcaaagaa
//
LOCUS       DAYYAO010000031        29097 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000031 DAYYAO010000000
VERSION     DAYYAO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.032"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHARI_001262"
     CDS             complement(<1..1031)
                     /locus_tag="ACHARI_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8442319.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHARI_001263"
     CDS             complement(1113..1802)
                     /locus_tag="ACHARI_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8442320.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHARI_001264"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHARI_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8442321.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHARI_001265"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHARI_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8442322.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHARI_001266"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHARI_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8442323.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHARI_001267"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHARI_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8442324.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHARI_001268"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHARI_001268"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8442325.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHARI_001269"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHARI_001269"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8442326.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHARI_001270"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHARI_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHARI_001271"
     CDS             complement(7636..8523)
                     /locus_tag="ACHARI_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8442327.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHARI_001272"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHARI_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8442328.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHARI_001273"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHARI_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8442329.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHARI_001274"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHARI_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8442330.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHARI_001275"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHARI_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8442331.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHARI_001276"
     CDS             complement(10454..10957)
                     /locus_tag="ACHARI_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8442332.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHARI_001277"
     CDS             11554..12519
                     /locus_tag="ACHARI_001277"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8442333.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHARI_001278"
     CDS             complement(12618..13790)
                     /locus_tag="ACHARI_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442334.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHARI_001279"
     CDS             complement(13777..14922)
                     /locus_tag="ACHARI_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442335.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHARI_001280"
     CDS             complement(14936..15868)
                     /locus_tag="ACHARI_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442336.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHARI_001281"
     CDS             15985..17064
                     /locus_tag="ACHARI_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8442337.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHARI_001282"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHARI_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHARI_001283"
     CDS             complement(17789..18016)
                     /locus_tag="ACHARI_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8442338.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHARI_001284"
     CDS             complement(18119..20707)
                     /locus_tag="ACHARI_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8442339.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHARI_001285"
     CDS             complement(20727..21386)
                     /locus_tag="ACHARI_001285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8442340.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHARI_001286"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHARI_001286"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8442341.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHARI_001287"
     CDS             complement(22159..23025)
                     /locus_tag="ACHARI_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8442342.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQLVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHARI_001288"
     CDS             23134..23775
                     /locus_tag="ACHARI_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8442343.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHARI_001289"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHARI_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8442344.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHARI_001290"
     CDS             complement(25216..26115)
                     /locus_tag="ACHARI_001290"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8442345.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHARI_001291"
     CDS             complement(26117..26914)
                     /locus_tag="ACHARI_001291"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8442346.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHARI_001292"
     CDS             complement(26892..27596)
                     /locus_tag="ACHARI_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8442347.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHARI_001293"
     CDS             27727..28302
                     /locus_tag="ACHARI_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8442348.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHARI_001294"
     CDS             28420..29097
                     /locus_tag="ACHARI_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8442349.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6166 c         5231 g         9137 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgctttaaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacaagt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaa
//
LOCUS       DAYYAO010000032        28621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000032 DAYYAO010000000
VERSION     DAYYAO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.033"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..1103
                     /locus_tag="ACHARI_001295"
     CDS             78..1103
                     /locus_tag="ACHARI_001295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8442350.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            1142..2092
                     /gene="manA"
                     /locus_tag="ACHARI_001296"
     CDS             1142..2092
                     /gene="manA"
                     /locus_tag="ACHARI_001296"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8442351.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            2186..3889
                     /locus_tag="ACHARI_001297"
     CDS             2186..3889
                     /locus_tag="ACHARI_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8442352.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            3891..5915
                     /locus_tag="ACHARI_001298"
     CDS             3891..5915
                     /locus_tag="ACHARI_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8442353.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKADFSRKKLSEEEIAYMVLHFANS
                     LERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQEEYD
                     VVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMIEDT
                     YEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRLLKR
                     YEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVMLAP
                     SPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            5926..6366
                     /locus_tag="ACHARI_001299"
     CDS             5926..6366
                     /locus_tag="ACHARI_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8442354.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(6566..7693)
                     /locus_tag="ACHARI_001300"
     CDS             complement(6566..7693)
                     /locus_tag="ACHARI_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442355.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(7696..8373)
                     /locus_tag="ACHARI_001301"
     CDS             complement(7696..8373)
                     /locus_tag="ACHARI_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8442356.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            8637..10403
                     /locus_tag="ACHARI_001302"
     CDS             8637..10403
                     /locus_tag="ACHARI_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8442357.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            10418..10855
                     /locus_tag="ACHARI_001303"
     CDS             10418..10855
                     /locus_tag="ACHARI_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8442358.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            10868..12019
                     /locus_tag="ACHARI_001304"
     CDS             10868..12019
                     /locus_tag="ACHARI_001304"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8442359.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(12081..12779)
                     /locus_tag="ACHARI_001305"
     CDS             complement(12081..12779)
                     /locus_tag="ACHARI_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8442360.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(12772..14124)
                     /locus_tag="ACHARI_001306"
     CDS             complement(12772..14124)
                     /locus_tag="ACHARI_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8442361.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(14347..15747)
                     /locus_tag="ACHARI_001307"
     CDS             complement(14347..15747)
                     /locus_tag="ACHARI_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8442362.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15908..16645
                     /locus_tag="ACHARI_001308"
     CDS             15908..16645
                     /locus_tag="ACHARI_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8442363.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            16770..17249
                     /locus_tag="ACHARI_001309"
     CDS             16770..17249
                     /locus_tag="ACHARI_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8442364.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            17246..18013
                     /locus_tag="ACHARI_001310"
     CDS             17246..18013
                     /locus_tag="ACHARI_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8442365.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            18243..18812
                     /gene="lepB"
                     /locus_tag="ACHARI_001311"
     CDS             18243..18812
                     /gene="lepB"
                     /locus_tag="ACHARI_001311"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8442366.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(18916..19518)
                     /locus_tag="ACHARI_001312"
     CDS             complement(18916..19518)
                     /locus_tag="ACHARI_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8442367.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            19624..19854
                     /locus_tag="ACHARI_001313"
     CDS             19624..19854
                     /locus_tag="ACHARI_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442368.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            19845..20093
                     /locus_tag="ACHARI_001314"
     CDS             19845..20093
                     /locus_tag="ACHARI_001314"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8442369.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            20199..20783
                     /locus_tag="ACHARI_001315"
     CDS             20199..20783
                     /locus_tag="ACHARI_001315"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8442370.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            20786..21859
                     /gene="prfA"
                     /locus_tag="ACHARI_001316"
     CDS             20786..21859
                     /gene="prfA"
                     /locus_tag="ACHARI_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8442371.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            21852..22691
                     /gene="prmC"
                     /locus_tag="ACHARI_001317"
     CDS             21852..22691
                     /gene="prmC"
                     /locus_tag="ACHARI_001317"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8442372.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            22684..22863
                     /locus_tag="ACHARI_001318"
     CDS             22684..22863
                     /locus_tag="ACHARI_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442373.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            22885..23910
                     /locus_tag="ACHARI_001319"
     CDS             22885..23910
                     /locus_tag="ACHARI_001319"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8442374.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            24085..25329
                     /gene="glyA"
                     /locus_tag="ACHARI_001320"
     CDS             24085..25329
                     /gene="glyA"
                     /locus_tag="ACHARI_001320"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8442375.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            25482..26429
                     /locus_tag="ACHARI_001321"
     CDS             25482..26429
                     /locus_tag="ACHARI_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8442376.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            26563..27192
                     /gene="upp"
                     /locus_tag="ACHARI_001322"
     CDS             26563..27192
                     /gene="upp"
                     /locus_tag="ACHARI_001322"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8442377.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(27331..27573)
                     /locus_tag="ACHARI_001323"
     CDS             complement(27331..27573)
                     /locus_tag="ACHARI_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442378.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            27694..27766
                     /locus_tag="ACHARI_001324"
     tRNA            27694..27766
                     /locus_tag="ACHARI_001324"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27727..27729,aa:Thr,seq:cgt)
     gene            complement(27885..28523)
                     /locus_tag="ACHARI_001325"
     CDS             complement(27885..28523)
                     /locus_tag="ACHARI_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8442379.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         9721 a         5075 c         5817 g         8008 t
ORIGIN      
        1 gtaaaaaagg ttgattcctc atagattaag ctttaagctt ggactgtaag aatctgatag
       61 aaaagcagga gaaaaagatg tatccacaca cgaaacagct tattttgaaa aagatggaag
      121 aaggatttta tccaggtgta gcagtcgcct tcataaaaga aaatcaagta gaaacattta
      181 cagccggctt tgcccaagtc gttcctgaaa agcagctgat gacagaagat ctgttgtttg
      241 atgcagcctc actgacgaaa gtcgttgcaa ccacttcatt agttttacgt ctattagaag
      301 atggaaagat agaactcgat caaccgcttc ataaatatct tcctgaattt gaaaatcaga
      361 caatcacttt gcgtcattta ctgacacata cttctgatat caaaacatgg ataccaaaca
      421 gagataagct atctcaggaa gaattgatcg ccgcttattt gaagctagaa ccaggtgagc
      481 aactaggaaa agaagtaaaa tatacagatg ccggtacgat tcttcttggg ttgatgctag
      541 aggaaattta ccaagaaact gcagtggaaa tctttcaaga acaagtattg gaaccattag
      601 gaatgatgaa cagcttattt cttccttttc cttccaaaaa aatcgttccc acagaacaac
      661 tgccttcggg tgaggttttg cgtggacaaa ctcatgatcc gaaagcaaga gttttggctg
      721 aacatgcagg taacgcgggt ctctttattg atttgaaaga cagcattact tttgtccaaa
      781 tgtatttgaa caagggaaaa atccgtaatg gtcgtttttt agaagaagag acgattgatt
      841 ctttactgca agatcaaaca ccgaaaaaaa atggctatcg ttctttagga tgggacttaa
      901 aaaaggatgt agaagacggt catgcgattc tttttcatac tggttataca ggtacgtttt
      961 tcttgattga tataaaaaca caagaagcgt tgatattttt gtcaaatcgc gtacatcctg
     1021 tcgatcatcg tgcggagtat ctcatatgtc gagatgaaat tgttgaaaac tttttgaaag
     1081 agaaagcggt attaccgaaa taatgctata atagctatga aaaatgagag gagtttgaat
     1141 tatgcaggaa cctatgtttt taaaaccagt ttttcaagaa aaaatttggg gaggaagtcg
     1201 tttacgttca gtctttggtt ttgatatccc taacgataaa atcggagaag attgggcgat
     1261 cagtgcgcat cctcatggtg tcagtgtagt agaaaatggc gaattcaaag ggaaaagatt
     1321 agatgaacta tggtcagaac acaaggaatt gtttggccat ccttcagaac cagtgtttcc
     1381 attattgatc aagatcttag acgcagaaga tgatctatct gtacaagtac atccagatga
     1441 tgcctatggg atgaaacatg aaggtgaatt aggaaaaact gaatgctggt atatcatcga
     1501 tgctgaacct ggtgcagaaa tcatctatgg tcatcatgca aagacaagag aagaactagc
     1561 tgagatgatc aaagatgggc gctgggatga ccttttgaag aaagtaccag ttaaaaaagg
     1621 tgatttcttt tatgtaccaa gcggaacgat ccatgctatt ggtaaaggaa tcatgatttt
     1681 agaaacacag caaagttctg acacgactta ccgcgtgtat gactacgaca gaaaagatgc
     1741 gcaaggacaa acaagagagc tgcatatcca acaatcgatc gatgtaacaa ctgtccctgc
     1801 gaaaacacca gaattacaaa tcaaagaagt ccgaaaaggg aattcatcga tcgtcactta
     1861 tttagaaact gagtttttca atgtatatga gtgggatatt aaaggaatca catctttcaa
     1921 aaaacaagcc ccttatacat tgatgacggt aatcgaagga gcaggagacc ttgtcgttga
     1981 cggaaaaaca tatccattag aaaaaggaac aagctgtatt attccaaatg gcgtttctga
     2041 gtgggcggtc caaggtgaac tagcaatcat tgcgtctgaa ccaggcaaat agttcccaga
     2101 tagctgaaag cagcagacgg tttttcgttt gctgcttttt ctacataaaa tccgtccatc
     2161 aataaataga agtctggtga aaaagatgga atacaaatgg aaagcaaaca acagcagtcg
     2221 cgtgtctatg aaacaactga gaaaagctgg acatcactta aatgaaatga tcatgccaaa
     2281 tctaagtctg tttatcgctt ggggaatctt gtcattagca acaaaaggtg tgtctggcga
     2341 tttgcacacc acattagaag aggtatgcca ttggatgatc cagctgcttt tacccatctt
     2401 gatcagttat tcaggcggga aatcggtagg gggacaaaaa ggggcagtcg ccggtgcaat
     2461 tgcttcttta gggttgatcg ctcgttccaa tgctccacaa atcgtaggag cgatgattat
     2521 cgggccgctt gccggcatcg tatatgaaca atttgtttct agattccatg tgaaattcaa
     2581 ggctggatat gaaatgcttg tcagcaacct actagtagga ctgactggta gcgttatttg
     2641 tataattgga atcgttttta ttgaaccagt tttaaatagt gttcacttgt tgctcgcctc
     2701 cgttgtgtct tggctagtca caatgaatct gttaccactt gtcagtctga tcttagaacc
     2761 tctaaaagta ctatttttca ataatgcaat caatcatggt gtattgacac ctttaggaat
     2821 cgaattctca caaacagtcg ggtactcttc cttatttttg atggaggcaa atcctggacc
     2881 tggtttaggg atcttacttt ctatcctttg ttttgcagaa aaacaggaaa aagtaaatgc
     2941 gagtggagca ttgatgatcc atgcgattgg cgggattcac gaaatctatt ttccatttgt
     3001 cttgctgcga ccgtatctat ttttagctgt aatggctggt ggggcaagcg gcacgttgat
     3061 atttcaatgg ttagatacgg gattagctgc acctgtatcg cctgggtcac tactcgtgat
     3121 ttttgccaat gcgccttatc cacaactagc tggggtaagt atagggatag caatcagtac
     3181 atttgtgacc ttcatttgtg cagcgatact attgaaatta ccaaataaag aaaaagagaa
     3241 caaaacggtc aaagaaaggg gagaaatctt ggaaccatct atcattcatt ccgtcatttt
     3301 tgcttgcgac tcaggtatgg gatcaagtgc tatgggagcc gccctgctga aaaagaaatt
     3361 agcagcagaa aaaatcgatc tttctgtcgg atacagctct atctatgaat tgaataacca
     3421 agtaaatcag ctggttatcg tgcaacatga actagcgggt cttgcaagag aaaaagtacc
     3481 tcaaagtcaa atctatgtac tggatcactt tttggaaaca gatatcttgc tgtcacgaat
     3541 aaaagaagcg atccaatttc caaagcggga agaaaaaaaa gagatagcag atatcacaga
     3601 acaaaaaacg tcgcctgcta atattgtatt tttgtataaa aacaacagac gcggctcaca
     3661 aactatgggc atgacgattt taagtcaatt agcaaaaaaa gcgaaaaaac agtttgttat
     3721 ctcaaaagaa tcaatagaaa aaatcaaaat ccaagaagaa acgatttata tcgcacaaag
     3781 agaactagct gtaactgaac agctagaaga aaagatccct cggctagttc tagttgaaca
     3841 ttgggtagct acgaatgaat acgaaaaact cgtgaaggga gaaatataag atgtatttat
     3901 cgcccagaga aaaacaatta ttagcagaat ttctcagtag cccgtcacct gtatctatcc
     3961 aaaaaatgat gaatttattg aaggtcagta aacggactat ttaccgcgaa ttagataatc
     4021 ttgaagtctc attgaaatcg gtaaatgctt ctttgaaaaa agcagctaga ggctcatttg
     4081 tcattgaggc atcagaagaa gtattagagc aattaaaaga aaatacagat gggaaaagta
     4141 atgacttatc cactgctaaa cgacaacacg cgattttagc cgagttgtta ctgacgacag
     4201 agccgttgag tcttgctcat ttcatggaat catatcgcat cagtaatacc actttttatg
     4261 cagatatcaa acagttggaa gaaagtattc gccaactgcc tttggaaatc attcggaacc
     4321 agggatacga gatcgaagga ccggaaaaat accgccggct tctgacagcg aatgtattag
     4381 aattagaaat caatgaatat gaactttttc atagtatttc tttcgatgca tcactgaatt
     4441 atttttttcg attcgttgat ccgcaacatc tatctcttgc acgcaaagta atcggggaag
     4501 aattgatcca aaaaaagacc aatctaagtg atagaaagct tgaacatttg gttttgatgc
     4561 tgacattgac gatggagcgt gtgacgaaaa atcatttact gacaaatgag acatataata
     4621 gtctggcaaa caaagaatta ctacagacag ctaaacgatt gttttcacgg atcgcttctg
     4681 agacaaaaca actttatcca gtaaatgaga tcgtcttttt tgcaggatta ttaaatgact
     4741 tcacgaactc atttgatcag aatttctttg aagagacgtt cgatacacag cttgcttatt
     4801 tagtaaaaca actgattcaa gaagtaagtg aagagacgaa tgtccatttt tatgaagatg
     4861 aaactttgta caaaatgctg ttgactcatt tatctggtgt gttttcaaga gccgttttac
     4921 aagaagagac actgaccaat ccgattttag aaaaaatcat ggttcaatac gaggaagttg
     4981 cgaatgcaat ccgtaaagca gatttttcaa gaaaaaaatt atccgaagaa gagatcgctt
     5041 atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc aatatcagta
     5101 ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta cgattgaaaa
     5161 ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta aaaaaaatcg
     5221 atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga tacggtggaa
     5281 aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta aaagaagctt
     5341 tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat aacgagtgga
     5401 tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat caattactcg
     5461 gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg attcgcagct
     5521 tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat cgccggctgc
     5581 ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg ttattccata
     5641 catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa catccgttga
     5701 ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt atgctggcgc
     5761 cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga gcaatcatca
     5821 tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt tatcagttgt
     5881 tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat acagctacca
     5941 gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt gaccgagatc
     6001 ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc aatgatcgaa
     6061 agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca tgggactgac
     6121 aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga tggtgtggac
     6181 tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc tttaaaaaaa
     6241 gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat cgaccaagta
     6301 atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga acatggacat
     6361 atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac ttcaaccaag
     6421 ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat gcaagctttt
     6481 gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac aaggtttttg
     6541 tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata gtgcccatac
     6601 ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc atgaaagtaa
     6661 cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt tgaaaatcaa
     6721 cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa gaaaacaagc
     6781 gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga gaatgttcaa
     6841 ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac aacaggaaca
     6901 gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga ccaatgatcg
     6961 ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt atagagacga
     7021 tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta aaacggatgc
     7081 tatgtttctt taccatacta tccagtaata gattgactag agagattacc tgctttcttt
     7141 tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc aaaaaactag
     7201 aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc gctggcaagt
     7261 caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac tcgacacgtt
     7321 tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga tccagtatat
     7381 ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg ttttttgcat
     7441 gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat tctttctcat
     7501 tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta tgattcagtt
     7561 catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca aagagcacga
     7621 ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg cgttcaaatg
     7681 taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa tctcttccat
     7741 atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag aacaaatcga
     7801 ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt tctgccattg
     7861 tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca acggttcatc
     7921 gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat gttgtttcat
     7981 tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca atcgttggaa
     8041 aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca aataatcaac
     8101 agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag accggcgata
     8161 atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag ttgttggcag
     8221 cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg caatcaaagc
     8281 agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt ttttaccacg
     8341 tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat aaaatttaat
     8401 aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg tttgcatgtt
     8461 ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac cgaaatcttt
     8521 ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt acgactattt
     8581 ctctttttag taagcggata caattagagt atcaaaagga gagagggaga gacaaaatgg
     8641 aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa ttaggcagta
     8701 ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt gtattaacgg
     8761 ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg gtaagcccaa
     8821 tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta attgctggtg
     8881 accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga acagatattc
     8941 caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc aagaaatttg
     9001 atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat aatttttcag
     9061 caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt ccagttgtcg
     9121 atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc catttgattc
     9181 cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat gcaatcaatc
     9241 atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa tctattctat
     9301 tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt actttgtttg
     9361 gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt ttaggcggga
     9421 tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta tcagttatag
     9481 gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta agagcgccag
     9541 cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct taccttcctg
     9601 ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca gtaattttga
     9661 aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag acaaaggcag
     9721 ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc tctggcgtac
     9781 aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct
     9841 tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc
     9901 aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa gaaagagcaa
     9961 aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat tcacctcgat
    10021 atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct gaaattgaga
    10081 atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag attctacttg
    10141 tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa gatatattag
    10201 acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta aaagatgaac
    10261 ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg gctgcacctc
    10321 aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa tcgattgcac
    10381 aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc cattcgacat
    10441 gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt gcggtcagat
    10501 gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag atagagacaa
    10561 aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag atgaagcaaa
    10621 aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg tagattttgg
    10681 taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag tcggagatga
    10741 gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca atgttgtcca
    10801 gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg cttaaggaga
    10861 gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat ttattggaga
    10921 ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca cgatcattga
    10981 tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa aagaaaaaat
    11041 cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga
    11101 aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac cttttattgc
    11161 tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa catctttaga
    11221 tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag aagtgtataa
    11281 atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg cagcagtcga
    11341 tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg aacctttcaa
    11401 agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa aaggggtttt
    11461 atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca atactggtca
    11521 tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg aagcaatgca
    11581 agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa gtttgctgat
    11641 tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa tcattcatcg
    11701 cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac cattacgtaa
    11761 gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac ataacttgac
    11821 ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc ctgaagatga
    11881 acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat tgattcaaga
    11941 agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta
    12001 tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa atttttgtca
    12061 cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag atccaattct
    12121 ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt ctcatttctt
    12181 gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac accttcagat
    12241 tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg ttcaccttca
    12301 cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt gaattcttcg
    12361 ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt gtagtgatcc
    12421 agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg acctagcaat
    12481 tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt gatcaaagaa
    12541 tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg accaccgcca
    12601 aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac gatctccgta
    12661 tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt tccgttatca
    12721 ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata attagccacg
    12781 gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct gcaatacagc
    12841 agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt tttcgattac
    12901 ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa cttttagacg
    12961 taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat ccatatcggc
    13021 aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg cgtaattggc
    13081 atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat cgattacttg
    13141 attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat tgatattgat
    13201 gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga ttttatcagg
    13261 atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga cattatacat
    13321 accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg acacattgtc
    13381 catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt cacaatttgg
    13441 acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga tgtgatgaca
    13501 atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat cttcttcatg
    13561 gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga ttggtgaatc
    13621 accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa tcattttata
    13681 ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg taaataagaa
    13741 cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta tttctaggac
    13801 agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata caattccctg
    13861 taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc gcaaaatatt
    13921 gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga cgacttggta
    13981 gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt tacctggtaa
    14041 acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc caactgcaat
    14101 cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct gacgaataaa
    14161 ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta ttcttaagaa
    14221 aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac aaaaaaacag
    14281 cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct tacctatatt
    14341 cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat ctcgtgtttc
    14401 ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa acatcagtgt
    14461 agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag ataaaccagt
    14521 tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt cttctgtagc
    14581 agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga caacgactga
    14641 ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat cttcactttt
    14701 gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa tcccgccgcc
    14761 tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca gtgttgtgat
    14821 ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa tcgaataaaa
    14881 catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt ccttttccaa
    14941 tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca cagctaattt
    15001 ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata atagccattc
    15061 ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa acatgatcag
    15121 gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga cgtcaaaccc
    15181 agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat cacgccaacg
    15241 atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa gtaattgaaa
    15301 ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca aacgcggttg
    15361 attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt tagaaaaaat
    15421 gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata atatttccag
    15481 cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata gtccagtcac
    15541 actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc tatctggttc
    15601 gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata tataataaaa
    15661 aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc gccgctgcag
    15721 ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt cgtttctacc
    15781 agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac aatacagctt
    15841 tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa ggaggaggaa
    15901 taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa aaataaatta
    15961 tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa agcctaagat
    16021 attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca tgtctcaata
    16081 tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg agtatttgcg
    16141 aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg accatcatgt
    16201 tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt taacaaacca
    16261 tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata tgcgattaga
    16321 tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg ccctgaccat
    16381 ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc aaaagattta
    16441 cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac gctctttgca
    16501 aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt tcgctgccaa
    16561 gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact taaaaacatc
    16621 aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga aaaaaataaa
    16681 gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt aaggaaacaa
    16741 gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt ttgaagccaa
    16801 tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg aatagttcca
    16861 gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca actacttcag
    16921 tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg acaagtacat
    16981 gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac agctcaagcg
    17041 aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca agtacaagtc
    17101 cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat gataacacgc
    17161 aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat acccaaacgt
    17221 ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca acggggtgca
    17281 taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat atacgatttt
    17341 aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc aaaaccaaaa
    17401 ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt ttactgtttt
    17461 aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc agctttttgt
    17521 gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag ataatggaaa
    17581 ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag attatcaatt
    17641 gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa caaatgaact
    17701 cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt actatgtcaa
    17761 gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg acatggaaaa
    17821 agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg gattgttcat
    17881 cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc gtctgaaatt
    17941 gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta gccaaagaag
    18001 aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa acgtttattc
    18061 ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa cgagaaagat
    18121 aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa taaagaaagt
    18181 acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt aggaggtaag
    18241 ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg gctattttat
    18301 tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg gaccctacat
    18361 taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga atggatatta
    18421 ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc atcggtatgc
    18481 ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa aagtatgatg
    18541 aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa ggagaatatg
    18601 cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg agtgactttg
    18661 aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg atttcaaaag
    18721 acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt gttttccgtt
    18781 attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag ctgtgacatg
    18841 ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt tgctcacttt
    18901 ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag cgtttttgtt
    18961 cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa agcccgtttt
    19021 ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg gatgactaat
    19081 ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta aaatttcata
    19141 aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg atagaaggat
    19201 gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg ttcggacatt
    19261 tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt ctgaagcatc
    19321 ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc cagttccgcc
    19381 tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag ttagttcttc
    19441 gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag cgatggaatc
    19501 aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt taacttatta
    19561 tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga atctagaaag
    19621 aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt ccgagaaata
    19681 ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata tatcgaggaa
    19741 tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg ctttatcact
    19801 ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga ataaaaaaag
    19861 actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt tacgcagcaa
    19921 aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct atatatgatc
    19981 aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt ccaaatttat
    20041 gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag tgagcaatag
    20101 atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg aaaatcaagt
    20161 ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta ttttttaagt
    20221 atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat aattatgaag
    20281 aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat ggtttaggcg
    20341 ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa gaaacagacg
    20401 tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc gatgaagcgc
    20461 aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa ttggatattc
    20521 cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa ggatcaaaat
    20581 atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg ttctgccaca
    20641 aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa ggcgatcaag
    20701 tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac tttcacccat
    20761 caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg aagaccgtta
    20821 tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa agcgttttat
    20881 gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc gtcgttataa
    20941 agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac tggatgatga
    21001 aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag aagtcttaga
    21061 agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa atatcatcat
    21121 ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg atcttttcaa
    21181 tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg aagctaacgt
    21241 taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca acgtatattc
    21301 gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa ctgaatcgca
    21361 agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg aagaagtaga
    21421 gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag gtgctggcgg
    21481 acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa cagggatcgt
    21541 tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa tgaaaatctt
    21601 gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg atgccaatcg
    21661 aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata atttcccgca
    21721 aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc agattttagc
    21781 agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat caaaactgga
    21841 agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct tcttcttttt
    21901 tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa cgaaaacaat
    21961 ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa gaacagcggt
    22021 tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat ttgctaggat
    22081 atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc cctcgaccag
    22141 aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg ttagaagtga
    22201 tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca agaaaaaatt
    22261 ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa gaaaatgctc
    22321 agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca gtaatggatc
    22381 agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa tgggaactga
    22441 tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag aacgatggat
    22501 tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca aatggaaaga
    22561 tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt cagcaagcat
    22621 ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga atgatccaag
    22681 tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat aaaaaaacac
    22741 ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc actattgaca
    22801 aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa taagaatgta
    22861 tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac agaattgcaa
    22921 gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga aacagtatac
    22981 ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc tgtaaaaggg
    23041 agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt aaaagagtat
    23101 gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc tggaccatta
    23161 actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg aggattatcc
    23221 acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga actttccggt
    23281 gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac gactgcagat
    23341 catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg agctacgcga
    23401 attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc tatgatttta
    23461 cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc tgttgctatt
    23521 gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa atacaaacac
    23581 tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac tgcagttcaa
    23641 tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat tgcggatcaa
    23701 gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga agcggcggct
    23761 aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt agatttgatt
    23821 tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg cttgaaaaaa
    23881 gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat tcttttagta
    23941 tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc gtaactaatc
    24001 gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat aaaattgtag
    24061 gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc tgatctttgg
    24121 gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat tgcctctgaa
    24181 aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa taaatacgca
    24241 gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat cgttgaaaat
    24301 ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt ccagcctcac
    24361 tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg ggacacgatc
    24421 ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt caacttcagt
    24481 ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt tattgattat
    24541 aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc aggtgccagt
    24601 gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga agtaggcgca
    24661 aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact tcatcctaat
    24721 ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg tggccctcga
    24781 ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag tgcggtattt
    24841 cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt agcattcaaa
    24901 gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa tgcaaaagca
    24961 atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc gacagataat
    25021 catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc agagagcatt
    25081 ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac actaagtcca
    25141 ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg gttcaaagaa
    25201 gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa agatgataac
    25261 gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt tccgttgcat
    25321 aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg gcttttctgt
    25381 ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag tatatgaggt
    25441 gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat cgtatctcgt
    25501 tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt atctatgaac
    25561 tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct gtgaacaaat
    25621 attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa ctcaatattc
    25681 gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat attcatggag
    25741 caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa cttgcggttc
    25801 gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca aaatatccaa
    25861 cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc aagtcaaaaa
    25921 atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac atggctacag
    25981 tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa ttgctgtatt
    26041 atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga gaaaactatt
    26101 ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat gaagaagagc
    26161 gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat gatcttcctt
    26221 cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag gcttatgcgc
    26281 gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga atcatccatg
    26341 attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca atggcattat
    26401 ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa acttcttttc
    26461 aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt gattttgttt
    26521 tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa atttcaagtg
    26581 atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa ctgcggaaca
    26641 aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga agtatcacgg
    26701 gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac acaaaaaacg
    26761 ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg aatggttgac
    26821 ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta tcgagatgaa
    26881 gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga tgcacgtcag
    26941 ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc aatcgatgca
    27001 ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc acctgaggga
    27061 gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc tctagatgaa
    27121 aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga ccgtttgttt
    27181 ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca tccttttttg
    27241 gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc atataaaaaa
    27301 accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt tgtagttaac
    27361 gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt catcaatctc
    27421 aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact tgccgtgaat
    27481 tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg gtacgatagt
    27541 tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca tcaattatac
    27601 cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc tttacaatca
    27661 gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt ggtagagcaa
    27721 cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa aagagaagac
    27781 agatatatca aggattttta atccattgat acatctgtct ttttgtttaa aggaaataaa
    27841 gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga gaataaaacg
    27901 actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt aatgatcgag
    27961 ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat caaaaggatg
    28021 tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat aaatccaaag
    28081 atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa aatagcgaga
    28141 aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat aaataataac
    28201 gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat ttgttgcaaa
    28261 aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc tatatttttg
    28321 acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa aacaagtgta
    28381 aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg cgcaaatagc
    28441 aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt aagttctatt
    28501 ttttttccta aaatgttatt catgataata actcctacta ttcagttttt gaagatagag
    28561 tcagtataca tgaagagtta aaataggcaa tgttttttta tggatatttg tattgctaaa
    28621 a
//
LOCUS       DAYYAO010000033        26399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000033 DAYYAO010000000
VERSION     DAYYAO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.035"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..429
                     /locus_tag="ACHARI_001326"
     CDS             133..429
                     /locus_tag="ACHARI_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8442380.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1073..2026)
                     /locus_tag="ACHARI_001327"
     CDS             complement(1073..2026)
                     /locus_tag="ACHARI_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8442381.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2049..2804)
                     /locus_tag="ACHARI_001328"
     CDS             complement(2049..2804)
                     /locus_tag="ACHARI_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442382.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2801..3760)
                     /locus_tag="ACHARI_001329"
     CDS             complement(2801..3760)
                     /locus_tag="ACHARI_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8442383.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3753..4703)
                     /locus_tag="ACHARI_001330"
     CDS             complement(3753..4703)
                     /locus_tag="ACHARI_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8442384.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4853..5305
                     /locus_tag="ACHARI_001331"
     CDS             4853..5305
                     /locus_tag="ACHARI_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8442385.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5394..7661)
                     /gene="gshAB"
                     /locus_tag="ACHARI_001332"
     CDS             complement(5394..7661)
                     /gene="gshAB"
                     /locus_tag="ACHARI_001332"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8442386.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7848..8846)
                     /gene="ftsY"
                     /locus_tag="ACHARI_001333"
     CDS             complement(7848..8846)
                     /gene="ftsY"
                     /locus_tag="ACHARI_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8442387.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8857..9666)
                     /locus_tag="ACHARI_001334"
     CDS             complement(8857..9666)
                     /locus_tag="ACHARI_001334"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8442388.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYGQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9675..13256)
                     /gene="smc"
                     /locus_tag="ACHARI_001335"
     CDS             complement(9675..13256)
                     /gene="smc"
                     /locus_tag="ACHARI_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8442389.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13276..13962)
                     /gene="rnc"
                     /locus_tag="ACHARI_001336"
     CDS             complement(13276..13962)
                     /gene="rnc"
                     /locus_tag="ACHARI_001336"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8442390.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14310..16091)
                     /locus_tag="ACHARI_001337"
     CDS             complement(14310..16091)
                     /locus_tag="ACHARI_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8442391.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16117..17031)
                     /locus_tag="ACHARI_001338"
     CDS             complement(16117..17031)
                     /locus_tag="ACHARI_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442392.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(17058..18020)
                     /gene="opp4B"
                     /locus_tag="ACHARI_001339"
     CDS             complement(17058..18020)
                     /gene="opp4B"
                     /locus_tag="ACHARI_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8442393.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(18020..18964)
                     /locus_tag="ACHARI_001340"
     CDS             complement(18020..18964)
                     /locus_tag="ACHARI_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442394.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18965..19969)
                     /locus_tag="ACHARI_001341"
     CDS             complement(18965..19969)
                     /locus_tag="ACHARI_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442395.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20237..20479)
                     /gene="acpP"
                     /locus_tag="ACHARI_001342"
     CDS             complement(20237..20479)
                     /gene="acpP"
                     /locus_tag="ACHARI_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8442396.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20601..21602)
                     /gene="plsX"
                     /locus_tag="ACHARI_001343"
     CDS             complement(20601..21602)
                     /gene="plsX"
                     /locus_tag="ACHARI_001343"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8442397.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(21651..23687)
                     /gene="recG"
                     /locus_tag="ACHARI_001344"
     CDS             complement(21651..23687)
                     /gene="recG"
                     /locus_tag="ACHARI_001344"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8442398.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23804..25486)
                     /locus_tag="ACHARI_001345"
     CDS             complement(23804..25486)
                     /locus_tag="ACHARI_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8442399.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(25538..25900)
                     /locus_tag="ACHARI_001346"
     CDS             complement(25538..25900)
                     /locus_tag="ACHARI_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8442400.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            26170..26358
                     /gene="rpmB"
                     /locus_tag="ACHARI_001347"
     CDS             26170..26358
                     /gene="rpmB"
                     /locus_tag="ACHARI_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8442401.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7288 a         5732 c         4658 g         8721 t
ORIGIN      
        1 acaaaaaata ctataaaaaa taaaaaacct ttaacaacta ttggaaaaag aattactttt
       61 tgttcacgga ttattaacaa ctcttcggta gagtaaactt atcaaataaa agacttcttt
      121 ggaggaatca ctatgaagga atatataaaa aacatctatt ttatcgaaga aacacaaaat
      181 atcgagggtt cttatattga agtaaaaaca ctctttgtca atgaagataa aaccaaagca
      241 ttggatattt ataaaaaatt agcttcaaaa aaaacgaata gttttggatt gatattaagc
      301 gagtataaaa tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag
      361 cttcccgccg atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca
      421 catgcctaaa tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt
      481 gaacattgtt tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg
      541 acagaaaata gcttctcata ttttctagtc tagtgggctt attgccgaga agcagcagat
      601 ggaacatcgt ttagaagaga ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg
      661 gacagaaaat agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga
      721 tggaacatcg tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat
      781 ctaagtttcg ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa
      841 tggatcacac tattttttgc cactccccat tctagaatca acccgtatga tgtagataat
      901 gaattcacgt tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata
      961 cgattttacg gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg
     1021 taagaaacat tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa
     1081 atgcttgatt cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat
     1141 accagacatc tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta
     1201 ccagttcatt ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt
     1261 ctactacaaa taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac
     1321 cgtgagtgga tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga
     1381 aaccgaatct agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta
     1441 gtgctttttt gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt
     1501 cctctaattg agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat
     1561 tttgctggaa agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa
     1621 tttgacttag atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct
     1681 gaagctgatt aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt
     1741 tataggatga aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa
     1801 caccaagagc atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat
     1861 tatttggcac ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt
     1921 gtgtgctgct tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta
     1981 aaccaaatga tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa
     2041 tgttagtttt agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc
     2101 atttcgtata attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta
     2161 aataactgtc catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata
     2221 tcatgaagaa caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc
     2281 atgatttgaa ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa
     2341 tccgtgtctt gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga
     2401 gtgtcaatcg attgatccgc tatttcttct aacccaaggt gttccagtga ttcctgaacg
     2461 atttcctcgt cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact
     2521 aattctttca cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt
     2581 ttggctaatt cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct
     2641 gtatcttttg gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt
     2701 ggaccgataa aagcagtcaa cttcttttca ggaatcgtta actgtacatc tttaaccact
     2761 gattttccat ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt
     2821 cgcgaatcaa ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg
     2881 ttgtattcca gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag
     2941 ctaccagaaa gcttccgata aatagctgac catgacgata agtagaaaag acacgataac
     3001 tgatatttgc gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc
     3061 ctgtcaaaag actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga
     3121 ggtttactgc ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata
     3181 acaggtacca gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat
     3241 tgccaaagct tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga
     3301 cttgtaaaaa ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta
     3361 ataaaaaaag attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc
     3421 cgaccatcag cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca
     3481 ctcctccgat aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca
     3541 atactgaagg agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc
     3601 tgacagctag cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa
     3661 aatgccagtt tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa
     3721 caagcatacc aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga
     3781 agataaagga agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa
     3841 ggaaaaatca acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa
     3901 gcagttacgc caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga
     3961 atgatcactc cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat
     4021 gatactaagc caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca
     4081 aacgattcgc ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac
     4141 aatggaacaa cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct
     4201 tgaagccatg aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact
     4261 gaaccaatga tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg
     4321 aggaacaatc gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct
     4381 gtcaaggatc ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct
     4441 aaacgcgcac tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa
     4501 tgttgcatga tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt
     4561 cgtggcaaac gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct
     4621 gacaattgta cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat
     4681 gctactagtc gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct
     4741 tattttaagt gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg
     4801 ggaatatgct ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca
     4861 agatgatgta aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga
     4921 agaacagcat aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc
     4981 tgaaatgatc caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga
     5041 tgcttccata ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct
     5101 agcaacgaaa aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga
     5161 ggaattcgga ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga
     5221 aaaaaaatac ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa
     5281 aagcttctgg gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa
     5341 agcttttgat cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat
     5401 acttcaggat ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaaa
     5461 ggataaacat gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt
     5521 ttatcagttg taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta
     5581 gctcctagtg cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca
     5641 gtcatatcga tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt
     5701 tgattggctt ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct
     5761 aattcaccta attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca
     5821 ctattttttt cttcaacaag ttctttcact gttcggatac tgtcaccaat aacattagca
     5881 gggactcgca acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt
     5941 cccggcataa attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct
     6001 gcttgataat cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg
     6061 gttgatttag gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct
     6121 tcttccatgg atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact
     6181 tttttcgtta ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc
     6241 atgtttgcat ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc
     6301 gactcatcta atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga
     6361 gtagaaagtt ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta
     6421 taatattgat gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga
     6481 tagagtttcg ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact
     6541 aaataaagag aatccaacca atccaattca accagcattt gcttcattcc ttcaagaatt
     6601 tctctacctt cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta
     6661 tttcgagtcg taccttctgc tacccattgg tcacagtctt ctttttcttc tgtccataac
     6721 atgtacatca ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca
     6781 taaggattca aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct
     6841 gcgactttct ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt
     6901 ttcccttctt cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa
     6961 actttcacat ttggatgatt cgtatagccc agcgcactgt tgcgaatact gcgaacaggt
     7021 tcttgcggat gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat
     7081 aaataagtga tcatccagcg atagcgcata aagtttctcg ctgttttcaa ataaagttct
     7141 gttttgaatt cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac
     7201 tcatcgccaa attcaaaatt gaaatgaata ccgcttacca tctgttttcg cttcccgtat
     7261 ttttttgcta aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg
     7321 atctcttcgt ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct
     7381 ttcggtatag aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga
     7441 atagaatcgg taacaggggt gatcatctct gtttgtgttt cgctaaaatc tgtttggata
     7501 taggggtgat aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct
     7561 gcaagatcaa cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg
     7621 cgcgcattga catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat
     7681 atgaatctaa ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt
     7741 acaaataagt ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa
     7801 acaaggagct gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt
     7861 agtaatcctt tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg
     7921 attccttcac caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt
     7981 acgataccgc cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt
     8041 gtttctttga attgttttgc ttgaaccatc gcgttttgac cagtggttgc atctaataca
     8101 agaagtgttt cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct
     8161 aattcgttca tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca
     8221 tctgcctgct ctgtctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgcct
     8281 cctgcattgc cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg
     8341 attgctccag cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg
     8401 aactgatggg caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac
     8461 aaaataactg ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc
     8521 gcttcataga gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc
     8581 gcatttctga gtttcacttc ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa
     8641 cctacatctg caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt
     8701 cgaaaattgg caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa
     8761 cccttgtcgt acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca
     8821 cgcttgattt tatcaaaaaa tcccatttga ctccccctac tctaatacaa atttttggat
     8881 cactttggct acgccatttt cttcattgct ggctgtcacg aaatctgctg cttctttgac
     8941 gaaaggcaca gcattctcca tcgctacgcc tagtccagca tattggatca tcggtaaatc
     9001 attttcctca tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat cttttgccaa
     9061 taaagaaata ccataagctt ttgtaacacc ttttggcata aattccaaaa gcatacttct
     9121 tgttttgatg atctcataca tttcatggaa ttctgctggt atttctttga ttttaggatc
     9181 taattcttct tggccataag cgattactgc cttattaagc acaaattcat ctgtaatatc
     9241 gcttatatcc gctggttgga attgcagcag cttattcaac acattgtaca aggattgctt
     9301 ccttggggca ctaggcagct gtaaaaccac attgtcagac aaaacatcta gtggcagatc
     9361 caattgctga gccagtttgt ataagcgttg gatatctgat acttccatca ctttcttttc
     9421 catgatttca ccagtatcat ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc
     9481 accttcatct accagtccta attcttgcag ataatgcgcc atcgcagcta aggggcgtcc
     9541 tgtacataga acgatcttga ccccttgctg ttttgcttgt gctaatgctt gtttattctc
     9601 atctgaaatc agcttttgtt catttaataa tgtaccgtcc aaatcgattg ctactaattt
     9661 gatcattttc gttcctacac tttcgtttcg ttgattttcg cctcttcatt gatatcttcc
     9721 atccgtacag aaacgatttt tgatacaccg gattcctgca tcgtcactcc gtacagtaca
     9781 tctgctgcga ccatcgttcc tttgcggtgt gtgactacga taaattgtgt gtcatcttgg
     9841 aaatcgctca agtaacgacc aaaacgtttc acgttggctt catctaacgc tgcttctacc
     9901 tcatctaaga tacaaaacgg aacaggacag acacggataa tggaaaagag caacgcgatg
     9961 gctgtaagtg ctcgttctcc tccagaaagc aaacttagac tttgcagttt tttgccagga
    10021 ggttgtactt caatctcaat acctgttttc agcaaatcag ttggttccgt cagtactaat
    10081 tcagctttac cgccgccaaa catatttgga aagacaattt tgaattcttg acggatagct
    10141 tcaaatactt ctttgaatct tgtccgcact tcatcatcca tctcatccat cgtttcaaac
    10201 aattggtttt tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt atgtctttca
    10261 ctgacttgtt cgtattgttc gattgaattt aagtttactg gtcccagttt ttcaatctgt
    10321 tcttttaagg atgaaacctt tgttctactg tcttcgatat cggttgtttc ttgatagtct
    10381 tgggaagctt tctcaaatgt cagctgatat tctgactgca gataggtcaa atgattatct
    10441 aaaatcattt cagctcggtc tttttggact tctaatttgg attgttccga cagtcgtgct
    10501 ttttgctcac gattacgctc tgccaagact gcttcaagct tatcgatctc cttttgcctt
    10561 ctatctcgct gctctttggc ttttgccaac tcggctttta atgtttctcg ctgagcagat
    10621 aattcgttaa tttgtttttc caaactttct tctgtgactt catgatcaga aaagtctgct
    10681 gtcaatgtcg ccaactgctt ttctatcgct tcttgacgtt cggttgcctc tgctttttgg
    10741 actcgggcac cgcggagctg gatctgcaga tgattaaact gctctttcaa gacagcaaga
    10801 tctgcttgtt cttgtgcttt ttgactttgt acttgtgctc ttctggcttc catctggtcg
    10861 ctttcttgac ttaacgattt gatttcttcg tcgatttttt gtcgttgcga ttcaagatct
    10921 gtttgtttgt ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt
    10981 tcacgtgtct caaaagttga gatttgcttt tctttttcaa aacgttcaag ttcatttatg
    11041 atgttttgta gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta
    11101 cgcaaaactt cttgttcttc ggctaaacga gctgtttctt gttgcagctc ctgaactttt
    11161 ttttcttgtg cttggagttg tttatctgct tcttcaaatt cactagtcaa ctgtttcaat
    11221 tcttgatttt gtacaaacaa actgccttgg tttcctcgtt tattcgctcc acctgtcatg
    11281 gatcctccgg cattcatcac atcaccttct aaggaaacca ctcgatattg atagcggatc
    11341 gtttgcgcga ttgcattggc actagttaag tctttggcta aaaggatcgt tcctagcagg
    11401 ttttgaacca cagtttggat ttgatcggga taactgactt gttcactggc aatccctata
    11461 aacccttcta cagctgctgc ttgtgtcaat atatgagccg gtaattgtct tggtttgatt
    11521 gtcgtcagtg gaaggaatgt tgctcttcct cctctttgct gtttcaaata cgtgatggct
    11581 tgccgagcat ctttttcatt ttcaacgatg acatgctggg ctgccccacc taaagctgct
    11641 tcgatagcta acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc tccgacaata
    11701 cccgaaagct gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag
    11761 ttttcttgga tttcttgaag gcttttctta cgagcacgta gctgctggac ttgattcatt
    11821 aattgataca ttttaggctg ttcttttgct aatgcttctt ggaacttttt cccgttttgt
    11881 tggatcattt ctaacttctc ttttgtttca gttaaagatg attttagctg cgcctgcttt
    11941 tgtgtcaatt cttctttttg taagatcaat cggtctactg atgcttctac ttccgattgc
    12001 ttggctaacg tctgcttgct ttttgctgtt tcttgtatat attgacgttc tagatacttc
    12061 agttcgtttc ctacagctgc ttgttcttgc atcaaatcaa catattgatc tcttaattct
    12121 gccaataatt ctttcgttga tttttggtat ttttctaatt cttgctgtgt ttttatgaga
    12181 tttgcttcag ctttttggat ctctgtttct ttttctgctg ctgctttcat caagctttct
    12241 tgaagctttt caaaatgttt aacctttttt tgtgcttctg ctaatgaagt ttgatattct
    12301 tgcgagcttt tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg tccttctgtc
    12361 tgcttcagtt tttccgaaag atctaataat acttgctgat ttttttcgat cagacgatcg
    12421 gcttgcgcat tttctttacg ctgcttcgcc aaaatggatt cttgttcttg gatcgactca
    12481 gatagtttgc ctaattctag attgaatttc gcaagctgtg cttgtttgtt gtcccaatct
    12541 ttttttgctg tcttgatttc agcaaccatc aaagagacat cggtttgagt cagtgtttct
    12601 tttagccgta aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt taactgttct
    12661 tccaattcgt ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc
    12721 tgttctgctt tctttttccg ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc
    12781 ccacgacggt cttccggttt actactgaaa atcgcttcta cttttccttg agaaatgatt
    12841 gagaacgatt cttttcctaa tccagaatct aagaacaatt cttggatatc ttttaatctg
    12901 cagctttgtt tattgatgaa aaactcgctt tccccagtcc gtcgataacg gcgggttaca
    12961 ctgatctcat tgtactccaa tggcagataa tgatcactat tgtctaaaat cactgtgact
    13021 tctgcaatat tcagctgctt acgggtatcg gaaccggcaa agatgatatc cggcattttt
    13081 ccgcctcgca gacttttagc agattgttct cctaataccc agcggatagc ctctgtaata
    13141 ttgctttttc cgctgccatt tggtccaacc actgctgtga cgctattttc aaaatcaatg
    13201 actgtcttgt ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct
    13261 ttccctcact tacttttaca gtgctgccaa tgcattttcc gccgcagctt gttctgctaa
    13321 ttttttcgat ttcccttgtc cagtgccaat caattgatca tctacataaa cttcgatcca
    13381 aaatactcgt tcatgagctg gaccttcttc attgatcaat cggtattcga ttgatacatc
    13441 acctgatttt tgcaagactt cttgtagttt tgttttatga tccatctcat gtgaaaaagc
    13501 acctgctttg actttaggaa aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc
    13561 ctggtcaaga tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg
    13621 cgtacgtcct cctgaattct cttctccctt gcccaacaaa acatattgat caaagtgaca
    13681 ttctttggca aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa
    13741 ttttccttct ggcaaatgag gatataaacg gaataaatac tgagaaacca tcagttctaa
    13801 aacggcatct cctaaaaatt caagtcgctc attatccgat aattgcagat tgcgatgctc
    13861 attcacatag gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat
    13921 gttatagttt tcttttagtt cttttgtcag ctggttgtcc attttccaca ttccttttat
    13981 ggatattttt tcgatacttc cttattatac tggttttttg tcaaatttga aatggttcgg
    14041 tcaaaaggta agtaaagctt taataatcct agtgattgac gagaagttgt gaaaaattcg
    14101 tttagtttcg agtatgaaga aaaaattttg tgaaaatggc tttccatatt tttgcataaa
    14161 tttgacttaa taccgagaaa ctggactttg aacaccgttt attggagaag tcatgaaatc
    14221 agcgccttga cctgaatatt agagaaataa acagaaaaat atcaaaaaac cgaaaaagga
    14281 catcgccctt tttcggtcat ttttagtgat tatgaacgtg aatctgctgt aacagaaaca
    14341 tttgcccatg ggtttggtac atcataagac caatcccagt ctttgacacg gtcgttgact
    14401 ggcaatactt cattacggaa caacgttgga atcgcaaatg cttcttcaaa tgcatattgt
    14461 tgccattttt gatatgcttc tttttggtac tcttcatcaa atgctttgct tgaactgata
    14521 tcgttgatca attttgtatt ttcttctgat tcataacgag tgtagttgaa cgcagcattt
    14581 ggtccccata gtccacttgg atttggatca gttcctgtat tccaagcagc ttggtaaaca
    14641 tcaatttcag gatcatcgtt ttctaattta tcatagaaag aattgaattc gatcagacga
    14701 cctgttgtgt attggacatc caaaccaatt tctttccatt gttggatata gtaatctgcc
    14761 aatggttgcg ctgtttcccc accggacatc gaagcaaagt tgattgttag tttatttcca
    14821 tctttgtctt ctcttaaacc atcgtcatcc acatctttgt agccagcatc atctaatagt
    14881 tttttcgctt tgtctagatc ttgtgtgaat cctttcatct catcatcatg gtatgactgg
    14941 aatactggag gaatcaatga agtcgcattg cttctcaaac ctgcatagaa acgttcacct
    15001 actgcattat tatctaatgc atagcccatt gcttgacgta atgatttatc agccattttt
    15061 gaatctggat tgtattccac actctttgta tctgcattcc atttacctaa cttgaatcct
    15121 agataagtat aagaagtttg ttcacggcct aagtttgtat agccagcaat ttctttatag
    15181 gtatcgtaag tatctgttgg catttggaaa atcatatcat ataatttaga gttcaatgct
    15241 tcagttgcac ttgccatcgg tacagatttc atcgtgatct ttttcaattt aggcgtacca
    15301 ttccagtagt gctcgtttgg caagtattct actgattctc ctgcaacgat attactcatg
    15361 taataagggc caaaagtcac tggattttta cgtacttgat cgcttgattc catatctttg
    15421 attgctacac cttctaacgt atgttttggt gctgcatatg accagatacc accacccgat
    15481 tgaagcatgc ttgggtttac ttctttatat tcgatttcaa ctgttttgtc gtctattttt
    15541 ttgattccgg agattgtgtc ggcacttcca tcatgatatt cttccatacc tacgatgtta
    15601 cggaaagtgt cgtcataacg gataccagtg taatctttat tcccaatgat ttcgtaaggg
    15661 aaaatcacat cgtccgccgt tacatcttca ccatctgacc atttgacgtt gtcttttact
    15721 ttgattgtcg cttttttcgt atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg
    15781 attctgaaat cgtcatcgtt tgcaaatagc ggttcatgag aaggagccat aaaagctgca
    15841 tcatatgaat cttggtaaaa ctcccactgg aacaatcctt taaactgact gtccgttgcg
    15901 accgcagctt ctaattcgcc gtcttcgatt gcatcatcat tattttttgt tgcaattgga
    15961 aaagttaatt cctctttgtt attgccgttg cctccagagt tgtttccaga ggtatctgta
    16021 cctccgcccc cgcatgctgc aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc
    16081 gttttcttca ttcctatttc ccccttcgct tctatactag cctaaccgtt ggcgcgcatc
    16141 tgcagaacgc ttgaatgctt gtccaacata attgatactc aacatcaaaa ctaaaataag
    16201 gattgatgcg ggtaaccaga tccattgccg attcactaag acttctccgt tacttgcata
    16261 accgatcaat gtacctaaac taggtacatt tgccggcaag ccgaaaccta agaaagtcaa
    16321 agttgtctcg ataccgatgt ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga
    16381 acttagattc ggcatgattt cgcggaacat gattttaaaa tcattggttc ccattgtttt
    16441 tgaggcactg acataatctc ttcttgcttc agataatgtc ttgcttcgga ataatcgggc
    16501 tttagctacc caataaaatg cactcatgat gaatataaag gaccagacat tgtatctgga
    16561 aataatcgat acgaagacga taatgatcag catgatcggt agaatcatga taaaatcaac
    16621 gatccgcatc aagatgttgt caatgatccc tccgtagtag ccagagatga tccctaatcc
    16681 aaccccgatg atcgaagtaa ggatcgtgat cgcaaatccg atcgtaacag agtttctagc
    16741 acctatgatc aattgtccta atacatcacg tccgccttca tctgctccca gaatgaactt
    16801 ttgtccttgt gtcgaaactg ttcctggtgc agcgtatttg tcgaagatac taacatacat
    16861 cactttgtct tgatcggtca gcatcgcacc gataaagatc actaataata aaaccactag
    16921 caaacctaat gaaaaaatag caattttatc ttttttaaat tctcgagcaa tcattcgtat
    16981 ccccatagga ggaatacttt cttgaactgc tgtattgttt ttttcactca tcgatccatt
    17041 ctcctttctg attggtttta ttgaatccga atacgcgggt caacgatact catgatgata
    17101 tctgaaagta atgtccccac tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc
    17161 acggtatagt cccgttggcc gatggaatca ataaatagtt ttccgactcc cggataacca
    17221 aaaatctttt cgataactac agaaccagca atcaacgaag tgatttcata ccccatttga
    17281 gaagccatcg gcaacgctgc atttcggaaa atatgtcgac tgaatacttt attcgtcggt
    17341 acccctttgg aacgggctgt tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt
    17401 cgcagatatt ggattgtgac ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga
    17461 atatatttca aacggtctat ccattgagtg ataaatccac cttgcaaacc tagtgtttgt
    17521 gatccacttg ttgggaacca gcctaaacga taaccaaaaa caaatagcat gatcaagccg
    17581 aagataaata aaggaacagc aaaactaaag aagttgtaga ttacgacaat cttatccaag
    17641 attgaatttt gataccggcc agcaagcatc cccagaggca cagcaatcaa ataagtaagg
    17701 acaactgtca agatcgatag atataaagtg ttgatgatcc gttcgccgat caagtcagca
    17761 acagagcgtt tgtataagaa actttgacca aaatcgcctt gcaaagcatt tccgatccaa
    17821 cggaagtact ggacatacca tgggtcattc aagccagctg cttgtcgcat ctgttcgatc
    17881 actgccggat cctgatttgg attgatcaaa ccggtaaatg gatcccccgg catcgcttgg
    17941 gctaataaga agatcagaac acttaaaata ataacttgcg ggatcatcaa taaaacacgt
    18001 cgcagaatcg ttttccacat tagcttgcac ctccatcttt caacgccact ttgtgtgtag
    18061 ttgtgatcgt acgcaaatcg tagactcgac cagtcgcatc atagtattct ttttggtttt
    18121 ggatatactc ttgttctact cttcgtcgat tttctttgtg gctttcccta ttttctacat
    18181 cgatttgcgg aatagctgaa agcagacgtt tcgtatagat atgtcgcggg tcattataaa
    18241 tgtcttctct tgtaccgatt tctacaaatc gacctttgta cataattgcg atattgtcgc
    18301 acatgtgttt tactacacct aaatcatgtg aaatgaataa atagctgagg ccgtattctt
    18361 gctggattcg tttcatgaaa ttcaatactt gcgcttgaac agatagatcc agagcagata
    18421 caggttcatc ggctacgatc agttttggat tggtagcaac agcacgggct actcctagac
    18481 gttgacgttg tccgcctgaa aattcatgag gatatttata tagcgcatct tccggcatcc
    18541 caacgatatc tagcagtcct tttacctttt tcttttcttc ttgatcgctt aaccgttcaa
    18601 aatttcggat gggttcagca atgatatcca acactcgctt tttaggattc aaactggaca
    18661 ttgaatcttg gaagatcatc tgcacatcct tgttataacc catttttttg cggttgcttc
    18721 gcttcgttac atcttttcct tcatacatga tttgtccggc tgttaccttt tctaatccca
    18781 caacggcttt tcccgtagta gattttccag agccagactc tcccactaaa ccatatgtct
    18841 ttcctttttc gataataaaa tccacaccat ctacagcata tacatgatcc gttactcgat
    18901 tgaagaatcc actgcgaata gggtaatgaa ctttcaggtc tttgattgtg attaattctg
    18961 tcatctacac ctctcctttt tcgtctctaa agtggaaatg cttatagcag gagcaacgta
    19021 ccaagtggtt cggtgcgact tcatgaagga taggctcttc ttcatgatcg cttgctggta
    19081 tccatggaat acgtggtgca aaccgacaac ctttacgagg catgtttttc agagaaggaa
    19141 cgacaccttc gatcacatgg agttctgcat catgatttcc ttcttgtggt atagagttca
    19201 gtaaagagcg tgtataagga tgcttaggat gatcaaacag ttcttttaca cctgctactt
    19261 caacgaactg tccgccatac atcactgcca ctcgatcagc catttctgct acgactccta
    19321 agtcatgagt gatcaagatg atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat
    19381 caagaatctg tgcttggatg gttacatcta gggctgtcgt cggttcatct gcaatgacga
    19441 ttggaggttt gcaggcgatt gcgattgcga tgatgacacg ttgtctcata ccgcctgata
    19501 gttcatgggg atattggcgt ccaactctcg caggattagg aatccctact tgttctagca
    19561 attctagtgc acgagcttga cgctgttctg cattcatctt tgtgtgatag gaaagtcctt
    19621 ctttgatctg gtcttcgatt ctcatgagag gatttaatgc agacaacgga tcttggaaaa
    19681 tcattccgat atcatttccg cggattttat tatagagcgt ttcgttcagg tcgattaaat
    19741 tcagatcgtt atataaaatc tcacctgtaa tttttgtgtt attaggatca agtaatccca
    19801 taatcgtagt agccaaggta cttttcccgc acccagattc cccaacgatt gctaaaatct
    19861 cgtttttgtc taaagtaaat gaaacgtcat ctactgcatc gtaatagtca tctttgagtc
    19921 ggaatcctgt atgtaggttc tgaacttcta ataattggtt atcggacaat cgaatacccc
    19981 tctctgcttc ctaaatggaa gtttttctta ataaaaaagg acattacaca gaataaagcg
    20041 ttttataaca aaggttattc ttatctttgt gtaatgaatt attaataatt atatagaaaa
    20101 tcgctttact ttgcaacata atttctgttt atcgccagaa ttatcagaaa aatgactttt
    20161 acgtgtcaaa aagattaaaa aaagaaggat aaaaagctgt atgcagcttt ttatccttct
    20221 ttgcttttat ttgaaattat ttaagatggc tgctgatata atctactgcg ccaccgactg
    20281 tttcaatttg ttcagcgtct tcatctgaaa tttccgtacc gaattcatct tcaagctcta
    20341 aaacgaattc cataatacta attgaatcag cttttaaatc atctttgatg ttcaattcat
    20401 ccgtcacttt atcggtatct atctcaaaat gatttgaaat aattttcgct actttgttaa
    20461 atacttcttc gcgagtcaac tttttcacct ccaagaatca tacacttgtc aattacctac
    20521 accatggcta attttactgg catttttcag gaatgtccac ctttttttca atggaacgga
    20581 ttgcttcaga tatccaaata ctattcttcg cttttttcaa attgttcgac taattgtcca
    20641 actacatctg tctcaagcat tgtgtggatt tggcggatcg tatagcgaac agcatcgggg
    20701 cctgttgctc catgagtttt aatgactggt gctttcaagc caaacaaaac ggcaccgcca
    20761 tgtttggaat aatccatttc tgctttcaaa gaacgtaagc cgtcttttaa aagcatagcg
    20821 cccatttttc ctttgatccc ttcatttaga atagcagatt ttaatagatt catcatattc
    20881 attgctgttc cttcaataga tttcaaaaca gcgttgcctg taaatccatc tgtgaccact
    20941 acatcagcca caccattgag tagctcacga gcttctacgt ttccaataaa attcaagctt
    21001 gtttcattct gaaggagttc ataggctttt ttcgtcactt cactaccttt tgttgcttct
    21061 gttccgttgt ttaagagtgc gacacgagga cgtccgatac ctcggacttt acgtgcataa
    21121 aaagaaccta aaataccgta ttgcaacaaa tgttctggtt tgttttctgc gttagcgcca
    21181 agatccagca tatcgaaacc ggcaccttct ttccctacta ctggcaaggt agacatcaag
    21241 ccagggcgtt cgatattttt gatacgtcct acgatgaaca accctgctgc tagtagcgcg
    21301 cctgtattcc ctgctgaaaa tacagcatct gcttcaccgt ttttcactgc ttgtgcagca
    21361 aggaccattg atgcagtctt ttttcgtcga attgctttta ctggctcatc atcactattg
    21421 attttttcgt ctgtatggat aatcgtgatg tttttttcat ttgttaagta tttcttgatt
    21481 tctgcttctt ttccgtaaag taagaattct acttctggaa aatcttgctg cgctaaagcc
    21541 acgccttcta caattgcttg aggtgcgtga tctccgccca ttgcatctac ggcaattttc
    21601 attgtttcca ctcctttttc cgtcatcagt ataaagtata gcacagtctg ctaatcgaaa
    21661 taacgattat cctgattttc tatttggagg ctttgcgcta aaggctgata ctcaggaagc
    21721 atctgccaat cctttatctg ccaaagggct gcagcctctt ctctggctac ctccaaaaca
    21781 tttccatccg tcaccaagtc agcaatcgca aattgaggca gaccagattg acggaagcca
    21841 aatacttcac caggaccacg taattccaga tctttttcgc ttagtacaaa accattggtg
    21901 gtctctgtca tgattttcat ccgttccaca cctagttcat ttttaggatt agcaactaag
    21961 atacagtagg aagcatcact tccgcgtcca acacgtccac gaagttgatg cagctgtgcc
    22021 aatccaaatc gatccgcatc catgatcagc atgacggtcg cgttcggcac gtttacccct
    22081 acttcaatta cagtagtcga aactaatatc tgcatctggt tgtctttgaa ggcttccatg
    22141 attacttctt tttcctgatt cttcatttta ccatgtaaca agccaacttc atattttggt
    22201 gcaaatagtt cacgaagttt ttcatagatt tccactgcat tttttacatc taatgcttct
    22261 gattcttcaa tcagcggaca gatgacatac atttgatgtc ctcttgccag ttcttttttc
    22321 gtccaatcca gtacactgtc caattgaggt gttcttaccc agcgtgtttc gatcgggata
    22381 cgtccagctg gaagttcatc aatgatagat acatccatct caccatatgc tgtaatagct
    22441 aatgttctag gaatcggagt cgctgtcata aataagacat ctggtcgcca gcctttttca
    22501 cgaagtactt tccgctggtt cacgccaaaa cggtgctgct catccgtaat aactagacct
    22561 aaattctgaa attcgactcc atcttggatc aatgcatgtg tgccgatcac gatgttaatt
    22621 tccccttgct ctagtttttc aaggagctca cgacgttctt ttgctttcgt tgaccccgta
    22681 agcaatgcga tggttacttc attgggatca aacagctgtt gcaagctttc catatgttgt
    22741 tgtgccaaga tttctgtagg caccatcaac gcaccttgga aacctgctgt aacggtagcg
    22801 tataacgcaa tcgcggcaac cactgttttt ccgctaccta catctccctg caaaagtcgc
    22861 tgcatatgct gcggactgcg aagatcccga cagatttcat tcgttacacg tttttgtgca
    22921 tttgtcagtt caaaaggtag cttttgagta aattctttca atttttggac atcataaagg
    22981 atctccaaac cattcgtttc tgctttttct gcttttttca atccttgaag acgcatttga
    23041 aataaaaaga actcttcaaa aacgacacgt cgtttggctt gatggttttc ttcgtggtct
    23101 tttggaaaat gcatacttct tacagcagaa gctcggtcaa gcagtcgata tttctccaca
    23161 aggttgtttg gcagattttc ttcaatcaat gtgccgtatt cttgaaaccc ttttttgatt
    23221 agatcaacta atgtcgtttg acgaattgat ttgttgacgt gatatatcgg cgaaaagtca
    23281 tccattgttt gagaacctaa aatcttcatc ccgttcaatg ctttacgctt tgcatcccat
    23341 ttaccgtata cggcgatttc ctccgataaa atgacttttt cttttagata aggttgatta
    23401 aaaaaagaaa cgttgaacac atcatgttct tgcatcatac gaaattgcaa tcggcttttt
    23461 ttataaccaa atcgattcat cacaggaggc gagactacga ttcctttcaa agtaaccttt
    23521 tcttgatctt ggatctcgtt tagatttctt tcctgaatat cttcgtagcg gaaaggataa
    23581 taagtcagta agtcttcaat cgtttggatg cccaagctgg caagactagc tgctcttttt
    23641 tctccgactc ctgccagcat agtgattgga tcatctaaag aacgcataaa tttttcctcc
    23701 ctcccttaac aaaaaaagga cttggtcagc gatcatacct gatcactaac cgaaggcctt
    23761 ttatgaatga atactgtgtt agaaagatat tctgacacag aaattattca gcagaaaata
    23821 gataaggata gacaggctga tctccttggt ggatctcgat ttccaaatca ggatgttttt
    23881 ctgttagttt ctcagcaaat tcatttgctt gttcaactgt tccttcatca cctgccaaga
    23941 tcgttacgat ctctgtgtcc tcattgatca tttgttctag tgtacttaac gctgttgctg
    24001 tgatatctgg gttagagatc aggatttttc catcgatcat gcccaaaaag tctccttctt
    24061 tgatctctac accgtcaatt gaggtatctc tgatcgcgtg agtgacttga ccgctcacaa
    24121 cgctagacaa catttctgtc atcgtttctt tgttttcttc taaagaagct tgatcattga
    24181 atgaaagcat agctgtcaat ccttgggaaa tcgttcttga aggaacaact gctactggga
    24241 tatctgctac ttctgctgct tgatcagctg ccatgaagat gttcttattg tttggcaaga
    24301 caatcacttt atctgcgttt acttcttgaa tcgctttaac aatgtcttcc gtacttggat
    24361 tcattgtttg cccaccactg ataacataat tagcgcctaa actttcaaac agctcttgga
    24421 cgcctttacc agcagcgatc gctacgatac catacgggat acgtggtgtt tcttttttcg
    24481 caaactcgtt cacttcttca tcgtgttcca aaatcgtctc atgctgcaag cgcatgttgt
    24541 cgactttgat tttgactaat gaaccgaatt tttgtccata attcaagact tctcctgggt
    24601 tttctgtatg cacgtggact ttgatgatct cgtcatcatt gaccacaagt aaagaatctc
    24661 ctataccatc taaatagtta cggaattctt cgtaatcgaa tgtactatcg actgtaggtc
    24721 cttctccgat ttttaccatg atttctgtac agtaaccaaa atggatatct tctgtcgcca
    24781 tttgaccttg gacactgcga tgatgttctg cattgaccat atcatccatt tctgctggag
    24841 aaggttcata tgtttcatcc gcttggaatt ctccattcaa tgcacttaaa aagccctcat
    24901 aaacaaacag cagtccttgc cctccactgt ctacgacacc gacttctttt aggactggaa
    24961 gcaagtctgg agttttagct aaagcacgct ttgccccttt tacaacagct gtcattactt
    25021 caacgacatc atcagtactt tttgctttct tttctccagc tttcgctgct tctctagcta
    25081 ctgtcaaaat cgttccttct actggtttca ttactgcttt atacgcagtt tcaactccat
    25141 gagtgaaggc tgcagcaaga tcggaagcat ttaatgagac aacatctgga atttgcttag
    25201 aaaatccacg aaacaattga gacaaaataa cgccggaatt tccgcgcgca cccatcagca
    25261 atccttttga aagcattgct gctaattctc ctactttttc tgaggttgaa tctactactg
    25321 ccttagctcc actagtcata gatagattca tatttgttcc cgtatcgcca tctggcacag
    25381 ggaatacatt taatgaattg acgtattctg catttacttg cagacgattc gcacctgcct
    25441 ggaccatttc ctggaactgg cttgcactta tttctgttac actcacctag ataatcctcc
    25501 ttcagatcga ctgctgagtt accagccagt ctcctgccta gtctggcagt acacgaacac
    25561 cttgtacaaa gacattaact gaattggcag ttacgcctaa taatgcctct agattgtatt
    25621 tcactttttc ttggacgttg cgggaaactt cagagatttt cgtaccatag ctgataatcg
    25681 tataaacatc tacagcaata ccattatctt cttggcggac aactactcct cttgagtaat
    25741 tttctttacg caaaatctca gtaatattat ctttaatttg atttttgctg gccattccta
    25801 caacaccgaa cacatctgtc gcggcgccac caacaactgt tgcaattaca tcattggtaa
    25861 tttcaatggt tccagctgac gtttgaattt ttacagccat gaatgagcct ccttaacggg
    25921 taaaaaactc ccatttttct tctatcatat tttatcatag cgcaacttga aataaaaggc
    25981 agttacccaa agtactcaaa aaccttgcaa aatttcaggt ataaccttgt ttaatttaca
    26041 attattcttc tcaattgtca attgtatcta tgaaaatgta cttgcaaatt tccattctat
    26101 atgatatgtt agtctggtat gagctgaatt attctattgc tccaaatcaa ggcaaaggag
    26161 gaaattacca tggcaaaagt ttgttacttt acaggtcgca agacaaaaag tggaaacaac
    26221 cgttcacacg ctatgaactc aacaaaacgt actgttaaac ctaacttaca aaaagttcgc
    26281 gttatggtcg acggaaaacc taaaaaagtt tgggtgtcaa ctcgtgcttt gaaatcagga
    26341 aaagttgaac gcgtttaatt gaatgatact tggaccgttc agatagtgaa cggtttttt
//
LOCUS       DAYYAO010000034        26166 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000034 DAYYAO010000000
VERSION     DAYYAO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.036"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..1098)
                     /gene="queA"
                     /locus_tag="ACHARI_001348"
     CDS             complement(67..1098)
                     /gene="queA"
                     /locus_tag="ACHARI_001348"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8442402.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1291..2466
                     /locus_tag="ACHARI_001349"
     CDS             1291..2466
                     /locus_tag="ACHARI_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8442403.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2470..3111
                     /locus_tag="ACHARI_001350"
     CDS             2470..3111
                     /locus_tag="ACHARI_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442404.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3108..4025
                     /locus_tag="ACHARI_001351"
     CDS             3108..4025
                     /locus_tag="ACHARI_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8442405.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4022..4693
                     /locus_tag="ACHARI_001352"
     CDS             4022..4693
                     /locus_tag="ACHARI_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442406.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5234..5722)
                     /locus_tag="ACHARI_001353"
     CDS             complement(5234..5722)
                     /locus_tag="ACHARI_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8442407.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5923..6189)
                     /locus_tag="ACHARI_001354"
     CDS             complement(5923..6189)
                     /locus_tag="ACHARI_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8442408.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6189..6452)
                     /locus_tag="ACHARI_001355"
     CDS             complement(6189..6452)
                     /locus_tag="ACHARI_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8442409.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6973..7113
                     /locus_tag="ACHARI_001356"
                     /pseudo
     CDS             6973..7113
                     /locus_tag="ACHARI_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7255..9078)
                     /locus_tag="ACHARI_001357"
     CDS             complement(7255..9078)
                     /locus_tag="ACHARI_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8442410.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9676..12381)
                     /locus_tag="ACHARI_001358"
     CDS             complement(9676..12381)
                     /locus_tag="ACHARI_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8442411.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12687..13634)
                     /locus_tag="ACHARI_001359"
     CDS             complement(12687..13634)
                     /locus_tag="ACHARI_001359"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8442412.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13824..14093
                     /gene="rpsN"
                     /locus_tag="ACHARI_001360"
     CDS             13824..14093
                     /gene="rpsN"
                     /locus_tag="ACHARI_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8442413.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14269..14631)
                     /locus_tag="ACHARI_001361"
     CDS             complement(14269..14631)
                     /locus_tag="ACHARI_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8442414.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14642..15763)
                     /gene="alr"
                     /locus_tag="ACHARI_001362"
     CDS             complement(14642..15763)
                     /gene="alr"
                     /locus_tag="ACHARI_001362"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8442415.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15779..16129)
                     /gene="acpS"
                     /locus_tag="ACHARI_001363"
     CDS             complement(15779..16129)
                     /gene="acpS"
                     /locus_tag="ACHARI_001363"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8442416.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16259..17770)
                     /locus_tag="ACHARI_001364"
     CDS             complement(16259..17770)
                     /locus_tag="ACHARI_001364"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8442417.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18092..19444)
                     /gene="murF"
                     /locus_tag="ACHARI_001365"
     CDS             complement(18092..19444)
                     /gene="murF"
                     /locus_tag="ACHARI_001365"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8442418.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19597..20673)
                     /locus_tag="ACHARI_001366"
     CDS             complement(19597..20673)
                     /locus_tag="ACHARI_001366"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8442419.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20869..22614
                     /locus_tag="ACHARI_001367"
     CDS             20869..22614
                     /locus_tag="ACHARI_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8442420.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22894..23331)
                     /locus_tag="ACHARI_001368"
     CDS             complement(22894..23331)
                     /locus_tag="ACHARI_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8442421.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23315..24688)
                     /locus_tag="ACHARI_001369"
     CDS             complement(23315..24688)
                     /locus_tag="ACHARI_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442422.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25010..25498)
                     /locus_tag="ACHARI_001370"
     CDS             complement(25010..25498)
                     /locus_tag="ACHARI_001370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8442423.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25520..26122)
                     /locus_tag="ACHARI_001371"
     CDS             complement(25520..26122)
                     /locus_tag="ACHARI_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8442424.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7503 a         5561 c         4838 g         8264 t
ORIGIN      
        1 tttttctttg tcataaaatc tttgtaaagc ttttttgtta ttcaaaacta gcggcttttc
       61 ataaccttat ttcacgaaca tcgcatctcc aaaactaaag aaacgatagc gctcttcaat
      121 cgcatgctgg taagcagata aagtcaattc tcttcctgca aaggcactaa ctaacatgac
      181 taaagtcgat tttggcaaat ggaaattagt tgagaaagct tctaccactt tgaattcata
      241 gccaggcgta atgaaaatat cagtccatcc gctgtctgct ttgatttctc cattaaattt
      301 cgttccaatc gtttctaatg ttcgaattga agttgttccg acagccacga ttttcccgcc
      361 atttttacgt acttggttca atcgctcagc cgcttcttcc gtcaaacggt agaattcact
      421 atgcatatgg tgttcttcaa ttttatctac gctgacaggt cggaatgtac ctaaccctac
      481 atgtagtgtc agatagacta attcaacacc ctttgcttcg atctcatcaa gtaattcttt
      541 cgtaaaatgc aatccagctg ttggcgctgc agcagaacca ttttctttcg catagacagt
      601 ttgatagcga tctgggtctt ccagacgttc tttgatatat ggaggcaatg gcatttctcc
      661 caatgattca agaatctcta gaaaaattcc ttcatactca aagtcaatga tccgtccgcc
      721 gtgttccaat tcttctttca ctactgcttg caatctgcca tcaccaaaag agattcttgt
      781 cccaactttg gcacgtttcg caggtttgat caatgtctcc cacgtatctc cttgtgtatt
      841 cgttagaaga aggacttcta aatgcgcacc agtatctggt ttttctccat ataaacgagc
      901 aggcagaact cgtgtattat tcataactag cgcatcacca ggctgtaact cttcaatgat
      961 ttcatggaaa tgtttatctt ccatctctcc agttttccga tccaaaacaa gtaaacgaga
     1021 atgatcgcgg tcttttaatg gtgtttgtgc gattaattct tccggcaagt caaaatcaaa
     1081 atcttctgtt gttaacatat cgtcactcac tttctatcat gtgcttcatc attctatcat
     1141 gtttttcgtt ctgttggcaa aatcagcact ttttcacttc tattttttgt tttcctaaat
     1201 aaaaaaacta ccctatttgt attttttcat tgttttaggc atactagtaa tagatagttc
     1261 ttcattgaaa atagaaaaag aggtggcacc atgattgaat ttcaaaatgt gtcaaaaatt
     1321 tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt catttgaaaa gggtgagttc
     1381 atttgtttta tcggtacgag cggaagcgga aaaacgacga cgatgcggat gatcaaccga
     1441 atgactgatc caacacaagg gaaaattttg attgatggaa aatccatcca agaaattgat
     1501 ccagtggaat tgcgacgtca gatcggctat gttatccaaa gtattggatt gatgcctcac
     1561 atgacgatcc gtgaaaatat cactcttgtc caaaaattgt tgaaagtcag tcaagaagaa
     1621 cggaataaaa cagctgaaaa aatgattgat cttgtggaat tgccccgtga gatgttagat
     1681 cgctacccac atgaattatc tggtggtcag cagcagcgta ttggagtcgt tcgtgccctt
     1741 gcagcgaatc aagatatcat cttgatggac gagccattcg gtgcgcttga tccaatcact
     1801 cgtgattcct tgcaagacct tgtcaaagat ttacaagagc gtttgggaaa aacgatcgta
     1861 tttgtaaccc atgacatgga tgaagcgatt aaactagcaa gccgtatcgc catcatgagt
     1921 gaaggccgtg tgatccaatt tgatacacca gaaaatattt tacgtcatcc tgcaaacgat
     1981 ttcgtagaag agttgattgg agaagatcgc ctactacaag ccaagccaaa tgcgacaaga
     2041 gtcggcgaag ttatgctaaa tacagcaatc acgattacac cggaaaaatc attacaagaa
     2101 gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt tagttaccga taattcgaat
     2161 gtattaaaag gatttatcga tgtagaaacg ttgaataaaa aacgaggaaa agtatccagt
     2221 gtcggcgaca ttttgaataa acatgttttt tacgtaaaag aatccgccta tcttcgtgat
     2281 accttgcaac gcattttaaa acgcgggctg aaatacgtgc cggtcgttga tgaacagaac
     2341 aaagtcgtag gtatcctgac tcgtgcatca ttagtggata tcgtctacga tgtgatttgg
     2401 ggagaagatc ccgcaactac tgatgttgca gagtcagcta ctcctgaaga atctaaggag
     2461 gtctgaccta tgcagcagtt ttttcatcag cacggttcag aaattctctc aaaaggtttt
     2521 gaacatcttt atatttcagg acttgctttg atcattggta tcatcattgc tgtaccgctt
     2581 ggtgtcttac tgacacgttt tccaaaagct gccacgattg tgattggctt aaccagtgcc
     2641 ttgcagacaa tcccttctct tgctttattg gcattgatga tcccgctatt tggcgtaggt
     2701 aaattacctg cgattatcgc actctttatt tattcattat tgccgatttt acgtaatacg
     2761 tacattggga tgaaaaatgt cgattggaat tatcgggatg tggcaaaagg catggggatg
     2821 actgagttcc aatccatttt ttccgttgaa ttgcccattg ctatgccaac gatcatggct
     2881 ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta cacttgcctc ttatatcggc
     2941 gctggtggtt taggcgattt gatttttagt ggactgaata attaccaacc agatctcatt
     3001 ttggcaggta cgatccctgt aacaatttta gcactattgg ctgattggct tctaggtctc
     3061 ttggaacacc ggctgacacc gatcgcactt agagaggaga atgaagaatg aaacgtatac
     3121 taaaaatgac catggttctt ttctctcttc ttatacttgc gagttgttcc ttccctggtc
     3181 ttgccagcaa tacagatgaa gacaccattt cgatcacagg tggaatcact tcagaagcac
     3241 agatcttagc aagtctagtt gccggaatgg ttgaacatta tacagataaa aataccgcaa
     3301 tcatcaacaa tctggctact acaactatca accatcaagc gatgatgaac ggagatgctt
     3361 caatttcagc ggctcgctac acaggaacag atttaacgac tacattgaac cttcctcctg
     3421 aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga atttgagaaa agatacggtc
     3481 aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt ctttcttgtt cgaaaagaca
     3541 ccgcacaaaa ataccatcta tctaaagtca gcgatctgaa aaatgtagcg gatgaattag
     3601 tagctggtgt agatacttct tggatcaatc gtaaaggaga cggttatgac ggcttccaag
     3661 aaacttatgg cttctccttc aattcgatat tacctatgca gattggactt gtttatgacg
     3721 cagtcgaagc aggaaaaatg gatattgttc ttggctactc tacggatgga cggatcgcca
     3781 gttatgatct agtcatgttg gaagatgatc gtcaattctt cccaccatat gatgctgctc
     3841 cagttgtaga caacaagata ttagctgata cgcctcattt aaaagaagcc ttaaataagt
     3901 taggtcatac catcagtacg gaaaaaatgc aggaattgaa ctacgaagca gataataatc
     3961 tagtggaacc atcagtagta gcacaacgtt tcttacaaga aaaccactat tttgaaggga
     4021 agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt ttctattatt tccaggaaaa
     4081 tggctcctat atctttgcac aatttatccg tcacttcctg atttctatct atggtgtcct
     4141 ctttgcagca gttgtcggga ttccggttgg gatcatgatt tctaggagac gtaaattagc
     4201 aaactgggtg attcgtttgg cgaatatcat ccagacgatc ccttctctag ccatgatctc
     4261 cattttaatc attggattag gcctgggagt caatgtcgtg attgttactg tatttttgta
     4321 ttccctctta ccgatcatca agaatacgta taccgggatg atccaagtag acaaaaacat
     4381 ccttgatgtc ggcaaaggca tggggatgac tgctagacaa cgactattca tggtagagtt
     4441 accactctct gtttctgtta tcatggcagg cataagaaac gcgttagttg ttgccatcgg
     4501 gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt ggagatatca ttatccgagg
     4561 aaccaatgca acagagggca catcgattat tttagcaggt gcattgccaa ctgcattaat
     4621 ggcgatcatc acggactggc ttttaggtat tttggaaaga cggctagatc cagccagccg
     4681 tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg cgaccatttg ttggtcgcaa
     4741 aggctttttt agttctgtca tttttaaggc aataatccac tctgattctt ccctccttat
     4801 gggcaaagag ttgcttttct tagctattta tccattccgt atactccaac ttcatactgc
     4861 caacgggttc atatctgcat ccaccgacta agtttttttc ttatccgcta aggcattttg
     4921 ccaatccatt ttcccatcac tcgtcatgga attcatacca catgatctct aaatactcgt
     4981 ctgcttgttc tttgaattgg gcatcaattc ttacatcctt tggcgtggct ttcatttaag
     5041 ccttttccat cagtcctcga ttgttcgatg ccttcagctg catcatcaaa actggtgact
     5101 gccgtatcgg taacttcgat catcttttat acaatcggga acttgccatc ctcatccttg
     5161 gctaaaaaca tatctagcca ttccagcttc tcgccatggg aggtttgcgg ttcctaaaac
     5221 cggtacacga ttcttatgga ctgcatccgt cgcaactaga cttggcgata cgatgatcct
     5281 tcaccagaag agcacccccg taaaccaatt gatcgatata ttgccaataa gaaaagacat
     5341 tggcgtaaaa gatatcgatc cctcgtgatg cattgccgtt gatactgcta ttcatggccg
     5401 aaagcgccac gactttaact tcttccgttt gagtgacatt catgttcggc aatgtcgctt
     5461 tggatacccg ctctgctaaa ggaacgacac tggtattgta tttcgtatct gtatcttttc
     5521 caaaagacca tgccaacgaa tcctctggga accaataaga cgactccggt tgattgtcca
     5581 ttgctttttt caacatactt ttggctgttt ctcgataaac gacatttcca attgtcttcg
     5641 cctcactttg gcgataatcg atggtcgttg ccactgccaa cactaacaac gatgctccaa
     5701 ttttactgat tttccttgtc acacttcttc ctccttgttt tacataaacc cgtcaacatt
     5761 ccattcctaa actcaaacaa tgacccttca aaggtggaga tataggaaca aattaaagaa
     5821 atcatgcaat aaatccaaga aggttggttt tagaacaaaa aaagagtctc ctttttgggg
     5881 agactctttg ttaactatct aaaaaggttg gctcttctca cttcactcat aatgtgtcca
     5941 ggcagaataa atttctacaa ctttattttc ctcatcaact ttgtaaacca ctcgatgctg
     6001 tctattcagc ctgcgtgagt atctgccttc atgtgtaggt ctcagctttt caaaagactg
     6061 cgtgggcata tatggatctt ccttcaatgt ctgaatcact tcttcaaatt gtttctttaa
     6121 attcgtttgc ttgatttttc ttaaatccac tttcgcagag tttttgattg cgactgtata
     6181 gttactcatc aaagagcttc ccaatcaata tcatcgatat tcgtaaagct actgtcgtct
     6241 ttttcgcggt ctcgtacttt atctaaagta cctgtttgct caagaaaaag ggtttcttga
     6301 atcgctctcc agtcttccag cccaatcaat acagcattgt ttccactacg atcactaata
     6361 atctcgattt cagtatgatt ttcatttacc tctttcaata actgataaaa atttttacga
     6421 gcagtgctag gattcatcgt tgtattagcc attgtctctc ctcctttttc tgatataatt
     6481 atagcgtact tgattaagta cgtcaaaaaa atacaataaa gcacttaaat tcagcaaact
     6541 tttcctcttt tttacataga gaaaaagacc cctttttagg gaggatcttt gtccacgctc
     6601 acttcttact cctttacgac tagcggtagc caaacgtaaa gtaattggta acaacgtatc
     6661 atctttagca aaggtcgaat ccaaagcctg tgagtctatg caacaacacc aatacacgaa
     6721 ggttcctcgt tgcagtgtga cttgattgat atcttgaata acctgaggat acgcacgaaa
     6781 gatttgtaac ggttattcaa aactgatttt ggccgtcaag tcttctactg cctcaaaaat
     6841 gattgcctct ttcgtctcct gtcattcatg ttcactatcg atttgatact tgcttagacc
     6901 gttgggtttg tgaatcacca attaaaaatg gcactgagtt atggtaacaa aaaatggtca
     6961 cttgacgagg ctgagattcc tacttatgaa gacacatggc acttaagagt cgaggtaaag
     7021 gaagaagctg aaaatatagg aggctgtaca ttatttggca aaggattcgg aaattatgat
     7081 caagacatca aaatcaaaat gttttacagc taaataggaa gaataataac atttcatacg
     7141 ctgttcccat ctttaatggc ttatagaggg cacctttagc tgagaataag acaaaacaga
     7201 ccctctagct tcaattaaga gctagagggt ctgtttttga ttttaaaact tggtttattt
     7261 cttcaaatgg tagctatagg tgctgacaac gatattttca cgccaagaca atcgcaatct
     7321 taaccgcaaa cttgtttggt tatggagaat attctgccag cgtctgtttg gtacaaattg
     7381 aggaatcaac acagtagttg tatagttttg tttcgctgca tttttactta cgatatctac
     7441 ataacggata attggatttt caatcgagcg ataagaagag tggacgattg aaaaacggac
     7501 atctgggaaa tgctttttaa attctgcttc gatttctttt tctttttcga catcttcgtc
     7561 tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa cgtgcgtaat tcaacgcacc
     7621 gatatttacc cgtgtcacat tcccgactaa tacaagaacg gtattcccgt cataatcatg
     7681 tagttttgca tcattttcta aacgaagctg ttctgctact tttttataat gatcatggat
     7741 cttataaaaa atgaacataa cgattggcat aatgatgaag aaaggccaaa tgtcccctaa
     7801 ccgatagaca aacaaaatcg caatgattgc atatgagatg aacgcacccg taatattggc
     7861 aatcgatttg cttaaccatc tttttccttc ttttttccat ttgacgacca tccctgtttg
     7921 agataaagca aatggaatga acacgccgat agagtataac ggaatcaagc gttctgttga
     7981 gccatggaaa ataaacagca atacgatcga acctagcgct agtgtgataa tcccattgga
     8041 atagcctagt cgatccccac gatcttgata catgtgaggc atgaatttat cttttgccag
     8101 attgtatgca agaactggaa aagcagaaaa accggtgtta gcagcaactg ctaaaatcag
     8161 cgctgtagca aattgaagga cgtaatacaa aataccatgt ccaaagacag ctttaccaat
     8221 ttgagacaaa acagtgactt ctttttcagg aacgatccca taccaatagt tgataaatgt
     8281 gatccccacg aagaagaaac ctaagatcaa tgccatcatc gtcaatgtag cagcggcatt
     8341 ttttgctctt ggcttcttaa agaatggtac agcattacta atcgcctcca ccccagttaa
     8401 agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg atagaaacac ccggcacagc
     8461 cgctcctgga agtgcagtag cttgcaatgg cacagcacct gtcacgatat tatataaacc
     8521 aacaacgatc agtaacgaga tcacaaggat aaatgtgtac accggaaata ataggaacga
     8581 tgcagattcc ctcagcccac gaagattcag catcatcagt agcaaaacga ttgtcacaga
     8641 aatagcgact tgatgaccgt agagtgctgg caccgctgat gtgatcgctt cagcacctgc
     8701 tgagacagat actgctactg tcaacatgta gtcgatcaat aaagagccac cggaaatcaa
     8761 gccagcattc tttccaagat tctcactgct gacgacatat gctcccccgc catgaggata
     8821 agcatggatg atttgacgat atgatagcgt taatgaaatc aataaaataa taacaaaagc
     8881 ggcaatgggt agcgaatacc agatggctgc agcagacaaa gcgactaaaa cgacaacgat
     8941 ttgttccgta ccatatgcaa tagatgataa cgcatcagac gacaataatg ccaaagcagc
     9001 aaatcgggtt aatttgtgct catcattttc agcagatttt aaaggcttac ctactaacaa
     9061 cctttttaag taatccaatt tctaaactcc tttacacaca attttacttc aagttttata
     9121 gtcgaaaatt tatagtaaac aagatgttat agtacgctca ctgaccttaa aagtcaataa
     9181 actttaagaa aataattgga aaaaaacaga aaaatatcaa gcgctttttc aaacgcttta
     9241 atacagcaaa aatctaacat ttttttatgg gctttttctt tgaattctat aataaaagga
     9301 tagaacttga cttagaaaat caagttctat cctttttgat atcttaactt ttggaagaga
     9361 gatgtaaggc tgctttctac ccatcattct tactactcga agcatagcgc agttgtcaca
     9421 acctcttgat aaacggtgtt taaaagcttg catctgcggt aataagccca accaaattaa
     9481 aaacataaga agctgttttt atttggtcgt tcttattact tgatgcagag cagctttttt
     9541 acaacctctt ggtacacggt gttcaatcag ctgaccctcg gtattagctc aaaaaaacgg
     9601 caaaatatga ggaactattt ttccattttt ttctctaata ctcaggtcaa aacactgatt
     9661 tcacaacctc tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt
     9721 cattttttct tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacaa
     9781 accgatcatg atccagattg ctttcgtagc atttggccaa taaattcctc ctgcagattc
     9841 ccgtaacaag tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt
     9901 ccctgagact tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat
     9961 aatagcgatc cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat
    10021 gaacgctaag gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac
    10081 tcctagtaag aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa
    10141 aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt
    10201 tcctaaatag tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca
    10261 tagtgcagta taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt
    10321 ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct tttgttgcgt ctagtttcaa
    10381 taatttgatg acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc
    10441 tttttggata ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt
    10501 cacagctgtt tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc
    10561 attttttagt gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg
    10621 aatgatcggt aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt
    10681 atccatattt ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat
    10741 ttctttttga tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgagct
    10801 gagaagcgaa tcaaagtcga tctggtccgc tttgctcagt gcgccttgtg cagtcccaat
    10861 cgtatcgatg acttggtcta gtagagaagt gacagtggaa ctgatttgac ctgtattgat
    10921 accagcaatg atgccattga tattattaac aacggcatca atttgatcta gcaattcatc
    10981 aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag
    11041 attgcttagc aagggtttta attgatttag ctgatcaata attggctgta atttcgtatt
    11101 tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg
    11161 ttgagatatt ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc
    11221 agtcagctta ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt
    11281 tgccacttgt ccaattgact tcaatgtaac atctacactg tttgtgatgc ttggtatcgc
    11341 atctttcagc tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc
    11401 taaatttttc acatctggca agatatcttg tacttgttgg atgatcgtca agccttgttt
    11461 tgcttcatcg attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc
    11521 tgctagttgc ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat
    11581 ttttgcttga tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat
    11641 ctcatctact ttagggatgt aatccacgaa ttcatttgct ttattcagtt tttccttgat
    11701 ttcaggtagt tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa
    11761 attttcatct gttgataaaa tcatatcttt aactttattg atacttacta ggttcgtatc
    11821 gatgtcgtaa cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa
    11881 ttcatttgtg atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat
    11941 cgcattgatt ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt
    12001 aaaactcagc agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc
    12061 ggaacggact ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt
    12121 atttttatga agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc
    12181 tgctggcttg tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc
    12241 ccataatgct ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat
    12301 cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag
    12361 cgtatttttg attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta
    12421 ccatgtattt catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata
    12481 tcacggcaat tgtctcattg taatctctta catattgaga aataattaag gattacagcc
    12541 tttactttat catgaaataa aagggaaaga taattttatg ccaaagtttt tatctattat
    12601 acgtatacgc aaaaaagcta aaacattttt tgtctttgcc cctacaaaaa tgtttcagct
    12661 tttttcgttt tttattcctg tatttcttat ttttcgaatg acaggatgat tttcccagca
    12721 ggaggatgtc cttcaactag ctgatgtgct tggatgacat gcttaagtga agcagacaga
    12781 ttcattgaaa taaaaacatg aaaggctcct ttttggtagg ctttgattat gccagcaaag
    12841 gcttctgcat ctagatattc ctttcttgga acaaacgatt cataaaagcc ttcctttttt
    12901 tgccgcaaat ctagatctgg gaggtcattt aatgcaacat acctgccgcc tggtttcatg
    12961 atctggatgc cggtttctcc tttgatactt cctttcgtcg catcgattac aaggtctgcc
    13021 tgatcagcaa attgcagacc tggatttgtt ttatcataag cagcaaatgc tgaagctcct
    13081 aattttttta ccttttcctc atttttactg gatgcactgg ttaagattcg gattcccttt
    13141 tcatggagaa gctggatcag acttgacccg accgcacctg atgctcctag tatcattaca
    13201 gtatctgtcg gctggatttc cgtcaaatgg ttgattagat tataagccgt aattcctggc
    13261 gtgaccattc cggctgcttc ttcccaactc attttatctg gaatttttgc tacttttgct
    13321 gatggcaaca cgacttcttc tccgtaagtc ccgccgactg catggacagc tactcgatca
    13381 cctacatgaa cagttgtgac atctgaacca atctctgtca caataccagc gccatcatta
    13441 cctggtacat aagggaattt caatgttcgt atctctttca tttctcctaa acgcaaggca
    13501 acatcataag gattcacact gaatgctttt atttctacac gaacatgtcc agaaccgact
    13561 tctctgggat gagcatcgat ttcttcaaaa acatcttgtg cttctccgta accgttaatt
    13621 gcaaatcttc gcatggtcac catctccctt tttctttaag tttacgttta tgcctttatt
    13681 ttgacaaaaa aaatgtcttt tcgttttggc gcatgattca aaacaaaata gttgtctttt
    13741 ttcgcgtaac gtgttaaatt atttatctaa aagcgtaaag attacgattt acgctaataa
    13801 aaaataattg gaggaatttg ttgatggcta aaaaatcaaa gattgccaaa gcaaaaaaac
    13861 agcaagaaac gattgaacgc tatgctgctt tacgccagga attaaaagcg caaaaagatt
    13921 acgaaggctt gcgtaaactt cctttagatg ctcgtccaga acgattgaaa aatcgtgatt
    13981 taatcgatgg acgccctcgc ggatacatgc gtaaattcgg gatgtcccgt ataaaattcc
    14041 gtgaattagc acataaaggt ttgattccag gagtaaaaaa agccagctgg taatccaagt
    14101 ctggctgtaa tgtaaaggag gagggcgcat ggaccactcc ttggatcatc aaaaaagata
    14161 cgttcgcttc ttctagcaga aatgctggta ctagaagcag acgtatcttt ttttacgcac
    14221 agctggtcct tttttatatg taaaggctgc atggttactt cgctatattt atttagtaac
    14281 aggagtcagt gcttcgatac ccacactaat tttcaatgct cggtcaactt ctgccataat
    14341 gggtcgatcc aaatggcaga ccttttcttt caatcttgat ttatcgatcg ttctgatctg
    14401 ctccaacagg ataacggagt ctctttctat tcctgttcca gtagctttga tcccaatgtg
    14461 agtcggaagc ttaggtttag ccatttttgc cgttatggcc gcaacaatga tcgtcgggct
    14521 aaaatggtta cctagattat tttgaataac caaaactggg cgtgttccgc cttgctccga
    14581 tcccacgact ggagaaagat cagcaaaata aatatctcct ctttttacca taggatcctc
    14641 cttattcttg atattctctt ggtatccgct gtcctaatcc gcaagccact tcatagtgga
    14701 tcgtacctaa ttgatctgct atatcctgca tagtgatttc tttgtctgca tttttgccga
    14761 taagcgtcac ttttgttccg acaggaaatt cttgaggcaa tcggatcatc agttgatcca
    14821 tacatacacg cccaatcgtt tcacagtagt tcccttctac caatagagaa aaaccctgca
    14881 tctttcgtgg ccagccatct gcataaccga tcggtatcgt tccgatccac tctgcttcag
    14941 gagtgatata tgtttctcca tagccgattc cttcacctgc tgggagtttt ttgacttgaa
    15001 tcaattcaga aaccagctca agtgctggct gcaatggata aacttccggt agtgcgtgcc
    15061 cagagggatt aagtccgtac attgctactc cataacgaat catattgccg attgtttcat
    15121 catgccataa agccgttgca ctattgctga catgtacata tcgaggaagt tcgggaagtt
    15181 ttttcagcac ttctataaag cgttcttttt gcagattcca atatgtatca tctgcctgat
    15241 ctgctgtaga aaaatgggta aataaacctt cccattctaa tacacgactt tctttgatga
    15301 atgcagctgc ctcttttact tcttcaggtg tacaaaatcc gatacgaccc atcccagtgt
    15361 ctgcttttag atgaatcgaa agaggcgtct ctagcttact tttctctaaa acagcagctg
    15421 ctgcttgcaa ccattcccgt gtacctgcag ttactgataa atcatgagca acagctacag
    15481 tcagtgcgtc gtaaggaacc atattcaaga taaggatggg ctgagtaaat ccagcttctc
    15541 tcaactcgac tccttcatct aaatttgaaa cacaaaagcc cgaagctcca cctgcaactg
    15601 ctgcctcagc tgtttcaata gctccatgac cgtatccgtt cgcttttact actgcaaaca
    15661 attcttttcc ttgcggaagt cttgcaactt catttgctac attttcagtt atagcttttt
    15721 tatgtatcac tgctttcgtt gggcggtgcc aagcgacaac catcgttttt ccttctttct
    15781 agtcttccaa aataatttgt gcaaaagctg tttcatttgt atgagtaata ctgacccata
    15841 cattgccttc atgaggtgaa cgcgtcacaa caggctgacc attttcattt tttaaaatct
    15901 cgacatcttg aaaggtaact tttccgatcc cagttcccca agctttagag aaagcctctt
    15961 tgcaagcgta acgcccgcct aaaaattcta cttggcgatg aaaaggcaag gactggaata
    16021 gtttcatttc atcagaggtc aagattcgtg caatgaattt tggtttttct tcgatcagcc
    16081 gagtgatcct aggaagttcg actgcatcga tcccaattcc ttttatcata tgcttttctc
    16141 ttttcaataa atttagaaca ctctcattct accaaattat aaccattttg ccacaaataa
    16201 gtccacaaat aacaaaaatg aaagtttctc ttaaaaaaga gaaactttca ttttatgttt
    16261 aatcgttatt tgaacggatt acaaatccgc gttttttatc attgtgacgt ttaccgccat
    16321 ctttttggct gttattattt ttgctataac cgccttttga tttgtttcca cgataattac
    16381 cgccgtcacg acgattacgg ttgtttccgc caccacgttt gccattttta ttgtatcctt
    16441 ttttctgcat tggcaatggg cgttctggtg tgatcttcac tggcactgca tcagctggat
    16501 cttttgctgt cgtttttagc aaaagtgcaa ctaaatcttg tgcagaatat tcttctaata
    16561 atttatctgc tgtttgcagg tatttatcta gaccattttc tgcaagtttt gtttcgattt
    16621 gttcaactgc agcacctaat tgacctttga atgcttcttt ttcggttggt ggacgtaaag
    16681 tcgtcatccg ttttttcgtc aagttttcaa tcacatgcaa gtagtccatt tcatttggtg
    16741 taacgaaagt cacagacata ccgccttttc cggcacggcc tgtacgtccg atacggtgaa
    16801 cgtagctttc tggatcttga gggatatcat agttgtacac atgagtgaca cctgaaatat
    16861 ctaatccacg tgcagcaacg tctgtcgcta caaggatatc taaatgaccg ctcttaaatg
    16921 aacgcaatac gctcattcgt ttttgttgag acaaatcgcc atggattcct tctgcacggt
    16981 aaccgcgtgc ttctaatccg cgtgccaatt cgtctacacg gcgttttgta cgtccgaaga
    17041 cgattgttaa ttcaggtgtt tgaacatcaa acaagcgagt catgatatcg aatttttcgt
    17101 attcttttgc gcggacatag tattgatcga tcaagtcagc tgtcatttct ttcgctttga
    17161 tcttcacatg atgtggattt ttcatgaatt taacgccaat attcttgata gctggcggca
    17221 tcgttgctga gaaaagtaat gtttgtcttt gatcaggaac ttgtgaaatg attttttcga
    17281 tatcttccaa gaagcccata ttcaacattt catctgcttc atccaaaact aatgtttgaa
    17341 cggttccaag tttcaatgtg tggcggttaa tgtggtcaag catacgtcct ggtgtaccca
    17401 caacgatatg tggacgatct ttcaagccac ggatctgacg gccaatatct gctccgccgt
    17461 aaacggcttg tacacgtact tttttgtcgc gccctaaacg atataattct tcttgtgttt
    17521 ggatagctaa ttcacgtgtt ggtgcgataa ctaaaccttg taattcgtga cgatctggat
    17581 cgattttttc taacattggt aatccaaatg cagctgtttt ccctgtacct gtctgtgctt
    17641 gtccaataac gtcttttcca gctaatgcta gtggaatcgt ttctgcttgg ataggcgttg
    17701 cttcttcaaa accagcgcgt tcgactgatt tcaacaattc tggtgataat tctagttctt
    17761 taaatttcaa atgaatcctc ctatatggtt gttaactgag gatgtagctt ttttgctacc
    17821 ccgtcgattt gttcggtcaa agtgtattgt ttctttttga cagtggccac gcacgttttt
    17881 atccattctt agcccctgtg gcaccaccga aaaatcactt tgcgaagttt aggcattaga
    17941 aaagccaaac gggtcgcgtt cagctttatc attattatga catctggctt caacaagttg
    18001 tttctatggc aaaaccagtc caatttctct tcatctttcg gcaataggtt attctaacat
    18061 agtttttcgt tttctgcaag tttacagatt tttatttcat ttgctgaagt tgttctacca
    18121 cttcaaataa gcccatgctg ttgctgcctt ttagcataat actatctgtt gcttcaagat
    18181 tttgtttgat agcatcaatc aatttattct tttctttttc caaatagaac acaggtagtt
    18241 gggggtattt ttggttcaat tcttctttta gtgcatgcat ttgttcccca taaaggaaaa
    18301 tcatggcgaa tttctcagga tcgatatgtt cactcatttg cgcatgcata tttaaagact
    18361 ctttccctaa ttccagcata tctgctaaca cagcgattct gcgtcctttt gttggcagat
    18421 ttttaaaagt atctaatacc agtcccatgg ctgttggatt tgcattgtag acatcgctca
    18481 aaatagctgc accatttcct gccgttagcc attcggttct gttttgagtg atagaaacat
    18541 gggcgagccc ttggcgtatc tcaggtatgg acagtccaaa gaagcgcccg atcgtatacg
    18601 caatcaaagc attcgttaca ttataacttc caggcagcgg gatcgtaaat gtttctcctt
    18661 ctactacaaa atcggtttgt gcttttcctt ttgctgtcac ttccgcatga acatcgccat
    18721 ttccaatacc gaatgttgtg actgtttgta ctaatgttgc cgttaaaggt gtcagcaacg
    18781 gttcatctgc gggcacaacg agtaatccgt ctgatacaag tccgtcagtg atttccattt
    18841 tggctttagc gattccttct cgcgacccaa gattttctat gtgtgcttca ccgatcattg
    18901 tgattgctgc agcttttggt tgagccataa gtgaaagttc cgtgatttca ttggcgtgat
    18961 ccatgcccat ttccaaaacg atcttttctg tatcttgggg catgtgcaaa atcgtgtaag
    19021 gaagaccaat attattgttg taatttcctt gcgtcttata tgttttgtat ttttcagaca
    19081 agacagacgc ggttagatct ttcgtggtgg ttttaccatt gcttcctgta attgcaacga
    19141 cttctgcgcc tactttttcc aagtaatagg ttgccaattg ctgcatggct ttcaaaggat
    19201 ctgctacagg aataactgga aaattttttg gagcttgttc aacaggcaag ctccaaaatg
    19261 aagcagatgc tccgtttgtg atcgcttgct ggataaattc atgtccatct cgcgtaccag
    19321 caagaggaac gaataaactt ccttcggtta tcttccggct gtcaaattcg acattggtaa
    19381 tctttgtcag tgcagagcat tcgctgccca cggcttgtgc aatttcttgt attgttagat
    19441 tcattgagat ctctcctaaa aaaggttgtg aaaaaagcgt ttggcctaaa cggcgggttc
    19501 gcatgactct cacttccaag taaatttctc tattcgtatt tagaagctag aatgaacgcg
    19561 gttacgcaat cactccagcc tcttctttat ttctctttat tcattttttt caaaaaaaga
    19621 ttgacgctga tggtatcgat tcattcctaa ctggatcagt tcttcaatca aatcaccgta
    19681 tttcaagccc atattttccc ataagagtgg gtacatactg aactccgtaa atcctggcat
    19741 agagtttaat tcattcagga ataattcatt tttatttgtc aaaaagaaat cgcaccggct
    19801 caatccgctt ccacctaaca tcgtgtaagc taacttcgcg tactcttgcg ctttttgata
    19861 aacttcttct ggcacttcgg ctggaatctg catttcgatt ttattattga tatattttgc
    19921 ttcataatca tagaatgcta cgtcttttac gacttcacca ggcaaagtcg tccgaacgtc
    19981 ttcatttcct aatacagcaa cttcgatttc gcgcgcttca attccttgtt caacgattgc
    20041 tcgagaatca tactgatagg ctgttgctaa agcattttgc agctcttctc ggttttctgc
    20101 ttttgtaatg ccgacactag aacccatatt cgccggtttg acaaacatcg gataaagcaa
    20161 agaaccttca cattgatcaa atactttttt aggattttct ttccattgat tcttaagtac
    20221 tggtacataa ggaacttgcg gcacaccagc agcttgtaaa atatacttgg tcatgatttt
    20281 atccatggca catgcactgg tcaatacgcc tgcgccgaca taaggcatat tcaatgtctc
    20341 taagaagcct tggatcgttc catcttcccc gtttggccca tgtaaaactg gaaaaacgat
    20401 ggctccttct tctttgattt cgcccggatt gatcactttt cctgtaaagc cttcctctgt
    20461 ctgtccactt gggtcccatg aaagatgcaa gacatctttg ctggcaggtt tttctgttag
    20521 taatggacct ttgacccatt gtccttcttt tgtaataaat acgagttgaa cttggtaata
    20581 attataataa atggcattta aaactgaaaa tgcggaaaga atggacactt catgctctgc
    20641 gctgcgtccg ccatatagta aagtaatctt caaaagattg tcctcctgta ttctaaatcg
    20701 ttcagtgatt tactctcata ttttagcaca aaacaaaaat aaacgaataa cttatttata
    20761 aaaaaacgag ccagcttcta aaagaacgag cgtcatttat tttttgtcag ctagattatt
    20821 tgtcccgttt tttataaaaa gttatgataa agaaaaggag ggatgatgat ggcgcaaaca
    20881 aatccacgca gccgcttggc agaaatcatt cgagtgttca tcaattacaa tgtagtgcca
    20941 aactttgtac agcaaaaaaa tcccgaacag gtaaaaaaag cctttgaaga actaggcccc
    21001 accttcatca agataggaca gatgctttct gttcgagaag atttgctttc ttctgccttc
    21061 actcaaacat tcaaaacatt acaagatagt gtacctagcg acacattttc aacggtcaaa
    21121 aagacgatcg aaacagaact ttctctttca ttatcagata tattcgatga tttttcaaaa
    21181 tcgccttttg cctctgcttc tatggggcag gctcatcgtg ccaagctgaa gaacggtgac
    21241 gcggtagtcg ttaagatcca acaccctaac atcgccgaag agatccgact tgatcttcaa
    21301 ctatttgaac gagcgatccc tttgatcaaa tacattcctg aaactagcgt agttgatcta
    21361 aaaggggtac tccaagaagt aaaacgttcc ttgatcaatg aaatggactt cttaaaagaa
    21421 agtcaaaatg gcgaacaatt ctatcaaaag aacaatggct ggaaagagat ccgcagcccc
    21481 aagatttatg acgctttttg ttcgaaaaaa gtgatcgtga tggaagaaat gtctggtaaa
    21541 aacttgaatc atttaatgaa cgccgaaaac aaaactgaaa cttttatcac tggaattcaa
    21601 aacaagcagc tgaaacaaga agttgcaaag cttttagttg agaactttat gaaacaggtg
    21661 tttgatgatg gttttttcca tgctgatcct catccaggga atctgctatt ccatgtacta
    21721 acaaaagaag aacagacaca agctagccga aaaactgaaa cagtccatga aaaagagttc
    21781 ggttcctttg cttttcgagc ctccacttct gcagaagacc ctgttgcgcc atataccatc
    21841 aactacatcg attttggtat gatggggcat ctttctgcag gacttaggca aaaactcaca
    21901 caagccgttt tagcgctgta tacgaaagat gcttatcgaa tcgaaaaagc ggtccttcga
    21961 ctttgtcagc aagaaggctc gtttgatgaa agtcggttcc accaagaact gacaagtttt
    22021 cttgaacagt attatgattc tcctattgat gaaatcaact tgcaagaagt attcacacat
    22081 gtcgtgacga tttgtcatca aaataatctg cagttcgatc gtgatatcac gcttttgctg
    22141 aaagcatttg ggacattaga aggggtgatc cgcgtcctcg atcctgaagt ttccctcatg
    22201 gaagtcgctt cgccttttgc tcaacattat tttctcactc atctggatgt agaagataca
    22261 ttgaaacaaa gcggattaga cttattggaa gggatgaagg ctgcgcctaa gatccctcaa
    22321 cagctccacc atctattaga aatgtggact tcgggacaag gaaaagtcaa tttggaattg
    22381 aaaaagcaag acaaattatt gagcaggatc gaaagtatga tcaatcggct tgttttcggt
    22441 atgattttag ctgcattgat cgtagggtcc tccctgcttg tccaagcagc acctgtagaa
    22501 aacgcagagg tcgtttcctt attagggata tttacgtatg ctattgccgc attcgtcatt
    22561 atttttttgg caatcgatgc attgatccaa atgtataaga agaggaaaaa gtagaaaaag
    22621 gttgtgacag aaagaatcag cttcaagaaa taagaagggg ttttacataa attgtttctc
    22681 aaatttttgt gaaaattcgg cttatttttc gaagaggcta cttctgaaac accgagaata
    22741 aaaaatgtga aaaagctgtt ctgcacccag taataagaac aatcaaaaag ggaacatgga
    22801 acacatttat cctgtttccc tgttcccttt ttgatgtatt cgagcataaa taaaatacct
    22861 tcgttaccta ctatcttctg tacttggtaa accttagtca attgcctttt ttaacgcttg
    22921 acgaaaagac atatgatctt tctccgtaaa gggttttgga ccctttgtcc ttatgccagc
    22981 ttttcttaac tttccactat gataacgttg ttctagaagc gtgccgatat tcgcttcgtc
    23041 aaattctttt ccagagtgat ggaacactcg cacacccttc ggcaatacaa gtgaagctaa
    23101 accataatct tgcgtgatca aaaagtcatt cttcttaacc ataccgacga ttttataatc
    23161 cgcactatca gctccgcggt ctacgtagac gatttggaca tgattagcgt attctttggt
    23221 cgtataatgg tccacactgg tcacgataac cacgtcaaga ccataggatt ctcctaattt
    23281 tatgacatct tcttttactg gagaaccatc tccatcaatg aatagcctca ttctttttcg
    23341 ccttcttcca taaatgataa acagttacta atccgctcag taaaagacca acattgatcc
    23401 attttgccaa gataagcagt tgtacacttt ctatcggcac tgattcatgg ttcagcgtga
    23461 tcaataatag atggattggc tctcccattt cttttgctaa aacaaacaaa agcgtcatca
    23521 ataaaaactg gtggatcaca ctccctttat tttgagtagt atacttgaca acgatccggc
    23581 gatccattgg aatagctgta tcagtttgat aatattcctt atttagccag ctgcctgtta
    23641 ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa accatttgga atcaagagga
    23701 ccaataaaga agaactcagt ccaagcaatt gcatagccaa tggagaccat gttaaacgtt
    23761 tcaaaatcca tggtccgact atttttgctg caatcaagcc tgctgcataa ggcaaataca
    23821 taaaaatcga aagtttttct aatccgtaca gtatgccaac ataaaaactg ctaaataaaa
    23881 aaaggaaatt ccctagcatt ccatttagaa aagtaaacag atttagccag cttggcaact
    23941 tccattcctt cttctggtgt cctaaaacaa acacgaacaa gatgaacagc agcaagaaac
    24001 cataaatagc aagatcaaaa agcctttcat cgaataacaa tcgggcattt ctcaaaagaa
    24061 acaataagcc aaagaatatc aaaaataaaa caagctctcg cgtagctacc agatttcttt
    24121 tgatgtcttt gaaatccagt tcgtaacgcg gataatgact aaccgtatgg taggccatga
    24181 cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg agtcggtgca ggcaactcaa
    24241 tcgcttgata caggagaaat agccagatag ctgcaaataa gtactttttc ccatccatat
    24301 tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt attagcaggt acaagccatg
    24361 ctgcactcag ccctagcaaa acacttgaaa aataaaataa aggaaaataa aatacgccta
    24421 atattccaag caatgaacca gcaccaccaa tcagcagagc gatcatcagc aacgtgtaac
    24481 tatctagacc aaatcgtaga ctgttgacta aaaatagtcc agtcatccga aaggcataaa
    24541 acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc tgttagtccc aaaaataaaa
    24601 gataaggaat aaaaataacc gtgttactta agataaaggg cgttgtttgt gtaaaattac
    24661 gcccaaatcg tttgagtttt tcgaacatga caatggctcc ttaaacagtc ttataactaa
    24721 tctgctcttc gattttagca ttaaagggga aataaaccaa ttttacagaa tatattcttg
    24781 taaaaaaaaa tacagaaaga agcataagct tctttctgca tttttacggt acttgattac
    24841 tcaagcagac aagcccttct ccacaaaagt cttgcccgac acccctgcat ccggcaggtg
    24901 ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa ttaggagctc tgtaaaccag
    24961 agtccttctc gacccgtcgc ataaatttac tttaacacag ttcctatgtt tatgcgagag
    25021 cttttttcat tttataatcg accatttgaa atccttgacc gatatctgaa ataagttctt
    25081 ctgtaatctc aaatccttca tgcttataaa cctcgattgc ttgatgattc ccctgattca
    25141 ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag ttcttcatac cagcgaaaaa
    25201 ctgaagaagt caatccttga ccacgtactt tatttagcaa atataattta cttaaataaa
    25261 tttttccatt tatctcttca taagcagtgt aaccgacagt cttttcttcg ctttttaata
    25321 aaaagtagga cacaccttct gtcagttctt tttgaatctg tttttttgac tgataagttt
    25381 tcagcatgta atccacttgt ttttgaccaa tgatcggcac aaaagcttcc ggccatactt
    25441 cttgtatgac tgccatcagc tcatccagtt cattttctgt tacttttatt ttttccatac
    25501 acccacgtcc ttttttacat tataaatgac cttgcgcttg atggaataaa aagagcatga
    25561 tcatgctata cacacccacc gaccatggtt tcaagaaaat cacaatctgc ataaattccc
    25621 taaaagataa tttcgtcaaa ccagctacta gacagatcaa gtcgtctggc gcaaagggaa
    25681 gcagcaaggt aacaactagt acttttttaa tatctttcgt ttttcttgtc agcaattcat
    25741 caaatttctg atactttttt ggcgataaaa taagctggac aaaggctacg ccatagtaac
    25801 ggacaagcaa aaatccgatt gcttccccaa tcactaggcc aatacaacta tataaaagcc
    25861 caaatccatt gccaaacatc agcatgcccg ctactgagga gatccctccc ggcaaaatag
    25921 gaatgatcac ttggatcact tgcaacagaa caaatatcat cactgcatac ttgccaaatt
    25981 gctggataaa cgcttgcaaa gacgtgctcg atcgaaaaat cccatgatga taagcataaa
    26041 gaatcaacac aaagaaaaaa aacagaccaa taatgggcat gatctgaaga catttttgta
    26101 aaactgatcc ttttttagac atctatattc cccacatctt ttttcattta ttaaaactgc
    26161 ctcctt
//
LOCUS       DAYYAO010000035        25609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000035 DAYYAO010000000
VERSION     DAYYAO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.037"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..2232
                     /locus_tag="ACHARI_001372"
     CDS             172..2232
                     /locus_tag="ACHARI_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8442425.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2315..2884
                     /locus_tag="ACHARI_001373"
     CDS             2315..2884
                     /locus_tag="ACHARI_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8442426.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3059..3865)
                     /locus_tag="ACHARI_001374"
     CDS             complement(3059..3865)
                     /locus_tag="ACHARI_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442427.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4003..5517
                     /locus_tag="ACHARI_001375"
     CDS             4003..5517
                     /locus_tag="ACHARI_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8442428.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5920..6903)
                     /locus_tag="ACHARI_001376"
     CDS             complement(5920..6903)
                     /locus_tag="ACHARI_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8442429.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6908..7555)
                     /locus_tag="ACHARI_001377"
     CDS             complement(6908..7555)
                     /locus_tag="ACHARI_001377"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8442430.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7640..8272)
                     /locus_tag="ACHARI_001378"
     CDS             complement(7640..8272)
                     /locus_tag="ACHARI_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8442431.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8288..8839)
                     /gene="coaC"
                     /locus_tag="ACHARI_001379"
     CDS             complement(8288..8839)
                     /gene="coaC"
                     /locus_tag="ACHARI_001379"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8442432.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8836..9573)
                     /locus_tag="ACHARI_001380"
     CDS             complement(8836..9573)
                     /locus_tag="ACHARI_001380"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8442433.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9570..10559)
                     /locus_tag="ACHARI_001381"
     CDS             complement(9570..10559)
                     /locus_tag="ACHARI_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8442434.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10817..11359
                     /locus_tag="ACHARI_001382"
     CDS             10817..11359
                     /locus_tag="ACHARI_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8442435.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11376..12461
                     /locus_tag="ACHARI_001383"
     CDS             11376..12461
                     /locus_tag="ACHARI_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442436.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12473..13279
                     /locus_tag="ACHARI_001384"
     CDS             12473..13279
                     /locus_tag="ACHARI_001384"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442437.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13280..14107
                     /locus_tag="ACHARI_001385"
     CDS             13280..14107
                     /locus_tag="ACHARI_001385"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442438.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14104..15177
                     /locus_tag="ACHARI_001386"
     CDS             14104..15177
                     /locus_tag="ACHARI_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8442439.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15605..18301
                     /locus_tag="ACHARI_001387"
     CDS             15605..18301
                     /locus_tag="ACHARI_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8442440.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18366..18866
                     /locus_tag="ACHARI_001388"
     CDS             18366..18866
                     /locus_tag="ACHARI_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8442441.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18878..19372
                     /locus_tag="ACHARI_001389"
     CDS             18878..19372
                     /locus_tag="ACHARI_001389"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8442442.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19393..20202
                     /locus_tag="ACHARI_001390"
     CDS             19393..20202
                     /locus_tag="ACHARI_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8442443.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20199..21020
                     /locus_tag="ACHARI_001391"
     CDS             20199..21020
                     /locus_tag="ACHARI_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8442444.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21071..23170
                     /locus_tag="ACHARI_001392"
     CDS             21071..23170
                     /locus_tag="ACHARI_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8442445.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23167..24285
                     /locus_tag="ACHARI_001393"
     CDS             23167..24285
                     /locus_tag="ACHARI_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8442446.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24324..25202
                     /locus_tag="ACHARI_001394"
     CDS             24324..25202
                     /locus_tag="ACHARI_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8442447.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25348..25545
                     /locus_tag="ACHARI_001395"
     CDS             25348..25545
                     /locus_tag="ACHARI_001395"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8442448.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8098 a         4454 c         5418 g         7639 t
ORIGIN      
        1 acaaaattaa aaagtacata agttattatt ccgattttct tgaaacaata aaaaatcaaa
       61 aaaaatactc tcctaatagt ccatcttctt ttgattttat ttatataatg aaatcatctt
      121 atgaaaatta ctacttcatg tttagacaag agtagggagg actaagaata aatgtgttca
      181 aaaagacgtt caaaacaact aaataaacac gcttgtcgtt tagataagga acggtcgtgt
      241 tctgaaaaac actacgttaa gtttatgatg cgcacgatgc tgatcagttt atctgtaggt
      301 gtctatatgc atgtacccgt tcaggcaacg acacaaacca tcaaggtcgt acgtacctct
      361 gtttctgaga ccactgattc ccctatcact attactcata ctttcactga tgcaaatgga
      421 aaacagctac ttcctgcatt gacttcctat aataaagtca gttatcctcc aacgataaac
      481 cactactggt taattcctaa caaaacatta tggacatcgg aaaccggaag cagtgtgccg
      541 ctttctatct tttccgatat gactgatcct acaaaaggtg atcagttaaa acagatcact
      601 gatattttga atcatgcttt gacagatata aaggacggtg gaagcgcaca agtttcttat
      661 gtatatgaaa aagattattc acagtttgaa agaaagaaaa tcgatgttct ggcagggaca
      721 aaagtttcga tatctgatct ggtagaaagg ctgaagaacg cagatggaac acaaggagat
      781 atgtccaaag taatttcaaa caatgggaat atcgtggata cgactaatac aggaacatct
      841 actatttcct tgtcctattt tgatccaatt gctttaaaaa caatgactgc atctgctgtg
      901 atccatgttc gtgctgatca aacagctatc aaaggaaaag atcttcactt aagaacagga
      961 gagagttacg cccctaaaga tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga
     1021 aataaagaag aaatcaaaat gaacgggaca gtagatacta caactccggg agaatatccc
     1081 gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt
     1141 tatgtagaag catctgctgc ttcatcgact gtcatcactc attttataga tagtcaaaca
     1201 aataaagaac ttcaagcaga aacacatatg agtagtgtaa atcgtattcc tgatttgcgc
     1261 ctacctgctg aattttctga tcgagtgcca aatatagatt tatcgactta tgaacaaaat
     1321 gggaaagtca cttctatttc tgattggatg caagcaaacg gattagacag aaaaagtcac
     1381 tggtcggaga tccttgaagt acttcactca gaactgatga ataataggaa gcaatcagga
     1441 ggggaaatta cctttactta tgtttatata ccagacaatc gttcattaga aggtcatgat
     1501 cttattgttc atccaaacga aaaaataaac aaagaagagt tgatccaatc agcaacaaat
     1561 tccttaggta aacccgtacc gttaaaggat gtatcggtta caatcaatga agtaccattg
     1621 gaggatggtt atttgaccaa acaaacaggt gattttacga ttatgtatag ttatcatgac
     1681 ccgtattcac tccaagaact ccatgctcag tcactattac atgttttacc attaaacaaa
     1741 cagatagatc aaaaagaaca aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa
     1801 atggctcatg cagtttctcc aacagggaaa gtaaaacaaa caagaagcct agagttagca
     1861 agaatatcta gaaaaatctc agaatctaat aaaacgataa ggcaagaagc tcatttggcc
     1921 ccacaagttt ctggattgga aaatgaaatt ttagagccta aaataaaaca acaatcagaa
     1981 aaagaatttg ttgctaaaca gtcggaagat cagaaggaat cagatgacca gaaggaaacg
     2041 aagaaaaaac gcttgtcaac tattgattcc accgatgagc atctgcagcc gcaagtacca
     2101 cttcctagtc cgggaacacc cgcacctggt ggaggtattc cttcttctgc tttaggagat
     2161 ttcatgcgag gtatggcagg aacatgggta tacgctaatc gaaatgaatt tgacgcagat
     2221 gactggttct gatttatatc tctatctgtt atagtcaaat aagtagctga gtcatatgtt
     2281 atttggcata atggagacga ggagggatag aaaaatgtta gataaaacgt taccttacgc
     2341 agaaatctgg atgacaagac cacttcaaca aaatgttcca atcacgcctt tagcggaagg
     2401 ttttgaattt cgattttatc aaaaagaaga tgaatctgct tgggcggaga ttgagacttc
     2461 cgttggggaa tttcaaacga cagatgaagc acttgagtat tttcaacaaa actttgctcc
     2521 ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt
     2581 agcaacctgt accgcttggc gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg
     2641 gttagcagtg aaaccagatt accaaggaaa agggctcgca aaagcattga ctgccagagt
     2701 cttgcaagaa tttcctaccc tccatacgga cggaccaatc tatcttcaca cacagacatg
     2761 gagccatcag gcgattgctc tttataaaaa aatgggtttt acttttattg ctgaaaatct
     2821 agacggcaca cctaatccag attatgaaaa agttatgcag gttttaagta aattagataa
     2881 ataacgaaat ggtgaaataa gtcctcagta ttgggataaa aaacgaaata gtcattcctc
     2941 atatcttact tttttgagtt aatactatcg gataactaat ggcatgccat acattcaaaa
     3001 aagaaggggc agtgacaaaa gtattgtcac tgccccttct cttgtttttt agcatgtttt
     3061 atcgtttgat ttttttagga ataaaattct tgatgaacgt aaatttttgg taatcgtttc
     3121 gactatagag gtaaccaaat acactgaaaa caactgctcc aataaaatta acaaataaat
     3181 ccttcatagt atccatgatt cccacgtcta agtaaccatt ttggatgatt gttttatctg
     3241 ttttaccatc tgcattttga ctttcaatga ttgtccgagt gatatctgga acatgataga
     3301 cagtgtttcc gtctttactt aactccacac tattgatttg ctggatgatc gtatcttttt
     3361 gcatatcgaa ttgcagtaat ttatctgctg aaaattccac aaactcccac ataacaccaa
     3421 ctgtcatcga aaaacagaag gaaacaactg ctagtaataa aggagtcaag ttcatggttt
     3481 ttgtattttt attgagcaaa taaacaaggg aaaagccgac acccccagct aaaaatccgc
     3541 tgaatgcatg tagaaaagta tcccacattg aaaaatagcc ataaaaatca cttaactctc
     3601 ctaaaactgt tgaactaaaa ataaaagata acaaaatgat ttctagcata tttggaatct
     3661 gtatctttaa tatcttttcc agaaaataag ggatttcaaa taaaattaat gtcagtaata
     3721 aaagcattgt atttccccag tgttggttcc agacctgatc cactagcata ataacgacga
     3781 tgatacgtaa taaaatatag attccgtttg tgattctacc tgcagttttt gtactgttcg
     3841 taaaaaaggt tttcaaatga ttcataaaat aactccttca tgtatatcct taattatact
     3901 aagaagcgtg tgtttaccaa agctatatga cttaaagaaa aacagagttg atattgtgaa
     3961 aaaaatcact taatatagta taaggagaaa agggggatac aaatgaaaaa attagttgct
     4021 ggtttattaa cagccgttgc acttcttgct atgagcgcat gcggcgcaga tcagaaaaat
     4081 acagcgcaga caagttctag tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa
     4141 caagtatata cagatccaaa agaaatgaaa gaatcttatg atgtcgtgat tattggagct
     4201 ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt
     4261 ttggaaaaga tgcctgttgc cggaggaaac acatctaaat catcaagcgg tatgaatgct
     4321 tcagaaacaa aatttcaaaa agaagaagga atcaaagact cgaatgatgc cttctatgaa
     4381 gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa tgcttcgata ctttgtcgat
     4441 cactctgctg atgcggtcga ttggctagat caaaacggta tcaaactgga caacttaacg
     4501 atcactggag ggatgagtga aaagaggaca catcgtccgt ctgatggctc ggctattggt
     4561 ggatacttag tggaggggtt attgaataac gtccatgagc gggaaatccc tatttttgtc
     4621 aacgcagatg tgacagacat taagaaaaat ggggaaacgg tcaatgaagt gactgtacat
     4681 gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt
     4741 tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg atttagctga ttatattacg
     4801 acaaatcaag aaggatcaac tggagatggt atcaccatga ttgaaaaatt aggtggacaa
     4861 accgttgata tggataaaat acaaattcat ccaactgtgc agcaggatga gggggtcttg
     4921 attggtgagg ttgttcgcgg agaaggagca atcttggtcg atcaagaggg gaaaagattt
     4981 gtcaatgaaa tggatacaag ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa
     5041 tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat
     5101 gaccaaaaag gatatgtcac aactggaaag acaatggaag aattagcaga aaaaatcgat
     5161 ttaccaaaag aaacactagc aaaaacagtt gatggatgga atacggatgt tgcggagaaa
     5221 aaagatgctc aattcaaccg tgccacagcc atggaacatg acttatcgtc tccgaattat
     5281 tatgcgatca aaatcgcacc aggaatccat tacacgatgg gcggtgtgaa aatcaatacg
     5341 aacactgaag tattagataa agaaaaccag ccaattaggg ggttatatgc tgctggtgaa
     5401 gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa attcagttgc ggagatcatt
     5461 atttttgggc gacaagcggg catacaagca gctcgatttg cttcggaaaa tgaatgaaat
     5521 agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga gctaataccg agggtcagct
     5581 gattgaacac tgtttagtga agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     5641 cagccaagta aaaacagctt cttatgtttt taacttggtt ggacttatta ccgcagatgc
     5701 aagcttttga acaccgttta ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt
     5761 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag aaattccttc ggaaataagc
     5821 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
     5881 aacgcttctg tcccagcctc ttttttgatc agcatgattt tattcagtta tcttcaaaat
     5941 gatttttccg atgtttttgt ttgcttccat atattcatgc gcttgtttga ctgcgctgaa
     6001 ttggaatgta cggtcaatga ttggccgaat cttgccattt tctagaaaag gcccagcttt
     6061 ttgcataaaa gtttgtgcga gctctgcttt atactgatca cttctaggtg tcaataatgt
     6121 accaagcagt tggacacgtt tcccaatcaa tacaccaagg tctatctcag gcacgatggt
     6181 cccacctagc ataccaatca agacccaacg tccatcaacg gctatagaag ctaaattctt
     6241 ttcccagtaa gaagcgccga tgaaatccaa aataacatcg actccccgac cttcagtgat
     6301 ttttcgtacc tcttcttgaa agtcttgttt tttatagttg atcaattcat ctgcaccaag
     6361 ttctttacaa aaggcgagtt tttcttctga tcctgcagta acgatgattt tcgcgtgaga
     6421 aagctgtcta gctaattgga tcgctgctgt tcccacacca cttgctcctg catggatcaa
     6481 gacagtttcg ccttcttgta attttccaag ccagtaaagt gtttgataag cagtcaaaaa
     6541 aacttctgag attccagcag cagaaacata gtctagcgta tcagggagaa ggattgcacg
     6601 atcggcaggc atcacagcat attcggcata accacctaga ttgactaatc cgtatacttt
     6661 tgtcccagga gtaaaagtgg agatatcact tttattctca actacctctc cagctacttc
     6721 gactcccaaa atagggtagg gaggtttcaa agagactgat tctcttttca taatatctgt
     6781 tcgattgaca gctgctgtat ggactttgat cagtaattcg ccttgttttg gtttaggaac
     6841 aggcacttca atttgttcta atacatctac agaaccagga tgcttgattg aaatggcacg
     6901 catggatcta ttcctccttt gcccattctt ggaatgctgt attcaactct ttgctacctt
     6961 cagaaagtac tagagtcgta cgagaaatat ccgtttcttc cactagagcg gcaagtacag
     7021 cagcaactgt agagcgagtg atcgtttttg atgccttctc attaggctta gttaattgaa
     7081 tggaagtaac ttcttcatcg ttagtcaatg tgactggttg aagaatgaca aaatctaagt
     7141 tagttcgttt cagccattca tctgcataat gtttagtaat ataataatct gtcatggact
     7201 caggccattt tgctggttca tctgcataaa cagcactgac catcagatag cgagagacat
     7261 gcgcattttc agctgcgatc actgttttga ttgcgccatc caagtctact tgcagaagat
     7321 tctttccttg agagccggca gcaaaaatca atacatcgat gcctttgaat gcttcggtca
     7381 ttttttccac ggaccaagtt aaatcaaaag agacatacga aacattctct ttttttacta
     7441 atgtttggga agcaacatct ctcacccctg caaaaatttc atgggaactt ggagctaaat
     7501 cttttatcag gtgtcggcca atttgtccgt ttgcacctac aacaaaaatt ttcatttgga
     7561 tcatcctttc gacgtcaaat ctttccattc aatagtaaca ccaaaagaga aaaaaatcag
     7621 agatcggcta cgatgactat cagttagtaa gagaagggtg cttgaagtat cttaacaaga
     7681 cattgcatac tgctgcagtc gcaagacctg cgacgattcc ttcaggtata ccacttgtaa
     7741 aaataaccgc tagaatagct gaataaacag catttaaatt tgttccgatg actttggcat
     7801 aatcttgctg gaataaaaag tagatcatat tcatcactaa aatcgtattg gtcaatgaac
     7861 cggaaagtcc agctaagaat aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca
     7921 gacctttata aacgaaataa ggaaccaccc caatcaagat gcgaggaata aacgcgatca
     7981 gcaaggcttt ccagcttcca tgttctgttc cgatgactgg gataaacgga gaaaagacaa
     8041 aagacaaagg tgtttggata actgtgctac ggatcatgct gatcagtcca aatgtaccgc
     8101 caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca
     8161 tggttgttgc attgattgga ccgataggga taaaacctaa aggcgtaaca gctaataaaa
     8221 tcaaaatagc taaaaacatc gcagttaatg taaagttttt tgtatttttc ataagtactc
     8281 cttttttcta agcatttctt tcattaagag tcagcatgac ctgttcgatg atcgtctgaa
     8341 tatcagctaa ggcacctttt ccaaaatctc cacaagctaa aagagattct cttggttcaa
     8401 tttcctgata gccgacagat ctcaatgttt ccagattacg ctgagtgatc gggttttgat
     8461 acatattcgt gttcatggca ggggcaatca attttggtgt tcccactgga agcgccattg
     8521 cgactgttga aagaagatcg tctgcaatgc cgtttgctag cttgccgata atgtttgctg
     8581 tagcaggcgc gatgagaaac aagtcagctt gttttgctag ttcgatatga ttgattacct
     8641 gcggatttga ttcttgcatg acgtctgtat ggactgcgcg cttcgacaga gattgcaagg
     8701 ttaatggcgt gataaaatgt gtgctgcttt tagacataat cacatctacc tgggcaccta
     8761 atttgacgaa ttgacttgta agatcagcgc ttttgtaagc agaaatactt ccagtaacgc
     8821 ctagtaagat ctttttcatt ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc
     8881 aatctcagat ttactttgag cttcttccac tgttccatct ttagataaga gatagccgtg
     8941 gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt
     9001 tatcagacta ttttttgcga ctgccagaag agattccttt ggtacatcta ctaatagttt
     9061 gaaaccaatg atttttgctt ttggctgcca ttcacgtaga taagaaatga tttttggtgt
     9121 tttcttcaaa acgagcacaa gatgctcggt gttagaagag atctttgaaa cagtggtatc
     9181 tgtctgttca ttttccgcaa accaattgtc cagttcttct tgcgttgaag aagtgggtag
     9241 gttttttgcc agctgttctt ctgaaaaact aaaagcagga gtaaaatcac tgactgccat
     9301 actgtggata atcgcatcgt agtgttcttt tttcgttagt tcttctaatt gtaaaaataa
     9361 gtccagtgca gattcgatcg tataaatcgt tatattggag tgtcttttcg gtttcagtgc
     9421 ttgtttcgta gtcacgtaat cgattgtggt atccggattg gcagctaaat aatcagctaa
     9481 tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca cgtacttgat cgattttttc
     9541 agtcgttcct ccagctgtga ttagaatctt catttatggt cacctacatt tactagtgtt
     9601 cttccttggt gtgttccagc aagtagttta gatgctgttt caggcaattg atccagagta
     9661 atctcttgga cgaccagttc atctgcaatc tgcaaatccg ccatctgttg ccataaagaa
     9721 agacgttgat ctattggtac attgacagaa tcaattccaa tcaactggat gtttcgcaga
     9781 ataaaaggca agacagtcgc gtttagtttg attcccccag cattgccaca taaagctact
     9841 gcaccgttat aagagatcaa tggaagaaga ctgcttagct gctcgccacc tactgtgtcg
     9901 atgacatagt cgatttgctg ttttcctaaa gcttttgttg tctctggcag aaattcatca
     9961 ggtcgtacga cttgcaaagc acctagactt tttagccatg tgacagactc ttcttttctg
    10021 gaaaaggtaa tgattgactg gaatcctagt ttatgtaaca gggctatcgc tgtactgcct
    10081 acaccgccag aagcaccagt aacgacaact tttttatcgg aggtcatacc ttgacggatt
    10141 aaagcgttca cacacaacat agcagtaaac cctgctgtac caaggatcat cgcttgacgc
    10201 agcgtcatat tttttggtag aggaactaac cagtctcctg gtactttttg atattggcta
    10261 taaccgcctg gatgggtgac gccaagccca aaaccagtag caagtacaag atcttctttt
    10321 gaaaaacgtg gattgttact ttggacgaca gttccggata aatcaatccc tggagtcatc
    10381 ggatatgttc gtatcacctg accagactcg ctcattgcca atgcatcttt ataattgaca
    10441 tcagaatagg caatttttac caagacttca ttttcagcca actcagtaaa tggcagatca
    10501 ctgattgctg tatggaaaac aggttccttt attacctgaa aagatcgaaa agcgttcatt
    10561 cgtgagacac tccttttaaa attttctttt atcataccat aaagcctact cttttattct
    10621 tgttcttccg taaatttaga caaaaattag gtgaaaaata aagaatcaaa caactagaat
    10681 ttattccaaa agataaagaa atattcagca aaaaaaattt tacaaagggt tgcctttttc
    10741 aataaatttc gtatactaat acgagtggta gtaaacagga agtttactat tagaaaactt
    10801 cgattcggag gaaattatgg aaattggtga gaagttaaga aatttgcgca tccaaaagaa
    10861 tttgacacaa gaagaactag gcgaacgcac agatttgtca aaagggtata tttcccagtt
    10921 ggaacgagat ttaagttcac catcaatgga aacattcttt tctattcttg aagtcttagg
    10981 tgtgactcca gaagaatttt ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa
    11041 agaagatcat acggtttatt tcgatgaaga aaacgggtat gaattggaat ggctcgtggc
    11101 tgattcgaat gaaaaagaaa tggagcctgt cttgctgact tttgaaggtg caggagaata
    11161 taagacgttt gaaccttcat tatctgaaac gtttattttt gtattagaag gggaattaga
    11221 gttaagctta ggagagacgg tctatgtggc aaaagcagga cagtcgatct attatcaagc
    11281 gacaaaacaa catcaattaa ggaatcatct caaaaaacga acaaaagtat tgattgttgc
    11341 aacgcaatca tatttgtaat atagggggta aaaacgtggg gaaaccaatc atttcatttg
    11401 atcaagtagt gaaacgctat gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg
    11461 aacaaggaaa attttataca ctgcttggtc cttccggctg tggtaaaaca acgatcctac
    11521 gtattatagc tggattcacc gaagctacag aaggggacat ctattttgaa gggaaacggt
    11581 tgaatgattt gccagctaat aagcgacaag taaatacagt cttccaagat tatgcacttt
    11641 ttcctcatat gaatgttttt gataacgttg cttttggatt gaagatcaag aaaatgtcca
    11701 aatcagatat cgaaaagaag gtaaaagatg ctttgcgtat ggtccagctt cctggatacg
    11761 aaaaacggga gattagtgaa atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag
    11821 caattgtaaa tgaaccgaaa gtattgctat tagatgaacc gttatcggcg cttgatttga
    11881 aactacgtac agatatgcag tatgaactgc gggatctgca acaacgatta ggaatcacgt
    11941 ttatttttgt tactcatgac caagaagaag cgttggcaat gagtgatgag atttttgtca
    12001 tgaataaagg gaaaatcgtc caaagtggta cacccgtaga tatttatgat gagccaatca
    12061 atcatttcgt tgctgatttt gttggggaga gcaatatcgt ggacggtgta atgatcgaag
    12121 ataatctagt tgagtttgtt ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa
    12181 atgaaccagt tgaagttgtt ttacgcccag aggatttgac gatcacgaca ccagataaag
    12241 gaaaactagt cgtaacggtc gatactcagc tgtttcgtgg tgtccattat gaaatcatct
    12301 gttatgatga acagcaaaat gaatggatgg tccattcaac gaaaaaagct aaagaaggaa
    12361 gcaaagtagg attggccttt gagccagaag atatccatgt catgcgtttc aacgagtctg
    12421 aagaagaatt cgatgcacgt cttgacagct acgaagaata ggggggacaa gaatgaaaac
    12481 aatgcgcagg atctattccg tcccttatat tttgtggctc ggtttattcg tgatcgcgcc
    12541 tgtattaatg atcatttatc aatctttttt tgatatgaat gggcaattca cgctgaataa
    12601 ttatctatct tattttacgt cggggacatc gctttctatg acgatcaatt ctgtatggta
    12661 tgcatttttg atcacgtttt tcacgctgat catcagttac ccgactgctt attttttaac
    12721 aaaactgaag cacaagcaat tatggctgat gctgattatt ttgcctacat gggtgaatct
    12781 gctgcttaaa gcttacgcat ttattgggat tttcagtgtg cacggcagcg tgaatcattt
    12841 ctttgatttt atcgggatcg ggccgaaaca gatattattc acagacttca gttttctgtt
    12901 cgtggcaacg tatatcgaga ttccatttat gattatgcca attttcaatg cattggaaga
    12961 gatgaatcct tctttgatca gtgctagtag agatttaggc gcgaatagct ttgagacctt
    13021 ccgacgagtg atttttccgt tatcattaaa tggtgtgaaa agtggtgtcc aagcagtatt
    13081 tattccttct ctttctttat tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac
    13141 tttaggaacg gctatcgaac agcatttctt ggttacacaa aactggggga tgggttctac
    13201 aatcggtgtg attttaatca ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa
    13261 aaaaggaggt cgctcataaa tgaaaaaatt taaatggtcg tatctttatt tagggatcgt
    13321 ctttttattc ttatatgcac cgatcttcta tctgattttt tattccttca atgaaggagg
    13381 aacgatgaac agctttacag gatttacttg ggaaaattat gaagctgttt ttgaagatac
    13441 acgtttgatc attatcgtat tgaatacgtt catgctggct ttcttatctg cattgatcgc
    13501 cacgatcatc gggacatttg gggctatggg aatctattac acgaaaaaac gacgtgcacg
    13561 gacagcgttg ttgagcatga ataacatctt attagtctct cctgatgtta ttatcggtgc
    13621 tagtttcttg attttcttta cactactagg attcggatta ggttttcaga gtgtattgct
    13681 ttcccatatt gctttcagca ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga
    13741 aatgaacgat tcaatggtcg atgcggctag agaccttggt gctaataatt tccaagtagt
    13801 caaaaatatc attttgcctt atctttctcc aggaatcata gctggatatt ttatggcatt
    13861 tacctattct cttgatgatt ttgcggttac atttttcgtc acaggaaatg gctttacgac
    13921 gctctcagta gagatctatt ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct
    13981 aagtgcgtta gttttcttgt tctccatgat attggtcatt ggatactact ttattagtaa
    14041 agaaaaccgt ccaaaacggc tgaagaaaaa cagaagagta cgcgaggggg tggcacggct
    14101 gcgatgaaaa aattacaatc tctgttttta ggtattctca tcattattgc ggtgctgctg
    14161 cttggtgttc gtcaacttga aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc
    14221 tataactggg gagattatat cgatcctagt ttgatcacga aatttgaaaa agaatatgga
    14281 tacaaagtga attatgaaac atttgattct aatgaagcga tgttcacaaa aatccagcaa
    14341 ggtgggacaa actacgacat cgctatacca agtgaatata tgatccaaaa aatgatcaaa
    14401 gaaaaactag tacttccatt agatcattcg aagatcaaag gattaaataa tattgatcca
    14461 cgatttttag acttggattt tgatccagat aatcagtatt cgattccata tttttgggga
    14521 actttaggga tcgtatataa cgataaattc tttgatgcaa atcaaattaa gcattgggat
    14581 gatttgtgga agccggaatt aaaagacagt ttgatgctga ttgacggtgc acgagaagtt
    14641 atggggcttt ctttgaatag tttaggctat tctttgaata gtaagaatat ggcacagctg
    14701 acagaagcag caaataaatt gaatcattta acaccaaatg tcaaagcaat cgtggcagat
    14761 gagatcaaaa tgtatatgat caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa
    14821 gctgccgata tgatgtggga aaatgaacat ttacattatg tgatcccacc agaaggatcc
    14881 aatctttggt ttgataatat cgttatgcca aaaaccgtga aaaataaaga aggtgcctat
    14941 gatttcatca actttatgct agaaccggaa aatgcggcac aaaatgcaga gtatattggt
    15001 tattctacac caaatgaaga agcaaaagcg ttattgccaa aagacatttc gtcggacgaa
    15061 caattctatc catctgatga cacgatctcc catcttgaag tctacaaaga tctaggatcg
    15121 aaatacctag gcatctataa tgacctattc ttagaattca agatgtatag gagataatgg
    15181 caaaactcag taagaatggg gacttatcaa cgttctaaag agctaaaaat cgtccttttt
    15241 ctatctgagt tttccaatct tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat
    15301 tttatttttt ctaccacgtt tttctaccag agaaaagtgg acaaaaccat tgtaaaatag
    15361 ggcccgagac gggctagtaa atctaagatt gaaaaagtta cagaaggata aagtaattgg
    15421 aaactatgca aatatactta acccttctgt aatctattaa taaaactaat atatgaatcc
    15481 agttcaatat ttgtagctat gaaaaaacat aaaaatcata ttagataaaa actaaattgg
    15541 tagataaatt attttgtcag atatctgagg caaatattcg actatttgtg tttcaaagta
    15601 aaaattggaa tacaaatata gaatagacat attgcagccg atagtttatt atgcttttag
    15661 acacattaaa ctggaggaaa aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct
    15721 gatacgacta acagggaata tgtcttctgt cactgccgag aaaatttcga agaatctagg
    15781 tatctctcgt cagaatgcca gccattattt gacgagatta gtggaagata aaaaggtcga
    15841 gaaattaccg ggaaagcctg ttttgtggaa gcctttagat gaatatgcgg ttgtagataa
    15901 tacggagcaa ataaacgaag cctttcattc tgtagttggt catgacggaa gtctgagaga
    15961 ggtcattcaa aaatgtatag ccgctgtcaa atatccgcct aacggactca gtgtattaat
    16021 aaatggagcc acgggtgttg gaaaaagttt tttagccaca aaaatttttg aatacgctat
    16081 ccatgagcaa attattgaaa aagatgcacc atttgctatc ttaaactgtg ccgattatgc
    16141 ggacaatccc gaactgcttt cggcaacact gtttggatac aagaaggggg cctttacggg
    16201 ggctgaaaag gataccgatg gtttattggc aacggccaac aacggctatc tttttctcga
    16261 tgaggtccat cgcctatcga aagaaaatca agaaaagctc tttctattta ttgatactgg
    16321 aaactatcgt ccagtcggtg aaaatgtaaa ttggcattct gctaaggttc gttttatttt
    16381 cgccacgacg gaaaaaggtg aaaattattt attagacacc tttgatcggc gtattcaaat
    16441 tagtgttatg ttgccaactt ttgaagatcg ccccattcga gaacgattag agctgattca
    16501 attatttttt caaaatgaag cggatgtttt acaaaaagat attattgttt caagagaagc
    16561 cttgtcttta atgttatctc atccctttag tgggaatatt ggtaagctga agaatatcat
    16621 taaaatcagt tgtgcggatg tgtatagtcg aacacaagaa gaaccattaa cgattgggaa
    16681 aaatgagatc ttgattcagt taaatatgct ggaatccgaa gtagaaagct tgccgattgc
    16741 tagtttattg ataaagaaaa atcaaaagat aaaaacggat cgaatcaaac cgtttgatta
    16801 tgattcgatt ttggaaaaaa ttactcagct gataaatgaa ggtaatttca atgaaaattt
    16861 ttcttcgata aagaatgaag tacagcattt ggtgcatcac attgataagg attctttttt
    16921 atcaatgagt agaaaattga tgattcagat gtttagaaag gtctgggtat ccgttttagg
    16981 aaaaacgtac ggattgacga cgagtggacc tgttgcagaa attattagca gatcctatgt
    17041 catttcccca agctataaga cagacttaac cgggataaat cgtacattag ctgcaaatct
    17101 acctcggacg acttacttat gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa
    17161 ggacgagaac tttctaagac tgcttattgt cgtgacttta tctgattatg tagatgaatc
    17221 catcgagctg aagggcctgt tgcttgctca cggagatagt acggctagca gcattcaagc
    17281 agtagtgaat caattatgcg gcaattatgt ttttgaagcg ttggatatgc cagtagagag
    17341 taatgtgtca gagattgtcg aatcgacaag gaatttcgtg aaacggcaag tgaatacaga
    17401 tagtctagtg ctgatcgtag atatgggctc tttaaatcaa atttacacac agatcaagaa
    17461 cgatttaaaa ggggagttac ttcttctgaa taatttgacg acttccattg cacttgatat
    17521 tggcttgaag atgacgatga atgcgccatt taaacagatt gcggaggatg cagcagacaa
    17581 gtattcaatc aatgttcaat actttgaagg cttttctcaa agtagcaaca ttatcatttc
    17641 ctgtatgtcg ggtttgggga tttcggacaa attgaaagat gtttttcgtc atcatttatc
    17701 ggatgaatcc atcgaagttt tcacgaagga ctacagagaa ctacgtgaat taatcgacca
    17761 aaatgatgaa acttattttg acaagacaaa attggtcatc acgacttcgg atcttccacg
    17821 ttccttttca attcctaata ttaatgttta tgacatctta gacggtgatg gagcagccaa
    17881 tttggagcat attttgagtc ctgaaatgaa gccggcagaa tttaaccgct tgattcagga
    17941 gctagtaaaa ttcttttcgt tggaaggaat tgcggaccgt ttgaactttc tgaacccaac
    18001 gatcgtgatg agtgaagtgg aaacagtcat ttctaaatat gaaaattact atcatttcac
    18061 tttaaatggc cgtgtaaaac taaatctcta tatgcatacg gcattgatga tggaacgttt
    18121 attcttggct cggacagaaa aatctgaaga accgaattat gatttgaaac cagatgaaga
    18181 tgaattttat tatgtttcta gaagtatttt tcaatcaatg gagatgaaat ataattttaa
    18241 agtcaatgct tatgagcttt cgctgctcta tgaattatta ggaccgtata ttcaaaaata
    18301 aagtgtctaa aaaagtgtct aatctgtttt ttttaggtta gacacttttt ttaatgtgaa
    18361 ttaacgtgat taaaccaatg tttttaaaag ttggcacagg aattgcttat ataaaagtga
    18421 acagaaagaa aggaattaac catatgaaga aaattctaat agcgactcac ggtcacttag
    18481 cttcaggctt tcttagcagt attcaattac tgacggggaa ggttcaagag attacggtga
    18541 ttaatgctta tgtggatgag tgtgattttg aacaggagtt gaatgtattt gtaaaccagg
    18601 tgaacgagca ggatcagatt tttgttttta ccgatttatt tggcggaagt gtcaatcaga
    18661 agattaccaa aacctttttg gagaaagaga ttgctgtaac tattttgtct ggctttaatc
    18721 tgccaattct gctcgaaata gtattggcaa ccaaggaatt aacaaaggag gatattcagg
    18781 aactggtaaa taaatgccaa caggaattaa agatgaccat tcttgatgag gtacatgtag
    18841 taaatgaaga ggaagatttt ttttaggagg aagaaaaatg attaaagcag tgcgaattga
    18901 tgaacgatta attcatggac aagtagcgat gacttggaca aagagtctaa atttaaccgg
    18961 gttagtagta gcaagcgatg aggcggctac caacgatatt caaaagatga cattgaagat
    19021 ggctgcacca tcgaatgtca aagtcattat taaaacagtt gatgacgcga ttcatttgtt
    19081 aaaagatccg cgggcgaagg acatgcgctt gatggtttta gtccctaatg tcaaagatgc
    19141 ggtaaaagtt gcgaaagctt tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg
    19201 gaaaatgaca ccgccatcgg aagataagaa aaccttatca aaggaagtga tgttaacgac
    19261 gtcagaaatt caagacttaa aagaattagt tgaactttat ccagataccg tttttcagcc
    19321 tacaccggcg atggaaaaac gttcagctat ctctattttg agtgattttt agaaaaagga
    19381 aggagaaaaa ttatgtatga tgcatttgta gtcgctttgg ccgtttttat tggagttgct
    19441 ggacatgaaa tgttcgggat gtccatgctg agtcgtccga ttgttgttgc tccgttagca
    19501 ggagcgttga tgggggattt gcagacgggg ttgattgtcg gggcgtcact agaagccatc
    19561 tttatgggtg tcgtcaatat cggaatctct agtacggcgg agccttcttt ggctgctggg
    19621 ctggctacag cgttcgcaat caaaatggga ggaaacgtgg atgcgattat tcccattgtc
    19681 tttccactag cagtattagg attacagtta atgaatatga ttttttcttt tgtatgtgga
    19741 ccgatggctg ctaaattttt caaatttgca aaatctggtg atcaaaaagg aatcatcaat
    19801 ctgcacttct ttatgtggtt tgtccactat gcgctttatg ctcttattcc attctttgcc
    19861 gttttatttg gagcagatgt tgtcaaaagc gtcttggata atattccaga ggtcatcatg
    19921 aacggattaa cagtcgctgg aaatctgctg ccggcagttg ggatggcgat gctgttacgg
    19981 atgctttgga gtaaaaatat tgctgtgtgg tttttctttg gggccgttgt tatggcgtat
    20041 ctcgagttac cattgattgc aattgccgca gttggaacga ttattgcaat tgttatagcc
    20101 caaagagatc tacaactaaa aaatatatct gttcatgcag ttcaaagcga tgctgctgta
    20161 aatgcaacgc ttgaccaaga agaggaggat ttctttcaat gaaattagct gatggaattt
    20221 ctaaagatga gaaaaaattg gttcggaaga tgttttggcg ctcgatgact atgtatgctt
    20281 cggtgaatcc ggtgacgatg ggcggcggtg gtttcgccta ttctatgatg cccttcatta
    20341 atcatttcta caaaagtgaa gaagatcgag ctgcggcgct ggaacgtcat acacagtatt
    20401 tttcgacgac cattccttgc gcgtcctttg tcatggggat tgcggcatcg atggagaagg
    20461 aaaattctga aaaagctggt tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga
    20521 tgggaccatt ggctggaatt ggcgattcat tgttctgggg agtttggcgt gtcgttaccg
    20581 cgggactagc gatcaactta gccaatgccg gaaatatttt ggcaccaatt atctttttag
    20641 ttatgttcaa tatacctaat tacttgatgc gatactatgg cggcgtttta ggctattcat
    20701 taggctctaa atatattgaa aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag
    20761 ctgctagtat cttgggtctg atcatggtag gtgcaatgac gatcagtatg gtcaaattca
    20821 ataccgtttt gagcttttca atgaagggcg aagaactaat gaatgtccaa gaaatcctcg
    20881 atagtatttt taaaggaatt gtaccgttat tggtaacatt gagctgtttc aaattactag
    20941 ataaaaaagt cggcgtcatc aaaattattt ttggaatggt cgtcttggga attttattgt
    21001 cattacttgg aatcgcatag taggaagctg cagctactag gctgcagttt ttttagcaag
    21061 gagggaaaag atgactatta atgaagtaat ttattcaccc aaacacccat tttttaatgc
    21121 tgagttaaac gacggaaatg tgactacttc aaacgaatac agctactatc ggaatattcc
    21181 tgatgtaaag ggattgacct tcgatgtaag taatcaaaag acattagcaa accttgatgt
    21241 ttgcgggacg attcaaacgt tgacctttta tcgagaaaat ttactaactg aggaaaaacc
    21301 aggtgtttgg gtcaataaac agctctctca aaccaattcg ttatccttaa aggttgaagt
    21361 aaacgggatt gtctaccatt tgtcagaatc ggatcatcgt attgaagtcg atgtgattca
    21421 agactgtttg cctagatata ttcatcatta ttcgacgttt aaggtcattg tagtatcctt
    21481 tgctcccata ctggctaata agcgtttaag tatgcttgtt caacaggttt acattgtgaa
    21541 tgaaacacac gaaagccttc aagtttcagt gaaagatgtg ccttcgtatc aagagaagta
    21601 ttcggatcag caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca
    21661 ggaagtggca agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac
    21721 ttttcaggaa tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct
    21781 atttgggcag ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta
    21841 tcaaagcttt tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg
    21901 cagctatcct gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt
    21961 caccatgttt gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt
    22021 taagtttcct ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc
    22081 cagtgcgtta ctggctagtc tttattttga acatactggg gatacaaaat tctttgagcg
    22141 gtgtccgcag gttctactga atgcttcaag aattattgag gacatattag ctcaacgtcg
    22201 gtcaggggaa ccaatgcttt ttccatctgt ttggttatca gacgcctttg cacttggaaa
    22261 gtatcatacc ggcagcaatc tttgtctttg gaaggcctgt attggacttg cagaaatttt
    22321 tgaagcccta gaacaactga gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa
    22381 ggaatcaatt tgtaaacaaa tgactatcga tggtcctttt ggtgagcaat ttttggaggg
    22441 aatcggtgat gaggagaaaa caacctattc agtgaagcac tatcaaaaac cgattcttga
    22501 gcaagggtta atctttttaa gtgatgtgat tgtggatgga aaaattaatt tattgatgca
    22561 tgatggagaa gaatcagaca ctacgttgat gcctttttat caatttttag ataataaaga
    22621 tcatttatat cagaacacca tggccttttc tgctagttct ttcaatccaa cctatagcga
    22681 ggaaatcaaa ggcatcactt ggggattaga atccggcgcc acttttcctg gctttatcac
    22741 tgttttaatg agcgagctgc acaaccctca agctttcgta acccgattag aagaactaat
    22801 ttgtttggca gatttagacg gttcgtggtg gtggtggccg tatcgcttag aggctcaaca
    22861 aggagaagtc gtgcgagact ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt
    22921 cagcctaatg atcagtcaat atttcggatt gaaattcaaa aaaggagtat tgcaaattga
    22981 tcctgttgat aatttaacgt tttcttggaa gaatttgaag ttcggtcaag ccttcatttc
    23041 gattgaatgt acgcaatccg agttgagtat ttcaaatttg tcagaaaagc cattgaaaat
    23101 aagtgatacg aaaaaaattc ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag
    23161 aaaaagatga gtatactaaa aaatattacc caatttaaaa aaatccagag tcgagcaatc
    23221 actcccgaaa atccaacagg cgagaaaggt aaagctgcga tgttgccaag cgagctagga
    23281 ccaagccgca aaggtcgtgg cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg
    23341 gatattactg gaacgggaat aattcgtcat atgtggatga cgattcgtga tcgaacagaa
    23401 tttggcagtt ttgttttacg tgatttaatc ttaagaattt attgggatgg gtcggatagc
    23461 cctgctgttg aaagtccttt aggggatttc ttttgtaatg ggtttggaga gcgttgcgag
    23521 gtgaactcgt tgccgatagt ggttaatcct acagggggct ttaattccta tttcgaaatg
    23581 cctttccaaa catccgctaa aattacgatc gagaatttac atccgaagga tatgtcctca
    23641 tttttttata cgataaatta cgctttggtg gacgaattgg aagaaaatag cctatatttt
    23701 cacgcaaatt ggcatcggga acgcattacc aaattacagg aggattataa gttaattgat
    23761 cgattaaagg gtcaaggcta ttacgtgggt acttaccttg cgcttacctg cctagaacgt
    23821 tattggtggg gcgaaggcga gtttaaattc tacattgatg gggatagtga gtatccgacc
    23881 gtaacctcaa caggttcaga agattatttt ggcggggcgt gggcctttca tgagtacgat
    23941 gagttcggac gtccacatgc gaaaaactat tcaacagcct ttttagggta tccttaccaa
    24001 tcaaatagag atgccacaag agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc
    24061 tttggtgacg acgcaatgcc acgacatggt ctttatcgtt ggcatcttca agaccccatc
    24121 tgttttaccg aagatttatc ggtcacttta caacaaatcg gcaacgatga tgttcgctta
    24181 tttgagcggc aagacgatgt tgctagtgtc gcttactggt attcaacaga agcttcagga
    24241 aatactaaac gtcatttcac tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac
    24301 atattcgaag ggaggaacct atgatgatat atggggcaat tgaagctgga gggactaaat
    24361 ttgtctgtgc agtcggtact gaggaattaa cgattttgga aagagtcagt tttcccacaa
    24421 caactccagt tgaaacaatg aagcaggtca ttgatttttt taaacctttt aaggaacaat
    24481 tggcaggcat cggggtcggc tgctttggtc caattgatat tcaatgcaaa tcaacgagtt
    24541 tcggtcatat cacatcaaca ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc
    24601 taaagcaata tttcaatatt ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg
    24661 gtgaatatgt tgctggttct ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa
    24721 caggtatcgg aggtggtgcc ttgttcaatg ggactttcgt ggaagggttc agtcatccgg
    24781 aaatggggca tatgttgatc aagcgacatc caaaggacaa tttttcagga aactgtccct
    24841 tccaccaaga ttgtttagag gggatggcag caggaccagc gattgaaaaa cggttgggtg
    24901 taaaaggcca gaatttattg gctgacgatt cgttttggca gattgaagcc ttttaccttg
    24961 cccaatgtgc atacaataca acactgatgt tttcgccaga tagaattatt tttggcggtg
    25021 gtgtaatgaa acaagaacac atgaagaaaa aagttcagga taaatttgtt gagcttataa
    25081 atggatatgt tgaaatacca ccaattgata gctacattat tacgcccgaa ttaggtgata
    25141 atgccggaat aatcggcgga ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat
    25201 aattaatgaa tgtttcattg atactcatgt caaaagtatt taaccgagta atcaaatgat
    25261 caaatgaagt tggataaagc gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg
    25321 ttgttttcat taaatcactg aaaggaaatg cttctgcgtt tccttttttc tttttgttgt
    25381 gcgctgtata ccttgaggat attcactggt ggcgtacact taaatagtaa tatattgtgt
    25441 tactttatgt ctgtggtaat agttatagtt gcttaccaat gtatgttcat ttttcgaaat
    25501 gttgacttca taatccttgg cttaatgttt gaattttttt ggtgatagat tttttcagcc
    25561 gtataaaata aatcgtaatt ctattataga gagttgtgat tataaaaag
//
LOCUS       DAYYAO010000036        25565 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000036 DAYYAO010000000
VERSION     DAYYAO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.038"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..278
                     /locus_tag="ACHARI_001396"
                     /pseudo
     CDS             150..278
                     /locus_tag="ACHARI_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            392..631
                     /locus_tag="ACHARI_001397"
     CDS             392..631
                     /locus_tag="ACHARI_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442449.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            743..937
                     /locus_tag="ACHARI_001398"
     CDS             743..937
                     /locus_tag="ACHARI_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442450.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1023..1694
                     /locus_tag="ACHARI_001399"
     CDS             1023..1694
                     /locus_tag="ACHARI_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8442451.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1747..3723
                     /locus_tag="ACHARI_001400"
     CDS             1747..3723
                     /locus_tag="ACHARI_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8442452.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3738..4490
                     /locus_tag="ACHARI_001401"
     CDS             3738..4490
                     /locus_tag="ACHARI_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8442453.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4506..5261
                     /locus_tag="ACHARI_001402"
     CDS             4506..5261
                     /locus_tag="ACHARI_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8442454.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5274..5534
                     /locus_tag="ACHARI_001403"
     CDS             5274..5534
                     /locus_tag="ACHARI_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442455.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5531..7621
                     /locus_tag="ACHARI_001404"
     CDS             5531..7621
                     /locus_tag="ACHARI_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFM8442456.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7648..8112
                     /locus_tag="ACHARI_001405"
     CDS             7648..8112
                     /locus_tag="ACHARI_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442457.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8109..8351
                     /locus_tag="ACHARI_001406"
     CDS             8109..8351
                     /locus_tag="ACHARI_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFM8442458.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8369..8671
                     /locus_tag="ACHARI_001407"
     CDS             8369..8671
                     /locus_tag="ACHARI_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442459.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8741..10804
                     /locus_tag="ACHARI_001408"
     CDS             8741..10804
                     /locus_tag="ACHARI_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442460.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10804..13416
                     /locus_tag="ACHARI_001409"
     CDS             10804..13416
                     /locus_tag="ACHARI_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8442461.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13413..15944
                     /locus_tag="ACHARI_001410"
     CDS             13413..15944
                     /locus_tag="ACHARI_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8442462.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15994..16326
                     /locus_tag="ACHARI_001411"
     CDS             15994..16326
                     /locus_tag="ACHARI_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442463.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16327..16920
                     /locus_tag="ACHARI_001412"
     CDS             16327..16920
                     /locus_tag="ACHARI_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442464.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17083..19107
                     /locus_tag="ACHARI_001413"
     CDS             17083..19107
                     /locus_tag="ACHARI_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFM8442465.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            19107..20306
                     /locus_tag="ACHARI_001414"
     CDS             19107..20306
                     /locus_tag="ACHARI_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8442466.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            20322..20966
                     /locus_tag="ACHARI_001415"
     CDS             20322..20966
                     /locus_tag="ACHARI_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442467.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20977..21783
                     /locus_tag="ACHARI_001416"
     CDS             20977..21783
                     /locus_tag="ACHARI_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442468.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21805..22035
                     /locus_tag="ACHARI_001417"
     CDS             21805..22035
                     /locus_tag="ACHARI_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442469.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22549..22857
                     /locus_tag="ACHARI_001418"
     CDS             22549..22857
                     /locus_tag="ACHARI_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442470.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22859..23281
                     /locus_tag="ACHARI_001419"
     CDS             22859..23281
                     /locus_tag="ACHARI_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442471.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23301..24890
                     /gene="mobP2"
                     /locus_tag="ACHARI_001420"
     CDS             23301..24890
                     /gene="mobP2"
                     /locus_tag="ACHARI_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFM8442472.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24902..25237
                     /locus_tag="ACHARI_001421"
     CDS             24902..25237
                     /locus_tag="ACHARI_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442473.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25378..>25565
                     /locus_tag="ACHARI_001422"
     CDS             25378..>25565
                     /locus_tag="ACHARI_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442474.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9541 a         3961 c         5256 g         6807 t
ORIGIN      
        1 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
       61 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      121 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aatgacgctt acgaatgata
      181 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      241 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      301 agataaaggc ttagaagaag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      361 gagtaaggaa ttcatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      421 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
      481 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
      541 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
      601 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
      661 tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa
      721 cagaaaaaaa gtgtcgaaat aaatggaaga aaagttgcta aaaacgatga aacaaaaaca
      781 ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag gaaaagagaa
      841 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaac
      901 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
      961 taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa gggggtagct
     1021 ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     1081 ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa
     1141 agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat gcaacgaacg
     1201 tcaataatga tatgtctttg gggcaaatgt tgtccataca acaagaagca atcgaaatgg
     1261 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccaa
     1321 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
     1381 atgctgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
     1441 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     1501 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgagtac tttaaattaa
     1561 tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga gagaaaccct
     1621 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1681 gagaactaca ataacaaggt tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag
     1741 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttattgg aatgttagga
     1801 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     1861 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1921 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1981 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     2041 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     2101 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     2161 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     2221 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     2281 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     2341 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     2401 tttggtgacg atggcgcaga ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     2461 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     2521 ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga agttccttcg
     2581 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2641 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     2701 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     2761 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     2821 catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg tgtgaaaagt
     2881 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaggg actttcttat
     2941 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
     3001 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
     3061 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     3121 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     3181 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     3241 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
     3301 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
     3361 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
     3421 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
     3481 ggtgaatatt cactggaaga aacaaaagcg cctgaaaact atcaaaaatt aacgaaagag
     3541 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaac
     3601 aatcaaaaag cttccgtacc aatgaccggt agcaacggtt ttcaaacgta tgtattaatc
     3661 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
     3721 tagaaggatc aaaaccaatg attatcagga agcctaagaa aaagcgaatc atgggtaaat
     3781 ggatcattgc tttctggtta ctttcagctg taggtgtctt acttttgatg cctgctgaag
     3841 cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg
     3901 cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta
     3961 ttcttacaat tctaagtatt tctttaaaac taccaattta tgacggaacg tccgacaaaa
     4021 ttttagaaaa tggtgtaggt attacggaag gaactggtga tattacagga ggaaacggta
     4081 aaaatccttt aatcgcaggt catagtggac tttataaaga taatcttttt gatgatttac
     4141 cttcagtaaa aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc
     4201 aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact
     4261 tagaatcaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca
     4321 acacacaccg ttttctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag
     4381 atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc
     4441 tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa
     4501 ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg
     4561 cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa
     4621 tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag
     4681 ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc
     4741 acgttcaaaa gattatttct tcagaacaat tagacaacca ggggcatgaa aaacaagaag
     4801 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
     4861 ttaaggatct cactactgac caaattgaat cccaattaaa aaatcgagtg aaaaaactat
     4921 ccagtgatca attgaagcta gtttctaatg aagaaacaaa gacaattgat caacaaacag
     4981 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
     5041 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
     5101 ctgataaaaa cggtgaattc ttaaaggatg tgtggatcta tcctaaatca gaagcttcgc
     5161 aacctaaaga agaagtaaaa aaaatcgttt ctactggcgt aaagaaaaac ttctttgaga
     5221 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
     5281 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
     5341 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
     5401 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
     5461 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
     5521 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
     5581 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
     5641 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
     5701 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
     5761 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
     5821 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
     5881 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
     5941 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
     6001 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
     6061 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
     6121 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
     6181 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
     6241 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
     6301 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
     6361 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
     6421 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
     6481 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
     6541 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
     6601 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
     6661 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
     6721 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
     6781 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
     6841 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
     6901 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
     6961 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
     7021 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
     7081 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
     7141 acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac aatttagtat
     7201 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
     7261 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
     7321 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
     7381 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
     7441 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
     7501 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
     7561 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
     7621 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
     7681 attgccatga ttgttatttt agtcggatgt agtaatgaaa aaaataaaca aactaatcaa
     7741 gataatggcg tattgaaatc aggaactatg tggaaagaag aagtaggtgg tcttgtttat
     7801 aatttgaaaa ttatagacga gactacctgg gaatactctg agagtgtatg gcatccagat
     7861 cctgtacaaa taacagtcaa gaggcaaaaa gactataaag gtttagaaag atacaaaata
     7921 gtagattcag ctggtgttag ggaatttata aacaaatctg attctttatt tattgttgtt
     7981 ccctatgaga agaatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgaaaag
     8041 cgaacgaaag agaaattgat tcacgatgga tcacaatcca ataaatataa gttacaaaag
     8101 actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca gtggcaaaag
     8161 ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt gtatctgaaa
     8221 cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg ggaaaagaca
     8281 aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta
     8341 ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga attttttaac
     8401 acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg ccgcaactag
     8461 tttaaaaaac aatctgatga tttttgctgc tgctgcggcc gttgcttcca ttgttattgg
     8521 gggtcttttc ttcctatttg gacgaagagg agctgaaaat gggaagatgc acgtaggcta
     8581 tgtattgatg ggggtaggag ttattttagc agcagcttct attgtggttt cgttatttgg
     8641 attgtttgga caaacacctc aaggattata agaaagtaaa aaagtaggag taagaagtag
     8701 tcgatacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgattgcagg
     8761 caataccaaa acagataaag agttaataaa aaaagcaacg aaggaagtag gaaagcgtac
     8821 ttctttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
     8881 gttagggaaa gtactggaac acgaaagtcg atcttctgtt aagttgactc tcacgttaca
     8941 tgacacagaa ggaaaaatag agaaagctgg tacagaatac gtttttggcc cactggatta
     9001 tcaaatttca gaaggtttct atcctacatt gatggattta atccgccttg atgtgtttaa
     9061 agaaacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt
     9121 tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagggagtg aagtcgaagt
     9181 aaatgaagaa gtaccatggg aacgaccaat atttccaaag aaggagtcaa aagaaaaaga
     9241 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
     9301 gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
     9361 gacggccgaa gtagaagttg atccagttga agcgaatgaa gagtcaacat ggactgaagg
     9421 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaataga
     9481 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
     9541 gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca
     9601 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
     9661 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
     9721 attagccacc tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
     9781 ggaacagtcg tctaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
     9841 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
     9901 ttccttgtgt gtagcaaaga tcaataattt tactcagacg aatcaagaag cgttatcgaa
     9961 agaaaaagaa aacttgttaa aagaacgtat taaagaacga aaagaagaat tttaccgagg
    10021 tttgcgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataagctata
    10081 tcaaacaacg actgaagttc ttatggaaaa atacgaaaag atccagcaag aattacaagt
    10141 gcaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
    10201 agaaaaacaa cggcaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
    10261 ggatcttgcg gaacaacaaa tggcaataga aaaacaacgg ttaaaccaag aaaaggaaaa
    10321 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagctcagtt
    10381 aaattatcta gcacaacctc aaccaacgcc ttcagtagct gctgttcaaa ccccaatgaa
    10441 caaccaaaca aatctaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
    10501 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    10561 tcaatcttca caggctgatt ctgtggccac aacgacgtca atcaaggaag aagcccaaga
    10621 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    10681 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    10741 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    10801 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    10861 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    10921 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    10981 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    11041 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    11101 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    11161 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    11221 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    11281 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    11341 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    11401 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    11461 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    11521 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    11581 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    11641 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    11701 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    11761 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    11821 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    11881 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    11941 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    12001 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    12061 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    12121 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    12181 ggctataaag gattttgtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    12241 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    12301 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    12361 ccagaattag aaaaagaagc cgttctaaca ttagatgagg agaaaagtaa cttattagta
    12421 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    12481 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    12541 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    12601 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    12661 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    12721 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    12781 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    12841 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    12901 gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    12961 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    13021 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    13081 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    13141 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    13201 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    13261 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    13321 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    13381 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    13441 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    13501 aagtcttaca agattatggt aattatctgc agtatcaaga tgtatttagt tggatttgga
    13561 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    13621 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    13681 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    13741 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    13801 ttatggccat gatcttgatt tttggtggga gttctttaat cgatgaaggg ttaaatatca
    13861 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggttcacctg
    13921 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac attactagca actgacccga
    13981 ataaagtaga atccctgcca acagacaaaa gaaatgcttt aacagcgaaa aattttaaaa
    14041 cagcgaatat caatgggatt attactccta aacaagctaa agaaatggcg gataaagata
    14101 agagtggttt atcagattta caaaaggatc ggttaagagc gttacaatac cgattggaac
    14161 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    14221 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatctta gctggtgaat
    14281 cttctttggc ggtggcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    14341 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggccaac
    14401 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    14461 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    14521 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    14581 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagtaatt
    14641 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    14701 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    14761 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    14821 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    14881 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    14941 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    15001 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    15061 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtt aatgcagcag
    15121 taaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    15181 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    15241 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    15301 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    15361 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    15421 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    15481 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    15541 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    15601 atagtcgttt gaatcaattc aaggagaatg atcaaagctt tgttcaccaa tcaactgcag
    15661 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    15721 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    15781 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    15841 ttactgcacc aacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgcattga
    15901 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    15961 gccttttatt tatatgaaaa ggagaaatca attatggata aatatataaa catgaaaaac
    16021 cctccaagat ttagtggaga atggaagttt ttcgcttttt tctttttaga tctattaacg
    16081 cttggtggct gctttttttg ggcgaaaatg gtcaataatc ttgtccactt accactcttt
    16141 tttgcactct tgaattatgg cttatggatt gcgatagcgt tagtgattgt ttggcgaccc
    16201 tataataacc caggtcaacg acaaattttt gcgattatcg agtctatttt ttttgcggac
    16261 aagaaccatt atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
    16321 gaataaatgc gagtcaaagg acaaaattca gttgaaagga aatccatttt gcagtttgga
    16381 aaaccagaag agcgctctat cttggactta tttccgtata aatcctgtac agaagaggga
    16441 gtcttaatta ctagagaaga tcattttcaa cgttattatc gtgtagtttc tacagacgtg
    16501 gaaggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
    16561 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
    16621 atcgtgcaaa agcggcaatt attagataaa aatcgtatgg aacagtcctt aggaagaaat
    16681 ctacaacgat taaaaaaata tgaaaagaaa ttagtcgaag aaatccagga attgaaacga
    16741 gcagaacaag aacgtcctga cttaagcttc tttttcgtca ttgaaggaaa gacaatcaag
    16801 gatttacaaa ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca
    16861 ctctacaaaa aagaattgat caagattctg tatcgaatga acaatatgaa tgatgagtag
    16921 gaatggagaa gagagaatga aatatccgtt gatatggtta gaaaaagatg ttccgaagga
    16981 agagaccact agaaaatttc ctcacacttt aagcctgggt aagttgggaa aagggcaatc
    17041 ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcaattaaa
    17101 acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca actaggaaat
    17161 cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa acaagtgatg
    17221 ccacaaggtg ggttaaattt tgatgaagaa tatgtgacgt atggtaatgg ttattcacga
    17281 tgtgtgacag tttatcacta cacaaaaaat cctacgcttt tttggttggc caatctcatg
    17341 aacaatcctc atacagtggc cacactcgat gttagcacag atgaaaagca aaaagtgatt
    17401 gatcaaatca atcgtgcctt agatgaatta aatgatcgat cagataatga acgaaaagcg
    17461 actgaccgaa ataaaagcgc agaagaatac ctggaactag cggaatatgc tcgtaaattg
    17521 acttctaatg gggaaatttc taagcaagtc aaagtgagag tacatgttta cggaaacacg
    17581 attcaagaag tagatgaacg agcgcaggaa ctgatcgaaa atctaaaagg gatggattat
    17641 cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt taccatgttc
    17701 gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc gttaaacatt
    17761 ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggagggac acaatatggg
    17821 gaaaccatga caggcggccc atttgtctgg aatgcgacgt ataaaaatag tgtacggttg
    17881 tctactaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg
    17941 atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac
    18001 tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg atctgacgga
    18061 atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcatc cagtttatat
    18121 gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacacactta
    18181 gacaaagtgg tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca
    18241 gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc acgcggcttt
    18301 acttcctcaa attcgcgttc aaatacggag acgcaagtca caggtaaaga tccagaagct
    18361 tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc
    18421 caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg
    18481 tatggcatga tttttgatgg gcatagtacg attcgacacc ttgaccaaca acagttggtc
    18541 ttttttgaca ttgactctat tacaggctta gatcgagggg tacaacaatg tcaaatgtac
    18601 atggctatga cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgacgttta
    18661 attaaagaaa aacaaatgga agaacaagac ttcaaacgct tcttggcgat gattgatgag
    18721 tgccataatg cgattaatcc agattttatt gaaacggtaa attatgtcac aaactttcaa
    18781 cgagaaatgc gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg
    18841 gtgccagaaa atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct
    18901 tctacgaaga tctttatgcg tatggatgaa tctattttac gtcatataga aggattagtg
    18961 ggtaaatcaa tgacgaactc agaattagaa tccattcctc aacaaaatca aggagacgct
    19021 gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa
    19081 ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaaacagt
    19141 atgttaagcg tcgtgcaaag cggcgcttat ttttgtgggt gtttggtact tctgcaggtt
    19201 tgattactat attgatcact gtgtttgtga ccctttttct aatacttgcg gcaggttcta
    19261 ttgataattc tgattcaagt agtggtggcg aagcttttac aggagaatat tcggaaggct
    19321 tacccattta taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg
    19381 ctgtaggtgc agcagtgaag tataaattat taccaagtgt gatcctttcg caatacggtt
    19441 atgaatcagc gtttggtacg tcggcgtccg caagaaatga tctcaattat tttgggatta
    19501 cgtggtttga tgggtgtctg tttcctaaag gaactgcgcg tggaattggt ggcattgaag
    19561 gtggctggta catgaagttt cccaactcaa aagctgcctt ctcctattac ggctttatgg
    19621 tagccacaca atcaaatttt aatgcttgtg tcggaaataa aagccctggc gcaagcttat
    19681 tgatcttagg acgaggaggg tatgccgccg caggaattac tgaagacagt gcctattata
    19741 aaaattgtat gtctattatt aatacaaata aattgatgga gtacgatgag tttgcgatta
    19801 aacactggag agaaggcggc ggaactggtg gtacgattac aggaagctgg ggcaatccat
    19861 tcccaggaag tagcttagat aaaaattcgt tcagtggagg acaacttttt gggaaaaatc
    19921 caggaggaga attccgacct aacgggttcc atgatggact agactttggt tcagtcgatc
    19981 atccaggaag tgagattcat gcggttcatg gcggaaaagt cgtgtacgtt ggcaatcctg
    20041 gtatttcagg tttgggtgcg tgtgtgatcg tgattaatta cgatggcttg aatatggtgt
    20101 atcaagaatt tgcaaatagt acaggaaatt ctcgagtgaa agtaggcgat caagtgaaag
    20161 ttggtcaagt aattgggata cgagatacag cacacttgca tttaggattt actcgaatgg
    20221 attggcgtca agcacaagga catgcattca ttgatgatgg tacatggatc gatccgttac
    20281 catttttgaa tagcagcaaa aaatagatca ggagaaaaaa tatggaaaat aaaaccttaa
    20341 aaatcgtctt aacagctgca attagcgtta gtttaatcgg caatattgcg ttgttttggc
    20401 agcttaaaga agaacaagag acaaatacct ataaaattga agaagtggct aaaaacgcgt
    20461 ctgataagct caaaaaaaat tcttctgagg cacaggcgaa gatcgacaag ttaaatcaaa
    20521 ccatcaatga gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa
    20581 aagaggaagt aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg
    20641 ataaatcatt aagcagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag
    20701 tgatcgacaa attaagtcct gcagacgatg atcaccaaca tgacgactac ggaagttact
    20761 cttttacatt aggtgacgtg caaacttacg ctcaaacaag cagtgtaaaa gaggaacaaa
    20821 attttgtcgt atttgttgat tatacaatcg gcaatcctca atttaaagat gtcaaaccac
    20881 aaaaaataaa aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca
    20941 cagactttag ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagcaaga
    21001 caacgtttta aaaaacgaaa aagttatctt agatggctgg ggatctttat gattctcggc
    21061 tttttgattc ttgctagtcc gatttacaca cataaaaagg tagacatacc agatgaagta
    21121 agcacgaacg catttttaaa cacgaaggaa ctgacgatgg tttcaaaaga aaaaggagcg
    21181 aaccagcaat tagtttttga atttgcgatc gacgatgaag atcagagctt attaaaagaa
    21241 ctagctaatc ttaattatcg tgtagaagta aagacagcta aaggggatta tcaagcaata
    21301 aaagccaaag tgatcaaggt gtctgatgat tattttgtag tgaagttagc acatgtacca
    21361 gaagaatata tcgccatgcg tttaaccatt attcctgaaa aaattgatcc aaaagtggat
    21421 atgcaagaac cacaagatct gatttattac gttcacgaag ataaggttaa agatcaagtg
    21481 aaagatgaag attatgaaca acatgcgatc aattacaaag tgaaaggata caaaaaagaa
    21541 caaaaagcag cacaaaaaca aatggaatcc ttccaagcaa ccattgattt gaatgaagaa
    21601 ttagtgaaga agctaaaaga tcaattgcct tatcaagtcg cagatgatca ggaaaatact
    21661 gaaaacaaga ttaatagcta ccagcaagaa attgaaacat cgaaaacgca aatgaaagat
    21721 caagaagaga tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaagatt
    21781 tagaaggaca ggtggagtga aaaaatgaag cgaacaaact tggctgacaa agatactttc
    21841 gctgagcaga aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt
    21901 cgagaagtga atttatctaa tacaacaata tctgagttga agaatggtcg aaagaattta
    21961 gatactttaa aattcagatc atttgaagct ctatatgtag ctgctaaaaa atattttaca
    22021 gctgataaca aataaaaaac aggagtattt tatgtgaaaa aagtatataa aaaatgctta
    22081 tgaatcagct atatcacctt aaccatttgt taatgagatg ggagaaaaca ttaaccacga
    22141 ttctaacgca aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt
    22201 tcaaccaaac aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt
    22261 tcatatgaca aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa
    22321 tacacaaata accttaattt ataaagagca taaggaaacg tgggaacact ttttaaaaaa
    22381 agtgttccaa taggctgtgc ctattacgtt tctcaaaaat aattgtggga acaaaatatt
    22441 taaaaatagg atagtgaaag gatagcaaag gatagtaaaa ggatagaagc aggatagcga
    22501 aaaggatagg ctccttttcg ctatcctttt taaattggag gaattaatat ggaagtaaga
    22561 ataagaaatg tagatgagaa aatcgtaaga gaaattgatc gaagatgtga cgaactaagt
    22621 aaccgaacgg gtaataaatg gagtcgtaac gattatttaa agctactcgt tgaaaatgat
    22681 tttgatcgtc ctttgatgga atataaacaa gataaatttg atcaattatt aaatcagtta
    22741 atagataaat tttcttttgt tcaaagtcat aatacaaaag tattagaaga atatatttgt
    22801 cagaataatc aaattatcga acttttaatt gaacaaagta gaggaggaga agtttaacat
    22861 ggccaaagtt atagacaagc atatttattt ttatgaaaca catatagatg ttctagatga
    22921 aattattgca actcatgcag aggttaaaaa ctatagtgaa gccgttcggt ttctatgtat
    22981 gaattatgat tcagaagtgg agaaaaaagt aaattccaat caagcaaagt tgaatgcaat
    23041 gggaaaagaa atttctattt taaccgaaat ggtttcagag tttgcggatc tccatttgaa
    23101 agatacaggg attttaattg gtacaaattc aactgtttac caagaagcaa aagagcgtgt
    23161 ggaaggcaag ataaaagcaa atcaaacgaa gaaatattct cctaagataa agaaaacaat
    23221 aagcgaatca gtgactacaa aaagtgaagt atttgatcca acaaggttct taaaacgata
    23281 aaaattagaa ggagaatgtg atgagtaaat cacctgcagt cattctcact tgtcaattta
    23341 ccttgcccaa taaaaaatct ttttcaacat acattgatta tatgacaaga gaaaaagcgc
    23401 tggaagaaaa ggaacgacgt acgcccgagg aagaacagga actgcgtcta atcaagaatg
    23461 ctttagagga tttttatatt cctgaaggag aaactttttc ggagagtaag acgggtaaag
    23521 aattcagcga aacgtcgaat gaagcgcaat cgttgatgaa acatggaatc aattttgaca
    23581 agttagaaga acaagatttt accaaatatc tttcttatat gacacgtcac tatgcgttag
    23641 aacaaaagaa agtactgacg catagtgaac aaaaagaata ccaacgcttg aaccaagcaa
    23701 taaaaaaata tcaaacagag ccacacaaaa aagcttcagg aaatcttcct ggagtttttt
    23761 ctatggcttc agacgaagtg aaaggaagtg atctgaagga gatcaagcaa accttccaaa
    23821 gaggacaaca aaaaggctca atcatttatc aagatgttgt ctctcatgat cattcgtatc
    23881 ttgaaaaaat aggattgtac gatcccaaaa cagatacttt agatgaagaa ggtttaaaag
    23941 cagctggaag aaaaatgatg gagacattgt tcgagaaaga gcaactgaat gaaacaggct
    24001 actggatggc aacgattcac cgaaatacca aacatatcca tattcatttt gcagttgttg
    24061 aaaaaaaaaa tacgcgtaaa ccatacactg agattgaaaa tggtgtgagt tatatcgtac
    24121 cgaaagggaa gagaaggcaa gcaacattag acgaaatgaa gactaattat gtacatgagt
    24181 tagaacgttt ttcttatgaa aaaaatcgaa tgggtatgga gaaacgaaat ttattgacac
    24241 ggaaaagtga tttgcgtaac accttaacca aagtagtgaa agagccagta cgttatgatc
    24301 gacacgcaat gagcttatta actgaagtat ataaaaactt acctaaaaag cgttcggaat
    24361 ggcattacgg ggatgaaaat cgaaccaaac tttcaccata cacaagagag aaactcgatg
    24421 agttaaccca atatgtgtta acaaaagaac ctggatatca agaatatata cagatttcaa
    24481 aagcattgaa agaagaagca aaaagtcttt atggggaaag caagagagaa tcaaaggatg
    24541 cagaaaaaaa tgctctcttt gatttaaaaa agcgcacagg aaatgccatt ctaacagaac
    24601 tgaagaaaca ggatagtcaa ataaaacctt taatagaaag gatggatcag atgcaatcta
    24661 tttacaatga acgatccttt cgaacactta aacagttgcc aacgaatgaa tacaaatata
    24721 gttacgaaga attcatgaag cgcaggaaac aattccaaca aagagttgtc acgcaacgga
    24781 gtttgcgtca attaaatcga acgattaatg cccatgaaga aaaataccac gcaagtaaag
    24841 catacgaaga gcaacaacga agaattcaaa tggaacaaga aagacagtag gaggaatagc
    24901 aatgaaacaa tatttagatc aatggaaagt tatagaagga tcattaagag aagaacgaat
    24961 cgaacaactt cccgattgtt tagaaaaaga gcatttgttt cagataagag aaatgttgat
    25021 aaacgaacag tttgatccaa atcaattttt agtcgttgag tacccagcta ctggtgtgta
    25081 ctgttgtaat catgtgaagg gtgaaaaata ttttatcatt caagaatatg agggcaagtt
    25141 agcgccttat tatactactt gggaaatgaa tgaagagggg atcaataact tcccgtgtaa
    25201 aagcattgaa gagtctattt cactcacaga atgttagttg ttaagtgaat aaaagaagta
    25261 aaagagtcct gaacgtgatt gtccaggact cttttttttc actgaattgt tttccgccaa
    25321 ttcattgagt ggtgcgattt ccgtctacat tatatcaaaa gaaaggagga agaagaaatg
    25381 gcttattcaa cgattggagt tgttgttgaa aaaagcagag acaatcttgt ttttgtgaca
    25441 gaaattcaaa caggcagagc ttttgtagtc acagacaaag cggcaaaagc atatcaaagt
    25501 ggcgatattt taacgttaaa tctgactacg aagacgttcg tcgatgcggc agaagattat
    25561 ccatt
//
LOCUS       DAYYAO010000037        24970 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000037 DAYYAO010000000
VERSION     DAYYAO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.039"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..519
                     /locus_tag="ACHARI_001423"
     CDS             <1..519
                     /locus_tag="ACHARI_001423"
                     /inference="COORDINATES: protein motif:HMM:NF028094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8442475.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            512..1042
                     /locus_tag="ACHARI_001424"
     CDS             512..1042
                     /locus_tag="ACHARI_001424"
                     /inference="COORDINATES: protein motif:HMM:NF019106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFM8442476.1"
                     /translation="MNELWMDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIM
                     KPSDNGKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVEN
                     LEWLSSDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRH
                     TVLNIASGKTFSFLED"
     gene            1147..1977
                     /locus_tag="ACHARI_001425"
     CDS             1147..1977
                     /locus_tag="ACHARI_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8442477.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1994..2845
                     /locus_tag="ACHARI_001426"
     CDS             1994..2845
                     /locus_tag="ACHARI_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8442478.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2842..3201
                     /locus_tag="ACHARI_001427"
     CDS             2842..3201
                     /locus_tag="ACHARI_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8442479.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            3216..3377
                     /locus_tag="ACHARI_001428"
     CDS             3216..3377
                     /locus_tag="ACHARI_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8442480.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            3374..3679
                     /locus_tag="ACHARI_001429"
     CDS             3374..3679
                     /locus_tag="ACHARI_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442481.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            3679..3996
                     /locus_tag="ACHARI_001430"
     CDS             3679..3996
                     /locus_tag="ACHARI_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8442482.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            3993..4388
                     /locus_tag="ACHARI_001431"
     CDS             3993..4388
                     /locus_tag="ACHARI_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8442483.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4385..4723
                     /locus_tag="ACHARI_001432"
     CDS             4385..4723
                     /locus_tag="ACHARI_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442484.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4720..5088
                     /locus_tag="ACHARI_001433"
     CDS             4720..5088
                     /locus_tag="ACHARI_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442485.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            5085..5390
                     /locus_tag="ACHARI_001434"
     CDS             5085..5390
                     /locus_tag="ACHARI_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442486.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            5414..5617
                     /locus_tag="ACHARI_001435"
     CDS             5414..5617
                     /locus_tag="ACHARI_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8442487.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            5614..5910
                     /locus_tag="ACHARI_001436"
     CDS             5614..5910
                     /locus_tag="ACHARI_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442488.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            5986..6402
                     /locus_tag="ACHARI_001437"
     CDS             5986..6402
                     /locus_tag="ACHARI_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8442489.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            7310..7654
                     /locus_tag="ACHARI_001438"
     CDS             7310..7654
                     /locus_tag="ACHARI_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8442490.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            7659..7940
                     /locus_tag="ACHARI_001439"
     CDS             7659..7940
                     /locus_tag="ACHARI_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442491.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            8043..8357
                     /locus_tag="ACHARI_001440"
     CDS             8043..8357
                     /locus_tag="ACHARI_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8442492.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            8335..10029
                     /locus_tag="ACHARI_001441"
     CDS             8335..10029
                     /locus_tag="ACHARI_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8442493.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            10049..11227
                     /locus_tag="ACHARI_001442"
     CDS             10049..11227
                     /locus_tag="ACHARI_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8442494.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            11190..11876
                     /locus_tag="ACHARI_001443"
     CDS             11190..11876
                     /locus_tag="ACHARI_001443"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8442495.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            11876..13036
                     /locus_tag="ACHARI_001444"
     CDS             11876..13036
                     /locus_tag="ACHARI_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8442496.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            13046..13921
                     /locus_tag="ACHARI_001445"
     CDS             13046..13921
                     /locus_tag="ACHARI_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442497.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            13918..14229
                     /locus_tag="ACHARI_001446"
     CDS             13918..14229
                     /locus_tag="ACHARI_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442498.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            14219..14572
                     /locus_tag="ACHARI_001447"
     CDS             14219..14572
                     /locus_tag="ACHARI_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8442499.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            14562..14963
                     /locus_tag="ACHARI_001448"
     CDS             14562..14963
                     /locus_tag="ACHARI_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442500.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            14956..15360
                     /locus_tag="ACHARI_001449"
     CDS             14956..15360
                     /locus_tag="ACHARI_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442501.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            15372..15980
                     /locus_tag="ACHARI_001450"
     CDS             15372..15980
                     /locus_tag="ACHARI_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8442502.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            16000..16362
                     /locus_tag="ACHARI_001451"
     CDS             16000..16362
                     /locus_tag="ACHARI_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442503.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            16365..16547
                     /locus_tag="ACHARI_001452"
     CDS             16365..16547
                     /locus_tag="ACHARI_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442504.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            16565..19897
                     /locus_tag="ACHARI_001453"
     CDS             16565..19897
                     /locus_tag="ACHARI_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8442505.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            19948..20685
                     /locus_tag="ACHARI_001454"
     CDS             19948..20685
                     /locus_tag="ACHARI_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8442506.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            20695..22986
                     /locus_tag="ACHARI_001455"
     CDS             20695..22986
                     /locus_tag="ACHARI_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8442507.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            23010..>24970
                     /locus_tag="ACHARI_001456"
     CDS             23010..>24970
                     /locus_tag="ACHARI_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442508.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGS"
BASE COUNT         8961 a         3726 c         5379 g         6904 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac ttccgtttcg aaggtgtcac agggcgcaaa
      121 ataagcctca aagatgaatc gagcacaacc gtaagtgatg ccaatatctt agcgaatatc
      181 atcctagatt tcatctttga gcatgatatt ccgtttaaaa atggatatga aattttgccc
      241 caaaacgaac agtatttctt ttacaaatgc ctcacgaaca gggtgtgctg tagttgcgga
      301 aagaaaaatg ctgatataca tcacgtagac tctgtcggca tgggaaataa ccgaaaaaaa
      361 ataaacaatt ctggtaggcg ctttatggct ctgtgcagag aatgccatac gaaaattcat
      421 gtcgaaggtt ttactacatt tacaactaaa agaaagctta cggcagttgt tctcaaagac
      481 agtgatttaa aaagattggg gttgaatatc attgaatgaa ttatggatgg atatcgaagg
      541 atacgaggga gtatatcaag ttagtagctt aggaagagtt aaatctaatc gccctaacta
      601 tgtaattaat aagggatacg ttaacaaaga aggatttatt atgaaaccat ctgacaatgg
      661 gaaagggtat caaattatct tcttaagcgg caaaggtttt agagatagaa ggtatgtcca
      721 tcggctagta gcttttcact ttttaaaaga agcgtatttc gaaggtgctg aagtcaatca
      781 taagaacgga gataaatcta acaatactgt tgaaaaccta gaatggctca gttctgatga
      841 taataaaaag catgctagaa aaaatggatt aactaacttg aaaggaccat caaaactaac
      901 tgatttgcaa gctttagcta tcaagcgttt atatacaaac aaattgatga gctccggaga
      961 aataatgaaa ctttttaatg tcagcaggca taccgtgctg aatatagctt cagggaagac
     1021 atttagtttc ttggaagatt aagagaaaca caaaataggt gtgactaatt tcaaaaataa
     1081 atatcaaatc aaagggatca agttaaacca ggaaacaatt aaaaagttaa ggataggagg
     1141 ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa
     1201 atacgattaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg gattcgatta
     1261 ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag gaacatgcct
     1321 tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt tcgcttagcg
     1381 ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact aattgccatc
     1441 agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct aaaaaatgct
     1501 gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac
     1561 gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa agatatagat
     1621 aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa aaagttgcaa
     1681 agtaatttaa ctgaagattt tccaaaagaa atgaacaaag tagatatcga aaaatgggca
     1741 gacacaatca ggttgatgga agaaagagat aaagcatcta tagaagcgat tgagtatgtg
     1801 atcaattggc tacctacaaa tgaattttgg tttggaaata ttagaagtgc taagaaattg
     1861 agagaaaaat ttgagaagct caaattcgaa atcaaagcag acaagaagaa tcataaaaag
     1921 caaagtcaaa aactacagta cagcaatcct agtgaatatg acgacttgcc aatttaaaaa
     1981 ggagatgcat cacatggaaa gcctagcaaa tgctatggag aaactaataa gaagagtatt
     2041 agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg tatagttggc gagctaaaaa
     2101 taaggatggt tcagaacgtt gtaaaccaac atgcatgagt tgtggttata aagcgttacg
     2161 tgtgaaagag gatatacaga ccgaacggat atataacgat agcttaaaag cacgagcgtt
     2221 gagttttttt caaaatggtt cggtattaac agataaaact ttgtttaaat gcaaaatgga
     2281 aaattatcac gtagtggatc aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac
     2341 gaatgaggtt ctgctgaacc atcctgcaca tttcattcta tcagggaaat caggaagcgg
     2401 aaaaagccac ttatcaatgg ccacagcttg ggaaatactt gagcgctcaa attatgacaa
     2461 gaaaatactt tttataagct atcaagaatt attagagcaa ataaagtttt cttataacaa
     2521 tactgaactg agaaaagaaa ttgaaggatc gcttatagcc gatattaaaa caactgattt
     2581 ggtggttttt gacgatattg gagctgaatt aggtagtggg gtatcaaata gtaggcagtt
     2641 tacaaacaac acgttaaaca cgctcttaga agccagacag aacaaggcaa cgatcatcac
     2701 aacaaactta tctggtcctg aactaagaga agcctacggt gaaagaattg tttctaggat
     2761 atttaagaat tcagaaggtt atgcgctgaa attccaacaa acagcagaca agcgcataaa
     2821 accagtgaaa ggtagtatcg catgaataaa taccgtaata aaaaaactgt tcatcgaggt
     2881 atcaagtttg attctatcgc agaagcagag tattacgatc tagccttgtg gcaagctgaa
     2941 gcaaatggct ggaaagtaaa acttcaggaa agatttgagc tgatgccgaa atttgaacta
     3001 gacggaaaga agtatcgcaa gatcgagtat attcccgact tcacatttta taaaaacggc
     3061 aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa aagactttaa gatcaaggca
     3121 aagttgttct gtcatcaata tcaagcgccg ttgatattag ctaaaaaata tcggaatacg
     3181 ttcaaggaag agcgttttta acgaggtggt ccatcatgac aacagaagaa gtgattcaaa
     3241 tgcgtattcg aaacattcag cgtgaaattg acgatctaga acgaacaaag gcagtgatgg
     3301 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaacttaaga agggaaattc
     3361 gtagattgga ggaatgagcg tggataagaa agcgacaatg aaacgaattg ctgaattaac
     3421 caagtcagaa tcttggcagg aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     3481 atcaatgtgg actgaaaagc ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg
     3541 tgatcgaatt ctagtaacag gtacagctga acagttatct gaaattactg gattaagcaa
     3601 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
     3661 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
     3721 atcatgatga agattcagaa agacaaaaaa tcaaaagaac gagcggaatt agttaagttg
     3781 gaaatggctg aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac
     3841 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
     3901 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca agtttgtaaa gaaatataaa
     3961 gagatattag acaaatacat ggaggacagc aaatgatact gaagtacagg gcatggaata
     4021 agaaaactca gtcatttatt gattatggtg atttggtttt agatttgaga agcggaaaaa
     4081 tttacgctgg ggacattggg ttagtggaaa gcaccattga tgtgactgac caaattgaac
     4141 tcatgcaatc cacaggaatg aaagataaga atggtgtgga gatatttgaa ggggatgtag
     4201 taattacatc aaaaggagcg attggatatg ttgcgtatct tcaacaagaa gccggctttg
     4261 tagttgtact aaagaaaagt gattatagat taggccatag gaatactggc gaaagttatg
     4321 atgtagcaac tgctcatgaa gtcatcggaa atatatacga gaatagcgag ttattggagg
     4381 tagagtgatt atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt
     4441 cccagcattg ctaaattgtg gaattaaaag caagaataga atcattttgt tacggatatc
     4501 ggctgattta gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac
     4561 acgttatcag tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc
     4621 tgacatgcat ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc
     4681 cgatgatttt ccaaatttat ggttgaagga ggaagcggaa tgaacgagga aataaaaaaa
     4741 tctattcgtt acattgatag agcgttatcg caactcgaac ccttgcgaat tgaaagtcat
     4801 aaaggaatga gtgtctaccc aaatcagtat gacaaaatga acgctgattt acgtaatgca
     4861 aagctggcct taattaacat taaaaataac aaaggctgcc agtattgcaa acacagcgaa
     4921 ccgttgaatg ataccagcaa ctctaacttt gcaactgcaa ttgagtgtga ggaaaatgga
     4981 tactacattg caactgagta tgacgatggc gattatgcag ataatggcta ttctgcttat
     5041 atccaatatt gccctatgtg cggtcgaaaa ttggaggtag cggaatgaat gttcaagttt
     5101 atttaaaaag aaatttggcc aggtttcctc gaagctggga tacaaattcc cacccatccg
     5161 aggcagaaga agagagctat ctaatgtctg ctaacatgat agaaattaca agagatgatg
     5221 cggatgagtt tgataaaaaa agatcatggt actcggtttc gaatccaatg tatacggctg
     5281 taatagaaga ttatcatata tccgacagat ttattctggt tgatctagaa agacctaaga
     5341 aacgaattaa acagtataga cgttgcgggt tttcaacaaa gaaatggtga aaacaaaaca
     5401 gaggtgactc agaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact
     5461 gaatgtacta aagacaaagt ttgaaaaaga atacggagtt tctgcagaag aaacatataa
     5521 agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg gaatttcgat
     5581 attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg attttacgct
     5641 agtagccacg gtatcggcgg tttaatgctg gatatgcaga caaagaaccc taaaacacgt
     5701 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg gttcatctat
     5761 ccatttcgtg gatatgtaga acatgtctat aatcactcag cgatcattcg cattgaaaac
     5821 acgatggaat gcgacaagtt attagcgaaa agcaaagaga atttagcagt ggtgagattg
     5881 gtggatattg aactaatcaa tgacaaataa aaaagccgga tcgctccgac taacataata
     5941 aaacagacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
     6001 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     6061 ttggagcgta tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
     6121 ccgaaggcgc cgaagcacgg caataaggca gaggacgcga tcattcagat gatggatata
     6181 gaagcagaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
     6241 atactctact acagcttctg cgacgtaaat aagcattcta attatgaaat aggtcaattg
     6301 atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt gattgaattt
     6361 gctgaagcat ataaaaaagg cgtattaatt cagtatcgtt gattttgtag ggtttttgta
     6421 gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag
     6481 gaaacaggac ttcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
     6541 gcttcgatag acagcagcgg aaatattaag aataaggatg tgaattttaa ctccttctaa
     6601 attgttctta ttatctatca tccgttgctg tctattgtca ttatgtcact gtggcggaaa
     6661 gggtagacgc ttaaaaataa ggtcaatacg tcgagggata gccttaacgt tttatgattt
     6721 gaccatgcaa tgttcgattc attgccagtg acttaaggaa cctacggaaa caattcatct
     6781 tatcggatgc cgatgaattg gctgactagt cgggatgcta ctagcagtta gaaggcataa
     6841 aatactagcg cagacgtgcg ccactctcag gtgtaggtta ggagagaaac attagttggg
     6901 gttattagga atacgataac ctgcttgcga caaagctttg tactgtcgcg ttggtcatga
     6961 acagagacgg tattctgttt cagtattcgt tagcaaccga gggatgtggc agtggtgagg
     7021 tgcaggaagt atcagacttg tttgtgtgca agttactatt acatattaga tcactctttg
     7081 agtggtcttt ttattttgcg taaaggaggc acgacgatgt atagaccaca atacttagaa
     7141 cagaagcatg aagtaatcac tgtgcaaaac ggtaacggtg agatagtacg aaagtataga
     7201 agaccaataa agagcgatac atataaacgg aaggaaagca atgaagttat tccattgtat
     7261 ggcaaaagaa tagctaagta ttaaataaga ttgcgaaagg agatgggaca tgaccgagga
     7321 attctataga tggttattac agttgataag agaagatcgt ttggttaagt tctatcagtc
     7381 tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg aatgccaaga
     7441 gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa aggaagtcaa
     7501 ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg ttgaacatca
     7561 taatgaagtt catggcagat atcttacagc gttagataaa caagagaaga agatagaaag
     7621 cttcgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat gacaatggca
     7681 gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga ttaattaaac
     7741 gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac tgttgtagta
     7801 aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc gaaagaataa
     7861 aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc gctgaagctt
     7921 tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt gggggtgatg
     7981 aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa aggagggcta
     8041 aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc cgctctttaa
     8101 taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat gttgatcgtt
     8161 atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag aacggcatta
     8221 gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa
     8281 tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat
     8341 caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga aggcactatc
     8401 aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt ctttactaga
     8461 gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc agcacaatac
     8521 tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta tggtgttcga
     8581 tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg acgtggtaat
     8641 ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa acacggtatt
     8701 aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac tagttttgat
     8761 gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt taaagctacg
     8821 aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac ggcaaatgct
     8881 agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca cgaaaatgaa
     8941 gattattcaa tgataaatgt ggctacttct ggtggtggta aaaatcgaga ttatagagaa
     9001 ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa
     9061 tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga attttctagt
     9121 ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc cgacatgtgg
     9181 gaaaaagctt gtccaactat taattacaat gcaaatttaa aacggaaaat gtttcaagaa
     9241 tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa acgaatgaac
     9301 agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct gcatacgaaa
     9361 gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt tgctgataga
     9421 cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta ttttacacaa
     9481 catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga ggttatagat
     9541 atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga agctgattat
     9601 attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat cgctatggat
     9661 atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac tgtggaaatt
     9721 gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga cctttttatt
     9781 aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat gaatgtatat
     9841 gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga aactagaaaa
     9901 acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat ttatgattct
     9961 gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt tactcctcta
    10021 gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg taggatactt
    10081 cacaaaaaaa gcaacggtta ctttagaaga atacttttgt aaattgcaag ttgattttgt
    10141 gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc
    10201 ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact ataggctgaa
    10261 tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga tcaggagatt
    10321 aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg aaatatttat
    10381 tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt ctcaagtgca
    10441 aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct atataaaata
    10501 cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg ggaaactcat
    10561 ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac tgaaagggaa
    10621 tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga tgacatcaca
    10681 atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac aaaatgagta
    10741 cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa aaaacttaat
    10801 agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg
    10861 agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa tcataccgat
    10921 tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag aatacttgtc
    10981 agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca aagatttggt
    11041 tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag gaagagaacg
    11101 cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa ctaaaaacaa
    11161 tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa
    11221 agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct agttggttct
    11281 ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt gatcaagaag
    11341 aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt gctattggga
    11401 acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta gccgctagtg
    11461 ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat gcacaattga
    11521 tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt acgattgctg
    11581 accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga ttcaatggca
    11641 cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca gaaacagctt
    11701 tgagttatgg attggtcgat gaaattatcg atgcagaaaa tagctcaggt actgaagcta
    11761 aaaaagaaca aagcgttgaa gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa
    11821 ttgctgcatt tacagcagca ttaaataaaa catttggaca aggagatgta aaataatgac
    11881 agttaaaaat ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag cttttaaaga
    11941 tggtaacgaa gaatctttta gcgcagctat ggtaagctta tctaaggaaa ttcaggataa
    12001 aattttagaa gaagcaacag caaaaaatca agatcaatta gtattaatga accgtggtca
    12061 gcgtgtatta actacacaag aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt
    12121 tgcaggggtc gaagaattag tgccagctac tgtatttgaa cgtgtatttg aagatttaga
    12181 acaatctcat ccactattgc aaaaaattac ttttgttaac acaactggtg taacagaatg
    12241 gattgtgtca cgtggagtca atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa
    12301 agttttagat aatggctttg atgtaattaa catgaagcag ttcaagctat caggttatat
    12361 tcctgtatgt aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt atgtccgtac
    12421 tgttttagta gaatcattga gaattgcatt agaacaagca attgttgatg gtactggtaa
    12481 agatatgcca gtcggaatga tgcgtgacat gagcaaacaa actagcggag aatatgctga
    12541 aaaaaaagca gaacctatta cagctttaga tgctgtaact atgggcggtt tgatggcgcg
    12601 actatcaaaa ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc
    12661 tgatgtggtc ctaattgtga atccaactga ttactggtct aaagtattcc cagctaagac
    12721 tgtactaact gctaatggag aatacgtaca agtattgcca gtaccagttt cagatttgca
    12781 gtcaacggct gtgccagaag gaaaagcagt tattggggta gcctcagatt acttcatggg
    12841 tgtaggatct acactaaaaa ttgaagcttc agatgaatac cattttgttg aagacgaacg
    12901 catttatcta gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt
    12961 attagatatt agcgctttgg gaactactac tacaactaca aaaccaacaa ccacaacaac
    13021 tacaacacaa gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg caatcaatcg
    13081 gtttaaatgg gagcttgaag tttgtttaac taatctgaag aaactaggaa tcaaagatat
    13141 cgtattgctt ttcagcagac acgatgatca gattcctatt ttttttgaga aggaatatgg
    13201 tgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc cttcgattaa
    13261 accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg accgatattt
    13321 ctatatcgat tcgaatgtca ttttcaataa aagaattaat ttgcgcaaat tgccttctaa
    13381 agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt atattagaag
    13441 ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaac attgtaaatg ttacagtaga
    13501 atctttggaa actataaaca ctaattcagg aggcgcacag tgggttatta accgtcctaa
    13561 agcgaattat tggaaaaagg tttatctgga ttctaatcgg ctatatcgct accttagagg
    13621 gcaaaaaaca aatatacaaa tctggacagc cgagatgtgg gcacagcttt ggaacatgat
    13681 gtatttcaat attggtccta aagttcacga ggaattagac ttttgttttg ctactgatcc
    13741 aatagaaaaa gttaaagaag taaaaatctt acacaatgct ggagtaacaa caaacgatga
    13801 agatttattt ttcaaaggga gatacgtgac ttctacgcct tttgatgaag atttatcatt
    13861 tgtaaacaag aaaaaatgct cttacgcata tgttaaagca attaaggcgg tggttagatg
    13921 acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat ttacgttacc
    13981 tggaacgaag aagatgagtc aattaaaaag atgatagcta aaaatgctgt ttatcttcaa
    14041 agtaaagtga gtacaacact ttctttttct cctgaaagct tagaatacgg attgctaatc
    14101 gaaagatgta gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt
    14161 gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg tgatgtgaat
    14221 ggcgaataat cgtagactcg aagaaacatt caacgatggt tggttaaaga ttttgacgca
    14281 aactaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag aaatcacttc
    14341 tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg atgcgatggg
    14401 atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga aatttaataa
    14461 agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg ccgattacga
    14521 taatttttat atctattttt atcttgaaag tgtgggtgaa tatggtgatt gataattcta
    14581 aagaaaaaga acgtttaaat aagcaaattt ctgctatcaa aacttcctta gaagagcatt
    14641 ttggcctcaa actctttcaa gactccgtag gcgaggatga gctacctgat gattttaatt
    14701 acttcattct cgaaacagga gaaatagaaa tgatcactga gccaaaatat agcgtgggtc
    14761 aaaatctata tctaactttc tattcagaaa atagagaaga tttaacagga gactcactag
    14821 atattatttc attgattcaa aatcgttcga ttcgttttca gagaatggat cccaatcatt
    14881 taaaactaga gaaccaagat cgctatatcg atcaattggt atttacgttt agacgattat
    14941 tgaagagtga ttgtcatggc taaaaatagt tgggagctaa aaataaatgg acatgatgaa
    15001 cttcttgtgc ggatggaacg ctattcaagc gagagcgaac gactgattaa cgaagcattg
    15061 aaatcaaaag gttcggctat tgcagtggat aggattacag aaaaaattcc tgtttctgaa
    15121 gcagatttaa gaagagggca ccaacacgca aaaaatagtc gtccacttaa gactcaatac
    15181 attaatttgg gtttcatcat tagacctaca agaaaatttg agtatttaaa atatcctgat
    15241 ttggggatag gtacttctaa aagaaatcag ccggacgaat ttatgagaag aggattaggt
    15301 cttgcacttg atccaattac agaacttctg attcgtcaat tcgataaatt aaataaatag
    15361 ggggaacaac aatggctaaa acaaaaactg tagtaacaac gttcgataac ataagtatca
    15421 aacgaattgc ttttaatttt aagaacgcag gaaatgcaat cgtaacgaat tgtaatggac
    15481 aattagatgg cgaaacagaa atgcaaacgg tggttaaaaa atgtggagcg acagaagtaa
    15541 aatcaaaatc taaaccaatc aatatgacgg taacaattac tgcacatgta ccgatggaag
    15601 tttatcgacg tttcaatggg ttgaaacaag atggacgtat taaaccaggc atttactctt
    15661 acggtcctga ttccgtaggc gaagatttct cacttgctgc agagatcgtg gatgacttcg
    15721 aagaaaatag aaagttagtt ggtatgttag catgcacttc gaatacagga ttaacattct
    15781 ctattgaaaa tggtgcggat gaagtagctg cgttagaact agaaacaaaa gttatgcaag
    15841 atgaatttgg taaattttat catgaagcaa ttgttgcaga acttgaagaa gacttaacag
    15901 atcaatggat gacgaatcta tctgctgatg tgattaaaaa aacttcagtt gtgacaacta
    15961 cggccactca atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa
    16021 attgaactaa acgatggaac aattttgaat ttagaaccta aactgaatat caagaaatta
    16081 ttgatgatca atagagattt taacacagac gagtttgcaa aaatgtcgat gggaaaaggc
    16141 tctatggata ttacagttat tcaaggtgca aaagccgtat atgtagctta tcgtcaagcg
    16201 aacatggtcg attacatttc attcgatgaa tttatagata aatgggattt tgatatggag
    16261 gttgcagtag ctgtatacag tactatgatg ttcaaacaag cacgtgatgc ctatcaaaaa
    16321 gaattcgaaa aagcaaataa ggaaaaaaag cttcaaaagt aaaaatgcca aagctcttag
    16381 ttgaaacgtg ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg ccttcagatt
    16441 tggttttaag cgatatctgt ttagatgaca ttttgcaaat ggcttacaac aagagcgctt
    16501 atgaaggatg gaagaactat gcaataaatc aatcccagaa aaactaaagg aaaggaggta
    16561 aaaaatggct aaaaagagaa cggaagcaga agtaactttc atagctaacg atgacggatt
    16621 gaaatctacg ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact
    16681 agaacaagct caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc
    16741 ttctttagaa aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gtcaacgttt
    16801 agccaatgcc aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgaaagaga
    16861 actgattaat caacaaacag ctcagcaaag gttatctaat gatatagata aaacgagtaa
    16921 ggcattggct caatctaaag gtgaatttaa aacgtatgag tcaactatga aagacttaga
    16981 taatgagcaa caacatctaa agtcaagcgc atcattagta gaatccgaat ataaaaaatg
    17041 gcaagcaact gctggccagt ctgctacaga atctgaaaaa ttagcaaaag cacaagaata
    17101 tgtcggcaaa cagagtgata ttgctgaaca aaaaatcgaa gtgctaaaaa aacaattaga
    17161 tgccacacaa aaagaatttg gagaaacatc taccgaagct ttagaaatga aaactaagct
    17221 taatgatgcg gaaagagaat tcgaagaatt aagtaatgct gctaaaaacg ttgatacctc
    17281 cacagtagat gatatcggca aaaaacttga tatgggtaat ttaatggaag ctactgatca
    17341 cttgtcagtg atcggagata agcttattga tgtaggtagt aagtctattg aagcagctgg
    17401 aaaagcacaa gctatgcagg cccaatttaa acaagtcttt ggctctttag aaggggaagc
    17461 acaggacgcc gttgagggaa tggctgaaga atttggaatg ttaccaaata cgatcaagcc
    17521 tgtttttata caatatacgt caatgtttaa aggacttgga tatgatacca aagaagctat
    17581 ggagttagct ggtgatagta ctcagttagc agcagatgca gcagcttttt acgataagtc
    17641 tatggatgat gctagtgaat ctcttaattc atttataaaa gggaactacg aaggtgggga
    17701 gcaaataggt ttatttgcta atgatactca aatggcagct tatgctgtta agcataattt
    17761 gataccagcg actgaaggag caaaaaaagc cagtgaagag tcattgttag ctgttgaaaa
    17821 agcacaatct aagtatgctg atgctattaa gaaacatggt gaaggatctt tagaagcaag
    17881 agaggctgct ttaaaactta aagatgcgca agataaaata aatgaagaat taggcccaca
    17941 aacgcaaaaa tggtctgatt tagatgaggc taccaagcaa gcagttcgag ttcaatatgc
    18001 tgaagatatg caaaaattag ccggtgctac aggtcaggct agtagggagt ctgatggttt
    18061 agaaaaccaa atgacaagag caaagcaagc gttagaagat ttttatgcct cattgggtga
    18121 agatatactg cctgtgttta tcaaagggtt gcaagcagga gcgaaagctt tgcaaggatt
    18181 agccgaatgg tggagtaaac ttgatgggcc aatgaaaaat ttcattttag ctcttggagg
    18241 aattctagca ttattaagca cattagctcc tgttataacc gcagttgtta cgatagttgg
    18301 cacatttggt tctacagttt tgctgccaat aataggaatt atagcgggcg ttgcagctgt
    18361 aatagcgatt gtaattacag cgtttcaaaa ctggggcgca atcactgact ggtttagtga
    18421 tttatggaaa aaatttaccg attggttagg tgatacctgg gaaagtataa aagaaagtgc
    18481 ctcatcagtt tgggatggag tcaaagaaac ctggtctgga tttgtagatt gggttcaaga
    18541 aatttggcaa ggagtttctg attggtttgg agagttatgg agcggattag ttgaaggagc
    18601 ttccaacatc tggcaaggag tccaagagac ttggcaagca ttcgttgatt gggtttcaaa
    18661 tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt
    18721 tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg
    18781 tatctttgat gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat
    18841 ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca tcgcttggaa
    18901 tggaattgta actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat
    18961 atggacatct attagtacta cggcttcaaa tatttggaat tcaattagtg caacagcttc
    19021 tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt
    19081 aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag
    19141 ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg ctataaacgc
    19201 agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg
    19261 gaacggtatc aaaaatactg ctttgaatct ttggaatgga attacaagca ctattagctc
    19321 taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt
    19381 ttccgacata ttcaataatg ttaaaagtac aattgctaac atatgggaag gcatcaaaaa
    19441 gactgttagc gctccaattg attggattag agacaaaatc agtggcattt ttgataattt
    19501 gaatatttct ataccacata ttccgttacc ggaatttatc atggaaggca gctttaaccc
    19561 gctaaaaggt caaatccccc atttgcgtgt aaaatggcat gctaaaggag gtatctttac
    19621 taaaccaact ttactaggtg gaatgaacgg tgtcggtgaa gcaggacctg aagcagtttt
    19681 acctttgaaa agatctgttt tgcaagaaat tggtgatcgt atcttgagta gcacatcagt
    19741 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    19801 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    19861 aaaaattcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    19921 ttttagtatt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    19981 tttcctgatt atttgcataa attggcttta gctgaaagac caaccatacc ttctccaaaa
    20041 agacagagag ttgaaacttc tgttttaggt aggttaggtg gcttagtaca agattactcg
    20101 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    20161 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    20221 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    20281 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    20341 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    20401 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    20461 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    20521 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    20581 aacaaaatga agaccatgca atatccaacg ttgcaagtgg atattaatca ttttaattgt
    20641 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    20701 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    20761 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    20821 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    20881 gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    20941 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    21001 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    21061 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    21121 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    21181 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    21241 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    21301 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    21361 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    21421 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    21481 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    21541 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    21601 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    21661 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    21721 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    21781 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaatggt
    21841 ggcgaaatca gtgatttaat caatgatatt gtagatcatc aaactgatat gattactggt
    21901 caaaatggtg gttacgtttt attagatcct aaagaagccc ctagtcgtat tttgattatg
    21961 gacacaccag ataagaatac tgcacggaac gttttgcaaa tcaacaacgc tggtattggt
    22021 ttctctaaaa ctggcattaa tggaacgtat gacaccgcat ggacgttaga tggcggattc
    22081 aatgcctcgt ttattacagc tggtgagata gtagggatta ctattagagg tactacatta
    22141 attagtgatg gcactgatta tagaacaagt atagctaatg gcaaaatgac ttggtactca
    22201 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    22261 ggcattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    22321 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttttatc ttttggtgcg
    22381 ccaaatttca gatacagtaa tgctagttat ataaatgatg gtgacggtaa ttctttaggt
    22441 attgatggta gtgcaggtaa ttcatgggaa tttaaagtag ctggtaggac tatgaaattt
    22501 actagtgacg gtatgctaac gttgccaggt tgtttttttg gttcatggga agatgggaaa
    22561 cttgctagat ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    22621 aaaaactcaa ccgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    22681 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgatgatgg taaagttcgt
    22741 gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg
    22801 acacctacag aactagtttt gtgtgctgtt actcatgaag atgttgacca tttcatcatt
    22861 gaaactagta agccaaacgt attggttaga tggaatttag tggcacatcg actagggtat
    22921 gaagatgtta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    22981 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    23041 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    23101 tcacggtgac tgttaaaccg tttttaaatg gcttgcagat ggatacgagc ggcggaacat
    23161 ttactttaaa aggaactacg ccatctaacc gttacgtaga tagtgtcgct actagtgtaa
    23221 ctagtgaaga agtcacattt tctcttgatg gcacatttat gagtgaagca gggtattata
    23281 aacactgcta tgtagaatat agaaaagacg atcaaatttt aacgacgcaa gatatcattt
    23341 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatat gtttcgcaat
    23401 tagaagaatt gattcgaaag tataacgaaa cttttgatgc ttttctggct gaaattaaag
    23461 gtagaatgga tagcttaaat aaacagatta ctgatttaac tggtcaagct aaaacactac
    23521 aagacaagtt agatgctctg aaagaagaaa tttctaaatt aggtaactta catgtgatgt
    23581 actccaacag catcgacttc gggggctatg attatagtgg gaatccgaat ttaatgtcca
    23641 aactaaaatc gagcgatttt aacgttggtt accacgggtc actaactaat gttaacggaa
    23701 tgttacattt tatgtctgat ggtacagggg acattgatat gtttacgcgt attaatacac
    23761 ctcagcttgc tagtgggaaa acctatactc tgagtgcgaa agttcgattt gatgaaggaa
    23821 ctacaggagc tattgataaa ttacgtttgg tgtatcgtac atcaccagga gaaaagatat
    23881 tattggaagc aaataataca actattacaa tagatgatgt agggaaagaa ataacaatca
    23941 aaggcacagc taacgttaat tatcaaatca caaatttaga tcgtttttat attagtgtta
    24001 gtttttctaa cagggataaa ataaatggtg gatttaaatt gtacgacatc aaaatcgaag
    24061 aaggctcaac agccactccg tatcagccaa acttactaga tgacccttac tgggtaggta
    24121 aaacatcgtt gggtaagaat attgcaaata aaggtacgac gttcccaatt aaatctagcg
    24181 cgtacgaaat atataaaggt aacacggaag aagagcttat aataggtcaa acgtatacta
    24241 tcacgcttaa aggaacaaaa cccgcaagtc aaacctttgt agcatataat tattggaacg
    24301 ttaattttgg agatctaaaa ccagttgagg gattgacaga cgtatggtct ctaacattca
    24361 caccaacgaa acttgaaccg ggtttgccta aagaccttcg catttttcag tcgcctaaag
    24421 aaacggccgg cgcatgccga attgactggc tcaagatcga aaaaggcgac acacgaaccc
    24481 cgaatattga gcaatataaa taccgaggaa tcggcatgcg agactcaaac aatccaaaag
    24541 attatgtatg ggatctagaa cctagctatg ttgagaataa cctagctaca gatatcaagg
    24601 ttgctgaaat cacaggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt
    24661 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag
    24721 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    24781 atacagctac taccacatta acagttacaa acgggaatac cggatcagca aagctttatc
    24841 gtgaaggaaa aacagtttct atatattttg tggctttaaa cggaaaaagc agtggtggaa
    24901 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    24961 tggttggctc
//
LOCUS       DAYYAO010000038        24259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000038 DAYYAO010000000
VERSION     DAYYAO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.040"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACHARI_001457"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACHARI_001457"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8442509.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACHARI_001458"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACHARI_001458"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8442510.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACHARI_001459"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACHARI_001459"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8442511.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACHARI_001460"
     CDS             complement(3374..4681)
                     /locus_tag="ACHARI_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8442512.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACHARI_001461"
     CDS             complement(4684..5265)
                     /locus_tag="ACHARI_001461"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8442513.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACHARI_001462"
     CDS             complement(5837..7129)
                     /locus_tag="ACHARI_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8442514.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACHARI_001463"
     CDS             complement(7126..7749)
                     /locus_tag="ACHARI_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442515.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACHARI_001464"
     CDS             complement(7752..8588)
                     /locus_tag="ACHARI_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8442516.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACHARI_001465"
     CDS             complement(8605..9492)
                     /locus_tag="ACHARI_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8442517.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACHARI_001466"
     CDS             complement(9508..10791)
                     /locus_tag="ACHARI_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8442518.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACHARI_001467"
     CDS             complement(11474..12157)
                     /locus_tag="ACHARI_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8442519.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACHARI_001468"
     CDS             complement(12175..12792)
                     /locus_tag="ACHARI_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8442520.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACHARI_001469"
     CDS             complement(12802..13626)
                     /locus_tag="ACHARI_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8442521.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACHARI_001470"
     CDS             complement(13619..14179)
                     /locus_tag="ACHARI_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442522.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACHARI_001471"
     CDS             14353..15309
                     /locus_tag="ACHARI_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8442523.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACHARI_001472"
     CDS             complement(15614..16570)
                     /locus_tag="ACHARI_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8442524.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACHARI_001473"
     CDS             16674..17276
                     /locus_tag="ACHARI_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8442525.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACHARI_001474"
     CDS             complement(17364..18863)
                     /locus_tag="ACHARI_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8442526.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACHARI_001475"
     CDS             complement(18879..19403)
                     /locus_tag="ACHARI_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8442527.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACHARI_001476"
     CDS             19701..20450
                     /locus_tag="ACHARI_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8442528.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACHARI_001477"
     CDS             20441..21226
                     /locus_tag="ACHARI_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8442529.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21912
                     /locus_tag="ACHARI_001478"
                     /pseudo
     CDS             21195..21912
                     /locus_tag="ACHARI_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(22014..23033)
                     /locus_tag="ACHARI_001479"
     CDS             complement(22014..23033)
                     /locus_tag="ACHARI_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8442530.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23052..24053)
                     /locus_tag="ACHARI_001480"
     CDS             complement(23052..24053)
                     /locus_tag="ACHARI_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8442531.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5222 c         4225 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaaacg cagatctgct gctgcttgat
    21361 gtatacatgc ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat
    21421 gatatcccag tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc
    21481 cgctacggtg ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg
    21541 atcgaaaagt atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa
    21601 ttgatcgatc aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat
    21661 ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa
    21721 gatactattt tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc
    21781 aagaaatatt tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg
    21841 gaaatcggcc ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca
    21901 gattactgtt gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca
    21961 actgtttact tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt
    22021 aattgacggg ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca
    22081 cgaaagttct cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct
    22141 acatgcgggg ttacaagaac tctagggtac atttctacta attgctggat agcagggtct
    22201 ggaatcgtct cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca
    22261 tcggttgcaa aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct
    22321 tgaagctctc cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat
    22381 ccttcattga tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata
    22441 tcgcttttgg ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct
    22501 gtctgatgaa tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc
    22561 gtcaacccta tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta
    22621 aacatttgct catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta
    22681 tttctcaaaa gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac
    22741 gatggtacac gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg
    22801 actcccacag tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg
    22861 gtctcatctg ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg
    22921 atattgtcat gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat
    22981 ttttcaaaaa aatctatctc gttcggacga acaccgtaac atgcgattct catactttct
    23041 tcctccattt cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat
    23101 acaggtgtca gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt
    23161 gcctccctat caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc
    23221 atgataacga ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa
    23281 actgctagaa tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac
    23341 catgaagttt tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt
    23401 gctagccacg ccaatggtga acttaaacca gctaaataat ccatcggctg aaatagccac
    23461 ggtgcagtct ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta
    23521 actgacactt gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg
    23581 ataatgattg gattgacgaa catcgctttg atatttttag cttccatctt caagccactc
    23641 atttttatgt atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa
    23701 tacatcacgc cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta
    23761 gaaccaaaga tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa
    23821 aaaggttttg aaactagaat aagaataata taaataacta atccccaaat caagacattg
    23881 attccttgca tcagtaattc tgtattgata tcttgcatga aagctcgaaa tgccagtgct
    23941 ggcaaagcca ctgttaagac gactttcgat agaatttttc caacagattc agaaaaaatc
    24001 cccttttttc tacaaacaaa ccctaataat ataataaaaa ctgttgaagt gattgcactg
    24061 acaatcgaca tatctgtcaa agtcgatcga aaaatgtcaa ataagttcat ctttctcctc
    24121 ctaattatgt tcatttattc acaataaaaa aagcttttat tttgctaata cgtattaaca
    24181 tttttaattt tatctttaca ttgttactta ttgaacaata ggaaattaaa gaaacttttg
    24241 aaacttatgt aaagtgatt
//
LOCUS       DAYYAO010000039        24258 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000039 DAYYAO010000000
VERSION     DAYYAO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.041"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..996
                     /locus_tag="ACHARI_001481"
     CDS             139..996
                     /locus_tag="ACHARI_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8442532.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1111..2031
                     /gene="pstC"
                     /locus_tag="ACHARI_001482"
     CDS             1111..2031
                     /gene="pstC"
                     /locus_tag="ACHARI_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8442533.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2031..2915
                     /gene="pstA"
                     /locus_tag="ACHARI_001483"
     CDS             2031..2915
                     /gene="pstA"
                     /locus_tag="ACHARI_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8442534.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2930..3736
                     /gene="pstB"
                     /locus_tag="ACHARI_001484"
     CDS             2930..3736
                     /gene="pstB"
                     /locus_tag="ACHARI_001484"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8442535.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3752..4510
                     /gene="pstB"
                     /locus_tag="ACHARI_001485"
     CDS             3752..4510
                     /gene="pstB"
                     /locus_tag="ACHARI_001485"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8442536.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4523..5200
                     /gene="phoU"
                     /locus_tag="ACHARI_001486"
     CDS             4523..5200
                     /gene="phoU"
                     /locus_tag="ACHARI_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8442537.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5438..5737
                     /locus_tag="ACHARI_001487"
     CDS             5438..5737
                     /locus_tag="ACHARI_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442538.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5778..6470
                     /locus_tag="ACHARI_001488"
     CDS             5778..6470
                     /locus_tag="ACHARI_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8442539.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6467..7234
                     /locus_tag="ACHARI_001489"
     CDS             6467..7234
                     /locus_tag="ACHARI_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8442540.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7334..8902
                     /gene="liaX"
                     /locus_tag="ACHARI_001490"
     CDS             7334..8902
                     /gene="liaX"
                     /locus_tag="ACHARI_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8442541.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9199..9519
                     /locus_tag="ACHARI_001491"
     CDS             9199..9519
                     /locus_tag="ACHARI_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8442542.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9519..9869
                     /locus_tag="ACHARI_001492"
     CDS             9519..9869
                     /locus_tag="ACHARI_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8442543.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10036..10974
                     /gene="hprK"
                     /locus_tag="ACHARI_001493"
     CDS             10036..10974
                     /gene="hprK"
                     /locus_tag="ACHARI_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8442544.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10989..11819
                     /gene="lgt"
                     /locus_tag="ACHARI_001494"
     CDS             10989..11819
                     /gene="lgt"
                     /locus_tag="ACHARI_001494"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8442545.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11863..12888
                     /locus_tag="ACHARI_001495"
     CDS             11863..12888
                     /locus_tag="ACHARI_001495"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8442546.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12903..13841
                     /gene="galU"
                     /locus_tag="ACHARI_001496"
     CDS             12903..13841
                     /gene="galU"
                     /locus_tag="ACHARI_001496"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8442547.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13966..14406
                     /locus_tag="ACHARI_001497"
     CDS             13966..14406
                     /locus_tag="ACHARI_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8442548.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14409..15011
                     /locus_tag="ACHARI_001498"
     CDS             14409..15011
                     /locus_tag="ACHARI_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8442549.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15079..16185)
                     /locus_tag="ACHARI_001499"
     CDS             complement(15079..16185)
                     /locus_tag="ACHARI_001499"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8442550.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16395..17396
                     /gene="ccpA"
                     /locus_tag="ACHARI_001500"
     CDS             16395..17396
                     /gene="ccpA"
                     /locus_tag="ACHARI_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8442551.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17732..20077)
                     /locus_tag="ACHARI_001501"
     CDS             complement(17732..20077)
                     /locus_tag="ACHARI_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8442552.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20356..21258
                     /locus_tag="ACHARI_001502"
     CDS             20356..21258
                     /locus_tag="ACHARI_001502"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8442553.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21471..23246
                     /locus_tag="ACHARI_001503"
     CDS             21471..23246
                     /locus_tag="ACHARI_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8442554.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23503..>24258
                     /locus_tag="ACHARI_001504"
     CDS             23503..>24258
                     /locus_tag="ACHARI_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8442555.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         7750 a         4334 c         5088 g         7086 t
ORIGIN      
        1 atagcataga aacttttaat gagatagttt tttttacaat tatgagaatt tattttgtta
       61 tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag
      121 gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat aggattagcc
      181 attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc
      241 tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct
      301 ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca
      361 ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc
      421 tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat
      481 attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa
      541 aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga
      601 agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca
      661 caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc
      721 agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta
      781 gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg
      841 tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat
      901 gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc
      961 gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt
     1021 agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt
     1081 atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca
     1141 aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg
     1201 ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc
     1261 ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc
     1321 caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt
     1381 attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc
     1441 atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt
     1501 gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc
     1561 agaagtattt ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt
     1621 atgatcttgc cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac
     1681 tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg
     1741 ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt
     1801 ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt
     1861 actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg
     1921 ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta
     1981 ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa
     2041 aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga
     2101 ttttagcagc tttactactt tatatcttag ttcggggaat accgcatatt tcatggtcct
     2161 tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta
     2221 actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg
     2281 gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga
     2341 ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg
     2401 ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca
     2461 acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac
     2521 gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc
     2581 cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg
     2641 aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact
     2701 ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg
     2761 tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg
     2821 gcgcttctgc cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg
     2881 gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata
     2941 taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac
     3001 ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt
     3061 tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct
     3121 tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat
     3181 gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat
     3241 tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt
     3301 tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag
     3361 cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc
     3421 tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga
     3481 tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga
     3541 agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca
     3601 acaagcagct cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata
     3661 tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc
     3721 cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga
     3781 tcttcatctg tattatggaa agaaagaagc actaaaaggg atcaatctaa gtttcaatca
     3841 aggggaaatc actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc
     3901 tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa
     3961 agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat
     4021 ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt
     4081 gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa
     4141 ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc
     4201 cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat
     4261 cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat
     4321 gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc
     4381 ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg aatacaacga
     4441 tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg
     4501 atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct
     4561 ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg
     4621 tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat
     4681 caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc
     4741 agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg
     4801 tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag
     4861 aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga
     4921 caacgtccta gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat
     4981 ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca
     5041 ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga
     5101 acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa
     5161 aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc
     5221 gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag
     5281 taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta
     5341 ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc
     5401 acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat
     5461 cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag ctgctttata
     5521 tgaagtacct gtcatcgaga tcgaaaaagt acgaataaaa gaggatcatc aattgattac
     5581 aggaagactg atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca
     5641 aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca
     5701 tggagatgaa tggcagctag tagcaaaaaa aaaatgatgg ctggcttttc ttcttcacaa
     5761 gtatctccat tggcgccatg ttgcttattg gcggaaaaca gggtctattt gctttaggaa
     5821 gtctcatcat caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag
     5881 ggatttctct attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat
     5941 tggctttaga tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg
     6001 tttttttagc attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc
     6061 gctatgagga actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac
     6121 tgattggaac agctggagga acactcgata ccgttatcac agtcattgcg acattagaag
     6181 aattgactaa gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag
     6241 tgggtcgaga tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt
     6301 cagttccatc gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac
     6361 agcatttatc attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga
     6421 tccctatctc ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat
     6481 tatacttgtt gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta
     6541 tattctttta ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca
     6601 tcagaagtgg ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat
     6661 cttatttttt atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat
     6721 ctttttattt tgtttcctac tattgcttcc tatcactgat tttttagcga tccaaggttt
     6781 tacatcggta gaactagaag aattatctgg cttagataag actttagcga ttgattttag
     6841 attacttacg agatcgttgc ttctgattag tcttagtggt gctgttttag atgctagtgt
     6901 agcaatttct agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca
     6961 attgcgccat tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact
     7021 tctttttgca ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat
     7081 tccttttcag caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct
     7141 taccacattg tctgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt
     7201 tacaaaaaag aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat
     7261 ttttactgaa tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa
     7321 ggaggcaatt tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct
     7381 ttcgactgaa gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca
     7441 aataaaaaaa gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca
     7501 attgatcgat aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga
     7561 aagagaaagg caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc
     7621 gaataaaact tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc
     7681 acgcaatgag aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt
     7741 gacagaagaa gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc
     7801 tagtggcgat acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg
     7861 taagaatcaa tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga
     7921 ttggaaagat caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg
     7981 atcacagtta ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga
     8041 caacatggaa tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga
     8101 gcatgaattt tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg
     8161 aaacgtgacg ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact
     8221 ttacggaaaa atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt
     8281 taatgaagat catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt
     8341 ctacttacct aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat
     8401 gattgaaaca ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt
     8461 ggaccaatta actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg
     8521 aaacgggaac atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc
     8581 gacagcagaa aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga
     8641 agataatttg aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc
     8701 agatggcatc ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct
     8761 gtccaattac gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg
     8821 tcgtgtttca gatggtgaaa tcacgcaagt gcaactttca acgacaacag gaagtatcta
     8881 tttaaaagat acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag
     8941 aagcgatttt gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgataggc
     9001 tatctcttga acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa
     9061 ggaataattt cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat
     9121 tttgcagaaa gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata
     9181 gtagaacaga ggtgagacat gatgaaaaaa aagttgacaa aatccaataa aaatgttgtt
     9241 ttgacaggga ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt
     9301 gtgctttatg tattcctgag cttagttgct tttggaagtc caatcatttt atacatttta
     9361 ttagctttga ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca
     9421 tattatgaaa aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa
     9481 gcggaaaaaa tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt
     9541 tagttaatac gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga
     9601 gcagtatttg gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca
     9661 aaccgatttt gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt
     9721 ttgttatcaa tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta
     9781 gtttttcttc attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa
     9841 ttgtcagtga acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt
     9901 tttttattta gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt
     9961 ttttaattgg tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga
    10021 aatgaggtag catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct
    10081 ttggaaatcg tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc
    10141 tcacgcccag gactagagtt gactggttat tttaattatt attcccataa tcgtttacag
    10201 ctatttggca gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc
    10261 atcatgagaa gattatgtag tgaagatatg cccgcattta ttatttcaag aggattaccg
    10321 gctccagaag aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc
    10381 atctcaactt ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct
    10441 cgaacaagtg tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt
    10501 gacagtggga taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg tcatcgattg
    10561 atcgcagacg atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg
    10621 cctaaaatcc tggaacatct aattgaaatc cgaggaatcg ggatcattga tgtaatgaat
    10681 ctttttggtg cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa
    10741 gcatgggcaa aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc
    10801 gcggaagtgg gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc
    10861 atcattgaag tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa
    10921 acatttgaag aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga
    10981 gtgacaatat gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct
    11041 actggtatgc attgatcatc gtatcaggta tcattatcgc catgtggctt agcagcagag
    11101 aggctgttcg ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc
    11161 ctatctccat tattggtgct cgcttatatt atgttttatt tgatcttccc cagtatatag
    11221 ctgatccaat ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga
    11281 ttgcaggcgc gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt
    11341 ttttagatat cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt
    11401 tcatgaacca cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt
    11461 taccgcaatt catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc
    11521 tttatgaatc actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac
    11581 cacacctttt gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc
    11641 gtttctttat tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt
    11701 ctcaattatt atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa
    11761 aaaaaggaaa tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat
    11821 ttttagagcg ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg
    11881 gttttaggac caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat
    11941 gacgttcgga tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg
    12001 aatcaacgtt atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg
    12061 gaagatgcag taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt
    12121 tctgttgcaa aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt
    12181 aaaggattag aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca
    12241 gcagaaaaaa gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagcg
    12301 aaacatgaca tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta
    12361 caagagctgt tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta
    12421 gagatggggg cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta
    12481 gggtttggcg ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt
    12541 ttaggtgtag caatgggtgc caacccgctg acctttatcg gattaagcgg agtaggcgat
    12601 ctagtcgtta cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga
    12661 caagggcagc ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat
    12721 actactcaag cagcgatgga actagcccaa ataatggaaa tcgaaatgcc catcactcaa
    12781 acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg
    12841 ttacgtgatg ggaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt
    12901 atatgaaagt caaaaaagca gttattccag cggcaggatt aggaacaaga tttttaccag
    12961 caacaaaagc tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca
    13021 tcgttgaaga agcgttgaaa tcaggaatcg aagacatctt gatcgttaca ggaaaagcta
    13081 aacgaccaat cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa
    13141 aaggaaagac cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca
    13201 tccgtcaatc tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg
    13261 gaaatgaacc gtttgtcgtt atgctaggcg atgatatcat ggaagatgaa gtaccattga
    13321 cacagcaatt aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag
    13381 ttcctcatga tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg
    13441 ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt
    13501 tagctattat tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac
    13561 aaccaggagc cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac
    13621 aacgcgtatt tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt
    13681 ttatgaaaac aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg
    13741 agtatatcct gtctttaggt aaaaaattag aagcagaagg aagcaagatc gatcataatc
    13801 caaaaggaat aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct
    13861 tcagctgcta attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga
    13921 aaatgctatc cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa
    13981 attgcaggat tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg
    14041 ttgttgcaag tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg
    14101 acgatcgaaa tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttgctg
    14161 gtcaaaagta atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg
    14221 ttcacagcag ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac
    14281 atcgcgacga aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa
    14341 gtcggtggga cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga
    14401 aaataaaaat ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggaacagccg
    14461 cagcaatcgc cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag
    14521 ctgcccaagc ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa
    14581 aatcgactga gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag
    14641 gtgatctagc aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg
    14701 aatctgacga tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca
    14761 aaaaaacggc agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata
    14821 cttcagaaga tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca
    14881 atgaaggaat gtcagaagca aaaccagttg cagatgacac aaaagaaatg atgaaaatgg
    14941 caaaagaaga tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta
    15001 caaatcacta aaatcactaa aatcacaaat ttatagaata aaaaggtggg agcggactag
    15061 ctctcacctt ttttactctt atttttctaa aactagtaat tcttttggtg tagtcgtgaa
    15121 tggcacacag ccatcgcttg tcacatatac acagtcttcg atacgtaccc ctactttttc
    15181 aggaatatag attcctggtt caagagagaa gcacatccct tcttcgatta ctaagtcatt
    15241 tccttctaca agggaaggga attcgtggac ggttgttccg atcccgtgac ctaaacggtg
    15301 attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc
    15361 acctgctgtc acacctggac ggacggcttc ttgtgcggca agctgtgctt caagagtgat
    15421 gttgtatatt ttttcttgga agtcggttgg tttttgataa gcaattgtac gagtagcatc
    15481 actgcaatag ccattccaaa caacgccaag atcgaataac acaagttcat tcggtgaaat
    15541 cttcgtgttt cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt
    15601 gtcgaaagac atttgtgaaa caccttgttt tttcaattgg tattcgattt ctgcaatgat
    15661 cgcttgttca gtcacgcctt ctttcactgc tttaaagcca atctcaaatg caacatcggc
    15721 ccatttacca gcttcgatca gtttgtcttt ttcctctaga gttttgatca gttgtagacg
    15781 ttggatgatg gaagctagat cagtgtaaga atccgttccc gtcaagatat cagagatagc
    15841 atcgaagcgc tcgacagtca aggcgttctt ttcaatccct acattgccag gctgaccata
    15901 gcgagcacga agttcttttc cgatgatctc ccaagggttt tcgctgtcta aatagccacg
    15961 gacatcatac gcccaactac tgttttgagc atcttcaact tccaaagcag gagtgaaaag
    16021 gaaaggatct tgttcaagag ggataaaaag agctagtaca cgctcatgag gatcactttc
    16081 gtatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt cgacttggtg
    16141 ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt
    16201 ctaaattaat tttgtattca ataattgtac cactgattta ttaaaagacc aacgttctaa
    16261 aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca attcttgaga
    16321 ttatttgata ttctatttta gaaatgtaag acgtattttc agaaaacata taaaatatga
    16381 ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa
    16441 atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtc aaaccagcaa
    16501 caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaac gcagttgcgc
    16561 gtggcttggc aagtaagaaa acaacaacag ttggggtaat catccctgat gtcagcaata
    16621 tgttctttgc ttctttagca cgtggtatcg atgatgtggc aacaatgtac aaatacaata
    16681 ttatcttagc aaattcagat ggtaacgacc aaaaagaagt caatgtattg aataacttac
    16741 tggctaagca agtggacggc gtgatcttca tgggtcacca tatcacagac gaaatccgtg
    16801 gagaattttc acgttcaaaa actcctgttg tattagctgg ctctattgat ccagatgaac
    16861 aagtaggtag cgtaaacatt gattatgtat cagcaacaaa agatgcagta aacaaattgg
    16921 caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc gttgatcgat cctatcaatg
    16981 gacaaaatcg tctgaaaggc tataaagaag cattaagcga aaataaccta ccatatagcg
    17041 aaggattgat ctttgaagca caatacaact tcaaagatgg tctatctcta gtcgatcgga
    17101 tccataatag cggtgcaaca gctgtctatg tttcagatga tgaattagcc atcggtattt
    17161 tggatggttt attagaccgt ggcgtgaaag taccggaaga attcgaaatc atcacaagta
    17221 acaattcact actgacagaa gtagctcgtc ctcgtttaac aagtatcaca caaccattat
    17281 acgatttagg ggcggtatct atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg
    17341 aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatctaca aaataataaa
    17401 aggacaacca ttcagaggtt gtgagcctgc cgtttaactc tgcgttacaa ggagcaattt
    17461 caaaaaacag catttcatgt ttgatgaata ttgtgactcg tgattcggag ttggcaggag
    17521 aacaccgttt attcgggaaa aggggttatg acaaaatttt gtttaatcct ttttttttgt
    17581 cttaaaaaag agagaaagtg gttgaaatta catttatcgt atataaaaca atccgtgtat
    17641 agatgctgca ttatgttcac caccatcaaa aaaataaggg actatgacaa tactattgtc
    17701 acagtccctt attactgtgt attctattaa atcaatcatc tttctcatct gattctttgg
    17761 ttgcttgatt cgctcttgca taagctgaag cggtgttcca gtaatctgta tagttttcat
    17821 cggtaccgcc aacgccaaat ttaaaggcgc ttttcttcgg tccatttttc gcccatagcg
    17881 attcaactgt ttttcctggt gtttggatgg attttttatt gacagtaact tttccaggtg
    17941 tttgaccagt aaatccagat acttttttct tcatcacatc agatgataat tcgaatttct
    18001 catcagtgcc aaaaatgtca ggttttgctt ggtaagcttg attgatcaga ttggcaagat
    18061 aattagccga acgtatcctt gctttgctgt ccattccagt attgtcatcg tgccctgccc
    18121 agctactgat ggtaatagac ggagtagaga caattagcca tgaatctcgg tattcattgg
    18181 tagaaccagt ttttccgacc caatctgctt ttcctagatt tccgtttaag cttgagatgg
    18241 catctttgaa tggtgtagtg atttttgcat tgatcacact gcgcatcatg tcattcatga
    18301 tcgatgccgt tgcttccgaa tagatccgga cagagttgct ttcgtgttta taaattacat
    18361 ttcctgcgtt atctgtgatg gaatcgatga tatagccttc ttcatagacg ccgccgtttg
    18421 ctaaagcctg aaatccattg gtctgttgaa gcgtcgtgac atctactgta cctaaaggag
    18481 cagattcaac gccccaattg tcatttgccg ggtagttcat ctttgccaag tagtgctcgt
    18541 aagcatattg tttcgatcca ccggaattca acacatcttg atataagtga taagccggga
    18601 tattgttgga ccattctagt gactcacgga cggtttggaa ggtgttcgat ccttcgttag
    18661 tagcattgac gattttctcg ccagcatttt ctccttcttg ccaagttgtt gcataatctg
    18721 atacacggga ttctgatccg ataagcccca tgtcgattgc aggtccataa acaagaactg
    18781 gtttgattga tgagccagct tgacgttctg tatcgaaggc atggttattt tgattctgac
    18841 tgtaattccg tccgccaacg aatccgtaga tgcggccagt tctgttatcc atcaatacat
    18901 ttccggtttc tacttgactg ccagcaccat catctaacat gtatccataa ttttctacgc
    18961 cattttgcaa tgcagcatag acatctttat cgattgtcga atggattgta tatcctttat
    19021 tttggatcgt ttgctgtgcc ttttgataat aagcgtcact gaccgatgaa tcagacatat
    19081 cagcgttgtc tttttcagcc aattgttgtg caatgattct tgttgcttca ttcatcacag
    19141 tataatacaa gaattctctg tcagtctgtt ctgcgatttg ttgtggtaga aagtcttttg
    19201 taaggtcata ggcttttgct tcttcgtatt gttctttcgt aatttggcct tcacgataca
    19261 tactgaataa aacaaaatct tttctggcaa ggccggcaga aagatcttct tttagcgcac
    19321 ctgtgttcgt gtatggacta tacgtgatcg ggctttgggg taatccagcg atataagcag
    19381 cttgcggcag cgtcacatct tttgcatcta caccaaagat tcctcttgca gcttcctgga
    19441 ctccagcaat gttttgtccc ttattattac gaccaaatgg tgagacattc aaatacgtag
    19501 caatgatctc atcttttgaa aagtattttt ctacttgtgc agaaagaatg atctcgtttg
    19561 ctttacgttt gaatgttgtt tcatctgtaa ggatctgctg tttgactaat tgctgtgtca
    19621 aagttgatcc accagaagag ccaataccag tagcttcaga tactaatgct cgtaaaacag
    19681 ctttaggaac gtaaccttgg tgtttatcga agtattcatc ctctgtgctg ataattgcag
    19741 ttttcaataa aggtgaaatt ttatcagatg aaatagtcgt gcgcatcaga tctgtctgga
    19801 tttttgatat ctctgtatta tctgcataaa caatctttga cgtttctgta atgtttccta
    19861 gttctgtctg cagtgctttt ttagtcggca attgtgtgtc ttcgacgaga taggcaaaat
    19921 agccagcgcc tattccaata cctagagctg cgaacatcaa aagaagaaca gtaataaaaa
    19981 cagtcaatga ttgaaaaact ctaaaaataa tcttaggaac aaaccatttt tctttttttc
    20041 ttctattctg tttttttcga gtatttttgc gtggcaaaag ggtcacctca actaatggtt
    20101 ttatttcgct aattatagca aataatagaa caaaaaaaca gacaaggctg tttttggcag
    20161 gaatttcttt tttaaaagag ttaaaacgtg agaaaatgat cttttttata aaagagttct
    20221 taagcttttc tttagagaaa atttcagtta ctatggagaa cagggcaaag aaaaatcaat
    20281 cattttcttt gtatatcatc ttaccaatat ataggaattg ttagtaacaa ttgcctttca
    20341 aggaggaatc catccatgtt attaggctca cacgtcagca tgagcggaaa gaaaatgctg
    20401 ttaggagcag cagaagaagc agccagttat ggatcttcga cttttatgat ctatacaggc
    20461 gcccctcaaa acactagaag aaaagccatc gaagagatga atattgatgc cggaaaagcg
    20521 tacatggcag aaaacggtct aacaaatatc gtcgttcatg ccccttatat cattaatttg
    20581 gggaatacga tcaaaccaga aatgtttcct tttgccgtcc aatttctgag agaagagatt
    20641 gttcgtgcgg aagctttagg cgctactcaa attacacttc acccaggtgc tcatgtaggg
    20701 gcaggaacag aagccggaat tgctagaatt attgaaggct tgaatgaagt tttgacaaaa
    20761 gaacagacgg cacaaatcgc tttggagaca atggcaggta aaggtacgga aatcggtcga
    20821 acatttgagg aattagcgca aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt
    20881 acttttgaca cctgtcatac gaatgatgcc ggttataata ttaaagaaga cttttcaggt
    20941 gtaatggaag agtttgataa gatcatcggg ttagatcggc ttaaagtact ccatatcaat
    21001 gattctaaaa atccgcaagg ttcgcataaa gaccggcacg ccaatattgg ttttggaaca
    21061 atcggattcg acacgctgaa taagatcgtc catgatccaa tgtttgacaa tatttcgaaa
    21121 atcttggaaa ctccctatgt cggcaaagat aaaaagaaag cttacgcacc atatggaaaa
    21181 gaaatcgcca tgttcaaagc acaaatattc gatccagaag tgtttcctga gttattagca
    21241 gaagaagtga tactctaaga ttttctatct gacagcagca aaaaaggacc aggcacattg
    21301 ccttgtcctt ttttgtttaa gaaacaaagg agtgcttttt gcagtctttt tcatcatgca
    21361 tcttctcccc cctcttttta aatttagaca aacagggtat actggtagaa aagtaaaaaa
    21421 acgaaaagtt tcacaagcaa gtggcaaaac gtgattggaa ggacgaaaag atgctgaaca
    21481 ctttaaaacc cttgatcaac caactaaaaa agaaacatac atactttatt cttggcatag
    21541 ccctattatt tacaattatt cgtttgtatt tatatagtaa agcaatctat ggaacagaca
    21601 tgtcggcaac ctatgatgac cagctgctga ttcgctatgc tcaaaatatt ttggacggta
    21661 aatggctagg ggagtatacg actacaacac tttccaaagg aatctcctac agcttatttt
    21721 tagtacttgc acataaactg catctttctt atgcagtttt gttcggcatg ttcagtattt
    21781 ttgccagtat attggttgca ctcgcgttta aaccgatcgt gaagaacaag tatcttcttt
    21841 taagtttcta tttttatttt ttgttttctc cgattagttt tacacatgaa tattccactc
    21901 gaacgtatcg taatgccctc gtgattccgg ctgttgagat cattatcgct tgtttactag
    21961 ctatttatta ccgtaaatat agttccagca aaaaaatgct gccgtggttt ttagggctag
    22021 ctgtttcttt tcctttcttt tggttcattc gcgaagattc catctggctt cttcctttta
    22081 ctgtagtcgc gttaggcatc agtttcgtcc agatcttgct aggacaagac ttttctttca
    22141 aagaagcaaa gaagttaaaa gaacgaccat ttctcctttc gaaacaagaa tggctgaaac
    22201 tttcattgtt tttactccct ctagtcggtt tctccatcgt ctatcagtgg atcgaatata
    22261 aaaacgagac aacatatagt attgccgcca tcaatgaccg ttcgagtggc gaatttggac
    22321 gtttgatgaa acagctgatc cgaatagatg acgaaacgaa cttaaataaa gaaaacagtg
    22381 agatatgggt ctcgcatgct gcattaaata aagcattaga cgcatctcct acatttcatg
    22441 ctgtttctac tcgtattgac gaattgtata cgactcatgc gtggacccaa ggtggaaaag
    22501 taaaagaatt accaggagat atcatttttt gggcattgcg agatgtggta agcgaatctg
    22561 gttattacca aaacaatgca aaagaaacca atgatttttg gaaaaaagtc aatcaagaat
    22621 tagaagaagc ctatcaaaag ggaactatca aaaagaaaaa agaattctat ctgatgggat
    22681 caggggatgg aaagacgatg aatgatctgc caatcgtatt tgcttttctg aaatcaggat
    22741 atgcctatgc atggcgatac aaagatttcc atcaaggggg gaattatagt ttcggtacgc
    22801 aagaacaagt cgaacaggcc caagaattat tgaatcttcc actaatggat aattggatcg
    22861 atagcagtga tccgagaaca cctgatttcc agttgacaaa aacagcaaaa gtcgcgaatg
    22921 tcatgataaa aatttaccaa gttctggcca ttcccgtgct gattgcttca atagcaggga
    22981 tattacttat gatactagga accgcatttg caaaagctga tcgtgcctat tatagttcct
    23041 ctttgattgt agtcaccgga ttaatactga cacaattggt ttttcttttc ggcgtctcgt
    23101 ggttttgttc ttatgcgcca gaaaaaaagg attatttttt aagtgtctac actggcgcag
    23161 gcgtgccatt aacgcaattc atcgtagtaa caagcatgct agttttcagt aaattgaatc
    23221 tgaaaaaagc tttatcttcc aaataaaaac atactaagat tagaggagaa catcattgac
    23281 gaatgatgtt ctcctctatt ttctattttt agattatagt gaagagtgaa agaaagttcg
    23341 aacaaagcct tgaggactat gagtccctat caaaaaccga acttctttac tgtcatttta
    23401 gaatcaggtt tacgtatgtt ggggccatga gcccgtgtat aggaaattgg aatcgaaatg
    23461 aagcagtaaa gttctttcaa aacttaaaaa ggaggaaaac aaatgcctta cattcttgca
    23521 ttagatgttt caatgggaca cagctacagt gtcctataca aagacgacac ctgcttattt
    23581 gaagacgaga tcgaacacac tcaaagagga tttcatgcat tactcgaaga gatccagcac
    23641 ttgcctgaac gtccattgat tgtttttgaa gcaacgggga tttattccag accagtcgaa
    23701 aagttctttc aagatcatca cttttcttac tgtttgttaa acccgttaga agcgaaaaaa
    23761 cagatggaag aaaacacctt atgcagttgg aaaacagata aatcggatgc ccatcggctc
    23821 gcacagactc atcttaaaaa tcaacgtcag ccaaaagaag tacaaagcaa tttttactta
    23881 gagatgcgcg atttagcccg tttttatcaa gaaattgaga agaaaatcac tcgtctgcgc
    23941 atggatttac acaactgcct tcagttgact tttccagagc ttgaacagtt ctttttaaac
    24001 aggctgaccc cttacgcatt gatattgatc cgtctttttc cccatccaga ttttgtattg
    24061 gcatctactc ggacaaaaat aaaaaataaa ctgatcaacg aaacacggaa aaaaatctcc
    24121 gcgaatcgtg ccgagcaaaa ggcagatcaa atcatccact atgcacagtg tgcctatcca
    24181 gctgttgaaa aagacagtat ccactgtcaa aaaacgatct attacgctga acttctccaa
    24241 gatttactcg aacaaaaa
//
LOCUS       DAYYAO010000040        24205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000040 DAYYAO010000000
VERSION     DAYYAO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.042"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..449)
                     /locus_tag="ACHARI_001505"
     CDS             complement(81..449)
                     /locus_tag="ACHARI_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8442556.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            620..1576
                     /locus_tag="ACHARI_001506"
     CDS             620..1576
                     /locus_tag="ACHARI_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8442557.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1649..1765
                     /locus_tag="ACHARI_001507"
     CDS             1649..1765
                     /locus_tag="ACHARI_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8442558.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1820..3502)
                     /gene="recN"
                     /locus_tag="ACHARI_001508"
     CDS             complement(1820..3502)
                     /gene="recN"
                     /locus_tag="ACHARI_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8442559.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3520..3969)
                     /locus_tag="ACHARI_001509"
     CDS             complement(3520..3969)
                     /locus_tag="ACHARI_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8442560.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(4076..4894)
                     /locus_tag="ACHARI_001510"
     CDS             complement(4076..4894)
                     /locus_tag="ACHARI_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8442561.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4903..5793)
                     /locus_tag="ACHARI_001511"
     CDS             complement(4903..5793)
                     /locus_tag="ACHARI_001511"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8442562.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5795..6019)
                     /locus_tag="ACHARI_001512"
     CDS             complement(5795..6019)
                     /locus_tag="ACHARI_001512"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8442563.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(6024..7361)
                     /gene="xseA"
                     /locus_tag="ACHARI_001513"
     CDS             complement(6024..7361)
                     /gene="xseA"
                     /locus_tag="ACHARI_001513"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8442564.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(7362..8216)
                     /locus_tag="ACHARI_001514"
     CDS             complement(7362..8216)
                     /locus_tag="ACHARI_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8442565.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(8313..8762)
                     /gene="nusB"
                     /locus_tag="ACHARI_001515"
     CDS             complement(8313..8762)
                     /gene="nusB"
                     /locus_tag="ACHARI_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8442566.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8755..9180)
                     /locus_tag="ACHARI_001516"
     CDS             complement(8755..9180)
                     /locus_tag="ACHARI_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8442567.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(9426..10490)
                     /locus_tag="ACHARI_001517"
     CDS             complement(9426..10490)
                     /locus_tag="ACHARI_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8442568.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10662..10907)
                     /locus_tag="ACHARI_001518"
     CDS             complement(10662..10907)
                     /locus_tag="ACHARI_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8442569.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(11046..11420)
                     /locus_tag="ACHARI_001519"
     CDS             complement(11046..11420)
                     /locus_tag="ACHARI_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8442570.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11503..12417)
                     /locus_tag="ACHARI_001520"
     CDS             complement(11503..12417)
                     /locus_tag="ACHARI_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8442571.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(12438..13241)
                     /locus_tag="ACHARI_001521"
     CDS             complement(12438..13241)
                     /locus_tag="ACHARI_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8442572.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(13274..14263)
                     /locus_tag="ACHARI_001522"
     CDS             complement(13274..14263)
                     /locus_tag="ACHARI_001522"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8442573.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(14366..14857)
                     /locus_tag="ACHARI_001523"
     CDS             complement(14366..14857)
                     /locus_tag="ACHARI_001523"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8442574.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(15048..17855)
                     /locus_tag="ACHARI_001524"
     CDS             complement(15048..17855)
                     /locus_tag="ACHARI_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8442575.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(18289..18582)
                     /gene="rpmA"
                     /locus_tag="ACHARI_001525"
     CDS             complement(18289..18582)
                     /gene="rpmA"
                     /locus_tag="ACHARI_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8442576.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18595..18939)
                     /locus_tag="ACHARI_001526"
     CDS             complement(18595..18939)
                     /locus_tag="ACHARI_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8442577.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18966..19274)
                     /gene="rplU"
                     /locus_tag="ACHARI_001527"
     CDS             complement(18966..19274)
                     /gene="rplU"
                     /locus_tag="ACHARI_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8442578.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(19294..19370)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19556..20197
                     /locus_tag="ACHARI_001528"
     CDS             19556..20197
                     /locus_tag="ACHARI_001528"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8442579.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(20259..21890)
                     /locus_tag="ACHARI_001529"
     CDS             complement(20259..21890)
                     /locus_tag="ACHARI_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8442580.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(22013..22372)
                     /locus_tag="ACHARI_001530"
     CDS             complement(22013..22372)
                     /locus_tag="ACHARI_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8442581.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(22531..22604)
                     /locus_tag="ACHARI_001531"
     tRNA            complement(22531..22604)
                     /locus_tag="ACHARI_001531"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22568..22570),aa:Arg,seq:tct)
     gene            complement(22913..23596)
                     /locus_tag="ACHARI_001532"
     CDS             complement(22913..23596)
                     /locus_tag="ACHARI_001532"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8442582.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(23724..>24205)
                     /locus_tag="ACHARI_001533"
     CDS             complement(23724..>24205)
                     /locus_tag="ACHARI_001533"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8442583.1"
                     /translation="TLIIFLILILLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIYVR
                     PKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNI
                     IGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT         7082 a         5294 c         4003 g         7826 t
ORIGIN      
        1 ttttttattc ataaacattt ttttaaaaat caagctgcaa aaagcctttt aaaagacttt
       61 ttacagcttt tctatttttt tcataggtaa gaaagtatgt tcacaatgat caagacagcg
      121 tacaagacga ttgtcaacag aaaaaccaaa cgccaagtca tcttgaaata acgcggataa
      181 ataatttctt cataataata tgcttggaaa acagcaatac aaattcccat tagcaaaaca
      241 gaaataaaga aaaacggtaa gatagaaatt gtccctgtat ttctagacaa tgcatgcata
      301 ccgccccaca aaaaaggaat cgccaaatca ggggctttta ttttaaaacg acgatttaac
      361 gaaaataatt ttacaagaaa ttggcaacca aacaaaacga ttaccgggta aatataccaa
      421 aacaagataa gtggtgtgaa tgtgcccatg aacgtaaact ccttttttat cgttgcttca
      481 agtatactgt atatacttta cacaactgtt tcattttatt taaatttatg cagttatatt
      541 ctatatctta caaacacatt ccatgtattc caattcccta tccgttataa taaaagaaac
      601 aggcaacagg aggaaaatca tgatcaaata ttttacatta gataatgaga aactgcgtaa
      661 taccggaaag gaaactgacc aaacgatctg gtattgtgta gaacgtccta atcaagaaga
      721 gattcatcag ctgacacatc aatttgaaat tccgtcagat tacatcactt ccatattgga
      781 cgatgcagaa aattcccgta cagaagaatt caatcaatca aagttgacaa agccagcgtt
      841 attattgtta caatatcctt ttgctaaaac aagtcctagc ggttattttc aagtggatac
      901 atacccgctc tctattattt tgacgcccaa aaagaagctt atcacaatta cgaatcatga
      961 accactgttt ttaaagaaag tctttagcca gacctttcca aaaagtgata tttcgcctaa
     1021 tctcaatatc ttctttcaat tcatttggca ggtcacactc tcttacaaca gttatttagc
     1081 tacgcttcaa tctcaatgcg atactttgga agccaaactt caagtatcca ctgaaaacaa
     1141 acaactttat caaatcatgg atatccaaaa aagcctcgtt tatttcagag aagcgacttc
     1201 tgctaattta gatgcattga aagaactagc gcaaaataaa cttttccaag aaaatcatgc
     1261 tttgaaaaat catttacgtg atgtattggt ggaaacagag caggcaatga ctacttctcg
     1321 cattcaatta aaactagtag aacaaatgaa tcagactttt tcagcaatcg tatcgaataa
     1381 tctgaataat attatgaaaa ttttaacttc tttaacgatt attttaacta taccgacaat
     1441 cgtcggcagt atctatggaa tgaacattaa gttacctatt gctgaaaggg atgatgcctt
     1501 tatatggcta gttctattaa cattatttat ttgtatcgta actacctatt atttaaaaaa
     1561 agggaagttt ttataaaaaa gccctcttgt ggaaatggaa aaaagattgt acacttgaat
     1621 caactaatta atgacgaggt tgaaaacaat gcgtgaaaaa aaacaaacaa gtacttttgc
     1681 taaagtaaca aaatttgtca tttggacaat gctgatttta acgattggat ctgttgtatt
     1741 gactgctgta atgagtgtca tgtaaaaata aaatatagct atcagctgct gaaaaggacc
     1801 tgaaccactg cgaggtggct caggtccttt ttgcgagttt caatagttct tttgcatgtt
     1861 cgagtgtgag ttttgtaatc tcacttccag ccagcatacg tgcaatttct tcttctcttt
     1921 cttttgtttt caactctgaa acagaagttt gcgtacgtcc atcaatcact gctttcacaa
     1981 tataatattg atagtcggcg actgcagcaa cttgaggcag atgagtgatg cacaaaactt
     2041 gagagtattt cgatatttga gaaattttat ctgcgatggc ctgtgcaact cttccgctca
     2101 cacctgtatc cacttcatca aaaataatac ttgtcacacc ttgctcagaa gaaaaaatag
     2161 ttttcaaagc aagcaacatc cgagacagtt cgccaccaga agcgactttc accaaaggct
     2221 tcagtggctc tcctggattg gtcgtaatgt aaaattcgat ttggtcaaat ccatcatgat
     2281 tcaattgtct attttcttct tttaagaaac gtacctcaaa ctcagtgttt tccatataca
     2341 gacttttcaa ttcatgcaga atggattttt ccaattcttt tgcgattttt ttacgcgctt
     2401 catgaagatc tgcggcttgt tgctgaagta gaagctcttt ttgctctagt tcctctttca
     2461 ttttgtccaa ttgaccttct gaaaaatcag aagaagctag ctcttcggtg atttcgtcat
     2521 agtaagccaa tattgactca atggattcgc cgtatttacg tttcaactgt cggattaatt
     2581 ctaaacgttg cgtaacttct tctaaacgac tctcatctaa ctccaataaa tcaatctgac
     2641 gactcatatc gccgattgca tcttgcagca aatagtaagc actttggata ttttcataaa
     2701 ttgcttcata ctccacatct agatgggcga tcccttgcag ttctgaaacg gccaagccaa
     2761 caccatctac actgttttgt tcattattcg tcagtgctcc gtaacctgca gctaatccat
     2821 cagcaatttt ttgataattg ctaagttttt ctctttcttc ttttaatttc tcttcttctc
     2881 cgacttgcaa atcagcagca gctatttctt cttgctggaa atggagcata tctattctct
     2941 gaacatagga tttttcattt tgctgcactt ttcgtaccct tttttccagc tcacgataag
     3001 agatatattc ttgctgataa ctctttttct tctgttggaa agctacatct ccaaaacgat
     3061 ccaataaagc aagatgtgat tcaggctgga gcagttcctg atgttcgttt tgtccttgga
     3121 tatctactaa ataactgccg attcttctca gattagctaa tgtgataata tgaccattca
     3181 ctcgacaaac attttttcca gttaaggaca tatcccgtcg aacaatcaga ttgtcctcat
     3241 ctgtttcgat ccctaattca accatcagtt cagagaatcc ttcttgtttt ggtagttcaa
     3301 acaacccttc aagcacacat ttttctgctc cttgacgtat ataatcgctt gaacctcttc
     3361 ctcctgctaa cagacctaaa gcatcgataa taatggattt acccgcacca gtctctccgg
     3421 tcagcgcggt cattccttcg tgaaaagaaa gccgcaattc cggaataatc gcaaagttag
     3481 taatactgat ttcttgtagc atcacattcg ctcctcaact taaaggtaat gcaggaagtc
     3541 tttcttcaat aaaactgcct ggtcttcagt tcgtgtaatc atcaaaacat tatcgtcatc
     3601 attgataatc gaaaataatt ctttcttgta acgtttatca atcaaatttg ctgctgccga
     3661 agcatttccc ggcaatgttt tcaaaataac aaatttttcc atttggtcaa ctgccacgaa
     3721 agcgtctttt aatagttttt ccaatttagt atttacatct tcgtttgttt cagctggtat
     3781 gctataacgg tagccgccat ctgaagccgg taccttgatc agtttcagtt cctttatatc
     3841 tctggaaatc gtagcttgag taacagatat tcctttgctt ttcaggattt caacaaattc
     3901 ttcctgcttt cggatatctt gctcactcaa caatcgagta atcaaacgat gccgatcttt
     3961 ttttctcatt acgtcttcgc tccttagcat attcttctta atcaatgata gcttatttcc
     4021 ttggtataat aaagaagaat ccgcatactt ctcacttgat tttcacagtt acaatttatt
     4081 tatggtcaaa ctgttcgtgt gcatttgaaa ccacttctgc catattaata gctgaattga
     4141 ttgttccgct ttcgggtact ttcctcaagt gagccaagaa ctcgatattt ccttctcccc
     4201 ccgtgatcgg agaataactt aattccagca catcatatcc accttgtgcc gcaaatgaca
     4261 aaatgtcttc taaaactttt tgatgggtct gagggtcacg aacaattccg tttttcccta
     4321 cagcttctct gcctgcttcg aattggggtt tgattaaagc aatcacttct ccctcttctt
     4381 ttaaaatagc atgtaatgga ggcagcatca gttttagtga aataaaagaa acatcgataa
     4441 cagcaacttc tggaattcct aaggtaaagt cttcgggttt tgcataacga aaattcactc
     4501 tttccattac gacgactcta ggatcttgtc ttattttcca agctagctga ttgtagccaa
     4561 cgtctaaagc ataactcatt tttgcgccat tttgcaaagc tgcatcagta aatccgccag
     4621 tggatgcacc aatatctaac aatgtttttc cttcaacaga tagatcaaat gctttgagtg
     4681 ctttttctaa ttttaatccc cctcgagaaa cataaggaag ttttttacct ttgactctta
     4741 attcactact cactggaatt ttttctcccg gtttatcaaa ccgctcgttt ttttcgttgt
     4801 aaatcaaacc agccatcact gaacgtttgg cttgttctct tgtttcaaat aatccttgtt
     4861 tgaccgctaa tacatctacg cgttcttttt ccattctttc cctcatttct ccacatcaaa
     4921 ttgcttgatc atctggcgca tcagcccgcc atcaaaagaa aaaacgtcct cttctaattt
     4981 gtctatgatc ttgttggcgg ataacagctc aatctccaac gcctcacgtg tcttttctag
     5041 gcctaataaa cgcgggtaag tatttttttc catacgttca tctttaccag ctgttttccc
     5101 taatgttttc gttgtaccaa tcacatcgag tagatcatct cttatctgaa aagctaaacc
     5161 taaatgttcg gctaatcgtt gaagtagcaa taagatttct tcaggttgct tcgctaatat
     5221 gccaccagcc agcaaggcat aacgaatcaa atgccctgtc tttctttcat gaataaatgc
     5281 taattcttct aaagaaagct ttttcgattc gccctcgata tctgcagctt gcccagcaac
     5341 cataccttgt gatcccgcac aaacagctaa ttgctgaagc agcagaagct taggagaatt
     5401 acctaagtgt gccatactga ttagctggaa tgctcctgta agcaaaccat ctccggccaa
     5461 tatagctaat gcttcgccga atactttatg attggtaggt tttcctctac gaagatcatc
     5521 attgtccatc gcaggcaaat catcgtggat caaggaatac gtatgcacca tttctaaagc
     5581 agctgctact tgatagacgg gaacatcaat aggctcatca aaggaagcaa cggccgttaa
     5641 aagtaataag ggacgtattc ttttcccgcc tgctttcact gaataactca tcgcatcata
     5701 gagtcctcta tccgttgtat gctcatctaa aaaagacaag atttcttttt ctacagcagg
     5761 tagatgaata gctgaaaagt cagttaatct catctcagct ttcctcgttt ccgtcaaaat
     5821 caacttcttc atttgattct gtcatcattt tcgtcaaggt ctcttctgct ttagctaatg
     5881 tatcttggca atgtttgctt aattccattc ctcgtttaaa cgaatctaga gctgattcta
     5941 atggaacgtc tccttgttct aactgcatga caattttttc taattcttgc agtgactctt
     6001 caaaagttgg attttccata tttttattct cctttgtcta tctcttgtac ttgtccttta
     6061 acgattccat cttgataatg gatcgtcagc aaatcccctt gatgaatctc ggtgacactt
     6121 tttactactt gatcatttaa cgtcgtatag ctgtagcctc ttcccatgat tttcaatgga
     6181 ctcaacaaat caagagattg aacagcttga gcaaaacgct gctgctgttg tttcatatat
     6241 tgttggattt tctcttctaa gcgtttttct agataagcag tttcttggcg agctgcttta
     6301 actcgataaa gcggagccac ttgttccaat tggtggctta atgcgatcgt ttgtttttct
     6361 ttcgcatgat aaatttgctg tgtagcttga tataatcgct gtttcaactg gtctaatttg
     6421 atggtctgac cttcatacaa tctttcaggc tgccgaaata cataggaacg tcgagcacgc
     6481 tcgaaccgtt cttgtttgcg ctggatctgg cgtacaaacg cctgctgcaa tctagcttgc
     6541 cgttcttgaa tgcgcatcaa ctcttctgac aaaacaggaa cagctaattc tgcagctgct
     6601 gttggagtgg cagcccgaac atctgctact aaatcagcga tcgtggtatc tgtttcatgt
     6661 cctacagatg aaatcaccgg cgtattagct tgataaatcg cccgagcaac ccgctcttca
     6721 ttaaacggcc ataaatcctc aatcgaacca cccccacgac cgatgatcat tgtatcgaaa
     6781 ttgcccatct cttctactct ctggatattc ctgacaacat tgtccgctgc ttggtttcct
     6841 tgtacaactg ttgggaacaa aaccaattgg acaattggat atcttctttt caccgtcgtg
     6901 atgatatcgc ggataaccgc tccgcttgga ctggtaagga ccgcgattcg ctttgggaaa
     6961 cgtggcagca tttttttagg cccttgaaaa aggccttcct tcatcagctt ttcttttcgt
     7021 tcttccaatt cttgatataa agcacctatt ccatcaggct ccatgtgctc tacatagatt
     7081 tgataagaac ctcccgcttc atataatgaa atacgcccaa cgacaaggac tttcattcct
     7141 tcttttggct ggaatcgtaa tttttggaat gctcctttga acataatagc agatattttt
     7201 gcatggtcat cttttaagct gaaatattga tgggcattgg cacgtaaacg gaaattcgat
     7261 atttcgcctg ttaaatatac gcgttctaaa taaggatcgg catcaaattt tcttttcaaa
     7321 tacttcgtta atgtagtgac agttaaatat tcttgtgcca tctattcacc tgctccaaaa
     7381 gcttcggcag attttacggt ttgatacatc agcatcgtga tagtcattgg ccctacaccc
     7441 tttggcacag gagtgatcca gcctgcaata ggctccactt catcaaattt cacatcaccg
     7501 atcagcttac cgttagcatc acgattcatc cctacatcta tcactacagc acctggtttt
     7561 acgaattctt ttgtgacaaa atgtccacgt cctattgcta cgactaaaat atctgcttct
     7621 tgtgccagtt cagccagatt ttcggtttta gaatgagcaa cagtaacagt tgcatctgcc
     7681 atcaagagta gctgagccat cggtttaccg acgatattgc ttcgtccgat aactactgct
     7741 cgtttacccg ccggatcgat agaataagct tcaaacattt tcatgatgcc gtatggagta
     7801 caaggaatct tgtccggttt acccgcaagg agtctgccta aattcatcgg atgaaaaccg
     7861 tctacgtctt tttcagggcg gatagctaac aggatctttt cttcatctat atgctttggc
     7921 aatggtaatt ggaccaatat accatgaaaa ctaggatctt gattgtattt ttcaatttct
     7981 gcaagtagtt cgtcttcaga aatcgtctca ggataacgct ctaccaatga atggattccg
     8041 atttcttttg ctacacgttc tttatttctt acatagattt ggctggctgg attttcccct
     8101 accaataaaa cgactaatcc gggatggata ccattatctt tcaatttttg gattttttca
     8161 gcaatctctg cctgcatttt ttcagctaat tcttttccgt taatcaattc tgccatctaa
     8221 ccactcccag atattttttg tgttttcatt ctaccataga attttcccga tttataacag
     8281 gtttatgatg attcatagaa aaaagctagg acttacgccc cagcttctaa ttctttcaat
     8341 acattggata aaacaccgtt gacgaattta cgtgaacggt catcactaaa tgtttttgcc
     8401 aactcgattg cttcatttaa agcaacagcc gctggtacat ctgtcacata agtcatttca
     8461 aaaatcgcga tacgcaagat caccaaatcc atttttgaga tccgattgat gtgccaattg
     8521 ttttttaaat gtttttcaat cactttatcc aattcatctt tttttgcaca gacaccgcca
     8581 actagcgtat caagataaac aggaacaaac tctgattcat cttcgttgat catgtcgcgg
     8641 tggtctaatt cgattgcgtt gaaaatcgca tcttcttttg ttaggtctgc attgaagtct
     8701 aatggaaata aagcctgcaa tgccatttcc cgaatctcgt gtcttgataa ttctttattc
     8761 attctcttcc tcttcgtctt caaaaagttc ttcgattgtt ggttgttcta atttttcagg
     8821 aacaacagct actacatgga tattgacttc agataaatcg atatccgtca tgaaaaggac
     8881 ttgctggctg acccgttcct gcatttccat tgctacttta ggaactgaaa caccatattc
     8941 tagataacag taaagatcta ctttcaagcc atcttcctcg tttgctaagt aaacaccttt
     9001 tccgtgagct gcacgtccta ataattcagt gacattgctg gcaaaagttc cgcgcattcc
     9061 gtatacgcct tctactttag aagctgcaat cccgataatt acttcgatga cttcaggagc
     9121 gatcacgatc tctcctaaat cttggtttgc gtttaaaatc aaattttttt catctgccat
     9181 ttatttattc ctccctttgc ctaagcacga gatacatccg aattctctat gatgcggcat
     9241 agtgatcacg tggtaagaaa actgccgccg atcagctgca aactgctatg aattggagaa
     9301 aacacccaac tttattatct cttacagctt aaaagttctt ctatagtgta tcattaaatc
     9361 gagtgatttt ctataacaaa gtctgtgaac actgattttt acgcttactt cttctattga
     9421 tgaaactata agatgatcaa ttcttttggt gaacgagtca acacacgatt tccatcttct
     9481 gtgatcaaca agtcatcctc aatacgtact ccaccgattc ctggtaagta gatccctggt
     9541 tcatcagtga tcacatttcc tggtacaaat tgcttttctg cacgagaaga aacgtttggt
     9601 ccttcgtgga tctctaaacc gatcccatgt cctgtagaat gtccaaatgc ttccccgtaa
     9661 ccatgttttg aaatataatc tctagcaact gcatctaatt ggacaccagt cattcctggt
     9721 tttgcagcat caataactgc taattgagca tctaagacta tttgataaat ttcttttaat
     9781 ttttccccag gatcaccaat agcgaatgta cgtgtcatat cggaaacgta cccttcgtaa
     9841 tagcaaccaa agtccaatgt gatcaagtca ccttgttcga tgattttttt gcttgctaca
     9901 ccatgcggca tagccgaacg caatccgcta gcaacgatcg tatcaaaaga aacacctgaa
     9961 gcgccaagag aacgcatata aaagtctagt tgatttgcga cttcaatctc agtcatgccc
    10021 ggttggatca ttttcaggat gtgatcatat gccatatccg caatatgaca tgctttttcg
    10081 ataatagcga tttcttcttc atctttcact tctctgagtt cttcgatcat ctgtgaaaca
    10141 ggtactaatt ccgcatcgat gatttcttct aaaaccgaat attctaaaaa tgagactgtt
    10201 gtttcctcaa aagctaatga gtgcagttct tctttgcgta ctaagtttgc tacctcatca
    10261 aaaataggtc cgacattttt aataatttca aacccttgag cctgcgctgc cgcctgctcg
    10321 gtataacgaa aatcagtaat aaaaaatgct ttttccaaag taataacagc taatcctgtc
    10381 gtacctgtaa agttcgttaa gtaacgaaga ttgtacggac tggtaaccaa aaatgaatcc
    10441 aaattttctt cttgcatttt tttgcgcaat ttttctacac gtagcatcat aatgaaactc
    10501 cctctttctt tgcacgcttt tttccattat agcaaacttt tcttcgtaca cgacagttaa
    10561 ataaaaattt ccggaaaact tctttttatt ttaaataaat aaagccaaaa aggagtcaca
    10621 gtctcgctgc aactcctttt agtttactat tttgtaaaaa cttatgatcg ttttccaaag
    10681 aaaaatgaga caactgctac caaaatcact gcgcctaaaa tagatggaat aagtgccata
    10741 cctgcagctt ttggtcccca ttcgccgaaa atcgcttgtc caatacttga accgactagt
    10801 ccagcaatga tattcgttac acagcccatc gaatttcctt tactggtgat cgcacctgca
    10861 attgctccga tcacggctcc aacaatcaat gtccataacc aactcataat ataacctcct
    10921 tctttgctaa tcaattttag catatgggat ttaaagggga caaataattg gttttgctat
    10981 ttcatatttt cttctgcaaa gtaaaaaaat aaagaaaacc tcctcttaga agttttcttt
    11041 attttttact tgcgtttaaa catactacga ataccacgtt tcactacatc aaagaaactc
    11101 aatgattgta ccattctatc ttcatctaca tattctctga tcgcacgaag cactttttta
    11161 ggttctttgg aagaaaatgt gtatgtacca ttttttttcg tttgaatggc atatcgagga
    11221 atccattttc cctttagcat gactgatgct agtacataat ccacttcttc ccatggaatt
    11281 tggatatatt tgcgtctatc tcgcgcatgg taaaactcaa aaccattatt acctatcatg
    11341 atttgcccgt aatctgtcag acccgtaaac gaagtagcat caattaccaa gtctactttt
    11401 gtattgattg attgaaccat tttctccctc cagatatttg tttatgaaca gtatactata
    11461 taaaaaaagc tcgggacaac gcccgagcta tatttttatg gattataaaa gtccgatgac
    11521 gtgtccaaca acaccgacta cgaataaacc aaggatgata acgattggag aaaccttttt
    11581 cttaagcaac cacatacata acaatgttaa tcctaatgca gctaaaccag gaatcaattg
    11641 gtctaagttg tcttgtaatg ttgttacctt ttcaggagaa agggctaagc cattattaac
    11701 ttgttcaaat gcactcttga ttccttcacc acctgaaggc aatttatccc attcgatgta
    11761 agcaccttta tctaatttta cagttgatac aactggtaag aatttgattg atacccatct
    11821 ttgtacaagg gcagctaata cgaacatccc taaaattgaa gcaccttttg ttacgtcttg
    11881 taacaatccg ccagaaaggt catcagtaat tttagaacca gctttgtagc caaattcttg
    11941 tgtataccac atgaatgccc aacgaataat gttccaacct aagaagaaga tgattggtcc
    12001 aaggatattt cctcccatag ctaatgaagc acctagtgca cctagcattg gacgtactgt
    12061 aaaccagaac actgggtcac caacaccagc taaaggaccc atcataccta ctttaacccc
    12121 ttgaatcgct acatcatcaa caggcgcgcc attcgcacgt tcttcttcca gagccaatgt
    12181 aacccctaaa attggagaag caatgtatgg gtgtgtatta aagaattcca agtgacgttt
    12241 taatgctgca gaacgttctt cttttgtttt gtataatttt ttgattgctg gaatcataga
    12301 aaatgcccag ccaccgtttt gcatacgttc gtagttccat gaaccttgaa tgaaggtaga
    12361 gcgccacgca acagctaaac gatctttttt tgataatttg attttttctt ctgccatttt
    12421 attttcctcc ttcaagatta atagtcattc aaaatgtcgc ctaaagggtc accagagttg
    12481 cttccgcctc cattatttga tgaaccaccc attttagaaa ggttcaagta aataagagct
    12541 aatgcaacac ctaaagcacc tagagcaatc aatgttaatt gagaaattgc agctacaacg
    12601 aaaccaataa cgaagaatgg ccatacttct tttgtagcca tcatgttgat taccaaagcg
    12661 taacctacag ctacgaccat tcccccaccg atagccatac catctgttaa ccatgctggc
    12721 attgattcaa ggaatgattg cacagtttgt gctgggataa atagcaacgc tgctgctggg
    12781 accgcgatac gaaccccttg taaacataca gcaatgatgt gccacatttc aaccttacgg
    12841 atgtttcctt cttcagcagc cgcatccatc aaatgaacga ttggaacagc gattgtacgt
    12901 acgatcattg ttaagaacaa ccctgcaaca gccaaaggaa cggcaatcgc gatcgcagaa
    12961 ggaacacctt taacaccttg tccacctaaa actaaaataa ttgctgatgc tactgatgct
    13021 aatgcagcat ctggtgctac agcggcaccg atatttgccc aacctaaagc gatcatctga
    13081 agcgtaccgc caagtacgat accagcttcg aggttaccgg taactaaacc gatcaatgta
    13141 catgccacta gtggttgatg aaattgaaat tcatcaagaa taccttccat accagctaga
    13201 aaggcaacta atacgactaa aattattgaa ataatagaca tgataagcct cctataaatt
    13261 ttaatattat ttattacgct tttgctaatt cagcttttgc ttttttcaaa atatcatcca
    13321 tgttatcgcg tgaatcatta ggtactttac gtacatcgaa tttcacacct ttttgttcaa
    13381 gcttctcaaa cgcttcaacg tcttcttgtc ccatagacaa tactttactt acaacgactt
    13441 taccgaccga atgcgccatt gacccaacat ttaattcttt gatgtctacg ccaccttcaa
    13501 cagctgtcaa tacgtcttca ggattttcaa ataaaagcaa tgctttggta tttccaaaac
    13561 gagggtcttt tgctacttcg atcattttgc taataggaat aacatttgct tttacacctg
    13621 gaggagcagc ttgttcgatt agtctcttac gcagatcatc ctttgatact gcatctgaaa
    13681 caacgataat acggtttggt tgcactgctt ttgtccaagc tgtcgcaact tgtccatgta
    13741 acagacgtga gtcaacacgt gctaatacat atttgatttt tccgtctccg accacagttc
    13801 cttctggaag tgctccttta ggttgtgcgt cttcaactgc tgctttcggt gcttctgccg
    13861 gttctaattc ttccggtttt acttttacac cgtcttttgc tgtttcaatg atgtgtgctg
    13921 caatttcatg agcagattcc attgaaaagc gtgatgcgta ggcttcaatc aacattggta
    13981 aattcaaacc gctgacgatt gcccatttat ctttgtgctc ttcgaataat gtgtttgatt
    14041 gattgaacgg tgttccgccc cataaatcaa ctaagaataa cacttcgtct tgattatcaa
    14101 aagaagcaac cgcttcttcc aatttggctc tcaagtcatc tggaccttca cttggtttca
    14161 aaatcacggc tttgacgttt tcttgttcgc caaagatcat tgaaccggat tgtaagattc
    14221 cttcagcaaa ttgtccatga cttgctagga taatccctac catatttata cctcctattt
    14281 acttttgttg taacaaattc aaaaatatta ataattgcta accattacaa cagaattatt
    14341 tgaccttttc caacaaccat tcttgctaca cctttttgat tttcgctagt agttccatca
    14401 aaaggatttt cctgtcagca ggtacttttc gaatctctaa ctccacttct tgactgttca
    14461 agtaacgaaa ggaatcaata tcttgttgat ttactgaaac aaaattggta atcattgatt
    14521 tgccttcagt aaaactcata ccgccgatat tcaacgactt caactgtaca ccgcagcgga
    14581 caaccgcttc aacatcttgg ggattggtga ataaaagcat cacttttgct cgtgataata
    14641 atccactatg atagatttga atcattttct gcacggttat tacatttgct gtgatccccg
    14701 gcggtgcagc ttcttttagt agaaactttc tcaatttatc ttgagcgact acatcgctga
    14761 tcacaatgat acgttcaaca cctgtttgct tcgaccaaac tgttgcgact tgcccgtgga
    14821 ttaaacgatc atctatacgt gccaatcgaa tatccatctg caaaactcct tcttcctcac
    14881 tacttcacag aaacccctat catttctgtg tgaacaacca cctcctagca cacactacta
    14941 taagcaaaaa gcgtgccaac ttttttacga tacactaatt gaatttaaag gattcttaac
    15001 caatacacaa aaacgataca ctataaaagt gtatcgtttt tgtgtattca tggcacagtg
    15061 tattcttttt tatctaactg attatggact agacgtagca gataatacat ttcttctatc
    15121 ggcactttta cttttatctg atcttccaac agaacaactg aggctgccaa ttggctataa
    15181 agaggatcat tcgttgtatt tgtcaattcg ttttcttcca cagtcaatgg ttcattccga
    15241 ataatacgtt cgatcattcc tgctgtgtgc atcactaggt tgattttgaa tccatattct
    15301 tcatcgccga ttcccgtcgt ctgagagatt tgtgtagaaa attgccacat tggatcgatt
    15361 aacttactac cattaataaa gatgaagtta tcagagataa actcaacaca cgtattttta
    15421 gctgactctt catctaggga gatgaattca gtttcctcca attctgattc catcagcaac
    15481 tgatccaaaa tgacttcgat attttgatca ataaaacgtt ctaaaggaat aaatggggct
    15541 tggatattca aatcagtaat cccagtagta gctatgatct gatattcttt ttggataact
    15601 ggcagttctt ctttcacttc cacaatcgac aaagctagaa cttctaattc tgtttcttga
    15661 cgcttgttta ccgctcgttc gatcaattct ttgattcttt gtgctgtacc ttcgccagat
    15721 gcgcagatag ccaaaacagc ttttttctta tgaataggtt ttgcttcctg aacagattct
    15781 acagaagata tttctgcgta acctcgaaaa tttttcaatg attcatgcaa catatctaga
    15841 tctgtcccta atactgagac ctttcgtaca gtttccaaaa ctagagaagt tgttaccata
    15901 tcaacagtac gtactggaat tccagtgtct ctttgaattt gcgaattaaa cgacgcaagg
    15961 gaccccatat ctactaagag tatgaccccg cttccttcat ctacttcttg tacattgcgt
    16021 tcaattcggt ccaatgctgt tttcggatcc atatccaaag gcatgtctac tgctcgtacg
    16081 ttctctgcat ctaacaactg ttggacaact tggaccatac tgcttgctgt gctgtttccg
    16141 tgagctgcaa cgactacacc gattctgccg cttgcttgat ttgcccgtaa cgagacgagt
    16201 agaacggtca agtaatagtc ttcactttct ggaatctcca cttgaaaata cgtttcgata
    16261 taactacgca cttctttcgc aacagcatat tcatctggaa aatcaaaagc catctcacgg
    16321 atgttatcat tcgtatgccg ttcttctcct aagttgattt ttttcaaaaa agaactgata
    16381 tgcaagctca tagcataaat aaagttttgc tgaaactcat atggtaaaga tcttttgacc
    16441 attacataaa tctgattggt cacttctatg attttcggat ctacaaattc tgctaatttg
    16501 ctgtctgcat taaaagaaaa accatggtct ttatagaaag acttcagatg gatgttgata
    16561 tctgtagaaa tgaaatgatt gattgcttct tgatccaaac catccgactt cagtaaagct
    16621 gctttatctc cgatgatatc atatagatta taaggtaact cataggagtc cgattggatt
    16681 ttcatgatcg tatcatttgg tgctacagtt atctttggct ccaatatttt agaaagctct
    16741 gccattgcac tgcggttgct tgctaattga atcaatccgc tacgaatccc ttctgtcaaa
    16801 tcatcgattg tgatcgagat ttcgggtgat tgcatatgat ttagaaatcc tcgtgcacat
    16861 actaactgga tatttgactt caattgtcct atatttccat aggtcacact gccgatcaat
    16921 gcttttgcta catcttctgt caaagaaatc tttcgctgcg tgcggtttgc ttcgtgggca
    16981 atcatgattt ttactaaatc gactttctca tttgcaggac gtttttcaaa agccggaagt
    17041 ttgatattga ttgggatacg ccgaacaaaa gtttctaata gagaagaacc tgggtcttct
    17101 gtggtcgcac ctacgatccg tacatttgct tcatgggatt tcgttgtttc cccaagacga
    17161 ctgtaagttc catgatccat aaagtaaaag atcatttctt gaccttcagg cggcaaccgg
    17221 tgaatctcat ccaaaaacag cataccgcca tccgcttgat cgataatccc cattttttct
    17281 tcatctgcgc ctgtaaaagc accttttgcg tacccaaata aatgactcat caaaagctca
    17341 gggttattgg cataatcagc acaattgaaa acaatcagtt ccgtttcctc atctattaca
    17401 tgatgcgctt ttgcaaaatg atacatcgcg tgtgcaaaat aagtctttcc tgatccggta
    17461 ggtcctgtaa tcaaacaatt taatcctctt ggcggataca aaatcgcagc ttttgcctgt
    17521 tccactgaat ttttcatgct gccgttagct ccaatgattt ttgcaaagat atccttagta
    17581 tctatttgga gtgtattttt ttcttcagag accggcgttt ctttgtaact gactacatgt
    17641 tttgtaactg gctttacgtg cgtaacgagt gtttgatata tgtaacgtac tggtcgtccg
    17701 tctgtcttta tcagttttcc ttcgcgcact agctgattca gatctttact gctatttgtt
    17761 cgctgaatcc ctaacacttc agcaatttcc tgtgttgtta ctccttgatc atattctgcc
    17821 tttgttaaat gagcggtttt ttctttgaca tacatataaa ttcggtcaat gcgttttgcc
    17881 aatggtatcg ctccttttct atagactatt gtacttaggc ttcaaaacat aatactatgt
    17941 atttatttct gtttcgtcaa ctatttacct gattttgttt tgttgataac acgcggatag
    18001 atagaaatgt tcaatttaaa agaacgaaaa atcccgatac gatttttata atggaaacaa
    18061 gaaaaagaac actgtttacc atataattat ttcactgata gcaaacaata tagcttccat
    18121 caattgaaaa cgatagttat caaaacatcc tgattccaaa ttggcaaaaa aatattaagt
    18181 gtaaaaagac aaagtaaact tcgtagcctc cactacaact tttattttta taaacaaaaa
    18241 aggctgtgac aaaagtcttg tcacaacctc tttaatcata agattcaatt aagcttcttg
    18301 agctactgga taaacagaca cttgtttttt gttacggcct ttacgttcga aacgtactac
    18361 gccatcaact tttgcaaata aagtgtcgtc tccaccgata cccacgttta cacctggata
    18421 gatttttgta ccgcgttgac ggtaaagaat tgatccacct gtaactgttt gtccatcagc
    18481 acgtttagca cctagacgtt ttgattctga gtcacggccg ttagatgtag aaccgccacc
    18541 ttttttgtgg gcaaataatt gtagattcat agataacaac atggtctgca cctcctattt
    18601 atcattaatt gttttaattt ggatatattc tgagttttca gcttctatcg attgtaatcc
    18661 taataaaaga ttttctaata gaatctcagc aatattgttt tgttcttgtg tcattcctga
    18721 agtgacttcg gtataaagat aaccgccttc ttcttcattc atttcaacaa tcggctcaaa
    18781 accggctaat gaggcaatcc cgttgacagt gctgatcgct aacgcagaaa cacccgcaca
    18841 aacgatatca cttccataag gaccagcatc agcatgcccg gtcaaagtga atgaaacgat
    18901 ctgacctgcg tcatttcgct taaatgttcc tttgatcata tataggcctt ctttctcaaa
    18961 cgctattaag cgttgatcgc gtcgataacc acttttgtat atggttgacg atgaccttgt
    19021 ttacggtgtg aatgtttttt aggtttgtat ttgaaagtaa cgactttctt ttgtttgccg
    19081 tgtttttcaa cagtaccttc aacagttgca cctgcaacag ttggagcgcc tacttttgta
    19141 gattctccgc ctactaagat aacttcgtca aaaacaactt tttctcctgc ttcaacgtca
    19201 agtttttcaa catagattgc ttgtcccact tcaactttta cttgtttacc accagtttta
    19261 ataattgcgt acatgctata aagcacctcc ttctgatata cttagactcg ccatccgaag
    19321 tgacagcata tgctgtgctt attacttgtt ctgagcggtt gtagctgtgg agtccacaat
    19381 tacaacagta ctagcttacc agaacagcaa ccgttagtca actaaattta aaatcaaaaa
    19441 gtaaaacaaa aattcaagca atacttctgc ttttctatcc tcacagttct accaaataaa
    19501 atagtcgcat tgtgtttctt ttattcttat aataaaagaa aaaggaggag cataaatgga
    19561 atacccaaaa gaactgatac aagaagcttc tttaagaatg aacggccgtc ctgtagaagg
    19621 atttatcgct gggcaaggac ctcttcatcc taaactgatg ctcgtgggag aagccccagg
    19681 gaaaacagag attgatacgc atgttccttt tagcggtcaa gctggaaaag aattgatgca
    19741 ggcactttct tcaactggct taacacgaga agaggtgtat atcaccagtg ctgtaaggag
    19801 ccgcccgtat cgcgtgactc atcggatcaa taaacggaca caacagtcag aaactgttta
    19861 tccaaatcgg acacctactc gttccgaagt atttgcgcat gcaccaatat tggattacga
    19921 actgatgaag gtccagccaa aattaattgc gacacttgga aacattggtc ttcaacgttt
    19981 acttggcaaa gaatatacgg ttaccaaaaa tcatggtcaa ctctatacag ggcctgtctt
    20041 acaattaaca gaacaaaagg atgcctacga atggtcagaa aaaaactatc gaattcttcc
    20101 actttttcat ccagcagcta ttttctacaa tcgctccctt gtacctgtta tccatcaaga
    20161 ctggcaagta ctagcttcac ttttacagca atattaaaaa gaacagacaa gaccataaaa
    20221 tcttcagttt acagtcttgt ctgttctttt tcattgtttt aatggctaat tgcagtatat
    20281 ttcgcatgta aagctgtcaa cgcatgagca actgtttgat gttgatgcgt gaatgtatgt
    20341 tcccacaagt gagaaagttg tttttctact tctggctgca aggaagtata gataatcgtt
    20401 tgtcctcttt gctctgcttt ttcttgaaat tcatcaaaaa ctgcacgtgc cgtcaaatca
    20461 atacttggta cgcctcgcat ggagaaaatg acaccatcgt gatgatttag ttcttccaat
    20521 ttatttttga gtttttctga agacataaaa aacagtggac cactaatata cactactgtc
    20581 cagttattca gcttttccgt ttctggcagc cccattcttt gccaatcgat cgtttctaat
    20641 gaaatcgtga tagcagcgct tttgacaaca aaaaatacac agccgccgat caccccgatc
    20701 acgatagcaa tacttaaatc aaagataact gtacaaatca tcgtaaggat aaacaataga
    20761 actgctgacc aatatttatt agtaaatagc tccttgatcg tttcccattc attcatacgc
    20821 caagccgtca caatcaaaac acctgcaagt gctggcattg gaatctgtga catcactggc
    20881 gcaaaaacaa gcatggagag cagtaagaca actgcatgta tcataccagc taatctagtt
    20941 tgtgcaccag atttgatggc cacgcttgta cgggcgatgg ccgcagtagc aggaatcccg
    21001 ccaaagaaag gaagcaacag atttccaatt ccttgcgcca ttaattcttg attgctatct
    21061 aagggcttat ttgccattcg gccagccgaa gcaccacata gcaagctttc aatcattcct
    21121 aataaagcaa tgctaatagc tggaactgcc acttgttgca aggcagaaaa gctaaaatct
    21181 cccatattca aacgattact actgatcaat gtttgtggaa tcttacccac agttgctact
    21241 ggtaaatgga acaatagcat aactgctgtt gccaaaatga tagccagcag cgaactaggc
    21301 attcgtttcg cccatttttt tggatagata aacatcccta aaatgactag ccctcccatg
    21361 atcaaagagg ctgtgtttat cggaaatcct atcgtttgat aactacttag tttttcaata
    21421 acgtttgtac cttccgagtg aacaccaaat aaattatcga tctgacctaa tgcaataatg
    21481 atagcaatac ctgaagtaaa ccccgtaatt acgggtgctg ggataaaaga agtcagtgtt
    21541 cctaatcgga aaagtcctgc tgcaagtaaa aatagacccg ctaaaaaagt agcaagcaat
    21601 acaccttgca ttccctttgt tgccgcaata gacatcaaga tagctgccat cgcacctgtc
    21661 ggacctgata tttgataaaa accgcctgat aaaccgccaa taacgatacc ggcaatgata
    21721 gccgtaatca aacctgcggc cgcatcagct ccgcttgata ccccaaaagc caaggctaaa
    21781 ggcaatgcta cagcagctac tgtgatccca gccaaaagat ctttctgaaa tcttgaaaga
    21841 tcataacctg aaaattcttt ttttagtgag gaaacataat ttttgaacac ctatctaact
    21901 cctttttcat gaaattttct aaaataccat tcattagaat atcatatttc atgagataaa
    21961 gtaatatttt tcacaaagtt tcctgttaga taaattctta ttttttctat atttataaga
    22021 aaatagaaag caatgccaac agagctagcc caccttgttt gagtaaaatc caacgatcgc
    22081 tagtcaaact gccatataat gcaacaaaaa aaatagatat taatagcata cttgtgattt
    22141 ctttagatgc ggaagaaaga taagctccat aaagtagtcc cactcctaat agtccattgt
    22201 acactccttg gttcttaaac aatgtttgaa cagattttct gcttaactct tctttactca
    22261 ttttaaaaac cctacttgtc gtgtcagaat cagttctcac cgtttcgata tacatgatat
    22321 aaaaaaattc aattgcaact agagtcacta aaattctaga tagtatcgtc atatcttctt
    22381 cctcttttct gatgagtttt atttttttgc tccttataat tgtactcgat aaggatgttt
    22441 ttttatttta tttgcctttg aaaacaaaca atattggcac aaaaaacaaa gaaaaaccgt
    22501 tagaaacctc agtttctaac ggttttttag cgtcacagga gggattcgaa cccccgaccg
    22561 tccgcttaga aggcggatgc tctatccagc tgagctacta tgacaagaat cagcggaaca
    22621 tcaataatta tagataatta aattgcttct gtcaatacga aatagttatt taattagcaa
    22681 ataatgaatt attttggccc aactactaaa tcaatctttt aaaacgagac attctccttc
    22741 agctgttcta aggttatcaa ttgaaaacag gagctacatt gacttcatgc tactcctgtt
    22801 tatttagatt tcctgtatcc tttaattgac ttacaaatat agaaactttt acatcaagtt
    22861 cgaattgagc cctcgattca atcgattatc tacaggctgc cataatcatg tatcacaact
    22921 cgattgtttc tgattccttt gtcattaaga tcacaccatc acctaatggc aagattgtcg
    22981 atgtcaaatc gggatgatcc atcacaacac gaagaaattc atttaattta cgatggatag
    23041 ctcgtttgcc acggggaatc tcttcatctg ggagcaagat cgttcctcct tggaagatgt
    23101 catctaccat caaaacgcca ccttttctta acaaacgtaa gcattcaggt aaaaattcaa
    23161 tatattttga ttttgcacta tccataaaaa taaagtcata cggaccagac aactccggca
    23221 agatatctgc tgcttgacct tcaagcaaag tcactttatc tgtcagtcct agacgttcat
    23281 agtttttttt agctttctcg atcatgacat cgaaacgatc aattgtcgta acatgagcgt
    23341 tttctccgat tgtcgtggcc atgacacttg atgaaaaacc gatagctgct ccgatctcca
    23401 agattcgttc aggctgaagc tgtccaagta aaaatttcaa aaatacaact gtttcatggg
    23461 gaataactgg tacttctgct acatgagctt cttcttccag tttgcctagc tcacccggta
    23521 attttttttg ctgggtacgc ataaaatcca atagttcttc tttgactaca ggacgatgca
    23581 tcatttcatt gcgcatacta ctcctcctct tcacacatta tacgaacaac tgactaaatc
    23641 tacaagctgc atgagtgttt tctcgtgttt catgttcaga agagtcgttg gatctctgga
    23701 atacatttat gtttttaaca ttcttacaaa aatttctgtg ttcgtctccg cttctttttc
    23761 aactctacaa aggtattcca tgctgtgatt cctgttaggc aaaaagcaat cgtaaagaat
    23821 aacccctgaa tccatgcaaa tgccgttttt gaaaggacta gtccaatata tggaatctga
    23881 aagatgggac taccgataat atttctcttt tttactactc caggctcaat ataagtcttt
    23941 gtatcagctt ttgtataaaa atcttcgtgg ttgttttcaa cggctattac gcgacgcgtc
    24001 gagatttttt ttcctgaatt ttcataatat gtaataatat ctccgactaa aatctcattt
    24061 gtatttttcg gtcgtacata tatcagattt ccttgtgcat aatatggact catttctttg
    24121 tcctcaataa tataaggagt caaaccaaaa attcggggaa ccaagacgac gagaagaata
    24181 agaattaaaa atataatcaa cgtta
//
LOCUS       DAYYAO010000041        23828 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000041 DAYYAO010000000
VERSION     DAYYAO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.043"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHARI_001534"
     CDS             complement(53..787)
                     /locus_tag="ACHARI_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8442584.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHARI_001535"
     CDS             1114..1524
                     /locus_tag="ACHARI_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8442585.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHARI_001536"
     CDS             1517..2218
                     /locus_tag="ACHARI_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8442586.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHARI_001537"
     CDS             2218..3804
                     /locus_tag="ACHARI_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8442587.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHARI_001538"
     CDS             3823..4044
                     /locus_tag="ACHARI_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8442588.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHARI_001539"
     CDS             4041..4802
                     /locus_tag="ACHARI_001539"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8442589.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHARI_001540"
     CDS             5182..5691
                     /locus_tag="ACHARI_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8442590.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHARI_001541"
     CDS             complement(5766..6305)
                     /locus_tag="ACHARI_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8442591.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHARI_001542"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHARI_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8442592.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHARI_001543"
     CDS             7318..8169
                     /locus_tag="ACHARI_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8442593.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHARI_001544"
     CDS             8197..8832
                     /locus_tag="ACHARI_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8442594.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHARI_001545"
     CDS             8852..9625
                     /locus_tag="ACHARI_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8442595.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHARI_001546"
     CDS             10033..10830
                     /locus_tag="ACHARI_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8442596.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHARI_001547"
     CDS             10808..11221
                     /locus_tag="ACHARI_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8442597.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHARI_001548"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHARI_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8442598.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHARI_001549"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHARI_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8442599.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHARI_001550"
     CDS             15998..16558
                     /locus_tag="ACHARI_001550"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8442600.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHARI_001551"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHARI_001551"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8442601.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHARI_001552"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHARI_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8442602.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHARI_001553"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHARI_001553"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8442603.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHARI_001554"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHARI_001554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8442604.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHARI_001555"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHARI_001555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8442605.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHARI_001556"
     CDS             21905..22483
                     /locus_tag="ACHARI_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8442606.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHARI_001557"
     CDS             22535..23017
                     /locus_tag="ACHARI_001557"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8442607.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHARI_001558"
     CDS             23134..23508
                     /locus_tag="ACHARI_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442608.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23621..>23828)
                     /locus_tag="ACHARI_001559"
     CDS             complement(23621..>23828)
                     /locus_tag="ACHARI_001559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442609.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7782 a         4286 c         5114 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtagactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DAYYAO010000042        23658 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000042 DAYYAO010000000
VERSION     DAYYAO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.044"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..374)
                     /locus_tag="ACHARI_001560"
     CDS             complement(<1..374)
                     /locus_tag="ACHARI_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442610.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGS"
     gene            complement(374..823)
                     /locus_tag="ACHARI_001561"
     CDS             complement(374..823)
                     /locus_tag="ACHARI_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442611.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTMVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(827..1444)
                     /locus_tag="ACHARI_001562"
     CDS             complement(827..1444)
                     /locus_tag="ACHARI_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442612.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(1444..2352)
                     /locus_tag="ACHARI_001563"
     CDS             complement(1444..2352)
                     /locus_tag="ACHARI_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8442613.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(2365..5124)
                     /locus_tag="ACHARI_001564"
     CDS             complement(2365..5124)
                     /locus_tag="ACHARI_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8442614.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMDVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(5121..5825)
                     /locus_tag="ACHARI_001565"
     CDS             complement(5121..5825)
                     /locus_tag="ACHARI_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8442615.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(5837..8146)
                     /locus_tag="ACHARI_001566"
     CDS             complement(5837..8146)
                     /locus_tag="ACHARI_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8442616.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(8341..8805)
                     /locus_tag="ACHARI_001567"
     CDS             complement(8341..8805)
                     /locus_tag="ACHARI_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442617.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(8805..9431)
                     /locus_tag="ACHARI_001568"
     CDS             complement(8805..9431)
                     /locus_tag="ACHARI_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8442618.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(9438..9803)
                     /locus_tag="ACHARI_001569"
     CDS             complement(9438..9803)
                     /locus_tag="ACHARI_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8442619.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(9793..10131)
                     /locus_tag="ACHARI_001570"
     CDS             complement(9793..10131)
                     /locus_tag="ACHARI_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442620.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(10121..10447)
                     /locus_tag="ACHARI_001571"
     CDS             complement(10121..10447)
                     /locus_tag="ACHARI_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8442621.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(10428..10706)
                     /locus_tag="ACHARI_001572"
     CDS             complement(10428..10706)
                     /locus_tag="ACHARI_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8442622.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(10708..12072)
                     /locus_tag="ACHARI_001573"
     CDS             complement(10708..12072)
                     /locus_tag="ACHARI_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8442623.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(12085..12654)
                     /locus_tag="ACHARI_001574"
     CDS             complement(12085..12654)
                     /locus_tag="ACHARI_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8442624.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(12626..13813)
                     /locus_tag="ACHARI_001575"
     CDS             complement(12626..13813)
                     /locus_tag="ACHARI_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8442625.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEETQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(13877..15604)
                     /locus_tag="ACHARI_001576"
     CDS             complement(13877..15604)
                     /locus_tag="ACHARI_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8442626.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(15601..16053)
                     /locus_tag="ACHARI_001577"
     CDS             complement(15601..16053)
                     /locus_tag="ACHARI_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8442627.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(16165..16545)
                     /locus_tag="ACHARI_001578"
     CDS             complement(16165..16545)
                     /locus_tag="ACHARI_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8442628.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(16542..16928)
                     /locus_tag="ACHARI_001579"
     CDS             complement(16542..16928)
                     /locus_tag="ACHARI_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8442629.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(16930..17208)
                     /locus_tag="ACHARI_001580"
     CDS             complement(16930..17208)
                     /locus_tag="ACHARI_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442630.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            17256..17984
                     /locus_tag="ACHARI_001581"
     CDS             17256..17984
                     /locus_tag="ACHARI_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8442631.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(18130..18606)
                     /locus_tag="ACHARI_001582"
     CDS             complement(18130..18606)
                     /locus_tag="ACHARI_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8442632.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(18566..18922)
                     /locus_tag="ACHARI_001583"
     CDS             complement(18566..18922)
                     /locus_tag="ACHARI_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442633.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(18900..19085)
                     /locus_tag="ACHARI_001584"
     CDS             complement(18900..19085)
                     /locus_tag="ACHARI_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442634.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(19086..19358)
                     /locus_tag="ACHARI_001585"
     CDS             complement(19086..19358)
                     /locus_tag="ACHARI_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8442635.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(19355..19555)
                     /locus_tag="ACHARI_001586"
     CDS             complement(19355..19555)
                     /locus_tag="ACHARI_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442636.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            19683..19895
                     /locus_tag="ACHARI_001587"
     CDS             19683..19895
                     /locus_tag="ACHARI_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442637.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(19928..20122)
                     /locus_tag="ACHARI_001588"
     CDS             complement(19928..20122)
                     /locus_tag="ACHARI_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442638.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(20135..20317)
                     /locus_tag="ACHARI_001589"
     CDS             complement(20135..20317)
                     /locus_tag="ACHARI_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442639.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(20314..20454)
                     /locus_tag="ACHARI_001590"
     CDS             complement(20314..20454)
                     /locus_tag="ACHARI_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442640.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(20944..21309)
                     /locus_tag="ACHARI_001591"
     CDS             complement(20944..21309)
                     /locus_tag="ACHARI_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442641.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(21349..21651)
                     /locus_tag="ACHARI_001592"
     CDS             complement(21349..21651)
                     /locus_tag="ACHARI_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8442642.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(21651..21950)
                     /locus_tag="ACHARI_001593"
     CDS             complement(21651..21950)
                     /locus_tag="ACHARI_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442643.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(21943..22107)
                     /locus_tag="ACHARI_001594"
     CDS             complement(21943..22107)
                     /locus_tag="ACHARI_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442644.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(22113..22382)
                     /locus_tag="ACHARI_001595"
     CDS             complement(22113..22382)
                     /locus_tag="ACHARI_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442645.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(22394..23143)
                     /locus_tag="ACHARI_001596"
     CDS             complement(22394..23143)
                     /locus_tag="ACHARI_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8442646.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(23140..>23658)
                     /locus_tag="ACHARI_001597"
     CDS             complement(23140..>23658)
                     /locus_tag="ACHARI_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8442647.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
BASE COUNT         6577 a         5051 c         3646 g         8384 t
ORIGIN      
        1 gacccaacca gttgttcaaa actaattggt gtccgatagc cttctggaac agttaaaata
       61 accgaatcat tcccgccact actttttccg tttaaagcca caaaatatat agaaactgtt
      121 tttccttcac gataaagctt tgctgtccct gtattaccgt tagtgacaac cagatttacc
      181 acttcatatc tgttatcatc agtcgtcaaa acacgtttat tattgattgt tacagaacct
      241 gaaaaattct tatgtcctgc aatggcttgg tctccatctg tctgcaccaa tgcttcttta
      301 ccatcaatag cttctacatg cgtttttaag tatttagcaa ctccgtcttc ttttaattga
      361 acaatatcag ccattaaacc gtccccactt tctcaaatgt aatattcgct aatccatcaa
      421 gtttcacttt atcggttgct gacattaaac ctgctgtcgt agttgtagca ttacctggat
      481 ttttctgtgc tccagctgca attccatcca acttagtttt atctgtggac gacatcaacc
      541 catttgctgt agttgtagct acagctgtag ttgtggcatt tattccagga tcacctttat
      601 ctccctttgg taaaacaaaa ttaaatctag ctgcagatga tgttcctaca ttcgtaacag
      661 aagcggttga accactagaa acggttccta tggtaattgt tgctgcttgg ccaggatcgc
      721 ctttatctcc cttcaccatt gttggtttgc cttctatagc attccaatga gtttgtggat
      781 aaacctgtac attgctttgt tttattttta cgatatctgt cattctctat acctccccaa
      841 ttttttcaaa agtaaaattt ggaattcttt cgtttgtgta attttctgct tgatttacag
      901 cttcttggaa tttttgatct acatatgact gattaacacc accagtccca ctaccacctg
      961 tagaactaat tgttccatct tctgcaattg atatatttgc acctgctttt aatattttta
     1021 gagattctaa ttttcctttt aattcttcag agaaattgaa gtctgtttgc ttagttgcag
     1081 ataaaacacc ttcttcagta acttctaaga gttccccaac ttttatacct cctaattgtt
     1141 caggagtagc aatcggcaaa atatatgttc caccttctcc atttacgatc ttttgaaaca
     1201 tttcagcagt aagaatacca tcgcttgtct cgcttgcata aggtagttca gtaattgcat
     1261 tctctaatcc taaatcagct ttcgttaaaa tgactgcacc atatttacca ttaaccgaaa
     1321 gaacttttga ttgtcctgat atcatttttt ctaagcctaa aactgcagag acatgtgtaa
     1381 ttggcataaa ttgacgttta acaccagatt catcagtttc catcattctc tttactttaa
     1441 ccatcaaatc accccaactt tctcaagagt gaaaacattc tgttttggat catcaacagt
     1501 tgcaataatc aaagctcctt cttcaattgg aaattctact gtttcaattt tttcgacttc
     1561 gtgattatct gaaaagagat cgtcttgaag aatatcagtc acttcaattt caccgtattc
     1621 aatcgtgaaa agtgctgctt tcattttttg atacaaataa tttagatcgg ataggaaacg
     1681 ttcagaaatc gattcatggc gtacaccttg aagatctaca cgtgcatcca ttaattcggc
     1741 taacattact ccacctggat cgatagactt caaaatatcc ttgattgatt caaaccatga
     1801 caagtaatct gattcttgtc cttctctcca atcttggaaa ctattctcct gttcctctct
     1861 ccagcgatca aattcttctt tcctttcatt catccagtcc gtaaaatcgc ctttattttc
     1921 attaataaaa tctgtcatat cagcaattaa atcttcaatt gattgccaat aagaacccat
     1981 ttctccttct gtttttgaaa cagcattgat gacaaaataa gagaagtctt gagttgttcc
     2041 aatcaagtct tctcctttaa atataatgaa attggctgtt tgtcgatgca aacactgcat
     2101 ggaatattta tcaaaaatat atttgatttt ccctttttta gcatccacaa tttttgtttc
     2161 taattggact ggatattttc cgccaacaac tgattcaaaa tataccttac attctgataa
     2221 atcatatgga agaccatttt cgacaattgt agcttccata acttcagtgt ttttattgcc
     2281 ttgtcgaact tgaatcatcc ccacgtaatt atagggttct gttgtactta atatgacatt
     2341 ccactttgcc attaaatcac ctccctattt tggtggtatg acaatggaag aaattgcact
     2401 tgcactataa tactgtcgat ctaactttcc acaaatcata cctaactctg tgttttgttc
     2461 ataagtttgc atacgaccat tagctaagcc tcggataacg ccagtatgtc cataagttcc
     2521 atctgcaaac catgaaccca cttgtccacc tcttgcccaa ttaataattg caccaactac
     2581 taattgatca tatctagggt tttgaatcac tttccaacca acagcagacc aatcataggc
     2641 aataccaata tcagatgctg cagaagtatt tcctatcaca tgagttaaac catattttgt
     2701 tccagcaccc atgccacaac cacccagata tcctgaatat tctgcagaca agccataaca
     2761 ctgaccattg ccaattcgtt gtccaatcaa agactctaaa tgttttagtc ctgcttctcc
     2821 agtagctcct cctggtttta aatctttaaa tttgttatac cagttaactg cataatcttg
     2881 acgttcagga tgtgtcgctg ccggacgttc atagtttcgt tcaaaagcat atgctgcttg
     2941 tcttggatca gtacaggcct taaatccatc aaccgtcgta ggttgtacta cgtccatcca
     3001 ttgtccattt gtaaatgtcc aaataagcaa tcgaacttgt gcatctaagc tcgttattgg
     3061 ttctttaata cctgcagcat taaataagtt ttgaacataa acttttccat cccatgttgc
     3121 tggaccaaca agaggatatg aagaaccatc ccactgaact aatccgtatg ctggtccacc
     3181 tatttgaaca gtatcaggat caaaagttcc tcctgtttct tgttgaatgt tccccaatat
     3241 tccacatgca gattgtttcg taaatccgtt attacacaaa atatcgtata ttctccaagc
     3301 tctcttttct gcatctgttt ttaattcatc aggataccca ccaggtgttt ctcctccgcc
     3361 agatggaccg ccattttgtc ctggtatcac ttctttacct ctaacagtaa gttttccctg
     3421 aatatctaaa tcaccgaaat aatatgcttt tccgtttcct aataacacaa atcctttttc
     3481 tgcagttgga gaaattaaaa tgtattttcc atcgccattt gtacgtatta ccaaagaatt
     3541 ttcctcaaga ggtgtaggtg ttacagcatt tggaaatgga tttcctgctg aatctgttgt
     3601 tccgatcgtt ccaatggatt cttttctatt ccagaattcc ataccttttt tggttagctc
     3661 catgattttt ttgttttcat tccaaatttg aagcgtgcct tttacaagtt ttaacacatc
     3721 accataagca ttaaaggaag tttcgaatac ttctgcattt attgttccta ctttgatgaa
     3781 gtcagcaaca atttctcctt ttgatgtgat agcaataccg aacgggccat ttactccgtt
     3841 gtctgaataa cctaagccat ttaaattcca acgccacact cgctttgcat tcgccacatt
     3901 tggagtatcc atgataagaa tctctgatgg agctttttct ggacgaaaaa cgacatgccc
     3961 accagaatta ccagtgatcc atgccgtcgc attcaacaca ttttgaacta acgtttctgt
     4021 tcgattgtcg attttctttt tcaattcttg tgcttgagta tttactgccg aagtgtacag
     4081 tgataaatca ttacctaaaa caatatcctt atatttccca agcgttggga aataagtgta
     4141 ttcgaccata cgctctttta tctcgatatc tagctctttc gccctaacat gagcgacatc
     4201 accaaagtgt aaagacgcta gctcttgata catgtcaccg tattcgagtg tgtgttccaa
     4261 cgccaccata ctgactgtat gagtagcttt tggctcatgg atacgatcat tgtcaaataa
     4321 ggttttgccc catttgatca attcatcaac tgttttacaa tctccgtttt cacgtttggc
     4381 aatacgtcga ttctcgttgg ttactccatc aatttctaaa taaccatatt cgataggttc
     4441 tttgtcttca tcataatcgt tatcaggaac accgccaacc aaatacaaac tattgacaat
     4501 tgattcttca tcaatttctt cttcgattgc ttctaaattg atgccaaaat caatccgaaa
     4561 accattatct gatccaatct gttttaccag tttcaaatca tagttatcca tatcaagttc
     4621 cgcgctagtc acgccagtca aattttgatt accgttattt gaaccaatga ttgcttcaac
     4681 tggcgcgact tgtcttgctg taaattggtg cgttgtccct acattcgaaa gatagttaaa
     4741 tttttgatca aatgccaaac tgtttttaag attggtcatg atctgagacc cattgccgtt
     4801 atccgtaaac gacttgacaa taaagttctt attggccata aaaccaatat gccgagctgt
     4861 tacagaaacg gaggtcaaat tctttttgac attataaatt tcaaaatatt gccatgatcc
     4921 atctggtacc ttcgctttta taaaatttcc tttttttaaa taagagcgat actgtccgct
     4981 tcttgaatag ttaccataaa aacgatattg gccattcagc acacgattga tttctggcaa
     5041 atcttcccaa tcaactaacg acgctccgtt cacgctcaaa tcatctggca tttttttata
     5101 agcataaata aattcttgcg tcacagatac acactcctat tccaaaaccg cacttcttta
     5161 aattttccgg atatttttac ctgattccat tcaggctgca atactggcca atcaccacgc
     5221 gtaaataaat tcaaaccttc ttgtattgct tttccaagct gagtatcaac cacgattgtt
     5281 gcagctaacg tattaagtat tgttaggctt ttatcaccga cagcgatcgt tatatctcct
     5341 ccgttcgatt ctatttccag atagggatgt gcaatttcgt caccatgatc aaaaatatcc
     5401 ataacactag aatgaaaaat tttaggtgct tcaccgattt ttcttttgag tggctgacaa
     5461 cgaaaagtaa catcgaatgt ataaaagaag ccccactcgt tttcgaaagg gacttcttta
     5521 tccatgctgc agatagcctc tagatacttg tctggatcat tatgtgtgat taattggctt
     5581 ttaccagtga gccatcgttt gacttctctc agtttcgaat gtggaatagt gatcccttct
     5641 atttccaaat caaaaggttc atagtcatca aacgtctccg tcaattctcc gcttcggccc
     5701 tgaatcgtgt aagtttcgta tcgcttgttc ggcataatat ctggaagctc tgcctcaatg
     5761 atgcaattca tatcaattac tgcattccga tttttccaaa taaaatttgg ttcatctgga
     5821 ttcataaatt gtcgactcaa ataggaacac ctcccaaatc acgaatagcc tgtttgtttg
     5881 ctttagcaaa tttacgattc atacgatcta actcagatgg attgtttgca tcgactttgc
     5941 caatgtgtat atgctgttca atgctacctc cagaaactct tccactgatc cctttgcgtt
     6001 tttcttcgtc tgataatgga gtaactgtgg ttttaccatt cttggcagtc agtaactcag
     6061 gtccagcttc accaacaatc gcttgtccat tgattagatg accgccttgt gctaaataag
     6121 gcagttttga aatagaaaat gattttccac ctacaccagg aacccatttt ggtatcttta
     6181 tattattcaa tccaccaata aatccattga tcaatccaat catggcatta ataggagctt
     6241 tccctacagc aacaatacca tcgaaaatac ctccaaaaat atcaactaga ccttgccatg
     6301 cttttgacca attccctgta aatactccag tgataaaatc tagaaaacca ctgaaaattc
     6361 gttttccagc attataaaaa ttattgaaat ttgctataac accatcgaaa aatccaccaa
     6421 aataaccact taaaaaatta aatatttcaa cagctacatc tgaaacacct ttgaagaaag
     6481 aattcactcc atcatgaaac catttaacgt tattatatgc ccaaattagt ccagtaacta
     6541 atgcgccaat agctataact gctaatgcaa accagcctcc cgacattcca aataaaccag
     6601 caagtccttg ccaaactcct ataaaacttt taacttcccc aacgattttt gtaacagatc
     6661 ccataagtga accaagaacg attagtactg gtccaactgc tgccgctata ccagcaattg
     6721 ttatgatcca attctttgta tcttgatcta gagaccccca ccatttagag aattcctgta
     6781 ataaggaagt tgctttttca aaaaaaggca aaaggcttac ttggactgct tcacctacgt
     6841 cagccattgc taattttgca ttgttcatag cttgatcggc ttgatcaatt gggtcgagag
     6901 tagcatcgaa tgtgtctcct acagctcccc cactttctcc agcagttttt gctaaatctt
     6961 ccaggttcaa agtacctcta cgaattgcat cggccattct aggaccgcct ttagtcccga
     7021 atacttctgc tgctgcattg atggcttctg tttcagaacc tgcatttttt accttgtctt
     7081 gtagttcttc tagtccttga cttaaagatt ttccgtcttt cgcataagcc actgttgctt
     7141 tagataaact acttaaagct gcgcttgaat caacaccaga ttgctcaaat tgtccaagta
     7201 aagtgacccc ttcaccaaag ctcaacccta attgtttaat ttgtggtgcg ccatcaattg
     7261 ctttttgcat caagtcgtct acagattgac cagtattctg cgatgttttc gttgttacat
     7321 caaggacaga attcaaatca tcatattcta gtccataagc atcaatagct tgtcgagcag
     7381 atattgctga ttgcgaaaca tctgtatcat tgatttcagc gtactgtagg agataattgg
     7441 ttgaatcttc cagtttttta tccatgaatc caaattgtgt atttacctca ccaatagctt
     7501 ccccaaccgt ttgtaaaggt aaatgagtat ttgaaccaac gtttttgaaa gactgtgaaa
     7561 gtctatcagc ttgatcacct gttgctccgg tttttgtaat gatggtatca agtgcctcgt
     7621 caacttcacc aaatgctgca agtcctgctg ctcctgccgc tacaataggc gcggttactc
     7681 caacagacat tttttcacca acgcctttta ctttctcacc tgtttcttcg attttctgga
     7741 gtttttttgc tgtatcaaca gaaacgtcac cttgttcttt aagtgcatcg ttcgtctgtt
     7801 ctaatgctgt acgaagttta ttttctccgg tttcagaatt gagtaattgt ttataaagtt
     7861 tttctgattc agcagaatat tcaccggttt ctttgacaga tttttcatat tcctctctca
     7921 gcatttgaga tcgttgttca gccaatccta attgtttttc taatttttgt ttagccgctc
     7981 gcaatttttc tgtttgagtt gcatccttgt ccatcgcaga cacttggttt ttgtactcgg
     8041 cagcggctaa gttcatttct ttgttgattt ctttgattgt tttcgaataa ctgacttcgc
     8101 cattcgtttt aaaattaaga acgacatcag attctttacc agccatttat cttagcgctc
     8161 ctttctacca ccaaggcgat ttatccatag ttacagattg aggtggttca aaatcggtgt
     8221 tttgttgtaa ccactgtaaa taagatttga gccacaagtt aggtgtagac tttaaaaaga
     8281 aactctcact ccaattcaat agagtgagag caacgtaaat ataaaaactc caaggagttc
     8341 ctatctcttc cgattctttt tgtttttctt tctttttgct tgcgcttttt gaaagtcttg
     8401 tggcttcttg gattttttta ggtcttccac ctgaaatgtc tggctagcaa aaatttccat
     8461 acaggtacta taagcagaca acacctctcc actcattccc aaaaatttga aaatagtttc
     8521 tggatcttcc tctaaaccac cagtacgcag catggcataa atcaaggcac gcatgatttt
     8581 tagatcacta ggcgataagt tagcagaaga aatttgtcct tcttttttag atagcatggc
     8641 gttcatatca gattcaaatt gcgaataatc tccaccatac acatctgcta taaattccat
     8701 cgtttgcata gtaaatgaaa tagggaattc tgcgccttga ataattacaa cagcagaatt
     8761 ttttaagtct tcaacgtgaa ttccataatc agataaccga gccattaacc cgcacctcca
     8821 actttagcca atgttttcca ctgttcttca tcgtatacag gttgagcaat gaatttatta
     8881 aataattcta gagaagctcc gtctctattt gaatcaaaac ttgcatacat cacattattg
     8941 tatttcagtc cgatggaaac cagatttgca gttacatcgt caatttttgt ttcttcctct
     9001 gcagtggcat actcttcgtc aatcacattg gatagttgtg tattaggata ccaaactgct
     9061 tttttaccgc cattttcaat atttccaata aatccaaagg cgaagtacgg aaattctcgt
     9121 gcggtatttt tgctaaacgt tactccgctt ttagcaacta atcctttgat ttcatccatc
     9181 acttctatcg gaatacccac gtgatccaac gcaagttcat gttttgtttc tcgcgacaca
     9241 cgacgaaaca ttttgctgga tgcccatttt tctaatgctg ttccattccc tttgacaccg
     9301 atttttgtgg caattggcaa tcgtaaaact tcactataag tcggtgcagt tccaacagaa
     9361 tctggcgttg ccatcatcgc aattaaaatg tcgtccaatc cttcaaaata aaaagtatct
     9421 tgttttccca attaaaatca cccttcccat aaatcaagta tttttctagt cataatttct
     9481 tcaatttttt ctttgttttg ttcatacgta ccacttgcaa aatgttgggc acgttgattt
     9541 gttgtgccat tttcggcaaa gcgccagtaa aaggccgtct cttcaaattg aactttcact
     9601 tgatcgtttt caactactac ttttatttga tcgcgcatat gtttcttttt taagagcgac
     9661 ttaggaatat taggaagcaa ctgctttaga taaaagttag ctgcttcttc taaagattcc
     9721 aacgataatt ttttcggatc aacttttgaa aggtttccta aataatccga tatttcagaa
     9781 aaaccattct tattacttgg cattttccac acacctcaca tatgtgtaat agttggttac
     9841 ggtatcgtcg ttctcatcac cttgtatacc tgtaaaatct gaatatggaa tgccagcatt
     9901 ttgcagcgct tgttcaataa caactaaatc ctgttctgtt ccaagtgtaa aaaaagagac
     9961 ttggtaataa ggtaattttt tatagacttt accggatgcc atttttttgc tgttactcac
    10021 atttgagtac acaatgtatg gatacatcgt ccctaatctg gctttgtctc tgaacactgc
    10081 taactttgtt gatttcagcg ctgttttcaa ttcatcaaag ctaatcgaca taagctaaac
    10141 tcaactccat ttctcttgca tcgggattcg tataaatgcg agtaatgtta tacgttacag
    10201 aatcaatttt gagcgcactt aatttctctg tgatggattt atcccatctg actttaattc
    10261 gtctgacaac gtctgtcttg gcttgctgtg ataaatattt ttcttgagaa gtcacaccga
    10321 gttcttcata gaatattaga cgcttaaatt cgtaaattgt agttggacga tcgtttccgt
    10381 ctgttcctgt tttgatgtct agcaattcgg ctttccatct gagattatta gtctgtctct
    10441 tcggcatttt gaatcgctcc ttgcacgata aatggcgtca tggcattcat agctttgtcg
    10501 agttcatcct ctgaaactct atattcatag gcaatgccgg caaccatcaa aataagatat
    10561 tcttgttggc caccagttgc tgttttgaca taatcttttg ccatatttaa ataaaaagag
    10621 agcaaagaat catccatgcc ctcttcaaaa tgaatatgtg atttgaattt ttcctctaaa
    10681 gacaattctt tagtttgctc ttccatctta accaccaact ggttttgtaa tttcgtagcg
    10741 atatactgcc ggttcaaatg gagaataaac caattgacca tctagcaagt tgtaaatttg
    10801 gaatccaatt tgatttttac cagagaattt ttcaacaagt ttttgaattt ccaaggcacc
    10861 aataacttct tgaattttaa atgcagaaaa atcgccaaaa tataaaactg gcgtgtctgg
    10921 ttcacccttt ttatctgctg catctgtcca atccacagga tagccaacta attggtaacc
    10981 aattccacct tctgcttgtg tgaatggccg caacaaagga aatccatcat ctgttttcat
    11041 tttttcaatg gcagtcaaag cagctcgatt aataataaag cgtccctttt tcatcacttc
    11101 tgtcactggt gtatttttaa attcgattaa tgcatcatat aatttttgcc cagcacctga
    11161 agcagttaga tctaaaggtt ttttaaatgc tacagccttt ttggctaatg caccaggatt
    11221 ttcatttcca gcgtcatcac cattgaacat ataattgatt tctttacgca cataagcttt
    11281 tttcaattct tccacaacaa tatcttcaac tggaacacca gacattttta ataatttttt
    11341 agttactgtt gccaaagcat cgaattcggc aggattaagc aaaatttcat caaactgaat
    11401 agctgtttca gcaatatcag ttgaacgctc tttcttgttt acattcgcat ctgctttctt
    11461 cacaagaatt ggatatttga catctcctga tgttcgcacc actgttccgt atttacgaag
    11521 taaattttct tcttgagcat aagtaataac ttcagatgca attacttctg ggacagtaac
    11581 tgaaccgttg ccagcttcaa tccctaaagc tcgagcttct gcttcagaaa tatttccagc
    11641 tacaaaatta gcaaatgctt ttcgtagttg ttgatctttt tgtttcttgg tcatttttgc
    11701 acgtgcctcc aatccgttct taattgatcc aagtaatcca tctcgttgct gttgagtaat
    11761 cattccagaa cgattttctg aactatcttc actgtctgaa tcttcttctt gatcgttgtc
    11821 ttcaccagaa cggctttcgt ctgaatccgt actattcgat tgatcatctg tattgtcgtc
    11881 tgtttcatca gttccagaat ctgcgcctaa ttcgtcttta attccgttca attcatcaat
    11941 aacactgtca atttcttctt tcacggcttc taaatctgct tcgcgtaatt ctcctgattc
    12001 aactttttca cgtaattcag tcaatcgttg ctcacgacgt gccttcattt ttttcaataa
    12061 ttctttatcc atgtattttt cctcctacgc ttctagcgct tgattaattt ttttgattaa
    12121 tttttttctt tcttcgacgt ttttttctaa ttcatcacga cttcttaatg cagcttctgt
    12181 atcttcgtat gctggcaaag gcacaataga aacctcgtat aattccactt catggattgt
    12241 tcgaagcatt ggttcagaat tgtagtccca cgtttcttca gtcggcacaa aaccaaaact
    12301 acattggttg atgtctcctc gttccataga tttaactaag tcccttgcta ccgttgtgtc
    12361 cggtaaatca acttcgaatt tcaaaccacg ttcatcttct tcaaggcgaa gggttccgct
    12421 tttagtacgt cctagcacat tggaccaatc gtgattgaac aaacaacgaa catccgaatt
    12481 actaattgcc tttgagaatg caccaggaac aatcacttcg cttaagtcat cccataaaag
    12541 tgtttgactg ttgaatacag cagcgtagcc gctaatggtc ctcgtatgag tttcttcatc
    12601 ggaacgtgtt gaaagattgg tgatgtcaat cgtgcgaatt tccttcttct tcatttccat
    12661 cacctccttt caagtcttga tcattcgttg gtaaggaatc atctgttgca tttttctggc
    12721 caatcctaga taagtcattt gaaatataga tagcttgtgt ttctggagta ttctgtttag
    12781 gaaaaccaag catttctgcc acgttatctg gacttgtaat cccggtacga acgatgttgt
    12841 agccaatatt tgtttttgtt gagtaaggaa caaaatccaa aatattaatt ttccattcca
    12901 ctctatagcc agaattaggc ataaaaaaaa gagcggtgta atgttcgctc ttgtttttca
    12961 atattggttt gattgctttg ttgtgcagat acatcatcgc tttttcaata tctgtcttca
    13021 tcagcgattg atacgtatca acattgattc ctagaaattt tcctaagtct tttttgtaaa
    13081 cacctaaata attcaaaatt gccgcgtcat caacaggact ttttaatgtc tcgatggaat
    13141 atccttttcc caaaggaatc atcttaacag aatgatcact ctcattttgc gtttcttcca
    13201 gttgatccaa tatagctttg acaatttttg tttgggcgct attatttgga ttgatgtggg
    13261 cgtctagttt aagcatgaac gcaagtaaac cgcctttagt atatttgtcc gtcaaaactt
    13321 tttcagcgct cagaacgcct tctagagtgt ttcttgcaag atcaattatt ccagcacctt
    13381 ttaatgaatc agttccgatg ttcttaatgt gtcgaatcat ttgaccaggt attttttgac
    13441 cattcatttc aaattcttct tgaagtcgtt cattgatttt agttgttaca ccgtacgcca
    13501 aatgaagctg atccctatcc gttaatggga atgtctcacc attgatcaat aaagtatttg
    13561 tttccaattt ggtaaattcg aatcctgtca aataatcatt gggactcttc aaaatattta
    13621 gtaagtgatg atctttgact tcgttgccgt cgggaccaat aacaattggt gtggccaatg
    13681 ccacctgatt agatatgtct tgaactaatt cataaacatc agaagattcc atgatagatg
    13741 aatccgtcac atatctttga ccataacgtg tgtagtgtcc aaaaatatcc tcgatatatc
    13801 cacgtttttc cataaaagaa tagactgcat tcgataaccg atcacgtaat ttcaattttt
    13861 ttcaccgcct ttctatttat ctatagatgg aacttaggta atcatctaac tcatctgaat
    13921 caatatctgt catttgattc atcgtttcct tatgaccaca caaaaacgcc acgaacccat
    13981 caatcttttt ctttgattga cgtttacttg gcgctttttg tccattgatg ttagtaattg
    14041 ctacaacgtt caaagtgcaa taaaggaata atggattatc aaattgaatt cgtttctcat
    14101 aaaacaaccg ttcgacatcg tcaaaaggag cattcaatac tttaggatat tgagcaactt
    14161 caacgcattc caatcctaag ttttctaatt tctcaacaag tttgtcactc atcgctggat
    14221 cataattcac ttgctgaata tcatataaat tcatgcagtc ctctatgtac tgcaaaatct
    14281 gatcttgatc aatcattttt ccgtcgcaaa attcaacgaa accttgttca gccaaatcac
    14341 tgtaaggaac attatcttct ttttctcgaa actctaaatc ttcattcgga ataaaataaa
    14401 gttgctttac tttaagaacc gctttaccat cttcatccca tgttgggaag tttaaagata
    14461 cacaggtcaa atctcgtgta cgtgataaat ctaaaccgat ataacatggt tcgccactta
    14521 aatcaccaag ttcttgtgtg gtaactaaac atggctctac ttgatcctgt tcaaagaaat
    14581 tatccgcgcc gttcacaaat acatccaagt gtttcgttag aaattcagct ttggaatgag
    14641 ctgaccgttg cgctgtttta aatgccgatt ctaaagcaga taaatcgact gatattcccc
    14701 agttaggatt gcacatttcc cagacttttc tatccgtcca atcataattt ttatttggct
    14761 catagattag aacaaagttt gaatcattat catcacgttt caacacttct tttgcttcgc
    14821 gatatacacg cattccaaca gacgacgaac ctttcccagc agttgagata ttaaacatca
    14881 atggctgtgg taaagatatc tgagcagact taaaattgtc atactgttcc attttctcct
    14941 gtttatgcaa ttcatcgttc aatacaaaat atggattgga tccctcgata ttatcaatat
    15001 tcttggtttg aacaataaat ttatttgagt aagccatatc ttcatgaata tagtcataag
    15061 taatactaga aacagttccc tttggccccc taaatatttt agttccttca agtaggattg
    15121 gattgtttaa gattgtaact gcaaaaggtt tggcagcata ttgcgcttga gcaaaatcag
    15181 atgcgcaagc atagcaatca actgataacg ccccttctcc gtacattgca tatcctaatg
    15241 aaccgacagc aatcaatgtt ttaccattct tttttggaat ctggacataa gcctcacgag
    15301 taacgcgtac aatttggcct ttttcatttt ctttaaccca accataaatc caagaataaa
    15361 taaatttttc ccagggttct aaaagaaacg gttttcccac catttctcct tttgtgtgaa
    15421 caataaatga ttctacccaa tccatcattt cattcgcgcg atctacatca aaccaaatat
    15481 cttttctttt tttccactga taccagcgat ctattgctaa acgaacggtc ttaggatact
    15541 tcttaggttt ctttcttact tcttttgcaa ataaatcagc ataatttaca ccaggttcaa
    15601 tcatttttca gcacctgcct tcttacgcca tttatttctg tgcgctgcta gttcatctac
    15661 tggctttact tctgggcgtg taatttcttc atcttttctt gcggtcgatc ctccagcaat
    15721 tagtctacca gtttttgctt tgtttgttaa tcctaataaa tctagagctt tcatcttctt
    15781 atctgcccaa gtttctactt gttgtgccaa tggatgtttt gattgattag tagctcctga
    15841 tttattcgtg aatttttgcg tctccggaaa tcctttttcc ttccacaata aatatttgtg
    15901 ttggtaaatt tcaaaaatat ccaagtatga ttcgattaat ggatcaagag tgatggtgta
    15961 caaatcagac aaattcatta tttctaagat acgagctttt tcagcactta ctttttcatc
    16021 aataatcgct ttacgttgcg ctttagtcgt catttttgta tacacccccc tttgtttttt
    16081 gaaaaatttg acctaacgat gcgcgtgact ccccgctacc ctatctcccc acgcgaaaaa
    16141 attttgaaaa tgaatagggg ggcttcaatt gaaatacgaa ggaaaaactt ttttgtcttc
    16201 cgtttcgttt tcaacaattg gatgacattt tgaacataat agcatgagat tgtttggatc
    16261 aagcttaagc agttcgttgt ctttgattgg tacaatgtga tggacgtgtg ctctctttcc
    16321 aaatatgaac tgaccacacc gctgacaatg accaccttct ctttcataaa taaattgacg
    16381 catatctttc catgcttgcg ttcgatagaa tggtttgttc tcatgatgat aaacagactt
    16441 tgcttgctgc ttcttcttgc gtgatctgct tgatctcttg tgttcagtac agtagatacc
    16501 ctttgctatt ttgttcgtgc atccgtcaaa ctgacagtat ttcattctac ttcacgaata
    16561 agatttatga tatcgccttt tgcacggata gcaccaggaa tatcaatacc atgtttctta
    16621 gcatatgcac gcaattcttt tgcagtcata ttgtccagtt catccgtatc ttcagtggac
    16681 tgatcattag tagcttcgtc tccatcaaaa ttagcagcag tgtttccatc gctatcaaga
    16741 atttcactgc tatgagctat gagttcgcca ttgacagcaa caaatgattt gccatcactc
    16801 aaaattcctt tttcttcttt tacttcaaaa tttggttctt gatctttcgg aacaactact
    16861 gtccttttct tttctgaatc ccaatactct gttccactaa tggatgttct aattttgatc
    16921 attgccattt taattctctc ctttgtaatt tgtacggatt acttttgcgc ccattcgctc
    16981 ataccattca atcgtttcct gcagattggg tagagtgtgt gacaataaag aaataactaa
    17041 cgtattcatt ccagttctgt aatcatcatt ctccacttgc acatgtgcat gattaccatt
    17101 ccaattttct ctgtacggac tattctctgc ttcaattgct ttcttataaa ctttagccaa
    17161 acttgattct acttcaatac taagtaccgc ttcatgaaaa tcattcattt gtttactctc
    17221 ctatcagata ttttgtggta tgatttataa aaaagatgcg aggtgtttta atgcttcaag
    17281 aagttaatat taagtatcgt gatcgtcaaa atacagagac aatttatttg ccaaatagtt
    17341 gtccgcattg cggaaatata atgactccgt atgtgtattt tggggtttca tcatatagtg
    17401 cgttcgataa tgatagaatc tttggcgtat tagtacagtg tataaatgat gagtgcaaca
    17461 aattttatag tttacaattt ataaatacgt ttaatgaaga tgaaggatat tctgaaaata
    17521 ttttaattaa agattattac cgtccgccga ttaaaataga tttaccagaa aatattgaaa
    17581 aagtttctcc tacatttgtt gatatttata ctcaagccac acaagctaaa tctgagcaat
    17641 tggaacaaat tgctggagtt ggttatagaa aagctctaga atttctaatt aaagattatg
    17701 caatatctaa aaattcagag gatgaagata aaataaaaaa aatgttatta ggtaaaacaa
    17761 ttgaaaatta tttgaatgat tttcctaaac tccaaagatt agccaaagct gctacttgga
    17821 ttggtaatga tgaaacgcac tatgttcgta gacatactaa taaagatgtt catgatatgg
    17881 atgcatttat taaatcagca gcgcaattta tcgcggctga ttatgatgct gatttagctg
    17941 atcaatttat aaatggaaat aactttatgc caactactaa ttaaaagaat aaaagagcca
    18001 cagataccaa ttaataaaac ggttgggata tcttggctct ttcatatttt tttgacacta
    18061 atagaatatc atgctgctaa caatgtttac atagctacaa tgaccgaaca tttagtgaac
    18121 agtgtaattt tagaataaag agagttgttc tttgtacttc tcgctattgt aaaaatcaat
    18181 ttcgttttct tttttcaatc ggtcagcttg tctgtgttca tactcatcta aaaagttcaa
    18241 tgtctttcta atttctgcat gtcgctgtct gacatacgat gagctatacc caacagattc
    18301 agcaatatct tctaagctca tttgatcaac atatttcatc ttaacaattt gattatcgat
    18361 accagaaaag ctatctatca gcaataacat ttcttctttt tgctcgatca acaattccaa
    18421 ttcattttct atcttttgga tattctcttc taaagaagaa gttctcgaat tcttttcgat
    18481 gcggacgttt gctaaatcac cacagaccca tcgattcaat tcaagcttac ttttattaag
    18541 attccacttt aagtaaagaa tctgctcatc tagttcttgg taatctttta accaatgaaa
    18601 tctcacaaac gccacccctt atggtagaat agtattgcgc tgctatctcg aaaggggtag
    18661 cttttttatt ttttcttagc cgttccattc tggattgcct tttgttccaa acgtcgtttt
    18721 ttcttcttaa tctttgattt tgtttttccc atacaattct cctttccgaa aacacatttt
    18781 gttcgatttt taatactaaa taaaagttgg ccagaatttt tcgttcgtct ttactcatct
    18841 ttttttgaat aagattacta gcataaaaat agttagtacc acgaaaatga acaggtaaat
    18901 caattacgtt caccttcttc atgccagtta tcagccacta gaatctttgc gttagtgccg
    18961 ccaccgtttt cttttttgta tttttcaaat gctttataag cagctgtttt tgatttatag
    19021 atttttccgg aaccgactgg gaacatccat gtgccctctg tgatagctaa gatgtatact
    19081 ttcatttaat tcaccccttt aatccccaac actacatatc cggacttctg catgtaatca
    19141 gtaatataag tgatctctgc tttgtatgag tcaccagtgt aactaaatgt tggtctcgtt
    19201 agtccaggta attcatgttt aatgtatttt ttatattcac gtagtattaa ctgatcacca
    19261 actttgaagt cacgatcatt cttgcggatc tcgaattgtt tgcgtccgct cgtcactgct
    19321 tcaaaatatt ctggtagtat ttttagttcg tggatcattc tgtttcctcc ttcaaaattc
    19381 gctcatcatc aaaaagtgaa tctccacaaa atccttctcc caaatattta tcgttcatgt
    19441 ataagtcaac caaatatttg tgatctgctt ctcgatattc attaatatta acgatcttta
    19501 ttttgtaaac attaccttcg tcgctcacta tcttgaattg ttctctgact ttcaccaccg
    19561 ttcctcctct ttttgaaaaa attttagtgt ttttgtaaga ccccctaaat cagctttttt
    19621 atttgatata atacttgtat cgattaatca aatcgaaatt aaatttaaaa aggtgattta
    19681 tcatgaatat caacaattta gtaaaactcg ttgaacaacg ttttaaagaa tcaggattag
    19741 aacaattaaa aaaagataat tctccagaat tattttctaa ttttaccgaa gacgacattc
    19801 ctactcttaa acaattaaaa tattattctg attattggac gcaaactcgt gagaatgaat
    19861 taaatttaga aaaactcatt attaaaaaga aatagcaact ccagcccaca cggctggttt
    19921 tttagtttca acttccctcc ttacgggtca aaatcatcgg ttccggcata atctgatcat
    19981 cctttagcaa caaatcagga tattttttcc ttagcttctt attgttcgtg taagttttca
    20041 atagccattg gttggcttcg cttttgcttt ctccagaaaa ataaattttc cctgtttgga
    20101 tgcctgcaag ttgaatcatc attttcacct caaatcaaat tttttctgct agatggagca
    20161 aaagcaatcc gacatcaaga atcttttgac ccattatttc agctactttg ctcggcttaa
    20221 ctccttgaga aaagagcaag cgtgcttgct ctatctcttc gtgagtccaa ataaatttgg
    20281 cttcctcaag gataattact ggatcagttg ctctcattgt tcctctggaa aaatgatgtc
    20341 attcttatgc ttggaccagg tatctgcata tggaacaaag tattgtctag ctaattcgat
    20401 ttgatcaatt aaagcagttt ctgataattc atggtctgca gcaatttcat tcatactatc
    20461 gccttcatct aaacagataa gaccgttgac gttatcgatt tttacatttg tgactgcttg
    20521 actttctcca cgctcataat attctacagt tgatactgat acatcagctt cggagtttaa
    20581 ttgcagaatc tgttactgcc gggataattt aatattcttt tcataattca ccttttttcc
    20641 tactgacatc gaatattttt catgttatga ttagccgtga aaggagagct atattatgtg
    20701 tttacacatg atgtccttag ctcattcggt tctttggaag cttttttgga aataactctc
    20761 aggttcttga cgcaaatctc atttattctt tgaaacaaaa aacttccatt ttccgagagc
    20821 tttggatcac ttcaaaagag atttgcattt atatttctgt gaccttgagt cgcgtaaaga
    20881 accttacagc aatggcggca attccacttt caggaaaccg ccttttttca ttgatattgg
    20941 tggttagcgg actacccatt ttctttcggc agtgccaaac cacaaatgat ctgtgttgat
    21001 gtttaggtag ttgtatacca gataagctaa atccaaagtg cctgcatctt caagacgaca
    21061 atattccaaa aattctccca tagctggaca taaaaatcct ggagctatca tatcccaaca
    21121 gttaatagtg caaagaaatt cgtttagctt ctgaacgttt tctttggttt cacgaattac
    21181 aacatactcc aattcttctc cgctggcaga gcattccaaa acttcaaatt gattaagaaa
    21241 tggtagaaca tatgcttcaa acatcaacat tccatctttc tgtttttgca cttgttttgc
    21301 atcaatcatg tctatcctcc tattcagtta tttcttccat taatggaatt atttcaggtc
    21361 actggactta acgaaaacac cattgaccat ctttccagtc cgacctttga tttcgtcata
    21421 agcaaaatcc aagcactcgt ataaatccat atcattttgc attgccaaga tgatcaaggt
    21481 tactactaca tcgccaattc cgtctcttaa accatgttca tcttttctag ctagagaagc
    21541 ggcaacttcc ccgatttctt cgaccgtttt taacatttgc ttgctgaaat ccgcttgatc
    21601 caatccctta tcttttgccc actgctctac tttcgcgatt agttcgtcca ttatttctcc
    21661 tccacatacc taaactgtct tccttttgaa tcaatccata agctcctagc tctatcccaa
    21721 ataatatttt tgctcagacc agtaatttca gataactgtt tagcggtacc cgttactaga
    21781 attcgatcac catgccagat tgcaatcttt ctcggcgttc tccgtttggg cttttcagtc
    21841 cacattgatt taccgagctt ttggacttct gcaactattt ttttatcttc ctgccaatct
    21901 tctgaataag tcaattcgat aattcgctgc attgccgctt tcttatccat cccgacattc
    21961 tcctttcagt aatttgagta cttgatcaag tgcgctctca cgtccgccat gaaatgtgtt
    22021 gagccacttg tcttcgtacg acacactttg tcttaaagct tcttgatgca ttaattcgat
    22081 ctgtgctgta aatgtcttta ggtccatctt gtttacacct gctcaagttc attaagatgt
    22141 ttttgcaagc ctttaacaca atcaatgaat agtaatttgg tatatgctaa atttcttaat
    22201 tgcgttccat cgatatagag tgcgaaatag tatctgagtt tactccaact cgaacgatca
    22261 ttcttaattc cttcaattcc agcttcttcg agttgatcat atacgtctct caggacgtct
    22321 aattcctcgc cctttttata attcgctatt tcattaatta gttctagata atcgatcttc
    22381 atttttccac ctcttagaat ggtgcttttg attgtctatt agctcgttct agcgcttttt
    22441 tcttgagata ggcttcttgg tcgattgccc attcaggaag cttctctcgt cttcctgtgc
    22501 gcttgtatcc actgcttgcg ttcttaggtt cacttttttc tttccttgcc caacttcgaa
    22561 tagttgccaa atagttttta taagtcttac cagatgattc acaatactct gacagtcgtt
    22621 cgattcgctc ttggtaatca ttagggaatt ctgttttgag tttctccatc tgctcatctg
    22681 acaaaagaac atttttatac tctccgtatt tatgacggac gggcttagcc ttcgattttt
    22741 tcgaaggcgg taactctctt atatattctt ttgtattatt aaatgtatta ttaatagatg
    22801 tattattatc tttgactttt tcgtcaatag gggtattgcg tttttcgtca atagggtatt
    22861 gattaattcg taggtaccta ttgattattt gattggtacc ctctttgtaa atgatttccc
    22921 gattcaagta tccaaactta atcaaatcac ttacccatcg cgatatggtc tctttattca
    22981 caccatataa atctgcaaag tactcattgc ctgcccaaca aaagcctctt tcattacaca
    23041 aggccgttat ctctccgtat aacaacttag tatttggttt aagtcttttg tcgtacctta
    23101 cgttggctgg tataatcgca taataacttc gatgttctgt catttttccc ctccaatatt
    23161 taactttttg attgttttct ggtttaattt gatccctttg atttgatatt tatttttgaa
    23221 attaatcaca cctattttgt gcttctctgt gtgatggatt ctgcagagtg ctgcaaatgt
    23281 gtactctgca tgatcaactt ctttgcgctt tcgtcttcct agcgctttgt caaagtgatc
    23341 gatgtcagct cctgttttgc cacagatgca gcagactctt tttgtgatgc atttgtagaa
    23401 gtaatattct tgattcgcag gtaaaatctc atagccttct ttgaaaggaa tatgatgttc
    23461 aaagatgaaa tctaagatga tatttgctaa gacattggca tcactcacag ttgtattcga
    23521 ttcgtctttc aggcttattt tgcgccctgt gacaccttca aaacgaaagt agaagaattc
    23581 cttccagaag tccgttggca tgcctgtatc gataaaaata tcgcctatca gcgcatagat
    23641 gaagtttcgt tgctgtac
//
LOCUS       DAYYAO010000043        23653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000043 DAYYAO010000000
VERSION     DAYYAO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.045"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..506)
                     /locus_tag="ACHARI_001598"
     CDS             complement(<1..506)
                     /locus_tag="ACHARI_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8442648.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="ACHARI_001599"
     CDS             779..3250
                     /locus_tag="ACHARI_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8442649.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="ACHARI_001600"
     CDS             complement(3353..5089)
                     /locus_tag="ACHARI_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8442650.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="ACHARI_001601"
     CDS             complement(5086..5790)
                     /locus_tag="ACHARI_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8442651.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="ACHARI_001602"
     CDS             5921..6424
                     /locus_tag="ACHARI_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8442652.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="ACHARI_001603"
     CDS             6384..6722
                     /locus_tag="ACHARI_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8442653.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="ACHARI_001604"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="ACHARI_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8442654.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="ACHARI_001605"
     CDS             8273..8851
                     /locus_tag="ACHARI_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8442655.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="ACHARI_001606"
     CDS             8855..9376
                     /locus_tag="ACHARI_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8442656.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="ACHARI_001607"
     CDS             complement(9455..10009)
                     /locus_tag="ACHARI_001607"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8442657.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="ACHARI_001608"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="ACHARI_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8442658.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="ACHARI_001609"
     CDS             10549..10800
                     /locus_tag="ACHARI_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8442659.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="ACHARI_001610"
     CDS             10925..11716
                     /locus_tag="ACHARI_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442660.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="ACHARI_001611"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="ACHARI_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8442661.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="ACHARI_001612"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="ACHARI_001612"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8442662.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="ACHARI_001613"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="ACHARI_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8442663.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="ACHARI_001614"
     CDS             14014..14676
                     /locus_tag="ACHARI_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442664.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="ACHARI_001615"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="ACHARI_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8442665.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="ACHARI_001616"
     CDS             18096..18410
                     /locus_tag="ACHARI_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8442666.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="ACHARI_001617"
     CDS             18423..18797
                     /locus_tag="ACHARI_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8442667.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="ACHARI_001618"
     CDS             18807..19151
                     /locus_tag="ACHARI_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8442668.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="ACHARI_001619"
     CDS             19222..20574
                     /locus_tag="ACHARI_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8442669.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="ACHARI_001620"
     CDS             20634..21776
                     /locus_tag="ACHARI_001620"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8442670.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="ACHARI_001621"
     CDS             21801..22055
                     /locus_tag="ACHARI_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442671.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /gene="mobT"
                     /locus_tag="ACHARI_001622"
     CDS             22311..23495
                     /gene="mobT"
                     /locus_tag="ACHARI_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8442672.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23492..23629
                     /locus_tag="ACHARI_001623"
     CDS             23492..23629
                     /locus_tag="ACHARI_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8442673.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7832 a         4219 c         5072 g         6530 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacctta
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DAYYAO010000044        23417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000044 DAYYAO010000000
VERSION     DAYYAO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.046"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..1698
                     /locus_tag="ACHARI_001624"
     CDS             37..1698
                     /locus_tag="ACHARI_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8442674.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1768..2115)
                     /locus_tag="ACHARI_001625"
     CDS             complement(1768..2115)
                     /locus_tag="ACHARI_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8442675.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2199..3140
                     /gene="opp3b"
                     /locus_tag="ACHARI_001626"
     CDS             2199..3140
                     /gene="opp3b"
                     /locus_tag="ACHARI_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8442676.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3141..4184
                     /locus_tag="ACHARI_001627"
     CDS             3141..4184
                     /locus_tag="ACHARI_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442677.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4190..5260
                     /locus_tag="ACHARI_001628"
     CDS             4190..5260
                     /locus_tag="ACHARI_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442678.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5250..6203
                     /locus_tag="ACHARI_001629"
     CDS             5250..6203
                     /locus_tag="ACHARI_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442679.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6215..7225
                     /locus_tag="ACHARI_001630"
     CDS             6215..7225
                     /locus_tag="ACHARI_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8442680.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7510..8331)
                     /locus_tag="ACHARI_001631"
     CDS             complement(7510..8331)
                     /locus_tag="ACHARI_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8442681.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8428..8706)
                     /locus_tag="ACHARI_001632"
     CDS             complement(8428..8706)
                     /locus_tag="ACHARI_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442682.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8974..10983
                     /gene="metG"
                     /locus_tag="ACHARI_001633"
     CDS             8974..10983
                     /gene="metG"
                     /locus_tag="ACHARI_001633"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8442683.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11462..12049)
                     /locus_tag="ACHARI_001634"
     CDS             complement(11462..12049)
                     /locus_tag="ACHARI_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442684.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12224..13546
                     /locus_tag="ACHARI_001635"
     CDS             12224..13546
                     /locus_tag="ACHARI_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8442685.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13701..14477
                     /locus_tag="ACHARI_001636"
     CDS             13701..14477
                     /locus_tag="ACHARI_001636"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8442686.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14470..15039
                     /gene="rnmV"
                     /locus_tag="ACHARI_001637"
     CDS             14470..15039
                     /gene="rnmV"
                     /locus_tag="ACHARI_001637"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8442687.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15032..15916
                     /gene="rsmA"
                     /locus_tag="ACHARI_001638"
     CDS             15032..15916
                     /gene="rsmA"
                     /locus_tag="ACHARI_001638"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8442688.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16367..16996
                     /locus_tag="ACHARI_001639"
     CDS             16367..16996
                     /locus_tag="ACHARI_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442689.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17120..18229)
                     /locus_tag="ACHARI_001640"
     CDS             complement(17120..18229)
                     /locus_tag="ACHARI_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442690.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18438..18860
                     /gene="mgsA"
                     /locus_tag="ACHARI_001641"
     CDS             18438..18860
                     /gene="mgsA"
                     /locus_tag="ACHARI_001641"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8442691.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19047..19145
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19183..19719
                     /locus_tag="ACHARI_001642"
     CDS             19183..19719
                     /locus_tag="ACHARI_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8442692.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19759..21522)
                     /locus_tag="ACHARI_001643"
     CDS             complement(19759..21522)
                     /locus_tag="ACHARI_001643"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442693.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21525..23240)
                     /locus_tag="ACHARI_001644"
     CDS             complement(21525..23240)
                     /locus_tag="ACHARI_001644"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442694.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7543 a         4289 c         4770 g         6815 t
ORIGIN      
        1 aattattctg aaaattccaa taaaggggta cgtaaaatga aaaagaaatt tacatttggt
       61 ttggttgcgg tatgcggcct tgtacttgct ggatgttatg gcggcagttc agccacaagc
      121 agtagttcag caaaaagcgg ttcagcagat ggaggaggcg tatttaactt agtcgtgcct
      181 caagaaatgc ctacagctga cttatcagtt gcaacagata caatcagttt tacagcattg
      241 aataacgttt atgaaggaat ttaccgtctt gatgaagaca gcaaaccaca gccggcagga
      301 gctagcgagt tggcagaagt cagtgaagac ggattaactt acaaaatcaa attacgagaa
      361 gacgcaaaat ggtcaaatgg tgatcctgtt acagcagcgg attacgttta tggctggcaa
      421 cgaacagtag acccagctac agcttctgaa tatgcttacc tgtatgcgcc agttgaaaat
      481 gcagaagaca ttacagctgg aaagaaagat aaatcagaac tagggatcaa agcagttggt
      541 gactatgaat tagaaatcaa attgacgaaa caaacaccat atttccaata tctgcttgct
      601 ttcccatcat ttttcccaca aaaacaatcc gtagtggaag aaaatgggga tgcctatgca
      661 tctgcaagtg ataaagctgt ttataatgga ccattcacat tagaagattt tgatggacca
      721 ggaacagata cagaatggac ttataagaaa aatgatcatt actgggacaa agatacagta
      781 aaactttctg aaatcaaagt cagtgtagta aaagaatctt ctactgcatt gaacttattc
      841 aaagatggcc aagcagatga cgtgatcttg tctggtgagt tagcacagca aaacgcaaac
      901 gatccagctt atacttctgt taaagaagcg cggacaagct atatcgaatt caatcaacgt
      961 gaaaagaatt caccattcaa caatgtgaac ctaagaaaag ctatttctta ttctatcgat
     1021 cgtgaagctt tagtaaaaca agtactaggt gacggatcag ttgtctctac aggattgatc
     1081 ccatcagata tggtaaaaag tccaacaacg aacgaagact ttgcaaaaga agctggaaaa
     1141 ttagtttctt atgacaaaga taaagcaaaa gaatattggg aaaaagcgaa aaaagaattg
     1201 ggcgttgatt ctttagaatt tgatttgatg gcttctgatg atgattcatc gaaaaaagta
     1261 atcgaatatg ttcaaaactc aatccaagaa aacttggatg gcgtaacagt caaaccaaca
     1321 cctgttccat tctctgtacg tcttgaccgc tcaacttcgg gtgattttga cgcagtacta
     1381 ggcggatggg ctgctgacta tgcagaccca agcagcttta ctgatttgtt tgtcacaggt
     1441 aattcataca accgtggtcg ctggagcaat gcagaatatg acgaagcagt taaagcagct
     1501 tcatcaacag acgctggcga tgaacaagcc cgttgggatg atttccaaaa agcaatcaag
     1561 atcatctctg atgatatggg tgtcgttccg gtgtaccaaa aagcagaagg tcacctagtg
     1621 aatgaaaaaa tcaaaggtat cgttcaccat gcggcaggcg cttcatggga ttacaaatgg
     1681 acatacgttg aagaataatc gttaataccg tgagcatgag aaaaaacgag gctgcggcaa
     1741 atgtcgcaac ctcgttctca tattcactta tcctaacaaa gaaaaaagca tgaaaatagc
     1801 agaaagagca attagtattc cggcaatgat cagccaaaag ctgtacatac cgtagctggc
     1861 agacgagagc gtggaaaatt ctggtttttt ctttcggttc cttgttcgtg caagatggac
     1921 cgatcgctga aaatgatcga agaaacccga agaaaagacc cataaaaaac cgccgataat
     1981 cagaaacggc aatgaccata agaatagatc attagataat tgaatgagtg taaaggaatg
     2041 gttgatgaga ttttttatta aaataaccag aatcagtaga aaagctactg cgtaggggat
     2101 agattttttt ttcatttgat tgctccttta tttttggttc attatctaat aatgtagcga
     2161 aacatctgtc agaagtcaaa agctaggggg aaacattagt gaatagttat atcaaatatg
     2221 tattaaaacg tgtctttttc atgatcatca cgctttggtt aattgcaaca atcacctttt
     2281 tcttgatgca attgctgccg ggcacgccat atacgaatca ggaacgtttg agtcctgaaa
     2341 caatcgagat gctaaataaa caagtcggtt tggataaacc agttatcgta caatatggga
     2401 tttatctttc taatttgctt caaggagatt ttggaatttc cttccaattt aaaaaccagc
     2461 cagtcgcaca tttgttagct ggaagaatcg gaccatcctt gcagttaggg ttacaggcaa
     2521 ttatctttgg aacagtccta gggacgattt taggaacgat ctctgccatg aagcaaaata
     2581 catgggcaga tacatcttct acactcgtag cgatattagg tcgttccatt ccaaactttg
     2641 tatttgctgt tttattacaa tatatctttg caatcaaatt acaagttttg ccaatcgcaa
     2701 aatgggatgg ctttgtgtac acaatcttac caacaattgc gttggccatg tcgccgcttg
     2761 ctgactctgc tcggtttatc cgtacggaaa tggtggaagt actgcatagt gattatgtgg
     2821 aattagcaaa agcaaaagga ttgagccgtt gggaaatcgc ctttaaacat ggtcttcgaa
     2881 atagtttgat tccattgatg actttattag gaccgttagc tgtagcattg atgaccggat
     2941 cactggtggt agaaaatatc tttgctattc ctggtatcgg ggaacaattc gttaagtcaa
     3001 tcacaacaaa tgattatcct acgatcatgg ctgtaacgat cctttactca tttatgctga
     3061 tttttgttat tttgatcgtg gatctattgt acggtctagt tgatccaaga attcgtgttt
     3121 cagaagggag ccgaggataa atggaaatga taccaaagcg ttatgaaaca gttgcagata
     3181 tccctgcgga tgaatttctt ccattacaaa aaaataccga agaagaacgt gaacagatta
     3241 ctgcaccttc tttgaatttt atccaagatt catggcgccg tttaaagaaa aacaaagctg
     3301 ccgttatttc aatggtttta ctattggtca tcattttgat ctctgtgatc acaatctttg
     3361 tttctcctca tgatccaact gcccaaaacg tggcttatat caatttgcct cctcgtattc
     3421 cgggaatcaa tatcgatgga ttgaatggaa aagcaatggt tggtggtgaa cttgttgata
     3481 aatatgctca agcaaacgtc ccttcaaacg taaatttctt tttaggaaca gacggtttgg
     3541 gacgtgatgt attaagccgc ttatttatgg gaatgagaat ctcgttgctg atcgcattta
     3601 ttgctgcatt actagatgta acgattggtg tagcttacgg gttggtttct ggtttgttag
     3661 gcggacgtgt cgataacgtg atgcaacgtt ttttggaaat cttatcagga attccaaacc
     3721 tcgttgtcat gattttgatg ttggtcgttt ttgaaccagg aattttttca atcgtggctg
     3781 ctatggccat cacaaactgg ataccaatgg cacgtatcgt tcgtgcacaa acaatgaaat
     3841 taaaagatca agaatatgtt ttagctggat tgacattagg tgaatcaaga atgaaaattg
     3901 cattcaaaca tattttgccg aatatctcta gtgtcattat tattcaaatg atgtttagta
     3961 ttccatctgc tattttcttc gaagcctttt taagctttat cggtttaggt cttacgcctc
     4021 cgtcagcttc attaggaacg atgttgagcg aagggtacaa gacattcctg tatctgcctt
     4081 atctattatg gattccggca gtaactttat cggttatcat gattggcttt aacttacttg
     4141 ctgatggatt gcgtgatgcc tttgatccta aaatgaaaga gtgaagacga tgacgaaaat
     4201 tttagaagtg aaagacttag aaatctcttt tgatacgtat gctggaaagg tacgagcgat
     4261 tcgcggtgtc aattttcatt tgaataaagg agaaacattg gcgatcgttg gggaatctgg
     4321 aagtggaaaa tcagtgacga ccagaagcat catgcgcttg ctgtcaagca acgccaatat
     4381 agatgctggt caaatcctct ttaaaggtaa cgatatcgtt ggaaaatcag aaagagagat
     4441 gcaaaaaatc cgtggtaaag aaatagcgat gattttccaa gatccaatga cctctttaga
     4501 tccgacgatg cccattggga agcaagtcgc tgagtccctt agaaagcata ataaagtttc
     4561 aaaaaaagag ggacaaaaag ctgcattaga attgcttaaa ttagttggaa tacccgaagc
     4621 tgagaaacgt atcaacagtt atccgcatca gttctcagga ggtcagcgtc aacggatcgt
     4681 gatcgcgatt gcactggtat gttatccgga aatcctgatt gctgatgaac ctacaacagc
     4741 gcttgatgtg actattcaag cacagatttt ggagctgctg aaagaaattc agacaaaaat
     4801 cgatacatca attatcttta tcactcatga ccttggtgta gtagcgaatg tcgctgatcg
     4861 tgtcgctgtc atgtatggag gaagaatcgt tgaagttgga acatcggaag aaattttcta
     4921 caacccgcaa catccctata cttggggact gcttggttca atgccgacat tagatagtgc
     4981 gaacgatcga ttgtatgcta ttcctggttc tccaccggat ttattgaacc cgccaaaagg
     5041 agacgcattc tatccaagaa atgaattcgc gatgaaaatc gatgcagaac aagaaccacc
     5101 attttttgaa ctatcaaaaa cacacaaggc agcaacttgg ctacttgctc cgcaggcacc
     5161 caaagtgaca ccgccagaag aaatccaacg tcgctgggct atctttaaag agaaacaaaa
     5221 acatacttac ggtcaaggag gaattgctga tgccaaataa actacttgaa gttaaaggat
     5281 tgaaacagta tttcaatgta ggaaaaaaga atgaagtaca tgctgtaaat gacatcagct
     5341 ttcacattta tgaaggagaa acattcggtt tagttggaga atctggaagc ggaaaatcca
     5401 ctactggccg tacgatcatt cgcttgaatg aacctactga tggtgagata ttattcgacg
     5461 gtcaagatat aacgaagatc aaagataaaa aaggtttgac gaaattccgg catgatgttc
     5521 agatgatctt tcaagatcca tacgcttcgt tgaatcctcg tatgaaagtc agagatatca
     5581 ttgccgaagg aattgatgtt aatggactag ctaaatcatc tgaagagcgt gcaaaaaaag
     5641 tcgatgatct attgagaact gttggattga atccaagcca cggtactcgt tatcctcatg
     5701 aattttcagg tgggcaacgc cagcgtattg gtattgcacg agcattggca gtaaaaccgc
     5761 gttttatcat ttgtgatgaa ccaatttctg cactagatgt gtcgatccaa gcccaagtgg
     5821 tcaatttgtt gcaagatctc caaaaagaac atcaattgac ctatctattt attgcacatg
     5881 atttgtcgat ggttaaacat attagtgacc gaatcggtgt aatgcataac ggcttgttat
     5941 tagagatggg aacaagtgat gaaatctata atcacggtgt tcatccttat acagaaagtt
     6001 tgttatctgc tattcctcta ccagatccag accatgagag aaaacgcaga agaatcaaat
     6061 atcaacctga accagatgac ggacaagtac gtcaacttcg tgaaatcgca ccagaacatt
     6121 ttgtctatgc aactgagcag gaagtcccat attacgcaaa aaaactaaaa cgacaaaagg
     6181 aatctcttct ggttgctaac taggagggac atagatgcta aaagctttta aattccgtat
     6241 gtatccgaca gaagaacaaa aacagcaatt gattcgtact tttggatgtg ctcgatttac
     6301 ctataaccat ttgctgaaaa agcggcagaa aagttggcaa caaacaggag tagccaattt
     6361 ttcgctcaca cccgctacat taaaaaaaga atacccattc ttaaaagaag tagacagtct
     6421 tgctttagca aatgcacagc ttaatttgga tcgggctttt cgcaattatt tcaagggacg
     6481 tgccagcttc ccaaagctta aaacgaaaaa aagtatgtgg caatcttata cgaccaacaa
     6541 ccaaacccgt acgatctatc tggaaaatgg ctacttaaag ttaccaaaac aaaaagaact
     6601 aatcaagata aatcagcatc ggccagttga aggatcgatt cgctccgcta cgatttctgc
     6661 tcgatataat gaagaatttt atgtcgcact actttgtgat gtttcgccag taaaaaaaga
     6721 atctttagct aaatggatcg ggatcgccta tcatccgaag acattgatcc aaacatctcg
     6781 acctctagaa gtaactttac caaaatttca ccagacagaa gaaaagctgc aacatgcaca
     6841 gcggaaattg aatgtgaaag tccgctccgc tcaccataga aagatcaggt tagatcaggc
     6901 aagcaattat cagaaacaaa agagaaaagt aatggatctt tatctgaaac aaaagaacca
     6961 gcgagaagat tatcttgaac aattatctgg gaaattggtc aaacaatatg actacttatt
     7021 tgtagaatct tttccaaaag aagaggcaca tgctgatttt tctattcacg actggcataa
     7081 attaataaca aaattgcgtt ataagtccca atggtataat aaaaaatttc ttttgatcaa
     7141 taccgacgga gcagaagaaa gcaattccgt aagaaagagc caagtagtag aaaaaatggg
     7201 cagacattcg gttatcaagg gataaaaaat gaatgaaact gataggagcg ttagggttag
     7261 ccttgttaat aaaaagtctt aatgactttt gtttctagga atttttcttt tgaagaaaag
     7321 ctcactaatt atgacaaacc aataaaacaa tagcagaaaa agttcgtacg agctttttct
     7381 gctattgtta attttttatt cagaaagttt acgaatacat caaaattctg aaacaaaaaa
     7441 cgtaactcac aaagagttgt ttttgcgttt tttcttgaat gattaggtct gaatcttttg
     7501 ctcacaacct tattcatggt gccccggctt gagctgatag atttttgaag gacggccgat
     7561 tcctacagga cgttcaccga tctcatcgaa atattggagc atagctttct taaaattgga
     7621 atgatcgatc gttttataat cgatccctaa gaatttagca aatacttttc gagcttccgt
     7681 aatcgtgaaa tctttaccta aaacttgtag aacttgcggc tcgtgttcca ttttattgac
     7741 tacacggttg aatgctttca agatgatttg actatggtca aaagccaaag tatctttgcc
     7801 agaagactct ccagtgacta aatcaagcga gatagccact tctccgctag ataaatggat
     7861 cttgttacta tgacgttcta gtgtgaacca gcgtacttcc ttggcatcat ctccggcgat
     7921 tagcggttct tctccaataa aggctaagta gctgactgtg acgacccagc ctcgcggatc
     7981 tcgattaggc gtactgaatg tatgcagttg ctcaatattt tcttttgaaa taaccacacc
     8041 agtttcttcc tttgtttctc ttaagacgct ttctccagtt gactcatcct tttggacaaa
     8101 gccaccaggc aatgcccatg aatttcgata aggatgccct tttctttgga tcaacaaaac
     8161 tttcagctga tcttcttctt tgttatagca catcagtacg atatcgacag tcaaagaggg
     8221 cttgtcatat tctggacgtt cctgtttttg ataccagtta agaaattctt cttcacttgc
     8281 tttatgttca taatataatt tttcttcttc tttagaagca aaacggttca tctgaccacc
     8341 tccattgaca gtaaaagtac tttttacctt attgtagcag actctgaaaa aaaaccaagg
     8401 atttacttga aaaatctagg ttatcattta aatatgaaaa ataaatggaa gtatgagatt
     8461 gaataaaaag aaaagcagat aaaacggcaa tactttatac cgctcttcac gaatggcaaa
     8521 gaagccacaa ataacgatat atacgtcaat gataaaacgc aaaatccaag ctgctgtcat
     8581 aaaactgttc aaatgcccat ggaggttaaa ataaccatac atacgaatat aaaaatagct
     8641 gaatattaaa tgtgctaatg aaagaaaagc aatactaagt gtaaaaaaaa gatgtttctt
     8701 ctgcatgatt gttccagtcc cttactaaga tagatagtct ttattatacc acgtgtgagg
     8761 ggagaaataa aaagccgttt tttagtagat atgaaacaaa acaaaaaccc gcatttattc
     8821 aacctattca tccttttaaa ggatagattg aacgtagata gaagtcgttt atccagtctt
     8881 tttcattttg aaaaaaagct aacagcatgc tttttttatg gtatactagc aaggaaacga
     8941 attcgcatca tgcgataaag gaggaagagc cccgtggcag aaaaggaaac tttttatatc
     9001 actacgccaa tctattatcc aagcggcaaa cttcatatcg gcaactcgta tactacgatt
     9061 gcatgtgacg caattgctcg ttataaacgg ttgatgggct ttgatgtgtt ttatctcact
     9121 ggagtggatg aacatggtca aaaaatcgaa acaaaagcag aagaactagg tgtaaaaccg
     9181 caagaatatg tagataaaat ggcggctgat gtcaagaaat tatggaaaac gcttgatatc
     9241 agttatgata aattcattcg cacgacagat gattaccata aagcagctgt tcaaaaaata
     9301 tttgatcgtt tattagaaca gggagatatt tacttaggtg aatatgaagg ctggtactct
     9361 gtttctgacg aagaattctt tacagaaaca caacttgcag aagtgtacaa agatgacgaa
     9421 ggtaacgtga tcggtggtaa agcgccaagt ggacacgaag tagaattggt aaaagaagaa
     9481 tcatacttct tccgcatgag caaatacgca gaccgtctat tggaatacta tgaagaacat
     9541 cccgaattca tccagccaga atctcgtaaa aacgaaatga tcaacaactt tatcaaacca
     9601 ggattagagg atcttgcagt ttcacgaaca acttttactt ggggagtacc agtcaagagt
     9661 gatccgaaac atgtggtgta tgtatggatc gatgcattat ccaattatat tactgcacta
     9721 ggatatggct ccgaagatga aagtctattc gaaaaatact ggcctgcaga tgttcagatg
     9781 gtcggtaaag aaatcgttcg ctttcatacg atctattggc caatcatgct gatggccctt
     9841 gatttgcctc tacctaaaaa agtatttggg catggctggc tattgatgaa agacggaaaa
     9901 atgtctaaat caaaaggaaa tgtcgtttat cctgaaatgc ttgtggaacg ttatggttta
     9961 gatgcactcc gttattattt gcttcgtgct gttccgtttg ggtcagatgg tgtctttacg
    10021 ccagaagatt ttgtttcccg tgtcaattat gatctggcaa atgatctagg gaacttattg
    10081 aatcgaacga ttgctatgat caacaaatat tgtgatggac tcgttcctga ctatgcctca
    10141 cttgtcactc catttgatag tgaactgtct acgacggctg ctaacgttgt cggacgttac
    10201 catgatgcaa tggaaaaaat ggaattcaat acagcattgg ctgaaatctg ggtgctgatt
    10261 tctcgtgcaa acaaatacat tgatgaaaca gaaccatggg tattagcaaa agacgaagaa
    10321 aagaaagctg aattgaatag tgtcatgatc catttagctg aatctttgcg tatcgcagcg
    10381 atattacttc agccaatcat gacagaaacg ccagagaaga tcttcaatca actaggattg
    10441 gactctgaaa caatgaatct tgaaggatta cactttggcg aatttccttc aggaacaaaa
    10501 gtagttgcaa aaggtacacc aatcttccca cgtttagata tggaagaaga agttgctttt
    10561 attcaagaaa aaatgtctga aggtactcaa acaaacgaag atacagtcaa atgggatcca
    10621 gaagaaacag aacttgtttc aacgaaagaa aaacaaatca aatttgatgt atttgaaaaa
    10681 gtagaactaa aagtagcaga agtcatcaac tgtcaaaaag tagaaggggc agataagttg
    10741 ctgcaattcc gactagatgc tggagacagc caagaccgtc aaatcttgtc aggaatcgct
    10801 gaattttatc ctgatccaag cgaattgatc gggaaaaaag tcgtcattgt agcaaatctg
    10861 aaaccacgaa agatgcgtgg acaaatcagt caaggaatga tcctttctgc agaagcacca
    10921 gatggctctt tacaagtaat cgaagcacca aaatccatgc caaatggctc agaaatcgca
    10981 tagaaattca aagggggtat gacactagtt gtgtcatacc ccctttttcc gaaatttgag
    11041 aagcaatttt cgtaaattcc ttcttattac ttgaagccaa tcatataata cagaaacaga
    11101 gaactatcca aaataaatcc tagaaaaaga agtacgagct tcgctgattt ttgttaattt
    11161 agaataatac taataggaat cgtaaaaaca gaaaaaaagc ataatgataa agggtcgggc
    11221 agattttaat tgattcatct ggtactcttt tttattgtcc ttacattcat aaagtttgaa
    11281 tgcaatgact ttattacatc tatatttata tctaacagta gcgttgatgt gttaattata
    11341 agttctatca ctttatgttc aattagtttg tgctcttata tgtgtgattg aaaatataga
    11401 taaaaaaaca atcatcggct ttgacttcga tgattgtttt ctttattttg taaaaatcct
    11461 atcacttgat tttatggtaa ccttttattt ttgtcttcaa acggcttggt aagagagcag
    11521 taagcagtgc aaatgggatc gaaaagacat accagaaaat cgtacgtccg ataccataat
    11581 gttgatagag ctttgtcaac atcacgtgta acaagatcaa ccctaaaagc aaggagccag
    11641 ctataaacca atgattatcc caatgcatga cttcactaag tgccatcact ccaaaaggat
    11701 agaaataaaa atagatcccg actaaggtca tccgccagtt ctttgtaaat gttccgatat
    11761 tcaataaaaa gaaaatcgct gaaagcaaaa taccgatagg cggaaaaagc ccaaccaaaa
    11821 aggaatacaa catcccccac aagatcatca caggaccttt gatcaatgca acgattgcga
    11881 tgaacaaagt cagcaatatt agtttttgtg ataacgttgc aacagtcatt aatccaaata
    11941 aaattaatgt gaaccaatgc caatgggact gctgttgttc tttacgggaa aaatcgaccc
    12001 ggttggataa ataatctgct aatttcggat aagaacgctt aaaatccata aactgttcct
    12061 ccttcgtgga aaaggtaggt ttagtatacg aaaaataaaa aagaaataca tccgtcttaa
    12121 gagggagttt ttcactgaaa gtggagaaaa aagaatggta aattaaaata ttctcagtta
    12181 tactgaaaat tataccaaat ttcaaagaag aaagaggatg ggaatgaaag gcaaactaaa
    12241 acgagaaatc aatctgtttg gcgcattagc taccgttatg ggaaccgtga tcggtgcagg
    12301 cgtcttcttc aaaactgctg ctgttactgc tagtactcag tctacaagtt tgacattgct
    12361 agcttggctt ttgggtggtt tcttaacgat ctgtgctgga ttgacagtgg ctgaattagc
    12421 tacagcaatc ccggaaactg gtggagctgt aaaatatatt gaagctgcgt atggaaaatt
    12481 gcccagcttt ttattaggat gggcgcaaag cttgatttac tttcctgcta atattgctgc
    12541 attatcgatt atctttgcga cacaattgac gaacttatta caattatcta ccgattatct
    12601 tttgttgatt gcagtgatca ccgctgtttc tgtgacaggg ttgaatttgt tagggacaaa
    12661 agtcggtgct tctgtacaat cagtgacgtt gattgttaaa ttaataccaa ttgctgtgat
    12721 tgtcatatgg ggattattga ccccaggaca aggaactgtc cagttatttc caatcgaagc
    12781 tggaaaagat gttacttttg tcgaaggatt gagcagtgcg ttgttagcaa cgttgtttgc
    12841 ttatgacggc tggttaggtg tcggtgcaat ggcaggggaa atgaaacggc cggaaaaaga
    12901 tttgcctaaa gcaattattc ttggtttgag ttttgtaaca gtcgtttatc tattgatcaa
    12961 ttttgttttt ttgaaaactt tgccgattga tcatcttgcc ggtaatttga atgcagcttc
    13021 tgaagcatca gacgtcattt ttggaggaat cggtggaaag ctggtcacta ttgggatctt
    13081 gatctcggta tacggtgcat tgaacggtta tacactaact gggatccgcg tgccttatgc
    13141 gatggcttta gaagacgagc tacctttcag taaacagctg acaaagcttt caaagaagtt
    13201 tacggttcct tatgttcctg cggttttcca gttagcagta gcttgtatta tgatgagcct
    13261 tggttctttc gactttttaa cagacatgtt gatttttgtg atgtggctat tcagtttact
    13321 gatttttatc ggtgtcttta ttttaagaaa aaaagcgccg gaattgccac gtccatataa
    13381 agtaccactt tatccaatcg ttccgattat tgcgattctc ggtgctattt tcattttagg
    13441 tatgacgatg atgacgcaaa cgaaattagc attgatcggt ataggtgtta ccttgatagg
    13501 tatcccagtc tattatcaaa aaaagaaaaa acgctcagaa gagtaaaata tttcaacaac
    13561 agacagaaaa agtacgaact aggtcctcta gatcttcgtg ctttttctat ttcaataaaa
    13621 caagttcttc tattttgatt tttgctactt ctgcaagtag tgcttatgtg ctaaagtaag
    13681 aatgaaaagg agagataaac atgatatttg attctcatac ccatttaaat gctgaacagt
    13741 tcaatgaaga tattccagaa acgattcagc gagcacaaga aatgggggta acgaaaatgg
    13801 cggtcgtggg cttcgatacg ccaacgattg aaaagtcttt gcaattaagc catgactatc
    13861 caaatatata cagtattatc ggctggcatc cgacagaggc tggcagttat acgaaagaaa
    13921 tcgaaaataa attacaagaa caattgactt taccaaaggt tgtagcatta ggggaaattg
    13981 gcttggatta tcattggatg gaagacccga aagaagtcca agataaagta tttcgcagac
    14041 agattgctat cgcaaaagaa atgaacctac caatcagtat tcatactaga gaagcacttg
    14101 aagacacgta tcaaatccta aaagaagaag atgttcgaga tatcggtgga atcatgcata
    14161 gtttcagtgg tgattatgaa tgggcaaaac gatttcttga cttagggatg catatttcat
    14221 ttagcggtgt cgtcactttc aaaaaagcat tagacgtcca agaggctgca gcaaatgtac
    14281 caatcgaacg attacttgtg gaaacagatg ccccttatct ggcaccagtt ccttttagag
    14341 gaaagcgaaa tgaaccaggc tatactcgtt atacagtaga aaagattgct gaacttcgga
    14401 atcttccgac agaagaagtg gctgcccaaa catggacaaa tgcgcaccga ttatttggtc
    14461 taagcgaaca tggataagat gaagatccaa gagattatag tcgttgaagg aaaagacgat
    14521 acaagaaggc tgcgggaagt agttgatgca gatacgatag aaacaatagg gtctgcaatc
    14581 aatgaagaga tcctgatgca aattgaacat gcccaagaga ctcgtggagt gatcgttttt
    14641 accgatcctg atttttcagg agaaaaaata agaaaaatca ttatggaagc tgttcctcag
    14701 gcaaaacatg cttttttgcc gcgcacacaa gctaaaccaa agaaaaaggg aagccttggt
    14761 gtcgagcatg caagtgatgc tgctatttta gaagcattga aaaaggtcgt gacgccagac
    14821 atttcccaaa cggctgtttc tgaaatcact agacaagacc ttttagacta tggtttaata
    14881 gctggggcac aagcaaaaga aaaacgagaa aaactaggtg acgagttgcg catcggttac
    14941 acgaacggca aacaattgga aaaacgtttg aagatgtttc gtattacgcc gaaagaactc
    15001 gcccaagcaa tgaaaatagt ggaggaacaa catgagtgaa tatcgtgaaa ttgcgacacc
    15061 ctctagaaca aaagagattt tattgaaaca tggtttttca tttaaaaaaa gtttaggaca
    15121 aaattttctg actgaaccaa atattttaag gaaaatcgta gaaactgcgc aaatagatga
    15181 acatacgaac gtgatcgaag ttggaccagg aatcggtgca ttgacggaac aattagccaa
    15241 gcatgcaaaa caagtcgtag catttgagat cgatgatcgt ttgatccctg tattggctga
    15301 tacgatgcaa ccctatgaca atgtgactat tgtacatcaa gatattttaa agaccgattt
    15361 gtcaacagct gttagagaaa catttcatga ggagcttccg ttaaaagtcg tagcgaattt
    15421 accttactat attacgacac cgatcatgat gcattttctt gaatcagatt tggttgtgga
    15481 tgaactagtc gtcatgatgc aaaaagaagt agctgatcgt atctctgctg aaccaggaac
    15541 gaaagcttat ggttcattat cgattgctgt tcaatattat atggaagcca gtttagcatt
    15601 catcgtacca aaaacagtat ttgtccctca gccaaatgta gattcagcga ttttgaaact
    15661 gacaagaaga gatataccgg cagtcgaagt gacagacgaa aaagcatttt tccgattgac
    15721 aaaagcggca tttcagcaaa gaagaaaaac gctttggaat aacttgcagc attcgtatgg
    15781 caaagatgac cagacaaaag catggttagc aaaaagcttg gaaacagctg gaatcgatcc
    15841 aaaacgtcgt ggggagactt tgtcattaca agaattcgcc gcattaagca atgcgatgtc
    15901 agaaaatatg cagtaaaaaa agaggttgtg acagaagttg tgaaaaagct gtcctgcatc
    15961 aagtaataag aacagccaaa caaaaatagt tcctcatgtt tttcgtttgt ttgggcttat
    16021 taccgcagat gcaagctttt gaacaccgtt taagaaagag gttgtgggcc tgccgtttaa
    16081 ctctaagtca caaggaacaa tttcaaaaaa cagcttctca tgtttttgag ttagttggtc
    16141 ttattacttg atgcaagctt ttgaccacta ttatgccgaa caaggagatg tgacacaaat
    16201 tttgtcacat ctcctatttt tgtttcttga tcaaaaaatt cttatacttg atactcacac
    16261 tcattttgta tggatttaga aaagaatatg tgtcttgaca gttattagta agtgagttat
    16321 actaagtcga catttatgtg tcaagacata ttaggagaga gaaaaaatga gaaaaaattc
    16381 agttcgaaaa ctaacgattt ctgctttatt gattgggatg ggggtcatta tcccaatggt
    16441 catgcctaaa atcatgattc caccagcttc ctttactttg gcgtctcatg tgccattatt
    16501 tattgcgatg ttttttacac caggagttgc agtggctgtt gcgctaggta cgacatttgg
    16561 tttcttttta acaacgccgg ttatcatcgc attgcgagca ttgtcacatc ttgtgtttgc
    16621 gttgattggg gcttggtact tgcaaaagca tccgagtatc gttttaaaaa atggacagtt
    16681 tacttttgct aattggcgtt tccaagggtt caattttgtg atcggtgtga ttcattcttt
    16741 agcagaaatg ctagtagtca gcatctttta ctttattggt aatatgcctg aaacatatta
    16801 ttctcaagga tatttttata cagtattcat cttgatgggg attggtgggc ttatccacag
    16861 tttagttgat tataatattg cttattttat agcaggaaca ctcagcaaat attttgatat
    16921 ccccgtattt acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag ggtacgcgaa
    16981 gaaaacgaca tattagacat gatctctcaa aaaaaaaact ctcatctagt aatggataat
    17041 aaatcctcta agatgagagt tttttgtcct aaaaagagct gcaccaacat cttctggcac
    17101 agctctaaca gggggcaatt tatggaagtt tgatgacttg ttctgtttct ttatcaaaga
    17161 agtggccttt gctgatgttg aaggcaaggt cgactatttc gcctggttta tggaaatctc
    17221 ttgcatctac ttttgagatg aactcagtag aaccagtttt tgtgtaaagc atcgtttcag
    17281 ctccaagtaa ttcggaaacg acaacttctg atttcactac ggcttcaggt gaagcttcaa
    17341 gggctacttg ttcactgtgg atgtcttctg gacgaatacc gaagataact tcttttcctt
    17401 cataaccttt ttcaactaat actttgtttt ttccttcagg aatacgaagc ttaagcccat
    17461 gtccatctgt gataacacca tttttcaatg tcacattaaa gaagttcatg gcaggagatc
    17521 caataaagcc ggcaacgaac atattgacgg gtgtatcata aacttctttc ggtgtaccga
    17581 tttgttggat aaatccgtct ttcatgataa cgattcggtc agccatagtc attgcttctg
    17641 tctgatcgtg agtaacatag atggtcgttg tttcaagacg ctggtgaagt ttggcaatct
    17701 cagcacgcat ggctacacgt aatttagcat ctaaatttga taatggctca tccattaaga
    17761 agacttttgc atcccgaacg attgcccgtc ccaatgccac acgttggcgt tgtccaccag
    17821 ataaagcagc tggttttcgt ttcaagtatt ctgtcagacc taagatctct gctgcattgt
    17881 ccacacgttt tttgatgtct gctttatcat atttacgtaa tttcaagcca aaagccatat
    17941 tatcaaaaac agtcatatga ggatacaatg cgtagttttg gaaaaccatg gcaatatcac
    18001 ggtctttagg tgccacgtca ttcatgactt ttccgccgat atttagttct ccttcagaga
    18061 tatcttccag tcccgcgatc attcgcaaag tagttgactt tccacaaccg gaaggaccaa
    18121 caaaaacgat aaattcacgg tctttgattg atagattaaa atcagttaca gaataatttt
    18181 ctgcattgtc atatttttta tatacgtttt tcagagccat ttctaccata atacgttcca
    18241 cactttcttt tttagtttta ttaactattt aaagtataaa tgaaagcgtt aactaacttc
    18301 aatgtcatgt tgaacaagaa ttttttcttt tttttgtcaa tgtgctagtt ttaagcttta
    18361 ataaatatcc ataagaaata aaaactgata cactggagta aataagtatg ttaacaatat
    18421 caaaaggagt gtctcttatg aaaatcgctt tgatcgctca tgaccgaaaa aaacctatga
    18481 tggtgaaatt agcaacagcg tataaaacaa tattggaaaa acatgaactt tttgctaccg
    18541 ggacgactgg gcagaggatc acggaagcaa ccggcttgcc gatccatcgg tttaaatccg
    18601 gaccgttagg cggggatcag caaattggag ccatgatttc ggaggatcag ttagatttgg
    18661 tgatttttct tcgagacccg ctggctgcac agccccatga accagatgta acagcactta
    18721 tccgtttatc tgatgtctat gagattccgt tagctacgaa tatcggaaca gctgaggtgc
    18781 tactaagagg actggaagcc ggcttcctag attttcgaag aatcgtccat gaaatagaca
    18841 agcatccact ttcattttga tggataagcg tatggaaaaa taacgtttaa tgttcgccta
    18901 tatgtagcta taaaagctaa acaaactgat aatatgcgat ataatacgaa cgtattggat
    18961 aatataaaca ataatattag tttttcgtat ttgcagtaaa tgtgaaaata tgctatagtt
    19021 tctgtgggaa aagatcccaa aaataataca gagtagaaaa taaagcgtta agtgctaaga
    19081 ggatgggatg ttgcctcttg gacgaaggac gtaatgttcg cggttttatt ttcgcattcg
    19141 ctgcatgata cgatgtagca gctcttaaag gagatgctaa aaatgaagaa acaccggtta
    19201 gacgcacgta tgattgctat tatgggctta ttgattgcat tgatggtcac tttgtcccga
    19261 ctggtagcaa tcgagacccc atttattaaa atcagtgtca catttattcc acaagtgatc
    19321 atgggcattc tttttggtcc tttttggtca gggataggcg cagttttagc tgatttagta
    19381 ggaatggccc tcttctcgaa atctgcattc tttattggat ttacattgaa tgcatttatt
    19441 gaaggagcaa tctacggatt ctttttctat cgaaaagaga tcacttggaa aaatgccatt
    19501 ctggctacgc ttagtgtcac gctgatcatc aacttatttt tgacaccatt atggcttgcg
    19561 ttgatgtacc atgtgccatt gttcagctgg gtcgtttggg cgccccgcct gttaaaaaca
    19621 gtaatatggt taccgattca atcaattgcc atttattacg taggacgtag tattccttac
    19681 aaaaaaattt taagaagttt agcgatccac gctaaataaa aaacaagcag tcttcccatc
    19741 ccagaagact gcttgttttt attgtgctgt gaattgtgct tgatagagcc gtcggtaata
    19801 accatctttt tgaagcagct catcatgggt gcccatttcg acaatagctc cctgatccat
    19861 cactaatatc tggtccgcat ttttgattgt ggacaggcga tgggcaatca caaaactggt
    19921 ttttccttcc atcattgcta agaaggcttg ttggattttt ttctctgtca atgtatcgac
    19981 tgagctggta gcttcatcta agatcaacat agggggatta ctgatcatcg ttcgagcaat
    20041 cgtcaacagt tggcgttgtc catctgatat ctttaaccct tgaccgccaa tcttagtatc
    20101 taatttttcc ggcaaacgtt ctacaaattc atacatataa gaggctttta gggcatcatt
    20161 gatttctgcg tcacttgctt ccggattccc aaaacgaaga ttttctcgca atgtactgtc
    20221 aaatagccaa gtatcttgta acaccattcc aaaactttta cgtaatgtat cacgttggat
    20281 agagcggatg tcatggtcat cgactgtaat tgttcctttt gttgtgtcat aaaaacgcat
    20341 cagcaaatta acaagtgtgg attttccggc ccctgttttt cctactatag caatggtttg
    20401 acctggataa gcagtaaaat tgaaatcttc gatcaaacgc tgcttggggt cataagaaaa
    20461 agaaacatga tcaaaagaga cttttccttt gatatttttg ggttccaagg cataggcatc
    20521 tggcaaatca ggcttttctt ctggctgatc gatcaaatca aatgctcgtt ctagaccaga
    20581 aaaagcggtt tgcagttgtg tcgtgattcc agaaagttcg ataaatggtt tagaaaactg
    20641 acttgagtag atcgtaaagc tggaaatcac accaacagtg attgacgaac cactcttcaa
    20701 tgccaatagg ccgccaacta aaccaatcga taaataagca aggtgatcta caaaacgaga
    20761 agacgggttt gtcaaggaag aagaaaattg tgcccgttgt cctttttcat atagatcagc
    20821 atttagcagt tcaaattgct gttgactagc ttcttcttgc tgaaaagctt tcacgatttt
    20881 ctggttaccg attcgttcgg tcacaaagcc cgagatatcc ccaataatcc tttgctgtct
    20941 atcgaaattt gtttgtgaag cacgagcaac gatccaactc acaaggaaaa taattggtgt
    21001 agaacaaaga acgaccaatg tcagaagtgg acttagacgc atcataaaaa taaacgaaag
    21061 aatgatgatt gccactccag aaaataattg attgaaggcc gcagaaattg caatggaaat
    21121 attgtccata tcatttgtaa atcgactgac gatatttccg tgggagtttt gatcataata
    21181 acgaagtggc aatcgattca gatgagcaaa tgcatctttt cttaattttg caacggataa
    21241 gtaagctaac cgattgctta acacttggat gagccattga ctgactacgt tgattaaaac
    21301 gataccaccg aatagaataa gtaaatggaa cagtcgattg aagcggacct gtccttctcc
    21361 aatcatttga tcgatacttt gaccaatttg taacgtcatc acaactgttg caatcccatt
    21421 gatgattcct agtatcacag ctagccaaat gtcttttgca taggcagtaa ggtaaggaat
    21481 gaatcggcgg agcgtctgca aggataattt ttttactttc ataactattc ttcctcctgt
    21541 gatgcgacga tttcctgata ttctgctgat tgagcaagta attcttcatg ggtaccctga
    21601 gcagcaatct taccttgatc catcactaag atatgatgtg cttcttgaat agagcggaca
    21661 cgttgggaga taataatcac tgtactgcca agataagtct gtagcgcctt acgcaaatcc
    21721 aagtctgttt gataatccaa cgcacttaaa gagtcatcta aaatcagtaa tgctggcttg
    21781 gcaatcaaag ctcttgcaat cgtcagtcgc tgtttttgtc cgccagagaa atttttccca
    21841 ttttccatga cttttgtatc taaaccattt ggaagctgac ggacaaattc ttctgcctga
    21901 gcgattttca gagcttccca acattctttt tctgtggcgt ctttcttacc ccattgaagg
    21961 ttttcacgaa tagtcccggt aaatagtaca gaagtttggg gaacaagagc aatttgctga
    22021 cggagatgat ccagctgcca agaacgaaca tccattccgt ccattataac tgtaccggaa
    22081 ctgacatcat agaaacgcgg aatcaattgt gtcaaagtcg ttttcccgct tccggttgga
    22141 ccaatgattc caaggactgt atttcgcttc agctgaaatg taatattttg gagagaaaga
    22201 ccagaatcag gagtataacg gaagtcaaca tggtcaaaag cgataattgc ttcagaagaa
    22261 aaatcgggag tagtactgac ctcagcttca agaatcgaat tttctgtgtc tagcacctct
    22321 ttcacccgat ttcctgaagc ttcggcacgt gtgaagatca cgacgaggtt tgaaacaacg
    22381 atcagtgcca aaagcatttg attcatataa ttgatcaaag caagaacttg tccttgttgc
    22441 aagctaccaa tatttacttt gatcccgcca actgtcagca aacagatgat cccgacattc
    22501 atgatcaagg ttgtagcagg tgataatagg gcagagatat tggaaacacg taaataattg
    22561 ttcgctagat catttgtgct tttatcgaac ttctctgttt ctttttttgt ctgcgcaaat
    22621 gcgcggatga cacgaacacc gctaagattc tgactaacgt gttcattgag ctgatctagt
    22681 ttttcttgca ctttttggta gagaggaacg gtttttttga tgataaaaaa taaaatgatt
    22741 gagaaaatag gcaataatgc taagaaaaac agacctactt cccagtcgat cacaaaagcc
    22801 atgaccactg acccgatact taaaaacggt gctctgatca ataaacgaat gaccattgct
    22861 aaagctagct gaagctgatt gatgtcgttt gtcattcggg tgatcaaggt atctgttcca
    22921 aaatgattca actcgttgtg agagaaagta ttgatttttt tgattagctg attacgtaac
    22981 tctgtaccaa atccttgtga agctactgaa gcatagtatt ggcaaatgat agcacaaatc
    23041 agaccaatca cagacatagc gaccatccat aagcccattt caatgatttt tcctcggtca
    23101 ttcattttta gtccattatc gatcaaggaa gccatcatta aaggcaggac aagttcgaag
    23161 acagcttcaa gaaatttgaa gaacgggccc aaaatgattt gcttacgata gtcttttgca
    23221 taacgtaata actgaatcat atgattcctc ctattattta tttgtccaca attgaaaaaa
    23281 caagtactac cacattgtag cttaggaaat ttagaatgaa aagagaacaa gccttttcat
    23341 aaatgaaagt tttattatga agaagcttta gaaattgaat agactatttt ttttaaaaaa
    23401 aagaaaatga gaaaaca
//
LOCUS       DAYYAO010000045        23040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000045 DAYYAO010000000
VERSION     DAYYAO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.047"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2569)
                     /locus_tag="ACHARI_001645"
     CDS             complement(<1..2569)
                     /locus_tag="ACHARI_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8442695.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
     gene            complement(2791..3675)
                     /locus_tag="ACHARI_001646"
     CDS             complement(2791..3675)
                     /locus_tag="ACHARI_001646"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8442696.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3770..4207)
                     /locus_tag="ACHARI_001647"
     CDS             complement(3770..4207)
                     /locus_tag="ACHARI_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442697.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4424..5335)
                     /locus_tag="ACHARI_001648"
     CDS             complement(4424..5335)
                     /locus_tag="ACHARI_001648"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8442698.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5712..6350)
                     /locus_tag="ACHARI_001649"
     CDS             complement(5712..6350)
                     /locus_tag="ACHARI_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442699.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6354..8336)
                     /locus_tag="ACHARI_001650"
                     /pseudo
     CDS             complement(6354..8336)
                     /locus_tag="ACHARI_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            8516..9181
                     /locus_tag="ACHARI_001651"
     CDS             8516..9181
                     /locus_tag="ACHARI_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8442700.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="ACHARI_001652"
     CDS             complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="ACHARI_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFM8442701.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="ACHARI_001653"
     CDS             complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="ACHARI_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFM8442702.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="ACHARI_001654"
     CDS             complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="ACHARI_001654"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFM8442703.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="ACHARI_001655"
     CDS             complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="ACHARI_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFM8442704.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21759..22334)
                     /locus_tag="ACHARI_001656"
     CDS             complement(21759..22334)
                     /locus_tag="ACHARI_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFM8442705.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22334..22933)
                     /locus_tag="ACHARI_001657"
     CDS             complement(22334..22933)
                     /locus_tag="ACHARI_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFM8442706.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4532 c         3344 g         8171 t
ORIGIN      
        1 tttctgggat agaaagaaca aatttacctg tatcatcaat agttgtttcg gacaaaacag
       61 cttgattttt tttcaatgaa accaaagctc ctgcttcgcc tgtacctgtg attgagcgag
      121 aagctgcatt gatcaatgtg gtacttaatt tagcaggctc aggaggaatc acatcgactg
      181 tcgttttggc agagtccagt tctgtcactt cttggccgcc tcttactttt ttagcagcaa
      241 ctatttgcac ttgatccccc gttgttagtc tttcttcgtt gggtacttga acctcaaaca
      301 aaccgccttg ttcgccttct ccccacggat tatatcctgt cccttcattt tcatctgttt
      361 gtccttgtgt gagagcagta atttctacag gtgatttcgt cgtatctgca taagtaatac
      421 gtaaagtcac ttctacttcg cctgtccaag catctcttgg ctttgcctcg acaccttcgg
      481 gaacaacgac atgcccatag atttttttat ctgcattcgt tggtactctt aaggattcaa
      541 tcatcggaag ctggttattg gcactgattc gattataatt tttcattccg gcacctgtct
      601 gagcaaattc atttgttaaa cctgcactag ggcttgatac aaaattacct aagtcggttc
      661 cattgatcga taagtctgtc atatcagaaa aataatccgg atctttttca aagttatcga
      721 acgttactcc tgcctttcgc cataaagcaa tttctgaatg agcgatcttc aaagaagaat
      781 cgccatttcc ttgaaatacc cagccaccta tgccattagt ctcgcttccg atccgtttgt
      841 tttggaaatc gaaataggta ggagattcta agtctacatt caacacatcc ccactgatga
      901 tgccagtctt atcttcccca gttttcccga ccatgactaa ataagttttt tcgccagcca
      961 taaagttcaa atcaatatct ccagttgtat ctacggtagc tccggaatct gcgcgtatat
     1021 cgatatttga tttttctcca gtaaccgtaa aatcagctga ccctggactt tctagaaaac
     1081 cagaagcgtc ataaaattgg atcgcttggt ttccttgatt gagtctgttc acataagatt
     1141 tgccatcccc tgcatttctt accttcaatg agcctccatt agttactgtt agtttttgcg
     1201 taccgttctc aaaacgaact gcgctagaac gaccactttg cttatcaata tcgatgactg
     1261 atccggctcc ttctacgatc aaagaaagtc ctttccctac aaaacgcatc ccattgtttc
     1321 caggttggtc gccatctcca gccattttga ccgagatttg tccaccactt gatacatgga
     1381 tttgactaga atctccttgc aggcgaaatc cgccagtagt cgctgtattg attgtgattt
     1441 tcccatcatt cgacacagcg actcttgatc ctgagcctct catttggata attccgcgat
     1501 tgttgtcgtt ggtaatattg atctttgtat ttaccgaaaa ttcacttcct gagccactga
     1561 ctttaaatgt gacattcccg ggtagcgtat cgacagcctc tccttcatcg ctgacgccat
     1621 ctgcccgatc atagtcaaga gagacctttg aaccactcaa gatatggagt cctggtgctg
     1681 tttttccact gcccgtctca tcacctgaat ctagaaaacc atagaatttt cctagatatg
     1741 gtccataaag ctgactacca ttgattacat ttagctgata gcttctacaa aaataagctt
     1801 tacccggagc ggcattcatc ccttctttca ctggttggac atccaataca ccggaaacac
     1861 catctaatgt gacagttgca cgaggggcgt aaatcaggcc taatttagaa ccatcatata
     1921 agtcaagtgt cccggtaaat tttatttctc ccgtaaaatg atatctgttt ccccaaaaat
     1981 cggacatata agcaatgata atggcatttc catcgatatt tggtatcaac ccacctggat
     2041 gagctgccgt tcctttaaaa ttctgaaact cgatcgactg aggtgtctca ttgatataga
     2101 tgtgatgtgt gccgaaatta tgagcaagtc cgccaaaatc gataacgata tctcgcttga
     2161 tctccaattc cctgtctaaa gtcaaaggct gagttaaacg aatcttagct actgattcgt
     2221 ctgcgagaag ctgcgtcagt tcttctgttg tggcagcttc gctgtaccca tccgctgaaa
     2281 caactctaac ctgtacctct tcactgctat cagacgtttg ttcagctact tctgaggtct
     2341 cacttgtctt attttcttct tgccttgtat tagaagtagg cgcctcactt tgggattctg
     2401 tagtttcaga agtaattgat tcttctgaaa aagtagtctt gtcaaaactt tgattctctg
     2461 attgcgttga ttcgcttgtt ttctctacat cggcaaaaac ggatgtcacc atcaagagag
     2521 ggaagacaaa caaaatcgac gcaatcagct tttttacata ttgattcatt ctccaccatc
     2581 ctctctgacg ttttgacaat tttattttaa cgttttaatt tatttgtaat tgtactttca
     2641 tatttaaaat atgtattttt atggcaaatt tttctgttag gtgaaaattt cttttttgta
     2701 caaaaaaagg ctgtgacaaa agtcttgtca cagtccctta ttccgaacaa ccgatgtcct
     2761 cctgacaact cttcggtcac aacctccatt ttaacaataa acctgtaaat gtgagggtaa
     2821 cactttgatt gtcacaggta atttatcccc ttcatctcca tcaacattcg tcgtcaattc
     2881 ttcctcatca gctaatgaca caactaattg ctcacaagta agataggcta gatttttcgt
     2941 agattcatct actcctttta ataggtctgg caatgtttta agcgtatcga tcaaagaaga
     3001 atcttttaaa taaaccaaat ggagcttacc atcatccact tttgcatttg gaagtaatgt
     3061 ttcaaaaccg cctactgagt tagttagtcc tatcagcaag atactgctct caatttcttc
     3121 cttctttcca tcaacttcta cgtgaaacga gtaggattgt gtggaagcta actgtttgat
     3181 tcctgacatg aaataagcta gttttccaaa tttcgtcttc ttctccgcat ctacatcatt
     3241 gattgcttca gggatagagc caattgcgac gacattcata aaatagtcgt catttatttt
     3301 tccaatatcc aatggcttga ctgattcaat cgaaaaatga ttgatcgctt cctcaggttc
     3361 tagcggtatc cctaatgctc gagccaagtc attcaccgta cccaatggga aaaatccgaa
     3421 atttggccga tgttcttgtt cagcgattcc actgattcct tcgttgactg ttccatctcc
     3481 gcccatgaca aacacactat gatacccttc cgtagccgct tcgcgagtaa agttttttgc
     3541 atcccctgct ttttttgtat gcaagacgac cacttcatca aacaccgatt ccaattttgc
     3601 tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct gaactggggt ttaccactaa
     3661 taaaactttt ttcatcactt tctttccctt ctgattgatt tctttaatat aacgatagca
     3721 taaagaaaaa aattaggctg tgatgaagta ctgttcatga aacattcatt tattttttga
     3781 caagataaaa tacccatact atgaaactga ggaaaattaa aacaatgata taggaaataa
     3841 aagaaaatga taggcattgt atacctaata aagtaaaaat cttaggtggc agataaaaac
     3901 acaaaaatag caagagaatg taatagcgtt tcttccactt tttgttatca gaccttatag
     3961 cgatcagagg aataccaaat gcataactga aaaactcttg atgtactgtt aatatgtcac
     4021 gaccttgaat aataaatgca acaatccccg ctataattag tgtgcctaat accaaaacgg
     4081 aaaattgatg aatatatttt atatacaaat gatccattgt acttcctgcc tttttatttg
     4141 tgagaaaaac aaatccgtat acgtgaataa cttctagaaa taaaagaaat ttgcaaaacc
     4201 actccattta tctacttcta cgtgcatttt ttaatatcgt tttctttatt taagattata
     4261 cagaaaaata tctagggtgg ctggattcta agtaaaaaaa atttttaaaa taaaaagaac
     4321 taaatcaagt aataattaaa ctactgttga tttagttcta ataaaaagat ttagatgaac
     4381 agttttattg atttaaatag ttcatatttt ttatttatac cgcttattta ctacttgctg
     4441 tactagaatc tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta
     4501 cattagcgtc tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt
     4561 ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt
     4621 tttggttttt caccatttta accacataat attccgtagt ataagttgaa gcattggtag
     4681 aagtgatcac atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg
     4741 ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag
     4801 tatcagttga cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct
     4861 tcacatcttt tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat
     4921 ggaaagattc ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac
     4981 cttcttgaaa ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg
     5041 cagatttcaa ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattggg
     5101 aatcgatttc tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga
     5161 tcaactgttg cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt
     5221 gttcgctgat tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc
     5281 ctgacaataa gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc
     5341 aattctccta cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat
     5401 catcaagaac acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa
     5461 tccagaaagt tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac
     5521 ttcttttgtt accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt
     5581 agaacaaatg ttatcgattt tgtttttctt cttccgctgt tgggatattc aaatcagaca
     5641 cttttatgtt cgattttcca gacatttttt tgttgttttt attagaaaaa gtttgttttt
     5701 tcatttgttt tctaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc
     5761 gtgaccacga taactaacgt accttgggaa gcctgttgcg ataagatttc aacgacttgc
     5821 tccttatttc gtttatctaa tgatccagtt ggttcatcgg ctaaaatgat tttgctattc
     5881 ttcaataaga ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata
     5941 ggcgtactta gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc
     6001 ttatctgaaa catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt
     6061 tcatattcaa tcaacccaaa atcttgaaaa agaaaagaga gggcatttct ttttatcaag
     6121 cgcttctctc ttccagttaa atgttttgtt tcttgattaa ataaataaaa ttgccctgaa
     6181 ctgaattgat cattcaatcc taatatattc aataaagtac ttttccctgc tccgctctct
     6241 ccaactacag tcacaaaatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt
     6301 ttgtttcgaa tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct
     6361 ccattcatca gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga
     6421 ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata
     6481 agaaaaagga agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct
     6541 ttatacgttt caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg
     6601 tagacaaaat atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt
     6661 aaaaatatct gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca
     6721 gtctgagtat acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc
     6781 tcaaataacc tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct
     6841 ccgctgagaa tatttctttc ccaatacgta ctgtttttca atgtcagtat ttcaacaacc
     6901 ggataatgtt cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt
     6961 ataaatagaa atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc
     7021 aggtcatttt ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt
     7081 tctttttcgg ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga
     7141 taattcagat ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct
     7201 gatacattga ctaatatact tccgttttta ttcaaagttt catataattc ctgatcggct
     7261 gctgatgcaa aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga
     7321 taaaatacat gatagtcctc accagcgatt tcttttggtg tttcatggga aacagctaac
     7381 tgtgaagaag aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa
     7441 aacaacaatc caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa
     7501 gcgatttttt caactaataa aagagaaatc aaagagatac cctatattcc gctgaacaag
     7561 aaaacgtaca tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca
     7621 ccaatgattc ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac
     7681 aatacttgat gtaatgaaaa gccattattc aacaataaac tgatttcttt attcatcaat
     7741 aagaaaaagg atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga
     7801 taattatata cgtctgtact tgaaataacc gaatacgaat agctattttc aaagtcttca
     7861 aaaatattag tggcataatc agtgtgaaat gcttgattga atcgttccac aaatagtaaa
     7921 atgaaccgtt cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct
     7981 tttattttga tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc
     8041 ttctcgtgag aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt
     8101 atcttgaagt tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa
     8161 gaaagctcag tttctgatga gacattttca aataccatga ttagctgctc atattcttct
     8221 tgcccaataa aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat
     8281 tcatttgtcc gatagagtac ctgtttattt ataataacca aggcaaaacc actcatgata
     8341 ccggtcacta tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct
     8401 tacttaaatg attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa
     8461 atgtataggt catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa
     8521 catcggagaa aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg
     8581 cgaaaaaatc ggtgtttccg ataaaacaat ctctagttgg gaaaattctc gtacaatgcc
     8641 agaccttgaa atgctgtcac ttttacatca gactttaggt ctgccgattg attttccttc
     8701 tattgaatca gctaccgaat caatagaacc aacattgccg gatgaacctc ttgttatcaa
     8761 gaaatcttat tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct
     8821 cgtcttttgt ttggtctatc tttttcttat caattatcaa tataaaaatg aaacggttga
     8881 tcgattcaat ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt
     8941 ggaaaattcc atcgaacatt gggtatgtaa tgatatcttt gttcatggta catggctgtc
     9001 ttttcacgct ggagaggtag aaaaaggtaa gaatcatgct gtaatcaaac acaaaggaaa
     9061 acacgtcatg aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat
     9121 cctcatccaa gactatgacg atgccttaaa aagaagccct caggagccag ataggcatta
     9181 agtaggagat gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc aaaaaaaaac
     9241 aggggtacaa attaattgaa aaagctttga gatgaaagtt cctaaataaa aaagttcaga
     9301 atgataggta aaaaaatgac taactattct agtattaggc tatttgtatt attcttctgt
     9361 tatttttata tcaaaaaatc cagaaataac taaatatagg tatttctggc ttatagtata
     9421 atatttacgc cacagccccc aatgattctt tttggaatca tgatcaagga cggacataca
     9481 caaaaatatt ttgcccattc gatccgaata gattaacgac taatctagta agtaatatag
     9541 attggtacgt agtcaaacca gcaaatggaa aagttgtcta ttccacccca agggctttaa
     9601 agacagcatc ttccggcgtt tgataaaaaa tcaaattaaa ggtacccatt aattctgggg
     9661 gtacagttcc aatatcagca gcgcttatca taggtacaag tacttttttc gctccggcat
     9721 cttttgctac ttgtaaacag ttagctaaat tatctacctt aatcatagaa ccaccaattg
     9781 taatatcccc taaaataact aaacttggta atacaggttt atttaaggaa actgagcata
     9841 aagctattat agttgataac gataatgttt ttgtcatacc aatgccattc atatcttgaa
     9901 catgaacgac aaaatctttt gtcgtcaaac taatagactg actaacactt ccataattag
     9961 ctcttaaata tcggtatgcc gaatccatcg cttctcttgc tccagcatct gaattgatcc
    10021 cagtacgttc aaactttcca tttcccgaag tcatttgcgt ttccaattta taggtaccta
    10081 attgcccagt tttgccataa gataccgtgt acaaatgccc tggcttccct aacccttctg
    10141 gaatcaaagt tcctccacct tgttcaggaa cagaaacata ctcttctcgc tcttcttcta
    10201 aatcaatgta tgaaaaattg acatcataaa attccattcc accaattttc tttaattgtt
    10261 cttttacacg acgacgcatt tcaagagcaa atcttagtat ttcttcgaca tcactttttt
    10321 caaatctacc atcaggatac aacagtttca tcatcccaga tacaatccga cgaactgcaa
    10381 tcgtatcccg ctgatttaat tgagaaccaa gtttgaaata tttatcaaaa ctatcgccaa
    10441 aactaacttt tcgaagctca cgcatgactt cagaaaaata atctgtaatg aagccatagt
    10501 catttgtaaa aaaatctggt cgatatttag gaatttccca acctggtaaa taacaatgca
    10561 tccgatccaa aaaggctgaa tccgtcccca taacctccgg aaatggctca aataaattcg
    10621 aagttttcaa tattacatca acgctttgat taatatttcc tacaaaagcc attgatgctg
    10681 aagcattctt ttcttctttg cctctagcaa aagatcctga agccatgtaa tctttcatga
    10741 tttgaacgcc atcattatcc ttaaatttaa tcccagcaac ttcgtcaaaa gcaacacagt
    10801 cccacatacc tacaagtccc attgtacgac tagccatatt atagaaaagg tttgctactg
    10861 ttgtttgtcc acctgaaaca aggattgagt ttggtgaaat ttctttatat acatgcgatt
    10921 tacctgtacc acgtggtcct agttcacaga gattaaaatt attttcaatt aatggaataa
    10981 gtcttgctaa atgaagccat tttacccttt tattaaattg tgtaggttcc ataccaatgg
    11041 accgtagaag taagtcaatc cattcatttg tagtaaactc cttgcgagcc atcttgtaag
    11101 cctcaatgtc taaaccaggc atttggattg gatcaacctt ttttgcaata aaagggctat
    11161 tccaacgatc attctcatca taccaatatt ccatcgaaac aatacaccag atccccccac
    11221 ctaataatcg atcaaatttt ttcggatact cttcactaat ttgaacattt tttaatccca
    11281 ggttagagaa ttctgcttca taacaatctt tcttttcatt taaatgtaca gttactttat
    11341 caataactgt ataaccaccc aattcacgaa tttttgcttt tactttttct gcttcatcag
    11401 gacgcacata gtttcgagca agaatatttt taacattttc cacacctttt tcaaccaatg
    11461 tttcatccaa tgacgaagca tacataccaa gtaaatattc caatacataa acaggtacat
    11521 tcgcaccttc tttaattttc tttgttaaat cttttctgac aatctttccg ggaaagaatt
    11581 cagataactt taaaatttcc tgttgttcca ctagaattcc tccatgtgtt cagtcattat
    11641 aatagcaatt aaagtatatc atacttaacc agtaggcttg ataatacaaa agaaaaagta
    11701 tccacaaaaa attgtggata cctcgttttt atatatcaaa atcaaatgat tctgcaaaag
    11761 gattatctat aatgaatttt attcgctcgg tcaaatcatt tttttgattt tttatcacta
    11821 aataataagt atcttcaact gtatatcgac gatttacaaa ttcaaaaaca aatttagtaa
    11881 accgctgatt tgcatcttct gtttttgaat ccgcaacata gatttcttca tttgagatac
    11941 gttcaccata ttcatcttca aaataaagcc agaaagatgc ggggtcataa acatcactaa
    12001 ttgcattttt ttgatagaat tctaacgtgg ttgataatcc tgtaattctg cggttgctag
    12061 tcattaattc aattgctact ggttcctttg cattgctacc tttggctacc gtaatttcta
    12121 gaacagggac tagcatttct tgaatagatg accctccatg aacgtagctt tgtcccccgc
    12181 cagctttctt aaaaatactt gaagtaatcg ggacacaaat gtttctagtg tcatcatttc
    12241 ctaaagaatc tcccaaagag taatttttcg taccaattat ttcgtattct tcttcactaa
    12301 gaataaaacg acgttctaca cgatcttttt catttctaga tggattttca atcttatcta
    12361 cttcttcaac gtggcttctt ttataaataa atccatgatc agccgtaact ataaaacgat
    12421 agatactgcc atcattatgc aagcgattaa taagtcggga aatttcgctt atcgctaaat
    12481 cacaggcctc aaaaactcga ttttcattcg agtgatcacc tgttctatcc acttgatcgt
    12541 gataaatata aatgactgat ttgccattaa atacttctcg tacctcagcc ttattcatct
    12601 taaccacatc atcataattt aaagctacag catttgcatc tcgttgctgg agaatcgcat
    12661 ttcgattgga taaaccattc gtttttttcc catcaatcag tacttctgtt ccttcattgt
    12721 aagttagttg attgtttggt aataaggcgg ctttaccgaa ttcagtaact gaaggtaata
    12781 cagaaagaaa gctagccatg tttgttgaat acttcttttc tctaactaac ttacgttcta
    12841 aattcatccc taactcatat cgaaaagcat ctgaaataat aactacagtt ttaacagagc
    12901 gtttcattac agcttgatta taaaattttc gaatggatgg acgattctta aattcaaaag
    12961 tttgattcca aatttgtcct gattgatcaa gaaacttcga ataatttgct tcaactaatc
    13021 gatacaattg gccatatttt tgcttttctt ctatatgatc actattccaa ataaatttcc
    13081 gataatgcgt atcaacccaa tacatttcct tctcatattg agttagcaaa ttatccatgt
    13141 tcatcggcaa gcacaatctg tttttatcca caagataata agcatgtttc aataattgat
    13201 actcataaat ataatccgat gcgaaatgca ttttttcgcg ctgctttaaa ctatcaaaaa
    13261 ttgtttgtcc tgcaattttc gttgtagtgt cagaagcctt taatcgttct gcataaaaca
    13321 ttaatatttt tgcatgaatc tcagaaaata cgtctgcttc aattagatca tcaatttcta
    13381 tactgtctaa taatccatca ccatcaatag cctgatatac ttttttagat agctgttcaa
    13441 agctttccat gtaacgtgta tcattcataa actgatccat gaaagtaata atgtttgctt
    13501 tcaaatgtag tatttcaaat cctttgaact tcttcggcat ctcttgatca atttgataat
    13561 aaaaatagtt caaataaaga cacatcgtca gcttttccaa tgttggctgg ctatccgtaa
    13621 agccaaattt catttgaata aattcccaaa atttagctgt taactgatac ttatcaagtt
    13681 cccttaaagg attctcccta ctttctgtaa agctttttag aactacacgc agtaaatcat
    13741 ttaatcgaat acttttcgat ttcaacaaaa cagccattat cattaattca atcgtttctg
    13801 atttattaat tgtcatatgc gtactaaatt caccaaaact tctcatgcga tcctttgcat
    13861 taaagaaatt acgatactgt ttaactgtct gatgaaatct aagtggaata gccaattcat
    13921 tcatgataat cgctaagcgg tctgcactaa attcctctga ataattcaat acactcaaca
    13981 aatgattatc ttcatcttta tcatcccaac gaggaaatgg cgcatagatt aaatagttag
    14041 tttcgggatc ttcatattcc aataaacgct tagttttaaa ttgtgcgtcc ttttctaatc
    14101 gatagatttt tgcattttga atgaccaatg agtcaatttc ttcaagaaaa tcctgatttg
    14161 aatcaaacca aaaaatgatt ttcctacatc cataattagg aaaacgttga tttaattttt
    14221 cttcaatttg ttgcgtattt atatctgtca tcaatctcac ctaatcgttc tctttctata
    14281 ccccttatat tgacataatt tggataattt cgcacgcaat ttaagaataa aaaagaaagt
    14341 tgttagaaaa caactttctt agataactat ttttttggtt ctttgacccc ttttgcaaaa
    14401 atttggaatt tctcgccggt tttaggatca cgctgcaact tatcataatt gacaattaca
    14461 ccatcatcta agtctaattc aattcgtttt aatgctaagt gacccaactt ctcgtcatac
    14521 tcacgaactt cttttaattg cttctgaatc tttacaattt cttttgtcat tgcatttctt
    14581 tcttttgggt tatcacttgt tgaaatttgt tgctgacgca attcgattcg attgtcatac
    14641 gctttttgta gttcgtgtag gtattcagta cgtacttttc ctaattgatc tggtgtatag
    14701 cgatgcaagt acattaacgc tttaaagcca ttttgttttc ctgagtccaa ttgccaataa
    14761 attggtcgat tggagtatgt ttttaaatgg tctttgtaaa aatcttgtac gaagtaacga
    14821 cgaataattt ctcgacttgt ccctttgcca cctaaagtgt cagcgataaa ctggagattt
    14881 tcttcaaggg tttctttacc aaaagtaatc tcaacgaaat caatgaaacg attgacaatg
    14941 tcaatttggc tatccttgaa atactcatca tcggtgatga gtaaaacatt atcagtatca
    15001 ggtttaaatt tttgatagcg ggaaggatcc cattcgccgc cagcgaagat gagaccatct
    15061 tcgtctaggg agtaacgacc gaagatacaa cctattgaat aagaaataaa atccttaatt
    15121 tccctcgcct tatcaggtaa ccttgcagag atatattcat cttgaacctc ttttgttaat
    15181 tctttttcaa gagaataatg attaatgaat atgtcgttca gttcctcttc attcgatttt
    15241 agagtattaa attgattaac caactgccta cttagacagt taaatgcctc tgagagatct
    15301 tttttaaaca tataacttaa caaaatgttt cttctaaact cgtatgacgt ttcaagagca
    15361 ttatagtctt ttctagctat atcgatattt tcatccactt tttctcttat tttttttaat
    15421 tttctctcag atgtaacttt aagaaaaggt agttttccca ttggtccttc atggtaatcc
    15481 aaggtgggag aaataaaagt taaaaagtga ttaactaatt tagagtttac atagccaaat
    15541 atttcttcaa agtcagttat atcctttgag aacagaacag atcctttagt ttcaaaaatt
    15601 gcctgattct gcaatctaaa agatacttgt ccagatgaaa gtgtcgacca agtaattccc
    15661 tttttaaaat agtaattaag attttgtggt ctagatttta acttcccatt ttcattaaca
    15721 taatttttta tttcatctcc atcattctcc cagttaacca cgtattcatt gtttccatac
    15781 cattttctaa aacgaccacc tttagttgta ggaaaccatt tttttctaga ctttttagcg
    15841 tcctctaatg ttttagcatc aaactttatt ttgttaaatt ctacttcaaa ccaataccgc
    15901 aaaaatttat tgttatttcc ggttgccaac ccttgttttg catctgcaac atcgttagtt
    15961 aattttcctt gagcaaaatc agtgattacg ttatcgcttg cccagtaagc aatcggcatt
    16021 cctgggattt ttttgaagtt gtcttgatta gtactataaa aataatcgca ttcaccatgc
    16081 tgatgaattg cttctaacgt tttaattttt tgaacttcca ttccaccttt aaaatctgat
    16141 aattttatat agcttccatc ttgttcattt gaatttttta tcgtaaacgt acaaattgga
    16201 acggttgctt cttcaaaagc tgaatactcc atttgaatca aactagaaat gtttctttca
    16261 ttaattaaaa atgagcgtaa cttttcatag ctagaaatga acatccaaac aaatggcgtc
    16321 ataaaggaag catatccatt atcaatcagc atttggctat tcaatttaat aaacacagaa
    16381 aatagatctt ttttgacatc agaatagtta tcatttacat atttttttaa tttcttatcc
    16441 attttattaa gataaggcgg attagtgact accgctacat atttatcaac tagcatttca
    16501 gccacgtcaa gaatgtgcaa gtatttttct tgtatttcct tcatcccata aagaatatcc
    16561 atattcgtaa attcgttgaa ttttgatata tactctctta cttttcctaa gtattcttta
    16621 gttattgcct gattattttt atcgactaac ttaatgattg atccaagttc tttcccattt
    16681 tcaaattgat caaaaattaa ctctttgtat tcattccagc gttcactata cttatcattg
    16741 ttcaattcac taatacgctc ttgatactcg tctgtcactt taggagaatc aataaactct
    16801 cttaaattcg gcgatagact ttttttcaaa aaaagacgat cgtattgacg ccctttcatc
    16861 ataattgcaa aataagacaa ttgatacgct cttttatcga tttcaagacc atataaattg
    16921 tattcaacga ttgcacgtgc agcatcacgc tcagaatatc ctaacgtaag ataaatttcc
    16981 atcaatacat cgaaggcgta aactaaaata tgcccagacc ccattgcgtt atcgataatt
    17041 tttagttctt ctggttttaa attacttaca gatgttgctt gttcaaaagc atatttatct
    17101 ggaattaagt atttcaactt atttcttaat tcactattgg gataatgatc taaccaatac
    17161 tttcctaaac tattgtcaac catataacga acgacccaat ctgtggtaaa tagttgagtt
    17221 gctgccggaa tatcttgttt tttgacaatc cctttataaa tattcaccac ttgattatgt
    17281 ggctcactat tatagtactg atacaaccac ccaataatct ctacctgtcc accagcttga
    17341 acatagaaat catcctctgg aatacttaaa agatcagcga ttacaccatc ttttttaata
    17401 taagaagctg taaacaaaag ttctgtataa tcttctactt cttcaaatag ctcaggtaag
    17461 taatcattca attgattaca gatttttata aaaatcaatt gatacagctt gtccattgct
    17521 tctgctgaat tatctgtttt ccaagttctg acttgttgct gttcattact agataattct
    17581 tcaaagatat cggcatcata aatatctgta ataatatctg gctcaatttt cccacttatt
    17641 tcactagata atacacgata cgtgtcaggt aaataatcat ttacctccat aaaccgaatg
    17701 gcaatcaatc ggttaaacca cgtataagca acctcttcga ttaactcttg aaaagcttct
    17761 gaataagaag tgacagaagc acgttgtttt agttctgcaa tcaatttgtt tctttgcgct
    17821 atttcttttc cttgaattct tattcctgat ttttcgattt cttgtaaatt gctatcaatt
    17881 ttcttcacat cttcaatttt tgattcatca attcctaaac gttttgcttt agtcattact
    17941 tcatcaatta attttctacg ggcattgacc gcgaaattct ttatcgcttt cttatccatt
    18001 tttctttccc ttctatatgt aacctttgaa aagacaggca cctttttatg cctgtctttt
    18061 cactagaatt gaatcgtgat ttctgtattt tcttgaagtt gttccttcag tttttgatta
    18121 atttcagctg ttaactcatc aatatctgct gctgacttaa tagttttaac atgaacgtta
    18181 agtgttctca ttggtactgt tttctgttga atagtaggtt ttactatcgc tatttcttca
    18241 gcttttgctg cagagtctat ttcatctttt tcagtttcac gaatcaagtt tgctgcttca
    18301 atttgtttac gtgcttgttc ttcttgacgt ctgcggtttt cttcattatc gaaacgttga
    18361 ttaaagatca aatactgttc tgctacgttt aatgataaga aaacaagatc atctatagta
    18421 ttcgcttgtt caatttttgc tttcagttct tgaaattctt ggtcaactat tggtacaaat
    18481 tgatccttaa aatcttttcc ttctaataat ttaaatgttg cttgtttttc ttccgttaat
    18541 ttatttaata tttcctgact ttccaactct aacagttgat tatattgttc aataaattct
    18601 tgaatcaact catttagttt catcatatct gaataaggat cagccttttt caaaatggtt
    18661 ttcatctcat gaatcaattg atcaatttct tggctagaag tttgacgccg acttttttcg
    18721 attaatctaa ttttatcttg tgcattctcc cagatttttt tctgagtact attttgcttg
    18781 tggaaatttt taattttatc atatgtttca gcccagtcga ataattcatc gcgatatttt
    18841 ttgattgtat aaaaaaagtc ttcaacatct gacgtttgaa caattacttc aagatatttt
    18901 tttatttgct catattcata agcactaggg taggtataag tgttttgtaa atcaaaataa
    18961 gagcgtaaat tgtctaattg accttttaaa tatttcttga aattttccat tgtatgttca
    19021 ttgttatttt catcaacaat attacgactt tgaaacagtt ctctaccgat ttcgtcaacc
    19081 aatttttttg cttttggcga aatcaccgcg cttggttcaa tttttatctt ttcactttct
    19141 cgtcgttttt gaataatcat tacaatacta tttggatcat ccatccgtga aatttgtaca
    19201 ttgttataaa gtaaatcaat tttaccagac atgaaaagtt tagcgatgca ccattccaca
    19261 tcaagttctg tgaatccata cggttgtttt gtttcaaaac gatcacgaat tgttttcatc
    19321 gtaatcgtta ggtgatttct cttttgatca ttgataaaat tgaaaatttc ctgaactgct
    19381 tgctcatttg atggatcttc aataatttca tttttgcttt ctttcactaa atataagata
    19441 tcttcttttt ctttaggcgc atcaatttgg tttaatttat ggtatacttc ttcagccatt
    19501 ttctgaagtg ctgattggaa ttttgcttta aagtcggagc ctgtcagttg aatttctgca
    19561 ccgttataga aaaattttgc agatttcaac agttcattta attgatcgtt gatacttgca
    19621 cttagttcct gtaattcacc agatttttcc gaacgaatcg cgtcaaagtt ctttactaca
    19681 ctcgaataag tcgttgttaa ataaagattc attctagcat acattgctat atcatcttta
    19741 aattctttat ctgatggcaa ttctacgaat aataagttac gggaactctt aagcgcaagt
    19801 tctgcctctt gacctgctaa atcagagtat ggtgaaataa aattaactgt catttcagca
    19861 tttccagctt gcttgtatgg acgttgatct aacacttgtg cataagtaaa cgtataccgg
    19921 tttttaaatt tatttccacc tgacatgcct aatttttcat aggcaaaact atttcggtct
    19981 agaacgccgt caaaaattcg tttagatagt tctacgatca aatcactatc tgtaatattt
    20041 tgatttttaa tctcacgagt aatttcctgt tcttcatcag tttggaaaat ataactatct
    20101 aagttcttcc gcacaaagtt ttccttaatt aaaaggtcca atgcttcttc tactcgtttt
    20161 ttagtttcca ggcggtcttc gttgatattt cttatcatca agctcgtaat attttcaaca
    20221 tttggtttga tggcttgatc aacatattta atcatgaata aaattcgtaa gacatttacg
    20281 ttaaagttat ctatatcagt tggtctttga atatgagaat tatttgtagc ttgtgtaatc
    20341 actcggctaa ttgctgtatc aatccaacgt tctaacgagt aataaaactg atctaaagaa
    20401 accaaactgg ttgtatcatt ttccttattt cgttcagcgg cttctttaaa cattgctagt
    20461 aatgaacgtt caccagacga taagtttgat ccttgatagc tgtgattacg aatctcggtt
    20521 agaatttgcc ccaataaatt gaattgatag ggaataaatg gataaacttc tgcgtaattt
    20581 tcagcatctt ggaagagctt taattcagga ctatcaacaa agagtaattt atttttcaat
    20641 tctgtttgaa cagtttcaaa ttgtaaggct aaagaatcat tggcaaactc ttttttcttc
    20701 aaaatacgtt ctttaattac ttcatcggca tttgctgaag ataaactcac agttgtggca
    20761 aaacgatcct tgatcttaga aaaatcatta tcagaattgt tatcaaagtt tgttacgtga
    20821 tcaatcgctt cctgacttgt cactaatacc caagccttac cttcacaaat tcgacccaaa
    20881 tcttccacaa cggtttgtaa ctgcaaagtc aactcacgat taccgccgat atattgtccg
    20941 acctcatcga ctagaaaggc aatatgatgg tctgcttctt tggaatcaag atagctttta
    21001 actagatttg caaagtcatc aatgcttatt aaataatttc ctgatgcttt ttctaaaaaa
    21061 ttatcaatag cggcttcact cataaaatcg attttaagtg ccgcttcttt gatatcatct
    21121 tggatccaat caaactcatg gcgctcttct tgccattttg ccccattaat ctcttcaaaa
    21181 gcagctttga atgcatcaaa cttttcttct ttaattaacc gacgttcaaa atctgctaga
    21241 tgtggaatcg tgcttaatcc ttgcatttcg ttaaatacct ttaaaaaaac attcacgatt
    21301 ccgtttttgt cttttttacc acctgcatca ctttttgaat caatattaaa cagaataaca
    21361 tctgtattta tttgtgagca ccgttccatc gttgccaaag tcagtggatc tttgattttc
    21421 ccatcttctt tgaagtaatc aatggctttc ttgtcattta tttctcgatt tgcgagcaag
    21481 taagaaataa tttttaagaa atgagattta cctgaaccaa agaacccctg aatccaaaca
    21541 cccattttat ctgttggtgt attgatactc cgttggtagg catcaaaaat tgttttaaag
    21601 tgtttttgta gttctttcgt aaccacgtat tcttctaatt cttgttgaac attttcatcc
    21661 tttgcttgac cgaccttaat aactccttga atatttcggt caattggttt tgcaaaaata
    21721 tctttaatta gcatttaatc tcctaccttc catcccaact aaattctaaa tgctcgataa
    21781 taattatcat ctttattggt attaagaatt gtcaaactta attcatcata ttcccctgga
    21841 tacatcatca caactggtac gcgatcaaat acctgatgca ttttatttaa aatagagtgc
    21901 gctctaatta atggatataa ttcgccaatt cctgtcagga acacaactga tttttctggt
    21961 gtatgggcat ccacatattc aaaaaataca ttttcttcct caggtaaatc cattttcaat
    22021 aaacgaccaa tgctttccac taatttttgt aaaccacgtc gttcttccat cttaatcgct
    22081 ttttcagtga aaccgcgttt ttctagatac tctaacataa tcacatagag attataaact
    22141 ttcacttcat aattatcacc aacagatgtt ccagatatat cattaatata atctcttaca
    22201 attaactcgt ctttcggatc gtagtcaaag atataatagc ctacttcatt acctaaacca
    22261 cgattcaata aaaaatcttc tttttgaatt acttcattca acttttccaa atgctcttga
    22321 atcgataatg acattataaa attcctccca gtgctagtac aatatcttta cgattcttgt
    22381 caactaacca ttgaatcaat cgtttatcta ataatatcgg agtcattcgt ccatattctt
    22441 tttcccctct gactaatcct gtttcaatta gattcgtttg ataagcggca ataagccgtt
    22501 taatcgtata atctttccat tttgaaatat cttctgaact tctccgcttc tctgtaaaaa
    22561 aagaacggta atcaactggt gttaattgca aatttccttt acgtagattt tcagcatata
    22621 cctcagtcat aaattcatta aaaagatcat caatcataag gatggaaatc aaattcacta
    22681 acttttgatt atctatattt aactgattga aaagttgcaa tccagcttca tccagttgac
    22741 taactctcct tttcacgttc tgatagattt ctttttgacg gttactgctc actgcaaaaa
    22801 aaatattttc atgttcattt ttctctttaa tttctatatc tgtatatcct tctagtcgta
    22861 attgaatata taacttgaac tctgaaaacc aaaagcgttt agaaactaga ccagcagaat
    22921 attttttatt caatagattg cctcctaatc atctataatt caataagtaa acaagtgttt
    22981 tttgctcatc gttagcattc attttttagt atctactata caatacatat ttattatagt
//
LOCUS       DAYYAO010000046        21520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000046 DAYYAO010000000
VERSION     DAYYAO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.048"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            203..904
                     /locus_tag="ACHARI_001658"
     CDS             203..904
                     /locus_tag="ACHARI_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8442707.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1513..2529)
                     /gene="tsaD"
                     /locus_tag="ACHARI_001659"
     CDS             complement(1513..2529)
                     /gene="tsaD"
                     /locus_tag="ACHARI_001659"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8442708.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2526..2990)
                     /gene="rimI"
                     /locus_tag="ACHARI_001660"
     CDS             complement(2526..2990)
                     /gene="rimI"
                     /locus_tag="ACHARI_001660"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8442709.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2997..3539)
                     /gene="rimI"
                     /locus_tag="ACHARI_001661"
     CDS             complement(2997..3539)
                     /gene="rimI"
                     /locus_tag="ACHARI_001661"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8442710.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3523..4245)
                     /gene="tsaB"
                     /locus_tag="ACHARI_001662"
     CDS             complement(3523..4245)
                     /gene="tsaB"
                     /locus_tag="ACHARI_001662"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8442711.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4436..5416)
                     /locus_tag="ACHARI_001663"
     CDS             complement(4436..5416)
                     /locus_tag="ACHARI_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8442712.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5440..6924)
                     /gene="galT"
                     /locus_tag="ACHARI_001664"
     CDS             complement(5440..6924)
                     /gene="galT"
                     /locus_tag="ACHARI_001664"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8442713.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6936..7925)
                     /gene="galE"
                     /locus_tag="ACHARI_001665"
     CDS             complement(6936..7925)
                     /gene="galE"
                     /locus_tag="ACHARI_001665"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8442714.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8173..8340
                     /locus_tag="ACHARI_001666"
     CDS             8173..8340
                     /locus_tag="ACHARI_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8442715.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8402..10213)
                     /locus_tag="ACHARI_001667"
     CDS             complement(8402..10213)
                     /locus_tag="ACHARI_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8442716.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10210..10575)
                     /locus_tag="ACHARI_001668"
     CDS             complement(10210..10575)
                     /locus_tag="ACHARI_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8442717.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10738..11133)
                     /locus_tag="ACHARI_001669"
     CDS             complement(10738..11133)
                     /locus_tag="ACHARI_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8442718.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11151..12113)
                     /locus_tag="ACHARI_001670"
     CDS             complement(11151..12113)
                     /locus_tag="ACHARI_001670"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8442719.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12113..12325)
                     /locus_tag="ACHARI_001671"
     CDS             complement(12113..12325)
                     /locus_tag="ACHARI_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8442720.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12346..13044)
                     /locus_tag="ACHARI_001672"
     CDS             complement(12346..13044)
                     /locus_tag="ACHARI_001672"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8442721.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13064..13606)
                     /locus_tag="ACHARI_001673"
     CDS             complement(13064..13606)
                     /locus_tag="ACHARI_001673"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8442722.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13738..14742)
                     /locus_tag="ACHARI_001674"
     CDS             complement(13738..14742)
                     /locus_tag="ACHARI_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8442723.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14739..15728)
                     /locus_tag="ACHARI_001675"
     CDS             complement(14739..15728)
                     /locus_tag="ACHARI_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8442724.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15725..16531)
                     /locus_tag="ACHARI_001676"
     CDS             complement(15725..16531)
                     /locus_tag="ACHARI_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442725.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16697..17653
                     /locus_tag="ACHARI_001677"
     CDS             16697..17653
                     /locus_tag="ACHARI_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8442726.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17730..18248)
                     /locus_tag="ACHARI_001678"
     CDS             complement(17730..18248)
                     /locus_tag="ACHARI_001678"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8442727.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18336..18485)
                     /gene="rpmG"
                     /locus_tag="ACHARI_001679"
     CDS             complement(18336..18485)
                     /gene="rpmG"
                     /locus_tag="ACHARI_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8442728.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18713..19159
                     /locus_tag="ACHARI_001680"
     CDS             18713..19159
                     /locus_tag="ACHARI_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8442729.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19354..21249)
                     /gene="asnB"
                     /locus_tag="ACHARI_001681"
     CDS             complement(19354..21249)
                     /gene="asnB"
                     /locus_tag="ACHARI_001681"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8442730.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6479 a         4560 c         3703 g         6778 t
ORIGIN      
        1 tatatgtatt attgttgttt tttatacaca aatcattcgc ttcaaaacaa attattcaat
       61 accattgtat attttttaca taataacaaa ttatatttgt aaagtattaa aacttgtttc
      121 taatgttggt atgttaaaac tttacaaatt ctttaaaaaa agtgacaata aatatagtga
      181 acaataagga gaggactttt taatgcgtgt taataaaatc gattttcact tttcaaaact
      241 cagatatcaa cctgattttg aacatttctc ggatgaagag ttccaacaga tcaaggaaca
      301 tactgttctt cgttcttata aaaaaggaca gatccttttc gacgatggtg atcccagaca
      361 acggttgtat atcttgacca aaggactcgt tcgtttagaa cgttatgatg aaagtgcaac
      421 ttattactac tatgactatg tgaatacaaa taaccttttt cctatgtcag gaatgtttgg
      481 agaagagacc tacacttata cagctcaagc gatgactgat attgaaacat tctaccttcc
      541 tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct atatgataca
      601 aaaaatttct aaagtagttg aaatacatga attgcgtatc caaatcggat tgacctctag
      661 cgcctttgac cgggtaaaac aaaatttatt cattttgaaa gaagaattgg gcgtaccaac
      721 aaaagatggt catatcacca ttccttatcc gatcacctta aaagaattag cagtgaatag
      781 cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt ccgggctgtt
      841 agattatgac aaaaagcgtt ttactttctg taagacagct actactccag ctatgagaga
      901 ataacgcgaa caaaaataag cagtgaccct gataaatatc agggtcactg cttattttcc
      961 tccctaaaac ttctgaaaga tccggcagtt ttagatcact ggacaagact caaagatttt
     1021 ctatgtgtgt ttcagttatg taatattcac tgtttgcaaa aaatactgtt agattaacta
     1081 gatgaagcgt cttgtcctca tccgatctag attattcgat attcctcgac aataaacgat
     1141 tactcagtga gggcaatcag aggaacggat aatgataaat aagctgtaaa aaatagcaaa
     1201 ataacaataa tgattaaaag tatgtaaaaa atagggcttt gacaaaaacg tgtcacagcc
     1261 ctatttcgat taaacggtgt gtacctgtca actcttcggt cacaagtcat aatgttcata
     1321 aacatgagga gctgtttttt gaaattgttc cttgtgaatc agagttaaac gacaggctta
     1381 cgaactcttg attaaacggc gttcaaaagc ttgcatacgc ggtaataaga acagccaggt
     1441 caaaaacatg agaagccgtt tttcctgggc tgttcttatt acttgatgca gggcagcttt
     1501 ttcacaacct ctttattcag ataaaaccaa gcttggatca gcattcaact gatagctagc
     1561 aaaatttctt ttttgcattt ccacatgtgc tgctgctcca atcattgcag cattatctcc
     1621 acaaagtcga agagggggaa tcactagttc tacctcaggg agtttagctg ccaaagcatc
     1681 ctgcaatcct tcacgcaacc cttgattggc cgctacacct ccagctataa tcagttgctt
     1741 gactggataa ttttgacaag ctcgaagtgt tttagtaatc aatacatcga tcacacttgc
     1801 ttgaaagcta gctgctaaat catttttgtc tagatcttct ccacgctgtt gtgcattatg
     1861 caccaaattg ataaatgcac tttttaatcc gctaaaactg aaatcataat tatcttcatg
     1921 gatcatcgct cgcggaaaat ggtaattatc ttttccctga tgagctagct gatcgatttc
     1981 ttttccgctt gggtaagaaa gacctaatac tcttccaact ttgtcataag cttctcctgc
     2041 tgcgtcatct cttgtctccc caatgatttc atatgatcca tcttcttgca tatagactaa
     2101 ttctgtatgt cctccgctaa caagaagtgc catcaatgga aactggaatg gtttaaccag
     2161 acgggctgca tagatatgcc ctgccatatg gtttacagga ataagcggca attggtgtgc
     2221 ccaagcaaaa gccttagctg cagaaatacc aatcagcaac gagccgacaa gtcctggtcc
     2281 ataagtgaca gcgactgccg ataaatcttc tgccgacaca ccagcttcta ccagtgcatc
     2341 ttccaagcat aaggtgattt gttctacgtg atggcggcta gctacttcag gtactacgcc
     2401 accaaaacgt ttatgactgt ttatttgaga agcaacgatg ttagataaga tctctgttcc
     2461 atttctaacg accgctacac tcgtttcatc acaacttgat tcaatcgcca gaatcaattc
     2521 ttcattcatt tcatcgtctc cgtttttaac tttctgctca taatgatcgc atcttccgtc
     2581 ggattgcgat aatatgattt ccgtttacct aatatacgaa acttttctga ttcataaagt
     2641 tttcttgctg cttcattaga tagacgtact tctaaaaaaa catgcagcga atcttttatt
     2701 tgttccgtaa tcagtaattg taacaaatgc cgtgcatgcc cttttccttg ttctgaaact
     2761 gcaacagcaa tatttgtgat ctcgacttca tctattactt tcgagtatcc aataaagcca
     2821 cagatctggt tatcttcagc taaaagcaga taatcagagt gaggctgttg gatgtctgcc
     2881 aaaaactgca ttttcgtcca aggtgaacca tgtggataag cattttcgct aatcatccat
     2941 acgcattctg ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa ctaatctcac
     3001 tcattttcta aaagttttat catatccaac gcatcttctt ttgtttcagt gtaatatctt
     3061 tttttgatcg ttgttgcttg aaaccccaat ttccgataaa gtctttgtgc atcttgatta
     3121 ctcatacgca cttctaatga catcgtctca cagcgattca ttatagcaaa gttctcgatt
     3181 tcgtcaataa gcagcgaacc gattcgttta cgctgatatg ccggtcgaac ggcaatattc
     3241 gtgatatggc aatctaaacc gaacgctctt gaaccgataa aaccaatcat tcttccatta
     3301 tcaaagatac ctagatatag atgaggaagc ggtgacttca attctgatat aaaggcactt
     3361 tttgtccatg gcaattcgcc attatagact tctcgttcga catccagcag ctctttgata
     3421 tcttctgtag tcaattctct tagtacgtaa cgacgatcga tttgttcgac tgttttttct
     3481 gtataaatca ttttatcttg aaaaaagctt ctggcatacg ctctaaattt tttcaacata
     3541 actttcgtct ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta ctcgttttaa
     3601 atagcgagga agaaaagctt gtttctctgc aactggttca actaaagcac ctaattgagc
     3661 cacagttaca ccacttggaa tatcccatgc tggaactgta ttaatcatcg cactaggcaa
     3721 tatttccttt atttcagaga agaatttgtg acaatcactc ccgacgaaat aaatagcttc
     3781 attcagcttt tgtagttgtg tgaacaaatc agaagcagaa atatgtacat cttcaagtac
     3841 agtttctaat ctattttctt tccagcggta tgccccagcg tatacatttt ttcttcgagc
     3901 atcaaacatg ggcacaataa tattatccac accgatgcaa ttagccgcga tggttttcag
     3961 actagatact cctactagct ctttatctaa tgtataagca atcgtttttg ctgtcgttac
     4021 acctatacga agccctgtat aagaaccggg accatcagaa accgcgattc ggtcaatgtc
     4081 ttttggactt atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca gtgttacact
     4141 gtggttgcgt ttgatatttg tttgtatttg tccaagaatt tgttcgtctt ctaccacacc
     4201 gatcgccaaa gtttggtttg cagtatctat tcctaatgta atcatatatt tgatcctttc
     4261 tgctatgaca ttctcgcctt ccattgtagc atactttttt attctgagga tgaaaatacc
     4321 aaaggcaaaa ctagtacaat aatacatgat tatctgctgg cgggcaaatt ttgccccttc
     4381 ctgaaacaca aaaaagctgc ggtatcactc cattttccca cagcttttcc atcttttatt
     4441 tcgttgattc tcgaataatc aactctgttt ccacaataat ctttcttgaa accggtgctt
     4501 tggaattact taattgaaga atcgtctcca ctgctagctc tcccatccat tcggtataaa
     4561 cttttacagt agaaagcgag ggcgttacat atttcgctac actgacatca ttgaagccca
     4621 tcacagaaat atcttctggt accactaaac ctttttcttg aatcgctttc agtgcgccaa
     4681 tcgctaatgc atcattagct gcaaaaagcg catcaggaac atgcggattc gtttttaaat
     4741 attgcttcac tgcttgatat cctgactcga cagaaaaatc tgcttcgata attggacctt
     4801 cgtcgtagcc tttttctgcc aaaacttgtt gaaatgccat aattctggga tcttttagcc
     4861 gctgatggtt tttcttcgta tattctgttc cagcaagcat agcgatatgt tgcgctttat
     4921 gggagacaaa gtgttcaatg actttttgaa cacttgactt aaagtcgaca actagagagt
     4981 cgattccttg agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg gatcctttca
     5041 tttctaataa ctgagcttcg tcgaattttc ctagtgcgat cgttccatct gccggaagat
     5101 cactaatgga atcccacgtt tctttcaata aagtaacgtt tgcttcttct gccttttttt
     5161 cgattcctag gcgaatagat aaataataaa gatcttcaag ctcttccgta tcgtcgtacc
     5221 actggatcag gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt ttcgtataat
     5281 ttaagcgttc tgctgcttca aaaatttttt gctttgtggc taagccaacg gaaagatcag
     5341 gatcatagtt gagaactctt gaaacagtag caggagaaac tcctgccaac tgcgcaatgt
     5401 ctttaattgt tgccatttgt ctctcctcct tttttacgtt tataaaatcg cagtaaatcg
     5461 ttcaaacgct gcctgtcctt tttcatctcg cttgaatact ccagcgtctt ccagtacacg
     5521 tgcaaaaacc tgacctacag atttttgtac gacatcggtg accgtatctt tcgtcaaatc
     5581 aggatgttct ttttttaatt gttctgccca cggacgatga tattcagcca tctggttttc
     5641 ttctcctaat aagaaatgct ctacttctac tagttctggt ttcaaacgag gtggtaaaac
     5701 agcaagcccc attacttcga tcaacccaat gttctccttt ttgatatgtt ggacatcttt
     5761 atgtggatga aaaatcccat caggatgttc ttctgaaaca ttgttgtctc gaaggacaag
     5821 gtccaactca aagtaattgt cccttcttcg tgcaattggc gtaatcgtat ggtgtggtgt
     5881 accatctgct gaaaaagctc tgatagatac tgtttcatcc gaatatgctt tccatttttc
     5941 caatactgag ttagctgctt caataagcaa gtttttctct gttgattgca aacggatcac
     6001 agacatcggc catttcacga ctcctgcgat gacttgcgga taatcttcta gtttgataag
     6061 tttttctatc ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat aatgatcatg
     6121 agaaagaatc gatcctccaa caattggtaa gtcagcattc gaaccaacaa agtaatgagg
     6181 caagacttca gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt tcatcgggcg
     6241 gtgttgttct gataaaatga tcgaatgttc attgtaataa gcataagggg aatattggaa
     6301 tccccagctt tcaccgtcta gattcattcg aataatccgg tgattggtgc gggctggata
     6361 gttcaagcga cctctatatc cttcgttttc catgcaaagc atgcaggtag gatagtctac
     6421 ttttaccgcc tcgcgttctg ctgcgatttg ttttggatct ttttctggct tagataaatt
     6481 gattgtgatt tccagttcac catactccga ctctgccgga aatttgatgt tttttgcgat
     6541 ggctcttgtt ttgatgtagt catttttctt acttaaatta aagaaatact cagttgcttc
     6601 ctctgggctt ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag gtggcggtgt
     6661 gagaaaatcc attaattgcg cttcaaaaat ctcttgttct gctggcaatt ctgaaagaac
     6721 accattttct cttgccttag cgacaagctg atccaataag tctacagatt cttccttaac
     6781 tggtcttact tcaatgcttt ctagagcatc ttctcctacc atccctaaaa gacgattctg
     6841 taaatataag cggtccattt ccatccatcc gccagcttcg atcgcaagtg tggcaaaatc
     6901 acaaatcgtt tgactgattg acatatctca ttcccctatt cttcataacc atttggatgg
     6961 ctgacatgcc aatcccatgc tgttttgata atcgctttga tatctgtgta ttcaggtttc
     7021 cagcccagaa tctctctcgc tttttcactt gaagcgacta atcggctagg atctccagct
     7081 cttcttggtg ctatttctgc tggaatttct tttcctgtca cttctctagc tgcttctagc
     7141 atttccttga ctgagtatcc tttgctgctg cctaaattga agaagttgct ttcatttcct
     7201 tctttcaagt attccaaagc taaaatatgt gcagcaatca aatcttctac ttgaacataa
     7261 tctctgatac atgttccatc tggtgtatca taatcgtctc cataaacagc caaggctttt
     7321 ctttgaccaa gagctacttg taaaatgatt gggacaagat gcgtttctgg tgtatgatct
     7381 tctccgatag atgcatctgc ttttgctccg gctacattga agtagcgcaa agcgacatag
     7441 cgcatgccat atgcttggtc acaccacttc atcatttttt ccatcatcaa tttactttca
     7501 ccatacggat ttttaggatt agctggtgta tcttctgtga ttggagactc tttcggctct
     7561 ccataagttg ctgcagtaga agagaatacg attttattca cgttgaactc gtgcatgact
     7621 tcaagtaaga tttgcatacc gtatacgttg ttgttgaaat acatcaatgg tttttcgaca
     7681 gattctccta ctaaagagct tgcagcaaaa tggattactc cttcaatcgg ttccttttca
     7741 aatacggaac gtagaaattc tttatcccgg atgtctcctt cataaaaatg agcatctgga
     7801 tgtactgctt gtttgtgacc tgtgagcaaa ttatctacca cagctacttg ataccccttt
     7861 tgaactagct ggtctacagc gtgcgaaccg atataaccgg ctccgcctag tactaaaatt
     7921 gtcataaaaa acactccttt gattagtaaa attgtgtttg ttgattctat ataatttaaa
     7981 aaacgaagtt tacttttatg gtgaaaacaa acaaagtaaa atttcgtaaa ttcatatggt
     8041 aaatttataa taaaacattt agtaaacatt ttcaagggtt ttttgcaaag gtttacaaac
     8101 aaacgtaaaa ctagcctttt tttctctttt gttccataat agatataaca aataataaat
     8161 gaggtggatt caatgaaaaa atttgtatct ggaatattag taggaagtct ggcaacagcc
     8221 gctgccgttg ctggtttggt tgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa
     8281 aaagaagcaa tgatcgaaga aaaccgtaaa aaagcaatgc gtaaacgcgt ttctcgttaa
     8341 taattattta aaaatacgca tgaagacttt ctaaaaaaca gataagtcat catgcgtatt
     8401 tttatttgtc agtcaatata agttgtgcta ttttatctgc ctgtctcaga ttccatggat
     8461 ctgtataagg tctagctact gctgcagtta gccaatccac accttcaaca ggtattccat
     8521 agcaataaaa gtcagactgg atttcccctg aagcatcaat cacgcgattc gtacctcgct
     8581 caacatctat agcttctgaa tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa
     8641 caagtttgtt ttccttcaaa ttttttatga caggattact tgaacgagaa aagcttgttt
     8701 gtggcaatcg agcttcaatc aaatagcgac tgctgatttc tttatcttgt agagaaataa
     8761 catagcggtt gtgcttttgc tgaatatcaa atggcggttc gatcaaatga aaaatacccg
     8821 catctatcag tgctgctaat tctttcgtcc gaatgattgg cggaccgatc gttaaaaagc
     8881 tatttagatg agtaaaccat ccccatagta tttctttgta ttccttagca gaaaaaacgt
     8941 gccattcaat agcctgccgg attggatcac gccagtcttt taatgcatca aatgttgaag
     9001 caaatgctcc tgtgcaattg cctttttccg cttcttttat ctgcccttca ataaatattc
     9061 ggctagcttc tgaaaagggc tcattctgat aggcaagagg agtttctaaa tacgaccaat
     9121 cccatctata agggattagt gctggatgct ttccgagaca ctcctctgaa gagagggaga
     9181 gaaaatcatg ctcaaaattt ttctttggaa tcggcaagtg atattcttcg atgattttct
     9241 tgtaataaaa caactcaacg tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa
     9301 atcccgactg atgccattgc tccaggtttt cttttgttag taaccgtggc catgccattg
     9361 ctccgccttg cttttgattt cttcctcgag ggtaataagg gagccccctt cttgatccac
     9421 tgtatacaat cggttccttg cctgatggat gatagaccag ttttcccttt tcgttctcga
     9481 agcgccctcc tcttgatact gtaaacaatc cgacataatc aaaaaaactt aagcctagac
     9541 ctcttaaaaa gacattttct ttaggcggta tcttcgaaag atctacatct gcaggattag
     9601 aaggaagctg atacagtaaa acatttttct ttgcgtagtc tacccacact ctttcttctt
     9661 ctgtctcatc attttcccag tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt
     9721 gctgatctgt ctgtaaaaga taaccatctt ttcttttgct tacttttttt actaaagaat
     9781 aaacaaactg aatagaagct ggaaattctt cttgcaactt tgagaaaaac cagcgttgat
     9841 agagtccgta taaacatcga gtagattgct gatctttttc cagacgattg atttcttcta
     9901 taattagttg cttatttttg gttaccgtct gttcgatata atctgtagca ggtcctttac
     9961 tccattgaaa caagttgggt ccctgtgtta ccaatcctcc actggacaat gtttcatctg
    10021 taaaaagcgt cacttgttga gaaacagaat tcattaataa ctccggtggc tgattcaacc
    10081 gccaaactcg acctccagga ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt
    10141 cctgattatt atggagaatc ctttctgcta cacttaatcc tctaggtccg gcaccaatga
    10201 tccctatttt catttgcatg cctccatttc gataaatttt ttcaataaaa cagcatggtt
    10261 ctgatgttca ttttttgcac cataaacaaa agtaatgact ggatgttctt tcataagttg
    10321 actgagttta aaaatagctt gcttcttttc ttcttcgaaa atcaattctt gctggtattt
    10381 ctttgcaaat gaagcaaatc gttctggtac atggtcaaaa gttttgcgtg cttcattact
    10441 aggtgcaatt tctttcaacc acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg
    10501 ccagaatcta tccacgagta ttctatagcc atcctctttt tcagcatcaa ggtaaacacg
    10561 ttttaatttg atcatagttg tttccttctt tctacctttt cgcttagctt atatatgtag
    10621 ataatagcac aaaataaaaa aaatgaacgg cacaactatc cattgcgccg ttcatgtgat
    10681 cttggtcaaa atctaatgat tgattcggtt tttaaagctg tcatacaatc atctgtttta
    10741 tactttttta cttttgattt ttccagacca tccgtcatat ccacccttaa gtaaataaat
    10801 ctcttgataa cctgctttac gtaatttatt tgctgtacgg atgctcaaac ttttcttttg
    10861 atcgtaaata tagacaggct ggtcttttcg aatagatcct aatgaatctt tcaaaacagt
    10921 gtaagggatg tttcttgcac ctaaaatatg cccagcatca aacacatctt tttcgcgaac
    10981 atcgattact tgtgcttttc tcattccttc tcgaaattct tcttctgtca aagtcgttgc
    11041 tgaacgtttg gccattactc gtagatagac ttcccaaagg atgattgcaa gcacaatcaa
    11101 tagcaaaatg atattaatta cccataaaac catgtaatgc ctccgttttt ttaagcaaat
    11161 ttcaaggcta atgaggcagc accaatgaca ccagcttcat taccaagctc tgctaatttg
    11221 atttttgttg attctcttac ttgcgggaaa gtaaattctt taaaatattt ctctacacgg
    11281 ctacgtaaaa attctcctgc tgccgatact cctccaccta acacgatact tgacggattt
    11341 aatgtattcc ccaagtttcc gcaagctaaa cctaagtaga agcatacgcg atcaacaacc
    11401 atcaatgcaa acggatcgtc ttcttgagcc atttcaaaga tatctttact tgttacttct
    11461 tctccgttgt ctagcattgc tttcagtttt gaatcccctg cgtattcttc tgccaagaaa
    11521 cgacctaggc gtaccacacc tgtagcgcta gatacagttt ctaaacatcc gcgtttaccg
    11581 caagtacatt cgaacccttc aggatccaca gtgatatgtc cgatttctcc tccagctccg
    11641 ctcacgccgt gaatcaaatt tccgccagca acgatacctc cgccaacacc tgttcctaga
    11701 gtaacgaata caacgtctgg atcgttttct ccagcacctt tccatcgttc acctaaagca
    11761 gcaacattcg cgtcattatc gatagcgaat ttgattcctg ttcctgcctc gatcagtttt
    11821 ttcacaaatt gaacttctgt ccagtttaag ttatacgcac caataaccgt tccagcttcg
    11881 ctgtccacgc ttcctggggt acccatgccg attccgataa agtcttcggc tttcatatta
    11941 tataaattca aacgatgatt gattgattca atgatttcag gaacgatatg cttaccatca
    12001 tccaaaatat tggtatcgat gctccatttt tgctgaatct ctccttctgg tgttaaaatt
    12061 gcaaattttg ctgttgttcc acctaaatca atcccaataa tcttcttgtc cattacaaat
    12121 ttcctttctt tcggctttct tcaattcggt gctctctttt taatatgcgc cacgctgtca
    12181 tgtatgtatc atgatcaatc aaacggctat catacagatt acgcaattcg atagtcatta
    12241 attcaatgtc atagatgcgt gcacccacat agataaagat gccaaatttt ttgaacagct
    12301 gctgtacatc atataagctc tccatcttta agcacttcct tcatattata caagtaaacc
    12361 atattttttt aaacccagta aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga
    12421 aagaatcctt tggtgtatcg aatagcttgt ctgttgtttc ggtagaccca aaacattccc
    12481 taacactaat ccgccaatta gcccaccgac atgtccccaa acatctacag aggtatccag
    12541 aagaccaaaa acaaatgtca acacaataaa taaaagaaaa tgtctgacta gctgctggat
    12601 cgctgggtta tgtttaaaat gaaaccctaa gataaacaac gcaccgaaca aaccgaatat
    12661 cgaagtactt gcaccaccag acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt
    12721 tcccattatc ccgctgaaca tataaatcaa gaaaaaacgc caatggccat agattgcctc
    12781 gatttgctgc cccataaaat acagtatcag tgagttcatt acgaaatgca tcagtccaaa
    12841 atggatgaag attggtgtca ctaaacgcca ccattcattg aagtgaacaa gataaggacc
    12901 atacattccg gcataataag ggatctcaag tcgcggaaat aattccatca tgaggaagac
    12961 cgctgtctgt atccctaaaa aagcataggt ccataacggt cgtcttaacc atgcacgcaa
    13021 ttttatttgc tgttgataat tcatgaaaac ctcccatttc attttagtca ataaacaatt
    13081 gttggatctt ctgatcaaag ctctccggtt gccattgttc attcaattgt tcacgaaaaa
    13141 caaggctaca tgttttttta tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa
    13201 aaccgattcg ataaccggct ctattgtaga taatacctgg tacaatgagg agatcgatca
    13261 tctccggcaa atagagcttc tctgattgtg gctcaagaac accaaaactc gaacgtacaa
    13321 gaacggactg ttcatccatt tcataaaacg ccatctgacg tttcggcaaa gttttaggta
    13381 caactatttt cttttgttct ttttctgctc gttggataac cggctgtgta tccagttcaa
    13441 attcattcga taaagtcgtc cctatcactt ttgctgactt ccagtactcg ctggcgaata
    13501 gctgtttata gattcgctgt tctttttcct ttttcttgcc acttttggcc agatttttca
    13561 gcttttcgat tcctttccgg cgtaatctta ctttcaattg gtccaaaaac tcacccttcc
    13621 ttctctcata aaatggtata ggaaaaaaag aaaaatagca atcgaacttt caaataaatg
    13681 cataaataga ataaaaagaa gacctagaaa tgattggcct tcctttttta atgaatttca
    13741 actgttcagc gcgtgttttt gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa
    13801 aagggcaatc aaaattcctg taggtacttc cccacttggt agaatcactc tagcaaccat
    13861 atctgcacta agcagaaaaa tggaaccgaa caaaccgctc agaacaaagg tccagttatt
    13921 ttctttacgt acgactagct tcgcagcatg aggagctagt agtcctacaa aagataaact
    13981 acctgtcacc gctacactac cgcttgccaa agcaacagct aacaacaaaa ggattttttg
    14041 acttttagcc acgttaaggc ctaatccttg cgcgcgttct tgaccaaatg atagcaatac
    14101 aatcctctgt gcataaagtc ctcctagcgg aattaagata accgcccatg gaagcaatgc
    14161 ccaaacataa ggccagctcg caccccagat tgatcccgct aaccatgcat tcacaaatcc
    14221 ataattttcc ttagataatt ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt
    14281 aacggcaata ccggacaata agattcgatt catacccaaa aagccatttc gatcataggc
    14341 cgcaagatag acacagcaag cagctaaaac accacctaga aaggctacaa ctagcaatag
    14401 aaacggcagc gaatagtgcg aaaagaaacc taaataaagc atcactaaaa atcctgctcc
    14461 ggcattaatg cccaatatac tagaatctgc caattcattt cttgttactc cttgcaagat
    14521 ataaccagaa acgccaagac aagctcctgc taataaactg atcagtaatc gaggaactcg
    14581 aaattctcta acaatcagcg ccaaagaaga tgattctttc cctatgaacg tggcaaaaag
    14641 ctgatccagg taaataaaag gcgtaccaat cattaaacta cctatagaca ggatgacaag
    14701 tacaaaaagc aagaaataga acgtccaata tatttttttc accgttgttc cctccttact
    14761 tgtatcagta aaaacggaac gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa
    14821 gggggattga tcatcctcgc tgtaaaatcc gccaacataa ccaatccacc gccaactaaa
    14881 aaagagacag gtagaacata acgatagtcc gttccaacca ttccgcgaac aactggcgga
    14941 accaacagcc caataaatga aacaggtccg actaacgaaa cggcaatggc tgctaacact
    15001 gcaactagac agaaaacaat caaccgcgtt cgtttaggat gacttccaag agctaatgca
    15061 tgtgtttcac tcaaatgaat caaagatatt gcttttccta atgaaaaaga aaccaaaaca
    15121 gcaagtatat ataaaggaaa caatactgct aattgtttcc atgtgatatt ggcagctccg
    15181 cctacaaacc aaaaagcaag atcctgctgc agattaaaga attgggtaag tgcttggctg
    15241 atagcagtaa aaaaagaact aatagctacg ccagctaaaa tcagctgtaa aggattcatg
    15301 cccattttcg aaaactgcaa aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc
    15361 aatgacatta gaaacacaga aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc
    15421 attgccaacg aagcacccgc gttgattcct agtaagcctg aatctgctaa aggatttctt
    15481 gtcactcctt gcatcaatgc accgctcaat gcaaaacaac tccctacaag aaatgctccc
    15541 gccacacgag gcaaacgaat atttcgtatg atttggtgac tttgttttga tctatcaaaa
    15601 tcaaacaacg cctgccaaaa atctgctttt tccacagtcg ctgcgccatt tgaaagagca
    15661 acaaaaaaaa taatcaaaag aacaaaacag accaaaagca ggagtctatt tttcttcata
    15721 gtcctcataa ttgatcctcc tgctcttttg gcaacaaatc ataggttaga aaaactggtt
    15781 tatttgttct tggaagcgtg acaatcgttg catcaatctc aaatattttt ttcatacact
    15841 cttttgtgat cacttcttca ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta
    15901 ataaataatc tgaaaaacgt gaagcatgat taatgtcatg aatcgacatc aagatcgttg
    15961 tttgtttcat ttgattgatt tcagtcaata actgtaggat ctctaattgg tgtgccggat
    16021 ctaaaaatgt tgtcggttca tccaagatca agatatctgt ttcctgagca agcgccatag
    16081 cgatccagac acgctgggtt tgtccaccag acagcgttgc gatatcccgc atacgaaatc
    16141 cagctagacc tgtagcatcc aacgcccact caattgcttc atgatcttgc gtgtccatgc
    16201 cactgaacag tttttgataa gggtatcgtc cgtaagatac tacttcttct actgtcatac
    16261 ctgtagggtg ttcactagtt tgtgctaata atgcaatttt ttttgcaagt tcttttgaac
    16321 tgtatgtaga gatatcagag ttattgacaa tgatctttcc tgatttcaca ggtagtatac
    16381 gggtaaaact tttcaataaa gtagattttc cacttccatt tggcccaatc aaactagtga
    16441 ttttcccttc aggaatcgta acagacatgt tttcaatgat ttttttgttt tcgtagccgg
    16501 ctgcaatgtc agctgctctt aatctcgtca tttcacccat cctttcgctg aaaatcattc
    16561 tcaattatga cgctgtttca acatttcgtc aagcgctact ttcatatcta tagccaaatg
    16621 aagcggttga cagcgttcga aatatcagga ataataacca atgagaaaga ttctcaatta
    16681 tgttaggagg aaagaaatga aattaaaaac aaaagtattc acattatcta gcatattgct
    16741 gctggctgga gtactgagcg cttgctcccc ctcttcagat aaagaaacag ctacaactgc
    16801 caattcgacg acttcatcca atagtactca tacagtcaca gatacacttg gacataaagt
    16861 agagattcca aacgcgccca aacggatcat cggcagttac ttagaagact atctgattgc
    16921 actaggggaa aaacctatcg cccagtggac tgttggcagt ggttccattc aagattatct
    16981 gcaagatgat ttaaaggatg tcccaacgat ttcttatgac cttccttatg agaaagtgtt
    17041 aagctttgag cctgatttgc tgttgatttc ttcttccgct actgtcgaag gcgggaaata
    17101 cgaacaatat agcaaaatcg cgccaactta tgttgtaaaa aacggaacag atgtcacatg
    17161 ggaagaacaa ctaaaagatg tcggcaaagc tttggcaaaa gaaaaagagg cagaaaaaat
    17221 catttccgat tatcaaaaat ccatcaagaa aactcgagaa gaactagcag ataaaatcga
    17281 gaacaagaca gctgcagtgt tgtgggtgac caacaactca gcctttatgg tcagcaaaaa
    17341 tcggtcaagt ggaagaatcg tatacgacga tctggaattt ggtgttcctg atctagtgga
    17401 agaggtctcc aaaaaagcca cttctgactg gtctgctgtt tcctctgaaa agctagctga
    17461 attagatgct gactacatca tccttgtgaa cagtgacaaa gatgctgcca tgttcaatga
    17521 accaagctgg aagaatctaa aagcagtgac agacaatcat gtactgcaat tcggacctga
    17581 atctagttgg ctatataacg gacctattgc cagtcaggaa atggtcaatg atatcaaaga
    17641 aaaactaaat tgattttaac taaaaaaagc atcttcacga ttagtcgtga gatgcttttt
    17701 ttcttattaa caaactagac gcatagattt tattcttctt ttgaagcaac cctatttttt
    17761 actctttccc atttttgaaa acgatgggca tatagatttt cttgatccgt tatcccctca
    17821 tactcctcta taagggcgaa gtctgaaaaa tcaatattcc caatatacgt atcgccttca
    17881 aacgtgctat cgatcaatgt tcgccagatc cgaccagtgt aaggaagaag ttcttgaaaa
    17941 atccgactac cacccgaaac atagatatct tctgagatcg tttgactata cgacaaaagt
    18001 tcatcgatcg aatgcattac ttccgcattt tctttcgtgc gaaaatttgg ttgcgttgtc
    18061 aacacgatga tatggcgata aggaagtgaa agatccccca ttccttcata ggtctttctt
    18121 cccatcacta aaattcgatc cattgtatgt tcgcgaaaga aacgcatatc atttggcaat
    18181 cgccatggta acaaaccatt tttgccgatc agaccatttt tatcttgtgc ccacatggaa
    18241 ataaacatat tatccgctcc ttatgatttt agatgttttg agataaaaaa agtctcaatt
    18301 ctttgaccag caaggaattg agaactttaa aaacattatt ttgtttcacg gtgcaaagta
    18361 actttgcgtt cgcgtgggca atatttttgt ttttccaaac gatcaggatt gttacgtttg
    18421 tttttgcttg ttagatagtt acgttcttta caagaagtac actctaaagt gatgtttacg
    18481 cgcatgtttt tccctcccat ttttacaaga atcctctaag atgactctca gacttgatta
    18541 tcttaccatg attttttcta gattgctagt gtttttcacg attttgttaa gtaaaactac
    18601 ttgacgataa atacaacggt tttacttctc aaacaaaatc tttttctttt tgtataaata
    18661 cccttacact tgtttatata gattatgttt tttaataaag ggggatacta atatgccatg
    18721 gaacatgaag gactttcctg catcaatgaa aaacttagat aaattgacaa gaaaaaaagc
    18781 aatcgacatc gcaaatgcat tattagatga agggtaccct gataaccgtg cgatcccaat
    18841 tgccattgat caagcaaaaa aatggtatga agatgctagt gaatcagaaa aacgaacttt
    18901 cgaaaaagaa aaaaatccaa gcaagacgga tgaacacgat acgaatcctc gtgctggaaa
    18961 actgcttgat tcagatgtga tcgtagaata tgaggaagaa caatggatcg tgaaatctaa
    19021 aggggcaaaa aaggcaagca accattttga tacaaaaaaa gaagcaatcg aaaaaggaaa
    19081 gcaagtagca caaaacaaag aatcaacttt gttcatttat aaaaaagatg ggacaaaaga
    19141 aaaagaaatc gattattgaa catatgtaaa ggggttgtga cgaaaatcgt gtcacaaccc
    19201 ccttatgttc gaaacaatgg tgttcctctg ccaactccga accataagtc ataatatcta
    19261 taaaacggga agagctgttt tttgaaattg tttcttatca ctcagtgtta aacggtagga
    19321 gaaaacctct tttttattga tatttgattt tcatcaaaca gtcacatagt cgattgcttc
    19381 tgcttgcgga atcgtttcat ctacaaagta gactttatac catgtcaaga aactgaagat
    19441 tgtccagatt tttctttgct cttctttacg tccttcgtga tggtcatcta gcaacttcat
    19501 gattttttct tgatcaaaaa attcttttgc aaaatctgct tcgaataatg ctttgacttg
    19561 ttcgtagccg tgttcttctt tcaaccaagc tttgattggc acagggaatc ctaatttcac
    19621 acgattcgac cattcttccg gaagatgttt gttcgcagct tgtctgaaaa tatcttttgt
    19681 gttgttttga ttcaataaaa acttacttgg tatttgttga gccaatttca tgacttcgat
    19741 atccaataaa ggtactcgga cttccaaaga acttgccata gaaagtttat ctgcctttaa
    19801 caagatatct tttggcatcc attgatgaag atcgacatat tgcatcttat tgacttcatc
    19861 tttgattcct tctgttttct cgtaatgaac agtcatgatt tcatccacag atggtgctgt
    19921 ttgatatttt ggtgttaaca cttctgatgc ttctgattct tcgaacactc gagcatgacc
    19981 gataaaatat tcttttgcag gagcaagtga ttcatataaa tggatccgtc catggaagtt
    20041 tttcattttt ccgattttac gtcctaaatt atagcgtgtt ccttttggta attttttcaa
    20101 tccttgagca atcacacgga tgaattttga attcgtatgg aatccatacg tttggtatcc
    20161 cgcaaacagt tcatccgccc cttctccaga ttggactacc cgcacacttt gtgaagcaag
    20221 atttgctaaa aagtacaaag gtacgcaaga aggatttgaa tcaggttcat ctaagtgata
    20281 ctggatcaaa gggaatgctt tgaacgacat gtctccgtcg atcactgctg ctgtattatc
    20341 taagtccaat agttcggtta gttttctggc ttcaatcgct tcattatagg ttttatcatc
    20401 aaacccgatt gagaaagaat gatctggttt caatacagag gttacataac tggaatcaac
    20461 cccgctggat aagaaagaac caacttctac atcactgacc gtatgtgctt caatagaagc
    20521 ttgaacggtt tcgtcgattt tttcgatcca ttcttgtcgg gaatgcgttt cttttgtttc
    20581 aaaatctgca tcccaatatt gctgaatgtc tatttttcca tctttatacg tataataatg
    20641 gccttcaggc aaacgataga catctttgaa gaatgtttcg ccgtttaatg gagaatattg
    20701 gaaagtcata taaggtttca acgcttcttt gttcaattct ttgttaaaat caggatgagg
    20761 cagaaaactt ttgatttctg aaccaaacat aaatgttccg ttcatttctg cataataata
    20821 tggtttgatc ccaaagtggt ctcttgcacc gaataactca ttcttttcat tatcccaaat
    20881 agcaaaagca aacattccgc ggatcttttg caataattct gttccccatt cttcgtaacc
    20941 atgcaaaagg acttctgtat ctgcatgtgt ttgaaaaacg tggccagctg caatcaattc
    21001 ttctctcaaa ggctggtaat tatagatttc gccattaaaa ataatgatct ttgtccgatc
    21061 ttcattgtaa ataggctgag taccgccctc taagtcaata atacttaatc ttctgaatcc
    21121 taaagcaaca tgtttatcga tatattctcc tgaactatta ggtccacggt gaacgattcg
    21181 atccatcatt gtattaataa ttgttttttt gttgtccttg tcgttaacaa aacctacaat
    21241 accgcacatt taactttcac atcctaatat aaaattactg tttatttcat tcatatgcat
    21301 cttatcatag aataaatagc ggacgaatat tttctctctt acaatttcgt cacgtgaaaa
    21361 caaaagatga aagtttctgt ttttacagaa aataaagtcc caatatctaa tttaccatgt
    21421 tttctttttt gaggctacct tattctcttc gtgttaaaaa aatattagtg gatgctattt
    21481 ccaaaaaaac cagcaaaaaa agacacgacc taaagatcgt
//
LOCUS       DAYYAO010000047        21501 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000047 DAYYAO010000000
VERSION     DAYYAO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.049"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..1532
                     /locus_tag="ACHARI_001682"
     CDS             60..1532
                     /locus_tag="ACHARI_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8442731.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            1615..2403
                     /locus_tag="ACHARI_001683"
     CDS             1615..2403
                     /locus_tag="ACHARI_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8442732.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(2470..3138)
                     /locus_tag="ACHARI_001684"
     CDS             complement(2470..3138)
                     /locus_tag="ACHARI_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8442733.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            3253..4965
                     /locus_tag="ACHARI_001685"
     CDS             3253..4965
                     /locus_tag="ACHARI_001685"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442734.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4968..6737
                     /locus_tag="ACHARI_001686"
     CDS             4968..6737
                     /locus_tag="ACHARI_001686"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442735.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(6942..7508)
                     /locus_tag="ACHARI_001687"
     CDS             complement(6942..7508)
                     /locus_tag="ACHARI_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8442736.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(7508..8464)
                     /locus_tag="ACHARI_001688"
     CDS             complement(7508..8464)
                     /locus_tag="ACHARI_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8442737.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(8464..9120)
                     /locus_tag="ACHARI_001689"
     CDS             complement(8464..9120)
                     /locus_tag="ACHARI_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8442738.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            9490..11508
                     /locus_tag="ACHARI_001690"
     CDS             9490..11508
                     /locus_tag="ACHARI_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8442739.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    11578..11784
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11935..14349
                     /gene="leuS"
                     /locus_tag="ACHARI_001691"
     CDS             11935..14349
                     /gene="leuS"
                     /locus_tag="ACHARI_001691"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8442740.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            14697..16250
                     /locus_tag="ACHARI_001692"
     CDS             14697..16250
                     /locus_tag="ACHARI_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8442741.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(16631..18415)
                     /locus_tag="ACHARI_001693"
     CDS             complement(16631..18415)
                     /locus_tag="ACHARI_001693"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442742.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(18430..20151)
                     /locus_tag="ACHARI_001694"
     CDS             complement(18430..20151)
                     /locus_tag="ACHARI_001694"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442743.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            20251..21012
                     /locus_tag="ACHARI_001695"
     CDS             20251..21012
                     /locus_tag="ACHARI_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8442744.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         6851 a         3862 c         4401 g         6387 t
ORIGIN      
        1 aaaagatagt aattatttat ctatttttag atcctgtcat aatgaaaggg aggaataaga
       61 tgaaaaaaga aacaaatgtt actttggtca cagtgagtat tttcattgca acgttcatga
      121 cggctattga aggtacaatc gtaacaaccg ctatgccaac gattgtcggt tcattacatg
      181 gaatggaaat catgaactgg gtattttcga tttatttgct gacaaacgct atgcttactc
      241 cgatttacgg aaaacttgcg gataaagtgg gacggaaacc aatctttatt tttgggacat
      301 tattgtttat tatcggttcg ttattttgcg gtttatctca aagcatgatt gggttgattg
      361 ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat gccagtagct ttgacgatca
      421 tcggcgatct ttattctatt gaaaagcgag caaaagtttt aggtttgaat agttcagcct
      481 gggggattgc cagtgttttt ggaccgcttg cgggaggatt catcgttgaa acgatcagct
      541 ggcactggat cttttttatc aatgtgccga tcggaattct attaattatt ttgatttggc
      601 tatatttaaa tgaaccaaaa atcatacatg agtctaaacc aatggatatt tttggtagtt
      661 ttagtttgat ggccatgctt gtctcattgc tgttagggtt ccaattatta ggagacgaag
      721 gaatgagtcc gcttgtttct gctttgctgc taggtagtat cgtaatgtta ggtatttttg
      781 tttttgccga aaaaagagct aaagatcctg tcatcatgat gcatttattc aaaaatccac
      841 tatttgttgt ggtcaacttg gctgcagcat tggctagtgg ttttttgatg ggaatcgatg
      901 tttatatccc tatgtggatg caaggtgtat tagggctgaa tgcagcaatt ggtgggctgg
      961 tattagcacc attatcacta gtctggatgg tcggttcttt ccttgcaagc aagtggatga
     1021 ctcaaatgaa aactcattgg gtcttgagaa ttggactact aatcgcagct attgggggcg
     1081 cttggttgta tcttatgcct ttcagaagta actatttatg gttttttgct atatcaacgg
     1141 tcatcggtat aggcttaggt gtgaccatga ctacctcgac tgtcagtgca caaaatagtg
     1201 tatctcgtga ccagcttggt gtagcaactt cttttaatac gttagttcga acaattggtc
     1261 aaacagtcat ggtttctatt tttggtttat tgataaatgg ggtaactaaa cgagaacttg
     1321 cagcagcaaa tttaacaaat gatgcagata tcatgaataa actggtgaat ccacaaacgg
     1381 ctaaatatat tgatgcggat attttgttac cattaagaaa aattctttat gatgggttac
     1441 attccgttta tctagttggc ttgttactcg tgatcattgc tttggtctta acgcttttcg
     1501 ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt aagatatgta cgtagccgaa
     1561 tcattcaaat ttaaagttat tcaaaaataa cttcttacta aggaaggcga aaatatggca
     1621 tttattcaag caaatattta ttcgaatgtg ttagaaatgg aagtcaatct aaatgtgata
     1681 ctaccgcaaa caacaaaaaa ggtgattggc accagttcag agcaagagac aacggatata
     1741 cctgttttat atttacttca tggaatgggt ggcaatcata gtgtatggga aagacgtacg
     1801 tcgattgaac gttatgtagc aaactacgga ttagctgtca tcatgccatc tactgatctt
     1861 ggctggtaca cagatacagt ttatgacatg aagtattgga cattcattgc agaagagcta
     1921 cctgagattt gtcatgaatt attcccgcaa ttaacaagaa aaagagaaaa aacatatgcc
     1981 gctggattat cgatgggcgg atatggcgcg ttaaaactag gccttgcaaa atcagaaaat
     2041 ttcgcagcag taatctctct ttcaggcgct gtttctgtag gaaatcgaat ggatgatctt
     2101 ttgatggttc gtaaagcatc tttttgggaa gggatctttg gaccattgga tcaaatcgaa
     2161 ggttcaaaaa atgatccgca atacttattg aaagaactag taaaaagcgg aaaaccagtt
     2221 ccaagatttt atatggcttg tggggaatct gattttcttt atcaggcaaa tcaggaaatg
     2281 gtatctgcta tgcaggaaca aggcttggcg gtcacctttg aacaaggacc tggcgatcat
     2341 aactggatct tctgggacca atggattcaa cgtgcattgg cgtggctaat ggaagaaaaa
     2401 taaaaaaaga gtcttaaaaa gatagctcct atagagatca caaacatagt gatctctata
     2461 ggagcttttt tactgctcgt tttctcttct tttattttgt tggcataagc cttcaatata
     2521 ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat tctgcatggg actgcatact
     2581 ttctttaaat ccctctaatt gattttcaac tacataatta cgcataaata gaactgtttc
     2641 tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc tgctgttcat gctgctgatc
     2701 cattaactgt ccaatcaata aattgtggag agaggcaatg gcctgatgac tttgggtgac
     2761 tgaaaatcct tcacgaacaa gtaattggat catttgctcc aaatgttcta atcgactcgg
     2821 aaaagcgggc acagtattga tttctaattc tgccccacaa gggaaacgtg tataaaggtc
     2881 atagtaattt tccatatact gcagcaactg ttcattccaa ggcaaagttg gatctggaag
     2941 cgctaattct tcttccattg tttcagccat tttttgcaag agctgttgtt tgtttttgaa
     3001 ataccaatat aaagcaggtg cttgaacatg aattctgttt gcgatgtttc gcatggaaag
     3061 ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg gcctgaatga tgattgcttg
     3121 agaaagcttt atttccattt tatcaacctc gtgtattgca ttatcttttt aaacatggta
     3181 aattgttagt cggtctaatt ttacagtgtt aaattagtcc tgtcaattta aacaaagggg
     3241 tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa aatataaagt atctgttttt
     3301 atttccttgc tgtccgtagc agtcatggtt gcagcagcat tatggcaacc taagttacta
     3361 cagcaagtat tggaagctat tattacagaa gataatgatg aaatgaaaac aattggtatt
     3421 tacctgatca gtctggcttt gctaggatta gccgcaggtg taacgaacac gattttctct
     3481 gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag aagcatttcg aaagatccag
     3541 acattttcat ttggaaatat cgaacagttc tctgctggaa atcttgttgt tcgtctaaca
     3601 aatgatatca cgcaaatcca aaatttagtg atgatttctt tgcaatcact ttttcgaatt
     3661 ccattcttat ttattggtgc cttcatttta gctatgatca cgatgccgca attatggtgg
     3721 atcatcgtag cgcttatcgt tacggtattt ctgattacgg cactttcatt tacaaggatg
     3781 ggcaagcact tcatgatcat tcaaaaactg attgatcgtg tgaatagcat tgcaaaagaa
     3841 aacttactag ggattcgtgt agtgaaatct tttgtacaag aagaaaacca attgaagtca
     3901 ttcagcaaag taagtgagga cttaacaaaa cacaacatca tagtcggtac attattttct
     3961 gtgatgatcc cgtccttcat gctggctgcc aatttggctg tggttggggc gatctttttt
     4021 gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg gaattgcttc attcatgaac
     4081 tacttgatgc agattatgat ggcaatcatt attggcggaa tgatgatgat gatgacttca
     4141 agagcagccg tatcgctgaa gcggattgcg gaaattttag atgctgaacc agatcttact
     4201 tatttagacg ttccagaaca agagttgacg ggttctgtca cttttgacca tgtttctttt
     4261 cgttatcctg gtgaggatac agatacgctt aaagatatca gcttttctat caaacctgga
     4321 gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt caacactagc acaattgatc
     4381 cctcgtctat ttgatccgac agaaggcaaa gttgaagtag gtggtgtaga tcttcgtcaa
     4441 gtaaatgaaa agagtttaag acaaacggtt tcctttgttt tgcaaaaagc aattttattc
     4501 tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga atgcaacaga aaaagagatg
     4561 gaacatgctt cttctattgc tcaagcaaaa gaattcatcg aaaagttatc tgatcgttac
     4621 gaagcaccaa tagaagaacg tagcagtaac ttttctggtg gtcaaaagca acgtttatcc
     4681 attactcgtg gcgttatcgg taatccaaag atcttgatcc tagatgacag tacgagtgca
     4741 ctggatgctc gctctgaaaa gctggtccga gaagcactcg atcgagattt gaagggaaca
     4801 acgacgattg tcatcgcaca aaaaattgct tctgttgtca aagcagaccg catcttagta
     4861 ttggatgaag gcagattagt cggcgaagga acccatgagg aattggtagc tggaaaccgt
     4921 gtctatcagg aaatctttga aactcaaaaa ggaacggagg agtaagtatg actgatctaa
     4981 taaaagcaag taaatttttc tatcactatt tgaagcggta caaactctcc tttttcttta
     5041 tctttatcac agtgatcatt gctacctatc tacaagtaaa ggcacctcaa tacatcgggg
     5101 aagcttttca agaattagct aattacgtag gaggcttgat gcaaggcgta gacgataaaa
     5161 gcaaatttgt ttctgtaata tggaaactct tgctgtttta tgtactagct agtgcagcca
     5221 actttatcta tagtattttg ttcacacaag tagtagggaa gtcaacgaac cgaatgcgta
     5281 tcggtctatt caacaaattg gaaaaattga cgatccgttt ctttgattct catcaagacg
     5341 gtgaaatttt aagtcgtttt accagtgact tagataatat tcaaaatagt ttgaaccaag
     5401 ctttgcttca agtcatcacg aatgctgttt tacttgtcgg gatcttgatc atgatgttcc
     5461 gccaaaatgt agagttagct tgggctacaa ttgcttccac tccagtagcc gttttgatag
     5521 ctgtcttgat tatccgcaaa gcacggaaat atgtggatct tcagcaagat gaagtgggga
     5581 aactaaatgg ttatatggat gaaaaaatca gcggacaacg tgtcatcatc acaaatggct
     5641 tacaagaaga aacgattgaa ggattcttag agcataatca aagtgttcgc gaagcaacct
     5701 tcaaaggaca agcttattct ggtctactat ttccgatgat gcaaggaatg tcactggtca
     5761 atacagcgat agttatcttt ttcggcggct ggttagcatt aaatggtgat ttggaacgtt
     5821 cggtggcatt aggcttagtt gtgacattcg tccagtattc acaacaatat taccaaccac
     5881 tgatgcagat ttcatctgga tacagcatga tccaattagc gattacaggg gcaagacgat
     5941 tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga aaacggtaaa gcatttgatg
     6001 gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg ttattctcca gaaaaactag
     6061 tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat ggttgctctt gttggtccaa
     6121 caggatcggg gaaaacgacg atcatgaatc tgctaaaccg tttttacgat gtcaatgatg
     6181 gttcagtagt catcgatgga atagatatcc gagattatga tcttgatgca ttacgtagac
     6241 atgtaggaat cgttttgcaa gaatctgtat tgttctccgg aacgatcaaa gacaatatca
     6301 cattcggcaa accggacgct tcagatgaag aagtcatcaa tgcagcaaaa caagcgaata
     6361 ttcatgactt catcatgacg ttagaaaaag gttatgatac agagatatcc gaagaaaata
     6421 acttattcag tacaggtcaa aagcagttga tcagtattgc tcgaacgatc atcacaaatc
     6481 catctttatt gattttggat gaagcaacaa gtaatgtaga cacggtaaca gaagcaagga
     6541 tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag ctttgtgatt gctcaccggc
     6601 tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg tgatggggaa gtcattgaag
     6661 aaggcagtca tcatgagtta ttagcagaaa atggatttta tgcggaactt tatcataatc
     6721 aatttgtatt tgaataaaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag
     6781 ccaatcaaaa acagcttctc atgtttttga tttggttgga cttattaccg cagatataag
     6841 cttttgagca ccgtgaataa agaggttatg aaatcaacgc tctaacctga gtattagaga
     6901 aaaaaacgca aaacatatca tttatgtttt gcgtttttta tttatctttt tttctcaata
     6961 caatataaaa taggcggctg gttcttttgg ttgataaacc gataattcag cacattatat
     7021 gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat ccagttcttt ttctccacct
     7081 tcatgaccat agtagacgac aaggatcaat cgtccgcgag gcacaagtcg tttcaatatt
     7141 tcttccatag ctgtgcgtgt agtctctggc atagtgatga ttgctttatc actttttggg
     7201 agatacccta gattgaaaat ccctgctttg agattttgtt gttcagctat cacttctcct
     7261 aacgtttcgt ggccttgtag aaacaatgag acacgttctt ctaactgacg ctgttctagc
     7321 ctttcacgag tagcatcgat tgcttgttgc tggatatcaa aactatagac atgaccagtt
     7381 tttccgatgt gttctgctag aaaaaccgta tcgtggccat tgcccatcgt tgcatctacg
     7441 acgtgatccc ctggttgcaa gatttcttgc agtaatgtat ggctgaaatg caaagcagtt
     7501 tgcaacatca gacgctcacc tttttccgta catcatattt tccttggaag ctatctctgc
     7561 gaagaagttc ttcatcaatt gcattcaaga cttcccattt tttcaagctc cacattggtc
     7621 caatcaagga atcccacggc gcatcccctg tcaaacgatg gatgatgatc tcttttggta
     7681 tcatttccag ctggtcgcaa atgacattga catagtcagg acggctcatt aattgaaggc
     7741 gaccttcatg atagtcccgc aacattctcg ttttgcgcat cagatgcatc aaatggagct
     7801 tgattccttg gatatctgaa tctaaaatgg tgcgtctgac attttctttc atcatttcta
     7861 aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac acggatatta tgtttgcgta
     7921 atttagccac gccatctaaa taagtttgat aatcatgtgc tcggttgatc agttttgaag
     7981 tttcttcaaa agttgtttgc aatccaagtt ctacccataa atacatccgc tcgttcagtt
     8041 cagcaaggta atcaactact tcatctggca gacaatccgg tcgtgtaccg actgataatc
     8101 ctacgacacc tggtaaattg actacttgct caaatcgttc tttgattatt tcaactggcg
     8161 catgggtgtt tgtaaaattt tggaaataaa cgatgtattg ttggacatga ggccattttt
     8221 gatgcatcat gtcgatttct ttctggaatt ggattggaag agggtcttca ggtgcaacga
     8281 tcatgtctcc cgaaccagat acactgcaaa aagtacaacc gccatgggca acagtcccat
     8341 ctcgattcgg acagtcaaat cctccatcga tcggcacctt gaaaattttc ccgccaaact
     8401 catttcgtaa agcgtaattc caagaatgat atcttttatt tccttctgaa taaggaaaag
     8461 tcattaaaag tccttctttc tgtaagagtt atctatttgt tttctttttt ctctatacac
     8521 tggataagtc aggcacagcc aagaaagact gagtaagtag ccgccgatga tatcagtagg
     8581 aaaatgaacg cccagataaa tgcggcttat cccgacactt actataataa acatcatcag
     8641 caattgaagc aatgtgcgta aggttctttt ttgaacaaaa gctggaagca gaaatagtaa
     8701 agaaccgtaa agaatcatac tgcctgctgc atgtccacta gggaaactgt aacttgtttc
     8761 aactactaag tgcgccaatg aaggacgctc tcgcatgaaa aatagtttga tcaccgggtt
     8821 gatgattcca gcaattacaa taaatccaga aacgaaccaa aatgtttctg cccttttttt
     8881 cttataccac atccataaag ccgcacaagc aaataaaatg atgacggtaa gaggattacc
     8941 gaatttagtc ataaagagtt ggtagctgtt cagatcagga taagaagctc tgacaatgcc
     9001 tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg aactttacga tccagcccaa
     9061 aatgaaaaac aggacaagaa agaagctacc agccacctga aatgatgttt tatatttcat
     9121 acaattcctc ctttgaagca atgacttatt atagcatttt tggtccagcg aaaaaaacgg
     9181 taaagagata aaaccaatgt atgattcctc tttgaataac caatattctt ataaaaaaaa
     9241 taactgtaat cggaaagaca ttttttggta atgcaaatga aatgttaggg aaataaaatc
     9301 agtgtttttc gaaatccttt taaacaagaa cgtagacgta ggaagaactt gaaaatgaac
     9361 agaagttgtt cattacggta aattacaata tgattacaaa aaacaacaaa gctcttaaat
     9421 aacttttaat aaatattaag agaagttata attattaata atagataaat tgaagttgga
     9481 ggatagaaga tggaaaacat tgcaagaaaa gaacggagac gtttagaaca gaccaaacgt
     9541 tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg cgatggtagg ctgttctgtc
     9601 gctgccccat tagtacagcc ggttcctgtt caagcggatg aaacacctgt tcagttcggc
     9661 gcaagaatca atacagcggc atttatcaca gaaattgcta cttatgctca accaatcgct
     9721 caagccaacg atctatatgc atcagtgatg atcgcacaag cagttgtaga aagcggttgg
     9781 ggcagtagtg cgttatctca agcaccttat tataatcttt ttgggataaa aggcagctat
     9841 caaggacaaa ccgtctacat ggatacgctt gaatttttaa ataatcaatg ggtaaataaa
     9901 aaggagcctt tccgacaata tccttctttt gctgaatctt tcaatgacaa tgcctatgta
     9961 cttagaaata cttcatttgg caacggttat tactatgccg gtacttggaa gagcaacacg
    10021 aaatcctata cagatgcgac agcttgtttg acagggcgtt atgcgactga tcctggttac
    10081 gcaggcaaat taaataatat catcgcaact tacggattga cccaatatga tacaccagct
    10141 agcggaaacg caggcggtgg agcgactaca ggtggcaata atactggaag tgcaggtagt
    10201 actggcggtt caagcaattc tggtaataca aactcaggtt cagcaacttc gaccacttat
    10261 actgtccaat ctggtgattc tgtttgggga atctcaaata aattcggtat cacaatggat
    10321 caattgatcc aatggaacaa catccaaaac aatttcattt atccaggaca aaaactgaca
    10381 atcaaaggta gccaggaaaa tggctcttcg acgaataatt ccggtaacaa taccaactct
    10441 tcaggaaatg ctggtacatc aaacggagga caaacaactg gtgcgaaata cactgttcaa
    10501 tctggtgatt ctgtttggaa gatctcaaat gatcacggaa ttacgatggc tcaattgatt
    10561 gaatggaaca atatcaagaa caatttcgtt tatccaggac aacagctaat cgtatcaaat
    10621 ggcggttctt ctaatgctgg agcaaatact ggctcaaata ctgggaatac aagtggctct
    10681 agttcgtcca gctcaagttc ttctaataca gcttcaggaa cgaaatatac agttaaagcc
    10741 ggagactctg tttggagcgt ctcaaataaa tacggaatta cgatggctca gctaatccaa
    10801 tggaacaata tccaaaataa ctttatctac ccaggacaac aactgactgt ttcaaacagt
    10861 ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa ccaatacaaa taacagcagt
    10921 tcagttaata ctggcagcag ttcgtctcaa ggcacttata ccgtgaaagc aggtgaatct
    10981 gtttggagcg tggcaaataa aaacggcatc acgatggatc agttaatcga atggaataat
    11041 atcaaaaaca acttcattta tccaggacag caattgatcg ttaaaggtgg aacttcggca
    11101 aatacaaata caggttcaac tacttctgca gcgaaaccaa atacaccgaa tacgtctgct
    11161 acgacaagta catcatccac tggtaatacg atgtataccg tgaaagcggg cgagtcagtt
    11221 tggagcgtag cgaacaaaca tcatatcacg atggatcagt taatcgaatg gaataatatc
    11281 aaaaataatt tcatctatcc aggacagcaa gtgattgtga agaaaggttc atctcaatca
    11341 acaaaccaac aatcagttac tggttcgaaa acctatactg tgaaatctgg agaatctgtt
    11401 tggagtgttg ctgacagtca tggaattaca atggctcaac taatcgaatg gaacaatatt
    11461 aagaacaact ttatttatcc aggtcaaacg ttgattgtag ctaaataaaa tagctgttcg
    11521 aagaaataac gattgaattc cacaaaaata tttgttacta taaggctact taaatatgac
    11581 aacacttgaa aaggaagtag taaaaagact gaagtttcag agagcgcatg gctggtgaga
    11641 atgtgcacgg atgcttttga actcgccttt gagttgccct tgctgaatac ccgagtaagc
    11701 aagcggcggt gacggccgtt acaacgttcg aggccttttc actaaggtaa aattaggtgg
    11761 taccgcgtag attacgtcct ataaaaagag caatcttttt ataggatttt tttatttctt
    11821 ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat aaacagattc tgccggtttt
    11881 ttcgttatat ttataataga aacaagtgtt ttcaatagaa caggaggaca gcaaatgagt
    11941 tacaatcata aagaaattga gaaaaaatgg caaaaatact gggcaaaaac gaatactttc
    12001 aacacccatg atgatcctga aaagccaaaa ttttacgcac tggatatgtt tccctatcca
    12061 tccggtcaag ggctacatgt agggcatcct gaaggttata cggcaacgga tatcctttca
    12121 cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa tggggtggga tgcattcggt
    12181 ctgcctgctg aacaatatgc gctagataca ggaaacgatc cagctgagtt tacccaaaaa
    12241 aatatcgaaa cattccgacg ccaaatcaat tctttaggat ttagttatga ctggaatcgc
    12301 gaagtgaata caactgatcc ggagtattat aaatggacac aatggatctt tacgaagctt
    12361 tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa actgggtacc tgaattagga
    12421 acagttattt caaatgaaga agtgatcgat gggaaaagtg aacgtggtgg ctatgatgta
    12481 gtcagaaaac caatgcgcca atggatgctg aaaatcactg cttatgctga tcgcttattg
    12541 gatgatctag aattagttga ttggccagaa agcattaaag aaatgcaaag aaattggatc
    12601 ggtcgttcag taggagcaaa tattgaattt aaagtggctg gtacggataa atcctatacc
    12661 gtatttacga cacgaccaga tactctattc ggtgcaactt actcggttct tgcacctgaa
    12721 ctagatcttg ttagagaaat tacgacccct gaacaaaaag ctgctgtaga agcttatatc
    12781 gaagagacag ctaaaaaatc agatctaaag agaacggatc ttgcaaaaga aaaaacagga
    12841 gtatttactg gtgcttatgc aatcaatcca gttaatggca aggaaatccc aatctggatc
    12901 gccgattatg tattatcttc atacggcaca ggagccatca tggctgttcc agcacatgat
    12961 gaacgtgact ttgaattcgc tcaaactttc ggactagaaa ttcttccagt tatcgaaggt
    13021 ggagatgttg aaaaagctgc ttacacagaa gatggtacgc acatcaactc tgaatttcta
    13081 gacggcatga acaaacaaga agcaatcgat aaaatgaatg aatggctaga agaaaatggc
    13141 gtagggaaaa aagaagtcag ctatcgctta cgtgactggc tattctcccg tcaacgttac
    13201 tggggtgaac caatcccaat catccattgg gaggacggaa cagtcacagc tgtcccagaa
    13261 gaagagctac cattacggtt gcctaagaca aaaaacatca agccaagcgg tactggagaa
    13321 tctccattag caaatatcga ggagtgggtc aatgtcgttg atcctgtaac aggtaaaaaa
    13381 ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa gctcatggta ttacctgcgt
    13441 tatatcgacc ctcataacaa aaaagaacta gcagattatg aaaagttgaa gcgttggcta
    13501 ccagttgaca tctacattgg tggagcagaa catgcggtac ttcatttact atatgcacga
    13561 ttctggcata aattcttata cgatatcggt gttgttccta caaaagaacc attccaaaaa
    13621 ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa aaatgtcaaa atctcgtggt
    13681 aatgtagtca atcctgatga tgtggtcgaa acttacggtg cagatacact gcgcatgtac
    13741 gaaatgttca tggggccatt agatgcttcg attgcttgga gcgaaaatgg cttagaagga
    13801 agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg atgagaacaa taaaatgcgt
    13861 gaccgtatca ctacattgaa tgatggcaag cttgataaag tgtatcatca aacagtgaaa
    13921 aaagtgacag aagactatga aaatcttcat ttcaatacag caatctccca attgatggtc
    13981 tttatcaatg aagcctataa agtggacgct ctgccttatg aatatatcga aggatttgtt
    14041 caactactcg caccgattgc tcctcacatg ggtgaagaac tatgggcaat ccttggaaat
    14101 gatggtggta tttcttacgc accatggcca acatacgatg aagcagcact tgttgaagac
    14161 gaagtagaag ttgttttcca agtaaacggt aaagttcgtg cgaaaagcaa tgttccccga
    14221 gacttaggaa aagatgagct agaaaaagca gcattagcca atgaaatagt gcaagaatat
    14281 attgaaggca aaacagtcag aaaagttatt gcggtaccaa acaagttagt caatatcgtt
    14341 gctaattaat tggctctttg tcaataagga ctgatgagtt gtgcaaaatc aaatctgggc
    14401 cagaatgaac ttcattctgt ctcagatttt ttgttatttc aaattgatta attgattgat
    14461 caaaaaggtt ttgattttca tgttctcaaa cacgttcaat gtctttttct tctgaatggt
    14521 cttgtaagag atctctgtaa tcatcaaatt tttgtctatt aatcttagta ttttttgatg
    14581 tataaactac ctgaaattga gagtacatac caaattcata aattttttat tagtgggact
    14641 agtgattgta tggattttga cgcataatga atgaagtatg agaaaaggag agacaaatgg
    14701 atcaagaact atttattatc gaagtattat cagctaaaca aaaaattatc gagaaagcga
    14761 aaaaaagagc gaaaagaata atgttcaagc ttttaagctt agtatttttc tctggtttgc
    14821 tttttttcgg agctgtatcg ttcctatatc gccatatgag tgcagtagtc cgttcagctg
    14881 aagggatgca aaaattaaat aatttgtatc acttttcgtg gggagttggc ggaataacaa
    14941 ttgttgtgct tattgttttt cttctactta tttggctccg ttaccatcaa ttgtcgcgtc
    15001 atttcgatca atggcttagt caaagagcac aacagttttt acataataaa ccaacttctc
    15061 aagatgatga ctatctttat tttgagaaga cttcgacgag tcaagctttt cggatacaaa
    15121 aaagtgattg ccagttattg ctgagatcac ttgatgattt acctatctac ttaggcgaaa
    15181 gcaaatcaaa attcggaatg aacacattcc atattttttt actagaaaac gaacccagat
    15241 gggaaaaaag ccagtcttcg cctggtggat acctgacaac taagatgagt acattagtca
    15301 ctggtttttt cctcattctt ttagctggaa caatctatta tgggagatac actcagccaa
    15361 aaatgtcttc tcatataaat gctgcttctc acatgacaat gaactcaact aacgatacgc
    15421 atatacaaaa gacaaatgaa gggttgttag ctgatcctaa gcaaaaggta aatttgacaa
    15481 cacaaaagat aaatgatctc aaacttgata cagagacgca ctctctctat atgacaacag
    15541 atagtggtgc taattgggta tttgtgccga ttgattcgca atggttgcgt tttggcgaat
    15601 acaccctgac aacggggctg attcctgaag gttattggat ggacaacaca tatgatattt
    15661 ccgccgattt ctcatggttt atctattctt ctggggatga tgtacgtctg ctgacttcaa
    15721 gagataatgg taaaacatgg caaacgtcta ttatcaccca atcagttcag ccgatacgtt
    15781 atcgaaaagc ggtattttat ggcaacgatg gtctatttat ctgtacatca gccagtggga
    15841 tgtctgcaga aactttgtac atttacacta ctatggatgg cggaatcact tggataaaaa
    15901 gtggaagtac aatgatcaat cagccagtac aaaatgtcag cttcgttaca acttctttag
    15961 gttttgtttc gacacgtgaa agtttgtttt atacaaacaa cggaggacgc tcattcaaag
    16021 aatcagttct gtcgattccg gaaaactatc agacaggggg gatagatctt tttcaatcac
    16081 caaatgaagt tactcaaata agcgcgaata cgttagagac aaaattttat ttgttgaaaa
    16141 aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt ccgttcaact gataacgggg
    16201 aaacatggca attcgttcag caactatcgc aagtagaaca aggagattaa agaagagaga
    16261 ttgtgagcct gacgttcagc accgagtcac aagaaacaaa ttcaaaaaac agcttctcat
    16321 atttgttgaa tagtgtaact cgtgattcgg agttggcaag agcaacaaga agcgattttg
    16381 aagtatagct tctcgtattt tacaagatcg tgtcttattg ccgataggtt gtctcttgaa
    16441 caccgtttat tcgtaaaaag ggattgtgac aagtcttttg tcactactta ccgaacgaaa
    16501 ctgaaggagc tatccaaaaa gtatcattag tattattctt tgtgtttcta gtggcaccac
    16561 tagtacattt aaaaaagcta tcgatcatgg actaatcata catgatcgat agctttttag
    16621 ataatgtttg ttacgctatc tgaaattgac tattataaag actggcataa agcgttggtg
    16681 attggagtag ttcagaatgt gttccttttt cgacgatatc gccattcttc attacaagga
    16741 tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat cacaaaactt gtcctatctt
    16801 ttgtcacagc ttccattgct tgctgaatca atgcttctgt tcgtgtatct acgctagaag
    16861 ttgcttcatc taaaataacg acaggaggat tcgctagaat gactcgagca attgtcaata
    16921 attgttgctg tccttgagat aacgtgccgt tttcgctaga aatgatcgta tcatatccgt
    16981 ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc agccgcttcg ataatttgtt
    17041 ctcttgtggc atccattttc ccgtaggcga tattatcagc taccgttcct tcaaacagcc
    17101 aggtattttg taaaaccatc ccaaaagaac gacgtagttc tccacgtgaa agcttggtaa
    17161 tatcaagacc gtcgaattcg atctttcctt tattcagttc ataaaaacgc atcagcagat
    17221 tgactaaagt cgtttttcct gcaccagttg gaccaacgat cgctaccgtt tgttttggct
    17281 ttactgaaaa atcgacttga ttcatcaata ttttatctga tgtgtaacca aactgtacat
    17341 ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct tgcatctgga aggtctggaa
    17401 tttcatctgt ttcatctaat atctcaaata tcctatcgat tgctgccatt gcggattgga
    17461 tcgagttgat cacataagaa gcggtagaaa ttggttcaga aatctgattg atgtactgaa
    17521 gataagcttg taagaagccg atcgtcacac cacctgatag aacaagcata gcaccgaata
    17581 ccgcacttat gatgaaggcg agttgattaa tgaaacgaat ggccggatag atagcacagt
    17641 tcataaattg agcacgttta aaagcgttgt attgcttttt atttacttcg tgtatagcag
    17701 cttctgcatg agcttgctga ttgaatgttt tgaccactag attaccagct aagatttctt
    17761 ccattcgatt atttaaaaca cccagttcat actgattctg ttcagaaatc tgtttgtttt
    17821 tgttcgcaat cttagttgta acaattgaac tgccaacgat cagcaataag acgagcaaag
    17881 taagcttgct ttcgatataa aacagcatca gaccagcagc agctattgtt acgacagaag
    17941 taaaaaattg attgatacct gtcaataaca cttgagaaag ttgattgaga ccggtagttg
    18001 tccgactaat gatgtcacct acttgatgat tgtcaaaaaa cgacatcggt aatgttttga
    18061 atttttttgt gatttctttt cgcaccctca atgtgatctt ttcgcttaac gctgccattg
    18121 tccgttcctg aataaatgaa gtgatgctgc ttaagactga acagccgatc agcaataaga
    18181 cagggaacaa caatgcttgt ttgatatcac tgaaagaaag ctgtccaata cctgtttttc
    18241 ttatttctga taagagatca tcgatcccga ttcccatgat caagggcatg gctacgacta
    18301 ataggttccc gaataaactg catagaaaca atccgtaaaa atatttatgt tcaggtttga
    18361 tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt ttttttcttt tccatcgtat
    18421 cccttctttc tatattcctt gtgattttgc aaactcctga taagtttggc aattagacag
    18481 tagttcttca tgagtacctt gtccaacgat ttttccttca tctaaaacaa tgatgttatc
    18541 tgcttgttgg attgtactga ggcgttgagc aacaatcacg agagttttct ccgacatgtg
    18601 tttatgaagg gctgctctta atgctgcatc tgttttatag tctaaagcgg agaaactatc
    18661 atcgaataca tagatctctg ctggtttgat caacgctcga gcaatacata tccgctgttt
    18721 ctggccccct gaataattag aaccgccttg agcaacaaag ctgtccaatc cgtcaggaag
    18781 ggtgtccact agttcttcta attgagcgat cttgatcgct ttagacaact cttctgttga
    18841 ggcatattca tttcccatct gcaagttgct taaaattgtc ccgctgaata agaaagcctt
    18901 ctgagggaca taactgatgt gggagcggat ctgttcttga gtcatttgag taagaggaat
    18961 accaccaagg gttacttttc ctatcgtagg gtcactaagt ctcaagagaa gtttcgccaa
    19021 actacttttt ccagatccgg tcccgccaac gatcgctgtt gtttttcctt taggtattgt
    19081 gaaatcaata tcttctagaa ctggatcaga gatttcagta tatttaaatg tgacatgttc
    19141 aaaagcagct gcatttgtct gatcaaaggt tggttcagct actgttttga tcgtctcttc
    19201 ctcaatttcg atctctgctt gcagtatttc ttctatccgt cgtaatgagg caaacgattt
    19261 agggataatg accaatacca tagctgcgat catcaagtaa gcaagtgtca gcatagagta
    19321 ttcgactaca gcagtcacga caccgatttg taatgctcct tctgctacaa gatttccgcc
    19381 aaaccacaaa atagaagcat aaacgattcc catgagcaaa aaagcaattg gagtgaccca
    19441 agcaaatatt cggttcactt tgatcatttg attagcatag tcagcaaagc tcttattcgt
    19501 tcgcttttct tcatatgatt gattatcaaa ggcacggatc atattaatcc cagtaaagaa
    19561 ttctctcaat gtgatggtga tccgatccat ttttggctgg atactcaaag ataaaggtgc
    19621 ccctcgtttc atcagtcgat aaacaatcca tacataaaaa acaacagaga tcaatgtaac
    19681 ccatgttagt aatagagaat aacgaaacat catgagaatg gtgaaaacgc aaataaccgg
    19741 tgctggtaag atcatttggc aaaataaaac aatcatttgc tggacattat cgacatcatt
    19801 cgtcattctt gtcaaaagag atccagaact aaaacgactg atatccttca ttgacagctg
    19861 ttggaattta cggaaatatt tctcccgcgt ttggaatcca aacctagcgg aagccaatgc
    19921 tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga aacgctacaa gcagcatttc
    19981 aactccaatt ttttttataa ctgaggcttc gccacttgct atacctgtgt cgatcagctt
    20041 tgctactaat tttggtacac ctaatgtacc ggcgatttct aaacagatca tcacaaaggt
    20101 aaggagagcg agtcctttgt ttttctgtaa gaatgttttt aagatagaca tattattcct
    20161 ccttttttcc tgatactttt cgattctata ccttcaagta acttgaaggt caatagaaaa
    20221 aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa tgtatacgat tggccaagta
    20281 tcggaactat ttgatttgcc aaaatcaacg atccgctatt gggatgaaca aggattaatc
    20341 cgttcaagta gacaagaaga gaatgattat cgattgtttg atatcgatga tatttttatg
    20401 ctttatgata tcgtttttta tagaaaatta gatataccga tcaaacagat gaagaattta
    20461 tatggaaaaa cattgacgga gttatatgag acacttgatg aaacagaaag aagaatccat
    20521 aaagaactgg tggtgatgaa acaaaaacaa aaagagataa gggagcaaaa aaaacagttg
    20581 aaattgatga tcgatacaaa cgaggaagaa tttcctgtag aagagatccc ttttgactgt
    20641 atgatctcaa cggagtttga agatattgtg gaaatcaaaa aatttctacc taactattca
    20701 agctttggga tgatgagtat gccagcttcc agttcacaga cgatgtatgg attttttatc
    20761 gaccctagtg aagtacattt atttactcaa gacgtgattt gggagaaaaa agatacggct
    20821 gtttatcgtc gattcttatt gaaatccgag atgaaccatg cggaacgtaa taatatttta
    20881 gaaatcagag aaagaatgta tgaaaaagga tggaaaacag gagaagtgat cggccagtat
    20941 ctattgacga atacggatga aaacaatatc agaactgaat attatcatgc atggatagaa
    21001 atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa tgatggttgt tttagttata
    21061 aagaaaaatt ttctggttcg attacgggtt taatgtgggg gattattagt cgtggataga
    21121 tagaatttta gttttttaga ggaaatatgt ctttaaaaat ggaatggttg caattcttag
    21181 cgatatatac gatgtaattg tcctcttaaa aaagtatgga aaaataattg tattgggtgt
    21241 tatgtataat tcatggtgaa ctgagactaa tgagaagaaa aattcatatt gttatgagtg
    21301 agattgtatc agatgagaat gataaattaa ataggtgcat aaaagcatta cacttaaaat
    21361 acattgttgc ttacattatt acaatatgtt aataaggcaa aaataaatag atggattgaa
    21421 taaacatatt ttttaatcaa atagtcccta ctgtgacttt cctcagctat cggtgatact
    21481 accgatagca tttttttgct t
//
LOCUS       DAYYAO010000048        21261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000048 DAYYAO010000000
VERSION     DAYYAO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.050"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..679)
                     /locus_tag="ACHARI_001696"
     CDS             complement(68..679)
                     /locus_tag="ACHARI_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8442745.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(802..927)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1009..1725)
                     /locus_tag="ACHARI_001697"
     CDS             complement(1009..1725)
                     /locus_tag="ACHARI_001697"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8442746.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1731..2300)
                     /gene="scpB"
                     /locus_tag="ACHARI_001698"
     CDS             complement(1731..2300)
                     /gene="scpB"
                     /locus_tag="ACHARI_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8442747.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2284..3054)
                     /locus_tag="ACHARI_001699"
     CDS             complement(2284..3054)
                     /locus_tag="ACHARI_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8442748.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3051..3422)
                     /locus_tag="ACHARI_001700"
     CDS             complement(3051..3422)
                     /locus_tag="ACHARI_001700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8442749.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3441..4328)
                     /gene="xerD"
                     /locus_tag="ACHARI_001701"
     CDS             complement(3441..4328)
                     /gene="xerD"
                     /locus_tag="ACHARI_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8442750.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4424..4897)
                     /locus_tag="ACHARI_001702"
     CDS             complement(4424..4897)
                     /locus_tag="ACHARI_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8442751.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5002..5865)
                     /locus_tag="ACHARI_001703"
     CDS             complement(5002..5865)
                     /locus_tag="ACHARI_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8442752.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6061..8160)
                     /locus_tag="ACHARI_001704"
     CDS             complement(6061..8160)
                     /locus_tag="ACHARI_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8442753.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8380..9489)
                     /gene="rpoD"
                     /locus_tag="ACHARI_001705"
     CDS             complement(8380..9489)
                     /gene="rpoD"
                     /locus_tag="ACHARI_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8442754.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9513..11354)
                     /gene="dnaG"
                     /locus_tag="ACHARI_001706"
     CDS             complement(9513..11354)
                     /gene="dnaG"
                     /locus_tag="ACHARI_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8442755.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11536..13851)
                     /locus_tag="ACHARI_001707"
     CDS             complement(11536..13851)
                     /locus_tag="ACHARI_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8442756.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14036..14896)
                     /locus_tag="ACHARI_001708"
     CDS             complement(14036..14896)
                     /locus_tag="ACHARI_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8442757.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14955..15587)
                     /locus_tag="ACHARI_001709"
                     /pseudo
     CDS             complement(14955..15587)
                     /locus_tag="ACHARI_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15754..16515
                     /locus_tag="ACHARI_001710"
     CDS             15754..16515
                     /locus_tag="ACHARI_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8442758.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16541..19132
                     /gene="mprF"
                     /locus_tag="ACHARI_001711"
     CDS             16541..19132
                     /gene="mprF"
                     /locus_tag="ACHARI_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8442759.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19320..20507)
                     /gene="tuf"
                     /locus_tag="ACHARI_001712"
     CDS             complement(19320..20507)
                     /gene="tuf"
                     /locus_tag="ACHARI_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8442760.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20875..21231
                     /locus_tag="ACHARI_001713"
     CDS             20875..21231
                     /locus_tag="ACHARI_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8442761.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         5821 a         4461 c         3723 g         7256 t
ORIGIN      
        1 cattattgca tgtcttgtat ttttttccaa aaaaaaagac caagaaattt cttgatcttt
       61 ttgatattta tttgactgct gtatgacgtt gttcgaactg atgttgtttt cgacttaacc
      121 atggaagaag ttgcgcatgc agcactaaaa agacagcact cacgatgaat cctttgatca
      181 agttgaaagg aacgatccca atcagtacat agtttgctaa agacatgccc agcatctgtg
      241 ttgcgttcag ccctaaaaac attaagtaaa gaggcgtaat aacgaaataa tttgcgatac
      301 tcataaaaat agttagagcg atggttcctg taatgacacc taaaaatttg tttcttcctt
      361 tatgctgttt ctttacacta aagaaataat aaatcgggaa tgtaaagatc gttgttgata
      421 agaaactagc agtgtctcct accaaattat ccggagaaaa acctgtagtc agcagatgta
      481 atactgaacg caagaacgct gttgcaactc cggccgctgg accgaataag aacatgctga
      541 tcaataccga gatatcacta aaatcgatct tcaaaaaact aaaagccggc atgatcggaa
      601 aagcaaaata ctgtaaaact aatgcgatcg cagcaaacat cgctactgca accattttct
      661 gtacacgggt gttcttcatt tgaaatcctc ctgttaggac tcctcttcag cgaacttttt
      721 gacgtttctc tccaaatgaa aaaactctat tttccaggga aaatagagtt agagttattt
      781 gatcaagcgt cgtcaaataa gctctatctt ctttatccag actatactgt cggtatcgga
      841 atttcaccga ttcagctatt tttttgcaaa ataggtcgtg gactataacc accggtcggg
      901 aatttcaccc tgcccctgaa gacgaacctt atataatttt tgtttacata ttcattatac
      961 atatctattc aaaaaacgca acatctaaca cttataaaaa gtaagtgttc attttgagtg
     1021 gttcagcaaa ctgctgactt cttttttgtt cagttcacgg taatccccag gacgtaggcc
     1081 ttgtaaagtc agatctccgt atttttcacg ttttagtttt tgcacaggaa gaccaactgc
     1141 ttgaagcata tttttcactt gatgattacg tccttcgtgg atcgtcaatt ccacgatact
     1201 gtggtttgtc ttaggatcca ctgataaaat ttggaattta gctggagatg tttttcgtcc
     1261 ttcgatcttt attccttttg tcaaaggtgc aagcatcgat tttgtagcga tcccttttac
     1321 ctttgcaacg taaactttat ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc
     1381 tccatcattt gtcagtagca agatccccga agtatcgtaa tccagtcgac ctacaggata
     1441 gatacgttct tttaccatcg gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc
     1501 aacagcagaa ataactcctt taggtttgta caataaatag taaccgtatt cttcttgata
     1561 aattgggact tcatccactt ccacagtgtc atgtttccca acttttatcc ctaattccct
     1621 tacaacttgt ccgtttactt ttacacgtcc tgcaaggatg aattcttctg cttttcgacg
     1681 agaagtgatg ccagcgtggg caattacttt ctgcaatctc tccattacaa ttattctcct
     1741 ttattcattc catcaaagaa tagatctggt gacacgtctt cttcaagtga aatttccatt
     1801 tcttgtgtat caggcagttc ttctaatgat ttcaaaccaa aataatctaa gaagtagcca
     1861 gttgttccat ataatatcgg ccgacctggt ccttctactc tacctttttc ttcaatcagt
     1921 ttatgggcag ctagacgctg taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt
     1981 tcagctcttg tgattggctg tttataagcg atgattgcta aggtttcgac ggcggcttga
     2041 gataatgtgt ttgatgtccc ttgcgcatat cgcttaagta agggagccag ctctttttta
     2101 gtcgttaata cataatggct ttccgtttca aaaatatgca aagctgagtg cgagttttga
     2161 gtgtaagatt cttttaattc tttcactaaa cttgtgactt ctgcttcgct gagatttaac
     2221 agtccgctta attcttcatt actgatccct tcctcgccaa caataaaaag aagggcttca
     2281 agttcactta atttcgtcat gttcggtatc tcctactgct ggataaagca taattgattc
     2341 atagttttct ttttgttgcg caatgatctg cccttttttc atcaattcta gcaaagccat
     2401 aaaagtagtg acaacttcac ttttagaagg tgactcaaaa aaagaatcga aagtacagcc
     2461 atgcgcattt ctttttatac gctcttcgat aaataccatt cgttcttcga tcgttgtgtc
     2521 atcgggcgtg atcgtttttt ccactttttg agaaaatttc ttacgttcta agattttctg
     2581 catggcacgg atcaaatcga tttttttgac ttgtcctcgc ttcaacggct tttcagcatc
     2641 cgctaaccca tcgatgttca taggttcttt tgtgtaataa agacttcgtt cctctgcttt
     2701 ttctgtcagt ctttcagctg catatttata tttacggtat tctaaaagct gagtgaccaa
     2761 ttcttctcga ggatcttctt cccataattc ttcatcgtct gtgacgaatt cttggacagg
     2821 aagcaatgtc ttgcttttga ttgccatcaa tgtagcagcc atcactaagt attctcctgc
     2881 aacagctagc tcgagtgttt tcattgtatg gatataattc atatattgct ccgtgatcgt
     2941 agcgatcggt atatcatata tatcgatttc catttgctga atcagatgaa gcagcagatc
     3001 gagaggaccc tcgaatacat ctaatttaat attgatctcc gtcaactcat tcactctttc
     3061 ttctttttct ttgctagcca ttttccaatg taaggctctg tgatttcagt tgcacggatg
     3121 gagacatccg ggtacttttc ctgtaattca tctaacattt gaaagcccat attctcaccg
     3181 cgataagacg gattcaaaga aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa
     3241 ataccaataa tcccttgaat gttggttgaa ccttctggcg taaagaaaaa aagttcataa
     3301 tcgtcttcag attcgtacgt atcaatctcc ttcaacaaag aagttttacc tgtcagtttc
     3361 ttatggaaac tcaatagtcc catagcaatt ttacggtttt ctttccggta atgtgttaac
     3421 atcttctcac cttcctatga ctaagccctt ggaaaatgct gcttataaac atccgtcatt
     3481 cttttcttcg tgatatgtgt ataaatctga gtcgtagaga tatccgcgtg acctaacaat
     3541 tcctgtactg tccgcaaatc agctccattt tctagcagat gtgtggcaaa gctatgccgc
     3601 agcgtatgtg gtgttacttc tttgtaaatc ccagcctctc gtactaactg cttcagattt
     3661 ttccagattc cttgccgaga aagacctttc ccatgattat tgacaaatac atgcatttca
     3721 gatgcgtcct ttacaagtaa tggacgtgct tcgtctaagt agcgttccag ccattggatc
     3781 gcataatcac ctaaaggaat gatccgctct ttatctcctt ttccaactgt ctgtaccagt
     3841 ccaagagaaa gatgaagatc tcccaatttc aatccaatca attcactgac gcgcatccca
     3901 gtagcataca tgacttccaa gattgcacga tctcttatcc ctaacgtttt tgtcgtatca
     3961 ggtgcttcaa tcagcttttc tacttctgtc agagtcaatg tacttggtaa tttctgtgct
     4021 ttcttcggcg tatcgatatg ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa
     4081 aactgatgga atcttctgag acttgagatc atccgagtaa cagttgctgt cgcttttttt
     4141 tcttcatgca acttctccag aaaacggatg actaaaaagc gatcgacatc ctgccatgaa
     4201 gcaattttct gctcctgtag aaaatgcaag tactgttcca aatcacgttc atagctttgt
     4261 cgtgtattgg cggacaatcc ccgttcaata ttcaagtagt ggagataatc gttgatttgc
     4321 tctttcataa taatccccct catgcctctc tatcatatca gataccgctt cattctgata
     4381 gtctttttcc ataatctgag caaagaaaaa gccgtctaga cactcagacg gactggtttc
     4441 ttttttcttt ttcatagcat tcctgacaga ttccatggaa cgttagccga tgatctttta
     4501 ctaaaaaatg ataacgttct tctactactt gctccacttc aagtaaaaga tcctcttcta
     4561 cttcctcgat cgtgccgcag ttcaagcata gtaaatgatg atggaaatgt ttggcgcctt
     4621 cttttctcaa atcataacgg gctaatccat cattaaaact tactttatcg acaattttca
     4681 attctgtcaa gatttcaagg gttcgataaa cagtagcaag tccaatttcc ggactctttt
     4741 ttttcactaa aaagaagatt tcttctgcag ataaatgctc tttctcattt tccaaaagca
     4801 caagcagagt agcttctctt tgcggtgtca gcttaaaccc cgcttcatgt agctgctgtt
     4861 ttgttttttt catcgccgaa atagcattca tgaccattta ctcaacccca acaatttata
     4921 ataattacaa ataaaaaaag atataacact atgttaatta aaattattat aagctagtta
     4981 agaaaaacat gcaagggatt ctcaattaga ttttttgact agttttgtca caccgttttc
     5041 ttgtgtaatc aatccttgtt tcaaaagatt acctaatgcg cgcttaaatg ctcctttgct
     5101 gataccaaaa gctttcatga tttcttctgg atccgattta tctgtaaagt tgatttgctg
     5161 atctgcagaa cgttctaaaa tggttaaaat catttgtgca tcatctgata tagcttcata
     5221 tgctcttggc ttcaatgaaa gattcagtac accgtctggt cggacaccaa tcactcgacc
     5281 ttttatctgc tcgcctagac gaggttcatt atatcgttca gaaggatgga taaacccaag
     5341 atagaaatct tccgttaaaa cgtacgtacc tgttaatttc aagcggtatg ctgttcctat
     5401 cacatctttg tttttcattt cttctgttcc atatttgctt aaagatttga atattttttc
     5461 atctgctaaa ctgccccaaa tacggtcttt tgcatcaact ctaagggaaa tcatcaagcg
     5521 atcccctttt ttaggccata gctctgtcat tgtcggcaat tcatcaagag atacgacaaa
     5581 gtctttatct ggaagaccga tatcgacaaa cgcacctaaa tcttttcgtg tagcagtaac
     5641 gattccaaaa gcgtaatgac cgatacggct tttaggaatt tgtgtagtga tccgattttc
     5701 atgtttttga ttttggtaac cgaatccttc aactgcttca ccgattttgt gttcaccttc
     5761 tgatttgctt aagccaaagg tttgtccgtt tttttggaca aagtagtttt tttcattttc
     5821 atcaataata atacctgtaa atatagttgc taataaatcg ttcatttttt tctccctcta
     5881 tagtgaaagg ttttcttaat acatttcttt gctatcataa cataatgtta tctacttgaa
     5941 tagctgagcc ttggtttgga aaggtgaaaa aaggagtatg acaacagttg tgtcatactc
     6001 ccctttttcg aataaacggt gttaaacggc aggctcacag cctctttcat ttgcctaaaa
     6061 ttaattgctc ccatttcctc cgaaccatcc agttttacag ttgatggtta cgatgtaaaa
     6121 tggcaaagca ctttctccaa tgcctttcat caatgcagat tttgttgaag tggcatatag
     6181 attataatag ccttccccat tttcgattct ggctttttct aatctgtaac ctccttcaac
     6241 gatatatcca cgttggatac atgtagcaat cactttgctt tggacacctg gtgcccacgt
     6301 ccatgctgga ttagatgcat cggcaatatc acttgctctg gcagatttga ttggctgatt
     6361 cgctgagttt gaagaggaag gggtcgagga tgccgcttct tgctgtgctg cttgtgcgac
     6421 cgcagcttgt tgagcggctt gagctgcagc tgctgcttga gcggcttgtt ttgcttgttc
     6481 ttcagctgct tttttcgctt cttctgcttt tttcttttcc tcttcttctt ttttctcttt
     6541 atcagtgagc atcttatcta cttgtttcaa ttgttcattc aactgagcag atagatctgt
     6601 atttttcact ttttctactg cttcttttgc cgtcttgtat tggtcgcgtg ttgctgaatc
     6661 cactactttt ccgtctttaa agactatagc tgttttttct ttagctgttt gcaattcagc
     6721 atattgattt ttagcttctg tttgtgcttg ttgaatcaat tttccaaaat ctgtgttatc
     6781 cgtttcgatt gcttggatct ctttatctgc tttcaacttg atgtcatctg ctagatgatc
     6841 atcgacgatg gcaggtgcta tgaaccactc attcactttt tggatatctc ggatcttaga
     6901 ttggatatct tggtaagttg tttctaactc tgaatatcct ttttcattct taagactgga
     6961 taaatcattt tttagttttg ataaatcttg atgaatcata tctgcacgaa taaattgatg
     7021 gtgatcatct gtataaaagg cagcaagttt ctcatgaagt tcttccattt gttgctgtct
     7081 ttttgcttct gctgctactt cggcttctac tttttgatga tggtcataat aagcccagct
     7141 tcctccaccg agtagaagta ctgctgctaa aacgagataa ggtacttttt ttgatttttt
     7201 cttttgcgct ttatctggat ctgttttttc taagtttgat tcttgatgtg ttttttcagt
     7261 atctggcacg attgttattg atttcgcttc tgcttcaaac agcttaggct ggttctcttc
     7321 tttttctatt tttgcttcaa gatcagtacc ctctttgctt acagattgat tttcagcatc
     7381 ggattcacta tttatttcat cgggctctgt tgactttttg tctgtgtttt cgagtatttc
     7441 ttttttctct gcttccggca gtttattcgt tttttccttt tctaattgtt tttcagtaaa
     7501 tacttcagtt ttttctgtga tatccgtttt tacgtctttt atttttactg tttctttacc
     7561 tgtggcgttt tctctatcag agtctttttc agtttcttgt tccttttctg gctctacttt
     7621 gttttcctgt gcaatttttt cactgatcat tttgagcagc tctttcgcgt gctcttcttt
     7681 tgtatcttca cgatgtgctt tgatataagc ggatagtatc gaattttctt cctcattatc
     7741 gacgatttct ttcagttcag ctactcttct tttttgtttt gattcaagcg tttcttcttg
     7801 attctttcct gattcttccg aagtctcatg aacagttgct ttcttctgtt catttttctc
     7861 tatattagtt acttgatcgt gagcaggtgg ttcttcgtct tctggttctg ctttttcgaa
     7921 taattccatt acagattcta gaggtaaatc tttcaattcc gaccattcga ttggatcgtt
     7981 taattctgta tcaggaaaga ctgcatcttc agttgattta tcctgcatag ctgcatctgt
     8041 atttgtttct tcaattgatt cttttatttg ctgatcttct gcagttattt gatgaccgca
     8101 gtctggacag atagtattct gctccatttt tttcgatccg caatttggac attttttcat
     8161 cacttagcga tcggctcctt tactaggttt acgctaccat ttaatctatc atattctata
     8221 cgtcacgcca atagcttatt tttaggattt acttaaaaat aatgaatgaa tttccttcct
     8281 atagaaaaaa aagcttgaga ctgcgtagtc tcaagctttt tttcttttaa aataaaccaa
     8341 acagttttac tgatttctct tctggtcaat agtccacgct tattctaaga aatcttttaa
     8401 ttgttttgaa cgagaaggat ggcgcaattt tcttaatgct ttagcctcga tctgacgaat
     8461 acgttcccga gtaacaccaa atactttccc tacttcttcc aatgttctcg tccggccatc
     8521 atctaaacca aaacgtaagc gaaggacatt ctcttcccga tcagtcaatg tatctagtac
     8581 atcttccaat tgttctttta gtaactcata agctgcgtgt tctgctggac ttgttgcttc
     8641 ctggtcttct atgaaatcac ctaagtgaga gtcatcttct tcaccgattg gcgtttcaag
     8701 ggaaacaggt tcttgcgcga ttttcaagat ttctcggact ttttctgttg gaagatccat
     8761 ttccgcaccg atttcttctg gcgtaggttc tcttccaagg tcttgcagca attgacgttg
     8821 gatacgtatc aatttattaa ttgtttcaac catatgaact ggaatacgga tcgtacgtgc
     8881 ttggtcagcg attgctcgag tgatggcttg acgaatccac catgtagcat aggttgaaaa
     8941 cttaaatcct ttgcggtagt caaatttttc aactgccttc atcagtccca tgtttccttc
     9001 ttggatcaag tcaaggaact gcatgcctcg tcctacataa cgtttagcga tacttactac
     9061 taaacgaagg tttgcttctg ccagacgctg tttcgcttct tggtcgcctt cttcgatttt
     9121 caatgctaac gctacttctt cttccgcagt taaaagggag acacggccaa tttctttcag
     9181 atacatacga accgggtcat tgatttttac accggttggc gcagaaagat cttctgtttt
     9241 tgcttgttca gcttttttct cagcactctt caagctgtga attgatggat caccattttc
     9301 atccaccaca ctgatacctg cgtcttcaat cttttggatc aatttgtcca tttcatctgc
     9361 attcaaagtg aatggtgtag caagctgatt ggataaatca tcatatacaa cttgtcgttt
     9421 gggtttgttt tctttgatga aagctgctac tgctgcttca tacgtttttt tatctgttcc
     9481 atttgccata aaagaaggcc ccccttgagt agctatttgg cttgttttaa ctgtttcgtt
     9541 aaattgatga tttcaatcgt caattcaagc tcaagctgtt tgtttccagt tcgtctggct
     9601 tcttgctgca tttgtttttt taatgttatt tcatcagcta agccagattt gctgatcaca
     9661 tctagcaagt cttggatttc tcgtggcgaa ctttcttctg ccaccggtaa atattcgatt
     9721 tccaccacca accgccttaa attttcatct tgcaaaaagt ctaaaaactc tgcaaggttg
     9781 aattcataat gttgtgagac atatgcatcg aataaaaaat aaacttctgc atatgcatca
     9841 tgaataaact gaaattctcg ttcttttaaa atattacgga cagaaacttc tttaaatgca
     9901 cgatataata gaatctgctc tgccttttct acttgcgtca aaggtctctt tttccgtgca
     9961 gtttcttgtc tcgtaacctg agtgtgttga ctttcctgag gttcttgacg attttgctga
    10021 tttgtccttc gaagatcgcg cagttgtttt tgtatcgtct ctctgccgat ctgaaactct
    10081 gttgacagtt gagatagata catatcttgt tcgattggtg aaacaactcg gctaagttca
    10141 actagtaatt cttcgaggta ttcgatcttg tctttttcat tatctaaatt tttcccttgt
    10201 tttaaataac gtgatttaaa agtaaagact gtttcgcgcc catgagccac taattcttgg
    10261 aaagatgctt ctccatactt acggagatat tcatcaggat caagcttttc tggaacgact
    10321 acgacagaaa gtgctaatcg actgttttgc cgaagcaatt caatccctcg attggttgct
    10381 tcaaaacctg cgttgtctcc gtcatagcaa aagaccaatt ctttcgtcag cttttccatt
    10441 tgttggatct gttgagctgt tagacttgtc cccatagaag ctaatccagt tttgatacca
    10501 gcctgccatg cagcaatcac atccataaac ccttcgaaca acaaaatttc tccgtttttc
    10561 cggatatcgc ttcttgcttt atcaagatta aataaaactt gtcttttatt gaacagttct
    10621 gtttctggac tattcaagta tttgggctga tctttttcct gccctttttc aggtgcaagc
    10681 cagcgtcctg aaaaaccgat cgtctggcct tgagaattac gaagtggaaa catcactctt
    10741 tgataaaaac gatcagacat acggccatca tcatgcgtga caaacaatcc agagcgttct
    10801 aacaattcat ttgaaattga ttcattcttg aatacttgct gaagaaaaga acgctcatta
    10861 ggtgcaaaac ctatgttgaa ttcttcaatt aattctaaag acaacccacg actttgcaaa
    10921 tattctaaag ctggctgtcc agcttttgta tgcaatagca tgtgatgata gatttccgct
    10981 gctttttcat gagcagcaat cagtttcccc gtagcagatt caggattttg gacagctata
    11041 gtacgctgac gccattgatc ttctaacggt atctgttcga agtctgccac ttttaccact
    11101 gcttctggaa aacttagacc ttctagttct tgaagaaaag taaacacgtt tccgccttta
    11161 ccacaaccaa agcaatgaaa aatctgcttg tcttctgcaa cagaaaaaga cggtgtacgt
    11221 tcttcatgaa aaggacacag ccccaagtaa tttttaccag actttttcaa ctggacatat
    11281 tgtccaatca cctcaacgat attcgtttga ctgcgtactg tttcgataac ctcttgagga
    11341 atccgttgag ccattttttc accccctgct ctattgatat cttttgggtt taacagaaga
    11401 tatttcagga gaataaccgg taaaaaatcg cactaactga aatcagtgcg attttaaagg
    11461 acacaattat acatgtttga ttgtatcata tttcattgct ttttcaagta taacacattt
    11521 ctatctcatt aagcatcaat ttgctactct tttcagtaac tgattttgaa tatcagcctt
    11581 ccagtacaat actcgaagaa caatatttac gagcatgaat acaaagaagt atcctacgct
    11641 ctctcccaac cctaagttta cttccaaaat tccatggaac agcattgcag ctacatatat
    11701 ccctacgatc gtcgttactg ctaggaagat gcgcaatggg ttgaagggat aacaagcttt
    11761 tacaactgcc atgcaactga tcccaatcag caagtaatac atcaaagtag tagtttcagc
    11821 atttccccat gcgaatgttt ttccaagtag atacaccgca atgatattca ctactacaag
    11881 caaggcattt ggtaaagcat taataagtgc tgtaggcaaa taacggtaag tgatttttct
    11941 tttatcttgt tcaaaagata aaaagaagga aggataccct tcaatcgcta aatcaatcag
    12001 cgtgatttgg atagggataa acggaaatcc aattgctgta acggcacata taatcgataa
    12061 aataaatgaa taaatcgttt tgataaaaaa gatgcctgag actttcgtca cattgttgac
    12121 cactcggcgt ccttcaaaca ggacttctgg caatgtagta aaatcagagt ccaacaatac
    12181 cagattcgca atctgacgtg ttgctccatc accttctgcc atagcaatgc tgcaatctgc
    12241 ttctcttaac gccaaaacat cattcacacc gtcgcctgtc attgcaaccg ttctgccgct
    12301 ttctttcaat tcatttacca gcaatttctt ttgctgcgga ctcactcgtc caaaaaccgt
    12361 atagcgatga gcagcttctc gcacttccat ttcttcagta atttgagaaa gatcgacata
    12421 cgcatcataa ttcggtaatc ctgcacgtct agcgatattg gagacagtaa aaggattgtc
    12481 tccagaaatc actttcagat ccacaccttc ctcatgaaga taagctagtg tttcttgagc
    12541 gttttttcgg attggatcat cgatttcaaa cagagcaacc ggctcaacaa atgctggttt
    12601 ttcttcgttc aattgctgat ttggagcgat ccccaacata agtacgcgat agccattcgc
    12661 ttgggctgtt tttagactgt ccggaagccg atcttcttcc accaaacgtt caggcgctcc
    12721 tacgtacacg actcccagtt ctggaaattc catcgcgccc catttgcgat cagaagaaaa
    12781 tgcgactttg tttgtcacat cataccgatt agaaagctca aaataatctc tcatagcccg
    12841 catagtgatg ttgttatcac tgctttctgc cagatacgtc cccataattt ctgggatggc
    12901 tttctcgtac agcggatgta gtgcttctac ccgctggata ctcattttcc cttcagtaat
    12961 ggtccctgtt ttatccaagc ataatgtatc gacatgggcc agcgtttcaa ttgaatacat
    13021 gtcttgaaca aggattcgtt tttttgccag tttggttacc cctgtcgtta atgcaatact
    13081 tattagcaat accaaaccct ttggaagcat gcctaacaaa gctgctgcag atgcaataac
    13141 agaaattttg atagaagcat cgcggatgaa cagtgcttcc aaaaatagta tgatccccaa
    13201 aggaataatc acgaaactag tgaatttgga tactttgcga atcgaatcta caagttcaga
    13261 acgaatagct ttgtgagtct ttgcctcgct agtcaagcgg acagcataat tatctgcacc
    13321 tactcggata acttcagcca tgacagaacc gcttgtcaaa aagcttccag aaagcaattc
    13381 agctccagtt tctttgacaa tcaaatcaga ttcgcctgtc aaaagagctt catttgcttc
    13441 tgcccgtcca gatataacct gtaaatcggc tggtatctgt tctcctgctc cgatttttac
    13501 caaatctcct aataccagct ctttagtaga aatcgattgt tctttaccgt cccggatcac
    13561 tagtacttct gattgggaaa caatcgataa tttccgtaca agatttcttg catgtatttc
    13621 ttgataaatt ccaataacga tattgacgac aataattgct aaatacgcca tgttggaata
    13681 cgcacctaca aataacagac aaatcccaat accaaagttc agaaaattga ataacgtcaa
    13741 tatattatcg ctgaatattt gttttgtact ttttgatgca tcttcctggt agtcattttg
    13801 ctggccagaa gccattttct gaactacctc ttgctgtgta agtccattca ttttcttttt
    13861 ctccattttc cgccaacttt caaaaataat cacgtgttta agtctagcgt gttccaagat
    13921 taaaaacaac ttgtttttat aaaatttata gaaaaacaca cgaatgattt tacaaaaaag
    13981 atgccaacct ttttgtttga atcacccgtg tgctgttttt attatcaatc tatctttact
    14041 tatattgttc ctccaaacgg ctcatccacc aacctagtgc tgtaccggct ataaaaagaa
    14101 caggacaagc tagtacccca gcaactccca atcccgcgta atcggtagga atgatcataa
    14161 tcgttgaagc aaaaactaca cctaaaatga aatggaatag cttgctatat gcctttgaga
    14221 aaatgtaatc catgattttt gacaatccaa gaacacaaag aatcccacca attccgatcg
    14281 gcacaatcac ttcaagtcgt acttctttaa atccgtcagc catcgcttta tacaatccca
    14341 tatacaccaa aaaattagaa gggctcaatc ctgggacgat catacccaaa ccaatcaatc
    14401 cgccggcaat cagccatgtt ccaaaattct gatcgacttg cccgtcaaat aattgagcgc
    14461 cgtataataa aaacaaataa gccagaacaa aagtaacaac catgatgatc acatcagaag
    14521 tatctcttcc ttttttccct gcttctttcc ataacgcagg aacagttcca gcgatacatc
    14581 cgacaaaaaa ccataatatc gttgtttcat acgcacctaa taaaaaactg actaaaaaag
    14641 aaagtagaaa gatgcctgtc actccgccta gaccaacagg cagaaaaaac aaaacatttt
    14701 ctttgaaatt ttttgtaagg tgggctaaaa aagaaatcag ccgctcatac atgccaaaga
    14761 cagctgctaa tgcaccaccg ctgactcctg gcaagataaa tcctgaaccg ataaacattc
    14821 ccttcacaaa ccgtaacatc cagtctttgg ttgtgtaccc cacttcttga ttttccattt
    14881 tttgcctttt ttccactatt tgcgtactcc ttctttcatt taattttatt catgattgaa
    14941 catttcctgc tattttactt aaagactttt tcataggtct ctattatttt ttcattcatt
    15001 ttgacaatat caggataaaa catgttacct ccattatgat agagatagcc gccattgtaa
    15061 agcaaggact ggatccgcca atatcggtat tgtttccctt cctcatttcc taaaagtggg
    15121 ctcagcacat ctttagaata cgcttctgct aattctactg tgttttctcc gccatgattt
    15181 tttacatagg caatataatc tttcccaaaa ttatatgctt ggacagctgt ccataaatcg
    15241 catccttgtt tctttgcttc tactatcatt tccgccaaat atttgacacc ttgatcaata
    15301 ctgtcagctg gattatcaat gatctcagct tctccatgca gactttccga gctctgcatg
    15361 agatctgtcc cttttccttt tgattcggtc aatattatcg ccttgatcaa attctcgtag
    15421 ttttccatat gataattttt tgcagctgtt gttatttcta tttgatagct ttccactcgt
    15481 tttaccgaac gataagttct tcctcataaa aacaacaccg caccaataac cgtcagaatg
    15541 atcatgtaaa aaagaatacg gataatagat aatcgttttc gcttcatttc atcacctagt
    15601 tattttgaca tcttttttta tcttactatt tctttatctt cttggctatt tcttccttga
    15661 tttctgtgta cgccttaaga aaaaatgaat tttcctgtat tttttgacat cttacgaaaa
    15721 tgatcgttgt ttttcagtaa aaacaattgc ccaatgactg atataatcgt tatactttac
    15781 acagtaggtg attatgtgaa aaaagaaaag aaatatgcaa aaatgccaga tgggagcgaa
    15841 atttactatg aaaagagcgg tcaaggcttc cctctctttt tactgcatgg caatgacggc
    15901 agcggccgct ttttttcaga acaggtccct gtactcgaac gctactatac cgtgtatcta
    15961 gtagatagcc gaggacatgg tcgttcgaca aacgaagcat ctatgttgaa ttttcagtta
    16021 atggccgagg atctgaatac gatcatgttg ctggaaaaaa tcgatcaggc agattttctt
    16081 ggatttagtg atggagcaaa tctagcactg gtatttgcca gcagcttccc taaaaaagtt
    16141 catcgattga ttttaaattc tggaaacacg cttgttaaag gcgttcgttt ttctgccaga
    16201 gtcatcagta atattcatta tgcatgggta tggttactct ctttatttcg cccaagtctt
    16261 cgcaaaaatc ttttagttat caaacttttg ctgcatgaca tcgggcttac agaaaatgat
    16321 ttaaaaaaaa tcaactcccc cactttgatc atcgtgggaa aaaaagatgt aatcaagcta
    16381 aaacattctt tgtatatagc taaaactata ccaaaagctt cctttgtgct tgtgaaagaa
    16441 caaggacatg aattagctag aaaagatcct gaacgcttca atcgagaagt tcttcaattt
    16501 ctttctgaaa cataactatt aggaatagaa ggtgatttat ttgttaaaaa ataccataca
    16561 atggctgaaa aaccacctag gactgttcaa aacaatcttt ttgatttctg tcattgtcat
    16621 tattgtcggt gaattgatgt ccatcggtaa aactctatct atccagcaat taggagaaac
    16681 gtttgccact atacctgtat ggaaaagcgg actaatgcta ttgattggtc ttatctctgt
    16741 gttacctatg gttggttatg atattatttt gaatcggatg cttgaacaaa agcagaatcc
    16801 tcgttacctg tttgagacaa gctggctgat caatacgatc aacaatattg ccggtttcgg
    16861 cgggtttgtt agtgtggggc ttcgctctga actatatgga caaaagaaag agagcagaaa
    16921 cgtgatccaa gcattatcga aagttttctt gtttttgatg gctggacttt ctatttacag
    16981 tctgctttct tttttacttg tagtcttcac gccagttgct ccttttttga agcaatattg
    17041 gatatggttg atcggcggcg gtctatattt tcctgtcgtc tttttattta ctactttcaa
    17101 aaagaaagga ttccttggtg gtacctcact taaaactcaa ggacagctat tgctagtttc
    17161 attgttggaa tggaccggtg tcctgtttag tttcctttct gttggttatt taatggaaat
    17221 ccggattgat ctgtggcaaa caattccatt attcattgct gcttcagtga taggaatcgt
    17281 gtctatgatt ccaggagaaa ttgggagttt cgatgtaatg atgattatcg ggttgtccgc
    17341 tattggtgtg ccaagagaaa tagtcgttgt gtggatcttg ctctatcgcc tgttctacta
    17401 catcattccg tttttgattg gaatcgtctt tttcttcaaa aatatcggtt ccacttttga
    17461 tcagcgttat tctggtatcc ctaaacaatt agcaacagaa attgcgcata aaatcgtggt
    17521 tgtcttatta tacttttctg gaatcatgct agtactttct gccactatcc cgcaagcttt
    17581 tacagagttt cgctggcttc atagtttgaa cccattgaaa tttcatttta ttatccaatt
    17641 tcctagtata ctattgggat ttcttttaat cattatgggc agagggatcg cagcacgtgt
    17701 gaaacgagca tatttgccta caatcttctt gattgttcta gctttacttt atgttttatt
    17761 aagtgatttt agtttcacac cggtggtttt cttgtctatt ctgcttctga tcattcttgc
    17821 ttctaaaaac gaattatttc gggaacaatt gatctattca tgggagtggc gtacgatcga
    17881 tggaatcatt attggtgcac tgtccttgct ttacatcgtt attggggtat acaatctgcc
    17941 ggatttccct catcggcgtc accattttat ttcctttttc ttatttcctt ctgaaaagat
    18001 ttggttttcg ggattgttgg ccatcatcgc tgttagtttc atgatcgttc tatttgttca
    18061 tttccttcaa ggagaaaaga aacagattgg cgaagccttt aatgaagaaa aggccctgaa
    18121 gatccttaca acttatggag gaaactccga tagtcaattg atcttcttaa aagataagcg
    18181 gatgtttgcc tatgaaaaag acggtgaacc aactgttctt ctccaattcg cttgcttcaa
    18241 taataaatgt attgtaatgg gtgatccttc tggtaagaaa gaagattttc cagaagcaat
    18301 cgaagctttt atcgaagaaa cagatcgact atgctattta ccagttttct acgaaacaag
    18361 cgaagagatt gttatgattt tgcatgaatt cggctacgat ttcattaaga tgggggaaga
    18421 agcttatgta gacctgaata gttttacaac ttcagggaaa aaaatgaagg gcactcgtgc
    18481 tgtcttgaat cggatcgaac gagaaggatt tacatttgat gtcttacaac caccattttc
    18541 tactgagcaa atgagtactt tcaaaaatat ctctgataat tggttgggtt cacgaaagga
    18601 aaaagggttt tctttaggct ttttctctga agattatcta caaagagcgc ctattgcagt
    18661 tgtcaaaaat acccaagaag aaattgtcgc ttttgcaacg atcatgccta cttacacgaa
    18721 caataaagta ggaacgattg atctgatgcg atatgattct aaaaaagcac cttctggcag
    18781 tatggatttc ttgttcttgc atttgtttgc ctatatgaaa gaagaacata tccaatggtt
    18841 caatttaggg atggcccctc tttcaaatgt cggaacttcc agaaagagtt ttttacagga
    18901 aaggattgca tacttggttt acgagtttgg ttctcatttt tattctttcc atggattaag
    18961 agaatataaa agcaagtacg ctacaaactg ggtctcacgt tatactctat attctagaga
    19021 tagttggatt gcatatgtta tgattgcttt gctgattatt gataatgcgc cagttgagca
    19081 gacacaaggt agaataagtg gtttgaaaaa atggatacga agaaagtatt aaaaactgca
    19141 taccgataga tgaacactct catgatgaat aaaagagact gaagtaaaac gaaagttcat
    19201 gcttctacaa tctcttctta tacaattgaa gaacaaagag cggtccaaac agaaatcctg
    19261 tttggaccgc tcttttattt taaacatttt tcattaccaa aatattcgag tcgcgtttct
    19321 tatgcttcga tctctgtaac gatacctgaa cctactgtcc gtccgccttc acgaattgag
    19381 aatgtcgttc cgttttcaac agcgattgga tggatcaagt caacatcaat cgtcacgttg
    19441 tcaccaggca tgaccatttc tgtatcttct ggcaatgtga ttgttcctgt aacatcagtt
    19501 gtgcggaagt agaactgtgg acgatagttg ttgaagaatg gagtatgacg tccaccttct
    19561 tcttttgtca acacgtacac ttctgctttg aattttgtat gtggagtaat ggaacctggt
    19621 ttagcaagta cttgtccacg ttcgatatcg tcacgttgga tcccccgtaa caacacgcct
    19681 acgttatccc cagcttcccc gtaatctaac gttttacgga acatttctac acctgttaca
    19741 actgcttttt gtgtttcagg tttgatccct acgatctcta cctcatcacc gacacgaaca
    19801 gcaccacgat caatacgacc tgaggcaaca gttcctcgac ctgtaattga gaagacgtct
    19861 tccactggca atagtaatgg tttatctgta tcacgttctg gtgttgggat atattcatct
    19921 actgtatcca tcaattccat aatagcagct tcagcatctg gatcgccttg caatgctttt
    19981 aatgcggaac ctttgatcac aggagtgtca tcgcctggga aaccatattc gctcaataac
    20041 tcgcgaactt ccatttctac caaatcgatc aattcttcat catcgactaa atcaactttg
    20101 ttcaagaaaa caatcaggta ttttacacca acttggcgtg acaataaaat gtgttcacgt
    20161 gtttgaggca tcggaccgtc agttgcagaa acaactaaga ttgcaccatc catttgtgct
    20221 gcccctgtaa tcatgttttt cacatagtcc gcgtgtcctg gtgcatcaat atgcgcgtag
    20281 tggcgttttt ctgtttcata ttctacgtgt gcggtattga tcgtaatccc acgttcacgt
    20341 tcttctggag ctgcatcaat actagcatag tcttgaggat ttgctaggcc ttttttgcct
    20401 aatactgtag tgatcgcagc agttaaagta gttttcccat ggtcaacgtg tccaatcgta
    20461 ccaatgttaa cgtgtggttt acttctgtca taatgttctt ttgccataat ttacaaacct
    20521 cctaaataat atagatcact gaaaaatttc agctctcacc tttttattat agtctttttt
    20581 tgactaaatc aaaaagaata cgtccgactt actttttgta agattcgaac taagcttatt
    20641 atacgtcaat attggtcaaa gtcaactaag agcactcgtc tgtcttatac gcgaaaaaag
    20701 taccttttat aaataaaaaa atgtgttttt tgagaaaaac ttcaagatag tccttaactt
    20761 tcttttgaag caataagaac aaaatgagga aaagtttaaa attcagctgt tgccaacaac
    20821 tttcaataga cgaaatcttc tatttcgtta ataataagga aagaggtgaa gtacatgaaa
    20881 aaattaattg gcgtacttgt cgttttaggc atatttctcg gcggaagttt tgcagcttat
    20941 cattattttt acggcggcga agcttattat acaaaaatca cgacgaatgg cgaaatgtca
    21001 gctacccaag cagataacgg agagaaattc acgacttata cctacaagca agcggcttat
    21061 aataaagatg gtgaaaaaaa acaggtgacg cttcgcgaag aaagagaaca tccattaaag
    21121 atgaatgcgt atcttaaact caaagtcaat cctcgtaaag gtgtattaag ctgggaagaa
    21181 ataaaggctt ctgaagtgcc taaaaatgcg gcagaaaaaa tcgataatta gtaaatggtc
    21241 ttaaaatttt attagataaa a
//
LOCUS       DAYYAO010000049        20184 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000049 DAYYAO010000000
VERSION     DAYYAO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.051"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="ACHARI_001714"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="ACHARI_001714"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8442762.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="ACHARI_001715"
     CDS             1013..2260
                     /locus_tag="ACHARI_001715"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8442763.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="ACHARI_001716"
     CDS             2336..2680
                     /locus_tag="ACHARI_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442764.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="ACHARI_001717"
     CDS             complement(2797..3123)
                     /locus_tag="ACHARI_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442765.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="ACHARI_001718"
     CDS             complement(3496..4698)
                     /locus_tag="ACHARI_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8442766.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="ACHARI_001719"
     CDS             4815..5330
                     /locus_tag="ACHARI_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8442767.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="ACHARI_001720"
     CDS             complement(5529..5975)
                     /locus_tag="ACHARI_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8442768.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="ACHARI_001721"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="ACHARI_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8442769.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="ACHARI_001722"
     CDS             complement(7854..8483)
                     /locus_tag="ACHARI_001722"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8442770.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="ACHARI_001723"
     CDS             complement(8467..8931)
                     /locus_tag="ACHARI_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8442771.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="ACHARI_001724"
     CDS             complement(9418..10686)
                     /locus_tag="ACHARI_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8442772.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="ACHARI_001725"
     CDS             complement(10917..11654)
                     /locus_tag="ACHARI_001725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8442773.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="ACHARI_001726"
     CDS             complement(11721..12290)
                     /locus_tag="ACHARI_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8442774.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="ACHARI_001727"
     CDS             12418..13290
                     /locus_tag="ACHARI_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8442775.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="ACHARI_001728"
     CDS             13632..14504
                     /locus_tag="ACHARI_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8442776.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /gene="nagE"
                     /locus_tag="ACHARI_001729"
     CDS             14501..16459
                     /gene="nagE"
                     /locus_tag="ACHARI_001729"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8442777.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="ACHARI_001730"
     CDS             complement(16710..17561)
                     /locus_tag="ACHARI_001730"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8442778.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="ACHARI_001731"
     CDS             17684..18151
                     /locus_tag="ACHARI_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8442779.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="ACHARI_001732"
     CDS             complement(18414..18896)
                     /locus_tag="ACHARI_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8442780.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="ACHARI_001733"
     CDS             complement(18996..19859)
                     /locus_tag="ACHARI_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8442781.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6068 a         4157 c         3663 g         6296 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tacaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc tttg
//
LOCUS       DAYYAO010000050        20169 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000050 DAYYAO010000000
VERSION     DAYYAO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.052"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1648)
                     /locus_tag="ACHARI_001734"
     CDS             complement(89..1648)
                     /locus_tag="ACHARI_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8442782.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1803..4037
                     /gene="pcrA"
                     /locus_tag="ACHARI_001735"
     CDS             1803..4037
                     /gene="pcrA"
                     /locus_tag="ACHARI_001735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8442783.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4054..6090
                     /gene="ligA"
                     /locus_tag="ACHARI_001736"
     CDS             4054..6090
                     /gene="ligA"
                     /locus_tag="ACHARI_001736"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8442784.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6196..6501
                     /gene="gatC"
                     /locus_tag="ACHARI_001737"
     CDS             6196..6501
                     /gene="gatC"
                     /locus_tag="ACHARI_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8442785.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6501..7973
                     /gene="gatA"
                     /locus_tag="ACHARI_001738"
     CDS             6501..7973
                     /gene="gatA"
                     /locus_tag="ACHARI_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8442786.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7970..9400
                     /gene="gatB"
                     /locus_tag="ACHARI_001739"
     CDS             7970..9400
                     /gene="gatB"
                     /locus_tag="ACHARI_001739"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8442787.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9410..10465
                     /locus_tag="ACHARI_001740"
     CDS             9410..10465
                     /locus_tag="ACHARI_001740"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8442788.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10576..11949
                     /gene="rlmD"
                     /locus_tag="ACHARI_001741"
     CDS             10576..11949
                     /gene="rlmD"
                     /locus_tag="ACHARI_001741"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8442789.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11952..12080
                     /locus_tag="ACHARI_001742"
     CDS             11952..12080
                     /locus_tag="ACHARI_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8442790.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12520..12708
                     /locus_tag="ACHARI_001743"
     CDS             12520..12708
                     /locus_tag="ACHARI_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8442791.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12786..13799
                     /locus_tag="ACHARI_001744"
     CDS             12786..13799
                     /locus_tag="ACHARI_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8442792.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13866..14900)
                     /locus_tag="ACHARI_001745"
     CDS             complement(13866..14900)
                     /locus_tag="ACHARI_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8442793.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15015..17240
                     /locus_tag="ACHARI_001746"
     CDS             15015..17240
                     /locus_tag="ACHARI_001746"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8442794.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17258..19141
                     /locus_tag="ACHARI_001747"
     CDS             17258..19141
                     /locus_tag="ACHARI_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8442795.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19122..20096
                     /locus_tag="ACHARI_001748"
     CDS             19122..20096
                     /locus_tag="ACHARI_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8442796.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6786 a         3707 c         4332 g         5344 t
ORIGIN      
        1 ttttcccagc ctcttctttt tttatagaat catatagaat caagtagtgt agatgaatag
       61 ttgttgtaaa atagtttttc catgttcgtt aaaatgattg cttcaaattt gtgatagaag
      121 atttagcctc gatttcctgc ttgattcttg gcaagtttgc ttcatcagtc aagataacca
      181 aaatatctcc tacattcatg atcgtatcac cgtgagtcaa gatttcctga tttcctctgc
      241 gtatagagat tagcagcata tttttaggcc aattgaagtc tcgtaccatt aatccgtcta
      301 aagaactctc tgccgttact ggaaaatcaa aagttgtcct tgcacctttt agtgaggcgg
      361 tctgagtatt tccaagtaga cgttccaata atgattcgta aattggatta ccgccgagaa
      421 ggtcattgac gatataagca acaagagcag ccaatcctaa aggcatcaga tgattaagag
      481 agccaaccat ttctgtcact aaaataattg ctgtcagcgg tgcttttcca atcgctgtga
      541 aataaccagc catggcaata acgacaaaat ccttgatgta gattggatcc atcccgaaat
      601 caaccataat gtttccataa aagctaccga ttaaagcccc caatgtcaat atcggaagga
      661 aaatcccccc tggaagaccc ccaccgtaag aaatcatgga aaaaacgaat cgaagaacaa
      721 acaaaccgat aatcataatg attggcaagg aagcatgtcc aaaagctaaa acgatttgat
      781 tgcctccgcc tagagtacta ggccataaat aaccaattgg caaaatcagc aagaaaggaa
      841 cgaacccgtg aaaataagct ggtaaaggca atcggttgta aaattttggc aaagccagca
      901 agacattctg ataaataaaa cctaagatgc ctagaagtac tcctaagatc actaaggaac
      961 cataatattt caacggcaaa gaaggaaggt cacctagata taaaacaggt ctcaagccga
     1021 aaaagtaaag agagacgaag ttagcagtga ttgctgaagc aaatgatgtc agccaaacaa
     1081 gtggagaaaa ggagtgatgg atttcttcta aaacaaagag taatcctgcg ataggagcat
     1141 tgaatgctgc tgcaagtccg ctacttgcac cgctagaaat taaaatcttt tcttctgaag
     1201 atggactccg aaacaattgt ccagctcctt gtccaatgga tgcgccaagc tgtatagaag
     1261 gtccttctct acctagaaaa aggcccgcac caactgaaag tacaccgcca atgaactttt
     1321 tccataaaac agagaaccag ttcattgata attccccgcg aagctgtccc tctacttgtg
     1381 gaatcccgct tcccttgata tccggttcat ttttgaccag ataacccacg aaaaatgctg
     1441 caaggatgca gcaaatagct aggccaatta agtaaatcgg ctgttcccga ctcatttcat
     1501 agatacttcg aactaaatcg cttagctttt cgatagataa tcgaaacagg ctaacgacga
     1561 ttcctgtcag cagtccaaca aatattcctt ttagaataaa atatatctga gtcttatcta
     1621 atttcttgat ttttcctttt ttttccattc aaacgcctcg ctttctttta atttagcgcg
     1681 aaatcaacga attgacaagg aaaaatccat aaaaagaaac aaatgatttt gaactagcta
     1741 gtttaatgaa agccaagaaa atgctataat taacaagatt gataaaagaa aggatgttgt
     1801 taatgtcatc caaagctgaa ttattaaacg ggatgaaccc ccgtcagaaa gaagctgttc
     1861 ttcacactga cggaccatta ctgctaatgg caggtgcagg aagtggaaag acgagagtgc
     1921 tgacacaccg cattgcctat ttgattgagg aaaaagaagt caatccttgg aacatcctag
     1981 cgattacttt taccaataag gctgcaaaag aaatgaaaga acgtgtcaat gcgattctcg
     2041 catctggagg cgaagatgtc tgggtatcga cttttcactc gatgtgtgtg cgtattttaa
     2101 gaagagatgt tgattttatc gggtataacc ggaactttac aatcatcgat tcttctgaac
     2161 agctgacctt gatgaaacgg atactaaaag aactgaacat tgatccgaaa aaatatgatc
     2221 ctcgaagtat attaggaaca atctctcaag caaaaaacag cctgcagacc cctcaggatt
     2281 ttgcaaaaat gcaaggcagt tattacgaag agattgcggc aaaatgttat gctgcttacc
     2341 aaaaagagct tcaatataat caatgtatgg actttgacga cctgatcatg aatacgattc
     2401 gattatttga agaacatcct gattcactca cgtattatca aaacaaattc cattatattc
     2461 atgtagatga gtaccaagat acaaatcacg cacagtatac gctagtcaat ttattggcag
     2521 gtagatttag gaacctgtgc gtcgtgggag atgcagatca aagtatttac ggctggcgcg
     2581 gtgcagatat gcagaacatc ttggattttg aaaaagatta tccagatgct gcagtaattt
     2641 tgctagaaca aaactatcgc tccacgaaaa acatccttag cgcagcaaac caagtcattg
     2701 aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga aaacaaagaa ggaaacaaga
     2761 tcacttacta ccgtgcagat aatgaacgag acgaaacccg ttttatcgta gaccggatgc
     2821 aagaagagat tcgaagcaat catcggaatt acggcgactt tgccatcttg taccggacga
     2881 acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc aaatattcct tataaaatgg
     2941 ttggagggca caagttctac gatcgtaaag aaatcaaaga tattttagcg tatttgaatg
     3001 tgctggtaaa tcctcaagat tcaatcagtt ttgagcgtat cgttaattcg cccaaacgag
     3061 gcatcggacc aggatctatc gaaaaactac gcagttttgc ttctcttcat gaatggccac
     3121 tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg tggaaaagca ggacagcaat
     3181 taggtgcatt tggagagatg atccaagaag tcactcagat gatcccatac ttgacagtga
     3241 cagaattgac gaaagaggtt ttggatcgta gcggatatct agaagactta aaaatccaaa
     3301 atacattaga agcacaagca cgcatcgaaa acttggagga atttttaacg gtcacacaag
     3361 aattcgacaa acaattcgaa cagcaaaatg aagaagacgc tgatgcacca gaagaaaaac
     3421 taacggtctt cttaaatgat ctggcacttg tttccgacat tgacaattta gaagaagatg
     3481 cttcccaagt gacattgatg actttgcatg cagccaaagg attagaattt ccagttgtat
     3541 tcctgattgg tctagaagag ggggtttttc ctttatctcg agcattgatg gaagaaagcg
     3601 aactggagga agaacgccgt ctcgcatatg tggggatcac gcgagcagaa gaagcgttgt
     3661 atttaacaaa tgctttctcc agaacattat atggacggac gcaatataac cgaccttctc
     3721 gttttgtaga agaaatcgat caggagttac tggaaatcga aggcatgcgt cctacgccta
     3781 aaaaaacgcc tgtattcgct aaaaaaacag catatagcta taaacagcca gagacagcgg
     3841 ttgttccctc taaaagtgct actggagggg aaaataacag ctggaaacct ggagataaag
     3901 tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt aagcgggacg tcgaaggatc
     3961 tagaactaga tgtggctttc ccaagtcaag gagtaaaacg tcttttggct gcctttgcgc
     4021 caattgaaaa agcttagtag tggagggtaa atgatggaac atgaaatgac gattgacgaa
     4081 gctgctaaac gagcagaaga acttcgtaca agactgaacc aatggtctcg cgaatactac
     4141 gtagaagata aaccaacagt ggaagactat gtttatgata aagaatacgc agaattagta
     4201 gcaatcgagg aacaatatcc tgatttgatc acgtctgatt ctcccactca acgagtaggc
     4261 ggaaaagtat tagaaggttt tgaaaaagtc actcatgata ttccgcttta cagtttgaac
     4321 gacgttttca gcaaagaaga gctgatagct tttgatcaac gtgtacaaaa agcagtggga
     4381 cgtgtagttg actattgctg tgaactaaaa atagatggac tttccgtttc tttgcgttat
     4441 gaagatggaa atttcgtgag aggtgcgact cgcggggatg gaactgtagg agaaaacatc
     4501 actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat taaaagaacc aatgaacatc
     4561 gaagtaagag gcgaatgttt catgccaaaa cggtcatttg tccaattgaa ccaagacaga
     4621 gaagcagagg gaaaagatat ctttgcgaat cctcggaacg ccgcagctgg ttctttgcgt
     4681 cagttagatt ctaaaatcac agcgaaaaga aatctggaca cttttttata cacagtagct
     4741 gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc tagaagagct agaaaaaatt
     4801 ggattccaca cgaatcggga aaaacgtcta tgtcattcga ttgatgaagt gtggtcatac
     4861 attgaagaat atcatgacaa acgtgtggat ttgccctatg aaatcgatgg aatcgtcatc
     4921 aaagtgaatg aattctcgct acaagatcaa cttggtttta ctatcaaagc accgagatgg
     4981 gcagcagctt acaagtttcc gccagaagaa gttgaaacgc taatcgaaaa catcgaatgg
     5041 acagtcggtc gaacaggggt agtaacgcca acagctatca tgacaccagt ccgagtagca
     5101 ggaacgacag taagccgtgc cagcctgcat aacggtgatt atattaagtt gaaggatatc
     5161 cgcttgaaag atacagttct gatttataaa gcgggagaca tcatcccaga agtttcgcaa
     5221 gtcgtgttag ataaacgacc taaagacagc gaagaatacc aattgcctac acattgtcca
     5281 gtttgtggaa gtgaacttgt ccatttggat gaagaagtag ccttacggtg catcaatcct
     5341 aaatgccctg ctcaaatgaa agaaggtttg aaccactttg tttcaagaaa tgcgatgaat
     5401 atcgatgggt tggggccgcg cgtactagaa cagatgtatg ataaaaaact agtagcagat
     5461 gttgctgatc tttacaaatt gacagaagaa gagttattga cgttggacaa aataaaagaa
     5521 aaatcagcaa ataatatttt gacagcaatc gacaacagca aagataattc ggtcgagcgg
     5581 ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag cagcaaaaat tttagcagaa
     5641 catttcggtg atctagaaac attgagtaaa agtgactatg agtcgattat tgcgcttgat
     5701 acgatcggtg atatcattgc cgacagtgta gtcacttatt tcagtaacga agaagtccat
     5761 gaattgatga atgaattaaa acaagcaggc gtcaatttcg aatacaaagg gcttcgaaat
     5821 gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa cagtagtcct tactggtaaa
     5881 ctaacacgat ttactcgtga agaagcaaaa gagacgatcg aaaatcttgg cgggaaagtg
     5941 actggtagcg tatcgaaaaa aacggatatc gtggtagcag gagaagatgc tggcagcaag
     6001 ctgaccaaag cacaagaatt aggaatcgaa gtttggacag aagatcaaat ggcagatgca
     6061 ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt ttttcagaca aatttcaata
     6121 aacaatttca gacacggcac tcttgtgtta aaatagagag caaagttttt aactcgttta
     6181 gaaagaaggg gtagtatggc gattagtgaa gaacaagtga aacacgttgc caaattagca
     6241 aaacttgcgt tcgcagatga agaacttgca agttttaccg accaattagg taaaatcatc
     6301 gatatggttg aaatgctgga agaagtagat acggaaggtg ttccatttac gtcgaatgtg
     6361 gttgaaacag taaacgtgat gcgacaagat cgggcgcaag ctggatggaa ccgcgatgaa
     6421 ttattgaaaa atgtgccgga aacagaagac ggctttatca aagtacccgc aatcatcgat
     6481 aatggggagg ctggcgcata atgacagaat tacacaatcg ttctctagaa gaactgcatc
     6541 atatgcttgt atcgaaagaa atcactgtac aagatttgac aaaaacaaca tttgaacgaa
     6601 tcaaagaaac agagcctgag gtcgaggcat tcattacatt gaacgaagaa aaagcattgg
     6661 aacaggcaaa agctttagat gaaaaaggga ttgatgaaag caacgtcctt gctggtattc
     6721 caattggtat caaggataat atcgtaacaa aagatatctt gacaaccgct gcatctaaga
     6781 tcctttacaa ttttgaacca atttatgatg caacggtcat ggataaaatc tatcaagcag
     6841 atatgatccc tgttggtaag ctaaacatgg atgaatttgc tatgggtgga agtacagaaa
     6901 cttcttattt caagaaaaca aaaaatgctt gggacccaac aaaagtacct ggtggctctt
     6961 ctggtggttc tgcagcagca gttgcagctg gacaaattcc agtttcttta ggtagtgata
     7021 ctgggggaag tatccgccaa ccagcagctt ttaacggcat cgtagggatg aaacctactt
     7081 atggccgtgt ctcacgtttt ggtttgattg cctttgcttc atctcttgac caaatcggac
     7141 caatgacccg tacggtgaaa gataatgcat tagcattgac agctatcagc ggttatgacg
     7201 aaaaagacgg tacttcttca ggcgtttcag ttcctgattt tgcagaggga ctgacaggcg
     7261 acatcaaagg aatgaagatt gctttaccta aagaatactt aggcgaaggt gtggcccctg
     7321 gcgtaaaaga agctgtattg aaagcagcag aaacattccg ttcattaggt gctgttgtgg
     7381 aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt ttattatatc attgcctcat
     7441 cagaagcaag ttccaatttg caacgattcg atggtatccg ctacggttat cgctctgaaa
     7501 atgtgaaatc attagatgat gtatatgtca actcacgttc agaaggattc ggtgaagaag
     7561 taaaacgccg tatcatgtta ggtacattct ccttaagcgc gggttattat gacgcttact
     7621 tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga ttttgaaaaa gtctttgcag
     7681 attatgactt gatcatcggg ccatcttcac caacagttgc attcggaatc ggtgagaaca
     7741 ttaacgatcc aatcacaatg tacatgagtg acatcttaac gatccctgtc aacttggctg
     7801 gacttccagg tatgtctgta ccggcaggtt tatcagaagg attaccagtt ggattgcaga
     7861 tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc ggcatatgcg tttgaacaag
     7921 caacagaatt ccataaacaa caaccagcta ttttaggagg gaaagacgaa tgaattttga
     7981 aacagtcatt ggactagaag tccacgtcga gttaaaaacc aactcaaaaa ttttctctcc
     8041 agcgccagct cattttggtg cagaaccaaa cagcaataca aacgtgatcg actggggtta
     8101 tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa ttcgggatga aagcagcttt
     8161 ggcattaaac tgtgaaatct ctaaagatac tcattttgac cggaaaaatt acttttaccc
     8221 agataatcca aaagcatatc aaatttccca atttgatcaa ccaatcggtc atgacggatg
     8281 gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg atcgaacgtg tccatttaga
     8341 agaagatgct ggaaaaaaca tgcatggtat tggcggttat tcttacgttg atttgaaccg
     8401 acaaggcacg cctttgatcg aaatcgtttc tgaagctgat atgcgttcac cagaagaagc
     8461 atacgcttat ctagaagcat tgcgatcaat tatccaattt acagaagtca gcgatgtgaa
     8521 aatggaagaa ggctctatgc gctgtgatgc caatatttct cttcgccctt atggacaaga
     8581 agagttcggt acgaaagcag aattgaaaaa cttgaactct ttatctttcg tgaaaaaagg
     8641 tttagctttt gaagaaaaac gtcaagcaaa agttcttctt tcaggaggag aaatccaaca
     8701 agaaacacgc cgttttgacg aaacaacgaa taaaacagtt ttgatgcgtg tcaaagaagg
     8761 gtctagtgat tatcgttact tcccagagcc tgatatcccg aaattcgtga tcgatgacga
     8821 atggattcaa aaagtaaaag ctagtttgcc tgagatgcca gcttctcgtc gcgaacgcta
     8881 tatccgtgag tttggacttc cagagtatga cgcaatggta ttgacactta caaaagaaat
     8941 gtctgatttt tttgaagctg ttttggaaaa tggtgcagat gccaagcaag catctaactg
     9001 gttgatgggt gaagtttctg cttacttgaa cagtgagaaa ctggaattag ctgagacaaa
     9061 attgacacct gagaacttag caggaatgat tcgtttgatc gccgatggaa cgatcagctc
     9121 aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat ggcggagaag cccaagaagt
     9181 agttgaaaaa aatggttggg tgcaattatc tgatccgaac aaactactgc caatcattaa
     9241 tgaaattttg gataacaacc aacaatctgt cgatgatttc aaaaatggaa aagatcgtgc
     9301 ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa ggacaagcaa atcctggtgt
     9361 cgtgaataag ctacttaaag atgaattagc gaaacgatag aggctgaaaa tgaaaaaagc
     9421 acgcgtaatt tataacccca cctcgggaaa agaattattg aaaaaaaatt tggcggatat
     9481 cctacaggca ttagaagaag caggatttga agccagcgcc tatgcaacta caccagaacc
     9541 agattcagcc aagaatgaag catgtagagt cgcagaacta ggttttgatc tagtagtggc
     9601 agctggtggc gatggcacaa tcaacgaagt ggttaatgga atcgctccgc ttgaacaccg
     9661 tcctaaaatg gccatcatcc cagctggtac aacgaacgac tatgctcgag cattaaaaat
     9721 tcctagagat aatattaaag cagcagcaga agtgataaaa aaacaccaaa ccgtaaaaat
     9781 ggacatcggc ctttctgata acagttactt catcaatatc gctgcaggtg gctatttgac
     9841 agaattgact tatgaagttc catcagaact aaagagtatt tttggttatc ttgcttatct
     9901 tgcgaaagga gcagagatgc tccctcgagt aaagccaatc aaaatgcgct tgaagtatga
     9961 tgaaggggaa tatatcggaa atgcttcgat gtttttccta gggctgacga actctgttgg
    10021 aggattcgaa cagatcgcgc cagatgccaa attggatgat gggaagtttt ctttaatcat
    10081 tgtgaagaca gcaaatatct ttgagatctt gcatattgca gcactcatgc tgaatggcgg
    10141 tcgacatgtc gatgatccaa gaataatcta taccaaaaca agcagtttgt tcgttgaaac
    10201 agaagatgat atcaatcgac cagtaatgat caacttagat ggtgaatacg gcggggaagc
    10261 accgatgcat ttccaaaatc ttcatcaaca cattgagttc tttgccaata ccgatcagat
    10321 tcctgatgaa gcaatcacag gtacagatga agaagaatta gaggttgtca gcaaagaatt
    10381 cgtaaaagaa gtcgaacgtt taacggatga agatatcgac ggtgacggaa aaatagcaga
    10441 cgaaacacct aatcaaaaga attaatcaaa agccggtgct gaacgtagta aggtcagtgc
    10501 cggttttagt tgtgtaatta gctgatttat agtagagttc agtaggattg aaaaataggc
    10561 ttaaaggagc aaaatatgac agaacagcaa atagtaaaga aaaacgaacg atatacagtc
    10621 gaaatcgtcg acttgagtta tgaaggaatg ggggtagcaa aaatcgatgg ttatccatta
    10681 tttatcgaaa atgcactccc tggagaagaa gtagaaatac tagtagtaaa agcaggcaat
    10741 aagtttggat atggaaaagt cgaaacattt attacacaat cacctgatcg tcagcctgta
    10801 gaaaacaatg cattacttcg cataggtatc gcacctttag cccatttgaa gtatgaacaa
    10861 cagcttgtct tcaaacaaaa acaagtagag catgtaatgg cgaagattgc taagatgcca
    10921 gaagttcctg tacttccaac ggtcggaatg gcagaaccat tcggctaccg taataaagca
    10981 cagatccctg ttcgccgtat cgatggacag ttagccaccg gtttttacaa aaagaatagc
    11041 catgatctag tccctatcga agatttttat atccaagatc ctgtcatcga tcaagcaatc
    11101 aaagtagtca gagatatctt gcaacggttc caagtacgag gatataatga agaaaaaaat
    11161 gaaggacagc ttcgtcacat cattatccgt cgtggtcatt attcccatga aatgatggtc
    11221 gttttagtta ctcgtaaaga aaaattcttt aaatccaagg aaattgctga agctattcat
    11281 gaagaattgc cagaagtggt ttcggtcatc caaaatatca atgaagaaaa aacaaatgtg
    11341 attttaggag acaaagaaaa agttttatat ggcagagact atatcgaaga tcagctatta
    11401 ggaaaaacct atcgaatctc agcaaagtca ttttatcaag taaatactca acaagcagag
    11461 tatctgtacc aaacagccat cgagtttgcc gagttagata aaaaagacat tgtgatcgac
    11521 gcttattcag gaataggtac gattggcttg tctttagcag acagagtgga taaggtgtat
    11581 gggatggaag ttattccaga agcaattgaa gatgcacagt tcaatgcttt aaataataaa
    11641 atcgaaaatg ttcattatga agtcggaaaa gcagaaaaag ttatgaaaaa atggcaagaa
    11701 aaagggatca aaccatctgt gatcgtagtt gaccctccac gaaaaggatt agatcatcat
    11761 tttattgaat cagcaatcga aatgtcacca gaacgaatcg tctatatctc atgtaatccg
    11821 gcaacttttg caagagatgc taagttattt gcagaagcag gttatgccgt gaagaaagtt
    11881 cagcctgtag atcttttccc gcaaacccat catatcgaat tagtcgcttc attcgttaaa
    11941 ggagaataaa aatggaccaa aaagaaaaaa tgaaagaatt gatcaaaaag aaacaaggtg
    12001 gaggccgatc caaacaaatg gaaacaccta agaatgatcg taaaaacatg agaaaaggcc
    12061 ctaaaatctt taataaatag acgtgctaaa gaaagactaa gaagtgttaa cttttgttat
    12121 tgtttcttag tctttttagt tagtccacat tattttggcg atttgccaat aaggactgtt
    12181 gagttgtgca aaatcaaatc tgggtcagaa tgaacgtcat tctgtctcag atttttcgtt
    12241 atttcaattt gattaactga ttgatcaaaa agatttttat tttcatattc tcaaatgatt
    12301 caacactaaa tatacatgtt tgacgcggaa attattattc gattttggcg gcatcagggg
    12361 ggatgacgac gtagcagacc cattatttta ctcaagaaaa tgtccgtgcc tattatttag
    12421 aatacgatag tgaacgtgct gggagttttg ttcttttaga gaaagtctct gaagataaat
    12481 tagttgtctt agattttgac tgtaacgaaa aatggcgaaa tggaagagca ccagcaagtg
    12541 attgaccgga tctacgaagc gacacgatat ctgcctttgg atcgtttgtg cttaagtcct
    12601 caatgtggat ttgcttcaac agaagaagaa aatgttttgt ttgaagaaca atggaaaaaa
    12661 tttcttttgt caaaagcatt gcagaagaag tttggggaaa atcattaaat catctaggag
    12721 agcttgatag gaaataacac tatggtataa tcagaatgct taatactaac tactgaggga
    12781 gccttatgaa aaagaaaatt cttaaattta tcggatggtt cttcggaatc attcttggga
    12841 tcgcgtttgc aatctttctt gccttccagc tttcccccag acctggagca ttcttgatca
    12901 accatatgtt ttcaaatact gttcaaatca ctgataaaaa aacgttcgat caagcagaaa
    12961 aaaacgtggc gaaaaaaaca gatttgacgt atgagtccaa gtataaagag aatacttatg
    13021 acatctacta tccaaaaaac agcaaggggc ctgttcctgt tctattttgg gtacatggtg
    13081 gaggattcgt cggcggagat aaatcgggtg tcaaagaatt tgcgacaaaa ttagtttcag
    13141 acgccaacat cgcagtggtt gcgatgaatt atgaattagc gcctgattca gaatacccta
    13201 atcaagttct tcaagtgaat gaactgatca aagatttatt agctgaagaa aaagacgaca
    13261 aattgctaga catggaacaa ttattttttg gtggagatag tgcaggttca caaatcgcat
    13321 tgcaatatgc agctatacaa acgaatgatg cgtatgctaa acaaatgaaa atcaaacagc
    13381 tacttccaaa agatagtctg aaaggaacca tttcttattg cggacctgtt gatttgaagc
    13441 taacagcaca gcagcattcg gatgatcgat ttatgaagtt tttcgtcaaa accgttgcgt
    13501 ggtcgttgat tggaacaaaa gattggaaaa cgagtgagca attgaaagaa gctagtgtgg
    13561 ctgaccatgt ttctaaggaa tttccgccaa cgtatataac tgacggaaat gcatactcat
    13621 tccaggagca aggtcaagca ttagaaaatc ggttgacaga attaaaggta cccgtgcaat
    13681 ccttgtttta tacagataca aaaaaagaga ttacccatga ataccaattt gattacacgc
    13741 taaaagaatc gcaaaattgt taccagcaga cattggattt tgtgaataag tataagtaag
    13801 aggttgtgaa aaagccgttt cgttttgagt atgaagaaac gaagcggctt ttcacaacct
    13861 ctctttcaat acctttttgt tcttgggata aaactttccc taacaatcaa tcgtgcagga
    13921 taagtcaaaa tgattggttc gtcccgtata ccgtcaattc gctccaatgc cattcgaaca
    13981 gctgcttgcc caattgattt cgcattgatt tgaacactgg tcaagccagg ggagaggttt
    14041 gcgattgtct cgatattatc aaagcttgct acaacaatct cttctccaat ttggatattt
    14101 ttttgtttca atgcccgata gacaccaatt gccattggat cgctcccgac aagaatggca
    14161 gtaggaagag gggattcatt ttctaataga ttagtcgcaa gtacctcacc ttgtttttct
    14221 gtccagccac ctagatgata agacatatat ttttctaagt gatgatcgat cataaaatgc
    14281 tgataggcac gcatcctctt ttcatttata gaaatctgct tcccaccgaa ttcatccata
    14341 tcgactttat atcccccgat ataggctatt cttttatgac cattttctaa gaacaattcc
    14401 agtacggttt tagtcatccg ttcaagatct gcatacacca tatcgacctc ttgctggatg
    14461 tcagggttat ccacgacgac tatttgcatg ttttgtttta acaaggcggt aatcgattct
    14521 ttcgtaacag tcccaacaac gatcaccgca cctaactgat ctagatcttc ccattgttta
    14581 ggattatcag atagattata gattcgattc atccctaaat gcagacgtcg agcttcttct
    14641 tccatcccta ctcgcaaatg tttgtaatag atatcctgtt cttcgctcgc tttgccgatc
    14701 gtagagatca caccaatcga aggcattctc tttttgatat atttacgagt actgatgtca
    14761 taatccaacg cagcagctgc ctctactatc cgttgccgag tagtctgagc cacatggatc
    14821 gtctgatcgt tattcaacac acgggaaact gtagcaggag agacatttgc ctttcgtgcg
    14881 acatctctaa ttccaaccat ttttacacac tcctcaaaaa aataaaaaat ttttattgac
    14941 aaaattgttt agtaaacagt atacaggaat tataaaaaaa agtaaacggt ttccgttttg
    15001 ttgggagtga aatgatggat attacgcata gaaaaacaga taaaaatttc atcttatttg
    15061 atgaaaagag ccgagtcttc catttacgaa atacatttct ttcttatctg ataaagatag
    15121 aagaaagcaa tgtattggcg catatttatt ttggcaaacc agtcaagcag tacaaagaca
    15181 ataaaaatta tccccgaaga gatcgtggat tttcaggaaa tgttccctta aatcctgacc
    15241 ggtctctgtc gaaagataca ctaccgcaag aatactcttc tcatggcagc atggactttc
    15301 gaacgcccgc atcgatcatt cagcgcaaga atggatcaga cttactggat ctgagatatg
    15361 acagccatta tattacagat gggaagccag agatcgaagg cttgcctcaa acttatgtga
    15421 tggacaagtc agaggcacaa acgttggtga tttcacttaa ggatagagaa acagcgattt
    15481 atttcgattt gttctatacc atatttactg atagagcagt gatcacacga tctgtaaaga
    15541 taagaaatga aaccggagaa acaatcaaac tagaaaaagc ggctagtttc cagctagact
    15601 ttgcccatac taggcgattt gatgaagtca ttgctttgcc aggagcacat gtcaatgaaa
    15661 gacagatttc acggcaatct gttttatctg gaacaaaagt atttgaaagc cgtcgaggaa
    15721 cgagcagtca ccatatgaat aactttattg ctttagttca tcatcatacg acagaaaata
    15781 ctggagaagc gatcggactt caatttgttt attccggcaa ccattctttc gaacttgaaa
    15841 aagatcagat caatcaattg agagtcgtcg gtgggatcaa cagccatcgt ttttcgtggg
    15901 aattgaatgc aggccagtct tttcaaacac ctgaaatgat cctttcttac tcaagtcagg
    15961 gattgaacaa aatgagtcag atccaccatg agttgctacg tgaaagaatc gcaagaggtc
    16021 gtcatcaatt tgctgaacgg ccgattctgg tcaataactg ggaagccact tactttgact
    16081 tcaatagtga aaaaatcaag gcgatcattg atgaagcaaa ggagctaggc atcgaaatgt
    16141 tcgtattaga tgatggctgg tttggaaaaa gagatgcgga caattcttca ttaggagact
    16201 ggttcgagta tgaaggcaag ttgacaaatg gattacgtga aatagcagat tatgctcata
    16261 gcaaaggatt gaaatttggt ttatggtttg aacctgaaat gatctcggtt gattctgaac
    16321 tgtatcgaac acatcctgat tttcttatgc aggagccagg tagaatgcct tctgcttcca
    16381 gatcacaaca tgtactggat tttacaaggt tagatgttcg gcagacgatt gagaaacaaa
    16441 tgagaaagat tttagatacg attcctcttg actacatcaa atgggatatg aaccgttcgc
    16501 tttcagatgt ctattcgatt acccttgacc cacagagaca aggagaagta gcgcatcgtt
    16561 atatgctcgg gctttatgaa ctacttgaac atttgacaac tgattatccg gaaatcttat
    16621 gggaaggctg ttctggtgga ggtggacggt ttgatgccgg gtttatttat tatatgccgc
    16681 agtcatggac aagtgataac acggatgcag tggagcgtat gaaaatccaa tatggaacaa
    16741 gccttgctta tccgatttcg tccatcacag ctcatgtatc agctgtacca aatcatcaga
    16801 ccgacagatc tacttcgttg aaaacaagag gagaaacagc tatgagcgct gtttttggtt
    16861 atgaactgga tttgacaaag ctgtctcctg aagaaaaaaa gcaagtaaaa gaccaaatta
    16921 tatcctatca aacgattcgt ccggtgatcc agtatggcca ttattatcgt ttggcgagtc
    16981 cttttgaaga aaatattgcc gcatggatgt ttgtgtctcc taaacaagat gaagctattg
    17041 tgtttttagg gaggatacta gcttcagctc aaccagcgtt ccatgaagta tatctgatgg
    17101 ggttagatga tgaggcactt tatcaggaac agacctcgaa gcggatattt tcgggggccg
    17161 aattgatgac agttggactt tacttccccg attttcaagg tgatttccaa acagaactgc
    17221 ttcatttcaa aaagttatga gagagaagga aaaaagtatg aaagcaaata taaaatggtt
    17281 agatgatcca caagttttta gagtgaacca atcaaatgca catagcgatc atgtttgtta
    17341 tgcaacaagg gaagaagtag aaaccacatc ctcccttaaa cagtctttaa atggatcatg
    17401 gcgtttttgt tattcgccaa atccttcggt acgaccaaaa acattttata aaaaagattt
    17461 tgatgcctgt tcttttgatg aaatcactgt tcctagtcat attgaattag caaattatga
    17521 caagatccat tacatcaata cgatgtatcc gtgggaggga catgtatttc gccgtccccc
    17581 tttcacgaca agccctgaga aagaggcgga agggatcttt tcagctgcta aagacaatcc
    17641 ggttggttcg tatttgacga cttttcgatt agaagaaggt ctaaagggaa aacaaatctc
    17701 gattcatttt gctggtgtag aacaagcaat gtttatttgg ttgaatggtc attttatcgg
    17761 gtatgccgaa gattcgttca ctccgtctga atttgattta acaccttatt tggaagaaga
    17821 aaatattcta gctgtggagg tttacaaaag aagtacagct gcattcttgg aagatcagga
    17881 ctttttccga ttttttggga tcttcagaga tgtcttctta gttgctcgag gacaaaacca
    17941 tttagaagat ctttggatca aacctcgctt agatgacaaa tgtcaaaatg gcaaacttga
    18001 agtagatttg tcttttgagc aagtagccaa aaacagtcag ctgaatgtag cgatactaga
    18061 tcctgatggg aaggaaatct atcaacagca atatccgtta aaagagcaaa tgacgattcg
    18121 catcgatgga tttgaaaatg tggcactgtg ggggcatttt cagcccaatt tatataagct
    18181 tgaattggag acgatagacg aagaaaaaag agtaacggag tatattgact attccttcgg
    18241 ttttcgtaaa atcgaaatca aagataaaat catctacttc aatggaaaac ggatgattat
    18301 caacggggta aatcgacatg agtggcatcc gaaaaaagga cgcgccatca ctatagaaga
    18361 tatggaaaaa gatttggcta tcataaaaaa tgcaaagatc aacggcgtac ggacatctca
    18421 ttaccccaac cagatcactt ggtattattt atgcgaccaa gcaggaatcc atgtgatggc
    18481 ggaaacgaac ctcgaatccc atggctcatg gcaaaaaatg ggcgcatttg agccttctta
    18541 caacgtacca ggtgcagtac ctcagtggca ggcggctgtt cttgatcgag caaaaacgaa
    18601 tttcgaaaca ttaaaaaacc atccaagtat tcttttttgg tctcttggaa atgaatctta
    18661 tgcggaagaa aacattcgac aaatgaacga ttacttcaaa aaagtagacc ccgatcgact
    18721 tgtccattat gaagggaatt ttccaaatcc agcattcgaa gattcgattt cagatgtaaa
    18781 aagctgcatg tatgcgccac cagaagagat cattgactac cttgaaaata aagcggaaaa
    18841 accgttcatc ttatgtgaat atatgcatga catggggaat tcgttaggtg gaatggattc
    18901 ttacatgacg ttgcttgatc gttatgaaat gtaccaagga ggctatatat gggattttaa
    18961 agatcaggcg atccaagtga ctgatccaat cacaggcaga gaagtgatgc gctatggcgg
    19021 tgactttgac gataaaccaa gtgactatga attctcaggg aatgggatcg tttttgcaga
    19081 tggacaagaa aaacccgcca tgcaggaggt aagatattat tatgaaaaat acagtaaata
    19141 aaagtcatat ggatacggaa aaagttgcaa tcgtcttcgg cgactgtaca ttaggtgtca
    19201 aatcggggaa tacgcattat attttttctt atacaagagg cggactggaa tcgctcaata
    19261 aaaacggaaa agaatggcta taccgggaaa cacttccagt tttttggcga gcaacgaccg
    19321 ataatgacag aggtagcgga tttggattta aatccggtat gtggttagga gccggattgt
    19381 atccaaaagt caaagaaata gaagtactgg tggacaataa aaagatcgat ttgccgattg
    19441 ctccagtaaa taatcaatat tcaagtaatg aatacgctga aacggttgaa atcattttta
    19501 cactggaaat caacacagta ccaaaaaccc aagtgacaat ttcctaccaa atcgctggag
    19561 agggtaaaat gcttgtcaat atgcattatt atggacaaaa aggattaccg gaattacccg
    19621 cattgggttt aaggttcatt atgccaacga aagcaaaatt ttttgagtat gaaggcctat
    19681 caggggaaac atatccggat cggatggctg gtggtgtcga aggtatttat aaagtggcag
    19741 gactccccgt ttccccatac cttgttcccc aagaatgcgg catgcatatg gaaacgaaat
    19801 gggtagaact aacaagaaat caaacgttgg ataatacgga taaagatcaa gaagattttt
    19861 cgttgcgatt tgaacagcag gatacgccat ttgctttttc cgctttacca ttcaaagcag
    19921 aagaactgga acaggcaaca catatagaag aactaccgcc agaaagacga acggttctta
    19981 cgatttttgc aaaagtaaga ggagtcgggg gtatcaatag ctggggagcg gatgttgagt
    20041 caccttaccg aatcagcagc gaagaggacc atgaaatcgg ctttattatt cagtagcaat
    20101 ctttagatta ttatttgaaa aacggagttt acaagacaaa gctgctttat tctatatgtc
    20161 atgaacaag
//
LOCUS       DAYYAO010000051        19664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000051 DAYYAO010000000
VERSION     DAYYAO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.053"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..1011
                     /locus_tag="ACHARI_001749"
     CDS             115..1011
                     /locus_tag="ACHARI_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8442797.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1161..2102)
                     /locus_tag="ACHARI_001750"
     CDS             complement(1161..2102)
                     /locus_tag="ACHARI_001750"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8442798.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2228..3349
                     /locus_tag="ACHARI_001751"
     CDS             2228..3349
                     /locus_tag="ACHARI_001751"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8442799.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3607..5109
                     /gene="eat(A)"
                     /locus_tag="ACHARI_001752"
     CDS             3607..5109
                     /gene="eat(A)"
                     /locus_tag="ACHARI_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8442800.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5176..5703
                     /locus_tag="ACHARI_001753"
     CDS             5176..5703
                     /locus_tag="ACHARI_001753"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8442801.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5914..6975
                     /locus_tag="ACHARI_001754"
     CDS             5914..6975
                     /locus_tag="ACHARI_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8442802.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6978..7874
                     /gene="murQ"
                     /locus_tag="ACHARI_001755"
     CDS             6978..7874
                     /gene="murQ"
                     /locus_tag="ACHARI_001755"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8442803.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7888..9870
                     /locus_tag="ACHARI_001756"
     CDS             7888..9870
                     /locus_tag="ACHARI_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8442804.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9935..10381)
                     /gene="nrdI"
                     /locus_tag="ACHARI_001757"
     CDS             complement(9935..10381)
                     /gene="nrdI"
                     /locus_tag="ACHARI_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8442805.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10592..12016
                     /locus_tag="ACHARI_001758"
     CDS             10592..12016
                     /locus_tag="ACHARI_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8442806.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12197..12970)
                     /locus_tag="ACHARI_001759"
     CDS             complement(12197..12970)
                     /locus_tag="ACHARI_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8442807.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12967..13872)
                     /locus_tag="ACHARI_001760"
     CDS             complement(12967..13872)
                     /locus_tag="ACHARI_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442808.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14026..14346)
                     /locus_tag="ACHARI_001761"
     CDS             complement(14026..14346)
                     /locus_tag="ACHARI_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8442809.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14820..15506
                     /locus_tag="ACHARI_001762"
     CDS             14820..15506
                     /locus_tag="ACHARI_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8442810.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15508..16452
                     /locus_tag="ACHARI_001763"
     CDS             15508..16452
                     /locus_tag="ACHARI_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8442811.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16651..17295
                     /gene="pcp"
                     /locus_tag="ACHARI_001764"
     CDS             16651..17295
                     /gene="pcp"
                     /locus_tag="ACHARI_001764"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8442812.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17374..18651
                     /locus_tag="ACHARI_001765"
     CDS             17374..18651
                     /locus_tag="ACHARI_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442813.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            18909..>19664
                     /locus_tag="ACHARI_001766"
     CDS             18909..>19664
                     /locus_tag="ACHARI_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8442814.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         6318 a         3508 c         4160 g         5678 t
ORIGIN      
        1 tattttcata caaatgttaa attttcatac aaatatagga aacgttttac tggcttttta
       61 ctatttaaac aggctatcat aggaaaaagt gacagagaaa gtgaggcgga aaagatgaac
      121 aaaaatgacg tactgctagt tgcaaaaaat aaatcacttt attttttgcc ttccatccgc
      181 tctttagatg tttggtccgg aaaagattta ctacagtcgg aattcattaa atcagctaga
      241 caacagttgg caaattatca gctgatcgtt tttttagatt atggatttga tatggagatg
      301 gctcgacgcg tgcgcccatt taccaatgca aaaattgtgt tatttttttg gaatcacttc
      361 aaagcagaac acgaggcgat gctgaagcag gcacaagatg aaccggcaat cgatgagatt
      421 tatcattttg attttttgga agcaaaagaa cttgggctga aacataatag ttcattttat
      481 tcgaaacata tgaatttttc ttctagcgaa ccaaagacgg atctattttt tggtgcaacg
      541 aataatggga gaaaagaacg agcagaaagc tataagcagg aatttataaa aagagatgtt
      601 acgaccaact atttcattct tccacatcga gggaatgaac aacctggata tcttacttat
      661 gaagagtacc tgaaaaaaac aggggaatcg cgcgggattc tggaactgtt gcgggagggt
      721 cagcaaggag tgactttgcg gacatttgaa tccatgtatt ttcaaaagaa actagtgacg
      781 gataatcaag cgattgctca ttatcatttt tatcgtcctg aaaatatttt tttgcttcga
      841 gaacgtgatt tagatgaatt accggaattt ctgcatacac catttaagcc aatcctatca
      901 gagctattgg aattttatga tgcagaaaat tgggcaaaac gatttatcac taacgattcc
      961 actatttatg agcaatacga atatgacctc tcctctgttg aaaacaaata aaaaacctac
     1021 attttgaaga gattatgtac tgaaacaaac aagcacaatt cttatggcat catgagagtt
     1081 gtgttttctt tttgatcttt tattgggctt aaaaaaagag aaccagctgc tgattcagct
     1141 gattctcatc agagagcctt ttattctgcg ccaaggattc cttcttttgc atggactaat
     1201 tgggtcaaaa aggcacaata tggtgtagca atatcatatt tttgtccttt tcgccaaatg
     1261 gcaccattga tgtagtcgat ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata
     1321 gatgggtaat gcaacccgat gttgtcagga tcataagtag aagcaatatg ttggtacact
     1381 tcttcttgat ctagtatgat tccttctttt gctgcgatag aagcaaattc agatacgatt
     1441 gttcgaacca tgctctctgc cgctttttgc gcgcctagtt cagcaatatt acaatccaaa
     1501 atcgtgcata aaccattcaa tgttccattt acacaagctt ttcgccaaat ggaataacga
     1561 acgttatggc tatagatcgg atttaaattt gcttcttgaa agacttctac aacttttttt
     1621 gtaaatgctt cgccttctgg ttccaagttc tctagttcga tttctccatc acctaacaat
     1681 gtgacttttc cgggaccagc tagaccagct gtccacatcg tgatcccaaa taagatattt
     1741 ttctttggca catatttttc cagtacatct tcatgaccta atccatttag taaacacaaa
     1801 acataagtat tgtcagtaat aatggattgg atcgaacaaa acatatcatc taattgattg
     1861 gcttttgtta atgctacgat caagtccacg tgttcgttgc tttcgatgat ttcttctggt
     1921 gaatagattg gcagttttgc gaccacttct ttaccgttaa agtcagcaat caacccattt
     1981 gtacgaatgg cttcaatgtg agcaggccaa cgatcgatca agaggacctc atttccgctt
     2041 tgatgtagca tcaatccgat tcggctgccc attgctccgg ccccagctat tgcgattttc
     2101 atgttttttc tctcctttat tcaatgagtt cccttttagt atatagaaaa atggaagaaa
     2161 atagcattgt tttgtttgaa aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg
     2221 gatacagatg gatattttgg cggcaattga ttcatttaaa ggaagtgctt cttcatttga
     2281 actaaatcag gcagttctaa atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc
     2341 gattgcagat ggcggagaaa atactgcaga agcaatctat tacgcgctca ggggaacatg
     2401 gcgtaccatt gatgggatgg atctcttatt ccgtcagcga aaaatcagct atttattgac
     2461 aacttggcaa ggaaaaaaca ccgcagtcat cgaatctgca caagcattgg ggttagatct
     2521 acttgataaa ccaacggatc aaactatccg acttgcttcc agttatgcct taggagaact
     2581 tattaaagat gcactgcttc aagaagtaga acaaattatt gtcactttag gaggaagcgg
     2641 gtgtagcgat ggtggtctag gattactcca atccttgggt gcttctttag aagggtacac
     2701 agaaggaaat cctttattat ctactaaaaa acttcatttt gatcgaagaa acgttcttgt
     2761 ttctaaagta aaattgatta tcgctgcaga tgttaccaat ccatatacag gtcaacaagg
     2821 agctgcttgg caatttggaa gacaaaaagg aggaatagat acgacgctag ctttcttaga
     2881 taaacaagca ggaaagattg ctcagcaaat aaaagatttc tgggaggtgg atttagatga
     2941 tattccagga agtggagcag caggcggtat tggtggagcc ctttttttgt tgggagcgaa
     3001 gatggtttct ggtttcgatt tagtcagcca aatcactgga ttggaaaaaa agatcgaaca
     3061 agcagatgtc atcattacag gagaagggcg gatcgatcag caaacgatcc acggaaaagt
     3121 tccatacggt gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg
     3181 aagtagagac caagagctag aaggactttc tgattttctc ccaattgtct taagtattca
     3241 tttgggtcca ataggtttag aagaagcaat ggaacacagg caaacattag aaaaaacaaa
     3301 aatcttagga cagacactta gcagattttt caccatcctc ccgaagtagt tgacaaaatc
     3361 attttcttta tgctatcgta atttcaaata taggacaaag tgagggatcc gtgcattgaa
     3421 aaattaagtc gattgatgaa agctgcattc agactgtacc ttgtggagac aaggaagcag
     3481 tgtgcagatt ttcgggattg gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc
     3541 aacccttgca tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga
     3601 tggaaaatgt ctaaaatcga aataaaaaat ctgacattcg gctacgacag ccaaggcaca
     3661 ttattatttg aacaagcaaa tctaaatttt gacacacaat ggaaactagg acttatcgga
     3721 cgaaacggtc gaggaaagac aactttactg aatattctac aaaacaaact accttatcaa
     3781 gggcaagtaa tccatcagca agaatttgcc tatttcccgc aacagacaaa agataaagaa
     3841 cgtttaacct attacgtgtt aaatgatatt acggattttg agatatggga aatcgaaaga
     3901 gagctccaat tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatcg
     3961 gggggagaga agacaaaagt cctactggca cttttatttg tggatgacac tcatttcccg
     4021 ttaatcgatg aaccaacgaa tcatttggat atctctggta gaaaacaagt agcggcttat
     4081 ttgaaaaaga aaaaacaagg cttcatcgtg gtcagccatg accggggatt tatcgatgaa
     4141 gtagtggacc atgttttagc aatcgaaaaa agtcaactgg aactttatca agggaatttc
     4201 tctatctatg aagaacagaa aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa
     4261 ttgaaaaaag aagtcagtag gctaaagaaa acagcagctg aaaaagccga atggtctcgt
     4321 tcccgagaag gagataaaac aaagaaacaa gtcggattca tcgatactga atctagacga
     4381 gtgaataaag gagcagtggg tgctgatgct gcacggacga tgaaacgatc caaagcaatc
     4441 gtgaatcgga tggagaccca gatcagcgag aaagaaaaac tattaaaaga tatcgaatat
     4501 atcgattctt tgacgatgaa tagccaagcg tctcaccata agcgactttt aagcgtagaa
     4561 gatcttcaat tagggtatga aaatctgtta ttcgagccaa ttcattttac aatcgagcct
     4621 catcagcggg tggcgatttc aggtcctaac ggtgcaggaa agtcatccat tatccattat
     4681 cttctggggg cattcaacgg caaggttata ggagaaaaat accagccaaa acatctgagc
     4741 attagttatg caagccaaaa ttatgaagac aatcgaggaa cgttggcgga atttgcagag
     4801 aaaaaccaag tagactacca agcatttttg aacaacctcc gaaagcttgg gatggaaaga
     4861 gatgtttttc ataacaagat cgagcagatg agtatgggcc aacggaaaaa agtggaattg
     4921 gctaaatctt tatcacagcc agctgaacta tatacatggg atgaaccatt gaattatttg
     4981 gatgtcttca atcaagaaca attagaacaa ctgatcttga acgtgaaacc tgccatgtta
     5041 ctagtggaac atgatcaaac cttcctggat aaagtatcta ctgagattat ttctcttgag
     5101 agaatctaac ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac
     5161 gaaggagaga atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa
     5221 ggattagttt gcggacgggc agatgtgccg ctgactgaaa aaggacagat gcaggcacag
     5281 aaactagcag aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct
     5341 cttcaacgag caagagatac agcacaagca gttgctgatc gtctcagctt gccgctgacc
     5401 gttgatgaac gattggtaga aatggatttt ggggattatg atggactgcc aagcaaagat
     5461 gagaactttc aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc
     5521 ttagatgtgt atgcacggat cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat
     5581 gtctatctgt tagtttgcca taatgccttg ataagagtga tcaatgctta ttttcatccg
     5641 atgcctaatg aggggttctt tacattcatg gtagataata cagaattggt ttcttatgag
     5701 taattatgtt tttgtgttat ttaaataaaa tatttcaagg cactgctttt gcttagcagt
     5761 gcctttttta ggttatgaca attcttttga taatataaac tcaaccttta gaaatgaaat
     5821 ttaattattt taataaaagt attgaaattt tgtttcttta gatatatact ctagtcatag
     5881 aaatgaagac ggatacagaa aaagagggat aatatgtttg gcttttcaat atttatgaac
     5941 gattctttgt cagaagagaa gaaacagtat atccgccaaa tggcatccaa tggctttgtc
     6001 ggtattttca cttccatgca tataccagaa gatgatgttt cggcatatca aaagcgattg
     6061 atcgaactag gaagcgaagc gaaaaagaat aaactagaat tgatggtcga tatttcagga
     6121 gaggcattgg aacgagcggg tttttcatta gaagatctaa cgcctttgaa agcaattggt
     6181 gtaacaggtt taagaatgga ttatcatatt tccaatcagc agattgccga ctggtcacat
     6241 caattgaaaa tcagtttgaa tgctagcacg atcacgccta aagatatcga tgaattgaaa
     6301 gaagcagaag ctgatttctc acagattgaa gcttggcata attattatcc acgtcccgaa
     6361 acagggctgg acaaagaatg gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc
     6421 cttgtccaag gttttgtacc aggagataca gagttgagag gcccactata tcaaggattg
     6481 ccaactttgg aagaacatcg agggatccat cctcttgcag gcgctctaga tctattagca
     6541 agtaacacgg atattgttta catcggagat gcaggtctgt cagaaaacgt tcaggagcaa
     6601 tttgcttctt tccaaaaaga acagacagtc ttgctccata cggaacctgt agatgaagaa
     6661 ttttacgaat atatattagg gaagcatacg aatcggcaag acgatgcaag agatgtcata
     6721 cgaagcgtag atgcacgttt tcgagagatt ccgcctatcc cagcaagaaa tacagctacg
     6781 cgtatgaaag ggtctattac tttagataac gaaaagtatt tgcgctatat gggtgagatc
     6841 caattaacca aatatgattt gccagcggat gaaaaagtga atgtagtagc taaagttatc
     6901 aaagaagagc taccattgat caaccaaatc aaagctggta tgaactatca atttataaga
     6961 aaagaaggaa gataaaaatg agcaaaatca atttagagaa cctaacaacg gaacgcagaa
     7021 accaagaaac ttttggatta gatgagatga gtatttctca agccgttcaa ctaatgaaca
     7081 aagaagatca gaaagtggcc gctgcggttg aaaaagaact gacaagtatc gaaccagtga
     7141 ttgctaacac gattgagtcc tttaaaaaag gtggacgtct gatttatatg ggagccggaa
     7201 caagtggaag actaggtgtg ttagatgctg ctgaatgcgt accgacattc ggtgtagaac
     7261 cggaaatggt cataggtcta attgctggtg gagaagaagc aatgactgta gctgtcgaag
     7321 gggccgaaga ttcttatgag ctagggcaag aagatctaat caagctgaat ttgacaaaaa
     7381 atgatatagt tattggtatc gcagctagcg gtcgtacacc atatgtgatc ggtggattag
     7441 attacgcaaa atcgatcggt gctgtcacag gaacgatttc ttgcaataaa caagccgaaa
     7501 tcagtaaaca cgcggatttc ccaatcgaag tcgatgcagg tccagaattt ttgacaggct
     7561 ctactcgatt gaagtcagga acagcacaaa aacttatctt gaatatgatt tctactatca
     7621 gtatgattgg tataggtaaa gtattcaata acttgatggt cgatgtcaaa ccaacaaatg
     7681 aaaaactaat cgaacgctca aaacgaatca tcatgcaagc gacagaagct gattacgaaa
     7741 cagctgaaaa gtactttgaa gaagcagatc aaaatgttaa gctggctatc gtcatgattt
     7801 tgacaaatag tgataaagag gaagctcaag caaaattagc tgaagcaaac ggatttgtaa
     7861 aagggacctt atgaggggga aaagaccatg gcaaaaaacg aactgacttt ggaagaactg
     7921 gcaaaacaaa tttataaagg ggcaggcggc atgggcaatg tagaaagcat tgtccactgc
     7981 atgactcgtg tgcgtatgaa gatcatcaat gatgatcttg taaatgtctc tgaattaaaa
     8041 gcgattccag gagtgcttgg tgtagtagaa gatgaacagc ttcaagtcat catcggacct
     8101 gggaaagtga acaaagtagc acagcaaatg gttgatttgg caggcgtgaa attaggagaa
     8161 aaactttctt ctgtaagtac agctacagca aattcatcag catctggaaa agaccgtgta
     8221 aacgaacggg cacaagaaat gaaagcggca caaaaatcga aacaaaaacc atctaaaatc
     8281 aaatcggtat taaaagacat ttctaatatc tttgtgccaa tgattcctgc atttgtcggt
     8341 actgggatcg tggcagggat tgccgcagtt ttagcaaact tagtcacagc cggaacactt
     8401 gatgcagcaa catggcaaca atatatcgat gtgatgaaca tcttgaaaaa cgggatgttt
     8461 agctacctag ttatctatac aggtatcaat gctgcacaag tttttggtgc aacaccaact
     8521 cttggtggag ttatcggggc cgttgtgatg ctgacaggga tgaatccgga agcaccactg
     8581 aaaaacttat ttactggtga agctcttgca gctggacaag gtgggatcct aggtgtcatc
     8641 ttcgctgttt ggctattgtc aatcgttgag aaaaaactgc ataaagtagt acctgatgcg
     8701 gtagatatca tcgttacacc aactctttca ttgattgcca tcggtctgat tgaaattttc
     8761 ttcatcatgc ctttagcagg tttcatttct gatggcatgg tcggtgggat caactgggta
     8821 cttggcgttg gtggagcatt tgccggcttc gttctaggca cattattctt gccgatggtt
     8881 atgttcggac tgcatcaaat tttgactcca atccatgtgc aaatgattga tgaaactggt
     8941 cgtacgctat tgcttccaat tcttgcgatg gctggtgcag gacaagtggg tgcagcattg
     9001 gcattatgga ttcgctgcaa aaaagataaa gaattaacag aaatgatcaa aggagcttta
     9061 ccagtcggga tcctaggaat cggcgaacca ttgatttacg gagtaacatt acctctagga
     9121 cgcccattta ttactgcttg tatcggtgga ggtatcggcg gggcagtgat cggaatgatc
     9181 ggaaatgtcg gggctatcgc tattggacct tcaggagccg ctttgatccc attgatctct
     9241 gacggtaaat ggttaggcta catccttgga ttattggcag cttatgctgg tggcttcgtt
     9301 gctacattct tcttcggtat tcctaaagac cgtttagaaa aagaagaatt agcgactgaa
     9361 acagccacag taaatgaagt gcctgaaaaa gtagtacctc aaacttcagc tgaaagtgtg
     9421 actatcctct catcagttgc tgacggaaca gttgtttctt tagataaagc aagtgatcca
     9481 gtttttgcac aaaagatgat gggtgaaggc tactttgtag aaccagaaaa cggacatatc
     9541 tattctccag ttactggggt agtcagctct gtcttcccaa cgaaacacgc tattgggatc
     9601 acaacgccaa atggtttaga aatcttgctt catatgggaa tcaacacagt tgacttaggc
     9661 ggtacgccat ttgatgtgaa agtaacagaa gggcagcaag tgacacctga tacgcttgta
     9721 gcagaagctg acttagaagc gatcaaagca gcaggcaaag aaacatccat gatcgtcttg
     9781 gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa aaacaggtaa agcaaaagca
     9841 aaagcacctg tgatggatgt caaagcttaa tatgatagaa acaatagaaa cgaaagaaga
     9901 aactgaaatc tatttttcag cttcttcttt tttattattt attgttttct gctaaaatct
     9961 gataaattcg tgtcacatca gatggtgttc cccgtaattc ataatctgcc aagaaaggaa
    10021 agccaaatgt ttgtgcatat tgtttggctg ttagacaata ctgattattg aagtttttat
    10081 ttccgctacc gactacacca agacaaagtt tatgattgtc ttcatgttcc agatattcac
    10141 gcatcgtttc ggtcaagatt tctgtatcac cgttatctaa accatttccg ccgtctaaat
    10201 aagtaggaac gaaagtaaaa aatggttctg tttctttcgt aaaatcacta ttttcatgga
    10261 tttcttttaa agtaacttca ggattgacag gatcttctgc atgcattttt tcagcatatt
    10321 ctgctagatg tttggcaaag gcacgcgtat ttcctgaaat cgatatatat aaaatgttca
    10381 tggatcatcc tccaattctt tccttttagt atagcatctt ttttaagttt tttggtggtg
    10441 taatttggaa agggactagg cgctttgctt gtataatgag aaaaaagccg gtagaattat
    10501 atttctatta aagtttttat taattaagta aaaataaaaa aggtttgtta tactttttga
    10561 taaaagcggt tttattttgg aggatgctta catggataca gagcaacaga aaaaaggaat
    10621 tggtttaatt ccacttgctg ccttagtaat tggttcagcg attggaggtg gcgtatttgg
    10681 tattatgacg gacatctccg gatcggctgg cggcccagct attttcagtt gggtactcgt
    10741 aggaacagca atgttgatgc tctccttgtc gatcaataat atcaataaaa aacgtccaga
    10801 tttaaaaggc gggatcttta gttacgcaga agaaggtttt ggccgtttcg ctggttttat
    10861 cagcggttgg ggttactggc tcacatgctg gttaggcaac gtagcttttg caacattgtt
    10921 gatgagcgca ttaggatatt tcttcccagt atttgaaggt gggcaaaatc tggcatcgat
    10981 cgtgatgagt acagtctttt tatggggata tgcatggcta gtgaatcggg gagtcgaaaa
    11041 tgctagtttc gtcaatgcta tcgtcacatt ctgtaaatta gtgccattat ttttatttat
    11101 tgtagtagct atcagcacgt tttcgatcca acaattcatt gataactttt ctagcgacat
    11161 tatattgaat gcgaatggac agccgatccc gttttccagt caagtattga gttgcatcat
    11221 ggtgatgctt tgggtatttg ttggtattga aggagcctct gtcgtaggtt cacgagcaaa
    11281 aaaacaatca gatgttggga aagcaacgat tctagggata ttcggcttga ttattattta
    11341 cgtcttggtt tccatgcttc catatgggac aatgacgatg aatcagctgc aaacgatcaa
    11401 ggcgcagcct gctatgggct atgtgtttga aatgatggtt ggtaaatggg gagcagtcgt
    11461 gatcaacggc ggtttgatca tttctttagt tggtgtttgg ctctcatgga cgatcttacc
    11521 gattgaaaca atgggaaaca tggctgaaga cggacttttg cctaaagcat ggggaaaact
    11581 caacaaaaat ggggcaccaa cttatgctat cgtattgacg acgttatgta cgaatgtctt
    11641 tttactttca ttattattca ctgacagtgc ttataacttt gcttacacac ttggtacagc
    11701 agctattttc ttcacgtggc ttttcttagg attgtaccaa atgaaacttt cttatcagcg
    11761 gaaagaatgg gtccaatttt tagtagggct tttagcaagt gcctttcaag tatgggctat
    11821 gctagtcgta gcctttaatg aagtaatgct tgttcttgtt cttttcttgc caggcattta
    11881 tttttacctc aaagcacgta aggagcaaaa cgagcctgta caacatatcc acttagataa
    11941 gattttactt ataggtgtga cgatggtcgg agtgattgca atggttttat ttgccactgg
    12001 gatgcttcac gtatagaagt ataaaagaag tataaagaga ggttgtgagc ctgtcgttta
    12061 ttcaaaaaca tactaggaaa ggagctgtga cacaaatgtt gtcacagctc ctttttcaga
    12121 ataaacggtg tttaagagac agccaatcgg caataaggct gaacgtctct ttcacagcct
    12181 cttttttgat gagtcatcat acattttttc gcttaaatac gagatatcct aatgctaaga
    12241 acaaaacgat gtaagctaaa ttaccgaata gcatttgatt agtagaaaga tgagtcaatt
    12301 gcttcatgat ctcttcgttt ccaatttggt tttgcagatt caacatactg atcggattcc
    12361 atttgatcca ctcccatttt tggatgagtg caataaggat accagatatg attgacgaag
    12421 caaaataaaa gacgattcct gctgagatgg atgcgccgga actattgata aagcaagcaa
    12481 tcatcaacac taaacttaaa atcagccata aaccgatata actacccaaa gtataagtga
    12541 acaagctttg gatcagtgtt tgaccagtag aaaccttctc cgtgaaggaa atcgaaggga
    12601 aaagaatcag tttcatcaac acagtgacaa taatagctaa aaggtataaa taaacagaat
    12661 aaataactaa tgtgatccat ttgcttacta atatttctcc tcgagaatac tttcgataaa
    12721 ggagattttt caatgtacca tattgtgctt ccatcgaaat gatcgtgctt gcagctgcaa
    12781 tcatgataaa gacaatccaa gatgtggcac taaaaagctg agcagtcatt tcaactggat
    12841 caatgatcgt cgtgtatttt ttggctagaa gtgctgttcc tatgagcaaa acaactaata
    12901 gaacagataa aacagcagtt gactttttct tgatcaattt aaataattct tgttttacaa
    12961 gcaatgtcat ttttcttctt ccccctttac tgaatccaga atttccaata gagaagattc
    13021 caaatcattc gtcagatgct ggacgtctgt cacatggata ttttgtgcag acaatagctg
    13081 gatcacggtt cctaataatt cttcgttttc ttttaaaagc ttgattttat caggatcttc
    13141 caaagtatat ccagcttctg tcaagatcgt tcgagcagct agatcatttg ttgtagagat
    13201 aacgatgaaa tcttgcgctg tttggttcaa ttcagccatt gtacattgtt tgatgatctt
    13261 tccttgattg atcaggatca gctcatcgac cattttttca agctcgctga gtaagtgact
    13321 ggagatcaag aaagaggtgc cctgagtagc caagtcggta atgatttttc gcacatctct
    13381 tgttgcttgt ggatccaaac cgttcatcgg ctcatctaaa ataacgatag acggatggtt
    13441 cagtaaggca agagcaattc ccaatttttg tttcattccc aacgagtagg atttcgcttt
    13501 tttgtgaatg tacttttcca tcttgagctg attcacgata gtgtcgatag cagatacatc
    13561 gttggtttca gagaataact tcagatgatc atagcctgtt aagaatggat agatacccgg
    13621 gtattcgatc aatgctccta cttgttccaa ccctttatga ctagagggag aaatctcatt
    13681 ttcaccaata cagattgttc catctgagta atggatcagt cctaaaatag ctttcatgat
    13741 cgttgttttt ccagcgccat taggacctac taatccaacg attttgccag gggataagct
    13801 aaaactgaca tcgctgaggg cttgtttgtg accaaagttc ttggataatt cttggaccga
    13861 aagacttttc aaaaatattc actccttttc tatagatagt acaagcatac acctgatgag
    13921 tggagaagtc gctaggtcat tggacttatt tcttattttt tcataaaaaa attgaatatt
    13981 aaataaaaag gcttattttt tctgatcagt acttattcca agtgtctagt ggccgctagt
    14041 aatcttgatg ccaatgatac tgatgagtag catccccaca aagaaccaag tggaaagatt
    14101 cagcttatca tgaaacaaga ccacaccaat tatgaccgaa ccaactgcgc caatccctgt
    14161 ccagatagga taagagagac tcatcggcaa acttttgatt gcctttgaca aaaagtaaaa
    14221 actagcaatc atgccaatca ctgtgtaaaa agaataattg attttagaaa aaccatcact
    14281 ccatttcatc atcgttgacc agaatacttc caaaattcct gctatgatta attccatcca
    14341 tgccatactt acattcctcc ttaactataa aaaaatacac aaaaagagga aaatcatccc
    14401 ttcaaagacc taatggaatg atcatcctct ttatcctacc cggtggcaga ttgtatatct
    14461 ttttctgtag tatagcacac ctatccagat atcggcaatc tcatactttt tagtaaagtg
    14521 taccaagcac tgacggatat atcgttagcg gaaaagaaga tgaatgcttt gcaatttcat
    14581 agaaagaagt tgttatttat taatcattta aaaaaaacag taactagacc ttatttctat
    14641 atattatcgg gcaataaatt aaaaaaagct ttaccttaca ataagtaaga taatttttgt
    14701 gataaaaaaa taacgaaaaa tatttaaaaa tagtgtctat ttcgtgagaa aacaactata
    14761 ttttttatgt gttttgcttt ataattaaaa aaatattgtc attttatgga ggaaaatata
    14821 tggaaaatta tcttagcctt ttaggcgtct tgattgttat tctcgggttt gcatttaaat
    14881 tagactcgat attgatcgta atggttgctt tagttgttac agctttgact ggcggtcttg
    14941 gcattcaagg aatgttagaa cttttaggta caagttttgt aaacaatcgc gggatggcga
    15001 tttttatcat cattatgttg gcaacaggaa cgcttgaaag aaatggtctg aaagaatcag
    15061 cggcttcctt gatcaagcga ttcaagaaag tatctgctgg tatgattgta gatatttatg
    15121 ggatctttcg aatgatcttt gcggctttta acgtgagttt tggtggagtt gctggatttg
    15181 ttcgtccaat cattttaccg atggcattgg ggaccatcga atcaaaaggg ttagaagtcc
    15241 atcctaagca tgaagaagaa ctaaaaggta tggcttcagc tatggaaaat gtctgctggt
    15301 tctttggcca agtactattc atcggaggag caggggcgtt gctagtccaa tcaacactga
    15361 aagatctagg atatgacgta acattgggac agcttgcttt agtggaagtt ccagtggcta
    15421 tcacggcttt agttgtggca agtgtctact tctatcttaa agaaaagaaa ttagcgaaga
    15481 aatattatcc aggaaagggg caataagatg gactttttta caaatcctga tattttatta
    15541 ggcgataaat tactggagat cctttatatc gtgatgggac tcgtattgat ttacactgga
    15601 gtaagaaatt taacagataa aaccaatcct catcgttatg ggtcagcctt tttctggacg
    15661 gtcttagggg tagttattgc aggaggaaga tggcttccgg caatcgtgaa cggtggcttg
    15721 atctttgcga tgaccatacc agcaattgct aagaaagtca gcaaaggaga aagccggctt
    15781 ccatcaaaag aatatatgga aaaaatgtca gacaaactag gaatgaaaat ttttattcca
    15841 gctttgagta tcggtgtctt tgcgatcttg tttgctctat ttaccaattt aggtgcttta
    15901 gtcggcgtgg gcgtcggtgt ttttgcagcc atgctaatct tgatgttctt ttctcgagat
    15961 aatcgaccaa gtacattttt agatgatgca gcagatatgc tgggcacagt agggccgtta
    16021 agcatgttgc caatgctttt agcttctcta ggtgcggtat ttacaagcgc tggagttggg
    16081 acagtgattt ccaatgcagt aggtacgatc gttccagaag gaaatgtgaa catcggcatc
    16141 atcatttacg ctcttggcat ggtgatattt acggtaatca tggggaatgc atttgcggcc
    16201 atcaccgtga tgacggttgg gatcggtgca ccattcgtat ttagtcatgg agcgaatcca
    16261 gcgttaatag gaatggttgc attgacttgc ggtttttgtg ggaccctgct gacaccgatg
    16321 gctgctaact tcaatatcgt gcctgtagcg atgctggaga tgaaagacaa atacggagtg
    16381 atcaaaaacc agttgtttat tgcgttattt atgctagttt tccaaattgc ttatatgatt
    16441 ctatttaaat aatagaagag aggggttgtg acagaataat tgtcgcaacc tcttctaaca
    16501 ttgtagtgga acactatttt tttataagaa tatattaagt ttgatctgtt atgttattta
    16561 aactattgtt tcctttattt acgctgttca aggaaaatgg tatgataaaa gataaccata
    16621 tgttaaatca atcggacgga ggaacaacag atgaaagtac tagtaacagg ttttgatcca
    16681 tttggtgggg acaaagtgaa tccagcctat gaagcagtaa aaaaaatgcc agatgaaatc
    16741 agcggtgctg aaatcatcaa agtggaagta ccgaccgtat tcgaaaaaag cagtcaagta
    16801 gtcaaagaag ccatccaaca gcaccaacca gatgtcgtga tctgtgtcgg acaagcaggt
    16861 gggcggtcag cagtctcgtt cgaacgtgtc gcaatcaatc tagcagaagc aagaatccct
    16921 gataatgaag gaaatcagcc attcgatacg gctttagaag aaaacggtcc agccgcttac
    16981 tttacctcat tgccaatcaa agcaatgaca aaaaatgtac aagatcatgg tctaccagcg
    17041 tacatctctt atacagcagg aacatttgtc tgcaatgata tcatgtatcg tttgcttcat
    17101 ttgatcgaaa cacaattccc ggcaattcga ggtgggttca tccacgtgcc attctcaccc
    17161 gatcaagtga tcgatcgtcc agtgggtacg ccttctatgt ctttagaaga cattgcttct
    17221 tctttgactt atgcagttga agcagcagta gaaaataaag aagatatttc cggaaatgcc
    17281 ggaacaactc actaatttag caagaaacac tggctggttg ctaacgttat gttagtagcc
    17341 agctttttgt aattagaaag gaaagaaaca gccgtggaag aatggcaatc cgtctttgaa
    17401 gaatggtttc caaaagaaat cagcaaaagc tatcccatca aaatttctaa acaatacaca
    17461 agcagtcaac gttgggagat ctatgcaaaa ttaacgaaaa aacagcggga actagtcgat
    17521 aagcaccgcc gatatctaat cagctctcgt tttatggaag aacattattt agctgcaaca
    17581 gattgggtgt tttctgactt caagatcaat ccgttttttc gaacaaaacg aagccagcaa
    17641 aaattgtatt gtgagtgtgg tagagaatta aaagtgcaat acatcgttaa atcgccaaaa
    17701 accggaaaga tcctaaaact aggcatcaac cactttgccg atcacctgca tgtctctccg
    17761 acagtggcag cttcgatcca tcagggaatg acaaaagttg atctggcctt agacgaatta
    17821 ttatggctta agcagaagaa tatcgatttt cctgaaggac tatggcaaaa atactgtttt
    17881 gtcttgtacc aaaatcgtcg gatgaaacag ccatatttac cggatataaa attagctcag
    17941 cggctcgcag aatttcgcca agtagaaatg ccgatttaca tagcggatta tcaagcattg
    18001 gaaaatgaaa tcaagaaaat aagtgaacac atcaatgggc agtcaaaaaa gcgacagata
    18061 aaaaaagaac tatttgacga ctttgcagaa gaattagtaa aggatgtaga agaattcttg
    18121 atcaactatc gcgcattttt acgtaaagat tggcaatcca tcgtatatga agaagtgcca
    18181 gtacatccta atgcttattt tgaaacattt atttccgtgt tgagaaagac aaaacgtcaa
    18241 cgaacaccag aagtcactgc tcagatggaa tattttgcga aaaaccaacg attcatccaa
    18301 ccaaaaatct atctatttat ttggaagcaa tattgtcgct atggttttac agaagggttc
    18361 ttcgacagca ttcctcggat agtcagaaat ggttttttaa aagtattgcg taaagaacga
    18421 gaagctatac aatctgctga taagaaagac cgtacagtct caaaagaaaa atggcaacta
    18481 gtagtgaaag atatccaatc tggaaatgtg caagagacaa tcgacaaatg gaaaggaaaa
    18541 cattatcgtt ttactgaagc tcagaaacaa gcgttggaat attatcagaa gttagaagaa
    18601 tcactgcgtt tcaatgatga ggcaagaaag tatctgaagg aattactata aaaaacatac
    18661 taagattaga ggggaacatc attgacgaat gatgttctcc tctattttct atttttagat
    18721 tatagtgaag agtgaaagaa agttcgaaca aagccttgag gactacgagt ccctatcaaa
    18781 aaccgaactt ctttactgtc attttagaat caggtttacg tatgttgggg ccatgagccc
    18841 gtgtatagga aattggaatc gaaatgaagc agtaaagttc tttcaaaact taaaaaggag
    18901 gaaaacaaat gccttacatt cttgcattag atgtttcaat gggacacagc tacagtgtcc
    18961 tatacaaaga cgacacctgc ttatttgaag acgagattga acacactcaa agaggatttc
    19021 atgcattact cgaagagatc cagcacttgc ctgagcgtcc attgattgtt tttgaagcaa
    19081 cggggattta ttccagacca gtcgaaaagt tctttcaaga tcatcacttt tcttactgtt
    19141 tgttaaaccc gttagaggcg aaaaaacaga tggaagaaag caccttacgc agttggaaaa
    19201 cagataaatc ggatgcccac cggctcgcac agactcatct taaaaatcaa cgtcagccaa
    19261 aagaagtaca aagcaatttt tacttagaga tgcgcgattt agcccgtttt tatcaagaaa
    19321 ttgagaagaa aatcactcgt ctgcgcatgg atttacacaa ctgccttcaa ttgacttttc
    19381 cagagcttga acagttcttt tcaaacaggc tgacccctta cgcattgaca ttgatccgtc
    19441 tttttcccca tccagatttt gtattggcat ctactcggac aaaaataaaa aataaactga
    19501 tcaacgaaac acggaaaaaa atctccgcga atcgtgccga gcaaaaggca gatcaaatca
    19561 tccactatgc acagtgtgcc tatccagctg ttgaaaaaga cagtatccac tgtcaaaaaa
    19621 cgatctatta cgccgaactt ctccaagatt tactcgaaca aaaa
//
LOCUS       DAYYAO010000052        19640 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000052 DAYYAO010000000
VERSION     DAYYAO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.054"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..660
                     /locus_tag="ACHARI_001767"
     CDS             13..660
                     /locus_tag="ACHARI_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8442815.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            744..1688
                     /locus_tag="ACHARI_001768"
     CDS             744..1688
                     /locus_tag="ACHARI_001768"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8442816.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1823..3274
                     /locus_tag="ACHARI_001769"
     CDS             1823..3274
                     /locus_tag="ACHARI_001769"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8442817.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3295..4131
                     /locus_tag="ACHARI_001770"
     CDS             3295..4131
                     /locus_tag="ACHARI_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8442818.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4131..4790
                     /locus_tag="ACHARI_001771"
     CDS             4131..4790
                     /locus_tag="ACHARI_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8442819.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4916..5605
                     /locus_tag="ACHARI_001772"
     CDS             4916..5605
                     /locus_tag="ACHARI_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442820.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5611..6567
                     /locus_tag="ACHARI_001773"
     CDS             5611..6567
                     /locus_tag="ACHARI_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8442821.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6611..7123
                     /locus_tag="ACHARI_001774"
     CDS             6611..7123
                     /locus_tag="ACHARI_001774"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8442822.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7379..7927)
                     /locus_tag="ACHARI_001775"
     CDS             complement(7379..7927)
                     /locus_tag="ACHARI_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8442823.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8016..9197)
                     /locus_tag="ACHARI_001776"
     CDS             complement(8016..9197)
                     /locus_tag="ACHARI_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8442824.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9433..10941)
                     /locus_tag="ACHARI_001777"
     CDS             complement(9433..10941)
                     /locus_tag="ACHARI_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8442825.1"
                     /translation="MKKNGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11100..11291
                     /locus_tag="ACHARI_001778"
     CDS             11100..11291
                     /locus_tag="ACHARI_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442826.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11494..12411
                     /locus_tag="ACHARI_001779"
     CDS             11494..12411
                     /locus_tag="ACHARI_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8442827.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12404..13477
                     /locus_tag="ACHARI_001780"
     CDS             12404..13477
                     /locus_tag="ACHARI_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442828.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13625..14143
                     /locus_tag="ACHARI_001781"
     CDS             13625..14143
                     /locus_tag="ACHARI_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442829.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14124..14516
                     /locus_tag="ACHARI_001782"
     CDS             14124..14516
                     /locus_tag="ACHARI_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8442830.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14592..15527
                     /locus_tag="ACHARI_001783"
     CDS             14592..15527
                     /locus_tag="ACHARI_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8442831.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15660..16439
                     /locus_tag="ACHARI_001784"
     CDS             15660..16439
                     /locus_tag="ACHARI_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8442832.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16432..16749
                     /locus_tag="ACHARI_001785"
     CDS             16432..16749
                     /locus_tag="ACHARI_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8442833.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16993..18603
                     /locus_tag="ACHARI_001786"
     CDS             16993..18603
                     /locus_tag="ACHARI_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8442834.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18760..19608
                     /locus_tag="ACHARI_001787"
     CDS             18760..19608
                     /locus_tag="ACHARI_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8442835.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6343 a         3512 c         3955 g         5830 t
ORIGIN      
        1 gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta
       61 gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa
      121 cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag
      181 acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg
      241 atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga attagatagt
      301 gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca
      361 agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat
      421 gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt
      481 gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat
      541 ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt
      601 acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa
      661 gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata
      721 acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg
      781 ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac
      841 tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag
      901 gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc
      961 caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa
     1021 accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa
     1081 tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc
     1141 ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat gaaacactga
     1201 aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt
     1261 ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg
     1321 ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca
     1381 aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga
     1441 atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga
     1501 ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg
     1561 gtacagagct tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc
     1621 agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca
     1681 tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc
     1741 gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa
     1801 cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga
     1861 aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa
     1921 tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt
     1981 gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg
     2041 tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc
     2101 ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca
     2161 aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc
     2221 aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc
     2281 agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat
     2341 acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg
     2401 tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat
     2461 gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca
     2521 agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg
     2581 tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc
     2641 tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg
     2701 tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg
     2761 ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg
     2821 gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt
     2881 atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc
     2941 accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag
     3001 agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc
     3061 catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca
     3121 accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt
     3181 tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa
     3241 tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag
     3301 aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca
     3361 gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta
     3421 gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt
     3481 ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt
     3541 aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct
     3601 gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc
     3661 aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt
     3721 gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg
     3781 gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc
     3841 gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc
     3901 gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac
     3961 gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta
     4021 ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact
     4081 gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc
     4141 ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat
     4201 acacattagg gattggcttc gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga
     4261 cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt
     4321 tctttagagg cattcccgca ctagtcctgc tgtttctgtt atatttcggg ttaccttatc
     4381 aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg
     4441 gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag
     4501 cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa
     4561 tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag
     4621 caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg
     4681 attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg
     4741 aaaaagtgca acagtattta gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa
     4801 tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat
     4861 tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa
     4921 gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg
     4981 cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg
     5041 cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc
     5101 agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc
     5161 aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta
     5221 tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa
     5281 agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc
     5341 tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaaa
     5401 agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga
     5461 acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga
     5521 agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg
     5581 gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa
     5641 tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa
     5701 ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa
     5761 aaaaagcacc atccaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc
     5821 gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc
     5881 ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat
     5941 ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa
     6001 tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc
     6061 cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt
     6121 tttagtgatg actatctttt tttccacgca gacggatata ttggattttc tgatttcacg
     6181 atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta
     6241 acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac
     6301 tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc
     6361 atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac
     6421 cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg
     6481 gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt
     6541 caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag
     6601 aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat
     6661 cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca
     6721 gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat
     6781 agtggaagat caaattgctg gaacagctgc attagtctca ggcgtggatc ccgtttacac
     6841 aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc
     6901 tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac
     6961 gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc
     7021 gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt
     7081 tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag
     7141 caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta
     7201 acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt
     7261 gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt
     7321 cgtgacatag cttttgtcac gatctctcgg cttatcctac gagtattatt cgatacattt
     7381 atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca
     7441 cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa
     7501 ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca
     7561 atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata
     7621 aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt
     7681 taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact
     7741 catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat
     7801 ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa
     7861 aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat
     7921 aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc
     7981 caaagctttt aaaagtaact ttgctttttt gtattttata ccagcaagcc agtcacaata
     8041 gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca
     8101 agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag
     8161 aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg
     8221 aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc
     8281 atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg
     8341 gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca
     8401 ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca
     8461 tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct
     8521 tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga
     8581 atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt
     8641 gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg
     8701 aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat
     8761 tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt
     8821 ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga
     8881 ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca
     8941 cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga
     9001 agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat
     9061 aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt
     9121 gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata
     9181 cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt
     9241 gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc
     9301 attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt
     9361 aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata
     9421 agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat
     9481 atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg
     9541 ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt
     9601 tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga
     9661 atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg
     9721 aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc
     9781 atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat
     9841 cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc
     9901 agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat
     9961 tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata
    10021 acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa
    10081 tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat
    10141 tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc
    10201 tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac
    10261 tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa
    10321 agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt
    10381 caagcaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt
    10441 agttgtttca atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt
    10501 gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt
    10561 attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat
    10621 taataagcca taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat
    10681 cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat
    10741 ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg
    10801 aagtacacgt ttcgcatgct tgtcgtcaac taacttccca ccaaattcta aatataattt
    10861 gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga
    10921 atcaaaacca ttttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt
    10981 tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa
    11041 accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca
    11101 tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg
    11161 gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg
    11221 aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc
    11281 ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa
    11341 gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat
    11401 tgcccgctct tttttataac aattttttac agaaaatggt actattgaag tattaataag
    11461 tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat
    11521 tatgagccag acgaactaga ggagacgaac gcggatcagg atacaggaca agaatcgtct
    11581 tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta
    11641 caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat
    11701 ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg
    11761 ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa
    11821 aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg
    11881 gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg
    11941 gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca
    12001 aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat
    12061 cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa
    12121 gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt
    12181 ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca
    12241 agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat
    12301 ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc
    12361 aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga
    12421 agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt
    12481 ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac
    12541 aaaaatacat gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca
    12601 tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga
    12661 tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga
    12721 tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga
    12781 agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact
    12841 gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac
    12901 aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt
    12961 attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc
    13021 agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc
    13081 agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt
    13141 attagcccat gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa
    13201 gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat
    13261 ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta
    13321 tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac
    13381 agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta
    13441 cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat
    13501 gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa
    13561 gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt
    13621 aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg
    13681 taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat
    13741 agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag
    13801 cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt
    13861 tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa
    13921 gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac
    13981 gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt
    14041 gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt
    14101 ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg
    14161 tcagtatact cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa
    14221 aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact
    14281 tttcggaaaa agaagtggtc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa
    14341 attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg
    14401 agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca
    14461 aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt
    14521 cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga
    14581 aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag
    14641 gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg
    14701 ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat
    14761 tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag
    14821 caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg
    14881 gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca
    14941 ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa
    15001 tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa
    15061 gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg
    15121 tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga
    15181 tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat
    15241 tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag
    15301 cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa
    15361 cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact
    15421 cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata
    15481 tatacattga aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat
    15541 gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta
    15601 gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa
    15661 tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta
    15721 taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc
    15781 ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg
    15841 catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag
    15901 aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga
    15961 cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa
    16021 agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa
    16081 tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag
    16141 atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga
    16201 tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca
    16261 tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg
    16321 gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta
    16381 aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa
    16441 ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc
    16501 ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat
    16561 gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat
    16621 ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac
    16681 aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct
    16741 atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg
    16801 atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa
    16861 aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat
    16921 ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt
    16981 gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat
    17041 cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac
    17101 aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg
    17161 ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc
    17221 acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg
    17281 tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga
    17341 atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct
    17401 ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg
    17461 cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct
    17521 ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt
    17581 acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc
    17641 agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa
    17701 aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca
    17761 gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc
    17821 cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca
    17881 aataaattga cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca
    17941 atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt
    18001 ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga
    18061 ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct
    18121 ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg
    18181 aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc
    18241 caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca
    18301 ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct
    18361 ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca
    18421 gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat
    18481 gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa
    18541 ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa
    18601 taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt
    18661 gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata
    18721 aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca
    18781 gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca
    18841 atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa
    18901 tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg
    18961 gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc
    19021 catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc
    19081 agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg
    19141 gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat
    19201 atcgatcgaa tccgagataa tacctatgct tatctgattg tagatgattt gaacaattta
    19261 gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa
    19321 ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa
    19381 aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca
    19441 gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa
    19501 ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt
    19561 acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc
    19621 aaaaaacata ccaaaaatat
//
LOCUS       DAYYAO010000053        19507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000053 DAYYAO010000000
VERSION     DAYYAO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.055"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHARI_001788"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHARI_001788"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8442836.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACHARI_001789"
     CDS             complement(1173..1868)
                     /locus_tag="ACHARI_001789"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8442837.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACHARI_001790"
     CDS             complement(1881..2597)
                     /locus_tag="ACHARI_001790"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8442838.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACHARI_001791"
     CDS             2927..3532
                     /locus_tag="ACHARI_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8442839.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACHARI_001792"
     CDS             3857..4180
                     /locus_tag="ACHARI_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8442840.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACHARI_001793"
     CDS             4362..5732
                     /locus_tag="ACHARI_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8442841.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACHARI_001794"
     CDS             complement(5862..7163)
                     /locus_tag="ACHARI_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8442842.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACHARI_001795"
     CDS             complement(7393..9384)
                     /locus_tag="ACHARI_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8442843.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACHARI_001796"
     CDS             complement(9528..9794)
                     /locus_tag="ACHARI_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8442844.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHARI_001797"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHARI_001797"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8442845.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHARI_001798"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHARI_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8442846.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACHARI_001799"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACHARI_001799"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8442847.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACHARI_001800"
     CDS             complement(13954..14475)
                     /locus_tag="ACHARI_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8442848.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACHARI_001801"
     CDS             complement(14478..15320)
                     /locus_tag="ACHARI_001801"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8442849.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACHARI_001802"
     CDS             complement(15313..16038)
                     /locus_tag="ACHARI_001802"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8442850.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACHARI_001803"
     CDS             16214..16510
                     /locus_tag="ACHARI_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442851.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACHARI_001804"
     CDS             17078..18670
                     /locus_tag="ACHARI_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8442852.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACHARI_001805"
     CDS             18683..19423
                     /locus_tag="ACHARI_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8442853.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAYYAO010000054        18980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000054 DAYYAO010000000
VERSION     DAYYAO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.056"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACHARI_001806"
     CDS             complement(17..538)
                     /locus_tag="ACHARI_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8442854.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRCLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="ACHARI_001807"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="ACHARI_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8442855.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /gene="cdaA"
                     /locus_tag="ACHARI_001808"
     CDS             4618..5505
                     /gene="cdaA"
                     /locus_tag="ACHARI_001808"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8442856.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="ACHARI_001809"
     CDS             5502..6671
                     /locus_tag="ACHARI_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8442857.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /gene="glmM"
                     /locus_tag="ACHARI_001810"
     CDS             6695..8050
                     /gene="glmM"
                     /locus_tag="ACHARI_001810"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8442858.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACHARI_001811"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACHARI_001811"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8442859.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="ACHARI_001812"
     CDS             10336..10626
                     /locus_tag="ACHARI_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442860.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="ACHARI_001813"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="ACHARI_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8442861.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="ACHARI_001814"
     CDS             11667..12326
                     /locus_tag="ACHARI_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8442862.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="ACHARI_001815"
     CDS             12483..13415
                     /locus_tag="ACHARI_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442863.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /gene="tig"
                     /locus_tag="ACHARI_001816"
     CDS             13569..14858
                     /gene="tig"
                     /locus_tag="ACHARI_001816"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8442864.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="ACHARI_001817"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="ACHARI_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8442865.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /gene="yihA"
                     /locus_tag="ACHARI_001818"
     CDS             16438..17025
                     /gene="yihA"
                     /locus_tag="ACHARI_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8442866.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17099..17254)
                     /locus_tag="ACHARI_001819"
     CDS             complement(17099..17254)
                     /locus_tag="ACHARI_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8442867.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17390..18717
                     /locus_tag="ACHARI_001820"
                     /pseudo
     CDS             17390..18717
                     /locus_tag="ACHARI_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18728..18937
                     /locus_tag="ACHARI_001821"
     CDS             18728..18937
                     /locus_tag="ACHARI_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442868.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         6219 a         3380 c         4084 g         5297 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agacaccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtattgaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg agcttaactt
    17101 acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga aatcgatcga
    17161 tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg aaaagtttat
    17221 ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg tttcttttta
    17281 cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt agtttttctt
    17341 tatcattggt tataattaaa ttataaaaag atttgaaaga ggtgagaaaa tgattggcga
    17401 agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa aaaacaggaa
    17461 ataagcgaat gggctatttt gacacgtctg ttgatccagc caacatttat ccgctctttc
    17521 acacaacaag cggatccgta tgatcttcga aagcttcaag aaaaaataat gctagcatcg
    17581 gtaagagacg aaagttggtt agttgtagga aatgacgaga acgaatgtag ttttcgcttg
    17641 gaagataatc aattattgat aaaaaatgta ttgtctatct ctgttttcaa agaaaagcaa
    17701 tttttgattc gagacttcat tcagaaaaaa atgattgctc atggggtgtt cgcctatatg
    17761 cgagcttata gcgagtttat ctatcacaat acaaaacaga tctctcaacg gctattgttt
    17821 gagaaaaagg atgaaatcga acatttacct aaaatgaaac aacaaaacgg ggaagtagta
    17881 gtcgattgca accaatttcc aggatacgat cttttttacc aaggtttatg tttcacttcg
    17941 tgttgggaaa tgtattatag tcgctattat catcaaatca tccctaaacc gatttttctc
    18001 gacatccaac aagtagaaaa agtgaaggaa ttagaaaacg aagtcatatg tatccagctt
    18061 tatcgagacc cctttaactg gcaaaagcca aacaatcaaa tgtttcaatc ctattttagg
    18121 gaccagcttg ggtttgatca tcttgcttgg gacaatggtg tcggtttatt gaaaccgcca
    18181 ttcgttgaat acatttacac tgaccatacg atccaatctg tccagtatca gaatgcacaa
    18241 atgcaacctg ttcctaaaaa gaatgcttca ttttttgtaa caaaaagtta tgatatccaa
    18301 caaggagatt ataaagaaag gcgtgttcgc ggaacactaa atgctcaggc gtattttcct
    18361 tgggttgacg agaatcgatc acgcatgatg tgctataaag ttatcgatcc gactgttgct
    18421 ttggataatg gaatcgaagc ttattgttat tatattaggg aatatctaga agtagaggta
    18481 acagatgaaa aatatcaaga gtatctttta tccttaagaa tctatgttcc ttcagaatcc
    18541 ctgtcagaat taccgttaaa ggaaatcaaa catagactct ctgatgttac cttcaaacga
    18601 ataaagaaaa agcggcgaag tatccaattc gatgtcaaga aaggtgagca gcatctgcgt
    18661 gtgcaatttt tagattaccg agaattagaa caattgatta ctttacaaaa aatatgaggt
    18721 gaagaaaatg gataaacaac tctattatcg aataatggtt ccggcatcat tattatccgt
    18781 ctgtctcttt tttgttccta attttcctat tcgatcagtc atcggttttt tggtcatgtt
    18841 tggcgggatg ggactttatt attatcttct tcataaggaa agaaaacctg cttctgttta
    18901 tatgaaaaaa acaagaagcg aaaataaaaa acgataaaac ggcttgatga tagacaaaaa
    18961 aataaaaaac cagtctttct
//
LOCUS       DAYYAO010000055        18119 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000055 DAYYAO010000000
VERSION     DAYYAO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.058"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACHARI_001822"
     CDS             133..636
                     /locus_tag="ACHARI_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8442869.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACHARI_001823"
     CDS             763..1800
                     /locus_tag="ACHARI_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFM8442870.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACHARI_001824"
     CDS             1815..3512
                     /locus_tag="ACHARI_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFM8442871.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACHARI_001825"
     CDS             3505..3966
                     /locus_tag="ACHARI_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFM8442872.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACHARI_001826"
     CDS             3979..4839
                     /locus_tag="ACHARI_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442873.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACHARI_001827"
     CDS             4877..5266
                     /locus_tag="ACHARI_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8442874.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACHARI_001828"
     CDS             5253..7700
                     /locus_tag="ACHARI_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8442875.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACHARI_001829"
     CDS             7705..9813
                     /locus_tag="ACHARI_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8442876.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACHARI_001830"
     CDS             9810..10814
                     /locus_tag="ACHARI_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8442877.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="ACHARI_001831"
     CDS             10833..11744
                     /locus_tag="ACHARI_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8442878.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="ACHARI_001832"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="ACHARI_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="ACHARI_001833"
     CDS             complement(12406..12621)
                     /locus_tag="ACHARI_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442879.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13089)
                     /locus_tag="ACHARI_001834"
                     /pseudo
     CDS             complement(12880..13089)
                     /locus_tag="ACHARI_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13392..14831)
                     /locus_tag="ACHARI_001835"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="ACHARI_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="ACHARI_001836"
     CDS             complement(15379..16050)
                     /locus_tag="ACHARI_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8442880.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="ACHARI_001837"
     CDS             complement(16399..17850)
                     /locus_tag="ACHARI_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8442881.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(17962..>18119)
                     /locus_tag="ACHARI_001838"
     CDS             complement(17962..>18119)
                     /locus_tag="ACHARI_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFM8442882.1"
                     /translation="DLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAV
                     TGLNIET"
BASE COUNT         5909 a         3036 c         3494 g         5680 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttagtaacg attatgtttc aatgtttaat cctgttactg cctttaaaat
    18001 aaaattatag ggttggctaa aactaggatg aaataaataa tctaacattt tcaatttgtc
    18061 taatgtaatt tgttcttcta tagcaagaga aaacattgat aatagtccag ataaatctt
//
LOCUS       DAYYAO010000056        17151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000056 DAYYAO010000000
VERSION     DAYYAO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.059"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..556)
                     /locus_tag="ACHARI_001839"
                     /pseudo
     CDS             complement(270..556)
                     /locus_tag="ACHARI_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(733..1911)
                     /locus_tag="ACHARI_001840"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="ACHARI_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="ACHARI_001841"
     CDS             1941..2351
                     /locus_tag="ACHARI_001841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442883.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2432..3052
                     /locus_tag="ACHARI_001842"
     CDS             2432..3052
                     /locus_tag="ACHARI_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442884.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3753..4037
                     /locus_tag="ACHARI_001843"
     CDS             3753..4037
                     /locus_tag="ACHARI_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442885.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            4040..4957
                     /locus_tag="ACHARI_001844"
     CDS             4040..4957
                     /locus_tag="ACHARI_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8442886.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4950..5732
                     /locus_tag="ACHARI_001845"
     CDS             4950..5732
                     /locus_tag="ACHARI_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8442887.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5733..6413
                     /locus_tag="ACHARI_001846"
     CDS             5733..6413
                     /locus_tag="ACHARI_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8442888.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            6406..7302
                     /locus_tag="ACHARI_001847"
     CDS             6406..7302
                     /locus_tag="ACHARI_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8442889.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            7497..9146
                     /locus_tag="ACHARI_001848"
     CDS             7497..9146
                     /locus_tag="ACHARI_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8442890.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            9740..10675
                     /locus_tag="ACHARI_001849"
     CDS             9740..10675
                     /locus_tag="ACHARI_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8442891.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            10668..11438
                     /locus_tag="ACHARI_001850"
     CDS             10668..11438
                     /locus_tag="ACHARI_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8442892.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            11854..12837
                     /locus_tag="ACHARI_001851"
     CDS             11854..12837
                     /locus_tag="ACHARI_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8442893.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            13284..14321
                     /locus_tag="ACHARI_001852"
     CDS             13284..14321
                     /locus_tag="ACHARI_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8442894.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(14410..14733)
                     /locus_tag="ACHARI_001853"
     CDS             complement(14410..14733)
                     /locus_tag="ACHARI_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8442895.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(14720..15427)
                     /locus_tag="ACHARI_001854"
     CDS             complement(14720..15427)
                     /locus_tag="ACHARI_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8442896.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            15513..15854
                     /locus_tag="ACHARI_001855"
     CDS             15513..15854
                     /locus_tag="ACHARI_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFM8442897.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(16025..16873)
                     /locus_tag="ACHARI_001856"
     CDS             complement(16025..16873)
                     /locus_tag="ACHARI_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8442898.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         5462 a         2916 c         3574 g         5199 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc
     2401 aatttattca gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat
     2461 aggagctaaa aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa
     2521 ctggtttttt tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa
     2581 aggaatcggc ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc
     2641 tgaacaaaca tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa
     2701 aaatgtaact gcgcgaatta gaaaagaaaa tgaagtagac attgtttttg gaattgacca
     2761 tgaatgtaat tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg
     2821 ttgcaaggac gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc
     2881 cttatatatg tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa
     2941 aataggtatt gaagtaattg aatcgattat gtgtcataat catattacgc tagaagaatt
     3001 agaaattttg atagggcaaa aaaataataa cacgaaaata caagtttctt agaaatcatt
     3061 ttttattgag atatctagat aaatgattta ttcttcaatc aaccaactcg ccaagctgag
     3121 gtaatgtttt gaggtaatga taacatacaa ctttttatac cctagtaaaa ctctataaac
     3181 aaagacttat taatttcgca tgaaatacat tcctgtaaaa tgcaataaaa tagagttttg
     3241 tttaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat
     3301 tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt
     3361 accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt
     3421 tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg
     3481 gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag
     3541 tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata
     3601 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt
     3661 gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt
     3721 attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct
     3781 ggcttcagcg agaagttcca acgcatatac aaggacgtct gatgtcaacg ataacaacga
     3841 ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggcaga
     3901 caactcaacc acgtctgcga gacagtctga tttttcttga aattcaattg gaaaatttac
     3961 actttcttta tacatttctg gctgctttct ttatacccgt caactatact tcacttatca
     4021 ataagaaaga aggataagta tggattttgg aaatgaaacg gtcttgaaag caactggtat
     4081 aaccaaaaaa tacggtgctg ccaaagcatt agataaagta tcgatcgaaa tcaaacgtgg
     4141 aatgatctat ggtttgattg gggagaatgg agcgggtaag tctaccttta tgagaactat
     4201 tatgggtttg atctcgattg atgaaggttc gatcgaatta tttggaacga cggatttgca
     4261 ggcagcaaga cgacgtatgg gtcaatcgat tgaaacccct gctctttatc cagaattaac
     4321 tgcaagagat aacctacgta tccaagcagc gaacggcggt gtgtcggata gagaaatcga
     4381 agacctgctg aaaatgatgc gattggaaaa tacagggaaa aagaaagcaa agaatttctc
     4441 tttaggtatg cgccagcgtt tagctattgc aaatgcactg atcacgaatc cagaattttt
     4501 gattttggat gaaccgacga atgggatgga tccagcaggg atggcagaaa tgcgagaaat
     4561 catccaacgt ttggtaaaag aacggggcat caccgtcttg ctttctagcc acttgctaga
     4621 tgaattgtca cagatcgcca ctcactacgg tattttgcat gaaggacact tgatcaaaga
     4681 attatcgaaa gaagaattgg cacaagaatc tcgtcaattt ataaaaattg acacttcggc
     4741 gacggaacaa gcagtaacgg ttctcgactc tcttggttat agggattatt ttgtccaatc
     4801 tagccgtgtt atccagcttt ttgaaggaat tgaccaagta gcagccatca atcaagcatt
     4861 ggtagaggca aaagttcctg tggatggtat ccacctagtt gggcaaaaac tggaagatta
     4921 tttcttacaa ttaactggag gcaatccaca tgtttaatct attaactgct gaaaaaataa
     4981 aagtatggaa gagccgtaaa atgtgggtct cactaggact gatggtcgta cttccgcttt
     5041 tttattcttg gaacgaatgg tatatgcata caaaatacgg caatgaatta aaccaagcaa
     5101 cggatacagt catcaacggt gcaacaggga ttttaatggt ggaaaaaatg gctgcatgga
     5161 ttcttctggc tttcgcagca tttgcgtgct tttatattgg ggaagaattt caaaacggta
     5221 cgattcgcaa tacgttatct ttaggacgga atcgaatgac ttattatgtg tcgaaacttc
     5281 tcgttacgct tttgatcaca acacttggcg tggttttgat tacggggcta gccatgattg
     5341 cttacacact tgctttcgga tttggtgaag ttgagggaat caaaaactat ggaaattatg
     5401 ttctaaaagt atttcctgta ttgcttctgc tgatattagc gacattatct gttccagtgg
     5461 ctttgacatt catcactaga agtaccagtg tcagtttgct tctttctttt ctttatatca
     5521 tgggaacggc cttcgttccc ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg
     5581 agtggtttac ggaaacgtgg ctcatgtata cggactttgc gcaacaagca acttattcgc
     5641 aagcaccgaa aatgattctg gtcagtctag taacaatcgt ggtatcatca gctctaggaa
     5701 tgtttatttt ccaaaaatct gacattaagt aggtgttcct gctggaaact attttgatta
     5761 tcgaagacga tgaagctgtt cattctttac tggaggaggt tcttgaacag agatacaaac
     5821 ttcttgatgc ttattcaggt acggaaggaa aattattgtt aagcacatat cctgtcgatt
     5881 tgatccttct agatctcatg cttccagggc tttcaggtga agcattgtta gcagaaatcc
     5941 gacagacatc gaatgttccg atcatcgttc tttcagcaaa aagcgatcaa agagataaag
     6001 tttccttgtt ggcagcagga gcggatgact atgtgacgaa accatttgat atcgaagaac
     6061 tgttgttacg aatcggcatc caacttcgtc atcagacttc tgtccaagta aaagatctgt
     6121 cgcaacgcat ctattacaag gaaatcttac tggacaaaga gtcgagggat gtaaatgtga
     6181 atggtgtggc tgtccatttg acaggaagag aattcgatct gctgaagctt tttttagaac
     6241 atccgaaaaa agtattttcg cgagcaaatc tttatgaaac agtttggcaa gaaccatatt
     6301 ttgatagtga gaaaacagtg aatatgcata tttccaatct tcgagggaaa ctagcaaagg
     6361 cacatgctgt gtatattcat acggtgtggg gagtggggtt tagatttgat taagtggctg
     6421 ttgcttctcg ttgttatctt tatgggaagt tttattttct tcctgcaaag ggaaatcaag
     6481 agattaacta agaaaatcgc cactctttct gaacgacaag aatatggtgg tcgtctttca
     6541 gtcgaatttc gagaaaagaa tctaatggac ctagtagatg cactgaatac atgggtggat
     6601 gaaaatgaac aaaaaggaca agcatttcaa gaaatggaag aaaacatcag actatcgatc
     6661 gtcggccttt ctcatgatct cagaactcct ttgactgcta ttaacggtta tgtacaatta
     6721 ctggaacagg tagaggatac ggagaaacgg gagaagtatt taacgatcat caaacaatct
     6781 gttgcaagat tactcagtat gacggatcag ttttatgatc ttgctcgaat agagacgaag
     6841 caaaaagaaa tggatctcca tacgctttct ttttctaaag aggtggaaga agtatttttc
     6901 tctttttatg aacaattttc tgagaaaaat atcgaggtag cgttcgctga acactcgata
     6961 aatacaactg tattagcaga tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag
     7021 aatttattgc gttatgcaga tggacaagca aaggtgacat tttcaaaaga ggattcattc
     7081 attcgattaa cagtggccaa tcctatcaaa ccaggagcgc aaacctctgt tgaaaaagtc
     7141 ttccagcgtt tttataccga aaataccagc cgcacaaata cggaatctag cggaatcggg
     7201 ctttacctgt caaaaaaatt ggtggaagga atgggaggag agatgaccgc tagattagac
     7261 gggggcatct tctccatctc cgtcaagttg cggagggttt aattggctct aatttacctg
     7321 aagtgaatga caaaaaattg tatctcgttt taaagacatc ttcttttttg gaaggtgttt
     7381 ttattttggt gtgattgata cgaattaggt aacgaagaca gttatttttg tacccccttt
     7441 tcaaatagcg caacagtgct atactaaaac taacaaatca aggaacggag agggatatgg
     7501 gccaaagaga atttatcgtt ttaattatta ttgcagttgt tattttatta gttgttttag
     7561 acattttttc acgtttgaaa gtaaaggaaa cagttcgtag taattgggga aaaattcctt
     7621 atcaaccgag atttgataaa gaagaaagtt tgaaggatgc gtggctcacg gagaaaaaat
     7681 tccgttcatg ggatagtgaa atcgacgatc ttacttggta tgacttggat atgttcgaag
     7741 tatttgaagg gatcaacagt acctattcta gtgtaggttc agaagcgttg tatcagcgtt
     7801 tacgtagctt tgattttgga gaagattatc agttagaaaa actaattgct ttttatcagg
     7861 aaaatcctca actgagagaa agaatccaat atcaattcgc tcgtttagga aaaaaagatc
     7921 ataattttgc aaaacaatat ctagcggatg gaaaaagtaa aaagattgga cgcgtaggtt
     7981 tatttatttt tcttggcttg cttccgctgg tgggtgtagg tttgcttctt ttagggcaag
     8041 tactaggaat ctatctagca atcggtggct tagtattcaa tacggtctat tatctagtga
     8101 agaaacaaac actagaaaca gagctgaata gcatgagtta tttggtttca acgatttcag
     8161 ccgccaatca gttagcaaaa atgacgacac cacttcaaaa agagctgaaa cagcaaacta
     8221 aaccattaag agacatccct aaattaggtt tttcatttcg aatgaagagc gggagtgaag
     8281 cagaattgat ttttgaatat gtcaacatca tgttcatgct gccatttatc tcctatcatt
     8341 tcgttctcac taaattagaa aagcattcaa ctgaagccat caagctgtgg gaattgctgg
     8401 gagaattgga agttgcagca gccgttttga attatcgcac ctatatgccg atcacttgcc
     8461 gaccagagtt tactgagggc ggtgtgacag cgaaagatat ctatcatcca ttgttaaaag
     8521 aagcggtagt taatccagtc aactggcaga agaatacatt agtcacgggt tccaatgcat
     8581 caggaaaatc tacgtatgtc aaaagtatag ccatcagctg tattctagca cagacgatcc
     8641 aaacagcgat tgcagaacag tttaccctgc aacctggaca tgtgttgact tccatggcaa
     8701 tcgaagatga tttatttgaa ggcgacagtt attttgtttc agaaatcaaa tcgatcaaac
     8761 gtttgctgga ccaagtggct tccaaagaac gctgctactg cttcgtagat gagattttaa
     8821 aaggaacaaa tacgatcgaa cggattgctt cttccgcttc agtcgttcgg tggcttgcgg
     8881 agtatccatc tttagcattt gtcgccacac atgatatcga attgacagag attttaaaga
     8941 attattgtga gaatgtccac tttgaagaac aagtaacaga aggaaaagga atcagttttg
     9001 atttcaaatt aaaagaagga ccttcaagga cccgaaatgc gattgccttg ctgaaagttt
     9061 tgaattatcc taaatccata gttgaacagg cccaaaaaga aagtttgttg ttcgatgaac
     9121 agcgtcagtg gtatccgttt gattaacttt atcaaacgaa gcatgatgcg tgggtccaaa
     9181 aagacgatta tcaaaagaag tacattgatg ccaaaaaagt ggcagaattc aatgggaacg
     9241 agtctgctgt ccagatgatc ttggaagatt tagcaaaaga cgatacaaaa taaaccacgc
     9301 tgtcttcctt ctagcagctt ttatcgaaaa aacttagcat cagcgacgag acgcacgttc
     9361 catggcaaag ttctccgtaa aacaaacagc gaaaacagaa attgaaaata actatttcca
     9421 ttttatttat ttcggttaaa ataaaagaaa aaagctttcg ataaaaggac ggaggaaagc
     9481 ttaacatgaa taaaaaagac aagaacctca gttataagga tcttctatat gtcatcgtag
     9541 tagttgctgt cggtactttt atgattttaa aagttccaga gttgtttaga tagttattct
     9601 atcgaagatg atcatgaact agaaaacaca tctcctcttt gttagcaggt gtgtttttgt
     9661 gtggaagcca agttgttttt tcatctgctg tattccatga tacaattaag aatcatgtct
     9721 ataaggagat aggaaataaa tggcaaatgc gcttgaaata aataatttga aaaagatcta
     9781 tggaagtggg gtcgaagcat tgcggggaat cgatctttcg gtgaaagaag gagatttcta
     9841 tgcgctatta gggccaaatg gagctgggaa gtctacaacg atcgggatta ttacttcttt
     9901 agtcaataaa acatctggaa aagtgaatgt cttcggttat gatctggaca aggatctagt
     9961 tcgagcaaag caacagattg gattggttcc acaagagttc aattttaacc cttttgaaac
    10021 ggtccagcaa atcgtcgtga atcaagcagg ctactacggt gtctctcgca aagaagcgat
    10081 gaaacgcagc gaaaaatatt tgaagcagtc caatctttgg gaaaagagaa atgtccgtgc
    10141 acgtatgctt tccgggggaa tgaagcgaag actgatgatt gctagggcat tgatgcatga
    10201 accacgtttg ctgatcttgg atgaaccaac agcaggagtt gacatcgagt tgagacgcga
    10261 gatgtgggag tttctacgag aattgaacga aaatggtaca acgatcattt tgacgacgca
    10321 ttatctagaa gaagctgaaa tgctttgtcg caacatcgga atcatccaat cgggtgaact
    10381 gatcgagaat acaagtatga aagagcttct gtcaaaactg caatttgaaa catttatctt
    10441 tgatttagcg ccatatgata ccaaacctgt gattgaagga tatgcttgta catttgaaga
    10501 tgagcagaca atcgctgtcg aagttgaacg aaatcaaggg gtgaacggaa tcttcgatca
    10561 gcttacaaga caaggaatca aagttctttc tatgaggaat aagtcgaatc gtttagaaga
    10621 actgttcttg aaaatcacag aagaaaataa tcatacggag gagaaaaatg tttaatcttt
    10681 attttactgc attaaaaagt ttagctatca aggaaacgaa ccgctatttg cgtatatggg
    10741 tccaaacact ggttccgcca gtgatcacga catccttgta ttttgttatt ttcggaaaaa
    10801 tgatcggtgg gcggatcggt gacatgggtg gtttttcata tatggaattc atcgttcctg
    10861 gtttgattat gatgtctgct atcactagtt cgtatgccaa tgtttcttcg tccttcttct
    10921 ctcaaaaatt tcagaaaaat atcgaagaaa tcttggtagc acctgttcct acgcatgtga
    10981 ttatttgggg ctttgtgatt ggtggtgtgg gtcgcagtat tctcgtcggc tctctcgtaa
    11041 cgatcatttc cttgttcttt gtaccacttc atgtgtattc atggttcatg gtcatcatta
    11101 cattattgat gacggcaatc ttgttttcat tagcaggact gctcaacggg atttttgctc
    11161 agtcttatga tgatgtatcg atcgtgccta catttgtctt gcagccgtta acttatctag
    11221 gtggcgtttt ctatgccatc tctatgttac cgcccttttg gcaagctgtt tcaaaagtca
    11281 atccgatcgt ttatatgatc tccggtttcc gttacggatt ccttggaacg atcgatgtgc
    11341 cagtagcaac atcgatgatc gtacttgtgt tattcatcgt tgttttgtac gccgttactt
    11401 ggtatttgat caataaagga agaggattga gaagttagaa aagaggttgt gaaatcagcg
    11461 ttttgacctg agtattagag aaacaaacgg gaaaatagtt cctcatattt tgttgttttt
    11521 tgagcgaata ccgagggtca gctgattgaa caccgtttag tggagaggtt gtgaaaaagc
    11581 tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg
    11641 gttggactta ttaccgcaga tgcaagcttt tgaacaccat ttattccgga aaatggactg
    11701 tgataagatt tttgtcacag tctttttttt gctcaatcag aaacatcttt taagctgttc
    11761 actttgcttc tttttttgtt tcctagtata atgacaatgt ttttgagaaa gattgcgact
    11821 tcactgcttg atagaatgga gagtaacaaa taaatgacta ctaatttttg gaaagagctg
    11881 ccaaagcctt tttttgtttt agcaccaatg gaagatgtaa cagatgttgt atttcgccat
    11941 gtcgtaaagc atgcagcagc accggatgtc ttctttactg agtttacaaa ctcagacagt
    12001 ttctgtcatc cagatggaaa agacagtgta cgaggaagat taacatttac agaagatgaa
    12061 cagccgatgg ttgcccatat ttggggagac aagccagaat tttttagaga aatgagcatt
    12121 gctatggcag aaatgggctt ccaaggaatc gatatcaaca tgggctgccc agtccctaat
    12181 gtagcagaac gtggaaaagg cagtggcttg atccttcgtc cagaagtagc ggcacaattg
    12241 attgaagcag ctaaagctgg cggtttgcct gtcagcgtca agacgcgtat cggctatacg
    12301 gaaatgtctg agatggaaga atggatcagt cacctacttc atcaagatat agccaatctt
    12361 tctgtccatt tgcgtacgcg taaagagatg agtaaagtag atgcacattg ggaagtcatt
    12421 cctaaaatca ttgctttacg tgaccaaatc gctccacaaa cactaatcac gatcaatgga
    12481 gatatccccg atcgccagac aggagaagaa ttggccgaga aatatggcgt tgatggcatc
    12541 atgattggcc gagggatttt caagaatcct tatgcatttg aaaaagaccc gagggaacat
    12601 acagaaaaag aactgatcgg actacttcgt ctgcaactag atctgcaaga tcattattca
    12661 gaaatcatcc cgcgttccat cacaggacta catcgtttct ttaaaatcta tatcaaagga
    12721 tttccaggag caaatgactt gcgagttcag ttgatgaata cgaaatctac tgatgaagta
    12781 cgagagatct tagagacctt tgaaaaagaa catccaacgc ttttcagtga acaatgattt
    12841 cttgcttgaa caccgtgaat tagaggtcgt gaaaaagctg tcctgtatca agtaataaga
    12901 acagccaaac aaaaatagct cttcatattt tgtcgttttt tgagctaata ccgagggtca
    12961 gttgattgaa caccgtttat atcagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    13021 ggagcaattt caaaaaacag cttctcatgt ttgatgaata ttttgacttg tgaccgaaga
    13081 gttggtagga gaacaccgtt tataagaggt tgtgacaaaa gtcttgtcgc aacctctttt
    13141 ttaaccaaaa aagtgcaaat agtttacttt aagtaagaga ataattatga tgaataaaaa
    13201 aggtggaaca aatagcatac tatatatatt gaaatgaaca ctgtatggat gctccaactt
    13261 gatgaagcta aggaggaatt cgtatgtcag aaaaggaatt aatgaaggta gtcggttttt
    13321 atgaagggtt gccgatcgat gatccaaaaa gctttattga tgaaaaagaa cagatacctg
    13381 tacccagttc acgggatctt ctggtaaaag tcaacgcagt atctgttaat cctgtcgata
    13441 cgaaacttcg ccaagataac gggataagaa atgctttacg tatcttaggt tttgatggag
    13501 tcgggaaagt tgttgctgta ggtgacgaag tgcaaaaatt ttctgtcggc gatcgcgttt
    13561 tttacgcagg tacaacaaca agagcaggaa gcaatcaaga ataccaacta gttgacgaac
    13621 gtattgtcgc tcttgcacca aaaaatcttt cagatgaaga agctgcagcc ttgccgttga
    13681 cctcattgac cgcttatgaa ttgttatttg aaaaatttgg tctaactcca gaagaaaatg
    13741 ccaatcgcgg gaaaaaaatc ttagtgatca acggatccgg gggcgtcggt tcgattttga
    13801 atcaactagc gcattgggct ggcctagaag tctacgcgac tgcaagtccg aagaattttg
    13861 aatggctgaa aaaaacaggt gtagactatc caatcgatta tcatcacgac ttaagtaaaa
    13921 gttttcgtga attaggaatc gatcaagtag agtatgtcgc tgtattattc gatattaccc
    13981 gctactttga tcaaatcaag caccttgtcc gaccatttgg acatgtggga acgatcgtag
    14041 ggatcaaaga gccgttagat ataggtgttt ggaaaaacca atctatcagc ttcgactggg
    14101 agtatatgtt cgctaaaaca gactataact atgaaatcga aacgcaagga gaagctttag
    14161 cgcacattgc tgctttagca aatgaaggga ttatccgcag tacattgagc aaggtctatt
    14221 cagatgggat gaatgcgaag aacttgaaac aagcaaccaa agacgtagaa acaggacata
    14281 tgttaggaaa agtcgttgtt agtggtccgt tcaacggata agcaagaata cgccgatttt
    14341 tttcttattt gactttgata aaaacaagag cgcatgacag taatttgcca taagctcttg
    14401 tttttttatt tataataagc gaatcaacga aagggaaaga attcctgcca agacgatcac
    14461 caataagctt tttgaaagaa tagcagctaa aagtgtagga actgaagcca aagcattttc
    14521 gagattgatc tgcggcagat gtccgatatt ttgggtaaat agactgctga accataaagc
    14581 agacataatg acgatcggga caaaacttaa atactcaagc aacggttgcg gtaaatcgaa
    14641 ttttttcagt aagacaaatg gtaggaccct agaaagccaa gttgctaaag tacaacctaa
    14701 aatagtcagc aatgtaaact caaaagaagg catgtttgat ccacaccccc aatccgcacc
    14761 caatcagtgt tacaactaca atcactaagt tgcttgggat aaaaatcagt ccgacatata
    14821 ccagaatcaa agtgagacca atcaggatca actgtaatcg tttagaggtg tttctatcag
    14881 aaatgacttg aagataaagt aacccgataa acatagcgac gatggcgaaa tccaaaccaa
    14941 actttttcgg atcagtaata aaattcccta aaagtgcgcc aacaagagaa gaacccaccc
    15001 acgtcaaata agaaatccaa ttagctgtat tcatccaacg aaagtttaac ttttgtccag
    15061 tatagttcaa tttattcatt cccagtgcga aactttcatc tgtcagcaat gtgccaatca
    15121 gcagattttg aagcagccga tttttcttga aataaggagc aatcgtcata ctcatcagaa
    15181 tcattcggga attgactaga aaggtcgaga aaacgatgga aagcaatggg ctgtgactag
    15241 caagcatgct gactgtgata aattgggcag aaccagcata gaccagcagg gacatcaacg
    15301 tgacgactag tggatgaaat ccggcagctt tcccaacgat accaaatgca agtccaatac
    15361 cgatataacc gaaaacggta ggtaacgtgt ctttgatcgc agtttttata tctaactttt
    15421 catccaataa caaaactcct ttagcacgac ataaattttc ataattgtcg ttaaacttgt
    15481 gaatattcta aatttttaat gagcatttgt caatggtggg attgaaacgt aaagaactag
    15541 cgggtcgcag atttacacgt attgtttcgg tgggaggagg cgcgaaaaat cttgcatggc
    15601 tacaaatcca agctgatatt ttcgatgcaa cggtctattg tttaagtgtg gaggaaggac
    15661 ctgcattagg cgcaacaatg attgctgcac tagggttagg gtggttcgac acacctgaaa
    15721 gttgttgtca gaattttatc caatatacag atgcagtgga acctctctta caaaatgtag
    15781 aaaagtatca taaaatctat caacaatgcc ggaaaatcta tccagcaacg aaggaaattt
    15841 gtcatgactt ataaaacaaa acgcgtacct aatagtcaaa tgactaatat tgggtacgcg
    15901 ttaagtcttc tttacttcat cctttgcatg attcgtgttt ttaaagcaag gtctgctgta
    15961 tttcttgtgg atttaggatg gttgttatcg tttacttgct atttttcatt gaaatcgtct
    16021 ttctttaccg tttctttagt tttttatcta tttttttctt ttggttagaa aattcttttt
    16081 cccgaaagtc ttctttcagg atttttatag agtagcgttt tgaataagag gcgtcagtat
    16141 gatcgacttg gcagacgaac caagagaaca tttgcctttt ttctgcatga ggtgcttctg
    16201 ctaataattc tttccataaa taataacagt cctctatcag aagaagggtt cgttcatcct
    16261 caggctccca atagaatgtt tcgagagtag caatcacttc attgatcaat tcgaatgcgg
    16321 gaagataggc attttgctga ataagcgatt caactgcttc ttggatgaac tttcgcatct
    16381 ccatgatgaa cttcgctgtt ttcctgtttt tgtttttctt gataatatga tcgtatttat
    16441 ggatgatagt tgttaattta tctgaataat ttattgcatg aaatggcatc ttaactttga
    16501 agcgttcgat aaagtgcgga tgttctataa gaatctctgt taaaagctcc cgtacaagag
    16561 aggaactagc ggaggaaaca agggacgaga gttcggaaag tgagtagttg gattttttta
    16621 ttggacgttc ttcgtaagcg aataagacag cagccatgtg tttgcatttt tttccttcta
    16681 tggcatatag acaatcacaa gtcatgttgg tcttttcatc ccaagtgatc actaccttat
    16741 aatcttccgt gccagaaact gttgcggaca agtacttgct tgtatagttc aaatgacgga
    16801 caaaaccgtg cagatgatag ctgtaaccgc gttccaaaac gttttcagga aaatactcaa
    16861 gccaattatt catctagttc tcctttcatg aaacacttat ttatatttag tataaagaaa
    16921 cgtttaagaa aaaagtagct gattttacat atttgataat cacttttgct cgaattgtca
    16981 aaaattcatt ctccttttct tttcttagag aatgtgagaa aaagtaaaag aatagcgtgt
    17041 tttaaagatg gaactttttt tgttttttat ttatctgaca aaaatgaagt gattttgtta
    17101 gaaataaata attgttccaa ttgataaaag aggtatgatg tatcgcaaag a
//
LOCUS       DAYYAO010000057        16768 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000057 DAYYAO010000000
VERSION     DAYYAO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.060"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /locus_tag="ACHARI_001857"
     CDS             complement(<1..302)
                     /locus_tag="ACHARI_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8442899.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKS"
     gene            complement(304..501)
                     /locus_tag="ACHARI_001858"
     CDS             complement(304..501)
                     /locus_tag="ACHARI_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8442900.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(895..2622)
                     /locus_tag="ACHARI_001859"
     CDS             complement(895..2622)
                     /locus_tag="ACHARI_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8442901.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2615..3808)
                     /locus_tag="ACHARI_001860"
     CDS             complement(2615..3808)
                     /locus_tag="ACHARI_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8442902.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3995..4639)
                     /locus_tag="ACHARI_001861"
     CDS             complement(3995..4639)
                     /locus_tag="ACHARI_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8442903.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4733..4867)
                     /locus_tag="ACHARI_001862"
     CDS             complement(4733..4867)
                     /locus_tag="ACHARI_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8442904.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4869..7001)
                     /gene="feoB"
                     /locus_tag="ACHARI_001863"
     CDS             complement(4869..7001)
                     /gene="feoB"
                     /locus_tag="ACHARI_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8442905.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6998..7471)
                     /locus_tag="ACHARI_001864"
     CDS             complement(6998..7471)
                     /locus_tag="ACHARI_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8442906.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7872..8096
                     /gene="nrdH"
                     /locus_tag="ACHARI_001865"
     CDS             7872..8096
                     /gene="nrdH"
                     /locus_tag="ACHARI_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8442907.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8255..10414
                     /gene="nrdE"
                     /locus_tag="ACHARI_001866"
     CDS             8255..10414
                     /gene="nrdE"
                     /locus_tag="ACHARI_001866"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8442908.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10464..11429
                     /gene="nrdF"
                     /locus_tag="ACHARI_001867"
     CDS             10464..11429
                     /gene="nrdF"
                     /locus_tag="ACHARI_001867"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8442909.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11518..12657)
                     /locus_tag="ACHARI_001868"
     CDS             complement(11518..12657)
                     /locus_tag="ACHARI_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8442910.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12966..13679)
                     /locus_tag="ACHARI_001869"
     CDS             complement(12966..13679)
                     /locus_tag="ACHARI_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8442911.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13933..14634)
                     /gene="nagB"
                     /locus_tag="ACHARI_001870"
     CDS             complement(13933..14634)
                     /gene="nagB"
                     /locus_tag="ACHARI_001870"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8442912.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14868..16400
                     /locus_tag="ACHARI_001871"
     CDS             14868..16400
                     /locus_tag="ACHARI_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8442913.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16591..>16768)
                     /locus_tag="ACHARI_001872"
     CDS             complement(16591..>16768)
                     /locus_tag="ACHARI_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8442914.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5205 a         3452 c         2887 g         5224 t
ORIGIN      
        1 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
       61 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      121 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      181 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      241 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      301 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      361 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      421 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      481 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      541 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      601 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      661 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      721 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
      781 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
      841 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
      901 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
      961 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1021 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1081 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1141 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1201 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1261 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1321 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1381 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1441 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1501 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1561 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1621 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1681 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1741 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     1801 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     1861 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     1921 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     1981 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2041 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2101 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2161 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2221 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2281 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2341 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2401 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2461 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2521 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2581 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2641 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2701 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     2761 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     2821 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     2881 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     2941 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3001 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3061 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3121 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3181 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3241 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3301 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3361 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3421 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3481 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3541 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3601 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3661 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3721 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     3781 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     3841 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     3901 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     3961 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4021 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4081 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4141 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4201 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4261 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4321 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4381 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4441 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4501 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4561 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4621 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4681 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4741 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     4801 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     4861 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     4921 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     4981 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5041 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5101 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5161 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5221 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5281 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5341 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5401 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5461 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5521 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5581 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5641 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5701 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     5761 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     5821 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     5881 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     5941 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6001 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6061 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6121 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6181 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6241 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6301 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6361 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6421 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6481 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6541 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6601 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6661 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6721 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     6781 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     6841 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     6901 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     6961 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7021 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7081 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7141 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7201 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7261 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7321 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7381 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7441 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7501 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7561 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7621 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7681 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7741 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     7801 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     7861 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     7921 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     7981 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8041 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8101 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8161 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8221 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8281 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8341 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8401 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8461 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8521 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8581 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8641 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8701 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     8761 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     8821 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     8881 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     8941 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9001 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9061 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9121 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9181 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9241 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9301 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9361 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9421 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9481 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9541 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9601 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9661 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9721 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
     9781 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
     9841 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
     9901 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
     9961 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10021 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10081 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10141 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10201 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10261 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10321 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10381 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10441 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10501 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10561 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10621 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10681 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10741 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    10801 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    10861 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    10921 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    10981 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11041 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11101 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11161 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11221 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11281 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11341 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11401 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11461 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11521 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11581 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11641 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11701 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    11761 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    11821 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    11881 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    11941 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12001 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12061 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12121 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12181 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12241 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12301 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12361 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12421 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12481 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12541 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12601 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12661 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12721 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    12781 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    12841 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    12901 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    12961 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13021 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13081 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13141 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13201 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13261 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13321 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13381 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13441 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13501 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13561 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13621 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13681 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13741 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    13801 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    13861 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    13921 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    13981 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14041 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14101 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14161 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14221 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14281 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14341 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14401 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14461 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14521 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14581 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14641 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14701 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    14761 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    14821 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    14881 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    14941 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15001 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15061 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15121 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15181 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15241 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15301 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15361 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15421 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15481 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15541 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15601 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15661 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15721 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    15781 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    15841 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    15901 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    15961 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16021 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16081 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16141 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16201 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16261 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16321 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16381 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16441 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16501 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16561 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16621 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16681 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16741 tccaataaag tacataaaca taaataca
//
LOCUS       DAYYAO010000058        16724 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000058 DAYYAO010000000
VERSION     DAYYAO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.061"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      188..354
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            502..1059
                     /locus_tag="ACHARI_001873"
                     /pseudo
     CDS             502..1059
                     /locus_tag="ACHARI_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1117..3156
                     /locus_tag="ACHARI_001874"
     CDS             1117..3156
                     /locus_tag="ACHARI_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8442915.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3265..4416)
                     /locus_tag="ACHARI_001875"
     CDS             complement(3265..4416)
                     /locus_tag="ACHARI_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8442916.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4570..5100)
                     /locus_tag="ACHARI_001876"
     CDS             complement(4570..5100)
                     /locus_tag="ACHARI_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8442917.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5199..5549)
                     /locus_tag="ACHARI_001877"
     CDS             complement(5199..5549)
                     /locus_tag="ACHARI_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8442918.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5706..7235)
                     /gene="ahpF"
                     /locus_tag="ACHARI_001878"
     CDS             complement(5706..7235)
                     /gene="ahpF"
                     /locus_tag="ACHARI_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8442919.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7245..7808)
                     /gene="ahpC"
                     /locus_tag="ACHARI_001879"
     CDS             complement(7245..7808)
                     /gene="ahpC"
                     /locus_tag="ACHARI_001879"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8442920.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7952..8614)
                     /locus_tag="ACHARI_001880"
     CDS             complement(7952..8614)
                     /locus_tag="ACHARI_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8442921.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8798..10444
                     /locus_tag="ACHARI_001881"
     CDS             8798..10444
                     /locus_tag="ACHARI_001881"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8442922.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10435..11517
                     /gene="uxuA"
                     /locus_tag="ACHARI_001882"
     CDS             10435..11517
                     /gene="uxuA"
                     /locus_tag="ACHARI_001882"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8442923.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11625..13178)
                     /locus_tag="ACHARI_001883"
                     /pseudo
     CDS             complement(11625..13178)
                     /locus_tag="ACHARI_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13326..14467
                     /locus_tag="ACHARI_001884"
                     /pseudo
     CDS             13326..14467
                     /locus_tag="ACHARI_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14593..15672)
                     /locus_tag="ACHARI_001885"
     CDS             complement(14593..15672)
                     /locus_tag="ACHARI_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8442924.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15775..16215)
                     /locus_tag="ACHARI_001886"
     CDS             complement(15775..16215)
                     /locus_tag="ACHARI_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8442925.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16272..16682)
                     /locus_tag="ACHARI_001887"
     CDS             complement(16272..16682)
                     /locus_tag="ACHARI_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8442926.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         5722 a         3077 c         3053 g         4872 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      541 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      601 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      661 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      721 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      781 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      841 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      901 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      961 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
     1021 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     1081 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     1141 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1201 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1261 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1321 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga taaataccag
     1381 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1441 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1501 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1561 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1621 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1681 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1741 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1801 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1861 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1921 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1981 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     2041 aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga cggtgtagag
     2101 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     2161 gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc aactgttgcg
     2221 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2281 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2341 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aatgattaca
     2401 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2461 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2521 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2581 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2641 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2701 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2761 atccagcaag cagcaatgta ttctgttttt atcaacaatg gcacacttgt ctatcctaaa
     2821 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2881 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2941 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     3001 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     3061 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     3121 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3181 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3241 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3301 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3361 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3421 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3481 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3541 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3601 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3661 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3721 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3781 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3841 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3901 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3961 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     4021 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     4081 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     4141 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4201 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
     4261 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4321 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4381 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4441 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4501 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4561 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4621 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4681 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4741 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4801 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4861 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4921 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4981 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     5041 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     5101 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     5161 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5221 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5281 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5341 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5401 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5461 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5521 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     5581 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag
     5641 gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt tacaatcttc
     5701 atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc tcctgaaccc
     5761 attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc gaaaacgccg
     5821 gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt catttcgatc
     5881 gttctatcta gccaatccgt attagggagt aagccgacta gaatgaatac tcctgaaaca
     5941 gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc tacctgattc
     6001 gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc aagagagact
     6061 agtttatcct gtaagatttg atctgctttt aattctggta agaattctag aacatataca
     6121 tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc accaccgata
     6181 acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata agcaattcct
     6241 ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc acctgtcgcg
     6301 atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt aataaaatgt
     6361 tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg gtaactacgc
     6421 acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt accaatcata
     6481 ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac aagtcctgta
     6541 cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc accgccaatc
     6601 actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg gccagtgatt
     6661 ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc atttaaaaac
     6721 acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat cccaccttca
     6781 atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc ctgtacaacg
     6841 tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt ttgtgttatt
     6901 tttttgattt taagactcaa agattcactg attttcggtg cccgtccact aatttgtaac
     6961 atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa aacgattcca
     7021 ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc tcttttttcc
     7081 caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag tttttgacta
     7141 ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag tgctaaatat
     7201 tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat ctttccaaca
     7261 agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg acaaacttca
     7321 cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc actagcgtca
     7381 cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg atcaataata
     7441 aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat acgagaaatc
     7501 tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc tgatgtatca
     7561 tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc aacacctaaa
     7621 gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca aacaaaagta
     7681 aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt ttcactgact
     7741 tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt tttcccaatc
     7801 aatgacatag taaaacctcc aataataatt atttatttat aattattgta atttagttgg
     7861 agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa cgaaggttaa
     7921 ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg ggataacgtt
     7981 gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt tgaaatcgag
     8041 cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt tccatgatcg
     8101 tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct aaatgggtat
     8161 tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa caagcttcat
     8221 gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact gctttttctt
     8281 gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg gaacaacatt
     8341 caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct gcagaaacaa
     8401 gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt tcttgctgac
     8461 gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt tcctccagtg
     8521 ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt ttacgaatcg
     8581 cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa tcatttctat
     8641 gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt aactagtaaa
     8701 tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa aattcacttg
     8761 aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa acaactggag
     8821 gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg accgtgcatc
     8881 attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg gaaatcttta
     8941 tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat tgtccactgg
     9001 tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc aagattttga
     9061 tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac gtatattagc
     9121 tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg ataagatgat
     9181 tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg aaaaaggtta
     9241 tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata taaaaaatgg
     9301 tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata aacgtttttt
     9361 atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa acggtagtca
     9421 attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt atgtacaaaa
     9481 tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga cgatgattga
     9541 tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag agggcttttc
     9601 aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg tcaatacaga
     9661 ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga aattcgaaaa
     9721 agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga tgaaggtgac
     9781 gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac tggggtatag
     9841 gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg aaatcgggta
     9901 caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga tctttctgga
     9961 agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc agcgaatcgc
    10021 cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa aatatatgga
    10081 aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg ctgcttggct
    10141 tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc cagacccact
    10201 attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag atacatcaca
    10261 tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg tagatcttta
    10321 ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag caggaccaaa
    10381 tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt tatcatggaa
    10441 atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga aatcagacag
    10501 attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg agaggtatgg
    10561 cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt aacactaaaa
    10621 gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc ccgtgagcag
    10681 tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat agaagtcatc
    10741 tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta ccgcttagaa
    10801 gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc aagagaaatc
    10861 gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga acctgagcgc
    10921 ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga aaaacttcga
    10981 gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt aggggtcaaa
    11041 atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg aatcgtaaag
    11101 aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa cggaatcact
    11161 ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat tttagcagaa
    11221 ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt tttagctaat
    11281 cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat gtaccgcata
    11341 ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca tggaagaatg
    11401 atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt aggtgctgtt
    11461 tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga taattagtga
    11521 aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga tacataaatc
    11581 cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga agtaacttga
    11641 ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat atattctgag
    11701 atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa aatatagatt
    11761 tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat gttgcagttc
    11821 tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc tatcttaacg
    11881 atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac aattcgcggc
    11941 tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg cacattttcc
    12001 ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat ataaaattgt
    12061 tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat gaaagtagga
    12121 aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt tcgtgcgaat
    12181 gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac agtacaaagg
    12241 gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt taaaagtttg
    12301 tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg agctaaaata
    12361 ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat atctacagag
    12421 agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac gtctttcaca
    12481 gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt atctatcata
    12541 ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat aaaagccact
    12601 acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt ccaataaata
    12661 tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct gccattatta
    12721 gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt cattttcgta
    12781 tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga atttgtctga
    12841 cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta tcttccaaat
    12901 agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa aaaaggtttt
    12961 tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc taattctttg
    13021 atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata ttgtgtagta
    13081 ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt gctttttttc
    13141 ttcaagataa acgttttaat cgcttcttga gtttgcatga aagaggctcc tttttactta
    13201 aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa aaaacagtaa
    13261 acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca aggagtgaga
    13321 gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc ttatcgctta
    13381 ctataaatca gacagtccac acagaggaga cagaaacagc caaatggaca gaaaccaagt
    13441 gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat tcaggagtcg
    13501 caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat tcgggagact
    13561 tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccact ttgtcgattg
    13621 gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc ttcaccgtta
    13681 cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca gatggactac
    13741 cgaacgatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa gattcagcca
    13801 tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat gaaaataagc
    13861 cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata gaacctggag
    13921 aagaacccga tacagaaact caaccagagc cagacaataa atcaaaacct gaaataaccc
    13981 ctggagaaaa accagatgta actccagaac cagatacgga ttctggaaac caaacagtac
    14041 cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca gaggtagatc
    14101 cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac caaggaatac
    14161 cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt aaaaaatcaa
    14221 atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga atatttgata
    14281 gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct gtcctgaaac
    14341 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
    14401 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
    14461 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
    14521 aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
    14581 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
    14641 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
    14701 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
    14761 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
    14821 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
    14881 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
    14941 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtatt
    15001 taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
    15061 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
    15121 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
    15181 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
    15241 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
    15301 atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca tgtcaaatgt
    15361 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
    15421 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
    15481 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
    15541 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
    15601 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
    15661 cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaaga aaaaccgcta
    15721 aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc agtttcagcc
    15781 ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt gcagtgtatc
    15841 caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac cttctgcttg
    15901 tttttgcaac gcagcacctt gagcagtcag cgtaacattt acgattcttt catcacccgt
    15961 ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg gagtcagcgt
    16021 accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt tcttttccca
    16081 taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga ggaggggagt
    16141 gtagtgtcta atgatttctt tagaggcggc atataaagga aaacatactt gattaactaa
    16201 atatagatct tccatttttc cacctccgct gttttactat agtgtatcat agcggattta
    16261 cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc agctgtagct
    16321 tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc ttccagagaa
    16381 agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc actttctgaa
    16441 taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg ctgtttgatc
    16501 aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg ttcagggttg
    16561 gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt acgatcggga
    16621 ttatgcactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata catttttttc
    16681 ataaaattac cacctttaat tatattttat acaattgaat ttta
//
LOCUS       DAYYAO010000059        16480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000059 DAYYAO010000000
VERSION     DAYYAO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.062"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..307
                     /locus_tag="ACHARI_001888"
     CDS             167..307
                     /locus_tag="ACHARI_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8442927.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            377..796
                     /locus_tag="ACHARI_001889"
     CDS             377..796
                     /locus_tag="ACHARI_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8442928.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            793..1803
                     /locus_tag="ACHARI_001890"
     CDS             793..1803
                     /locus_tag="ACHARI_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8442929.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            1993..2066
                     /locus_tag="ACHARI_001891"
     tRNA            1993..2066
                     /locus_tag="ACHARI_001891"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2027..2029,aa:Ile,seq:cat)
     gene            complement(2580..2885)
                     /locus_tag="ACHARI_001892"
     CDS             complement(2580..2885)
                     /locus_tag="ACHARI_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8442930.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(3412..3484)
                     /locus_tag="ACHARI_001893"
     tRNA            complement(3412..3484)
                     /locus_tag="ACHARI_001893"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3449..3451),aa:Lys,seq:ctt)
     gene            3865..4434
                     /locus_tag="ACHARI_001894"
     CDS             3865..4434
                     /locus_tag="ACHARI_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8442931.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            4421..5281
                     /locus_tag="ACHARI_001895"
     CDS             4421..5281
                     /locus_tag="ACHARI_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8442932.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            5471..6175
                     /gene="yycF"
                     /locus_tag="ACHARI_001896"
     CDS             5471..6175
                     /gene="yycF"
                     /locus_tag="ACHARI_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8442933.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            6180..8015
                     /gene="walK"
                     /locus_tag="ACHARI_001897"
     CDS             6180..8015
                     /gene="walK"
                     /locus_tag="ACHARI_001897"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8442934.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            8012..9328
                     /gene="yycH"
                     /locus_tag="ACHARI_001898"
     CDS             8012..9328
                     /gene="yycH"
                     /locus_tag="ACHARI_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8442935.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            9329..10198
                     /locus_tag="ACHARI_001899"
     CDS             9329..10198
                     /locus_tag="ACHARI_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8442936.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            10253..11062
                     /locus_tag="ACHARI_001900"
     CDS             10253..11062
                     /locus_tag="ACHARI_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8442937.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(11165..11794)
                     /locus_tag="ACHARI_001901"
     CDS             complement(11165..11794)
                     /locus_tag="ACHARI_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8442938.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            11964..13256
                     /locus_tag="ACHARI_001902"
     CDS             11964..13256
                     /locus_tag="ACHARI_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8442939.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(13332..13994)
                     /locus_tag="ACHARI_001903"
     CDS             complement(13332..13994)
                     /locus_tag="ACHARI_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8442940.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            14219..14503
                     /gene="groES"
                     /locus_tag="ACHARI_001904"
     CDS             14219..14503
                     /gene="groES"
                     /locus_tag="ACHARI_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8442941.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            14554..16179
                     /gene="groL"
                     /locus_tag="ACHARI_001905"
     CDS             14554..16179
                     /gene="groL"
                     /locus_tag="ACHARI_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8442942.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         5548 a         2794 c         3463 g         4675 t
ORIGIN      
        1 agtggcacag cggttgacga tattgaagat gtcgatcagt tgaagcaatt aaatgctcta
       61 ctgacaaaac aattggcgat atcggtacag gaacaagaaa aaatgcaaca aatgttagct
      121 caattgacga tggaagtcgc aagtattaag aatggaggga aaagtaatga atagttttcc
      181 gggctttgaa aatataaaac aattttatga ttggggatgt tatacggacc aagatttact
      241 tgactatgta aacatgaact gtttaacaaa agaccaatat aagcagatta ctggaaatga
      301 aatttaatta agcacaaagt taaataaaaa gcgtactcaa acgagtgcgc ttttttgcat
      361 aaaggagaaa tagatattgg aaaaatattt aaatacattc tcagtaatga caggggtgat
      421 aggcgggaca attgttggtt tattgggagg gatggacaat atactacatg ttttaatttt
      481 tcttgtgggt gtggacttct taactggact tgctaaggca tggaaattga aagaaataag
      541 tagtgagata ggtttcgaag gattgttgaa gaaagtctta atcttcgttg tgattgccgt
      601 tgcggttgaa gtacagaaaa tcgttgggaa ttctatacct ctaagagaaa tagttattat
      661 gttctatgtt gcaaatgaag ggattagttt tttagaaaac atttcggtat tccttccttt
      721 gccagataag ttaaaagagg tattccaaca aatacgaaat gatactgaaa ataaagaccg
      781 aggaggaacc aaatgaaaaa gaaaatcttt gtaggagcta ttatagctct ttttttattg
      841 ccaataaacg cctttgctta cacgatcaac aatgaattca atttgggcgc aaacgaaggt
      901 agttctcaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
      961 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
     1021 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
     1081 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
     1141 aaagtaacgt ttgaaaagaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
     1201 aaatatggta ttccattaac attagaaacg ccttataacc aaccaggaat caaatcgcat
     1261 ttatgggtaa cacaaaatat ctggggcgat catacagatc cttacggtta tctttctgaa
     1321 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
     1381 ccgacaactt cagatgataa accagtcatt gatccaacta gagcaggtgc agcaaatcct
     1441 acactgacag atggaacaaa ttacgcccac attgatcagt ttggagaaat cgaaaatgcg
     1501 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
     1561 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccagat
     1621 gtaaaccaag cttataatac ttctggaaat gttggttatc atgtatcttt caatatgcgt
     1681 aattttccta gtaagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agagggaaac
     1741 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
     1801 taaaaatagc tcctcgttga ggagcagtac ataactatat tgacaactat aaaaattatt
     1861 cgataaaata gtgatgttat cgcatatctt cactatcacc cataaatagt cacactccaa
     1921 gctatgcgat aacaggtttg ttgccacaca ttctactggt tgattgttta tggctttatg
     1981 tggcaacaac cagtaccctt agctcagttg gtcagagcag acggctcata accgtccggt
     2041 cgtaggttcg agtcctacag ggtacattaa cgtagccatt tgaatcgttc tgtgttagaa
     2101 tttttttgaa gagtattata caagctaaag cttttcttca ttgccactca aatgagtggc
     2161 ttttttatgt atccttttat ggattaatga aaggatgttt cacatagtta tacttctgta
     2221 tatttgaaaa gttttacttt gatttttaaa tagaaagaca tttgggttaa attgtgagat
     2281 aataataaag aagagtttaa agcgcacccc aaaccacttc cccataagtg tgttacgctt
     2341 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     2401 aaaaaggctt acaattggag tggtagttaa ttagtgactt atttttgatt ttatagcact
     2461 gatactataa aatatagata tcatcatatt acacaatctt aatactaact taaaaaatat
     2521 ctcctttcac aagtatggtg ataaaattcg ttccgggcta cctttttagg tagcctactt
     2581 taatctttat acctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga
     2641 tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca aaaagtatac acccttcttc
     2701 attctagttt tcgcagcagg aattttgaaa tagttcttat tagaatagta gagattgatt
     2761 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
     2821 ttgacaaatt tttctgatgg atcgtattta aaccaaagca gatgttcctc tagctcgatt
     2881 gtcatgtttt tcacctcaac gaaagtatac gaactaatgt ttgttttgta aaggataaaa
     2941 atagggtagc aaccggtacc caaactagta cccatttatc aaaaacgacg aaatttatgt
     3001 aaatagaaaa ataagaataa acattgatgt atcaacattt atatatcttt agaacaaatg
     3061 aaaagggtgg ttctcataag aaaaacatgt tcgcacttct aggcaaacca ggatttgaag
     3121 accttgctaa agaattgaac gaacgcctgt aaaataagga tttgtcgcta gatttaaaaa
     3181 atcagtgata gaaacatgtt tgctttaaaa tgaaactagt gatagtttta gtgatagtga
     3241 acttaaaaaa agcactcaat ttgagtgctt ttttattgtt attaaaaatt aaaaaactaa
     3301 aatacatacc cgaatttcat acccgaaata taaaaaatgt catcacgtac atctttttcg
     3361 aaactaccga agtagccaaa gatgtactga tgacgtgggt ttggcaccca atgggccgtg
     3421 aggggctcga acccgcgacc cgctgattaa gagtcagctg ctctaccaac tgagctaacg
     3481 gcccgtacaa aaagtactta ataatagtag cactaagaat tttaaaatgc aagactacat
     3541 ttaaagaaaa tcagaaaaat ctttacttcc ttataggaaa tcattcatag attattcaca
     3601 tttgctcgct attatataag cataccaaca aacaaagttc ttgcaagaga acaaacccta
     3661 acactttttt cattttttac tcctttcccg ccgtctcccc ggcgggtctc ctgttcttaa
     3721 cgataaaaga caagaaggct tagccttctt gttttttgtt ttcatggaat cccgctaatc
     3781 aaatcaccta gttaaagcaa ctagattgaa tgtttatcat aagtctgata tcatgtttat
     3841 gaaatgaaaa aaggagcggt tatgatggaa aaaatgcgtc agcaattaat ggagtggggg
     3901 aaggaactag aaacattccg tctgcctcac tgggaagaac tccctgactt agaactttat
     3961 atggaccaag tcagaacgtt agttgatcgt tatctttcac ctgtgataca aggagaaaag
     4021 catccattgc ttacctcttc tatggtaaac aattacgtta aactaggttt gataccagca
     4081 ccagttaaaa aaagatataa caaagaacac gtagcatttt tacttgcaat cactacgttg
     4141 aaacaagtat tgactattcc tgaaatcaaa gaagggatat tgttccaagg aaaaacagta
     4201 ggaattcgtg aagcctataa tctattttgc gatgaacaag aagcagctgt gtggatggtc
     4261 agccaattag cacaaggtaa atctcatccg caaaaattcg accaggcaac ccctgtagaa
     4321 tatattgctg taagagcggc aactttatcg tttgcaatga aacttcttgc ggaaaaaacg
     4381 attgttctag aaaccgaata tttaaaagag gagaaaacaa atgaaaaaca ataaaatcgc
     4441 aattttagta gattcaggta cggatgtgcc agaagaattg ataaaaaaat acgagatgta
     4501 tatgatccca ttgaaaatta tttataaaga tcaggtatat acagataaaa ttgacattac
     4561 ctctgaagag atctatcaac gtttgccaga ggaaatccct ggtacatctt tgccggatgg
     4621 tgagtcgatc acagaaatct ttgagcaaat caaaaaagac ggttatgaaa aggtacttgc
     4681 ggtaacaatt tccagtggat tgagcgggac atataatgtg gttcgtttaa ttgcacaaca
     4741 gcaagaagaa ttggaagcat atgtactaga tacgaaaaat attggtattg gtgcaggttt
     4801 ttctgcgatc caagcagcaa aatggctgga agaaggagta gaatgggatc aactgatcag
     4861 taatctgaat gagttagtcg aaagaacaaa agttttcttt aatgttgcga cattggaata
     4921 tcttcaaaaa ggtggacgga tcggtttagt tgcttctatt gtaggaaccg ctttaaagct
     4981 taacccaatc atctcatgta atgaagaagg gatttattac acagtaggaa aagctagggg
     5041 acgtaaaaaa agtttggata aaacggttgc ttacgtcaaa gaaaaaatcg gtaatgcaaa
     5101 ggtcttcaac ttagctgttg cccatgggga tgcgaaagaa gaagcaattg aaatggaagc
     5161 gagacttaaa agagagttcc cacaagcaaa ccaaatctac tttggccaga tttctccggc
     5221 attagtagtc cacactggtc cgggattatt gggtgtcggt gtacaagttt tagaaggata
     5281 aatcaaaaaa aggaaacact gaggtgaaaa tcagtgtttt ccttttttat tgaagttaga
     5341 acaaaaaata ataaagtatt ttgcactttc attttaattt tctcttttgt tttataggca
     5401 aagatgaaac aaaagcagaa atacggtata atgagaaggt atgacaataa tatataagag
     5461 gtggctcttt atgaaaaaag ttttagttgt cgatgatgag aagccgattt cggatatcgt
     5521 caaattcaat ttagccaaag aaggctatga tgtttatact gcatatgatg gggaagaagc
     5581 cttggaaaaa gttgctgagg tagaacctga tttgatttta cttgatttga tgctacctaa
     5641 aatggacggc ttagaagttg ctcgtgaggt acgcaaaact tatgatatgc cgatcattat
     5701 ggtaacagcc aaagattctg aaattgataa agtcctaggc ttagagttag gcgccgatga
     5761 ttacgtgacc aagccatttt ccaaccgaga attagttgca cgtgtcaaag caaatcttcg
     5821 tcgcggtgcc acggctgcga aggaaccaga agaggcagca cctgcggaat tgacgattgg
     5881 tgatttgacg atccatccag aagcttacat ggtgacgaaa cgaggagaaa caatcgagtt
     5941 aacacatcgt gaattcgaac tactctttta cttggcgaag caccttggac aagtgatgac
     6001 aagagaacat ttattacaaa cggtttgggg ttatgattat tttggagatg tacgaacagt
     6061 ggatgtaact gtacgtcgtt tgcgtgaaaa gatcgaagat aatccaagcc atccaaatta
     6121 tttagtgaca cgtcgtggtg tcggttacta tttgcgtaac ccagaacagg agtaattgga
     6181 tgaaaagaaa agtccgcttt tttcgatcag tcaattttaa gatcgctatt acctttattc
     6241 tgattttatt gatctcgatc gaaattattg gtgcctactt tatacgtggt ttagaacgtt
     6301 ccacgatcaa tacatttatt aaagatatga atcaaacggt ggaatcatta gcaacaacaa
     6361 tcagcccgga attgaatcga aaagataatg cagatgatga agaggtaaat gcgaatatca
     6421 aaagatttat tgaaaacagt gcgactagcg atatcatcga aatacgggtc gttgacgaaa
     6481 aagggatcat ccgaggaaca accgatgtca atgaacaaag cgctgtggga aagaaaaatg
     6541 attatgttga tatcaatgat ttcacgacta aacgttatgt tgctttagat aacgacgata
     6601 aacgggtgaa tatcaatgtt caaccgatcc tgtccccgac cggcgatgcc gttatcgggg
     6661 ctctttacgt caaaagtaat atcgaacaaa aatattcgga aatcaataac acagcagtca
     6721 ttttctttac tgcctcacta attgcggcat ttatttcaat gatcgtatct gttttagttg
     6781 ctcgttcgat tacacagccg atcggtgaaa tgagagaaca agccattcga atcgctagag
     6841 gagactacag tcggaaagtc aaagtgcatg gacaagatga acttggacag ctggctgata
     6901 ccttcaatca gttagctgaa cgtattgaag aagctcaaga tacaatggaa gctgaacgca
     6961 atcgtttgga tagcgtgcta tcccatatga cagacggcgt cattgcgaca gatcgcaggg
     7021 gtaaagtcat tacgatcaat gatatggctg tttccttgct agatatcaaa aacgaggaag
     7081 caatcggaca atcgattttg acacttttag atatcgaaga agaatatacg ttgcggaaat
     7141 tattagaaga tccaaacgaa atggtacttg accgttcaac cagtacattg gaaagtgatc
     7201 agatgatctt gcgagtagac ttttctatga tccgtcgcga atcaggtttt atcagcggat
     7261 tagtagctgt cttgcatgat gtgacagaac aagaaaaaac agaacgggaa cgtcgtgaat
     7321 tcgtctctaa tgtttcccat gaacttcgta caccgttgac aagtatgcgt agttatatcg
     7381 aagcattgag cgaaggtgcg tggaaagacg aggaagtagc accaaatttt ttgaaggtaa
     7441 cgctagatga aacagaccga atgattcgaa tgatcaatga cctcttaaat ctatctcgca
     7501 tggatacagg taatacccag cttcaattag aatatgtcaa tttcaacgag ttagtgaatt
     7561 ttgttcttga ccggtttgat atgatggtgg gaaatcagga aaaaaattat aagatccgtc
     7621 gtgaatttac gcaaagagat ctttgggtcg aagtagatac agataaaatc atccaagtag
     7681 tcgataatat catgaataac gcgatcaagt attcaccaga tgggggaacg attacttgtc
     7741 gcttattaga aacgcataat aacgtggttt taagtatcac cgatcaagga ttggggattc
     7801 ctaaaaaaga tctgaaccga gtgttcgagc ggttctatcg agtagataaa gcacgtgcaa
     7861 gagcacaagg tgggacagga cttggattag ctatttctag agaagtgatt aaggcgcatc
     7921 gaggcgctat ttgggcagag agcaaggaag gaaaagggtc gactttttat atctcgctgc
     7981 catatgaacc ttatgaggag gaatggtggg aatgagaatc tctgaaaaag tcgttcgtat
     8041 aggactcatt ttgatggttg cgttaagcat atacttctct tatgcaatct ggctgagccc
     8101 agcaggaaaa agttcaatca acgtagatga gacaaacact caaatgatcg agtctcaaag
     8161 caatcgaaaa acttccgaag tctttcttcc gcttcatgtt acatggcacc attcgggaat
     8221 gatccaagaa accaacagtg aaaatctcgt gtaccgattg caattgatca tcgataaagc
     8281 aagatttgga aagctttctg aagtagtttc aggagacaaa tcacagtttg aacaggtgaa
     8341 aaatctgaac tctggttttg agttggccta taatgcgcct tttttgctaa gagaatacaa
     8401 ggaagcattt gatttagatt tagatgtgtc gaacttggca aaagaaagcg aaaatatgta
     8461 ttttactaga atacaattcg atcaaggaaa taaaaaaatc cgatttttga attattcgaa
     8521 ccatctcgta tatgaagcaa atatttcgtt gaattggaca gatatcaata aagaattaac
     8581 agcaaaagac acaaaatgga cagaaatgac aacagaacca tcgacgatcg acacgcaata
     8641 tgctacgaaa cattcgatca aactgaaaaa gtatagttat atcctgtcac agcaacccta
     8701 tacacttttt agaaatgctt ttttccagaa accagatagc gtgaaaaata atgatgaaag
     8761 caatgaattg attttttacg gtgggaatga aacaatgtct atccattcgg aaacgcaagt
     8821 agttgatttt cgtggagagc tgccagagaa aaaagaaaat gctagtgtat tttctgaaag
     8881 tttcccctat attagcaaat taggcagttc tttagggaac ctcagatatt ttgaccgcag
     8941 tgacaatacg atcgattatc ggatattcgt ggaaggcttc ccggtttttg ggacagacag
     9001 cagaggacag attggtgtgg aagttggcgg tgaaacgaat ggttctgtgc aagtgaatat
     9061 ccaaaccagt ttgaatacga ttcaagtgcc gataccttca gacgaagaag tagaacttgc
     9121 aagtacgaat gaaatcatcc agcagttaaa agctaaggga gcagatagca acaagattgg
     9181 ttcagtgatc gttggctata catggcaaaa tgtcaaagaa acgaatcgtg tcgttgatct
     9241 cttacctgaa tggtatgtga aatataaaga taactggtat tcagctaacg accttctgga
     9301 aaaattacct ggattggagg cgaattaatt ggatttcaaa aaaatcgaat ggatattttt
     9361 cgtcgctttt ttagggttga atgtgttttt attcagcagt tatcatgacg cggtatacca
     9421 agaaaacaat gtcattcgct ctaatcagac tgaatcaatc caacaacgtc tggctagcga
     9481 cgatatcact tacacgggga agatttcgtc tgagaacaag caaggctatt atcttagcgc
     9541 ggagcagacc aatcttagca cttcattagc gaattatcgc aaggctggta aaagcggctt
     9601 gctggcaagt ggtgtgtttt tagacggtga tgttttgacg aaatctttat ccagttcaga
     9661 tgctgaaaag aacgtgatcg atccagataa aattagtagt tccttggaag attttttatc
     9721 taacaaagac aatattttat tcggtaatga ttatgtgtac acgaaaaact tttctcattt
     9781 agaagaagaa ccactagaaa tccaagcaag ccaagagtat gaaggcatcc cattcaatga
     9841 tgatacagca aaaatcgttc tttctttaga aaaaaacgat aatgattata gcatcacgaa
     9901 atacatgcag acgcatttat ctgatatcga gcagctgcgc gaaaagacgg aactgtatac
     9961 ggaagaggaa gcaatcaata cattgtacat caataacaaa ataccaagag gatcaaaaat
    10021 tttatggcgt cagctagctt attcctgtat tctgaaagtc cgggaaaaaa atgtgtatgt
    10081 tcctgtttgg catgtagcga tcgaaacaag cgataaagtg gtccaaattg agagtgtcaa
    10141 cgctttcagc aatacgatcg tgacaaacaa tactgtacca aaagtggaag atcaataaaa
    10201 aagcgagtat aatgaaaaaa gtttacaaag taagaaggga agtaggagtc aaatggctga
    10261 agaaaaacca gcttttaaaa tcagtatcct agcaagtgga agttcgggta attcacttta
    10321 tatcgaaagt gaaaaaaaac ggcttttggt cgatgcagga cttagcggta aaaaaatcac
    10381 ttccttgatg gctcaaattg atcggacacc ggaccaatta gatggaatac ttgtgacgca
    10441 tgaacatcga gatcatatac atggcgtggg tgtcttggct cgtaagtatc atttggatgt
    10501 ttttgccaat cagcagactt gggaagcgat ggaaccactg attggaaatg taccagtgga
    10561 acaaaaacat ctatttgaga tggggaaagt cagaacattc ggcgatatcg atattgagag
    10621 tttcggcgtc tcccatgacg cagcggctcc gcaattttat cgtttccata aagaaaatcg
    10681 gtcattcgtc atgttgacgg acaccggata ttgcagtgat tatatgcgag gtgtcattga
    10741 aaatgccgat ggttatttga tggaaagtaa tcatgatctt gagatgttac gcatgggacc
    10801 ttatccgtgg aatttaaaac agcgtatttt aggagataga ggacatcttt ccaacgaaga
    10861 cggtgcactt gtcatgactg atgtgatcgg taatcggacc aaacgaatct atcttggcca
    10921 tcttagcaag gaaaacaata tgaaagaact tgctcattta acaatggaaa atgttttgaa
    10981 agaaaaagat tttggcgttg gacatgattt tgaaatctat gatacagatc cagatgaagc
    11041 aactgagcta ttttcgattt aaacagttca atcaataaaa atataaaaag cagaagataa
    11101 aatcaatcag ggttggtttt atcttctatt tttatttttt tagcatatta aaaaaataac
    11161 cgtattatcg actgactaca ctgatgtgct gcttttcatg tttctcatcc agtatctcat
    11221 ctactaatgc aatcgcgtaa tctgccatgc tgatcgtact gtcgccagca gcgtttcttt
    11281 gtaaataatc atcacttaac aaataagtac ctgtacgttt gccatgtgga tcaaataaag
    11341 aagctggact gatataagtc caatgtagat ttttagattg tttcaatttt aacaatcctt
    11401 cgtacatatt gaaagctgtt ggatagaagg gtgcattttt aggtacttgg tcaatcatac
    11461 gagtcgtctt ttctgtatct aagaataacg aagaggcacc cccaacgaca attaagcgtg
    11521 tatcggttcc ttcaagcatc tcaatcaaat gggacaaaga agtttgatgc atttcttctt
    11581 ttcctttggg agcgttgaaa gcatcaacga caacatcaaa agatgcaaga tcagtagttg
    11641 tcaactcaaa taaatccttt tgtataaacg gaacatctga tggcaatttt tctggatgac
    11701 gcacgattgc cgtcacgtct aaatctctgg acaatgcttc gtctaaaata aatgaacctg
    11761 catggccagt agctccaatg agtgcaattt tcataaaaaa cctccttttt tattcctgtc
    11821 cattgtaagc agttgctgaa tattcgtcaa agaatccgat ttttgaaaag ctcagtgcgc
    11881 attcctttca tggttcgtga tcattgggta tagttaaatt aagaaaactg attgataaaa
    11941 atagaagttg aaggagataa gaaatgaaag agtggttaga tcgtgccttt ttaccaatga
    12001 taacatctgt tgtgaatctt agacccttaa ctgctttgcg taatggaatg acttacacga
    12061 tcccaataat tattgttggc tcgctgtttt tcttatttgc tcattttcct gtggattaca
    12121 tcgctcagtg gatagacaaa acaggatttt ctatgtattt tacacaagta taccaggcta
    12181 catttgatgt gatagccttt tggatcgttt ttgccattgc atacgcttat gcaaaagaag
    12241 aagacatcga tcgcttacca gtcggcataa cagccttgtc tgcttatcta ctgcttattc
    12301 agccactaaa tactcagata gatactgtct ggacaggtgc tgaagggata ctaggagcag
    12361 ttacaactgg gctcatcatc ggatggggct atgcacgttt agtaagactt gcgaaagggt
    12421 ggaagcaaag agagaagata cctgaaaatg tttggcagtc ttttacttca ttgattccaa
    12481 tcattgttat agtcactttt acgcttatcg gttcagcggt ttttcaacag ttgttcagaa
    12541 tgactgttgt tcaaggagta tggacatatg tccagcttcc cattcaatcg ttcatagaca
    12601 cgttgtttgg tgtgctttta ttaggttttt ttgttccgtt tttatggtta tttggtcttc
    12661 atggatctgc gatggtcaat gggttggtta gtcccgtctt gcaagcgaat tcattagcga
    12721 atgcagaaat cttagcctca ggacaagagt tgacggtggc aaatggcgga catatcgtta
    12781 ctcagcaatt tctcgatcag ttcatgacgg ttacgggtgc tggattgacg ttaggggctg
    12841 tattttttat gacggttttt gcgaaatcga agaaatacag agaattagga aatcttgctc
    12901 ttttaccagc ctttttcaac atcaatgaat caattatttt ttccacaccg atcgtgatga
    12961 acccgatgat ggctgttcct tttattttcg caccggtcgt ttcaggattg attacctatt
    13021 ccgctttata ttttggattc gttcctttat ttacagcagt ccaagtccct tggacaacac
    13081 cgccgatcct ctcgggtttc ttagtaggcg gagtgcctgc agcgatttta caaggtatcg
    13141 tgttgtcaat tagttttttc atttatttcc catttttgaa aaaaatagat tctgcaaact
    13201 gcaaaaagga aagacagaca aaaaatgatg gagcagcaga aaagatgaac gactaaaaaa
    13261 caaaaatact cctgtcgctt tagattggga aaatcaccaa tctaggatga caggagtatt
    13321 tatagtcatg tttatactag ccctttttgg acagctaatt gaacaagaac aactaatgtg
    13381 ttcattccta cgtgtgtgat catcgatgtc cagatccgac cagttgcttt gtactctagg
    13441 ctgaagaaga aacctaggaa aaagtagatt agcagatgac catcattatg agcaaacgca
    13501 aacagcaaag agctgatcac tgctccgacc caaaaatttg aatatcgact gatgatcccc
    13561 agtatactgc ggcggaatac gaactcttcc atgatgggtc cagcaacagt tgtcgccaaa
    13621 ataaaagcgg gtgcttccat gatcatctgg atgatatttt gtgtattctc ggaaggggtt
    13681 gctttgccaa acaatacaga ttctaacatg acagcgatgc tttgcagaat caatgcgaca
    13741 aagattccaa ccaatccata aataacaatc tttggagcag aagctttttt taccgctgat
    13801 tcgatcgcta agggttcacg ttgcttaaaa taaaaaatag ctaggacaac ggcgcctagt
    13861 atatacatga ctgcagttgc tgtgatcaat tcagacgtag attttacaag tcctatcgaa
    13921 gctaggatca agggtgagat aaaaattaga ccatagcaaa aaatgctgag gaagctatat
    13981 ttttttaaag acatgtaatt cctcctttta agggataaca agcatgcaag gcaagcttat
    14041 atcgattgat ttcaattata tcataatttc aataagcagc tgtcagaata aaatttcttt
    14101 tctttgattt aaaagcttgc aaaaaagagg gagaatgagt attattaaaa atgtaggtta
    14161 gcactcgaag gttaagagtg ctaacagatt aaaaaaagag ttatcggagg gatttatcgt
    14221 gttaaaacca ttaggtgatc gcgtcattat cgaagtcgca aaagaagaag aaaaaactgt
    14281 cggaggaatc gttttagctt ctgctgctaa agagaaaccc caaactggga cagttgttgc
    14341 agtcggtgaa ggccgtttgt tagaaaacgg tgaaaaagtt cctgccgctg taaaagcagg
    14401 cgaccaagtt atgttcgaaa aatacgccgg tacagaagta aaatacgaag gaaaagaata
    14461 tctgatcgtc gcaggaaaag atatcatggc gattgttgag taaatttgga agataaaaaa
    14521 ataatcaaaa ataatcaatg aggtgaatca atcatggcaa aagaattgaa atttgcagaa
    14581 gacgcacgcg cagctatgtt acgcggggta gataaattag cagatacagt gaaagtaaca
    14641 ttaggaccta aaggccgaaa tgtcgtacta gaaaaatctt acggctctcc attgatcaca
    14701 aatgatggtg tgacgatcgc aaaagaaatc gaattagaag atcattttga aaacatgggt
    14761 gcaaaacttg tatcagaagt agcatcaaaa acaaatgata ttgcaggaga cggtacaacg
    14821 acagcaactg ttctgacaca agcaatcgtt cgcgaaggtt taaaaaacgt aactgcaggt
    14881 gctaatcctc taggcattcg tcgcgggatc gaattggcaa caaaagcagc agtcgaagaa
    14941 ttgcataata tctcaactgt tgttgattca aaagaagcca ttgcacaagt agcagctgtt
    15001 tcttcgggtt ctgataaagt tggacatttg attgctgatg caatggaaaa agttggaaac
    15061 gacggtgtca tcacaatcga agaatcaaaa gggatcgaaa cagaactaga tgttgtagaa
    15121 ggtatgcagt ttgatcgtgg ttacttatca caatatatgg taacagataa cgacaaaatg
    15181 gaagctgttc tagaaaaccc atatatcttg atcacagaca aaaaaatctc taacatccaa
    15241 gatattctgc cattgttaga acaaatcttg caacaatcac gtccattgtt gattattgct
    15301 gacgatgtag atggtgaagc attgccaact cttgtattga acaaaatccg tggaacattc
    15361 aacgtagtag ctgtaaaagc tcctggtttc ggcgatcgtc gtaaagcaat gcttgaagat
    15421 atcgctattt tgacaggtgg tacagtaatt actgacgatc taggtttaga attgaaagat
    15481 gtaacaatcg aaaacttagg aaatgcttct aaagttgttg tagataaaga caatacaacg
    15541 atcgtagaag gttctggaga aaaagaagca atcgaagctc gtgtgcaatt gatcaaaaac
    15601 caaattgctg aaacaacttc tgattttgac cgcgaaaaat tacaagaacg cttagctaaa
    15661 ttagcaggtg gtgtagctgt tgttaaagtt ggtgcagcta ctgaaacaga attgaaagaa
    15721 ttaaaattac gcattgaaga tgcattgaat gctacacgtg ccgcagtaga agaaggaatg
    15781 gtctcaggtg gtggtacagc tctagtgaac gttatcagca aagtttctgc tgtagaagca
    15841 gaaggagacg tggcaacagg tatcaagatc gttgttcgtg ctttagaaga accaattcgt
    15901 caaatcgctg aaaacgctgg ttatgaagga tcagtgattg ttgataaatt gaagaacgtg
    15961 gaactaggca ctggtttcaa tgctgctact ggagaatggg taaacatggt tgaagcaggt
    16021 atcgtagacc caactaaagt aacacgttct gccttgcaaa atgccgcttc cgtttctgcc
    16081 ttgttattaa cgacagaagc agtcgttgca gataaaccag aaccagcagc gccagcagct
    16141 cctgcaatgg atccatcaat gatgggcggt atgatgtaag aaaaagcaac ttgcttatct
    16201 acaactgatc attagtgaaa gacagataaa aagaggctgg aacagaagcg tttaactcca
    16261 agaaataaga agaaattcac ggaaattgct tttcaaattt ttgtgaattt cagcttattt
    16321 ccgaaggagt tgcttctgct tccgctgttt atacgtgttt agagcgtgag acaaaagtgt
    16381 tctttacttt tgtcccacgc tcttttttgt gtgtaattat aatgaaaata gttattttta
    16441 atagttataa tattttatct attaaaagta aatatccatt
//
LOCUS       DAYYAO010000060        15921 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000060 DAYYAO010000000
VERSION     DAYYAO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.063"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            224..892
                     /locus_tag="ACHARI_001906"
     CDS             224..892
                     /locus_tag="ACHARI_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8442943.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1092..1412)
                     /locus_tag="ACHARI_001907"
     CDS             complement(1092..1412)
                     /locus_tag="ACHARI_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442944.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1449..2831)
                     /locus_tag="ACHARI_001908"
     CDS             complement(1449..2831)
                     /locus_tag="ACHARI_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8442945.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2987..4174)
                     /locus_tag="ACHARI_001909"
     CDS             complement(2987..4174)
                     /locus_tag="ACHARI_001909"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8442946.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4181..5212)
                     /locus_tag="ACHARI_001910"
     CDS             complement(4181..5212)
                     /locus_tag="ACHARI_001910"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8442947.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5258..6682)
                     /locus_tag="ACHARI_001911"
     CDS             complement(5258..6682)
                     /locus_tag="ACHARI_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8442948.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6857..7762
                     /locus_tag="ACHARI_001912"
     CDS             6857..7762
                     /locus_tag="ACHARI_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8442949.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7853..8530
                     /locus_tag="ACHARI_001913"
     CDS             7853..8530
                     /locus_tag="ACHARI_001913"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8442950.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8527..8697
                     /locus_tag="ACHARI_001914"
     CDS             8527..8697
                     /locus_tag="ACHARI_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442951.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8811..9707
                     /locus_tag="ACHARI_001915"
     CDS             8811..9707
                     /locus_tag="ACHARI_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8442952.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9762..11132)
                     /locus_tag="ACHARI_001916"
     CDS             complement(9762..11132)
                     /locus_tag="ACHARI_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8442953.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11370..12116
                     /locus_tag="ACHARI_001917"
     CDS             11370..12116
                     /locus_tag="ACHARI_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8442954.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12113..12952
                     /locus_tag="ACHARI_001918"
     CDS             12113..12952
                     /locus_tag="ACHARI_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8442955.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12953..13902
                     /locus_tag="ACHARI_001919"
                     /pseudo
     CDS             12953..13902
                     /locus_tag="ACHARI_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14043..14795
                     /locus_tag="ACHARI_001920"
     CDS             14043..14795
                     /locus_tag="ACHARI_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442956.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14814..>15921
                     /gene="queG"
                     /locus_tag="ACHARI_001921"
     CDS             14814..>15921
                     /gene="queG"
                     /locus_tag="ACHARI_001921"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8442957.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVIN"
BASE COUNT         5159 a         2988 c         3210 g         4564 t
ORIGIN      
        1 tccaatttcg taaaacaacc gtaacggttg tgtaaaaata tcattttaga gcataaaaaa
       61 aaggagtgga tttctccact ccaaaaaagt ctaacttttg ggggtcacta cagtgtcaca
      121 gtcctttttt tagtaaaagg atacatttct tccttgtata ttccatgcgt gttcgtgaga
      181 aagcatatat aatttaatta acagatagtt agggggagaa aaaatgattt tttcaattac
      241 aatgaaactg gtgttcgggt tgatcggttt attattagtg gtacgtttgc ttgggaagaa
      301 atccatgtca gaaatcacac cgtttgattt agtctatacg cttgttttag gtgggatctt
      361 agaagaatca acctatgatg acaatgtcca tgtagggcat gtcctatttg ccattgcgct
      421 ttgggcagta atgatttatg gtatcgaacg tatcgtccaa aaaaatgaaa aggtcaatcg
      481 ttgggtcaaa ggcgagccat ctgttctaat caaagacggc gtcatcaaca tgacagagtt
      541 gacaaataac catatcgaga tggagcagtt gcgagcaatc ttaagacagc aagaatgctt
      601 ttttcttgaa aatgccaaac atgttatcct agagaatgcc ggtcaaatga gtgtattgaa
      661 aaaatcagat gaagacaaag ctttatcgat cctgcttgta gatgaagggc aaattcagca
      721 taaagtgctt cagtccaatc aattgactga agcatggctt atggagaact tgaagaaaga
      781 aggctatgca gatgtaaaac agctgattta tgttgaatgg tcagaagaaa aagggctcta
      841 catactaaat aaagaagacg tcatgcatga atcttaccga atagatggat agatagacag
      901 aggttgtgaa atcagcgcct tggcttgtta ctcaaaacta agcgactttt tcacaacctc
      961 tgatatctaa tatacgttgt tccagaagtt gcttcttcga aaaataagcc aaaattccac
     1021 aaaaatttaa gaagcaattt tcgaaaaatc ctttcttatt ttttgaagtt gatcacttct
     1081 gtcacaacct ctcatgtcac aaaccatttt tttgtttcct ttaactcaat ataccgtacg
     1141 tcttcccaca atatcggctg atggtcaatc ataaagtaat cttcataggc ttcaccaatg
     1201 aagtgccctt cgacctcatc caagagtctt ccaaattcat cttttaaatt gagctgaatg
     1261 attactgctt tacgagataa aaaggcttct gtcagtatac tatcgatttc ttgcaatgtc
     1321 atttgttgtt taggaagagg ctgtttgcgt gcttcttctt catttttttg ataccgttga
     1381 ctagttcttc catcgcaaaa gcagtccccc attttaatcc aaatggccga tctcgatact
     1441 cgttatagtc aatgaatttt tttgctgtcc gtctcatctt ttccaccttc taatccactc
     1501 ataccacctg catgcccgcc aacgagagaa ctacgtgcga tggcgcgtcc tccttgcaag
     1561 agactatgcg catggatgat tgattgaaag ccgtattttt ttctaatcgt atcgacaaca
     1621 aaatcaactt tcgcttcatt tatctgcttt tcaggatctt caaaaaggtt aagctgtaaa
     1681 cctgtcgcgt atacgaggtc ggaacagttc acgcctacac gccgaatatc ctggtagtgg
     1741 taatgctggt ggaaaatggt caataaagca tctgcaagat ctctgctagc atttgtcgct
     1801 tcgattttca tttgctggtg aaacccggtc ttgccatttg gatcaagata gcctaaagag
     1861 tatcctaccc ataagctgac tacttgtgtt tttgctgcag atcgtctcaa gcgagtgccg
     1921 acttggtcgg ccatttcttt gataacgatc tcgatttctt ctcgtcttgt ataatctcta
     1981 tctaggattt gactatttcc gatacttttt gattttggtg tatattcttg tcccaaaaaa
     2041 cttcgatcga tgccccaagc atgggcataa agctgagtac cgatgacccc tagcttgctt
     2101 ttcaagtgat aataatccgc gtgagctaaa tcataaacag aatgtatacc catttgtttc
     2161 agacgttttg ctgttcgttt gccgattccc caaaagtcag tgatatctga gattttccat
     2221 aacttttctg gtacatcttc ataacgccat tcagcaaaca tttgctttcc gtcttttgag
     2281 ccattgtcta aggctagttt agcaagtagc gggttatcgc caattcctat agtcgtgtag
     2341 attccggtct gctcaaacac atctttatgg atcagtttag ccatgtcgta cgcatttgtt
     2401 ttaccaaaga gtgccatgct atttgttaca tcaaggaagc tttcatcgac ggaatagaca
     2461 tgatggttcg cttcgtctgc atatttttta tagatagaat tgatttctct gttcttttcc
     2521 atataatagg ccatccttgg ggcaacaata tataaatcac ttggataggg gaagggaaga
     2581 tctctggcac ggctgatatt ggtgatgccg taagcttttt ttgctgcagg actggaagcc
     2641 aagattaatc cgctaccccg tgtttctgtc gaatcggacg gataggacat gactactaat
     2701 ttagttgtaa ggggatcaag atccttttca acgcactcta cggaggcgta aaaagatttg
     2761 cagtcaatac ataaaatatc tcttgaaggt tctttttcat aaacaaatac cggattttga
     2821 ttcgcccaca taacaatcac ctcttcaaca caattagaac acacgttctc tttccgtgtc
     2881 aataaaaaac agcaattttc aaatacgagc aaaaggctca catttggaaa ttgctgagag
     2941 gttattttta tctatttgtt tttagctgag atcgttgatt tttttatcaa acatgaacag
     3001 cttctggatc gatctttgtt gtcccatttc caatgtgtgc aacataaaat tccggagcat
     3061 agccgacaac tttcatgtat tcatcataga cattgttttt gaaggcaggt atattttctt
     3121 ctttgacaag agcaatcgca cagccaccaa atccagctcc tgtcattcgt gcacctaaga
     3181 caccttcttg tttttgagca gcatctacta gtgtatctaa ttcgatccca gttacttcat
     3241 aatcatagcg aagagagtgg tgagaatcat ttaacagttg accaaatgct tctaagttcc
     3301 cagcttctaa ttcagctttt gccttcaaag tccgctgatt ttcagaaacg gcatggcgag
     3361 ctctgcgtat caatgtatca tcgccgattt ggtcaatagt tgattcaaat tcttcttcat
     3421 ctaagtctcc taaagaagag ataggcaaag ttttttgcaa acgtgccaag gcttcttcgc
     3481 attcgctacg gcgttcattg tattttgaat ctgccaattc ccgccgctta ttcgtattca
     3541 tgatgacgat tgcataacca tctagtttca ctggaaccat ttcgtatttc agcgtatttg
     3601 tatctaataa gattgcatga tctagctcac cgaagccgat ggcaaactga tccatgatac
     3661 ctgaattgac accgatgaat tcattttcta cttttttacc tgtttggact agttccaagc
     3721 gatctgtttt taaatcaaac agatcttcta atacaacacc tactaataat tctaatgaag
     3781 cgctactgga aagtcctgca ccattaggga ttgttccttc cactaataac tcaaacccat
     3841 gatcgattga gtgtccagct tcttttaatt taaggaccat tccttttacg taatttgccc
     3901 agtttgcttt tttatcatat tctagctcat ctaaagtaaa actaatgaca ccgatatcgt
     3961 caaagtttgt ggaataaact aatattttat tatcgtctcg aggtgctgct acaccttgcg
     4021 ttccataagt gatggaagcg gggaagacat ggcctccatt gtaatctgtg tgttcaccaa
     4081 tcaaattgat gcgtcctggt gagaagtatt gtgtagtttt tttaggtccg aatgtttcgg
     4141 caaattttgt gtttaagctt cttttgattt ccatgatgag ttattccttt cttgttttga
     4201 ttttgaattc tgttactgtt tcaaatactg atccggcttt taaatgaatc gaaccaaatg
     4261 ctgagaagcg ctctgcacct ggagctgttt gagtctcaaa tgtgatgcca ccgtgatgtg
     4321 ccagttttcg gttgcgcatt tctggtgttt ctgtgccgaa atttgcagtg aaaataacca
     4381 cactagaagc atctgtagca atatctactt ggattctttt atcggggcta gtcaagcgag
     4441 cggcttcttt acccaagccg gtttctttta agaaaaatgg atgatcgatt ccgtcaacaa
     4501 gatttttttg atccaaatca ctagcaaaaa cggacgctaa ttttttaggt atttgaaaat
     4561 cgaaagcact tcctgcagca ttttctttta cccctatagg aatactatct gttcgcaaag
     4621 gagcataagc ttcgctattg agccaaagct catgttgatc gattgactga gaagcatctc
     4681 ccgtcaagtt gaagtaaaca tgatttgtcg gattgaataa agtatcttgg tcactggtgc
     4741 cgcgagaggt cactcgccag atattatctt ttgtcaaagt atagcggatt tccacatcca
     4801 ttgtacctgg aaaaccatgt tctccgtcgg gacttgtagt agtaaaaatg acagaagctt
     4861 cattttcgcc attcaaaatg acataagacc atttttttaa ttcaaatcca ggagcaccac
     4921 catgcagatt atgtccagtt tttggatctg tggctaattg gtaagttttt ccattgagag
     4981 aaaagcggcc attttcgatt ctccctgcgg tgcgaccgat agaagcaccg atatacggat
     5041 ctttttcgat atattcttca gcggtgtcaa atcccaaaac taattctcga tcatttgatt
     5101 ttaaactgac gattctagca cctaaatcag agatagaaag tgctagcttg tttttgttct
     5161 ccaaggttat caagtgataa ccttggccaa aatctttttc tgtaatcttc attttcaaga
     5221 tcctttctta atgggaagga actactgcat cgtgaagtta atcttccatt gtatcagctg
     5281 gagccaattt gttttccaat ttctcaacga tttgtgcatg ttttttctct gtcaatgtaa
     5341 tcttccaagc aaaaataata gcggagaaaa tcatcaaagc agcaggagca tagaatgcat
     5401 aagctttgaa agtcgttacg ccatgagcag tgatatcagc agcagtagca ttaccagtca
     5461 tcccagaagc gatagcgaca aaccctacga tcccgttaga caaagcacca gcgattttat
     5521 ctagtaaagg gcgaattgat agagtaacag cttcgttacg gataccattt ttccattgac
     5581 catattcaac agagtctgtg atcgtcatca aagcggataa gaaaatcaat tgttgaggga
     5641 agtagaacaa tactagcccg atgataacga ttggcaaact tgaacctgcg atcgtaaaga
     5701 aaaagtaaga agaaatcatc attgcgatcc ctccaaggta aacgtagcga cgagagatga
     5761 cgcgtgtaag cattgggaat agtggaacag ctactaaacc aagtaaggca gcaactagac
     5821 caacgatcca aaattcagtt ggttggccaa gtacatattt gaagaagtag aataatacgg
     5881 cagttgttgc tgcattggca atcgcataag cgatataagc aagggcaagc cacatcaact
     5941 gatcattttg agcaactgct ttgaacacat ctttgatccc agctttttca ttttgctgac
     6001 ggatcgcagt atcgacttct tttgtgccca aggcagtgat aatcgcagta ccgcctgaaa
     6061 taacagcagc aatcacaccg aaccagaacc agcctgaagc gccttcaata tgtttatgag
     6121 taacaagata ggtaaagaaa gtaacgatag ggacaacgac tagagtagta ccatttgcac
     6181 ctagagaaga gccgaaacga gcaaacgtag caaatttttc acgttccttt gtttccgtac
     6241 tcaaagctgg aatcattgac caaaaagcga tatccttaaa tgaataaaaa ctatccaata
     6301 agatgaaaac gattgcaaat aatatgataa ataaagtcgt attatgagta gctaagccaa
     6361 agaaattggt aaagattgct accaagagta cggaactaac aacgccgccg ataactaacc
     6421 atggtttgaa tttaccgtat tttgtacgag tattatcgat gatgccacca atcaaaggat
     6481 caaaaataat ttcaactaag cggataccta caactaagct tgtaactgta gcaatcattt
     6541 ttgcattcgt tgccttatct gttcctgcga acatctggct tgtcacgaat accataaaat
     6601 aagtgcttaa tgtaacatag aatgtgtcgt gtccaaaagc gcctaaagcg taggacacac
     6661 gttggatcat tttgcttttc ataatctgta ttcctctcct gttcaatata taatactaag
     6721 taaaccggtt acaacgaata acaaagatct tttaatgact taaacttaac aagaaatcgc
     6781 ttccttttta atatatttct gtgactttta tttaactttt tgttgcatta tactaaagga
     6841 gtaaggaggg atagacgtgg agcaatcgat ttttttaaaa aaggaacatc aacaagtaga
     6901 aaaagaattg tattttgatt tttgtggttt ctctaaaacg atgccgtttc attcttttgg
     6961 tcctgctatt cgcaatgatt acatcctgca tgtcgtgatg gaaggcaaag gatcttatca
     7021 tgtgaaaaat caacaattcc aattgaaaaa aggtgatctg ttcttgattc gtccaggaga
     7081 ctcgacgttt tacttatcag atggagaaga tccgtggatg tattgctggc tttcatttgg
     7141 tgggagtgct gcagaagaaa tcattcggca gtcgcttttc agagaagacc aatacacgat
     7201 ggtctcttct gacatacaaa aatatatcga cattatttta gaatgcatga aagatacgga
     7261 agaaaccttg gaaaatgaat tacagttaac agcattaacc taccgttttt tatcagtcct
     7321 tttaaaagat ggcggaaagg tcagcggtgg tgtacaaaag gttcattcct ctttagctgt
     7381 tgaaacagtc aaatacatcg aggcacatta tgctgaaaaa ttgtctgttg aggaaatcgc
     7441 ccagcattta gcggtcaatc ggagccattt gtcgcgagta ttcaaaaatc atatgggtac
     7501 cagtatcaag gaatatttga tcggggtacg gatcaatcga ggggcgtttc ttctttctct
     7561 gacggatgaa tccgtggaaa gtattgccta tcaagtaggg ttcaatagtc tagtcgtatt
     7621 tagccgaatg ttcaaaaaga gtaccggaga aacggctacg aactatcgta aacgtatgaa
     7681 aaacgaaaat tttaagaata ttgctttgga taccttgaaa aaacagttgg aaaaacaaac
     7741 cgcagtttct tggacaacct gattagtctg gttaaaatga caagcatcgg caaaagaaac
     7801 ttgtggcggt tatgctacac tgtaaagcga agactaaaaa ggaggaaaag ggatgcgtac
     7861 acttatcatc attagccatc caacgatcga agaatcttta aatcagcaat ttttcaaaga
     7921 agcttctgct ggtttaccag ctacttggca tcatttggaa tcaagttatc cagacggaaa
     7981 aatagatata gagactgaac tacagctctt ggcccagcat gatcggatca ttttccagtt
     8041 tccgttttac tggtatagtt ctcctgctca tttaaaaatt tggcaagata atgtcttgga
     8101 acgagcagca catgtattaa aagggaaaga actaggaatc gtattgacaa caggtgtggc
     8161 ggaaaaagaa taccaagctg gaggaaaaga agaatatacg atttccgaat ttttgaggcc
     8221 gtatcagcga atcgcaaata aattccatat gacctatctt ccgccattcg ttttagcaca
     8281 atttatgtat ttatcccagg aaaaacgttg ggagaagcta attgcttatc agcagtatct
     8341 ctcattggaa ggcaaaccat cgctgactca gcggattgac tggtttattc aaagagtaca
     8401 ggaaaatcag aaaatgcagg aagaagaatc tgaaaaacaa gcatatatcg tagaagcact
     8461 gactgaggca aaagagcaga tcgaagactt gacctttaca ctgcaagaaa tgaaaggaac
     8521 ttcattatga caaatgattg gctgatccaa caattagatg aattaagcga aaaatcagaa
     8581 tatcaagacc gtgcgctctt atatgaaata aagaaactga tggctgagat agacgaacga
     8641 acaatgcagc tgcaaggaga aatcgacgga caatcttgga atcatctcaa ctggtgaaat
     8701 tttcttataa ataacaaaga taaaaatgtg cacagcgaca attatttgct acaataagaa
     8761 tatgaagcgt ttgataacgc tattatacat aaaaattggt ggtgaaaaaa atgtatgatg
     8821 aaaatgagtg gggagaaaat gttcggatga cccgaagaag acagcaaaaa caggcagctc
     8881 gtaagcggat gatcatgatt ggggcagcag ttgccgtgtt tttgatcata gcaggaggag
     8941 ttgcgggtct atattttcat ggaaaaaatt cgtcagaagc gaaaagtacg accataacgg
     9001 aaacaacgac aaagaaaaca gagacgaaac aaacaagtga gtcatcaact aaaacaaaag
     9061 agacacaaac ttctgaagaa aaaacagcaa atggtttgcc agagcaactt caagcaatcg
     9121 taaatgatta ttcgaaaaag atcgaagaaa agacgcctgc attgattgaa gaataccaga
     9181 cagaaattca gggaaatcaa gagggaatag ctggtttatc agcagttgct aaccaaaaag
     9241 cacgtgagct tcaagcaatc tccgacgaag ggatcagaaa attacgtgca gcgtatcagg
     9301 cagcagaaaa taaagacggt gtagacttag atactttgat caatcaatta tcggctaatt
     9361 atacgaatca tgtcgctaag atttccgata tttatttgca gactagcgcc tctcttcaag
     9421 cagagtcaac aagcacacaa gacacaacat cttctgattc tgagacagca gagtcggtag
     9481 aagcttctga aatgcaagct cgaacaacac aagacacaac agattcctct gaaactaata
     9541 ccaacagcgc gacttcacag gctgcgacaa ccgtagtttt ggaaggagaa ggtccaaatc
     9601 aaatagccga acgaactggc gtaccagtag aaacgatctt gtcgctgaat gggatgagca
     9661 tggagaatta tttcctgact cccggtgaga cgctaaaatt gaattgataa aagcaataaa
     9721 aaagaacgaa gcttccacta aggtgaagct tcgttctttt tttattttcc ggttcttttt
     9781 tcgcgtacaa agtaatggat cgtcatgtaa agcaagaaaa cgagtccaat aatcaatgag
     9841 attctcgttt caggattgat aaacatgaaa atcaatacga tagctaaaaa agcaagtgtc
     9901 acataattcg tataaggaga tagtggcatt ttaaaaggat gatcatgcat tttatctgcg
     9961 tgcttctttt tgaagcgaat ctcgctgatc aaaatcacta cccaaggaac cataccaggc
    10021 aaaatacttg agctgtatac gaaaacgaaa atattttctg actggttgat gaacattggt
    10081 agaagataat ttaagatcag accaattaag attccacctg agattgctac gacaggataa
    10141 aaaggcgcac cgtgacgaga tagtttcaga aattttttag acatctttcc atttatggca
    10201 agtgtataga ccatacgact tgcgctgaaa atccctgaat tgcaacctga taatgccgct
    10261 gtaatcacga caaaattaat cagtcctgca gcaaatgtaa tgccgacttt agaaaaagtt
    10321 tgtacaaaag gtgaaccaat ctcactcaat tgattccacg gatagatact cacaatgata
    10381 aaaatagcac caatataaaa aatcaagatt cgaccgatag tggactggac agcttcaatg
    10441 atcgtctttt taggattctc tgcttcacca gcagtcattc cgatcagttc gattccttga
    10501 taggaagcaa caacgatcga taatgcaaag aaaaatcctt tgacgcctcc agtgaagaat
    10561 ccaccatttt tccaaagatt tgaaatccct actgcctgac catgattacc aaatccaaat
    10621 aagattaccc caagacctgc aatgatcatc aaaatgatcg ttatgacttt gatgatcgaa
    10681 aaccaaaatt ctaattcgcc aaacgccttg acagaaataa gatttgccag cataagaaac
    10741 aagacgacaa tgattccggg aataattgcc ggcaaatcag gccaccaata attcatataa
    10801 gatcctacag caatcacttc acttattcct acgacaatat attgaaaaat attactccaa
    10861 gcggtcaagt agccagccac aggatggatg tactcgctcg cgtaatttgc aaaagaacca
    10921 gtcgaaggat caacgtaaag catttcgcct aaagcacgca taatcaaata caaaaagaat
    10981 cctgcaatcg cataggctcc catcactgag ggccctgtcc aacgtatggt tgaggaggca
    11041 cccataaaca gaccgacacc gatcgttcca cctagtgcga tcatttggat atgacgggca
    11101 ctaagacttc gagttaactt ttcttcttcc atacaaaaac tcctttcgtt gttgagtcaa
    11161 caagaaaaaa taatcaaacg tttcaacaca tgtttttcat tattaggtta gccttattga
    11221 actcttgatg tgattctaca gaaaattttc ataaaatgcg aggaaaattc gaaaaaaact
    11281 ttttcatcat tctacttgaa aatgctttcc attttagtta aactatattt aaataaaaag
    11341 ttaggtaaac ctaaaaagga gtgtaagatt tggaacgtac ggccattgta attcaagacc
    11401 tttcagccgc ttatcaaggg aaaactgttt tgaaaactat caacttgacg ataaatccac
    11461 aaaaaattac cggtattatc ggaccaaacg gtgcaggaaa atcaacattt atgaaatctt
    11521 tgctagagtt gataccagct actactggta aagtcacctt ttctggacag tcggtaaact
    11581 cagtccgtaa aaaaatagcc tacgtggaac agcgaagtga attggatctt tcctttccaa
    11641 ttgatgtatt aggcgttgta cttttaggaa catatccatc tttacgaatt ggacaaagac
    11701 ctgggaaaaa agaaaaagaa cgtgcaagac aagctttgaa aaaagtaggg atggaagaat
    11761 atgccaaaag acagatcagc gaactatcgg gtggacagct tcagagagtt tttattgcaa
    11821 gagctctagc ccaaggagca gaatggatct ttttagatga accattcgta gggattgatg
    11881 cgttaagtga acgaaagatc tttgacatct tgcaggaatt gaagaattca ggaaaaacga
    11941 ttttgatcgt ccatcatgat cttcataaag tagaagaata tttcgatgag gttattcttt
    12001 taaataaaca gctgatcgct tccggtccag tacaagaagt ctttacatcg aagaaccttc
    12061 aattggccta tggtgagatc atcagacatt tagtaaaagg aggagagaaa aaatgatcca
    12121 atcctttatt gatggattaa tgaattatca attcctgcaa tacgctttgg tcacttccat
    12181 gcttgtagga cttgcttctg gagtcatcgg ttcttttatt attttacgag ggatgtcttt
    12241 gatgggagac gctatctctc acgcagttct tccaggtgta gccatttctt atatgtttgg
    12301 ttttagttac atctttggag ccactgcttt tgggatgcta acagcagcag tgattggttt
    12361 cgtgactcag cggagccgat tgaaaaatga tacagcgatt ggaattgtct ttagctcctt
    12421 ttttgcatta gggattatct tgatttcata tgcccagagt gcgacagatt tgtatcatat
    12481 tttatttggg aatgtcttag ctgttaggga aagcgatttg ttgctgacag cactcgttag
    12541 tggaatcgtc ttgatatttg tctttttctt ttataaagaa ttgaagatca catcattcga
    12601 tccgacaatg gcaaaagctt atgggctgaa cacatcgctg atccattatc ttttgatgtt
    12661 ctttttgaca ttagtggctg tagtcagttt acagacagta ggaacaatct tggtgattgc
    12721 catgttgatc acaccagccg ccacggctta cttgctaacg aaccatttac tgaaaatgat
    12781 cattacagct gcaggaatcg gtatgctaag tgcagttgtc ggtgtgtttt tcagttatag
    12841 ttacaattgg ccatcaggag ctacgatcgt gttagcatgt accgcatttt ttatccttgc
    12901 ttttttattt tctccaacaa aaggaatttt atttcagaaa ggaagcagat agatgaaaac
    12961 aaaaaaatca ctctttttaa tcttggcagt tagtttcttg gtattagccg gttgcggaaa
    13021 gcaggcttct gaccaagcgg atgaaggaag taaagaaaag ttgtcagtcg tggctaccaa
    13081 ttcgatcttg gcggacatgg caaaagaggt cggaacagat caaatagata tccacagtat
    13141 cgtaccagtc ggaacagatc cgcatgaata tgaagtacta ccagaagaca tcaaaaaggc
    13201 aagcgatgca gatgttattt tatacaacgg tttgaatctt gaaacaggta acagctggtt
    13261 cgataacttg atggaaacgg ctaaaaaaga agaaggaaaa gattattttg cagttagcaa
    13321 aaatgtagaa cctctttatt taactagcgg tgaagaacat acaaaagcag atccccatgc
    13381 ttggctagac ctatctaacg gaataaaata tgtggaggaa atcgcacgta tattctctga
    13441 aaaagatgca gaaaatgcga cactctataa aaaaatgcag aagcatatgt ggaaaaacta
    13501 aaagaattag atacacaagc gaaggaaagt tttgcttcta tcgaagagaa caaaaaatta
    13561 ttagtaacaa gtgaaggggc attcaagtat ttttcacgag catatgattt gccagcagct
    13621 tatatatggg agatcaatac agaaagtcaa ggtacgcctg atcaaatgaa agcgatcatt
    13681 gatcagataa gagcaaaaga agtaccggtt ttattcgtgg aaaccagtgt agactcaaga
    13741 agcatggaac gggtagcaaa agaaacgggt ttgaaaatct atgataaact gttcactgat
    13801 tccatagcaa aagaaggaga acaaggagat tcttactatc agatgatgaa atggaatatt
    13861 gaaacgatcc atgaaggttt gtcacaaaca aaagaaagct gaaaagtgca aggtctagct
    13921 tgaggagcag accttgcatt ttttataaga gatatgaaaa aaaattatgc tgtttttcgt
    13981 cgtacagata ccttttttga agaaattccg ttatagttag agtaaggaag gaaggtgtgg
    14041 ctatgaattt tacacgtaaa gataaatata cgattaaaga agaagtcttt cgattgatca
    14101 gccagcagga aaataccgac aaggatcaag accttcaatt tcttgaagca gaattagcta
    14161 aattattcat agcaaatcaa aatgagacaa cagaaccaga tgaagagctg tacataggca
    14221 aatggttgtt tgatgaattt cagcctttag atgaaaatac cggtatcggt tattttgtgt
    14281 tcctttctcc agtcggcttc acacctggcg tattagttaa acaaaacgac gaaataaccg
    14341 tcaaagcatg gcctgcatta ttttcagtca tcaaagcaga aggagtaatc aaaaagatcc
    14401 agtcggccta ttataaaagt caatttattg ccaacagcaa attaatgaat atgttttata
    14461 atcgtctagt agaaattgct aggcctggga aaatcccgca aacattcacg tacacacagc
    14521 aaaaaaatct ctatgaagaa gtattgtatt cccgcgatct gtctgagttt tcgccatggg
    14581 aagaggtaag gctccatgaa ctgttaagta caaaaccagt ttcattgaat ttttttaatg
    14641 cattagataa caaaagaaaa ttaatttcaa caaattattg ggaagtattt gctgcaagaa
    14701 aaaaatggat ggaagaaaag aagattgccg atcagcagat ccagtttgtc cagctgaaaa
    14761 aagaaaaact atattatggg ttatacagcc gttaacagca agcgaggaag accatgacat
    14821 taaaagaaga aatcatacaa gaaagcaaac gattaggaat tgacaaaatc gggttcacca
    14881 cggcggaacc ttttgattca ttaaaagaat cgttggaaga acaaaaagca gctggccata
    14941 cttctggctt tgaacatcca aatatcgatg agcgtcttta tccagaaatg acatttgatc
    15001 agccgaaatc gatcatttcc attgcacttg cgtatcctac taggatccat gaagaagtgc
    15061 ctagggatga aaaacgcggg cagtttgctc gtgcttcttg ggggatcgat taccatgata
    15121 tactcagaga ccgtttgaac cgattgatcg aattcatcca aacaagagca gaaaaatggc
    15181 aaaaagaaga ggaatggcgt ctagctccgc aagtagatac tggagaactg atcgatgtcg
    15241 ctgtagcaca gcgagctggt ttaggattta ttggacgaaa cggattgttg atcactgaag
    15301 agttcggttc atttgtctat cttggtgaga tcgttactaa tattgtattt gagcctgata
    15361 aaccaggaga atttggttgc ggtgactgta tgcgctgcgt gactgcttgt ccgacaaaag
    15421 cattgcttgg tgacggaaga atgaatgccc agcgatgttt gtcttatcaa actcagacaa
    15481 aagggatgat gccggaagaa tatcgtaaaa aaatgcgaaa tgtcatttat ggatgtgata
    15541 tctgccagtt agtctgccct tacaatcaag gaaaagattt tcattttcat gaagagatgg
    15601 aaccaaaagt agacgaaata tatccaaaat taaagccaat gctttcgatg tctaataaag
    15661 acttcaaaca gcaatttggg catttagctg gttcatggcg tgggaagaaa cctttgcaga
    15721 gaaacgcatt gattgcttta gctaatctag gtgatcgaac ggcattgcct gagatttcag
    15781 aatgtttaac agatgtccgc cctgtcatca gaggaacagc agcttgggct attggacgtc
    15841 taggaacaaa agaaccggaa aaatggcttg atgtattaga tgaaatatta gctaaagaaa
    15901 cagaagaagt cgttataaac g
//
LOCUS       DAYYAO010000061        14994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000061 DAYYAO010000000
VERSION     DAYYAO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.064"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACHARI_001922"
     CDS             <1..608
                     /locus_tag="ACHARI_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442958.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ACHARI_001923"
     CDS             628..1656
                     /locus_tag="ACHARI_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8442959.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ACHARI_001924"
     CDS             1653..2411
                     /locus_tag="ACHARI_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8442960.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ACHARI_001925"
     CDS             complement(2497..3237)
                     /locus_tag="ACHARI_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8442961.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ACHARI_001926"
     CDS             3298..4551
                     /locus_tag="ACHARI_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8442962.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4846..5100
                     /locus_tag="ACHARI_001927"
                     /pseudo
     CDS             4846..5100
                     /locus_tag="ACHARI_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ACHARI_001928"
     CDS             5222..5548
                     /locus_tag="ACHARI_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8442963.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ACHARI_001929"
     CDS             5616..6329
                     /locus_tag="ACHARI_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8442964.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /gene="licT"
                     /locus_tag="ACHARI_001930"
                     /pseudo
     CDS             6639..7491
                     /gene="licT"
                     /locus_tag="ACHARI_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7612..9477
                     /locus_tag="ACHARI_001931"
     CDS             7612..9477
                     /locus_tag="ACHARI_001931"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8442965.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9491..10934
                     /locus_tag="ACHARI_001932"
                     /pseudo
     CDS             9491..10934
                     /locus_tag="ACHARI_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11042..11743
                     /locus_tag="ACHARI_001933"
     CDS             11042..11743
                     /locus_tag="ACHARI_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8442966.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11869..12381
                     /locus_tag="ACHARI_001934"
                     /pseudo
     CDS             11869..12381
                     /locus_tag="ACHARI_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12779..12988)
                     /locus_tag="ACHARI_001935"
     CDS             complement(12779..12988)
                     /locus_tag="ACHARI_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8442967.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13229..13804
                     /locus_tag="ACHARI_001936"
     CDS             13229..13804
                     /locus_tag="ACHARI_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8442968.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13804..14244
                     /locus_tag="ACHARI_001937"
     CDS             13804..14244
                     /locus_tag="ACHARI_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8442969.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14260..14961
                     /locus_tag="ACHARI_001938"
     CDS             14260..14961
                     /locus_tag="ACHARI_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8442970.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5066 a         2485 c         3161 g         4282 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    13141 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    13201 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    13261 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    13321 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    13381 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    13441 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    13501 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    13561 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    13621 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    13681 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    13741 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    13801 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    13861 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    13921 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    13981 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    14041 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    14101 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    14161 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    14221 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    14281 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    14341 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    14401 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    14461 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    14521 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    14581 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    14641 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    14701 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    14761 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    14821 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    14881 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    14941 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa aatt
//
LOCUS       DAYYAO010000062        14441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000062 DAYYAO010000000
VERSION     DAYYAO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.065"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHARI_001939"
     CDS             complement(227..1114)
                     /locus_tag="ACHARI_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8442971.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHARI_001940"
     CDS             complement(1111..1980)
                     /locus_tag="ACHARI_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8442972.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHARI_001941"
     CDS             complement(2117..3403)
                     /locus_tag="ACHARI_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8442973.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHARI_001942"
     CDS             3676..4254
                     /locus_tag="ACHARI_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8442974.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHARI_001943"
     CDS             4254..5183
                     /locus_tag="ACHARI_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8442975.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHARI_001944"
     CDS             5180..6916
                     /locus_tag="ACHARI_001944"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8442976.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHARI_001945"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHARI_001945"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8442977.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHARI_001946"
     CDS             complement(7812..8321)
                     /locus_tag="ACHARI_001946"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8442978.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHARI_001947"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHARI_001947"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8442979.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHARI_001948"
     CDS             complement(9622..10713)
                     /locus_tag="ACHARI_001948"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8442980.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHARI_001949"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHARI_001949"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8442981.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHARI_001950"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHARI_001950"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8442982.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHARI_001951"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHARI_001951"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8442983.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4113 a         3144 c         2610 g         4574 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt a
//
LOCUS       DAYYAO010000063        14149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000063 DAYYAO010000000
VERSION     DAYYAO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.066"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..859)
                     /locus_tag="ACHARI_001952"
     CDS             complement(35..859)
                     /locus_tag="ACHARI_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8442984.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(832..1512)
                     /locus_tag="ACHARI_001953"
     CDS             complement(832..1512)
                     /locus_tag="ACHARI_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8442985.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1535..2473)
                     /locus_tag="ACHARI_001954"
     CDS             complement(1535..2473)
                     /locus_tag="ACHARI_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8442986.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2718..3569)
                     /gene="ispE"
                     /locus_tag="ACHARI_001955"
     CDS             complement(2718..3569)
                     /gene="ispE"
                     /locus_tag="ACHARI_001955"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8442987.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3723..6296)
                     /gene="mprF"
                     /locus_tag="ACHARI_001956"
     CDS             complement(3723..6296)
                     /gene="mprF"
                     /locus_tag="ACHARI_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8442988.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6437..7243
                     /gene="proC"
                     /locus_tag="ACHARI_001957"
     CDS             6437..7243
                     /gene="proC"
                     /locus_tag="ACHARI_001957"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8442989.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7322..8878)
                     /gene="rny"
                     /locus_tag="ACHARI_001958"
     CDS             complement(7322..8878)
                     /gene="rny"
                     /locus_tag="ACHARI_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8442990.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9289..10338)
                     /gene="recA"
                     /locus_tag="ACHARI_001959"
     CDS             complement(9289..10338)
                     /gene="recA"
                     /locus_tag="ACHARI_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8442991.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10447..11688)
                     /locus_tag="ACHARI_001960"
     CDS             complement(10447..11688)
                     /locus_tag="ACHARI_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8442992.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11928..12506)
                     /gene="pgsA"
                     /locus_tag="ACHARI_001961"
     CDS             complement(11928..12506)
                     /gene="pgsA"
                     /locus_tag="ACHARI_001961"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8442993.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12715..13638)
                     /locus_tag="ACHARI_001962"
     CDS             complement(12715..13638)
                     /locus_tag="ACHARI_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8442994.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         4046 a         3150 c         2472 g         4481 t
ORIGIN      
        1 ttttcgtttt tataaggctt cttttcttta taatttagtt attttttctt ctgaaggcaa
       61 ccaatattcg tttgatgata tttacaatca ggaacaaagc aataaagact aatgtgattg
      121 ttgctcctgg aggtgtatct agataaaacg agctagttag tcctgacagc atccctagaa
      181 atcctacaca aacactgata gcaatcacga cattgaagct tttgcctaat cgcataccga
      241 ttgctgcggg aagaaccatg attgcggata tcaaaagtgc accggcaatc gggatcatca
      301 cagcaatagc cacaccagta atcacattga acatcatcga catcaaatga acgggtagcc
      361 catccacgtg ggcggtatct tcatcaaaag tcagaacata catcggccgt ttgaataaaa
      421 agaacaatat accgatggca acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa
      481 tggtcacgat cgaaccaaac agataggatt ggatacttgt tgctgaattc cctccgctca
      541 aattcatcaa tactaacgct agcgccaaac caccagccat taaaatggca atggagattt
      601 ctgaataggt tcggtagatc gttcgcagat actccaatat cacagctgct aatacaacga
      661 ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa tcctaacgct acccctgcta
      721 aagagacatg agaaagcgta tcagccatca gcgattgtct tctgatcact aaaaacacac
      781 ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt
      841 catatgaaag caacgccatt gagaatcctc ctttctcacc agtcggatct gtctgtccgc
      901 atactgtttg atatcttcat gatcatgggt gatcattaga actgatttcc catggacatg
      961 cgcactatgt cggagaagac gataaaattc atttctggat tcttcatcca tacctgttgt
     1021 tggttcatca agaacaaata agtcagggtc agtagcaaaa atacgcgcta aactgattcg
     1081 ctgcttttgt ccgccagaca attcacctat tcgccgattt cgcatatccc acatccctac
     1141 agcttctaaa gcacgctgta catgttgatg atctttttca gagagacgct tgaaccagcg
     1201 gcctcttgga tatcttcctg aacggactaa ttcgatcacc gtactaggaa aacctgcatt
     1261 gaaagatgca acttgttgag gaatataacc gatacttaat ttttttcctt cttgattttt
     1321 ctcagcaatt tttatcattc cttttgaagg tttcagtaat cctaacgatg ctttcaccaa
     1381 ggttgacttg gctgctccat tttctccagt taaaatgaca aattcacccg catctacatg
     1441 ataagagaca tcttctagta caggttcatc atcatagtaa aaggtcaaat cagatatttc
     1501 gatataacgc accttacaac ctccattcta aaacttaatt gatactttct tttaaagctt
     1561 tcagattttt ttccatgaca gaaatataat tttcgcctgc tttgatttgt tcattagtca
     1621 agctttctaa aggattcaac acttctagct tcactcctgt ttcattcgac aatgtctcag
     1681 ctaccttcga agaggcattt tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa
     1741 attcttttag ctcagctaag cgacttggtg tgggttcctg atctggtgaa acgcccgaaa
     1801 tcgctacttg ttttaagtcg tattgattcg ctaaataagc aaaagcagca tgctgagtga
     1861 caaatgtgcg gtttgttgca tcttttagct catcggagta tttttgatcc agtgcttcaa
     1921 gctttttgat ataagcatcg gcattcttcg taaaagtttc ctttttatct ggatattttt
     1981 cgcctaattc ttcagcaatc gttctgactt cttttatagc taataccgga tcgagccaaa
     2041 catgtggatc gtattcatgt gaatgaccat gcccttcttc tgattcttca tgttcgtctg
     2101 cttcttgcga ttctaaacgg tcgattcctt tagctgcttc gatcatcaag gtctgtttgc
     2161 tatccactga atcctttaac gaatccacaa aaaattccat gtctgaacta ttatacacga
     2221 aaacatctgc atcggaaatt tctgctcttt cttttgcact tggttcgaaa tcatgcggct
     2281 ccgttccagc agggatcaaa agtttcacgt ttccttcatc ccctacgatc tcttttgtaa
     2341 aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct
     2401 tgttcgtatt tccacatgct gcaaacagta aagcacctat cattaccgta gctgtcaaaa
     2461 gatatttttt catttatatg ctcctttcaa tgaacaaatc ggaattttac gatttattaa
     2521 cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag aaatccatca atttctgatt
     2581 taagcatgta tattcttgat taaatcgtaa aatggatgag ttgctgctca tatctagcca
     2641 tttcactaca ataaaaaact gaagaatacc attttaggat tcctcagttt ttcatcacct
     2701 attttacttc ttatatttta ttgcagggtt cgtactaaat aaacttcctc acaaaaacct
     2761 tttaacccat tatatactct ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt
     2821 ggtccgcttc cactcatcaa tgcagcatct gctccgtact tgatcatacg ctctttgatt
     2881 tgatggatga ctggatgccg ctttcccgtt atatcttcta aactgtttcc catttttttg
     2941 atcatctgct gataatcttg ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt
     3001 tggatggctt gcgtatctac ttctctaaac actgtacgtg tagagacact cattcttggt
     3061 ttgaccaaca caacccagca ctgtggcata gaaggtaata cttggatttc ttcccctttg
     3121 ccaccgacaa atgccgtact tccgtagaca cagtaaggaa catctgtacc gacttccaaa
     3181 ccgatttctg ccagttcttc taacgaacag cccagattcc ataattggtt gatccctctt
     3241 agagcagctg cacagtctgt acttcctccg cctaatccag ctgctactgg aatattcttc
     3301 ttaacaaaga tcttgacacc ggttttgata ttgtatttat tttttagcaa taaagcagct
     3361 tgataaacat tgttccgttc atctaccggt aaaaatgctt tatttgtttc tacaatgatt
     3421 ttgtcttctg ttatttcttc aaaaaataga tgatctgcca agtctacgct cgacatcacc
     3481 atttccaact catgataccc atcttgccgt ctatataata catccagtcc taaatttatc
     3541 ttggctggcg ctttttcaat caattccatc ctctcaccca ttcgaaaagt ctaagtcgca
     3601 agtattctat catagggaaa aataaaagta tagcttatgt ctgcctagtg gttgattttg
     3661 tcatcgtttc aacagctttc acagaatttt ctcttcataa tccaaacaag ttctagtgat
     3721 tcttataatt ctgtgagcac ttcttctgcc aaactcggaa catttccttt ttcttttcct
     3781 atcagactga gtagctgcat ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa
     3841 taacgtcctt cccaatgatc gacatatttt aatttctttt cataagcaga ctggaaggaa
     3901 tagaaaaaat taccataatt atatacgata tgcatgactt tttcttgaaa gaaagaaaaa
     3961 tctgtttctc caacattagc taatggagct aagcctagat tggcttccga gtagcccatt
     4021 ttctcgtata caataaacag attggctaac agatagtcag ctaattcctc agattcttta
     4081 ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg tcatcggctg agcggtgatg
     4141 aagccaacga ctttttgatc tttacggaca ataccgacac cgctagataa tacatatgat
     4201 ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa
     4261 acttcattta cagcttgatg taattcttcc ggcagttgat cgtaccaagt aaaatggtat
     4321 ccttctttca tcaaacgttg atattcaagt tgctgactca gccgagacaa gtcttctgtt
     4381 gctagatcaa caatccctgc ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc
     4441 atcactacgt agctttctga tatcttataa aacgcaagct gatagcctaa acaatctgct
     4501 tgtttcataa actgattcgt tgctgcccgc atcattttag gattgccaac tggatctcct
     4561 aaaacaaagc atttgtttcc tttgatctga taaccaaaaa cgacttgatc ttggtcgtct
     4621 tcttggtaat aaaaataact gtaacctgac aagcgaagct gatggcttgt agctgtgcct
     4681 ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt actcactgcc aagcttcgta
     4741 gtatcttcag ctaaatattg ataaagcgcc actaaagcta gcaatgagat gcccaaacca
     4801 ataagccctg agaaccacac atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt
     4861 cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag
     4921 ccgaacaagc tagcatcaaa gattatagct ccccaagaat aaacgaattt tgttcgataa
     4981 aattcttttc tggataacca cacagctaac agaatcaaga catataccaa tatcaactgc
     5041 caagataacg tacgtgtgat tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc
     5101 agaaatgcct ttttgacttt tactgaaagt gctctagcaa ggccgagtaa taaaaagcca
     5161 accaatagat tcactgtctg atctaaaaaa ttgaatgaga acggcaataa tacttgaaat
     5221 agccgactga ttgcagacag attagtgatt gttgatagaa gtaccatcat gatccccgca
     5281 aaatagacag cacttacgag cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg
     5341 ttatctaaaa atcgattgat tttgactcct gtatgtgtga tgaacaatag aagtcccgtc
     5401 ataaaaggta gaacataata aaacaaccga taatataata gccaaactac tgctacatct
     5461 tgccgaacag gcaattgact cattcctaag atcatcaaaa cgtcaaaagt ccccattcca
     5521 cctgggacca tcgtcagcat tcctatcaat gtagcaatca tgaacatagg atagatcgaa
     5581 agcatcgaaa tatctattct catcaacttg ccgatgatca aaaataccgc aagggctccc
     5641 gtccattgtc caaaggatgc cagtagcaga ccaagcaagc cttttggata aaactcgaag
     5701 aacaatgtct ttctctttag ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg
     5761 ccgaaaagcc agatccaata gcttcgaaaa atactttctg actgaataaa aaagacatca
     5821 ataaatgtaa gaaacgacca tacagacaaa ccagtcagca taaatagtgc gacttttgat
     5881 acagtggcta aaaccatttt tctatttgtc tgctttccat aaaaactagc ccgcagactt
     5941 gctcctacca caccaccaaa accggcgaga ttgttgatcg tgtttgttac ccacgcagca
     6001 agaagaaact cttttctttc catttttggt tttccttgtt tttctaagac tctgatcgtc
     6061 acccaatcat acaacaacat cggagtgaca cccagcagtc cagccagaat catgagcagg
     6121 atcgtcatgc ggctttgttg tcccatcctt tgccaaatat cttgccaact catgccatta
     6181 gcaatatttg cgacttgatt tgccacaaag actaacacgg agccgaaaaa aatcagtttt
     6241 aaaatcagac tatgttcctt tatccattgc agcatcaagt atcgtttatt tttcacattt
     6301 tcccattcct aacttgtata cttttgtgtc ataccccaat gttatcttat ttattttaat
     6361 ttttccactt tagacctgtt ctttgcttgc gatgatagaa aaagaaagat atcctaatgc
     6421 taaaggagag gataacatga aagtaggatt tatcggcaca gggaatatgg cacaagctat
     6481 tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg
     6541 acattttgaa aatgcacaaa aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa
     6601 tcaggaagtt gcatcacaat cagatatcgt tcttttagcc gtgaaaccaa aaatcattga
     6661 aaaagtattg gaagaactaa gagaatatag ccagaaagtc ttattcattt ctattgcttc
     6721 aggaaaatca ttaacagatt tagaaacagc gcttgaagat cctcaggcct ccatcgtacg
     6781 tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga
     6841 agctgccacg gaggaacagc ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt
     6901 ctatccttta gcagaatcag atttcagtac attcattggc cttgctggca gctcacccgc
     6961 attcgtttac atgctgatcg atgcgatggg acggactggt gtactgaatg gtttgtccaa
     7021 acagacagcc actgagattg ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc
     7081 aagtgataaa aatccatggg aacttgtaga ccaagtttct tctcctggcg gtacaactgt
     7141 tgcaggtgta gttgccttag aagaagcagg attcatccca gcaatcatca aaggaatcaa
     7201 tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa taatgatagc aatgtttttt
     7261 cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg gctcttcttt tgtatttttt
     7321 attatttagc ataatcagtt gcacgagttt cccgaataac gattactttg atgtgtccag
     7381 gataatccag ttcttcttcg attcgtttac gaatatcacg tactaaacga acagcgtcta
     7441 aatccgagat ttcttctggc ttgaccatta cccgaacttc gcgtccagct tgaactgcgt
     7501 aacttgattc tacaccggcg aagctgttcg aaatattttc cagacttctt aaacgacgga
     7561 tgtagttttc taatgattca cttcgcgcac caggtcttgc tgctgagagc gcatctgctg
     7621 cagctacaag gacagagata acagaagtag cttccacatc tccatgatga gaagcgatcg
     7681 cgttgattac tactgggttt tctttgtact tggcagctaa ttccgcaccg atctcaacgt
     7741 gagatccttc aatctcatgg tcaagtgctt tcccaatatc atggagcaac ccggcacgtt
     7801 ttgctaattg gatatcttcg ccaagctctg ctgctaagat accagctaat tttgcaacct
     7861 cgaccgaatg cttcaagacg ttttgtccat aactagttct gaatcgcaga cgtccgatga
     7921 tcttgattag atctggatga agtgtatgag caccgacttc aaacgctgcc tcttccccgt
     7981 attcgcgaat acgttcatcc atttctttac gagatttctc gaccatttct tcgatacgcg
     8041 ctggatggat tcgtccatct tgtatcagtt tttccaaggt catccgtgcg atttctcttc
     8101 ggattggatc gaatccggaa agaacaactg cttccggtgt atcatcgatg attaaatcga
     8161 taccagtcag cgtctcaagc gttcgaatat tacgaccttc tcgtccaatg atacgacctt
     8221 tcatttcatc attcggaagg gtaacgactg aaacagtcgt ttctgaaact tgatctgctg
     8281 cgcagcgttg aatcgctaat gataataagt ttttcgcttt gcgatctgct tcttctttgg
     8341 cacgctgttc agattctttt accattagag ttaactcatg attcagctct tcttcagtcg
     8401 atttcatgat gacatctcgt gcgtcctctt ttgataaacc agcaattttt tctagttctt
     8461 tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt
     8521 ttgaactaag tgcttcttct ttactttcaa gtgagttctc gcgtttttct aacgagtcat
     8581 ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta
     8641 acttactttc tctcagctca ctttcaactt cagaacggta tttctgattt tcttccttag
     8701 cttctaacaa tgcttctttt tttagagttt ctgcttcttt ttttgcacta tcgatgattc
     8761 cagcagctga tgattgtgca ccagctattt ctttctcgtg acgtgacttt gcgaccataa
     8821 accctaaacc aagaccgaca attaaaccga tgatagcgag gagaatgttg aataccataa
     8881 aatccacctc catactatct ttttcatttt tatcatctat acagcttcta tgataaatta
     8941 ttctaaactc gtcaatatgc atcttcgcat atatgtgtac aaaaaacatg cacaacttta
     9001 ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac aaagaaacgg ctttctactt
     9061 attcattaaa cgatggtttt taaaataaat gacactagtt tcatataggt aaacctttaa
     9121 attgagaaac ttttctatta aattattctt tgtagatgaa cagatatttt tattttatgt
     9181 acccactcgt ataattaaat aactcacgat ttctttattc aataaaaaaa cggcaggtca
     9241 aggagagtac ctactcttca gaccagccgt ttttgtttat cataattttt attcttctac
     9301 taaaggcaat tcttcttgtc ctttttcatc ttctgggaca gcgacttctt caccaatacc
     9361 ataagctgct ctaactagag cagaaacttc tgccatcatt tcaggatgat tagccatata
     9421 gattttagcg ttttcccgtc cttgaccaat ccgatcttct ttataagaat accaagcacc
     9481 gcttttatca acaatgtctt tttctactgc catgtctaga agttctcctt cttgtgagat
     9541 ccctaaacca tacatgacat ctacttcagc gattttgaaa ggaggtgcta ctttgttttt
     9601 tacgactttg atctttgtac ggttaccaac aatatctgta ccttgtttca attgttctgc
     9661 acgacgtact tccaaacgga tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat
     9721 ttctggattc ccaaacatca cgcctacttt ttcacggatt tggttgatga aaatagcgat
     9781 tgtttttgtt ttgttgatcg aaccagagag tttacgcaat gcttgagaca tcaaacgtgc
     9841 ttgtaacccg acatgtgagt cacccatttc gccgtcgatt tctgctcgtg gaactaaagc
     9901 agcaactgaa tcgactacta cgatatctac agccccactt gatactaaag catcagcgat
     9961 ctctagacct tgttctcctg tgtcaggctg tgaaagaagt agttcatcga tattcacacc
    10021 taatttttgt gcatattgcg gatctaacgc atgctcagca tcaatgaaag cggccgttcc
    10081 gccatttttt tgtacttctg caatagcgtg tagtgcaact gttgttttac ctgaactttc
    10141 aggtccatat acttcgatga tacgtccacg ggggtaaccg ccgacaccta aagcgacatc
    10201 taacgcaaga gagccacttg gaattgtaga gatttgttgg tcgatttttt cgcccaattt
    10261 cataatagag cctttaccat agctcttttc aatttttttc agtgcagcat ctaaggctgc
    10321 tttacgatca tctgccaata cgataatcct ccttatattt acaaatcaga gcaacatttc
    10381 attatcctta tcatatcttt tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg
    10441 catttatcat tttcttaaaa gctgttttct caaaagatca aatccttgca taaccgcatt
    10501 atatctgata gcagcacgat ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt
    10561 agatgaaata gcgatccata ctgttcctgc agattgacct tctaacgtgt ccggtccagc
    10621 cactccggtg aaagctacac tgtaatctgt tcttgctatc tcacgtgctt gaatggccat
    10681 ctgctccaca cattctttgc tgactgttcc atattctgct aatagctccg gatcaatctc
    10741 cagaaaacca gcttttgttt ggagcgagta ggtaacgaat ccgcctggaa atacttcaga
    10801 tacaccagca atgtcgccta atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc
    10861 tgttacagtc tgcttgtttt ctttcagtag ttccactacg acttttgcta gcgaattatc
    10921 atcgccgtat ccataaaaat attcccctac tcgttcttgg attttttctt ctaatgctaa
    10981 aagagcttga ttccctgcct gaacatcatt tgttttcact gtcagtcgta atgtcacttc
    11041 atttggtttg gcatatggtg cgatcgttgg attgatctgt gtctcaatca gatcttttag
    11101 ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc aaaactttag aaattagttt
    11161 ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact tgttccacaa acattggttt
    11221 caactcatta ggcggaccgg gaagaaggat atacgcatgt gtatctgttt gataaaaagt
    11281 ccctaaagca agccctgtac gatttggtag aggaaccccg ccttcgataa tctgggactg
    11341 ttgcagattg tttttggtca tttttcgatg ggttgtttca aaatatgcca ataatttttt
    11401 gtatccttcc gtattttgta tgagagactt accgaggtga gccgcagttg cttcttttgt
    11461 caggtcatct tctgtcggtc ctaatccgcc acataagagt atgagttcac ttcttgtttc
    11521 agctagagca atcaactctt ctaaacgctg ctgattatct cctacaactg tttgataata
    11581 aacttcgata cctagatcag ctagctgttc tgacaagaaa gtggcattcg tattcacgac
    11641 ttgtcctagt aaaagttctg ttccgacagc tattatttct gctttcatta cttcccgctc
    11701 ccttatatag tatacgctgt atttttatat ttttttattt aaaaagaggg caaggagctg
    11761 tgacaaaaat cttgtcacag ctccttgttc caaataaaca gtgttctcct gccaaaacct
    11821 cggtcataag tcacaatata cataaaacat gaaaagctgt tttttgaaat tatcccttgt
    11881 gactcagagt tagacggcag gctcacaacc tctccactaa acgatactta cattgatcct
    11941 ttgaacacac cgctgttttt gatgaagtaa tccacacctg aatagatagt aaagatcaga
    12001 caagcataaa gcatgatttg atctactgga ataccgatca ggctgaatgg gatattattg
    12061 ataaacagca agataatagc aaccatttgt gtagccgtct tgacttttcc cggccaagct
    12121 gcagccatga cttctccatg ttcgacaagt agtaaacgca agcctgtgac ggctaattca
    12181 cgacagacaa tgatcgctac tacccaacca ggggcctttc cttggtcaac caacatgata
    12241 aaagcagtca taactagcat tttatctgcc aacgggtccg caaacttgcc aaaattagtg
    12301 acaaggcctc gcgcacgggc aatttttcca tctaaccagt ctgtgatact agctaaagca
    12361 aaaataactg cacctactag ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt
    12421 ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg ggatcataca aattctaagt
    12481 acagttaatt tatttggtaa attcacacta tcgactcctt tcttccctat tctacgtgac
    12541 tatcagcgaa gtttccaccg atattattta cttttccaaa tgaatttatt caaaggagta
    12601 tttttattga aattgatctt tttgaattaa cgaagaagga ccgcgacaca gatttgtcac
    12661 gatccttctt ttttatacct acaagtaaac agatgcgtaa actaatttat acgatcattc
    12721 tgcgtattga agagtaaggt tcacattttt tggactctga ttgttttccc ctggattaac
    12781 tggtacggat tctccattcg ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt
    12841 cgcatttgtt gctgtttccg gtaacacggt actctgtgtt tcatttgctg ccagtgtccc
    12901 ttgataaaca taggcattgt ctactagcac acctaaccat acacgattga cagcagtaaa
    12961 ttcaagggtt acaggcttgg tagcgtctgt gatatttact gcgatggcac tgccagaatc
    13021 ttctcctgta gctattgcca ttttcttttc ttcacttgat tttgttgttg attctactgt
    13081 agaagactct ttggtctgcg tgctttgctc tgaggaagag gactcctcga ctgatccttc
    13141 tactttaagc gaagaagctg tgtctccgat tattggatca gcttgacgat cttgccaagt
    13201 catatagccc acgacaacga caatcgccaa agcaaccaat cctaataaaa tgacaggaag
    13261 acttgtccaa aagcgacttg gatgcgattc ttcctgatgc attgcttttc tagaagtact
    13321 tactgtttca ggctgcggac gttcagggac cgatcctgtc aatttgcttt tcccatcaaa
    13381 aacatcgact aaatggtctc catcttctcc tacagcttca gcgtattgcc gaataaatgc
    13441 ccgcacgtaa aatttaccag gcaattggtc aaaatcatca ttgtcgattg cttccaaata
    13501 acgtttttga atttttgtaa tctgctgtaa ttcatctaaa gatatttttt tattcaggcg
    13561 tgcttgccgt aatttttctc cgatggtttc aaaagccact cgttcatctt ctccagtctg
    13621 tttgattctc cgaagcatct gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca
    13681 aaagtcatgt cacagctcct tttttccgaa taaacattgt tctcctgcca actcctcggt
    13741 cacaagtcac aatatccaat aaatataaga agctgttttt tgaacttgtt ccttgtgact
    13801 cggagttaaa cggcaggctc acgacctctt tcttaaacgg tgttcaatca gctgttcctc
    13861 ggtattagct caaaaaccgg caaaatataa ggagatattt ttccgtatgt ttctctaata
    13921 ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    13981 tgcggtaata agtccaacca agttaaaaac attaagaagc tgtttttact tggctgttct
    14041 tattacttga tgcagggcag ctttttcaca acctcttggt acacggtgtt ccagaagtag
    14101 cttcttcggc aataagccga aatatcacaa aaatttgaga agcaatttt
//
LOCUS       DAYYAO010000064        13917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000064 DAYYAO010000000
VERSION     DAYYAO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.067"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHARI_001963"
     CDS             131..505
                     /locus_tag="ACHARI_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8442995.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHARI_001964"
     CDS             complement(619..1248)
                     /locus_tag="ACHARI_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8442996.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHARI_001965"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHARI_001965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACHARI_001966"
     tRNA            1668..1741
                     /locus_tag="ACHARI_001966"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACHARI_001967"
     CDS             complement(1879..2043)
                     /locus_tag="ACHARI_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8442997.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACHARI_001968"
     CDS             2338..3666
                     /locus_tag="ACHARI_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8442998.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACHARI_001969"
     CDS             3868..4701
                     /locus_tag="ACHARI_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8442999.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACHARI_001970"
     CDS             4717..5454
                     /locus_tag="ACHARI_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8443000.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACHARI_001971"
     CDS             complement(5609..6577)
                     /locus_tag="ACHARI_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8443001.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACHARI_001972"
     CDS             6809..7642
                     /locus_tag="ACHARI_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8443002.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACHARI_001973"
     CDS             7676..8515
                     /locus_tag="ACHARI_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8443003.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACHARI_001974"
     CDS             8541..9101
                     /locus_tag="ACHARI_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8443004.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACHARI_001975"
     CDS             9300..11021
                     /locus_tag="ACHARI_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8443005.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACHARI_001976"
     CDS             11186..12328
                     /locus_tag="ACHARI_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8443006.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACHARI_001977"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACHARI_001977"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8443007.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAYYAO010000065        13840 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000065 DAYYAO010000000
VERSION     DAYYAO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.068"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..134
                     /locus_tag="ACHARI_001978"
     CDS             <1..134
                     /locus_tag="ACHARI_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8443008.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            456..626
                     /locus_tag="ACHARI_001979"
     CDS             456..626
                     /locus_tag="ACHARI_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443009.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            640..1242
                     /locus_tag="ACHARI_001980"
     CDS             640..1242
                     /locus_tag="ACHARI_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8443010.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1258..1935
                     /locus_tag="ACHARI_001981"
                     /pseudo
     CDS             1258..1935
                     /locus_tag="ACHARI_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2246..2743
                     /locus_tag="ACHARI_001982"
     CDS             2246..2743
                     /locus_tag="ACHARI_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFM8443011.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2744..3160
                     /locus_tag="ACHARI_001983"
     CDS             2744..3160
                     /locus_tag="ACHARI_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFM8443012.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3180..4724
                     /locus_tag="ACHARI_001984"
     CDS             3180..4724
                     /locus_tag="ACHARI_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8443013.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4867..5091
                     /locus_tag="ACHARI_001985"
     CDS             4867..5091
                     /locus_tag="ACHARI_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443014.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5106..5975
                     /locus_tag="ACHARI_001986"
     CDS             5106..5975
                     /locus_tag="ACHARI_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8443015.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5956..6690
                     /locus_tag="ACHARI_001987"
     CDS             5956..6690
                     /locus_tag="ACHARI_001987"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8443016.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6723..7631
                     /locus_tag="ACHARI_001988"
     CDS             6723..7631
                     /locus_tag="ACHARI_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8443017.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7628..8170
                     /gene="sat4"
                     /locus_tag="ACHARI_001989"
     CDS             7628..8170
                     /gene="sat4"
                     /locus_tag="ACHARI_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM8443018.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8263..9057
                     /locus_tag="ACHARI_001990"
                     /note="aph(3')-IIIa"
     CDS             8263..9057
                     /locus_tag="ACHARI_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8443019.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9333..9464
                     /locus_tag="ACHARI_001991"
                     /pseudo
     CDS             9333..9464
                     /locus_tag="ACHARI_001991"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9540..10033
                     /locus_tag="ACHARI_001992"
                     /pseudo
     CDS             9540..10033
                     /locus_tag="ACHARI_001992"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10203..11099
                     /locus_tag="ACHARI_001993"
     CDS             10203..11099
                     /locus_tag="ACHARI_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8443020.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11198..11407
                     /locus_tag="ACHARI_001994"
     CDS             11198..11407
                     /locus_tag="ACHARI_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8443021.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11424..11696
                     /locus_tag="ACHARI_001995"
     CDS             11424..11696
                     /locus_tag="ACHARI_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8443022.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            11698..12561
                     /locus_tag="ACHARI_001996"
     CDS             11698..12561
                     /locus_tag="ACHARI_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8443023.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(12824..13561)
                     /gene="erm(B)"
                     /locus_tag="ACHARI_001997"
     CDS             complement(12824..13561)
                     /gene="erm(B)"
                     /locus_tag="ACHARI_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8443024.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(13686..13769)
                     /locus_tag="ACHARI_001998"
     CDS             complement(13686..13769)
                     /locus_tag="ACHARI_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8443025.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         4909 a         2152 c         2866 g         3913 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc atactatgat acgtgtttgt ttttctgtgc tgtttagtga
      181 atgattagca gaaatataga gagtaagatt ttaattaatt attaggggga gaaggagaga
      241 gtagcccgaa aacttttagt tggcttggac tgaacgaagt gagggaaagg ctactaaaac
      301 gtcgaggggc agtgagagcg aagcgaacac ttgatctttt aagttgctat ctttttaagg
      361 tcaatagagt atacttattt gtcctaatat ggttttagca gtataattga cttggtgaat
      421 aggtcattta agttgggcat aataggagga gtaaaatgaa aaaatttatt tatcgagttt
      481 tagaaaatga cgaagtggtg gctattttta atgagcaaca atatgcgcaa gattttatcg
      541 cttacgaaaa gacaatttct gataagcaat ttgaaattga aaaagtagat atagctgatt
      601 ggttattgca accgagagaa ttttagaggt tggttgaaaa tggctaaaat tggttatgca
      661 cgtgtcagta gcaaagaaca gaacttagat agacaactga aagcgttaga gggcgtttct
      721 aaggtctttt cagacaaatt aagcggtcaa tcggtcgaac gcccacaatt acaagctatg
      781 cttaactata ttcgtgaagg ggatatagtt gttgttactg aattagatcg attaggacga
      841 aataataaag aattaacaga attgatgaat caaattcaaa ttaagggggc aaccctggaa
      901 gtcttaaatt taccctcaat gaatggtatt gaagatgaaa atttaagacg gctgattaat
      961 aatttagtga ttgaattgta taagtaccaa gcggaatctg aacgcaaacg aattaaagaa
     1021 cgccaagccc aaggaattga aattgctaag aaaaaaggaa aattcaaagg gcgacaacct
     1081 aaattcaaag aaaatgatcc acgtttacaa cacgctttcg atttattttt gaatggttgt
     1141 tccgataaag aagttgaaga acaaactgga attaatcgcc gaacgtttag aaggtatcga
     1201 tcaagataca acgtgacagt agatcaaaga aaaaaacaat gaaaagaggg atagttaatg
     1261 agtacggtta ttttagctga aaaaccaagc caggcattag cctacgcaag tgctttaaaa
     1321 caaagcacca aaaaagacgg ttattttgag atcaaagacc cactatttac agatgaaacg
     1381 tttatcacct ttggttttgg gcatttagtc gaattagcag aaccaggtca ttataacgaa
     1441 aagtggcaga actggaaact tgaatctttg ccgatttttc ctgatcgata cgattttgaa
     1501 gttgcaaaag ataagggaaa gcagtttaaa attgttgctg aacttctcaa aaaggcaaat
     1561 acaattattg tcgcaacaga tagcgacaga gaaggtgaaa atatcgcctg gtcgattatc
     1621 cataaagcaa atgccttttc taaagataaa acgtataaaa gattatggat caatagctta
     1681 gaaaaagatg tgatccgaag cggttttcaa aatttgcaac caggaatgaa ttactatccc
     1741 ttttatcaag aagcgcaaac acgccaaatt gccgattggt tgatcggcat gaacgcaagc
     1801 cctttatata ccttgaattt acaacaaaaa ggcgtacaag gtacattttc attaggacgt
     1861 gtacaaacac cgactttata tctcatttat caacgccagg aagcaataga aaactttaaa
     1921 aaagaacctt ttttcctgaa taattcatag ttttactaat agtctttcct aacattgatt
     1981 taatagtctt taaagtattc tgcttcatca cccattaggt gatgaaaaaa gaaccccttt
     2041 ctgatttagc ggacaaatac tttgacctaa tctcagaaga tataaaagaa aaaataataa
     2101 aatatccgat ttatactttg tataaagaac taaagtagtt agcaaatcat ttacagcaaa
     2161 tcactatgtt ccacatacca ttttacatac ttaccttatc cacgacacat gttgagatag
     2221 tatcgaagat aaagaaagtt gctccatgtt gtttttgcga gatggggaaa tcaaagccac
     2281 attgacaacc atgatgaata agcttgcttt cagtcataag ctaatcctag aaccactttt
     2341 caagtcagtt agccaaatcg atgaagaaag cgactgtgaa agaatggacg ctattgataa
     2401 gctaatggag caactattgg aagaacgcaa tacccttatt gcactgatgt ccaaaggttt
     2461 cctagaacct gctcttttta atcaggaacg aaatgtttta gatagtgaga ttaaaaatct
     2521 tactaccgaa aaaacaaacc tggtaacgaa ctctgcgagt ggggttttgc gagcaaacga
     2581 gataaaggac ctcattaatt atgtgtcagc agataatttt aatggtgact acacggaaga
     2641 actatttgaa gaatttgtag tgaacatcat tgtaaattcc agggatgagc tgacattcaa
     2701 tttgaaatgc ggtctttccc tgaaagaaaa ggtggtgaga taaatggcat atattccata
     2761 tggatacaaa attcaagatg gagtggttac tgtcgatgaa aaggcagcag gtcaagtaaa
     2821 ggtattcttt gagaaataca tatcaggact atcccttaca gtggctggcg aacaggcagg
     2881 tattgaaaag acacattctg tgatggggcg cattttgaaa aacgtcaact accttggaaa
     2941 tgatacgtat ccagcaatta ttgataaaga gatatttgat aaagctgaag aagttagaga
     3001 taaacgtgca aaggatttag gacgagtggt agagcttgcc gctttcacct ctccccctcc
     3061 caaagaacga ttcaaaatga aaaaggcaga taataagatg ccagtggatc cttttgaacg
     3121 agcagaatac ttatacagtc tgatagaaag cgaggaataa agtgacagag aaaaatataa
     3181 tggttattcc tgctcgtaaa agagtaggaa gtacagccgc aaaagaaaag ataaagaaac
     3241 ttcgtgttgc tgcctattgc cgtgtttcta cagaaacaga agaacaaaat tctagctatg
     3301 aggttcaggt ctcacactat acagagttta taaagaaaaa taatgaatgg gagtttgctg
     3361 gcatctttgc agatgatggt atctctggta cgaacactaa aaagcgtgac gaatttaatc
     3421 gcatgattgc agagtgtatg gacggtaaca tcgacatggt tattacaaaa tccatcagcc
     3481 gatttgcgcg taacacccta gactgccttc aatacattag acagctcaag gataagaaca
     3541 tatcagttta ttttgagaaa gagaacatca acaccacgga tgccaaaggt gaggttttgc
     3601 tgactattat ggcatctttg gcacagcagg aaagccagag cctttcacaa aacgttaagc
     3661 ttgggctaca gtaccgatac caacaaggaa aggtgcaggt caaccataag cgatttatgg
     3721 gctacacgaa agatgaagat ggaaatttaa ctattgtccc cgaagaagct gagattatca
     3781 aacgcatcta ccgagaatac cttgagggtc agagtctagt gggcattggt cgagcccttg
     3841 aaaaggatgg tattttaaca gcagcgggaa aaccaagatg gcgaccagaa tcagttaaga
     3901 agatacttca aaacgaaaaa tacatcggag atgcccttct gcaaaagact gtcacagtag
     3961 attttctgac caagaaacga gtgaaaaatg aagggcatgt tccccaatat tatgttgaaa
     4021 atagccatga agcgattatt cctaaagagt tatttttgca ggcgcaagaa gaaattcatc
     4081 ggagaagcaa tatctacaca ggagaaggca agaacaaaag aatttacagt agcaagtacg
     4141 ctttaagtgc catcaccttc tgtggagatt gtggcgatat ttatagacgg gtctattgga
     4201 atatccacgg aagaaaagaa tttgtctggc gatgcgtgac tagaatcgag caaggtcctg
     4261 aagtttgtaa gaaccgaacc gtaaaagaag atgaacttta tggtgcagta atgactgcga
     4321 ttaataagct acttgcaggt gggaacaata tgataaagac actggaagag aatattcatg
     4381 ctgtgattgg cgaaaccaca gaataccaaa tttcagagat taacaactca ctagaggaaa
     4441 aacaaaaaga actaatcaag ctggcgaaca agggtcaaga ctatgaccac ttagcagatg
     4501 agattgatga gctgagagac aagcgacaga tccttttagt agaagatgcc tccctcagtg
     4561 gcgagaacga gcgaatcaat gagctgattg aatttatccg caagaacaaa ttccgcacct
     4621 tagaatatga tgataagctt gtaaggaaga taatccagag cgttacagtc tatgaaggcc
     4681 acttcgtcat agccttcaaa tctggcatcg aaatggaaat atgaaaacca gaagtaaata
     4741 gcccatgact ctacagtaga gttgtgggtt ttcttttgct tgctgattat aataatattt
     4801 taataactat tgtgtttatc aaccaaatgg tttataatat tcttagcgta aacttggagg
     4861 tgctgtatga ctacggcaga aatgattaaa gaactgtgtg agcaaatgaa tataagtgtt
     4921 tccgaacttg ctagacgtat tggccagact ccacagaatt tcaataaaaa attacaacga
     4981 gaaacagtaa ccttggatga gttgaaggcc attgctgatg tgctaggtgt caagtttgtg
     5041 caggcattta ttttaccaga tggagaagaa ataaaaatat ctaacgaata aaggaggatg
     5101 tttatatggt aggtaatgtt attcaaatag taacagagaa attatcatct ttgcctttta
     5161 tagaaggcat cgtattaggg ggctcacgtg caagaggcac ccatacagaa aattctgata
     5221 tagatatcgg aatctattac aattcagact catttgacct gacagcgatt aatcaaattg
     5281 ctacagagtt ggatgatgag aatagaaaca accttgttgt acctcccgga gcgtggggtg
     5341 attgggttaa tggcggtgga tggttagtta taaacgggtg tcatgttgac ttgatattac
     5401 gtgatataaa acgggtggaa caaataatca aagatacgga gcaaggaatt gttactgcca
     5461 attatcagac cgggcatccc catggttata taagtgctat gtatcgtgga gaattagcga
     5521 ttagtaaaat actatatgct aagaacgaaa gcttatgcga attaaaaaag caggcagaaa
     5581 tttaccctac tgctctaaag aaaagcttga tgaacttttt tatatttgaa gctgaattct
     5641 ctttaatgtt tgtaaaagca aatgcggggg cagaggataa atattatatt gcaggccatg
     5701 tttttcgtat tatttcatgc ttaaatcaag tactatttgc atgtaataat gcttattgca
     5761 ttaacgaaaa gaaagctata aaactgcttg aaacttttga atataaacct gaaaaatatg
     5821 ccgaaagagt taatcatatt tttgaagtac tcggtctttc tctttttgaa tgctacgaca
     5881 tgaccgagaa actttataaa gaagtgaatg aaattgtatc ggagataaat aactttttaa
     5941 acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac
     6001 agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa
     6061 aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg
     6121 cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat
     6181 aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt
     6241 gccatagaag atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg
     6301 tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct
     6361 ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac
     6421 tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag
     6481 ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc
     6541 acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag
     6601 ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg
     6661 ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt
     6721 aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac
     6781 gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat taatatacct aaagatgaat
     6841 ttcaggatta tgatattaca tattttgtaa gtgatataga accgtttata tctaatgatg
     6901 actggcttaa tcaatttggg aatataataa tgatgcaaaa gccggaggat atggaattat
     6961 tcccacctga agaaaaggga ttttcctatc ttatgctatt tgatgattac aataaaattg
     7021 atcttacctt attgcccttg gaagagttag ataattacct aaagggcgat aaattaataa
     7081 aggttctaat tgataaagat tgtagaatta aaagggacat agttccgact gatatagatt
     7141 atcatgtaag aaagccaagc gcaagggagt atgatgattg ctgcaatgaa ttttggaatg
     7201 taacacctta tgttattaaa ggattgtgcc gtaaggaaat tttatttgct attgatcatt
     7261 ttaatcagat tgttcgccat gagctgctga gaatgatatc atggaaggtc ggcatcgaaa
     7321 caggctttaa attaagtgta ggcaagaact ataagtttat tgaaaggtat atatccgagg
     7381 atttgtggga gaaacttttg tccacctacc ggatggattc ctatgaaaac atatgggaag
     7441 cattatttct atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc
     7501 attatgccta tccggagtat gataggaata taacaaaata taccagggac atgtataaaa
     7561 aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc
     7621 gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga
     7681 accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac
     7741 agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga
     7801 tgaggaggca gattgccttg aatatattga caatactgat aagataatat atctttacta
     7861 ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta
     7921 tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat
     7981 caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca
     8041 ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt
     8101 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
     8161 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
     8221 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
     8281 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     8341 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     8401 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     8461 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     8521 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     8581 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     8641 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     8701 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     8761 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     8821 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     8881 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     8941 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     9001 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
     9061 ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta atcttttctg
     9121 aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa gttcgctaga
     9181 tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt
     9241 ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca taggatatac
     9301 tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga gtaacctcta
     9361 ttcttattca gctgcaatca aaaaaaataa ttcctgccaa agaaattgca cagcgattta
     9421 atataagctt aaggacagtt tacagagata tccggacact tgaagaagcc ggaataccaa
     9481 ttggatctga ggccggaaaa ggatattttc ttgtagaggg cttcctactt ccgccggtaa
     9541 tgtttacagc ggcggaagtg ggtgcattga ttacagcagg gaaattttta aattgccatg
     9601 gagatgaatc atttataaag gattttgatt cagctatgta taaaatcaaa tctattttga
     9661 agcatggtga aaaaaactat gcacaggagt tggagaacag tattaacgtg tacagcacat
     9721 ctggacagaa aaatacattg gcggataacg ttattgcagc aattcagact gcaatctgca
     9781 ataaaagggt aatatcaatt caatatcctg catcaggagg gcaagaaccg gaaagcagaa
     9841 tgatagagcc tatatcactg gggttttatg aacagaactg gtacttaatc ggttttgcag
     9901 gctaagaaat gaatatagga attttcgtgt ggatcggatt aaaagcattt gcatagaaga
     9961 agagctctgt caaagccatg atggctcttt ggaacaaata ttaaaacaaa tgctttcata
    10021 taaaaaacta tatgccctac aatgccgttg taaaaattgg ggaaaaggga tatattgatt
    10081 ttatatcttt ctcaaaataa atataaaagc cctttaaaga gggcttttat atattaatca
    10141 caaatcactt atcacaaatc acaagtgatt aatcacaaat cacttgtgat ttgtgattct
    10201 taatgataca atattactat acaaaaaaag aatggggcgt agttatggag aaggaaaaac
    10261 taaaaatact tgaagaatta agacgtattt taaacaataa aaatgaagca attattatct
    10321 tgaataatta ctttaaaggt ggtgttggaa agtccaaatt atcgactatg tttgcttact
    10381 tgacagacaa attgaattta aaagttttaa tgatcgataa ggacttacag gcaacattga
    10441 caaaagactt agcaaaaact tttgaggtag aattgccacg tgtcaatttt tatgaaggct
    10501 tgaaaaatgg aaacttggct tcttctatta ttcatttgac tgataattta gacttgatcc
    10561 ctggcacgtt tgatttgatg ttactgccaa aattaactcg ctcatggacg tttgaaaatg
    10621 aaagtagatt gcttgctact cttttagcac ctttaaaaag tgactatgat cttattatta
    10681 ttgatactgt accaacgcca agcgtttata caaataatgc aatcgtggcg agtgattacg
    10741 ttatgatccc tttacaagca gaagaagaaa gtacaaacaa cattcaaaac tatatttcct
    10801 atttgattga tttgcaagaa cagtttaacc ctggactaga tatgattggt tttgttcctt
    10861 atttagttga tacggacagc gcaacgataa aatcaaacct ggaagaactg tacaaagaac
    10921 ataaagagga taatttggtt ttccgaaata ttatcaagcg aagtaataaa gtaagtactt
    10981 ggtctaaaaa tggcattaca gaacacaaag gctatgacaa aaaagttttg tctatgtatg
    11041 agaacgtatt ttttgaaatg attgagcgaa tcattcaatt agaaaatgaa aaagaataga
    11101 atcacaaatc acaagtgatt aatcacaaat cacttgtgat ttgtgatagg tgatgataaa
    11161 ataaatagta agaagaaata gaaagaagtg agcgatcgtg ggaaatttag gcgcacagaa
    11221 agcaaaacga aatgatacgc caatcagcgc aaaaaaagat ataatggggg ataagacggt
    11281 tcgtgttcgt gccgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa
    11341 cgtaaaagaa gttatggatc aagccttaga agaatatata cggaaatatt tacctgacaa
    11401 actttaaaag gagtgaaaat gaaatggcag ttacgtatga aaaaacattt gaaattgaaa
    11461 tcattaatga gttatcggca agtgtttata atcgggtatt aaattatgtt ttgaatcatg
    11521 aactagatac taaaaatact cgtttactag aagtgaatct tttaaatcaa ttagaagtgg
    11581 cacaagaagt tgatttattt caacaaccat ttgaagaatt acaagctatt catgagtatt
    11641 ggcggtcaat gaatcaatat tcaaaacaaa ttttgaataa agagaaagtg gcttaacatg
    11701 gcgaatatta ctgacttcac cgaaaagcaa tttgaagatc gtttagaaaa gaatgttgaa
    11761 cgactaacta aaaatagact agcggttgaa tcgccaaccg cttttttact tggtgggcaa
    11821 ccagggtcag ggaaaaccag tttgcgatca gcaatttttg aagaaacaca agggaatgtt
    11881 attgtcattg ataatgatac ctttaaacaa cagcacccaa attttgacga attagtgaaa
    11941 ctctatgaaa aagacgtagt aaaacacgtt accccttatt cgaatcgcat gacagaagca
    12001 cttattagcc gtttaagcga tcaaggctat aatttagtga ttgaaggaac aggacgaaca
    12061 acagacgttc ccattcaaac agccacaatg cttcaatcta aaggttatga aacaaaaata
    12121 tacgtcatgg cagtaccgaa aattgaatca tacttaggaa cgattgaacg atatgaaacc
    12181 atgtatgcag atgatccaat gacagctagg gcaacaccaa aacaagcgca tgatattgtt
    12241 gtcaaaaact taccgaccaa tttagaaacc cttcataaaa cgggcttatt tagcgatata
    12301 aggctttaca atagagaagg agtgaaactc tattcaagct tagaaacgcc ttccattagt
    12361 ccgaaagaga ccttagaaag agaattaaat cgtaaagtat cagggaaaga aattcaaccg
    12421 actttggaga gaatagagca aaaaatggtt caaaatcaac accaagaaac ccctgaattt
    12481 aaagcaattc aacaaaaaat ggaaagcttg cagctaccta caccaccaat acccaaaaca
    12541 cctaaacttc caggtattta aaccttaaaa aaggaaaaga gtagttacca aaaaacggta
    12601 actactcttt ttttataaaa acatttcctc atgttggggt cccgagcgcc tacgaggaat
    12661 ttgtatcgat aagaaataga tttaaaaatt tcgctgtttt tttctaattt cattttgagg
    12721 tgaattaatt ggtagttatt agaggtgccc tatacctaat aatttatcta cattcccttt
    12781 agtaacgtgt aactttccaa atttacaaaa gcgactcata gaattatttc ctcccgttaa
    12841 ataatagata actattaaaa atagacaata cttgctcata agtaacggta cttaaattgt
    12901 ttactttggc gtgtttcatt gcttgatgaa actgattttt agtaaacagt tgacgatatt
    12961 ctcgattgac ccattttgaa acaaagtacg tatatagctt ccaatattta tctggaacat
    13021 ctgtggtatg gcgggtaagt tttattaaga cactgtttac ttttggttta ggatgaaagc
    13081 attccgctgg cagcttaagc aattgctgaa tcgagacttg agtgtgcaag agcaacccta
    13141 gtgttcggtg aatatccaag gtacgcttgt agaatccttc ttcaacaatc agatagatgt
    13201 cagacgcatg gctttcaaaa accacttttt taataatttg tgtgcttaaa tggtaaggaa
    13261 tattcccaac aattttatac ctctgtttgt tagggaattg aaactgtaga atatcttggt
    13321 gaattaaagt gacacgagtg ttcagtttta atttttctga cgataagttg aatagatgac
    13381 tgtctaattc aatagacgtt acctgtttac ttattttagc cagtttcgtc gttaaatgcc
    13441 ctttacctgt tccaatttcg taaacggtat cggtttcttt taaattcaat tgttttatta
    13501 tttggttgag tactttttca ctcgttaaaa agttttgaga atattttata tttttgttca
    13561 tgtaatcact cctgaagtga ttacatctat aaataaatac agaagttaaa cgatttgttt
    13621 gtaattttag ttatctgttt aaaaagtcat aagattagtc actggtagga attaatctaa
    13681 cgtatttatt tatctgcgta atcactgttt ttagtctgtt tcaaaacggt agatgtttta
    13741 tctacattac gcatttggaa taccaacatg acgaatccct ccttcttaat tacaaatttt
    13801 tagcatctaa tttaacttca attcctatta tacaaaattt
//
LOCUS       DAYYAO010000066        13827 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000066 DAYYAO010000000
VERSION     DAYYAO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.069"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACHARI_001999"
     CDS             168..362
                     /locus_tag="ACHARI_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443026.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACHARI_002000"
     CDS             631..930
                     /locus_tag="ACHARI_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443027.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACHARI_002001"
     CDS             complement(1027..1461)
                     /locus_tag="ACHARI_002001"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8443028.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACHARI_002002"
     CDS             complement(1693..2403)
                     /locus_tag="ACHARI_002002"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8443029.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACHARI_002003"
     CDS             complement(2393..3253)
                     /locus_tag="ACHARI_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8443030.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACHARI_002004"
     CDS             complement(3258..3896)
                     /locus_tag="ACHARI_002004"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8443031.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACHARI_002005"
     CDS             complement(3911..4210)
                     /locus_tag="ACHARI_002005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8443032.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACHARI_002006"
     CDS             complement(4253..5737)
                     /locus_tag="ACHARI_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8443033.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACHARI_002007"
     CDS             complement(5757..6218)
                     /locus_tag="ACHARI_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8443034.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACHARI_002008"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACHARI_002008"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8443035.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACHARI_002009"
     CDS             7521..8291
                     /locus_tag="ACHARI_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8443036.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACHARI_002010"
     CDS             complement(8564..9391)
                     /locus_tag="ACHARI_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443037.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="ACHARI_002011"
     CDS             9959..11194
                     /locus_tag="ACHARI_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8443038.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="ACHARI_002012"
     CDS             complement(12193..12453)
                     /locus_tag="ACHARI_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8443039.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="ACHARI_002013"
     CDS             complement(12480..13781)
                     /locus_tag="ACHARI_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8443040.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4447 a         2829 c         2176 g         4375 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgac
//
LOCUS       DAYYAO010000067        13808 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000067 DAYYAO010000000
VERSION     DAYYAO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.070"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHARI_002014"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHARI_002014"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8443041.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACHARI_002015"
     CDS             complement(2261..2512)
                     /locus_tag="ACHARI_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8443042.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACHARI_002016"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACHARI_002016"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8443043.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACHARI_002017"
     CDS             3462..3680
                     /locus_tag="ACHARI_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443044.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="ACHARI_002018"
     CDS             complement(3969..4481)
                     /locus_tag="ACHARI_002018"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8443045.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACHARI_002019"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACHARI_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8443046.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="ACHARI_002020"
     CDS             complement(6934..7377)
                     /locus_tag="ACHARI_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8443047.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="ACHARI_002021"
     CDS             complement(7378..8166)
                     /locus_tag="ACHARI_002021"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8443048.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACHARI_002022"
     CDS             complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACHARI_002022"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8443049.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="ACHARI_002023"
     CDS             complement(9281..9868)
                     /locus_tag="ACHARI_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8443050.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACHARI_002024"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACHARI_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8443051.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="ACHARI_002025"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="ACHARI_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8443052.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="ACHARI_002026"
     CDS             complement(12785..13720)
                     /locus_tag="ACHARI_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8443053.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DAYYAO010000068        13738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000068 DAYYAO010000000
VERSION     DAYYAO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.071"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..607
                     /locus_tag="ACHARI_002027"
     CDS             <1..607
                     /locus_tag="ACHARI_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443054.1"
                     /translation="SLEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYR
                     GERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIA
                     SLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINK
                     ALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            662..1828
                     /locus_tag="ACHARI_002028"
     CDS             662..1828
                     /locus_tag="ACHARI_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443055.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            2245..5472
                     /gene="fss3"
                     /locus_tag="ACHARI_002029"
     CDS             2245..5472
                     /gene="fss3"
                     /locus_tag="ACHARI_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8443056.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            5584..5898
                     /locus_tag="ACHARI_002030"
     CDS             5584..5898
                     /locus_tag="ACHARI_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8443057.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5911..6285
                     /locus_tag="ACHARI_002031"
     CDS             5911..6285
                     /locus_tag="ACHARI_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8443058.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            6295..6639
                     /locus_tag="ACHARI_002032"
     CDS             6295..6639
                     /locus_tag="ACHARI_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8443059.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            6708..7724
                     /locus_tag="ACHARI_002033"
     CDS             6708..7724
                     /locus_tag="ACHARI_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8443060.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            8318..10192
                     /gene="ltrA"
                     /locus_tag="ACHARI_002034"
     CDS             8318..10192
                     /gene="ltrA"
                     /locus_tag="ACHARI_002034"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8443061.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            10294..10674
                     /locus_tag="ACHARI_002035"
                     /pseudo
     CDS             10294..10674
                     /locus_tag="ACHARI_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            10719..11861
                     /gene="dcm"
                     /locus_tag="ACHARI_002036"
     CDS             10719..11861
                     /gene="dcm"
                     /locus_tag="ACHARI_002036"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFM8443062.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            11851..12140
                     /locus_tag="ACHARI_002037"
                     /pseudo
     CDS             11851..12140
                     /locus_tag="ACHARI_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12396..13580
                     /gene="mobT"
                     /locus_tag="ACHARI_002038"
     CDS             12396..13580
                     /gene="mobT"
                     /locus_tag="ACHARI_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8443063.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            13577..13690
                     /locus_tag="ACHARI_002039"
     CDS             13577..13690
                     /locus_tag="ACHARI_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8443064.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         4697 a         2326 c         2920 g         3795 t
ORIGIN      
        1 tagtttggaa tttactatag ggggcgttac tttgaataga ataaaatttt atattgattc
       61 cagtaaaaag accattaaac aaattagtac agaaactggt attaactata caactcttac
      121 gaactattac cgtggtgagc gaaatcctag aaatactgaa acatggaata tccttgctga
      181 ttatttccat gttcctgtaa gttatttgat gggattagac gaaacaaatt ccatagaaca
      241 acctaatctt gatagaataa aaactgctcg tttagaaaaa aaggacaaaa tagtcaagaa
      301 tattgccagt cttgactcag attattttac tgaattatct aatctatact tttccatgac
      361 ttctttactt gaagtcaaac ttcaagaaga tgactccgac tttctttttt taaaaagtat
      421 tctagaggac tcctttgaat acacgattct atttgccaaa caaaagaagt ttattaataa
      481 agctttaaac ttaaatatcg atgaaagaaa agagttagaa tctgagttat ttggcaactt
      541 aacccaagta caggttaaca atctaaaaag agagataaca gctcgattaa tatcaaaact
      601 tcaataaaac taattattca atcagccaac tcgccaagtt atcagattgg agagattagt
      661 tatgtcaata caaaagtaca caacaaaaag cggtgcacga tatagtgtcc gcttgtatgt
      721 tcgtaaagat gaaaatggaa agcaagaata ctatgtaaaa aaagggttta aaactgaaaa
      781 agaagccaag ttacacgaag caagaaaaaa ggtcgagatt gaagataccg gctatcataa
      841 gccttctaat gacttgttta taacagtgta tgaagaatgg ttgccttctt ataggaatcg
      901 tgtacaagaa acgacatatc aacgtacaaa agaccttttc cgcttgcata tactaccaaa
      961 attcgggaat aagtccattt ctaagattac tcctgttcac tgtcagaatg cagtcaatga
     1021 atgggcttta aaattcaaga atatcaaaca gcttaagtct tacagctctc aaatatttga
     1081 gtatgccatt tttgcagagc tattacaccg aaatcctatg gcaaatacaa atctaccaaa
     1141 gcgggaaaag aaagaaaatg aaaattactt ttcattagaa gaactaaaag ctttttggga
     1201 tattctcaat aaagaagagc ctttaaaaca tattttaatc ttccagcttt tgattactac
     1261 tggtatacgt aaaggagagc tagctgctct tcattggtct gacatttatt ttgaagaaca
     1321 gctaatgtat gttagaaaga gctatgcaac tattcgaaat gaagagaaga atgcaagaag
     1381 aaaaactgtc aggattcaaa aaaatacaaa aaatatatct tccgaacgaa tattaccaat
     1441 tgatgcacaa actattgaaa tgttaaaaat ctggaaaaaa gaacaagctc gagagctttt
     1501 gcagtttggc ataaatacaa atacaaaaaa tcaacttatc tttacctatg taaatgcaga
     1561 cggtgacatt aatcaacctt tgcatgcgga ctattcaaat aatatattga aacgtttaga
     1621 gaagaaatat aaatttaagc acgttactat tcatggattc cggcacactc acgctactct
     1681 tctactagaa ggtggagcaa gtatcaaaga aacacaagat cgattgggtc ataaaaatgc
     1741 tgaaactacc cttaacactt attctcatgt tactgaaaaa gctcaacgaa atgcgttaga
     1801 aaaattcatt gcttacactg gtttataata ttgtcattac ctttctcatt accagaacga
     1861 aaaaaagaca ccttctcgca atgagaaagt gtctgaaatc gttggtataa caacgattaa
     1921 cgttttgaaa actgtgaagc tttacgtgct ttcttaagac ctggtttttt cttcgtatca
     1981 ttgaataagt ttgaaaagcc ttgatttata aaggtttttc cgtaaatata ttttgttata
     2041 tttgattaaa tttgaaatgt ttgtgttcta aaatgtgttc taatttaaag gcagtatatt
     2101 tcgtatttta tattaaattt ttaggctcat tcattctcaa atccattcta tttagtcaat
     2161 aaatatttta aaaagacttt ataaacaaag atgttcaagc gagcatctta tttttttgtg
     2221 cctaatttga aaggagaaca tttcatgtat aaaaaacaaa aaaagaaatt atgtgtatta
     2281 actattttga gtttggctgt tcaatcaatt gtaggagcaa ttgctcctac gattgctttt
     2341 gctgatgaaa ttacccatcc acaaacggta accgtagaac tagatttagc acatcaatat
     2401 gctgttgaag gaacttttag tgatggtcgt ccaatgtcag aagtcactgt tccgcattat
     2461 gcagtgtaca acggcgttaa acaagatatt ttctgtgttg aaccaggagt cccgatttac
     2521 aatgagttca ctcctggata tgagaagaac ccgttaccag atatgtccga taaagccaaa
     2581 ttagtttccg ttttatggaa aaaagtaggt acagatgcag atactcatat tgtggcacag
     2641 aagatgatct ggcaagaagt taatgggtat acccttcact caataaaacg atcagatggt
     2701 agtgccgtaa atatcgcagc cattgaggca aaaatcaatc aagccattgc cgattatcag
     2761 aagaaaccaa gcttccataa tagtacggct aaaacagtat tagggcaatc gactactgtg
     2821 acggatacga ataatctgaa tttgtcagag ttcgatgagg ttgtggaaaa taccgcaaac
     2881 attgattatc gtgtgaatgg caatcagttg ataatcagcc caaatgccaa ttcaaaagaa
     2941 agtggtgtgt tgacgcttaa gaaatctgct ggtaccggaa cacctgtcgc ctataaaaag
     3001 gctggccaac agactttaat ggccggggcg atcgataagc caaacactta tacggtcaaa
     3061 attgatgtgg aaatggaggg ttctttgaag attaaaaaag ttgataaaga atcgggtgat
     3121 attataccag gaacggtttt ccatttagac tttggaaaaa ctttacccgc aaaagacgtg
     3181 accaccgata aagaaggcat tgcgacctta gatgagattc ctcatggtac aaaggtaacc
     3241 attactgaaa aatcggtgcc ggcgccttat acgattgata ccacacccat gactgccaca
     3301 attaaagcag gtgaaaccat ttcagtgact tcaaaaaata cgcgagaaaa aggtcaaatt
     3361 attctggata aaaccggaat tgaaacaggg agtgatcttt ggaacgataa ttactcttta
     3421 gcgggcaata cgtttgccat tcgcaaggat agcccaactg gtgaaatcgt ccaagaaatg
     3481 accacggatg aaaaaggtca tgcagaaaca ccaaaagaga ttgccaatgc gttggaattg
     3541 ggaacctact atgtgactga aaccaaggct agtcatggct ttgtgaatac tttcaaacca
     3601 gtaaaagtcg aattgaatta tgtcaatcaa acagtggctc ttgttaccag taacgtaaaa
     3661 gggcaaaacc aagaaattac tggtgaaacc actttgacaa aagaagacaa agataccggt
     3721 catgaaactc aagggaaagc tgagtttaaa agagctgaat acaccttgtt tactgttaag
     3781 gatggaaaag cggtcaaatg gaccgagaac tttaaaccag aattgttgaa gggaacgaaa
     3841 gcttctgatg aaaccgtaac tttgacttta gatgaaaaga atcaagttgc cgttaaacac
     3901 ttagcgatta atgagtatta ctggcaagaa accaaagcgc ctgaaggata taccttggat
     3961 gaaacaaagt atcctgtatc catcaaaaag gtggatgata gcgaaaaaaa tgctgtgatt
     4021 actcgagatg ttgcggcaaa agaacaagtg attcgctttg gctttgattt ctttaaattt
     4081 gctggatcaa ctactggcac tgccgaaact ggcttcaatg atctaacctt taaagtgtcg
     4141 ccattggaag gaaccaatga aatcaccggt gccgaagatg aagcgaccac agcttataac
     4201 gagcaattag ggtttgatgg ttatggcaag tttgaaaatc ttccttatgg ggattattta
     4261 cttgaagaag tagaagcccc agaaggtttt caaaagatta cgccattaga aatccgttct
     4321 acttttaaag aaaataaaga ggattatacc aagagtgatt atatctttac tattaccgaa
     4381 aaagggcaaa agcaaccgat taaaacggta acggtccctt atgggaatct aaccaatgag
     4441 aatttctcag tcagtttgaa ccgtttgatg ctttatgatt tgcctgagga agaagatagc
     4501 ttaacttctc ttgcgacttg gaaagaagga gataaagaat tgactactct tgattctacc
     4561 gagctagtcg ataaattgag ctataatcta catgaaatca aagaagactg gtatatcgta
     4621 gctcaagcca tcgatgtaga tgctacaaaa gctgcccaag aaaaagataa aaaagccaaa
     4681 ccggtggcga ttgcggaaac aaccgcaact ttagcgaata aagagaaaac aggaacttgg
     4741 gagattctgc ataaattaac tgtcgaacaa gttttagaca agaccattgt cttattcaac
     4801 tatgtgtatg aaagcaaaga agcctatgaa gctggtgatg agccggtagc gaaggatgct
     4861 agcttgaaca atcaagcgca aactgttaat tgtatggtgg aacgtcatgt ttccatccaa
     4921 accaaagcgc acttagaaga aggttctcag acctttactc atggtgacgt ggtagatatg
     4981 tttgatgatg tatccattac ccatgatgta ctagatggct caaaagaagc ttttgaaaca
     5041 attctgtacg ctttatttcc agatggtaca agtaaagaaa tttggaaatc tggcaagatt
     5101 gattacgaag tgaatgacaa agaatttacc aaaaccgtac ttgcggaaaa agtggataca
     5161 agtaaatatc cagaaggtac aagttttacc ttcaaagaaa ttaactatga caaagatggg
     5221 aatatcaatg gaaaacacaa tgaagatttg aaagaaaaat ctcaaacttt aacgcccaaa
     5281 aaagttccga caacaccaag tacaccagaa caatctgaaa tgccaactgt tccaagtggc
     5341 tctcaagaat ctagtcctac agtgaaggca ttccctcaaa ccggggagaa aacttccaat
     5401 gtgctactgt tcattggctt tagcttgatc tttgcgacgg ctggctatta tttttggaat
     5461 cgtcggaact aaggtgataa gatgaaacat gtaaatagta gaaaacaata cgcgcgaccg
     5521 cctcctaaaa aagtggtcgc gctatttttg tattcaaaac taacaatgaa aagaggaaaa
     5581 aatatggaat tgaaatttgt tgtaccagat atggtcgaga cgttcggaaa actaacatac
     5641 ggtggtgaag gtgaagttct atcggaaggg tatggtcgaa atactacaat tgttggtcga
     5701 agttaccatc tgttttcaaa taaacaacgg gccgatgata ttgaagtggt ggtagctgcg
     5761 gaagccggcg aaaaggattt tgaccaagat caaccactaa aagctgtaaa tccccactta
     5821 attgccaaag gctatgaaat tgagaatcgt gggtttaccg attacgtgtt gtatgttgat
     5881 gatttagtga aagcatagga ggaaaagaag atgagattaa cagaaggtat tgtcgtagat
     5941 tcaggattaa cctttggcaa gttgcgtttt tccgcattac gtcgagaagt acgcaagcaa
     6001 aatgaggacg gaacggttag taacgaggta aaagaacgga cctataattt gaaatcctcc
     6061 gcgcaaggtc gaatgatcca agtcagtatt ccagcaaatg tacccttacg cgagtttgct
     6121 tatgatgcag aggtagagct ggtcaatcca atcgtggata cggtcgccaa ttatgttttt
     6181 cgggaaggaa ccaccgttaa ttggtttatc aaggcagatg atttagtctt gaaacgacaa
     6241 ctgaatcaag gaaaacctac gaaccaaaat gaaggaaaga aataggtgaa gaatatggaa
     6301 attaatgttg aacatatttt agattgtctc gatcaatttg gcaaaggtga gctaacggag
     6361 gaacaactca ctaacgcatt aacctatgat gaaaaaatgt ttctgattat gcatcaaggg
     6421 ttactggaag taggtaacga tgccaaagaa gactttgatg tactggattg gttaggggaa
     6481 gaagagtctt tctttatggt tgtcgaggta aatgaaagtt tatgtcggca ggcagaatct
     6541 gttttggaag aaatcgatgt ggagatgccg gatgccatcg aaagattttt aaaacaatta
     6601 gtggaaacga aacaacttcc cgtagtagta aatgactagc ataacttttt gaaattgccc
     6661 aatgaaatcc tattgggctt tttcgactgg aggtggaaac catcaaaatg tacaaaggac
     6721 atcgcatacg cgcaggagat caacacttga tttatcactt tgttcttggc tggctaatct
     6781 ttttgttcat tggttggatg agcgtctttt atttacaaga acttagacaa tttgatattt
     6841 ctaaggtatc attttccact agtgaaattg gctggtctat gaaggatgta atctgcttgc
     6901 taggaagtct cgctctttca ggaagcatga tgttacttta tatacacttt tttcaggatc
     6961 attggcgaag tctttggcat cgacaaaagt tagctcggat gattctagaa aaccattggt
     7021 atgaagtaaa gcagactcaa ggtgaaggtt ttttcaaaga tttgaatagt agttggacca
     7081 aagagagtat cagttacttc cccaaaattt attatcgaat gaaagatggc ttactttcta
     7141 ttcgcgttca aatttcactg ggaaaatatc aggatcagct attgaagttg gaaaataagt
     7201 tagaaagcgg actgtattgt gagttagtag aaaaagaact caaagactcc tatgtggaat
     7261 acaccttgtt atacgatatg attgccaatc gaattgggat agaagaaata gtggcagaaa
     7321 atggggcgtt gcgattgatg aaaaatcaag tatgggcgta tgattccttg ccgcatatgt
     7381 taattgctgg cggcacaggt ggtgggaaga cctatttctt actcaccatt attcaagcac
     7441 tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa aaatgcggac ctcgccgatt
     7501 taggtacgat aatgcctcac gtatactccc aaaaagaaga aatttctgaa tgtgtggaag
     7561 atttttacga acgcatgata gctcgtagta ggtcaatgaa agaaatgccc aactacaaaa
     7621 caggagagaa ttatgcgtat ctcggacttc cgcccaactt tttaatcttt gatgaatacg
     7681 tggcttatat gggtgcgaat cgttttccta caagcatcga atagatgtga aatggaagcc
     7741 tattgagatt atcaatagtg acaactaatg ttttgagaga tggaatgaaa gccttcaagt
     7801 tgagctgaaa catccccgct aatactccta catgctctaa tcaatgaagt agattggttt
     7861 tggtatgaag ctaggtgaag tcgtctgaaa tctgcctaag tgcttgagca taaggctgac
     7921 tatagggcgg gcggctgaaa aagataacta tggtaagaat gtctgaaaat gactgacgaa
     7981 tctgcgaatg tacgggtcta aaaggctgat cttattgaaa gataggaaca tacggacgta
     8041 tggtgagata ccgacaagta aggttccatg atatgaaaat cgggatagct aacgaagaag
     8101 ctataatttc acaggctcat agtagatacc taaggttata catgatagct aaaaacattg
     8161 gaacgatgta agcagaaaac tatcaattaa atgactactt ctagataggt gcatataagt
     8221 aataacgaaa ttgctttgtt tctgtgaaag taagggcaca gtaccgatga agcgtctaac
     8281 aaatgtggag ggatagccct aagtctattt aataacaatg aatgttgaaa cagatgaagt
     8341 tcaaggatct ggtaagaacg tgggaaaatc cgttatggag gtgccacgct tgaacacaaa
     8401 aatacgttac tgggaatatt atgatctaca agaaacattc gatcagctat ttactcaaag
     8461 tagaaacggg aaaaagtttt atcaattata tgagttaatc atttctgaaa ataatattct
     8521 tttagcatac cgtactatca aggcaaacaa ggggtcttcg acaccaggaa cggattcctt
     8581 cacaattgat aactataagg aaatgaacca agcagagttt atccatctta ttctcagtca
     8641 tttagaaaat tacaaaccaa aatccataaa gcgagtaatg atacctaaac ccaatggtga
     8701 gaaacgccca ttaggaattc catgcatgat tgatcgaatg atccaacaga tgttcaagca
     8761 gatactggag ccaatatgtg aagctaagtt ttatgaacat agctacggtt tcagaccatt
     8821 gagaagtgca aagcatgctc taggtcgcat tatgtatctc ataaacatca gtaaaatgca
     8881 ctatgcagta gacattgata tcaaaggttt ctttgataat gtaaaccacc gtttactgat
     8941 aaaacaatta tggaatatag gaatttgtga caaacaagtc ttagctattc tttcaaaatc
     9001 tctgaaatca cccattcaag gagaaggtat cccgagcaaa ggaacgatcc aaggaggtat
     9061 catttctcca ctattatcta atgttgttct aaacgactta gaccattggg tctcaaagca
     9121 gtggcatacc tttgaaacaa agtatcccta tactaaaggt tacaataaat ttcgtgctct
     9181 aagagataca aatctaaagc aaggctatat tgtcaggtac gcagatgact ttaaaatcat
     9241 gactaatgat tatccgacag cattaaaatg gttccacgct gtcaagcttt acctaaaaga
     9301 cagacttaaa ttagatattt ccaatgagaa gtctaaaatc gtcaatttgc gtaaatgtaa
     9361 gtcggaattt ttaggattcg ctatttgcgt gaaacaaaaa gggaaaaaat gggtttgtaa
     9421 ttcacgtatt tctaacaaaa aaaaggatca gattaaagaa gagattaaac aaagaatcaa
     9481 agatattcaa aagagtccta cagcacaaaa tgctctcctg tttaatgctc tcgtgctcgg
     9541 tgttcataat tatttcaggt atgccactca tattaattta gatttgaata ggatgaacta
     9601 cgttctttca ttcactactt acaaccgatt atcaaattgt agtacaagat gttaccccaa
     9661 caaagcttcc ccaacttaca aaaagtttta ctcaacaacg atgaaaacgt ataaagtagc
     9721 tggagtctat ttgtaccctc tttgcgacgt atctacaaaa acaatttacg gatttaatac
     9781 ggaggataca ccgtttactc cgtttggtcg tcaaagattg gagcacaaaa gcctacagtc
     9841 tcttgtgttc caagaattaa gaaaattgat ggaatcgaaa ataccgaata gaacggttga
     9901 gtatttagat agtagaatat ctcgttactc aatgaaatca ggcaagtgtg agattacaaa
     9961 acaatttttg ccagcaaaag ccgttcactg tcatcactat ttgccaaaaa gtcttggtgg
    10021 agacgacaaa ttcgataact tgagaattat tcataaggat atccatcttc tgatccatac
    10081 aacaaataaa atgataatcg atcactacgt gcatgaactg aaactggtgc cagagcaaat
    10141 agcaaaaatc aacctttatc gaaagatgtg taatctgcaa aacattcaat agaaaataaa
    10201 tagatggaac gccgtatgag gtgaaagtct cacgtacggt gtggagcggg ggaaaagatg
    10261 gagattatat caaattctta cctatcgcta tagatgttaa cgacaaaaga aagtgcggtg
    10321 attttgaata agcttaaaca aatcgtgatg ttaggccgtc aatctggttt ctttctgatt
    10381 ctagcttgtc aaaggccgga tgcgaaatat ctaggagatg ggattcgcga tcagtttaac
    10441 ttccgtgtgg ctttgggtcg tatgagtgag cttgggtatt ccatgatgtt tggagaagtc
    10501 gataaaaatt tttttatgaa acgcatcaaa ggacgaggtt atgtagatac agggggcagt
    10561 gtgattagtg agttttatac gccacttgta ccaaaagggt atgatttctt aagggaaatt
    10621 agcaacattg ttggattaac cgttcatacg gaaagagaaa ataatagtgt gtagcctagg
    10681 aatatcaata atcctgggct tttttattga ggtgatttat gacattttta gatttattcg
    10741 ccgggattgg tggctttcgc ttaggtatgg aacaagccgg tcaccagtgt atcggttttt
    10801 gtgaaattga tgaatttgct cggaggagtt ataaagcaat ccatgataca aggaaggagg
    10861 tggaaatgca tgacatcaca agtgtatcag acgaatttgt tcgatccctc ggaccagtgg
    10921 acatcctttg cggcggcttt ccgtgccaag ctttttcaat tgcgggaaag cggcaaggat
    10981 tttccgatac tcgaggtact ttattctttg aaatcgctcg gttcgccact cttctccaac
    11041 ctaagtttct attccttgag aacgtccggg gattgctcaa tcacgaagga ggggctacgt
    11101 tcgagacgat cctccgaacg ttggatggat tggggtatga tgtggaatgg caagtgctta
    11161 actcaaaggc ctacgttccc caaagccgtg agcggatctt ccttatcgga cattctcgag
    11221 atgcatgtac cgaacaagta tttcctatca ttggatcgtc ttcaacatct gatcaaaaca
    11281 tcagaaactt ggtaaatatc aatccttcca atcgaggaat gggtggccaa gtctatggtt
    11341 cagatagcgt agcaccaacc ttgaccggtg atgaaggaat taaaattgcc ttaccggtaa
    11401 aggatgggat ttctgtagcg ggtatgttgt cgggaaattt tgaacaaggc aataggattt
    11461 atgaggtaac cggaacagcc cccactttgt caacgaaaca agggggaacg aagattatga
    11521 ttcggaaaaa tgaaacagaa tatcaagagg tgaagccagg ggacagtgtg aacctggctt
    11581 ttcctaattc cacaagtcgc cgaggtcgtc tgggaaaaca aagtgtgcat accttactaa
    11641 caggagacca acaagcggta gtcacggatc agtaccagat tcgaaagtta acacctaggg
    11701 agtgctggcg gcttcagggc tttccagatt gggcttttga tcgagcttct caagtcaatt
    11761 cagatagtca gttgtataaa caggccggaa actcagtaac agttccggtc atttttgata
    11821 ttgctagaag attaaagggg gtaaaagcat atgatttatg acgagttgat tggagaaatc
    11881 tactggatga ttggcaagat tcaggcagat ccgaaattgg aagaagaact gtaccggctc
    11941 aattttgata ttcgcaaaaa cggggtgaaa gtccaggtga ttcgtatgta gtggatgagg
    12001 aaacggatgc tcgaatggag ctcaatcaag tcatcggtga attggaacga attgcggacc
    12061 ttgccaaaga acctgatatc cgccaatact tgtttgagat aaaagcagag ttggaaattg
    12121 agggtatcac cgcggagtaa tagggaaata acccactgat aggcactgac cgcgggggat
    12181 ttgcgaagca gaaatcctcc gtggtgctgt gcctgttcat ggacggcggc agccggccat
    12241 gatgtctcac cccccgtatc taacaggggg gtacaaaaac acaaaaaaca agcaagtaac
    12301 cataaaaaat gcttgttgta tcaaggatat taagtaattt gtataggtgt taactcattt
    12361 ggaaaagtta acaccttgaa tttggaggtg aaaacttggc gcaacgaaat ttagattatc
    12421 ggttattaaa agaacgacgc aacgaatatg gcgtttcaca aaataaatta gctactgctt
    12481 gcggacttag ccgcccatac ctaaatcaga tagaaaacgg tggcgtaatc gcctctacaa
    12541 agaccatgag aaaaattttt gatcaactgg aaagtttcaa tcccgattta cctttaacgt
    12601 tactatttga ctatgtaagg attcgctttc ccacaacgga tgtgcggaaa attattcaag
    12661 aaattctcca tttgaaattc gagtatatgc ttcatgaaga ttacgccttt tactcctatc
    12721 aagaacaata tgtcatgggc gatattgtag tgatgttatc tcatgaagaa gataaaggtg
    12781 tccttttgga attaaaaggt cgtggttgcc ggcagtttga aactttttta ctcgcccaaa
    12841 agcgtagctg gtacgacttt tttgaagatt gtctaaaagc cggtggcgtg atgaaacgct
    12901 tggatttagc aatcaatgat cgagtggggc tactggatat tcctgattta acgaaaaaat
    12961 gtcagaagga agaatgtatc tccttgtttc gtaccttcaa aagttaccgt tccggggaac
    13021 tgttgaaagc tgatgaaaag gatggtatgg gcaatacctt atatattggc agcctcaaaa
    13081 gcgaggttta tttttgtttg tacgaaaaag attatgaaca atatatcaag ttagggattc
    13141 cattggacca gaccgaaacg aaaaatcgtt ttgagattcg gttgaaaaat gaccgtgctt
    13201 attatgcgat tcaagatttg ttaaaaggtc gtagcattga aagtaccacg ttttccatta
    13261 tcaatcggta tttgcgattt gcggataaag tagatggaaa acggcgaacc aattggccct
    13321 tgaatgaaca atggggtcgt tttattggac ggaatcggaa agagattcaa ttaacttctg
    13381 aaccgaaacc gtataccatt gaacgaacat taaattggct aggtagacaa gtcgccccta
    13441 cgtggaaaat ggctaaggag ttggatcgat tgaatcaaac aacgtatatc caggatatgg
    13501 tacagaatgc gcgactctcg gaccggcata aaaagatttt ggagcaacaa agtatggcaa
    13561 tcgaaaactt gattgtatga aaagaggaat tttgatggaa attgtaaaag atatgttatt
    13621 ggtacttggt ggcagtatgc ttggtgttac cgtgatgtgc ttgatgaatg caagtgcagc
    13681 agcggattga gcaatggaaa agaaaggaaa gtgacaaaat gaatttcgga caaaattt
//
LOCUS       DAYYAO010000069        13720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000069 DAYYAO010000000
VERSION     DAYYAO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.072"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..1546
                     /locus_tag="ACHARI_002040"
     CDS             119..1546
                     /locus_tag="ACHARI_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443065.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1638..2003)
                     /locus_tag="ACHARI_002041"
     CDS             complement(1638..2003)
                     /locus_tag="ACHARI_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8443066.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2000..2365)
                     /locus_tag="ACHARI_002042"
     CDS             complement(2000..2365)
                     /locus_tag="ACHARI_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8443067.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2557..2805)
                     /locus_tag="ACHARI_002043"
     CDS             complement(2557..2805)
                     /locus_tag="ACHARI_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443068.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2804..2932
                     /locus_tag="ACHARI_002044"
     CDS             2804..2932
                     /locus_tag="ACHARI_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8443069.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2989..4683
                     /locus_tag="ACHARI_002045"
     CDS             2989..4683
                     /locus_tag="ACHARI_002045"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8443070.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4783..5079
                     /locus_tag="ACHARI_002046"
     CDS             4783..5079
                     /locus_tag="ACHARI_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443071.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5457..6590
                     /locus_tag="ACHARI_002047"
     CDS             5457..6590
                     /locus_tag="ACHARI_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8443072.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6621..6941
                     /locus_tag="ACHARI_002048"
     CDS             6621..6941
                     /locus_tag="ACHARI_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8443073.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6970..7434)
                     /locus_tag="ACHARI_002049"
     CDS             complement(6970..7434)
                     /locus_tag="ACHARI_002049"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8443074.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7565..8818)
                     /locus_tag="ACHARI_002050"
     CDS             complement(7565..8818)
                     /locus_tag="ACHARI_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8443075.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8847..9164)
                     /locus_tag="ACHARI_002051"
     CDS             complement(8847..9164)
                     /locus_tag="ACHARI_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8443076.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9190..9510)
                     /locus_tag="ACHARI_002052"
     CDS             complement(9190..9510)
                     /locus_tag="ACHARI_002052"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8443077.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9974..11389)
                     /locus_tag="ACHARI_002053"
     CDS             complement(9974..11389)
                     /locus_tag="ACHARI_002053"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8443078.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11406..12722)
                     /locus_tag="ACHARI_002054"
     CDS             complement(11406..12722)
                     /locus_tag="ACHARI_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8443079.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12872..13570)
                     /locus_tag="ACHARI_002055"
     CDS             complement(12872..13570)
                     /locus_tag="ACHARI_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8443080.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4359 a         2785 c         2359 g         4217 t
ORIGIN      
        1 aaaacctttt ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag
       61 aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag gctctactat
      121 gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg ggcaattcta
      181 tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac
      241 tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca
      301 tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat
      361 gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga
      421 tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc
      481 attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg accgctactc
      541 ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg
      601 cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga
      661 atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat
      721 tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt
      781 tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac
      841 tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac tattcaatct
      901 ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca
      961 agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt
     1021 caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc
     1081 ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca
     1141 gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta
     1201 tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct
     1261 ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct
     1321 gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat
     1381 ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg
     1441 tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat
     1501 caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa
     1561 aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac
     1621 acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc tttatttaaa
     1681 aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag
     1741 gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt
     1801 atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc gctttgcact
     1861 tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc
     1921 aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga
     1981 aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta
     2041 tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca
     2101 aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt
     2161 ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac
     2221 gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga
     2281 tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa
     2341 attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt
     2401 cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg
     2461 gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg
     2521 aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa
     2581 gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc
     2641 ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc
     2701 ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga
     2761 actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat
     2821 caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac
     2881 aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat
     2941 ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt
     3001 aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa
     3061 gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat
     3121 gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa
     3181 aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat
     3241 ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa
     3301 gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt
     3361 ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg
     3421 actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa
     3481 gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca
     3541 acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg
     3601 atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa
     3661 tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt
     3721 ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact
     3781 gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat
     3841 gaagatacag cagttatttt cactaatggg tccattacag attcagctac tttaggagat
     3901 tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa
     3961 gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca
     4021 agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc
     4081 cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc
     4141 acgccgttag gccaaaaaga tgtcaatatg tctatcgtcg tgcatcatca accgcatttt
     4201 acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt
     4261 ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg
     4321 attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa
     4381 attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc
     4441 aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc
     4501 tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta
     4561 cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa
     4621 acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa
     4681 taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa
     4741 cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc ttttgaacac
     4801 cgtttaccaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac acccaagcta
     4861 aaactgcttc ttatgttttt gacttggttg gacttattac cgcagatgca agcttttgaa
     4921 caccatttac caagaggtta tgaaaaagct gccctgcatc aagtaataag agcagccaag
     4981 ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat gcaagctttt
     5041 gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt
     5101 ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag aaactgatcc
     5161 cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac tattttttca
     5221 acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc aatgattacc
     5281 attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga ctatagaatg
     5341 tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt taaaatgaaa
     5401 ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca tctccaatga
     5461 aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa
     5521 tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc ggctccgatc
     5581 tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggacacgaac
     5641 caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg
     5701 tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat cacatggaaa
     5761 gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga tttggcaagt
     5821 tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat gcagactcta
     5881 cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt ctatcagata
     5941 ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg
     6001 tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt atggctggag
     6061 cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg gctaattaca
     6121 atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa ttgtatgaac
     6181 tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga ttagagcctg
     6241 tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct ccattacaga
     6301 tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt tatatgactc
     6361 cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc aagcatggac
     6421 aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa ggactattcg
     6481 atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa gcatatgaga
     6541 tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga attcagtaaa
     6601 aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag
     6661 gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaatcgtt
     6721 acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt tttggaagcc
     6781 ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgcta
     6841 ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt tacgacgaaa
     6901 aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc aaatctcatg
     6961 cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc cactttcttc
     7021 cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat gatctgtaat
     7081 taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg ctaatatccg
     7141 cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa gatttggtgt
     7201 cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga cgatggtttc
     7261 tttttcatct aagaccacat accattgagg aatactttcc ttattagcga ttccagtacg
     7321 aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc aattcgcgct
     7381 ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa ccaatgttta
     7441 aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga aaattagcaa
     7501 tgacaacaat agtgatgtaa aaaaaaaacg gaaaacaatc acttgttctc cgttttttga
     7561 ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc ttgacgaacg
     7621 gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca cctgcaatac
     7681 tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc ataacggtag
     7741 ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag accattggtg
     7801 tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca aacatcgttg
     7861 gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc atatatggtt
     7921 tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca cctgttcctc
     7981 ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg ctatgcccag
     8041 ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc atcaaaggat
     8101 cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata aatgcaagca
     8161 gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc actgtataga
     8221 aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact aaagcaaacg
     8281 cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact gccggtggca
     8341 caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca aagatttctg
     8401 ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata cctaatgctc
     8461 cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg tcttttcggt
     8521 agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga atggcaaatg
     8581 taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa attgagttca
     8641 tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat gctcctaaga
     8701 tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc cgttgtgcac
     8761 caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat ttatccataa
     8821 atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag atcaatgatc
     8881 tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact gtggaccgtc
     8941 aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc tgtttgagag
     9001 tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc taaagtgaaa
     9061 tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt tccgccaaac
     9121 atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg tatcctccga
     9181 taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt caccattcat
     9241 catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg ctagcttttc
     9301 agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt ctacattttt
     9361 agattctagt tgattatccg catctgaagc agatacagca aagatgtctg cttccattcc
     9421 ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca ttccagctga
     9481 acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta tacttacact
     9541 tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga tatcatcaca
     9601 tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact aaacacacaa
     9661 attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt
     9721 gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta ataagtccaa
     9781 ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt gatgcaggac
     9841 agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg tattagctca
     9901 aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat gaacatctac
     9961 aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc ttatcaatca
    10021 aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg ttcaattcga
    10081 ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag tcagtgaatg
    10141 cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc aagtaatgca
    10201 aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc tcatccatat
    10261 actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg atattatgat
    10321 aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt tcccatcccc
    10381 tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt tcaggatgga
    10441 aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga taatattgat
    10501 tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct gttgcaggat
    10561 cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag tatcctttta
    10621 ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca gctttttggt
    10681 cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag ccaatccttc
    10741 caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa actttatgat
    10801 aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat
    10861 agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt accctatcac
    10921 cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact tttcttgaag
    10981 accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc aaacaaatca
    11041 tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc tgagcatatt
    11101 cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg atagaagtcc
    11161 gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat tgttccataa
    11221 aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca tttttatacc
    11281 agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc gtttgccaag
    11341 cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg atttcctcct
    11401 gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg cagatagatc
    11461 aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt tcctgctgtt
    11521 gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac tgctttcgcc
    11581 gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg cgttaagata
    11641 aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac tggttcattg
    11701 atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc ttcccgtttt
    11761 ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc catactcata
    11821 tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc ttcaaaagct
    11881 tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt caccatttga
    11941 tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag caaaataccc
    12001 ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat gatatcataa
    12061 aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc cgatacagtc
    12121 agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac ggtatcaggc
    12181 attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat agaaaggatg
    12241 gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc caatacgttt
    12301 tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt aacagataaa
    12361 taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag cttggctaat
    12421 tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac atagctgatg
    12481 gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg tgctaagcca
    12541 ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt aataattacc
    12601 ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt attgattctc
    12661 gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc agatcccctc
    12721 attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg cttacattta
    12781 gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa ttcatctgat
    12841 gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct cctattattt
    12901 tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac gcagcaagtg
    12961 cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc tcgattttca
    13021 gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg attgggataa
    13081 tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta
    13141 attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc gttaattttt
    13201 tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc atcttaggct
    13261 ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct tcaatcaaag
    13321 aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc cccgctcctt
    13381 gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc accgtgcttc
    13441 gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct cctacttgca
    13501 actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact agggttttat
    13561 tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat tattataaca
    13621 cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc atagacatac
    13681 aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
//
LOCUS       DAYYAO010000070        13627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000070 DAYYAO010000000
VERSION     DAYYAO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.073"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..941)
                     /locus_tag="ACHARI_002056"
                     /pseudo
     CDS             complement(243..941)
                     /locus_tag="ACHARI_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            1020..1196
                     /locus_tag="ACHARI_002057"
                     /pseudo
     CDS             1020..1196
                     /locus_tag="ACHARI_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1372..1941)
                     /locus_tag="ACHARI_002058"
     CDS             complement(1372..1941)
                     /locus_tag="ACHARI_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8443081.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2015..2338)
                     /locus_tag="ACHARI_002059"
     CDS             complement(2015..2338)
                     /locus_tag="ACHARI_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443082.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2492..3304)
                     /locus_tag="ACHARI_002060"
     CDS             complement(2492..3304)
                     /locus_tag="ACHARI_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443083.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3631..4506
                     /locus_tag="ACHARI_002061"
     CDS             3631..4506
                     /locus_tag="ACHARI_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8443084.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4520..4843
                     /locus_tag="ACHARI_002062"
     CDS             4520..4843
                     /locus_tag="ACHARI_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443085.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4840..5592
                     /locus_tag="ACHARI_002063"
     CDS             4840..5592
                     /locus_tag="ACHARI_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8443086.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5582..7753
                     /locus_tag="ACHARI_002064"
                     /pseudo
     CDS             5582..7753
                     /locus_tag="ACHARI_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7865..10036)
                     /gene="copB"
                     /locus_tag="ACHARI_002065"
     CDS             complement(7865..10036)
                     /gene="copB"
                     /locus_tag="ACHARI_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8443087.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10056..12242)
                     /locus_tag="ACHARI_002066"
     CDS             complement(10056..12242)
                     /locus_tag="ACHARI_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8443088.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12242..12451)
                     /gene="copZ"
                     /locus_tag="ACHARI_002067"
     CDS             complement(12242..12451)
                     /gene="copZ"
                     /locus_tag="ACHARI_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8443089.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12464..12904)
                     /locus_tag="ACHARI_002068"
     CDS             complement(12464..12904)
                     /locus_tag="ACHARI_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8443090.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12978..13517)
                     /locus_tag="ACHARI_002069"
     CDS             complement(12978..13517)
                     /locus_tag="ACHARI_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8443091.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3836 a         2844 c         2418 g         4529 t
ORIGIN      
        1 tgtcaatttg tgtacatcct aaaatataag ctatttgttt acttctgtag aatatatact
       61 ttgttgttag ataagttgca catgtttata gaatctaaat aataaatttt tatgtaccgc
      121 ccctcaaagt gaactgaacc ccaaaagttg aactatttaa tggactgttt ccgatattct
      181 actggagata gtccattaaa tagttcaact tttggggttc agttcaaaga gggggggatt
      241 ttttatcgtt gtggaatatt taaataccaa cgcttgtcat gaaaatcttg tgctccaccc
      301 ttagtatttc cttctggatc gtttgtcgca cgcatcatga cgtatacttt cttattaggg
      361 aaatcacgca tattgaaaga tacatgatag ccaacatttc ctaaagtatt ataagcttga
      421 tttacgtctg gtctataaat cccatcagct cttactctag ctagttcttt tccagtattg
      481 tagtccataa tgaaaatata ctcgtattta taattagcaa tgtgccatcc agctacatgc
      541 aagtttgcgt tttcgatttc tccgaactga tcaatgtggg cgtaatttgt tccatctgtc
      601 agcgtagaat ttgcagcacc tgctcgagtt ggatcaatta ctggtttatc ctccgaagta
      661 gttggattat catccgtaaa accatgagcc aaatcatagg ctagtttttc tttacttaca
      721 cccatttcag aaagataacc gtaaggatct gtatgatcgc cccaaatatt ttgtgttacc
      781 cataaatgcg atttgattcc tggttggtta taaggagtgt ctaatgttaa tggaatacca
      841 tatttcattg ctgaatctct tgccaattca acgtatactt tatagttctt ttcaaaagtt
      901 tctttatcat gtgtatgttg taactcgatt tgcacaggac ttttggctca cgaggttgtc
      961 ctactctaca tcttgctgat agatatgctg aaaggaaaac accaacatta tgttttgctt
     1021 tttcagctag aacgagacaa gcaggaatac ctttagaaga tatcaaaggt tttttaggtc
     1081 ataaagatgt ttccactaca caagtctatg cccatatttc tcccgaagtg aaaaaaagat
     1141 caatgaatca actagaaaac tatatagaag agcaaataaa aaagcactca aattgagtgc
     1201 ttttttaagc tcactatcac taaaactatc actaatttta tttataagcg aacatgtttc
     1261 tatcactgat tttttaaatc tagcgaaaaa cccttatctt aaaggcgttc gttcaattct
     1321 ttggcaaggt cttcaaagcc tggcttgcct agaagtgcga acatggtttt cttatagctc
     1381 atgtagaaaa attttcttga tgatgatttt atccatcaat tgagattgac tgttttgctc
     1441 tcctatgatt tcagctatga ttgtccgatt ttgcggtttt cttcttaact ggtagttgat
     1501 tttcccagtt tccatcaact cgctaagcat atccacgact ccgccaaatc cggtcttttt
     1561 cgtaataaaa cctagtgcta ccattcgttc atctagattt tttgctgtaa tcgtcacacc
     1621 agtaatgagc gagtgctgta atgtttgggc cagctcttcc atcccttcag cattggatac
     1681 gacggaagct gttttgttaa taggttcttc gtttatttct tttggttctt tcctctccac
     1741 tttcactgtt tgacgaagga catctagttc ttgcaagagc gtttcgtatt gttcttgggt
     1801 cagcatcacg ccagctactt tttcacgatt aaatatatat acgccagttc ccgcgtctcg
     1861 cgcttgagca aatacatcca tcggcgatcg tttcacttct gtgatcgaag atgtgggaac
     1921 ttctaatttc tttaattcca atggatttcc tcctttacta atcgcttttt attatagcac
     1981 agactgactt gttttgatct tttttccaaa ggttttatcg gtttttgcct aaccagtaat
     2041 tgatatcggt aagaagtctt tcgataccaa atgaaggatg gtacagatac gttttttgat
     2101 cttcgttatg gtctagtgga aaaaaatgat cgatgatcac tagatctgca taccgaggcg
     2161 gcttatttcg agtacataca tgtatagaga tccgttttcg atgtcttagc gttttgttca
     2221 gtaccccagc aagtcttctt gcttcttggt gttcatagcg aaaaaaaaag acgattttca
     2281 cttcaggagg gtggacatct aaataccctc gttcctggag tgttttcagt aaaagcatcc
     2341 cccactctac tgcggggtgt gcaagtgctt gttggatcgc tagccgaaat tgatatagtt
     2401 tatctttgga attgggaaat ttttctaatg tttcctgata ataacgaact tcttctatca
     2461 gccggtcatg gataccaatt acgtaacgaa gtcattcttg gtaaagtact tttgtcagct
     2521 ttgatttgct ttcgctctca aattcgctat tcagccaaaa cagcgaatca agcttttcag
     2581 tcacaatcgt caatttccga tagtcttgtg tctcttctat ccgtttccac cgcttttcag
     2641 taatctggtt acagctcaag cgcaaaattg ctagataatt tttttctttt attggcaaaa
     2701 tctgctcctc tctaaatagc aattcaggaa acaattcaaa gaatagtgtt cgactgtctc
     2761 ctaaaatctc gatttctgcc ggatcctttc tttgcttcaa taattggatg aacatctcag
     2821 aaaaggactg ctctccatca tctgcaaaaa aatgcggaaa atctttcaaa aagcaaaaca
     2881 gcagatatct tacttttctt tctgaaccaa agatctgtgc ttttttataa gaaaaagaaa
     2941 ggtggtactc gccaaaccac tttctcaatc gctcttgcgt agaacgatcg tacttctgct
     3001 cgtaaggttc attcaatagc agatttttca gtaaacaaaa cgggggtgat tggatcagaa
     3061 aaacaaacaa gagataaggt tcgctatatc cttgatcata aactaactga acggttttct
     3121 tatgaagaat cagctgaatg ggacagtcgc tttccgccaa acgtttcaga tgccgatcca
     3181 ctgttttaac atcgattttc agttcttcag ctaaatcagc ttttcgccag atcaaatctt
     3241 tcattagatt gattagtgaa aataaattct gtgcattttt tgataatagt ttttgccatc
     3301 tcatcgatca tttctcacct gatttctaaa catattgttt gatcaaaatg ggttcactcc
     3361 taaaaagata aaaaactccc tttctattta tttaaatacg caaatcttcc tcattatttt
     3421 ttattttact caatttaatc gtattctcat ttcaaaacga atgtacttta aaaaaacata
     3481 ataagatcac tttgcttgta ggatgtattc ccttccatta caatgaaata aatagcgatt
     3541 ttatgagaag gtgatgtgaa aatggaagaa aaaatagaag aatccaaaca aaaaatcgat
     3601 cagttgatat aagaattaga aaaagcggtc atcggcaaac gttcaactat ccaattgatg
     3661 atcgtggccc tattagcagg tggtcatgtc ttattcgaag atattcccgg tgtaggtaaa
     3721 acgttactgg tcaaagcact agcaaaagct gtacagggaa ccttcagcag agtccaatgt
     3781 actcctgatc tgctgcctag tgatattctt ggtttttccg tatacaattc acaatcaaaa
     3841 gagtttgaat ttcgtccggg accgatcttt accacgatct tgttagcaga tgagatcaat
     3901 cgtacgactc ctcgtactca gtcagcactt ttagaagcaa tggccgagaa tcatgcaacg
     3961 atcgacaaca atacataccc tctagacaat catttttttg tcttagctac gcaaaatcca
     4021 atcgagtacg aaggaacata tcctttgccg gaagcacagc tcgatcgttt tttattccga
     4081 ctacaaatcg gttatccttc ttttgatgac gaacttttgc tacttttgga taaattcgaa
     4141 aaagtgttgg ataacttaaa tgaagtattg aacagttatg aaatagaaga aataaaaaca
     4201 aatgtggcga atgtctttgt cagtcccgaa gtggcatctt atgccttgca gttagtgtca
     4261 gcaacaagaa gtcatgctgc catccagctt ggcatcagtc cacgaggaag cctttccttt
     4321 attcaggcag caaaagctta tgctttaatc catgggagaa attacgtcac gcccaaagat
     4381 ttgcaggatt tagttcctta cgtgttttcc catcgcctga ttttttacga tcgttcattg
     4441 aaggaagacg aaaagaaaca aatcattcat actcttgttg cacaaattcc tattcctgtg
     4501 aggtagattg aggtagacaa tggaaaaaat caaagattat ttcattaatt ttgtgttatt
     4561 tctggtatat ttcatcattg tgctttttgc tcttgtattc aacaattctt taggctggtt
     4621 cttgttggcc ttttttactc tttatgtatc atggagcttg atttcattaa gcagctttct
     4681 aaagaaactt tcggttactt gtacattgca gccactcgtt tatcgcaaaa aaagtatgca
     4741 ggttattttt tccatagcaa aaaagagaac tttttcttta cctctccctc gtttacaaat
     4801 cggcttccca gcaatctttg cagaaaaaaa caagagattt tgattcttac aaataaaccg
     4861 caagaagtcc aattgctttg gcagccaaaa gaaagaggat gccatgaaac attaactgtc
     4921 atttttacag cctatgatag tctaggactt ttcagaaaaa gtgcaaaaag agagatctct
     4981 tttcctgtga ctgttctgcc tgctttttgt aagattcaag cagaaaaatt acagagagtt
     5041 ctggaaaaaa aacagcagct gaacgcgtac caaagaggtc ttgattttcg ggaacatcgc
     5101 gcttatcgtc caggtgattc tttcaaccga atcgattgga aattgtctgc tcatactcaa
     5161 gagtggttat accgtgaata cgattatcaa gaagaagaat gttctcctct tttttgcttt
     5221 tggggttcgc ccagtccaaa atttgaacct ctgctagact tgtatttttc tttatggcat
     5281 ttgagaaaag aaaagcagcc ggaacttctg atattaggca accgcgcctt tcatggcaaa
     5341 gaccccggtc atacctattt tgcttctttg gaagctggcg atgagaaagc ttttttcgca
     5401 cacttgaaaa aatatgcaaa aaaacaaatc gtcctctttg ttcctgagaa ttctcctgaa
     5461 gtctcagaag ctattgaaac attgtcaaaa gatcgaacgg tttatcttgt ttatttcgta
     5521 gaaaaacaac tcatggtcca agaaaaagac aagatatgtt cattgccagg aggtgaatgg
     5581 attgacgatt aaacgggaaa aatggctgcg tggcgtttac tcctttgtga tactaagtgt
     5641 aggtgctccg atatttgtag tagctaatca attgcaaaac agctaccttc ttatcctaac
     5701 tattggtgtc ctatgcctta ttgtctgctg tattcctaaa aaatgggtac gtttgctgct
     5761 ttctttgcca gtaatgatag gttgtttgtt tttatatttt ccttcgaaga cattttccat
     5821 attatggatc ttacagtttt tttcgtcagt cacagaggaa ttcttccatc tatcccaagg
     5881 taaactttat tatttgccag aaaagacagc tttttttctc ataatgttgc tgatctattt
     5941 atttattaca ttgaccgtgg attacgacta ttggtacgca ccctttcttt ctttgatggc
     6001 ttacttcatg atgcttaccg tttttcggaa aaaagattta ctgtacgaaa tgattgcggt
     6061 agtaactgtt ttgttcgtgt atctggcagc aaggcaattt ttctccatcc ggttgttgtc
     6121 gggaaacact cgattccagt cattgactac agttctgctt atttgtttct taacttttag
     6181 cgctatcttg cctttatatc ttccgaaagt acaccaatct ctcgaggaaa cgagtgaacc
     6241 gatccgagaa tatcttaatg atgaagggct ttatgacaca ctccatgagt atcaattaac
     6301 cggaagtgca agaacaggct tcagtgagaa tgatgaacag cttggtggac ctttatatga
     6361 taatggcgaa gttgttttta cagcgaacca aaaaggaaat ggctattgga agatcgaaaa
     6421 taaaaatcat tacaccggtt ctggatggat agaagatcag gacgcaccca tccaaccaat
     6481 ccaagaattc cctgttgaca tcacgagcta ccaaaccgtt atagaagaca aagaaaacat
     6541 tacattgacc ctaaaaaaac aagttttctt accttatcct tatggaaata ctaccttgac
     6601 tgatttttca ttagctagtg gacaaacgct ttactatttg cctgaaagcc agcgattcat
     6661 tcctgtcccg aatgaatcga cacgttctgc aactttacaa acagaaaggt tagtttttac
     6721 gagagaagaa ttaaaaagca gtcagttaaa agctgatgat ttagaaaatc agcagacaaa
     6781 tcttcaattg ccaaaaagtt tgcctaaacg tattgaggac ttggcggtcg aattgaccaa
     6841 aaatgaagca actttaattc gtaaagtcga agctgtctaa cagtatttaa aaacaagcgg
     6901 aaatttccgc tattccaaaa cggataccgg gcatactcca aaagaacacg attatgtgga
     6961 ttatttcctc tttgattcaa aagtcggcta ttgtgacaac ttttcaactt ctatggtcgt
     7021 ccttcttcga acacttggta tcccagctcg ctggacaaaa ggttttacaa atgggctcag
     7081 aacaggcaca gataatcaag ggaatgagga atacagcatt ctcaatagtc atgctcattc
     7141 ttggccagag gtatactttc ctggatttgg ctggcttcca tttgaaccaa ccccttcatt
     7201 tgccaatcca atgggttcac aaccagttgc tgaaacaacc gaaaccgtcc agcctgctat
     7261 cgaacaacca aatgaaagcg aaaatatggc agaaactatt tccagtgaga gatctgaggt
     7321 cattccatca gaagaaacaa cactcacacc agcttataaa atcgtcttat tcagccttgg
     7381 agtgtgttta tccgtcttgt tgttctttct attgttattc agaaaatctc tgcgctggac
     7441 aagttatctt atctatcttc atttttttgc ttctactaat acgattgcat atcaaaaact
     7501 tcttaaccct ttggaaacat tggtaccgcg caaacccgca gaatctttgc aggaatatag
     7561 cctgcgaata gaacaagaat tcaagctaga tctgcgtttc attcgattaa ctactcaata
     7621 tgaagcagaa atatatggac gacaagcaga tccggtaaaa ttcgataaaa aaacctattt
     7681 agaaataacg catataataa gaaaaatggt atacaaaaaa attctgacaa aagggagccg
     7741 cttcaaaaaa taagataaaa tttctggaga atctacttct ggaatgccga gaaccagctg
     7801 attgagtatc gattactcga aaaaaaggtt gtgacacgtt ttttgtcaca accttttttc
     7861 attattatct gattttcaat gtcaaagcat ttagagctac aactaccgta cttaacgaca
     7921 tcaagacagc accaactgct ggacttaata tgataccaat tggcgcaaga attccggctg
     7981 ctagcggaat tgcaagaata ttgtaacctg ccccccacca gaggttctgg atcattttct
     8041 ttcgcgtttg cttggctaga tcaagaaaat gcaaaatatc ttttggatcg ctgtttgtca
     8101 atacgacatc cgcagaatcg attgcgatat ctgtccctgc accgattgcc atcccgattg
     8161 aagcacgggc taaacttggc gcatcattga tcccatcacc caccatgatt acttttttcc
     8221 cttggtcaag gtatttctgg acaacagctt ctttatcatc tggtaataaa ccgccataat
     8281 attcttcgat ccctaaataa tctgctactg cttgggcagc gtttttattg tcaccagtaa
     8341 gcatgacagg agtgattttt ctttctttca atgttcggat aaattctttt gcttctgttt
     8401 ttactacgtc acctaaagca acgattgcag ctagatgatt ttctaaaatc aggaagctga
     8461 tggtatttcc ttgttcttgg taaggtttca ataatgcttg ttctacgttt aatccgagac
     8521 gctcaacttc tttttcatta actattttca cttgttgttt tttgactgtt gcctctagcc
     8581 cgactcctga aagatttttt agattttcag cttgataagg cttgatttca tgttctttca
     8641 aataattcat aatgccaaca gccaatggat gattggcatt cgcttccaag gctcctagat
     8701 attgaaggat ttcattttta ttgaacgcat catcaagtac ttcgacaccc gttacagtaa
     8761 attttccttc tgttagtgtt cctgttttgt ccagcatgat cacatctaga tcattggctt
     8821 gctccagtgc attgcggttt ttcaaaagta atccgttttt agctgctatt gatgtggaac
     8881 gagcaacaac taatgggata gctaaaccta gagcatgagg acaggcgatg ataaagactg
     8941 ttaccatacg ttctaatgca ttaggaagat ctgtcaagaa caaccaggcg atgaatgcca
     9001 ggatacccac aattaatgcg acataaaaca gccattttgc cactttgtca gataatgact
     9061 ctagcttgga tttttctcct tgtgcttttc gcaccatttc cataaccttc gctagataac
     9121 cattttcacc agttccagtt acttcgattt cgatcgttcc atccccattg atagaaccgc
     9181 caatcacttt gtcattggct tgttttttga ttcctcttga ttcgccagtc acagctgatt
     9241 cgtcaacaat tgtttcacct ttcaagatta caccatctgt tggcatttta tcaccagacc
     9301 ggacgatcaa acgatcacct tcgtttactt ctttcaatga aactgtctct tcttttccat
     9361 ctttcgtcaa gcgtttcact gattctggca gtaattctgc tagtttttgt aaagcattgc
     9421 tggcattcga cacagcattc atttcaatcc agtgacctag tagcataata acgatcaaag
     9481 tagccaattc ccagaagaaa tccatgacgt gtgtatgtgg attgagcaga tttgcgataa
     9541 acgaatagac actataaata tatgcaactg taatccccat cgcgatcagt gtcatcatag
     9601 caggactttt ttgtttcaat tccatcttag ctccacttaa aaatggctga ccgccataga
     9661 taaatagtat agttgctaag atcaatacga gccaatcaga accaggaaac gttacttgaa
     9721 atggaaattc cattcccatc attggtgaaa aaagaatgat tgggatagct aggataagcg
     9781 atagccaaaa tttttgtttg aaattcccca tgtgcatgga atgatccatg ccgccgtgtt
     9841 cgtgatccat gtgcatcgaa tggtccattt gtttgctgtt tgctatatct ccatggttat
     9901 gtcccatgtg catggaatga tccatctgac tatggtcatg tttcatctcc atctgttttt
     9961 catgagattc tgtactttct ttgtttacta cttctttttt atgctcatgt tttttctcca
    10021 tatcgttccc attcattttt actccatccc ttctcttact ttttcatgtg tcgatttaag
    10081 cacagggagt tcaacaatac actgacggaa ctaaacgcca tcgctcctcc tgcaatgatt
    10141 ggattcaaaa agcctagtgc ggcaaaagga ataccaatcg tattatagac aaaagcccag
    10201 aaaagatttt gtttgatttt tctcaatgtc aacctagaca actcgattgt ttgtgcaatg
    10261 gatgccaaac tgctattcat cagcgtcaca tcggctgttt ccatcgcaat gtccgttcca
    10321 cttcccattg ccataccgac atcagctaat gctaaggccg gtgcatcatt gattccatcg
    10381 cctgccatac cgactttttt gccatctttc tgaagttttt ctacgtaggc tgctttatcc
    10441 tctggaagta cttcagcaaa gatatgcttt ggatcaatac caacttgttt accaatcgtt
    10501 tcggctgcca gtttgttgtc tcctgtcagc ataaaaacat ctaatccttt attctggagc
    10561 tgcttgattg cttgtttgac ttctagtttg atttgatcag ccacggcaat caacccgatc
    10621 acttgctttt catcagctaa aaacatgact gtttttccct gctgttctaa ttctaacgcg
    10681 tgttcttggt attcatcaaa tgataggttc aattcaatta atcgtttacg tgtacccgca
    10741 aaatagcgta ctccgttaat cgtaccgctg attccagcac ctgggtgggc tgtaaaatca
    10801 atcatatcgt atgttgctgc ttcttgcagt tttccgtatt ccacgattgc tttacctagt
    10861 ggatgttcag aagcttgttc taaagtataa aaaatagata ggacttcttt agaaccgact
    10921 agatctgtaa ctttgggctt tccttctgta atcgttccag ttttatccaa aacaatgctg
    10981 tctaagtgag cagctgcttc taatgcttct ccgcctttaa ttaaaattcc atttcgtgca
    11041 ccaacacccg ttcctaccat aatagctgtt ggagtcgcta aacctaatgc acatggacac
    11101 gcaatgacta atacagagac actatggatc aatgcgagtt gccaatcacc agtgatcaaa
    11161 cctgtagcta tgagcgtgat aaacgcgatg actaatacga tcggaacaaa aatccctgag
    11221 atccgatctg cgattttttg gattggggct ttgctccctt gagcatcttc caccatttga
    11281 atgatttgcg ctaatactgt ctgcttgccg acttgtgtaa cttccatgtg aagtagacca
    11341 ttcgtattga ttgtcccgcc aaagagcgtg tcatccggat tcttttctac aggaagattt
    11401 tcaccagtaa gcatactttc atctatcgca ctgcttcctg aaataattcg accatctgct
    11461 ggaatctgct caccaggacg gatgactagt tgatcaccaa cgactacttc ttcaatcgct
    11521 aacgtttctt ctttcccgtt tcttatcact tgggcggttt ttgtttgaag agacatcaat
    11581 tgtttgattg cattacctgt tttcgttttt gctgtatgtt ccaaatattt gccgagcaaa
    11641 atcagcgtga tgatcatact gctgctttca aaataaagat cttgggggtg tccacggaaa
    11701 aatccgttgt aaatgctcaa tgcaaaggca gccgatgttc caattgctac caatacatcc
    11761 atatttggtg ctttcgtctt gattgcatgg tacgcacctt tataaaaacg agcaccgaca
    11821 taaaattgga caggagcaga cagcactaac tggacgattg gaaaatgaaa aaagtgaaca
    11881 atcgccgcat ggcttcctaa catcatagca atcattgaca acatcaacgg caaagtcaaa
    11941 accgtactca atataagatc gaatagcatt tttttcaaat acgcttgctt ttcttctgca
    12001 attttttgtt tgtgggcttc atcgtacaaa attgcaccat agccgatgtt ttctacgctt
    12061 tggattaatt tttcagtggt cgtcccttca tatttgacac ttgctttttc agtggccaag
    12121 ttgactgtgg cattgaccac accaggttgt ccgttcagct ctttttcgat cctagcagaa
    12181 caatttgcac aagtcatacc tgtaatgacg aacgtatcca ttttggtttc tgctgttgcc
    12241 atcaaatcac ctctgcttta taacccaatc cattgattgc ttgacaaatc ttttcggatt
    12301 gaacttttgt ttcatcaaac ttgaccgtac cgtttgcttt tttcaaattt actttcactt
    12361 tttgcacgcc gtcaagtgcg cttactgttt cttctactct tgctgcacaa tgattgcagc
    12421 tcataccttc gattgaaaat tgttgtttca ttttttcttc ctcctattga tgttgtttac
    12481 attcacattg acctgggata cagttacatt cgatcgtatc tactggttgc ttttgttcca
    12541 gttgtttcaa aatctcattg atatcttctt gagtcaaagt ggcttcttct actagatccg
    12601 caatcgtctc tccaatcctt tttgcacaga tatgggaaaa aagattttcc gtcgcactgc
    12661 gaactgtttc catctccgag acagctggat aataaatgaa tttttttcct tcttgttctg
    12721 tccagatcac ttcttttttt accaaacgac ccagcaaagt tttgatcgtt gctggtttcc
    12781 aatccatcga atcagctaag atctgcgtga tttcttgggc tgtcgtctgt ccaagtgtcc
    12841 aaataacacg catgatctcc cactctgaat cactgatctt tactggcgtt ttgacttttt
    12901 ccatttattt cacacctttc gtttacaaat gtaatcatta tcttatgaat acagattaca
    12961 cttgtaaaca aaaactgtca attatttttg ctctctattt tcaatttagt taaaaattct
    13021 tgtttctctc gtcttgtttt aaggatgatc agtttatttc ttgcattagc tacttggatc
    13081 agctgctgta ttttcggttc attgttttct tcaaaatgcc agataaaaga taaaaattct
    13141 aagtattccc tgtttagttt ctccgaaaat gattcaggca tatctggccg agattttttg
    13201 tttatctttc tttttagaga acgccaaact actcggtaga tggcgagata acgcggcact
    13261 ttaagccaaa tgatcttatc tgcttcttta atcctttcct ctaatgtaga agtataattt
    13321 ccttcaacga tccatgacgg attagatacc ataaacaatt gctgttcttg caaaaaccac
    13381 ttttttgcct gaatactgta gtctgttgta tgccataaaa ggtctagcgg cagtagcgga
    13441 aagtcggtga tttctttgat cttccttgcg agggtcgatt ttcccgaccc actttgtcca
    13501 atcacgatga tcttcattct tctccctcac ttctctttgg aatttggttc gatcatacca
    13561 cttataacgc cgcaaaaaaa caagggactg tgacaaaagt cgtgtcaaag tcccttgttt
    13621 cgaataa
//
LOCUS       DAYYAO010000071        13490 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000071 DAYYAO010000000
VERSION     DAYYAO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.074"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..803
                     /locus_tag="ACHARI_002070"
     CDS             156..803
                     /locus_tag="ACHARI_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8443092.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    932..1148
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1196..3841
                     /locus_tag="ACHARI_002071"
     CDS             1196..3841
                     /locus_tag="ACHARI_002071"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8443093.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4151..5464
                     /locus_tag="ACHARI_002072"
     CDS             4151..5464
                     /locus_tag="ACHARI_002072"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8443094.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5454..6107
                     /locus_tag="ACHARI_002073"
     CDS             5454..6107
                     /locus_tag="ACHARI_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8443095.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6162..6854
                     /gene="radC"
                     /locus_tag="ACHARI_002074"
     CDS             6162..6854
                     /gene="radC"
                     /locus_tag="ACHARI_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8443096.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7004..7204
                     /locus_tag="ACHARI_002075"
     CDS             7004..7204
                     /locus_tag="ACHARI_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8443097.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7458..9137)
                     /locus_tag="ACHARI_002076"
     CDS             complement(7458..9137)
                     /locus_tag="ACHARI_002076"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8443098.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9139..9351)
                     /locus_tag="ACHARI_002077"
     CDS             complement(9139..9351)
                     /locus_tag="ACHARI_002077"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8443099.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9744..11474
                     /gene="efrA"
                     /locus_tag="ACHARI_002078"
     CDS             9744..11474
                     /gene="efrA"
                     /locus_tag="ACHARI_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8443100.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11471..13231
                     /gene="efrB"
                     /locus_tag="ACHARI_002079"
     CDS             11471..13231
                     /gene="efrB"
                     /locus_tag="ACHARI_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8443101.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         4264 a         2346 c         2903 g         3977 t
ORIGIN      
        1 atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca ccgttttatt
       61 tgtctcacac ccttttcctt cctacttgta accttgctcc cgtgttagaa acatgttaca
      121 ctaaatatgt gaaattatta acaaggagcg tcgttatgcg agaaaaaaca gatacactgc
      181 caaaagcaac tgcgaaaaga ttacctttat atttacgcta cttgaagatg ctggatgact
      241 caggtataag tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt ccttctgcaa
      301 cgatccgcag agacttctcg caattaggcg agctaggcag aagcggatat ggctatgatg
      361 tcccttttct tatcgatgta ttcaataata tccttaatac taaagaagaa aaacgaatcg
      421 cattagtagg atatggaaat cttggcaaag cattgaaaca caacaatttt agaagaaatg
      481 aaaatctaaa tatcgtgtgc gtctttgata atgatccagc actgatcaat cgagtaattg
      541 atggagaaat gatctatccg attgatcgtt tcgcggagat agccaaagca aaaaatgtga
      601 cagtagcaat ctctacggta ccaagtaaat attcacaatc tgctattgat gaaattgtca
      661 aaggaaatgt aaccgctata cttaattttg cgcctgacag agtaactgtc ccagcttatg
      721 tcaatgttca atacattgat ttgacaaccg agttgcagac gcttatttac tttgatgaga
      781 attactcaga agttttttct taggaaaatt tgagtaggct tattgatctt gcgagaactc
      841 gtcgtaattc tttcaaaaat tttttagtac taaatgaagt ggttttttca cgaccacttg
      901 ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga ggagtaactg
      961 gcacattcat tcttagagag agaactatct gctgaaaggt tctcaatgac aaccagtgaa
     1021 ggtagcttgg gagtttctgt cttgaatgga gtagagatag acgagtcgtg atcgttaact
     1081 cacttgagag gcaataatga ttgattattg caatttaggt ggtaccgcgt aaatcatacg
     1141 tcctagaata cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa acagcatgac
     1201 ggaagaaaat aacctatcaa caaaatataa tccgcaagaa gttgaagctg gacgttatca
     1261 gcattggtta gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa aaccttattc
     1321 aatcgtaatc ccgcccccaa atgttactgg aaaacttcat ttagggcatg cttgggatac
     1381 gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata ctttatggct
     1441 gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga agcttagaga
     1501 gcaagggatt tctcgttatg atctaggtcg tgaaaagttc atcgatcaag tgtgggaatg
     1561 gaaagaagag tatgcgagcc acatccgcga acagtgggct aaaatgggct tatctttaga
     1621 ttacagtagg gaacgtttta cattagatga tggattatct gaagcagtac gtaaagtttt
     1681 tgttacctta tacgaaaaag gtttgattta tcgtggtgaa tatatcatca actgggatcc
     1741 tcaagcacga acagctctat ctgatatcga agttattcat aaagatatcg aaggcgcctt
     1801 ttatcatatg agctatgaac tagcagatgg atcaggcgta gtagaaattg cgacaacccg
     1861 tccagaaacg atgttgggag atacggcgat tgctgttcat ccagaagacg agcgttatca
     1921 agcgctcatc ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc caatcattgc
     1981 agatgaatat gtagatatgg aattcgggac aggtgtagta aaaatcactc cagcacatga
     2041 tccaaacgac tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg ttatggatga
     2101 tgatggtacg atgaatgaac tagcaggaaa atatgctgga atggaacgtt ttgctgctcg
     2161 taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg atacgatgat
     2221 ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt gaacctcggc tgtctactca
     2281 atggtttgta aaaatggcgc cgttagcgaa aaaagccatt gataaccaag aaacagatca
     2341 agcagtggaa ttcttccctc caagattcaa tcaaacgttt ttacgttgga tggaaaatgt
     2401 ccatgattgg gtaatctcac gacaattatg gtggggacat caaattcctg catggtatca
     2461 caaagaaaca ggtgaaatgt atgtagggat ggaagcacca gcggatagtg aaaactgggt
     2521 acaagatcct gatgttttag acacttggtt tagttcagct ttgtggcctt tctcaacaat
     2581 gggctggcca gatacggaaa gcgaagatta taagcgatac ttccctacta gcactttagt
     2641 aaccggttat gacatcattt tcttctgggt aagcagaatg attttccaaa gcttggaatt
     2701 tactggcgaa cgtccatttg agaatgtctt gattcatgga ttgattcgtg acgaacaagg
     2761 gcggaagatg agtaaatcat taggtaatgg aatcgatccg atggaagtaa tcgaaaagta
     2821 tggagcagat gctctccgct ggttcttatc gaatggttct gcaccaggac aagatgtccg
     2881 cttcagctat gaaaaaatgg atgcttcttg gaactttatc aacaaaatct ggaatgcttc
     2941 acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa gagatcgatt tatctggaga
     3001 aaaatcagtg gctgaccggt ggatccttac tcgtttgaat gaaacgatcg aacgagtaac
     3061 tgagctattt gatcgttttg aatttggtga agcaggtcga caactgtata actttatctg
     3121 ggatgatttt tgtgactggt acatcgaaat gagtaaagaa gtactttatg gagatgatgc
     3181 tgtttcaaaa caaaccaccc gtagcatctt agtccataca cttgatcaga tcctacgttt
     3241 attgcatcca atcatgccat tcgttacaga agaaatatgg gaacatattc ctcatcaggg
     3301 aaattctcta gtagtggcag aatatcctgt tgtacgtcca gagtttgatg atgaaacagc
     3361 ttctaaaggg atggaagtat tgaaagaatt gattcgagcc gttcgaaata ttcgttcaga
     3421 agtgaataca ccattgtcta aaccaattac gttgctaatt aaaacaaacg atgctaaaat
     3481 cgatcaattt ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc ccgaagagtt
     3541 gacgatatca agtgagatca ctgcgccaga tctagcgatg tcagctgttc tgacaggtgc
     3601 agaaatcttc ttgccgttag caggattgat caatattgaa gaagaaatca aacgattaga
     3661 aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac tttctaatga
     3721 acgttttgtt tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa aagaaaaaga
     3781 ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta ctattcaata
     3841 agaatagaaa aagagagtgt gaaatcagct agctattact aaaagctagc tgattgaaca
     3901 tcgttacagg aaagagtttg agcctcactc ttagcttcga ttcataagga gatgtgacat
     3961 aaattttgtc gcatctcttt tttagtctac tcttattatg taggagaagg agggaggtgg
     4021 atcatgtatc aagagtcaat aaagtagttg ataaaatcgt tcctctctct gtttcttttc
     4081 tctttgtcat acttttattt ttcttatgtg ttctatttgt tataataaaa gagaaatggg
     4141 ggaattgttg atgacgattg aagaagcaat agaatggatc catagtcgat taccatttgg
     4201 ttcacgtcct ggccttgacc gagtcgaagc tttattagaa gtggtagggc atccagagag
     4261 aaaagtaaaa acgatccata tcgctggaac aaacggaaaa ggttctactg tagcttattt
     4321 gagaagccta ttagaagagg ctgggctgac agtaggtaca tttacttcac cttatatcga
     4381 aacgttcaat gaacggatcg caatcaatgg caaaagcatt ccagacaatg atttgatcca
     4441 gcttgtaaaa aaatatcagc cactgatcaa acaacttgat caatttgaag cagtagctgg
     4501 tattactgaa ttcgagacat taacagcaat ggcatttgat tattttttga tgaagaaagt
     4561 ggacgtagcg attatcgaag ttggcttagg cggcttattg gattctacca atgtagctgt
     4621 accaatgctg acgggaataa caacgattgg acttgatcat accgaaatac ttggagatac
     4681 aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc cagtagtaac
     4741 aggaaatatt gccccagaag cttttactgt catccaacaa acagctaaag caaaaaatgc
     4801 accgctttat gtatacgggg aaaattataa aataaactat ctgcatcctg atgagatgtg
     4861 gggagaagta tttgaatttt ttaatgaaat aggaaagatc cctcaattaa ccactccgct
     4921 tttagggcaa catcaagctg aaaatgcagg agtagccatc gaattgtatt ttgtttactg
     4981 tcaattggaa aagctgcctt tccgagaaaa agatgttttg aagggattga agaaatctcg
     5041 atggccagga agaatggagc gtttgagtaa cgagccatta gttgttttag atggtgccca
     5101 taatacgcat gcaatgaacc ggcttgttga gaatttgaaa aaagaattca aaggatatca
     5161 aatcaacatt ttgttttccg ccttacgaac taaaaatgtt agagaaatgc tagaacgatt
     5221 gcagcaagta ccaaatgtcc atatttattt gacgaccttt gaacatccaa aagccatcga
     5281 attaagtgaa gtatcagatt tagcaagtga gagagtatca atcgtttcat tgtggcaatt
     5341 cggattagca gatatattgg aaaagatgac aaatgaagac gtattagttg tgaccggttc
     5401 actttatttc atttcagaag ttcgccattt attaatacaa ttaggaggag atcatgaagt
     5461 ataatgcagt gatttttgac atggatggtt tgctgttcga cacagagtta gtgtattacg
     5521 aagccacaca agtagtagca gacaagatgg gttttcctta tgataaagac ctgtatttaa
     5581 aattcttagg tgtttcagat gaagaagtat gggcgaatta tcatatgatt ttctcggaat
     5641 ttggcaaaac aagtgtacaa agattcatag atgattcata ccaggaaacg cttgaacgtt
     5701 tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga actcctctct tttttagaag
     5761 aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt gaacttttat
     5821 tgaagaagaa caatctgttt gaacgatttg agacgattgt ttctgcagaa aatgttcaac
     5881 gagcaaaacc agacccagaa attttcttgt tagctaatca gaagctaggt acagcgaaga
     5941 aagaaacatt gattttagaa gattcacaaa atggtatttt agcagcttct gcggctgaga
     6001 tcccggtagt catggtacca gatctgcttc caccatccca agaattgatc aaaaaaacag
     6061 ctggggttgt ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa tccatctctt
     6121 ttcgtatctt attttagata agatagaaag gagatgtttt tttgggaaaa cgattgatca
     6181 aagaagtacc tactagctct ttaccaaggg aacgtatgga aatttatggc gcggaagctt
     6241 tatcggatca ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct tatagtgtga
     6301 tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact ggcctcttta cgacaggcta
     6361 ctttgcatga gttagaagag attcgaggga taggacgcgt gaaagcaatc gaaatcaaag
     6421 ccttgatcga attaggtcga cgaattcaga cggatcatga aagagcggct ccaccggttc
     6481 gtaccagcta cgaattggcc cagcaattga ttctggaaat gaaagatcac aaacaagaac
     6541 atttggtttg tatttatttg gacacgaaga atcaagtgat cttaaaaaaa acagtgttca
     6601 tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat gctgtacgtt
     6661 attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt attccatcgc
     6721 agcaggatat gaattttacc aaaaggatac aaaagtgtgg agaaatgatg gggatcactg
     6781 tactggacca cttgatcatc ggacgcaaac gatattttag tttgagagaa gaaggaatga
     6841 tggaagagaa gtaagttgct ttcttgcaaa aaaaatgaag caagtgtaca attatattgt
     6901 aattttgttt gggaatagaa gttgaaacat ctgttgcacc tctatcgtac acgagcaatg
     6961 ttacaattta aattagtgct tttggcacga ggaggatatt attatggaaa acggaacagt
     7021 aaaatggttc aacgctgaaa aaggatttgg atttatctca cgtgaagatg gaagcgacgt
     7081 attcgttcac ttctcagcta tccaaggtga cggattcaaa acattagaag aaggtcaagc
     7141 agtgactttc gacgttgaag aatcagaccg cggcccacaa gctgcaaacg ttgtaaaagc
     7201 ttaattttat ctgtaaatca aattgatggt agacatcaat ttttacgaga gaacaaagcg
     7261 agtaacaaaa atacctgcca caagagacaa tttctcttgt ggcaggtatt tttttgtatg
     7321 aaaaagtaaa taaatttaag aggctatgaa agagccacct tgtatcaagg aataagaaca
     7381 aacaaaaatg gcttctcatg tttttcattt gttttctctt atattcagga caaggtgctg
     7441 atttcataac ctcttattta ttcttcatct ggtgtcatta ccattggcaa aatcattgga
     7501 tgtctttctg tacgttcgaa taagaaaggc tgaagcgcgc cagtcatcgc ttctttcaat
     7561 ttatattctg aacagtcagg tccattcaaa cattctctta aagcgtggaa taagattcgc
     7621 tgagcttcat tgatcatttc tcctgattct cgcatgtaga caaatccacg agagagaata
     7681 tctggaccag ccaagatttc ttttttcttg atatccactg tcgcgacagc taatacaaga
     7741 ccttcttctg aaagcaggtg acggtcacgt aaaacgacat taccaatatc accgatcccg
     7801 ctaccatcga tatacacatc tccagccaca aaatgcccag caggacgcgc agaatcgcta
     7861 gttaacgcta aaacatcgcc attttccatg atgaagcagt tttctgcagg aacacctaca
     7921 tcttgtgcta gagaagcatg tactttcaac atgcggaatt ctccgtggac aggaataaaa
     7981 tattttggct tcatcaaacg aagcattaat ttttgttctt cttgtccacc gtgaccggaa
     8041 gtatggatgt tattgatttt tccgtgaatc acttctgcac cagcttcaga cagcaaattg
     8101 atcaaatgat tgacacttgt tgtattacca ggaataggtg aacttgaaaa gatcactgta
     8161 tcgtctggct ggatttggat ttgacggtga gtaccattcg cgatgcggct caacgcggcc
     8221 atcgcttctc cttgagaacc tgtacatagg atcatcgttt cattggcagg caagcgattg
     8281 atttcgtgtg catctacaaa tgtgccgtct ggaactttga tatatcctaa acgttgaccg
     8341 ttgacgatgg cattttccat gcttcgtccg aaaacagcga ttttacgacc agttgccaca
     8401 gcggcatctg ctgcttgctg caaacggaag atgttagaag caaagctggc aaaaatgata
     8461 cgtccttcga tttttgaaaa gatcttcaaa atagaatgcc caatcgtttt ttctgattta
     8521 gtaaatgttg gaatctcagc atttgtacta tcagataaaa ggcacaagac tccttcttcc
     8581 ccgatacgag ccattttatg caagtttgct ggttcgccaa caggagtgaa atcgaatttg
     8641 aagtctccag tcgctacgat atttccagaa ggtgttttca ccacgatccc taatgcatct
     8701 gggatactat gggtcgttct gaaaaagctg acagaagtct ttctaaaacg gatgatcgta
     8761 tcttcattga tctcatggag ctctgcatct cgaagcaagc catgctcatc taatttgttt
     8821 gtgatcaacg ctaatgcgag aggaccagca tagataggaa tatttgcttg tttcagcaag
     8881 tagggtattc cgccgatgtg gtcttcgtgc ccgtgagtaa tcaccaaggc ttttactttg
     8941 tgtaagtttt gtatgatgta gctgtagtcg ggaatgacat agtcaattcc aagtaaatcg
     9001 tcttccggga atttgatccc ggcatcaatc aaaatgattt catcttggaa ttgtacccca
     9061 tacgtatttt tcccgatttc acctaatcca ccaatagcaa agacgcctgt ctcgttattt
     9121 ttgatggaaa gtttcatatt aaaactccac taatgagaaa tcagcatttt ctttttcgta
     9181 ctctagatga cttccttcta gaagttgaac gaattcaata ttgtatgggg tattttcttc
     9241 cacacgttgg cgagcgtcta catcgctttt tgcctcaatg taaagagatt ttgtttcttc
     9301 ccgtttaggg ttgcgtactt ttgtttcttg ataataaact ttatagatca ttgttttgtc
     9361 tcctttatct gcgtaaagca gtgatttagc tagttgattt ttttagacaa cacaaaacga
     9421 ggggacaagc cacctggttt ttcatatgct ttgactagaa tactggattc gagtcaaaga
     9481 gacaagcctc gtttttgctt atctgttcgg atattttttc aaccgtacct tctgtataaa
     9541 aaatatacaa caattaattg ttattctagc ataaaacagc aaataagtat agaaatcaac
     9601 tagattgaaa gaaaaaatca atgtttatca tcccgctata atcataatga cttttttatg
     9661 agagaagtgt tctattaata ggctcgttgt gctatcattt ttagtaatga aaatgtaaca
     9721 aaagattaaa aggagatgag aaagtgaaac taatgtggca ttataccatg cgttataaga
     9781 aattcttgtt tttcaatttc atatgcgtat ttggttttat tttgattgaa ttaggacttc
     9841 ctacgatttt agcacgtatg atcgatgtag gaatccgcaa tgatgatttt gattatgtga
     9901 agcaacaagg gatatttatg atcgttatta ctgtaatcgg tattattttg aatatcttgt
     9961 taggttattt tggttcgagg attacgacta atatcgtagc agatattcga gatgatttgt
    10021 tcgaaaagat ccaaagttat tcgcatcagg aatacgaaaa tctaggcgta tcttcattga
    10081 tcactcgtac aacaaacgat gcttatcaga tcatgttgtt cctgcaaaat attttacgta
    10141 tcggttttat ggcaccaatg atgttcattg tcagtttgta catggtcatg cgtacaagtg
    10201 tgacactcag tttgtatgta gtaggtgcat tacctttact attattggca gtcgtagcga
    10261 ttgctaaggt gtctgaaccg ttatctaaaa agcaacaaaa aaacttagat aaaatcaaca
    10321 gtatcttgcg ggaaaatctt tcaggattgc gtgtgattcg tgcattcgtc aatgaaaagt
    10381 ttgaagaaaa acgttttgct gtagtcaatg agaattatgc ttctagttca aaaagcttat
    10441 tccgtttgat ggctgttgca caaccgggtt tttatttctt atttaatatc gtaatggtat
    10501 tgatcatctg gagcggggca gtgcaaatcg atgcaggcga gcttcgtgta ggagatttga
    10561 ttgcgtttat cgagtatata ttccatgcct tgttctcatt catgttattt gctagtgtgt
    10621 tcatgatgta tccaagagca gctgtttcag ctggacgtat ccaagaagcc ctagatgcca
    10681 aaccttcgat tgtagaagat ccaaatggta tatctgatac aaaaactaaa gggtatatcg
    10741 aatttcaaaa tgtaacattt gcttatccag gccattcgca agagccagtg atacgaaatg
    10801 tcagttttac agcctctcca ggagagaccg ttgcttttat tggaagtacc ggtagcggaa
    10861 aatctacgtt gatccaattg atccctcgct tttatgatgt gagtgaagga aagatcttga
    10921 tcgatggagt agacgtgaga gattatcggc taagcaagtt gcgagataag attgggttta
    10981 ttccgcaaaa agcgctgcta tttactggaa cgattgcaga taatttgcga tacgggaaag
    11041 aagatgctac gcaagaagaa atggaacgtg cagctgagat tgctcaagct tcagaattca
    11101 tttctcgaaa accagatgga tttaatgaac atttatcaga aggtggttcc aatttttcag
    11161 gtggacaaaa acaacgattg gcaatagcca gagccatcat acgccgtccg gaaatctata
    11221 tttttgatga cagcttttct gcattagatt atcaaacgga tgcgaagtta cgtgctcgtc
    11281 tgaaaaaaga aacgacagaa tctactgtat tgatcgtagc acaacgtgtg ggaacgatca
    11341 tgcatgcaga taagatcatc gtattaaatg aaggagaagt cgtcggtatg gggacgcatc
    11401 gggagttgtt ggaaaaatgc ccgatttatt atgatattgc cgcttctcaa ttatcaaagg
    11461 aggaattagc atgaaaaaaa ccttttcttc cttaaaacgt ttagctcgat atatcagacc
    11521 gtatcgtgag acttttcttt tagtgatcct atttacagtt ctgacagtgg ctttcaacgt
    11581 ggctttgcca tatgttacag gacttcctac tacagaaatc agccgtaata ttgccaatgg
    11641 cgaatcaatc aatttttctt atatattcaa atgtctcatt tggatcacag ttgttggaat
    11701 agggtattgt atttcgcaat tactttctgg tgttttgatg acaaatgtcg ttcaaagtgc
    11761 catgcatgat ttgagaagag acatagaaga aaaaatcaat cgtcttccgg tttcatattt
    11821 cgataaaaac cagcaaggaa atattttgtc tcgtgtgacg aatgatgtag atgcagtcag
    11881 cggtgcttta caacaagctt ttattggtat cgtaaatgcg atattgggaa ttacgttggc
    11941 tgcagcaatg atgttttata tccagccgat gatggcgctg atctctatga tcatgattcc
    12001 aacatcgatc tggatctcaa aacgagtgat caatgcttcg cagaaatatt ttcaaagcat
    12061 gcagaattct cttggtgagc taaacggcta cgtccaagaa aatatgacag gttttagtgt
    12121 attgaaagta tatggacgcg aaaaagaaac attggaagga ttcaaaaaag ttaatcacag
    12181 cttgaaatac tttgggtttc gtgcagcatt catttctggc ttgatgatgc ctctagttca
    12241 attaactgcc tatgctactt atattggtat ggctgttcta ggaagctttt atgttattac
    12301 tggggcgatc gttgtcggtc aactacaagc attcatccaa tatatttggc aaatcagtca
    12361 gccaatgggg aacgtgacac aattgtcttc agttttgcaa agtgcatcag cggcaacgaa
    12421 acgtgtattc gagattttag atgagccaga agaaaaagaa aacaaagtag atgcttcact
    12481 gccagaaacg attttaggag atgtgacatt cgatcatgtc gattttgcat atgatccgaa
    12541 gaaaccatta atccaagatt tgaattttga agtgaaagct ggtcaaaccg tagctgtggt
    12601 cggtccgacc ggtgccggca agacgacatt gatcaatcta ttgatgcgtt tttatgacgt
    12661 agacaaagga gcaataagga ttgatggaat cgacaccaaa tcaatgagtc gcagtgatgt
    12721 gcgttcgcta tttggtatgg tcttgcaaga tgcatggctt tatcaaggaa cgattgcaga
    12781 taatatccgc ttcggaaagc ttgatgcgac agattatgaa gtagttgatg ctgcgaaaac
    12841 agcaaacgta gatcatttta tccgaacgat gccagatgga tatgatatgg ccatcaattc
    12901 tgagggagac aatgtgtctt taggacaaaa acagttgctg acgattgccc gagcagtggt
    12961 ctccgatcct aaaatcttga ttttagatga agcaacaagt tcagtagaca cccgtttaga
    13021 agcgttgatt caaaaagcaa tggatcgcgt gatggaggga cggacaagtt tcgtcatcgc
    13081 tcaccgttta tcgacaatcc gtgatgcaga tctgattcta gtgatggatc aaggtcagat
    13141 cattgaaaaa ggaacgcatg agagtttatt ggcacaaggt ggattttatg aaaaactgta
    13201 taacagccaa tttgctgagg aagcagaata agtcatataa gaataaaaca gtttaaaact
    13261 tattttttgt tctctattct tgataatggt aaagtaaagt tatcaagaaa agtgaggaca
    13321 cggagatgga acagttttta aatgaaaaaa ttactgaagc caaaagagaa gttgatcgtt
    13381 taacgcacgt ctgtcagacg aagccttttg attcagctga tttttttgaa aatgagtttc
    13441 aaatcgaacg cttggcagca caaatagaag tgtacgaatt aattttaaaa
//
LOCUS       DAYYAO010000072        13456 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000072 DAYYAO010000000
VERSION     DAYYAO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.075"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACHARI_002080"
     CDS             4..2376
                     /locus_tag="ACHARI_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8443102.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACHARI_002081"
     CDS             2437..3897
                     /locus_tag="ACHARI_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8443103.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACHARI_002082"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACHARI_002082"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8443104.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACHARI_002083"
     CDS             6128..6277
                     /locus_tag="ACHARI_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443105.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACHARI_002084"
     CDS             6352..6945
                     /locus_tag="ACHARI_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8443106.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACHARI_002085"
     CDS             6958..7857
                     /locus_tag="ACHARI_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8443107.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACHARI_002086"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACHARI_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8443108.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACHARI_002087"
     CDS             8723..8983
                     /locus_tag="ACHARI_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443109.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACHARI_002088"
     CDS             9428..9634
                     /locus_tag="ACHARI_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8443110.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACHARI_002089"
     CDS             9655..9885
                     /locus_tag="ACHARI_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443111.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACHARI_002090"
     CDS             9901..10338
                     /locus_tag="ACHARI_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443112.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACHARI_002091"
     CDS             10331..11038
                     /locus_tag="ACHARI_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443113.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACHARI_002092"
     CDS             11202..11402
                     /locus_tag="ACHARI_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443114.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACHARI_002093"
     CDS             11418..11597
                     /locus_tag="ACHARI_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443115.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACHARI_002094"
     CDS             11922..12185
                     /locus_tag="ACHARI_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8443116.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACHARI_002095"
     CDS             12179..12529
                     /locus_tag="ACHARI_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8443117.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACHARI_002096"
     CDS             complement(12553..13167)
                     /locus_tag="ACHARI_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8443118.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4979 a         2211 c         2752 g         3514 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttctta
//
LOCUS       DAYYAO010000073        13263 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000073 DAYYAO010000000
VERSION     DAYYAO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.076"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..464)
                     /locus_tag="ACHARI_002097"
     CDS             complement(<1..464)
                     /locus_tag="ACHARI_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8443119.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGC"
     gene            complement(475..1143)
                     /locus_tag="ACHARI_002098"
     CDS             complement(475..1143)
                     /locus_tag="ACHARI_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM8443120.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1274..1858)
                     /locus_tag="ACHARI_002099"
     CDS             complement(1274..1858)
                     /locus_tag="ACHARI_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8443121.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2193..2423)
                     /locus_tag="ACHARI_002100"
     CDS             complement(2193..2423)
                     /locus_tag="ACHARI_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8443122.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2438..3388)
                     /locus_tag="ACHARI_002101"
     CDS             complement(2438..3388)
                     /locus_tag="ACHARI_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8443123.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3385..4227)
                     /locus_tag="ACHARI_002102"
     CDS             complement(3385..4227)
                     /locus_tag="ACHARI_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8443124.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4360..5085)
                     /locus_tag="ACHARI_002103"
     CDS             complement(4360..5085)
                     /locus_tag="ACHARI_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8443125.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5546..5860)
                     /locus_tag="ACHARI_002104"
     CDS             complement(5546..5860)
                     /locus_tag="ACHARI_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8443126.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(5876..7471)
                     /locus_tag="ACHARI_002105"
     CDS             complement(5876..7471)
                     /locus_tag="ACHARI_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFM8443127.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7494..8384)
                     /gene="iolE"
                     /locus_tag="ACHARI_002106"
     CDS             complement(7494..8384)
                     /gene="iolE"
                     /locus_tag="ACHARI_002106"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFM8443128.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8401..9426)
                     /gene="iolG"
                     /locus_tag="ACHARI_002107"
     CDS             complement(8401..9426)
                     /gene="iolG"
                     /locus_tag="ACHARI_002107"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFM8443129.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9450..10457)
                     /locus_tag="ACHARI_002108"
     CDS             complement(9450..10457)
                     /locus_tag="ACHARI_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8443130.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10570..11730)
                     /locus_tag="ACHARI_002109"
     CDS             complement(10570..11730)
                     /locus_tag="ACHARI_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFM8443131.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(11806..13260)
                     /locus_tag="ACHARI_002110"
     CDS             complement(11806..13260)
                     /locus_tag="ACHARI_002110"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFM8443132.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         3961 a         2876 c         2177 g         4249 t
ORIGIN      
        1 ctacatccaa tagaaatttt ctgccgttca ccacttgata gcgaaaaaat gtttttatct
       61 aatatatttg aaagatctaa taattcagct aattcattga ttcttttatc catatgctct
      121 tttgtaaatc caaagttttc catcgaaaat actaactcac tcgttgtatt tgttgtaaag
      181 aattgtcctc taggatcttg acttacgtag ccaatatttt ttgatatact ttcttgtgtt
      241 aaggattgta gcgagttatt ttcaaataaa atttccccaa ccaattttcc gtcataaagt
      301 cttggaataa gtccattcat taactttaat agtgtggatt tcccactccc agaagaacca
      361 gtaatcaatg taagactacc tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc
      421 ttaacatcct tatatgaaaa agaaacattg ttaaattcaa acaattcttt cacctcaaat
      481 caaaatactt aagaaaaaaa tagtaacgga aaacaggatg atatagtcta ataagctcat
      541 gtttgtttta tagtaagaac ttcttttcac aggagattct atcccctttg ttattgcatt
      601 agcggtcatt tcttcagcaa tcatagttgc acgtataaga acgggaacta acagaaattc
      661 caaatattgc agaggctgaa aaaaagacaa gcgaatgttt ctcattttta aagattcttt
      721 aatcattctt atttcagaac gataagtcgg aaagaatctg atcactactg taatacttaa
      781 cattattttt ttcggtaaat gtattttttc taaagaggat aataattcat tgctagtata
      841 gacactaacc aacaaccatg agatcattac gaccggcaac attcgaaata caatatataa
      901 ggatgtccca aaaaatatag taaaaaactc tatcttgata gcatcaataa ttttcattac
      961 taaaaacaga actgaataaa tcaagatgaa ataaatacat gtctttattt tacgaaacat
     1021 caatactaaa actgtagcaa taatgaaaca ccccacttga aatatatttc cctttcctaa
     1081 aaaaataatg accggaaaaa aaatatttaa agtaatcaag gttaacggat tgatttttga
     1141 cactctttca cctccaaaaa ataagatcgc actgttatct taagctgata aaaatgcacc
     1201 gttattcaga taacagtgcg atcttatcaa aaagcataac tgagaacaat tctcaatgca
     1261 agcttttttt taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc
     1321 ttctggttaa tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg
     1381 tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat
     1441 tgatcattcc gatagtcttt aacttatcta gacaacagac tgtctttttt gtcacaaatg
     1501 atgtaatttt ttcaacaata tcttgatatt tggttccagc tgattggaat aaaaccaaat
     1561 cgaatatacg tttattctca aaaatataat taataaatgg atctaattca ttgaagttct
     1621 ctctccaact tttctctata tctatatttt ctaagtttaa gaggtatttg gtaaactctt
     1681 cattatacat attaataagc gcattaatat gcggctgaat aatttcttca aataacgctt
     1741 ccttagaagt aaaatgttta taaaaagcac ccgtagttac gcctgcggtc aagcacagtt
     1801 gccttatctt agtttgctga aatcctactt tttcaaaatg gcttagtcca ctttctatta
     1861 atttttggtg agttgtaata taatctctgg tttctatggg tacttcctcc taataagatg
     1921 aattaactat attatcttac acaggacttg aagtttcaat cgttaacgtc tattactgaa
     1981 ctcattcctt agagctaaac taatgagaaa gagcaaaata actgatacaa aaagcaatcc
     2041 tacaagaatt aatagactca tctaaatcat tcaccttttc taaaataatc cgagaaagat
     2101 ttaaaaaaat tatatccagc tattaggata agtcctatca taatcactcc aatagccatt
     2161 ttcttatcct ccctaaaaat aaagcctttt acttaaaacc tttttttgta atcgtcaata
     2221 aattttttcc ctttatcagt taattgaaat cctatatcta aatagtcctc atatctaagt
     2281 ttagtcagca tctcacttac agatgctgga ctaacactta gaacttttgc taaatcatta
     2341 ttttttaatt tcttttttct ttttgataat tcctgaattg ctagtaaata aattagtata
     2401 gtagttgttt taatttgtac catacctacc ctccaaatta tttcgaaagg ccttcagcaa
     2461 ttttatccag gttccatttc atcatcgcat agtaactatc accatcttgt cctttttctg
     2521 caattgaatc agtaaaaatc gttgaataga taggaatatt ggtatctttt gatactgttt
     2581 tcattggtct atcgtccaca ctgctttcta cgaataagga gggaactttt gttgtgcgta
     2641 atttttcaac taagtgtttt atttgatctg gtgttccttc ttcttcagtg ttgatttccc
     2701 aaatgtaagc agaaggcaca ttatacgctt tcgagaaata tttaaagcat ccttcacttg
     2761 taacaatcat ttttttatca ttcggaattg aagcaaattt agatttagct tctttgtcta
     2821 gagaatccaa tttttcaata tacttatcta gattttcttt atagaatttt ttattatcag
     2881 gatctttttc cgctaactgt ttttcaatat ttttagcgta aataataccg ttttctaaat
     2941 ttaaccaagc atggggatct tccttccctt tctcactctg accctctaag taaataacat
     3001 ctatgccatc acttgctgca aaatagtctt tgttttcctc tttgttcgca ttttttacta
     3061 attttgtaaa ccaagcattt cctccagttt ccaagttaac accgttataa aaaatcaaat
     3121 ctgcttttga agttttttga acatcttcag gcaaaggttc atattcgtgg ggatctttcc
     3181 caataggtac gatactgtgt aaatcaattt tatcttttgc tatattttca gtaatatctg
     3241 ctaaaatcga attagtaact actaccttta atttttcgtt cgaagccact gtatctttgt
     3301 ctttactatt tgttgaacat gccccgaata taaaaagagc aatcaaaatc cccagacttc
     3361 ccagtactgc tttccatttt ctcatcataa ctttctctcc ttctttctta aaaatttttg
     3421 ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa caatacttga
     3481 tcctgctgct atgttaaaac tataaccaat gaacaaacct agcaatgatg agcacgctcc
     3541 gactgtcgcc gaaataaata tcatagtttt taaactgttt gcatataaat atgctgtagc
     3601 agctggtgta attaacattg caacaattaa tatagttccc acactttgca tagctgttac
     3661 tgctactaaa gttaatagaa tcattaatag ataatgataa aaactgacat tcatccccat
     3721 tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa atagaacaac
     3781 tgtaaccaac actaaaacag aaacgccaat tgtaatccac ttatcaatat cttgtacagc
     3841 taaaatattt ccaaataaga tatgaaataa atccgttgag ctatttgcaa ctcctataag
     3901 gataactcct aatgctaaaa aagaactgaa agtaatacca attgctgtat ctccttttat
     3961 tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa atacaatcgc
     4021 accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca aaacagcatg
     4081 agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac ctactgttcc
     4141 agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt gaaagtcata
     4201 cagaccatta ataaaatttt gaagcatatt attccccttg tatattagag ttagggtttt
     4261 aaccttccgg ttttgattgg ataaccctat gaaccatcgt tattttaatt tggtattaac
     4321 tcatttcttt tgagataata tatttattgg attgagaggc taagccgttc ccccctctcc
     4381 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
     4441 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
     4501 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
     4561 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
     4621 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
     4681 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
     4741 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
     4801 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
     4861 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
     4921 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
     4981 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
     5041 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
     5101 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
     5161 ttttataatt tagatttttt tctgttgttg aagattagga tgtcaaaatt atttgaaact
     5221 actcgtatag taaatagtaa gtaattttca aaacacgaag aaaaacaaaa agaaaaaagg
     5281 aaacgccgaa gcatttcctt tcagtgattt aatgagaaca acacgatttg acttagagtc
     5341 tcaaaaagtc gaataggtac actcatttaa gtgtctccat tcgacttttt ggggatgagc
     5401 tggctattga ggtccgctta ccctattgaa taaatacaag attgggtatt tctaataagt
     5461 ccgcccttca tattggggtg atcggggcga acatgccatc ttttcatata ccacctcctc
     5521 gacactcaat aatcaaagat tctcgttaat cttcttcgat gcttgcaatg atgttttcaa
     5581 tattcatata acgaaaatct tcttgtttga tataaacgat ctgagcggta catttttcct
     5641 ttttgatgat ttcttttact ttttcctcag aacgtgcgac ctgcggcgtt aaaagtaaaa
     5701 cgtcatagtc tttataattc agtttttctt caacgtttcg gatcgttgca taatctatct
     5761 catacgatag atttttggct ctgatcgttt caagcatttt tgatacgagc agcgacgcac
     5821 tgattccggt ctcgcaaata atcaatagat ccttatgcat aataccctcc tgtttttagc
     5881 ctgcaaagcc aattggtgaa aatgccacat aagttaaaat aacaatgact accaatgcta
     5941 tagcagtcac cttggtgtat ttccaaggct cgatatcgac ctttgtatcg aatgagttca
     6001 acacaaattc tccatcaggt ttcaatttgg aaaccaccat caaaatgata atgtccagaa
     6061 cgaataatgc cgtcaaaaca taaaggaaat ggatattttg gaaaagcatt tttgatagtg
     6121 catacaagac gatatgcacg gcaatcgtcg tttttgcccc aaaagcagtt gctttttttg
     6181 aataaaaggc aaagagaatg atcacgagca gcggcatgtt gtatagtcca ttgaattctt
     6241 gaacgaactg atacaatcca gccggtgcaa aagaaatgaa tggcgctacg ataatggaga
     6301 taatccccac aacgaccgtt actgttttac cagcacgagc gatttgctta tcatttgctt
     6361 tcttattgat aagcggttta taaaaatcca gtgaaaacaa ggtcgatgtc gcgttcaaag
     6421 ctcccgcaaa tgatgataag atcgcgccaa agataacagc ggcgaaaata ccaaagatca
     6481 ttttaggcaa tacttcactg actaaaaaag gatacgcgtt atctggattt gacattaaac
     6541 tatcgccaaa aatattgcga gcgacgatcc ctggaaagac aaggaataac gcacctaaga
     6601 ttttgaagac gccgactagg aaagcgccct tttgtgcctc agctaaattc ttgccggaca
     6661 atgctttttg gacgatcatt tgattggtac accagaaata cagattgtta aacagcatcc
     6721 ctgtaaatag tgtcggccat ggaacaacag ccgagtcgat cgatcccaca gagttaagca
     6781 gccatggcgt gtttaccaca acgtgttcga tcccgccaac aaagctgccg tcaccaagct
     6841 tcattaagcc taaaattggg accagcagac cacaagccag cagaccgact ccataaatgg
     6901 agtcactatt ggcacttaac gacaagccgc cgatcagcag atagaggatg ccgacaatac
     6961 caattgccat cgctacaatt atgatcgcga cgatcggctg gacgcccagc agctcatcaa
     7021 tatggaagat cttattgaac accagcgatc cagaataaag aaccacgggt aagaaagaag
     7081 tcatataggt cacaataaat aagatcgaaa taattcgttt ggtaaccgta tcataacgaa
     7141 tctcgatgaa atctgagatt gtattgatgc cgtatttaaa atattttggc aagaatatca
     7201 cagccaaagc tacgatcgca atcgtagcag ttacttccca ggccattact tccatccctg
     7261 catgataact ttgaccattt tgtccgacta tttgttcggt ggacaggttc gtcatgatga
     7321 tcgtgccggc gattggcaaa gcagttaagc tccgccctcc catgaagaac ccttctgatc
     7381 cagaagtatc gacacttctg gatctagaat aggcaaataa ccatacacag atgacaatca
     7441 ctaggaaact gattatagaa aaaagattca tcgtctagct ccttttcaat agattaaaga
     7501 ccggtgacct ctcgaatgta gcttctggct ttgactgcat attcaaatgg attggcttta
     7561 gccgggtcct gttctgcttc aactacgatc cagccttcat agtcgctggc atggattgct
     7621 gtccagattg gagtgaaatc aatcatccca tcaccaggaa ccgtgaagac gcctttcttc
     7681 acgccgttta agaagctgtc tttttccttt tctacttctt cagccgtttc ttgacgaata
     7741 tctttgaaat ggatgtgttt gatacgatct tgatgacggt tgtaaaccgc gattggattt
     7801 tcaccagaaa agaccaaatg tcccgtatcg aacaataatt taactttgtc tggatcagtc
     7861 atttccatta aacgatcgat ttcttccgtc gtttgaacgc cggtacccat gtgatgatgg
     7921 taaacgatct ccatatcctt ttcgtgggcc aaatccccta atttttctaa tccttccgcc
     7981 agttttttcc actcttcatc agtgaaaact ggtttatcat caaagactgg cgctgtcata
     8041 atcccttgga tactatgacc ctgctcagct actacgatga ctttagcgcc catcgcatgt
     8101 aagaaatccc gatgtgcttt gaaggcttcg gcagtttctt caaaaggttt agttgtcaga
     8161 aacgcactga accatgcact ggcaaccttt aagccgcgaa tattcaaata ttgttccaac
     8221 acctctggat cttttggata tttgttgcca atctctgttc cggtgaaccc agcaagggcc
     8281 atttcactga tacattgttc aaaggtattt tcactaccaa gctcgggcat atcatcattg
     8341 gtccacgcga ttggtgcgat ggctaattga atttgtttgg tcatattctc acactcctgc
     8401 ttattttttt agttgtacta aactcttcgt atcgaatgat tcttgacaag ccaacgcgat
     8461 cttagttgat tgcaatccat cttctgcatt aaccgctggt tgacgttttt cttgaataca
     8521 tgagacaaat tcttgggctt cacgaatgaa ggcttctctg aatcgttctg ggaagttttg
     8581 tgaacatgga cggatgatcc cttgatcgtt catgatcgtc acaagatttt tctcaggcgc
     8641 ttgcgctacc cgcagcattc ctttggttcc catgatctct gtttcgacat gatagccatg
     8701 ggctgcgttt ctgccggcaa ctagtaaagc tattgtttta tcgctcaggc tcatcattgc
     8761 ggctccagtt tcaagctcac cagcctgatc caattctgga tacgccgcat ttttcccaat
     8821 cgcccacact tgatcgactt cttggccggt aaaccagcga accaaatcaa tatcatggat
     8881 cgccatatct aagaacaagc cgccgctgtc gttattcatg gcaaatttga caaagctttc
     8941 caagccttcg ctcggatcga tcccataaca acgaatcagt gtgatttcgc cgatttcgcc
     9001 atcttctacc attttcttcg catactgata cgactcatca tagcggcgca tgaagcctaa
     9061 catgaagacc tgttcaggat gctcttcgat gactttttgg gtctttttaa tatcttcaac
     9121 ttcaaggcct aatggttttt cagtaaagac atgcttgcca ttttccatcg ccaagcggac
     9181 ttggtcacag tgaaagcctg atggtgaaac aataaagact gcatcgatca cggaactttt
     9241 gaccatttct tcatagctgg aaaaagtatg cttgacgcct aatttcttct gtgcgaatgc
     9301 taattctgtt tcaactaaac tgcacgcagc cgtaagctca caacaactga cacctgttgc
     9361 taaattttgt gcatgtactt ttcctaatct tcctaaaccg acgatgccta ctctaatgtt
     9421 gttcatgaaa tacctccgtt ttctgagaat tactcattgt atagtgctgg tcgttcatcc
     9481 atttgaatca ttacttttgt ttttgtttca cgagactcat gacaagccat catggtgatc
     9541 gctgctacat acccatccca agcggtagga ccgacaaccg catcattttt gatcgaatcg
     9601 atccacagct tgaattcata atcataggca tccaaaaagc gggtctgcca atctggtgaa
     9661 acaggtgtgt aatttttttc ttccgattta attcgtgtat aggctgggtc cgtcattcct
     9721 acttctgccg tttcgaaaac cacatcacat ttaatatcgt agccaaagcg gcaattcaca
     9781 aagatttcca aatcaatcgt tacaccggat tttgtttgaa agatcagcag ctgcggatca
     9841 tgcaaatcgg catgggagtt gctggttttt ttcggcaaga tcatttgaac agattcataa
     9901 tcttcattta gcaaccagcg aagcacatca atctcatgaa tcgcggtgtt gctgatcgcc
     9961 atcggtgttg tgtagttttc atctgcgcta ggatttctat gggcacaatg catcattaat
    10021 gcttctccgt attgcttcga ttcgattgct tctttcagtt caatgtagcc gcggtcatag
    10081 cggcgcatga agccaacctg aaccaatttc ttcccataag caacttccgc atcaacgatc
    10141 cggcggcagc ctgctgcatc catcgctaat ggcttttcgc agaaaacata tttcccatgc
    10201 ttgattgctt ctaacacata ctgctcatgg gtcgggtccc aagaagtcac aacaacgaca
    10261 tccacatctt cagaagcgat caatgcttct ccggtttcaa agaatttagc atcgacacgt
    10321 ttggcaacct ctttagcagc ttttacattg atatctgaga ctgctgtcac ataagcccct
    10381 tgtgttttct gattgatcct gtcgatatgc tcgatgccga ttgcacctgt tccgataacg
    10441 cctactttta ctctcaatta cttatccccc tgttctgttg tttaagcaca catagtatgc
    10501 gcttacaaaa ttattcctgt gtagctttta ttcttaaaag gtagagccct ctctttgctc
    10561 cgccttttac tacccataag cttgtacgta taattcctta atttgactat gactaggcac
    10621 ctttttatta ttttgcggac ttccgctggt caacgcatcc gcggccattt tgtctaaggc
    10681 tgccataaac gcttgctgat caataccata atctcgaatc gttgggatcg acagctgctc
    10741 acagagctct tggatttttt taatcagata atccgcagcc tcttccagcg ataaatcttc
    10801 ggctgccgga taaataaatc tggcgatcgt cgctaactca gtctgaatag catcctttga
    10861 gaattcaaaa aagaccggca ataacatcgc atttgatatc ccatgaggaa tgtgaaaaag
    10921 tgcccccacc ggtcgactca ttccatgaat caatgtgact gacgcattcg aaaaggcaat
    10981 ccccgcctcc gttgaagcta agctcatttg ttctctggct tcagtgtcgt cgctattttc
    11041 atatgcctga gaaagcgtgt tcaaaatttt cttcatcgct tccagtgaaa atagttgcgt
    11101 tacttcattg gctttgactg aaacataaga ttccaaagcg tgacatagcg catcgactcc
    11161 cgttgccgcg gttacttttg gtggtacagt tttcgtaaaa tcagagtcaa cgattgagat
    11221 ctgtggcatg aaaacgagat ccttcatcat cattttgact gctgtttttg tattggtaat
    11281 aactgccaca tcggttactt ccgaaccagt tccagcagtt gttgacactg caatcagcgg
    11341 aatgcccgct tgcagctgat caaaatttct tgcatgaatc gcttcgagac ttaattgatt
    11401 agcagccaaa acggctacca ccttggctgt atccagacag ctgccgccac caaaagcaat
    11461 gatatgatca cattgatgct tctgaaaaag cttcagcgca tcataaacat tttcttgcgt
    11521 cggttcggct tgaatgtctg tatactccgc taattgatca tctgtttttc ccaacagctc
    11581 cttgatcttt tgaagatgcc cggtctgttc cataaccgta tcactaatga ttaacggctt
    11641 gttgcctagt tgttctgatt cttccataag aaattggatt gttccgccgc caattcttaa
    11701 attggcaggc attttcagtt gaatattcac agaaaaaacc tctctctggc ttatagccta
    11761 taacagaatc ctcgaagctc tttaacagag cttcgaagca aatctttaaa tctcactttg
    11821 cgcataacgt gctgtaacaa ctttccgatg ggtataaaac tcaacactgt ctttgccatt
    11881 ggcatgcaaa tcaccaaaga aagagtgctt ccagcctgaa aaggcaaacc aagccatcgg
    11941 tgcaggcacc ccaagattga cccccagcat cccagcatca acattttctc tgaagtagcg
    12001 aatcgctgat gcgttgttgg tatacagaca cgaaccattc gcaaattcag attcattcgt
    12061 cagctcaacg gctgccatca aatcttttac ccgcacgatt gatgcgaccg gggcaaaaat
    12121 ctcatcctgc cagatcttca tatccatcgt gacattatca aaaatcgttg gtccaacaaa
    12181 atagcctttt tctggaatgt tttcgcgacc atctagtacc agtttcgctc cttcatcgac
    12241 ccctttttgg atatagtcaa aggtccgctg ctgtgcatct tttcgaatca ctggtcctaa
    12301 aaagacccct tcgtccaagc cattgccgat cttgatcgct tttgccgcag ccttaaattt
    12361 ttcaacaaac tgatccgcga tttcctcttc caaaacaatg acggaggctg ccatgcagcg
    12421 ttcaccagca gaaccaaaag ctccgccaat aaggtccttg actgcccgat caatattggc
    12481 atctttcagc acaacaacat gatttttcgc tccagtcaac gcttgtaccc gttttagatt
    12541 ttttgttcct tcacggtaaa cgtattcacc gacatttttt gatcctacaa agctgatgcc
    12601 ttttaccagt ggatcttgca gcaatgtgtt gacaacatcg tgggcaccat agacaacatt
    12661 aaaaacaccg ctaggaaagc ctgctttttc aaccagctca acaattttct ccatcaacaa
    12721 tggtgttttt tctgaaggct tcagcaccac cgtatttcct gccgccagag ccatcgggaa
    12781 gatccagaag ggcaccatca ttggaaaatt aaacggtgtg atccctccga ttactccgat
    12841 tgggtaacga taattcgtga tctcaatatc agtcgccgct gttgataaag aatctcccat
    12901 cattaaattg gtgattcccg ctgcgtgctc cacattttcg attcctcgtt gaacttctcc
    12961 cagtgactcg gacaatgatt tcccattttc cagcgtgatc aaacgcgcca attcactttt
    13021 atgatctgtt aaaagctgat gcagcttgaa cagataacgt ccgcgaacag gaacaggaac
    13081 ttttttccaa gtttgaaacg cagcatcggc agctttaact gcttcggctg tctctgcttt
    13141 gctagacaac ggtacattcg ccagcgcttc ttctgtagcc ggattcatta cttcttcaaa
    13201 ctctgttgag cgactagcga cccaacgacc atcaatataa tttcttaaaa atcttgtcat
    13261 tta
//
LOCUS       DAYYAO010000074        12908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000074 DAYYAO010000000
VERSION     DAYYAO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.077"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACHARI_002111"
     CDS             131..3658
                     /locus_tag="ACHARI_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8443133.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACHARI_002112"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACHARI_002112"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8443134.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACHARI_002113"
     CDS             7446..7787
                     /locus_tag="ACHARI_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8443135.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACHARI_002114"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACHARI_002114"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8443136.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACHARI_002115"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACHARI_002115"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8443137.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACHARI_002116"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACHARI_002116"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8443138.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4385 a         2244 c         2739 g         3540 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgac tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaac
//
LOCUS       DAYYAO010000075        12815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000075 DAYYAO010000000
VERSION     DAYYAO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.078"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(454..879)
                     /locus_tag="ACHARI_002117"
     CDS             complement(454..879)
                     /locus_tag="ACHARI_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443139.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1253..2224
                     /locus_tag="ACHARI_002118"
     CDS             1253..2224
                     /locus_tag="ACHARI_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8443140.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2256..3653
                     /locus_tag="ACHARI_002119"
     CDS             2256..3653
                     /locus_tag="ACHARI_002119"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8443141.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3812..4435
                     /locus_tag="ACHARI_002120"
     CDS             3812..4435
                     /locus_tag="ACHARI_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443142.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            5184..7277
                     /locus_tag="ACHARI_002121"
     CDS             5184..7277
                     /locus_tag="ACHARI_002121"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8443143.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            7437..8303
                     /locus_tag="ACHARI_002122"
     CDS             7437..8303
                     /locus_tag="ACHARI_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8443144.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(8325..8762)
                     /gene="ndk"
                     /locus_tag="ACHARI_002123"
     CDS             complement(8325..8762)
                     /gene="ndk"
                     /locus_tag="ACHARI_002123"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8443145.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     regulatory      8935..9109
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            9451..12099
                     /gene="mgtA"
                     /locus_tag="ACHARI_002124"
     CDS             9451..12099
                     /gene="mgtA"
                     /locus_tag="ACHARI_002124"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8443146.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     gene            12186..>12815
                     /locus_tag="ACHARI_002125"
     CDS             12186..>12815
                     /locus_tag="ACHARI_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8443147.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDL"
BASE COUNT         4277 a         2153 c         2675 g         3710 t
ORIGIN      
        1 ctacgtgatg tagaataagc ctttctatcg tttggttatt ttatattcaa atatttgaaa
       61 taaataaatc tttactcttt gatttgtaaa ctgaaatgat ccactgtttg ttttactgat
      121 gaagcttgat caaaggaaga agctgagttt gaagttcata aaaatcagcc atagtgagtt
      181 ttgcttggat ttgtttatct tcttctactt tttacataat tttgaacagg ataagattca
      241 aatgttttaa atgaatcttt gactagtttg tgacagttgt cgcatatgct caagcttcct
      301 tggtcattaa attctgtatg gatatttttg ttgttattac atgtacaata atcaggacta
      361 tcgatcatcc atttcgtctc ctttacaatc atcttgcttc tttactatag cagaaaagaa
      421 ggggagaaac aaaagaagat ccaacagacg gcactataga ctgtagatgt tgccatcctc
      481 atccttcaaa cggtaattac cgatttttgt aaatatgact gttgtttctt ttttattttc
      541 ttttcctgaa ataatatatt cgtcatcttt ggtagtgatc ttgatatctg tcagcactgt
      601 tttttcttca gatgattcag aagaatcagt tccttgtgtc tgatacgtgc gtgtccattc
      661 cattttttta tctgtaaatg tcaactgtcc cgcggataaa tccatatctt tacgtacttt
      721 tagcgtatat acactgttgg tggtatctgc tttttccgct tttaagacag aagaatcggt
      781 tgtttcgtcc gaggattggt cagagccgcc aaacaaatca cagccagtca atgtcaaaag
      841 tcccgcagac aaaataaata gtacgattaa tttcttcaaa atacttcccc catttgatag
      901 gataaagaaa ttataccatt ggaatgtaaa acttcaaacg gatggaagag aaaaacgcac
      961 aaaacaccca caaatagagt aaattttaga ataaaatttt aaaaaggcaa aaaatccgcc
     1021 actttcagaa agcgttttaa aaaagtgttg caatctttta aaaaaatgct atattaaaaa
     1081 tgaaccggtt caaatacttg tttaagtata attaatagtg agaggaagtt gctctactga
     1141 agactttagt tatatggata ttgatgacaa gcaacactat gcaaagaatg cggtcatcat
     1201 aatcgaacaa caagaaagag aaatcaaatg taactggcag gagtgagaat gtatggcatc
     1261 gatccgtgat gtggctaaga tggctggtgt ttcagtggga acagtttccc gttacttgaa
     1321 tggtcagcaa ctgaaggaaa agaacatgaa aaaaatagca gaagcgattt cagcgttgga
     1381 ctataaagaa aacatcattg ctaaagggtt gaagaataat cgaagcttct ctattggact
     1441 tttgataaac gggatttcca gccgttttgg gtcggaagtt gtctcaggga tcgaaagagt
     1501 agctgaagaa aatgggtata gcttattatt aagcgggttt gccgatcagc cagaaaaaat
     1561 tgaacaaaaa attgaatacc tgatgaagca tgtgatcgat gggctgattg tcttcttatc
     1621 cgaagaagag tggtctggat ttgaaatgct cacaaaaatg agtattccag ttatcgcatt
     1681 gaattgtcca gaaggaccag caggtattga tacaatatta gtgaatgatc gagaaagtgt
     1741 agcaaaagtg atcgctcatc atggtgaaca aggacataaa aaaatcggat ttattgctgc
     1801 cactcaaaca gactacgtag caagagaacg tttagcagga gccaaagaag cggtccaaaa
     1861 tgatccatcg atccaattag aggtctttac aggagactat tctagaaaaa gcggctatta
     1921 cggggcaaaa gcattattag aaaaaggggc aactgctatt tttgtcagca attacaatat
     1981 gtcgattggt gccatcgaat gtttcaatca agaaggtgtc agaatcggga aggatattgc
     2041 gttcagtcac tatgattatt tagataaaaa cgatctgagt atccttccga aaatcacgat
     2101 cacgccacca acagataaaa tcggggaacg agcagcagaa cagctgttaa aacgaatcaa
     2161 aaaagaagag gaagaaaatg aaaaagtgat tgtgatggaa aatatcatac atggaatcaa
     2221 ctaaaacgaa ctaaaaaaga aaaagaggtg ctttgatgaa aaaatatcaa tttccagcaa
     2281 atttttggtg gggttctgca gcaagtggcc cacaaacaga aggaaggatt tcaggcgatg
     2341 gaaaaggaga gaacattttt gattattggt acaaaaaaga accgcaaaag ttctttgatc
     2401 aagtaggacc tgaaaaaacc tcgcaagttt atacgaagta tcaagaggat gtacagttga
     2461 tgaaacagac tgggcataat tcatttcgaa catctatcca atggagccgt ttgatacctg
     2521 atgaatctgg aacaatcaac gaagaagccg ttcgtttcta tcatcgttat tttgatgagt
     2581 tgattgaaaa cggtatcgaa ccctttgtga atctgtatca tttcgatatg ccgatgtatc
     2641 tgcaagaaaa aggcggatgg ttgaaccgag aaacggttga agcctatgaa aaatatgcca
     2701 aaagttgttt tgaattgttt ggttcgaaag taaaaaaatg gtttactcat aatgaaccaa
     2761 tcgttccagt cgaagcaggt tatttatatc gatggcatta tccagaagag tcagatatga
     2821 aaaaagcgat ccaagttggc tatcatgaag cgttggcttc agcgttagcc atcaaatctt
     2881 accatgaaat ggagcaaggt ggtgttattg gtatcatctt gaatctgaca ccaagctatc
     2941 caagagacga atcaaaccct gaagatgtta aagcagcgca aatagcagat gcgttcttca
     3001 atcgttcatt ccttgatcca gctgtaaaag gcgagtttcc aagtgaattg attgagatag
     3061 taaaagaact tgatctcatg cctgtgatcc atgcagagga tttgacggtt atcaaagaaa
     3121 atacagtaga tcttttaggt atcaattatt accagccacg ccgggtaaaa gcaaaagaaa
     3181 caccaatcga tactaaaaac ggtccaatgc ctgaggatta tttcgacaat tatgaaatgc
     3241 ctggtcgtaa aatgaatcct tatcgcggat gggagatcta tgaacaagga atctatgata
     3301 ttttgatcaa tgtacgagat cattatggaa atatcgactg ctttatttct gaaaacggta
     3361 tgggtgtaga aaacgaagca cgatttatca aagaagatgg catgatccac gatgattatc
     3421 ggatcgaatt tgtccaatct catctgaaat atgtccatca agccattcaa gaaggtgcca
     3481 attgtttagg ctatcatatg tggacatgta tggataactg gtcgtggacc aatgcatata
     3541 agaatcgata tggctttatt tcagtagact tagcacaaga tggaaaaaga acaatcaaaa
     3601 aatcgggtca ttggttcaaa gacgtatcag ataaaaacgg atttgaggct taagaaagaa
     3661 tagttgagag caaatgcgtg aaattctcca ttcttaccat tttgtgaaaa agaagttcaa
     3721 ttatttacga tctttttcaa cttttttgtc tttctatttc attttttgta tataatagaa
     3781 tagtcaaaat taaataggag tggaggaaag catgaggaaa aaaagaccgt ttcttctctt
     3841 ttggttattc ggttttttct tactgcttat tttatccgca gcattttcag gaaaaatgac
     3901 agcagatctt gctcagaaag aaacagaaaa aatcgtagca agcaaagaat cagtagataa
     3961 tttcaaaaaa gaattctcaa cttattggtg ggcggagtat aaaataactg ccaattacga
     4021 aaaaggcgaa ttggacattt atttgccaaa aggaaaagaa gaaaatcaat ttattaatat
     4081 cgatgacgta cttagtgcga tttctctcag acaatatcaa tttaaagaca agcatttgac
     4141 cgttttgctt ttgactcatg atggcgacaa agtagcccgt ttgaaaaatg gaaaagtgat
     4201 caccgcttat tctgaaaaag tagacaaaaa agtggtcgcc gatcgtttag cggaatggaa
     4261 aaagacaaat gaacaagtca agaaaacaac tgtctcttct caaaaaaacg agtcgacagg
     4321 aacaagtcct tcagattctg gcagtggaca aacgataacg ggaagtgaag aaaatcaagc
     4381 ctcaattatt acgaatgagt catcacaagg acaatatcaa tcctatggac aatgaaaaac
     4441 agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc
     4501 ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtgtatc
     4561 aagaggtctt gagcctgccg tttaactctg agccacaagg agcaatttca aaaaatagct
     4621 ccttatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     4681 accaagaggt tgtaaaatta gcgtcctgac ataatctaat accgagggtc agctgattga
     4741 acaccgtgta tcaagaggtc ttgagcctgc cgtttaactc tgagccacaa ggaacaattt
     4801 caaaaaacag cttctcatgt tttataaata ttgtgacttg tggccgagga gttggcagga
     4861 gaacaccgtt taaggaagaa gttgtgaaaa agctgtcctg catcaagaaa taaggagctg
     4921 cgacaagaat tttgtcacag cctctttttt tgtacgaata ccgagaaact ggcttttgaa
     4981 taccgtggag tgaagcggtc accacaagac ttttatctct tcattagttt aatcgcttac
     5041 atcgcagcgg ttttatgctg tgaaaagtgg acggaataat ctttagaaag aataaaaaaa
     5101 tcttaagata acaacttttt taggaaattt tcatttttca ggagcaaaat aagaaaaaat
     5161 gtgaatagag agaagtgata tacgtgacac aacttaaaaa attgtgccaa aatcgtttag
     5221 caatcgtttt gacagctgtt ttctttttct ggctgaagac gatcattgcc tactatgcag
     5281 atttttcatt aggtgtggaa ggtacgatcc agtactttat cttgtggatc aacccaattg
     5341 cgactacctt attgtttttt ggcctttctt tgtatattaa aaagccaaag ccagcgttag
     5401 ctattctttt gatcattgat attttaaata cgcttttgtt gtacttgaat attatttttt
     5461 atcgggaatt cacagatttc attacagtca aatctgtatt aggcttttcg aaagtgtcgc
     5521 aaggattgtc aggaagctcc ttttctttga tgaagcccca tgatgtcatc tactggctgg
     5581 atatcgctgt gttcattggg ctactggttt ggttgaaggt caagaaaata cctatcaaaa
     5641 gcaaccctgt cggcaaaccg atggctatcg ctgttacctg tctttcaggg cttatttttt
     5701 caggaaatct agcgctgagc gaagccaacc gtccacaatt gctgcagcga acatttgacc
     5761 gttcatatat tgtcaaatat ctaggaatcg atgcgtatac tatttatgac ggaatcaaaa
     5821 caggaatgac tagcagtgtt cgcgcgcatg caagcagtaa tgggattgat gaagttttag
     5881 attatacaaa gaaacattat gcagagccaa atcccgaaac atttgggatc gctaaaggga
     5941 aaaatgtgat cgttctccat ttagaaagtt tccagcagtt tttgattaat atgaaggtag
     6001 acggacaaga agtcacaccg tttttaaata gtattttcca gaatcaagca acgatcagct
     6061 ttgacaattt ctttcatgaa gtaggacagg gaaagacaag tgatgctgaa aatatgttgg
     6121 aaactggcac atttggttta cctcaaggct cattattcac agaacttggg tcagacaatg
     6181 tctttcaagc cgcaccagcg atccttggac aaaagcaagg ctatacaagc gctgttttcc
     6241 atgggaatgt agctagtttt tggaatagag atcatgtata taaaaactta ggttatgaca
     6301 atttctttga tcgatcttat tttgatgaat ccgacgaaac gttaggttat gggatattag
     6361 ataaagattt attcagagaa tctgcccaat atcttgagca tctacaacag ccgttttaca
     6421 cgaaatttct ttccgtaacc aaccacacgc cttattatac ggatgacaag aatttcgact
     6481 ttccatcttt gaatactggc aatagcacag tagataatta tgttcgaacg gcacattatc
     6541 tggatcaatc attggaacaa ttttttacgt atttgaaaaa atctggtatt taccaaaatt
     6601 caatctttgt gatttacggg gatcattttg ggatctcgaa tacagataat aaagatttag
     6661 caagcgctct tggtaaagat ccagatacat gggatgaatt tgacaacgca cagatgcaac
     6721 gagtgccatt gatgatccat atgcctggtt atactaaagg gacagtcaat catgaatacg
     6781 gtggagaaat cgatgttttg ccgacattac tacatctatt gggaatcgat gataaagatt
     6841 atcttcattt tggtacagac ttattatctt ctcagcatga tcaagtagtt gcttttcgaa
     6901 atgggaactt tgtgacaccc aaatacaccg tcctaggagg aaaagcttac aataatcaga
     6961 caggagaaat catcgatacg aaagcagcag gaatcgatga ggaaatctcc aaagatcagg
     7021 aaaaagtcaa gactgcgtta agtctttcag ataagctgaa tcaggaaaat ctcttgcggt
     7081 tttatgtgcc aaacggattt gagacgattg atccgaaaaa atatgactat aaaaatgaag
     7141 cggaacgtaa tgaagcaatc gaaaagcaaa aaggcgaaca ttcgacaagt gtcttttcaa
     7201 aaaatggtaa taaaacaaca accaacttat atccagtaac aagaacagac gttgaacagc
     7261 aaaaaaataa tcaataaaca atcaaaccaa ccttcagtag ttagacaaca ttctaatgct
     7321 tgaaggttgg tattttttat gaaaaaaaca tttttttatt tttttcgtgt atttttcctt
     7381 tttaacgtat ctatttattg aatgtggaaa aatttaaatt aaaaaggaga actattatgt
     7441 atgatggtga attgatcaag aaacttcgag tcaacaagaa gttgacacag tcccaattag
     7501 cggaaggaat ctgttttaag acttctctag tcgggattga gacaaattca gtgaaaaaaa
     7561 tgtcatttgt aacattgaga tcatttttag agcgaatgaa catgacactt gcagaatatg
     7621 aatggatgcg aaaccagctg gatgaaccaa taaagttaaa gaaaaaaaga cgaatgctag
     7681 atcgggtaca agaagacagc tttgatcctt ataaagagat cagcaataac cgtaggcatt
     7741 ttaagaaaac agaagatctg tattatttgg ttttgaatct tcacatggtc gaaaaaacag
     7801 aagtatctga gacagaattt gaatttttac gttatgaatg ccgaatcatt gaagaatact
     7861 tgatgcagat accagactat ggtcaatttg aactatctat tctagctgat tttccttgga
     7921 tattcagtga tcggttcatt gagaacaatt atttgaaaat caaaaaaaga atgcgacgat
     7981 tagcacagtt gaacaatcat gagcagtatc tctttacttt tttaatgaac ctgacttcgt
     8041 tttatataga aaatggtcgg ttgaaaaagg cacgtagtgt gaatgaagat atgaaacgga
     8101 gcttagcaca taaagaaaga agtacagtga tctatgaaac attgatggca gattattatc
     8161 aaagattgat ttctgctgct ttaggggaag aaaagacgaa agactaccaa gaattcttca
     8221 cagtgctcga atatatgtta ggaaagaatc aacggaggtc cttagagaaa aaactgctgg
     8281 ccattgctga acccagcaac tagaccagca gtttagaatt tgtatcactt cgccgctttt
     8341 tcagacggtt tgtttatatc gaatggcggg aaaaagcggg cgatctcttt taccgcagca
     8401 tcacgggaat cagaagcgtg aatgatgttt tcagtccccg gcagcgcgta gtctcctcgg
     8461 atcgtacctg gaacagcatc ggcagctttt gttgctccaa ccatcttacg gacgatatcg
     8521 atcacttcct cgccgaccag cacaagataa acgacaggac cggaagtcat gtaatcgatc
     8581 agttcatcaa aaaaactgcg ctcagtcaag tgctgataat gttcttttgc taattcagga
     8641 gtcatagttt caaacttcat ttcagcaatg gccagtccct ttgcttcaaa acgttggatg
     8701 atgcttccga ctaaatggcg tctcacgccg tctggcttga taataacaag tgttctttcc
     8761 ataacagcac ctcgtttcct actatatttc tattatagcg ggaggtggaa aatgagacaa
     8821 ataaaaccgc gtgagacagg aatataacgt gtgtatttta aaaaaagtat gaaaaacatg
     8881 gaaaattgat ttgacatcct cttttttagt tgttatatta acgaaggaaa accgaataag
     8941 atttgttagg tgaggctcct atacaaacat aggctactgc ccaaaaacgt cgagagacgc
     9001 caatgggtag aacaggaatt gtcgagataa ggcttttcat aaggtagcta aaagcaggta
     9061 tctgcttttt acgttgtaca gtgtcaaaac tcaacgatga gatctgagaa gcttttttta
     9121 atatgctttt tcaaaccttt cgctgagtat ttggcgagag gtttttttgt tgccgcaaat
     9181 ccccatcggt gaaaaataaa aaggaggtgg tctaattgga aaaataacta ttcgaaaaat
     9241 gattggaagt catgaattaa aattacttag taaaggagat gagaacaaat ggatgacagt
     9301 cattcgaaaa acaaggatct agaggaaagg ccagggctat tatctatctg ttctcagccc
     9361 ctttcagttt tttctctatc gatccgatga atagctaaag accaaaagca gcgagaacag
     9421 ctgtcttttg gacacaaagg agagattgag atggaaaaaa agaaagatac acaaaaaatc
     9481 tatgcagctt ttgctggaaa aacgaaacaa gaattatttg aactattcca tgcatcagca
     9541 aatggattga cagaagaaca aatcgaacgt tcaagagaaa attacggtga aaatgcgatt
     9601 tcatatggca aaaaaacacc attcattgta gaaatattga aagcatacat cacgccattt
     9661 acattagtgt tgatcgcatt aggaattatt tcattcatca cggaatatgt attagcagct
     9721 cctggagata aagatctttt cggtgtagcc attatcttca ctatggttat cgtcagcggg
     9781 acaatgacgt tgattcagtc cgtacgttcc aatcaagcgg ctgaaaaact gaaatcactt
     9841 gtgaaagtaa cggcagctgt aaaacgtaat ggaaagtaca cagagattcc tatggaagaa
     9901 atcgtctgtg gagatcttgt tcggctttca gcaggggata tgataccagc ggatatgcgt
     9961 ttattgtccg caaaggattt attcatttcc caagcagcat tgacagggga aagctatcca
    10021 gtagaaaagc atgcgaaagt atgtgaagac gatcagcaaa gcgaaacaag ttacgaaaat
    10081 cttgtcttta tgggaagtaa tgtcatcagt ggaagtgcgg aaggaatcat tgtttctgtt
    10141 ggaaatgaaa cactatttgg tcatgtagca gaaacgctat ctgaaaaacc aattcaatca
    10201 agctttgaat taggcatcca taaaacatcc atgctactga tcaaatttat gtcattgatg
    10261 gctccagcag ttatcgtcat caatgggctt acaaaagggg attggctaga agcttttctt
    10321 tttggactat ctgtagcagt tggtctaaca cctgaaatgc ttccaatgat cgttacgacg
    10381 aatttggtca aaggagcaag tgtgatggcc aaaaaaggaa cagtgatcaa aaatttgaat
    10441 gccatccaga actttggtgc aatcgatgtg ttatgtacgg ataaaacggg gacattgaca
    10501 caagataaaa tcattcttga atatcatatg gattgtgaag gaaatgaaga tgatcgtgtt
    10561 ttgcgccatg cgtatctaaa tagctactat caaacagggc tgaaaaattt gttagatgtg
    10621 gctatcattg atgaagcaac agaaacttta gatacagata agatcaatta tcaaaaagtg
    10681 gatgaaattc cttttgattt tgaacgtcgt cgtatgagtg ttgtggttgc tgatccagct
    10741 ggtaaaacac aaatgatcac aaaaggtgcg attgaagaaa tgctgaacat ttctaaattt
    10801 attgatcttg gcggtactgt tacaccatta acaaaagaac gaaaagaagc cgtacttgca
    10861 aaagtacagt cacttaatga agatggctta cgagtgattg gtgtcgcaca aaagaccaat
    10921 ccaagcgtcg ttggagaatt ttcagtcaaa gatgagtcag acatggtttt gattggttat
    10981 cttgctttcc ttgatccacc aaaagaaaca acgaaacaag cgttgaaagc tctgaaagaa
    11041 catggtgtag ctgtaaaagt tctgactggg gacaacgaat tagttacacg ttcagtgtgc
    11101 cgtcaagtag gtctagagat caatgaattg gttaccggtg agaaaatctc ggaaatgagt
    11161 gatcatgaat tagcacaagt agctgaagac catgaagtat tcgtaaaatt gaatcctcag
    11221 caaaaagcac gtttgacgac agctttacgt aaaaatggtc atactgtcgg atttttagga
    11281 gatggtatca atgacgctcc tgcaatgaaa gtggctgata tcggtatttc tgtagataca
    11341 gctgtggata ttgcgaaaga atctgcagac gtgatccttt tagaaaaaga tttgacgatt
    11401 ttggaaagag gccttctttc tggacgagaa acttttggga atatcatgaa atatatcaag
    11461 gctacagcaa gttctaattt tggcaatatg ttttccgtgc tgattgcaag tacgttcctg
    11521 ccatttttac caatgctgcc tttacaactt ttgtttttga atctgatcta tgatgtttcc
    11581 tgcatttcct tgccatggga tcggatggat aaagaatatt tggacgagcc aaaaaaatgg
    11641 aatgcatcta gtattgggga attcatggtt tactttggtc caacaagttc gatatttgat
    11701 attactactt acttgttgat gtattttgtt atttgtcctg ctgtagtggg cggaagtttc
    11761 catacattgg atagttcaca gcgagtgatt ttcatcgcat tattccatgc aggatggttt
    11821 gtcgaatcac tatggacaca aaccttagtg ctccatgcac tgcgtacacc gaaagtacca
    11881 tttatccaaa gcaacgcaac atttgccatg tttgttatca ctacattagg aattattgtc
    11941 ggttctgtcc ttccttttac aagttttggt gctgaattag gcttgatgcc acttcctggc
    12001 aattactggt tatggttatt tgctacagtc atcgcttatc tggcgcttgt gacagctgtg
    12061 aagaaaatct atatccgtcg attccaagaa ctcttataaa cgagaaggct ggttctttgc
    12121 ttctgttctt tttggaccag tcgtgctaaa ctatgtgtat tgttttggaa aggattaatg
    12181 tatagatgaa aggtgcaata tttgatttag atggtttgtt agttgacaca gaaaaaacgt
    12241 atcgtgatgg ctggctttgg ggatttcagc aatttgatat ccatatccca aaagaaatcg
    12301 ttgacgcttg gggcggaaaa aactggaaac aaagttttga tattttagtg aaagcagcag
    12361 gtagccctga aaaagtacag gaagtccgag cgaagcgtga agaatatttt tatcaacagc
    12421 tgacaagcgg agccatccac ttaaaaccat atgcaaaaga aatattggca gacttaaaag
    12481 aacgtcatat gaaactaggc attgctacga ccactgttac gaaaagagca acagatatct
    12541 tggatcactt taaattagct gattacttgg acacgtttac attcggagac gaagtgtcgg
    12601 agaacaagcc ttcaccgatt ccttatctga cagctttgga acgaacagga ttagaagcta
    12661 gttcagcctt tgcagtggaa gattctttag taggagcaac agctgcttca cgagcaggat
    12721 taggcgttgt tttgatacct gattccagtt ttgtccgaaa ttataccgtc gaagaaaagg
    12781 aaaaattgaa acttcttgcg gaaggaaaag atctt
//
LOCUS       DAYYAO010000076        12740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000076 DAYYAO010000000
VERSION     DAYYAO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.079"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..542
                     /locus_tag="ACHARI_002126"
     CDS             99..542
                     /locus_tag="ACHARI_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8443148.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="ACHARI_002127"
     CDS             complement(548..1732)
                     /locus_tag="ACHARI_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8443149.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="ACHARI_002128"
     CDS             2000..2698
                     /locus_tag="ACHARI_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8443150.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="ACHARI_002129"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="ACHARI_002129"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8443151.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="ACHARI_002130"
     CDS             complement(3508..4041)
                     /locus_tag="ACHARI_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443152.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="ACHARI_002131"
     CDS             complement(4338..6719)
                     /locus_tag="ACHARI_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8443153.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="ACHARI_002132"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="ACHARI_002132"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8443154.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="ACHARI_002133"
     CDS             7420..7959
                     /locus_tag="ACHARI_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8443155.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="ACHARI_002134"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="ACHARI_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8443156.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="ACHARI_002135"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHARI_002135"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="ACHARI_002136"
     CDS             9066..10232
                     /locus_tag="ACHARI_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8443157.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="ACHARI_002137"
     CDS             10278..11774
                     /locus_tag="ACHARI_002137"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8443158.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="ACHARI_002138"
     CDS             11855..12538
                     /locus_tag="ACHARI_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8443159.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12547..>12740
                     /locus_tag="ACHARI_002139"
     CDS             12547..>12740
                     /locus_tag="ACHARI_002139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443160.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4273 a         2230 c         2630 g         3607 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    12661 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
    12721 gcaggagaac accgtttatt
//
LOCUS       DAYYAO010000077        12735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000077 DAYYAO010000000
VERSION     DAYYAO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.080"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHARI_002140"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHARI_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8443161.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHARI_002141"
     CDS             complement(323..892)
                     /locus_tag="ACHARI_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8443162.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHARI_002142"
     CDS             1078..1980
                     /locus_tag="ACHARI_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8443163.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHARI_002143"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHARI_002143"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8443164.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHARI_002144"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHARI_002144"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8443165.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHARI_002145"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHARI_002145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8443166.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACHARI_002146"
     CDS             8548..8733
                     /locus_tag="ACHARI_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8443167.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACHARI_002147"
     CDS             complement(8795..9991)
                     /locus_tag="ACHARI_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8443168.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACHARI_002148"
     CDS             complement(10161..11597)
                     /locus_tag="ACHARI_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8443169.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACHARI_002149"
     CDS             11890..12699
                     /locus_tag="ACHARI_002149"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8443170.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2672 c         2250 g         4011 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtctaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgaca
//
LOCUS       DAYYAO010000078        12528 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000078 DAYYAO010000000
VERSION     DAYYAO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.081"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..763
                     /locus_tag="ACHARI_002150"
     CDS             26..763
                     /locus_tag="ACHARI_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8443171.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            777..1592
                     /locus_tag="ACHARI_002151"
     CDS             777..1592
                     /locus_tag="ACHARI_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8443172.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1605..2252
                     /locus_tag="ACHARI_002152"
     CDS             1605..2252
                     /locus_tag="ACHARI_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8443173.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2268..2924
                     /locus_tag="ACHARI_002153"
     CDS             2268..2924
                     /locus_tag="ACHARI_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8443174.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3086..3907
                     /gene="racE"
                     /locus_tag="ACHARI_002154"
     CDS             3086..3907
                     /gene="racE"
                     /locus_tag="ACHARI_002154"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8443175.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3910..5265
                     /gene="rph"
                     /locus_tag="ACHARI_002155"
     CDS             3910..5265
                     /gene="rph"
                     /locus_tag="ACHARI_002155"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8443176.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5266..5784
                     /locus_tag="ACHARI_002156"
     CDS             5266..5784
                     /locus_tag="ACHARI_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8443177.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5891..6391
                     /gene="cbpB"
                     /locus_tag="ACHARI_002157"
     CDS             5891..6391
                     /gene="cbpB"
                     /locus_tag="ACHARI_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8443178.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6671..7591)
                     /locus_tag="ACHARI_002158"
     CDS             complement(6671..7591)
                     /locus_tag="ACHARI_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8443179.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7717..8769
                     /locus_tag="ACHARI_002159"
     CDS             7717..8769
                     /locus_tag="ACHARI_002159"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8443180.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(8879..9634)
                     /gene="fabI"
                     /locus_tag="ACHARI_002160"
     CDS             complement(8879..9634)
                     /gene="fabI"
                     /locus_tag="ACHARI_002160"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8443181.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9854..10294
                     /gene="fabZ"
                     /locus_tag="ACHARI_002161"
     CDS             9854..10294
                     /gene="fabZ"
                     /locus_tag="ACHARI_002161"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8443182.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10409..11170)
                     /locus_tag="ACHARI_002162"
     CDS             complement(10409..11170)
                     /locus_tag="ACHARI_002162"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8443183.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11359..12453
                     /locus_tag="ACHARI_002163"
     CDS             11359..12453
                     /locus_tag="ACHARI_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8443184.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4036 a         2173 c         2642 g         3677 t
ORIGIN      
        1 taaataataa tgggagtgta attctatggc aatgattaaa tttgatcacg tggataaata
       61 ttatggcaag ttccacgcat taaaaaatat caatctcgaa ttcgagaaag gagaagttgt
      121 cgttgtcatt ggtccttctg gttcaggaaa gagcacgatg cttcgctgta tcaatggatt
      181 agagacgatc tcttcaggta agctgctgat taatgatact gacttgcatg ataaaaagac
      241 aaaactgaca gaagtccgaa aaaacatcgg catggttttc caacatttca atttgtaccc
      301 aaataaaact gttctggaaa acatcaaatt agcaccaatc aaagtgttga agcaagacca
      361 ggcaactgca gtcaaaaacg cggagaaatt tttgaaaact gtcaatatgt tagataagaa
      421 agattcttat ccttccatgt tatccggggg acaacagcaa cgggtggcaa tcgccagagg
      481 tttagcgatg aatcctgaga tgctgctgtt tgatgaacct acttctgctt tggatccgga
      541 aatgattggt gatgtattag acgttatgaa aaaattggct catgacggta tgtcaatgat
      601 tgtcgtgaca catgaaatgg gctttgccaa agaagtggcg gatcgtgtta tcttcatggc
      661 tgatggagaa gtattggaag acagcagaaa tgtgagagac tttttcgaaa atccaaatga
      721 agtccgagca cagcaattta tcagtaaagt tatcaatcat tgataggagg aaaaagatgc
      781 gcaggttaaa aaaaatacgc ctgattttcc ttttaggcat tgcttttctt gcactagccg
      841 gatgtaaagg aaatgggttg gcaaatgaag atattttaga gcgaagcaaa tctaccaatg
      901 aaatcatctg gggtgtcaag tatgatactc gattatttgg aatgatggat attgagagca
      961 gaactgtaca aggatttgat gtagatattg caaaagcaat cactaaaaag atccttggtg
     1021 acaatggaaa aacagaattt gttgaagtca cttctaaaac tcggattcca ttattgaaaa
     1081 acggaaatat cgatgcaatt attgcaacga tgacgatcac agatgaacga aaaaaacagg
     1141 ttgacttttc agatgtctat ttcgatgctg gccaagcttt gttggtaaag aaaggaagcc
     1201 aaatcaaaag tgtagatgat ttgaatgctt ctacaacggt tctagcagtc aaaggttcta
     1261 catcggctgc gaatatccga cagcatgcac cagatgcaaa aattttagaa ttagaaaact
     1321 atgcagaagc atttactgct ttacaatctg gtcagggaga cgcgatgact acggataatg
     1381 cgattttgtt agggattgca gatgaaaatc cagagtatga gttagttggt ggaacgttta
     1441 ctaatgagcc ttacgggatt gcgatcaaca aagggcaaga aaacttcttg aaagctgtta
     1501 accaagcgct agaggaaatg catgcagatg gcacatacga taaaatctat caaaaatggt
     1561 ttccaaatga aactgaagga aaagtagagt aagggggaga ggcaatgagc gaattgatac
     1621 aaacttatgg accaatcttt gtcgacgggt tcaagatcac attgttttcg agtgtcttag
     1681 cacttgtgtt tagtttgatt atcggaacgt taatggcaat cttccaatta tcaaagaata
     1741 aagtagtgag cgccttagca aaggcatatg tagaattttt tagaaacatt ccattattaa
     1801 tcatcgtgat gtttttttat gttgtcgtac cgatttattg gtttaatcta gatggattcc
     1861 aagcaggtac gattggatta acgatctata catctgcttt tattgctgaa acagtacgtt
     1921 cagggattca aaccgtacca aaaggtcaaa cagaagcagg actatcttct ggttttacct
     1981 attcagaaat catgcgttat attgttttac cgcaagcatt caaaatcgtt atcccgccat
     2041 taggcaacca atttatcaac ctcgtaaaaa actcatctat tttagcaatc gtagctggtt
     2101 tagatttgat gtatcaagga gacttgatcg ctagtgaaac attcaatacc ttcgatacgt
     2161 atatcatcgt tgggttattt tacttagtta tcacgctacc gttatcttac ttaatggttt
     2221 atcttgagaa aaaatgggcg gttcgttcat agaaggaggg gaaatacatg gattttagcg
     2281 gcgctttttc atggatgaat atccgttttt tattggaagg attaaaggtt actgttgaag
     2341 tttcattgtt ttcgatcatt tttagtttct tgatcggtgg tctgacaggt gtgattcgtt
     2401 ttgcgaatat ccctttcttt tccaaaatcc ttggtgtagt gattgatatt atacggaatc
     2461 ttccattatt attgatcatt ttcttcactt actttgcctt acctcaaatc ggcattcaga
     2521 tgaacatctt ctggtccgct gtcgcagcat taacgatttt tgagtcagct atgttgtcgg
     2581 aaattttccg agctggactc aatgctgttc caaaaggaca gatggaagca ggattatcaa
     2641 cgggactgac ttatgtagaa tcgatgcgaa cgatcgtgtt accgcaagca tttaaatcaa
     2701 tgatcccggc aattgtgagc caattgattt cactgatcaa agatacttca ttagcagtaa
     2761 tcatttcttt acctgaactt tctcatcagg cacgtattat ttatggacag aacaccaatt
     2821 atgtgcttcc gatgttcatc atcatgacac ttatgtactt cgtggtatgt tacgcgcttt
     2881 ctctgctttc cggttacttg gaaaagagac ggtacagtta ttaatagatg attcttttag
     2941 cggttgtggc aaactaaagt cacaaccgct tttcctcttt ccattcgaaa aaaagagatt
     3001 tcgattgttt taagcaagta agagcacttg aaaattcacg ctaaaaagta cacaataggg
     3061 aggatagaat ttattgaaaa aaaggatgat acgattgaca gataatcgcc ctatcggatt
     3121 tattgattca ggtgtcggcg gcttgactgt agtaaaagaa gccctgaaac aattaccgaa
     3181 tgaaaatatt ttatttgtag gagacacagc acgctgccca tatggcccta gacccgcgga
     3241 acaggtaatc cagtatactt gggaaatgac ggattatctg gtggagcaag gaatcaagat
     3301 gctggtgatc gcctgcaata ccgcaactgc ggtggcttta gaagaaatca aagctgctct
     3361 ttctattcca gtcatcggtg tgatccttcc cggtactaga gcggcagtaa aaaaaacaca
     3421 aaataaacaa gttggcatta tcggtacgat tggtacggta aaaagtcaag cttatgaaaa
     3481 agcactgaaa gagaaagtac cagaattgac tgtgacaagt cttgcttgtc ccaaatttgt
     3541 ttcagttgtc gaaagtaatg aataccattc atcggtggcg aaaaaaattg tggcagaaac
     3601 attagctcct ttaaccacta aaaaaatcga tacattgatt ttgggatgca cccattatcc
     3661 attattacgc cccatcattc aaaatgtaat gggagaaaat gttcaactga tcgattctgg
     3721 agcagaaaca gtaggtgaag tatctatgct gttagattat ttcaatttga gcaattcacc
     3781 gcaaaatggt cggacattat gccagtttta tacaactggc tctgccaaac ttttcgagga
     3841 aatagctgaa gactggcttg gaatcggaca cttaaatgta gaacatatcg aattgggagg
     3901 aaaataagga tgcgtcatga cgggagaaaa gcccaagagc ttcgcaaact ggagcttcaa
     3961 acaaatgttt tcaaatatcc agaaggatcg gtagtgatcc gttttggtga tacaacagtt
     4021 atttgttctg caacaataga aaattctgtt cccccttttt tgagagaaac aggaacagga
     4081 tgggtgacag cagaatatag tatgctgcct cgagcaacga gtacgagaaa tcgtagagaa
     4141 agcagcaaag ggaaactttc tggtagaaca atggaaatcc agcgtcttat tggtcgttcc
     4201 ttgcgtgcag tagtagactt ggaaaaatta ggagaacgta gtatcatcgt agactgcgat
     4261 gtgatccaag cggatggcgg tactagaaca gcaagtatca ctggggcatt tgtggcattg
     4321 aaattagcca ttgaaaaact tttaagagaa aaagaattgt cagaagatcc aataaaagaa
     4381 catcttgctg cagttagcgt aggaatctta ccagatggga cttgcgtcac agatttggat
     4441 taccaagaag attccgcagc acttgtagac atgaatcttg taatgacaga atctggaaaa
     4501 ttcgtggaga ttcaaggaac tggagaagaa gcaacgtttg atggtgaaca gttgaatgaa
     4561 atgctctttt ttggaaaaaa cgcgattgaa gatttgatca aagagcaaaa acatgctttg
     4621 ctaacagagt ttgctcaaaa tgatgagcgg atagaagaaa caaaaacaat catcattgcg
     4681 acaagaaatc caggaaaagc agaagagttt cgaaatatgt tcaaagaagc gggatatcat
     4741 gtaaaaacat tacttgacta tcctgaattg ccagatgtgg aagaaactgg cagcacattt
     4801 gaagaaaatg cccgattaaa agcagaaacg attgcacaat tgcttgatca gccagtctta
     4861 gcagatgatt caggtttgaa agtggatgct ttaggcggta tgccaggaat ctattctgca
     4921 cgttttgcag gggaacaaaa aagtgatgcg gggaataacg ccaaattatt atatgaattg
     4981 acagatgtgc cggatgaaaa aagaacagca caattccatt gtacgttagt attcgcggca
     5041 cccaaaaaag acagccttgt agtagaagcg gaatggcccg gtcgagtagc ccgtatacca
     5101 tctggcgaaa atggctttgg gtatgatcct ttatttatcc cagaaggtaa gaaacaaaca
     5161 gcagcagaat tatcgagcga agaaaaaaac aagatcagtc atcgggctca agcaatgaaa
     5221 aaattgagtg ccgagtggaa acaatggctg gaaggagaga gatagatgcg gtatttagta
     5281 gtaagtgata atcatggaga cagagatatc ctcgttgact tagtagatcg ttatcgttct
     5341 gaagtggata tctttttcca ttgtggtgat tcggaattag aagcttctga cccattatgg
     5401 gatacattcc aagtggttca aggtaattgt gattatggac caggattcga acagaaaaaa
     5461 gtgatccaaa ctggtcagga tacagtattc atgactcacg gtcatttatc gaatgtccga
     5521 tttggtttga cacagttagc aatcgaagct cgagctgcaa ctgccgatat ggtgtttttt
     5581 ggtcataccc atcaaatagg ctgtgagatg gatcagaaaa tcctgttttt gaatcctggt
     5641 agcatttctc agcctagagg cccgatccaa atcccatcat atgctcttat cgaaagttta
     5701 gatgaccaaa tttctgttca atactataac cgtgcccatc aggcagttga agagttggcc
     5761 ttttcttttt cgagagagcg ctgaataaaa aaactctaat ttttaataaa aataagcttt
     5821 attttatgaa ttttgcatcc ggtttcggta aaatagtagt gttgaatgaa atagaaatgg
     5881 aggccatcag atgattggac ctatagtaag agaaatgctg ctggaaaatc aggaaacgtt
     5941 tttgattcct gcagaaaatg tggcaaatgt tatgtatcat catccattgt cccatggttt
     6001 acttgtgtta tcgaaggtcg gttattccaa aattcctgta ttaggaaaag atgatcactt
     6061 tgtaggacta gtaagtttat cagatgtggt caataaaatg attgacttgc aaacaatcag
     6121 catggacccg ttagaaggac taacggttgc tgatgtgaca gaaacagatg ttccgacaat
     6181 tgatgaaaat tgggaactag aagaagtact ccatttgtta gtcgattcgc cttttttacc
     6241 ggttgtcaca gaagatcgga ttttcaaagg aatcattaca agaaaagagc tgcttaaagc
     6301 cgtaaacttc atggtgcatg aattagaacg tcgtaatata ttgtctccga aaatcgaaga
     6361 attaaaagaa caaatcgata tcgtgagcta aagaacgaga aactaaaaga taatcaaaaa
     6421 ataagtagaa aaaacagcgt accagttttg actttatctt gaaaaagagg ctgggacaaa
     6481 agcgtttaac tccaagaaat aagaaggaat tcacgaaaat tgcttttcaa atttttgtga
     6541 atttcagctt atttccgaag gagttgcttc tgcttccgcc gtttatacgt gtttagagcg
     6601 tgggacaaaa gtgttcttta cttttgtccc acgctctttt tagtgagatt cgttgcatcg
     6661 tttgtccaaa ttattttccg acagcaattg gactgttcgg aatcgtgaaa ccagaagctg
     6721 tcaattcttt gacataggag ctcaagaact cttcttttaa ggcatattgt tttccgttca
     6781 atgcgtagca gaccgtacga atcgcaaaat tgctgtttcc aagatccact aatccaaaga
     6841 tagaaggttc agtttggagc tcttcttggt atttttcagc taatgtacga tttactcgat
     6901 caatgatctc atagattttg tcatatcctt cttcaggaac aatccgaata tctaaaagta
     6961 cttgcatatt tgcacgagag agattgctga tcgtggtgat gtttcgattc gggatgaaat
     7021 ggacagtacc atccgttgct ttcagctgaa gggtacggat accaacagat acgacggttc
     7081 cttcaatcgt taagtttgct agcttgatgt agtccccaac atccatctgt tgttccatga
     7141 taataaaaaa tccggtaata acatcactca tgaacccttg ggcaccaaga ccgattgcca
     7201 cacctgcaat tccggcacca gccaaaagtg atccgatcgg cacaccgata gcagataaaa
     7261 ttgcatagat aaaaaagaaa ccaagggtat aatgaaaaat agtatttaaa agactatgta
     7321 aagtctttaa gcggctctcg tttagaatgg cttttttaga atactgatta aaagacttat
     7381 caatcagata ttttcccaca cggtgaagaa tccaaaaaag gacgatcaaa aaaatcagat
     7441 ataaactttt ttggataaag gcagccaaaa tggcgtccca atcaatcccg tcccaaaacc
     7501 gctgaaacgc atttactttg ctgacagctt gatctgtcaa tgtctcgcca gaagagtctg
     7561 tcgtaccggc agttgctaat aatgaaaaca taaaatcctc ttttctaaaa tttgattctt
     7621 tctgtattgt accaagaagt tttcaaaaaa ggaaatagca aaaataacta taatgaagga
     7681 aaaaagctga aaataaaatg acaaataata tggataatga taaaatgtgg agaaaataac
     7741 gaaggtggta agtatatgca aatcgattgg aacaatctag gtttttctta tatcaaaact
     7801 ccttggcgtt ttattgccaa atggaaagac ggacaatggg aagaaggaga attgacagaa
     7861 gataattacc tgacgattca tgaaggctct ccagcgcttc attatggtca gcaatgtttt
     7921 gaaggtctaa aagcttatcg tcgaaaagac ggcaaggtca atctctttcg acctgaacaa
     7981 aatagtcgac ggatgaatca aagtgctaga cgattgctga tgccaatggt ccctgaaaag
     8041 atgttcgttg atgctgtcaa gcaagtggta aaagcaaatg aagattttgt gccgccctat
     8101 ggaacaggcg ctacattata cttgcgccct ttactgattg gggtcggtga aaacattggt
     8161 gtccatccag ctccagaata tttatttgtc gtcttctgta cgccagtagg agcgtatttc
     8221 aaaggtggac taaaaccaac gaattttatt gtatctgatt acgatcgtgc tgctcctaag
     8281 gggactggtg cagcaaaagt cggcggaaat tacgcagcga gtttacttcc aggaacagaa
     8341 gcaaaagaac tggattactc agattgtgtt tatcttgatc cagccacaca tacgaaaatc
     8401 gaagaagtag gtgcagcaaa tttctttgga attacaaaag acggaaaatt catcactccg
     8461 caatcgccct ctatcttacc aagtatcacg aaatattcct tgttgcagtt agctgaagaa
     8521 cgactaggac tgcaagcaat tgaaggagat attttcattg atgatctaga tcaatttgca
     8581 gaagctggtg catgtggtac agcggctgtt atttctccaa tcggtgggat ttatcacaat
     8641 aacaatcttc atgtatttta tagtgaaacg gaagtaggac cagtaacaaa acggttgtat
     8701 gatgaattga caggtattca atttggagac atagaagcac cagaaggctg gattgaagtc
     8761 gtggaataat ttcaaagtaa tgagttgaaa aaaacgtaaa aagaaaaaat caggaagaga
     8821 ctttaaaaag ttttttcctg atttttatat gttttacttt aaagaatgca acaaaagttt
     8881 atgaaagatg gacacctttg tcaacgtaga taatatcacc gatgattccg ctggaaagtg
     8941 ggctgattaa gaaagcacaa gcattaccga cttcttcgat cgtcacacct tgctgatcag
     9001 gcgtacgaga ttcagataac tggatcagtt tttgatagtc tttaacacct gtgacagcta
     9061 atgttttgat tgccccagct gagattccat tgacccgaat tcctttaggc gagaactcag
     9121 ctgccaaata acggatagca gcttcaagag aagctttcgc aatacccatc atgttataat
     9181 tcgggattgc gcgttctgaa cctagataag tcatgctaac gatcccgctg tttggtgcaa
     9241 gaaacggttg tgcgtatttt gatacagcaa ttaaagagta gctgctgata tcttgagcta
     9301 attgatagcc gtctctggaa atatcagaca catttccgga caattcttct ttgttggcgt
     9361 aagcaactgc atggacaagg ccgtggatct ccccagcata ttcaccgatg gcattcattg
     9421 ctttttcgat gctttcgtcg ctagaaacat cacattctac agtgaatgct tcttcgccga
     9481 ccaatttgac taatgatttt ttcattcgtt cattttggta agtatagata atttctgccc
     9541 cttgtttcaa cattgcttca gcacaacccc aagcaatgct ttttttgtta gcgacgccca
     9601 taatgacgat tttttttcct tctagaaatg acataatgac tctcctttgt ttctgataaa
     9661 aatttgatag tcgtaaaaaa atagagaata atagctaaaa taacttcttg atttcttaat
     9721 tattagtcaa aatgttttga ttgtcaaact attttttctt aaattaaaaa aagattgcta
     9781 aatatttagg aatatgatac tttgatagtc aaagtaatga aaatggcaat tgcctgaaag
     9841 aaggagttta attatgtctt acgtattatc tgctactgaa gtaatggaat tgattcctaa
     9901 tcgttatcct atttgttata ttgattatgt agatacttta gaaccaggaa aaaagatcgt
     9961 tgcgacaaaa aatgtaacaa tcaatgaaga ttttttccaa ggtcactttc ctgacaatcc
    10021 agtgatgccg ggtgcgttga ttctagaaac actggcacaa gcaggttcga tacttatcct
    10081 aaaatcagaa gagtttctag gaaaaagagc ttatatcggt gggatcaata aggccaaatt
    10141 ccgtcaaaaa gttgttccag gagatgtgct gaaatgtcat tttgaaatca ccaaaatgaa
    10201 gggatcagtc ggtacagcgt tatctgcagc atatgtagat gataaaaaag tctgtgagtg
    10261 cgagttcacg ttcatcgtca accaaaatgc ttgactataa aacctgatat agcaaaacaa
    10321 aaggaagctt acttgctttt tatgtaaaaa gaagacgcct gaatcctagc atacgttgtc
    10381 atctatgcag gattcagacg ttattttttt atttttgttt tgcttcttgc agtatattga
    10441 ttttatcgat ttttacttct tcttttggtt tgtctgaact atctgtttcg atatttgcaa
    10501 tagcatccac gacatccatt ccttcaatca cttggccgaa gacagtataa tggccatcta
    10561 gtgaaggata accgccgttt ttataagcgt cgatgattgc ttgaggatag tcgtctttta
    10621 aaagaccatc atgcatatca tctgtatttt gaacgataaa gaactggctg ccgttactgt
    10681 taggatcttg tgagcgagcc atagataacg cacctcttat attataaagg tggttgttga
    10741 tctctgtttc aaatccttct ccccaaatgc tttcccctcc agtgccatcg cctttaggat
    10801 cgccaccttg gatcatgaaa tctttgatga cgcgatggaa agtcagtccg ttgtagtaac
    10861 cgtcttttgc atgtgtcata aagttttcaa ccgcttttgg tgcgtgttcg ggaaataatt
    10921 tgattttgat tgtgccttta ttcgtcacca tttcgaccag atcttcattt tcggcgacat
    10981 ctttcgtcaa ttgtggtaat tccaatgcgt tcaaatcgac gctactggca gtcgtgcttt
    11041 ccgcagtaga tgtacttttt gaactttctg tagacgaact agaagaagtc gtgttgtttg
    11101 cagaacaacc agcagcaaac agcaatgctg ctgctaaact aatacttaaa ataactttat
    11161 ttttcttcat aactgttcct ttccaattta ctattttgtt atctttaatg ataacaaaat
    11221 agagggaaaa tacctactta aattattgga atttatcatt tattttaaaa gaaaaagaaa
    11281 aggtgtagac catttacttt gtgtttgcta taatgtaagt ggatacaaaa acgaaaagat
    11341 gcaggaggaa atggtaatat gggaaaatta ggaatctcga tttatccaga acggtcaaca
    11401 tttgaaaagg acaaagcgta cttggattta gctcataagt atgggtataa acgggttttt
    11461 accagtttgc tgcagatcaa tgatgataaa gaaaaagttc tttcagaatt taaagaagta
    11521 gttgattacg cgaatagcct aggcatggaa gtaatggttg atatcaatcc agcgttattt
    11581 gaacagttgg agatttctta cgatgactta tcttttttcc ataagatggg tgcctacggg
    11641 gtccgcttag atatcggatt cacaggagca gaagaagcaa aaatgacgcg caatcctttt
    11701 ggtatcaaaa tcgagatcaa tatgagttcg ggcacgaatt acgttgacaa catcatgagt
    11761 tattcgccaa acacggataa tttattaggt tctcataatt tttatcctca tcgatactct
    11821 ggtttaggat acgaacactt tgttttttgt tcagaaaaat tcagaaaata taatttgaat
    11881 acgatggcat tcgtcaattc gcaatctgct gagtttggcc cttggcctac gcaagatggc
    11941 ttgtgcacgc tggaagacca ccgagatctt gaaattgcga cacaagtgaa gcatttaatc
    12001 cttacaggat tgattgatga tatttctatt ggaaatgcgt acgcaagtga agaagaattg
    12061 aaagaaatgg cagaagcatt taacgcggat tatccaacat tgaaagtaga tacggaagaa
    12121 ggtattacag aaaacgaacg aatctgcttg tttgataacc ttcatagtta tcgaggggat
    12181 cgctcagaat atatccttcg ttcaacgatg actcgtgtat actacaaaga taaagatttc
    12241 ccgcctcata atacgcgaga tatgcatcat ggggatgtac tgattgacaa tgaagggtac
    12301 ggtcaataca aaggagaaac acaaatcgcc ttgaaggata tgaaaaacga cggacgtgtc
    12361 aatgttgtag gaagaatctc cgatgacgaa ttattcctcc ttgacttttt gaaaccatgg
    12421 tcaagcttta aattaattga aaataaaaaa tagataatta gttattgcag aaataatccg
    12481 ctagaaatag ccagttattt ctgctttttt attttgtttt tttatagg
//
LOCUS       DAYYAO010000079        12432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000079 DAYYAO010000000
VERSION     DAYYAO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.082"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACHARI_002164"
     CDS             complement(<1..190)
                     /locus_tag="ACHARI_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443185.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="ACHARI_002165"
     CDS             complement(200..1015)
                     /locus_tag="ACHARI_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8443186.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="ACHARI_002166"
     CDS             complement(1277..1720)
                     /locus_tag="ACHARI_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8443187.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="ACHARI_002167"
     CDS             complement(1854..2192)
                     /locus_tag="ACHARI_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8443188.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="ACHARI_002168"
     CDS             complement(2180..2557)
                     /locus_tag="ACHARI_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8443189.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="ACHARI_002169"
     CDS             2781..3974
                     /locus_tag="ACHARI_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8443190.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="ACHARI_002170"
     CDS             complement(4140..4562)
                     /locus_tag="ACHARI_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443191.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="ACHARI_002171"
     CDS             4957..5151
                     /locus_tag="ACHARI_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443192.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="ACHARI_002172"
     CDS             5148..5594
                     /locus_tag="ACHARI_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443193.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="ACHARI_002173"
     CDS             complement(5804..6976)
                     /locus_tag="ACHARI_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8443194.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="ACHARI_002174"
     CDS             complement(7182..7997)
                     /locus_tag="ACHARI_002174"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8443195.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="ACHARI_002175"
     CDS             complement(8147..8593)
                     /locus_tag="ACHARI_002175"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8443196.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9105)
                     /locus_tag="ACHARI_002176"
     CDS             complement(8590..9105)
                     /locus_tag="ACHARI_002176"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8443197.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9178..10092)
                     /locus_tag="ACHARI_002177"
                     /pseudo
     CDS             complement(9178..10092)
                     /locus_tag="ACHARI_002177"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10089..12416)
                     /locus_tag="ACHARI_002178"
     CDS             complement(10089..12416)
                     /locus_tag="ACHARI_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8443198.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2272 g         4217 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aa
//
LOCUS       DAYYAO010000080        12206 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000080 DAYYAO010000000
VERSION     DAYYAO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.083"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..777
                     /locus_tag="ACHARI_002179"
     CDS             205..777
                     /locus_tag="ACHARI_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8443199.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            922..1626
                     /locus_tag="ACHARI_002180"
     CDS             922..1626
                     /locus_tag="ACHARI_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8443200.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1854..2192
                     /locus_tag="ACHARI_002181"
     CDS             1854..2192
                     /locus_tag="ACHARI_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443201.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2177..3031
                     /gene="lgt"
                     /locus_tag="ACHARI_002182"
     CDS             2177..3031
                     /gene="lgt"
                     /locus_tag="ACHARI_002182"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8443202.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3178..3411
                     /locus_tag="ACHARI_002183"
     CDS             3178..3411
                     /locus_tag="ACHARI_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8443203.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3609..5513
                     /locus_tag="ACHARI_002184"
     CDS             3609..5513
                     /locus_tag="ACHARI_002184"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8443204.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5823..6185
                     /locus_tag="ACHARI_002185"
     CDS             5823..6185
                     /locus_tag="ACHARI_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFM8443205.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6202..6813
                     /locus_tag="ACHARI_002186"
     CDS             6202..6813
                     /locus_tag="ACHARI_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFM8443206.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6915..7823)
                     /locus_tag="ACHARI_002187"
     CDS             complement(6915..7823)
                     /locus_tag="ACHARI_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8443207.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7865..8179)
                     /locus_tag="ACHARI_002188"
     CDS             complement(7865..8179)
                     /locus_tag="ACHARI_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8443208.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8478..10409
                     /locus_tag="ACHARI_002189"
     CDS             8478..10409
                     /locus_tag="ACHARI_002189"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8443209.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10702..11253
                     /locus_tag="ACHARI_002190"
     CDS             10702..11253
                     /locus_tag="ACHARI_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443210.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11566..12108
                     /locus_tag="ACHARI_002191"
     CDS             11566..12108
                     /locus_tag="ACHARI_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFM8443211.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         3971 a         2041 c         2565 g         3629 t
ORIGIN      
        1 aaaaagatgt tggtgaagtt caccaacatc aaaaagtata gagcctgaat ttaatagaga
       61 agcctggcgt ctattaagaa ataattttct tgatgagcgt caattttttt aacggaaatc
      121 tatcctatac taaattcagt aaagaaaaag gaggaatcaa agaaagctat tttaaacaaa
      181 ccaaaattat aggaggaata aatcatgact gaaaataaag ctactcaaga aagaacaccc
      241 caagaaagat tgcaagaaga acaagaacac aaggaacatg ttcatcatac gaaaattaat
      301 gcagcggcca ttgcggatca tcttttaggc aacatgcata cattacatgt gaaactgcac
      361 caatatcact ggtatgtaaa aggagccaat ttctttacgt tgcatgaaaa atttgaagag
      421 ttgtataacg aaaatgaaaa atggttcgac aaacttgcgg agcgtctaat tacttctggg
      481 cataaacctg cttcgacaac ggttgaattt gaaaaatatt cgatgctttc ggaagatgca
      541 gttaataaat acgctaaagc agaagacatg gttgaaaata ttatcgagga cttcagaagc
      601 actcgtgacc taactattcg cgcgattcgt ttagctcaag acgagggtga cgatgcttta
      661 gaagatacat tgattgcttt taaaaatgac ttagacaaga acatttggat gctgcaagcg
      721 ttcattggta aagaagcatt agaagatgat gacgctcttg atgaggatga agattaaggt
      781 aaaagtagaa gctgatttag ggattggaat tctttccagt cctttttcca ttttcttgat
      841 gtgcatcaat ttttttgtag taagagactt atataataaa agcagcgaat ataaacaaaa
      901 taaataaagg gggagaatga attgactaaa catacccatc atgaaattgg cgaccacgca
      961 gcctgtatcc gcttggttcc aatattcaat cacttagagg atagcgcgat gaattacatt
     1021 gctgaaaaag cccacacgaa acagtatcaa aaaggagagt ttctgtttcg ttcacaagaa
     1081 aaagaggata ctttatatat tatcaatcgt gggaaaatcc gtatttatcg attggcagac
     1141 tctggcaaag agcagctagt gcggatcttg aaccctggtg atttcaccgg agaatggacc
     1201 ttgttcaacc cggatgcagt acacgaggaa tatgccgaag caacccgaga taccgttgtc
     1261 tgtatgattc agcaaaaaga tgtccaagat tttctggaac aatacccagc tatttcttta
     1321 aaactattgg cggaaatgtc taaacggtta gaaagttcag aacgtcaaac gacacaagta
     1381 gccgtggaga gtgtcattac ccgtttagtt ttgtttttgg cagaaaatgt ggaacctgaa
     1441 atgggcaact ctcccaccat caccctgccg atggcaaaaa aggacattgc ttcctattta
     1501 ggaacgacac ctgagaccat cagtcgcaaa tttggagaat tggaggacga aggattgatt
     1561 caacagctgt ctgggaaaag gatcaagatt caggatttag atgatttatt gctttacagc
     1621 gaataatcgg acacactttt ttctacacgt tggtgttctg tcgctcttca ttaacaacgg
     1681 gacagaactc agtgcttttg cttccatctg tccgtttttg gcttttttaa gacagaacga
     1741 ccttcattca ttggaaaaat aaaagcacaa acatgataac cctcaagttt ttttgtttta
     1801 tttttcttta agatagggtt gtcgaacaaa acgtaaaaat aagtagggtg aatgtgcatt
     1861 gtttttgggt gatagttgcc atcacgttgt cagcagcggg ggcattcttt gtcgtccctt
     1921 tcaccaagaa cacgattggc ggcgaagcaa aaacgtctaa cgttgttgag gcagataggt
     1981 tagctatgga aaaagaagag gaagttgtaa aaggagccgg cgtaatcgga cacggaaaag
     2041 tggcgtatta cgaagcgagt gaccagggag ataaaagtgt attcactgtt tggtttccat
     2101 ggagcttagc cggaatcttg ttcagcatca ccatgactag aatgacgcga aaaattgaag
     2161 aaatggagca aatagaatgg gattcgtatt aggcgttttg aatccccatg tggtggagat
     2221 tggttctgtt gtgattcatt ggtatggagt cattattgca gcgggcatat tagcggctct
     2281 ccagttgagc accaaggaag caaagaaaaa aggactggaa gaggatacca ttatcgatat
     2341 ggccttatgg gcaattccga ttggactcct cggcgctcgg ctttattatg tgttatttga
     2401 acttggttat tatctgcaaa atccaggaca aatccttgcg atttggaatg gcggcattgc
     2461 gatttatggg ggattgattg caggaggggg tacgctatat tggtatgcaa agaaaaaagg
     2521 tacttctttg gcactcgttc ttgatatttt agcgcccaat gtattgttag cccagtccat
     2581 tggtcgttgg ggcaatttta tgaatcagga agctcatggt gggccggtta ctcgtcaatt
     2641 cttggaaaat ctttatttac ctgaatttat cattgaacaa atgaatatga atggtaccta
     2701 ctaccatccg acttttttat acgagtcatt gtggagtttg ttgggctttg tcctcatcgt
     2761 cactatacgg aaccgaaaac agcttttgcg tcaaggagaa gtcgctttga gttacgtttt
     2821 gtggtactca gtgggtcggt tcttcattga aggtatgcga acggacagtt tatggattgg
     2881 cgagtggatc cgcgtttcgc aagccttgtc tctggccctg tttattggcg cgattgtagt
     2941 atggtttata cgcagacaag attatccacc aatttcttat tattctgatg gcaataaggg
     3001 gcagaaaaag actattaaga tggtgaattg agcaaaaaga taaaaagaag tgcaaaactt
     3061 gatatgcgtc aattttcgtt tgctaaaagt gagctatact acagttgtaa agaggaaaag
     3121 gcagtgttca ttaatctgct gccgataaaa taaaaacaca taaaggagat aggaattatg
     3181 gaaaaagcta tattgcaatt agaaacgttg tcttgtccaa gttgtatgca aaaaattgaa
     3241 ggtgccgtga aatcagttaa cggtattgat aaagacagca ttaaagtatt attcaactcc
     3301 agcaaagtca aaaccaattt tgattcagct gtcacttcga ttgaagagat tcaaaaagct
     3361 atcgaaaacg taggctatcc ggttcttaaa gcaaaagtca aggccgttta acagattaaa
     3421 tgaactaggc aattctggaa gaaaagctat ttttccagga ttgctttttt tgttgcaatc
     3481 cgattcgagg tgcttagaaa cgataaaaga gaactttcta aacttgatgt cagtcaatta
     3541 attacttagg caattatctt atactgtgtt tatcaataaa agagaaatta aatttggagg
     3601 gaaaaagcat gcaacaatat atattaagca agaaaaatgt tatcaccgtc atcagtggat
     3661 tgttaatcgt actcggtttc ttcagtcact ttgttttaga aaacgtagga ctttcagagt
     3721 ggtctttgat catcgcctct gtctttggga ttacgccaat tgccattcaa gcgtttcaag
     3781 caatgaaagt caaagtcatc agtattgatg tcttagtcag tattgcagcg attggtgcgc
     3841 tttttattca aaattatgaa gaatcggcta ttgtcacgtt cttattctta ttcggacact
     3901 acttggaaca acgaacattg aaccaaacgc gatctgctat caaagaattg actgaaatgg
     3961 cacccgaaag cgccttgaaa caaatggata atggcgaatt tgaagaagta gaagtggatg
     4021 atatagatga aggggatatc ttgctcgtta aaacgggtgc gaaagttcca gtagatggca
     4081 tagtccttac gggtgaaggg catatcaatg aagctagcat tacaggtgaa gctattccgg
     4141 tcaacaagct agctgatgca gaagtttttg caggaaccat tttagaaaac ggaacgattc
     4201 aaatcagagc tgaccgagtt ggtgaggaca ccacatttgg aaaaattatt gaactcgtgg
     4261 aagaagcaca agattctaaa tccgaagcgg aacggttcat tgatcgcttt tctaaatact
     4321 acacaccagc tgtcttggtt ctggcaattg ttgtatgggc gttcagtcaa aatattgagt
     4381 tagcaatcac catcttggtt ctaggttgcc caggcgcatt agttatcgga gtgcctgtct
     4441 caaacgttgc cggtattggg aatggtgctc gtaacggtgt tcttttaaaa gggagtgaag
     4501 tcattcaaga cttcagtaat gtggatacaa ttgtatttga taagacaggt actttaactg
     4561 tcggaaaccc aaccgttgca gcgactgaac tgtatgaaaa agatcctgct gaaacgcttg
     4621 gctatctggc cagtgtggaa cgggaatcag atcatccatt agcaaaagcg gtcttaaatc
     4681 aaattggaga aacaaacttt tatcctgttg aaggaactga agttgtgaaa ggcggcggaa
     4741 tcgtttcaag tgtagctgga catagagtgg ctgttgggaa tgtggccttg atggaaaaag
     4801 aaaatgtcac tctcagcaaa aaagtgcaaa aagatgtcaa acggtttgaa caacaaggga
     4861 actctctcgt tttgacagcg gtcgacggtg aattaaaagt actgatgggc attcgcgatc
     4921 aagtccgtcc gggtgtgaaa gctaatttgc aggaattaaa agacttgggc gtgaaaaatc
     4981 tagtcgttct ttcaggagat aaccaaggaa ccgttgatgt ggtcgccggc gaacttggtt
     5041 tgaccgaagc tcacggctac atgttgccgg aagacaaatc ggcttatatc ggaaaacttc
     5101 aacaacgtgg tcaaattgta gcctttgttg gagatggcgt taacgatagt ccgtccttag
     5161 ctttagcaga cattggaatc gcaatgggaa gcggaacgga cgtagcgatt gaaacatccg
     5221 atgtcgtttt aatgaactcg aacttcagca acttgcctca cgcattagga ttagtaaaag
     5281 ccaccgcaaa taatatgaaa caaaatatcg tgatttcaat tggagtagtg ttggtcttgt
     5341 tgaccagcgt cttcttcagc gaatggatga atatgtctat cggaatgttg gttcatgaag
     5401 gtagtatctt ggtggtaatt ttcaatggca tgagattaat gaagtataag ttgaaaggtc
     5461 gaaaagaaca aaaagaagta tcagcacctg cagagaaagt aaaaatatct taagaataaa
     5521 ttagaagagg ctgagacaat agtccatcct tgaaatgaca aaagcggaaa ctcagatttt
     5581 taaatctggg attttccgct tttttgagtt cttgaaaaaa aataagagta tagagtatgt
     5641 tctttataaa attaattttc gtgtaaatga ttaaaaaatt taataataaa atttaaattc
     5701 ctattaagtt gtaacacatg aaattgctta taatagtgta ttgaaattaa ggtagattga
     5761 caaacgagtg atcaaataga tatactacct tcaaacgacc gtttgaatgt gaggtgagat
     5821 gaatggaaaa agtttgccaa gtgactatcg tacataaaga aaaagtaaat gtaataaaaa
     5881 aaaaattgga tcaaaaagat ttttcaaact tacttgtgtt agggaaatgt ttcagcgatt
     5941 cttctaggat taaaattttc tatgctttag aaacctataa ggaaatgtgt gtctgtgatt
     6001 tagcagaaat acttactgct agtgtcgcta caacgtcaca ccatttacgt tttttaaaaa
     6061 aacatggaat ggcaaaatca cgtcaagatg gaaaagtagt ttattattct ttagcgaatg
     6121 aagacatcct ttccgctatt cgtgtttttt taaagttatc agaaaatcta tctatgaaat
     6181 cttagtttgg aggaagttat tttgttacaa agtatttttt cagctatcgc tgtttatatt
     6241 tctaccagta ttgattattt gtttatcttg ctgattatct tttctcaaag ccacacaaaa
     6301 aaaggtattc gtcacatttt ttggggtcaa tatcttggaa caggtatttt ggtagccgta
     6361 agtttgtttg ctgcttatgt gctaaatttt attccgcaag attggatcat tggtcttctc
     6421 ggtctaattc cgattttctt aggaatcaga gtcgcttttg ttggcgaaga ggaggaagaa
     6481 gaagaggaag tcgttgaaaa actcgaatct agaggaacta atcgcttatt ctggacagtt
     6541 gccttaataa cgattgcttc tggaggcgat aatttaggta tttatattcc atactttacc
     6601 tcgttagctt tttcagaaat cattattgct ttaatcgtat tcgctatctc tgttgcggtt
     6661 ctctgctata tcagttacaa attagccaag atttcttttg tttccgaaac gttagaaaaa
     6721 tacgaacgaa tcattgtccc tataatgttt attgcgttag gaatttttat tatgatagag
     6781 aacgggacga tacaaacgct actaggtttt taaaaatcat aatcacgaaa gataaaaaga
     6841 gaggcagcga ctaatcaaaa tcgctgcctt ttttgctttt caaataatga agtatttttt
     6901 agcgtagaac tatcttacgt gttgtgggag tatccctttt caagctcttc tttgcttgta
     6961 gttatttcta ttgtgatatg attctctacg cttgatttgt gtgcgacttc tgcaatttct
     7021 tcttttattt ccctagcctt gtctatgtct gaagggtcta taagaaccgt gatgataagg
     7081 gcgttttctt ccccgtccag tgaccaaatg tgaaaatgtg agaggtcttt tacagcagga
     7141 acatttttaa tggagtccgc caagctcttt acgtttacat tttcaggcga accattcaat
     7201 aaaattttca ttgtggtgaa aaattcagga acggttttgt agaggatata aagtgcaatc
     7261 aaaatcgata agataggatc cagacgatat gcctcggtaa agttcattag aatactcaca
     7321 atcaagactc caatccaccc taatacgtct tccagcatat ggaggttcaa catgctctcg
     7381 tttttggatg tgcctttgct taaaatccac gcagcatatc cattcaatgc aatagctacg
     7441 agagaaagcc agaacatgcc actataattt acgggttgtg ggtcaatcaa acgtggcaca
     7501 ctacgataga tcataaaaaa cgagccacta attagcacaa cgccagttat cagcgcgccc
     7561 agtaaagaaa agcggttata gccaaaagtg aaccgttcat cttgtttttt ctctgaataa
     7621 ccttgaaaaa accaagcgaa tccaattgaa atagagtccc ctaaatcatg aactgcgtcg
     7681 gacataatgg acacactatt gaaaagaaag ccaaagaaaa attcgcttat agagaaaata
     7741 agatttatga agaaagcaat tttaatattc tttttcggtt gattctgatt cgagtgatta
     7801 tggtctttat ctgattttga catttaaaaa cctcctaagt ttgtatgcta catactaatt
     7861 ggttttattt ttctaattca ttaacatgag ttaagacttg ttcgagaata ctaaaaacat
     7921 gaaggtcatc taaactgtaa agcatacttt ttccttctct tcttgatttc actaagcgtg
     7981 cgtctttcaa cgtttttaat tgatgagaaa tagcggactg ttccatgtcc agagaaatag
     8041 cgatatttcc aacactacac tcttttttct gtaaaagaaa gagaaccgac aatcgtgtag
     8101 gatcactaat caccttaaat agtttgctta ttgacagaat agatccttta tcgattggag
     8161 aagttgtttt tatatccata gtgtttttat tcctttcata tgaccttccg ttcatatcta
     8221 tattaccata tgagcgaaac aatgctaatc ttttgtttat aaaagagaag tggcgttctc
     8281 tttatcttga caaaatgtat atagcgatat aaaatgagta tacaaacata tgattaatta
     8341 gtcatatgta aggtggattc attacgattt acttttggaa taaacgaaag gagtcagaaa
     8401 tagcagaaat ttatgtgatt caagaagtaa atgatggaaa acaaaggaat tataaattat
     8461 atagggaggc aatagaagtg gaaactatcc aagaacaaca gtctcaagaa aagcataatc
     8521 ataatcacaa ccacgatcac gaccatgatc atgggaaaat gccagtcgtt ttgtactttg
     8581 ttggtttagc attagcagtg attgctctat ttttgaatga agattatcag ttggtacaaa
     8641 atgttttgtt ctcaatcgct acaatcagtg ccgggtatca cgttattctt cttgaaggcc
     8701 ttggagaaac aattgagaat tcaaaagcac aaaaaaagtt catacctaat tctcatattt
     8761 taatgggatt agcggcaatt ggagcttctt tgatggggaa tttttgggaa ggaacattat
     8821 tgattcttat tttttctggc gcacattttt tggaagatta tgcagaagga agaagcaaac
     8881 gagaaataac gaaattactc gaaatgaatc caacgactgc ccggttaatc ctgcctgatg
     8941 gtagtacaaa aaatgtggat gtcagtgaat taaaagtggg agatcaactt caagtgttga
     9001 atggcgatca agtacctatt gatggcgtca ttttgtctgg atctacatcc attgatgagt
     9061 cgtctattac tggagaaagt atcccgaaag agaaatccaa aggagatggc gtcttcggga
     9121 gtacaatcaa tggaacaggt accttcacga tgaaagtcac caaagaaaac aaagatacag
     9181 tattttcaaa aattttacaa ttagtgaatc agaaccaaga taaccaaaca aaagctgcga
     9241 gtattatcca aaaatttgaa ccgaaatatg tcacagttgt tttaattgcc attcctctat
     9301 tcatgttatt ggctcctttt ctacttgatt ggacatggtc acaaagtgtc tatagaggat
     9361 tggtcctttt agttgcagct tcaccgtgtg ctttagcagc agctactgtc tcggcaacgc
     9421 tatcgacgac ttctaattta gccaaaaaag gagtcctttc aaaaggtagt tcttacctgt
     9481 ctcaattagc cgatattaaa gcaattgcat ttgataagac gggcaccttg accaaaggaa
     9541 aacctgaagt aaccaattat tattttgctg attccgtgaa cgaagaaaac atgattgata
     9601 tcgtagtagc tcttgaaaaa gaatccaatc accccttagc agatgctatt ctaagaaagt
     9661 ttgagcaaaa aaacaaacta actattgaag tagaaaacca aattggtaaa ggattgacgg
     9721 gagattacaa agggagaaat tatcgtatag gaaaaccgac ttcgtttgat gatgtttcag
     9781 atgaatatag tcgtttaaat aatgaatggg cgtcagaagg taagacagtc gtatacgtag
     9841 cagtagatga agaggttata ggccttattg cccttatgga cgttccgagt gaatatgcta
     9901 aagaaaccat tgaatatttt aggaaacaag gtatacacac cacattaatt actggtgact
     9961 cagaaatgac gggaaaagcg gttgctaaac aattaggaat agatgaagtg attgcgaatg
    10021 tcatgccgga agacaaatcc agaattatag acgaacaaaa agaaaaatac ggcgtgaccg
    10081 ccatggttgg agacggtgta aatgatgctc ctgctcttgt taatgcagat gtgggaatcg
    10141 ctatgggaga tgggactgat gtggcagtag aggtatctga tttagtttta atgcaaaaca
    10201 atttatctaa attggtacaa tctcataata tttcttcgaa aatgaaccgt gttatttggc
    10261 agaatattat tttttcaatg gccgttgttg cttttttaat tgtcgttagt ttcttaggct
    10321 taacggatat tgcaatcagt gtcattattc atgaaggaag tactttggtt gttattttga
    10381 atggacttcg attgctaaag gcagtctaaa gaatgattgc acaacttcaa attaatcgag
    10441 taatagagat aattaaggat tacactacat tttttatctc tattacttgt ttatatttat
    10501 ctagatgttc aaagtcaaaa tgatatatca accatactct tgtggcgcgg gttgaatata
    10561 agatacatga aattggataa aatcatgtat caagaatcct atccttatag ggttcttttt
    10621 ttttactccc cctaaacaca cgatttcgtg ttgtaataac tataatcatg tattcgaaca
    10681 gactaaaaag gagggattgt catgggctat aatgttcagc cactacggtc gcaacaggag
    10741 attaatgatt ttttattttg cctgcgacgg aataaaaatg cggaacgtga cgttttcttg
    10801 tttctaattg gaattaacag cggtctgcgc atgtccgaca tcgttaaact caagaaaaaa
    10861 gacgtgatta cctcaaaaaa cccgcgcatc gtggagcaaa agacagggaa aacacgcatt
    10921 ttatacttaa gcagcttaca ggacttgatt cacggctata ccgcaccctt agatccggag
    10981 gcttacttgt ttcccagtac taagggcggc catttagaag tcaatacggt ctaccagatg
    11041 tttcaaaagg tcgcgaagct gttaggaagg gacgatatcg gcacccacac cctacgaaaa
    11101 acctttggtt accactatta caagaaaacc aaagacgtgg ccaccttaat ggaaatcttt
    11161 ggtcacagca gcgagaagat taccaaacgc tacatcggaa ttaatgaaga tgaaatcagt
    11221 gaaacgttat taaattttcg gttaggtttt tgattgtatt atataataga taagcacagt
    11281 aagacggtta taatcaccca cagtattgga caaatcaacc tttttttggt atattatata
    11341 tacaaatgag agcaggctca tagtcaactt gatataaatc cggaacataa gggtccggcg
    11401 ggtggcgagc accaaaacta aactcgatac aaatccggaa cgtaagggtc cggcgggtgg
    11461 caagcaccaa aactaaacct gtacaaaagg ctgctctttt gagcagcttt ttttattggg
    11521 aaaaaggagc attcttcgca cgcggcaaaa aaagaataca aacgcatgtc tataaaagag
    11581 acaacccaaa ttaccagaca gttaaacgcg atttataagg ctgctcatct cttacaagaa
    11641 cattttgtag ataaaaaagt atcctttgtc ggagaagtct caacggtagc gattattttt
    11701 tcgacaacta attttatgca cctctgtggc attgattatc gcagaggcac acacttgttt
    11761 tttcaagatg ccttagacag gaaaataaat ttacaggata ttcaaatcaa aacggatggg
    11821 acgacctttc agaaattaca agtaataggt agccttgatt tattattggg gaagcatatt
    11881 tctattgttg gcagaggtgt ttactcttct cttcgctatg atgcagctat tcgaacaagg
    11941 aaaaaaatat tggctctgtc tttgaaacaa aacggattaa tctatatccc tatctctttg
    12001 ttaaacctat catcaaagga aattggacct ggacaaaaag ttactggcat ttttagcgag
    12061 gatttaacat ccggggaact taaaatgatt atggaagtca tcgattaatc attaatagtg
    12121 atatgcgacg tcattttgaa gtaattattc tattttgggt tataaaataa agcattttaa
    12181 gacgatttca aatcattcat gtgggt
//
LOCUS       DAYYAO010000081        12144 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000081 DAYYAO010000000
VERSION     DAYYAO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.084"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACHARI_002192"
     CDS             <1..566
                     /locus_tag="ACHARI_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8443212.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /gene="fic"
                     /locus_tag="ACHARI_002193"
     CDS             967..1572
                     /gene="fic"
                     /locus_tag="ACHARI_002193"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8443213.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="ACHARI_002194"
     CDS             1588..2160
                     /locus_tag="ACHARI_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8443214.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="ACHARI_002195"
     CDS             complement(2412..2669)
                     /locus_tag="ACHARI_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443215.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="ACHARI_002196"
     CDS             complement(2795..3610)
                     /locus_tag="ACHARI_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443216.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="ACHARI_002197"
     CDS             4127..5452
                     /locus_tag="ACHARI_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8443217.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="ACHARI_002198"
     CDS             5445..5795
                     /locus_tag="ACHARI_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443218.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5792..5962
                     /locus_tag="ACHARI_002199"
     CDS             5792..5962
                     /locus_tag="ACHARI_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443219.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACHARI_002200"
     CDS             complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACHARI_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8443220.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="ACHARI_002201"
     CDS             complement(7113..8066)
                     /locus_tag="ACHARI_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8443221.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="ACHARI_002202"
     CDS             8678..10171
                     /locus_tag="ACHARI_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8443222.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="ACHARI_002203"
     CDS             10306..10602
                     /locus_tag="ACHARI_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8443223.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="ACHARI_002204"
     CDS             10703..11554
                     /locus_tag="ACHARI_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443224.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="ACHARI_002205"
     CDS             11575..11787
                     /locus_tag="ACHARI_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443225.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4171 a         1864 c         2268 g         3841 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca cctggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaa
//
LOCUS       DAYYAO010000082        12113 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000082 DAYYAO010000000
VERSION     DAYYAO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.085"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..552)
                     /gene="frr"
                     /locus_tag="ACHARI_002206"
     CDS             complement(<1..552)
                     /gene="frr"
                     /locus_tag="ACHARI_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8443226.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLE"
     gene            complement(555..1277)
                     /gene="pyrH"
                     /locus_tag="ACHARI_002207"
     CDS             complement(555..1277)
                     /gene="pyrH"
                     /locus_tag="ACHARI_002207"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8443227.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1413..2294)
                     /gene="tsf"
                     /locus_tag="ACHARI_002208"
     CDS             complement(1413..2294)
                     /gene="tsf"
                     /locus_tag="ACHARI_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8443228.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2393..3175)
                     /gene="rpsB"
                     /locus_tag="ACHARI_002209"
     CDS             complement(2393..3175)
                     /gene="rpsB"
                     /locus_tag="ACHARI_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8443229.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3534..4013
                     /locus_tag="ACHARI_002210"
     CDS             3534..4013
                     /locus_tag="ACHARI_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8443230.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4230..5321
                     /gene="phnW"
                     /locus_tag="ACHARI_002211"
     CDS             4230..5321
                     /gene="phnW"
                     /locus_tag="ACHARI_002211"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8443231.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5314..5442
                     /locus_tag="ACHARI_002212"
     CDS             5314..5442
                     /locus_tag="ACHARI_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8443232.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5446..5991
                     /locus_tag="ACHARI_002213"
     CDS             5446..5991
                     /locus_tag="ACHARI_002213"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8443233.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6445..8136)
                     /gene="argS"
                     /locus_tag="ACHARI_002214"
     CDS             complement(6445..8136)
                     /gene="argS"
                     /locus_tag="ACHARI_002214"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8443234.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8556..9503)
                     /gene="arcC"
                     /locus_tag="ACHARI_002215"
     CDS             complement(8556..9503)
                     /gene="arcC"
                     /locus_tag="ACHARI_002215"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8443235.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9618..10637)
                     /gene="argF"
                     /locus_tag="ACHARI_002216"
     CDS             complement(9618..10637)
                     /gene="argF"
                     /locus_tag="ACHARI_002216"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8443236.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10728..11957)
                     /gene="arcA"
                     /locus_tag="ACHARI_002217"
     CDS             complement(10728..11957)
                     /gene="arcA"
                     /locus_tag="ACHARI_002217"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8443237.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3307 a         2518 c         2192 g         4096 t
ORIGIN      
        1 ttccaataat tctttttctt tatcagcagc gatcttatct acttctttga tgctgttatc
       61 tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca tcttcggtaa tatcaccatt
      121 tttttgttgt tttttatatt catcgatcgc atcacgacgg atgttacgaa tcgcgatttt
      181 tgcattttct gcttcttttt taacatcttt agctaattct tttctgcgtt cttctgtcag
      241 ttgcgggata actaagcgaa tgacatttcc atcattggtt ggactgattc cgatatcact
      301 tgccataata gctttttcaa catcttgaag aatgctcttg tcaaaaggtg tgatcattaa
      361 aacacgtgct tctggaattt ggattgatgc catttggttc aatggtgttg gcgcaccgta
      421 gtagttcaca gtaatacggt ccaaaaggct agcgttcgca cggcctgcgc ggatttgccc
      481 aagttcacgt tgaagatttt gtgctgcttt tttcattttt tcttttgctt ctgtcataat
      541 tgcatctgcc atttttattt cccccttaca gttgttccga tattttcgcc taagattgca
      601 cggcgaatat ttccgtgttc atttaaattg aagacaagca gtggaatatc attatccatg
      661 cttagtgaac ttgcagttga atccatgact tgtaatcctt tggcaataac ttccagatga
      721 gtcagttctt caaattttac tgcatttgca tctactttcg gatcagcaga ataaacaccg
      781 tctacattgt ttttagccat caggataaca tctgcgccga tttctgccgc gcgtaatgct
      841 gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc ctgcaaagat aacgatccgt
      901 cctttttcta aatgacgttc tgctttacgg cgaatatatg gctctgcgat ttgacgcatt
      961 tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt ccaacgtatc ctgtaaagct
     1021 aaggcattca taactgtagc taacataccc atgtaatcag cttgagcacg ttccatgccc
     1081 atttgtgctc cgatttgtcc gcgccagata tttccgccgc ccacaacgat tgccatttca
     1141 acgcccagct cgtgaacttc tttgatttct tggatgattt ctttgattac tggtggtttg
     1201 attccaaatc ctgcgtcccc agctaaagct tctccactca attttaagac tacacgctga
     1261 tatttcggtg tgaccatgtt cattcctcca atattttcta ttgccatttt accatactta
     1321 gatgatttcg acactctaag actggttttt cttgttttgc ttaaaaaaag ggagcgcaca
     1381 aaccgtgcgt tcccgtctgt tattcaacag aattattttt tcacttggct cataacttct
     1441 tctacaaagt tatcttcgcg tttttcgata ccttcgccaa cttcgaaacg tacgaatgtt
     1501 ttaacagttg cgccttttga tgcaacgtat ttttcaacag tcatatcagg atctttaacg
     1561 aatggttggt ccaccaaagc gatttctgct ttgaatttct tcaagcgtcc ttctaccatt
     1621 ttttcaacga tattcgctgg tttgccttcg ttcaatgctt gttctgtaag aacagttttt
     1681 tcatgttcta attcagcttc tggaatttgt gattcgttta cataacgagg attgatagct
     1741 gctacgtgca tagctacgtc tcttgcaact gtttcgtcag ttgttccatc tagaacagtc
     1801 aatacggcaa tacgtccgcc catatgcaag tatccaccaa atgcagcatt gtcgtctttt
     1861 tctacaactt caaagcgacg gaagctgatt ttttctccga taacttgagt tgcttcgatc
     1921 aagtcagatt caattgtacc tttatctgtt ttgattttca ttgcagcttc catgtctgct
     1981 ggtttgtttt cagcaacaag ttctgcaatt tcttttacta aatcttggaa catttcgttt
     2041 ttagaaacaa agtctgtttc tgagtttact tcaacgattg ctgcagtgtt acctttcaca
     2101 gcaactgaag ccaatccttc agctgcaatg cggtcatttt tcttcgcagc ctttgccatt
     2161 cctttttcac gtaaaagatc aactgctttt tccatgtcgc cttctacttc aactaatgct
     2221 tttttcgcat ccatcattcc tacgccagtc atttcgcgta gttcttttac cattttagct
     2281 gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct ttttaaaaaa agctgcttca
     2341 aaggagagtg aggcttgtcg cccagccttt gaaacagctc catcattcaa aattattctg
     2401 ctgaagaatt gtcgccttca acaacatcta cgatttcttc gattgatgtt gctgtgtttt
     2461 cttctgtgaa atcttcttca actacttgat cttcaccttg gtttccttcg atgaaagcat
     2521 cagccatttt tgaagtaatc aatttaacgg cacgaattgc atcgtcgttt gaagggatta
     2581 ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat agctacgatt gggatattta
     2641 atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg gtcaacgatg tacattacat
     2701 ctgggattct tggcatatca gcgataccgc ctaagaattt ttcaagacgt tcacgttctt
     2761 tgttcaaacc agcaacttct tttttaggta gaacttcaaa agttccatct tcttccattg
     2821 cattgatttg tttcaaacgg ctgatacgtt tttggatagt atcccagttt gtcaatgttc
     2881 cgcctaacca acgatggtta acgaagtatt gtcctgcacg agttgcttct tctttgattg
     2941 cttcttgtgc ttgttttttt gtacctacga ataatgctac gccgccttct tcagcaacgc
     3001 tcttcatgta atcataagct gcatctacta atttaactgt tttttgcaag tcaatgatgt
     3061 agattccgtt tctttctgtg aagatatatt tcttcatttt tggattccag cgacgagttt
     3121 ggtgaccaaa gtgtacgccg gcttctagta attgtttcat agaaattact gccatgtttg
     3181 tttcctccga tttggtttta tttttcctct tcttatcttc taccttgccg gcaaactttt
     3241 tcaagcacct accgacaatc caataagaat gtggatttgc tgatgcataa agcaccctca
     3301 gctattatac agacaaaaga tccgtatcgc aagagcaaat atacttttct gctcaacatt
     3361 caatagttta cctgaaagat aaaaagaatt caagaaaaac agtagatttc tctttgtttc
     3421 tttattatcc tttttccata aaataatttt aggtataaaa gacgaatatt attgggcaat
     3481 ccttcttttt tttgttatga tacatgtgaa atctttcgta aaggagaaag aaaatgcgca
     3541 aagctcaaag acatttgctg attaaacaag tcattgaaga attcccgatc cgtacgcaag
     3601 aagaactatt aaacaaactt gggcattatg gcgttaccgc cacgcaggca acgatttctc
     3661 gagatatccg tgatctaaaa atcgtgaagg cacctgatga aaatggcgtt tcccgcttcg
     3721 tattgtttcg cgaaaaagga actcctgaga ctaggactga ggaagaggat cggctggttc
     3781 aaatgattga agacattgta ttgaaagtcg aacgagtcca ctttttgacg atcgtcaata
     3841 ccttgccaga caatgctcat ttatttgcat ctgttttaga tgagatcaaa cctccctcta
     3901 ttgtcagtac aattgctgga ttcgatacga tcatggtcat ctcggaaaat gaggaggacg
     3961 caaaacgagt agaagaattt cttcaccatc caactgaagt atcacttttt taatcagagc
     4021 acgtattcct aatatggaat gcgtgttttt ttattttcaa tattttcctt attgaataaa
     4081 cctttacact ctttacaaaa atacatcatt ttttattcaa atttttcaac accttgattg
     4141 aatcacaata atcatcattt atattggatg ttaacaagag cagcttcttg ctctttgtgt
     4201 atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt attaacttct ggaccattga
     4261 cgacttctga aacagtgaaa aatgctatgc tgatcgatca ttgtacttgg gatcaagaat
     4321 acaaagaaat tacacaatgg ataagaaaaa aattattgga tcttgctcaa gtatcggaag
     4381 gaatatacac aacggtctta atgcaaggta gcagctcctt cggggtagaa tcggtattaa
     4441 gttctgtgat caggccagaa gatactgtac tgatttgttc aaacggcgca tatagacaac
     4501 gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac cgtttatgag gtatcggaac
     4561 atcaaatcct ccaagctcat gtgattacac agctgattga agagaatccg tctatctccg
     4621 cactagcaat ggtccatagc gagactactt ccggtatttt gaataatttg gaagagattg
     4681 ctgctgtcac acaagaaaga gatcttttgt ttatagtgga cgcgatgtca agctttggtg
     4741 ggataccgat ttgtgtagcc gaactaggga ttgatttttt gatttccagc tcaaacaaat
     4801 gtattcaagg tgttccaggc ttttcttttg tacttgctaa taaagcggaa ctggaaaaac
     4861 gaaaaaatgt tagccgaagt ttgtcacttg atctatatga ccagtaccgc gtgatggaaa
     4921 acgagaatgg caaatggcgt ttcacttccc ctacccatac ggttttggct tttcaacaag
     4981 cgctgaagga actagaggca gaaggaggaa tcgaaactcg atattcacgt tatctcgcca
     5041 ataatcatgt gctacgacaa aaaatgacag agatggggtt ccagcctttc attcaagaat
     5101 atcaggggcc attcatcacc tcttttcttt accctaagga ctggcgattt gattttaatc
     5161 atttttatca gtatttaaag caaaatggat ttgtgatcta tcccgggaaa atcagcgatc
     5221 atgaatgttt tcgtatcgga aatattggcg agatctatcc ggaagatatt ttggaactga
     5281 cagcaattat caaagactat aaggagaacg ttaatgatta aaactgtaat ttttgattgg
     5341 gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc acgcttttag aaatgctttt
     5401 ctagaaaaag gaatccaact gactgacaaa gaaatccgct aaccgatggg aaaattaaaa
     5461 tgggatcata tccaacagct tctttccctc ccttctgtca aaaaacaatg gatagctatt
     5521 tatggacaac tacctcagga aaaagaggtg gaggaattgt atcaacaatt tgaacattat
     5581 ctgtttaaag atttggtcaa gcattctacc ttgaaacccg acacattgga aacgatcgaa
     5641 cggttgagaa acgctgggat caatattggt tccacgaatg gatttaatgc tcctatgatg
     5701 acaatcgtag cagaaaccgc aaaaaaagaa aggtattcac cagacttcat ggcaactttt
     5761 gaagacgtcg aagggtatgg tcgaccgttt ccttatatga tccataaaaa catgcagtac
     5821 ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag atacggtctc agatatccag
     5881 gaagggaaaa atgccggagt gttgacagta ggtgtaattg aaggtagttc gctaatgggg
     5941 atttcttatg atgagtacca aaaattagag atggggaaaa agatcgttta aaacagtctg
     6001 ttgctcttag attccaacaa gccggcgctg atatttgtgt aaacaattta aaagagttaa
     6061 cagaatacat tttgtacgct gacatgaatc cagaatgact tttatacaaa ataagaggtt
     6121 gtgaaaaagc tgtcctgtat caagtaataa aaacagccaa gcaaaaatag ctcctcatat
     6181 ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
     6241 aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc ttatttgcaa acaaacagtg
     6301 tttaagagac aacctctcca ctaaacggtg ttcattcagc tgactctcgg tatttgctca
     6361 aaaaacgata aaatttgaaa agctattttc tcttttgttt ctctaatact cggatcagaa
     6421 cgctgatttc acattctctt tttcttacat cttatctggt gcatgaagtc ctagcaaacg
     6481 aaggtcttct ttcaataata cagtcactgc ataaactaga gctaagcgtg cttctttttg
     6541 ttcatcatca gctaaaatct tcgtatgagc atagtatttg ttgaatgctt gtgctaattt
     6601 gattgcatgt ttcgcaatca ctgacggttc gtatttttcc ccagcagaca agaccgtttc
     6661 agggtatttt tgaactaatt taaccacttc ccagctggta tcgtcattca atgcatagtc
     6721 tgctgtttct gaagggacaa atcctgcttt ttccaacagg ctgactgcac gagcatgtgt
     6781 atattgcaca taaggtccag tttctccttc aaatcgaaca acttcttcca ggttaaagtc
     6841 aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa ccccaacttg
     6901 tttagctaca gcatctttgt tttccaaatc agggtttttc tcgctgattt gttctttagc
     6961 agattggatt gcttcattta atacttcttc taataagacg atctttcctt tacgagttga
     7021 taatttttta ccaccttgcg tgatcaaacc aaatggaata tggtgcatgt cgtcagacca
     7081 gtcaaagccc atttccttca agacagcttt caactgttta aaatgatagc tttgttcatt
     7141 ccctactaca tatagagatt gtttgaagtc gtaagtacgt ttacggtaca atgcagcagc
     7201 taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg ctggattcag
     7261 gtcataagca gataggtcaa cgatctctgc acctttatcc tcgttcagca aatgtttttc
     7321 ttctaaaagt ttgacgattt catccatctt gtcattgtag aaagcttcac cgttcaacga
     7381 atcgaaacgt acttccagca gatcatagat tttattgaat tctttcatcg attcatcacg
     7441 gaaccattgc cataattgaa tagcctcttt atcgccttct tccagacgtt tgaaccaagc
     7501 acgagcttct tcatttaatt ctggttctgt ttcagctact tcatggaatt ggacgtataa
     7561 acgaagcaat tcattgattg gttctgcttt aacagcttct tctgtacccc attttttgta
     7621 agcgacgatc agttttccaa actgtgttcc ccagtcacct aaatggttga tgcggattgg
     7681 ctgataaccg attttttcca taatgaaacc gatcgaatta ccgataactg tcgaacgcaa
     7741 atgtcccatt gagattggtt tggcaatatt aggagaagac atgtcgattg gtactgttcc
     7801 ttgattcccg atgttgctat ccccataatg ttctttttct ttcactactg tttgcaaaac
     7861 ttttttgctg ataagttctt tattcatgaa aaagttcaag taaggtccga caacttcgat
     7921 tttttcaaag ttggcagaat cgattttttc tgctaaatct gcagcgattt gctgtggcgc
     7981 ttttcggtaa acttttgcta atgaaaaggc tggaaatgca acatctccgt gttcagctga
     8041 ttttggattt tctagtaatt gctcgacttg ttccagtgtt aaatcatctt tgacagcgtc
     8101 atagacagct tttgcgacaa gatctttatt attcatcttt attcctccta aagtgttccc
     8161 ttgattgtta aacaaaaaaa atctctagcg acggcatact tagtatgcca tcactagaga
     8221 cgagatttcc cgcggtacca ctccaattgt cctaaaaaag gaccagctcc atctgatatc
     8281 ggtagacgac cgtctgttta aaacagaatt ctccaagtgc gcttcgttgt atcttcttca
     8341 ttcggcttcc accacttccg aattcgctag ccaagaatga tacaagtact ctcttgttca
     8401 acaaatctta gtcttattaa actttcttga attatatcag aaaaaaaaac gaaaggctag
     8461 tcgtttcata aaaaaacaat ccagatggcc taaaggagaa gaaataccac ctggatagct
     8521 ataaatcatt tcgtaaatac gaaagactta acaaattaat cagctacgat aatcgttcct
     8581 tcttcagaag ctaaaagatt ctcgatattt tctaaagaag tgatgattgc cttagcgttt
     8641 ggttttgctt caacaaatgc aatagctgct tctactttcg gaagcatgct tcctggagcg
     8701 aattgctttt catcaatgta ttgtttcatt tctgaaacag taacagtttc taatttcttt
     8761 tgatctggtt tttggtaatt gacatacaca ttgtctactc ctgtcaatac gatcagcaaa
     8821 tccgcatcga tgatttcggc taatttttct gaagcaaaat ctttatcgat tacggcttct
     8881 cgtccttcta atccagtagc tgtttctaca acaggaatcc caccgccacc aacagagact
     8941 gtgataactc cttgatcgac cagcgtttcg atgacacgtg cttctttaat cgagatgggt
     9001 ttaggggaag cgactacttt tctccatcca cgtccagcat cttctttgaa tgtcacagtg
     9061 gagtcagcat tcatttgttc tttggcttct tcttccgtgt agaatggacc gactggttta
     9121 cttggatttt tgaatgaagg atcattttca tccacaataa cttgagtgac taaagaaacc
     9181 acatctttat cgatcccttt ttccttcagt acttcgccca tggcgttttg taaccagtaa
     9241 ccgatagatc cttctgtcat tgcaacacat gtatctaaag gcattgccgg tgttttctca
     9301 gaatcagcag cttgctgctg aatcaatagg ttaccgactt gcggaccatt tccgtgggag
     9361 atgatcaatt catctccttg ttcgataaat ttcactaaat actttgctgt ttccattaac
     9421 gcttcttgtt gggcttttgc acttgcatcc gtagacaaga tggcattgcc ccctaaagcg
     9481 acaactactt ttcgtttggc catttcattc gtcctttctt cagttctacc gaattttcta
     9541 gataacaaaa cgagccagcg ttttgtgatt ttccctcaat gctgactcgt tttttcgtga
     9601 gatgaatgtg gttatgctta aacgcgaggg ataaacaagt tacctaatgt tgctgccatg
     9661 atcgctttga ttgagtgcat acgattttct gcttggtcaa attgacgagc atatttgctg
     9721 cggaagactt catctgttac ttccatttca gtgatgccaa agcgttcttt catttgttca
     9781 ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt gcaagaagat caaacgatct
     9841 gtgttgtgtg ttttttctac catttccata ttgatttggt aaggtttcaa caatttgata
     9901 cgttcttcga atttatcttc ttcacccatt gatacccata catcagaata aaggacgtct
     9961 gctccgtcta cacctttatc aacatcgtcg gtaatcatca attgtgcacc ggatttttca
    10021 gcaaagcctt ctgccatttt gacgatttct tcttcaggtt gtaattcttt tggtgcaacg
    10081 atacgcatat tcgctcctaa aattgcacct gtgactaata aagagttagc catattgttt
    10141 cgaccatctc cgcagtaagc aacagtgata ccttctactg ttccaaagtt ttcttggata
    10201 gttaaaaagt cagcgatcat ttgtgttgga tgccattcat cagtcaagcc attccaaact
    10261 ggaacaccag agaattctgc tagttcctct accatttttt gactgaatcc acggaattcg
    10321 attccgtcaa acatacgtcc tagtacttta gctgtatctt ctgtggattc ttttttacct
    10381 aattggatat catttgcacc tagatattca ggatgtgcac ctagatcgat cgctgcagta
    10441 gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata aaagcgcaat attttttcct
    10501 tctagatagt gatgtgggat accacgtttt ttcaagtctt tcaagtgttc tgaaaaatcg
    10561 atcaaatatt gtaattcttc ttttgtaaag tctttttctg ctaataagct tcttccttgg
    10621 aatactgatt ctttcatatt aaacattctc ctttagattg tttttattta agattaaaga
    10681 ggaaatcccg acggcagctg acagtaagac tgccgtcagg aaacaaatta cttcaagtct
    10741 tcacggacaa gtggttggct catgcagcgt ggaccaccac gacctcgtga caattcactt
    10801 gaattgattt ctaacacttt gatgccatag ctacgaagca attcgttcga tacataattt
    10861 cggttataag tgactacaac acctggagcg attgccaatg tgtttgatcc atcattccat
    10921 tgttctcgag gtgcaacgat ctcatctcca ttaccagttg ggatcagtac aagatcatct
    10981 aatccaagag cagctttcat cgtttcgtgt aagttatctg aatgagtaat cttgatatca
    11041 tcaccatctg gttcgattgt aaagacatcc atcttaccgt ctttactttg aattgctgga
    11101 tggatagtga atttatcgtg atctaccatt gtaaatacgg tatccaaatg catcattgca
    11161 cggttgttcg gaattttgat tgccaacact ttttcaaatc ctgaattttt tgcgaatagg
    11221 tggcgtgcta atttttctaa agctttcgca ttagttcgtt gagaaatccc aacagctact
    11281 actttatcgc ttaagatcaa ttcgtctccg ccttcgatat gatctggatt ttcacgatcc
    11341 aaccatactt cgattccttt atttgcaaat cttggatgat gtttcaaaat atattctgta
    11401 aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat tgactgtcat cccatttccc
    11461 attgatgcgg aagggtcgcg tgtaaagtac aagtttggca ttgggtccat gtaaaatgga
    11521 tagtcatcgt ctgctgacag atcatataga ctgctagaac gtacttcgat atcttttgta
    11581 cgaaccccag ccatgatttt atctaccatt tcttgcgttt ccattgatag aaggaactca
    11641 tgaagaccct ctcttacagc attcgatgca atatgagatt catccaacat tttattcaag
    11701 aattgttctt tgacattgcc tgcgtcaatc gcttctgccg ctaatttttc caagtaaaga
    11761 gtctcgacgc cttcattttg caatgtcttc gcaaagttgt catgttcttc ttgagcaatc
    11821 ggaagatatg gaatatcatc gaacaacaga cgatccataa tatctggtgt caaattttcc
    11881 acttcttgtc caggccgttt cagtaaaact gtctttaatt tccctatttc agagaaaacg
    11941 tgaataggtt tatccatgtt gtgttgcctc cttctttttg attacatgta taggatatcc
    12001 tccttttgta atcactttca taattttgtc tcattcaaaa tgagacattc ctcacattaa
    12061 tattcgataa ttaaataatt aaacgttttt ctatttttaa gcgtaattta ttc
//
LOCUS       DAYYAO010000083        12021 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000083 DAYYAO010000000
VERSION     DAYYAO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.086"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            560..1531
                     /locus_tag="ACHARI_002218"
     CDS             560..1531
                     /locus_tag="ACHARI_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8443238.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1508..1597)
                     /locus_tag="ACHARI_002219"
                     /pseudo
     CDS             complement(1508..1597)
                     /locus_tag="ACHARI_002219"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(1609..1845)
                     /locus_tag="ACHARI_002220"
     CDS             complement(1609..1845)
                     /locus_tag="ACHARI_002220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8443239.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1814..1945)
                     /locus_tag="ACHARI_002221"
     CDS             complement(1814..1945)
                     /locus_tag="ACHARI_002221"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8443240.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2021..2404)
                     /locus_tag="ACHARI_002222"
     CDS             complement(2021..2404)
                     /locus_tag="ACHARI_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8443241.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2437..3222)
                     /locus_tag="ACHARI_002223"
     CDS             complement(2437..3222)
                     /locus_tag="ACHARI_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8443242.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3400..4500
                     /locus_tag="ACHARI_002224"
     CDS             3400..4500
                     /locus_tag="ACHARI_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8443243.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4490..5314
                     /locus_tag="ACHARI_002225"
     CDS             4490..5314
                     /locus_tag="ACHARI_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM8443244.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5311..6126
                     /locus_tag="ACHARI_002226"
     CDS             5311..6126
                     /locus_tag="ACHARI_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8443245.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6123..6953
                     /locus_tag="ACHARI_002227"
     CDS             6123..6953
                     /locus_tag="ACHARI_002227"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8443246.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6960..7991
                     /locus_tag="ACHARI_002228"
     CDS             6960..7991
                     /locus_tag="ACHARI_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8443247.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8098..9371)
                     /locus_tag="ACHARI_002229"
                     /pseudo
     CDS             complement(8098..9371)
                     /locus_tag="ACHARI_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9598..9783
                     /locus_tag="ACHARI_002230"
     CDS             9598..9783
                     /locus_tag="ACHARI_002230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8443248.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9808..10527
                     /locus_tag="ACHARI_002231"
                     /pseudo
     CDS             9808..10527
                     /locus_tag="ACHARI_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(10525..11045)
                     /locus_tag="ACHARI_002232"
                     /pseudo
     CDS             complement(10525..11045)
                     /locus_tag="ACHARI_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            11333..11986
                     /locus_tag="ACHARI_002233"
     CDS             11333..11986
                     /locus_tag="ACHARI_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8443249.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         3481 a         2207 c         2734 g         3599 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcgaataa
      361 gggaataaac caataaaaaa tgatttagaa tattaaaata acaaaaaaga ggactacctg
      421 tattagtcct cttttttgac cagattttga ccagtctatt ttacatccta gtatatttat
      481 atgagctaat tttatgatta aatggtaagt ttttccttgt gtgttttagt ttaaactagt
      541 gtgtaattca aaaatctata tggaaggaac agtattaaga atagaacagg gttctctaca
      601 tgatggtgct ggtttacgga ccgttgttta tttaaagggc tgcccattac gctgtgcctg
      661 gtgttcgatt cccgaatcac aaagcaagca gattgaaaaa ggatttggcc agaccatgac
      721 cgctgaagag gtaatggatg agattgagaa ggatgctgtt ttttactttc actcggatgg
      781 cggagtgacc attagcggag gcgaggcact ggttcaggcg gattttgcta aagaaatttt
      841 gcaaaaatcg aaatacattg gaatcaatac cgtactggaa accagctttt gtggtgctta
      901 taatgaaatc caaaaggtag ctccctatgt ggatacattg tttgtggatg taaaaatgtt
      961 taccagcaag ctccacaaac aatggaccgg cttagataac caacaaattt tgaagaatat
     1021 tcgtcgcttt ttaattgagt atcctaactg cgaagtgcga attcgagttc ctgtggttcc
     1081 aggtatcaat atgaacctga ccgagctgct gacgattgct tgttttgtgg cagatcttga
     1141 tcggttcgta ccgttagaat tactaccgta ccactgctat ggtatgcacg gttaccaagc
     1201 attgggactg gagtatccgt tggcagacac gccagcgcct tcacctaaag aaatgtttgc
     1261 attggcagat caactggctc gcacggttcc tcatttgctg gtcacagctt taaccaaaac
     1321 atttatttat ttattgaatc caagctttat cttatggata aatgaaagac actcactagc
     1381 tgcagccagc tcaagaatag agcttttgct gaagtcagtg agtgtctttt tacgatgttt
     1441 tttcgaagtt aatttgtcga atgaacactg gctcaatcgg tacatcttca ctgtttgtcg
     1501 gctgagctgc gatcgctgct acgatttcta acccttggta tacctgtcca aaaacagtat
     1561 aacgaccatc cagctccggt agtcctccaa gctccaagta tgcttgcttt acattagcag
     1621 gtcagcctgc gcctttgtgt tttgcacaat aaaaaactgg ttgccgttgg tgtgggggcc
     1681 gtggtcccct aaggccaagg cgccattaaa atgataaagt ccgcgatgca cttcatcagc
     1741 gaaaaagccc tcatagctgg aaacagcttc atcaccagaa ccatccaaag cgccaccttg
     1801 aataacaaat tccttaatga cacgagccga aaggtgtgtt gtcataaacg ccttgctcag
     1861 ctagggagcg ccagttggct acagccaggg gagcttggtc aggaaataag cggagacgaa
     1921 tttcaccagc atcggtgacc agcacacaaa cttcttcctg ctgcggtttt tctaattgaa
     1981 agagaggaag gtttgcaact gaaacgcctt gatcctgcca tcaaatatcg aaaatccgcc
     2041 actcgacctt cgcctgatca ccaaggcgag ccattcccaa taaaacggta taggcggttt
     2101 catccgcttg aaaggtcaat tgcagctgtc ctcgtaaatc gttgtagtca tctggggcgg
     2161 ctacttgata aaaaagatga aactgcgcat gcaaatattc caaaggctga tctttaaagg
     2221 ctgcaaaata agcctgaact ttttcccgct gcaagctgtc agccaccaaa aaattttcca
     2281 gttgttgttt tggaacggct gctaacaagg atcggcaaac cgcctcaatt ttcaacgcat
     2341 cccaatgtcg cgcaattaaa taatggatgg gtgcctcttg attgtagtcc cacaggttgc
     2401 tcattttttt caccactttc ttctattata taggaatcac aaaatcggca gcggcgtcag
     2461 gcggcgttct tttccttgaa tgactagcaa cttttctacc tgacagtctg ccaacagctg
     2521 actcattttt ttaaagccca gctgataatc ttcggcaaca tgagcatccg agcctaaggt
     2581 aaagagcttt ccgccgacac tttgatacaa aggaacggca taacggtaaa ggtcttcgtt
     2641 gccgtatttc aagaagctct tggcatttaa ctccatcgcc aattctcgtt caacaacctt
     2701 tttgaaaatc gttagcagct gagcctcaaa atgctccgcg aattcttccg ctgtgaaggt
     2761 gaagcggcgt aatccataat caaagtgagt caaaatatct ccttctggaa aatccgtcaa
     2821 gacctgcaac atctgatcaa aatattcctt ggtagtttga atgggatctt tggttaaaac
     2881 cacatcgtcc atataatcaa agcggccatt ttgatgaata cttaccagct ttaaatcata
     2941 aggatgctga gcgaggtaat ccacgatccg ctcttcttgt cctggaacaa cgccaatctc
     3001 gatacctcgc agaaatttgg tggaataggt ttcttttaaa gagtccagtt tgtttatata
     3061 ggctggataa tccggaatgt catcttttcc ggtagatgga ttgtttaaat caaagtgatc
     3121 cgtagaaacg aaaaactccg gctgataggc taaatagttt tcaaattttt cctctgaatc
     3181 aaaggaaaaa tacgtgtgga gatgctgatc ataatactgc atagggagcc tccttttttc
     3241 tactatcata ccgcaaaagc tttagaaggt taacgggaat tcgtcgaaat ttacaaacca
     3301 tatacaacct ttacattgtt catgaaacct tttcaagaaa ctgattctct cattacaaag
     3361 ctttgttaga atggaattat cgaaagggga gaagaatgaa tgaaaatgaa tgaaaaaatt
     3421 cgtaacatga ctatcggttt attttgtgca gcaacagtaa gtatcctgct gacaggctgc
     3481 ggctcaggtg aagcaacgaa gcaatcggct gcggcagacg actctttaga tacgattata
     3541 gaaaaagcca aggaagaagg agagattgcc agcttgggaa tgccggatac ttgggcaaat
     3601 tgggtaggca cttggaacga ccttgaaaaa gaatacggct taagtcacgt ggatacggat
     3661 atgtccagtg cagaagaatt ggctaagttt gaatcagaag gaaaaaatgg aaccgccgat
     3721 attggtgatg tcggtatcag cttcggtccg ttggctgaaa gcaaagagtt aaccttacct
     3781 tataagacca actattggga ggaaattccg gattgggcaa aggatgatga tggggattgg
     3841 ttgttaagct atacgggaac catttccttt atcaccgata aagagaatgt gaagcaagca
     3901 ccgactagct ggaaggagct gcaagagggt gactatcaag ttagcgtggg ggatgtgacg
     3961 attgccaacc aagcacaatt tgctgtattg gctgcggcga ttgccaacgg aggtgacgaa
     4021 aaggatattc agccaggtct gaattactac aaaaaaatgg cagaaaaagg ccgtttgtcc
     4081 accgttgatg ccagcgtagc aaatcttgaa aaaggtgaaa tcgatgtggc agttgtttgg
     4141 gacttcaatg gactaaatta tcgtgaccaa attgactccg agcgttttga ggtaactgtg
     4201 ccaacagacg gctccatcat tagcgggtat acaaccttaa tcaataaaaa tgcccctcat
     4261 cccaatgcgg caaagctagc acgggaatat attttatctg atgaagggca aatcaatttg
     4321 gcaaagggct atgcgcgacc tattcgcaag gtagagctgc ctaaagaagt agctgaaaaa
     4381 ttactgccgg aggatgctta tgaagcggcg gtgccagttg aagacaatga agcttggaac
     4441 aagacctctg tagagcttcc agagctttgg caggcggagg tactgaccta tgcaaagtag
     4501 acttgacaaa ctgattgtag ttatactaga cggctgtcgc tacgacacag ccgtcgccca
     4561 gcttgggttc atgaatcatt tagttgagca ccagcaagca agcttattga aggtaaaaag
     4621 tgagctgccc agcaattcac gtccgttata cgaagtactg atgaccggcg ttccagccta
     4681 tgaaaatggc atctatagta attaccatgc tcagcgatcc aaagagtggt cgctgtttga
     4741 attagttaag gaagctggcg gaaaaacagg agccgccgct taccactggt acagcgagtt
     4801 gtacaatcaa gccccctata atcgtttggt tgatcgcatc cagcatcagg aggagcgact
     4861 gattcagcat ggcatctttt atagtgagga cacttaccct gattcgcatt tatttgcgga
     4921 tgcccattat ttagtgaatc agtatcagcc agactttttg gtcgtacatt cgatgaatat
     4981 tgatgatgtc ggtcacaaat ttaccgccga ttctcatgaa tatgcagcag cggttaatca
     5041 ggcggacaga ttattggcta tttaccttcc tgaatggctg caaaaaggtt atcaagtcgt
     5101 ggtgactgcg gatcacggga tggatgcgca cggactgcat ggcggcagct tggctgcaca
     5161 tcgtgaagtt cctttttatt tgatttcaaa taaacgccaa aagctgaagg aacaaactgt
     5221 accacagctg ctggcggctc cattgttttg ctggttatta ggaattgcac cgtcagagaa
     5281 aatgcaggac attcaagaat tgatgagggg atgaaagaga tgaaaaaacg cttggttctg
     5341 gttcctgcct cctttatctt ctgtgcattt ttactgctgc cgctgctttt tatggttctg
     5401 tgcagcttgc acagtgattt aactaaccag tggacattgg ccaattacag tgatattttc
     5461 agcaacaaat attacacaca ggcctttgtt aacagcgtac tgattgctgt cagctccagt
     5521 gtgattgggt taatcgggac gtttatttta tgtttatgct taatgaatct atcgactaaa
     5581 ttgcaggaga aaatcacctt tgtttccaat ttggcagcta attttgctgg gattccgctg
     5641 gcattctctt ttattatttt gttggggaat gtaggtgtgc tgaaaatgat tttgccattt
     5701 ctagcggact tcaatttgta ttcttggtgg ggattgacca tcacgtatac ctatttccaa
     5761 attccgttag gggtcttgtt tttgtatccc agcatgaagg aagtgaaaaa ggaatggctg
     5821 gagtccgcac aattgctggg cgcttcatca gcgtatgctt ggttcaaggt ggctttgcct
     5881 tttttgcgac catcgattgc cagcaccttt gtcatcttat ttgctaatgg catggggacc
     5941 tatgaaacag cgtatgccct gaccagcagt aatgtaaatc ttttaacgat tcgaattgcg
     6001 gctttagtgt cgggagatgt gttcgccaag cctaatttag gcagtgcgtt ggcagtcgct
     6061 ttaggtgtcc tgctggtaac ggcaatgttg attattcaaa agaaaggcaa ggtggtgcag
     6121 acgtgaaaaa gttaataaaa ccgattttgt tctgctttgt cctgtattac ttgctgccga
     6181 ttattggaac gattttgtat gccagctcca ctaaatggtc aaaatcgctt ttacctagtg
     6241 attttacctt gcaatggttc caacagctgc tgacggacag ggaatttatt gctgcggtgg
     6301 gtcgctcact tttattagca ggtgtggtct tggtgacgat tctgttgttg atgattccca
     6361 ccattatttg gattcatttg tatttccctc gtttgaatcg ttggctggaa aagctgctcc
     6421 tgctgccgta tgctttacca ggagtgattt tggtaacggc tttgcttcga acgtatgctg
     6481 aaactggtat cccaatgttt gtagtgttgg taggtgcatt gtttattacg gcgttgccga
     6541 ttgtctatct tagtcttaac aaccagatgc gcttaattaa tctgaaggaa ttagtggatg
     6601 cagcagaaac cttgggcgcc ccgatgtcta cgattattat ccaggtgctg tttccaaaca
     6661 tccgtatcgg tgtgacccta gtttctctga tgattttttc ttcggttttt ggggagtaca
     6721 tgctgacgaa cctgttgatt ggcggtcgat ttcagacggt acgcatctat atgctgcggc
     6781 ggatgaacga aaacggccat ttggctagcg ctgtgatggt tttgtatttg ctgtttatgg
     6841 tgggaattgc tttaactacc tttatgctga cttatcgaca aaagcaacag ctgtccctta
     6901 agcagacgag ggaagagaag cagtcgaaaa agaagccctt aaaggagttg tgacggaaaa
     6961 tgtttttaga aatacatgat ctcaatctgt tttttgatga aaaacagatt ttagctattg
     7021 atgaaattca gattgaaaag ggtgagctgg ttactttact aggtcccagc gggtgcggaa
     7081 agtctacgtt gctgcgctgt atcacgggat tggaacaacc aaagagcggt gagattgtgc
     7141 tggacaatga gaatattgcc gataagccta cgaaggatcg caatatcggc tttgtttttc
     7201 aacagtatgc attgtttcca actatgactg tttttgaaaa tgtagccttt ggtttgaagg
     7261 taaagaagct gagtcctgag gtgattcagg aaaaggtttt tgagatgcta tcattggtgg
     7321 atatgacgga gcaggcagat aaaaatgtcc agtttctttc tggcggtcaa cgacaacggg
     7381 tggcactggc tcgctcattg gtcactgaac cgaaagtcct gctgctggat gagccattaa
     7441 gtgctttgga tgcccgaatt agaaaacagc tgcagcggga tttgcgcgct atccagcaat
     7501 cgctagggat gacgatgatt tttgtgactc atgaccaaga agaggcgatg cgaatcagtg
     7561 atcggatttt tgtgatggaa gctgggcggg tggctcaggt ttccacctcg aaggaattgt
     7621 atcagaatcc tcagagccga tttgttgcgg aatttatcgg caactataat cgtttcgaat
     7681 ggtatgagct gaatcagtat acccacgagt tttccgctaa gggcaatcgc tgcatttatt
     7741 atttaagacc ggagctgatt caatttgaac cggttcctca gggggttaag attccggttc
     7801 tatggaagga atcatttatt ttaggcaaca ttcaacggta tgtttttcaa acgctggagg
     7861 gcaaagaaat tttagtggat cggttgaacg atcgggaaga gaaaattagc gatgccttat
     7921 acattcagct gaaagatatt ctagcaatcc ctgtggaaat tgatactcag cattttgcac
     7981 aacaaagcta gtgtatagtc ggcaaacatt tctggctctt tgtcaaatag gactgataga
     8041 gtgatataag aacatctaag gtagaattga ttttctgctt tagatgtttt tttatgttta
     8101 ataagtgatc agatgattga ttaagaaaat tctggttctc atcctaaccc gtaagctatt
     8161 cgtttcaatg ctttgatgtg ggtatgcaga ttttctattt tgccatttga gtaaggcaac
     8221 tctaaggcta aacgaatgcc ggattcatag tttataaacg ctttttttat atgatgcagt
     8281 tctcttggaa ccgacttggg cattgttttt acaagctgaa agaagatgtc cgcttctttg
     8341 tcataaaaag cttgaagaat ttcttgatag taagcataag ctattcgtag ttcatcagaa
     8401 gtagaaagta gacgttctac gacttcactt tcggttaaat aggcccaacc aaaattacgc
     8461 cattttgtcc atttatcata aacaagcgtg tcctgtcgtt tgatcagaag tcgagataaa
     8521 gccttcactt gtcgatagtg tttggcttgt attggatcgt ttcggtcgaa gcttttcatg
     8581 actcatagtc aaatatcctt aaaactgcga atgaggtgct tgacgatgtg aaagcgatcc
     8641 acaacgagtt tggcatgagg aaagcactct tttatcagca aagcataaga ggcgttcata
     8701 tctgttacga gtatttttac gtttaaacgt gcacgtcgtt caaattgcat aaaataagaa
     8761 atgagatctt tcttcttacg tgaaggaaga atatccagta atttcccagt atcgccatcc
     8821 atacaagaaa aagaataggg accgacttga tttttcaatg aacgaaattc atctaccaat
     8881 gaacagcgag gcaaccaatt tattttcacg tttgtcatgg ggagcagaga agcgagtgtt
     8941 cgcgaaacgg ttgaccaaga aacgccgcaa tgtttagaaa tcgtcgtcat tgcttgtttt
     9001 tcagtcaatt cctccaatat tttataatgt acttgtttgg cgataaaaca gtttttttca
     9061 acaatgtagg tattcgccgt aaaataggtg tgacagccac gacagaggta ccgttgcttt
     9121 gccaaagcaa gataagtccg ttgattcgca cacttattga gaaggaccag cgattttttc
     9181 tttccgttct taacgatcag cccatgttga ttcacctgtt tacactgagg acagggctga
     9241 atcgttttaa tgagcgtggc agaaacaacg atactgttga ttgaatgtat ttttttcttc
     9301 tcgacatgag tgatttgaat gtttccatct ttgattccta gtaatagttt gataagatga
     9361 agatcaggca tacagaacgc tcctttgatt gtgttgtggt gattcaattc taacggagct
     9421 tctgtatgtt ttgttttttt atgctcaaaa ggactgatag aatgatttac tatcagtcct
     9481 agaaattata gcgccacatt tctttcacct ttgacttttc agacggcagt ttttatgctg
     9541 atactgtaga gagagcagga cacagctttt gcttgttttg ggtaaggggg aaagaagatg
     9601 accaaagcac ttatttttga ttttgatggg acgatgattg acagtgaatg gatgttttac
     9661 gagagtattc atcactattt actgaagcct atttactgga gcagttcggc tataaaattc
     9721 cttttgcaga ctatcaacaa ggtgttggga cggcagcaga actgtccttt tatgaaggga
     9781 taagtcaagc ggttgggaga ccagtggatg ctgaacattt tgagcagcgg atcagcagca
     9841 ctggaaaatc ggagtagtct caattctgaa ctgcgggagc tagactattt ttttgaagca
     9901 catccgatga ttaaggaaaa aattgattcc tttgtgacga tcgatgatct tcaggtcggc
     9961 aaaccagatc cagaaggtta tttgttgtgc ttgaaacggc tgagggttga acaggctgtt
    10021 gtagttgagg atagtccagt cggtgcccaa gcagcaccgc atatgacagt tgatgtacaa
    10081 gcgttggatg cagatttttt cgcctttagc gggcataaaa tgtgcgggcc gacaggaatc
    10141 ggtgtattat atggcaaacg tcaattgtta gaacaaatgg agccagtcga attcggcggt
    10201 gaaatgatag attttgtcaa tctttacgat agttcatgga aagagctgcc atggaaattt
    10261 gaagctggta ctccgaacat tgcaggagca atcgattact tgaatgagat tggtatggag
    10321 aatattcatc gttatgagca agaactggtt gcctacgttt tacctaaatt acaagcgatc
    10381 ggcggtatca ccatttacgg accgcaagat cctgaacatc atactggcgt ggttgctttt
    10441 aatttggatg gcatccatcc ccgtgatgtg gcgactgctc tggatatgga aggtgtcgct
    10501 gtacgttcag gacaccattg tgcacaacgg ttcatcgacg atttctgttt gactaattgg
    10561 tcgtttaaac agtcgatgat aatgccgttt gagcggctcc aatgtcgtgg aatctttgag
    10621 taagagtttc caatatcgct tgagccgacg gtacttcttc tgattttcag attacgtgat
    10681 tgattttcgt acagttctat gagaatcgcc tgtttcagtt cctttgttag ttgatgatgt
    10741 tcatcaacta agtccgtttt cgcagaaaag gtctgcccac aatcatagca tagaaaacgg
    10801 gtacgtttta attctaaaat agtcaacttt tctttgactt tcaacatctg agtacgtgtc
    10861 gtataggaac cgttgcggat aatacgaatc gatccacagt gttcacaatg ggaaggtgag
    10921 tcatccaatc tccctttaat aaactgcgct tgaacgcctt tatattcttt ttcaatcagc
    10981 cattcttcat caaaaaataa atgtttatct gttaatccga gtaattttct agtatgatta
    11041 tccatagagc gactcctttg gtagggtgat gttttggtcg actaaatttt accaaaagtg
    11101 agtctctttt tgagttgaaa cgaagaaaaa gtgttagtag aaaatcgcct tcgattttcc
    11161 aacaacacaa aatattatag aagccttttt tctaaaggat ttcaaggtgt ctgtttgcat
    11221 ttattaaaaa aaagaaaaag tattggcaac agaaggcgtc accaacacta gatattatag
    11281 agccataata tgctgtataa tatctagtgt ataggcttaa gtggaggtat atatgaaaat
    11341 attaactgtt gaagatgata atatgataag agagggaata agtgaatatc tttcagaatt
    11401 tggatatact gttattcaag ctaaagatgg aagagaagcc ttgtcaaaat ttaatagcga
    11461 tataaatttg gttatcttag acattcagat accttttata aatggtttag aagtgttgaa
    11521 agaaattaga aagaaaagca atttaccaat tctaatcttg actgcattta gtgatgaaga
    11581 atataaaatt gatgcattta ctaatttagt agacggatat gtagaaaagc cattttcatt
    11641 gccggtctta aaagctagaa tagattcttt aattaagaag aattatggac atttagagaa
    11701 gtttgaatat aaggatctat cggttaattt taatagctat acagctaaaa taaatggtga
    11761 agaaatagat gtaaatgcaa aagaactaga agtattaaaa tgtttattgg ataataatgg
    11821 acaagtgtta acaagaatgc aaattattga ttatgtatgg aaagatagcg aagaaactcc
    11881 ttatgatcga gtaatagatg tatatataaa agaacttaga aaaaaattac aattagactg
    11941 tataactact ataagaaatg taggatataa attggagaga aaatgaaaaa attaaagata
    12001 tttccaaaaa tgttcataca a
//
LOCUS       DAYYAO010000084        11802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000084 DAYYAO010000000
VERSION     DAYYAO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.087"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..195)
                     /locus_tag="ACHARI_002234"
     CDS             complement(<1..195)
                     /locus_tag="ACHARI_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443250.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="ACHARI_002235"
     CDS             complement(211..825)
                     /locus_tag="ACHARI_002235"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8443251.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="ACHARI_002236"
     CDS             complement(972..1655)
                     /locus_tag="ACHARI_002236"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8443252.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="ACHARI_002237"
     CDS             complement(1764..2222)
                     /locus_tag="ACHARI_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8443253.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="ACHARI_002238"
     CDS             complement(2306..4069)
                     /locus_tag="ACHARI_002238"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8443254.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="ACHARI_002239"
     CDS             complement(4290..5750)
                     /locus_tag="ACHARI_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443255.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACHARI_002240"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACHARI_002240"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8443256.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="ACHARI_002241"
     CDS             complement(6907..8334)
                     /locus_tag="ACHARI_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8443257.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="ACHARI_002242"
     CDS             complement(8365..9579)
                     /locus_tag="ACHARI_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8443258.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9665..9892)
                     /locus_tag="ACHARI_002243"
     CDS             complement(9665..9892)
                     /locus_tag="ACHARI_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443259.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9920..10162)
                     /locus_tag="ACHARI_002244"
     CDS             complement(9920..10162)
                     /locus_tag="ACHARI_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8443260.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10194..10751)
                     /locus_tag="ACHARI_002245"
     CDS             complement(10194..10751)
                     /locus_tag="ACHARI_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8443261.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10764..10952)
                     /locus_tag="ACHARI_002246"
     CDS             complement(10764..10952)
                     /locus_tag="ACHARI_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8443262.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10965..11531)
                     /gene="amaP"
                     /locus_tag="ACHARI_002247"
     CDS             complement(10965..11531)
                     /gene="amaP"
                     /locus_tag="ACHARI_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8443263.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3460 a         2447 c         1934 g         3961 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtatgat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca tataaaatat
     9841 ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc atttcaacat
     9901 aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact aatactaaaa
     9961 tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt ccccaagttc
    10021 ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt gcgatgatcc
    10081 ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata ccgccgacga
    10141 ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac ttcttattgt
    10201 acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc agattttgct
    10261 ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt tactttgtct
    10321 tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc aacattcaca
    10381 ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat caattctttg
    10441 atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc tagatccaca
    10501 gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc tgtgttgact
    10561 aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc atctacatct
    10621 ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa ttcaccattc
    10681 atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt attcatgttt
    10741 ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat tcctgactgt
    10801 ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa caatgtcttg
    10861 aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc aaagataata
    10921 gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc cactcgagag
    10981 tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac tgccactttg
    11041 accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg catcaataca
    11101 cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg gatatggatt
    11161 cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc ttcgtgtaaa
    11221 tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc atcttcccgt
    11281 ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa gatgataata
    11341 gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta gtaattcgtt
    11401 aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc attgatcaac
    11461 gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat tatttttaac
    11521 cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa ctctaccgac
    11581 aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg ctaccaaaca
    11641 ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa ttgcttactt
    11701 attctctatt tacttttgat tgctggctat acttaaagga tattattcaa gattaaaaat
    11761 tgcaaagatt atgctcagtt attattacaa gattctattt at
//
LOCUS       DAYYAO010000085        11624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000085 DAYYAO010000000
VERSION     DAYYAO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.088"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..495
                     /locus_tag="ACHARI_002248"
     CDS             289..495
                     /locus_tag="ACHARI_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8443264.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(697..1698)
                     /locus_tag="ACHARI_002249"
     CDS             complement(697..1698)
                     /locus_tag="ACHARI_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8443265.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1703..3616)
                     /locus_tag="ACHARI_002250"
     CDS             complement(1703..3616)
                     /locus_tag="ACHARI_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8443266.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3784..4290)
                     /locus_tag="ACHARI_002251"
     CDS             complement(3784..4290)
                     /locus_tag="ACHARI_002251"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8443267.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4450..4890
                     /locus_tag="ACHARI_002252"
     CDS             4450..4890
                     /locus_tag="ACHARI_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8443268.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4916..5944)
                     /locus_tag="ACHARI_002253"
     CDS             complement(4916..5944)
                     /locus_tag="ACHARI_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443269.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(5999..6073)
                     /locus_tag="ACHARI_002254"
                     /pseudo
     CDS             complement(5999..6073)
                     /locus_tag="ACHARI_002254"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6076..6432)
                     /locus_tag="ACHARI_002255"
     CDS             complement(6076..6432)
                     /locus_tag="ACHARI_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443270.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6730..7704)
                     /gene="sgrA"
                     /locus_tag="ACHARI_002256"
     CDS             complement(6730..7704)
                     /gene="sgrA"
                     /locus_tag="ACHARI_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8443271.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7900..8739)
                     /locus_tag="ACHARI_002257"
     CDS             complement(7900..8739)
                     /locus_tag="ACHARI_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8443272.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8924..9811
                     /locus_tag="ACHARI_002258"
     CDS             8924..9811
                     /locus_tag="ACHARI_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8443273.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10405..11099)
                     /locus_tag="ACHARI_002259"
                     /pseudo
     CDS             complement(10405..11099)
                     /locus_tag="ACHARI_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11083..11481)
                     /locus_tag="ACHARI_002260"
     CDS             complement(11083..11481)
                     /locus_tag="ACHARI_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8443274.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3469 a         2310 c         1742 g         4103 t
ORIGIN      
        1 gattgttatg taaaataaaa aagtttagga tacatcgaat ttttggggaa attcttttgg
       61 gggtatccta aatcttttct attatattcc catattttct attattggtc tagacgtatg
      121 atggtaaact ttgtacctat ctgattgctg aatcattaat tgcttatcat tagccatctt
      181 tgtttcaagt tcgctagatg atcattttat tcacatccta acaaacttgt cacatttata
      241 gattcttaat ctatttatca tacaattata aaaaaggagg tgctgcttat gaaagctatc
      301 catatttctt tgcttttttt cgctagtgca ttgattttat ttgctattca tctttgttcc
      361 cagccttata tcaataacaa cggaatatta gtggaaccag cattcttttg cttaccactc
      421 ggctttttgt cccttcttgc ctccgcaggc ttcggatttg ttgctttttt taaacgatca
      481 aaacaacaaa tctgacttat aaaatttacg taaaagagat cgtgacaata atcgtgtgat
      541 attcccaaat ttcgaataaa aggtgcccct tgccatctct aactcctcag tcacaagtta
      601 caatattcat aaaccatgaa aagcttgttt attgaaattg ctccttgtga cttggagcta
      661 agtgacagat tacaacctct tttgcatcat aagactttac tccttgaaaa acattactaa
      721 cttttgatat gtttcttgcg cttgtttatt gctgaaatca aactgatatt catggttcaa
      781 aacagcattt ttctccgcat aatctacttt tgtgactttc acccccgctt tctcaaatac
      841 agaagttact tcttccattt gtttcgtaaa cgtatttgta tttccatccg ttaagaatgt
      901 gttttgcgtc caacgatccg catgtctgac caaggctaaa cttttgacct tctctgattg
      961 ttcaaagtcc ttattatcaa agtaagccca tccacatttt tcataaagaa agctagagat
     1021 tttatcactt acttgagtaa gtgcagaagg atccaacaga ccagacaaag aaataaactt
     1081 cgtaatctgc ttgttctcca aagtcggatc aagatgatca gcttttttaa tttcttcatt
     1141 ttgaagtgct aacaaatatt ctccactaat ttgtgcacct gccgaatttc caccaatcga
     1201 aatattcgac caatctgctt gatcttttat ctctttttta gctgcttgaa tggcttcgtt
     1261 caattggata actggtgtag gatgttttgc ttccggtgat cgttcataat ctatatttac
     1321 gatgatttgc tgcgtgtctg aacatagata ttccaaataa tcacgacaat catttttatc
     1381 ccctcctaca tatgcgcctc catgcatcca gaaaagaatc ggtaattttt tcttttccac
     1441 tttcggtgtg tagatatcca tattattatt tttgtacttt gaaggatagg atatttcttt
     1501 ttttatatcg atttgttcga ttttttgctg gaaatcatct ggtcgggcat atacttgttc
     1561 tttttcactt gtgtcaaatt gttttctaac aagcaagcta atcggctttg gtgtgatttg
     1621 attaatcaaa aatacagtga ctaaaataac tacgatgata actggtactg caatcactat
     1681 tcgcttgaac catttcattc atttaccctc tctttcttcg ccaaatccat gtgataatcg
     1741 tgctaacaga taaaaatcca cctgaataaa tgagaagcga atgtttcttt tctccagttt
     1801 tcggcaacat tctttttttt gcatctgtct ttttttgtac tttcactgtc atctgaatcg
     1861 gttgcgtatc atcacttgat tgatcctctc gaggttcatt ctttccctta ttgtctgcat
     1921 tctctgtcgg atcttgcttt tttggtggag ctactgggat ttttttgtaa tagaaaataa
     1981 tagttggtat cgtctcatta aaaatccctt cagtcggtcc ttcaacattt gctaattcgt
     2041 atccttcgaa tgtcttttgc tctgtcatat aattttctcc ataagctccg taaagctgga
     2101 tttcgtcctg gtctttgagt agacttttat tgttttcatc cacatacttt acgtttatct
     2161 gatcatggaa cactactttc aaaaagtccg ttacatgttt cgtgtaggca gcatggtcaa
     2221 cttcctgtgc ctttccatgc cctgctcctt ctactaataa aagatcttta taccctcctg
     2281 ataactcata taatttttgc gcgttagaca caggtactac atcatctgct gttccatgga
     2341 taatcaattt aggcaatgaa tctgacattc caatcgtagg tagagcagct tttgtatcca
     2401 aatgcatctc ctgatcaaaa tagtattgtt tcaaaaggcc cataaataaa tcttcatgct
     2461 cttgatcccc aatgatgtct aactgatttt ttatggcatc cggtagtgcg ggggctactg
     2521 tctggctgta cgtaaaacgt agttgttcat caattgatgc aaatccgcaa ttttcaataa
     2581 ttcctttgac attgacagga atatctttcg ctaatacaga catcatcgta gctgctccca
     2641 tcgaaccacc ataaagtaaa atcttttgag atggccgctc tcgaacttct tgatttatcc
     2701 agtaaaggac gtcatctgac tcatattgtc cataggtcac ataatcgcct ccgctattac
     2761 cagtcgctct ggaatcaaca aataataaat tatacccggc cttataaaaa tcattgtatt
     2821 cttctgtaac gatcccgtta ttccaattcc ctcgaaatcc gccatggatc atgactgttt
     2881 tatctgaacc ttgatcgaca taatatgcat atacatccat cattttgcca tcttgtttgt
     2941 ttagtacagt ttgatgaagt tgttttgtat ttggatagaa gcctgtgttt tctgtaggcg
     3001 ttgtcccatc atctgtacct ccaagcatcg acatcaacac actgtttttt gcaaaagcga
     3061 tatcgtacaa tagataagta gcaatcagat gtttgataat ctctttatct acaccataat
     3121 cattaacagc aagctcagtg actaataact ctattaattt tgacttgatg tcttctgttg
     3181 gtccattaag tatttctgtc atctgtttac cgaagtaatt caaatattct ttaccattga
     3241 aattttcacc tttaccgata tcatcatttt ctgtcccatt ctctttgtca cttactttag
     3301 cagaagagga ttcgtcgttt gacatttttg tagctgttgc ttcttctgtt tgttggttat
     3361 ctgtggtagc ggtcgtttct tgatctgtgc tagtaacaga tgtctcaacc gttgtttcag
     3421 tatttgtttc tgtttgtgaa gtactcgatt tcgtttcatt tccagcatca gtagtttctg
     3481 tggtttctac ctcccgagat tccgattcgg aagaaatgct ctctccatat actgtccatt
     3541 gcgggacgct tgtacttaca gcaaataaga tgaccaacgc tccctttaat acttgttttg
     3601 ttctcaagct attcactcct ttttatgttt ctaggagaat tatatgtaaa cgcaaacatt
     3661 ttttatacaa aaaataaatg gtttatccca taaaaataga acaggattat tttctcattt
     3721 tacgtaataa agcagaatgg ataaaccagt atccattctg ctttttttaa acgaagaaac
     3781 aacttattgc tccaaaaatg tcagaaatcg tttatcccaa attttctcgt aaaaagcgat
     3841 tgtttccttt gctttcataa aaggattatc ataagttgtc gttgctcctc tacacatcat
     3901 aagctggtat cctaatccat gagcaaactt cacagtcgta tctatacaat actctgcttg
     3961 tgccccacat aattcgatcg aatgacagtc caattcagtt aataaaacac tcaaattagt
     4021 cttataaaat gaattcgcat gggtttttct cacaaaataa ctgtgctcag gaatagataa
     4081 ttccgacaat agctcccatg gttttgtttg tggcactaat tgagcatctt cgtgttgaac
     4141 gaaaataact ggtttcccaa tcgtttcgta tgttttgatt cttttattca ctaatgctgt
     4201 cactgtttct aattcgtgta tcggcgaaga atcaaaacat accccttttt gtaaatcgat
     4261 cactactatt gcgtcggctg tgtttgacat acttgtgcct tctttctttt ttcttgattt
     4321 aatagtattt cttatctagc tagttccatt atacgtgaca acattttctt attttacacc
     4381 tctaaatggc acttttcgac tatttcttca tgttattatt tcataaatac ttagctattg
     4441 gaggaatgta tgatgattaa attgtataca ggttctggct gctcgtcttc aagacgcgca
     4501 aaacgttggc ttatcgtcca tgatataccg tttatcgaac agaacttaat tcgtcaacca
     4561 ctaaccaaaa atgaatttat ccacttacta aaattgacaa acaacggcgt gaccgaaatg
     4621 ctctctactc attctattag ttataaaaat cttgttcaca gaatagatga actcagtttg
     4681 agtgaactat atattgagat tcgtacatcc ccttctttat tgaaaaaacc gattatctac
     4741 gaccaatctg caaaactttt gactggattt agcgaagata aaatcaggat gttcctttct
     4801 aaaactaaaa gaaagcaaga gctcaacgaa ctcttgcttt ctggtcatct tgtcgatttt
     4861 aaagaaaata atctttttgc aatgtcttaa ttttggcata aacaatctgc ttttcttaat
     4921 taacgaaatc caatttttcc aatatggatc tatttttctc attcctgatt tttatgattt
     4981 cagctttcag acgcgcccaa tcattttttg taggttgtcc attccagtaa aatatctgtg
     5041 tattcgtttg atcatatgcc tttttgtcga taggataatc gctaatgaca atatcaggta
     5101 actgatcagc ggatgcacta tattttattg ggcgttcagt catagataat atccttttct
     5161 ttgcccataa ttcctgtgtt ttaccatact ttgattgaat atagaatatg acaggttgtt
     5221 cgtgttttat aaaaatatcc ctgactaaca tacagtattg aaaaatcaac cgtctgttct
     5281 tttcataata agtattaagc tctggaattc tgcgagctac cccactaaaa aactgttgca
     5341 tgattggata catcactgga aatatatgct gaaaatctct gtcttctgct gattttccaa
     5401 aagcatccac cttctttttt gtcccaaaaa cccttgaata caaatggatc attgccaaat
     5461 tgacatatag ataatttttt tctgaagttg taaaacaaat cttgaagttt ttttcaaaat
     5521 tttctacaat agacgattca atccagcccc aactttcttg tactttatct ggagtatcca
     5581 acaaatcttc ttccaaatag gtgttcatta caccaaacat aaaataaagg taagcttgct
     5641 ctgttggagt gatgtgattc atcgaccaga agtttgcttc aaaaaaaggc tctagtatgt
     5701 aatgcgtaaa ctcttctttt gttatcaatg gatgcacaaa tttgttcatc tcttctggga
     5761 catacgtaat cgagtggcct tgtttcattc gttgcaacga aattcttagt aggctgatcc
     5821 atttctttct atctatacta cgcgtatatg gcgcatgttt cgcaaatttt tctaatagtt
     5881 gacgtttatt ttttatactt gataatatat cagaatgatt ttcgtcgaat gactgccaat
     5941 acatcaagtg aaaaaagtat cggatacggt attcttctcc ttctatcgga ttaccttttt
     6001 tcaagctgca agataaatga tagtttttca aaatagtatt aagttctctt aattttcttt
     6061 gaatcgtacg caaacttata taattttttg actaagattt tctaaggatt gataagtacc
     6121 tttgtaaagt gaatccagta aaatataacc aatcgttccc tctaaaagta tagaataaac
     6181 tttactcagt aaaaactgag aagaaaactc gacttttatc atcttgtttt ctgaaagaaa
     6241 agataattta tattctggat acctttcgca aaacgattct gcttgttgaa tcaccgaatt
     6301 catcttagcc agggtgtagt ttagatctaa agcgatatcc ttcaccacat ataatttctc
     6361 gtctctttca taacaatagt tcaaaaattg atatacttgc ctttcattga tatcaataaa
     6421 ttcttcaaac aaagtacata ctccttttta tctattattt agacttattc cgacagctac
     6481 atgaataacg tacaaataga ttcgttactg atttttcaga gatactacca tgcgcagtgt
     6541 gcattgtcaa ttattcttgc tcttattttc catttgtgtt cctgttattt cctgtttctc
     6601 aaatatgtta gtaaacaaaa atagatctgg caaagctaca acttcgccag gcaatacagc
     6661 actctcgaat gaaatattca ctcaaaaatc tatcgtttta ttcaatttta gatctgttgc
     6721 ttttattctt tatttcactc gatggaagag agaacgaaaa tagcttgctc ctacaatcaa
     6781 aatgagtaac acacccatcc atgtaattag cttgttcaag gttctactac cagtttctgg
     6841 taaatcgctt tttctattat ctgtttcttt cgctttttct tgatgatcaa ttccacgaga
     6901 aacaaaatca ggctgtttta gcacactttt taggttgctc cctgcatttc tcttttgttc
     6961 cttagttggt atgataggta ccaaatcaag ttcattattt acttttcctg tgctgctttc
     7021 gctgctcgtg cttcctgtgc tactctcact actcatgctt cctgtgctac tttcactact
     7081 cgtactctcc gtactgcttt cgctgctcgt gcttcctgtg ctactttcac tactcgtact
     7141 ctccgtactg ctttcgctgc tcgtgttttc tgtgctaatt tcactgctcg tactctctgt
     7201 actgctttca ccgctcgtgt tttctgtact gctttcacta cttgtgctat ccgtactgct
     7261 tggcggtcca acaactgtcc aactgcttgg tttcaaattc tccgtgatcg tcatctgtcc
     7321 atcataaaaa acaatcaaat caactttatc tttactcttg atctcatcaa tcgatagttc
     7381 tgcagaaatc attattgtat actcaatacc agcggtccat tgtacgtctt ttaaataaac
     7441 ttccccaata actttttctg aacttccatt accatcccaa ccatatgtta gaacagcatg
     7501 tacatcacta gcattcacgc ttttgtttgg tttgactgga atagtaatga cttgttgtgt
     7561 catgtcataa acaatccgat cagttttcac agtaaatgaa agattctcat ttgcccgttc
     7621 atttgcagaa gtactgattc caatcattgc tgcaattata ctcagcccaa gaataacaaa
     7681 taatacggtc gctgttttct tcatttctat cactctccat ataattacct tctatttgat
     7741 aaaggaatta tataaaatga aaatgatgag aacacgacag tcagttgtga aaatctaaca
     7801 aaagatgtcg tgtattcgct tgaatttgtt tactttttcg tttaatcaat gacagactta
     7861 aaaaagcaag accgatatca gccttgcttt cttcatcttt tattgataaa cagcagtgta
     7921 tttcttcatc cgctttgttt tatatgcata gagtaacaaa ccaacaatca agactaacaa
     7981 gaataatgta gttgatatag atgcttcagc cccaaagttt cccccagtaa ttattgtatt
     8041 ttgcgattgg atcagagtgg agaaaaaacc ttgttcttgc gttccgctga ctggtatacc
     8101 caaaatagct ccttgtgtcc aattccaagt aatatgagca ccaatagccg taaataaact
     8161 tcctgtactg tagataagtg cgctgaaaaa gcaacctgct aaaaagatat tgaaaactgc
     8221 cagaaatgtc atgttaggat tcaatccgtg caaaagagca aataatgcag cagatccaat
     8281 cattgctatt aacggaccaa atctaagcat ctgatgctga aaaaagccac ggaataaaag
     8341 ctcttctgat gcccccactg caataaataa aataaaactc gttatcagcg gcacaatgtt
     8401 tgcgtttcct gaatctaacc gatacgcccc cgtcaaatat aatggactag taataagcag
     8461 aaacactcca attccaacaa cgagacctcc cgctaaatac ccccagtctt ttcctcgaac
     8521 agaaacacca taatcttcta ttctttttcc ttcgacctta ctagcaagaa ataaagctag
     8581 taaaaaaaca gctaaatgtc cgaccgtacc ggcaattgct ccaatccatt cactatttcc
     8641 aataaacata tccgcaagtg cattattgac aagaattcct aaaacaaaac caacaaagac
     8701 tattaaaaca gatccaatca accgaataat cctgatcatc tttcatccct cctttatatt
     8761 aagtttaaca acccttttct ttcttgtctc gtgaaaacta tgtaaatgtt ctccatcata
     8821 gagagaatag atttttacga actaagaata tctacagcaa ccttacgaaa atgaaagact
     8881 gtctccctaa ttcttctgtt agactgtttt taataaggaa aggatgggaa attttttgaa
     8941 aaaacaacta ctaattatgt gcctttcagc atgtgcaatt ttactattct caggttgtag
     9001 caagtccaat gaaacgattg caacgtttaa aggtggaaca cttacagttc aggattttta
     9061 tgataaccca aaagttaaat caaataatca aacactatta aaaaaaatga ttgtcttcaa
     9121 agcatttgat gatgtatacg gaaaagagat ttcttcaaag gaagtaacaa aagaatataa
     9181 tgaacaaatc aaaaaactcg gtccaaacta caaggaacaa ttaaaagcag ttggccaaac
     9241 agaagagaca tataagaaac tctttaaaca gatgctggct ttccaatacg gtttgaaagc
     9301 caatgtaaaa ttaacagata aagatctaga cactgcttgg aaagaattct atccagaagt
     9361 tagtacacag atcattctat tttcgactaa agaagaagca gataaagcaa aaaaagaagc
     9421 aaatgaagga gaaaatttct ccaagcttgt tcaagcttat ggaaaaaata aaacattaaa
     9481 ggaaactgat ggaaaaatga attttgattc aaccaatcca gagattccta ctgaagtaaa
     9541 aaaagcagca tttaaactaa aaaacgggga agtttctgat atcattcctg taacagatcc
     9601 aacgacttat cagcaaagct attatctagt aaaaatggta aaaaaacagg ataaaggttc
     9661 taataaagac aaatacaaat ctgagctaga aaaaattgct accgaagcaa aaactcttga
     9721 tacggaattc atggataaaa caatccgtaa agtgatgaaa aaagacaatg taactattaa
     9781 agatccttat gtaaaaaata tattcaaata aacaaatttg acccaattgg ggatgtggca
     9841 aaatttgaga agtgattttt ggaaattcct tcttattttt gaggcaaacc acttttgcca
     9901 caacttcatc ggggcctttt tttatttgta ccaaaacaaa tcactggatt tgttacaatc
     9961 atgaggatat aatttaaaaa agttttttat ttctatcaca tgaatcaatg cgccattaag
    10021 tagattcttt catgcttccc tattttttca cttgccgtat cgcttcaaaa acaactatca
    10081 taaactcaaa ctcgactgaa aagttatttt aaaaaagact aaaaatatcc cttctggact
    10141 tgttcttctt attctgttat actagagaaa cacgaaagat atttttatgc gacacttttc
    10201 atttgttttt atgaaattta ttcaaaactg gcgtaacttc agagttacaa ggatgtatta
    10261 gaggttaggg aatacatcgt ttaaatgaga aataagtaat agttagtaat agtcacccaa
    10321 actttaaatt aatgaatatt gaatcgtcag atagcctcct cgagttttcg agaaggctat
    10381 cgtttatttt atggagaaat tgattcagaa gtaatcagcg acctttttta gttcaccaat
    10441 cgtttctgct tggaaagttt gcacccaatc ttcttggaag cccctcactg ctttatcaat
    10501 cgagggcatc tctaatactt gatctagtag ctctttacct aacgttatcg ctgagctcca
    10561 gatcgaatgg ctgtagtgac ttgttcacaa tttttgaaac tagctgctaa tatttttgtt
    10621 ggtctttgcg tacgttcgat ctcagtccgc aacgaatcaa tcactttcat agcatcaatg
    10681 ccttgatttt gcatcctgtt ataataaggt gcaatataat ctgctccatt attaatagct
    10741 aaataccctt gtaattctgt gtaaatagct gtaccggtaa tcaaataacc ttcttttttc
    10801 aactttttga tcgcttgaag gcctgcttga ttcaccggca ccttgataaa cgtatctttt
    10861 cccagttctt ctatgataac tcgagcatcc tctatcattt cttcttccgt tcttcctatt
    10921 gtctgaatat ggataggaat ctgtccgatt atttttttga tttctttcat atgcgagaag
    10981 aagtttgacg ctccttcttt tttcagaatt gtaggatttg ttgttacgcc tgttaaattc
    11041 aaaaactgtt gacaagaata aatacttttt gtatttgctg tatcaagcat gatttccata
    11101 tagctttccc ccattctgat cctcttgatc gattttaatg attgtcccct ttcttaaatg
    11161 cggcatttcc ttatcttcta catacattgt tcctggaagt tccggctcat gagatccatc
    11221 aaattttatc gtgatatgtc ctaattcatt caaattagtt tggacaacat ctcctactgc
    11281 tgtgattaca tacatttgtt tatcaaaaca gagcttcata tcacaagtta tttcttttct
    11341 tacctgattc aattcaatct tataacaata atctgttaat gtttccggtg cttcatctcc
    11401 aaataaaatc attatttttt ctttcctgaa tacttctgct tctataccaa catctgttac
    11461 aaccgtttca aagatcgtca tctctgttcc tcctcgtaaa atatcatttg tctaatagtt
    11521 tttattcaaa atgtttctag attataaaat ttattgaata acacattcct aacggaattt
    11581 gtttcgatat tgtgtgattt ctcagagtac aatggatgta tgga
//
LOCUS       DAYYAO010000086        11592 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000086 DAYYAO010000000
VERSION     DAYYAO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.089"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(289..774)
                     /locus_tag="ACHARI_002261"
     CDS             complement(289..774)
                     /locus_tag="ACHARI_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8443275.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    864..1209
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1254..1895
                     /locus_tag="ACHARI_002262"
     CDS             1254..1895
                     /locus_tag="ACHARI_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8443276.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1914..2549
                     /locus_tag="ACHARI_002263"
     CDS             1914..2549
                     /locus_tag="ACHARI_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8443277.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2567..3382
                     /locus_tag="ACHARI_002264"
     CDS             2567..3382
                     /locus_tag="ACHARI_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8443278.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3701..5137
                     /locus_tag="ACHARI_002265"
     CDS             3701..5137
                     /locus_tag="ACHARI_002265"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8443279.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5326..7773
                     /locus_tag="ACHARI_002266"
     CDS             5326..7773
                     /locus_tag="ACHARI_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8443280.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            8003..9070
                     /gene="rlmN"
                     /locus_tag="ACHARI_002267"
     CDS             8003..9070
                     /gene="rlmN"
                     /locus_tag="ACHARI_002267"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8443281.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9060..9266
                     /locus_tag="ACHARI_002268"
     CDS             9060..9266
                     /locus_tag="ACHARI_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443282.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9263..10366)
                     /locus_tag="ACHARI_002269"
     CDS             complement(9263..10366)
                     /locus_tag="ACHARI_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8443283.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10918..11466
                     /gene="aac(6')"
                     /locus_tag="ACHARI_002270"
     CDS             10918..11466
                     /gene="aac(6')"
                     /locus_tag="ACHARI_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8443284.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3860 a         1950 c         2492 g         3290 t
ORIGIN      
        1 aatataaaaa agtttagtta gaatataata ttttttgtta aatatgactg aaatcaaaag
       61 aataaaattc cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt
      121 agaatcaata aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt
      181 tcatactagt tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa
      241 ataaagaaaa agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc
      301 ataaactggc agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat
      361 caacgtagtg gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc
      421 atcgatcacg acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt
      481 acctgctgga acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt
      541 ttcttttaat tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac
      601 aagatagatt tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg
      661 tagggaaaaa gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga
      721 gatacccagt ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca
      781 tcaccctcct ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat
      841 gtaaattaca cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt
      901 tttagagagt ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag
      961 tcgattttct gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg
     1021 ctagatgacc agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac
     1081 gtgattaacg tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac
     1141 gactgtgtga ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac
     1201 gtcctcttgg catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt
     1261 atttgattga agttatgccg caattattag taggagcact cacaacattg aaattatttt
     1321 tatttacctt gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc
     1381 attttaagcc gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc
     1441 tgttgttgca actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc
     1501 gtttcgaagc agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct
     1561 ttcgtggagg gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat
     1621 taagtccctt tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac
     1681 cttctgtagg gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag
     1741 gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc
     1801 ctttgctagg cgtggcaatc atctatctta cattgactgg tattctaaca atcgtaatga
     1861 aacaagttga aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg
     1921 aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa
     1981 tcattccaga tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc
     2041 ttttacgtat cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg
     2101 taccgttcaa cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag
     2161 acttccagct atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg
     2221 tattgaaaca gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag
     2281 ggattagtga tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg
     2341 tagcgattgc cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa
     2401 gtgctttaga tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag
     2461 atggggtaac ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc
     2521 aattactaaa agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag
     2581 ttttctattc gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac
     2641 ttcagcagat aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga
     2701 tacatttgtt cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga
     2761 tttagccaaa gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg
     2821 gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc
     2881 caaaacgccg gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca
     2941 agtgctgatc acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag
     3001 attaggtgcg caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt
     3061 aaaagagata gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat
     3121 cgatctgaaa agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta
     3181 tttgaaacaa agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga
     3241 ggattttgca gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc
     3301 cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga
     3361 agatgttacg ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc
     3421 tttttccgaa taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg
     3481 ctcacaacct cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta
     3541 tgcaaaaatg tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact
     3601 ttttcacaac ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta
     3661 aaagaggaac gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac
     3721 ctcatttcca aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc
     3781 ttggcaagaa gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa
     3841 gacatttaaa ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga
     3901 tattgcttta atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg
     3961 gatttttcct aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt
     4021 gatcgacgaa ttgattgaaa acgatattga acctatgttg actttatatc attgggatct
     4081 tccacaagca ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt
     4141 cactcattat gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag
     4201 tttgaatgaa caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg
     4261 tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc
     4321 tgtcatcaat aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta
     4381 tacgccaaat tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga
     4441 agacctgatg gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc
     4501 catgaggttc ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt
     4561 gctagaatct gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc
     4621 attcaatcca ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc
     4681 atcagaagaa actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg
     4741 aacaaattgg gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac
     4801 aagccgttac cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt
     4861 gacagaagat aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa
     4921 agccatcaaa gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta
     4981 tacagatttg cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga
     5041 tcaagacgaa acacaagaag gatctttgga acgatataag aaagacagtt attactggta
     5101 ccaaaaactg attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg
     5161 tacggctgga tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg
     5221 taaaataagc tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct
     5281 tacttttttt tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa
     5341 cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa
     5401 caaatgctga cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta
     5461 aaattaggtt ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact
     5521 taccaaatcc tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca
     5581 gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc
     5641 ttattattgt tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg
     5701 gggacgacat gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt
     5761 ggtggtggga tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct
     5821 ggaagttatt tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg
     5881 gaaggctatc agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg
     5941 gaaggtgatc ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag
     6001 cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct
     6061 gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg
     6121 caggaaccag aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa
     6181 gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat
     6241 acagcagaag gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag
     6301 attgaagcag aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct
     6361 ataccaactg tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta
     6421 gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca
     6481 tccgtcacaa aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac
     6541 ttaacagatg atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt
     6601 ccaggtaaat cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt
     6661 agagaaatca tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga
     6721 cgtgatgttt ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg
     6781 gctggttcga ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta
     6841 atgcgtgcta agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg
     6901 aatatgtaca acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca
     6961 gcgcaagcct tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca
     7021 accggagtaa gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac
     7081 ggaacgaaac atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg
     7141 atgatggtag ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg
     7201 gcagcgggga ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga
     7261 atcatcaaag caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct
     7321 cggacgatca ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc
     7381 ttgccaatgg gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa
     7441 gtagaacggg tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg
     7501 atgccgacag atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt
     7561 gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga
     7621 agatttcgga tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga
     7681 gttgttggcc cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca
     7741 tcagaaacgt tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac
     7801 gaataaccaa taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat
     7861 cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc
     7921 gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt
     7981 tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga
     8041 attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga
     8101 atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt
     8161 gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga
     8221 agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac
     8281 tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa
     8341 tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg
     8401 agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga
     8461 acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt
     8521 aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat
     8581 tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca
     8641 agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg
     8701 gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa
     8761 aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga
     8821 gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt
     8881 gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt
     8941 attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca
     9001 tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt
     9061 tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa
     9121 ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt
     9181 ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct
     9241 tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg
     9301 tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat
     9361 aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg
     9421 tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca
     9481 catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat
     9541 aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat
     9601 agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg
     9661 tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac
     9721 tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt
     9781 ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga
     9841 cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa
     9901 taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca
     9961 ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa
    10021 tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc
    10081 aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga
    10141 tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct
    10201 ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc
    10261 ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg
    10321 catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc atttccttct
    10381 ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg
    10441 gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa
    10501 tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa
    10561 tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat
    10621 tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt
    10681 ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga
    10741 tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt ttattttata
    10801 agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag
    10861 aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg
    10921 ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg
    10981 agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg
    11041 aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt
    11101 gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc
    11161 cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc
    11221 agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt
    11281 caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa
    11341 catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca
    11401 aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct
    11461 caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac
    11521 gaaaattact tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg
    11581 aacaccgtgt ac
//
LOCUS       DAYYAO010000087        11232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000087 DAYYAO010000000
VERSION     DAYYAO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.090"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(259..1107)
                     /locus_tag="ACHARI_002271"
     CDS             complement(259..1107)
                     /locus_tag="ACHARI_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8443285.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1271..2173
                     /gene="gnd"
                     /locus_tag="ACHARI_002272"
     CDS             1271..2173
                     /gene="gnd"
                     /locus_tag="ACHARI_002272"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8443286.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2175..3176
                     /locus_tag="ACHARI_002273"
     CDS             2175..3176
                     /locus_tag="ACHARI_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8443287.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3173..3457
                     /locus_tag="ACHARI_002274"
     CDS             3173..3457
                     /locus_tag="ACHARI_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8443288.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3490..4287
                     /locus_tag="ACHARI_002275"
     CDS             3490..4287
                     /locus_tag="ACHARI_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8443289.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4301..5134
                     /locus_tag="ACHARI_002276"
     CDS             4301..5134
                     /locus_tag="ACHARI_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8443290.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5152..6009
                     /locus_tag="ACHARI_002277"
     CDS             5152..6009
                     /locus_tag="ACHARI_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8443291.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6031..6540
                     /locus_tag="ACHARI_002278"
     CDS             6031..6540
                     /locus_tag="ACHARI_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8443292.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6577..6978
                     /locus_tag="ACHARI_002279"
     CDS             6577..6978
                     /locus_tag="ACHARI_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443293.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7090..7443
                     /locus_tag="ACHARI_002280"
     CDS             7090..7443
                     /locus_tag="ACHARI_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443294.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7477..7920
                     /locus_tag="ACHARI_002281"
     CDS             7477..7920
                     /locus_tag="ACHARI_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8443295.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7937..9022
                     /gene="uxuA"
                     /locus_tag="ACHARI_002282"
     CDS             7937..9022
                     /gene="uxuA"
                     /locus_tag="ACHARI_002282"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8443296.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9015..9728
                     /locus_tag="ACHARI_002283"
     CDS             9015..9728
                     /locus_tag="ACHARI_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8443297.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9935..10084)
                     /locus_tag="ACHARI_002284"
     CDS             complement(9935..10084)
                     /locus_tag="ACHARI_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443298.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10480..10680
                     /locus_tag="ACHARI_002285"
     CDS             10480..10680
                     /locus_tag="ACHARI_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8443299.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3773 a         1753 c         2416 g         3290 t
ORIGIN      
        1 ttttagaaaa taagaaacaa aaataataaa aaaaagaaaa gataaatttt ttactactgt
       61 atactaactt tgttatcgca tctaacaagt aactcgaata ataccaatac aaccttctcc
      121 aaagatgcga tgacatacgt tctaacccac cgactattga tgttctgtac cacccttgca
      181 gaaccaaaaa agccactacc aaatatttgg tagtggcttt ttttgtttta tggatttttc
      241 gctttttctg ttatggtgct attctttgat ttcagaaata gtatgtcgaa ctttagcgat
      301 agaacgacgt gattcttttt cgttgtaaaa catcaaaatc acatatagag agtccaaaat
      361 gcttagttga gaaatacggg aagctaaagc ttcagatcgg aattctgttt cttcagaaat
      421 agaaataaaa acgacatcgc caagtttagc aagggaagaa ttagcttggc tagtaataac
      481 gattgttttt gctccagttt ttcttacggt ttcagcaata tggattgttt cttttgattg
      541 ccctgtgtga gaaatcaaaa cagcacagtc tttttccgtc aataaagaag cttccatcaa
      601 ttgaatatgg taatccgtgc tgtgagaagt tgcgataggg gaacgcaaaa atttatgata
      661 agcgtcagtt gctacaatct ctgaaccgcc aacaccgaaa aaatagacac gtttagattc
      721 attgataata gtagccgctt tttttagatc cttttgttgt aacaactttt ttgtgtcatt
      781 taaagttgaa atactagact cgaacacctt ttgcgccata gtgagctcag tatctgtttt
      841 ctgaatattt tcgtggatag aaatagcaga tagatcattt tcttggatca acatggccat
      901 tttaaaatct ttaaatccat tataaccaag tttttttgta aattgaaaaa aagtagagtc
      961 agcaatgccc agttcacttg ctaaattgct aataggacta tgacttactt tgcttggatt
     1021 ttctaaaata taatcggcga tagcctgttc ttttgggctg aagtcgttgt acattgtttt
     1081 gatttgtagt cttatggaga cgctcatgag aattcctcat tttcataata agattatcat
     1141 tttttccgtt ctcattaatg ttaaagatac aaggagagta ggaaaaaatc aaacaaaaaa
     1201 tatttttgta aaatctattt acaaaaaaat ttttttgtat aatgagggta taagaaatgg
     1261 aggcgctatc atggaaattg gtgtaattgg tttgggaaaa atgggattga atttagcttt
     1321 aaacttaaaa gagcaccatc acaaagtaca aggactcgac atttcaagta cagctgttag
     1381 tcaggcaaga gaaaatgaca ttcctgctta tcaagagatc tctgaatttc tagctacttt
     1441 tgagcagaaa aaaatcatct ggttaatgct tcctgctgga gagataaccg aaaacatgtt
     1501 aagaaaactt tttccaaaac tcggaccagg agacattatt attgaaggcg gaaactctta
     1561 ttataaagat tcaatccgtc gagcagaaga gtttcagaaa aatgaaatcg gttacttgga
     1621 ttgtgggact tctggaggaa tcgaaggagc acgacatcaa gcctgcttaa tgattggagg
     1681 agataaagaa acttttctat ccgtggaaca attattcaaa gatgtagcaa ttgaaagagg
     1741 atatctgtat gctggtgcat ccggaagcgg acacttcttg aaaatgatcc ataatggaat
     1801 cgaatatgga atgatgcaag caatcggtga aggattccaa cttgtacaaa aaagtgagta
     1861 tgcttttgac ttggaacaag tcgcacgtgt ttggaatcat ggttcggtga ttcgcggctg
     1921 gctgatggaa atcatggaag atcagttaaa cgaacatccc aatttaagta attatcgggg
     1981 aatcgtttct gcatcaggtg aagcaaaatg gacggttgaa acagcgctag aaatggctgt
     2041 tccagttcca gtaattgcaa ctagtttgtt catgaggaat ctttcacagg aagaagatag
     2101 tttttcagca aaagtagttt cagcactaag aaatgggttt ggcggacatg aaatagttga
     2161 aaagggtgag taaaatgcca gattatcata tagcaatcgt taattccagc agtttcggaa
     2221 gagtctttcc acaccacttg gaaagattaa agtcagttgg agacgttcat ttctttactg
     2281 ttgatcaaga gattccagga aaagaactag cggagttact taaaggattc aatatcatta
     2341 ttgcaagtgt tacgccattt tttcgacgag atttttttga aaacaaagat gaactgctat
     2401 tgatttcccg acatggtatt ggttacaaca atattgattt aaaagcagca aaagaacacc
     2461 agacaatcgt atctatcata cctgctttaa ttgaacgaaa tgctgtagca gaaaataata
     2521 taactaattt gttgtccatg cttcgacgca cgacacaagc acaagcaaga gtcaaggacg
     2581 atcgatggga agatcgggcg gagttcgtcg gtaggacgct gtttaacaaa acagttggtg
     2641 tgattggtgc aggaaatacc ggaagcggag taatcgaaat tttgcgtaac ggttttcgct
     2701 gtgatgttct agcttatgat ccttacaaat cagatttata catcgaaagc tttggtgcaa
     2761 aaaaagtaga acttcaagaa ttactggaac aatcagatat catttgttta tgtgcgaatt
     2821 tgacagaaga gaactatcac atgatttctg ataaagaagt cgatcagatg aaagaaaatg
     2881 tttatctatc taatagtgca agaggcgctc tagttgatga agaagcgatg attaatggtc
     2941 tgaaaagcgg aaagatcgca ggatatgcca cagatgtttt agaagaagaa ccgggacgtc
     3001 aagatcatcc gtatttagcg tttgaacaag tagtgattac gccacatata tcggcctaca
     3061 caagggaatg tttagaagca atgggtgaaa aatgtgtcag agatgtcgaa cagatcagtc
     3121 agcaccttct tcctgaacgt tctgtccaga caaagagcaa ctatataaaa ggatgatgaa
     3181 gaaaatggtg aacggtttaa aagtgaaaac aggccctcaa ttttatttat atgaagaagg
     3241 tggtatttca aaagtttctg atctcttgaa atcatacggc gcgaaaagag tactggtcac
     3301 ccacggaacg gtatcatggg aaaaggcgct acctaaatta gtatttttga atgacgaaac
     3361 gattcaattt ttttatcatc gttattctgg agaatgtagt tatgctgaag ccagacgaat
     3421 agctacaatc atcaaaaaaa tgaaatcgat ttcgtagttg gcgttggcgg aggaaaactg
     3481 accgatctag tgggttatag tgccaatctt gcaagaatcg atttcggagt catccctact
     3541 ttagctagta attgtgctcc atggacacct cttgctgtca tgtataagga gaatggacaa
     3601 gcagaaggga aaacagaaca cttttacaga caagctgctt tttttataac agatccccaa
     3661 ttagtgattg attcccctgt tccatatttc attgccgggt tagcagatac actagcaaaa
     3721 tggtatgaat ctgagctcat cttagaacaa gattttttgc aagatgaatc atttttgaag
     3781 ctagcacaag atactgcgaa gatctgcaaa gaagagattt tgaatcattc tgctaaagca
     3841 atccaagata tggcacaaag aaaactgaca tcggagttca aacagctttc agagattatc
     3901 tttgctgttg ctggtttagt tggaggatta ggagataaat acgcaagaaa tgcggcagca
     3961 catgctatgc atgacgctat cagtaaatat attccggaaa gccatcgttt tctacatgga
     4021 gaaaaagtag cgtatgggat tttctatcaa ctggcactag aaggtcgctg ggaaaaaata
     4081 gacgagttat atcctttgta tcgggaatta gaactgccgg tttctctaga acaaatggga
     4141 ttatttccga aagataaaca gataatagat aaaatggctg aatttattga ttcgaaagag
     4201 aaagtgcatt tgattcctat agaggtaaca cagaaatcac tgatacagtc aatgatggaa
     4261 ctggaaggtt atatcagaaa gatctgaagg aggaggacac atggaaacat taagtgtagt
     4321 acaaagtttg ttgattgctt tatgggtagc cgcggttatg tcccgctggt taggtggagg
     4381 agccacgttg accttacgtt tttctccttt gatgactgga ttagtcgtcg ggttgatcat
     4441 gggggaagtt gcacaagcga tgattgttac agccgctttg caaatgattt atatgggggt
     4501 tttttctcca ggaggatcaa tgccagctga accatctatt gcagcagcta tcgcagtacc
     4561 agtcgcatta ttaggggatc tgtcgccaga agcagcgatt gccgttgcgg tgccagtcgg
     4621 attgctcgga agttacttgt atcagtttcg ttttttcatc aatacttttt taggtaaata
     4681 tacagacaaa gctgtagaag atttgaatac aaaagctatg gcccgatcaa ttattcttta
     4741 tccaacggtt gcctcatttt tattattcgt tccgcttgtt ttcattgctt tatatttagg
     4801 ggcgccattg atcgctgatg taatcaaagc tttagaggga acagtcgtta tccacattct
     4861 tgaagtagta ggtggagggt tagcggcaat cggtatcgcg acaactgtat acgttatcgg
     4921 tcgaaaagat tatctggtct ttttcttcct tgcttatttt atgagtgttg ttttgaaatc
     4981 tttagatatt acgatggtta cttatgccat ttttgggatt attatcgctt tgatctttgt
     5041 ccagtcgcaa aaagggaaac aagtacagcc tgccacaagc aataacaatt cagcatcttt
     5101 tgatgacgaa gatgactatg atgacggatt ttaatttagg aggaatggga aatggctgaa
     5161 ataatggata atcaaacagg tgctcctgaa gaaatcacga aaaaagatgt aacaaaagcc
     5221 tatctgagat ggcattttgc aaacgaaatt cctcactctt ttgaacgtta cttggcgcca
     5281 tcactactat acggaatgat gccaatcttg aaaaaacttt ataaagatga agactcttta
     5341 aaagcagcat ataaacggca gttactgttc ttcaacactc aattaagctg gggtggcggt
     5401 gtgattactg gcctaatgtc ttctatggaa cagcaaagag cagaagaaga gtatgaagaa
     5461 aaagagatct tgatgcagga tgacttgatg tataacacga aagcgggatt gatgggggca
     5521 ctagcaggaa tcggagatgc aatcgattca ggaaccgtcc aatatatttt tatcgcaatc
     5581 gcggtacctt gggcacaaca gggaagtcct ttaggcgcac tttttccttt tatctgcttt
     5641 gggttgtatc aggttttact aggggtattt ttcgcaagac aagctttcac tatgggccgt
     5701 aatgctacag gcctaatgca aaacactggt gttcagacag cgattgaaat gctatcagtt
     5761 ttagggttgt ttatgatggg ggtgcttgcg ggaaactatg taaaagtaga atcttcattg
     5821 aaatttgcga tatccggacg tgagtttgtt atccaggaaa ttcttgatca aatcgtgcca
     5881 gggatattac ctttagctgt cgtaatgggt gtgtacttgt tctatacgaa aaaaggattg
     5941 aaagtcacac aagcattatt atggctgact ggaatattaa ttgtgctagc aggaatcggt
     6001 attttataaa aagaagggaa cgtgaataag atgggtgtag tcaatttagc gcgtgtggat
     6061 gagcgtttga tccatgggca agtaatggta acgttatctc aaaaaagtgg agtgaattcc
     6121 atttttgttg tagacgaagt tgtagcaaaa gataaattta tgagagatct ttacaaaaat
     6181 gcaggaagcc gtacaggaca aaaaacaatt gtcattacac cagagaaagc taaattctat
     6241 tgggatgaat atcaatttaa agaatacaat tgtattttga tcgcaaaaac agtccaaacg
     6301 atttacgact tagtaaaaca cggaatcccc atggaagaat tgaatattgg agggattgct
     6361 caaaaaaatc ctgatacaga tcttttagtg acgaaatctg tttatttaaa taaatcagat
     6421 gctgaaaaat tgaaagaaat gaatgaacaa tatggcgtaa agaatattta ttttcaagcc
     6481 actccatctg cacctaaaac aggattaaaa gatgtattga acaagtttga tttgtcataa
     6541 acaaatgctt agaaaaaaga taaagagggc gaaaaaatga agaagaacga gctgtttcgt
     6601 gattgggaat ttcgttatcg ctatatttac cgtaagcgcc gtacaaaaaa gagtaaacag
     6661 cgttttcttt cagcattagt atcagatatt tacagtatga gaacagatgt gacagtcatt
     6721 gcttacgata cgctagccta ccgatcaaaa aatatttatg ttggagatat cgaaaaagca
     6781 gagaaagtta tctgtactta ttacgataca cccgttcatg cattaggaag ttatttcatg
     6841 tttgattgga aagaccaaag gaaaaaaaca atctactcta tattattgtc cttcattttg
     6901 ttgttcagtt taggttggtg gggaatgatg atatataaca aaaatcctca tcacgtattc
     6961 gatctgctat ccgtataaac aggtatcact gtactagcat ttgggagcta ctttttctta
     7021 ttgggaaaag cagctcgcgg atggcctagc agacaaactt ttattcgaaa tacttcatcc
     7081 atattaacga tgctagaaat gatccgtacg attgatgatc cgagtgtagc gtatgcgttt
     7141 gttgatgaag gctgttatgg agaaaagggc ttagacagcg tgagatcggg tatgaaaaaa
     7201 gaggctattc ttttttatct agactctgtt ggggcagata caccgctaca gttttcggga
     7261 aattattttt caaataaaga acagtggctg aaacaagttg ataagttgaa agaaaaaaac
     7321 gtcaattata tttttagtgc aagaaaaaag caagcgcagt tcttctattt gaccaaaaca
     7381 gatttacgag ggaaaacatt taattggcaa aatgccaatc agattattgc tctttttcga
     7441 taagtaaaaa gtgcaaaaca aggagtgaga agactaatgt tggaaatcat gatcgttaca
     7501 catggtaagt tgagcgaagg actgaaagat gcggcggaag taattgtcgg gacatcgagc
     7561 catattgaaa caatgtgctt agagtctggg gaggacatcc aaacattcgg agagaaggtc
     7621 aaagaaaaat taactatgat caacacagca gaacccatgc tagtatttgt tgatctaatt
     7681 agcgcaagtc cgtacaatca agcgctcttg gcagtcaatg aactgactga agaaaagaaa
     7741 gcagccactt atgtcatagg gggcgtgaat ctgccaatgt tgttagaagc aatcaatcat
     7801 cagatgcttc acacaccgat agaagaagcg gtggaacaaa ttgtcaaaca aggaaaagat
     7861 aatttaactg tttggcatgc ttcagcggaa gaaaaaaaat tagatgagga cgatttttag
     7921 aaggaaggct gaaaagatga agtggggatt tagatggtat ggtgaaaaag gggattcgat
     7981 cccattaaaa tatgttcgcc aaataccggg aataaatggg ataattggaa ctttgctgac
     8041 taaaatgcct ggagatgttt gggagatttc agaaattgag gagttgaaag catctgttga
     8101 gagccaagga ctggaactgc ttggaattga aagtgtagcg atccacgatg caatcaaggc
     8161 agggacagat gaacgagatt actatattga taactatata cgaacaatca aaaacttgtc
     8221 tcgatgtggg gttcgattga tttgttatag cttcaaacca atttttggat gggcgaaaac
     8281 tgatttgttt aatgaaaaca acgatggaag ctattctctt ttatacgatc agaaaatcgt
     8341 cgatcagatg gagccaggag aaatgtatca attgatacac agccagtcaa agggattcaa
     8401 attgccgggt tgggaagaag aacggttgaa aaaattcgct cagctggaaa aactgtatga
     8461 aggaatgacc gaagaagaat tatttgaaaa tctgacttat tttttagaaa aagtgattcc
     8521 agtgtgcgaa aaagcagatg taaaaatggc tatccatcca gatgatccgc catgggaact
     8581 ttttggtttt cctagagtga cgaaaaatct tggcgacttg aagaaaattc taaatgcagt
     8641 agattctcca tataatggac taacgctgtg cacaggatct ttaggggcaa atccggaaaa
     8701 cgatttagtt gaaatcatcc atgaggtagg ttcgcgcatc cattttgtcc acttcagaaa
     8761 cgtggagttt ttaggagaaa gaaagttcaa agaaacggca catccctcta cagaaggctc
     8821 ccttgatatg tatcatatta tgaaagcgtt ggtgcaagaa gggttcgacg gtcctatacg
     8881 ccctgatcat ggtagaaccg tgtggggtga agtggctatg cctggatatg gcctgtatga
     8941 tcgtgcaatg ggactcactt acatgcaagg tctatatgag gcgataagta aggaaaaacc
     9001 actgggagag cattatgaat aaaatcaagc tcttcatctt tgatatggat ggccttttat
     9061 tagaaactgg acgtttagct tatcgtgctt atgtaaaatc agcacaaaag tatgattatg
     9121 aaatgcgtaa ggaagtctat tatttactaa ctggccaaac agaaatggcg ataagagaac
     9181 aaatgggcat tttgtacgga gaagatgttc catatatcaa atggcgagaa gcaatcaatc
     9241 aatataaaga aaaaatcgtg aaagaagata agagagtata tacgaaaaaa ggagcagaag
     9301 aaattttatc ttttgcaaaa gaaagaggaa tccatacaat cgtggcttcg tctaatacac
     9361 gggtaaaagt agaaatgtat ttaaaaatgg aaaatctata tgccttgttt gatcaaatca
     9421 tttcagggga tgatgtaaaa aaaggaaagc cggaaccaga gatctttttg aaagcttgtt
     9481 caaaaatgaa tattcctcca agtgaagcac ttgtatttga agactcgatt gcagggatcg
     9541 aagcagcaag gcgagcgggc attttgtctt ttctcatcaa agatcatttg atagggttgc
     9601 ccgatcataa tgggaagcat aagttgaaag tagatgtaag tttatatgaa tccaataaag
     9661 cagactatga attttatgac ctgcatcaag caaaacgatt tttagaaagt aaagggttgt
     9721 atctctaata aagaggacta ttttctagtc ctctttattt attgtcctgc acgactgagg
     9781 gaacagatga aaaagaagta attttttatt ttgaagtatc taccttattt gtagtgaaca
     9841 atttagaaca aacacaaaac gcatctactg ttatctacca gtagatgcgt ttaaagagaa
     9901 gaacgagaat cgtccatatg agggtttcca ctccttatgc agattttttt aattcttgca
     9961 ctgcttgttc gatcgtaaca cctgtcgcta catgattggt attttctttg tcaaaaacga
    10021 caaatagatt caaatctgta tccaaggtta ctcgatattg tatatctttg ttatagaatg
    10081 tcatagccat tctcctttct ttaaatactt gctgaaaggc aacgaacgcg ttgataacga
    10141 aattccattt ataagtatac caccaattga aaggttttca tagttaagaa attttaaagt
    10201 caaaagttgc agataaaagc aaataaacaa gaaaacaaaa agtgaatcaa tcgcggttat
    10261 tcaacttttt cttcgattcg aagacaatag aagacaatga attttatatt tcattgcatt
    10321 atccttgtgt tattagaaaa agcatgatat attaaatgag taatctattt tgaaacgtaa
    10381 tttgagcgat atatctacat agataaaact ccttttgcct atcaaatatt tattgcaacg
    10441 aaaaaacgta cattgatacg tatcaggagg aatttattta tgaataacgg tacagtaaaa
    10501 tggttcaacg cagacaaagg ttttggcttt atcactggtg aagatggaaa tgatgtattt
    10561 gcacattttt cagctatcca aggtgacggc ttcaaaactt tagaagaagg ccaagcagtg
    10621 tcttacgatg ttgaagatgg tcaacgtggc cctcaagcaa caaacattgt taaagcttaa
    10681 taaaaattga cttttaaccg tcttttcgaa agaaaagacg gtttttttgt acgaaaaaat
    10741 tcattggtcg ttgaatgggc tgaatagtag tgtataaaac aattttgtgg agcgtgaagc
    10801 ttttatggta gaggaaaata cgaataaagt gatgttgata aaaagattcc tatcttgaat
    10861 atgaatcgag atgtcttgaa aatgaatgag tttgaccaat cattatccta tcgaaattta
    10921 cagtttcgtt cgattggtct gtcaattaat gaaaaagtaa aaaagaatat gttctaggaa
    10981 gagggcggca catctaaatt tttaagtaac ggacagttca aatatcaagc attgaaagag
    11041 gctatcctac tgtttgaagg aatacgaata tataaaaacg acaaagagga aaaagtagtt
    11101 cattaaatcc cttcaagtga tgaaagaatg agatctaata aaacctgatc tttcatctgt
    11161 ttttttaata acgtaataat ttttttattt tttgtgcttt cgccgttatt gtagagtgat
    11221 ttagcattct ta
//
LOCUS       DAYYAO010000088        11203 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000088 DAYYAO010000000
VERSION     DAYYAO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.091"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1424
                     /gene="lysA"
                     /locus_tag="ACHARI_002286"
     CDS             165..1424
                     /gene="lysA"
                     /locus_tag="ACHARI_002286"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8443300.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1411..2211
                     /gene="dapF"
                     /locus_tag="ACHARI_002287"
     CDS             1411..2211
                     /gene="dapF"
                     /locus_tag="ACHARI_002287"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8443301.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2214..3095
                     /gene="dapA"
                     /locus_tag="ACHARI_002288"
     CDS             2214..3095
                     /gene="dapA"
                     /locus_tag="ACHARI_002288"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8443302.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3092..3808
                     /gene="dapB"
                     /locus_tag="ACHARI_002289"
     CDS             3092..3808
                     /gene="dapB"
                     /locus_tag="ACHARI_002289"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8443303.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3814..4509
                     /gene="dapD"
                     /locus_tag="ACHARI_002290"
     CDS             3814..4509
                     /gene="dapD"
                     /locus_tag="ACHARI_002290"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8443304.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4525..5526
                     /locus_tag="ACHARI_002291"
     CDS             4525..5526
                     /locus_tag="ACHARI_002291"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8443305.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5523..6884
                     /locus_tag="ACHARI_002292"
     CDS             5523..6884
                     /locus_tag="ACHARI_002292"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8443306.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6844..7995
                     /locus_tag="ACHARI_002293"
     CDS             6844..7995
                     /locus_tag="ACHARI_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8443307.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7970..9139
                     /locus_tag="ACHARI_002294"
     CDS             7970..9139
                     /locus_tag="ACHARI_002294"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8443308.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHIKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9314..10693
                     /locus_tag="ACHARI_002295"
     CDS             9314..10693
                     /locus_tag="ACHARI_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8443309.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10974..11203
                     /locus_tag="ACHARI_002296"
                     /pseudo
     CDS             10974..11203
                     /locus_tag="ACHARI_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3732 a         1952 c         2497 g         3022 t
ORIGIN      
        1 tattttgttt ttataaccat ttttattctt atttttaaaa aaatatttcg aaatatacta
       61 gacaattcag aaaattttgt ttaatattat tccaataaaa ttcaaatctt tatcaaatga
      121 aattccaata aaaacggaaa aaaagtgaaa agaggaatga aaaaatgaca gaattgataa
      181 ttggcggagt cacagcaagc acattggcag aaaagtataa gacacctctg tatgtgtatg
      241 atgaagaaaa gatgagacaa acgatgctgt cattcaagcg aggctttgta tcgaaacgtt
      301 ttgatacaaa agtgctttac gcctctaaag cattccaaac gattgagatg ctgcatttag
      361 ttcgtgaata tggattcggg ctagatgttg tcagtggtgg ggaaatctat accgctttgc
      421 aagctgattt tcctagagat actatctact tccatggtaa caacaagacg ccagaggaac
      481 tgatctatgc acttgaaaat gatttgttgc atattgttgc cgataatttg atggaagttg
      541 aattattagc agaattaagc caacgctatt taaaaaaaat ctgtatcatg cttcggttga
      601 atgtcggagt agaagcacat actcatgaat atatcgtcac tgcgcatatt gattcaaaat
      661 tcggcatgag ctatgaaagt aaagagtgcc aacaagccct tcagttgata aaagaaagca
      721 gctatctaga gttagaagga tttcatgctc atatcgggtc acaaattttt gatgttactg
      781 cttggttagc tgaaatcgac aaattgacga attacttaac cgattttgac caacctcttt
      841 ccttgaatct tggcggaggg tttgggattc gttatacaca aaatgatcag ccgatcccag
      901 tcgaagaggc actaaaaaaa ctgattgaat atacggaaga agctttggaa aaacgaaaac
      961 tgaccattcg tcaattactg atcgaaccgg gtagaagtat tgttggagaa gctggtacaa
     1021 cactatacac gattggtttc atcaaaaaga caccacataa aaaatattac tttgtagatg
     1081 gcgggatgac agataatatc cgtccagctc tgtatcaggc agaatacgat tgtgatttag
     1141 cagcaaaact gtcggaagaa aaaacagaga aagtgacgat tgcaggaaaa atgtgtgaat
     1201 caggcgatgt ggtaattaaa gaagcttatt tgccgaaagc agaatcgggt gatcttttag
     1261 ctgtttattc cacgggagct tatggatact cgatgagcag caactacaat cgagcgacac
     1321 gaccagcagt tgtttttgtc aataatggcc aatcaagact gattgttcgc agacaagcat
     1381 tttctgatct actaaggggg gaagtttcct atgatccttc ataaatataa tggctgcgga
     1441 aatgatttta tcttattaga ttacgcagaa gacacagatt atagtcaatt agccgcttct
     1501 ttatgcacaa aagaaaaata tgatatagac ggattgatcg ccgtaaaaat cgatccgttg
     1561 gaaatgatct attacaacaa agatggcagc cgcgcgccga tgtgcggaaa tggcattcgc
     1621 tgttttgctc gatacgtaaa tgatcagaac atgatgaatg aacaggaatt caacgtagag
     1681 acattagcag gcacaagaaa aattacggtg ttggatccct caccttttta ttgcagcgta
     1741 gatatgggag tgccagatga ttctccggaa aaaatcaaag ctaatcaaac ttatccaata
     1801 aaaaaccagg aaatagagat caatggtgaa aaagtaacga tcactagttt atttttagga
     1861 acgattcata ccgttgtttt tgttgaaaat gcgcgtgagg aagtctggag aaaaagaggt
     1921 gaactgatct gtcatcatcc tttgttcaaa gaaaaaacca atgtcaactt tgtagaagta
     1981 gtcaatgaac aagaattgat cgtccgtacc tatgaaagag gagtaggttg gacacttgct
     2041 tgcggtacag gttgctgtgc ctcttatgtg gttgctaagg aaactgggaa aatttcagca
     2101 gatcaggtag ttgttcactt ggaacaagga gacttgatga tctctggtga ggatgcgata
     2161 caaatggctg gcccagcagt tcatgaatgg acaagaatat tggaggaatg aagatgataa
     2221 aaggttcgat cgtggcgctg atcacgccga tgaatgaaga gggatcagta gattatgctg
     2281 gtttggaaaa attgatccaa ttccatttag atgaacaaac agatggatta ttagttttag
     2341 gtacaacagg agaatcttct acgctcacac aatctgagga ggagcagatc cttcaactga
     2401 cagtaaagaa agtggctggc cgtgtgccgg tgattgcagg agctggaacg aataatacga
     2461 aagaaacgat tgaaaaagcg aagcattttg cttctttggg ggcagacgca cttttggtca
     2521 tcacccctta ctacaacaaa acaagtgatg caggactagc tgctcatttt acagcaattg
     2581 cagaagcctc accgattccg cttattttgt ataatgttcc ttctagaaca ggcatgtcga
     2641 ttccaatcca tgtattagta aatttagcag aacatcctaa tatcattggt ttgaaagaag
     2701 cctcaggaga tatggcttat gtgatggatg ccgctcggtt gattggagaa gaattcttcc
     2761 tttacagtgg aaatgatgat ttgatcctac cagtaatgag tgtaggagga agcggtgtaa
     2821 taagtgtatg ggcgaatatc caacctaaaa tagtccatga attagtaaaa gatacgcagg
     2881 acggaagatg gcagcaagca aaagaaaagc aattaaatgc acttgaactg atccatgcct
     2941 tgttcagtga gaccaatcct attcctgtca aagcagccat gtcattatta gacttacctt
     3001 ccggacctct acgcctgcct ttagtttctc tttcaaaaga gaaaaagaaa caattagctc
     3061 agcttttgtt aaaagaaagg acggttgaaa aatgaaaatc ggactgattg gcagtggaca
     3121 gatgggaaat agagtcgaag aagttgcaca agaaagagga gatacggtcc ttcttttacg
     3181 gacggcacct aaagaagcaa ctacgcttgc ttttacggaa ctgccagaac tgcttattga
     3241 tttttctcat ccagataatt tagagaaaat cctttcgtat agcttatcat atcaacttcc
     3301 gttagtcatt ggcacgacag gttatacaca atcacagttc caagaaataa aagaacatgc
     3361 caaacatgta cctgtgatgt atagtgctaa tttttcgttc ggcatcatgg tgatgaatca
     3421 actaatcaag caagcagctg caatgttgca aggctggcag atcgaactga ttgaaaaaca
     3481 tcatagtcgt aaaaaagatg caccatctgg tactgcaaat atgctgcttc aaacaatcca
     3541 agaagtgcaa aagctgcagc ccgtatacaa ctggcaaggg aaaaaaagag aagaaaacca
     3601 aattggtgta cacagtattc gtgcaggttc tttacctggt gaacatgatg tattgtttgc
     3661 tgctactgat gaaatattta cgatcaaaca tgaatctttt tctaatcgaa tctttgctgc
     3721 aggcgcagta gaagcagcgg actggttgaa agatcagtca ccaggttatt acaaattaga
     3781 agatttatta atggacaaag gggtgtaaaa aacgtggaag ctcaagaaat catccgctac
     3841 attcaaacgg catctaaaaa gacgcctgtg aaagtctatc tgaatgtaac cgagccaatc
     3901 gaatttaaag aaagtaaagt ttttggtgaa ggaagcagct tcgttgtttt tggtgactat
     3961 gagacgattg ctccggtttt agaagctgaa tcagaaaaaa tcattgaaat cgaaatcgaa
     4021 actgctgcaa gatacagcgc agttcctttg ctagatatta aaaaaattaa tgctcgtatc
     4081 gaaccaggtg ctattatcag agatcaagtg tcaatcggca acaatgccgt gatcatgatg
     4141 ggagcgatta tcaatattgg tgcagtaatc ggtgaaaaca cgatgattga tatgggtgct
     4201 gtcttaggtg gacgagcgac agtgggcaaa aactgccata ttggtgcagg agctgtctta
     4261 gcaggtgtga tcgaaccagc atcggccaaa ccggttatcg tagaagacgg cgtactggta
     4321 ggtgcgaatg cagtgattgt tgaaggtgtc catatcggaa aagatgcagt cgtggcagca
     4381 ggagcagtgg ttttagaaga tgtggcagca gaaactgttg tcggtggaat acctgcacgt
     4441 gtattaaaaa taagtgatga tcagacaaaa gaaaatacag cattgattgc tgcgttacgc
     4501 gaattataga ggagatggag aaaaatgcgc gaaatagata cctgtattgt gggggcaacc
     4561 ggattagtcg gccaaacgat gctcaaagtg ttagaagaat atgattttcc agtaggacga
     4621 ttgaaattat tagcttcaaa acgttcagcc ggtaaagttt gtgtattcaa gaaaaagaaa
     4681 cacactgtac aggaattgac agaaacttca tttgaaggat atgatttagc gctattttct
     4741 gcaggtgctt ctgtatccaa agagtttgcg ccaatcgcac gaagtcaggg actatttgtc
     4801 atagataatt cctcagcatg gcgggaagat tcggatattc cattaatcgt tccagaggtc
     4861 aatctggaag actatacatt gaatcggctg attgcaaatc caaattgttc aaccattcaa
     4921 gcggtacttc cgcttaaagc tttacaagaa gcctttggta tccagcgagt caattattcg
     4981 acctatcaag cagtttctgg ttccgggcaa aaaggcattg aagatcttca tcgtacgcaa
     5041 aatgacgagg aaccagtttt gtatccttat aatattagcc aaacggtgat tccggaaatc
     5101 gatcagcctt tggaaaatgg gtatacaaaa gaagaacaga aaatgataaa tgagacccgt
     5161 aagattctgc atcagcctgc tttgccagta tcagcaacct gcgtacgtgt gccaatcgaa
     5221 aatggacatg gagtctctgt ggcagttcaa ctgcaaaaag agtttacgac ggaagaaatt
     5281 agagagtgtc tcagccagtt tccagggatt caattagaag atgatctagc agcacataaa
     5341 tatccgacga gcattcttgc tagaggaaca gatatggttt atgttgggag gatcagaaaa
     5401 gacacttctt tagagaacgg attgttgttc tatacgactg ccgataacat acgcaaagga
     5461 gccgctgcga atgccgtaca gattgctgca gctttaatgc agaaaatagc ggaggtggca
     5521 agatgagtaa tacgctgaaa gtagcgaaat tcggcggaac atcgatgtct catgaagagc
     5581 aattcaaaaa agtccgtaca attgttcttt cagatcccaa tcgccaagtg atcgttgttt
     5641 ctgctttagg caaacgtacg tcagatgatg acaaagtaac agacttactt tatttgattt
     5701 atgcttacct tcagcatcat gttgattgga cacccctttg gaaaaggatt tgtgatcgtt
     5761 tcattcaagt aagagatgtt cttgccctgc agttacctat tgaagatgaa ctagcacgca
     5821 tacagaaaca actggaagac gaaaagataa ctgaagatta tttagtaagt agaggtgaat
     5881 atctgactgc tcaactgatg gctgagtatc ttggttacca gtttattgac gcaaaagacc
     5941 tgattttctt tgactatgat gggaaaatcg atcaggaaaa aacagcacag gaaatcacaa
     6001 aagcatttaa aagatatccg aaaatggttg ttccagggtt ttacggggcg aaccctgcag
     6061 gaaaagaaaa attattgggc cgtgggggaa gcgacattac cggcgcgata ctggcgaaga
     6121 tgttgcaagc ggaaagttat gagaattgga cggatgtttc aggaatcatg atggctgatc
     6181 cgcgaatcat cgatcatcct aaaatcatcg aggaaatctc ttttagagaa ctgagagaga
     6241 tggcttatat gggggctaac gttcttcatg aagcagctgt tttcccagtt caagaggctg
     6301 atatccccat ccaaataaaa aataccaatg atcctttcgc tcaagggaca aaaatatcga
     6361 atcacgaaat agaaaaggaa aatggactga caggaatagc tggacgcaag gattttcttt
     6421 ccatcacttt attcaaacgt catatgtccg atgaaatcgg gtttatctgg aaagcaatga
     6481 gcatttttgc taaacatgag atatcaatcg aacatattcc ttcagggatc gacaatatcg
     6541 gagtgatcgt ctcagcagaa gcgattgccg ataagttatt tctcatcacg aaagaattaa
     6601 aagaaacact tggcgtggaa gaaatcgtcg tgatcgaaga cttagctttg atctctgtcg
     6661 ttgggggtcc gcataaagaa ttgattggat tatctgggaa agtgttatcg atattgaaca
     6721 aattagaaat tcgcacatcg atcttatcac aaggagcaca ggagttgaat ctgatcatcg
     6781 gtgtaccgaa taatcaatat gaaacagtag tgaagggtat ctatgaaggg atggtgaata
     6841 cggatgctga aagaatatcg aatggcactc catcaaattc ctgaaatcgg gtttcatgaa
     6901 ttcaagacaa aaaaatattt atatgaacaa gtaaaaaaca gcgaggccgt tatccatgaa
     6961 atcggcgaaa caggactact cctttatttt gataatcagc aagaagaaac gattgctttt
     7021 cgaacagata tcgatgcact gccagtcaaa gaagaaacga atctctcttt tgcttctaag
     7081 catgaaggtt atatgcatgc atgtggtcat gacggacata tggcgatgct tttagggttg
     7141 actgacttta taactaaaca cattgaggat cttatttata atgtagtact gatctatcag
     7201 ccatcagaag aaatttccgg tggcgcacaa agcgttattc agtcaggatt gttggataaa
     7261 tatgaagtac aagcaatatt tggctttcat ctatggcctg gactagctaa gggagaagtt
     7321 ttctctcgac caggaccttt gatggcacaa agcagtgaaa cagacattat cattaaaggc
     7381 cagtctgcac atattgcttc tagtgaaaaa ggaatcgata gtctggaggc agctgtacga
     7441 tttttgaaga atgtttatga ttttgaccgg caattaccgg cagagtggaa tcatctaatg
     7501 aaatttggcc aaatccaagg tggaacgatc cgaaatgttt tagctggaga agttattgta
     7561 tccggaagta tccgttctta cagttcagat atgcaaaatt atttgaagag gggattagac
     7621 catttagcag aacagtttca ctctacagct actatttctt ttcgctataa tgacggctat
     7681 ccagctgtaa tcaaccatag aggcttatat gcagcactgt tagattcaaa caaactacat
     7741 gaattagatg ctccagttct tcaagccgaa gattttggca tatatacgca acaatatcca
     7801 tgtgtatttt tctttttagg tgtcggtgat acccctgctt tacatgatcc gacgttcgat
     7861 tttgatatgg atgttttaga aaatggtgta gaatggtaca aaacgattct ttttacagaa
     7921 aatcttcttg taaaaatcga tagcagttca aaagaggagc gagtaaataa tgatgaattt
     7981 atccaatcga tttagtccta ggctttctag aatagaagta tcaaaaatac gacaattcga
     8041 tcagcaaata tcgtcgatac cagatgtgat caaactcacc ctgggagaac cggattttcc
     8101 aactcctgaa catatcaagc aagcaggaat agcagcgatt gaagaagact tttctcacta
     8161 taccggaatg agaggattag aggaactgcg ggaagcggct tgtatatttc agcaacagcg
     8221 ttatggatta acttatgatc cgcaaacgga agttttgacg acagttggtg caacggaagc
     8281 gattgcctct gcgttgttat ctgttttgga agaaggagac aaggttctta ttccagcgcc
     8341 tgcttattca ggatatcagc cattagtgga actagctggt gctgaattga tcccgataga
     8401 tacttctgac acagggtttg tctgtcagcc ggaacaattt gaacgagcat ttgaacaata
     8461 tggatcagct gtcaaagcag tcattttaaa ctatcctaac aatcctactg ggacgacatt
     8521 gtctgcaaaa caattgaaag caatcgctga ggttttaaaa aaatatccgg tatttgttat
     8581 aagtgatgaa gtatatgctg aattgactta tagtggaacg catatgtcca tagctactta
     8641 tcttccagaa caaacaatcg tgataagcgg gttatccaag tcacatgcga tgactggttg
     8701 gcgagtgggc tttatttttg ctcaaaaagc ccttattgac gaactaatta aagtccatca
     8761 atatctggtg actgccgcta caacgatgtc gcaaaaagct gcggctgaag cgctaataaa
     8821 tggggtgaaa gatagcgaga atatgaaaga gcaatatcaa acacgaagag actatctggt
     8881 ccagcaattg gcgcctttag gatttgaagt gacccagcca aacggtgcat tttacttgtt
     8941 ttgcaaactc ccagctgcca ttcaaactga ctcttggcag ttttgtgtag aattagcgga
     9001 acaggcaaaa gttgcgtgta tcccaggaat tgcatttggc ccagaaggag aaggttatat
     9061 ccggatttct tatgctagca gcatggaaaa attacatgag gcctgcaaac ggatcaaaga
     9121 ctttttaaca gaacagtgat tcatagattt tatcaaaaaa acaatcgtta attttgcggt
     9181 atattctgca aggagacgat tgttttttaa atttagttca aatgaacgcg tttcttttga
     9241 ttcataacta atgtaatgtt aagtttaaat tgtttagaat gattttaaaa gaatattcaa
     9301 ggaggtttca tcaatggaaa aaatcaaagt tgtcattgta ggtgcatctc atggagggca
     9361 tcaatcgatc ttggaactat tgacacgata cggtgaaaat gtagatatca cgctatttga
     9421 agcaggagac tatatttcct tcatgtcatg tggtatggag ctgtatttag aagacaaggt
     9481 aacagatgta aatgatgtac gtaatttcag accagaaaac tttccgcaat ccaatgttca
     9541 tattttaaat aatcacgaag taaaaacgat cgatgccgat aaaaaaacag taacggttac
     9601 tagcgcatca gataacgaaa caaaagaata tccttatgac aaattgattt tgagttctgg
     9661 tgtcaaacca aacaatctgc cagtaccagg agcagatcta gaaaatgtct acttgatgcg
     9721 tggatacaat tgggcaacca aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat
     9781 cgctgtcgta ggtgcgggtt acattgggat cgaagcagca gaagcaagcc gaaaagctgg
     9841 gaaagaagtc gtattgcttg atgtgatcga tagaccttta ggcacttatt tagatgctga
     9901 gatgacagat attttagaac aacacctaaa tgaaaacggt attgaagttt tgacaaatgc
     9961 aaaaatcgaa gcttttactg gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga
    10021 aatcccagca gatctagtca ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa
    10081 agggatcgtc gacttagatg aacgtggctg gatccaaaca aatgaatact tgcaaacgaa
    10141 tcttccagat gtgtatgcag taggagatgc tacacttgct tattctatcc cagcacgcaa
    10201 aaaaatgccg atcgctttag cgactgttgc acgtagagaa gcacgttatg tagtaaaaca
    10261 tctttttgaa gaagtaccaa acaaaccgtt cggcggagtc gtaggttcct ctgcattaag
    10321 tgtctttgac tatcattttg cagcaagcgg attgaatagc tttacagcag caaaagcagg
    10381 agtagatgta cgagtgtctt attatgaaga tacgttacga ccaaaatatg taccagaaga
    10441 acaaggaaat ccaaaagtgt ccatccaatt attctcggat ccaatgaccc atcaattgct
    10501 aggaggagca gtattatcta cctatgatgt tacaggtcaa ggaaatgtat tggcactagc
    10561 gatccaaaac aaactgactt tagaagaact agcagaagct gacttcttct tccagccagg
    10621 ctttgaccga caatggagca tgttgaatct agccgctcaa caagcgttag gagaagaacc
    10681 atttgtagaa taagaaaacg aaatatttga aaagaggttg tgaaaaagct gccttgcatc
    10741 aagtaataag aacagccaag taaaaacagc ttcttatgtt tttaacttgg ctggacttat
    10801 taccgcagat gcaagctttt gaacaccgtt tattttcaaa aaagggactg tgacacgatt
    10861 tgtgtaccga cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt
    10921 atggttaaat atacttttga acttaaatta aagatcgttc atgattattt ggatggcaaa
    10981 ggcgattgga ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaaattcggg
    11041 agaagcacaa ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct
    11101 ctatcaataa aaagaaagtt cagcgcttga tcaagaagct aggaataaaa gtaacggcat
    11161 acacgcgtaa atcacgtcgc tacaattctt atcgaggaaa agt
//
LOCUS       DAYYAO010000089        10881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000089 DAYYAO010000000
VERSION     DAYYAO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.092"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..205
                     /locus_tag="ACHARI_002297"
                     /pseudo
     CDS             59..205
                     /locus_tag="ACHARI_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="ACHARI_002298"
     CDS             282..611
                     /locus_tag="ACHARI_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8443310.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            601..888
                     /locus_tag="ACHARI_002299"
     CDS             601..888
                     /locus_tag="ACHARI_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8443311.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1174..2031
                     /locus_tag="ACHARI_002300"
     CDS             1174..2031
                     /locus_tag="ACHARI_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8443312.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            2032..2580
                     /locus_tag="ACHARI_002301"
     CDS             2032..2580
                     /locus_tag="ACHARI_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443313.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2840..3301
                     /locus_tag="ACHARI_002302"
     CDS             2840..3301
                     /locus_tag="ACHARI_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFM8443314.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            3345..3431
                     /locus_tag="ACHARI_002303"
                     /pseudo
     CDS             3345..3431
                     /locus_tag="ACHARI_002303"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3397..4467)
                     /locus_tag="ACHARI_002304"
     CDS             complement(3397..4467)
                     /locus_tag="ACHARI_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8443315.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            5069..6913
                     /locus_tag="ACHARI_002305"
     CDS             5069..6913
                     /locus_tag="ACHARI_002305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8443316.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            6969..8435
                     /locus_tag="ACHARI_002306"
     CDS             6969..8435
                     /locus_tag="ACHARI_002306"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8443317.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            8472..9314
                     /locus_tag="ACHARI_002307"
     CDS             8472..9314
                     /locus_tag="ACHARI_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8443318.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(9531..10826)
                     /locus_tag="ACHARI_002308"
     CDS             complement(9531..10826)
                     /locus_tag="ACHARI_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8443319.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT         3677 a         1712 c         2194 g         3298 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag
      661 aagccattga aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat
      721 aagggtgaac aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg
      781 ggcacaacat atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta
      841 tgtggaacgc atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc
      901 gttcttaaga aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt
      961 cgcgagtatt ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata
     1021 aatcaatata tttcagcttt tccttattta tgtactacct tttacttatt aattataagg
     1081 tagtacatat atatcttttt tttggtagta actaaaaata aatatgttat aatagtatca
     1141 aatgaatatt gctaaaagaa aagaggtagg aacgtggtag cgaagaaata tgtaatcggt
     1201 aattttaaag gtggcgtagg taaaagtacg tgcgcgcaaa tgttagggtt tgaatcagca
     1261 gttaatgaag gaaaaagaac cttgataatt gacttggata tgcaaggaaa tacttctgat
     1321 gtgatgaact taacgcatat gaacttcact aacgaagaag gaggcggaga aggtgaaccg
     1381 attgtttacg aaaatactgt gactgacgtg ttgataaaca atcttccacc taaagaggct
     1441 gtctataaag taatcaataa cctctatata cttccagcag acatgtcctt tgaaatgtat
     1501 gatgattgga tcaaagacca atataatgat tcattatccc aattcaaata tatgaaagaa
     1561 aagctagaac cattatttga tgagtttgac gtcatctatt tagatgtccc accttctatt
     1621 tctgtatata gtaaatccgc gatgtacatt gcggattggg caatcgtcgt cttacagacc
     1681 caagtaaaat cgatgagaaa tgcgatgcaa tatcttgagt atatggactt ttttgtgaaa
     1741 gaatttgata caaatttata tgttgctggt gttattcctt tcatgttgga aagcggtgac
     1801 gcagtagatc aggaaatgta taaacaagcc caagagattt atggggaaca tttgattaaa
     1861 aatgttgtac tcaaaaatgc gcgcttaaaa cgatacgacg gatcaggtat tacgatggag
     1921 aagacaaaga aaggtctctt aaaacaatgg gatcgcaaaa gccacgaatt atttatcagt
     1981 attttgaaag aattggaaga acacgagaat tggtatgtgg aggtggagta attggctgac
     2041 atcaaaggac ttgatatttt tgggaacaac aaaggactcg ccaaaataaa aaaagaagca
     2101 gaagcaaaga aaaagcagca agcaattaca ccatctgaaa ataagcaatt agaagtgcac
     2161 caagttcaag aaataaaaac ttcctccaat gttgaaagac agagtagagg aagaacaaaa
     2221 aaagttggcg caccaatcag aaagtttgat aaggtttatt ccaccaagca tcctttaaaa
     2281 ctgtcgactt tgttaaatgc gacttccaga gttttagtag aaaagtatga gactaatatg
     2341 acaagagatg aattgcttag aaaagcttta gatgagtata ttaaacagaa tcttacgcaa
     2401 gaagataaac aagatttgtt taatgacgtg gtgaaagaac tagacttgtt cagagaaaaa
     2461 tatccaattg ttccagaatt agatgatcgt ggagaaataa taagaactac cagtcagata
     2521 aaacaagata ccgaagactc actgcgcaaa gggtggggaa tgaataataa agttagataa
     2581 taatcaaaga agaactaagt aactaccttt aaacggtagt tacttagttc ttctttgatt
     2641 tgcgtgaaat agaggggatt tatttttctg gttacgtgat taaaaaatcc aaaaaatgag
     2701 cataggaacg gttatgattt tagggtgtgt tttaatgaga tattgtgatt tcattaataa
     2761 gtgtatgata atctgttgtt gagttagtaa ataagtaata gagctaactc taaaataaaa
     2821 ctattaggag gagatgtaga tgaaaaagtt taaaaagaaa atatatttag gattggttgt
     2881 tggggtgagt atgtttgggt tcatggctca tactaaacaa gtaactgcag atactaacca
     2941 aaaagaaagg attatgagcc aattagatga ctctcaaata gagtatgtac ccaatgagct
     3001 aattgtcagt ttaaaggcta cacaaagaat ttcaacagag aaaaataaac cgcaaaattt
     3061 attgggtgta aaagttatgg tggaaaaaga tttgactctt aggacaaatg aaagcgatta
     3121 taataagcga gatcaagtat tattagtgaa attgaacgaa gaaattagtg tggaagaggc
     3181 agtaagaaca tttaatgctt ctgatatggt caattatgca gagccaaact atgtggttga
     3241 actagagccg ttgaatgatg ttgaggagcc aaatacaact gattctttag gaaatcgata
     3301 acgatcctaa ttggagtaaa caagctaatt taagagagat tagcatgcca tccgcttggg
     3361 aagaaaccaa gggttctaag gatgtgaagg tagcggtcat tgattcagga attgattatc
     3421 aacatgaaga tctttctgga aatgtagatg aaagtttggg atacgatttt ttttactttt
     3481 ttcaagaaaa taataattca gaatattctg actatattta tgcaacacta gtgactttag
     3541 gagaaacatc aatagccttg ctaaagtttt tgctttcgct aaagagagta ttttcgataa
     3601 tttgtttttt tctaaaatac ctcgttcctc aatatttggg atatggatat gtatagggat
     3661 tcttcgcata aaggtttcca gaaaatcctc tttgccttct gtagtcgcaa aaactaaacg
     3721 tacattggag gcgtgatgga tagtttcacc gactctagaa taagttccgc catccatata
     3781 aacaaatagt ttctcttgcc cttcaggagg caatctgtgt acttcgtcta aaaacagtac
     3841 tccaccgtct gcatcttgca acaatccatt tcgatcttca tctgcgccgg taaaagcacc
     3901 tttaacatag ccaaatagta tccccgacaa taattcagga ttattatggt attgcgcaca
     3961 attaagtact ttaaaaggag cattctcatg aataacgttc acttttttca taaactgata
     4021 agcagtatga acaaaaaaac tttttccaac tccagtatct ccagtgatca tcataggaag
     4081 ccctcttcca ggatagaaga cagcagtttt catttgttcc accgcatcgg ataagctcat
     4141 atcataccca atcatctttt gtagaatgga atctgtagaa ttgggctcca agtctgtttt
     4201 taattcagat atactttcgt atacatcttt taccggaatc aaaaagtgct cttcaaagcg
     4261 ctctttgatg gcaaataaca ctggacgagt attgatttta aatagaacgt tctttgcaac
     4321 taagtcattg gcaagtttac tggcatttgt tcttcccgtt cttaaaaatt gcgacaggtc
     4381 atttgtgtca aaaaagcact cttctaaatt actgatgcca atttcttcac attttgtgtt
     4441 tgcataagtc actatatcat ttttcatctt ataaacagtc ctttctaaac ttctatccct
     4501 taaataaata gcaaaaaaaa ggaagcaata tcgatatgct gtcctcttaa tttaataaag
     4561 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
     4621 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
     4681 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
     4741 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
     4801 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
     4861 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
     4921 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
     4981 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
     5041 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
     5101 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
     5161 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
     5221 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
     5281 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
     5341 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
     5401 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
     5461 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
     5521 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     5581 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     5641 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     5701 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     5761 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     5821 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     5881 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     5941 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     6001 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     6061 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     6121 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     6181 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     6241 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     6301 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     6361 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     6421 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     6481 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     6541 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     6601 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     6661 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     6721 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     6781 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     6841 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     6901 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     6961 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     7021 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     7081 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     7141 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     7201 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     7261 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     7321 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     7381 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     7441 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     7501 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     7561 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     7621 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     7681 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     7741 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     7801 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     7861 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     7921 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     7981 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     8041 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     8101 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     8161 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     8221 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     8281 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     8341 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     8401 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     8461 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     8521 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     8581 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     8641 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     8701 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     8761 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     8821 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     8881 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     8941 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     9001 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     9061 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     9121 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     9181 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     9241 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     9301 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     9361 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     9421 ttaacgtaag ataataaaaa atctcggctc tttgtcaaat gatgttggta aagagaaatc
     9481 ttagaataat ttggacagaa cagaaagaag aaaacctctt tctgttctgt ttatttggtt
     9541 aaaaatgttt tgccatgacg aatcataatt cgtagtttga aattgtaaaa gttgcgaaag
     9601 ccgtacgcat tgcgttttaa tactttgatg tggttgttta agcattccaa tggtccgttt
     9661 gaatagctgt agtgaagcgc attttggatc tgtggcagaa actttttaaa cgttcgtaaa
     9721 gtggttgtat aggtttctgg cagaccgcta atgtctctag ttaaacaatc ctcaaatagt
     9781 gactgatctc ttttctttac cgcataaaga atgtcttggt ataactgata gccttggcgt
     9841 aagttgggac aagcagctaa cagtcgatcg gcaatctcac tttcagtcaa ataatcttta
     9901 aaactggcac gccaaacccg tttttcatag tttaactttg cttcttcttt ttgtaggagt
     9961 ttccaatagc gcttcaattt tttccctaac cctcgttgag agtgggaatt tcctttaagt
    10021 cgtaccgtgg tttctttcac tcgatgattc aagaaagttc tgccaatatg ttggacgatg
    10081 tggaaacgat cgatgatcaa ctgagcattc gggaaaagct ttttcaccaa cgaaacataa
    10141 ggcgcataca tatcaataac aatcaattga acggcttcac gagtcgctaa tgaaaaccga
    10201 gaaaaatagc gttctaaata aggcaagcgt cgattttcga caatatccaa taatgcatgg
    10261 ctttgtccgt ccatcatgat aaaactcatt gcactggcaa cacttcgaac cgatttgaat
    10321 tcatcaaagc ataagacctt tggcaaagtc tgtttgaaag gattcaatgg gcggtagaat
    10381 cgcttcatga cacgatagac cgaagaagtg gaaatatttt tctgacgagc gatttccgtc
    10441 atggaagtat tctcttttaa gcgagcaatg acagaccaac gaactttttg actaatgaaa
    10501 cagtgttttt cagcaacaga ggtatccgct acgaatgttc tttggcaaga cttacattta
    10561 aatcgttgtt tcttcagctc cagataagtg cgatactcag acacatcatt cagtaacaac
    10621 cgaacggttg tcgtccccca acgaatgatc ctagagggga cgacacaaag acaatgggaa
    10681 cactcttctg gcttgtacag aagagttcct ttgtaaatca agcaaatttg ccctttgtat
    10741 ttttctttag ttaaacaatt ttcctcgaaa tagatattta ggtctagaat atttagggta
    10801 tctttgataa gctgagtatg agacatcttg aatcctccta tgaattggta gccaaattaa
    10861 ttctaaagga tttcagggtg t
//
LOCUS       DAYYAO010000090        10687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000090 DAYYAO010000000
VERSION     DAYYAO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.093"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACHARI_002309"
     CDS             <1..704
                     /locus_tag="ACHARI_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8443320.1"
                     /translation="TNKQRYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKY
                     RPQRSNKPIISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLHTKKII
                     SYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIRHSY
                     SRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSSIHY
                     LTPQEFEELAKEKMA"
     gene            749..1936
                     /locus_tag="ACHARI_002310"
                     /pseudo
     CDS             749..1936
                     /locus_tag="ACHARI_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2056..2256
                     /locus_tag="ACHARI_002311"
     CDS             2056..2256
                     /locus_tag="ACHARI_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8443321.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            2332..2529
                     /locus_tag="ACHARI_002312"
     CDS             2332..2529
                     /locus_tag="ACHARI_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443322.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(2826..4373)
                     /locus_tag="ACHARI_002313"
     CDS             complement(2826..4373)
                     /locus_tag="ACHARI_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFM8443323.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(4457..5260)
                     /locus_tag="ACHARI_002314"
     CDS             complement(4457..5260)
                     /locus_tag="ACHARI_002314"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8443324.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(5263..6045)
                     /gene="phnE"
                     /locus_tag="ACHARI_002315"
     CDS             complement(5263..6045)
                     /gene="phnE"
                     /locus_tag="ACHARI_002315"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8443325.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(6042..6821)
                     /gene="phnE"
                     /locus_tag="ACHARI_002316"
     CDS             complement(6042..6821)
                     /gene="phnE"
                     /locus_tag="ACHARI_002316"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8443326.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(6793..7584)
                     /gene="phnC"
                     /locus_tag="ACHARI_002317"
     CDS             complement(6793..7584)
                     /gene="phnC"
                     /locus_tag="ACHARI_002317"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFM8443327.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(7645..8574)
                     /locus_tag="ACHARI_002318"
     CDS             complement(7645..8574)
                     /locus_tag="ACHARI_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8443328.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            8769..9451
                     /locus_tag="ACHARI_002319"
                     /pseudo
     CDS             8769..9451
                     /locus_tag="ACHARI_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            9612..10655
                     /locus_tag="ACHARI_002320"
     CDS             9612..10655
                     /locus_tag="ACHARI_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8443329.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         3324 a         2169 c         2099 g         3095 t
ORIGIN      
        1 ttacgaataa acaacgctat ggtgccacaa agatccatca agttttatta aaagaaggga
       61 tttcagtatc tcttaaacat gtccaaaagc taatgaaaca attaaattta aggtcgattg
      121 tagttaaaaa atatagacct caaaggtcta ataaaccgat catttcaaaa gagaatctct
      181 taaatcagga cttttctact gaaacgatct gtgagaaatg ggcagctgac attacgtaca
      241 ttcctactaa gaaaaatggt tggtgttact tatcttcaat catggattta cacacgaaaa
      301 aaattattag ttatacattt tcaaaacgaa tgactgtgga ttgtgtcatt caaacattga
      361 ataaagcaaa aatacattat cacattccag aaggaatgat tctacacact gacttgggca
      421 gccaatacac agcaagagaa gtggaacaat ggcttaaaac caacaaaata aggcattctt
      481 atagtagaaa aggaacacct tatgataatg ctggaatcga atctttccat gcctcattga
      541 aaaaagaaga agtctacacg actagctact cagattttga agaagcaaat cgagcgctat
      601 ttagctacat tgaaggattt tataaccgaa atcgaatcca tagctcgatt cactatctaa
      661 ccccacagga atttgaagaa ttagcaaaag aaaaaatggc ttaaccgtct ctactaaaat
      721 gtgtccaaga tattgactca aatccatgat ttacaaagga actcttctgt acaagccaga
      781 agagtgttcc cattgtcttt gtgtcgtccc ctctaggatc attcgttggg ggacgacaac
      841 cgttcggttg ttactgaatg atgcgtctga gtatcgcact tatctggagt tgaagaaaca
      901 acgatttaaa tgtaagtctt gccaaagaac attcgtagcg gatacctctg ttgctgaaaa
      961 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
     1021 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
     1081 gaagcgattc taccgcccat tgaatccttt caaacaggct ttgccaaagg tcttatgctt
     1141 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
     1201 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaatgcta
     1261 tttttctcgg ttttcattag cgactcgtga agccgttcaa tggattgtta ttgatatgta
     1321 tgcgccttat gtttcattgg tgaaaaagct tttcccgaat gctcagttga tcatcgaccg
     1381 tttccacatc gtccaacata ttggcagaac cttcttgaat catcgagtga aagaaaccac
     1441 ggcacggctt aaaggaactt cccactctca acgagggtta gggaaaaaat tgaaacgcta
     1501 ttggaaactc ctacaaaaag aagaagcaaa gttaaactac gaaaaaaggg tttggcgtgc
     1561 cagttttaaa gattatttga ctgaaagtga gattgtcgat cgactgttat ctgcttgtcc
     1621 caacctgcgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
     1681 tcagtcatta tttgaggact gtttaaacag agaagtcagc ggtcttcctg aaacctatac
     1741 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
     1801 ctattcaaat ggaccattgg aatgtttaaa caaccacatc aaagtattaa aacgcaatgc
     1861 gtacggcttt cgcagctttt acaatttcaa actacggatc atgattcgtc atggcaaaac
     1921 atttttaacc aaataaacag aacagaaaga gagtttcttc tttctgttct gtccaaatta
     1981 ttctaagatt tctctttacc aacatcattt gacaaagagc ctgtatttat tactgtcgag
     2041 gacaatggca tcggcatgat tgaagaggaa caaaaaaaca tttgggatcg ctattataaa
     2101 gccgatccgt caagtaaaaa tacaaagata ggcgagtcag gtcttggatt atcaatcgtg
     2161 aaggaactag tgcgcctgca ccacggttca attgaagtga ccagccgaaa aaatgaagga
     2221 acgaccttcc tgatacaatt tccaaaagaa aaataaaatt accagtaaaa aaccgtggag
     2281 agatagctgc tattgatttt tgctatgtct tttctttgag aggtatccgg tatggtggag
     2341 tgtctgaaaa ataaaaaaat ggaggccaat tttgtgaaag ctgttatttc tggaagtatt
     2401 gttttccaag aagaatatcg agatatggaa aaagatttca gtaaaaaagg cattgaatta
     2461 attgattatc ctcgctcatg tccgatccta gacaaagagt atccagaaat cttgacgact
     2521 tttttttaaa tatcgcagca gcggatttat tttttgtatt taatcaagat cggaaagggg
     2581 tattaggcta tattggtgcc gcaacttttt cagaaatgaa gtattgtctg atgcaaaatc
     2641 ttattcatgg aaaaaggatc aaaatagtct tatttaagga accatcagag gaaaattttt
     2701 tgttttgatg aggtagatcg ctggctaaag aatggctgga tggaaatcag acagcctaac
     2761 aatttggcat aacaaaggtg tgggcaaaaa actgctcaca cctttttgaa ttttttattt
     2821 atatactaca aaatcacttg aaaattatga ttcacttttg cttcgatgcg tgggtgaata
     2881 ttcaaatagt cggcaattaa ttcggtcatg tcgatttgga tttctcggac aattttctcc
     2941 gcaccaaaca tcgcataatt tcctccgccg attgcccgat attgatttgt caccacttcc
     3001 agcatttgtt gcggaagaac ggggtgacct ttaaaacgaa gttcggtaat ccgttgtcca
     3061 acaggctgtt tcatatcaat agtgtactga atcccttcat acatgtcata attgtagtac
     3121 tggggcttag gctgaataaa ggctggattg aatacgattt ggttattttc tacagctaaa
     3181 taatttgcgg tctgctccaa cgccgctctc aaatctgcac catttatttg aatcactgcc
     3241 agagtattcg gataaatata gttggtgatg acgtcccgca tcgtgatcgt ctcaccaaag
     3301 ccttttcctt cattattgaa taacgcagtt ccagaaatct ctgagccgct ggcaaataat
     3361 tgtacacgat taataaactc tacataggga tgttcctcaa gacgagcctg catcggatca
     3421 ttaatacgca tatttccgtt cactttacct aagggttgat ccaaccattt ctctagtttt
     3481 tcttctaccg gtacgatcgc tgcaagaatt tctggatcag gctctgattt ttcaactgag
     3541 tgtaaagcaa cctggtgatc tgtaacaacc acttgcccct cttttttctc gacggtaaga
     3601 cgaatttcac cgatgaactc gccacgatag cctggttgaa caacgggaac tccatgaacc
     3661 gtttcagcaa ttttccggtg ctgatgaccg gtgaaaaggg cgtcaatgcc ttcaacctgc
     3721 tgcaatagct cgtagccttc attctctcca gttagcgctt ctgtgtcctc accggttaac
     3781 aagtcttttt caaatccgcc gtggtaacta acgattacca agtctgcttg tttccttaat
     3841 agaggaacat attcttttgc tgtttcgacg atactcttaa acgtaattcc tgctaaggtt
     3901 tctggctttt cccagtgagg aatgtattgt gtcgttaatc ccaagatagc aatttttaca
     3961 cccacctttt cgataatctt ataggcgttt ccaaagtatg gttggtcatt tttgttcacc
     4021 atattggctc ctaatatagg cgcttgataa ttcttaatcg cttcctcgag ataatccaca
     4081 ccatagttaa actcatgatt tcccaacact tgaacatcat agctcatttg gttaatcaag
     4141 tctgtaagct ctttggctga accatattct ttagcaagat aataactcaa aggagaccct
     4201 tggatgaaat caccattttc gatcgtaatt accggtccat ctgcttttgc ccgtaattcc
     4261 ttcagtttcg acgatacttt agccgttcct aaggccagat cggttctgcc ggaatagtct
     4321 gttggtgaaa catagccatg catatcactt gtctctaaaa acgtaatgtt cattctgcgt
     4381 gctcctttag ctgactcaaa atagaaaaac cgaagcgtat tcagctgttc ggtctctttt
     4441 tttgatttat aatgccttaa tccttcattt gaagtgtttt taaaatgttg cctggaataa
     4501 agttttctgt tgtctggcga acaatccttc cttgatcgag atgataaagg ttatacccat
     4561 gacgttcttc aaagcgtacg aagccatcgc cttcatcttc accaacaaag gacatactcg
     4621 atacagtaaa ataggggatc ccattaattt catcagtaaa tgtgtgatgg gtatgaccgt
     4681 ttaggataca gcgaatatcg taaggtgcga ttagcttcaa gaaacgttcg gtaccaggcc
     4741 attttggtat actgctttgg tgagacagta gatgatgatg ggtgaccaag aaaatcggtt
     4801 ggtctttttc ttgctctaag gcttttttca gccattgaaa ttgctgatcg tccactatac
     4861 catctgcgta tccatagctt gaattatcaa aggaaatcac tgtaaactca ggatatttga
     4921 ctatctggtt gtacggggca gaagaggcgg cttcatcgca ccagcctacc caaaaatgtt
     4981 ctttgatatc gtgattgcct agagtaacaa gaatcgtcgt ttctcctagc gcattttcca
     5041 gccaagtctt caagtatcga taatcgtcta tttcgccatc ttcggttaaa tctccgctga
     5101 taatcacaag gtccaagggt gtttgctgca ggagatgatg aatgcattgc tccagaggga
     5161 taaggggatt ctgcatcgta gccagcattc ccttatagcc ttcttcacaa gcctcatact
     5221 ctctgcgaaa gtgtatatct gatatatgca atatattcat tcttatctca atctctttct
     5281 tataattccg gttatttgat ctaaaataaa cacggttacg actacgccaa tcaaaataat
     5341 actcactttg ctccaagacc gagtttgaat cgcgaatatc aatggagcac cgattccgcc
     5401 agcgccaact aagcctaagg tagccgcact acgaaccgct atttcaaaat gatttaatgc
     5461 taaagaacaa taaatcggca tcaaataggg gatgcgaaca aagaaaatcg tttgccaaaa
     5521 atttgctcct accgcatgca tttcttcaac aagtcgctca tccatcgctt ccatttcttc
     5581 tgtaaaaagt ttgccaagca ttccgatctg atggacgcca atcgccataa caccagcaaa
     5641 ggggccaggt ccgacgactt tcacaaaaat aattgcaaaa actagttcag gaaaagctcg
     5701 taaaacatta cagataagct tgccaatttt tgttacccat ggatgtgctt gccagaggtt
     5761 aaccgcactg atcaaggtaa tgggaatcgc taataccgta gcgatcattg tccctaaaca
     5821 ggcgattcca atggttaata atagcaaact aatcaaatcc tcgccgctgc catcatatag
     5881 aaactgccaa tcaggctgac ctaaccctct caaaacgtcc attcccatgg aaggagagaa
     5941 ggtgcttaat ccagagtaat ccagagtagc ggccgacaaa ataacgagaa tcactaaagc
     6001 aactaatgat aaaagggttc ttctgttcat ttcacgaagc atcataacag ggacctcctg
     6061 acacgttcac ttaatttatc aacgatcgcg acagttacta ggatcgttag aataatgatt
     6121 gaaactcgtt cataattcaa aagcgcaagt gaggaattta gaataacgcc aataccacca
     6181 gctccgacat atccaaggac agtagaagca cgaacattca cctcaaacgc gtaaaaagca
     6241 tagctcgcaa actgactcaa aacttgtgga gcgatcgacc aaatagcgat ttgcatttta
     6301 tttgctccaa cagccgcagc ggcttcaatc gggccaaagt caatggtttc aattgcttga
     6361 aacattaatt gtgacaccat accaaaagta aagacagcga tagtcaacac gccagtcgct
     6421 tcaccaattc caaacacagc aacaaaaagc gcggctaaaa gcagggtagg gatggtccgt
     6481 actaaaccta aaagaaaacg aaagatggta gaaagaagac ggttttctgt cactacagtg
     6541 gtcgctaaaa aactaacagg gatcgctaaa attaagccaa tgaccgttcc aagcaaagac
     6601 atttgaagtg ttttcaccat aggctcgact aatttaggta aatagctaaa gtctggatga
     6661 atgaagcgtg ataaaaagac agtcatttga ttggagtttt gaaaaacctg agaaaaatca
     6721 gctgacgtga tccaggcact tccttgaaaa caaacaatta agaacagaag aataagcagg
     6781 tgtttatacc atttaaagtg aacggtttct ttcaagctca tcgatttcgc ctgccttttc
     6841 ttcaaagatt gcattgccat aaatttgctc taagcgttca tcggttaagt cgtctggacg
     6901 accatcaaag acaacctgag cagcttttaa cgcgagcaca cgcgtagaaa actcacgagc
     6961 cagagggact gaatgcagat tgacaatgac tgttttatcc atcgtttgat tgatattttt
     7021 taggtccatc ataacctttt gagtcgtgat tggatctaat gatgcaacgg gttcatccgc
     7081 taaaataatg ggggcttgct gcacgagtgc tcgcgcaatg gatactcgct gttgttgccc
     7141 accactgagc tcatcactgc gtgtctgcaa tttatcagac aaaccaactt tttccagtgc
     7201 ttcctttgtc tgctggtagt cttccttcgt aaaaagacct aaaatacttt tccaagtgga
     7261 gtaatagccc aaacgtccag atagcacatt tttctgaacc gtactacgct ttaccagatt
     7321 gaagctttgc gagattagac caatctggcg gcgaatctgt cgcagctctt ttttattcgc
     7381 ttttgtaata gaggttccat taatgcgaat atctcctttg gtggtttctg ccaagcgatt
     7441 gatactgcgc agcagagtgc tctttccagc accactcaac cctatgatgg aaacaaattc
     7501 tccatcttca attgttaagt caatatcctt caagccaaca acgccattgg gatagacctt
     7561 cgaaacattg tcaaaaataa tcattatcct tctccatttt tttaagtgta agcaaaaaaa
     7621 agcttgctga cacttactga tgttttattc tgctgctttt ttcgtgtagt ctctgatcgt
     7681 gtcataggca gaatcagcag cttcttcata gccctgatgc ccccacaaag ccattgcttc
     7741 tttgccatct ttatcatcct tcatatttaa gaaggtctct ttaattttcg cttttaaatc
     7801 ttttgacatg tcaggctgta cagcgattgc gtcattcgga atatcgcctt ttgtcaggta
     7861 caggactttt aaatccttaa ataaatcatt atctgggaaa ttcgaagcaa aaacatttcg
     7921 cgcgccttca aaaacaaacg ccgcatccat ttgcttgttc aacactgcgg taatctcact
     7981 cggaatatca ttcacagtgg tcatctttgt atctgcagta ggatcaatcc cagcatcttt
     8041 caattcagcg acaggataga tgtagccgct agcagagtta ggacttaagg ttgcgatatt
     8101 ttttcctttt aaatcagtta acttattcaa tccgctatcg gcacgaacca atacttctcc
     8161 tttaaaggtg ttcgtaagtt tgtcctttaa tggcaagcca gtttcttgat cataatcgcc
     8221 gagctgagaa gtcaggattg catctgcagc tccagcatcc tttgcttgaa cataggctgc
     8281 aggcggcata attccgacat cgacttgacc agaagacatc gcttcgacga tcgttgaata
     8341 atcagtagct aacgtcactt ctacctcacg atctaacttt ttcgataaat acttagcaaa
     8401 tggttttgct tttgcctcca tactaccatc attgtttgtt ggaacgaatt gaacaactaa
     8461 tttttctttg ctctcttttt ctgagctgct acttgtattc ccagaaccgc aggccgccaa
     8521 agtggtcaat aacatgactc cgatgaaccc ttttgctaac tgtttgaact gcatttttct
     8581 cctcctgttg gatataattt ctaatacgtc taaatcatag cagggaatca aaatcaatga
     8641 agtgacgcac gtcctatttt acacaacttt tacatacaat ttacaaacaa ttataaataa
     8701 ttattcgtat tgagccgaaa aacaatctaa gctataataa agaaaaactt cttagaggtg
     8761 acgatttcat gaaaaaaatc catgatacga agcaattaac tgcttacgtt gagaagcaca
     8821 acctgcagga ttatatggat tcagatatat tggcaatggc ttctttactt tttttgaaaa
     8881 ggaagagcac ctcattcaaa cgggaaaaac ctcagattat ttgtattttt tagtggatgg
     8941 gacggtaatg gtgtactcgt attcttctga tacacaaaat atttgcatcg attacgcaaa
     9001 gcccgctaca ccactcggcg aagcctcttc attgtgggaa ttatcgccga aaagcagcgt
     9061 taaagcagtg actaagtgca tctgtatcag cattccgcta aatcaatatc gaacacagct
     9121 tcaaaaagat gttcgctttt tgcagaatat ttgccagtta ctaagttatc gtttaaattc
     9181 cgggataaat ttggcgaact cactgacaga acctgttgaa actcgtttag caaagtttat
     9241 tttgacccat cacgaaaacg aacgtttctc atttcggtta accacctgcg cctcaatctt
     9301 aaatgtcagt tatcgacatc ttttacgaac aatcacgaat tttcgtgagg agaatattat
     9361 cgaaaagaaa aagaattatt acttgattca tgatatcgaa gctttagagg acttagctga
     9421 gaatataagt agtccagtaa aagcagaata acagctataa aaaaacagca gtcagaagtg
     9481 attgctggtt ttttgatagt ggagaaattg ggaaacggaa aatcttcgtt gaagagtagt
     9541 gttctatata ttggtatgct atacttttaa aagttcaatg tgtcaagaca cataaaaagg
     9601 ggagaaaaat tttgaagaaa atacttgttt cattgttgtt tttggtgtca ttagtaacgt
     9661 tagtcggctg tagtcagtct cagggggaga agcaaagtga aaaagagaaa agtaaaatcg
     9721 ctttggttct tggatttggc ggggttgatg accgctcctt caatcaagct ggctgggaag
     9781 gattagaggc ttggggaaaa gagaaaaagc tgacagcaaa aaaagatttt acgtatatag
     9841 aaacgaacaa tccagaggat aacagtacca atatcgatca ggcaatcaga aacggcttta
     9901 ccaccattat tagcttgagc tacttgcaag cagaggaact ggaaaatgct gctaaagata
     9961 atccaaagtt gaaatttgcc ttggtagatg gtgagattac tgggaaaaag aatatctcat
    10021 cagcaacctt taaagatcaa gaaagtgcgt atttagcagg actagctgcc gcatatacga
    10081 caaaaagtga tcaagtaggc tttattggtg gaatggaagg gcccgtgatc caacgtttcc
    10141 aaagcggatt tgaagctggc gttaaggcag gggccacagc actgaacaag gaaattactg
    10201 ttaacgtaca atatgctgca tcctatgatt cgctagataa aggaaaagct attgctgctt
    10261 ctatgtacaa agagggagtc gatattcttt atcaggcggc aggaagtact ggagctgggg
    10321 tgttccaaga agcaaaagcc cgcaatgaaa acgactatag cagaaaagta tgggtgattg
    10381 gtgtagatcg cgatcaagcg gatgaagggg attataaaat ggagaacggc gagacaggga
    10441 atttcacgtt gacttctaca aagaaaaatg tcggtgcaga agtcaaagaa tttgcagaaa
    10501 cttctcataa cgataaattt cctggcggca agcatttgac ctttggtttg aaagaagaag
    10561 gggtagatct gacaaaagga caattatcag aggaagccta tcaggcagtt tcagacgcaa
    10621 aagagaaaat catcagtggt gatttcagac tgtagacaaa gtccatttta tggatgatgt
    10681 ctacagt
//
LOCUS       DAYYAO010000091        10650 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000091 DAYYAO010000000
VERSION     DAYYAO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.094"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHARI_002321"
     CDS             33..656
                     /locus_tag="ACHARI_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443330.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHARI_002322"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHARI_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8443331.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHARI_002323"
     CDS             2152..3033
                     /locus_tag="ACHARI_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8443332.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHARI_002324"
     CDS             3036..3806
                     /locus_tag="ACHARI_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8443333.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHARI_002325"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHARI_002325"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8443334.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHARI_002326"
     CDS             5117..6280
                     /locus_tag="ACHARI_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8443335.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHARI_002327"
     CDS             6397..6807
                     /locus_tag="ACHARI_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8443336.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHARI_002328"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHARI_002328"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8443337.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHARI_002329"
     CDS             9380..9664
                     /locus_tag="ACHARI_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443338.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACHARI_002330"
     CDS             9824..10060
                     /locus_tag="ACHARI_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8443339.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACHARI_002331"
     CDS             10057..10455
                     /locus_tag="ACHARI_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8443340.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3722 a         1773 c         2253 g         2902 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt
//
LOCUS       DAYYAO010000092        10408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000092 DAYYAO010000000
VERSION     DAYYAO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.095"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..718)
                     /locus_tag="ACHARI_002332"
     CDS             complement(140..718)
                     /locus_tag="ACHARI_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8443341.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(738..1406)
                     /locus_tag="ACHARI_002333"
     CDS             complement(738..1406)
                     /locus_tag="ACHARI_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8443342.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1421..2491)
                     /locus_tag="ACHARI_002334"
     CDS             complement(1421..2491)
                     /locus_tag="ACHARI_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8443343.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2662..4026)
                     /locus_tag="ACHARI_002335"
     CDS             complement(2662..4026)
                     /locus_tag="ACHARI_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8443344.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(4023..5582)
                     /locus_tag="ACHARI_002336"
     CDS             complement(4023..5582)
                     /locus_tag="ACHARI_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443345.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5638..8118)
                     /locus_tag="ACHARI_002337"
     CDS             complement(5638..8118)
                     /locus_tag="ACHARI_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443346.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            complement(8102..8437)
                     /locus_tag="ACHARI_002338"
     CDS             complement(8102..8437)
                     /locus_tag="ACHARI_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443347.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8451..9485)
                     /locus_tag="ACHARI_002339"
     CDS             complement(8451..9485)
                     /locus_tag="ACHARI_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8443348.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9575..10255)
                     /locus_tag="ACHARI_002340"
     CDS             complement(9575..10255)
                     /locus_tag="ACHARI_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8443349.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2994 a         2119 c         1818 g         3477 t
ORIGIN      
        1 aataaaaata taccctgata atcatgagag aataacaaat ttgtctttat gaaaaactac
       61 ctatataaag aagcaagata taacgatctt cgtcatatct tgcttcttca cctaactatt
      121 ttctccctat ataacagctt tacgcttctc ctaatacacc attcaaatat aaatcgatca
      181 attgcgccac atttagcggt tcttccacag gtcttttcga ccgagtgatg taaggtgcta
      241 acactaagaa aaaatgcctt gctgcgattt cttttgaaat cccatttctt agcacagatt
      301 ggtttgctga aaagatttct tcaaaaggtt cacgataatt tttcttccat agaaagaaaa
      361 gatgatctct tgtttccttt gaaagattgc tttcaagatc gtgaagcatc agaaacaaat
      421 cgaccagatg tgtctcaatc aaaaactgcg ccatttttgt caaggttact tgaaatcccg
      481 tttcgcttgt ttgaacgatt tctctcaaag cttttccaac atcttttaag tggttttcta
      541 aaactgccgt atatagtttt tctttatcac cgaaataatg atagagattg ggctgggtga
      601 tcccaacttt ttgtgcgatt tctcttgttg aagtgccgag atatcccttg gacataaaca
      661 agtcagtcgc ttctttcaaa attttttgct gtgtatcttt gttgtatcga tggaccattt
      721 ttccacctgc tttttggtta ttctttttct ttcaatacac catcttccat cataaatatt
      781 ctatcgcatt tgtcaatcag ccgtgtatcg tgagtaacca tgatcgttgc tttatttttg
      841 tcttttgatt cttttgccaa gatatcgact acttctcgtg ccttttctga atccaaacta
      901 gcagttggtt catctgctag aataatggac ggatcattgt acaatgcacg agcgattgcg
      961 accctttgcc gttctccacc agatagttct tctggatact tgttgatcaa cttatccacg
     1021 ccaagctgct caaataattc gtttgcttct ttttgacgat gttcatgttt aatcttatct
     1081 actaataata actgcttttt tactgtcaga aaaggaacca gattggaagc ttgcaaaata
     1141 aagccaattt ctttaaaacg tagtttaacg cgttcgcttt ctttttgatt actgaattct
     1201 tgatcattga taaatacaga gccactgcta ggcgtctgca atccgcctgc caaagtcaaa
     1261 aaagtacttt tacctgaacc agaaggaccg atgattgcaa caaattcccc tttatcgata
     1321 ctcatggttg tttctttcaa tgctttaact acttggtcgc catcttggaa acttttttct
     1381 acttctttaa attctattgc tttcatgttc ttcctccttc ttatccaatt gccttcaacg
     1441 gatcgatttt tacgattgtc cgaacagaaa atagtgcgcc taacacagct actactaaca
     1501 tcaacaagct aatgacgcca aaaaacagcc aattgctttg gaatggaacc gcatcaggta
     1561 gaacaaggga tgttcccact gttcctaata gaccaagaag aataccaaca aatgataaaa
     1621 tgaatgtttg tgccactaca gaacgcgcaa taaatcctcc tgtgatccct tgtgctttca
     1681 ttacaccaaa gatattaatc ttttgcatcg tcagtacata aatgaaaatc ccgatcacaa
     1741 tagccgcaat aacgatcaaa aatccgatca taaatccaaa agtcaatact tgcgcattat
     1801 atccaggtaa ttcattgatg aatttagaaa ttttgatctg ttctaaatct tcaggtaaat
     1861 tactgatttt acctctgaca actattgcgt taatccgtgc attttcactt gtatcttctt
     1921 tttcgaagcg gatttcttgg taagcattga tggttgtata gagaactggt gagacattga
     1981 atttggcatg atccgtaaag ccagttagtt taaacgtctt gtcactacca gataacttaa
     2041 ctgtatctcc tatggctaga ccatattctt ctttcaaact aatatcgcct actgcctcat
     2101 catcgttatc aaaagctttt ccttcaacaa gattgggcat gatgaattga tttttatcga
     2161 taccgaagaa gctaacattg atttttcctt cttctgtact gtctttacta gtcacgactc
     2221 caggtgtttg ggcaagatac gccactttcc caccttcgac ttcttctgct atcttcttag
     2281 taatcatgga catccctaga tttgaatttg catctttcga aagcacaatc gaatctgcct
     2341 cccacttgtc gactgctgtt cgattatctt gagctaagcc ataagctaac ccagtcaaaa
     2401 aaaataccag gtaggcgatc aaaaacatca cccctgcaac taacgcataa cgtaactttg
     2461 aatgcataat ttcatttaat gctaaaaaca tttaactcct cctcctgaaa taatcatttt
     2521 atacaacaat tttatcactt gataaatttt tggcgaatat tatgctcaaa aaattcttcc
     2581 gctatttcat tatatgaatt ttttattctc gattgattaa tatttattat atccccacac
     2641 aataaaagac cgtgatattt ctcacagtct tttatctact ctattgctat ttgctctctt
     2701 agctagtact ttaaaagcta actcattgag gtagtgaaaa cgagaggtgt attcagacag
     2761 aaatccaaaa tcctcaactg ctagctgtgg aatcgtagtt ttgtgtttca cgatatccgc
     2821 aagcaggttc aaatctgata ataagaattc ttggcttatc agctgtccgc caattatttc
     2881 taatgtatcc ggatggtaga tcagctttgc atttactgtt ttgttttctt cgtcgtattg
     2941 tggaaataga tcaaattctc ctgaacacga accggtattt tgttccaaga atggcgcttc
     3001 atcttctgtc agccctacag ttcctaaaaa gtagtctata atgactgctg ctgttatctt
     3061 ttgtctgagg tcgtaagcgg ttggctgacc actcaaagtt agcgctactg ctcttgccat
     3121 atgtacagct cgagtaacta aaggtaaaaa gagatgccgt ttatttttgt caaaactgac
     3181 tgggacgata tctccaatcg catagatgtc cggatcactt gtctgcagat attcattgac
     3241 tatcacagtc tcatcctcat acaaagccac tttttctttt aacaatgtcg tattaggacg
     3301 agtttgggca gaaaaaatga ccatatctgc ctttattgtt tgagacagaa gttgtacgga
     3361 tactactttc tcttctgatt ttttccaatc gatcaagaat tcatttggat aaaaatttat
     3421 ttgcttagtt tgcagctgat tttgaaaact gttgatcata tccttatcga aatatcttga
     3481 taaaacattc ggcatataat caactagatg aataatgtat cctttatttc tcaaagcatc
     3541 tgctaattcc aatccaatgt aaccagcacc gaagatcact acttcttttg cttcttttaa
     3601 agcggcaagt acttgcttag attgcactag atttttatag gtgtagacat tttcagcttc
     3661 aatttgttta tacaaggtga caggagagga acctgtcgct acgattaatt tatcataggt
     3721 tatggtctcg ttttttcctt ctttggatgc gaggatgagc tgatgtttgt ctggatcaag
     3781 ttcagttact ctccattcgg tgatcagttt tgccccactt tcttgaagag tccgcatgtt
     3841 atgggcaact tcagctaaac tacgtatctt tccttttaaa taaaggttga ttccatttga
     3901 aacatagcct agtccatctt tatgatcttc atcgatcagt aaaacttctg ctttcggttg
     3961 aagttttctt agatttaggg cagctgtgat gcctgcatgg gaagcaccaa tgatcacgat
     4021 tttcatgttt tattcatcct taaattcaat ttttctaaat aacattcttt taaaaattgg
     4081 ttcaaatagg ggaaatcata attatattcg ttgctagtga atacaaaaac tttagcggac
     4141 ttctgtgacg ggtactcttt caattggcta gtcaaaatga tatcatactg cttttctttc
     4201 tcgtattcga ctaaatcata tttgatcgta ttcagcaatt gttcctggat gatttctttc
     4261 acgatatccg ccattagcct ttcataagga agatcataag caatttgcag acgtaattct
     4321 aactgcttga tcagcgtccg taaaatagat gcatagcgag caaataattc aaagcgggca
     4381 ttttcctcgt ctacttcagt aaaatcaaca atagcaaatg tttgtctgac caatctttca
     4441 cataaaacca ggaattgttt atcttctact tcagttaacc gctgttttaa tccttcttgg
     4501 ccaaaaatat tcatccatcc tctaaaatag gcaaaatgag cgtgattttg catcactgtc
     4561 aacgcaatct tttgcgaaat ctcttctgga aaaacataaa aagagaaata ttcttctatc
     4621 gattgtttaa acgttcgatt caattgagct actacgtcgt ctttgtattt ttcgcttatc
     4681 aaaaactcct ggacatgcga gttatgtgta tctaatgtaa agttactgat catgaaaaga
     4741 taaaaaacca acgtttcatt ttgattccaa ggaaaggcat attgaaagaa gtaacgggtc
     4801 aaaaatgtat ggaggtccaa taaaaattgt gtcgtttgaa acaatccaac aggaagattg
     4861 ctataatctg gtttggattt tttcgtacag cgcattcgct tcttcataat ggaaagccat
     4921 atttcaaatt tcaatcgtcc ttcttctgta atagcaaaac taaactcttt ttgcagcaga
     4981 tctaaaaaag tgtttcctga taaatcactg tattcttttt tgttttcaag atagggacgt
     5041 ccaaaccaaa ataattggaa ataaaaataa cgaatttgac tttctggacc ggtcagccgc
     5101 ccgcttttta ttttcagatg aaactcattt aaagaatcat ttaattcctt cactctgcga
     5161 gacaaagtag ccgaactgat attaagttcc atgacaagtt tctgccgatc aaatgagcca
     5221 ttttccaaca gataaagaag tatctgatat tttgtagaat agcgcaagta acaatgataa
     5281 taaacatcga ctgctgctat atttccatgt ctgattaata aaatattccc ttcttggata
     5341 acaatttcca attgtttgat attttccaag ttagcttgta aataaaacag atcttctttc
     5401 aaggtagccc gatcaactga taagcgggta agtacgttat taacggaaac ctctcctttt
     5461 tcctctaata aaagttggaa aagtttgatt tgccgttgtt catttttttc aagcaaactg
     5521 aaaagttcca agtccactcc ttcaagcatt tctgatttta acagcagaag tttccttctc
     5581 atccttgaaa aagaggatct gtgacagtta tcacagatcc atacaaattc taatcactta
     5641 gctggttggc ccagctgtca gtttccatgt gaccatgcct tgatacgttg cgccaggttg
     5701 agcattcgta ctgctattta cctctaagag taaaccatcg gtatcgggaa agtcaacgag
     5761 aacctctcct ttagctgttc catcactctc ataaatcaac gtgttttgac cttgctgaat
     5821 caattgcttt gatccagaat gattgaagta taattttgtt ccgcttaatt cttgaccttg
     5881 atcattctta aataaagaag tcaatgtacc tgtcagttgc caaggagacg tgcttggaag
     5941 ccgagtatcc cgcacatcaa aagaaaaatc gccttttgat tcataataac ttgtcttcaa
     6001 cggtttgggg agatcgttac caaatgtaaa atcatctggt acattaacta atgttaattc
     6061 ttgtttgggg acaagaattg ttttagcaga tgtttggaca actgggttag gaagcttagc
     6121 accatctgct aatgtaatat tgctggaaag tacagctgcc tttgtcagta tgctgacgga
     6181 ttgtaaagaa gtatctacag tatttaattg atagttgtaa gtaagatgaa tggtatctcc
     6241 tattttgctc aatagatctg ctgggacaga aactccttgt tgaatgccgg tatctgcgat
     6301 agttgtacca tttaatttta aggaaccatt gatataagta gtattttcag gaagtgagat
     6361 cgtgattttt ttgtcggtta tcgcatcccc agattttgtt aacgtgaatg tcacattttc
     6421 ttgaatcgta tctcctgata gagcagacag gggatctcct gtctcaggtt gtctgctaag
     6481 gttttttagc tgactatctg cactcacaga tggaaaatta gccactgtta tttgaggaag
     6541 agtggtgatt ccagcttgtt cagactgcca tttgtacgca aaagacggat acatgttagt
     6601 aagtacatcg tccaccaagc gataatcaaa ataatattgc cctgaagcca atgcaggtag
     6661 tgtagaagta gctgtattta cacccccgtt atttgattgg atcgtttgga aaagttgatc
     6721 tgtccatcct gtcgctaatt tttgggttcc tttcgaataa agcctgtaat ggatcgaacc
     6781 cttttttagt tcagttgctg accaattggc cgtgacttgg tacgatgcag aagaaaggag
     6841 ctttccccac tgactatcgg ctgttaagtt tgttggtaaa accgttccgt ctagcttgta
     6901 ctgctccaat acctttaaag caacaggaga aagcaatccg ccttgcgcat ctttcccact
     6961 gtacacgata ttggctgtct gattcaatcc acttgtgctt gctggagtat gagtcagtat
     7021 tgctccttgt gtaacgtttt ttcctgtaaa atagttgact acgcgatcaa agcgactaaa
     7081 tggcaactga tcaaaacgtc cttcagtcag ccccatcgtc aatgtccgac tattggtaga
     7141 gcctggaagg ttctggttat ttccatccac gtattctgtg gtatcagcaa atgtattata
     7201 ttttcctgtt gaatcacttg gtgagatgta actattccac gtatttatct ctgcacttgc
     7261 agaaaatgca tatgctcctg gattcaagtt tccgccagtt aatttaggat aaaatctacc
     7321 tgcagctgga gctaaaatcg aattgaacat acgatcttgg ataatattat tcgaatcata
     7381 aaaatcaaca gaaaaaacgg cctttttaga catagaaggt tgtaatttac ccttggtcaa
     7441 aattttgttt ggactcgtat catatttatc tccgttttct gtcatcttcg cgacattagc
     7501 tgtaacatca aattgaagcg ggatatcgtc cacgttgaat tcgcctcgcg taataatagc
     7561 aataatatct gtcaatagag tatttgtatc ccacgacccc atcccaaaac gcaaggtatg
     7621 gcttcctgct gataataaac ttccgctagt aaccttgatg ggggcaccgg caatatacaa
     7681 gtaggcgttg ttccagtcaa ttgcttttaa tacatcactg cttgatacat gttccggcaa
     7741 attgatgatc gccacaaatg caaatccttg tgcagtgata ttcaccaaat caagtacatt
     7801 tgttagtttc acatggcttt cccaatgaaa acggaccgtg ccacctttcc aaggatcatt
     7861 tgaaccatcg ttcaaataag tagcagtcaa tttaggcaat tggttagccc cagttagttg
     7921 gacaagcttc ccgccattga ctgcactacg tccaccattt ggaggtacga tattttcata
     7981 cgtgtatgga tcataagaaa tggcaggagg cgttgctcca acagcttctg ccgaaggtga
     8041 aaagataaat atacttaaaa caaaaagcga agtaaaaaag aaggagcgaa aaaatgaaaa
     8101 tttattttgt agcaacatgt tcatcctcct tttcctttct tctccataac aacaaccacg
     8161 caagaagcca taattctata ccaatgatca aataaagaaa acggatcttt tgttcattcg
     8221 tctccgggaa tacgccatgt tgattagatt ctttataaat cggtagctgt gtctgctgtt
     8281 ttgatgcgcc attgactgaa ccacttggat gcggaattgg acttagatca taaaatgtaa
     8341 tcgtacctga acttttggta tctgcttgga ctggctcttg ccagctgaag aacagcaaac
     8401 ttgcaataat ccctcccaat aagatcgatg ttttcaacga aatcacctca tcatgttgtt
     8461 tctccttttt tctttttctt gtggacccac aaaaacagca aaatcaacca taacactaga
     8521 actgcagcaa ccaatcctgc ataaagccat gggaaagatt gttcttgtac tgcaaaaacg
     8581 gaattttcat ttactttttg tgctttttct tttttgatcg taaagttttg tgtaaatcgc
     8641 cattcattac tgtcgcctga ttttgctatg atttctgcgc ggtaatcgcc ggcttttgct
     8701 ttatcaccag ctaaatcaag attcaagtga aaattcgaat ttggtgcaac taaaaatgca
     8761 ttagacgtat tttcaatata aagctgattt tcataaaaga tcttgctcgt aaatatcaaa
     8821 ttgctgataa tcctaggctg aggattttgg aaacgaagct gaacagttgg ctttgcgttg
     8881 acctcgtcca aatctcctga taaaagtttc agatccggtt ctatcgtctc ttgactttct
     8941 tgtaaaacga ctgcgagatt ataagcaata cgatttttga tttgtgctcc ttctgtagca
     9001 ttttctgttt cagcttcttc tttaggtgaa acatgaatac ccgctaatac acgtccctta
     9061 aagctttttg ctgggatttt cacttctata ggtacagttg tttctgcatg tgctgataca
     9121 ttgatttcct ttttcgcaat gagcaaaacg tcacggacat caaacggcaa tgtctcatct
     9181 aacgttgggt ttttttgact ataatcgatc gttccaccat cactcgttac cgccggattg
     9241 acagagacct caaatgttcg ttcttcatct gaagaattgc tgataattac ttctagtgtc
     9301 tgattttgtt ccggtgccac taataaatca aaataaccga ttgctttatc tatttggttc
     9361 tctggaaata ccggtttgat tccaaagtct cctgctgaat cctttgcttg gctgattgac
     9421 ggaaaaatga aaaatagact taaaatagaa aaaaacgaaa caaacagtcg tttactttga
     9481 ttcattgtcc accacatcct ttcaacaaaa actgcggtaa aaaatccttc acctatagta
     9541 ttttcaccgc agtttgttga atttatttca tactctatgc tactgggcca gccacgaggt
     9601 tccaagtcaa atcagccgtg aatgttccag ctgctgcttt cggcactttc agtgtgatat
     9661 ttgctggatc ataaacagaa agccatgttc ccagaccttg atcttttgct gctgtaaata
     9721 ttggtgtcgc tgcacttgct ttactcaacc cagttacagt ttgactagtt ggtccagtgg
     9781 attcgttatc tgacgtactt ttaacagtgg atgttccaaa atctaattca gcgccttgca
     9841 aaacttgtga accgtttaca aaatctgaaa ccgaaactgt cacgttccac ccttgtgctt
     9901 ggccatctgc acctacacct cgacgatctg atacttggag ataaggtgta tcgttttttg
     9961 aagcgtctac ttgatacgtt tttgttccgc tttcaatctc gtgtgtacca aatggcaatt
    10021 cgggaactac atccagtgtt aaaggacctg tctggccagt tgctggattt tccggatctg
    10081 ctggctctac tggttttgaa ggatcagtag ggttaagagg ctccacggta tttgttggtt
    10141 cagtaaaggt aatatctcct tttgaagtcg cttgaccctc agcaaaagct ggtgttgcca
    10201 agccgcctag aagaagtgtt gaaagtaata atgtcccaat catgttcttt ttcataataa
    10261 atcactcctt tttcttagtg acctaagtat agtagagaca gtatgcaaaa aatcctcatg
    10321 aattcatccg cttacttgaa attatgaccc tttttgtata aaagagaaaa aaacaataaa
    10381 atcgtttaca tgaatattct tccttttt
//
LOCUS       DAYYAO010000093        10379 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000093 DAYYAO010000000
VERSION     DAYYAO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.096"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHARI_002341"
     CDS             6..305
                     /locus_tag="ACHARI_002341"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8443350.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHARI_002342"
     CDS             378..1739
                     /locus_tag="ACHARI_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8443351.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHARI_002343"
     CDS             1761..2021
                     /locus_tag="ACHARI_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443352.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHARI_002344"
     CDS             2035..2787
                     /locus_tag="ACHARI_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8443353.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHARI_002345"
     CDS             complement(2881..3486)
                     /locus_tag="ACHARI_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8443354.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHARI_002346"
     CDS             complement(3539..4513)
                     /locus_tag="ACHARI_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8443355.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHARI_002347"
     CDS             complement(4891..5610)
                     /locus_tag="ACHARI_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8443356.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHARI_002348"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHARI_002348"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8443357.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHARI_002349"
     CDS             7161..8558
                     /locus_tag="ACHARI_002349"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8443358.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHARI_002350"
     CDS             8639..9079
                     /locus_tag="ACHARI_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8443359.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHARI_002351"
     CDS             9227..9553
                     /locus_tag="ACHARI_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8443360.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHARI_002352"
     CDS             9554..10303
                     /locus_tag="ACHARI_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8443361.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3290 a         1832 c         2151 g         3106 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtct
//
LOCUS       DAYYAO010000094        10227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000094 DAYYAO010000000
VERSION     DAYYAO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.097"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            314..1093
                     /locus_tag="ACHARI_002353"
     CDS             314..1093
                     /locus_tag="ACHARI_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443362.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1139..2083
                     /locus_tag="ACHARI_002354"
     CDS             1139..2083
                     /locus_tag="ACHARI_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8443363.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2099..2878
                     /locus_tag="ACHARI_002355"
     CDS             2099..2878
                     /locus_tag="ACHARI_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8443364.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2890..3588
                     /locus_tag="ACHARI_002356"
     CDS             2890..3588
                     /locus_tag="ACHARI_002356"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8443365.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3616..4380
                     /locus_tag="ACHARI_002357"
     CDS             3616..4380
                     /locus_tag="ACHARI_002357"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8443366.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4424..5875
                     /locus_tag="ACHARI_002358"
     CDS             4424..5875
                     /locus_tag="ACHARI_002358"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8443367.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5907..7043
                     /locus_tag="ACHARI_002359"
     CDS             5907..7043
                     /locus_tag="ACHARI_002359"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8443368.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7073..8149
                     /locus_tag="ACHARI_002360"
     CDS             7073..8149
                     /locus_tag="ACHARI_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8443369.1"
                     /translation="MHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLG
                     GKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRI
                     AHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKFTLIPNAVDAK
                     KFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLI
                     IGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEA
                     QFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKAD
                     YNIQTAYKILESKYYELFELIQRG"
     gene            8155..9177
                     /locus_tag="ACHARI_002361"
     CDS             8155..9177
                     /locus_tag="ACHARI_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFM8443370.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QAMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9199..>10227
                     /locus_tag="ACHARI_002362"
     CDS             9199..>10227
                     /locus_tag="ACHARI_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8443371.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMT"
BASE COUNT         3735 a         1443 c         2098 g         2951 t
ORIGIN      
        1 aactattatt aattcaatgt taattttttt gttattctat tttttgtgta acttttaaaa
       61 acacctatga aaattatgga aataaaaatg aaatcgatta atttttatct tattaatgtt
      121 ttatttactt ttaaatcgta aattgcccta gtcaagaaaa cagtttgtgg tatgattata
      181 tagggaaaac ttgtttacaa atatttctgt gaatatatta gtttaattat gtttttttaa
      241 ataaaaagct gtcattttta tgtaagtgaa ttatatctta atttatctat catcaatact
      301 taggagtgaa tctatggaaa agccattcgt caagtcattg actgctgtgg cggtaggtgc
      361 aggtgctatt gcgatctgtt tgtttggata ccatatcaat aatcagcggc aacatcagca
      421 acggatcaat tatgcagaat ctgccatcac caatcaaaaa gacaccttga cttctttatc
      481 aaaagaagta gacaagctgt atagtacaaa agaaaaaatt tttctaaacc ctgaaataac
      541 agaagaaaca atcacaagtt tgtcaaataa gctatcctct gtcaaacttt cagcagatga
      601 ttacgacatc aaagaatcag aattgcctaa agaagctgtt gcaatccaag atgagaagaa
      661 agcaatcgtg acacagttag atagtgcaga atcaaaactg aagatccaga cagctgtcaa
      721 cgatttattt actaaaaatg tatcaaactg gcagcaagct gtagacgatg tcatcatcaa
      781 agaaaaggtg acaagtgcag atgtcgctcg tgtacgtgaa aatatgagct tttttaaaga
      841 tagtccttgg aaaacggttg ttatgcaata tctaggattc gctgatattc agatcgcgca
      901 agtcacgcaa ctagatcagt tattcgatac gatgttaaag gatggtcaag tgactgccac
      961 cgctacttac gatcaatatt tgacggtgct cagtcaaata gagcagatcc ggaatgaaaa
     1021 aataagcgca gcatacgcaa caaaagcaca aactgttgct gagcaaatgg gctatagcaa
     1081 tataagctat tgacagttaa aacgttgatt caagcaaggt tagcaaacgg aggaaactat
     1141 ggcaagacga agaagaaaat caagagggaa aaaaatattt ctgattattt tagcagttct
     1201 tatcgtatta gtattagcaa ttgtaggagt aggagcaaaa ttatatctgg atctctcaca
     1261 ttctattgag aaaacttacg aatcagtgga acgaacccaa ggagaacgaa cgaaaccagt
     1321 tgatctggac aaacaagaat ctttctctgt tctattgatg gggattgata ctggagattt
     1381 aggtcgggtc gatcaaggaa gatcagatac gatgatggtt gccacagtca gtcctgaaga
     1441 caaacagaca acgatcgtca gcattcctcg tgatacgtat gtcgacatcg taggtcgtga
     1501 cacaaaagac aagatcaacc acgcttacgc attcggcgga gctgccatgg cgatggatac
     1561 cgtacaaaaa tatgtagaca tcccaatcga tcactatgtc tctatcaata tgaaagggct
     1621 aaaggaactc gttgatgcag ttggtggtat cgaagtcaat aatgacctca ctttctcaca
     1681 agatggctat gactttccaa tcggtaaaat tacattgaac ggggatcagg cattatctta
     1741 ttctcgtatg cgttatgaag acccaaatgg tgattacggt agacaagggc gccaaagaaa
     1801 gatcgtagaa ggcgtagcga aaaaagtatt gagtctaaac ggtgtcagca actatcaatc
     1861 cgttttgaat gctctagagt cgaatatgaa aacagacatg aactttgatg aaatgaaaaa
     1921 aattgcttta gattatcggg aggcatttgg tacgattaag caagatcagc tgcaaggaga
     1981 aggcttcatg caagataata tctcctatca aaaagtcagt gatcaagaac ttgatcgtgt
     2041 gaaaaaagaa ttaaaagatc aaatgaatct agagaataaa taggaaagtg gagaaaaaat
     2101 ggaagagaca atcagcttga aagaaatttt tgaaacatta cgcaaacact tggtaataat
     2161 catcatctca atgtttgctg gcttggccat ctcaggaatc gtaacatttt tcatcttgac
     2221 accaaaatac agttcacaag cacaattgat cgtgacactg ccacaatcag acactgcaaa
     2281 tgtcaatgat gtcaatacga acctgcaaat gatcaatacg tataaagata tgatcgtcag
     2341 tgatttagtg ctcaatgaag tcaaagatcg tttagaaaca gaagacaatg tgaaaatgac
     2401 tgctgaacaa atcaaagatg ccatctcagt taatcaggca gaaaattcac aaatgttctc
     2461 gattcaggca ataagtacga atgcagtggc tgctcaacag atcgctaaca caacttcaca
     2521 agttttccaa gagaacgcaa aagatgtgat gaatgttgat aaaatctcga tcatttcagg
     2581 tgcagttgcc aatacgacac ctgtttcgcc aaataataaa ttgaatttaa tgactggctt
     2641 aatgttagga atgatggtag gtgtaggtct ggcattctta ttggaattac tcgatcggac
     2701 agtgaaagat gaaaaatatg tgacagatac attaggattc ccaatcttag gaacagttcc
     2761 agaaatgtca gcgaaagaat tgaatgctgg tatccgtaaa aaaggaccag ctcagacaca
     2821 accaaaagct acaaacgata aaacagcgcc atcacgcaga agccgctcaa gagtctagga
     2881 ggaaagaaaa tggcacgaac acagaaacaa aaagcagccc aaactcatgc agtcagtttg
     2941 gtcacactca ccaatccctc ttcaccaatc gctgaacaat atcgtacgat tcggacgaat
     3001 atccagtttg cctccgcagc tggtcagcaa atcaaaacga ttgttgtgac ttcatcagga
     3061 cctggagaag ggaaatcgac cactgcagca aacattgccg ttgtttttgc caaatctggt
     3121 caacgggtgc tgctagtcga tgcagactta cgtaagcccg tcatctataa aacgttcaag
     3181 ttgaacaacg caagcggatt aagtactgtt cttagttcat caggaagtgt agcagatgag
     3241 atccaacgaa caccagtcga caatttgtcg attctgccaa gtggacctaa accaccaaat
     3301 ccatcagaac tactaagttc gccaagaatg gatcagattt tagcagaagc aagacaacta
     3361 tttgatgtag tgatcttcga catgccgcca gtcgttgccg taacagatgc acagatcatg
     3421 tcttcaaaaa cggatggcac cttattggtc gtacgggaaa atacgtcacg aaaagaatca
     3481 ttgactaaag caaaagaact gttagatatg gtacaagcac gagtcttagg tgtcgtatac
     3541 aacggagcag aacacagcaa agatgctggt tattactacg gaaattaagg aataagaaga
     3601 gaggagacaa gagaaatgat cgatctacat tgccatatcc taccaggaat cgatgacggg
     3661 gcagaaaact tagaagcaag tattgcaatg gctgaaaaag cgattcagca aggcattacg
     3721 catattttat gcacaccaca ccacaataat ggaaaataca gcaatgaaaa atcacaagtc
     3781 atttctctag tcgcttcgtt acaagccgaa ttagagaaaa gacagttgcc tttgacgtta
     3841 ttggaaggac aagaagtacg tattaccgga acgctcatcg aagacatcca ccgagatgaa
     3901 attttattta cagatttaga tgatacatat cttctgatcg aatttccgac actggaagtg
     3961 ccgttatatg cagaacggct tttttttgag ttgttagaac taggaaagat accagtgatc
     4021 gtccatccag aacgcaacgc gcattttcga gaagacccaa atcatttgct tccgttccta
     4081 gatatgggat gtttggccca acttactgcg ccaagttatg ttggtgtatt tggcaaagat
     4141 atccagaaaa cagccaaaac aatggtcaaa cataatctcg tgcagatggc tgcttctgat
     4201 gcacacgggg tctctaagcg caccttttac ttaaaagagt gctatgagca gattgccaag
     4261 gagtttggta aggaaaaagt agtacagatg aaacaagtcg caaaggatct gatcaacggg
     4321 gatgagatca attatccaat ctatgaagag gtaaaaaaga agaagtttgg tttattttag
     4381 ttctttattc gtagataaaa aggaaatgag gagagagtga gagatgtata gaaaaaactc
     4441 aagtggttgg tctaaacata ttgattttat tttattagac ttattttgtg tccagcttgc
     4501 tttttatata tcatatgtaa tgcgacaggg tgactggaat ccgtatgtga ttcctattta
     4561 tcgccacatg gcaattttta tagaacttga gaatcttacg attatatttt tatttgaggc
     4621 atataaaaat gttttaaaaa gaggatatta taaagaggct gtagcttcta taaagcaatc
     4681 ttttatgctg ttacttgtgt gcagtttata ccttattaca atgcaggatg gaaatgatta
     4741 ttccagagtt tctttattta taatgggggt ggtgtatgga ttattaacat atataattcg
     4801 tatactttgg aaagggttgt tgcatcaccg catgaaaaac ggtgaagatg tatcgttagt
     4861 tattgtaaca tctcaaaaga ttgcaaaaaa tgtagttcgt aatcttaaag agaagaatta
     4921 tggcatgtat aaacttagtg gtgtgattgt agtagacaga gatatgtatg gaactaccat
     4981 cggaggtgtc ccagtagttg caaatgctga taatgcagcg gaatatcttt tacagaattg
     5041 ggtagatgaa gtattcatca atgttgacga aagtgttcca tatcccaaag aattaatcgc
     5101 cagatgttcg gaaatgggac ttgctgtaca tatgaatctt acaaaagtgt ctgatagtac
     5161 acgtggaaaa caatttattg gccgagttgg tgattatact gttattacat ctagtattaa
     5221 tgttatgacg atgaggcaag catttataaa gagaacgatt gacattcttg ctggaattat
     5281 tggatgtatt gcaacaggtg ttatttttat cttcgtagca ccagttattt acatcaaatc
     5341 accagggccg attttctttg cacaagaacg tattggtcaa aatggtaaga catttaagat
     5401 gtataagttc cgtagtatgt atatggatgc agaagaacgc aaagcagagt tgatgaaaga
     5461 aaataagatg agtaataatc tcatgtttaa gatggatttt gacccaagaa tcattggcaa
     5521 taaaatactt cctgatggaa caaagaagac aggaattgga caatttattc gatctacatc
     5581 attggatgag tttccacaat tttataatgt cctaatggga aaaatgagcc tggtggggtt
     5641 tagaccttgc ttgaagtcag aatatgagaa gtatgatttt catcatcgtg cgagaattgc
     5701 gatgaaacct gggattactg gaatgtggca ggtgagtggt agaagtgata tcacagactt
     5761 cgaagaggtt gtaaaacttg atgcagaata tatcaggaat tggagtatgg gattagattt
     5821 tagaattttg tttaggacag tcaaaactgt gcttcataga aacggttcca tgtaagataa
     5881 agatactata gaaggagtaa aacgtgatgg caagaaaaaa attaaaaatc tgtatgctag
     5941 gtcataaaag aattccatca agagaaggtg gaattgagat agtggtagaa gaattggcaa
     6001 cgcgaatggt tgcacttggg catgaagtga cttgctataa tcgaagtggt catcatgtta
     6061 gcggaaagga atttgaccag cataaaaata aagaatataa aggtgtaaaa ttaaagacta
     6121 tttttacatt aaatataaaa ggaattgcag caatgagttc ttcagtattt ggtggaatta
     6181 gagcagcatt cggaaaatat gatattgttc attttcatgc agaaggacca tgtgctatgt
     6241 tgtggttgcc aaaacttttt gggaaaagat gtgttgcaac aattcatggt ctagatcatc
     6301 aaagagaaaa atggaacaag cttgctagta catacatcat gttaggggaa aagtgtgctg
     6361 taaaatttgc agatgaaata attgtactta gtgaaagcgt ccaaaattat tttgaagata
     6421 tatatggaag aaaaacaagg tttataccaa atggcgttaa aaaaattgaa ataaaatcag
     6481 caggattgat tacagaaaag tatggattaa caaaagattc ttatattttg tttttggggc
     6541 gattggttcc cgaaaaagga attcggtatc taattgaggc atttaaagat gttcagacag
     6601 ataaaaaact tataatagcc ggaggaagct ctgatacaga tgaatttgca aatgaactaa
     6661 aggaattagc aaaaggtgat gagcgaatta tatttactgg atttgtacaa ggacaggaat
     6721 tagaagaatt atatagtaat gcgtatatct atacacttcc aagtgattta gaaggaatgc
     6781 cattaagttt gttagaagca atgagttatg ggaactgttg tatagtttca aatatctcag
     6841 agtgtacgga agtagtagaa gacaaggcta tgatttttaa gaaatcggat gtaagtgatt
     6901 taaagataag attagaagag gcatgcaatc aatcggaaat ggttaaagtt ttgaagaatc
     6961 aagctactga gtttatttgt agtaaatata attgggataa aatcgtacaa gaaacattaa
     7021 atttgtatag gggaaatata taatgattcg aatattacag tgtgtgaatg atatgcatcg
     7081 tgcagggttg gagacgatgc taatgaacta ttataggaat atagatcgaa ctaaaattca
     7141 gtttgatttt ttgacacatc gtccatataa aagtgattat gatgaggaaa taataagtct
     7201 tggaggaaag gtctattatg caccgagact ttatccacaa aattatccta aatattttaa
     7261 atggatggaa caatttttta aggatcatcc agaatatgag attgttcatt ctcatattga
     7321 tgcgatgagt tatttacctt taaaagctgc aaaaaaagct gggattccga tacggatagc
     7381 acatagccat aatacatcga tagatagaga ttttaaatat cttttaaagc aatattatcg
     7441 atgtaagtta acgtctgttt tgacacatga attttcatgt ggaaaagtag caggtgattt
     7501 tttgtttcga aatgataaat tcactttgat tccaaatgca gtggatgcta aaaaatttta
     7561 ttatgatgaa agtattagaa atgatgtacg aatgaaatta ggaatttcaa gtcagacgtt
     7621 tgtaatggga catattggaa gaatttcata ttctaaaaat catcgttttt tgattgaaat
     7681 ttttaaagaa tttcacgcta ttaagaataa ttccgtatta ttaattattg gaactggtga
     7741 tatggaagaa gaaataaaaa attatgcaaa gaaaagtggc atagatgatg atattaaatt
     7801 tttaggaaat agaaatgata tagaaaaatt ttatcaagca tttgacgtat taatgttgcc
     7861 atcattattt gaaggggttc cgttagtggg tattgaagct caatttgcag atttaccgtg
     7921 tttcttttcg gagaaagttc ctacagaagt tgctttcagt gataagacca atttcataag
     7981 tttaaatcaa tcggcaaaag aatgggcgag agagattgca aatgtagata tctctcatcg
     8041 agattcagaa cgttcgattt taataaaggc agattataat attcaaacag catacaaaat
     8101 cttagaatca aaatattatg agttatttga attaatacaa agaggttaaa gaatatgaca
     8161 cagaaacatt caagtataaa aagaattata catcgcatat ccgaagaatt gaatgcaaca
     8221 agacgaattg caaagataac atcttggaag gaagcatttg ccacgttaca agctaaaatt
     8281 gatattcaag ccatgaatcg taatggatat aatgaaagta aaaaacagaa ggaacattta
     8341 ttaaaaaaac atgaggtaat gctgaagtat tatgaaaaga ctttttcaga ttttttgaaa
     8401 acatatgatt atagtcaaaa tcaaataatt aatcaatcaa aatcaccata ttctaactgc
     8461 atttgggttt gttggtggca gggattggat caatcgccgg aaattgtaaa agcttgtgta
     8521 aaatcaataa aacgaaatgc agggaaacat actgttataa ttttaacaga agataattat
     8581 agacaatatg ttgatatacc aaaatgggtg gaagaaaaga aaaacaaagg aatcattacg
     8641 agaacaaatt attctgattt attaagatta tctttgctag caaaacatgg aggtttgtgg
     8701 ttagacgcca cattttattg tacaaaacca ataccagatg agtattttga tatcgatatt
     8761 tggtctatta ggaggcctga ttatgctcac gcatccgtag catcaggata ttttgcgggt
     8821 tattcattag catgtaatca agagtctaga tggatgtttg ctataattag agactttttt
     8881 ttaaattatt ggaaaaataa tgatatgatg gtggattatt tgatgataga ttatatgatt
     8941 gttttagcac agagaataaa tcatgaaata gcacagacat ttgataaaat acctagtaac
     9001 aatccattat gtgacgaatt gattaaaaat ttaaataata tatatgatga aaaagtatgg
     9061 aacttattaa agaaagatac tttactgttt aagttgacat ggaaacaatc ttttaaaaag
     9121 caaaaagatg ataaagttac tttttatggg atgttggtag aaaacaaatt attataaata
     9181 ttagaggtat aaaaagttat gaaacacaag gtaagtgtga tagtaccaat ttacaatgtt
     9241 gaaaaatatt taagaaaatg tgtggatagt ttattgagtc agacattaaa agagatagaa
     9301 atcgtattag ttgatgatgg ttcgccagat gcttcggggg aaatagcaga tgagtatcaa
     9361 aaaaaatatt ctaatgtaaa aacaatacat agagaaaatg gggggttagg accggcaaga
     9421 aatacaggaa ttgagaatgc gacaggagaa tatgtggctt ttcttgattc agatgattgg
     9481 gtagaaagtg atatgtatga aaaattatac ttagttgcgt ccaaagaaaa tgctgatata
     9541 gtagttagtg gacattgtga ttttgcgaat gacaaagcta tggtgaaaaa gcaacatcct
     9601 ttgagtggga atgtatatga tacgcctaat agtattaaaa atgtacgaaa aaatttattt
     9661 ggacatagtc caaaagatca agaagtagag gccttcccga tgtcagtatg tatgtctttg
     9721 tataataaaa atctgctaga taaatataga ctaagatttg aaaaaatttt atctgaagat
     9781 acaattttta acttgaatgc atatgatgtc gcaaataaaa ttgcttttac agaatataca
     9841 gattattgtt atagaaaaga agaacaagag tctattacaa aaagtttatc tccaaataag
     9901 cagacggaat ttaaagaatt tttaataact ctaaaagcta tagcatcgaa agaagattta
     9961 gaatgtcaaa tcagagcaaa acgtatggca attgattatt gtaggcttta tgtgggctta
    10021 gttgatgatg caaatttggg aattaaagag aaaagaaagt atataaaaaa atttattgaa
    10081 gatgagggat taattcaatg ttggaaggga tatcctacaa atacgctacc atttcaacaa
    10141 aaattttttc ataaaatgct tgaatgtaag tgttatacaa tattgttgtt gttaagtaga
    10201 attagaaaga taattaaaaa tatgacc
//
LOCUS       DAYYAO010000095        10145 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000095 DAYYAO010000000
VERSION     DAYYAO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.098"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..472
                     /locus_tag="ACHARI_002363"
     CDS             <1..472
                     /locus_tag="ACHARI_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8443372.1"
                     /translation="KFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTL
                     FVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLL
                     PKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            481..708
                     /locus_tag="ACHARI_002364"
     CDS             481..708
                     /locus_tag="ACHARI_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443373.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1344..1715
                     /locus_tag="ACHARI_002365"
     CDS             1344..1715
                     /locus_tag="ACHARI_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443374.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1971..2213)
                     /locus_tag="ACHARI_002366"
     CDS             complement(1971..2213)
                     /locus_tag="ACHARI_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8443375.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2245..3147)
                     /locus_tag="ACHARI_002367"
     CDS             complement(2245..3147)
                     /locus_tag="ACHARI_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8443376.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3160..3348)
                     /locus_tag="ACHARI_002368"
     CDS             complement(3160..3348)
                     /locus_tag="ACHARI_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8443377.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3362..3925)
                     /gene="amaP"
                     /locus_tag="ACHARI_002369"
     CDS             complement(3362..3925)
                     /gene="amaP"
                     /locus_tag="ACHARI_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8443378.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3963..4856)
                     /locus_tag="ACHARI_002370"
     CDS             complement(3963..4856)
                     /locus_tag="ACHARI_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8443379.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4934..5857)
                     /locus_tag="ACHARI_002371"
     CDS             complement(4934..5857)
                     /locus_tag="ACHARI_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8443380.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5906..6256)
                     /locus_tag="ACHARI_002372"
     CDS             complement(5906..6256)
                     /locus_tag="ACHARI_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8443381.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6289..7191)
                     /locus_tag="ACHARI_002373"
     CDS             complement(6289..7191)
                     /locus_tag="ACHARI_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8443382.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7184..8041)
                     /locus_tag="ACHARI_002374"
     CDS             complement(7184..8041)
                     /locus_tag="ACHARI_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8443383.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8375..9184
                     /locus_tag="ACHARI_002375"
     CDS             8375..9184
                     /locus_tag="ACHARI_002375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8443384.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9224..9721
                     /locus_tag="ACHARI_002376"
     CDS             9224..9721
                     /locus_tag="ACHARI_002376"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8443385.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         2921 a         1926 c         1750 g         3548 t
ORIGIN      
        1 gaaatttaaa acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt
       61 gttcggcgta cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg
      121 gcagaccaca ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc
      181 ttatttctta ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag
      241 cttaaacata ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg
      301 cttacttcct aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt
      361 aggaatcgct gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa
      421 actgcttgaa catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac
      481 atggcaatta tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa
      541 ttgagtgaga aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca
      601 aaagccatcc gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt
      661 gatttgttga cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa
      721 caaacagtaa ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta
      781 cgtctattat tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg
      841 tactgactca tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa
      901 ctggtttcct gttacgttgt agttggatct gcttttagta taatacttta ttcattccac
      961 cagactgata gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt
     1021 cataccttga agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt
     1081 cttttttagt aagctcccat atatactgta aagccttcta tatattatgt catgttgctt
     1141 tttaacattt actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt
     1201 ttttattttt aacattattt tgataataat gattatttat ttaaaaatac attagataac
     1261 atttgttacg acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa
     1321 ctcacacaat taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg
     1381 tgatctatat tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg
     1441 atacgattgc tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta
     1501 cgagctatta ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct
     1561 ttttcaatct ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc
     1621 atttgtggat catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta
     1681 tctcaagaaa atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga
     1741 cacatttaag gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc
     1801 tgtgacaaaa gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat
     1861 cttcgataat atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct
     1921 tattacttga tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt
     1981 cgagcaaaga atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt
     2041 gccattcctg caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca
     2101 actaaacctg ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct
     2161 cctgcaattg ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt
     2221 tcctcctaat gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt
     2281 ttgtgcgccg cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc
     2341 ggtttttttc gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc
     2401 ttcttttgtt ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat
     2461 attttttact tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt
     2521 gccatattcc acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc
     2581 gatcccagcc gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc
     2641 accgtttgca gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac
     2701 aactttatct tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt
     2761 tccttggtta tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact
     2821 aagtttttct tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga
     2881 aggatcgatt gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg
     2941 tgcatgcttt tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac
     3001 tggatctgga tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga
     3061 ttggtttggt gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt
     3121 gttattattt ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc
     3181 taagattttc gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa
     3241 gatcaataag gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag
     3301 taccccaaag atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc
     3361 cctattctac tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt
     3421 tcaccgatac tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt
     3481 ctttcatcaa tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta
     3541 cattgacggc gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt
     3601 catcttcatg caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact
     3661 ccccatcacc atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa
     3721 gtaacgtgat aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca
     3781 cgtatgaatt agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg
     3841 ttgcacttaa tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc
     3901 cgatcgtttt tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact
     3961 tcttataagt tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt
     4021 gctttattcg atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt
     4081 tctagtaaac tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt
     4141 tgtaatggtg tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca
     4201 agctgttttg ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct
     4261 aataaatgtt cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct
     4321 gctgggatat gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata
     4381 aatgtgtctt tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt
     4441 cctgcgttta gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt
     4501 tctttaggtg cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca
     4561 tctttcacgt attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga
     4621 gcgatatctg ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct
     4681 ccaccagtga ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct
     4741 ccacaatcag gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta
     4801 ggaaatttct ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct
     4861 gtattcctcc tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt
     4921 ttaaatagat tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg
     4981 ctgctatcaa agagccaaag actacgagta taaagcctag agcagaactt ttacccggta
     5041 atcctcctgt attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg
     5101 cagctgaatc tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata
     5161 gatcaccaat actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct
     5221 gtctcaaatg gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag
     5281 aattccataa ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga
     5341 tgtctctttc aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg
     5401 ctttatccaa agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt
     5461 aaatatctaa taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct
     5521 ttgtcagcag ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat
     5581 cggaaagtac aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta
     5641 gattaatcac taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc
     5701 gagaaacttc ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt
     5761 cgattgcttg ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt
     5821 tttcatctgt ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc
     5881 tcctttttgg actttctcga aactattagc tgaagaagca gttctttaat ttgatattat
     5941 aatcgagtac tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca
     6001 tgttcaatcg tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg
     6061 gatgctcgat tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa
     6121 agctgccttt tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg
     6181 cgagtgcgat ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag
     6241 acattgtaat ttccattcct actccccctc ttcagttgtg gtcttctatt atttaacaac
     6301 tacatctgtt tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca
     6361 acggaaagcc gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc
     6421 cttgctccac ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc
     6481 ttcgtattca cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc
     6541 ttgtgcattc caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc
     6601 atcttggtca tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg
     6661 cgtaatattt tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata
     6721 acgcattgat ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc
     6781 aacagctgct acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa
     6841 agcgatcatg tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc
     6901 cggaatcgca atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt
     6961 aaagaattct ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac
     7021 tgagaagcgt aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg
     7081 aacaaattta gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg
     7141 tacgaatttt gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc
     7201 ctactttcgt tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat
     7261 ctttcaacgt attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat
     7321 ttccttcttt atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca
     7381 gcgaagtctg catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat
     7441 tcaaagcatc ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag
     7501 ttttttctgt aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat
     7561 tgaagtacaa gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat
     7621 cgataatagc gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt
     7681 ctagtactaa cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata
     7741 agtttggtaa agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt
     7801 cttcgtagat atctttttcc actgtcagga attggatact tgcattactt gaatatttat
     7861 ttaccgtttc ttccaatcct tctttactat attctgatag accatcgtca atcacataaa
     7921 attttatgcg tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt
     7981 aaggagcata attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca
     8041 tcaaaatcac tcctttttct aatctttttc taacctgttc tacatctaga atattcctca
     8101 tttggtttta aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc
     8161 acaaaacgca tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt
     8221 gaccaaaaac tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat
     8281 aatgacaaag caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga
     8341 aaatagatat gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga
     8401 cggtaccttg cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa
     8461 atttgatgaa aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa
     8521 agatattttt tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat
     8581 tgctgaaatg aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga
     8641 tacactgaga aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg
     8701 agaaacccaa gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta
     8761 ttatgaacat ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga
     8821 tccttctctt agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa
     8881 gctacaaaga gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt
     8941 agaaatcacg cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct
     9001 tggaatcgaa tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt
     9061 gaattacagc gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc
     9121 aaaccaagta acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta
     9181 ttaaaaagaa taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga
     9241 tatgcaaaat catattcttg atccaaacag cgaattttat attccagagt cagatcaatt
     9301 ggcagaacgt attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt
     9361 cacacgggat ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt
     9421 gatccctctt ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat
     9481 tccacctgaa actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa
     9541 agagatcgaa gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct
     9601 gcagagtgct tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa
     9661 acacggagaa tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata
     9721 aaaatatcta tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt
     9781 ttagtcctac gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac
     9841 ggcaggctca cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt
     9901 aacaaacggc aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa
     9961 acgctgattt cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa
    10021 gaacagccaa gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc
    10081 agggcagctt tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg
    10141 ttatg
//
LOCUS       DAYYAO010000096        10083 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000096 DAYYAO010000000
VERSION     DAYYAO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.100"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..2229)
                     /locus_tag="ACHARI_002377"
     CDS             complement(109..2229)
                     /locus_tag="ACHARI_002377"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8443386.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="ACHARI_002378"
     CDS             2459..3637
                     /locus_tag="ACHARI_002378"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8443387.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="ACHARI_002379"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="ACHARI_002379"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8443388.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="ACHARI_002380"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="ACHARI_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8443389.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="ACHARI_002381"
     CDS             complement(4990..6243)
                     /locus_tag="ACHARI_002381"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8443390.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="ACHARI_002382"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="ACHARI_002382"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8443391.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="ACHARI_002383"
     CDS             7821..9167
                     /locus_tag="ACHARI_002383"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8443392.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="ACHARI_002384"
     CDS             complement(9475..9861)
                     /locus_tag="ACHARI_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8443393.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10083)
                     /locus_tag="ACHARI_002385"
     CDS             complement(9910..>10083)
                     /locus_tag="ACHARI_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8443394.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3214 a         1839 c         2054 g         2976 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tgt
//
LOCUS       DAYYAO010000097        10015 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000097 DAYYAO010000000
VERSION     DAYYAO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.101"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(479..703)
                     /locus_tag="ACHARI_002386"
     CDS             complement(479..703)
                     /locus_tag="ACHARI_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443395.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            894..3671
                     /locus_tag="ACHARI_002387"
     CDS             894..3671
                     /locus_tag="ACHARI_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8443396.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3752..4171
                     /locus_tag="ACHARI_002388"
     CDS             3752..4171
                     /locus_tag="ACHARI_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8443397.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4183..4653
                     /locus_tag="ACHARI_002389"
     CDS             4183..4653
                     /locus_tag="ACHARI_002389"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8443398.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4670..5449
                     /locus_tag="ACHARI_002390"
     CDS             4670..5449
                     /locus_tag="ACHARI_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8443399.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5436..6257
                     /locus_tag="ACHARI_002391"
     CDS             5436..6257
                     /locus_tag="ACHARI_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8443400.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6274..7323
                     /locus_tag="ACHARI_002392"
     CDS             6274..7323
                     /locus_tag="ACHARI_002392"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8443401.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7336..8340
                     /locus_tag="ACHARI_002393"
     CDS             7336..8340
                     /locus_tag="ACHARI_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8443402.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8973..9992
                     /locus_tag="ACHARI_002394"
     CDS             8973..9992
                     /locus_tag="ACHARI_002394"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8443403.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3360 a         1652 c         2093 g         2910 t
ORIGIN      
        1 ttcttgcaat cttttttgct acgtaatttt tgtaaaaaag tgggttgaga gaaatctaat
       61 tgattttcaa agaaggagta tgaaaatgag aaaaatattt attcgtttat tgtgggtact
      121 atcttagagg aataaataat agtggagaaa actatggttt ggcgcagatt ctatcaggtt
      181 tgatggaacg aagacaacct cccgacatca agtcaaaagc cacttgagat tttgatctga
      241 aaataaaaaa agaaaacaag gtaggaacat caatgtttct accttgtttt cgtaatcaat
      301 gggaaaaaac taagagtggt gtacgtaatc aataaaaagg atggtgacaa cagtacctct
      361 ttactctctg aaagcatcat aactcgtttt tcctcattat gccaataaat tgttgttata
      421 ccacatataa ggatttgtaa tagctaaaaa cagacaggaa agttgaaatc tgtgaatctt
      481 attttgtatg gaaggattta tagaacgcaa aaatcaagat aaagatgaat aaaaatcctg
      541 tttcggctat atattgcgta tctacgccaa taaaacgagt ttgttctgtc atagccatat
      601 aaagcaaagg caaaaataca actacagcga gataagatcc gatgatttct cgaaaagttt
      661 tccaggaagt atgtttcctg tttttatatt tatccgattt cattgaatag tcatagcctc
      721 cttattcttt catatgttga aattccagta taaaaactgg aaggaactgt acgtttttta
      781 gaataaaaat tataagggat aagtaggctg ctgtctataa taagatatat ttttcaattc
      841 tttattaaag attttattga caaaatgaat ccgttttcta tgatggagga ggggtgaata
      901 agttgttgaa gaaggaaatt ttatgttact tagagaacca gacagccttt ttcgatttag
      961 aacatatgaa cgaggtcttt acagcacaaa ggttagctga tcattttggg gtaaaaagaa
     1021 acacagtcag tcattatttg aaccagttaa atgatgagca actactagtc aaaatcaatt
     1081 ctcgaccagt gatttatttc cacaaagaag cttttgaaaa acaatttttt aagttacgca
     1141 cgaattttta tttttctatc gatgatctca aagaggaaca gccttttttc gcgcaaccaa
     1201 aagacttatt ctcttttatg atcggatacg acgccagttt gaaagaaagt atcgaacaaa
     1261 tcaaaacagc tctttactat ccagatggcg gattaccact tttgattaca ggcgaaagtg
     1321 gcacaggtaa aagttattta gttaatttgg tctatcagta ttgtctgctt catgatctgc
     1381 tagaagattc tgcaccattc atcactttta attgtgcaca atatgcagat aatcctgaac
     1441 ttttgactag caatttgttt ggacatgtta agggagcgta cactggtgca gaagaaaata
     1501 aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt tttagacgag gtacatcgct
     1561 taacaccaga aggtcaggaa aaactgttca cctatcttga tcaaggtgtg atttatcgta
     1621 tgggagaacc taatttgtca cgccggatca aaacacgtct cttttttgct acgactgaag
     1681 agattactag tcatttctta acaactttca ttcgaagaat acctatccga atcgaactac
     1741 ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact gatttactct ttttttctcg
     1801 aagaacaaag aaaaatgggg agccctttgt cagtcagtgg accagctttg tcacttttaa
     1861 caagtcagaa attatccgga aacataggag agctgaaaaa tatcgtcaaa gtttcagcag
     1921 caaaagcata tgctgaacag cgtgaacagc ctgaaatcca tgtcacgatc catcatttgc
     1981 aaaaagaact acttgcgcag cctattacta aaaatacagc acagcaagga atctatatta
     2041 cgcaagacca gaccttagag caattaatgg agaagcaaca gcccgaacag cagcgaatcg
     2101 ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa taactgtctg ctatattctt
     2161 gcgaaggaaa attaaagcaa gaagtgattc aattatttga ttttttgctg tttgagacca
     2221 gtagacaaga aaaacacgag ctgctggttt attggacaca atctatccga gagacctttc
     2281 gacaaatgga aactgcctat caaatcaaat tcgatggaaa cagtgtctat gccttgagtt
     2341 actatctcta tcagcgaagc actatcaaat ggttaccgga agaacatgat attatccaac
     2401 taatcaaaga attagaacaa caagtttctc agtcttatcc atctagttat cactatgtcc
     2461 aacgaattct ggaattaagt aagatcaaat tagatattga agacacaagt atggatcgga
     2521 ttttactgac gatctatcta aaaaaagcga aatggtcaaa agaaaaacga ctgccaaaag
     2581 cgatgattgc tgctcacggt tatgcaactg ctagcagtat ggcaaatgta gtaaatcgtt
     2641 tattacaaga ggatctattt gaatcatttg atatgccact agatgtcaca ccacagcaga
     2701 ttgcttcaga aatccttgat tattgcgaaa acagcgatgt ttcaaatgga ttgatcatat
     2761 tagtagacat gggatctctg aaagaaatcc atcagttttt taaaaaacag ttatccgtac
     2821 cgcttttgat tttgaataat gtgacgacac ctttagcaat cacagtaggg gaatgcctgc
     2881 aaaaaaatgt atcattagaa gagatagcag aagagactgt tcgacagatc cagccagaat
     2941 ggcgactgct ctatccagag gaaaacaaac ccaaagcact cattactact tgcttcactg
     3001 gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa aagcttacct acggcttgtc
     3061 agttgaaaat catcccatat gaatatcagc aattaaaaga taagaaaaac agtgacccat
     3121 tattttccat atacgaagtc gtaggaatga ttggcaccac agatcctgat atcaccaata
     3181 tcgactatct ttcattagaa gacctcatct cgggggaaaa gatgtctgta ctagctgaat
     3241 ggttggacac tactatgaat gcttcagaga aggaaatatt caatcagcgc atcattcgta
     3301 atttttcatt ggaaaaagta ctggattccg tgactattct agatacagag aaggtgatgg
     3361 gagaaatcga tcattttatg agagaattag aggtgcagtt gaatcgaagc atcagcaatc
     3421 ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat tgaacgattg atccgacaaa
     3481 tgccaatcga aacctatcaa ggattagaga aatggaagca gtgtcaaaaa gaagggttat
     3541 ctgctattaa atctgcgttt agtgtcattg aagagcatta tagtgtcatg atccccaatt
     3601 ctgagattgc ctatatctat gatattttga gtgaagatac agagtttctc tcgatagaag
     3661 aagaattttg aacataagca agttgttgcc actaattttg gcatgcaact tgctttttta
     3721 gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga gaaattatct tagcttccca
     3781 tggaaaattt gcttcaggga tattagattc attagagctg atctatggga aaaatcattc
     3841 tattactgta ttcgattgct atactgatga gaattttgat ttagcggaaa cagtaaggca
     3901 gctttttatg aggtacaaag ataaagaaat cattgttctg acggatcttt ttggcggaag
     3961 tgtcaacaac gaattcttac aatatatcaa tcgggatcat gtctatctcg ttgccggatt
     4021 gaatttgcca ctattgattg agctagtatc tcggttggac gctgaagtag agacaactgt
     4081 tttgatccag caggtgctaa ctgattcaaa aaaaatgatc cagttttgta acgagcggct
     4141 caatcagaaa atagaagaag acgaatttta ggaggaagaa aaatgattgt attgacaaga
     4201 gtcgaccatc ggttattaca cggacaagta gctttttcat ggacacaaac aataggcgca
     4261 gactgtattt tgattgctaa tgatgatgta cctacgaatg agatacgaaa gacaacgatc
     4321 aaattagcca agccacaagg agtcaaacta gtgatcaaat caattgatga ctcgattgcc
     4381 gcgttgaaaa gcggggtgac agataaatat aagttattca tcgtagtgga atcgatcgag
     4441 gatgcatata agctggcaga agcgtatcca gaaatcaaaa ccatcaactt aggagggatc
     4501 aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa atattttacc agaagaggag
     4561 gaacgcttga aacgattaat tgacaaacaa atagaagtag aaatccgtca agtaccagca
     4621 gataaaaaag tggatgtaag agaagttttg taagaaagga agaacctcaa tgattgtaca
     4681 agcagtctta ttaggaattg ttgcatttgt cgcacaatca gaatatgcac tagggacatc
     4741 gttgctttct cgtccgattg tgacggggct gtttacagga atcgtgttag gtgatgtaaa
     4801 aactgggatc attatgggcg caactttaga gttagcattt atcggatcgt tctcagtagg
     4861 tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta gggacggctt ttgctattac
     4921 agctggagcc ggtacagaaa ccgcgctttt actaggtttg ccgattgcta cgttgacatt
     4981 gatattgaaa aatatttact tagggctttt gattccaatt atgaaccata aagcagacaa
     5041 ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg cacttgcttg ccggtatcgg
     5101 tctttccttg atgcttggtg ttgtggtaat ggtttcttat atggtgggaa gcaatacgat
     5161 cggaaatcta ttaaatatga ttcctgactt cgttcaaaag gggttgtcag tagctacagg
     5221 gttgattcca gctcttggtt ttgcgatgct tgctcgtttg ctgatcaaca aacaagttgc
     5281 accatatttt ttcttaggtt ttgctatagc ttcttactta gagattcctg tgaccggagt
     5341 agctattttt ggtgcgattt tagcagttgt ggtagtcaat attatgaatg tccgtcaact
     5401 accacttcaa acaactgcag gggaggtaga agaagatgaa gaattctgag atcaaaaaca
     5461 gcaaaataac gaaaaaagag ctaaatcacg tattttggcg ttcttttcaa atggagtttt
     5521 cttggaatta tgaacgtcag atgaatatgg cttatgttta tgcaatgatt ccgattttga
     5581 aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt gaaacggcat ctagagtttt
     5641 ttaacacgac cccacatatc gttacactga ttttaggaat cacggctgca atggaggaag
     5701 aaaataagga tgatcctgaa tttgatgtga ctgcgatcga tagtatcaaa acatctttga
     5761 tgggacctct tgcagggtta ggagattctt tcttttgggg aactttgcga ttgattgcca
     5821 caggtgtggg tacctcttta gccatgcaag gaaatatttt aggccctatc ttgttcattt
     5881 tgattttcaa tattccgcac attctgtttc gctatctagc aacaggttgg ggatatcgct
     5941 taggaacggg ttttttgaaa aagatccagg caaacggtat gatggaaagt ttgacattag
     6001 gcgcgtctat tattggactg atggtcgttg gagcaatgac tgccacgatg atcaccatca
     6061 atattccttt aaaaatcggt tcaggagaaa atgctgtgac tgttcaaagt atttttgatg
     6121 atatcgttcc aaatatctta tgtcttggag catttggcat agtgttttat ctattgaaga
     6181 aagaagtgaa accattaacg atcttgatcg gactagcttt ctttggaatt tttggttcgt
     6241 ggatagggat tttttaaaaa aggagcagag aaaatgttta cgatgcaaga ttatatctat
     6301 gaagaaaagg aagtactaag tacgatcttg aaaaagaatg atttttcgac aagagaacat
     6361 atgaaaaaaa cagtgaattt actgatctta gcaacgggtt catcttataa cgcttgttta
     6421 gcagcgaagc cagcattgga atcttatggt gatcttactg tcgatattca agaacctttt
     6481 tattttttca attatggaaa actttctcct tcaatcgata ctgtgattgc tgtgtcacaa
     6541 agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga tacaaaaaca agggttacct
     6601 gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt tagaagctgc acaaattatt
     6661 gatttaggtg ccggggtaga atcagtaggc ttcgttacaa aaggctattc tgcgactgtt
     6721 ttacagttac tactcttagg gataggaatt ggaataagta aaaataaaat cagcaaaaaa
     6781 atagaacaag actacatgca acagttgcgc aaaataatca accatttacc agcgattatt
     6841 caaaaaacgg aagaattttt tgatggatat caaagtttat ttcgtttagc acagcgtttc
     6901 gtcaccattg gatatggtcc aaactgggga acagctaaag aagcagagac gaaattgact
     6961 gaaacaatcc gcgttccgtc acaaggattt gagttagaag cttatatgca cggcccatac
     7021 ctggaggcag atgcttcgca tttgctattc tttattgaag gggaaagtgt aaataaggaa
     7081 cgttcgcaaa agttgcaaag atatatgagt cggtacgttg gagagactct caccattacc
     7141 acaaaaaaag caagaaacga gaaaacactt ggattagcaa tagaatgtga tgaatatctg
     7201 tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt ataaaaccgc agtagctaaa
     7261 ggtattgact tgaacaaaaa gatttttgaa gactttgata cagtcttgaa aagtaaatta
     7321 taagaggtgg aaatcatgct gaaattcaat gaagaagaac aaatcaaagc aataaaaggc
     7381 gcactagctt tacgtccgca agtagaagaa atcatcgata aactttatgc ggaaaaattt
     7441 gacgctgtct attatctagg aatcggtgga acgtatgctt cctcgatgca agctgtcact
     7501 tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc atgctgcaga atattacact
     7561 actggaaaca aaagattaac aaaagattct atcgttattt tgtcctctgt taccggtaca
     7621 actcaagaag tagtgaaagc agtagaacaa atcaagaaag taggagcgac actgattgga
     7681 tttatcgata aagcgaacag taagttgtct cagctatgcg attttgtggt cacttatcca
     7741 gctcctggaa cagaacaaat caaatttttt atggcagcag atcgattgat gtatttgaat
     7801 ggagaattcg aagcttattc agaatactat gaacagctcg aacgttattt accaacaggg
     7861 ttagtggaag cagagaaaaa agcagatgca tttggcttat catttgcaga aaaacatcga
     7921 cgtgatagta tgcattattt tattggagca gggaatcaat ggggagctgt ttattcatat
     7981 gccatgtgtt actgggaaga acaaagctgg cttccatcga aatctatcca tgctgccgag
     8041 tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc cagtcacatt atttttaggc
     8101 gaagatgaac agagagtgtt atctgaacgt gtggctaaac ttttgccaaa aatttgcagc
     8161 aattatacat tgatagacac aaaagactat ccagtagaag gtatcagtga aaaatatcgc
     8221 ggacgtgttt tgtctttctt attgatgcat gtagtgacac aacgcatcga tgctcatgtg
     8281 gagcagttga actgccatcc attagagata cgccgctact atcgtcagtt tgattattag
     8341 gatagactgg ataaaaagaa tataaaaaat aaagagagca gcgataactg ctccctttat
     8401 ttttttaaaa tattggcatc aattacgtta taaaacacat tttttgcatt atctacagtt
     8461 caacttataa tctgtttaat gggacatgtg ggtgccaacc attttttgtt atgcaatagt
     8521 actatttagt tgttgcatgg atccgtagta aaagtccaag tacaacgagt tgcgccagtt
     8581 tacatagcgt tttgtatcca gaatgttgac taaaacaaaa gttcctatgt caaccgtcaa
     8641 cagagacaat ctgtccgttg caggacctga cagatttaga aatgttcaat ctgtctctta
     8701 tgtattttaa atcaatactc actaggagaa gtttcctaac ctcctaatag taatgctggt
     8761 agtaacattg tcaaaactgc ttcctcttag gaacaagcaa agcataacga gtgtaaaaat
     8821 aaaatgtaga ctattgtaac atatcattga ttatttcaag taaaaacgat aaaagaagta
     8881 aacaaacact taaaaacaat ggaaattagt cccaacttta agaaatgtat actataatgg
     8941 aaattatcaa ttatttattt ggaggaatat ccatgaaaca aaaaatcgca gttcttggtc
     9001 ctggatcttg gggcactgct ttagcacaaa cattggcaga aaacgggcat gatgtccgta
     9061 tttggggaaa tgtacctgaa cagattgatg aaatcaataa gtatcataca aatcagcatt
     9121 ttttacctga tttgacgatc ccagaatcga ttatcggata taaagaattg gcagaagcag
     9181 tggataacgc agatgctatt ttatttgttg ttccaacaaa agcgattcgt tcagtcgcac
     9241 aggaacttat tggtaaaatg aatactaagc caatcattat acatgcaagt aaaggactag
     9301 aacaagatac gcataaacga atctcagaag ttttggctga agaaattcca gaagaaaaaa
     9361 gacaggcaat tgttgtatta tctggtccta gccatgctga ggaagtggca gtacatgata
     9421 tcacaacaat tacagctgca agtattgatc aaaaagcagc agcctatgta caaaaactgt
     9481 tcatgaatga ttatttcagg atttatacaa ataatgatgt gattggcgta gaaactggtg
     9541 cagcactgaa aaatatcatt gcaattggtg ccggcgctat ccatggatta ggattcggag
     9601 atgatgcaaa agcagcaatc atgactcgag gattggcaga aatcagccgt cttggtgtcg
     9661 cgatgggtgc caatcctctg acatttatcg gtttgagtgg tgtgggggac ttgatcgtta
     9721 cttgtacaag tgttcattct cgcaattggc gtgccggtaa cttgttagga cagggacata
     9781 agctggaaga agttctagaa aacatgggaa tggtcgttga gggtgtggca acgacaaaag
     9841 cagcagtaga gctagctcaa cagctaggtg ttgagatgcc aattactcaa acaatctata
     9901 atgttttgta taatggagaa gatatcaaaa aagcagcaaa agaaatcatg ttacgtgacg
     9961 gaaaaatgga gaatgaattt tctcttcgtt aaatagatga tcaatagtta atcgt
//
LOCUS       DAYYAO010000098         9944 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000098 DAYYAO010000000
VERSION     DAYYAO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.102"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACHARI_002395"
     CDS             complement(<1..632)
                     /locus_tag="ACHARI_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8443404.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ACHARI_002396"
     CDS             975..1391
                     /locus_tag="ACHARI_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8443405.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ACHARI_002397"
     CDS             1388..1858
                     /locus_tag="ACHARI_002397"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8443406.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ACHARI_002398"
     CDS             1872..2645
                     /locus_tag="ACHARI_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8443407.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ACHARI_002399"
     CDS             2659..3471
                     /locus_tag="ACHARI_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8443408.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ACHARI_002400"
     CDS             3525..4490
                     /locus_tag="ACHARI_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8443409.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ACHARI_002401"
     CDS             4606..7329
                     /locus_tag="ACHARI_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8443410.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ACHARI_002402"
     CDS             7342..7974
                     /locus_tag="ACHARI_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8443411.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..9700
                     /locus_tag="ACHARI_002403"
     CDS             8873..9700
                     /locus_tag="ACHARI_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443412.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYLGFCCKVLNKE"
     gene            complement(9723..>9944)
                     /locus_tag="ACHARI_002404"
     CDS             complement(9723..>9944)
                     /locus_tag="ACHARI_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8443413.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         3336 a         1526 c         1940 g         3142 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatttaggg ttctgttgca aagttttaaa taaagaataa aatcccttac ggtatctatg
     9721 atttaagctg ggattcccaa taataccttg atttcagtac agaccgaaaa cccgaagaga
     9781 gtgccttctt ttcgggtttt cttatataat cctcgaatgg cttccatgcc tttaatcgtg
     9841 gtagaggcag tgcgtaaact tcgatagaat ttattgcgtc tctttactgg acgatggtct
     9901 tgttcaatca aattattcag gtatttaatg gtacgatgtt ctgt
//
LOCUS       DAYYAO010000099         9869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000099 DAYYAO010000000
VERSION     DAYYAO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.103"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..263)
                     /locus_tag="ACHARI_002405"
     CDS             complement(18..263)
                     /locus_tag="ACHARI_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8443414.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(377..955)
                     /locus_tag="ACHARI_002406"
     CDS             complement(377..955)
                     /locus_tag="ACHARI_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8443415.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1032..1562)
                     /locus_tag="ACHARI_002407"
     CDS             complement(1032..1562)
                     /locus_tag="ACHARI_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8443416.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="ACHARI_002408"
     CDS             complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="ACHARI_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8443417.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2419..2814)
                     /locus_tag="ACHARI_002409"
     CDS             complement(2419..2814)
                     /locus_tag="ACHARI_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8443418.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2811..4223)
                     /gene="cysS"
                     /locus_tag="ACHARI_002410"
     CDS             complement(2811..4223)
                     /gene="cysS"
                     /locus_tag="ACHARI_002410"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8443419.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4220..4756)
                     /gene="epsC"
                     /locus_tag="ACHARI_002411"
     CDS             complement(4220..4756)
                     /gene="epsC"
                     /locus_tag="ACHARI_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8443420.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4842..5081)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5078..6538)
                     /gene="gltX"
                     /locus_tag="ACHARI_002412"
     CDS             complement(5078..6538)
                     /gene="gltX"
                     /locus_tag="ACHARI_002412"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8443421.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6708..7865)
                     /locus_tag="ACHARI_002413"
     CDS             complement(6708..7865)
                     /locus_tag="ACHARI_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8443422.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7899..9269)
                     /gene="radA"
                     /locus_tag="ACHARI_002414"
     CDS             complement(7899..9269)
                     /gene="radA"
                     /locus_tag="ACHARI_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8443423.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9332..9859)
                     /locus_tag="ACHARI_002415"
     CDS             complement(9332..9859)
                     /locus_tag="ACHARI_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8443424.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2506 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa gaaatgcaat aaaaataaat aagaagctca
      361 ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt attttgagtt
      421 ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat tcagccatgt
      481 ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt tttgaaaact
      541 ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaatcgct aaaggttctt
      601 gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg tcgattgaga
      661 cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga gaatagattt
      721 gattgcttat aatgctttta taataacttc caaagctaac acctttgctt ttttcgtaag
      781 ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca gaaacataat
      841 ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg
      901 cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca gacataaaat
      961 aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat cgaactgatt
     1021 agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga caattttttt
     1081 aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat atctttattt
     1141 tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata agcagaagtt
     1201 cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt cgccactgtc
     1261 acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact gtcagccgtt
     1321 tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg ttggatgccc
     1381 ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt cgcataattt
     1441 gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt tttaagctgg
     1501 acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag ctgttttttc
     1561 atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc agcagcaaca
     1621 ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc atccatttct
     1681 tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc aattgatcct
     1741 ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct
     1801 gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac agggacatgt
     1861 tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc tcggtgtttt
     1921 ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg
     1981 tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt ctcttttgtt
     2041 cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag aacgactcct
     2101 tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca tttgactggc
     2161 acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg tgaatcttct
     2221 tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc atggtgccca
     2281 aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg atttcgtcca
     2341 gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg tttcgattga
     2401 tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac accagcggat
     2461 caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga gcgcttcgaa
     2521 ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat ggcttttagc
     2581 atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt cttccagcat
     2641 ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg cacgatgcag
     2701 cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga tttcgtaaat
     2761 cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc tcatgcttct
     2821 cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc tttcaacaaa
     2881 tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg
     2941 atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat accgaagatc
     3001 gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga aactttcggc
     3061 tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat cccatttgct
     3121 gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg
     3181 attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa tttcaaattg
     3241 tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg
     3301 tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca
     3361 acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact cattttttca
     3421 tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa ttttttccct
     3481 gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa atcttgcccg
     3541 ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga acattcaata
     3601 tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc aggttttgct
     3661 tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc ccctgtacgc
     3721 tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc ataattttca
     3781 aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc tttttcaacc
     3841 aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg atgcagtgta
     3901 gcaggctcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa acgatcagca
     3961 acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc gtctacatcc
     4021 gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg tcgaattgta
     4081 tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac agtcggtccg
     4141 cagacataca taccgacatg tcccttttgt atagggatga attcttcttt ttttctggtc
     4201 aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg attcgaacga
     4261 cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac acaacggcac
     4321 ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc gcattcgcag
     4381 agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt cctgtgccgc
     4441 ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga
     4501 cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct ggatggatct
     4561 caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa aagagccggt
     4621 gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca
     4681 aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc
     4741 gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag gaaatcatct
     4801 tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac gctttccgcg
     4861 tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct gttatacgtt
     4921 cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc gccaagacca
     4981 ctcccagagg catttcggca accattcatt gataccttgc accacccggt atctctcttg
     5041 taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact tgatttaaat
     5101 gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct ggaccatgca
     5161 tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt acgccagttt
     5221 ctttttgaac agctttgatg gcagctttga cagttggagc atctactact tccatttgtt
     5281 ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca agtacttctt
     5341 ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga tcaacgattt
     5401 ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc cattcgattg
     5461 tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc agacacatgt
     5521 cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat tgcagttttt
     5581 tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt ttgatcaatt
     5641 cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat gcaataaagt
     5701 tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat tgcaagattg
     5761 attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat gtcatatgtc
     5821 cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt ggtgtattgg
     5881 caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca tcgaccgcaa
     5941 cagcaaaatt ataagttggc ataccatcgc gtttttgtat gacaaagtcg ccaccgatat
     6001 tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat tctgtattgc
     6061 gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct gcttgttctt
     6121 caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg
     6181 cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct cgattgctag
     6241 caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga cggtaagggc
     6301 cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa gcaaggtttt
     6361 ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa
     6421 taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca gttcttgcat
     6481 ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact ttcgtcattt
     6541 tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca gttacggctt
     6601 ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta aggtaaatag
     6661 taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta ttttgattgt
     6721 ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg tgcaggttta
     6781 gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac ttcgatacgt
     6841 tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc aaggtaggct
     6901 actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt ttcaccaggg
     6961 atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg tacattttgg
     7021 aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc taaaagctta
     7081 gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc atcgtacatt
     7141 tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc gcgacgacca
     7201 cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa
     7261 ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata aatccgaccg
     7321 tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc tgattttcgt
     7381 tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt gcttcgtgaa
     7441 ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc cagatagcct
     7501 aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc catattgtaa
     7561 agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat cgcaccgaag
     7621 agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat aatagctgag
     7681 atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa caggctgttg
     7741 gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca agctgagggt
     7801 aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat gacacgtttt
     7861 tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac acgcttcaac
     7921 gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc ccagttacct
     7981 aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc tttgactcgt
     8041 tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc gatgaaacat
     8101 tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc gatagctaaa
     8161 tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc atcttgattc
     8221 tgaagaagca acccagctct tttttccagc actgccatga tcagagatac ccgattgaaa
     8281 tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac taatgcttgg
     8341 atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga gccagttgca
     8401 ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc caatccatgc
     8461 tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt tactgctcgc
     8521 aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc caccatatgt
     8581 tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac gataaaaatg
     8641 gctatcccat ttgttttagc gattttgagt agttctgcag tggtttctct tacttggctg
     8701 acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga atcaatgata
     8761 acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc tgtttcagcg
     8821 tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt tatctgctct
     8881 gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa ttgctgagaa
     8941 acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa gaccatagac
     9001 cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt tttgatccga
     9061 ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa
     9121 cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat cgtattccat
     9181 tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca attttggcaa
     9241 acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct aatcatgaga
     9301 cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca aatccgcctt
     9361 gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag atcccttggc
     9421 cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg aattggaaca
     9481 tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca cctactccgt
     9541 ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt
     9601 catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc aattgttttt
     9661 cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc tttttccagc
     9721 ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca tagccagcag
     9781 catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta gaaataactt
     9841 caaatcctct ttttctcacc ggttttcct
//
LOCUS       DAYYAO010000100         9399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000100 DAYYAO010000000
VERSION     DAYYAO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.104"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            586..1821
                     /locus_tag="ACHARI_002416"
     CDS             586..1821
                     /locus_tag="ACHARI_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443425.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1905..2993
                     /locus_tag="ACHARI_002417"
     CDS             1905..2993
                     /locus_tag="ACHARI_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFM8443426.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            2990..4411
                     /locus_tag="ACHARI_002418"
     CDS             2990..4411
                     /locus_tag="ACHARI_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8443427.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            4447..5163
                     /locus_tag="ACHARI_002419"
     CDS             4447..5163
                     /locus_tag="ACHARI_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8443428.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            5182..6765
                     /locus_tag="ACHARI_002420"
     CDS             5182..6765
                     /locus_tag="ACHARI_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFM8443429.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            6894..7478
                     /locus_tag="ACHARI_002421"
                     /pseudo
     CDS             6894..7478
                     /locus_tag="ACHARI_002421"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            7424..7942
                     /locus_tag="ACHARI_002422"
     CDS             7424..7942
                     /locus_tag="ACHARI_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443430.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            7965..8990
                     /locus_tag="ACHARI_002423"
     CDS             7965..8990
                     /locus_tag="ACHARI_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8443431.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            9239..>9399
                     /locus_tag="ACHARI_002424"
     CDS             9239..>9399
                     /locus_tag="ACHARI_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8443432.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         3413 a         1040 c         1740 g         3206 t
ORIGIN      
        1 agttacaaaa aaatattata aagaataata ttttctaaaa agtaaactat agtaatgtgg
       61 attatcacct tccaacagta tataaaatga gcgtaaaaac ggctattttt gccttaacaa
      121 cgattttttt gtatacaaat acgatatgct cattaattat tggaatttgt agtgctgttt
      181 aaattaatga aggtgtaatt atatgagtga tcattttgaa aaagtttgtt gagaagatgt
      241 gcttctagtc atgtgatggg agaaaataat ttaactgtta gagtgaatag ttaaattatt
      301 ctggttatat gaaaaaagta gtttctaaaa taatcttgca gggattaact taaagaaatt
      361 gagagaatag tttctttgaa atttctatat acaatgagaa gctgaaattt acttttattt
      421 gatgactgga aatagaaatt aaatatattg tattgttaca ttaaagaaaa cttgtatgga
      481 aaaatcagta caataccatt atttaatgtt cttcacttaa agcgcaaaaa atatcagttt
      541 attaaactcg tatatagaac aaatatatgt agacggggcg aaacgatgag tgtaaaaaaa
      601 atgctattta tggtaagtac aataattgta ttaacatccc gattttgggg aattaatact
      661 agtttatcta tgcgatattt tattatggca atatgggatt tatattttat gttaagctat
      721 ttttcttatc gtgagaaaac atataaaaaa tacgttatca agaataactt ttggttgact
      781 ataatgccgt ttgtgatatt ttctatctat actctaataa tatggggaat tggttcaaat
      841 gcagaatttg aaaattttat aaagttatgt tccacgttgc tttatttaat tcttgcatat
      901 ggttacgcat gtacagcatt ttacttattt aaggcagaag gaattgattt tattttttgg
      961 tcaggtgtta tttcatatat gagtggttca gttttttatt taattgtatc ttatggttta
     1021 aatggtttaa tttcttatat aaaaagtctt ataacaggtg aaacaaatac tgcaaatttt
     1081 gctatggagg tacatgattt aacttttgcg atgggggttt ttttccttta ttacgtattt
     1141 tttgaaaata aaaatacaaa aaaacataag ataaaaatta tcgtatcttt aattttaatt
     1201 ttcatgggat tgaagagaat tgaactgtta gctttaagtg gtgcaattct agtgtattat
     1261 gttttattaa agtggggaaa aaagattcaa atccgtgcat tagtgtgtac tatttgtgca
     1321 actgttattt cgttagtgtt tatttacatt atagatacag gaattttggc attgcttatg
     1381 tcaaatttgg gcattacaga tgacggtgca aggctttcat tttatctata ttcatctaaa
     1441 ttttatgaac ttggtttagg atatgtagga acaggatgga catggttttc aaaatacttt
     1501 tttaatcttt atttatcgca ctttaggtta aatggtggaa gaattgcagc aagcctacac
     1561 agtgatatat taactattta tatagaagtt ggatgtatag tttttattat atggatgttt
     1621 tggttatttt ttgtgaaacc gataagacta actaaaaaat ttggattgaa aacaggggag
     1681 tgtgtattat tattaactat atatatgttt atattatatt tgacggataa tacgctcact
     1741 tatccagaca ctcaaatgat ttttctctta gtacctatga tagtttcgtg ggttgataat
     1801 caacaacgaa tatcggaata gatagctaaa gtatatttta attatgaatt tataattagt
     1861 tgtaaaagtt aatatttaac ggacaggaag tagggtgaaa aaatttgaaa aaaattttag
     1921 tgttagcaaa tacatattat caaataatag tagcaataca aatgaaaaat acaatttttt
     1981 ccgatgagta tgttgaaatt attatttctg atcattctaa aaacactaaa gaaattgcga
     2041 aaaaaataga agaaaaaaaa gtgtttgatg gctgtcatta tttagaaagc aaatcttttg
     2101 atgataatat tgggtatttt caaaagatta aggactgtct tcagatttct tgtggtaaat
     2161 caaatcgata tgcctattat ttgaaaaact gtaaaagtga atattttgat gaatttattt
     2221 gtttttcaat gggagaggtt tggttggaag gaatatatgc ttgcttatat cgaaataata
     2281 aaaatgtaca ggtatcaatt ttagaagaag gtattttaag ctatggagtt gacattattc
     2341 caacagcaag aacaaaagct atcgcagtac tcaattactt aaaaaaaagg actccactat
     2401 ataacaaaag ctataatttt tattgttttt ttcctaatat atatacagga agagagaata
     2461 caatacaaat tccattagtt acagctgggt caagaaccgc attgcaactt tcggatattt
     2521 ttaaccctat gatagatatt tataaagaaa aaaaatatat attttttacg tctgtttatg
     2581 attttgaggg cggagaacct gtcggagaat atgaattagt atgcaaaata gcagacttag
     2641 taggaaaaga taatctaatt gttaagacgc atcctagaga tgtaagaaca atttataaag
     2701 ataatggatt tatcgaggat aaaaattcat caataccttg ggaggcgatt caattgtcag
     2761 gcgattttaa cgataaaata tttatgacaa ttaattcagg aagtgttatt tcaggtagta
     2821 caatgtctgc aaaacctgta cgcacgtatt atatgtataa attgtgtaac atagaaggaa
     2881 atccgtcttg caaaaagaat gcgtatgata ttgagcgatt gcttaagcaa aaaacgatga
     2941 aaaacatttt aaagaatgtg aaaatagcag aaagcttaga tgatatacta tgagcaaaac
     3001 taaatattta attaaaaata tgggaatatt aacaataagt aatttttctt caaaaatttt
     3061 agtttttttg ttagtccctt tatatacaag tgtattatcc acaacagaat atggcacata
     3121 tgatttgaca atttcaacag ccacattatt gtttccaatt ttaaccttaa acattgtaga
     3181 tgctgtgatg cgctttttaa tggataaaga taaaaataaa aaaaatatag catctatagc
     3241 gattaaagtt atatttttaa gcgtgatagt ctttggatcc gtaatgtatt tgttaaatat
     3301 tacaaaatta tggcaagatg tttatggatt agaaaaatat atttttttgt attatatatc
     3361 ctatgtatgg aatcaatttc taatacaatt tgctaaaggg ttagaaaaag ttaaagctat
     3421 gggcatagca ggtattatca gcacaattgt aacaataatt gccaatattt tcttcttgct
     3481 tattctgaaa tggggattaa ttggtttttt cattgcaaat attttatcac agattatttc
     3541 agctacttat ctgtcagtat tagtgagaat atgggaatat atcgattttg aggctaaaga
     3601 tcctcagcta aagagagaaa tgctaaatta ctcgattcca ttaattgcaa ctgttgttgg
     3661 atggtggatt aatagtacag ctgataggta tgtggtttcc tttatagtag gtgtctcggc
     3721 gaatggctta ttgtctgtgg catataaaat acctcaaata attaatactt tgcagggaat
     3781 atttatacag gcctggcaaa tttctgcaat taaagaatat ggagataatg agactgcaaa
     3841 attttatgga aatacatttg ttatgattaa tttgttaatg tgcataacct gttcaagtct
     3901 tatcatatta actagaccgt tttcacatat tctttatgcg aaagactttt atgaagcgtg
     3961 gaaatatgtt ccatttttat taatagctaa tgttttaaat tgtgcatcag gtctgttagg
     4021 acctatctta tcagcaaaaa aagattctaa atccatgatg tggtctgcaa tcataggggc
     4081 attagttaat gtgattatga gtgtagtcct tgtttataag ataggggttc aaggtgccac
     4141 aatagccact gttatatgtt catatgtaat atatgttgtt cgtaaacgag ctgtgggaaa
     4201 agatattttt ataaagcatt atcatattgt tgtggaatct tggggattat tgtttgttca
     4261 ggcaatcata gaaatatatt ttaaaaatta tgtaatagaa attataatta tgattatttt
     4321 aattattata aatatcagtt atattaagca gttgtgttat agaggaagaa acattatttc
     4381 aatgattaag agtcgagagc acaaacggta gacgatgtta tttttataaa aagatataat
     4441 ttattaatgg tcgataatta tgaaaggaaa aatattctta aaaaaaagag tataggatgt
     4501 cgtatgaaaa tagtcgctat agtaccaatg aagttaaata acaaaagatt gccacaaaaa
     4561 aacacgaaag cttttaccaa tggaaaacca ttgtgttatt atatactttc aacactttta
     4621 aaagtaaaac agattgataa agtgtatgtc tattgtagta acccagatat taaagaattt
     4681 ataccagaag gtgtaacatt tttaaagaga tctagttcct tagatcagga tacaacgagt
     4741 attacacagg tattagccgg tttcacaaaa gaagtagagg ctgatgtata tgtaatgaca
     4801 cacacaacag caccatttat tagcgaaaaa tctattgaaa aaggactaga tgccgtgctg
     4861 acaggagaat atgattctgc atttgctgca aaaaaattgc aagatttctt atggaaagac
     4921 ggtgaaccat ttaattatga attggacaat attcctagaa cacaggattt gccgccttta
     4981 tacgaagaaa ctagtggatt ttatatttat aatcgcaatg tgatgaccga gttgcataga
     5041 agaattggga aaaaaccttg catagttgaa gtaggtgaga ttgagtcagt tgatattgat
     5101 gaagccgagg attttatgat tgctgatgcg atttataatc atcttatttt acaaggtagt
     5161 taagacaggg ggaaggttag gatgaacaat gtacagattt tagattgcac acttagagat
     5221 gggggctatt gtaacaattg ggaatttgga tttgataata caaaaaaaat tatttctgga
     5281 cttatagaat caggaattga gattatagaa tgtgggtttc tcacaaataa aacagtatac
     5341 aataaaaatg ttacaaaata tacgacctta gaagaattag cacagataat tcctgaaaaa
     5401 agagaaggaa aattatttgt agcaatgatg aactatggcg aatataatat agaggatctt
     5461 ccagattatg atggttcatc tattgatgga attaggattg cgtttcataa aaaaaattgc
     5521 aatgaagcgt tgaaattatg tcgtgagata aagaaaaaag gctacttagt ttttgttcaa
     5581 gctatggtat ctctcaatta tacagatgaa gagtttttag cagtaataaa aaatgtgaat
     5641 cagtttaaac catacgcttt ttatattgtt gatagttttg gaatgatgaa aggaaaagat
     5701 ttaactcgtc ttttttacat gattgagcat aatctagaaa aagatataca gataggtttt
     5761 cactcacata ataacatgca gcttgcatat tctaatgcac aaaaacttat aaccgtacaa
     5821 acaaatcgaa gcctgattat tgattcttcg gtgtacggaa tgggaagagg agcaggtaat
     5881 cttaacacag aactatttgt agaatatttg aatgataatg caggaaaaaa atatcaatta
     5941 aaaccattat tatatataat tgatgaaata ctaaatagtt tctatcagaa gagttattgg
     6001 ggatattcat taccaaacta tatttcagca gttcataatg ctcatcctaa ttatgcaagc
     6061 tatctgtcat ataagaatac tttaacaatt gaagcaatga atgaaatatt cgaaatgatg
     6121 gatgaagaaa aaaaggtatc ttatgataaa caatatatag aagatttata ctacaaatat
     6181 atggaaactg ggaaaataca ggaggaatat atggaagaac tgagaaaatt tctctcagac
     6241 aaaaaaattc tactgattgc accaggaaaa agttctgtag atgaggtaga aagaataaaa
     6301 gagtttgtta ccaaagaaga agtcgtaata attagtgtaa attttgaata ttctcatata
     6361 gatatagatt acgtattttt aagtaattta agacgttttc atgaattacc gaaagaaagc
     6421 agaactaaat gtattgttac atcaaatata caagcatcgg atgtatattt acggacgagt
     6481 tataaaaagt tgttatctga aagggaagtt gtaagagaca atgcaggatt aatggcaatt
     6541 agatttttag ccgatatgaa tgtggatgaa atattcttag ctggatttga tggatattca
     6601 cataatgaag atgagaatta tggtgagcaa tccatggaaa ttataacaaa atttgcatta
     6661 ttagatgcaa tgaacgtgca aatgagagat gaattgtcag aattagctaa aaaagtaaag
     6721 gtgacatttt taacggatcc acgttgggtt cgaattgata agtagtagta ataatgtttt
     6781 taaaattttg ataaagagat agtgagagaa taaaaatggg taaagtctta aataaaaaag
     6841 aagaaaaata tttaagggcc gatggggcga gatgaaactc taattttgaa cttttaagaa
     6901 tcatttctat attgtgtatt gttgcttacc atgctaattt gcgtttaaat tataatgcag
     6961 acggaattat agtacatggt tcattaactg ctaaccagat tttcttgaat tatattggta
     7021 tttggggtca gctaggagtt aattgtttta tatttatttc ggcatatttt ttaagtgaga
     7081 aaagaaacat tcactttgaa aatgttttgg gaattgtatt agaatgtatt ttttatagtg
     7141 tattagttta tggaattctt tgtgcatgtg gaatagaaac attttcattg aacagctttg
     7201 taaaagaaat attatcattt gctctaggta gatattggtt tattacatcc tatttagcat
     7261 tgtatattct aagtccgtat ttaaatatca tgcttaacaa tctggaaaaa cttcagttgc
     7321 aaaaaataag tatgttattg tttataggtg tatgctgtta tcgtatgtta tttaaaacat
     7381 ctttgtttgg ggattgtgct gttttaattg cactatatgt catatgttat acactgaaaa
     7441 aaacgataat aatatttttg agagacacag aatcatagga acaattgtat gtataacagt
     7501 tataccactt ctcatatcca taggaatact tttcaaattt ataccaaatg caatggttaa
     7561 ccagatggtt catattggtt ctccattgat ggttatagca agtatatttt tattttatgt
     7621 atttaaaaat ttttcatttt acagtaagta tataaataaa cttgcgagaa ccagtttagg
     7681 ggtatatatt ttacatgaaa atcattattt cattgatggt atgaagtcta gaggcttaat
     7741 atgggatggg atatttggat taggcaaaag gtttttagat agggattttt ggatttggtt
     7801 tataattgca gttgtagttg tgtgcataat ttgcatggct atcgatgttg taagagaaaa
     7861 atttgaaaga cagattttta aaattaagag tgtaaagaac ctgtgtaaac ttatgaatca
     7921 tgttataaat atatacccat aattaataaa tgaggagaaa agaagtgaaa gttatatcat
     7981 tcggagaatt attattgaga cttgcagcac ccggttattc aaagttattt caaagagata
     8041 gtttggagac tacattctgc ggaggggaag ctaatgtagc ggtatcctta gcaatatttg
     8101 gatatcaatc agaatttata acaaagcttc cggattcaga tgttggatat gcggcagcta
     8161 atgctttgcg ttattttgga gtaaatgttg ataatattat atttggagag ggacgcatgg
     8221 gactgtatta tttagaaaaa ggagcagctc agagaccatc taaagtaatt tatgacagaa
     8281 aattttctgc aatttcgtta gctgatagtg atgaatttaa gtggaaggag atcctaaacg
     8341 atgcaaattg gtttcattgg acagggataa atccagcatt gtctgagaat ctctttgaga
     8401 cgtccttaga tgcatgtcag gctgccaaaa aaagaggtat aacaatttct tgtgatttaa
     8461 attatcgatc caagctttgg acatcagagg aagcaaaaga aaagatggaa cagatactac
     8521 cattggttga tatttgtatt tgtaatgaag aagatgcaga aaaagtgcta ggaattaaag
     8581 ctgagcatac aaatattaat agtggagaat taaataaaga tggctatgtg caggtggcta
     8641 gggagattca caagaagttt ggatgtaggt atgtagctac aactctgcgt agaagttatt
     8701 cggcaagtag aaatggttgg agtgcaatgt tatacgaagc agaaaaagat aaagcgtatt
     8761 tttccacaga atacaacata caaattgttg atcgcgttgg tggtggagac agcttttctg
     8821 ctggtataat atatgcgtta tgtgaaaata tgtccggaca aaaggctatt gaatttgcga
     8881 ctgcatctag ttgtttaaaa catactatag aaggtgattt taatcgaact acaattgagg
     8941 atgtatataa tttaatgaat agcggtggaa atggtcgcgt gttgcgttag tcaacatata
     9001 aagataaatc acagtaattt ttatggtgat tatctgtctt agtagtgatt agaataacgt
     9061 gaaaataaat tgttgagcgt ttatactgtt ttgaaaaata aatttaacat attgaaaaat
     9121 acaaatatat acgtatatta taaagcgccg attgtaaaat taagctagac aactaaaaca
     9181 gtcatttgtg ctacactcaa aatagttccg accaaagaat tataaggagt gagtgcaaat
     9241 aacctatacc tatcttacaa cggatgaact tgtaataata gagtcttatt tgaaaattaa
     9301 ccaatctgtt gctaaaactg cgcattgctt gaatcgttca agtcaaacga tccataaagt
     9361 atatctgttc ttcaagcaaa gaaaatcagc cttagaata
//
LOCUS       DAYYAO010000101         9286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000101 DAYYAO010000000
VERSION     DAYYAO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.105"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACHARI_002425"
     CDS             <1..227
                     /locus_tag="ACHARI_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443433.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACHARI_002426"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACHARI_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACHARI_002427"
     CDS             1635..1841
                     /locus_tag="ACHARI_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443434.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACHARI_002428"
     CDS             1862..2458
                     /locus_tag="ACHARI_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFM8443435.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="ACHARI_002429"
     CDS             3410..4009
                     /locus_tag="ACHARI_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443436.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="ACHARI_002430"
     CDS             4058..4774
                     /locus_tag="ACHARI_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFM8443437.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="ACHARI_002431"
     CDS             4788..5567
                     /locus_tag="ACHARI_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM8443438.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="ACHARI_002432"
     CDS             5650..6006
                     /locus_tag="ACHARI_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8443439.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="ACHARI_002433"
     CDS             5996..6262
                     /locus_tag="ACHARI_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8443440.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="ACHARI_002434"
     CDS             6421..6699
                     /locus_tag="ACHARI_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443441.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..7377)
                     /locus_tag="ACHARI_002435"
     CDS             complement(join(6696..7343,7345..7377))
                     /locus_tag="ACHARI_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8443442.1"
                     /translation="MNHFKGKQFKKDVIIVAVSYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLDYLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFSVCTEI
                     RILLGIPA"
     gene            complement(7409..7570)
                     /locus_tag="ACHARI_002436"
     CDS             complement(7409..7570)
                     /locus_tag="ACHARI_002436"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFM8443443.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(7894..8226)
                     /locus_tag="ACHARI_002437"
     CDS             complement(7894..8226)
                     /locus_tag="ACHARI_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443444.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            complement(8390..9007)
                     /locus_tag="ACHARI_002438"
     CDS             complement(8390..9007)
                     /locus_tag="ACHARI_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443445.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            complement(9090..>9286)
                     /locus_tag="ACHARI_002439"
     CDS             complement(9090..>9286)
                     /locus_tag="ACHARI_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8443446.1"
                     /translation="NNLIEQDHRPIKRRNKFYRSLRTASSTIKGMETIRGIYKKNRRN
                     GTLFGFSVSTEIKVLMGIPA"
BASE COUNT         3272 a         1445 c         1661 g         2908 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatga tcttgttcta ttaaattgtt aagatacttc
     6901 acagttcggt gctctgtctt agtatataaa cccacactct gtaactttct aaaggcggag
     6961 ccaagagaag gtgctttatc ggtcacaatt gctttcggct caccaaactg tttatggagt
     7021 cgttttaaga aagcataggc tgcttgcgta tcccgtttct ttcgtaacca gatatctaag
     7081 gttaagccgt ccgcatcaat tgcacgataa agataatgcc aatgtccctt aattttgata
     7141 taggtttcgt ccattttcca tgaatagaag gattgtctat ttttcttctt ccaaagataa
     7201 tcgaggactt tgctgtactc ttgcacccaa cgataaatcg tagtatgaca aacatttatt
     7261 ccacgatcat ataacaattc ctgaacttca cgatagctta gattgtaacg caggtagtaa
     7321 ctaacagcga caataatgac gtcatttttt gaattgtttg cctttaaaat gattcattac
     7381 tctatcctct ctgtcttttt tctcaatttt acactaaaat agattttttg gaaaactttg
     7441 caacagaacc acttttcgta atatcaatgt ctttattcgt atgatttttc gttttacgat
     7501 ctacatgatt ttgaagtcca tttaacgacc caactttatg cttttgtgtt cgtgccacca
     7561 tataggccat aactgtgtac ttcctttcgt taaaattatt gcgagaatca caagctaaat
     7621 tcggaaaaac ctccatgtaa ggtataactt actagactta tactgcgtaa agtctgtaag
     7681 ctctgccgag ggctatatcg gcttaacagc cgatgagaaa ctccgtttct ctcaaacgaa
     7741 aaagcgaacg aaaaacgact gccttcgcat tcctttttta cgtccttttt tcgttcaaaa
     7801 ccattggctt tttgcgtcat tgttttcaca atttttattc cgcaaaaaac caaaagtgaa
     7861 aagtattgct tttcacttca acccctcctc aatttagttc acattttctg aacgaaattg
     7921 tgccataaat tgagtcaaac ccttaacatc attcgtacgc caatatgcac cattttcttg
     7981 ccaaactaat ccatctgcaa attttcttag cgatactaaa acctcatcat ttactgaatg
     8041 atttctttta tttgattcca aaaaatctaa tgcttcagca ggtgtcttaa agccttcctt
     8101 tttacaatat aatttcgcta aagaaatcat ttctttctgt ctaatctgtt ttcgtttcgc
     8161 tctaatcttc gcttcttttg ctttcaaatc cgccatttct tttgccaata tttctctatt
     8221 gttcattttg attcctccta aaaataaggc atatttctag tttaaaatat ttattttacg
     8281 aatagaggat ttgaacaacg aatgacacaa aaacaacaaa aaaagagcaa tcatcataag
     8341 atgattgctc taaaaaactg tagtattttt ggccaaattt ttaatttttt caatccaaat
     8401 taaaaatttg ggatactcta tttttcaaaa aacttctaaa ggtttgctca aattttaaaa
     8461 tagtacgtct atcaccagta gaaccatagc gcatatttgc aacaagatca taagtgcata
     8521 aacgaccatt catttttaat ttaactttga aaacaggcat tgaagtacct gaatttgttt
     8581 ctaaaaaaaa ggctgcatat tcatctggtt ctaatgaaat tttgttttga cctcttattt
     8641 gtacctgttc aaacatctgt ttttcaacgt tttttgcaga aaaatcttca atgactggag
     8701 gattttcaag cattttcaac aatttaatct ccaatggttt ttcttcgcaa ccaaataccc
     8761 aataataaga tggagaagta ctgttaattt catgactatc gtacaattca actctatgtt
     8821 cctttgaaaa atcatcgaat tcatttcgat tcattttttt cactattact ttctcataag
     8881 aatttttttt atcaacaaac cagatccaca tagatcctaa aaataaaaaa ataaaaaata
     8941 agattcccca acctaatcca tctaaactaa ataatttagc aaagatttct tgtttaaata
     9001 tccacaaaac aattttcctc ccactttaaa ataatggttc tgttgcgaag tttgaaaatc
     9061 aaacgcgccc tctctgaact ccaaatatct taggctggta ttcccattaa taccttgatc
     9121 tcagtagaca ccgaaaagcc gaagagcgtt ccatttcttc ggttcttttt atatattcct
     9181 cgaatggtct ccatcccctt aatcgtggat gaggcagtcc ggagacttcg ataaaattta
     9241 ttccgtcgct taataggtcg atggtcttgt tctattaaat tgttca
//
LOCUS       DAYYAO010000102         9012 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000102 DAYYAO010000000
VERSION     DAYYAO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.106"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..947
                     /gene="purR"
                     /locus_tag="ACHARI_002440"
     CDS             129..947
                     /gene="purR"
                     /locus_tag="ACHARI_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8443447.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1450..2823
                     /gene="glmU"
                     /locus_tag="ACHARI_002441"
     CDS             1450..2823
                     /gene="glmU"
                     /locus_tag="ACHARI_002441"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8443448.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2917..3888
                     /locus_tag="ACHARI_002442"
     CDS             2917..3888
                     /locus_tag="ACHARI_002442"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8443449.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3894..3996
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4062..4631
                     /gene="thiT"
                     /locus_tag="ACHARI_002443"
     CDS             4062..4631
                     /gene="thiT"
                     /locus_tag="ACHARI_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8443450.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4712..5485)
                     /locus_tag="ACHARI_002444"
     CDS             complement(4712..5485)
                     /locus_tag="ACHARI_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443451.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5752..7452
                     /locus_tag="ACHARI_002445"
     CDS             5752..7452
                     /locus_tag="ACHARI_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8443452.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7422..7640
                     /locus_tag="ACHARI_002446"
     CDS             7422..7640
                     /locus_tag="ACHARI_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8443453.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7660..8550
                     /locus_tag="ACHARI_002447"
     CDS             7660..8550
                     /locus_tag="ACHARI_002447"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8443454.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8568..8852
                     /locus_tag="ACHARI_002448"
     CDS             8568..8852
                     /locus_tag="ACHARI_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443455.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3038 a         1539 c         1890 g         2545 t
ORIGIN      
        1 tttataggct tctttttatt ttaataaata ccgaactatt ttcttatttt ttcataaaat
       61 gctcgaattt ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt
      121 tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga
      181 atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat
      241 cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa
      301 ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg
      361 aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc
      421 ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag
      481 ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag
      541 ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta
      601 ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg
      661 gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac
      721 gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct
      781 tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca
      841 acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc
      901 aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa
      961 agctgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca
     1021 taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa
     1081 atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc
     1141 cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta
     1201 agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt
     1261 atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata
     1321 ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact
     1381 tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga
     1441 gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg
     1501 aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc
     1561 attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca
     1621 gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg
     1681 ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg
     1741 ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa
     1801 tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca
     1861 ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa
     1921 gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac
     1981 aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac
     2041 tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa
     2101 acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat
     2161 gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat
     2221 tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca
     2281 ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca
     2341 aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc
     2401 agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa
     2461 attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa
     2521 ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat
     2581 gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc
     2641 ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa
     2701 aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca
     2761 attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac
     2821 taagactttt gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag
     2881 aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca
     2941 agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca
     3001 gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta
     3061 aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca
     3121 gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca
     3181 aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt
     3241 gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca
     3301 agaatgctga ctttggacct tcatgcagtt caaatccaag gtttctttga tattccagtc
     3361 gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc
     3421 gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg
     3481 gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca
     3541 gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg
     3601 atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact
     3661 agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa
     3721 gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa
     3781 atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc
     3841 catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca
     3901 caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc
     3961 ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt
     4021 cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt
     4081 gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga
     4141 tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa
     4201 gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg
     4261 gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt
     4321 ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata
     4381 cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt
     4441 tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc
     4501 cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga
     4561 tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac
     4621 aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg
     4681 tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg
     4741 catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt
     4801 cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg
     4861 ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct
     4921 gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt
     4981 ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt aatttactag
     5041 caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa
     5101 gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt
     5161 tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg
     5221 ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg
     5281 aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat
     5341 attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac
     5401 tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa
     5461 tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt
     5521 attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa
     5581 tcacgttttc attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt
     5641 tatttaaagg aggttttatg aattgttttc ttatggaatc tacaaagtcg aatacgagac
     5701 tcatgacctt tttatgacgt actggtatac ttgaagaaaa aggagagcgc gatggagata
     5761 aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc ctatccagca agcaaaacaa
     5821 ccccttccat tttttcaaag tcatcaagaa agtagagacg ctttttggcg ttctgcaaaa
     5881 aagcaatgtg cttttaaagc agtatcgaaa aaatataccg tctattttac tattgctaaa
     5941 agtagaggag atgttatttt taataatctg ttgattgaag gtgaaacttt tgaatggaaa
     6001 aaattttttc ggtatatgga agcttgcgct cgttttttta ttaaagaaaa ctgttgtttt
     6061 ttatttcaat ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa cggctatcaa
     6121 ggaaccacac aattaaataa aaagctggtc tatcatacag cacttgtttt aggtggagga
     6181 ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca tgatatcaac
     6241 tttgagatta tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat tttacaagga
     6301 gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga caagacaagt gcttgcgtta
     6361 ccagaaatgg ctgcagttga agaccttcgt gaacgcttct atcgagaaag acgccaaatg
     6421 acgaaaacag cactaattga aggcggggtt tcttctgctc cattggaaaa gatggtcaaa
     6481 gacaatttgg atttgtcctt actcaaacat aatcccaaaa tacgtttata tacggtgtct
     6541 actaaactgc cagaactagc ggaagtagtg actgatatcc aacagacaaa aacagaagaa
     6601 ataccagatt ggatactcgc ctctgcttca ttttatcccg ctatggctta tcgaaagatc
     6661 ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa tacctattga tattgcgatt
     6721 aacaaaggag caacagaggc atttgtcgta gatgtgcaag ggccaggccc agcgaaaaaa
     6781 attcgtatgc cagacatttt tatccattgg aaatgtcaaa cattgtggac actaggcagc
     6841 tttctgcttt ttgatagcca aaggaaccaa ttaaatttac aattaggcta tctggaaatg
     6901 aaaaaacgat taggctatta ctatgggaac tggtatacat ttcattctgt taaacctgca
     6961 agaacatact ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt agaattatct
     7021 tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa cagggtcgta
     7081 ccagagacat gcggattggc gatgctagaa cttttagcaa aaaaacattt tttactgccc
     7141 aatgaaatct atcaagtaga catgatgatt caaatgatct gccaacaaga tataaaaagt
     7201 atgccggatg atttattggc acaaattggt caattaagta ctgaagaatg gcgaaggtat
     7261 cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt tgattatctg
     7321 ctacaaggaa aacgcaagga tcgtttgcag catgaattgc ttaagcaacc agttgacaca
     7381 ttactgatcc tatatttata ttatctcaag gaggaacaac catggcacaa gaattttcat
     7441 atgaaatcgt agaagaaatc gctatattgt cagaaaacaa caaggggtgg cgtaaagaac
     7501 tgaacctagt cagctggaat ggtcgcccgc caaaattcga tcttcgagat tgggcgccag
     7561 accatgaaaa aatggggaaa ggcctgacat tgacgaacga agagtttgaa caactgcaaa
     7621 aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat gactggtttt
     7681 ggcaaagcca tccgcgaaac aaaagcgtat cagttagata ttgaaataaa aagtgtcaac
     7741 cagcgttttc tagatgttca aatacgaagt cctaaaatcc tgaactttgt tgaaaacgat
     7801 tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt tgtgaatcta
     7861 gcttatattg gcaaaaacga aaaacagatc gtcatcaact gggaattgat tgattcactt
     7921 atcactcaat tgacagacgg aattcaacaa cgatacggta gtcaaaatca agtgtcaatt
     7981 agtcggatgg cggaacgctt cgcgctggac gaaaacttcg taacggtaga agaaaaaccg
     8041 gaagagagca tggaagaatt ggcacagtta gctctagata cattgaaaag cgcacttgat
     8101 tctattgagg cgagtcgaga aaaagaaggt caagctttag ctttagtgtt gaaaaagaat
     8161 actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat ctatgaaaag
     8221 gaatttcaaa taaggtatca aaaaaagctg gaggaatttc ttggagcgaa agtcgatgag
     8281 caacggttgt tgacggagct agccatcttg ttggaacgcg gagatattca cgaggaactg
     8341 gatcgtctgg cgatccatgt acaaaaacta gatgaactta tcgaagcaca atgtcctgta
     8401 ggtcgggaat tagatttcct gatccaagaa atgaatcggg aaatcaacac gatcggttcc
     8461 aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat cttagaaaaa
     8521 atcagagaac aagtacaaaa tattgaatga cgaaagaggt agagataatg aaatattatg
     8581 acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa acgggatatc ccttctgaaa
     8641 aaaacggctt tgacgtatgg aaagatgcct gtgcagacta taatgagaac gaactgttta
     8701 ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa atttattgtt cgaattgaca
     8761 cagaagaggt agaagatccg acagaaaaag cacgttctcg aaaagatgag atcatgggtg
     8821 tagtgaatac tttgtccaat atgggctttt agcaaggatg ctgaacaaag aaatacaata
     8881 aataatcaga attggatttc ttcgcttagc ttctttgctc taggaataca tttaaaggtt
     8941 gtgacaaaat tcgtgtcaca atcttttttt attttgaata agtgatgttc aaaaaccatt
     9001 ttctcggtat ta
//
LOCUS       DAYYAO010000103         8867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000103 DAYYAO010000000
VERSION     DAYYAO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.107"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..1379
                     /locus_tag="ACHARI_002449"
     CDS             39..1379
                     /locus_tag="ACHARI_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8443456.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1597..2040
                     /locus_tag="ACHARI_002450"
     CDS             1597..2040
                     /locus_tag="ACHARI_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8443457.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2544..3962)
                     /gene="nhaC"
                     /locus_tag="ACHARI_002451"
     CDS             complement(2544..3962)
                     /gene="nhaC"
                     /locus_tag="ACHARI_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8443458.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4365..5588)
                     /locus_tag="ACHARI_002452"
     CDS             complement(4365..5588)
                     /locus_tag="ACHARI_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8443459.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5619..6458)
                     /locus_tag="ACHARI_002453"
     CDS             complement(5619..6458)
                     /locus_tag="ACHARI_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8443460.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6475..7155)
                     /locus_tag="ACHARI_002454"
     CDS             complement(6475..7155)
                     /locus_tag="ACHARI_002454"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8443461.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7157..8230)
                     /locus_tag="ACHARI_002455"
     CDS             complement(7157..8230)
                     /locus_tag="ACHARI_002455"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8443462.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8286..8524)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8567..8800)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2543 a         1814 c         1616 g         2894 t
ORIGIN      
        1 taaattttga ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa
       61 aacaacttag ctccagacat atcaccatgt tagctttagg tggagcaatc ggtgcaggtc
      121 tatttaaagg aagtggcgaa gcgattggga tcgccggccc ttctgtattg attgcttttt
      181 tgattggtgg gctgatttta tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg
      241 gtggcgacac gcatcacgga ttatctggtt tgatacgccc ttatctaggt gctcattctg
      301 ctgattttac tgattgggtc tactggtcta tgtggattat caatatcatc gctgaagctg
      361 ttgcagctgc ttcctttttg cagttgtggt tcccgcacat tccagcctgg gtatttgttt
      421 ttatgttggc tgttttaact acgatcatca atctttattc ggtacggtta ttcgcagaaa
      481 cagaatattg gctggctttt gctaaaatca gtgtcattat cttactgatc atttttgcta
      541 cttatcttgt cggtcagcaa atgttgggca caggcgtttt ccctaccctt cagcaattga
      601 ctgaccatgg tgggtttact cctcatggta tgaagggaat catcagttct ttgcttgtag
      661 ttatctattc ttacggcggt tctgaactga ttgctatcac ggtaagcgaa gcagatgatc
      721 ctaaacgtgc gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt tccttttaca
      781 tcattccttt gtttttgctt ttgattcttt ttccttggaa tactttagca ggcacgaatg
      841 tcagtccttt tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca
      901 attttgtgat tgtccttgct ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa
      961 gaattttgtt cttccgtttg aaggatcgaa aaggttcctc tagaaaacta gctgttttaa
     1021 acaagcatca agtaccgcaa cgtgctgttt tattttgtac cagtgttctt tatcttggtg
     1081 tagctctttc ttattttgtc ggtgacaaat tatttggcta tctggcaggt tccctttcgt
     1141 atacagtatt attgatctgg atcattatca gtgcggcatc ctttgtactg gcactaagaa
     1201 aaggaacatt atgggataaa ggaatcagct tttttgcatt agctgtctta ggattgattt
     1261 tccttggtat cctgttcacg aactccattg gggtaactgt attgactggc ctgctttacc
     1321 tgttcattta tttcagttat caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa
     1381 atcgatagaa aattttattt tatttatgtg gaatagatgg agctattgaa gaaaggagtt
     1441 gtgacaaaag tcttgttgca actccttttt ccgaataaac agtctcatga cctcttggta
     1501 aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt tctccatgct catgacatga
     1561 tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg
     1621 ttgatgatcg gtgtttcaac ggtagttatg tttggtatga tgtacttgaa tgtctatgcg
     1681 ctcgatcata tcttcttcag tcgtacacga gtattcatgg cgttgatgat gggtgcagtc
     1741 atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt
     1801 atgatcttag ccgctagtct tcttgttttt gccggttcct tgtttatggt acgaagccaa
     1861 actactgtca acgacacagc ttggatgaag gccatgattc ctcatcattc tatcgctatc
     1921 ctggcaagta aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc
     1981 attcaggcac aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga
     2041 ccattatccg tccaaataaa aaaagaggat gttccagaag ttatcagctt caagaaataa
     2101 gaagaagcta cttctggaac gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa
     2161 gtaataagaa cagccaaaca aaaatagctt ctcctatttt tcgtttggtt aggcttatta
     2221 ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtaaaatcag cgttctgacc
     2281 tgagtattag agagacaaat ggaaaaatag ttcctcatat tttgccattt tttgagctaa
     2341 taccgagggt cagctgattg aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt
     2401 tcgcgtatta agaaaaattt tgtgaaatgg ctttccacat ttttgtataa atttggctta
     2461 ataccgagaa actggctttt gaataccgtt tattcgtaaa agggattgtg acaaaagtct
     2521 tgtcacaatc cctttttatg tttttattgt tctaattgcg ctgtcttgct tactgcttct
     2581 tgcggtttca caaatttttt tgagattccc caacctgtga aggcactgac catcgaaaac
     2641 acaggagaaa gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg aataccaaat
     2701 gtacttgcta cgaaacttcc ggcaattccc caaggcacta agtagttgat tactgttcca
     2761 ccatcttcta gcgttctacc taatacagtt ggatcaaatc ctgctttttc ataagtttct
     2821 ttaaatgcat taccaggcag aataacagat aggaattgtt caccaataaa aatatttact
     2881 ccgatacttg tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat
     2941 actcgttcca tcacggttga gatcaagcca aattcgatca acaatccacc taatgacaaa
     3001 gttagaataa tcaatgacac agtaggcatc atactttcga ttcctccgcg gctaagcagt
     3061 gaatcgattt gttggtttcc agtatttgaa acaaaccctg attcgatgac cccagccaac
     3121 ttcgttaagg aagtttgtgg gttttgaata aaaatcattc ctactgctac agcgatattc
     3181 aacaaaatcg ttaaaattgc cggcaccttt ttccatgcac acagtaacat taaaaataaa
     3241 ggaatcagtg accagattga aatagtaaag tttgcttcta gaacatcagt cacttcacga
     3301 atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa
     3361 acaacaaatg ccgggacagt tgaccacatc atatgcttga tgtgtttgaa caaatcggca
     3421 tcaacaatag ctgctgctag atttgttgat tcggataatg gagacatctt atctccaaaa
     3481 acggcacctg aaacaatagc tccaacaacc agcgctggat ttaagcctag tgttgtccca
     3541 ataccgaaaa atgcaatccc aatcgttgac ataacagtaa aggcactccc tacagcgcta
     3601 cctacgattg cacacacaac aaatacagaa gggacaaacc actggacact gatcaatttg
     3661 aacccaatga ccattagagt tggaatgatc cctgcttgta tccatacagc aatcaatgaa
     3721 ccgactaaaa taaaaataaa gattgggatg attcctggtt tgatgccatt gatgattcct
     3781 tcgtgcagcc aatctgtcgg gtatcttttt atcactgcat acaacatgac cattccgatc
     3841 gctgctaaga tcgtgatatt aggtgagacc tttaatccga caacgccaat cgcaatgcct
     3901 aaaacaataa ccattaatac aagtgttgct tctaaaaaac ttggtgttcc tttttttccc
     3961 atgataatct ctcctttttt agttgttctt atgttctaaa aaagttaaga tatcggatta
     4021 ctccgccgca attcatccgc gattcctcct ttataaaaat aaaaagacct gttttaaact
     4081 aaggacgaat gactccgcgg taccacctta attaaaaaca gatctgtttt tcactttcaa
     4141 acttttattc aaatgtatgg actttccatg taattcgtca attaggcttg atcggttcac
     4201 agcgaccacc gactttctga acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt
     4261 acgaagttta ccttccataa gatcatttgt caatgaaaaa gaaaatattt tttcattttt
     4321 ctgaaaatta tttttataac tttcacacac aagattattt tttactagaa agtagcgccc
     4381 tcttgttttt ttcttgctac tatttcttga tgttcttgct tgattctttc taatagcttt
     4441 ggctctttca acagatcaag agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa
     4501 gcaaggcctt tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat
     4561 gtctcagaga ttgctaccgt aggctggatt gttggtatga cttggctgac atttcctaca
     4621 tcagaagaac cgagacttgt tctttgttcc gtttctattt cttcatctgg aacacctgct
     4681 tcttttgcat gctggaagaa taagtcatca aaagaaggag taacgatgac atcatcaact
     4741 gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg
     4801 atattttcca ctttttcata tactttatct aatgtttgcc gatttcctgc acgtaaatag
     4861 aaacgtccag cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc
     4921 ccatggatat tgacatcttt tggcaattgc aagcgtagag cgttgattcc attaaacact
     4981 tggatcaatg cttccaaagc atttactcct ttttcaggtg ctgctgctgc atgggaagcg
     5041 acaccgtaaa atttgatgtc gactggatca ttcgctaaag attctgtcgt tttcccataa
     5101 cgaaaagctg gatggacgca taacgctaca tcgacatcct caaaaaaacc ttctcgaaca
     5161 aagctgcctt tagctgatcc gttctcgccc ccttcttccc ctggggtgcc atatacacgg
     5221 atttctccac cagtttcatc aatgaattgc ttcactatgc tggcggctaa tacggagtaa
     5281 gtaccaaaca aattatgccc gcatgcatga ccaatacctg gtaaagcatc gaattctgcc
     5341 aaaaaggcaa ttgtgggacc gggtttattg gaaatataac gtgcatcaaa ggctgttcgg
     5401 tgtccagcta cttctttttc tacccaaaac ctttcttttt ctaattgttt aatcaacgct
     5461 gctgaggaat aaaactcata gttacttact tcaggatgtt cgtggatatc tagagcaagt
     5521 gcttgatatt ctggtaaacg atattggatt agatccttca attggtttgt tgagattgtt
     5581 gttgtcataa aaaattcctc ctatgctagt catttttctt attttctccc gaatgtttcc
     5641 catgcaggca cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt
     5701 tgataagcgt ctactatttt ttgatacgtt ttattgtttt catcttcttt tcttgcaacg
     5761 atgatattca cataaggacg tgcatggtca tccactggtt ctaagaagat ggcatcagaa
     5821 gcaggattaa atccagcatc tacagctaca ccgctattga tcactgaaat gtctacatct
     5881 gacaaagcac gtgctgtttg ggaagcatct aattcagaaa tgtctagatt ttttctattc
     5941 tcggtaatat cagaaacagt gggcgcttgt ccttttgcct tatccacttt gatcaatcca
     6001 gcagtttgca ataacagaag agctctgccg ccatttgtaa catcattagg gattgctact
     6061 tttgctccgt ctttgatatc agctacatcc ttcaaagtgt tagaataaat tcccagcggt
     6121 gcattgacag tatttccgat cgatactaaa tcagttccgt gttctttatt gtaattgtct
     6181 aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc catctgctaa agccacattt
     6241 ggttgattgt aatctgtaaa tttcacgtat tccaaattaa tgttctcctt tttcaaacgg
     6301 tctttcacgt tgtcccatac atctgtatct tctccaataa tccctagttt tacgatagtt
     6361 tcttttttgt cagttgtatc tgcttttcct tggctacacc ctgccaacca aataattgct
     6421 acaaatgtaa tgactacaaa acgaataatt tttttcattt tttctcctcc actttcaatg
     6481 tgtaattttt ttaatcagtt gattactgat aaattgactt aaaaaaacta agattagaat
     6541 caataatgta gccacaatcg ttacatctgt ttggaatcga ttataccctc tcgatatagc
     6601 aagattccct aatccgcctg cacctactgc tccagccatg gtagtcagtc cgatcacgtt
     6661 gatgatagtc aacgccgaga cccgaatgat accaggcaaa ccttctaaca aatacacacg
     6721 taaaatgata ccgattggac tggttcccat cgcttctgcc gcttcgatca ccccaggatc
     6781 aacttctagc agtgcgttct cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa
     6841 tggcactaaa gctgctttcg caccaatcgt cgttccaact aagaaacgag tagtcaatgc
     6901 taaaagagaa agcaagataa tgaaagggat ggaacgaatg atattgatca gcttatcaag
     6961 tatttcaaat agcagacggt tagctagtaa tccattaggg cgtgtcacta ctaacgtcac
     7021 acctaagatg attccaatca tcccgccaat gaatgctgtc caaaaagaca tatatagtgt
     7081 ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa acattgggca aatatgtttt
     7141 aaagaaatca gtcattttat cttcctcctt cgatcacttt gatacgacta tctaaatcgt
     7201 ttttttgtaa aatatttatt tttacaccag atttgatcaa ataatccaat gcttttttcc
     7261 gctgtttggt ttctccagct aaagtaacga tcaaactccc taacggcgtt tcttgtaata
     7321 actctacatt gccatataaa atattcgctg ttacttgata tttgctgtac agatgagcga
     7381 ttagaggttc gttcgtctga tttccaatat aacttaattc caccagccat ttattggcga
     7441 tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca agatgagtag
     7501 ctgttcgaat aaaatctttg gtcaattctt cttcaggatt gctgaagatc tggatgctgc
     7561 tacctttttc aacgattctt ccatcttcca taaccgctac cttgttgcag atttctttga
     7621 caacttgcat ctcatgagtg atcaggacga ttgttaaacc taactggcga ttcaattttt
     7681 ttaacaatgc aaggatctga agggtcgttt tcggatcaag tgcactggtt gcttcatcac
     7741 ataataatac cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc
     7801 cccccgacag ttgtttagga taagcagatg ctttttcttc caaaccgaca agttccagta
     7861 gttcattcac tttttgacga cgttcctgtt tcgattttcc agaatacttt aaagaaaaat
     7921 caacgttgtc aaaaatagtt cggctttcca ttagattgaa atgctgaaag atcataccga
     7981 tggcctttct ttcttttcgc aattctttgg gagagagctg actcagattc gtctgattga
     8041 caatgacttc tcctgctgtt ggtttctgca gcaaattcat cacacgaact aacgtacttt
     8101 tccctgcacc tgagtaaccg acgattccat aaacatcgcc tttttcaatc gttaaatcta
     8161 cagaatctac tgcctgtatc tctttttctt tttgtttgaa acatacagaa acatctcgca
     8221 attcaatcat taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc
     8281 attttcttga ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac
     8341 aactcctctt caagtacgat catactctag caagatatcg gtcgcagccg tcatcgcctt
     8401 acatctatgc tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt ccttgcttct
     8461 tctcagctac cgaagctctc tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa
     8521 ataatattca aaaaaaaagt ccttttacta acataaacta tgtcagtaaa aggacgacgt
     8581 atcgcggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag
     8641 agattggcgt tcgttttaac ggtcgaacag ttccgaattt gcctacttat cgacaaatta
     8701 ttctccaagg ccattttcag cttttcttac ttgactcttc tcaccagcca gagcctctct
     8761 gcaaagtttc caaacttact ttccttttca acgaaatcga tattttgttg ttgggattaa
     8821 tctacatgag gaaaactgaa aagtcaagta cgaaattcaa attattt
//
LOCUS       DAYYAO010000104         8591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000104 DAYYAO010000000
VERSION     DAYYAO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.108"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..613
                     /locus_tag="ACHARI_002456"
     CDS             <1..613
                     /locus_tag="ACHARI_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8443463.1"
                     /translation="GEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEE
                     EFGHLEGDTIVGIHHKSAIITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNL
                     FKSITFDCGKEFSNWKSISNTNDIDIYFANPGTPSQRGLNEHSNGLLRKNGLPKEMDF
                     NQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(681..1454)
                     /locus_tag="ACHARI_002457"
                     /pseudo
     CDS             complement(681..1454)
                     /locus_tag="ACHARI_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1490..2677
                     /locus_tag="ACHARI_002458"
     CDS             1490..2677
                     /locus_tag="ACHARI_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFM8443464.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            3410..4378
                     /locus_tag="ACHARI_002459"
     CDS             3410..4378
                     /locus_tag="ACHARI_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8443465.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            4389..5204
                     /locus_tag="ACHARI_002460"
     CDS             4389..5204
                     /locus_tag="ACHARI_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM8443466.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            5272..5982
                     /locus_tag="ACHARI_002461"
     CDS             5272..5982
                     /locus_tag="ACHARI_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8443467.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            6018..7259
                     /locus_tag="ACHARI_002462"
     CDS             6018..7259
                     /locus_tag="ACHARI_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8443468.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNESSPQSCVKVQPHQKH
                     NVLRCEPNCHAFLSEERGQEQ"
     gene            complement(7317..8504)
                     /locus_tag="ACHARI_002463"
     CDS             complement(7317..8504)
                     /locus_tag="ACHARI_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8443469.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         2852 a         1429 c         1693 g         2617 t
ORIGIN      
        1 gggagaattc aatgttttac atttaccgat gcaaggaaaa cgaaaaccaa atggttataa
       61 ggaaaaacgt ggaaaacagg cattcaaaag aaatatttct gaacgtaaaa aagattatgt
      121 cgttttcgaa gaagaatttg gacatttaga gggtgatacg attgtcggca tccaccataa
      181 aagtgccatc atcacactcg ttgagcgact ttcaaaagcc attatcgcct tgaaaccaga
      241 aggccgtaag gcagttgata ttgaaaattc gattaatgaa tggcttcaat ccgtacccaa
      301 aaatcttttc aaatcaatta cctttgattg tggaaaagaa ttctctaatt ggaaaagtat
      361 tagtaatacg aatgatattg atatttattt cgcaaaccca ggaacgcctt cccaacgggg
      421 attaaatgaa cattcaaacg gactcctccg aaaaaatgga ctaccaaaag aaatggactt
      481 caaccaagtc aatcaaggat tcatctcatc cgttgcgtcc aaaagaaacc atatccctag
      541 aaaatcacta aattaccaaa caccattaga agtttttttg agttacgtaa atggaaagtt
      601 ttgtctcgct taatttgaca aataataagc caataaaact tagtccgata tgcggcgcgt
      661 tctgtgcgcc gcatattttt ttagttttca aagatcagac cttgttgtaa atagatccgt
      721 gcccgaaagt tataaaagtt gcgataacca aacgcaattc gtttaatcag cttgatcttg
      781 ttatacagcc cctcagtgac gccgtttgag tgggacacct taaaggcatt tgaaataccc
      841 gattggtagc gatggaagat cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa
      901 attgttttaa gggtttcttg aaagcctacc gtatctcgtt taacgaaata atagcgtaat
      961 aattgacaaa aatgatacgc agttcgcaat tcttcgttgt agcttaacag ctcattaacg
     1021 atttctgttt gactaattgg tcgtttaaac agtcgatgat aatgccgttt gagtgcctcc
     1081 catgtcgtgg aatctttgag taagagtttc caatatcgct tgagccgacg gtacttcttc
     1141 tgattttcag agtgtgaggt tcgaaaacga ttcatgatct ggacacgtaa ttgattgaag
     1201 ctgcgattga tgtgttggac aatatgaaag cggtcagtta cgatctccgc atttggaaag
     1261 accgttttaa gcaactgaca atagctggca ttcatgtcca tcactaggta cttgaccttt
     1321 aaacgggcct ttctaggaaa gcgcaggaag tattgagtta gtttgtaaag tctgcgatct
     1381 tcaagggtcg taaatagctg attggttgtc ccatcaacac aaataaaact catggaacca
     1441 gaagtggcaa cattgtgtac ttttctttgc aatcttgtgt acaaaactat tgcaaaacac
     1501 agcgcataag aatccttaca aaacagtaga tgaagcaacc caattagatc ttgttatagt
     1561 tgttaatcag ctcttaacgg gttacgattc aaaatgggtc aacaccctat acttagataa
     1621 gtcacttgaa tatcagaaca taattcaggc tttttcacgc acaaatcgtc ttaatggtcc
     1681 tgaaaaaccg ttcggaacca ttcgttatta tcgtaaagca catacgatgg aacagaacat
     1741 tgaagaggct attgagttat actcaggcaa acaagcaaaa gggttatttg ttgaaaaatt
     1801 atataataac ttagaaaagt tgaacgatat attcgaagaa attaagtccg tatttaatat
     1861 ttctggcata gaaaacttca gtcatttacc ggaggttcca ggggaaatta agcaatttgt
     1921 aaatctttat aatcaactaa tccattgtta tgaatcagcg atgatacagg gattcacatg
     1981 ggcaaaatcg acttatgaaa tccaggatta tgaacctata acagtagtaa tcacagaata
     2041 tgaatttaat atcttggcac aacgttacag cgagatagag agagaaccta gagacggaaa
     2101 tgatagtata gcgtatgaca ttgattacca attacctgaa ggtagtcgta gtcgtattaa
     2161 ttatgagtat ttaaacacaa acttcaaaaa atacatgaat ataaaagaag aacgtgattc
     2221 gaatttaatt caatcattgt taaacgactt acatcgcaca tttgcgcaat taccagtgga
     2281 gttacagcca tatgctgaga tgattctaaa tgatgtaaat aatggggaat tagttatcaa
     2341 agaaggatgg gaatttactg attacctcaa tgaatatcag cttagtgaag aggacgattt
     2401 tatccaagat ttagtggatt caaccaatat taatgaggca ttattacgtg agatgttgga
     2461 tttacgctta accgaggtaa acattaatga atatagtcga tttgataaat taaaatcaag
     2521 tgttaacgta ggtcatttca gtggatatat cgagaaaaat ttaggtaaaa tggtaatacc
     2581 aatcaaggta aatatgctaa ttgacagatt attgcgtgca tttttattag aagatgtatt
     2641 cgatgttaca gagtacataa agaattactt taactagagt acacctacat cttatagtac
     2701 ataaaaaaat tcatcttttt tagtttaata attcaccaaa aaggtgagta atgccatttt
     2761 tgaaaattga gcttgtttta tgtctacatc tgtatccgta tataatccaa aggattataa
     2821 actcagaaga gtctttattt tttctgaata taactactgt atcaaaaaca atcgattatg
     2881 agacaaaaca aaatgacctc ttttatttaa gaaaggaggt cattttttgt atcataaatt
     2941 gtattaaaat caatgtatca ctttttaatc aattttaaga ccataaattg cgtactaaaa
     3001 gaaattctgc aattcaaaat aaagcatttt aagccatttt caaatttaag tgcacccatc
     3061 tacccgtcaa atcactttat tttccactcc agtagtttcc taattcaact gtgtgcaaaa
     3121 cgatttcttc taaacaaatc aagacaaact tataaacctt catcccagca ccataattat
     3181 ttaaatcata ggcatttttc gaaattagta tatgtactca agtttcactg cagattattt
     3241 atatttttta gagaaataaa tttttcctat tgacaaacac aaagaaagcg gatacaatac
     3301 aattatgtca catgacgtta tatgacgtta tgtaatatct ttacatttaa tcagttactt
     3361 ttattttgga taaaattaac atttttaaaa ttttaaggag gaaatgttga tggatgtagc
     3421 aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag atttctggaa tttatttggt
     3481 ggcttgtggc ggttcgctgg tagatatgta tgtctcagat tattttctta aagccgaagc
     3541 atcgaagtgt attacaggct tatatacagc gaacgaattt aaccatgtaa ccccaaagca
     3601 attagatgat aaatctgttg tcattctatg ttctcatgga ggaaatacag cagaaacggt
     3661 aaaagcagga gaagtcgctc aagctagagg agctgtgact gttggtctga ctcataataa
     3721 aactgcggat ttattaaagg tttctgatca tagtttcctt tacgaatggg gagatgatgc
     3781 aaaaatagtc aataatccta tggccatcat tcttgattta actgtatctt tggtcaatgt
     3841 taccgaaaca tttgaacatt acggtaaatt cagagaaggc atgaaggtta tcgataccgt
     3901 aattgaaaat ggcattaagc aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa
     3961 agatgaaaca atgttttata tcttaggtag tggaccatca tatggacatg cttatggttt
     4021 ttcaatttgc tctttgatgg aaatgcaatg gcttgattca acttctgttc attctggaga
     4081 attcttccat ggaccttttg aagtaacaga caaagatacg aattttattc tcttgatgag
     4141 cattggacgc actcgtgctt tagatgaacg ggcaaaacga ttccttgata aatatgcaga
     4201 aagagtagaa atcgttgatg caaaggaact aggattggat atgatatcag atgaagtttc
     4261 tgaattcttc aatcccattt tattctacag tattctttca gaatatcgtt cagctttagc
     4321 agatgcacgc aatcatgatt tagatgttcg tcgctatatg gggaaagtgg agtactagga
     4381 ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg
     4441 gaattatgta tcctggagga aattcattga attttgcagt ttttgcaaaa aaactgggac
     4501 atgaaagtag ctttatggga gtttttggct cagatgagga ggcagctcat gttttatcag
     4561 caataaaaaa aataggactg gacaacagtc actctcgctt tgaaaaaggc gagaatggct
     4621 gtgccagagt gaagattgat gagggcgacc ggattttcct tggcagcaat tcgggaggtg
     4681 ttactcgtga atacccgatt cagcttactg aagaagacag agaatattta aaccagtttg
     4741 aattgattca tatgggatta tatagccatg tcaatcatct attagaagag ttacaaaatg
     4801 tttctggaaa gatttcttat gatttttctg acgactttac ggaagaagag gttcagcggg
     4861 caattgataa agttgatttt ggcttctttt ctatagaaga cttttccgat gaagaagtaa
     4921 agcagttttt gatgaagcat ttttgtgagc gaaatgatgt attgattgca acccgaggag
     4981 aaaaaacggc tattggttat gatggagcaa agttttatga agtgaacccc gtgctagaag
     5041 aaccagttga tacaatggca gcgggagatt ctttcttaac agctttccta ctttattttc
     5101 tcgaagggga cgatatagtc accgcgatga aaaaggggaa tgaatttgct gggaagtctt
     5161 gtctggttga cggttcgttc ggctttggta ttcattacga ttgagcttgt tttaaattcc
     5221 tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat
     5281 acaaatgcac aattaccatt gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat
     5341 acaggggagt atcccgagaa tacgaaaatt cccgctgaat cagagctgat agaaagttat
     5401 gaggtcagcc gaatcacagt gagaaaagca gtagaagaac tggtagaaga agggtaccta
     5461 cttaaaaaac aagggaaagg aacttttgtc aaccggcata aagtttttcg taaaattgaa
     5521 tatgttatgg gtttcagtga gagttgtaaa tctaatggtt ttacaccgac tagcattgtt
     5581 ttggaacgca agatcattcc agcaactgaa gggatggcaa aaaaactcga tatcgctcaa
     5641 ggtgatgatg ttttatatat ccagagaaag cgaatggcgg ataacatccc cattatgtta
     5701 gaaaacaatt atttttctta tgaaagatat agtttcttaa tggaggaaga gctgactgat
     5761 tctttgtatg aagtacttaa agtccaaggt accttagcca tcaatcctgg ggaaactgca
     5821 cttgaaattg tcacagctga tgaccaaatt gccaaaataa tggaagtagc tgttgggacg
     5881 ccctttttct ttatggatac tttaatcaac gatcaaaaca acataccaat tcatgtaggt
     5941 aatcaatatt acctgggaga agtatatcgt ttttacttat gatttcctgt tttgatttca
     6001 aggagtagat tttatttatg aagcaagtat tgcggtcaaa aatgattttt gatggagagc
     6061 agccaccttt caaaggttat attgtaatca atgaagggat cattgaaaag gtggaaaaca
     6121 actggaatta cagtgatcta ttaaatgaaa acacggaatt aattgcttat gatgatcaat
     6181 tcgttatgcc agcaattcat gataatcatg tatttttcag tggttatttg gctatgaatg
     6241 ctggcataga tttaagcgat acatcttcag ctaacgaggc tgtagagcgt attaaagctg
     6301 taatgaacca taaaaaagca gatgaaccaa tttatgctca tggctgggat gctgccctgt
     6361 ggaaaactga cccatcagaa gagctattga atgaaataaa ccactttggt ccaatcacag
     6421 cgatcgacaa aaatcgcagc cattgttgga tgaatgctat tgctaagcag acttatggtt
     6481 ttacagaaaa agaaaccagt gctgaaagtc gagtattatt aattaaggaa atgctagcaa
     6541 acaaaaagct tttagcagag gtttacgctg aatttgaagc attactgctt tcaaaaggtg
     6601 ttatatcaat gaaagaaatc gtttttgatg acgcagaatt tctttctaat atacctcaga
     6661 ggaaaatact tagtaaccta tatgtgcagg cagtccaaga acctctacag catgaacttc
     6721 tatatgagta taaggaaaaa gttttccctg aaaaggtccg ctttggtgga gtgaagatca
     6781 tggtagatgg tgttgttgcg gatagaagtg gagatattta cggtgtttat cagtcaggag
     6841 tagcttcacc cgagattgac tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag
     6901 tagagattcc ttgctgtttg acaacggaag gagataaagc gggttataaa gctgcgtcaa
     6961 ttcttgtcga atttggaaag cggttacctg aaggcgtttt taattcgatt agtgatttgg
     7021 aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat
     7081 atccacaaat tctcggcttg aatcaaacgt tagaagaggc ttacatgtca actgttataa
     7141 aaaatgagtc aagtccacaa tcttgtgtaa aagttcaacc tcatcaaaag cacaatgttt
     7201 tgcgttgtga acctaactgc catgcctttc tttctgagga gagaggtcag gagcagtagc
     7261 gcagcgactc ttgatgtctc tcctcagaaa gaagagactc gcacaggttc atggtgctaa
     7321 atgttattcg aattaaattc taaatatttt cgtggagaag ccagccactc agtgtctttt
     7381 tccatcaaaa gtgttccaat tatccgagtg atggaattga tatttggaaa aatccgaatc
     7441 actcgttctc tccggcgaat ctcttcgttc aaccgttcca acacattggt tgtacgaatc
     7501 cttcggcgga tattttcagg aaaagacaaa atttgaatag catcttcaaa gccgttttct
     7561 aaaatgtcgc aagcagcact taattttgaa tcacaaccat atttttccaa gaagtgctct
     7621 tttctttcac gtgtcaattc aagttcagag gctttaaaga tactcttcaa ctcgtctttt
     7681 acatcagaag aaactttctt tggcagctta tcaaaaatat ttctcaaaaa gtgggcttgg
     7741 catctttgcc agctcgcacc taaaaagttc ttgcgaacag ccttaactaa ccctttgtgt
     7801 gcatcagaaa tgaccagttt tacaccgaac aaacctcgtt gaacgaacga ttgaaaaacg
     7861 gtatcccaat tatcttccga ttcgccgtct tgaacatcaa aaccaagaat cgtacgtcgt
     7921 ccatcagaat caatgccgat accaatatat acaccttttg agacaatacg atgattttcc
     7981 cttactttca tatacagggc atcacacatg aaaaatggat attccgagcc ttctaggctc
     8041 cggtttctcc actcaaaaac aaatggatcc aactgtttca tcagatttga aacgaatgat
     8101 ttagaaacag tcgcaccatc cgttagcagt tcaactgttt tagttacttt gcgagtggag
     8161 acaccagaaa tgaccatttc aatcatggca gcgatcaaag ctttttcgct tcgctgatat
     8221 ctttcaaaga tagtaggact aaacttaccg tctctggttc gtggaacatg aagttctaac
     8281 gtaccaattc gagttgtaaa cgaacgatcg tagtaaccat ttctttgtga ttgccgttct
     8341 tctgaacgtt cataggcatt tgcctgaata tattgatttc gttgttcttc catcagctga
     8401 ttaaacatga tagtcataag tgtttttgcg gtatcattcg caccgctttc gtttataatt
     8461 gcttgaattt cttcgctttc aaaagtaaaa tgtacttgag tcattgatat cagtcctatt
     8521 cgagtatgtt ttctgcaaaa aacattgtac tcctaactga gatcaatggc ttttttgatg
     8581 cttcactttt a
//
LOCUS       DAYYAO010000105         8223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000105 DAYYAO010000000
VERSION     DAYYAO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.109"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHARI_002464"
     CDS             476..1339
                     /locus_tag="ACHARI_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8443470.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHARI_002465"
     CDS             complement(1468..3090)
                     /locus_tag="ACHARI_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8443471.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHARI_002466"
     CDS             3339..3860
                     /locus_tag="ACHARI_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8443472.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHARI_002467"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHARI_002467"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8443473.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHARI_002468"
     CDS             5941..6759
                     /locus_tag="ACHARI_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8443474.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHARI_002469"
     CDS             complement(6862..7059)
                     /locus_tag="ACHARI_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443475.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHARI_002470"
     CDS             7228..7806
                     /locus_tag="ACHARI_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8443476.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHARI_002471"
     CDS             7901..8038
                     /locus_tag="ACHARI_002471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443477.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2470 a         1481 c         1619 g         2653 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaa
//
LOCUS       DAYYAO010000106         8006 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000106 DAYYAO010000000
VERSION     DAYYAO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.110"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..2679
                     /locus_tag="ACHARI_002472"
     CDS             13..2679
                     /locus_tag="ACHARI_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8443478.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3043..3795
                     /locus_tag="ACHARI_002473"
     CDS             3043..3795
                     /locus_tag="ACHARI_002473"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8443479.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(3817..4971)
                     /locus_tag="ACHARI_002474"
     CDS             complement(3817..4971)
                     /locus_tag="ACHARI_002474"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8443480.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            5156..7597
                     /locus_tag="ACHARI_002475"
     CDS             5156..7597
                     /locus_tag="ACHARI_002475"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8443481.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(7754..>8006)
                     /locus_tag="ACHARI_002476"
     CDS             complement(7754..>8006)
                     /locus_tag="ACHARI_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8443482.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         2595 a         1443 c         1749 g         2219 t
ORIGIN      
        1 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
       61 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
      121 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
      181 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
      241 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
      301 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
      361 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
      421 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
      481 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
      541 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
      601 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
      661 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
      721 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
      781 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
      841 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
      901 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
      961 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     1021 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     1081 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     1141 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     1201 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     1261 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     1321 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     1381 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     1441 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     1501 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     1561 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     1621 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     1681 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     1741 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     1801 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     1861 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     1921 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     1981 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     2041 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     2101 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     2161 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     2221 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     2281 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     2341 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     2401 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     2461 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     2521 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     2581 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     2641 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     2701 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     2761 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     2821 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     2881 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     2941 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     3001 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     3061 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     3121 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     3181 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     3241 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     3301 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     3361 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     3421 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     3481 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     3541 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     3601 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     3661 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     3721 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     3781 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     3841 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     3901 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     3961 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     4021 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     4081 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     4141 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     4201 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     4261 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     4321 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     4381 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     4441 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     4501 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ctgtaacgag caatatcact
     4561 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     4621 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     4681 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     4741 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     4801 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     4861 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     4921 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     4981 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     5041 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     5101 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     5161 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     5221 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     5281 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     5341 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     5401 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     5461 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     5521 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     5581 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     5641 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     5701 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     5761 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     5821 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     5881 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     5941 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     6001 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     6061 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     6121 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     6181 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     6241 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     6301 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     6361 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     6421 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     6481 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     6541 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     6601 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     6661 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     6721 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     6781 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     6841 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     6901 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     6961 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     7021 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     7081 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     7141 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     7201 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     7261 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     7321 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     7381 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     7441 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     7501 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     7561 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     7621 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttggc tcttcgtcaa
     7681 attgtgttgg cgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc
     7741 gccgcatatt tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa
     7801 agttataaaa gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca
     7861 gcccctcagt gacgccgttt gagtggggca ccttaaaggc atttgaaata cccgattggt
     7921 agcgatggaa gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt
     7981 taagggtttc ttgaaagcct tccgta
//
LOCUS       DAYYAO010000107         8003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000107 DAYYAO010000000
VERSION     DAYYAO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.111"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..2018
                     /locus_tag="ACHARI_002477"
     CDS             252..2018
                     /locus_tag="ACHARI_002477"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8443483.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2120..3379
                     /locus_tag="ACHARI_002478"
     CDS             2120..3379
                     /locus_tag="ACHARI_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8443484.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3526..4827
                     /locus_tag="ACHARI_002479"
     CDS             3526..4827
                     /locus_tag="ACHARI_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8443485.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4830..5681
                     /locus_tag="ACHARI_002480"
     CDS             4830..5681
                     /locus_tag="ACHARI_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8443486.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5694..6509
                     /locus_tag="ACHARI_002481"
     CDS             5694..6509
                     /locus_tag="ACHARI_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8443487.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6660..7826
                     /locus_tag="ACHARI_002482"
     CDS             6660..7826
                     /locus_tag="ACHARI_002482"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8443488.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2613 a         1376 c         1591 g         2423 t
ORIGIN      
        1 ccaaaaactg ttaaaaaact tttactttag aaaattggag aaattgatcg cttaagataa
       61 attcaggaca ctaaatagta caggaagtag actaaaactt ttaaaagttt taagtctatc
      121 tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa actgttaccg ataacatttt
      181 tgaaagcgtt gtcttttgct tctctatcac ttatagtgat gacgtcatat taaaacacaa
      241 ggaggaaatc aatggataca gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc
      301 tatatacgaa tacaagattt cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag
      361 tatatgtgct atgtggagat ccatatacca taacaactga aaaatggtat caaaaacaac
      421 gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt gaagtgacga
      481 gcgaaacaag tcgcttacag tatgcgttcc atgttgttgg ggaagataac acggactgtt
      541 tttacggtga tcaaggaatt tttccttatc gagaaaatgt gattactgaa ccaaattttt
      601 attttcgaat accttatttt catcaaatcg atcgattcac tattccagaa tgggtaaaag
      661 aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc
      721 ccgaacacac tttaccttgg ggaagtaaag atccagaccg agaagacttt ttcgggggag
      781 atttgcaagg tgtgatggat catttggatt acttgacgga ccttggcatc aatggcatct
      841 atttttgtcc catatttaaa gccacatcca atcataaata cgatacgatt gattattatg
      901 aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa cttgattgat gaagcacata
      961 aaagagggat acgtatcatg ttcgatgctg tattcaatca tatgggtgtc cattcaccac
     1021 agtggcaaga cgttctcaaa aacggagaaa aatcgatcta taaagactgg ttccacatcc
     1081 gttttttccc agtagattct tatcagatga ctgacttacc agaaactgct gaaaatatcc
     1141 catatgatac ttttgctttt actccattca tgccgaaact aaatacagca aatccagaag
     1201 tgcaaaaata tttgctggat atcgcaactt attggataaa agaattcgat attgatggat
     1261 ggcgtcttga tgttgctaat gaagtcgatc atcatttttg gaaaaaattc agagaagcag
     1321 ttacagaaat caaaccagac atctatatcc ttggagagat ttggcattcc tctcaagctt
     1381 ggctgcaagg ggatgaattc catgcagtga tgaattatgc atttacagat tcgattaaag
     1441 attattttgc aaaaaagaaa atcacagctt cacaaatggt cagtggaatg aatcatcagc
     1501 aaatgcttta cagagatcag gtgaacgaag gaacattcaa cttattagat tctcatgata
     1561 ctgccaggat cttgaccctt tgccaaggta acaaagaatt gatgaagtct gtgatggcct
     1621 ttatgtttct tcaaaaaggg tctccttgta tctattacgg gacagaaata gggatgactg
     1681 gtgaagatga cccggattgc cgcaaatgta tgatatggga gaaggaggaa caagatctag
     1741 agctattcgg ttttataaaa gaacttgttt ctctaaggaa acagttgtct aagattattt
     1801 ctgaaggttc tactcagtgg ctaatagtaa atgatcgtga agataagctc tatttcacac
     1861 ggaaactaga gggacagatc atctatgttt actttaatca gtcaaaagaa ccttgggtag
     1921 tagaacagga aaatgaggtg attcttagtc aaaattgtca gttgttagaa gacgggaaag
     1981 cagagatacg acaatacgga tttttgatca tgtgttgaaa actgttaccg gtaacaacaa
     2041 gaaagcgttt gcaaatgaag atttgtgccc attataataa gggtgtaaca gcacgttgaa
     2101 tacaaaggag gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat
     2161 ctctttagga atcttaggcg gattagcggc ttgtggcaat ggtaactctt cagcagaagg
     2221 ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat tctggatata aagattatat
     2281 aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa
     2341 ggacatgttc gatcagttgg aatcattagc gttagatggg cctgctggaa agggacctga
     2401 cgttatgatg gcagcatatg accggatcgg tgcgttagga cagcaaggac atctggcgga
     2461 agtaaaactg ggaaatgaag cagcttacga tgaaacagac aaagcccaag tgacttatga
     2521 tggaaaaatc tacggagaac ctgcagtaat cgagaccctt gttttgtact ataacaaaga
     2581 tttagtcgat acagcaccat caacgtttaa agatttggag aatctatcca aagacagtcg
     2641 ttttgctttt gagtcagaag caggaaaaaa tacaggattt ttagctaaat ggactgactt
     2701 ttattattcc tatggtttaa ttgcaggtta cggtggctat gtatttggtg atgatggcac
     2761 aaatccttca gatatcggtt tgaataatgc aggtgctgta gaaggaatct cttatgcaac
     2821 agattggttc aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg caggagactt
     2881 cgccagccag caattcatgt ccaataaaac tgcagcaatc attgacggtc catggcaagc
     2941 gcaaacatac aaagaaaaca atatcaatta tggtgtagcc aaaattccta ctctaaataa
     3001 tggacagcct tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca attatgctaa
     3061 aaacaaagac ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa
     3121 attctttgaa atggtaaatg aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc
     3181 taaaaacgat gaattaacaa cagctgtgat tgaacaatat gaaacagctc aagcgatgcc
     3241 aaacatccca gaaatgggcg aagtttggac aggtgcagaa aacttgatgt tcgatgcagc
     3301 atcaggttca aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc
     3361 catagaacaa aaatactgat taaaatgata accgtccatt tttataaaaa atggaacagg
     3421 tgcttcagat aaaagtggca agagcgagat ggaaaattat cttgctcttg ttgccattat
     3481 cttaaaagca gtttgcggtt tagcaagttt agagggggca ttcagatgca gcaagataaa
     3541 acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg gatggggcag
     3601 tttgtaaatg gccaagcttt taaaggtatc gtttttttcg ccatattcgt cctattcatt
     3661 ggtcagatgc ttctgggcgg atttcatgca ttggtaggat tggttacttt gggaagtata
     3721 ccaatggaag atcattcatt atttattttg attgaaggca ctttgcaatt catcattaca
     3781 atcattttct tgatttttta cggtttgaat cttttagacg cttatcgagt agcgaagatg
     3841 cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag tcatacataa tataggtgat
     3901 gaaggttttc cttatctatt gacacttccc gcttatcttc tcatgatttt tacgatcatt
     3961 ttcccagtcc tagttacatt gttcactgca tttactaatt atgacttcaa tcatattcca
     4021 ccagccagtt taattgattg ggtaggggct aaaaactttt ttagtatctt ctttttaagc
     4081 tcatatcgta atactttttt tgcagtcttt tcgtggaccg tgatttggac tttatgtgca
     4141 acaactttgc aaatcaccct tggtattctc acagcggttg ttgctcacca gaaatttatt
     4201 aaattcaaac gtattttcgg tatcattttc ttattaccat gggcggtccc agcttttatt
     4261 actatcatga gtttttcgaa tatattcaac gatagcgtag gtgccatcaa tactcaagtg
     4321 attcctttca taaaccagct tccttttgta gatatcagtg caatctcttg gaaaacggac
     4381 ccgaactgga cgaaagtagc aattatctta gtgcagggct ggttaggatt tccctatatc
     4441 tatgtgatgg ttacaggaat cttacaatcg atttcagaag acttatatga agcagcaaaa
     4501 atcgacggag caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta
     4561 gtggcagcgc caacatttat tacacagtat acggggaatt tcaataattt ctccatgatc
     4621 tatctgttta acaatggcgg tcccggaagc gtaggcggag gagctggttc tactgatatt
     4681 cttatttcct ggatctataa acttacgact ggaacttctc cgcagtattc tatggcttca
     4741 gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag
     4801 acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc
     4861 aaaaacgact ttctcacttt tttacttact tttttctttt aatactgtca gtcattatca
     4921 tctatccgct tttgatcaca gcaagtactg ccttcaagtc aggaaatatt gcagctttta
     4981 gtttagatct ttcaagtgac tggactttag gaaactttac tcggctattc agtgaaacac
     5041 tttacgggac ttggtataaa aatacattga tcatttctgt cctaacaatg atcgtacaag
     5101 tgactgtagt aactttggcg ggctatactt atagtcgcta tcgttttatc ggccgcaaat
     5161 caagtttgat gttcttttta gtgattcaga tggtaccgac tatggcagca ttgactgcct
     5221 tttatgtcat ggctttctta ttagaagcgc tcgataagta ctggttcttg acaatgatct
     5281 atataggcgg tggaattcca atgaatactt ggctgatgaa aggatacttt gacactgtac
     5341 caatagattt agatgaatct gctaaacttg atggggcagg gcatttcagg atttttttcc
     5401 agattgtatt accgcttgtc aaaccgatgc tcgccgttca agcattatgg gcttttatgg
     5461 gaccttttgg ggatttcatg ttagctaagt tcctgcttcg aacacctgaa aatcagactg
     5521 ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa ccaaagagtc tctctttttg
     5581 ctgctggggc aattttgatt gcactaccga tttgtctatt attcttcttt ttacaaaaga
     5641 actttgttac cggcctgaca tctggcggaa caaaaggata ggagcagagt atcatgcaaa
     5701 catttccgtt aaactatttt tccgcattac gtacgccaac tcaagtgttt gcgggacgca
     5761 aactattatc atggcctaaa tttttcttga tatttgtatt tttagtttcc ttgatggtca
     5821 tgcctgtgac tcttttttat gccaatcaga tccaagcgat tccattagaa caatttctat
     5881 ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt gaattatctg
     5941 aaactgggtt acaagcatcg caacaaacga tagctgtgac ccctgaaatt ttggtaggtg
     6001 tgtctttatc tgagaaacaa caatccgatc atggaacttt tattgatttt gagaaggaac
     6061 aatgggtgat ccaacaaaag gataaatctg gtatacgccg ctatacgatg aattatagcc
     6121 catctttcca accagattct gtacgtacgc ccgaagattt ccaacgattt ttagaaagag
     6181 aattttatgc cagtaatcgt ccaacaatca ttttatctta tagtttaagt cttggtcttg
     6241 tcctatttgt catgacgagt ctcattttat tcggggcttc ctttttcttg tggatgacaa
     6301 gaaaaagcca actttcatcc attcatacat tcaaagagag tgcaaatttg atgctgaatg
     6361 ttatgggaat aggttccatg gcagcagcag ttgtagggtt tatccacttt gactttatct
     6421 tgatgctaag tgtccaaaca acgataactg tcttgctgct tttatgggtt tttgtcaaaa
     6481 caagattcaa agataagcgt gtcgaatgac aagtttaagc gatttatgcg acaatgaatg
     6541 tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct gaaaaaatta aacttcttag
     6601 ttgattcttt tcttttttag tattaacgta atagggtagt ttttttaagg aggaaaatca
     6661 tgttagagaa aactttatac aatcaattat tttcacgttc tttctcgctc cctgtcgaag
     6721 taaattattg ggacggtaca acgaaacaat acggagatac ggacactcct ccccaaatca
     6781 aaatcaatat ccatgaagaa atcccaatga aagagattac aagtaacgct tctttggcac
     6841 taggagaagc ctatatggat catcgaatcg agatcgaggg aagtatccaa gcattgatca
     6901 acgatgctta tcagcaagca aacagctttt tacgaggaaa agattatcta aaatggctgc
     6961 caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga tatccatgcg cattatgact
     7021 taggtaacga cttttataaa ctttggctag atccaacttt gacttattct tgtgcttatt
     7081 tcaatacacc tgaagatacg ttggaacaag cacaaatcaa taaagtccat catatcttag
     7141 acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat cggttgtggc tggggaacat
     7201 tgatgttcac tgctaccaaa gagtataatg tcaaggcaac tgggatcaca ctaagtgaag
     7261 aacaatacga tttgattacc aaaagaataa aagaagaaca tttagaagat aaatgccgtg
     7321 tattattaat ggattaccgc gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag
     7381 gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt
     7441 tattgaaacc aaaaggaaca gctttgatcc atggaatcag ccgacaacaa ggcggtgcga
     7501 caaatgcttg gatcaataaa tatattttcc ctggcggtta tgtccctggt gttactgaat
     7561 tagttgataa tattacaaaa aacgatttgc aactgattga tttggaaagc ttacgtcgtg
     7621 actaccaatt gacattagaa cattggacaa agaattttca tcaagtccaa gatcaagtca
     7681 cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct atatttacaa gcttgtgcag
     7741 cttcatttga atcaagtaat atcgatgtga ttcaatattt gcttgttcaa cctggaaaca
     7801 atgacatccc aatgcatcgt gcataattga ggcaaaagta ctgtttaaag actatttaaa
     7861 aagaagctaa gttttatgat gctccagaag tgaacttttt tgtctaactt ctaggattgt
     7921 ctctaaagct tagtttcttt ttctttatat tttcataata aaagtttggt tctttaattt
     7981 ttaaacatga cagaaaattt ttt
//
LOCUS       DAYYAO010000108         7972 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000108 DAYYAO010000000
VERSION     DAYYAO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.112"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..1656
                     /locus_tag="ACHARI_002483"
     CDS             19..1656
                     /locus_tag="ACHARI_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443489.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1801..2541)
                     /gene="truA"
                     /locus_tag="ACHARI_002484"
     CDS             complement(1801..2541)
                     /gene="truA"
                     /locus_tag="ACHARI_002484"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8443490.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2732..3367)
                     /locus_tag="ACHARI_002485"
     CDS             complement(2732..3367)
                     /locus_tag="ACHARI_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8443491.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3514..4734)
                     /gene="sstT"
                     /locus_tag="ACHARI_002486"
     CDS             complement(3514..4734)
                     /gene="sstT"
                     /locus_tag="ACHARI_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8443492.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5302..>7972
                     /locus_tag="ACHARI_002487"
     CDS             5302..>7972
                     /locus_tag="ACHARI_002487"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8443493.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2612 a         1492 c         1238 g         2630 t
ORIGIN      
        1 tttaaatagg agaaaaaaat gaaaaaaaac aaaaaccaaa taatagatat catttttatg
       61 ttatttctaa gttttgctta cgtcatccct ctattcatgt ctatgggaat ctatcatagt
      121 gtcaatcagg atacttactt tcatttatct cgaatcatag gactagataa tgtatggagt
      181 tctccagtaa acttcaataa cttcgatcat catggaacaa tgatgaatat tttttatcca
      241 tggcttactc tttatccagc ttttttattt tataaaatga tgggaaatct tgttttaagt
      301 tataatatct actatttttt tatcactttt ttaacaatgg tcgtttctta tttttctatg
      361 aaacaaataa aaaacaatcg ttatatttcg ctgttatttt cgattattta tacattttcc
      421 gcctatcgtg ccattgacat attccatcgt gcttcattgg gagaagctgt cgctttaaca
      481 tttttgcctt tgatactaat ggggtgttac gaaatttata ttagagatta ccaaaaatgg
      541 tactggttgt ctattggcat gacgctagta gtgtatactc atttactttc tgtcgctatg
      601 gtttctgtat ttattggagg aacgttgttt ctttcttttt atttttggga ccaaaaaatc
      661 gctagactct taagtctttt aaaagctacc gtcctcacct tttttttaag tgcgggattc
      721 ttaattcctt ttattcaaca atctagagca caagaattga aagtcccact gggaaaagaa
      781 ttaagtggta tggctcctag tgatatgctg acacacattt taaataataa ctataacaac
      841 tatactattg gattattctt atttctcgga ttgattggtg cttttatttt tataaaaaaa
      901 ttaactgctg atgatttatt tattttcttt cttggtgtat ttgttctttt ttgttcaacc
      961 aatttatttc cttggcaact ttttaaccat acaccagtta agagtctaca atttgtctgg
     1021 cggttaaatg gtttttcttc tttatttata gcctatacga tgagtatagt gatctattat
     1081 attttttcta ctaaaaataa ttcatggaaa ataatggtct ttacgtttct tgcactaata
     1141 ttgcatctct caggagtaag caattctatc catgctaaca aagattcatt aacacttata
     1201 gcacctgaag acgctgttgc tatagctgaa aattacaatc atactgatta tgcaaacaaa
     1261 gagtcaatct atcatcctga tatgattaat aatgatcaat atttactagg taaccaggaa
     1321 ataaatccaa caactcattt tatgagtaac aaacttacaa ttgaattaga taattcgaat
     1381 aataaaaata ctgtcttgac gacacctatt tatcgttata aagggcaagt ggcttctatt
     1441 aatggcaaac tagtacaaac aaaattatct aagtttggca caactgaatt aactattcct
     1501 ccaggtataa ataaagtcgt tattacttat cagtacacaa aactagcaat tgcttcaaga
     1561 tatttatcta tcgttacttt gattttattt ttactttatc gatttacttt ttctaaacaa
     1621 aaacaaccga gaagagaagt acaacattct cactagattt ttatttaaga aagacttttt
     1681 agctgtacac ataaacaggc taaaaagtct ttttttatat ttctttaatt tgcttcatac
     1741 attatttaac atcgatattt atactgtgaa acatgcgttt catccattat caaagtattt
     1801 ttatcgataa taaacttcat ccaaaaacag accttgtgca gggacggttt ctcctgcatg
     1861 ttgccgcact ttgctttcaa atatttcatc gattgtttct atcggcatgg cacctgtgcc
     1921 gatttctagt aatgtcccca tgataatccg caccatttta tataagaatc cgtctccgac
     1981 aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat aattcatcaa tcgtacgcac
     2041 tgtggatttt tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg tacccactaa
     2101 tttttcgcaa gctgcattca gcttgtccat atccaactct tgagggaaat aaaagctgtg
     2161 gtttcgctca aatgctgaag gaatcacgtt gttccaaaca taatagctgt attttttgcc
     2221 caccgcatta tatcttgaat ggaagcgctc aggcatttct tcgattcttt tgatgacaat
     2281 atcccgcgga aggtaacgat tcataaagtc gatcatttct gccagtccaa tttctgtgtt
     2341 tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca gcatctgttc ttcctgatcc
     2401 aataatttct atctcttcat ttgtcatctg atgcaatact ttttcgattt ttccttgaat
     2461 ggttttatcg tcatctccaa gtctttgcca tcctgaatat ctttttccgt catattcgat
     2521 tgtcaacttg atgtttctca tattttagtg gctcctctgt cttccatttg gtcttctctc
     2581 taccttttta agattgtagc acaaaatata gctcctttct tctctaaaac agaagctata
     2641 caagaaaact gtggaaatat atatttcaga aaaacaaaaa acagccaccg gatgaagtat
     2701 ccgctgactg ttcaatttga cggctcaagt tttactgttt gagatgcaag atactcaacc
     2761 aggcaatggg cacataatta aagaaaaaac tgattctttg gcaaaaacct attttattca
     2821 gtaatggcca agaatttata gaaggaattc ttgccagtcc ataaatcgcc gcaaaaagca
     2881 aactgatcac agacagtcca aggtataagt ggcttacgtc catttgccca ttttttcgat
     2941 agatcaagaa aagaagaact ggaaacaata aaaaaccact gtaacctaaa cctgatccga
     3001 tattatggac ccatgtcgag acattccacg aagattcatg ggtattgata ctgaacaaac
     3061 ctgtaaagat acaatcacct ataccataga acgcgatggc tgctgtaact agggtagcta
     3121 acacacgaga tgtacttaca aatgcagcat acaatgcagg caatgacagg atgaaaaaga
     3181 tccctgaaac cacagaccaa ataagaaacg cttttcttac aggactggaa acctctccaa
     3241 atacactgat cacgctcttc atttgatcta attcaggata atacaatcct aatacgtacg
     3301 gagtcaaaaa atcactcagc acgcctaata ataaaaagaa aaaaccaaat ttacgtaaaa
     3361 aatccatgat acgcctccaa taacaattac ctatgctttt aattgttatt ataagcgact
     3421 ttatcttgat ttaacagaaa cctctattta cacgaacatc tatataactc attaaaaact
     3481 cgaatcattt taaaaatact aaatatctaa cgttcaacgt tcacttaatc catctaaaca
     3541 atctttcaat ttgatttttt ccccacttct tttacgcgcg cctagctctc ctattgcagt
     3601 aaacaacaga tcacttgacg aatttaaggc agtttccatt gaatcctgga taacaccgat
     3661 aataaatcca ataccgacga tttgcatcgc gacatcatta gagataccaa acaaactaca
     3721 tgctaatgga atcaataaca gtgaaccgcc cgcaacacct gaagctccac aagcagctaa
     3781 agcagacaaa atacataaaa ggactttcaa aacaaaaggt gcttcaatcc ctaaagaatg
     3841 tacagcagtc aacgtcatca aggaaatggt aatcgcggca ccgcccatat tgatcgtagc
     3901 acctaacggg atcgatatgg aataagactc ttttgtcaat ttcaatcttt cagccaacat
     3961 catgtttatc gggatatttg cggcagaact tcgggtaaag aaagcgggaa gtgcgctttc
     4021 tttcaaacaa aagaaaatca atggataggg attttcttta ataaacaaaa acgtgaggaa
     4081 tgggtacaca actaatgcga caaacagcat cgtaccgatc aaaacaagta aaagctgcag
     4141 atactctgcc aatccagcta ttccagtcgt agcaattgat tgataaacca atcctaaaat
     4201 ccctatcgga gcaaatgcaa taatcacttg tacgacttgt gacaacgcta tagatatctg
     4261 atccaccaac tcttttgtcg attctgaacg tgctcgaaaa gctagtccca acaatacagc
     4321 ccagaataag acgccaaggt aattcgcatc aataagagat tgaacaggat tttgtgtgac
     4381 attcgtcaac atagtagata aaacactttc caaactttgc ggtgcttttt cagatactgt
     4441 ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca ataactgcta caagtgcaga
     4501 aagcaatgtt cccactaaat ataagatcaa aataggcttc acgaatgtct tattcccaat
     4561 tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg cgattgcctt
     4621 caagcaactg acaaacaacg ttcccaatat tgaaataaat gtccatgtgg ggaaaaagat
     4681 accaaacaat attccgacta aaataccaac aacaatacgt tgaactaacg acatatccaa
     4741 aaacttctct aacaaatgac caccctttca caattttaac aatcattgta tcattttatg
     4801 caaagaaact aaaggtataa ttttctaaaa attctaacaa tacaaacaac catttcttgc
     4861 taattttgta ctttttctgt ctacacgttg ttttttgaga tattctaagt taggatgtta
     4921 ttaatgggca acttatcagg aaacgttttc ttatgttttt gtccaatttt tttgtgaggt
     4981 gtttattctt gaaaaaaata aagaaggaga gttatggctt cctgacaatc tttgtcgctt
     5041 gtataatgat ggttttacag cgtatattga tttcagcagt cactattcct acatatgcaa
     5101 caaaccctgg tcgattctac atctatacat ttttacaaac ttccgttgtt attggtgcaa
     5161 atttgatccc actttacatg ggttaccaat atcaaaaaat cgcccgttta aaagttttac
     5221 attatttaag tcgttttgcc tttatattcc taatagccac actagtttgc aatatctttt
     5281 ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct aacacctatc
     5341 agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag tattccttat
     5401 acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact attattttta
     5461 acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc attacagaat
     5521 ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt gaaagaatgg
     5581 aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt gattgtttta
     5641 ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa tacagataca
     5701 gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt ctcttcgttt
     5761 ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa ttttcttgct
     5821 gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta tcgcgtatct
     5881 acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa tatcgggcag
     5941 ttttttattt gctatttatt agcagtgtcc ctcctagtta tctttttatt ttatatacaa
     6001 aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt ttgattcgat tgaacaatta
     6061 actcagcgtt ggaaaaaaag aattcattgg ctttttgttc ataaaagtta ttttttagtt
     6121 gcagcatttt tttactgctt taccttcgtg cagattttcg ttttagaacc aaaagaaggt
     6181 tggaaagaaa ctatccaggt tgccttaaaa atatttactc agcgacaagc acccatgatc
     6241 ctgacaatca tcattattat gggatttttc ttcttactat tgttgatttc caatcgtttt
     6301 tggtacgcct tgactgctac cttgatcatt aacttactgc tcactatttc gacagtaatc
     6361 aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt taaaaatact gaccggattg
     6421 tcagaactat tatcgatggt tagcccagtc ttattaatcg tgggcggatt gatccttctc
     6481 ctattattga ttactagtat tattgtacaa cgtagactac aaaaacagta ttccttgaag
     6541 attcattgga aacgtcgatt tatcagtatc gctgtattat taggatgttt ttcgggtgtc
     6601 tttttcatta atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt taaagtaaac
     6661 aagactttct ttaaccagca aggcgccgta aaagataacg gaccagtcat tcaattccta
     6721 aacaatatcg acattaaaat catggaagaa cctgctggct attcgaaaga aaaaattaat
     6781 ggaataatga aaaagtacaa cacaaaggca gatgaaatca accaaacaag aagtgactgg
     6841 ttaaacaatc agacgatcgt tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc
     6901 cctaatctga cagtacctac agaccctatt ccttacatca aaagcattat aacagaagga
     6961 acaggaggat tgatgttatc cgtcggttat ggtggcggaa cagctaacat ggaatgggaa
     7021 ggactgacag gcttgagtat cagtaacctg tctgcttcgt tagttacacc ttatacccag
     7081 ctagtggagc gtcagaaaat aagtccaaat attaccgatc tattcgatga aaaaatagcc
     7141 atccatcctt ttacagctgc tctttatcgc agaaaacaag tgtttgataa atttggtttt
     7201 gacaggttct attatttaga cagtccggat aaattaactt atacggataa aatagaacaa
     7261 agcccacgca tatcggatga ttcagcatac aaagaaacct tgaaagcttt acaaagcaat
     7321 acagaaacgt cacaattcat ccaattatcc actatgcaaa accatatgcc ttatgatgat
     7381 tactatagtg aattgaacta cactgctgaa ggaactgctg tcattcctaa tagaagaaaa
     7441 gaattacaaa cttatatgca aggccttcac tataccgata cagcagtaaa aaaattcatc
     7501 caagaaatcg acaaaatcga taaaccgatt acctttgttt tttatggtga ccatttaccc
     7561 gcaatctata gtgggaatga ttcgaaaaaa tatggattag aacagcacca gacagattat
     7621 ttcatttata acaacaagta tagtcgtgaa caagcagcta cgttaaataa agaagttgtt
     7681 gctcctaata actttggtgc tatggctctt gagcatgcga atatcaaagt aactccttac
     7741 tatgcgttgt tgacagaagt atctaatcat ctacctgcat taacaattga tcctacagaa
     7801 agcttgtcta atcgttttaa tggaaaacag gtctttgtta cagaggatag tcaaattatc
     7861 caagaaaaaa atttaactga gaaacaaaaa gaaattctag aagattatcg tctgatacaa
     7921 tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa aa
//
LOCUS       DAYYAO010000109         7876 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000109 DAYYAO010000000
VERSION     DAYYAO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.114"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHARI_002488"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHARI_002488"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFM8443494.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHARI_002489"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHARI_002489"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFM8443495.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHARI_002490"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHARI_002490"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFM8443496.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACHARI_002491"
     CDS             complement(4028..5044)
                     /locus_tag="ACHARI_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8443497.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACHARI_002492"
     CDS             complement(5146..5298)
                     /locus_tag="ACHARI_002492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443498.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="ACHARI_002493"
     CDS             complement(5315..6634)
                     /locus_tag="ACHARI_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFM8443499.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(6861..7730)
                     /locus_tag="ACHARI_002494"
     CDS             complement(6861..7730)
                     /locus_tag="ACHARI_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8443500.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
BASE COUNT         2097 a         1879 c         1330 g         2570 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt caaaaaaact gcgcgaaaaa ccgcgcagtt tcccccggta tactattttc
     6781 tctttctatc tctagaataa gcgatagtat gaccgttcat aaaatgacga gacatgcgac
     6841 aatcgttaag aaagaaaaag ttaagcgtcg catcgaatgt cagttttatt cttttctttt
     6901 ggctcactga cgaaccacca ttcgattcct atagcatcat agattgtgag tactgtgtgc
     6961 tttttgtcta agaagaaatc aactgatagt tgctgcaagt gagcaatcag tccttccatg
     7021 ctttcagtat cgatcatgaa tttcagaaaa tcaagtccaa tcacttcatc tgtaggcaaa
     7081 tcaattgcgc ctccgctagc ttctgtcaaa ccaacatgga aatctccttc attcaataca
     7141 tggatgccct cttcttcatt atgtaccttc aaaccaagta catgattaag aaattggcgt
     7201 tcttttgtta agttagtaac atttaaatgg attttttcaa aataagaacc tgttgatagt
     7261 ttactgaatt tttcttcaga ttttcttaat aatgcttctt gatccattgt ttctggaatt
     7321 tctctttggt cgttcttatt ctcttcatgg taaatttcca attcattctc ttctgggtct
     7381 acgagcaaaa tccccatacg tccatgatct tctaaagctt ctttgattgg ataattcatt
     7441 ttacggatac gacaaaggat atctgccatt tcttctgtgc tagggataat caatgaaaaa
     7501 cggttcagct ttttgatcac ccccatgtga tcattcgctc taggactttc ttccagcagc
     7561 aacatttcac ttccagcttc ttctgttcct aaaatggcta attcgttttc ttctcttttc
     7621 aaatcaaagc caacaacgtt cttgtaaaaa tcaatcattt tatctcggtc tttcacccga
     7681 atggctacac tttttaaata ggtggcagcc cccaattgaa aatctttcat atttcttgct
     7741 cccctctacg tcaagcttta ttttaacgat ggattcttct tctttcaagc aataactttg
     7801 attttgttaa aaaaaatgtg agaaaattcg cattttgctt gttttttgta aattaatgag
     7861 taaaaactct tataaa
//
LOCUS       DAYYAO010000110         7861 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000110 DAYYAO010000000
VERSION     DAYYAO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.115"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACHARI_002495"
     CDS             complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACHARI_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8443501.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACHARI_002496"
     CDS             complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACHARI_002496"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8443502.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACHARI_002497"
     CDS             complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACHARI_002497"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8443503.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5270..5475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5738..7039
                     /locus_tag="ACHARI_002498"
     CDS             5738..7039
                     /locus_tag="ACHARI_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8443504.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7108..7707)
                     /locus_tag="ACHARI_002499"
     CDS             complement(7108..7707)
                     /locus_tag="ACHARI_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8443505.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2339 a         1600 c         1374 g         2548 t
ORIGIN      
        1 aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga
       61 cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt
      121 gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa
      181 tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat
      241 cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg
      301 ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac
      361 gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc
      421 cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt
      481 tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc
      541 acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag
      601 cacaccaata aataccatga agctgaagat atttccgatc acttctggta agcctaggat
      661 ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac
      721 gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat
      781 aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact
      841 catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg
      901 ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag
      961 tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac
     1021 ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat
     1081 cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag
     1141 ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat
     1201 acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag
     1261 tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc
     1321 ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa
     1381 tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt
     1441 cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac
     1501 tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa
     1561 acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa
     1621 gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa
     1681 catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt
     1741 gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct
     1801 ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg
     1861 ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt
     1921 tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa
     1981 gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca
     2041 tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct
     2101 accatattga aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta
     2161 aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt
     2221 aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct
     2281 gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct
     2341 gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat gcggatatct
     2401 ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt
     2461 acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct
     2521 ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg
     2581 aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg
     2641 tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg
     2701 ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt
     2761 gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag
     2821 ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct
     2881 tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt
     2941 tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac
     3001 agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact
     3061 agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa
     3121 agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag
     3181 ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa
     3241 gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat
     3301 ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg
     3361 gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg
     3421 tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg
     3481 tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt
     3541 tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat
     3601 gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta
     3661 cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat
     3721 gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac
     3781 aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg
     3841 gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat
     3901 tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact
     3961 taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt
     4021 taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt
     4081 ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca
     4141 ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca
     4201 atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa
     4261 cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg
     4321 ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat
     4381 cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat
     4441 caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca
     4501 atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta
     4561 atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg
     4621 ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg
     4681 agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga
     4741 agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt
     4801 tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg
     4861 cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc
     4921 ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca
     4981 attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag
     5041 ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt
     5101 cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt
     5161 tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt gtaattaatt
     5221 atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga
     5281 aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta
     5341 acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg
     5401 tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc
     5461 gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca
     5521 acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt
     5581 ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca
     5641 atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg
     5701 gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat
     5761 tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat
     5821 ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa
     5881 agaaccgaca gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta
     5941 cgattatgac caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac
     6001 acaacagctt cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc
     6061 tactcagatc tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca
     6121 ctctcaacag gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc
     6181 gatcgaacaa ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa
     6241 agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt
     6301 ggttctttca caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag
     6361 ccgattggtc gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga
     6421 agaaatcggt gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga
     6481 tttttcttat cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg
     6541 ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga
     6601 agctaaaaaa gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg
     6661 gggtcacttg aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg
     6721 gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc
     6781 agacatcagc gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag
     6841 tcaaggtttc gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg
     6901 tacggatttc tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt
     6961 agacggaaaa aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga
     7021 agcacgcgaa tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt
     7081 tatcgtcagt ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag
     7141 gacttctgtc cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat
     7201 tccttctacc attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac
     7261 ttttccttct ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt
     7321 atacgcatcc atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa
     7381 atcgatataa aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc
     7441 aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa
     7501 atcattcata aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt
     7561 aaaaatcgtc gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc
     7621 ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc
     7681 ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata
     7741 atatatatag ttcaaattta ttctgaggtt tattataact atctattgag aaagtatcaa
     7801 gctagcaatt ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac
     7861 t
//
LOCUS       DAYYAO010000111         7700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000111 DAYYAO010000000
VERSION     DAYYAO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.116"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1345)
                     /locus_tag="ACHARI_002500"
     CDS             complement(203..1345)
                     /locus_tag="ACHARI_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443506.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(1350..1535)
                     /locus_tag="ACHARI_002501"
     CDS             complement(1350..1535)
                     /locus_tag="ACHARI_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8443507.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1889..2134)
                     /locus_tag="ACHARI_002502"
     CDS             complement(1889..2134)
                     /locus_tag="ACHARI_002502"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443508.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(2128..2529)
                     /locus_tag="ACHARI_002503"
     CDS             complement(2128..2529)
                     /locus_tag="ACHARI_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8443509.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2716..4524)
                     /gene="uidA"
                     /locus_tag="ACHARI_002504"
     CDS             complement(2716..4524)
                     /gene="uidA"
                     /locus_tag="ACHARI_002504"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8443510.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(4547..6313)
                     /locus_tag="ACHARI_002505"
     CDS             complement(4547..6313)
                     /locus_tag="ACHARI_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8443511.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(6326..7120)
                     /locus_tag="ACHARI_002506"
     CDS             complement(6326..7120)
                     /locus_tag="ACHARI_002506"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8443512.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(7130..>7700)
                     /locus_tag="ACHARI_002507"
     CDS             complement(7130..>7700)
                     /locus_tag="ACHARI_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8443513.1"
                     /translation="NDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFF
                     SAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDG
                     TVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFL
                     IVGLILFFLYPLSKDKVLENCELLKQKHK"
BASE COUNT         2455 a         1497 c          957 g         2791 t
ORIGIN      
        1 aaaaccaccg gtggcaaacc ggtggcaaat ccatgtaaac atggtttatt ttttacactg
       61 ttttcaaaca aacccttgct atttcttcgg ttcgtcctcg tccattttcg ataccgacag
      121 ctagcttata acccaaggta tatcaacata aaaaaataaa aataggctat gacagcagcg
      181 tgacagcaaa aaagatggaa agttaaatat caaacctcgt aatttcattt gcgagtgctt
      241 gatttgtttc tggatataag tgtccatatt tgtccaaagt agttttaata gaggcatgac
      301 ctagacgctt agatattgca tagatatttt tattatgttc aattaaaaag gcaacatgcg
      361 agtgtcttaa tccatgcata tgaattggtt ttactcctgc aatttcagct agttttttta
      421 ttctgcttaa aatagttctt ggagatggcg gtaatccatc aaaagaaaaa accaattcta
      481 tatttttccc aattgattgt tgagtttttt tccatgtttt taaatcttca acggtttgtc
      541 tgtctaacga aattatccga atacttgatg tattttttgt ttcagcaaat tcataatctt
      601 gtcttgtacg gatgtaaatt gacttattta cgctacatct gccatttatt aagtcaaagt
      661 ctttccattg taacgctaag agctcctcac tccttacacc tgtaacaaaa ataaaacgca
      721 tcaaacgttt ataaaattct tcataaaaat ccccgtgata aacactatta tatactttct
      781 taaattcatc aactgtccaa aattctactt gaggacgctg agtttttatt ttacctatag
      841 tatctacagg attttcttca ataatttcaa gtacaattgc ccttttaaaa actactctca
      901 acttattgaa tatctgttta atataatttg gtgataatgg ttgctcaatg ccctttttta
      961 caacatggat tttcgataaa aactgttgaa attcctgtat atgaattggt cgaatcttta
     1021 caacctgcat ttctccaaaa aaatccaatg cacgttgtaa aatatgatct gttttcatat
     1081 atgttttttc tacagttccc aacttatacc aaggaaaaaa atactctcga taaaaatcat
     1141 gaaacgttat tccttgcaga taaattcctt gtgaatcaga aaaactttgt tttaactctt
     1201 cgagtcccct tctagcttct ccttgagttt tgaagcctct tctggtcact tgaattcttt
     1261 ttccaaagcg atcttgtcct aaagttgcag aaaaaaacca agtgcctctt tttctgtctt
     1321 tataaacatt cgcaattttc ctcattttac tattcctccc aagaaatatg gagaacttct
     1381 tctaaaattg aaataggaat tctttttatt tttgaattac gataaaaaaa gtatcctttc
     1441 tcaactaaaa gttctttaca ttccttgaag attttattag catagtactg agaaaaacct
     1501 agattaatca aatcttttcg agtagctgtt ttcattatag caccttcctt actttttttc
     1561 atttatttct atcagctgct tttgtcaaaa gcacatagga atttcacctc tccccaagtt
     1621 tacggcgagc cttacggaag tatcattatc ccaactttaa atcatcgctt tttattgact
     1681 ataaaaatta ttttagaata tttatcgctc cttctttaca gcaagaaatc atggcgcaac
     1741 taaaatatgc tccttaaagt gttaacgtac tgatagaatc tatttaattt tcaatgaaca
     1801 ataggttttc tacctaaaag aatctactaa ttccttaact attagaagat tcttttaaac
     1861 agaaaaagtg gaaaggggat ttttttaatt agttaatttt aaattttaag atcgcttcta
     1921 ttaatttaat ttctagctga cttatcatat attcatctac ataggtaacg aattgaccta
     1981 cctcgttaaa tccatttgtc aaacataact tagccatata agaatgataa tgtttgagta
     2041 tcatcttgat cgaaattgga tctccttcta gagcagataa gataatggat agtggaacta
     2101 ataggtactg ccgcttttta gatttatcta ccataggtca taccccattt tcaattgttc
     2161 aactgccttc tttcgttgat accagatacc acttgtgctc atattgagaa ttttaccgat
     2221 ttcagcatca ttaaaacctg caaaataata aagtaggata atctttcttc ttatctccga
     2281 taactgatca attgctgctg ataacttttg atctgaaatt aatatttcca ttccaaataa
     2341 gaaatagaca tcttgttcat caaaatgagt ttgggaatac ctgccttcat ttgaatctgc
     2401 caatggttcc tcattatcct ttatgttagc gtttcgtttt cgaatagact tagcttcgtt
     2461 tcgtaatact tttttacagt aggcatcaaa ttggtgttgt atagatatgt cattgtaatg
     2521 ttgttgcatg ataatatgct cctttttccc cctttccact aatacaacaa tcagaaagcc
     2581 cctttttttc gaaaaaacta gaaaaaaaac ataaaaatat ttatcattct ttaaacagaa
     2641 aatctttcta taaaaaacaa aaaaaaacga ttaggctagg ctaatcgttt tttgaacatt
     2701 cttaaaaaat ccaaattatt ttttataact gaaattgggt attgatgacc atcgtttctg
     2761 tagatagtaa gcaatcattt taggttttct atctctagta aagattcctt tcttatttcc
     2821 ttgcactctt aaaatccctt gacttgttgc aaaatctgca aaattccaca cttgttcacc
     2881 aacaaaattt tcacatgtat ctattacttc gtgatttgct tcgtaataag ccacttgata
     2941 ctcttctgta aacattgttg gtactgtatc atgaaatcca gctactgtat ctgctccata
     3001 ttctgtaaac attattggcg tatcaggaca tctttccatc catccatcta gctctttttt
     3061 caacagatat tttgcatgtg ccagatcccc tcccgaaaca taccaaccat aataacgatt
     3121 taaacataaa acatcgacaa tatctcctac cttacaaaca tctggcgttg attttagata
     3181 agtaacgatt gttacaggtc ttttctgcgg atcaagttct ttagttaatt caactaaagg
     3241 ttcaaaatat tctttagcac cttcggaatc cgaatctggt tcatttgcaa tagaccacat
     3301 tacaacactt gggtggttct tgtcgcgttc tatcaattcc ttgatcaccg ttttatgaga
     3361 attttctgtt cgtatctctt tccacgtatt atgtttttcc acattatcat ctaataaagt
     3421 ggccatgaaa tttaaatgta accctactgc aggtacttca tcaataacta caataccttc
     3481 tcgatctgct aatcgcataa tttcttctga gtaaggataa tgtgatgttc taaaagaatt
     3541 agcacctatc catttcatta aattaaaatc tttaatattt acagcctcat tcaaaccacg
     3601 tccattaaca tacgaatcct catgcttacc aaatccttta aaataaaaag gtttattatt
     3661 aatcaaaaat ttgccattgg taacctgaac actgcgaata ccaaattctt ctgtataggt
     3721 atcgatcact ttattatcat caattaaact aattttcata ctatataaat aagcgtctag
     3781 aggttcccaa aaaattggat tcttaagctg aatcattcct ttctttcctt cagcttcagc
     3841 tacaagttga gcctcttcgt catatatcgc tattaagatt tttccggtcc attctgtatc
     3901 gatattgaat tctacacttg catccaaatc agttattctt gtattaatag taatatcact
     3961 tatatagtct ttcggagttg tatataactt aacaggtcta tgaatacctg cataattgaa
     4021 gaaatcaaaa ttaactaagt tttccttttt tatatcccca ttttcctgta ctgtttcctt
     4081 cataatcccc acaggtaaag tactatcgtc aataatattg tttacagcaa ctgtaattcg
     4141 attattcccg tcttttacaa accgattaat ttcagactca aatggtgtaa acccaccttt
     4201 gtgttgagta ataaactttc cattcaaaaa aacttttgct tcatgtgttg cagatccaaa
     4261 acgaagaact aatctttctt ctaataatct tttaggaaga gtcacttctt tttcatacca
     4321 cacccatcca acatgatttc taatgttctc tgtttcaaat aaatcattat aagaagaggg
     4381 aactgaaatc ggataactct ctttttcatt tattggttct aaagcaagtt cttcctgtaa
     4441 gtcatcattt gtatctaatt taaatctcca gagaccgctt aagtcaatta cttgcctcgt
     4501 ctctgtcata atgggataca acatataaac cactcctatc taaatattaa ataaatgtat
     4561 cctttaaacc acaataagca tctattggac ttactccctt aaattcagaa agacccatta
     4621 gtttgtccac caatatttct aaagtttctt gttttccatc ataagtatta atataagtct
     4681 ttacttgagg tacatcagcc aaatgaaacg gacactgtac agatacaaaa attgttggaa
     4741 tttcatgaat gtaaaacggt atatccggag ttcctttact tgctggccag acaattcgtt
     4801 gaactgtgcc tggattaaca ttagctatat taatgatcaa gtcatatttt ttagttaaat
     4861 ttttaattgg tctcttagca gcatatattc cagctatatt tttaattttt tcttcttgcg
     4921 ataatccttt caattcttct tcaggtgaaa tgtatttttc aacttcaaat ccttttgctg
     4981 acaaaatctg tttcaataaa tcaatggctt gaggtctatt tccagcaact acttcaccaa
     5041 acccaccttt taccccatgt acatctacta gtaaaatcct tttatatttt tctggtacaa
     5101 taggccaaat atcttgttta tctttaacta atgtaattgc ttggtcagcc acctctttaa
     5161 aaattgattt attttcaatt aaaccaattt tttccagagc ttcttgctta ggtaataaaa
     5221 tttgttcttt tgcttttttg tgtaatccca atttagcttt taaccctaaa attcttttta
     5281 aggcatctaa taaccgatca tcagtgataa ttcctttacg atagccttcc atcatataat
     5341 taaagtcttc atctggatca ttaaaaaata gaaatagatc acatccagct gcaatagaag
     5401 tgggcaataa ttctgatcgt ttcattgccc cagtcattcc taacatatga ctagcatcag
     5461 taactactaa tccattaaat ccgagttttc ctctaagtaa gtcagtgatt aattcttttg
     5521 ataaagttgc aggaagtaaa tcttcatctt tcagatcagg attaaaatgt tttgaataag
     5581 ctggcatatg gatatgacct gccataattg aaggtaatcc agcttctatt aagcctgagt
     5641 atacttttcc aaaagaatta tcccattctt ccgtacctaa actattaata ctaaaagata
     5701 gatgctgatc tcgttcgtca ataccgtcac cagggaaatg tttagctgca ggcaaaattc
     5761 cactctcctg aatacctttc atataagcta aactaagttc taaaacttta tcagggtttt
     5821 gtccaaaaga tcgactagaa atgataggat ttctccaatt tctattaata tctacaatag
     5881 gagcaaaact ccagttacat ccaatagccg atgcttcaat cccagaaact ctccctaatt
     5941 cataagcata tttcgcatca tttgttgcac caatttttac ttctacacca acttcagtac
     6001 catctgtaca agctccgtta ccacctgctt ccgtattagc agcaattaat agaggaattt
     6061 tactttctgt ttgtaagata tagttttgat catatacctc atcagcagtt cctggattat
     6121 atcgtacagc tccaatatgg tactgattga ccatattact caagtaattt ttatcacgac
     6181 tagatcccat attaacaaac aattgaccta ttttttcttc caaagtcata gaatcaatcg
     6241 tctcttcaat ccaagaaata gcatcttcat ctaaattata tggttttttt cgtaaatcta
     6301 cttttatagt cattttcttt ctcctctact tattagtaag atttttccaa aaagtatcta
     6361 tatatttttg ttcaatatct acataatctc ccgataaaca tttaaataaa acatcagttc
     6421 ttaagttttc ccatgattct ttttctttat tgaccaaaat aggataaaag aaaactttat
     6481 ttttatttgc agcttctaga tcaccaggag aatcaccaat cattaatatc ttatccaaat
     6541 cacaaccatt ttcaattaca cttcgaataa tatcttcttt ttttccttta tcttgacaac
     6601 acagttcatc aactaatgat aataattcat gtctttccca ttcttcttga acagcttcct
     6661 tattagctga gcttacaata attatcttcc catatttttt cagagctttt atagtatctc
     6721 tagcacttaa aaagggttta tctaatcctt ttgcttgact aatctgttga ttgacttctt
     6781 ccgaccattt taaagctttt aataaatcct ctgactgaga atattctatt tccctcttca
     6841 aagatggatt agacaattct tttgttagag atacccagtg ctttaatcga ttaatcgatg
     6901 ttacatcatt tttttctaat gttttaacta aacctaaaaa tctgtttatt ccacgagtct
     6961 ctgaatataa attaatatta ttccaatcct gtaggaattt ttctctatga tgaacatcaa
     7021 aaatctctgc agcaattggt ccgaaaaaga gattgtgttt ataagtcatc gtatccataa
     7081 cacagccatc tgaatcaata caaaatatcc aattattcat atttctctcc tatttatgtt
     7141 tttgttttaa aagctcgcaa ttttctaaga ctttatcttt agataatgga taaaggaaaa
     7201 ataaaattaa tcctacaatc agaaaacaaa ttgatggaat taatgttgaa gcgttatata
     7261 acttttctaa aacatctccc gtttgtacat ttgctaaaga gtcatagcct ataatagaaa
     7321 gagtccaacc tccgacgcct ccagctagag cttgacctat ttttctagca aaagaatata
     7381 ccgcatatac ggtcccatct tctctttgat cagactttac ttcttgatca tcaataacat
     7441 cagtaatatt tgcccaaata acagtattaa acacacccaa acccatatag ccaaccgaac
     7501 taagaattaa gaatacatac atattactag cttttatcaa aaataagatc aaatatacta
     7561 ttgctgaaaa gaacattcct actgatgcca gttctttttt tccatatttt ttacttaaaa
     7621 acaaagttaa aggcgaaaca actattaaaa ccaaaatagg attgataaaa ttcattaaaa
     7681 ctattccctg tgcatcatta
//
LOCUS       DAYYAO010000112         7348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000112 DAYYAO010000000
VERSION     DAYYAO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.117"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            366..635
                     /gene="rpsN"
                     /locus_tag="ACHARI_002508"
     CDS             366..635
                     /gene="rpsN"
                     /locus_tag="ACHARI_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8443514.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            650..829
                     /gene="rpmF"
                     /locus_tag="ACHARI_002509"
     CDS             650..829
                     /gene="rpmF"
                     /locus_tag="ACHARI_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8443515.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            861..1238
                     /locus_tag="ACHARI_002510"
     CDS             861..1238
                     /locus_tag="ACHARI_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443516.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1259..1408
                     /gene="rpmG"
                     /locus_tag="ACHARI_002511"
     CDS             1259..1408
                     /gene="rpmG"
                     /locus_tag="ACHARI_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8443517.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1432..2415
                     /locus_tag="ACHARI_002512"
     CDS             1432..2415
                     /locus_tag="ACHARI_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8443518.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2445..2567
                     /locus_tag="ACHARI_002513"
     CDS             2445..2567
                     /locus_tag="ACHARI_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8443519.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2585..4108
                     /locus_tag="ACHARI_002514"
     CDS             2585..4108
                     /locus_tag="ACHARI_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8443520.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4132..4371
                     /locus_tag="ACHARI_002515"
     CDS             4132..4371
                     /locus_tag="ACHARI_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443521.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4564..5127)
                     /locus_tag="ACHARI_002516"
     CDS             complement(4564..5127)
                     /locus_tag="ACHARI_002516"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8443522.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5220..5438)
                     /locus_tag="ACHARI_002517"
     CDS             complement(5220..5438)
                     /locus_tag="ACHARI_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443523.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5627..6574
                     /locus_tag="ACHARI_002518"
     CDS             5627..6574
                     /locus_tag="ACHARI_002518"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8443524.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6541..6630
                     /locus_tag="ACHARI_002519"
                     /pseudo
     CDS             6541..6630
                     /locus_tag="ACHARI_002519"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6763..7170
                     /locus_tag="ACHARI_002520"
     CDS             6763..7170
                     /locus_tag="ACHARI_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8443525.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2600 a         1090 c         1438 g         2220 t
ORIGIN      
        1 taatcaaagt tttaaaaaat tggcgataaa ccagttaaac ataaaagatt gaataaaaag
       61 ttatagtttc tttcatattg ccgttttaag tagaggaaaa gctacttaaa acggtatttg
      121 tgtttgattg gactaataaa agtgaattct tatttttcag agcttaaact gcatattctt
      181 ttaaatgatt tgattatctc tatgtaccga taaattcgtt ttcaaatagt tatctattca
      241 tatgagtttt atttcatgtt atttattgtt gacaagagca actcttgttg gtaaagtatc
      301 agtgtcaaaa cgtaacgatt acgatttgag atgtctaaat caaaataaaa ggagtataaa
      361 ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa acaaatggcg atgattgaaa
      421 agtacgcaga taaacgtcag gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat
      481 tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa
      541 gagggtatat gcgaaagttc ggtatgtccc gtattaagtt tagagaactt gcccatcaag
      601 ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc
      661 agcaagaaaa acatctaagg cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa
      721 accagaaatt tcatttgacg aagcgaatgg tgattataga cgtagccatc atgtatcttt
      781 aaaagggtac tataatggaa agaaagtaat taaaggagct tcaaaatagt ttaatatata
      841 acttttagga gggaatcaaa atggaaatta tttacccacc tttagttgaa gaaggattaa
      901 agtattatct tgaaacgacc cagcaatcgc tagataaatg cactttttat cgttcgatgg
      961 tagagagagg aattattact gaaacgggtt taccgaccca acaggcaatt gaaaatggat
     1021 tagtcaaaga ttactatgag gaccaaggct tatcatttga tgatttttta agaatctatc
     1081 caatttttga agagtatgac gaggagttat ttcaatgtat tgatggttat tgggagatac
     1141 ctgttgatat gaaagaaaat ttagtttcac aattggaatc tggcgagtta acttttgaag
     1201 atgcacaaca aattcaagca tatcttgaag atcgataagt attgaaggag gaagatttat
     1261 gcgtcaaaat attattttgg aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa
     1321 aaataaacgg aataaccctg aacggttaga attaaaaaag tattcgccaa aattgcgcag
     1381 acgggcaatt ttcaaagaag tgaaatagct aagtggtaaa aaggtgacaa aatgggcata
     1441 ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa caacgctaat caatcaagcc
     1501 ttgcaagtct caccctaccc cagggaagaa attattataa ttgaaaatga gtttggtgaa
     1561 gttgggattg atcacaaatt attagttcat agcgaggaac gggtgttaca actaaataat
     1621 ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa tattgatttc tttactggaa
     1681 acaatagaag aacatcaaca accaatttca caaatcatca ttgaaactac aggtatagca
     1741 gatccccaac caatcattca aacactttta acagcgcctc aactaagagg aagagtatat
     1801 atcgatagtt tattgactgt tattgatagt gacagttttg aacttattga aagagaacca
     1861 caagccctta agcaacttgt catagcagat cgcatttttg tgtctagaaa acaaaacttg
     1921 aatttatctg cactaaaaaa attacagaga gaaatcaaaa gtattaaccc tttatccgat
     1981 attcgaatgt tcacacatag tgaaacgatt caaaaaaagg atttttataa tttggaaaaa
     2041 ttcaatcatc ccttagtttt tgaaagtgat tcatcagaaa atcactataa tggcaaacac
     2101 tatcatcaac atcatcaaga acatgagttt aaatcaattc tcttaacttc agatcaagac
     2161 ataaaaaaag attttttatt ccaattaatt gaccggctga ttttaaccaa ccaagggagc
     2221 ctgtatcgtt ttaaaggatt gattgctttg caagatcaag attttatagt tgccatacaa
     2281 ggggtaaacg aacaggttaa ttttcaatac accactcgac aaaaagacca aaccactatt
     2341 attctaattg gaaaaatgtt ggatgaagaa aggatcaaac agagttttga taaattactg
     2401 gcggaaagca aataatgata agtattgatt aggggtaatt tctaatggag aaaacggata
     2461 tatctagtgc atatcgacga cttaaaagcc cgaatatcaa aactagaaaa agggcattaa
     2521 aaattatcaa agaacataaa caaaataaaa tgaagaaatt agcataatta aggaaggaaa
     2581 aagaatgaag aaaatcagtg taggacttat tgggatagca gctctaggat tattgggagc
     2641 ttgctccagc actaatgatg caaaagtatc gaatgataaa gatggaaaat tagaaattgt
     2701 aacaacattc tatccaatgt atgatttcac caaaaacatc gtcggcgatg aagcgaacgt
     2761 ggatttaatg gttccagcgg gatcagaacc tcatgactat gagccctcag ctaaggatat
     2821 ggcaaaagct catgatgctg atgtttttgt atatcataac gaaaacatgg aatcatgggt
     2881 accaaaagct aaagaaagtt ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga
     2941 catgatttta ttgccaggta gtgaagaaga agatcatgat cacggagaag aagatcacca
     3001 tcatgaacta gatcctcata cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa
     3061 tatcaaggat cagttagtta aattgtatcc gaaaaaagca aaagtatttg agacaaatgc
     3121 tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac tataccacta gtcttaaaga
     3181 agcgaaacaa aaaagttttg tgacacaaca tgcagccttt ggatatttag ctttggatta
     3241 tggattgata caagttccta ttgctggact aagtcctgaa gaagagccat cttctggtcg
     3301 attagctgaa ttaaaagagt atgttaaaaa aaataagatc aattatattt actttgaaaa
     3361 gaatgctaat gataaaatcg caaaaacttt ggccaatgaa gctgggataa aattggaagt
     3421 attgaatcct cttgaaagtc ttacaaaaga acaaatggat aatggtgaag attatgtttc
     3481 agtaatggaa gacaatctaa aagcgttaga gaaaacaact atggttgctg gtgaagaggt
     3541 agttccagaa aaagaagcga aggatgaaaa gacagttgct aatgggtact ttaaagatgc
     3601 cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa tggcaatctg tttatccgtt
     3661 gttaaaagat ggaattttgg atgaagtttt cgattacaaa gcaaaactca ataaagacat
     3721 gacagctgcc gagtacaaag attattatac tactggctat aagacagata ttgatactat
     3781 caacattaaa gacaacacca ttgattttgt agtcaacggt gaacatcatc aatacacata
     3841 caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa
     3901 ttttgaaact gatgatgcag gtgctggtcg ttttaaatat attcaattta gcgatcatgg
     3961 aattgcacca agtaaggcag agcatttcca tattttcttt ggtggagaaa gtcaagaaaa
     4021 actttataat gaaatgcata attggcctac tttctatcca gcttcattat ctgagcatga
     4081 gattgctcaa gaaatgatgg cccattaaaa tccaatcttt ggaggaaata catggtaaga
     4141 aaagtattat tgtctatgga ttccaagaat attgatgctt atcggcgtta tcaatttgca
     4201 aagcaaatga gattaaatgg gagtacatta gctgatattg ctgcaacttt acatgttagt
     4261 attgacacag ttcatagagt attaaaacgg gatcctgatt gttgttttat caaggtcaat
     4321 actggaagaa gtagacgttt agcaaaacgg cataatagct tggccaatta attgataata
     4381 ccagagtaga tcatttttcc cttatttgga tttgatctgc tttttattat ttcttgaatt
     4441 gtcaaatcaa gcgcaacact ttatcaaaga agacttcata aggtgttctc caatctaaac
     4501 actttctagg tctgttattc agtttttcaa tcatctgatt tatcgttgag ttatctaccg
     4561 tagctatctc tttcgatttt ggcaagtatt ctcgaatgag tctattcgta ttttaatttg
     4621 ttcattgttg ccaagggcat gaggatctgg aaaataaaag ggcatattat tcaatacttc
     4681 tgtaactgcg aaatgattgg agaattcttt tcctctatct ggagtgatcg ttttgaccgc
     4741 ctcatttgga atactgctta aaagttcaat cattttctcg ctcacataag cggctttctt
     4801 tctggggacc ttctttcctg ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa
     4861 acaagtacca ctagttttgc cagctacaat atcggctttc caatgaccaa aatcacttct
     4921 atcatctgcg gattttggat ggccatggat cgaatgagta aggcaaatct tcccacgaga
     4981 ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc
     5041 tttattgccg tgtgagaggg gttctgtatc gaaaagatca ttgcaaattg cacggtagat
     5101 cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc aaacgattcg agattttctc
     5161 tggcgaccac tgaaaatcga caaataaatg acgaacaatt gaccaaactc acggatcatt
     5221 tagaatgtga taacgtccgc atttttttct tcgataagca taattttttt ccgctttgga
     5281 aggtgattag tttttttctt gtttcttctc agttcatgta aaatagtaaa cgggctatgc
     5341 tgaatgtcgt tgccaattat actaatagaa aatccttctt cgtgaagcag aaagattctt
     5401 tctcgtttga gtatagtaag atgacaatac tggctcataa ttattttcct tctaggagtg
     5461 ttctcgacaa acttatacta gttggatatc gattatgact catttttatg tgttgcactt
     5521 aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa aaaataccta ttacaatctt
     5581 aactggctat cttggttcag gaaaaacgat acttctaaat catattctgt acggtgatca
     5641 cggaaaggaa attgctgtca ttgtaaatga cataggggaa gtaaatgttg atggggtatt
     5701 agtcgacaag aaaggtttta aaagaactgg agaaaaactt gtttcgatgc cgaatggatg
     5761 catttgttgt acattacgcg aagatttgat tgttgaacta gatcaattag tgcaattttc
     5821 tgacttgaat caaattgtta ttgaagcttc aggaatcagc aaaccaattc caattattca
     5881 agcaattata ttagctaaaa accctttaga cattgatttg acccaacgat tagagattgg
     5941 tgccattatt atagtagtgg atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa
     6001 gattcaggaa agaaaagctg aaggtaatgt agttgaagaa gaacaggata ttcgtggctt
     6061 gttaattgat caattcgagt gttgtaacat attactttta aataatagtg acttagtgtc
     6121 aaccaagacg atttacaaaa ttaacggtct aattgctaag ttacagtcca atgccaaaat
     6181 aattaggaca attaaaggtg aagtagactt atcgaaaatt ctaaatacaa atctttttaa
     6241 tatagatgag acttcctatt caatgggctg gttcaaagag ttagagcttg gctatcaaaa
     6301 tcatattcct gaaacagaag aatatggtat ctcaagtttt atctatcgaa atcggttact
     6361 ccttgacgca caaaaatttg cccatcttac ggataaaaat tttcctggca atattacaag
     6421 agcaaaaggg tttctttggt ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc
     6481 gggtagccat aaaagtattg aatcattaaa ttattgggta gctagtttac ctaaaattga
     6541 gtgtcaaatg attatacaaa aaaacccgaa ttgaaggaag cctggcatcc gttgtttggt
     6601 gaacgcatta ataaactagt ttttattggt atgaagatgg atcaacaatg tattaaggaa
     6661 caacttgagg gttgtttaac tgattctctt aatgcagata atttccgaaa aatttcacca
     6721 tttccatgga aaaattctaa tatgtttagc cagatatcgt taatgaaatt tttaagaaaa
     6781 gctggtgtca agataggcga tgtcataatt attggcattt tgattattcc ctcttttatt
     6841 cccttatttg tattctctat agaaaaggag aatgaaacaa ctgaagatgc tatcaaatat
     6901 gccatcgttc aaattgatgg caaggaaatg gatcgcttcg atttggataa gatcaatcat
     6961 aaactcgtga attatcatcc tgcgaaggga caatataata ttgttgagat taaagatgga
     7021 agaattcgag ttaaagaaga taatagtcct gatcaaatcg cggttagaac tggatggatc
     7081 tcaaaacccg gtcaaacgag tatttgttta ccccataagt tagttattag tattgagaaa
     7141 aaagaaagca aagattacta catttactaa tctggaaata gatgaggagg tttttaataa
     7201 gaaaaagata ttattattaa taatagcagg ggctttatta agtgtgttaa gcgcttgtgg
     7261 acaagaaaaa gaagcggtga caacaggcac aagtatcttt aacgggacct gcaatggttt
     7321 agttaacagt cggttagatt acttattt
//
LOCUS       DAYYAO010000113         6907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000113 DAYYAO010000000
VERSION     DAYYAO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.118"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHARI_002521"
     CDS             complement(<1..182)
                     /locus_tag="ACHARI_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8443526.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHARI_002522"
     CDS             517..951
                     /locus_tag="ACHARI_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8443527.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHARI_002523"
     CDS             974..1294
                     /locus_tag="ACHARI_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443528.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHARI_002524"
     CDS             1435..1626
                     /locus_tag="ACHARI_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443529.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHARI_002525"
     CDS             complement(1697..1954)
                     /locus_tag="ACHARI_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8443530.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHARI_002526"
     CDS             2112..2375
                     /locus_tag="ACHARI_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443531.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHARI_002527"
     CDS             2399..2587
                     /locus_tag="ACHARI_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443532.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHARI_002528"
     CDS             complement(2670..2855)
                     /locus_tag="ACHARI_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443533.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHARI_002529"
     CDS             3070..3441
                     /locus_tag="ACHARI_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8443534.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHARI_002530"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHARI_002530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHARI_002531"
     CDS             complement(3709..4137)
                     /locus_tag="ACHARI_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443535.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHARI_002532"
     CDS             complement(4197..4955)
                     /locus_tag="ACHARI_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8443536.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHARI_002533"
     CDS             5250..5447
                     /locus_tag="ACHARI_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443537.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHARI_002534"
     CDS             5553..6035
                     /locus_tag="ACHARI_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443538.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHARI_002535"
     CDS             complement(6093..6689)
                     /locus_tag="ACHARI_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8443539.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2340 a         1061 c         1356 g         2150 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     6901 aacagct
//
LOCUS       DAYYAO010000114         6822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000114 DAYYAO010000000
VERSION     DAYYAO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.119"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..546
                     /locus_tag="ACHARI_002536"
     CDS             190..546
                     /locus_tag="ACHARI_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8443540.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            556..1455
                     /locus_tag="ACHARI_002537"
     CDS             556..1455
                     /locus_tag="ACHARI_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8443541.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1975..2703
                     /locus_tag="ACHARI_002538"
     CDS             1975..2703
                     /locus_tag="ACHARI_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8443542.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2848..4875
                     /locus_tag="ACHARI_002539"
     CDS             2848..4875
                     /locus_tag="ACHARI_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8443543.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            4983..6080
                     /locus_tag="ACHARI_002540"
     CDS             4983..6080
                     /locus_tag="ACHARI_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8443544.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6387..>6822
                     /locus_tag="ACHARI_002541"
     CDS             6387..>6822
                     /locus_tag="ACHARI_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443545.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT         2394 a         1102 c         1384 g         1942 t
ORIGIN      
        1 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
       61 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
      121 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
      181 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
      241 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
      301 caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa agatacacaa
      361 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
      421 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
      481 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
      541 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaat
      601 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
      661 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
      721 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
      781 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
      841 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
      901 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
      961 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     1021 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     1081 cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa caaattaaat
     1141 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     1201 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     1261 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     1321 gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg tgcccatcac
     1381 ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca agcgatgaca
     1441 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     1501 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gcggaaaata
     1561 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     1621 gtattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt ttatttaatt
     1681 ataattaata ttgattgaag tctaggcagt caagtcacat tttgatagat aggaggggga
     1741 cttacgaatt attttaaagg ctatgacagc tgtatttcta gattgtgttt gcatcaagtt
     1801 gcaataaaaa ctagaaatca aatcgggctg ataatcattt ataactaatc gatacatagg
     1861 accaaatata ggtctatgtt cttttatttc ataagtatcg taaaacagaa ttttgctatg
     1921 gattaatcca atcgactgat atgtttagta aataaagaaa gaggaatgtc aatgatgaaa
     1981 aaagaaatcg tattaacagc agcaatgatg ttattcagca tggtagcaag tacgactttt
     2041 gtttctgcaa ctgaagtata cccaaaggaa tataacacag aaggaacgat tacatttgaa
     2101 gcaggagatg agggtgtaac tcctcctgtt gatccagaga atcctgatcc caataaacct
     2161 gtcgatccat cagatcctcc aagccctgga acaggagggg cattatcgat tgattatgga
     2221 tcaaaattca aatttgggac tcaaaaaatt tctactgcgg ataaaacgta ttatgcagct
     2281 gctgatgtga tgaacgatgg gtctagaaaa ccgacatatg tccaagtgac agataggaga
     2341 agtaccttat ctgggtggaa attaagtgtc tctcaaccag aacagtttaa aactgccagt
     2401 ggggatgaac tagttggagc acagctgaaa tttacgaaag ggcaggctgt ttctttggtt
     2461 gatccaacat atacgccgca aacggtgaat tctgaattaa cgttgacacc gggaggaaac
     2521 aatacgttag caattaatgc taaatcagga acaggtgtgg ggacttgggt ctatcgattt
     2581 ggtgcaaatg aaaatgaaaa tcaagatgcg gtacagcttt cggtacccgg taaatctgtc
     2641 aagttagcgc aacaatattc cactaaatta gtttggacat tagaagatac acctaacaac
     2701 tagtttactg aaaagaactg ttaaaaataa atacgtagtc atattgtctt gcgaggtaat
     2761 cgagcaaaaa ttgtcaaagg ctaggctaaa tttgtttgga tacgaatagt tcatctagga
     2821 atttaacgat caggaggaaa agatatattg aaaaagagga ggatcgtgct cattttaagc
     2881 agtctcttag tgactaatat ctttccgatc atagggtact ctttggaatt gaatcagttg
     2941 gagcatggaa atagaagaga cgaagaaagc aaactcactt cgacattcga cagtttaata
     3001 gaatccaacc agacacaaga aagcagtgag gatttaagta tttcgagtag tggagagacc
     3061 actgacaacg tagaagctga gggttcggac aaacaaggac tagaagatgc accaatttct
     3121 gcggttgagc tgcctgacga tcccaatatt atttctatcg ataaaatttt tcaaacacct
     3181 atcggtacta gcacgtccat attggaaaat gggaagttac tgcagttgaa cccagcagaa
     3241 aggtctcaaa gaggagctat ttggtcaaat aagaagatta gtttactctc ggattttaca
     3301 tttaaatctt atttatactt aggaaatcaa cgagatagag cgggagatgg catgaccttt
     3361 acattgactg gcgatcctag aatggctagt aatcccagtc aagttattgg atcgccaggt
     3421 atgggaattg gctcatattc tactcgttct ggccaacctt acattgcgaa tgcgctctcg
     3481 attgagtttg atacgtataa aaatagtggt tcttctgatc gaatggatcg agagataact
     3541 gctgataata gagggcatgg acatgtcgct tttgtcacgc ctaaaccaaa taataataat
     3601 tattctggag agcatactgg cgtgacggta gctcctacat ttctttctaa tggcacatgg
     3661 cgcttgttaa taattaaatg ggacgcggag caaagaagtc ttacttatga tttggaaggt
     3721 gtcggtcgct cttctcggat tatacctgat ctcattaggc agtttggttc tacagaagta
     3781 aattgggggt ttacttcttc aacaggtact ttttttcaag aaaatgctgt cgctgtcacg
     3841 cgacttccat cgaatgtcac atcaacagct gatgtaaaag tcaacgatag tgattatggg
     3901 gccatcgctg aagctggagg aaacgataag ataactatca gaaatacatt gaaggtcgag
     3961 ggggattttc ttgaatataa gaaaatgcag actagtatcg acctcccaca agaattaact
     4021 attcccgagc atatcttgat caatggaaat caaattgatg ccaaagatat cttgataacc
     4081 ggaaatcgag tcacgctaaa tctgacagat tattttattg ctggagatac gttaataatc
     4141 gaattggaga caactttgca ggaagtaatt cctgaaaaag tactcactat gcattttgaa
     4201 taccttgaaa acgggaattt gattgagcaa tcgaatgaaa taaacctaaa gatcgcaaag
     4261 ctgaaggaga tagatatcca tgtttattat aaagataaag acagtcagat tgatattgct
     4321 gaaatgaaaa caatcactgg gaaaatagga gagaagtata aagaaactcc tattgatatt
     4381 gaagggtatg tttttgactc ggattcagcg aatacagaag gcatcttctc cgaaaataca
     4441 gaggatatct atttttatta cagaaaaggc aaactttatc tctcaactgc tcctcaatat
     4501 gtcaattttg gaaaacataa aatatccagt agaccattaa gtgaattcgg acagttttca
     4561 ggtgggttga aaattataga tgaacgggct tttggaagtt ggaaattaca attgaaacaa
     4621 gagaaagttc ttacaaatgg agattttgag cttccagaag ctctttcctt tgttacctct
     4681 gaaaatacaa cggttatagg gacatccgct gttacgattt tcgaaagtga tcaaaagggt
     4741 gaaagcgatt tgtcctctct tttagattct acagctcatc gtggaatcag aataaatatt
     4801 cctgtagaaa accaaagaat cggaaccttt gaggggaaac tatcttggat acttgcagat
     4861 gtcccaggaa attaacagga agagaagaat ataacaaaat tattgagttt caagaagggc
     4921 acaggtacca taggctcttg tgtcccacag agacaagatt ttgttatagt cttctcttac
     4981 atatgattaa aggagcgatt ttcttgagac caaaacaaca attatttata acactcctag
     5041 ttattttagg ggcacttttt aacaatatat cagtgtcagc tagtgcattt aatttttcag
     5101 tgacgccgat aacttcagag aatcaaatcg acaagagaaa aacttacttc gatcttcaac
     5161 ttgatccgga tcaggaagta gaagtaaaag cggaattgag aaatgataca gaaaaagaag
     5221 tgaaaatcga tatttctgtg aatagcgcaa ccaccaattc aaatgtgatg gttgaatacg
     5281 gaaaaaacga gattgaaaaa gacatgagcc tgatctttga tctggtagat tatgtgagct
     5341 atccacagat agtgacgcta aaacctaaaa gtgtccaaac cgttgctttt catgtcagga
     5401 tgccgaatga gcgatttgat ggtgtattgg ctggtgggat cacattccaa gaaatccaga
     5461 cggaaaagga ccaaacagaa actaaggatc aaagcttatc gattgagaat gaatacacct
     5521 atattattgc tttgttgatg caacagacac tgaacgaagt tgctccgaac cttactcttc
     5581 atgaagttaa accagatcaa atcaatgcaa gaaatgtcat cttagcaaac gttcaaaatg
     5641 atcaaaaaac ctatatcaat caagtagtta ttgaaacaaa aattacaaaa aaaggacatt
     5701 ctgaagtttt atatcaagag gaaaaagaag gattgcagat tgcgccgaat acaaattttt
     5761 cttttccaac tgctttaaat ggtcaaccgc taaccccagg tgaatatcac cttacaatga
     5821 ctgttttagg aaatgaaaat gaaaatggaa aattttcgag aaaaaaagga aatactacga
     5881 tcaactatac caatcaatgg gtatttgaaa aagattttac aatagacgga aaagtagcca
     5941 aggaattgaa cacgacggat gttacgatta aaaaagacaa taattggatt tatatcttag
     6001 tagcttcact tttcttattg tttattttat tatgcatact tttgtttgtt ttgagaaaaa
     6061 aagaagagaa agaaaagtaa ggttaagata acaaatagta aatttttatt attgtaggat
     6121 ggttgggctc tttttataac aatataaaat ataccatttt attaataaaa atgcatacta
     6181 gtaaatgata taattctaat aacaggtttg atcgcgagaa aggccaaata aaacgctctc
     6241 caaaatgtta aaaaaaagac aggacgatgt atattataca aacaagaatc gctgacaaca
     6301 gtgactcgga tccaggaaat cagttttatc cccagctagt tggtgaatgc atggaaagta
     6361 atatacgtag aaagagctga tgactaatga attatgagtt ttttttaagc aaagaagatc
     6421 acagaagtta ttcattactt aaatatttag aagcaagctc aaatttatca gagtcaatat
     6481 cgaatgttca agaagagttg tcgctatcga catttctctt gaaaaaaacg atcgataagc
     6541 tgaaacatga tttacaaaag ttcgatctag agaaaaattt gcgagtaacc gtcagtgaat
     6601 tggatatatg tctggaaatt aatggaaaat gttctagcaa agctctgtta tctcgttata
     6661 taacggattc tttatctatg aaaatggtat tggctttctt tcaggaaaaa tatatctcag
     6721 tagaaaattt tgctaaacag catcacgtga gttattctgt ggcttataaa gtattacaaa
     6781 gactaaaaag aaatctaaaa aaatatcaaa ttttttttga aa
//
LOCUS       DAYYAO010000115         6760 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000115 DAYYAO010000000
VERSION     DAYYAO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.120"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..667)
                     /locus_tag="ACHARI_002542"
     CDS             complement(143..667)
                     /locus_tag="ACHARI_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8443546.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(660..1133)
                     /gene="tsaE"
                     /locus_tag="ACHARI_002543"
     CDS             complement(660..1133)
                     /gene="tsaE"
                     /locus_tag="ACHARI_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8443547.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1256..2236)
                     /gene="pta"
                     /locus_tag="ACHARI_002544"
     CDS             complement(1256..2236)
                     /gene="pta"
                     /locus_tag="ACHARI_002544"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8443548.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2389..3069)
                     /locus_tag="ACHARI_002545"
     CDS             complement(2389..3069)
                     /locus_tag="ACHARI_002545"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8443549.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3195..4064
                     /locus_tag="ACHARI_002546"
     CDS             3195..4064
                     /locus_tag="ACHARI_002546"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8443550.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4090..4605
                     /locus_tag="ACHARI_002547"
     CDS             4090..4605
                     /locus_tag="ACHARI_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8443551.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4652..5215)
                     /locus_tag="ACHARI_002548"
     CDS             complement(4652..5215)
                     /locus_tag="ACHARI_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8443552.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5270..5449)
                     /locus_tag="ACHARI_002549"
     CDS             complement(5270..5449)
                     /locus_tag="ACHARI_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443553.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5526..6035)
                     /locus_tag="ACHARI_002550"
     CDS             complement(5526..6035)
                     /locus_tag="ACHARI_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443554.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6131..6511)
                     /locus_tag="ACHARI_002551"
     CDS             complement(6131..6511)
                     /locus_tag="ACHARI_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8443555.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1921 a         1418 c         1190 g         2231 t
ORIGIN      
        1 ttgagctaat accgaaggtc agctgattga acaccgttta ttcaaaaaaa atggactgcg
       61 acacgacttt tgtcacagtc ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt
      121 tctcactctt ttcctatttc ttctattcaa tcaaataact cattttatat acatctaaaa
      181 attcacctag atctgttctt gcccctctag gcatgaccgc ttcgatttgg aagcccattt
      241 ttcggtaaag gtgtacggct ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa
      301 gtacacccat ttccttcgcc caactgatga tttcttctag cataagagtg cctagtccca
      361 ttccccaata ttcctgcaga atactgatac cgacttctcc cacgtgagac aaacgaaatt
      421 gactgtctgc cttgacagat gctgttccaa taatcgtccc ttcatagatt gcaagcagca
      481 ataaattatt attgctttcg tatatataat ccaactcttc ttctaaggtt tctggtggta
      541 acaggagttc tgcttcatcc aaaactaaaa attctgtctc cttattaact tgtcccatca
      601 tcaaaagaat gtcttttgca tctcctggca ctgcttcccg caagatgatc ccaacttctt
      661 cactcatgat tttcagccca tttttctaga attcgctgtt tgaaggtttc actttgctcc
      721 ccgtatgaga ttagtttcac ataccgcttt tctaaatctg tatcactttt ttctaaaatc
      781 agttccaaat aatcctccgg cagtgcttct tctaatagat ttccccattc aacaacggaa
      841 agcccatcac cttcaaaata ttcatctaac cctaaatctg ctccgctagc tgctacccga
      901 taaatatcca tatgatacaa cggaatccga ccttgactat attctctaat aatcgtgtat
      961 gtcggacttt tgatcatttg cgttatccct aatccttggg caattccttt cgtcaacgtt
     1021 gttttacccg cacctagatc accagtcaat atcagattgt cacctggcat tgcggaatga
     1081 ccaattgcct ctgcgaattg ttctgtcatt gttaatcctg atagttcgat catttttcat
     1141 cttctcccgc tcttctttct ttttgccagt atatcgcgta atgaagtttt tttaaagaaa
     1201 aaatgaacta gtcaagaaca gcagctgctg tttttggcta gttcgcgtgt atttgttaat
     1261 caatcaatgt ttgtgctgct gtgataatcg ataatttata tacatcttct tcatttgctc
     1321 cacgagacaa atcagaaacc ggtttgttca agccttgtag aattggtccg attgcttcaa
     1381 agttaccgaa acgctgagca attttatagc cgatatttcc tgactgcaat tctggaaata
     1441 caaagactgt tgcttgacca gctacttctg aatctggcgc tttcagttgt gcaactgaag
     1501 gaacataaga agcatcaaat tgcaattcgc cgtcgatcat gtattgtggc gctagttttt
     1561 tagcgatttt agttgcttca actactttgt ctacttctgg cgatttcgct gagccttttg
     1621 tcgagaagct catcattgct acttttggtt cgatatcgaa caattctgct gtttttgcag
     1681 aatctacggc gatttcagcc aattcttgcg cagttggatt cacgttgatc gcacaatccg
     1741 aaaagacata tttttcttga tctcttccac gtaccatgat catcgctccg cttgtccgac
     1801 tgatgccagg ctttgttttg atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat
     1861 ggatcgctcc agagaccatt ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt
     1921 aattcacatc ttttaaaatc ttttctgctt gttctttgga tgcctttcca tttctacgtt
     1981 caacaaaagt atccaccatt tcatcccact tatcgtaatt atcagggctg atgatcgtaa
     2041 agctagatgt tttgattcct ctggcatgcg ctgcatttct tatttcttct tcgttcccta
     2101 ctaaaatggg ttccacaagt tcttctgctt tcaaccgggc agctgcgcct aaaatacgag
     2161 catcagtagc ttccgggaaa acgattttga tcccacggcg aacaatttta aatcttaaac
     2221 tatcaaacaa ttccacaaga tgacctccag acgtttttat atgtattatc atacactatt
     2281 ccagaaaaaa agaacagttt attttatctg tatcagtcaa aaatgctgga aaaattaagt
     2341 ttttcacata caaagcgaac tgcactataa caggaaagcg ctgttttatt acactgtttc
     2401 aggcagttgc caatcaatcg gttcttctcc taacgcaagg agcgcatcat tcgttttcga
     2461 gaagggtttg gaaccaaaaa agccacgact tgcagataac ggactgggat gtggagatgt
     2521 tatgatgata tggcgactct tatcgatcat tttgattttt tcttgtgccg gttttcccca
     2581 caagataaac acgattggtt tttcccgttc atttaatttt tcaatgatcg tgtctgttaa
     2641 tctttcccaa cctttcccgc gatgagaata cgcttgccct tcccgaacgg taagaacggt
     2701 gttgagtaaa aggactccct gtttagccca tgacactaga tttccatgtt gaacaggtgc
     2761 gattccaaga tcatcataaa gctctttata gatatttctt aacgatggcg ggatctttac
     2821 gccaggttgc acagaaaaag ataagccatg agcttgattt tctccatgat aaggatcttg
     2881 tcctaaaatc accactttaa cttcttcata aggagtcaat tccaatgctt catagatatg
     2941 atacatatct ggatggatct tttgggacgc atattctttt tttaaaaatt ctcttagatg
     3001 cagataataa tcttttgaaa attctgagga caaaacttct tgccaactgt tatgaatgat
     3061 ttctttcaaa aactccacct cctaataaaa agaataactc acattttctt caatcacaag
     3121 ggacaatctt caccctcagc acttgattaa tatggtaaac taggaacaag aagcagaaag
     3181 gaacgataat cattatgatc aaattaattg catcagatat ggacggcaca ttattagatg
     3241 cccatatgag tgtttctcct gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg
     3301 ttgagttcat ggtcgctact ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg
     3361 taggaatcga ttgtgcgatg atcactttga acggggcaca ggtatttgat aaagaaggaa
     3421 attcattgtt taccgtgtcg attccaaata atcaagcaat ctctgtttta gatattctag
     3481 atgctaacgg tatttactat gaagtagcaa ccaacgatgg tctttattct gaaaatcaag
     3541 cgaaacgaat tgaaagtttt gcttccatgg tggctaccca tttacctcac cttacgtata
     3601 aaatggcgat tgccatggcc tcagctaagc tggagttatt gcacatcaca tacgtggaca
     3661 gtattcgtgc tattttggaa gaaaagaatc tggaagtttt aaaaattatt tgttttcata
     3721 cagaagggcc agctgtatta ggtccagttg gaaagcagat ttctctttta ggcgatctag
     3781 ccgttacttc ctctggtcaa aacaatatcg aagtgaacca taaaaacgct caaaaaggaa
     3841 ttgctgttgc ccatgtcgct cacgagcgtg ggatttcttt agaagaagtc atgacgattg
     3901 gcgataactt caatgatgtc agcatgctgc aagtagcagg agtcagtttc gcaatgggaa
     3961 atgcagaaat cgaagtaaaa gattatgcca aatatacaac ggacacaaac cttgaatctg
     4021 gcgtaggaaa agccatattg cgcgcgattg cagaagactt gtagttttat ctagaaaaga
     4081 gggatataga tgtcagaatt tttcattcag gaaaagcagc ttggacgtgc aactcggaca
     4141 attgtcaaaa acgaaaaagg acattccctg tttttgatgg ttggtcgctg gggaacaaag
     4201 ggcgatgcat tgtctcttta tgccatgaat ggcgatctcg ttgctcacat caagcagata
     4261 agcctgacat tcggtacgcg ttttgagctg tataaagagt tcgaaaaggt gggaaccatg
     4321 cagaaaatat tcaattggcc cggtgacttt tattatattc gtagacttca ctggacagtc
     4381 caaggtgata tctataatca tcaatatcaa atccatcatt tcaatcagca aatcatgaaa
     4441 atggataaag caacactttt ttcaggagat tactatgtat tggatgtctc aaatgatgaa
     4501 gatgctccag tttgtatttg tattgctgcg gttttagatt actggctcta ccgtaaagac
     4561 aaaagtcgcg ataagcctgt ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa
     4621 atgcggaaat acgcatttca ggtacatctt tttagatatt cttttgcata tgatcataaa
     4681 gatgaccact gatcatccgc tgaccgacaa ctttgaagtc atggcgttcg tataattttt
     4741 tagcctttgg atttttttca tccacactta gaccgatgac ggagcgatta gctttcttcg
     4801 ccaatttagg taatgcttct aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg
     4861 atacactgat agagtccaaa taccattcgt tgggaaacgt ttctttatca atgaacaaac
     4921 gtgtagacgc atctagctga tatttctcta agataggtac cagtggctga tcgatgattg
     4981 attcttcttc atctgtataa ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta
     5041 cgatacctcg tgaataactg taacgataat ttggatcagt gattgcttct tctaaaatct
     5101 ctaaagtctt ttgttcccct gtctgcttca aaaatggcag ttccatatct tttagaatca
     5161 ctaagatcaa tggggcgatt tctggagcat cttcttttct cgcaaaacga atcatagttt
     5221 cctctccttt tctccattct aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa
     5281 aatcttcaat cttttcagta atgaaataac catgagtaac gattcttgcc gcttcagttc
     5341 ggcaataagg gcagacgact ggcatttctt tatcattatg ttcgactaca ttattgactt
     5401 cttcgtatgc aaattttttt ccgcaattag ggcatttcat tttttccatg ttatctcaac
     5461 ttcctttcat ctttactctc ttagcttact cttatattgt aaaaaagaga agcaatatcc
     5521 ttcttctatg cagatatcca tacctgacgg tcctgttctc catctattaa tcggacttta
     5581 taataaggac gttcatcttt gactagaagt gtccactctt tcacttttgc ttttttcccg
     5641 tagtagctga ctgcttcctt catcgctgct tgcggttgtt tgaactcttc cgttttttct
     5701 atatcaaaaa gatgttctgc ggccttttcc gtgtcatttc gtacggttgt tttcccagtg
     5761 gatggattga ctgctatgac ttgtccttca caaaaaaact ggtattcata tgttgcattc
     5821 gtaggattct ccaaatcttt ttcaactgca aaatgaatag cagatatgtt atgttctttt
     5881 gtttcgtttt ttacgattgc tgctgctttt ttcaatgaga tccgatattc ctggtcacta
     5941 atatcatcca tgtgaaaagc ggcattgagc agtcccgtca taccggtaat ccctattacg
     6001 atgagggcag caacaccaaa catcattttt ttcattcttc attctcctgt ttcttttttt
     6061 taaagaatat caagaaatga acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat
     6121 tttgttatct ttattcgtgc agttctaatg gcaatccgtc tggatcaaag aaaaacgtca
     6181 ttttttttcc tgtaaaggta tcttctctaa tcggttctga ttcgatccct aaggactgca
     6241 attctgcaat tgtttcttca atatcgtcta ctttgaaagc aagatgacgc aagccacaag
     6301 cttctggata gtttggtcgt tgtggcgcat tagcaattcc aaaaatctcc aattcagcac
     6361 ttcctagttt cagatctaat ttatagtcat ctcgttcttt gcggtaattt tctctgatta
     6421 cttcaaatcc taatttatta acataaaaat cacgtgattt ctgataatct gaaacaatga
     6481 tggctatatg atggatcgtt gtaaggttca tatgagcact cctattcttc gtttaagctc
     6541 ggatctatat taatctccag ttccattgta aatgtgtcag attgaaaaaa atagctgttt
     6601 ttccttttta taagaaaaaa ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc
     6661 ctgttcccaa tataataaat gaaagtaaga aattatattt tcaagaaaac aaacagaaaa
     6721 ttatcataac acaaattatt tataattgaa agactagttt
//
LOCUS       DAYYAO010000116         6757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000116 DAYYAO010000000
VERSION     DAYYAO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.121"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..412
                     /locus_tag="ACHARI_002552"
     CDS             <1..412
                     /locus_tag="ACHARI_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8443556.1"
                     /translation="ESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDY
                     RQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKT
                     DHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            488..706
                     /locus_tag="ACHARI_002553"
     CDS             488..706
                     /locus_tag="ACHARI_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443557.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            693..3371
                     /locus_tag="ACHARI_002554"
     CDS             693..3371
                     /locus_tag="ACHARI_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8443558.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3362..3559
                     /locus_tag="ACHARI_002555"
     CDS             3362..3559
                     /locus_tag="ACHARI_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443559.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3563..4174
                     /locus_tag="ACHARI_002556"
     CDS             3563..4174
                     /locus_tag="ACHARI_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8443560.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4159..5550
                     /locus_tag="ACHARI_002557"
     CDS             4159..5550
                     /locus_tag="ACHARI_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8443561.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5766..6080
                     /locus_tag="ACHARI_002558"
     CDS             5766..6080
                     /locus_tag="ACHARI_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443562.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6073..6567
                     /locus_tag="ACHARI_002559"
     CDS             6073..6567
                     /locus_tag="ACHARI_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443563.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
BASE COUNT         2578 a          926 c         1318 g         1935 t
ORIGIN      
        1 tgaatcactt tcaactaaaa ataataaatt agatgaagaa ttgatcgaaa catttattga
       61 agaaaatctt cttaaacaaa tatggggaga aagtattgtg aattgcttaa aactggctag
      121 caatagtgat tatcgtcaat tcgacaactg gtacaaaaaa ttcaaatatg cgattagatc
      181 tgctgaaaaa gaacaaaaaa ttcaactgaa aattatctat gaaatttgta ataacaagta
      241 ttttgtagat catgttagag aacagctatc aatgacttta cgtgacttga tacgaagagc
      301 aaaaacagat catcgtatca aacaaaaaga cgattatatc tttgcttctc taaaaaataa
      361 agcgttagaa ctaattgaat tgcaaatcag cgaaggaata gataagcaat aagaatagaa
      421 ttgagtcaaa tgaaatggag gggtatctcc acttcatttg agaactttga atataggagg
      481 ggttattttg attaaaaatc gtttaataga tcaagtttgg tgtctagatc aaaatacaga
      541 tagtctcgaa ttaatcgaga ctatttgctt caacactata gttctcgatt taagagctga
      601 aaatgataat tgtgtaactc atgtcataat taatcaaaaa atggctcaga agctctctga
      661 gagcctcaga aaatgggcta agggaggtaa cgatgaaaat aattgaaaaa aagattgcca
      721 ataaaaaaat aaattggttg gttgttaatt ttcttgttct gatagctatg attattttta
      781 caagtaatat tgctcatgcg agtattttca attggtttga tgacacaaaa agtcaaacag
      841 agtttttgaa taattctctt tatagacctt acctaaaaaa gtacaatgga gatttcctta
      901 gtcagttcgg gatttggctc ggatgggctg tggtaaaagg catgtttact gtgacagatt
      961 cgattcaaaa tatgattcca gatgtgctgg atttgttcaa tttcattgaa agtacagggc
     1021 taaataatgt ttatcaatca gccgtaaata caattgtcat tggattaatg atcctttcat
     1081 tgatgtttgt tggttacaaa atgattactg gaaaaggaac agtggattta aaatcggttg
     1141 gaatgaacgt agtgatgtct atagcattaa tacttttaat gccaaccatg atttctagtg
     1201 gtattcaatt tgctaaaaca ttttataagg actctactac gattacaaat tcagatgacg
     1261 gagtcgcttg gtcattgata aaacaaggag taacagattt agcatatatt aataaaacag
     1321 atcagtactc aagcattgat aaaacggagg atcgaaataa tttaacacga aaaaattttc
     1381 aacagacgga tctcacacaa gtgttaacgg ataaagtgat tgataaattg gaaaaagaaa
     1441 atccaattgc ggacaatctt cgttatgaat tagttgaaaa ttctaataat gaatttgttg
     1501 ctacaaagtt ttctgacaac ttcttatcaa cattttctga tagcttgaaa tcaggctatt
     1561 accgatacca agcaaatcta tgggggattt caattgggtt gacagcgtta gcaatcgcct
     1621 atgtgttcag cgcgtttgtg atcattacag caatactaga acttgcattt aaacgtgtgt
     1681 taggtgttct tgtttttgca acagatattg agacaggaca gcgttcaaaa gttgttctat
     1741 cagatattct acaatgttac ttaactgttg ggtttcaagg atttgggtta tcaatgtttg
     1801 ctatgtttat caattttctg aactcaggac aaggtatctc aacaaatatt tttataaaaa
     1861 caattgctta tatctgtgcc gtttttgttt taatcaaagg cagtggaaca gtcatgagat
     1921 actttggtgt tgatattgga ctcaaagaag gctatgggca acttgcttct gcttttggaa
     1981 tgggcgcaat gctgtttaga aaaggatcaa atggatttaa tcgtgctaaa ggaagcggag
     2041 aaggagaaga tcgaaaacca gaaaagaatt tcggcgaaac tctatcaaaa aaagctggca
     2101 aaactggaag agctttggga tatgcccatg aacgtggatt atccggttta gcttcagatg
     2161 gagctacaat ggctagtgaa agagcaacaa aaccatttaa gagtatgcgt gatatagcta
     2221 atgataccaa gaataagttt aaagaaggtt tagatgatgg tacagtgtcc gctattaata
     2281 aaaatagcaa acctatgtta gctaaaaata aagaagatga aactggaaaa tatgctgatt
     2341 ccgtgccaac acgtctttca gatagaaaag atggtgctaa agttgaaaat gccgatagaa
     2401 ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa tcgaataata
     2461 atcctgcaag cgcaattcag caaaaagtcc aacaagatat tgaagaacgt aaaaatgcta
     2521 tgcacggtca agctaagtct gctgaagaat taattaatca gaagcgtcat gaagcgaaat
     2581 acacgcctga agcaatgaat cgcgaggaaa taatgttaag gaaacgtgta gaagggcgga
     2641 caggcgctaa tattgacgga aaagaagcgt tgaccaaaga aagaattcaa gaagctaaaa
     2701 attcaaatac aggattagaa aaattagtca aagaaaatcc acaaaattct acaagtagtc
     2761 atggtggccg atcagtagat gtccgagaaa atattcaagg ttcactgaat ggtcatgttg
     2821 gacggaatgt tgatgtaaga gagaatttac aaagttcttc aaatgctcaa ggtactaaga
     2881 caattgatgt tcgagaaaat ctgcaaagta gcagtacaag tcaaccaaaa acagtagatg
     2941 ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt taaatcagtg gatgtccgag
     3001 agaatgtgca aggtagcagt gcaagtcaac caaaaacagt agatgtccga gaaaatgtac
     3061 aaagttctac aagtagtcaa ggattcaaaa cagtagatgt tcgggagaac attcaaaaag
     3121 atagtggaga aatgaaagaa aaaacacaaa aaatcaatat cgttgaagac agaaagtcag
     3181 cgaaaaaatt tgattcaaat catgaaacag tgattattga tagtgaaatc cgtgaaaatg
     3241 ataagggatc aaaatcaaga cgaagattca cttatgaaga caatgagtta ttcagagata
     3301 cacttaatga tcctaatcca cttttcgata atttattaag aaataaaagg gggggattgt
     3361 catgcaatta attctaaaga ttagtacgca tgatttctta gaacttgacg aattatgtga
     3421 atcagtaaag aacgcaacaa atgaaaaaat cacggtcact aattatattg aactgatgat
     3481 tcaagaagaa ttaaagatga aaaatgaaat agagagcaat ttcaaatgtt gctctctaga
     3541 aaaaaatgtg aggggataat caatggatga taaacaaaaa atgtacgaaa aaaatcgcaa
     3601 acatagtgag gccttaaatt ttcgtgtcga taaaaagact tatcttgaac tgacattgat
     3661 tgcaaaacaa aggggaattt ctagagcaga cgtttgccgt gagcggttaa agggactcaa
     3721 atatgaaccg ccgttgttag ctgaagatga tagggtagtt ttaaccaaac agttaaatca
     3781 aattggtaac aatttgaatc aagcggtaaa ggcattaaat atcattgcta aatattttag
     3841 agaagaatct gaagataaaa aagaaaagag cctacatttt agttttgatg ctagccgtta
     3901 caacattttt aaagatgaac ttgcgaatag catagcaaaa aagttgatga ttagttatcc
     3961 agatgacatt tttttggagt ccaatcggag tgccccctct ttttttaaat tatcagaagt
     4021 agaaaaagaa tatttcgaat attggataga aaagcaaact agaccgggta cgaaagaaga
     4081 gtattcccaa aaagttgatc ggaatttaat tcactcaatt cagattattg caagtttaaa
     4141 agatgaggtg aaacaacttt gggagctact ataacatcga aaggacgcaa gaaaaattta
     4201 tcctcgctat tacaatatgc agctaatcct tcaaaaaaag aaaaatgtgt tgcagtctca
     4261 ggaataaatt gtagtgatga tagtgaggaa gcactgcaag aaatgaattt agtacgtacc
     4321 aagatgaata aatctaaaga tatagcacaa gcgtttcatg ttattcattc gtttgataag
     4381 agtacttcta aagagttaag tattgataaa atgcacgaaa tagctgtcga atttgcagag
     4441 aaagcttttc caaattcgca aatcattgtt gcaagtcaca atgataaaga tcattttcat
     4501 agtcatcttg tcattaataa tattaacatg gaaacatgta aaaggttgcg tattgaccct
     4561 aaagatttag aactaatgcg aaaaattaac gatcaagtcc tcaatgaaca tggtcttgaa
     4621 tcagtgaata atgagtacta tgaaaactta gaaaagagaa ccaatcttta ttcaaacgat
     4681 ccttctaaga tgaaatatgg aaaaattaat tatcaagagt ttgtgcaaaa tgcgatcgaa
     4741 gcaacgttgg aaaaatgcga agtgaattct tttgaagatt tttcggatca tttggcaaaa
     4801 gaatataaca ttgaagttta tcaattttca aaaacaaata acaaacttgg ctatgttctt
     4861 tacaaacaag gtgtaaattt ctatgagcgt agaggtgagt taattacttt aggaagagat
     4921 aaacaatcta gaaaagaaaa aagtcagtat gtcgaacgta cttttagtgc aagaaagtta
     4981 gggaaaagat attcatttga atctgtttct gaacaacttg aaaagaattt agaagaatca
     5041 catataaaaa atttttttaa tatggaaaga gagcaagtta tgtcgctaat tagtggagta
     5101 aattacaaaa ctggtgagtt aaatgtaccg tttgtggaat tagattccat cgcacaaaag
     5161 caatttagcg accgtgaagg cgatgaaaaa ttcattaaat atgatgactt cttgcgagag
     5221 gtagacaagc attttgtaaa tacaatgtct ttagaagatg atcaagaata cacgattaaa
     5281 cttcatacgg atgtgaatcc tcaaacttta cgtagtgcga aaagtaaaac aatttctatt
     5341 aaaaagactg atcagaaaga gttatttcta ccggcaaagg gttttgaaaa ttgttctcat
     5401 acaccttctg ctaaacagat tcactatcaa atcagtcgca taatgaatga cgatgtaaaa
     5461 atgtatcgtg ctaaaaagga ttatgaattc acacaacaaa tgattgaaaa tcagcaaaaa
     5521 aggcaacaaa attttgaaat ggaattctga atgggcttgt tagtggactt tccagttttt
     5581 tgcctatgaa gagttgcttg gaaagaagca gtttttagga catacgccag ctacccatct
     5641 acttgccagt caatccgccc cgctcacgct aggctacttt ctggcaagtc agatggcggg
     5701 taaatctctg agggagcaag ctccctacag actccctccc aaaaaaagaa aggtggaaga
     5761 aaaaaatgaa ctatccatat tttaaagtct cggcttcaga agaaactaaa gaaatattca
     5821 acaattttta caaccaaaac aaaggaattt ttggttcaaa ggcaaatatg tttcgtgtta
     5881 tggtttcaaa tttaccagtg ttagctagtc catcaaataa taaatttaat gatcctgaaa
     5941 gcattaaatt tgaacaaaaa atttcagaac tagaatcaat gataagtaat gaagtaattg
     6001 aaaagctgga tgacattgat caaaagttat cgtattctct aaaaaacaaa tacaaaacgg
     6061 aggagaaaaa agatgtttaa agtttacttc ccgtttcagt ggctcgttaa aggaattctt
     6121 ttagtgctac taggattgat tctttttgga agttatctaa caattaaagc cagacatttt
     6181 ccagaagaaa agccaataaa aattgtaaca aaagatttaa aaataggtga aacttatcag
     6241 gcagatgggg ttgaactaac atttaaaaat attgaagtga ccgaaaaaag aaatgaattt
     6301 gcacaacatg atcgtgtatt acaagtgacc tatacactta taaatgatac agaaaatccg
     6361 atacgtccac aacaatggtc gattgaaaat aaggatggtg aagaacttta tgagtatgat
     6421 atgatattgg ataatccaga gtatattgaa agtggcaaag aagaaacagt aaattactat
     6481 tatgaattac cgattgaaag gcaggactac caaatcgtag tacgtagaga gaatggttac
     6541 atagttgggt tgccatttac tttataagtt ttattttgtt agtagttttt aaggtaatat
     6601 cagttttgtg atttttgaaa atggaactgg gagaatggct gaggaaaata aagttgttcc
     6661 gtttgaaaca ttagtgataa ataaggaaga gtttgagcaa ctttttcttg atttttttat
     6721 tcaatatgac atgatttatg attttagttc gtcacta
//
LOCUS       DAYYAO010000117         6692 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000117 DAYYAO010000000
VERSION     DAYYAO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.122"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..314
                     /locus_tag="ACHARI_002560"
     CDS             <1..314
                     /locus_tag="ACHARI_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443564.1"
                     /translation="LQYLLILGLFIIINFQMIFVSFTINSKKIALKKIFGYSPISQVL
                     DLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIIIYKKNSFKYFKENI
                     L"
     gene            311..931
                     /locus_tag="ACHARI_002561"
     CDS             311..931
                     /locus_tag="ACHARI_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8443565.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            1045..1182
                     /locus_tag="ACHARI_002562"
     CDS             1045..1182
                     /locus_tag="ACHARI_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443566.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            1624..2004
                     /gene="mobC"
                     /locus_tag="ACHARI_002563"
     CDS             1624..2004
                     /gene="mobC"
                     /locus_tag="ACHARI_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8443567.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            1986..2900
                     /locus_tag="ACHARI_002564"
     CDS             1986..2900
                     /locus_tag="ACHARI_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8443568.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            2860..3138
                     /locus_tag="ACHARI_002565"
     CDS             2860..3138
                     /locus_tag="ACHARI_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443569.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            3140..3829
                     /locus_tag="ACHARI_002566"
     CDS             3140..3829
                     /locus_tag="ACHARI_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443570.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3950..4036)
                     /locus_tag="ACHARI_002567"
     CDS             complement(3950..4036)
                     /locus_tag="ACHARI_002567"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8443571.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            4681..5418
                     /locus_tag="ACHARI_002568"
     CDS             4681..5418
                     /locus_tag="ACHARI_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8443572.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            5446..5985
                     /locus_tag="ACHARI_002569"
     CDS             5446..5985
                     /locus_tag="ACHARI_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8443573.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            6001..6171
                     /locus_tag="ACHARI_002570"
     CDS             6001..6171
                     /locus_tag="ACHARI_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443574.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(6483..>6692)
                     /locus_tag="ACHARI_002571"
     CDS             complement(6483..>6692)
                     /locus_tag="ACHARI_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8443575.1"
                     /translation="IFVTILGLFELIQMNFFSFTHGIFTWFALSGLIVIDILNYFYIY
                     YSYLKNEKFFYTMFFPLVILIILLS"
BASE COUNT         2591 a         1047 c         1140 g         1914 t
ORIGIN      
        1 atctacaata tcttttaata ttaggattat ttataataat taattttcaa atgatttttg
       61 ttagttttac tataaattca aaaaaaatag ctttaaaaaa aatatttgga tattcaccaa
      121 taagtcaagt tttagattta tgtttactcc cttttacaat agagttattt gctactatgt
      181 gttttcaatt gatattttca aaaaacactt acttgtattt tataattttt atgatctttt
      241 taataaatct aattacttct attattattt ataaaaaaaa tagttttaaa tattttaagg
      301 agaatatttt atgatattat cagttgagaa tttcaccctg tcaattaata aaaaaaagat
      361 caaacaaaat tggaactttt caatatcaga aaaagatttt attatagtaa caggaaaaag
      421 tggtagtgga aaaactactc tgttaaataa tttaagccta atagataaac aatataccgg
      481 taacttattc tattttgaaa agaaagtttc taaacatgat atacaacgtc tacgaagaaa
      541 tcatattagc tatttatttc aaaactatgc tcttcttgaa aatcaaacag tacaatacaa
      601 tttcgaatta gcaaaaaagt ttaacagtaa tatttccaat gatcatattt ataatttatt
      661 aacagatttt tctttgccaa aagatatctt aaaagaaaaa atctttctct taagcggtgg
      721 agaacaacaa agaatagcat taattagaaa tatgttaaaa ccatttgata tattatttgc
      781 agatgaacct acaggaaatt tagataaaga aaactcaact tttataatga actttctgaa
      841 aaatttaaca gaaaaagaaa aaaaagccgt tgtactagta acacatgact taaatgttct
      901 aaactatgga actaatatta tagaattata atttttaccc gtgatgctag ctgatttaaa
      961 actagcacca cgggtattta ttttatattc catctctcct atcccaaaat ttaaataatc
     1021 acttaataaa aaggaggcac cctagtgatt ttattgaaaa aagagtatga aaaattttat
     1081 gatagactta tttattcttt gcaattgcca aaaaaattaa accgtcaatt acaaaagact
     1141 tatgaaacta aatcaactga agaatgggag agttattgct agatttttgg tagaagtaac
     1201 aaaaagtgga catattatca taatgactcg ccgaaagtct gataaaaaat tggctaaaaa
     1261 tattgctgaa tgtttaataa aactatcagt tttagctaaa tagttagtag tttacacaaa
     1321 aaagcattta tgttttttgt tgcaaagtaa caatttcatc ttcggtgaaa taagcgaaac
     1381 tttacaaaaa gtggctagca tttgctagct ttttttgtat tgtggagcgt ggcttttgag
     1441 ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcact aaatacgtag
     1501 gtatttggct agcaaatata gagcttgcca aaccctttta gtgagcttta ctcattgtac
     1561 ctttttaggt acaatggtat tgcgaacgat acgtccgtag gacgtactga aagggtggga
     1621 cttatgagcg aacaaaatca aaatttggct agcaattctt ctaaaaaata tacctatcga
     1681 tccgagccaa aacaaatcag ttttcgagtg agcgaatccg aatttgcaaa gctgaagcaa
     1741 tcggctgaag ctttgcaaat gagtgtgccg gctttcgtga aagccaaggc acaaaacgcg
     1801 cgtctggtga caccgaaagt ggcgccagac attgcccagg caatcgcgcg cgatttagcc
     1861 aaagctggag ggaatatcaa tcaaattgct aagtggtgca atacacacca acatgacgtt
     1921 gcttctggtg acgctcaacg tttatccgaa aacctaaaaa tcatgcaaaa ggagctacaa
     1981 aagatatggc aacaattaag gtaagccgaa ccacttcttg tagtcgtgca attaattacg
     2041 cagaaccacg agcaaccatt aaaacaggaa taaactgtga cgtcgattat gccaaaagtg
     2101 aaatgaaaca aattcgcatg ctgtatggaa aagacgaccg tgtacaggca cacttattga
     2161 ttcaatcttt tcgaccagga gaaattaccg ctgaaaaagc taatcaacta ggggcagagt
     2221 atgcaaaaaa aatagccccc gatcaccaaa cagctattta cacacataca gacaaagacc
     2281 atattcataa tcacattgtc attaactccg ttaatctgga gactggaaaa aaatttcaag
     2341 ctcatggtca agcatttcta aacaaatgtt atgacctaaa tgacgagatt tgtcttgcac
     2401 ataacttacc aatcactgag cgaggaaaga aaccagaaaa acgaaccatg tctgaaataa
     2461 aattaaaaga aaaaaatgaa cctgtttgga aagacgaaat tcggattgcg atcgatcaaa
     2521 caatgaaaaa caaaaaaacc agaacctatg accaattttg taattccctc aaaacattgg
     2581 ggattcattg cttcaatcgc gggaaaaatt tcacttacga attgatccac aaaaagaaaa
     2641 aagtacgatc caacagacta ggaaaggact acgaaaagga gacgatttta catgagctgg
     2701 acagacgaga acgaacctat aaccaacagc gaaccaacga gctacgaaga gctcgagcta
     2761 gcattcgcga aactgcagaa gaagtacgaa gagaacaacg aactcttacg cctgaacaac
     2821 cggaaattag accggctcga agaaaccatg aacaacacgt tgaaagagga acagaccacc
     2881 aacaagaatt atcgctctaa aacgttaaaa gacatagaaa acgaaataaa gaaacaacgt
     2941 gcttttctcg ttccttatac gcaagaaagg atacaaacta cgattgattt acaagttaaa
     3001 aaactccatc aagagctatt agaccagcga aataaggaac agaaaaaggt gcttattcaa
     3061 cgatcattgg attttcttgc ccccttatgt tgcttagtcc tgacactgtt cgcattttgg
     3121 ttagtatggg gaggctaaaa tggcatttta tttaccctat ctactcattt ttgttagtat
     3181 ttcaggaagt atttggctca tctataaaat ttttcaaaca agatattcat taaaaggctc
     3241 taaaattcga tttaagcgat ttttctttct tggctgtata ttttccttga taatcgtttc
     3301 aagcggctta ttcggcgttt tagagggaaa taagcgcgtt tctagaagta ttttattagg
     3361 aaatgtcact caaaagtatg aatcagcgcg tgataaaaag aaaaaagagc aagcattagc
     3421 acaaaaaatg gaagagttta ccacttgtta tgaggaaatg aacgatattt ttgtgaatca
     3481 agaaaaacgt ttgacggata aaaacatgga aaaactcact cgtctttatc aaaacttgcc
     3541 agaaaaacag caaaaagagg ttcaagacaa ctatgagcga accaaaaaag acgtgcaata
     3601 tgtaaaagat acaaagatag aagaagcctg tagtgatcta tttggagaca caaacccttg
     3661 gtttgccagt gaagaagaaa aaaaagaaaa acaacaatct gtcacatatg aacgctatga
     3721 aatcttactt caacaagcaa ctaacatgca aaatccgaca aaaaaagaaa ccgccttaaa
     3781 ctatctaaaa agtgtcaagg aatggttaga tcagcaacaa caaaattgaa tttgatttaa
     3841 aatcatgtta aactgaatgt gcaaatgatc aaacattaga tacacgaaag ccccagagag
     3901 gtcggattct ctggggcttt ttttcaggtt gatgtcgcct ttattgattt tacttcttgt
     3961 ttcggttatt caaccaataa ctgaataaag agacacaaac tcctacaaga agggggcaaa
     4021 tgaacagttc aaacattaga tacacctcct tccagtcgca agactgtcgg ataggcgaca
     4081 agttattata tcttaaaaaa ttaaatttgt gtggtgtttt taagtatcac ttgttttagg
     4141 gttgttaggg cttgccctga ccgcctgtaa ggcgcttctc gactagatta agggaaatta
     4201 atcaagaatt gtcttagaac ggcttagaca agctttcaga gtctcacacc tcctctttga
     4261 acaacggttt tagcaaagga gccggaggcg actgcgcagt ataatatgag agcatagcga
     4321 tcacaccttt ttagcgaccg tagggagcaa gttttccagt ttagaaaaat tcgcgaacct
     4381 ttttggagga aaaagtagga gttttttagg cgggaaattc aatgtgctct taagatatac
     4441 tgttagatat actgttagat atactgttaa taataactgt tagggattag caaccttatg
     4501 ggagagtaag tattgacata ggtagtgggg gataaattgt cacactatgg gggataaatt
     4561 gtcacactat gggggataaa ttgtcacact atgggggata aattgtcaca ctatggggga
     4621 taaattgtgt tttacatcct cttatgtcta tgataacata atttattgag gtgattttta
     4681 ttgagtaacg atatagttcg atataataat ggattaaatt ctgtcccttt aagaaaattt
     4741 acccctatag atatggattt attttgggga atatgttcta aaatgaaaag aaaaggtaca
     4801 aaagaagaaa tttttacatt taaacaaata aaagaattaa ttgattacaa tataaatgca
     4861 cgtggcgacc gttttgctaa tgatttaata aaaatgtcaa aaaaaatcat tactttgtct
     4921 tttatttatg aagacgaaaa aacatatgaa caattaaatc tgtttcaaaa atttaaaatc
     4981 gataaagtga atgaaacatt attaataaag gtttctgaag aatttgaata tatacttaat
     5041 tcaatcggaa ctaattttac tcgtttcgaa ttagaaaata tgacaaactt aaagagtagc
     5101 tacgttaaag agttatatcg ccaacttatg gctcataaag acagaagatc taaaaaaggt
     5161 cactggtttg taaaaataga agattttaga gctctattag caataccaga aagttatcgc
     5221 atgagagata ttgataaaca aatttttgcc caagcgaaaa aagaattttt aaacgttaat
     5281 tcaactcacc ctgctatttt taaaactttt gaaatcaaaa aaattaaagc ctacaaagga
     5341 aataagatag gatctctgca aatttatttt gaagaaaata taccttcaat taccttagat
     5401 aattggttag agaaataata cctaacaatt taggaggcaa ttatcatgca acccaaagaa
     5461 acctttacaa taaaggaatt atctgaatta tttgaaataa gtaggcaagc aatctctaaa
     5521 catgttgata aattagaccc gtccttatta gctaaaaatg agcgtggtta taaggttgta
     5581 ttacgttctg gagtattgca acttgctagc aacttaggta accaaaggtt actatatagg
     5641 ttgcatgagt atgaacaaga aaacgataaa aacgtttata tatcaagcaa aaacgtgatt
     5701 gtaaacgagt tgctagataa gttgcgtgaa gaaaatgaat ttttacgaga acaactttcc
     5761 ctcaaagagc aaaataattc taatatgcaa aaattactcg accagcaaca acagctcacc
     5821 ctacaggcaa atcaacagat cgaaaaactg caagaacagt tacaattagc ttatacggaa
     5881 gaaactccag aagatgaatc aactactatt ctttctgaag aaacagaatc gatagaaaaa
     5941 cacacaaaaa agaagaagca aaaatggtgg caactttgga aataactgga gggaacattc
     6001 atgacaagaa aaaatcttct attcggttct attttactag gaattctagg cttcagtccg
     6061 atgttattgt cagtaatcaa taaagggaat aaacatggtg aattgaaatt gaatccaaat
     6121 tattacaaca gtcctaacga agaattgttt gtaaaagaga cggataaata ataaaaaaga
     6181 ctgctcttcc gagcagccag ttaaaaaata atgagtgttt attcttgaaa aagcacgcgc
     6241 ttatgaagcg ctaccaaaaa tttaccatat tttacaaaaa ataacaagtc ttaaaaaaat
     6301 gacagataaa aatgcacaaa gaattagata tagataaccg cgaaaaatca agctttagaa
     6361 agttgctttt agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga
     6421 ggggctactt ggcatagtcg acattatgtc aactatgcaa ttaaataact aactacttca
     6481 tattagctta ataaaataat caaaataact agggggaaaa acattgtata aaaaaatttt
     6541 tcatttttta aataagaata gtaaatataa aaataattta agatatctat tactattaat
     6601 ccagatagag caaaccaagt aaatattcca tgtgtaaagc taaaaaaatt catttgaatc
     6661 aattcaaata aacctaatat agtaacaaat at
//
LOCUS       DAYYAO010000118         6642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000118 DAYYAO010000000
VERSION     DAYYAO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.123"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHARI_002572"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHARI_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHARI_002573"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHARI_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8443576.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHARI_002574"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHARI_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHARI_002575"
     CDS             complement(2143..2373)
                     /locus_tag="ACHARI_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8443577.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHARI_002576"
     CDS             complement(2603..3421)
                     /locus_tag="ACHARI_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8443578.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHARI_002577"
     CDS             3582..4271
                     /locus_tag="ACHARI_002577"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8443579.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHARI_002578"
     CDS             4285..5787
                     /locus_tag="ACHARI_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8443580.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHARI_002579"
     CDS             5800..6276
                     /locus_tag="ACHARI_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8443581.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHARI_002580"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHARI_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag at
//
LOCUS       DAYYAO010000119         6601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000119 DAYYAO010000000
VERSION     DAYYAO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.124"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(252..1463)
                     /locus_tag="ACHARI_002581"
     CDS             complement(252..1463)
                     /locus_tag="ACHARI_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443582.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACHARI_002582"
     CDS             complement(1581..2216)
                     /locus_tag="ACHARI_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8443583.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2299..2721)
                     /locus_tag="ACHARI_002583"
     CDS             complement(2299..2721)
                     /locus_tag="ACHARI_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8443584.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2739..3059)
                     /locus_tag="ACHARI_002584"
     CDS             complement(2739..3059)
                     /locus_tag="ACHARI_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8443585.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3352..3543
                     /locus_tag="ACHARI_002585"
     CDS             3352..3543
                     /locus_tag="ACHARI_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443586.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            3557..3808
                     /locus_tag="ACHARI_002586"
     CDS             3557..3808
                     /locus_tag="ACHARI_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM8443587.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            3784..3963
                     /locus_tag="ACHARI_002587"
     CDS             3784..3963
                     /locus_tag="ACHARI_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443588.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(4006..4476)
                     /locus_tag="ACHARI_002588"
     CDS             complement(4006..4476)
                     /locus_tag="ACHARI_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443589.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            4563..5261
                     /locus_tag="ACHARI_002589"
     CDS             4563..5261
                     /locus_tag="ACHARI_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8443590.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            5439..5780
                     /locus_tag="ACHARI_002590"
     CDS             5439..5780
                     /locus_tag="ACHARI_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443591.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5773..6444
                     /locus_tag="ACHARI_002591"
     CDS             5773..6444
                     /locus_tag="ACHARI_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8443592.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
BASE COUNT         2146 a         1081 c         1176 g         2198 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 tcgttattta atggtatttt aaatgaagat aagcgaattt acagcatttt taaatgctta
      181 tataccgcaa attatcccaa gtttttatat atcctacccg agtttatacc cgaaaatata
      241 attttttagt gttattttat atcttttttt aatagttttt caaacgctgt agttgctgta
      301 tctttttctt tgcgtaagac atggccataa acattcattg tagtattaaa atctcggtgt
      361 cctaatcgtt tttgaagctc ttttggagtc atattaggat cgctaagcaa ataggtagct
      421 gatgtatgtc taatgtcgtg aaaacgaatg tgacgcaaat tgtgacgttt ggtgaatcgt
      481 ttgaacttct tatacagata acttccacgt ggtaaagttc cgtcttgttt tgtatcaaat
      541 ataaaatagt gtcttaattt ctttatattt aatttccaac gaattttttt attctctttg
      601 atcaattcat ctaacatatc aaatagataa gcaggaccgg ttactatttt ttcatcatca
      661 tcatttttca aacctggcag caaccgaata tctgattttc catccacttc tgaaatccgc
      721 tgatgaaatc ttatttcttg tttgtcaaag tcaatatctt tttcttctaa agctgcaatt
      781 tctccttgtc ttgctcctgt aataaatgct agaacaatta aagttttaat ttctatgctt
      841 tcctcaaatg ctgcttttag cattgcatga atttcttctt ccgaataagg ttcttcgata
      901 ttctttttta cgcctttttc ttttggtatt tttacatttt ccacaggatt ttctttaatt
      961 gctccgtact catttttagc catcaaaaat aagttgttaa cggcgtataa catacgaagc
     1021 tttgtatttc ttgataaagg tttatcagag tttcttccag gatcttttgt attgattctt
     1081 tgagcattaa caacaatttt ctttatcatg tatggtttta tatcgattag agggataaaa
     1141 tcaaatttat caagaaatct attctcgatc attcgcttat agttatgata agactttggc
     1201 tctaaatttt tttcggcctc tgaaagccat atttttctat aaaattcacc aaaagttata
     1261 ttgaataaat ccaacgaatc atcggtcatt ccttcgaatt gctctaacca gatgttcaat
     1321 tctttatatg ccgctctttt actattcgct tttatagttt tgctttttct ttttgccgag
     1381 ccatcaggtt tgtaccctaa tacagctctt agtctatatg tttcattgtc tacttgttct
     1441 aagtatcctg ttacttctga cataattgaa tcagctctct ttctctgata caataggtac
     1501 tataaagaag cctattgtgt aggtttgttt tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttatga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tgtgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atatccctca cttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggcttttttt actgtgcata agagtatttt ttcttgaaat atgactggca
     2341 aacattaaaa cattctgttc ttaacttatt attgatagag tagaattcca tgaaattttc
     2401 taatttgaac tgagattcat cagtcaattc gttctcaata aagatattta gtaaaatcat
     2461 aattgcgatt ctatcagctt cagcttcgaa ttttgaatga aaagttgtag agttatcgta
     2521 cagtactgaa tattcaaaat gtgaagcaat gaaatgaccg agctcatggg ctaaatgaaa
     2581 agcttcagaa ctgtcttcgt gtagtttttc attcaaaaat actattcttg gttttggata
     2641 ataaaaacct ggttcttcca tttccatata gattaacttt aaattatact cactcagcat
     2701 ttctttcaac tttaaataca tacaacccat cactccaact attcattttc ctctaaagct
     2761 ttagcaattg caatcgcttt acgcattgtc tccttagata tttcttttcc atcaaaagaa
     2821 aatacagtat catcttctga taaatccaca tgtttaggcg tttctttttc ttctctacct
     2881 agaagatagt ctacagagac attgaaatag tcagctacct tttgcaaatc tcttgctttg
     2941 ggttctgttg ttttccatcg atagaaaaga ttttcgctaa atcctaattc taaagcgact
     3001 tctttcatgc ttttatctct ttggctagct aatgatttta tcctttcaaa cagtgtcata
     3061 ataatgttct ccaatcctaa acaaataaaa gtacacaaaa tgtaaattat actgttgaca
     3121 taagtataag tttgtattat tatttgtttg taagttaatt tgatagaaaa aaagcaaact
     3181 aaaaatacac cttatagcat taagtttggc gaccgattgc ataataaagg gtttgttgta
     3241 ggcttattta actatgttta tatagtatac gattgtatac tattagtcaa tagtttatac
     3301 gttttttcta tcattttgac ttacaaatct atatgtaagg ggagtgaata gatgtcaaat
     3361 ctagataacg gccgtgctgc aatcaagaag tttatgcgag aaaacggagt aactatggaa
     3421 gatttggcta ctgcatatgg atacacacgt gttcgaatgc agcaaattat tgatggccat
     3481 tggagtggac caaaagctaa taaaactatc ttagaaatca tccgtgatta cagaattcga
     3541 taggaggaat taatcaatgg aacaactagc tttagttaac ctctcagccc tcaaagcttc
     3601 tttggcagaa tctgagattg caaatgaagt gtgggataca aagcaagctg cggaatattt
     3661 gaaaaccacg acacgcacgc taactaaaga tgccgaatct ggaaagattc cagcagcgaa
     3721 agttggcaga gagtggaggt ttagtagtat cgctttgtat cagtatctaa aaggaggaaa
     3781 aacatgggca aattcaacag agcactagta ttcagcgcac cgctaatcat ctacgcttta
     3841 gggctttggg gaaatagaca agcgttgata ggaacgattg tttacatggt ttggattttt
     3901 atggggcttg atgaagctga agctgagtac agagcgaaaa agccaaccga gggggctgac
     3961 taaaaatgaa tagaaaagag aaactagaat gtatattact attactcagt ttaattcttt
     4021 cactaatttc tctattggga agtttgtatt tttgatatca acaattttgt ggatttgggt
     4081 tctatttaga gtaaatatca tttttgtttg gtgattaagg agtttattaa aattaatttt
     4141 ccctacttca aatgcaaggt aacaagatat agcactttta ggaggaatat ttagcggtac
     4201 agcttcagag tatattctat tatcatcgac tgatcctcct ttagcaatta atttcttaaa
     4261 tggagtagct gaccatctgc ggtttaagtt agcggatgaa agttctaaat taactaacat
     4321 agctggttct gttgaatagt tagaaatgat tacttttgta taaatcatat ctgcagcgaa
     4381 gtaagaagca ttaagttcaa cttctagttg agggcgcatt ccttttattt ttatccctgt
     4441 taaaaacgta ctcaatataa aacttattat tgacatccat tgaaaaattg ttaagtgaaa
     4501 aaaattcaaa ataaacacca ccagttttta actaaattat accaaaaagg agagaagaaa
     4561 taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc ttacaagtgg
     4621 cagaaagctt cgagaaaaaa catcaacatg ttttaagaga tattgatgca ctaaaaaaag
     4681 atgtgtccaa ttttggacag atgtttgtag aaggtaatga accagattca tatggcagaa
     4741 atcgacgagt tttcttcatt agtagagatg gatttttctt gctggctatg gggtttacag
     4801 gaaagaaagc tatctacttc aaacaaaaat acattgaagc attcaacgaa atggaagatg
     4861 ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat gatgatctat caattagaag
     4921 aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga attagcggac
     4981 aacaagagtt tgaaattaag caaaaaggaa atatgaaagt tatgggagtt ctagggggaa
     5041 aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat
     5101 ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg
     5161 ctgtttcatt tattgaaatg tggttgccag aaactgcgat ccgtatggaa attgatcaac
     5221 tgaacagaca acagagactt tttggtgatg acaatgaata gagctgaagc gctaagaata
     5281 gggacggcaa ttgctaattg ctggtggaaa tactataaac caatcatcct aagccaacaa
     5341 catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag
     5401 agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc
     5461 tagatcaata tttgattgat cctgattggg gcaagccgaa aattgaggaa acaagtggtc
     5521 gaaaaatcag acgaaatctt ttgacgaatg aagaactagc ttgggatcaa gatgatttag
     5581 gcaaccatgt aactatttgg gatcatgttt atcttatcca tctatcgaag cattcgaata
     5641 aacctgaata tatttacgtc atcgaagatg gcttgattga tgcgctagaa gagtacgaca
     5701 gagataactt gattgatatc tcttattacg gaccaggtaa gaaatacatt gctgaaatgg
     5761 aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt
     5821 aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg ggctcacgcg
     5881 tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtacaaacac
     5941 tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt
     6001 atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc
     6061 gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa
     6121 atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg actgggatta
     6181 tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacaaa gaaattaaac
     6241 atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat gaccaaaacc
     6301 ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga taacgttgaa
     6361 actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat agcagctaag
     6421 agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat tcatattcag
     6481 cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac catcaatgag gaagtgaaca
     6541 ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagtcatt gaaattcgtt
     6601 t
//
LOCUS       DAYYAO010000120         6334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000120 DAYYAO010000000
VERSION     DAYYAO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.125"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..1226)
                     /locus_tag="ACHARI_002592"
     CDS             complement(96..1226)
                     /locus_tag="ACHARI_002592"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8443593.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1286..1513)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1875..3077)
                     /locus_tag="ACHARI_002593"
     CDS             complement(1875..3077)
                     /locus_tag="ACHARI_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443594.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3137..3340)
                     /locus_tag="ACHARI_002594"
     CDS             complement(3137..3340)
                     /locus_tag="ACHARI_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8443595.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3482..4546)
                     /locus_tag="ACHARI_002595"
     CDS             complement(3482..4546)
                     /locus_tag="ACHARI_002595"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8443596.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4607..4714)
                     /locus_tag="ACHARI_002596"
     CDS             complement(4607..4714)
                     /locus_tag="ACHARI_002596"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443597.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4877..5689
                     /locus_tag="ACHARI_002597"
     CDS             4877..5689
                     /locus_tag="ACHARI_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443598.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6063..6254)
                     /locus_tag="ACHARI_002598"
     CDS             complement(6063..6254)
                     /locus_tag="ACHARI_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443599.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1886 a         1300 c         1000 g         2148 t
ORIGIN      
        1 taaataatat aaaaggtaaa taaaaaagag tggatttctc cactccatga aaagccgact
       61 ttttgggacg cttttttttg ccaagcctca catttttata gccaaacttc ttttgcgact
      121 tgctgcaata attggatttt cttccattgt tcttcttctg ttaaatggtt tccttcttct
      181 gttgaagcaa acccacattg aggactgata cttagatttt ccaatggtac aaattgttgt
      241 gcttctttta ttcgttcttt tattttctcc gtatcttcca aatctggaaa cttagaagta
      301 actaatccta aaacaacttt actttctttg ttactccaat gtcttaaagg agtaaaatct
      361 cctgctcggt cagaatcgta ttccaaaaag aaaccatcat aatttgttga aaacaactct
      421 tctgcaatgg gttcatatcc accggaaaac agccaagaag aagcgtgatt cccgcggcag
      481 atatgtgttg tgatcgtcaa atcttctggt ttgtcttcca gtgcgttatt gataatctct
      541 gtacaaatat gggccatttc tttcggatca attccttttt gaagaagtgc ttctctttta
      601 gtttgatcag ctagaaacgc ccaatttgta tcatcgaatt gcaggtaacg gcaacctttt
      661 tcataaaaag catgaatcgc tgctttgtaa gtagaggaca agtcctcata aaaatcagat
      721 agatcaggat aaatctcctt gatttttgaa gaaccatcat tagataggat ctcttgtcgt
      781 aaaatcatcg tgggactagg gatcgttgct tttgcaacaa aagtatcatc tgtttccact
      841 acttctttca gaaaagcaaa atcttttaaa aatgggtgat ttggattgaa agataattta
      901 tctactaccc gaatattata agctggtgct gcttttccgt ggaatgtttg attatatcca
      961 gtttctggaa catagccttc gataccattt agttcttcta aaaaatcaag atgccaaaat
     1021 ccacgccgaa attcaccatc tgtaattcct ttgatgccga ctgctttttg gtcttccact
     1081 aattttttta tttcctggtc ttcgattgca cgaagttctt cttttgaaag taagccttct
     1141 tgaacttttt ttcttgcttc tttgatcgat tgcgggcgta agaaacttcc gacatgatct
     1201 actgtaaatg gtctattttt gctcatttca ctcactcttt tctttgtttt ttgtaaaaaa
     1261 aaatcctttt actagcaaat actagtaaaa ggacgattat actcgtggta ccaccttttt
     1321 tcagaaaaaa cattctttct ctcagcctga caataaaatc agtggtcttg gtaacgaaag
     1381 actgttcgtt tttgcctacc tttcgacaaa aaatcctcta aggccatttt cgacagtcaa
     1441 cttctagctt tttacaccaa ccaaagcctc tcttgaacca ttttctgtct actttccttc
     1501 tcatcggata ctattagaat aaaattagaa tagcatgttt gaaagaaaag tcaataggga
     1561 ataagaacaa acaaaaacta atttgttatt cttagtaatt tgtttgtgga atacaataaa
     1621 gcttttctaa tatgataatt gttcagtcgt cctttcatct tagatcactt ccttaaaatg
     1681 atcatacccg tttcaaccac ataaaaaagg acttaatttt gaaaaatttc ccatcaaaac
     1741 taagtccttt ttattatagt tatgcaaatt tgcttatttt ctcgctattt ccgatacatc
     1801 ttgaacagga gcaattcgac tgaaaacact ggtgcaaaag catttatttg tcttatggta
     1861 gaaccgtgga aaactcagac tggtaataaa ttgtcatttt ttgaaagaat agcgccctct
     1921 tgaacattat caatcgcact taccacttgt tgatctgact ttgcatataa gtgaccgtaa
     1981 gttcctaaag tgatttgaat attcgcatgg cccaatcgtt cttgaataga tagcggttga
     2041 atattctgct caattagaaa tgcaacatga gaatgtcgta gcgcgtgaag cttaatcggt
     2101 ttgacacccg cttttttagc agcttctttt aaccagttcg caaaagaata aggtggcgta
     2161 aatgttccgt ccaactgagc aacatattca aaatttccaa tacgtttttg caagtcatac
     2221 cactgtctga gttttaagga aagcttcttc cccaacccaa tcgttcgata agcacattta
     2281 gttttgggag tcgaaaagta ccaatcatct tttgtattat aaatcaatgt cttctcaatc
     2341 gttatcgtgt ttttctcaaa atctacattc gaccaagaca aagcttcaag ttctccaatc
     2401 cgaatgccag tataaaagag catttcatac acaatcttcc gatggtgggt ttgaatctta
     2461 tcatccttca attcattcat cactttttga aactcactgt gttcccaaaa ctcaactttc
     2521 ttattctcag aaggaagcga agaaacatct tcaacgggat ttttagcaat caccccctct
     2581 ttaacagccc gatccaatac aatttttagg tgccctcgaa tccgattgat ataagatttt
     2641 gataacgttt cgccattaac ttttttccgt gaattacgtg actctgtctg gcttagttca
     2701 aataaccaat cctcaatatc gtgacttgta atatctcgaa cacgaaaatg atagaaaaac
     2761 tgcatacgct tttcgaaaat gaattgcgca ttctcatatg tttgactttt gacctgtctc
     2821 tcgtaccatt ttacaaacct atccttgtaa aactcttcga aagtcatttg attggtagaa
     2881 cacagttgat taggaattgt cttgttaaaa tcatgtgttt ccatgtactt gtcataagca
     2941 attttggcat ctttttgtga agcataacct cgtttagtct tctgaacctt tcttccattt
     3001 tcatctatac ctagatacgc tcggaagaac catttttttg tctttttatc ttggtaaata
     3061 tttttaattt ttggcatcgt tccattcctc cctgtacttg agaaaagcta ctttaatagt
     3121 accgcgcttg tactgtttat tccgcaactt gaatgtgaaa aagatgttca attactcggg
     3181 atggcaccac accaacttgg cgattattat aaaaatcaat accttcaatc cttgcaagat
     3241 atgccttgct ctctttaatc attcgtgcag cttgactagg tttaaagccc atctctatca
     3301 aaattgcacg atttaccatg aaatcacatt ttgtttccat tctctcacct cctgattctt
     3361 tactcctttt aaaataatcc tctttataat ctattgtttc tcattcgatg gttgtgttat
     3421 actcgtcttg tcgaggcgga gtacgttgca caacttgttg tgtaagcagt actttgcttt
     3481 tttagtagct tgttttctca tttgatatac tttgtaattt tcgttcataa tactcaacct
     3541 cctcctgata atactccggg tctgccttca tattttggag aagtttgatc tgtcttggat
     3601 tcatttctcc ttgttcaata atcaaaggca acaaggaaga atgaaacgtt tgatcgacct
     3661 gtgcaataaa aactaatgat ggtgaaacac catgaattaa ccaactcata gttttctcaa
     3721 aactttgagg aattgtatct aaagaaatgg tgattgcgcc ataattgcta ataaaggctg
     3781 cccatttggc atcaactttt gcacctagat catttggatc acggtcataa aaacaaattg
     3841 ctccattaag taactcaaag aacagtttgg ttaaatcatg ggtttttgaa ataatctttg
     3901 ccaagctttg cgctttctct tttcgatacc gtagttcaaa gcgattcttt acttttgcct
     3961 cttctaaagg aattcctcgt cgcttccgtt gttcatggtc cttctgataa aagcaaaaac
     4021 gacactgact cttctttgag cctaaataca aggtctcacc atttgattca ttgtttatga
     4081 tatccgtacc gccatgagat tcactcactt gaaaagtggt ccacacatgg cctagtatta
     4141 ccttatgctt aagttctgga atgctgagac tccccacaaa atcatctaaa gtaaaatcaa
     4201 tccgtgtaaa gttcccctga taatctaata ccttacgaaa aaactgttgc caatttgttt
     4261 ttcgtgtttt tagccatctt gctaagtgtt tacagccttg acctgaaaac tccatcacag
     4321 ttcctttaac cgcatcgtca atggaatacc gaatcgtgat tacttgattc cacacgtatt
     4381 ggccaatata gccctttggc gcataatcca gttcctcaaa acgttcaatg ggcatttcaa
     4441 taatctcttc aattagcttt aaatgttgat tcgagaaaaa gtgaatggtt agataatcaa
     4501 tcaaaacacg gttaactgga acgacctctt cttgcaaact atccaacaca agattcaatt
     4561 tttcttcact taattgtctc gttccttttt ctactttcga taataactat ctcgaaatcc
     4621 ccactttttc tgctaaatat cgttgtgtcc accccctttc ttctcgagaa actcgaatgg
     4681 cttcaccgag atcatgttga ttaacttggt tcatcgacaa acctccttct gctatttact
     4741 tgtcaaagaa cctattgaac ttattttaag tacattttta ttgtacgcct gtatttattt
     4801 taagtcaata tgttttgact ttttttaagt acacacgtat actaaattta agaacttatc
     4861 ctgaggagga gttttcatgg agatagacaa gcaagcagtc ggcaatcgaa ttcggcagat
     4921 tcgccaagaa ttaaagttga gtatggagaa atttggcaaa ctaattggtg atttaccgcg
     4981 aagtacagtc aataactggg aacgtgggat caaccttcca aaaaccgaaa cccttcacca
     5041 aatcgcagag ataggtcatg tcaccaatga atatcttttg tatggtgatc aagagaatca
     5101 atacatttta gaaatgctcc aaaaaaaggc aggtaagctt gatcctaaga ttgaaggtct
     5161 catagtagac gaaatcaaac acgctgacct tgttagtgaa aaagaaatgg atcgaatgat
     5221 tgaatttttt attacaaatc tcattccacc aactgaacaa gaccagttta ctttccaatg
     5281 tatcgatgaa aaaaagcagc tttatcttgg ttcaactaat tttggtaagg aagcccagct
     5341 ctatttgcaa catgataatc aaaatcatat tctgcacatg atgcccttta ccttctccaa
     5401 ctttagtgtg gatcgcatac tcgtctattt agcaaatcaa aaatcctatt cctattttgg
     5461 gaaacattta cccaaggaac taggtgagaa agccatcctt ctatactcaa ttaatcaatt
     5521 gaccagcgat gtgcgaattg ccccgttggt ctattcagaa gaaaccgctt cttatcagta
     5581 cacagaggac aatcaatatc tactagaaca gcaatatctt tatctaccat tcgtgatgga
     5641 agtcgagaaa gaacgcctgt taaatgcagc gtatccatca tcaaaatgag gtgacaaaat
     5701 tgataagcac gtgacaaaga aaatactgct aggacactcc ccaatgggta gttgtcacag
     5761 tttcctgttc tttgttagcc ccctgttaga accgggggct ttattcctgc agtccggcta
     5821 cgccttcctg caatagacaa aactccagat cgggtaattc gcttgtggcg aaacccgacc
     5881 tggagtttgt ctctgtattt tgaaccctaa aaattaatca aacagactga tattctcata
     5941 atcagcgcga aataagccat taagtacagt tttcctacct atttttgata acccgctggt
     6001 tagttaatat tccttttccg agagagataa atgattggtt aaatctatat acatctcaac
     6061 ctttaaaaat gttttcgaaa tacttcatca gtacatgtca tacaaattac cagtgcattc
     6121 tcaaattcta gttctttaat tgatttcagt tgaggagcag ttacttttgg cggaaatttc
     6181 catttttgtc caaacctctt cccccgatga aatttctttg ttgcaattat tgcaatgaaa
     6241 aatttttgtt tcaaaaatat acataattac ttcctccaat aaaaactatc tacctctcaa
     6301 attatttttc tctaataaga aaaacagaaa aggt
//
LOCUS       DAYYAO010000121         6286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000121 DAYYAO010000000
VERSION     DAYYAO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.126"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..362)
                     /locus_tag="ACHARI_002599"
     CDS             complement(108..362)
                     /locus_tag="ACHARI_002599"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8443600.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(766..1548)
                     /locus_tag="ACHARI_002600"
     CDS             complement(766..1548)
                     /locus_tag="ACHARI_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443601.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1613..2449)
                     /locus_tag="ACHARI_002601"
     CDS             complement(1613..2449)
                     /locus_tag="ACHARI_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8443602.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2467..3876)
                     /locus_tag="ACHARI_002602"
     CDS             complement(2467..3876)
                     /locus_tag="ACHARI_002602"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8443603.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3890..5713)
                     /locus_tag="ACHARI_002603"
     CDS             complement(3890..5713)
                     /locus_tag="ACHARI_002603"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8443604.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1935 a         1351 c         1013 g         1987 t
ORIGIN      
        1 tttattctta taaaaataat agacacttct agggatttct aataaccgac ataacaaaga
       61 aacagtgtaa tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga
      121 gagaatatgg ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct
      181 tttctcagtt cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa
      241 ttcttccatt tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca
      301 gaacgtccag tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc
      361 ataaaaattc atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc
      421 taagatttga tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat
      481 tacatatgat tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc
      541 ttaaaaattt tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg
      601 tttaatgaaa ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc
      661 cgaaaaaaaa tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat
      721 ataacaacgg ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt
      781 tttaaatact tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt
      841 tttcccattt tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt
      901 actgatacct gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc
      961 tgtatcagta tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat
     1021 agctgtgtat gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac
     1081 ttctgtctca ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc
     1141 ttctgtctca ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc
     1201 tttagtgtgc atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct
     1261 ttcaatagag acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa
     1321 aagcattttg ttgtttacat ctacataata atcctttgac tctacttgag accttcctat
     1381 acggaataca gctttactgt ctgatatctc tataccagca tccttattat taatctgcca
     1441 atctccaagt agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact
     1501 ggtagaggtc gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc
     1561 cttagcacaa ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca
     1621 cccttgaaat atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct
     1681 cttcttgaac gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct
     1741 tcactgaagc cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg
     1801 caataaaaaa ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct
     1861 ttttttcggg aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga
     1921 gcatggtttc ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt
     1981 ttccagcctc ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat
     2041 agcagccgat ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt
     2101 gaaaaacaat gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa
     2161 aaaatagact tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct
     2221 tgacgcaaat aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta
     2281 gagcatcaga aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat
     2341 cgcctttctt tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct
     2401 tatctaagca aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg
     2461 ttctctctaa gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt
     2521 ttttattcgt tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata
     2581 acgcttattg aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc
     2641 aaaaacatct acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata
     2701 atcaattcga tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata
     2761 gccattttca gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa
     2821 tcgtagtcca gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc
     2881 agctgcccgg tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga
     2941 aacatctgat ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc
     3001 aataaaatcg ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata
     3061 taatccacga tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac
     3121 atctaagaaa gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg
     3181 actggaggta gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag
     3241 attcaactga tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat
     3301 ttgaaagagt tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg
     3361 atgaaggatc atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata
     3421 ctcagtaagc aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca
     3481 accgccctgc tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc
     3541 gattccctga ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt
     3601 ggttttatta ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt
     3661 taagcctaat tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc
     3721 aacttcaaaa ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt
     3781 cccatcctcc tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa
     3841 cttctctgga aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt
     3901 gcatcaatgg ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt
     3961 cactgttggt cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga
     4021 aatcggcgtt gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt
     4081 caaagggttc cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac
     4141 cttctttaga acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct
     4201 caacaggtga ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc
     4261 ccttagaaaa gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat
     4321 tttcaacaat gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa
     4381 atccaattag ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg
     4441 cgtgaacaat attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg
     4501 aaacaaaggc atatctgact aaaccgctca agccagcata caaaccagca attgcaccac
     4561 caatcatgga agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct
     4621 cagtaattcc cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt
     4681 ttttagtttt cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca
     4741 ttcctggccc attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg
     4801 tcattgccca agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg
     4861 gaattaacca agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca
     4921 gcaacgttcc aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca
     4981 gcatcggctt agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta
     5041 cataggacat gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag
     5101 ctaataccgg aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat
     5161 ttggatgaag caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta
     5221 tggctgcccc ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat
     5281 tcaaaatgat gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca
     5341 aaatggcttt taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag
     5401 aaatgacact aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt
     5461 taattgaagt tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa
     5521 tcaccacttg gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta
     5581 aggctttcgt atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat
     5641 gggttaagct gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt
     5701 ctctgtaatc cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc
     5761 ccaatcaaca ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca
     5821 ataacgttat tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa
     5881 tttaattttt tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa
     5941 ctagggtata tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca
     6001 gtaaaaccat tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg
     6061 catataacgg atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt
     6121 gagtaaagga tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt
     6181 ttttggtaaa aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg
     6241 atagaatgat ttgggctagt attcttaaaa attaactagc aaatgt
//
LOCUS       DAYYAO010000122         6230 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000122 DAYYAO010000000
VERSION     DAYYAO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.127"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACHARI_002604"
     CDS             133..633
                     /locus_tag="ACHARI_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8443605.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACHARI_002605"
     CDS             730..2577
                     /locus_tag="ACHARI_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8443606.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACHARI_002606"
     CDS             2580..3476
                     /locus_tag="ACHARI_002606"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8443607.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACHARI_002607"
     CDS             3526..3915
                     /locus_tag="ACHARI_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8443608.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="ACHARI_002608"
     CDS             3902..>6230
                     /locus_tag="ACHARI_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8443609.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAYYAO010000123         6110 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000123 DAYYAO010000000
VERSION     DAYYAO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.128"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(322..807)
                     /locus_tag="ACHARI_002609"
     CDS             complement(322..807)
                     /locus_tag="ACHARI_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443610.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(946..1161)
                     /locus_tag="ACHARI_002610"
     CDS             complement(946..1161)
                     /locus_tag="ACHARI_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8443611.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1385..2185
                     /locus_tag="ACHARI_002611"
     CDS             1385..2185
                     /locus_tag="ACHARI_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8443612.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2204..4072
                     /locus_tag="ACHARI_002612"
     CDS             2204..4072
                     /locus_tag="ACHARI_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8443613.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4065..4556
                     /locus_tag="ACHARI_002613"
     CDS             4065..4556
                     /locus_tag="ACHARI_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8443614.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4544..5098
                     /locus_tag="ACHARI_002614"
     CDS             4544..5098
                     /locus_tag="ACHARI_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8443615.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5116..>6110
                     /locus_tag="ACHARI_002615"
     CDS             5116..>6110
                     /locus_tag="ACHARI_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8443616.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2161 a          968 c         1224 g         1757 t
ORIGIN      
        1 tttgaaaaag ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa
       61 ttcttctatc caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt
      121 aactcagttg accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa
      181 gtgagtatat tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat
      241 ttatactccg tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta
      301 aatccgtata atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga
      361 tccttcgggt gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg
      421 tttgctggaa gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat
      481 atcgtcactt tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt
      541 ttctaatgga agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa
      601 cgactgaata cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat
      661 ggaagtagta gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga
      721 ttctttaaca gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa
      781 caagcctgcg taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc
      841 agtatatcac ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa
      901 gggaaggttc atgaagataa taactttttt aataattata gctttctaat atctttcatc
      961 aacttttcta gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac
     1021 gatataaatg atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc
     1081 aagttcgaac aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc
     1141 aatagcatct aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat
     1201 caaaaagaga aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat
     1261 gaaaattttt tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg
     1321 tcttaatttt ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc
     1381 acatatgtcg gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc
     1441 aggaattgga agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt
     1501 tgatgtaact gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc
     1561 ttctaaaacg gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga
     1621 tggtctagtc aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc
     1681 aaaaagcaag tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa
     1741 aagtgtatat ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt
     1801 gatcgtgaat ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc
     1861 tgctacaaaa gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag
     1921 gaatgttcgt gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca
     1981 agaatatgaa gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg
     2041 ttacagtcga acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga
     2101 tctagtctgc tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat
     2161 ttcaggaggt aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag
     2221 atggtaccaa ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca
     2281 aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga
     2341 aattgccggt attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga
     2401 ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc
     2461 ggcatccaaa agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg
     2521 tatggatgat ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa
     2581 gacattaaaa gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg
     2641 actaagaata ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc
     2701 ctacttttca gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt
     2761 tcagaagaag acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat
     2821 ttctttgaat agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg
     2881 gtgttttgaa tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga
     2941 actaactttg agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac
     3001 aagtataacg atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat
     3061 gatgcgtatt cgacgattag atctcattga tctggatgag tccttcttat atcaagagat
     3121 gaaacagcac ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt
     3181 attttatggt gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat
     3241 acaagcatta gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac
     3301 gttgtatttg gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc
     3361 cattgttcat acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat
     3421 attggggagg gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct
     3481 aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc
     3541 ggtgatccat ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc
     3601 aaaaaaaaag cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga
     3661 aaaaaagacg tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta
     3721 tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt
     3781 taacaaagaa tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc
     3841 agtagggcta ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt
     3901 atttgccata cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga
     3961 tatcctattt gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca
     4021 agaatcattt ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc
     4081 tattcatagt tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac
     4141 taaagcattt caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta
     4201 aacgacctgg gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc
     4261 aagttttaga agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac
     4321 ctgtctatgc gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg
     4381 aagatcgttt gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg
     4441 caggaaatga aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc
     4501 ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa
     4561 atttgctgag ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat
     4621 gacaggaatc gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt
     4681 tttaggtgaa gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg
     4741 ttaccttgtg ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat
     4801 ggctcgtttc cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg
     4861 tcatacgagc aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg
     4921 gattgctcaa gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt
     4981 gcttgtggga ctgctgatga actttattgg tggttggata actgatttta ctactggatt
     5041 tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag
     5101 aaaggagagt tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga
     5161 ggtcctttga ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga
     5221 ggtgagaggc caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat
     5281 gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg
     5341 actcttagat gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca
     5401 ggaaaaagtg gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg
     5461 ttagaacaaa tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca
     5521 tctccagata aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag
     5581 cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat
     5641 caatcagcaa gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc
     5701 gtttcccttt tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt
     5761 atggcacctt tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg
     5821 ccatttttat ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg
     5881 atcggtgtag agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt
     5941 gctatcaata ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca
     6001 aaagctgaga cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc
     6061 atcccacgag ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAYYAO010000124         6068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000124 DAYYAO010000000
VERSION     DAYYAO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.129"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACHARI_002616"
     CDS             832..1566
                     /locus_tag="ACHARI_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8443617.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACHARI_002617"
     CDS             1582..2751
                     /locus_tag="ACHARI_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8443618.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACHARI_002618"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACHARI_002618"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8443619.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACHARI_002619"
     CDS             3841..4767
                     /locus_tag="ACHARI_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8443620.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACHARI_002620"
     CDS             4786..6036
                     /locus_tag="ACHARI_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8443621.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1247 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAYYAO010000125         6020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000125 DAYYAO010000000
VERSION     DAYYAO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.130"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..362)
                     /locus_tag="ACHARI_002621"
     CDS             complement(60..362)
                     /locus_tag="ACHARI_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8443622.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACHARI_002622"
     tmRNA           complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACHARI_002622"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1119..1892)
                     /locus_tag="ACHARI_002623"
     CDS             complement(1119..1892)
                     /locus_tag="ACHARI_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8443623.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2036..2386
                     /locus_tag="ACHARI_002624"
     CDS             2036..2386
                     /locus_tag="ACHARI_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8443624.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2367..3083
                     /locus_tag="ACHARI_002625"
     CDS             2367..3083
                     /locus_tag="ACHARI_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8443625.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3083..4531
                     /locus_tag="ACHARI_002626"
     CDS             3083..4531
                     /locus_tag="ACHARI_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8443626.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4602..5111)
                     /locus_tag="ACHARI_002627"
     CDS             complement(4602..5111)
                     /locus_tag="ACHARI_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443627.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5101..5825)
                     /locus_tag="ACHARI_002628"
                     /pseudo
     CDS             complement(5101..5825)
                     /locus_tag="ACHARI_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2045 a         1050 c         1170 g         1755 t
ORIGIN      
        1 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
       61 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      121 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      181 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      241 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      301 ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac
      361 ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc
      421 attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa
      481 tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa
      541 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
      601 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      661 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      721 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      781 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      841 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      901 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      961 gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
     1021 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
     1081 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
     1141 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
     1201 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
     1261 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
     1321 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
     1381 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
     1441 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
     1501 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1561 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1621 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1681 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1741 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1801 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1861 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1921 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1981 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     2041 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     2101 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     2161 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     2221 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     2281 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     2341 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     2401 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     2461 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     2521 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2581 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2641 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2701 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2761 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2821 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2881 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2941 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     3001 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     3061 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     3121 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     3181 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     3241 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     3301 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     3361 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     3421 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     3481 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3541 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3601 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3661 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3721 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3781 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3841 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3901 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3961 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     4021 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     4081 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     4141 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     4201 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     4261 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     4321 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     4381 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     4441 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     4501 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4561 gatcttctat accgcttgtt ttttactata aaatgattga tttagcccgc gggctctaaa
     4621 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4681 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4741 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4801 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4861 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4921 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4981 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     5041 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     5101 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa agtgaaaggg
     5161 aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta
     5221 agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc
     5281 atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta
     5341 gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt
     5401 acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa
     5461 ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc
     5521 ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat
     5581 aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct
     5641 gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat
     5701 tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa
     5761 caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt
     5821 ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc
     5881 ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa
     5941 gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga
     6001 ggagcagcga caataatttt
//
LOCUS       DAYYAO010000126         5954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000126 DAYYAO010000000
VERSION     DAYYAO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.131"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHARI_002629"
     CDS             <1..813
                     /locus_tag="ACHARI_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8443628.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHARI_002630"
     CDS             786..1229
                     /locus_tag="ACHARI_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443629.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACHARI_002631"
     CDS             2399..3367
                     /locus_tag="ACHARI_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8443630.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACHARI_002632"
     CDS             3384..3908
                     /locus_tag="ACHARI_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8443631.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="ACHARI_002633"
     CDS             4284..4565
                     /locus_tag="ACHARI_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443632.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="ACHARI_002634"
     CDS             4567..4983
                     /locus_tag="ACHARI_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443633.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="ACHARI_002635"
     CDS             5613..>5954
                     /locus_tag="ACHARI_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8443634.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2259 a         1018 c          965 g         1712 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggcatagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAYYAO010000127         5903 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000127 DAYYAO010000000
VERSION     DAYYAO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.132"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(452..955)
                     /locus_tag="ACHARI_002636"
     CDS             complement(452..955)
                     /locus_tag="ACHARI_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8443635.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1314..2339
                     /locus_tag="ACHARI_002637"
     CDS             1314..2339
                     /locus_tag="ACHARI_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8443636.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2662..3540
                     /locus_tag="ACHARI_002638"
     CDS             2662..3540
                     /locus_tag="ACHARI_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8443637.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4016..4132
                     /locus_tag="ACHARI_002639"
     CDS             4016..4132
                     /locus_tag="ACHARI_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8443638.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5060..5398
                     /locus_tag="ACHARI_002640"
     CDS             5060..5398
                     /locus_tag="ACHARI_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443639.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5492..5722
                     /locus_tag="ACHARI_002641"
     CDS             5492..5722
                     /locus_tag="ACHARI_002641"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8443640.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2002 a          884 c         1083 g         1934 t
ORIGIN      
        1 gatttctcta ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta
       61 ggatcaggca gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac
      121 catagaaaat ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct
      181 gagtagggta taaattaaga cggaattcct ttctagattg agatattgac taggaagaat
      241 gttaagattt taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata
      301 gcctgtcagt ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa
      361 aatagatttt tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg
      421 tgtctattac ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa
      481 ataaaaaagc tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt
      541 ctaacttatt tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc
      601 ctcctgttgt attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt
      661 gaatcaattc tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa
      721 gtctcttgaa attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag
      781 tggtctcacg taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga
      841 cattgctgta agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca
      901 accataatag aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca
      961 tctcctccat ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa
     1021 gtttaaggac caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg
     1081 gtttctttaa caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc
     1141 aaaacgactc ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata
     1201 ctaagtttat gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt
     1261 ggcatttcat cttggttcag ctttttttcc aattactagg aaggagggat agattgttca
     1321 agacttattt tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc
     1381 tacttatctt tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt
     1441 tttcatcatt agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc
     1501 tgaaacgtat gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat
     1561 cgacttatgt tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat
     1621 tcgtgttgcg attattccat atttctaaac caggtggctt gttttttgcg atgctttctg
     1681 ccatgggaac gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt
     1741 ttatgggtgt cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc
     1801 ctgagcagac aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga
     1861 tcattgattc tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct
     1921 tgaatctgca taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa
     1981 acttggatgc gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat
     2041 gtgtgtcggc attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca
     2101 cttttctata tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt
     2161 ttcttaatcc gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt
     2221 tgatcgaata tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt
     2281 gggtaatgac ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga
     2341 aggacaaata tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca
     2401 aggtgttcaa aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga
     2461 actttcaaaa aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa
     2521 taaactttcc tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat
     2581 agaaagttta agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc
     2641 ggataatcag tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag
     2701 aaaataagca atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata
     2761 gagcaaatga ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat
     2821 gaactaatgg atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa
     2881 gaggaacctt ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa
     2941 atttatcaac cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact
     3001 agatttattc atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg
     3061 atttggcatt acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca
     3121 tattttccga aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat
     3181 attccttccg atgcagattt atctcagaat aagataagag atagttacga aagtgccatt
     3241 cgtttttttg aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg
     3301 ttactatcac ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca
     3361 gccgattatc gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt
     3421 ggacgagtag atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca
     3481 aaagagcaat tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa
     3541 gtttagtact tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct
     3601 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa
     3661 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     3721 cttctgtccc agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa
     3781 ctagctataa caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa
     3841 acgtaataat gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt
     3901 tttaacaagt tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt
     3961 ttcgatttgt gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga
     4021 aaaaacagat atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa
     4081 acgtgcgctg aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg
     4141 aaacttaatt aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt
     4201 gtgtaaggtt tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt
     4261 agaatctttc ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga
     4321 accacaaagc acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca
     4381 acctcttatt ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt
     4441 gaaacatttt tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt
     4501 tcatgaaata tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat
     4561 aagcagttac gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca
     4621 aaaaaatcag tgattcgtat ctctttctat ttgtcaaaaa agttaacttt aaaaagtaac
     4681 gccgaaaaaa tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga
     4741 ataataagtt ttggttttca gataatagtc gataagttgt gtattggaat tttagataca
     4801 gtaataaagt atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa
     4861 attaatggtt aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt
     4921 gtgatactag actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa
     4981 atgaaaaaga agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt
     5041 tagccaagta acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt
     5101 tcgtgctaca gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc
     5161 tagtgagcca attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt
     5221 tatggtggta aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat
     5281 tgaaaaggtg agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat
     5341 cattttaacg gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac
     5401 actagtgaaa tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa
     5461 atcgatcatc aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat
     5521 ttattacgtg aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag
     5581 tcgctactca attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa
     5641 aactgttttt tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga
     5701 ttatgttagt atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg
     5761 ttattgtttt ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt
     5821 atccagagta ttttatttta acttttgtat atttagtaag tagctacaca cttctatata
     5881 aattcagaag ttcttctaga gtt
//
LOCUS       DAYYAO010000128         5769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000128 DAYYAO010000000
VERSION     DAYYAO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.133"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHARI_002642"
     CDS             complement(<1..300)
                     /locus_tag="ACHARI_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8443641.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHARI_002643"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHARI_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHARI_002644"
     CDS             complement(1014..1304)
                     /locus_tag="ACHARI_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443642.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHARI_002645"
     CDS             1706..1942
                     /locus_tag="ACHARI_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443643.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHARI_002646"
     CDS             1949..3901
                     /locus_tag="ACHARI_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8443644.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHARI_002647"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHARI_002647"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8443645.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACHARI_002648"
     CDS             complement(4797..5489)
                     /locus_tag="ACHARI_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8443646.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DAYYAO010000129         5666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000129 DAYYAO010000000
VERSION     DAYYAO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.134"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..454
                     /locus_tag="ACHARI_002649"
     CDS             <1..454
                     /locus_tag="ACHARI_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM8443647.1"
                     /translation="KELNYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQL
                     KANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSL
                     NDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            735..1955
                     /locus_tag="ACHARI_002650"
     CDS             735..1955
                     /locus_tag="ACHARI_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8443648.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1952..4672
                     /locus_tag="ACHARI_002651"
     CDS             1952..4672
                     /locus_tag="ACHARI_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8443649.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4656..5600
                     /locus_tag="ACHARI_002652"
     CDS             4656..5600
                     /locus_tag="ACHARI_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8443650.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2107 a          908 c         1113 g         1538 t
ORIGIN      
        1 caaagagcta aactatgagc ttgagtatga aaaatctatt tgttcaatcc cttataataa
       61 tattgaggaa attatagaaa aggatgctat cagcatttac aaatttttta tttttattcc
      121 taatcgtcaa ttgaaagcta atatatttaa ccatttgaaa aagatgctaa atgtgtctgt
      181 tactgaatcg gcacctgtaa atattgaaat cgtacctcaa aatgtatcta aaggtgtggt
      241 gtttgatcgt ttggaatcaa tctacaattt aaagaaacct ttgagaatcg cgatcggaga
      301 tagtttaaat gacttatcta tgtttgaaag tgcagatatt agttttgcaa tgggaaatag
      361 tcatcaagaa atcatgaaaa aagctgatta tgtcactgtt tcaaatgata aagacggagt
      421 atctcaagca tttgaaagaa ttcttaccat ataaactaat taaggtagta tagaacatag
      481 aattctaaat acggaaaaat ggagcataaa ttccagtaca aatagtactt cttcagtgtc
      541 aaacgaagcg aatataacat agatatttag gattgtgaca aaaatcatgt cacaatcttt
      601 ttttcgaata aacttatttt ttgaagtcgg ccatttatcg taatttcttt gcataatacg
      661 aacaaatttt cggttattga tgaaaaaaaa cattgaaaat ggtatgataa aagaaaaaaa
      721 ggaggaaatt tttaatggtg aaatttatcc atgcggcaga tttacatatg gatcggtcat
      781 ttgaaggact gacaactctt gataaaactg tacaggaaaa actattgaag acgaatctga
      841 ccgttttatc aaatatcatt gaacaggcaa taataaataa cgttgatttt gttttacttg
      901 caggagataa ttttcatcaa aatcgtcctt cattaaaaat acagaaacat ttttcagaac
      961 agatgaaacg gttagaaaag aaccatatac ctgtatttat aatattcgga aatcatgatt
     1021 tctatcaaaa agagcggtat tggttctcct ttcctaaaaa tgtccattta tttacatccg
     1081 aaacggtaga gacaaaaaaa ataacgataa aaagtggaga aactgtctct ctcagtggat
     1141 ttagttatcg tcaaccgtgg attcaaaaag acaaagtgat ggaatttcct tctagagaac
     1201 tcacagatta ccatattggg ttgtaccacg gggaacctgg cgtctctcaa aaaggaaact
     1261 atgctccttt ccaaccctca aagatgcaag aaaagggata cgattattgg gctttagggc
     1321 atatccatgt gccaacggtc ttaaatgaaa agcaaacaat tgtttatcca ggtgcgcctc
     1381 aaggtcatac taaaaaggaa caagcttcta catctattct actagttgaa ttaagtaaag
     1441 gaagttgcca aatacatccg attaaagtag cagaggtcta ttggaagaca aaggaaatat
     1501 cattgaggaa agcaagaacg acaaaagagg tcattgttca cattcgtcag cagctttcaa
     1561 aagtagagga tggatttagt ttaattgaga taaaattaaa agattatgac catctcaata
     1621 cagatgtttt ggaacggata caatcaggag agctattaga ctatttgtta gaggaattct
     1681 cagataggat aaatgatttc tttatttggc gtatttctct catagagaat actttttata
     1741 cgaagacccc acttgctgct tctgctaaat tgatggaaca gttatttcaa tactatcaga
     1801 cacctcaaga tttccaacaa attttaaatg aagtatatag tcatcaagaa gcggctagaa
     1861 tattgagcga actgccggaa tatcaagaag aaacacttga aaaagccaag cagctgctta
     1921 atcaagattt tttatttgag gaggatcagg aatgaagatt ttaaggatcg agattgctgc
     1981 ttttggaaaa tggcgtcaaa aaagttttga cttttattca ggcaatcagc tgatctatgg
     2041 gggaaacgaa gcaggaaaat caacgattta tcaatttatt caagcaatat tatttggttt
     2101 cccttcaaaa ggccgtaaaa aaaaggatta tacaccaaaa gacggctcag cctatggcgg
     2161 caaaatatgg cttaaacatc ctgtatatgg tgaatttgct gtcgagcgct acaagcagca
     2221 aaatcgcgga aaatcgaagg tgtggctggg ggatcaagtg ggaagtgatg agttactaga
     2281 aaaactgatc gcacctttga cgaaagagtt attccagcag gtatttactt tccagcaaga
     2341 gcagttgatg gaactagatc atttgagaga aaaagagcta catgatgcgc tgatttcttt
     2401 aggcatcacg ggcagttctc aagtattcca aaagcggcaa gaatattatc agaaagcaca
     2461 acaattattc aagaaaaaag gccaaaaatt actaatcaat caaaaattaa atgaatggaa
     2521 agagctgcaa gaaaaaattc atcaaaaaca agaacaggaa gcttattttg gcaagttgat
     2581 ccaacaagaa caggaatacg agtcgcaaca gcagcagtta ttggcacaat taaaagaggc
     2641 gaacaaacaa ttttcattag ccagacagca agaacttaat ttttctttat atgaagaatg
     2701 gcaatctctc aaaaaaaaca gcactacagg tcttcctgat gagcaacttc ttttagatct
     2761 ggagtctttc tccaaacggt atcagcaaac aaatgagcgt ttggaagaat tagaagcaac
     2821 actggaaaag catagcggga tggatcagca gtcagccaga tattattttt atttagagca
     2881 ggagcaggaa ctaaaagatc tccagcaact tcaatttaca gcggaacaat taaacgaaga
     2941 atggcaaaga ttacaaattg agactagaga aacaaattca gaattgaaga ttttggaaca
     3001 aaggtggcat tggcagaaaa atccgttacc ggaagaattt ttatatgaag aagaatggaa
     3061 gcatcttctg gctgaagaaa gaaggattgc tgatctagaa agccagattt ctgtgcgtct
     3121 ccaaatagct aaagaacagc aagagatgat tgaagcagaa gtgaatgact tggaagagac
     3181 ctatccagca ttattacaaa atcagtcaga gatagcacca gtctcttcat ctaaaccgta
     3241 tatgctgatg gctggcggag caataatcat tgcaggtata tttgtaccaa tgcccttgaa
     3301 agtgatcctt ttactaattg gtgcaggcat gattggctat ttcttctaca aaaggcaacc
     3361 aaaaattaaa actaaaaatg atttctctga gataaaagga atgtggcaag aaaaactagg
     3421 acaacttgat ctagccttag ctcaattagc tgaagcagaa gaaaaacaaa agagaactgg
     3481 agaagaaaaa cagattcttc agcaagaagt atatcgtttt gcggatcatc ggcacttagg
     3541 aaagatggat acgctcaaac tcatgaaaga atatggacag gaagtcatag attatcagaa
     3601 attatatggc gtatttgagc ggaaacataa aagagaacag gaaatacaag aaaaactacg
     3661 acaaatcgac caaaaatttg agtttgttaa agaatggtta ccgatacaag ataagacaat
     3721 ttcagaaaaa atggtattgc tttcccgttt tatacaagag atggaacaga tgaaatttgc
     3781 cagaagctat cagcaaaata ctttattgaa gcaagaaatc aatcaattga aaaagaaaca
     3841 aaaagtatgg atacaagaat acagcgaaca attgactcaa gcaggcttat cttatccttc
     3901 ggaagtacct gtctttttac aaaaagccaa acaagccgaa cagcagatga aaagatacaa
     3961 tgaattatcg gaaatgctta ctccactatt tcctgaagag ctgacaaaag agaaattatc
     4021 tgtaagactg cgccaactga aagaaaagca atcccaactc cagcaagaag aaaaagaagc
     4081 aagtgaaaat cggcaacgac tccaacttca agtagaagag ttgcaaagag acggcacatt
     4141 ggacgaactt tatcaacaag ctagccggca aaaagcaaaa ttacaggaat tactggagga
     4201 atggggagca cttgaaatcg ccgggacatt cttaggtgat ctttcaacag agatgagtga
     4261 aagacaatta ccccagttgt taagagcagc aggtgattat ttaggtattt taacaaaagg
     4321 aaattatcag aagatattac tgaaaaatga tacactgact ttaacagacg gatatacaga
     4381 atttcccatc tatgagttat ctacaggtac gaaagatcag ctgattatgg cgatgagatt
     4441 tgcttacctc tcattagaag ctaaccgttc gatatgcccg atcatcattg atgatggctg
     4501 gcttcattat gatcatcagc gaaagtatca attagctgaa cttttagcta aatttggaga
     4561 aaagtatcaa ataatttgtt tttcttctga taaagaaatg gtaagctatt accatgagat
     4621 gaaccaaatc gtaaaacaat tagaaggagc aaataatgaa aaaaattcgt gatttagtaa
     4681 tcgatgagca gtttgaacaa tttgtcctga tcaaaagtgc agatgtaaga atcgctaaaa
     4741 acggaaaaaa atttattgct tttactttcc aagatacttc aggtactatc gatggtaaat
     4801 actgggatgc ttcggaggag gaaatcaata aatttacagc cggcacagtc gtttttctaa
     4861 acggtaaaag agaggtttat caaggaaacc ctcaagtaaa gattcttcat atgcgcttaa
     4921 caagggtaga tgagccagga gagcctagtt tatacatgga aagagcccct ttgaagaaag
     4981 aagaaatgga agaagaaatc aatcaattgt tgtttgagat caccaatgcg cattggaatc
     5041 gtattgttcg ctatctactt aataaatatc aaaaacaatt ttttgagttt ccggcagcaa
     5101 aaagaaacca tcatgcattt gccagtggat tagcttatca tactttgaca atgcttcact
     5161 tagggaaatc aatcgccgat gaatatcctg agctgaatcg agcattgttg tatgctggta
     5221 tcattttgca tgatttagga aaggtgattg aactttccgg tcctatgtcg acagaatata
     5281 cgcttgcagg aaatttgatc ggtcatctgg tattgattga cgaagaaata actaaagcct
     5341 gtcttgcgtt gaaaatcagc gaagctgacg aagatgttct cgtattacgt catatggttt
     5401 tagcacacca tggcttatta gaatatggct cacctgtacg tccaagaata atggaagcag
     5461 aaatattaca tcaaatcgac aatatcgatg cttcgatgca gatgatgttg acttcgattc
     5521 gtcaaacaga accaggaaaa tacaccgatc ggattttcgg tatggacaac cgaagtttct
     5581 atgtaccaaa agatttatag caaaaattta tttttttcat tcaaaacaaa aaaagcgaga
     5641 cataagtctt cgcttttttt gttttc
//
LOCUS       DAYYAO010000130         5339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000130 DAYYAO010000000
VERSION     DAYYAO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.136"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..414)
                     /locus_tag="ACHARI_002653"
     CDS             complement(19..414)
                     /locus_tag="ACHARI_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443651.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="ACHARI_002654"
     CDS             complement(404..616)
                     /locus_tag="ACHARI_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8443652.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="ACHARI_002655"
     CDS             1688..2290
                     /locus_tag="ACHARI_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFM8443653.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="ACHARI_002656"
     CDS             2645..2881
                     /locus_tag="ACHARI_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFM8443654.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="ACHARI_002657"
     CDS             2878..3291
                     /locus_tag="ACHARI_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8443655.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3834..4760
                     /locus_tag="ACHARI_002658"
     CDS             3834..4760
                     /locus_tag="ACHARI_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8443656.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="ACHARI_002659"
     CDS             complement(4757..>5339)
                     /locus_tag="ACHARI_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8443657.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DAYYAO010000131         5326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000131 DAYYAO010000000
VERSION     DAYYAO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.137"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACHARI_002660"
     CDS             <1..546
                     /locus_tag="ACHARI_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8443658.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="ACHARI_002661"
     CDS             complement(716..1678)
                     /locus_tag="ACHARI_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8443659.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="ACHARI_002662"
     CDS             complement(1680..3119)
                     /locus_tag="ACHARI_002662"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8443660.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="ACHARI_002663"
     CDS             3304..5250
                     /locus_tag="ACHARI_002663"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8443661.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1717 a          912 c          888 g         1809 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaagca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atggaatttt
     5281 ctttccaaaa tcaaataata tagattaatc aaatatttga gaataa
//
LOCUS       DAYYAO010000132         5032 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000132 DAYYAO010000000
VERSION     DAYYAO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.138"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHARI_002664"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHARI_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8443662.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHARI_002665"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHARI_002665"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8443663.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACHARI_002666"
     CDS             complement(2169..2906)
                     /locus_tag="ACHARI_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8443664.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACHARI_002667"
     CDS             complement(2997..3524)
                     /locus_tag="ACHARI_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443665.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACHARI_002668"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACHARI_002668"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8443666.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACHARI_002669"
     CDS             complement(4401..4886)
                     /locus_tag="ACHARI_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8443667.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1337 a         1124 c          862 g         1709 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt ac
//
LOCUS       DAYYAO010000133         5001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000133 DAYYAO010000000
VERSION     DAYYAO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.139"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..1787)
                     /locus_tag="ACHARI_002670"
     CDS             complement(21..1787)
                     /locus_tag="ACHARI_002670"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8443668.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1747..3501)
                     /locus_tag="ACHARI_002671"
     CDS             complement(1747..3501)
                     /locus_tag="ACHARI_002671"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8443669.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3611..4180)
                     /locus_tag="ACHARI_002672"
     CDS             complement(3611..4180)
                     /locus_tag="ACHARI_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8443670.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4198..>5001)
                     /locus_tag="ACHARI_002673"
     CDS             complement(4198..>5001)
                     /locus_tag="ACHARI_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8443671.1"
                     /translation="KELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVP
                     YIEKVSADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPT
                     KGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKM
                     KDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGA
                     VVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1655 a          944 c          672 g         1730 t
ORIGIN      
        1 ttaatttaaa gcctatttca tcatcgtgat tgagcttctt tttcatagac gaatttctca
       61 gatagtttag ttttctttat gagatttttg tatatttctg accttcgata caactcacta
      121 tgagtacctt gatcaactat ttttccttga tccatcacta caattttatt taccttttca
      181 atagacttca agcgatgcga tataattagt actgtcttat cttgtattaa acgatttaaa
      241 ctttcctgaa tacgtttttc attttccgca tctaaactgg cagtaatttc atctaaaatt
      301 aaaattggcg catttttcaa aaaagcccgt gctatggata ggcgttgtcg ctctcctcca
      361 gaaagtgaac ttccgttttc tccgaccaat gtatgataac cgtcaggtag tttttcaata
      421 aagtcttcgc agttagctaa acgagctgct tttttcactt cctcatctga cgcttgacta
      481 tttccaatac gtatattttc caaaatactt gtattaaaca aagttacctc ttgaaaaaca
      541 atagcgattt tgctaaacaa agaagctgga gatacacgtt taatatcata cccatcaatt
      601 aaaatacagc cttcatcata atcatacagt ctagacacta atttcaagat actagttttc
      661 ccggaaccac tagcacctac taacgcagtc acttctcctt gtttagccgt aaaagagata
      721 tgatctaaaa ttggggtatt gttatcatat gagaaagaga catctcgcaa ctcaatgtca
      781 aaagatttca gaggactatc tgatccctct tggatactgg tttccttcat agctctaatt
      841 cgctgtattt taggagctaa ataaaatatt tcaagcactg cttctttcat agaatcgaag
      901 gaatccttta tttttattgc ggctagtaag taaccaacga cgtagagtat agtcacttca
      961 cccgttaaca gtaagtgaac acctactaga atgactactg ctaaactaac atatgagaaa
     1021 atagaggata aggccatgac tgaaaaagta cttaattcta cctttaaatg aatcctttca
     1081 ctctcttcca tttttttgaa aagtctgtct tgaacttttt tagatagatt aaagctatta
     1141 atctcttgct gcaattctat agtttcttga aattcttccg agttttctct caaagtatcg
     1201 taatatttag tattggcttt cgtttgggat ttctttgata acaagattaa gacaaaacta
     1261 aataacgttg gcaaaataac agctaatccc attttgacat taccaaccaa aagcatcact
     1321 gaaataaaag ggaaaaacag agccataccg ccggctttag gtattgcatg actcatcgca
     1381 tgctcaatac cttcaacgtc agacatgata gtttgagata aatctgataa attatgtttt
     1441 gaaaaatagg ataatggtaa atttgacaaa tcgtctgcaa tttgcattct taaatgcgca
     1501 ctttctttat aagtactatt atataagttt tcatactccc tatctaacaa ccaatacatg
     1561 aaaagcaaag taacgaacga tactgtaaaa taaagccaag gcggctttac attttctaaa
     1621 actaattcgt ctatcagcat cattataatg aatgctggta ccatattaat gcagtataca
     1681 aaaaagctag aaatagatgc tttaaccaaa tcttctgcgc cctgattagt taaagcgaat
     1741 ttgtttttat aaaaactttt cattgtcaag cctccaatca tttgcattct cataattagt
     1801 gaccaattgt ttatacactt gatgtttagc cattaattca ttatgatttc cacgttctac
     1861 tattttccct tctgacaaga caagaatttc atctacagat ttaatgctgg ataatcgatg
     1921 agctatcata ataactgttt tttctttcat cagatgttga aaagcctttt gtagttcata
     1981 ctcattatct ggatcaatag atgcactggc ttcatccata ataacaatct ttgcatcttt
     2041 taaaattgct ctggcaatag ctatacgttg tttttctcct cctgacagat aaactccttt
     2101 actaccaatt aaggtatgct ctctttcggg aaatttttct aaaatcgatt cgcaaccggc
     2161 taatcttagc gcgtcaaata ctttacttgc tggcgcttga ggatttgcta tagaaacgtt
     2221 atcgtaaata ctctgattga atagtttcgt atcttgaaag acaaaagcaa ttgtgttaat
     2281 aattgtgtct tttgaatagt gatttaaagg cttaccacca attttaatct gtcccttatc
     2341 cacattgtag aaacctgaaa ttagtttagc tatcgtactc tttccgcttc ctgattttcc
     2401 tactaatgca taaattcttt tttgttctaa aactaagttg aaattttcta aaacacactc
     2461 ttctttgtat gaaaagctga cattttcaaa tgcaatgtca caatttttaa tctgtgtctc
     2521 gttcccatat tttagttggt cttgcttcat ttctaaataa agggagtcta gtgtttcgac
     2581 tgcatatctt gcttcaaaca agtgcatact tacatacatt attctcatga aagaaacaaa
     2641 aataattcca cttaaaaaaa acaacatcaa caagtcaaca gctaatatct tgggagtggt
     2701 aagagaagtt ataaaaaagg ttataggaac tatcaaaatg caaactatgc caaaaaatag
     2761 ccattgaaaa agaacaaaag gtgttttccc actcaaagaa tacttatatg caaacttagc
     2821 ataatcgtta attgctttat tcaaggcttt aaatgatgtt actttagcac caaatatctt
     2881 tataacttga attcctcgga tatattctac cgtttcagag cttaacacat ctaacgattc
     2941 ttgatatttt tgaataaaat ttgtattctc tgtcattttc tttaacaaga atacaccaat
     3001 tactaacatg gcgattagaa ccaatccaat tttaaaatta atgaaaaagc ctaaggcaac
     3061 tgataaaaca ggtgttacta tggcttgagc actatctgga atcaagtgag caattgccat
     3121 atgtgtttta gccgcgttgt catcaattgt ctttcgaata tatcccgaag aatttaaatc
     3181 aaaaaaacga aaacttgctt ctgttaaacc gctaattccc ttttttctca aattagtctc
     3241 taagcgaaac ccaaggatat gagaaaataa gccagaaaaa atataaaata aagctcctaa
     3301 agttaaccaa gcaacagttt gaaccgcata actcttagct aaatattcat ctcctacaat
     3361 aattagagac ttaagaaatt tgtaaatact gtaatatcca tagactgtta atacgacaga
     3421 aaaatcagaa aataaagtgg ctaaccatcc gtagtatttc attttaggga cataactaaa
     3481 taattttttg tatactttca ttttttcctc ccccgcattc aatttcttct cttattcgtt
     3541 tacaactgtt aatgctttcc gtaatagcgt aaattagaga tgtacaaaca gatagaaggt
     3601 aaactacgtt ctattttttg tattttttta agacttttct tccaatcaat acaccaatca
     3661 tagataaact tgctacaact aacactgaaa tgattcccca tatacctacc gcatagtgga
     3721 tcattgcatc cattgttgca gcagattgtc ctttatctat ccaatcttga cgaaaacgat
     3781 ctgtaaagaa ccatatagga atccactgtc cggcagcact gcctaattgc attagagccc
     3841 aagacgcaac ttgaggaatt gctcgatcat atccaaatac agatagaata atatcagcta
     3901 agatcgccat actaatcgaa ctgataaacc aaggtaagta cgccccgccc attaacgtaa
     3961 aaaataacaa taaaacaatt gtgtaaatag taaaaattcc ttttctaggg tagttatagc
     4021 taatataaac aaatatgatt ccagataata atccaaaaat ccctggggat atgctgtgac
     4081 caaacgcccc accaatacta ctaaccatta ttgaaaaaaa gtaaattact agggaaataa
     4141 ctgtagctaa accaataaaa atataatctt ttgttttcat atatattctc ctcaattcta
     4201 tttcgttatg tcattaaaaa tctgttctat caaagttgaa tcttctactc taagttttat
     4261 atcttttttt attccaccat tttttaaata aataattcta ttgcatgttt tataaagaaa
     4321 ttccggatca tgagtggcta taactactgc acccttttct tttaagtctt taataaggtc
     4381 agcaatcctt tccatattaa tataatccaa cccacttgta ggttcatcaa atagaaataa
     4441 attagttttt gacaatgaag ctatagaaac caataatctt tgtttttctc caccggataa
     4501 atcaaacggt aattgatctt tcattttata aagttccatt ttttttaaaa ttttttcttg
     4561 ctgatcttta ctaactattt cattccccag ggctaactcg ctcctcactg attctgtgaa
     4621 aaattggtaa tccatttctt gcattactaa aaacggcgat gacaaatttt ttttattttc
     4681 gattatcttt cctttggtag ggcgaataat cttcatgagt attcttagta aggttgtttt
     4741 ccccacaccg tttttcccta ccaaaccaat aacatctcca actttgacat caaaatttat
     4801 gtcttttaga atattttcat aagtaatttc ttcaactttt aatacatctg cacttacctt
     4861 ttcgatatag ggaacttcaa tcgaactatt aaaaatgtca aatttcctta atgcgttatt
     4921 gttttcagtt tttaacaact gaatagttgt agtttttaca gttctatttt taacaaatag
     4981 aacttgatca attaattctt t
//
LOCUS       DAYYAO010000134         4976 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000134 DAYYAO010000000
VERSION     DAYYAO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.140"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHARI_002674"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHARI_002674"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8443672.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHARI_002675"
     CDS             complement(1297..2133)
                     /locus_tag="ACHARI_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443673.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHARI_002676"
     CDS             complement(2317..3534)
                     /locus_tag="ACHARI_002676"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8443674.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHARI_002677"
     CDS             complement(3683..3844)
                     /locus_tag="ACHARI_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443675.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHARI_002678"
     tRNA            3917..4000
                     /locus_tag="ACHARI_002678"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4976)
                     /locus_tag="ACHARI_002679"
                     /pseudo
     CDS             complement(4240..4976)
                     /locus_tag="ACHARI_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1526 a         1027 c          798 g         1625 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttat
//
LOCUS       DAYYAO010000135         4950 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000135 DAYYAO010000000
VERSION     DAYYAO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.141"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACHARI_002680"
     CDS             complement(<1..434)
                     /locus_tag="ACHARI_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443676.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACHARI_002681"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACHARI_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8443677.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            complement(941..1267)
                     /locus_tag="ACHARI_002682"
     CDS             complement(941..1267)
                     /locus_tag="ACHARI_002682"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8443678.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNRIRQKSPILYRWVMNAVR
                     YEGYRW"
     gene            1400..1774
                     /locus_tag="ACHARI_002683"
     CDS             1400..1774
                     /locus_tag="ACHARI_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HFM8443679.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            1828..3714
                     /locus_tag="ACHARI_002684"
     CDS             1828..3714
                     /locus_tag="ACHARI_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443680.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQVKSRLLCKMLYNVFTIS
                     TYQN"
     gene            complement(3698..4870)
                     /locus_tag="ACHARI_002685"
     CDS             complement(3698..4870)
                     /locus_tag="ACHARI_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFM8443681.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         1659 a          780 c          720 g         1791 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttgtata gactgattgt aacatggaac
      901 aaaataaaaa ggcaatacgc cttagttgtc ggtcttcgag ttaccaacgg taaccctcat
      961 accgaacggc attcatcacc cacctataga ggatgggcga cttctgccta attctgttaa
     1021 aaataatagc tgtcaatacc agtaaattat tttttttatt ttttgaatta tcttcattta
     1081 aaatagaatt tatgttagta ctataaaatt cacaaatttt ttttagtgtc tctatatctg
     1141 gtgttgtttt gttattttcc cactttgaaa tagtttgacg tgtaacgtta aattttcttg
     1201 ccacatattc ttgagtatac ccttttttca ctcttaattc tcttagctta gatcctaaat
     1261 cctccatttt ttctcctatg taaacgatta tttacataaa attttatcat aaaaatacta
     1321 acgcgccaaa aaagttcttt attttataaa acacactaaa ataaaataat tctagtaaaa
     1381 aagataggag aaataagtta tgaaaaaatt tttatgtctt tctgctttaa tgggtgtctt
     1441 attaactagt ggaggaatag ctagtgccac agaagcttta aatctagatg tacccgaaga
     1501 ccataccgcc atttacggag gaggaatgga agctacagaa gaaggatttt cttctagtaa
     1561 attgagatat gctgctggcg gcggggattt caactgtggg gttaatggat ttaaagttta
     1621 tgcaaattac taccatgcta gagctaaaca tagtgcaaca gctaaaaatg gacgaggcgg
     1681 tcaagtacga tcagttcaga aagctggagt aagagcttat gcaacatgca atgccacact
     1741 tactggaaat actggttggt ggaatgttta ttaaaaaaga gagtttaaac tctctttttt
     1801 atttacattt gaaaggaaat tcaaaatatg ttaaaaaaaa taaacgctgt tttatcactt
     1861 tttgtctgca ttacaatact atttttttct actaatgtta taaatggtat aaagacttct
     1921 gaagcgctat ataaaaatac cgctgaaatt catatgcaat ataaagatga tatggacttt
     1981 aacagttata taaacgcttt gtatgaaata tctacacaag aaaatattaa tatttctcaa
     2041 tatagtttta ctgctgttaa ccaactaact attcttgcta caaaccctaa cgctaacaaa
     2101 cactggaaat tttctaaaaa aaatatatta gcaagaaacg attctaaagt ccattatact
     2161 aataacaaaa attctaagta ccaattaaaa cttccaaata attttttaaa tataaatatt
     2221 tatccttttt ctcatgtgga aaatattgga ttaagtgaaa tattatatgt acaaggaaac
     2281 tcaaacaaat taattcctgt actgaaaaaa tatggaacac ctactatttc caatatcaat
     2341 caatcagata cttttcaaat taatactaat attgttcttg ttatctgcta tcttagtata
     2401 tttattgtaa ttactacaat tatatttgct ttttcaaaaa tgaaagaaat cactctaaaa
     2461 aaaatgttag gctataactc ttttgatact ataaaagatt ctattaaaga gttaaacctc
     2521 ttattttgtt ctttgattat aactttcatt ttatctattt ttttgagcat atattacagt
     2581 aacgaaaatt ttttcctatc atttcttcta tgtagtattt ttcattttat aattagtttt
     2641 ttatttatca tagttactct aagtatctat catttatcta gtataaaaaa ttatctaaaa
     2701 aattcacgac cacttaagtt aaatctttgt atattaaata tctgtctatt cttaagcatg
     2761 atccttctgt taatagcttc tacaaaagta ataaataccc ataatgaagc cgaaaataat
     2821 tctcttaaat attgggaaag aactactaat ttatacaaaa caaatattac taatcaatta
     2881 aatagaaaca acaccgtaga agaaaataac tacttgaaaa aagctagtaa atttgtttat
     2941 aaaatacaga agaattataa aacttttatt atcgcgccat acaattacgc aactatccaa
     3001 gagaataata aagaattttt tattggacaa aaagtttatc caaattttga agactatgtt
     3061 agtcaacctg ctggtgcagg aatatgtgtt gatttaaact atctaaaata ttacaatcct
     3121 atttcttttg aagaatctac tgacttaaat ttaataaact ctgatccact aactacttac
     3181 attctagtac ctaaaaaata taaagaatat gcaaaaatga ttgaaaaaaa ttatttagaa
     3241 gacatacagt ttagacttga ggactctcct cctcaagctc cttataaaaa tcctaatttg
     3301 aaaaatttaa aaataaaact aatatttgtt aataataacc aaaagtattt tagttttaat
     3361 accttatatg gaaaggcaaa agataactat acaataactg atcccatagt tagagttatt
     3421 aatccagaaa taacagaatc attattttgg ggaaatatat tgactagtga tggtggatta
     3481 ttttttgatc agaaacatac ttcaaaccaa aactttttta atcaaattaa tccgtatata
     3541 aaagaattta acctagaaaa tatcataaat ttttcttatt ctgtttttca agaaactggt
     3601 gaattattat ctcaaaaaaa agtaaataaa ttaaatgcat atctacaagt caagtccaga
     3661 ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt
     3721 ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat
     3781 tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat
     3841 tcagtcgttc aattagattg gtactcttta gtcgattgtg ggaatttcct tgtacggtat
     3901 attgaaaggc gtcttcgaat ccatcatcca atgatgcgca agcttttgaa tattttggtt
     3961 gatcgatata atcatgaatc aatcgatttt tagcctcacg cgctaagtta atatctgtga
     4021 acttaaaaat tcctttaaca gcttctctga aagattttga atttttttta ggaatggtgg
     4081 taaagatatt tcttaggaag tgaacttggc atctttgcca acttacgttg gtgaaggatt
     4141 ttctaatggc agagactaat cctttgtgcg catcagaaat aacgagttcc gtaccttgta
     4201 aaccgcgttc ttttaggtat tcaaaaaatg ttgtccaggt ctcttcgctt tcgccacttt
     4261 gaatcatgaa gccgataatt tcacggtcgc catctttggt tattccaatc gctatatgac
     4321 agctttttga gagtactcga ttttcttctc gtacttttat atagagtaca tcggtcatta
     4381 agtaaggata atttttttct gataataaac gattctgcca ctcgttaacc ataggttcta
     4441 gctgttctgt taagctagaa acgaaggact tagagacgga tttaccacaa agttcttcca
     4501 caatttttga tactttacga gttgaaacgc ctgatacata catttccaac attgaagcca
     4561 tgagggcttt ttcgtttcgt tgataacgtt caaacactgt gggtgaaaaa tggccatcac
     4621 gtgttctggg tacttttaat tctagcgtgc ctacacgtgt cgtaaagctg cgctcataat
     4681 agccatttcg ttgactttgt cggttttctg ttcgttcata ttcttttgct tgaatatatt
     4741 ctgttcgttg attttccatt agttgattaa ataccgttgt taaaatattt ttagaaacgt
     4801 catcctttac agaatattca ataatgcttt gaatctcttc gcttttcagt gtaaaatgta
     4861 cttgggtcat gtaaaagtcc tcctgggtat gtttttgtcg ttaaaaacat tgtaccgtaa
     4921 aaggactgtt atatggcctt tttactttta
//
LOCUS       DAYYAO010000136         4930 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000136 DAYYAO010000000
VERSION     DAYYAO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.142"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..970
                     /locus_tag="ACHARI_002686"
     CDS             83..970
                     /locus_tag="ACHARI_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8443682.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="ACHARI_002687"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="ACHARI_002687"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8443683.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="ACHARI_002688"
     CDS             2272..2718
                     /locus_tag="ACHARI_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8443684.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="ACHARI_002689"
     CDS             2991..4343
                     /locus_tag="ACHARI_002689"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8443685.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="ACHARI_002690"
     CDS             4514..4801
                     /locus_tag="ACHARI_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8443686.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1394 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt
//
LOCUS       DAYYAO010000137         4914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000137 DAYYAO010000000
VERSION     DAYYAO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.143"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..1061)
                     /locus_tag="ACHARI_002691"
     CDS             complement(21..1061)
                     /locus_tag="ACHARI_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8443687.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            2227..2313
                     /locus_tag="ACHARI_002692"
                     /pseudo
     CDS             2227..2313
                     /locus_tag="ACHARI_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2867..3091
                     /locus_tag="ACHARI_002693"
                     /pseudo
     CDS             2867..3091
                     /locus_tag="ACHARI_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3351..3605
                     /locus_tag="ACHARI_002694"
     CDS             3351..3605
                     /locus_tag="ACHARI_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8443688.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            3606..3923
                     /locus_tag="ACHARI_002695"
     CDS             3606..3923
                     /locus_tag="ACHARI_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8443689.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            4482..>4914
                     /locus_tag="ACHARI_002696"
     CDS             4482..>4914
                     /locus_tag="ACHARI_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443690.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT         1716 a          701 c          767 g         1730 t
ORIGIN      
        1 atcaatttgt cagtgaaatt tcactctaac cagtctttcg taatagtttt tgggaagctt
       61 tcagaattca aacgtttttc acttgatact ttagcagcta ttggtttttc gataaaccaa
      121 ttttttacat agacaaaaat caggttctgg atattttcaa ctttttcggt ttttaatttt
      181 tgataggctt ttaacagtgt cgtgtataat tcttgttctg cattgacata ataactatcg
      241 agttcttcaa aaattattga aataccactt tctgtttcgg cttttttctt tgcattatga
      301 atagtctgga cagttttttg agcttcactg taacttgaag aaaatgtttt tattaaattt
      361 aatactcgtt cagggataaa cgtagcaatg ctgtcgtttg tcataattgt ctcaaaattg
      421 tctaacaaca attgatcttg taggcggtct ttttcagtgt ctatattgtg tctattagtg
      481 tctaatatat tattattaag ttctggtatg attttcatac cctctgattt atcaacgttt
      541 tggcacggta tgattttcat accctcttga gccccaaggg attcagcgtc tgatttttcg
      601 tcgagggtgg gtatgatttt cataccctct gatttatcaa cgttttccct attctctcta
      661 tcaaattttt cgagtaagta gatatcattt tctgagactt ttaattctgc tagatatagt
      721 ctattaggat tatttttccc taactgtttg ttaaatccca tctgcttttg taataataaa
      781 ccaaaggatt ccaactcttt tttgattgct atcgcttttt gtttttggca gtttaatacg
      841 ctacatagtt catttgtcgt ataagtaaaa taaacattac cttcttcatc gatttgccgt
      901 ttgctgattg cgatctctaa tcttgctttt aaaagcatat aggcaatctt agttgaatct
      961 ttcatttttt tgtattcgtc agaaacaata aagacttttg gaaattgata aaataattcg
     1021 ttgtaaactc tatccgcctt aaaaaaatta aaatcagtca ttgtagatcc tctactttca
     1081 cgttgtttta tttatgtaaa attacctact cttttcgaaa atagcaaaga taaataaaaa
     1141 atgcaaacgc attaaaactg ttttttaatt aaatactacc taaaatcaga aataaaatga
     1201 attatgtact acctttaaag tcattaagaa ctactttaaa taaatattat ttataattaa
     1261 atactaccag aagaacgtat gaataaaatt tagttttaaa attaaagata attaagtact
     1321 accagtgtca aaacaaaaaa taaattaaga actaccttat gcatataaaa catactacca
     1381 ttactagtaa tatatactac cttatttcag tagtatatat tatattcttc ttataagaca
     1441 aggctttagg ctagaaaaca atagattata tactactaaa aaatacaata agtactacca
     1501 atgtcagata tatataataa ttatgtacta ccattaaaat taacttattt aataagatac
     1561 tactgtgtta atctgaaaaa aattacttta aagtagttac atactacctg ttaaattaga
     1621 tactaccatt aatacttaaa ttagatacta cctatattta tattaagtac taccgttcta
     1681 ggaataagat aataaaaaaa aactaccaaa ttgcaattaa atactactta aaatgtaaaa
     1741 aataagaact acccaaatta ggtagttctt aataagagaa tatcagataa atttgtacaa
     1801 gtcataaaaa aattaatctt aaatttttaa atatatatac ctgttaatta taaactactt
     1861 tatttaggta gtacataatt aacagataaa actacatgct tgtagtttgt ctagctaatg
     1921 agttgcatat gatttatata aaggtgtgca gcataactta gttgaagtta agagaataga
     1981 ataaatattt tttgttttgc taataattaa acactaccta aatatggtag tgtttaatta
     2041 tataatattc attttgttac aaataatcgt attataaaag cataacaatt gggataacta
     2101 ttttttagat tatttaagta ctacttaaat taggtagtac ttaaattttg gtttaaggta
     2161 gtaggtaatt aagaaataat atatatcttc ttttataata gaaggggaga ggcctagtta
     2221 aaaacagcga caattaataa caatgtattt aatgcttcac aaaatccatt tggtggacaa
     2281 tgcattgtta ttagcgatga tctaccgttc taagtagttt tttctattgg ttgcattcgt
     2341 gactaattat ggtataatta tgagtaacaa agaggtattt cattatatca tcaaacccca
     2401 tcagcgtgta ggctgatggg gtttttttta cgtttctata ttatagaatt aactggtagg
     2461 cttactgtct tttttggcaa ttttgcaatc aagccacttg cagatgtagt gtgcagttac
     2521 ctctgcaaat ggcttgattg caaaaacaaa atcaaaaagg tatttcatta tatctcctcc
     2581 tccccttgcc agattaggcc ggggtagcag acagcaaaac tattataaca taactgaaat
     2641 acctaaatga catatacaga tatgataatt tatgtattga tttttctaac aaaagcacat
     2701 attttaatat atactaccta ttttaggtag tatatatttt tagatacaat atcgatcagt
     2761 tttgaggaaa tgaaattcta gatatttttt gctacataga gttactttca atatttttta
     2821 acgtaattag gcagaagtca ctcatcctct ataggtttgt gatgaaattg ctattgatcc
     2881 atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat tggtctaatg gatttaagta
     2941 tgcggtcacc gtgtatagag ttgcttatga gattcaagaa aataaagtta ttccagtatt
     3001 gctatatgga acacataaca atttttatga attagccgag aacactcgca actttattcg
     3061 tgtagatgaa tcggcttcgg ttgagttata actttagcta tatcaatcaa ccgaccgaca
     3121 tatgttaaat ctatatataa taaaaatacc tacttaaatg gatgttaacg gaagtgtatg
     3181 gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt ctaagtggct
     3241 tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg gcaattgtcg
     3301 ttataacatg ttataattta tttatcaaaa actaaaagaa gtgtgataac atgggaaaag
     3361 aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc tctcttggat
     3421 taaaaagcgg agacactatc caagccaaac aagaaggcaa cttattcata ttagatacta
     3481 ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa gactttgaga
     3541 aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac ggctggagtg
     3601 aatagatgaa agagtacacg ctagtttatt ctaaaagttt ccagaccagc cttgaaacga
     3661 taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa agatttgtag
     3721 gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat gaggaagttt
     3781 ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa agctatgcct
     3841 tattttatcg gatcgaccat gaaaaaaaag agatacttgt aggacgtatt ttccaatcta
     3901 aacaaatgaa actaggcttt taacaataat taggcaaaag tcgctcatcc tctacaggtg
     3961 ggtgattaat gccgctcgat atggttgtta accgtcggtt actcgaagat tgatatataa
     4021 gacttagtac ctttttattt tgctccatgt tatggccgaa ggtctaaaga tacaatcatt
     4081 agtctagaga aaaattaaga accgtaggaa ctacggggat cgcttggtga ataagagaca
     4141 ctgctattag tgaaaaaata agatatcaag tacgctctat tcccaaaaat ttcgtgactt
     4201 tactcatgag aggttcaagt agtatatttt taatcaaaat tgacgtaact tttaataaat
     4261 actacctaaa taaggtagta tttatttttg tgttttaaaa gaggaagtcc aatatatcta
     4321 tgggaaactt cttaaatatt atgttggtac atgaaaatat acatccctgc tagactattt
     4381 acaacatttt actttgtacg tattcttgtt ttaaaaggaa tgggataaaa tataggtaca
     4441 ataactattt gatgctaaat ataattaatg gaggagagat agtgaacata cttaatgaaa
     4501 aaagatatgt atggtttgct ggtaaaaaat atattactac tttcaaaaga ttatccgagt
     4561 taatgcagca gactgttaaa agtgctatgc ctgaaggata caagtttaat tttgttcaat
     4621 cttcagtatc tactgaaagt atctattttt caattctttc tgagagtagg ctttttcatt
     4681 ttagaataag ttctcatgta aagaaaactg atgttgatat atatacgttc cgtacaaata
     4741 attataaaaa tttaaatgat ttgagtagaa gtatcagtga atatctcaag gggaatagct
     4801 atctcaagat tcgattttca catatattaa ttttactacc atatttgaat gagaatttca
     4861 ccctaattca agtagataag tctttcattg cctcaggtaa tcaaggaaat atga
//
LOCUS       DAYYAO010000138         4833 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000138 DAYYAO010000000
VERSION     DAYYAO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.144"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..2410
                     /locus_tag="ACHARI_002697"
     CDS             8..2410
                     /locus_tag="ACHARI_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8443691.1"
                     /translation="MLSFSIILSYLIVDLKLCNRTVLSTQKWLNKKYSNVTGFDKVPE
                     NGRTGWPTIYGLIEGLQVELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQ
                     GAFWCKGINPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFV
                     LVPGGDSKIRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATG
                     SYGNGTTSKTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKF
                     MVLPEYTEIADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYL
                     TGTVGIGKDERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAID
                     AAKNLNIPLTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCS
                     QIINNGYASFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSG
                     RDSGFNELSNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYS
                     GLSWDIISSPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQM
                     FLADTINDSVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQD
                     VDAVNVYTLAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLE
                     NDETVDILGVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSK
                     LN"
     gene            2490..2885
                     /locus_tag="ACHARI_002698"
     CDS             2490..2885
                     /locus_tag="ACHARI_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443692.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3210..3827
                     /locus_tag="ACHARI_002699"
     CDS             3210..3827
                     /locus_tag="ACHARI_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8443693.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3868..4167
                     /locus_tag="ACHARI_002700"
     CDS             3868..4167
                     /locus_tag="ACHARI_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM8443694.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4201..4368
                     /locus_tag="ACHARI_002701"
     CDS             4201..4368
                     /locus_tag="ACHARI_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8443695.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4413..>4833)
                     /locus_tag="ACHARI_002702"
     CDS             complement(4413..>4833)
                     /locus_tag="ACHARI_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8443696.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1647 a          675 c          956 g         1555 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gttttaagta ctcagaaatg gctaaacaag aaatacagta atgttacagg
      121 gtttgataag gttccagaaa atgggcgaac agggtggcct acaatctatg gacttattga
      181 aggactacaa gtagaacttg gtattactaa tctagtagct aattttggac ctaccactga
      241 aaaaatgtac gataatcagg ttactcctaa atggggcaaa aatctaccta aaaatattgt
      301 atttcttatt caaggtgcct tttggtgtaa ggggattaat ccaggtggct tcgatggggt
      361 atatactcca tatttagata ctgctgtaaa agagttgcag actgatgcag ggtttaatgg
      421 aagcgctgta actggagttt tggatgcgaa atgggccaaa gcgttatttg atatgagtgc
      481 ttttgttctt gttcccggcg gagatagtaa aattagaaaa atgcagcaat ggttaaatgt
      541 aaattattta gaatacacgg gaataatgcc ttgtgatggt atttatcaaa gaaattcaaa
      601 tcaagcatta atttttgcat tacaagctga actaggatat tctcccagtg aagcaacagg
      661 gtcttatggt aatgggacaa ctagtaaaac ttatcctgtg tctgaaggta actcgggaaa
      721 ctatgtaaga ataattcaat atggattata tgttaatggt tattttgaag acggtacatt
      781 cgatggtatt ttcactaaat atatgggttt agaagtactg gcattccgaa aatttatggt
      841 attgcctgaa tatactgaaa tagcagatgt tgttgtaata aaaggactat tgtctagcgc
      901 tggagatatt agaagatctg cagatggcgc agatacttct acgcaattaa ctagatctca
      961 aatccaaaca ttggttgata atgatataaa aatagttggt agatatctta ctggtactgt
     1021 tggaattgga aaagatgaac gaaataagta tctaactaca gaggaattga ataatatttt
     1081 ttctaaaaat ttatctgtgt ttcctatcta tcaagatgga ggtgcagcat tagcttattt
     1141 tacttatgat cgtggcttat cagatggtaa gaaggctata gatgcagcaa aaaatctaaa
     1201 tataccatta acaacagtta tttattttgc tgttgatcta gacatgttag gggaagaaat
     1261 tcttgcatat gctggagaat attttaaggg ggtatctgct gtaatgtcat atagcgggta
     1321 tcaaacgggt gtttacggaa caagaaatgt ttgttctcaa ataatcaata acgggtatgc
     1381 ttcgtttgca tttgtttcag atatgtcgac tggttatagt gggaatttag gttttcctat
     1441 gcctagacaa tggtcttttg atcaatttgt tgaatttacc ataggaagtg gcaacggagc
     1501 tgttggtatc gatttaatcg ctacatcggg tagagatagc ggatttaatg agttatcaaa
     1561 tgatactaat aatgataatt acattgccaa atacaatcag aaggttataa atcagatggt
     1621 acgtgcgtat caatatctat cacaagctgg tcttgataat ccttggaacc ctcatttgac
     1681 attttataga tatgtaaatt attctggttt gtcatgggac atcatttctt ctcctgtaac
     1741 agagcatgat agaaaaattt atgatgaata taggaataaa ttaactaata gcgagggcct
     1801 gtataattat tttatagatc ctaatactgg ttcaataata ggtttgcctc acttaattgt
     1861 gacgttacaa tcacagatgt ttctagccga cacaatcaat gatagtgttt ctgactttgc
     1921 aggttggtta ggagatttaa tgacttgctg gggtgaagtt aagaaattag gaatacaaat
     1981 ggagcaaggt gtatttgcat tagtaggtac ggctgcaggt ggaacattta gtctagaaga
     2041 tttataccaa gatgttgacg cggtaaatgt ttatacctta gcaaaaagaa attctgattc
     2101 aacagcttta tcggctttaa aaacgtacta ttgtgataag ggtgctattg atagatttaa
     2161 tcaatttgtt aagaacagat ttggttcttc agataaaatc gcttcagtaa caaatagttt
     2221 attagaaaat gacgaaacag ttgatatctt aggtgtaggg ataatttcat ttttcattga
     2281 taaaatagtt aataattcac ttgggacaga ttttacttat cttgatttta ttggagatga
     2341 ggggaaagtt gcaaatggat tttctgaaaa aattattcaa tttcaagaaa atagaagtaa
     2401 acttaattaa gaatcgttta ctatttaaac agtgtccttt tcctttttaa atatatacga
     2461 taaaataagt atatgaattg taaaaggaca tgatgattat ggaagaaata gaatggaata
     2521 atctgactca aaataataaa aataattctg gatgtggaat tatctttatg tttgttatag
     2581 ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt gcgaatactc
     2641 ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct tatgatccaa
     2701 gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg tggtttgaag
     2761 ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat attgtaaagg
     2821 caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt gttgtaatag
     2881 aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa
     2941 aatataccat ggaagacata aaagaaaaac tggataacta gtttatttaa tggcagaccg
     3001 ttaaggcctg ccatttttat gtattttagc ggaataaata taatcatgcg ggagtgtaaa
     3061 cttactgttt caaaaagtat actttttgac atgccttgat tttctttgtt tatgtatggc
     3121 ataataacca cataatacaa tttgaaacgt ttctgattta atctgattgt tttgaaacaa
     3181 ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt tttttatatc cgggtcagta
     3241 gttatgatca atcgatcgaa cgacaactga tttccgcgaa ggaattagga atccctgaag
     3301 aatatatatt tgtagaaaaa gccagcggca aagattttaa acgccctgaa tatcaattaa
     3361 tgaaacggat gtttcgaaaa ggagatttac tctatctaca atcattagat agactaggca
     3421 gaaataaaca aatgatttta gaagaatggc gagaattaac taaggaaaag cagattgata
     3481 tttctgtgtt agacatgcct ctgttagata ctgctagtta caagcaacta gatgggttag
     3541 agacgcttat ctctgactta attctgcaat tactcagcta tatggccgaa gacgagcgga
     3601 aacgaattaa tagccggcaa agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat
     3661 tcggcagaaa gaaaattgct ttacctcctg aatttcctgc gatctattct gactggaaag
     3721 caggaaagat aactgcagtc tatgctatgg atcaattggg catgaaacga aatacctttt
     3781 atcgacgggt gaaagaatat gaacatacct tgcaccactt ttcttgatag atataacagc
     3841 tattagacgg gaagggagag tttgaaaatg gacttaacga atcaaagggt cgtttttact
     3901 ggaacgctgc agcaaatgac acgaacccaa gccatcggtt tggttcactc tttaaacggc
     3961 cattgtcaat caagtgttac ttcaaaaaca aatttcctgg tttgtggtca gtattctaag
     4021 tcgttgtttg atgattctct ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg
     4081 ttaggtcatg agattaccat tttatcagaa gtcgaatttt atgcgttaat tagtcaacag
     4141 ttgaaagaat ggcaacggta tttataatcg ttagtaaaat ttacaaggag gaaaattttt
     4201 atgaagcgaa aatcttatga tcgttggggc attgaaataa ctagttttaa tcgaaatatt
     4261 gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac
     4321 ttttactgtc ctaaatgcca tcgccgatac ggggtacatg gaacatagaa acgaaatagt
     4381 gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat cattcgcttt ctttatttta
     4441 taaatagttg gtcgagagat cccgttttct tttgcaatct ctgcaactgg aatgccttgc
     4501 tttaacatgt ccaccacggt tttaaaaact aagcgttttt gcggatctgt tgagttaata
     4561 gaatactcga ttggtcgtcc cttatatcgg ccggcttttt gagccaataa tactccttgc
     4621 cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa tataacttag cagtgataaa
     4681 aacatatcga acatagcggt atccaggagt tcatttccag tattaaaatc taaaaactgt
     4741 gcatctaata tttttaacgc tacttttttc tgtttcataa aagcaactgt attttttata
     4801 tcttcataat ctcttcctaa acgatctagt gat
//
LOCUS       DAYYAO010000139         4802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000139 DAYYAO010000000
VERSION     DAYYAO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.145"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACHARI_002703"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACHARI_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8443697.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="ACHARI_002704"
     CDS             complement(2065..3063)
                     /locus_tag="ACHARI_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8443698.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="ACHARI_002705"
     CDS             complement(3289..3942)
                     /locus_tag="ACHARI_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8443699.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACHARI_002706"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACHARI_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8443700.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1412 a         1007 c          744 g         1639 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aa
//
LOCUS       DAYYAO010000140         4741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000140 DAYYAO010000000
VERSION     DAYYAO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.146"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..1023)
                     /locus_tag="ACHARI_002707"
     CDS             complement(25..1023)
                     /locus_tag="ACHARI_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8443701.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1098..1394)
                     /locus_tag="ACHARI_002708"
     CDS             complement(1098..1394)
                     /locus_tag="ACHARI_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443702.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1387..1680)
                     /locus_tag="ACHARI_002709"
     CDS             complement(1387..1680)
                     /locus_tag="ACHARI_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8443703.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1826..2377)
                     /locus_tag="ACHARI_002710"
     CDS             complement(1826..2377)
                     /locus_tag="ACHARI_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8443704.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2551..2850)
                     /locus_tag="ACHARI_002711"
     CDS             complement(2551..2850)
                     /locus_tag="ACHARI_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8443705.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2877..3152)
                     /locus_tag="ACHARI_002712"
                     /pseudo
     CDS             complement(2877..3152)
                     /locus_tag="ACHARI_002712"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            3490..4173
                     /locus_tag="ACHARI_002713"
     CDS             3490..4173
                     /locus_tag="ACHARI_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8443706.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4210..4707)
                     /locus_tag="ACHARI_002714"
     CDS             complement(4210..4707)
                     /locus_tag="ACHARI_002714"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8443707.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1404 a          907 c          694 g         1736 t
ORIGIN      
        1 ttctttgata aataatattt tcaattattt aatcatttga aaattgttag tcattccaat
       61 acgttgagaa attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt
      121 gtttccatca tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat
      181 tagatctttc atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg
      241 agaatcatac aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact
      301 tgaaattcct gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc
      361 ttctactact ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa
      421 ctgttgattt tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg
      481 cgttaaataa actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa
      541 atattctcca gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt
      601 tccatcttct gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc
      661 attaatcacg gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta
      721 gcttttatat tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga
      781 agctttattc gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga
      841 acttgcactt gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt
      901 tttacttttt ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt
      961 ctcagatgta caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt
     1021 cattatattt ccccctttca atttctccct cacgtactta ttctatcaga caattaattg
     1081 atctaaatgg taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag
     1141 cggtctcttc cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat
     1201 cctctacagc gataagttct cattattttt gggctattag tattattttt cttagttaga
     1261 gctatgagtc caactatgat taccccaact gtcttaatta cttttccttt atctatattc
     1321 ggatgcgcaa gcatatattg atataaccaa tatccaccgt aaattaaacc acctaataca
     1381 atcagcttag ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct
     1441 tgcagagaaa ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat
     1501 catgttttcg attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat
     1561 ctgttgccct cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc
     1621 ctcaaaaaag tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat
     1681 ctacacaccc cctgttattt tttacataat gaaaatagca tattactggg tacattgata
     1741 attcatataa catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt
     1801 ataagtaaca tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca
     1861 attgccattc attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta
     1921 tcaatacttt ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt
     1981 gttcataagc cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga
     2041 ttttcttttc tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa
     2101 gaaatacccg aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag
     2161 taaatcctaa ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg
     2221 gttctgtatc tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc
     2281 ctttgatcgc ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa
     2341 cttctgtttg tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt
     2401 ttgttcaagc acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt
     2461 catattcttt ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa
     2521 catttcgcaa tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca
     2581 aaccaatcgt ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt
     2641 aatgaaataa actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt
     2701 catcactaaa cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt
     2761 ttccatcgta acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt
     2821 ttccagtact tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag
     2881 tattcttctg gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga
     2941 gacactgatt tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct
     3001 tcttgacgat gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga
     3061 tagtctaaag gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt
     3121 aattccaccg gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga
     3181 aacatttctt ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg
     3241 gcttcatgga tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact
     3301 acacaattat taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc
     3361 gtggtgctag ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa
     3421 aaaacgcata tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat
     3481 ggttacgtcg tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat
     3541 atccccgatt atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat
     3601 cgtgttttaa aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac
     3661 tatttagccg ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt
     3721 gattatttag atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt
     3781 gcacaacttt cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat
     3841 ggttttcggg tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta
     3901 ccgattctag atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct
     3961 tacttgtatg acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt
     4021 ggtaccttgt ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc
     4081 gatgatcttt ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa
     4141 ggaaagcgaa ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag
     4201 gacgtttttt tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg
     4261 aaaatgtttg atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg
     4321 atttttttca taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt
     4381 cgcttcatcg agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag
     4441 ccgttgaatc tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg
     4501 atgatcctta ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc
     4561 agttgtcaat tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa
     4621 cgattcataa aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa
     4681 atacgctagt gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa
     4741 t
//
LOCUS       DAYYAO010000141         4719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000141 DAYYAO010000000
VERSION     DAYYAO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.147"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1156)
                     /gene="msrA"
                     /locus_tag="ACHARI_002715"
     CDS             complement(365..1156)
                     /gene="msrA"
                     /locus_tag="ACHARI_002715"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8443708.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1347..1982)
                     /locus_tag="ACHARI_002716"
     CDS             complement(1347..1982)
                     /locus_tag="ACHARI_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8443709.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2248..3300)
                     /locus_tag="ACHARI_002717"
     CDS             complement(2248..3300)
                     /locus_tag="ACHARI_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8443710.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3313..4251)
                     /locus_tag="ACHARI_002718"
     CDS             complement(3313..4251)
                     /locus_tag="ACHARI_002718"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8443711.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1535 a          990 c          725 g         1469 t
ORIGIN      
        1 tagttattgt ttgcgctttt atacggaatt tagttcatca ttaatgtgga attcgattag
       61 gtgtctatta attatcaaga tgattctcta caaaattcca tagaaaaatc aagattctac
      121 aattcgaatt ctctttcaca tggaaataaa ggctagtaaa aaaacaggag ttgtgacaaa
      181 agacttgtca tacctcctgt ttccttgcga cttgaagctg aatgctttgc gccacaacct
      241 tttcctcggt gttctcaaag caactcctcg aaataattca taactgtcca aaaatatgaa
      301 aagctatttt tgtcagttgc tcattatttt gtcggagctg aacgctttgc gccacaacct
      361 tcctctatct atgtcgctga aaaaataaat gaagctgatg cgaatgcttg atctttttat
      421 atcttttagg ttgcttctga taaaattgtt gatgatgatt ttcagctggc caaaaagtct
      481 ttgcttggcg aatctctgta acgataggac ggctaaatcg gttagattga atcagttgtt
      541 ctcttgattt ttcagccaat tgcctttgct tttcattaac aacaaataga attgaacgat
      601 attgtttccc ccgatcttga aattgaccaa aggcatctgt agggtctgaa atttgccaat
      661 aaatctccaa cagctcttca taggttattt gacgtgtatc aaatatgatc tcaacagctt
      721 caacatgccc agtatatcca ccactgactt gatcgtaatt tggttttttg gtctctccac
      781 ctgtataacc agataacact gagattacac ctggttttgt ttcaaaaggc tcaaccatac
      841 accagaaaca gccaccggca aaaatcgccc gttcttggta taagggtgaa gttatcttat
      901 gtttggcata atcataagcc tttgcactcg ctgactcccc tgtgattctt aaataaaaat
      961 ccatcgcatc tggtgtcaaa ttatctcgta gtgctagcgg acgcaagttg acctctagtt
     1021 tttctatcac ctgctttatc gacatttttg ctaggtcttc ttttgcctct tcaaattgtg
     1081 ttctttccca ttctcttgta tttcgattta aaataatatt ataaaggtct atttccatct
     1141 gctccacatc taacatgaag tcaccctctt tcaagttttc ttcttattca ttcaaaaaat
     1201 gactacctga aatttagcac aacgcataac aagttaagac taacttgctt ttcgctctcc
     1261 tcatctctat tcttagtcga acacatccac acgaatggtt acccatagag aaattctttt
     1321 acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc
     1381 atcggcaaat agttaaagaa aaaacttaag cgttgaaaga agcccaattg cttaaagaag
     1441 gcaaactgac taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta
     1501 ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc
     1561 agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta
     1621 tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa taacccagta
     1681 aaaatacaat cacctatccc atataaacca actgttgcaa cagcaagtat tgcaagtgtt
     1741 tttgatgttc caataaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca
     1801 gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca
     1861 ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc
     1921 gataaatcac taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc
     1981 attttcttct cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca
     2041 ttttaacaca tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt
     2101 tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc
     2161 tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat
     2221 tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc
     2281 atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat
     2341 gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg
     2401 accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag
     2461 agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt
     2521 tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc
     2581 tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat
     2641 cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga
     2701 aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg
     2761 ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa
     2821 agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac
     2881 aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc
     2941 tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc
     3001 caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat
     3061 cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt
     3121 tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa
     3181 atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc
     3241 tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat
     3301 tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa
     3361 gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt
     3421 gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata
     3481 atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac
     3541 atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac
     3601 cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca
     3661 tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc
     3721 agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc
     3781 ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg
     3841 accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg
     3901 tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata agacctctgt
     3961 atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt
     4021 aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg
     4081 tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgct ctttgatttg
     4141 ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat
     4201 taacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc
     4261 ctcctttttc tttctattat attcatttga gaatcatttc acaaccttgt tttgttattc
     4321 ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa
     4381 aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga
     4441 aacattcata gaaaaaacct cactcctctg attataaaag agggggcatt aacctcgtgt
     4501 atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg
     4561 tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca
     4621 atacacttat ttaaaaactt ctttcccaga ttttcttcca tttttacagt tataactagt
     4681 attatcatga aaaatagcct tttattgtct atgggaata
//
LOCUS       DAYYAO010000142         4635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000142 DAYYAO010000000
VERSION     DAYYAO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.148"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..519
                     /locus_tag="ACHARI_002719"
     CDS             175..519
                     /locus_tag="ACHARI_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8443712.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACHARI_002720"
     CDS             520..939
                     /locus_tag="ACHARI_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8443713.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ACHARI_002721"
     CDS             complement(1031..1381)
                     /locus_tag="ACHARI_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443714.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2678)
                     /locus_tag="ACHARI_002722"
     CDS             complement(1347..2678)
                     /locus_tag="ACHARI_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8443715.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2934..3500
                     /locus_tag="ACHARI_002723"
     CDS             2934..3500
                     /locus_tag="ACHARI_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8443716.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3645..3732
                     /locus_tag="ACHARI_002724"
     tRNA            3645..3732
                     /locus_tag="ACHARI_002724"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3677..3679,aa:Ser,seq:cga)
     gene            4066..>4635
                     /locus_tag="ACHARI_002725"
     CDS             4066..>4635
                     /locus_tag="ACHARI_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8443717.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaaacac ctgtaaaacc aagataaaaa tactttcttt tctgtgaaac gattcgtttt
     2641 tgaggatgaa tataaataac gtcagtttcg ttttccatta cacaccttct ttttcatttt
     2701 ctttaactat aacttgaaat gaaagatttg gacaactatc atttaggtca aaactaaaaa
     2761 tgtaagggaa cccattgaat ttaaccaaat ttttttaatt ttgcaaaaaa aagttaaaga
     2821 agactgaaaa aaaatttaaa atataacgtt ttgagtaaaa tgtttgcaaa aaatgctaaa
     2881 aatagggtac aataaaacaa catatactgc agtaaagaag tgggggagtt ttaatgagaa
     2941 gaaaagtcta taccaaggat caaattctaa aagctgctta tgatgttgtt gcaaaggaag
     3001 ggtttaaagg atttactgcg agaaatattg ctaaaaagat ggggatttcg acacagccga
     3061 tttatctgga attcaaaaac atggaagatt taaaaaatac gttgctggat tctatctttg
     3121 aacaactaaa agaagatgtt ttccctattg aacgtactgg tgataaaatc attgatctcg
     3181 gtttgaacta tatctacttt gcaaaaaagc atcatagtct ttacatggca ttgtacattg
     3241 atgaacatgg ggacggaaag aaaatgcatg atttttcata caattatttc cgtgaaatag
     3301 ttaaagaaga tgaaagatac aatcaattat cggatgaata tgtcgatgct ctgcatactg
     3361 gtacatgggt agcagtgact ggcttagcgt cattgatgtc atcaaacatc atgcatccaa
     3421 cagaagaaca aatcattgca ctgatcaaac ggacaattga ctcgatttta gcattagata
     3481 atcctgaaat aatcagatag tgttctatca gtatgaattt gatcggtaag tccgttggct
     3541 gcaaactgtc aatggacttt tttgctaaaa aaagcgattt ctgttattta tctattagaa
     3601 ataggcttga catttcgtga aaaaagttta aaaatagtac ttacggaagg ttggcagagt
     3661 ggtaatgcac cggactcgaa atccggcgaa ccggctaata ccggcgcgca ggttcaaatc
     3721 ctgtaccttc cttatatcaa cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat
     3781 aagaacggcc aaacaaaaat agcttttcct attttttcgt ttgattgggc ttattaccgc
     3841 agatgcaagc ttttgaacac cgtttgttcg gaataagggg ctgtgacaag acttttgtca
     3901 cagcccctta ttgttgtact tttaacctac aatatttttt gattccccct gagtactatt
     3961 ttttataaat gctagtaaat caatcttctt tggccttttt ttgataaaaa aaatactaga
     4021 tgtaagataa aagtgtaaaa acgatttgtt ttaggagggg aaacgatgtg taccgcaatc
     4081 acttatgcaa cgaaagacca ttattttgga aggaattttg attatgagat gtcttataat
     4141 gaagtggtca ctattactcc aagaaattat cgtttcgatt ttagaaaggt aaaaaattta
     4201 gataaacatt atgcaatgat aggaatagca gctggggtat ccaactatcc actctattat
     4261 gaggctacaa atgaaaaagg gttgagtatg gcaggactga attttcctgg caatgctgat
     4321 tataaagaat tacaagaagg aaaagataac gttgcaccat ttgagtttat tccatggata
     4381 ctagggcaat gctcaaccat agacgaagct aaagaattat tggctactat caatctagtc
     4441 aatatcgatt ttagtgaaaa actacccttg tcacctttac actggttatt agctgataaa
     4501 gaaaaatcga tagtaattga aagtatgaaa gatggacttc atttatatga taatcctgtc
     4561 ggtgtgttga caaataatcc gccatttgat tatcaattat ttaatctgaa caattatcgc
     4621 tcgctatcaa atgga
//
LOCUS       DAYYAO010000143         4629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000143 DAYYAO010000000
VERSION     DAYYAO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.149"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACHARI_002726"
     CDS             complement(52..522)
                     /locus_tag="ACHARI_002726"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8443718.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACHARI_002727"
     CDS             complement(681..1139)
                     /locus_tag="ACHARI_002727"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8443719.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACHARI_002728"
     CDS             complement(1310..2455)
                     /locus_tag="ACHARI_002728"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8443720.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACHARI_002729"
     CDS             complement(2468..3385)
                     /locus_tag="ACHARI_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8443721.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACHARI_002730"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACHARI_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8443722.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1451 a          845 c          876 g         1457 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaa
//
LOCUS       DAYYAO010000144         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000144 DAYYAO010000000
VERSION     DAYYAO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.150"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHARI_002731"
     CDS             186..2855
                     /locus_tag="ACHARI_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8443723.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHARI_002732"
     CDS             complement(2946..3560)
                     /locus_tag="ACHARI_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8443724.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHARI_002733"
     CDS             3680..4219
                     /locus_tag="ACHARI_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443725.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHARI_002734"
     CDS             4216..4554
                     /locus_tag="ACHARI_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8443726.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1420 a          790 c          860 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAYYAO010000145         4525 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000145 DAYYAO010000000
VERSION     DAYYAO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.151"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..409
                     /locus_tag="ACHARI_002735"
     CDS             <1..409
                     /locus_tag="ACHARI_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8443727.1"
                     /translation="EIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGI
                     IEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNI
                     YGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            382..1059
                     /locus_tag="ACHARI_002736"
     CDS             382..1059
                     /locus_tag="ACHARI_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8443728.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1010..2191
                     /locus_tag="ACHARI_002737"
     CDS             1010..2191
                     /locus_tag="ACHARI_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8443729.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2204..3181
                     /locus_tag="ACHARI_002738"
     CDS             2204..3181
                     /locus_tag="ACHARI_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8443730.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3181..4089
                     /locus_tag="ACHARI_002739"
     CDS             3181..4089
                     /locus_tag="ACHARI_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFM8443731.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            4090..>4525
                     /locus_tag="ACHARI_002740"
     CDS             4090..>4525
                     /locus_tag="ACHARI_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8443732.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT         1704 a          546 c          844 g         1431 t
ORIGIN      
        1 cgaaattatc agaaaaatgt accagaaata tttaaaaacg gatattattt atattacgaa
       61 tttagagccg gaggaatacc tgactatatt aaaagagtgc aaatttatga tcggaaattc
      121 ttcttctggg ataattgaag ctgcatcatt caaaatacca gtaataaatt taggaaccag
      181 acaaaatgga cgagaacaat ctaaaaatat tataaatgca aatctaaatg aaaatgaaat
      241 tgaaactgct ttgaataaaa tacaagatcc tcagtttata aaaagcttga aaaatgttaa
      301 gaacatctac ggtgacggca acagttctag aagaataaca aaaacattga atcaaatatt
      361 gaatgatggg gaagatatta agtggattca aaaacaaata gcttattaaa atatactagt
      421 gatccataca ttattattgg ggctggcgga cacgcaaaag tcattattga aaccttaaga
      481 ttagaaaata agttaatata tggatttttt gatgataagc aaaataaaat gcaagatctg
      541 aaatatcttg ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa aaaaatctat
      601 aaatttattg tagctattgg tgactgtgta gtaagaaaaa agattataaa aaaattgaat
      661 atgccaagtg agcaatatgg aatagctgtt catccaagtg ctatagtggc aaaagaaatt
      721 tcaattggtt atggaactgt tatttttgcg aatgctgtaa ttaacccaga tgctgtaatt
      781 ggagagcatg ctataataaa cacaggtagc attgttgagc atgattgtag aattaataac
      841 tatgttcatt tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact
      901 caaataggaa ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata
      961 ggcgcaggga gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca
     1021 gctaaaatta agaaagaagg aattaatttt gaattctaga attttattat caagtcctca
     1081 tatgggagga acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc
     1141 attaggtgaa aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg
     1201 cggagcttct gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa
     1261 tgttggaaaa aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc
     1321 tattttatat caaggggctg taccagtgtt cattgactca gaacctaata catggaatat
     1381 gagccctgtt gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa
     1441 ggcaatcatc gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat
     1501 tgcaaaccaa tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta
     1561 taagggaatt tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa
     1621 gatcatcaca acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca
     1681 ttcaagattt ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat
     1741 aggttataat taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt
     1801 attgaatgat agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc
     1861 tggaattgat agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt
     1921 gtctacttta acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa
     1981 actaaatgaa aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt
     2041 atttcaaggg gtaaaaatgt attaccatga taataatctt agtgtgtcag aaaagttatt
     2101 taatagcggc ttatgcttac cttctggaag taatatgaca gatagtgaac aagaatttgt
     2161 tattgatgag tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca taggaattat
     2221 tggtttaggt tccatgggga aaagaagact tagactcatt caagaacact ttaatgatat
     2281 tttactatgt ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa aggaatttgg
     2341 aataccaact tataatagtc taacagaggc aaaaaatttg tttgaaataa attcagtttt
     2401 tgtttgtaca tcccctataa cccatgaatt tattataaaa gaggctttaa atttagattg
     2461 taatatcttt acggaaatca atttattaaa tgaatattat gatgaaataa tcgaagtagc
     2521 taaaaagaaa gatagattac tttatctatc ttctacattt ttgaagagaa aagaaattca
     2581 atatattaag tctaaagtga cgcataataa aaaaatttct tatcaatacc atgtaggaca
     2641 gtatttaccc gactggcatc cttgggaaga ttatcgcaat ttttttgttt caaacaaaaa
     2701 aactaatggg tgtagagaaa tattcgctat tgaattacct tggattatag atacatttgg
     2761 gaaagttaaa tcgtttactg taatgaccca gaaattatcc aatcttgata ttgattattc
     2821 ggacagtttc actcttatta ttgagcatga aactggtata gttggaactt taagtgtaga
     2881 tgttgtttca agagtagcta aaagaaattt aactataatt ggtgaaaaga ttttaataga
     2941 atgggaaggt aacccggatt ctctattttc atggaatgaa gaaacttcta gtatgacaga
     3001 tattcaattg tacgatgagg ttatgcatga taataattat tcacatacta ttattgaaga
     3061 tgcatattta gaggagatta aagaattttt cacgcttttg gaaaagaaaa tggaacccca
     3121 atatactttt gaaaaagata agtatattat taacttaatt aacgacattg aggtaaatta
     3181 atgatgaaag taattttttt tgggttaggg tcaattggaa agagacatct aaaaaatcta
     3241 ataagctatg gaaaaaagaa ttctattaat tttgatataa cagcttatag tagcagtgga
     3301 aaaaaagagt ttgaagaaat aacgtatatt caaaatagag acgatttgga tacacattat
     3361 gatatagctt ttattacaaa tccgactttt aagcatctgg aaacactaaa tgtaattgtt
     3421 gaaaaagttg attacatatt tttagaaaaa cctgcttttt ctgagagtat tgatcttact
     3481 caatataatt atgattacaa aaatatttat gttgctgctc cactgaggta taaaaatatc
     3541 atggaagttg ttcgtaatag tattcaaagt aaaaaagtat attctgtaag agctatttgt
     3601 tctacttatc ttcctgattg gagaaaaggt actgattata gaaataatta ttctgccttt
     3661 aaatctatgg ggggaggagt agaattagac tgtatacatg aattggatta tattataaac
     3721 ctatttggtt tccctaaatc agtaaaaaaa cagtttggta aatattcgaa tcttgaaatt
     3781 aacagtaatg atttagcaac atatatattg agatacgatg ataaaattat agaaattcat
     3841 ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt agatgaatgt
     3901 atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt taaagaaatg
     3961 cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga aaacgtagta
     4021 acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa aatagctgag
     4081 gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa ggggtttaaa
     4141 aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt agcaatgata
     4201 gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa tacggacagt
     4261 aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt agaacgtaaa
     4321 cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc attcttacaa
     4381 ctagaaagag aagataatat atttgattta attatagatt tagatttgac ttcaccttta
     4441 cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt agataaagac
     4501 gtctactttt cagttgtttc tgcaa
//
LOCUS       DAYYAO010000146         4486 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000146 DAYYAO010000000
VERSION     DAYYAO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.152"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACHARI_002741"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACHARI_002741"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8443733.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACHARI_002742"
     CDS             complement(1036..1956)
                     /locus_tag="ACHARI_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8443734.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACHARI_002743"
     CDS             2069..2821
                     /locus_tag="ACHARI_002743"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8443735.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="ACHARI_002744"
     CDS             3685..4218
                     /locus_tag="ACHARI_002744"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8443736.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1490 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttacc
//
LOCUS       DAYYAO010000147         4450 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000147 DAYYAO010000000
VERSION     DAYYAO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.153"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACHARI_002745"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACHARI_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8443737.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKRAQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACHARI_002746"
     CDS             complement(2017..2919)
                     /locus_tag="ACHARI_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8443738.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACHARI_002747"
     CDS             complement(2916..3608)
                     /locus_tag="ACHARI_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8443739.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACHARI_002748"
     CDS             complement(3651..4292)
                     /locus_tag="ACHARI_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8443740.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1376 a          949 c          768 g         1357 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtgc acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc
//
LOCUS       DAYYAO010000148         4405 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000148 DAYYAO010000000
VERSION     DAYYAO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.154"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            776..1162
                     /locus_tag="ACHARI_002749"
     CDS             776..1162
                     /locus_tag="ACHARI_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8443741.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1260..1451
                     /locus_tag="ACHARI_002750"
     CDS             1260..1451
                     /locus_tag="ACHARI_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443742.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1965..2297
                     /locus_tag="ACHARI_002751"
     CDS             1965..2297
                     /locus_tag="ACHARI_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8443743.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2207..2410
                     /locus_tag="ACHARI_002752"
     CDS             2207..2410
                     /locus_tag="ACHARI_002752"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8443744.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            2441..2587
                     /locus_tag="ACHARI_002753"
     CDS             2441..2587
                     /locus_tag="ACHARI_002753"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443745.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            2594..2749
                     /locus_tag="ACHARI_002754"
     CDS             2594..2749
                     /locus_tag="ACHARI_002754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8443746.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3025..4365
                     /locus_tag="ACHARI_002755"
     CDS             3025..4365
                     /locus_tag="ACHARI_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8443747.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         1533 a          809 c          742 g         1321 t
ORIGIN      
        1 tgaattcgga attaaaaacc acgataggta actttaaaca aacatgaaga gactttaata
       61 agacttcttt atctgaaaac acttttgttt ctctctttcc tttctacatc attaaatagt
      121 attttatacg ttatctaaaa tactattcct ctgtcaaaat aagctaactt acttcattat
      181 aaaatatcca cttatcgctt tcatgtatat tccaccgtaa aaagtacatt ttatcaccga
      241 gaactcatca tttttcatca tgatgaatac tatttacata tatctagttc aaagacaata
      301 attattatga tccgttatta tctaagcact atacagataa taggaatttt tcgtttctaa
      361 atactccaat ttgggaatcc aataaaaatt ttcgttcaaa tacgtgtcta ttcgaactct
      421 tttctagttg ttaattatga agggggaaga aataaaaaat acaatctata cacgaataag
      481 aatattatcc aaaaggaggt tttagaaaag atttattata cgtaaaaagc tcaagacatt
      541 ttcttagcca taaggacatg tttgatattt aagaagatat atgaattttc ttttctaagt
      601 ataaagataa ctatccatta tcaactaaaa aaacattttt gaaaataaac atcagcagtc
      661 ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc agcataattg cattagcttc
      721 ttttcgtgtt ttttgcctct tttcccttta aatcttaatg gaggaggtgt gcaatatgtc
      781 agataacgac gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg tcattttgaa
      841 caccgctaat acttattata aaaaacaaga agaaattcaa aaacatgaac aacttgatca
      901 actcccacca tatttgttta ctaaagatac tataaaattt aaacaaccga ttaatgtttt
      961 aaatgtgact ctccttgttg aagatataaa attagcaaaa atccttcctt tgttgaaaga
     1021 acctgaacaa atttttctta tcgaaaaatt tatctttaat aaaactgata aagaaattgg
     1081 aaaggttctg ggtattacaa gacagggtgc gacaaattta aaacatcgac tttacaaaaa
     1141 aattcaaaaa atgctggttt aatatctgca taataactaa ttttgtatgc ttctttgctc
     1201 gtcctcctcc ttccccaaaa aataaataga ttataagttc atgggaagaa ggtaaattta
     1261 tgaatacttt aaaagaagat ttcattttag caaagaatgg aaatgaagaa gcagttgtgg
     1321 ccattctcaa aagatttagc gctttggtcc ataagcaatc ctggagaact ggaaaatatg
     1381 atcaagattg ttatcaagaa tgtatgcttg caatatatct agctatctcg aaattcgaaa
     1441 ttaaagaata actcaactaa gggagtctgt gacataagta aaaagatagt ttcaaatttc
     1501 gaacaataaa tttgaagcca tcttctttat aattataaag tatcgatttt acagcgttct
     1561 ataatgattc atagaatcta aaacgaatga ttttaattat taacgttttt taattcgttt
     1621 ttagcttaaa aataactttt gtcacagtct cactcctaaa attactttat ttacgtacga
     1681 agtgggcagg aggtcgaatc cagaactggg ccatggtact tcaacgagga cttctcctct
     1741 agaattgaaa agcatgcgat ttaccttccg taacgacggt agatttattc aactatgtgt
     1801 ccaatcaaaa tttgtctgat ttcaaaaata gtcaagagaa ttttggttgt taaaaactct
     1861 tatctatcaa cttccacagt ttagttgaca aaccctgata ctaagttggg ctatgataca
     1921 cttttcttga ctatcagtta cgttaccgga aggaatgaac tctattgaaa aaaacatatg
     1981 atcgtaaatt taaactaaag atttcacagg atatccttga gaaaaaagta actaccaaga
     2041 aaattgctga agaatacaat atctctcgtc caacaatttc ccgatgggtt tcagaatatc
     2101 gtcgatacgg gaaaaatgct tttgctggac aaggaaaaag attacctgat aaagcagatt
     2161 tctacattct tgaacaggaa aataaacgac taaaagagga agacgagtgt tctacgaaca
     2221 caaagatcga tacggatctc cagggatcac tcaaaaactt tacgatgaag gaatcgaaat
     2281 caataaacgt gttgtagctg ttcttatgcg tgaaatgaat ttatgcacaa gaggatttca
     2341 tcgacgcaaa tcttctcatg gtagagaaaa aacaatcaaa gaaatggtaa aaaaaatgtt
     2401 gaatcgataa tttgaacaga accaaattga tgctgtttgg gtgacggata ttacttacat
     2461 tccatgtaat gacggtcgcc tctatttgtc cacctatatt gacttagcaa ctcgtattct
     2521 acgttactat atggtggatt cacacatgaa aaaagagatt gtcgtccgtc cattacaaat
     2581 ttattaagga gaattgccta cggtaattca ttccgataga ggtagccagc atcggtcttt
     2641 tatttatcaa gagctgttga ccgaccacca catcacacat tcaatgagcc aaactgggac
     2701 tcctgttgac aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag aattaatcga
     2761 ctccaacaaa tataaaagta aaatagagat gaaggtgtta atccaagatt atttagaaga
     2821 ctactaccca aacaaaagaa tccatacaaa atttatgatg actccttgac aatttgaagc
     2881 gaagaaaata agggatctat aatatctagt gttggtgacg ccttctgttg ccaatacttt
     2941 ttcttttttc taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa
     3001 aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat
     3061 attctagacc taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac
     3121 gaaacatgtt ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc
     3181 cactatcaaa ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg
     3241 aatgatgtca gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa
     3301 cactgtggaa agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc
     3361 cgcagagtca aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt
     3421 gcacgtatga agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct
     3481 aaaaccgttt ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg
     3541 gtgaaaaacg tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg
     3601 attgatattg tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct
     3661 agaaaagtcc gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca
     3721 ttggtgaaag agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa
     3781 cacatcggtc gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg
     3841 gatcgtcggt cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa
     3901 ttgaatggtc agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag
     3961 atagtcgatc ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag
     4021 gtttttcttt ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa
     4081 gactacaagg agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga
     4141 acagaaatca aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac
     4201 aaccatatca aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga
     4261 gaacgaatct ttttatatcg aggaaaatat ttcaagaaaa ctaagaacac tacccaactg
     4321 actaaagcca gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc
     4381 gattattcta ttgaatttct tcacc
//
LOCUS       DAYYAO010000149         4151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000149 DAYYAO010000000
VERSION     DAYYAO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.155"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACHARI_002756"
     CDS             <1..106
                     /locus_tag="ACHARI_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8443748.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="ACHARI_002757"
     CDS             complement(359..508)
                     /locus_tag="ACHARI_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443749.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="ACHARI_002758"
     CDS             970..1182
                     /locus_tag="ACHARI_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443750.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="ACHARI_002759"
     CDS             complement(1460..3259)
                     /locus_tag="ACHARI_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8443751.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="ACHARI_002760"
     CDS             complement(3383..3979)
                     /locus_tag="ACHARI_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8443752.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1226 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt t
//
LOCUS       DAYYAO010000150         4149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000150 DAYYAO010000000
VERSION     DAYYAO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.156"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..751
                     /locus_tag="ACHARI_002761"
     CDS             <1..751
                     /locus_tag="ACHARI_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8443753.1"
                     /translation="FNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEHAVKLR
                     KEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQEWNAQI
                     QKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHVDYFEP
                     CFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGSSFVYP
                     RTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            738..2179
                     /locus_tag="ACHARI_002762"
                     /pseudo
     CDS             738..2179
                     /locus_tag="ACHARI_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            2190..3521
                     /locus_tag="ACHARI_002763"
     CDS             2190..3521
                     /locus_tag="ACHARI_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8443754.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            3534..3992
                     /locus_tag="ACHARI_002764"
     CDS             3534..3992
                     /locus_tag="ACHARI_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8443755.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1381 a          611 c          885 g         1272 t
ORIGIN      
        1 ttttaattcg gatgattttg gctatagcca tggtgtgaat tatgggatta tggatgctta
       61 tcagcgagga attttgacat caactacatt gatggcaaat atgccgggtt tcgagcatgc
      121 tgtgaaacta agaaaggaaa tgcctaggct aggtgtagga gtccatttaa cattgacgtg
      181 tggcaaacca ctcctcaaaa ctgtcgatac tcttatcgaa agcggagagt tcagatcctt
      241 atcttattat cagcatccat ttaacattga caaagatcaa ctgtatcagg aatggaatgc
      301 tcagattcaa aaaatttatc atgcggggat cataccaact catttagaca gtcatcatca
      361 tactcatact ttggatgaaa atcaggaggt agttgttaca ttagcaaaaa aatatgattt
      421 gccggttcgg agcaactttg aacgaaaaga tgaagttcgt catgtcgatt attttgagcc
      481 ttgtttcgat gtcgtaggag aaactgatga aaaaaaacga caaatcaata cgagtgtgga
      541 agaatatttg gcaagtttac tcatgatatt gagagaagat aaaacgatcg aagtcatgtg
      601 tcacgcagcg tatttggatc aggaattgtt aaatggttca agttttgtgt atcctcgtac
      661 ccaccaagtc gaatttttaa ttcattcagc atttgctcaa aaagtgaaaa atgatccttt
      721 gattgagtta gtaacatatg aggatattta atagaggagg aataaaaatg aatgttaaat
      781 caaatgtgca gaagcttgga agagcgatgc tacttccagt tgcagcgatg ccattagcgg
      841 gattgatcat gcgtttatca gctgacgata tgctgaacat cccagtaatt ggtgcagctg
      901 gaaatgccgt ttttggaaac ttggatatct tatttgctat aggagttgca atcggttttg
      961 tcaaaaataa ggacaaagga atacctgcac ttactggttt tttagcgatt gctaccttga
     1021 aaaaaggctt agaaattatg gacccagaag tgaatatggg gatttttggt ggaattattt
     1081 ccggtttagt agcggcatgg acgtataatc gctttaaaga acaaaaatta ccagtagttt
     1141 tttcttattt tgcgggcgaa aaattcccat tgactatggt gatgattcta caaacaatcg
     1201 cagctgtttt tttgggcttt atctggccgc tcatccaaat tgggattgat aattttgcaa
     1261 aactgctcgt tgatatggga gcattaggtg tggggatatt tatgtttttg aatcgtttgc
     1321 tgatcccctt tggactgcac catgtgttga atacgtatat ttattacgat ttaggaagct
     1381 atacatctcc tagcaaggaa gttttcagag gagagatgac acgctttatc aacggagatc
     1441 ctcaggctgg actgttttta tcagtttttt tgtagtaatg atgtttggta taccagctat
     1501 atgtctagct atctataaag cagcttataa agaaaataaa gagatggtca aaggaatcat
     1561 gggaagcggt gcaggaactt catttattgc aaatatcact gagcctgttg aatttagttt
     1621 catgtttctt tctcctatgt tgtacgtgat ccatgcactt tttgccggtt tagcagggat
     1681 tgtctgttac ttattcaata tacgaattgg ttttacattc ggagcatgta ttgttgatta
     1741 tctcatcaat tttcgaatcg ctaccaatgc tattttgatt cttccaatcg gaattttctt
     1801 ttttgccttg tattacgtta ctttttatta tttgatcaat aagagaaaca ttcagacatt
     1861 aggacgtgaa gctaaggcag agtttggcaa tgaagtaacc ttagaagaaa cagagttagg
     1921 gttagcaagt aaaaattatg aatatatggc aacaaaaatg ctacaagcgt ttggaggaaa
     1981 agccaatatc ctggatgtct atagttgtaa tacacgccta cgggtagagg tagtggatcc
     2041 caccatggtc gaggaacagc gaatcaagca attagggata agcgggatca ttaaaccaac
     2101 tgaaaaaaat tatcaaatta tcatcggctt agaagtgact tatgttatgg cagaattcaa
     2161 taaattatta gaagaataga aaggaaaaga tgatgatgaa aaaacagatt atcactatag
     2221 ctggaggcgg gagtacgtat acaccaggaa tcgttcaagc cattttgaac aatgaaaaac
     2281 ggttgccatt atctgaaata cgtttatatg atattgatga agaaagaaat atggatatgt
     2341 acttgattgt taaatttatg ctgaaaagaa aaggtttttc aaatattcgc attcgtgcaa
     2401 cggatgatcc aaagcttgct tttacaggat gcgattttgt tttctcacaa attcgtgttg
     2461 gaggattgga gatgcgagaa aaggacgaaa agattccact taaacatgga ttagtaggac
     2521 aagaaacgtg tggactcgga ggatttgctt atgggatgcg ctcaatcaaa ggtttattgg
     2581 aaattgtcga tcatatacaa gactatgccc ccaaagcatg gattttgaat tatacaaatc
     2641 cagagtcgat cgtttctgaa gctgttcgtc gtaaatatcc gaatgtaaaa atgattaatg
     2701 cctgtgatat gactatttca attgaagaaa cgattgcagt gaattacggt tatgatcgta
     2761 agaattggat cgttacgtat tatggtttaa accactttgg ctggtataca tcgatttatg
     2821 ataaagaatt gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa
     2881 tgcaagtcgc cgattttaat ataggagata aaacttggca aaaaaccttt caaatgatgt
     2941 cagtcatcac gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat
     3001 atcctgatat ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg
     3061 atggacgaga gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag
     3121 aagtattgaa ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct
     3181 tgttgaatga tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga
     3241 atctacggtc agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggagc
     3301 caattacact aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg
     3361 cagtagagaa gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag
     3421 catttacatt gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa
     3481 tgatcaaagt aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt
     3541 tctttaaaac aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag
     3601 cagtaaaaga cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc
     3661 atgatggata tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac
     3721 atgccataac gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata
     3781 cagttgaact cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag
     3841 aagggacact gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg
     3901 tgatcattgt agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag
     3961 taaagattaa agatagttgt tttgaatttt agtgtacgat gaaaaaagtt aaatagggtg
     4021 atatttatgg gtgaatttac aaaatttttt tgaaatgata tgtttcaatc actagattta
     4081 atcattttga tttttgttaa ttatcgaatt tttttgttgc tcctatgatt tagaaaactt
     4141 attttcagg
//
LOCUS       DAYYAO010000151         4072 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000151 DAYYAO010000000
VERSION     DAYYAO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.157"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1435
                     /locus_tag="ACHARI_002765"
     CDS             86..1435
                     /locus_tag="ACHARI_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8443756.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="ACHARI_002766"
     CDS             complement(1563..2153)
                     /locus_tag="ACHARI_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8443757.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="ACHARI_002767"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="ACHARI_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8443758.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          745 c          789 g         1362 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa ct
//
LOCUS       DAYYAO010000152         4015 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000152 DAYYAO010000000
VERSION     DAYYAO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.159"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(175..1461)
                     /locus_tag="ACHARI_002768"
     CDS             complement(175..1461)
                     /locus_tag="ACHARI_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8443759.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1454..2722)
                     /locus_tag="ACHARI_002769"
     CDS             complement(1454..2722)
                     /locus_tag="ACHARI_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8443760.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2974..3435
                     /gene="mscL"
                     /locus_tag="ACHARI_002770"
     CDS             2974..3435
                     /gene="mscL"
                     /locus_tag="ACHARI_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8443761.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3494..3940)
                     /locus_tag="ACHARI_002771"
     CDS             complement(3494..3940)
                     /locus_tag="ACHARI_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8443762.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1136 a          817 c          707 g         1355 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaatac tggtttattt
      181 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
      241 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
      301 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
      361 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
      421 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
      481 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
      541 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
      601 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
      661 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
      721 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
      781 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
      841 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
      901 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
      961 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     1021 ttcatacatt tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt
     1081 gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt
     1141 cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg
     1201 tttcccaaat aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa
     1261 atgggcaatt ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt
     1321 atcgatcgaa ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg
     1381 caacaaatac acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg
     1441 ctcatattct gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt
     1501 cgatttgctc agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc
     1561 attcttcatc agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg
     1621 ccgcttgcgg agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag
     1681 acagttccaa ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat
     1741 gaagtacttg atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga
     1801 acgtatccac gctactagag gcataataag ctagactttc tttctctctg acattcatga
     1861 aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt
     1921 ctggttcatc gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg
     1981 gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg
     2041 gataaactgt ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg
     2101 gatcaacatc gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt
     2161 aggacgctag cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga
     2221 tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt
     2281 gtttatcttc cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg
     2341 tcaactgatc aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat
     2401 ctaccgcttg agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc
     2461 ctgcgtttag ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat
     2521 aaagctctgc aagttgcgca cttaactttg tctgatccgg ataattcaaa ctatttgttt
     2581 ccaacatgct gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa
     2641 aacggataaa aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc
     2701 cttctgccaa ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac
     2761 tcttttaatg ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca
     2821 ggcaattcgc cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt
     2881 atcaaaagaa acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat
     2941 actaaagaca aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc
     3001 atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc
     3061 atcgtaaacc aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg
     3121 atcacaggta cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa
     3181 ggcgtcacat ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt
     3241 tttgtcttat tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa
     3301 gagcaagtgg aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat
     3361 cttcttgcca aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc
     3421 gatacttcta aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc
     3481 tttttttaga ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat
     3541 cttccaaata cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt
     3601 attcttgtgc gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg
     3661 tttcttgaac aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac
     3721 gtccaaaatg gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat
     3781 cagtatcttg aaaaaacgta tgccaagctt gtccatcaat gtcatcaact tcttgataag
     3841 gacactgttg ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg
     3901 ctgttgacaa ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt
     3961 ttttattctt agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatc
//
LOCUS       DAYYAO010000153         4002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000153 DAYYAO010000000
VERSION     DAYYAO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.160"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..258
                     /locus_tag="ACHARI_002772"
     CDS             <1..258
                     /locus_tag="ACHARI_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443763.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="ACHARI_002773"
     CDS             260..397
                     /locus_tag="ACHARI_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8443764.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            434..727
                     /locus_tag="ACHARI_002774"
     CDS             434..727
                     /locus_tag="ACHARI_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443765.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            724..948
                     /locus_tag="ACHARI_002775"
     CDS             724..948
                     /locus_tag="ACHARI_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8443766.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            945..1964
                     /locus_tag="ACHARI_002776"
     CDS             945..1964
                     /locus_tag="ACHARI_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8443767.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            2153..2226
                     /locus_tag="ACHARI_002777"
     tRNA            2153..2226
                     /locus_tag="ACHARI_002777"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2187..2189,aa:Ile,seq:cat)
     gene            complement(2741..3040)
                     /locus_tag="ACHARI_002778"
     CDS             complement(2741..3040)
                     /locus_tag="ACHARI_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8443768.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(3046..3276)
                     /locus_tag="ACHARI_002779"
     CDS             complement(3046..3276)
                     /locus_tag="ACHARI_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443769.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(3535..3648)
                     /locus_tag="ACHARI_002780"
                     /pseudo
     CDS             complement(3535..3648)
                     /locus_tag="ACHARI_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1388 a          651 c          730 g         1233 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccatta tgcattacag acgaagaagc
      361 agataaaatc attaataaag ataagagcgc atcttaattg atgcgttttt atttaaggta
      421 aaggagttgt cacatgatta atttagggga atggggaatg atagcaggat caataaccgc
      481 tatcgtttct ttgattttat tagtaattag accgattgct gcatctttct cgaaaattac
      541 tgagactctt tcaaaagtaa gccacaattt agatttgctg actaaagatt tagaatcgag
      601 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
      661 acatacagaa aaattggtgg aacacacgca acaaatcaaa actttgttta gggagagaag
      721 aaaatgaata ataaaacgtt cgaagtacta aaatggttcg cactggtaat tattcccgca
      781 ctagctactt tcgtggggtt agttggtaaa gcgctcaatt ggcagtacac agatatctgt
      841 gttgtcatca ttactggttt tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga
      901 acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta
      961 ctattactgc gatgagcctg ttaacggctc tttttttatt gccaattaac ggatttgcct
     1021 atactatcaa caatgaattt aatttgggcg caaatgaagg tagctcacaa gtagcaaata
     1081 atcagtatat tttactgcat gaaacggcta atgaaacagc aacaggacgc aatgaagcgc
     1141 agtatatgca acgttcatgg actagcgctt atactgctta tattgtggga gacggcggaa
     1201 ttgtttatca agtcggtcaa cctggttatg tacagtacgg tgctggttcg tatgctaatg
     1261 ccaacagtcc tgtgcagatt gagttacaac acacacatga taaagcaacg tttgagaaaa
     1321 actacaaggc atacgttgaa ttggctagag attcagcaat gaaatatggt attccattaa
     1381 cgttggacac tccttataac caaccgggaa tcaaatcgca tttatgggta acacaaaaca
     1441 tctggggcga tcatacagat ccttacggtt atctttctga aatgggcgta agtaaagaaa
     1501 aattagcata tgatttagct catggattta ccgatgaaaa tccaacaact tctgaaaaca
     1561 agcctgtcat tgatccaaca cgagctggtg cagctaatcc tacactgaca gatggaacga
     1621 attacgccca cattgatcag tttggagaaa tcgaaaatgc aaatttgcat gtagctggat
     1681 ggcacattgc taactataaa tacgagtata tcttcattat ggattacaat actgggaaag
     1741 aattagctcg agtaagagct gatggaattt atagagcaga tgtaaatcaa gcttataata
     1801 cttctggaaa tgttggctat catgtatctt ttaacatgcg taacttccct aataagaaag
     1861 tctatgtcat gatgcgggca acgaatgatc cagagggaaa cactaaaggc ggtgcgcaag
     1921 atttccatga caaacgttgg tatttaaata ttcctaaacg ataaaaatag ctcctcgttg
     1981 aggagcatta cataactata ttgacaacta taaaaatcat tcgataaaat agtgatgtta
     2041 tcgcatatct tcactatcac ccataatagt cacactccaa gctatgcgat aacaggtttg
     2101 ttgccacaca ttctactggt tgattgttta tggctttatg tggcaacaac cagtaccctt
     2161 agctcagttg gtcagagcag acggctcata accgtccggt cgtaggttcg agtcctacag
     2221 ggtacattaa cgtagccatt tgaatcgttc tgtgttagaa ttttttgaag agtattatac
     2281 aagctaaagc ttttcttcat tgccactcaa atgagtggct tttttatgta tccttttatg
     2341 gattaatgaa aggatgtttc acatagttat atttctgtat atttgaaaag ttttactttg
     2401 atttttaaat agaaagacat ttgggttata ttgtgagata ataataaaga agagtttaaa
     2461 gcgttcccca aaaaccactg ccccataagt gtgttacgct ttaaactctt ttatatttga
     2521 agccattaaa aagcatacca tatttttgaa aaaaagtgag aaaaaaggct tataattgga
     2581 gtggtagtta attagtgact tatttttgat tttatagcac tgatactata aaatatagat
     2641 atcatcatat tacacaatct taatactaac ttaaaaaata tctcctttca taagtatggt
     2701 gataaaatcc gttccgggct acctttttag gtagcctact ttaatctttg tatctttctg
     2761 gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgcttcga gcgtctagca
     2821 catcagtcac gatatcaaac aaaaaatata cgtctttctt cattctagtt ttcgcagcag
     2881 ggattttaaa gtagttctta ttagaatagt agagattaat taataagcta tcttcgattg
     2941 ctaaaaagaa aacttctgaa tcccacacct tataaaaatc tttgacaaat ctattcgaag
     3001 gatcaaattt aaaccataat tgcgtctcat taaaaagcat aaccattact ccgatctatt
     3061 tttaaactta gtctctacct ctaatatata tcgagttttt attttgcctt cagagaatac
     3121 tgtttctttt tttgcagtta caggttgttt attttcggaa aaagctaata tagctaaaat
     3181 tgaaacatcc atctggaatt tgtctttttc gttgctttgc tcataaaaat ctgcatattc
     3241 atcactaata ttttttctaa taaattcttc catcataaag atcacctcga aaagagtata
     3301 cgaacaaacg ttctttttgt aaaggtggaa tttattatac taaataaata ggtgaacaag
     3361 tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa taaaacacaa acaaaaagaa
     3421 atcctattat gataacattt cttataataa caaacacaac agaagacgtg taatagttag
     3481 tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt
     3541 agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac
     3601 tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa acagtcgaag
     3661 agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat
     3721 tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca gtcctatcca
     3781 acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat
     3841 gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta
     3901 caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc gaaataaagg
     3961 atctaagcca atagcttaga tcctttattc tatgctttat at
//
LOCUS       DAYYAO010000154         3923 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000154 DAYYAO010000000
VERSION     DAYYAO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.161"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACHARI_002781"
     CDS             complement(573..1130)
                     /locus_tag="ACHARI_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443770.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACHARI_002782"
     CDS             complement(1309..1920)
                     /locus_tag="ACHARI_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443771.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACHARI_002783"
     CDS             complement(2033..2290)
                     /locus_tag="ACHARI_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443772.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACHARI_002784"
     CDS             complement(2887..3165)
                     /locus_tag="ACHARI_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443773.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACHARI_002785"
     CDS             complement(3199..3450)
                     /locus_tag="ACHARI_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443774.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DAYYAO010000155         3864 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000155 DAYYAO010000000
VERSION     DAYYAO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.162"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..1510
                     /gene="gndA"
                     /locus_tag="ACHARI_002786"
     CDS             89..1510
                     /gene="gndA"
                     /locus_tag="ACHARI_002786"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8443775.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            1703..2389
                     /locus_tag="ACHARI_002787"
     CDS             1703..2389
                     /locus_tag="ACHARI_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8443776.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            2386..>3864
                     /locus_tag="ACHARI_002788"
     CDS             2386..>3864
                     /locus_tag="ACHARI_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8443777.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFI"
BASE COUNT         1305 a          667 c          827 g         1065 t
ORIGIN      
        1 aatatgttgt tctttagttt gcaatgtagc cgtgttaagc tataatgtaa gagattaggg
       61 agaaatcccg tatgtaggag gagttcatat gtcaaaacaa caaatcggcg ttgtcggtat
      121 ggccgttatg ggtaaaaatc tagccttgaa catagaaagc cgcggctatt cagtggcttt
      181 attcaatcgt acaggggcta aaacaaccgc tgtagtagaa gaacacccag ataaaaattt
      241 caaagcaaca tatacaatcg aagaatttgt agaatctatc gagaaaccac gtcggatctt
      301 gctaatggtc aaagcaggac cggcaacaga tgcaacaatc caagagttac ttcctcacct
      361 tgataaaggc gatattttga ttgacggagg aaatacattc tttaaagata cgatgcgtcg
      421 taatgaagag ttagccaact ctggcatcaa ctttatcggt acaggcgttt ccggtggtga
      481 agaaggagct ttaaaaggac cttcaatcat gcctggtgga caaaaagaag cctatgcgct
      541 agttgcacca atcttagagc aaatttctgc aaaagcagaa gacggagcac catgtgtcac
      601 atatattggc ccaaatggag ccggacatta tgtgaaaatg gtacacaacg gtattgagta
      661 tggagatatg caactgattg ctgaatctta tgacttaatg aagaatattt taaacctttc
      721 agtagaagaa atggctgata tctttaaaga atggaatcaa ggcgaattag acagctattt
      781 gatagaaatc actgctgaca ttctgacacg taaagacgat gaaggaacag gtaaaccagt
      841 agtagatgtg atccttgatg cagctgggaa taaaggaaca ggtaaatgga caagccaaag
      901 cgcgcttgac ttaggtgtgc cacttccatt gatcactgaa tctgtatttg cccgctatat
      961 ttctgcttat aaagaagaac gcgtacaagc aagcaagatt ttgtctcgta cgaacgattt
     1021 tgagttcacg ggagacaaaa aagaattggt cgaaaaaatc cgagaagcgt tgtatttcag
     1081 taaaatcatg agttatgccc aaggatttgc tcaattacgt gtcgcttcaa aagaatttga
     1141 ttgggacttg ccatttggtg aaatcgcaaa aatctggcgc gctggatgta tcatccgagc
     1201 tcgattcttg caaaaaatta cggatgccta tgacaaaaat cctgagatcg aaaatttatt
     1261 gctagatgat tacttcgttg aaatcacgaa aaaatatcag caatcagtca gagacgtcgt
     1321 tgccttggct gtccaagctg gcgtacctgt accgactttc tcatcagcca tcgcttactt
     1381 cgattcatac agagcagaac gtctgcctgc aaatataatc caagcccaac gcgattactt
     1441 tggcgcacat acctatgagc gtgtcgacaa agaaggaatc ttccattact catggtacca
     1501 tgaagaataa tagtctttcg gctgttagga aatgagaaaa taaaaagagg acgaaagagc
     1561 tgaagtcgat tgttatgagt agacttcagc tcttttttaa aattgctatc catgataaaa
     1621 tgactataga tttttaagca tattatcggt ataatgatag agtttttaaa aagccagtag
     1681 aatagaaagg ataaaataat aaatgagcaa tattctaatc attgaagatg aaaagaactt
     1741 ggcacgattt gtcgagctag agttgaagca tgaagggtat aacgcagaag tccactacaa
     1801 cggccgaact ggtttagatg cggcattaaa taatgagtgg gatgccatat tgttggatct
     1861 gatgctccca gaattgaacg gtttagaagt ctgcagacgt gtacgccaag tcaaaaatac
     1921 accaatcatt atgatgactg caagagattc tgtgatcgat cgtgtatctg gacttgacca
     1981 cggagcagat gactacatcg tcaaaccatt tgctatcgaa gaattattag cacgtcttcg
     2041 tgcattgttg cgtcgaatcg atattgaagg cgataaaaat gtggctaaac aaactactat
     2101 tacttatcgc gatttgacta tcgaaaaaga aaaccgtgtc gtacgtcgcg gaaatgaagt
     2161 aattgaacta acaaaaagag aatacgagtt gctgcttact ttaatggaaa acgtgaacgt
     2221 ggtattagca cgtgacgttt tactaaataa agtgtgggga tacgaaacag aagttgaaac
     2281 aaacgtagtg gacgtatata ttcgttatct tcgcaacaaa attgacgttc ccggggagga
     2341 aagctatatc caaactgtac gcggaacagg ttatgtgatg cgctcgtgat aaagccgaaa
     2401 aaatcggaaa aaagagaact gaaagggccc tccttgacaa tcaagtgggc ctttgcgagt
     2461 tctttcttca tattcgttgt gtttacaatt ttcgctgtca ttacctataa atcatctatc
     2521 aatctgattg tggcaaaaga aagaaacaat gtagagcgta cgatttcaga agtagcttct
     2581 cgtttggcta actcggatga agaactgaca ttagtaaata cgtatcgtaa tctgacgaaa
     2641 gcgacaaatg acggagatga ggtcgtagat catacttcac tggtcgaagg aaacttaatg
     2701 aagatcgatt cctttatagc tgagctaggg cagccatcaa tggatcttta tgtttatgat
     2761 ttggacgaga acttgatttt taaaacccat gaaaacagtc gaaacctgat tcagactaca
     2821 cgtaaagttc caacgatcgt tacactagaa ggaaaaactg gctttttatc tatccagcct
     2881 atatattcta aaggtacaag agaaaagatc ggctatgcgc aaagcttcta tgaattatct
     2941 tctttctatg atattcgaaa taagctatta ttgactctta ttgttttaga agttgtttct
     3001 ttgatcttaa gtagtatttt aggtttcttt ttatcttctt atttcttaaa accactcaag
     3061 gtactcagag atacaatgga tactatacgt aaagatccgc aatcagatat ccacatgcct
     3121 gaaatcgaca caaatgatga attggcagat ttagctgaaa tattcaatga gatgcttgac
     3181 cgtatgcgtt tgtatattga acaacaagag caattcgtgg aagacgtatc tcatgagtta
     3241 aggacacctg ttgcaattat tgaagggcat cttagtctgc taaatcgttg ggggaaagat
     3301 gatcctgaga tattagaaga atcattaagc gccagcatgc aagaaatcag ccgtatgaaa
     3361 agcttagttc aagagatgct agacttatca agagcagaac aagtagatgt tcattatgcg
     3421 aatgaaacaa ctaaaggaaa agaagtcacc tatcaagttt ataataattt caagatatta
     3481 tatccagact atgtgatcac tttagatgat gatttgacaa aagaagtgac cctgcaaatt
     3541 tatcgtaatc actttgaaca attaatcatt atcatattgg ataacgcagt aaagtattca
     3601 actacacgaa aagaagtcca tatctctatt tcttccactc tgaatgaatt tgagatcgcg
     3661 attcaggact ttggcgaagg aatacccaaa gaagacctta ataagatctt caatcgtttt
     3721 tatcgagtgg ataaagcaag agctagaacg aagggtggaa atggtctggg gttgtctatc
     3781 gctaaacaac tggttgaaaa ctataaagga cgtatcttag cagaaagtgt agtagggcaa
     3841 gggacaattt ttagaatctt tatt
//
LOCUS       DAYYAO010000156         3770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000156 DAYYAO010000000
VERSION     DAYYAO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.163"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHARI_002789"
     tRNA            12..84
                     /locus_tag="ACHARI_002789"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHARI_002790"
     tRNA            88..160
                     /locus_tag="ACHARI_002790"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHARI_002791"
     tRNA            168..249
                     /locus_tag="ACHARI_002791"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHARI_002792"
     tRNA            267..339
                     /locus_tag="ACHARI_002792"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHARI_002793"
     tRNA            351..422
                     /locus_tag="ACHARI_002793"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHARI_002794"
     tRNA            439..524
                     /locus_tag="ACHARI_002794"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHARI_002795"
     tRNA            535..608
                     /locus_tag="ACHARI_002795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHARI_002796"
     tRNA            618..691
                     /locus_tag="ACHARI_002796"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHARI_002797"
     tRNA            711..783
                     /locus_tag="ACHARI_002797"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHARI_002798"
     tRNA            811..884
                     /locus_tag="ACHARI_002798"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHARI_002799"
     tRNA            898..971
                     /locus_tag="ACHARI_002799"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHARI_002800"
     tRNA            995..1084
                     /locus_tag="ACHARI_002800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHARI_002801"
     tRNA            1097..1170
                     /locus_tag="ACHARI_002801"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHARI_002802"
     tRNA            1189..1264
                     /locus_tag="ACHARI_002802"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHARI_002803"
     tRNA            1266..1336
                     /locus_tag="ACHARI_002803"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHARI_002804"
     tRNA            1350..1423
                     /locus_tag="ACHARI_002804"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHARI_002805"
     tRNA            1448..1535
                     /locus_tag="ACHARI_002805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHARI_002806"
     tRNA            1557..1628
                     /locus_tag="ACHARI_002806"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHARI_002807"
     tRNA            1651..1740
                     /locus_tag="ACHARI_002807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHARI_002808"
     tRNA            1753..1826
                     /locus_tag="ACHARI_002808"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHARI_002809"
     tRNA            1845..1917
                     /locus_tag="ACHARI_002809"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHARI_002810"
     tRNA            1921..1993
                     /locus_tag="ACHARI_002810"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHARI_002811"
     tRNA            1998..2068
                     /locus_tag="ACHARI_002811"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHARI_002812"
     tRNA            2081..2154
                     /locus_tag="ACHARI_002812"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHARI_002813"
     tRNA            2166..2237
                     /locus_tag="ACHARI_002813"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHARI_002814"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHARI_002814"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8443778.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
BASE COUNT          961 a          783 c          975 g         1051 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa
//
LOCUS       DAYYAO010000157         3725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000157 DAYYAO010000000
VERSION     DAYYAO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.164"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..838
                     /locus_tag="ACHARI_002815"
     CDS             86..838
                     /locus_tag="ACHARI_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8443779.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            835..1749
                     /gene="pfkB"
                     /locus_tag="ACHARI_002816"
     CDS             835..1749
                     /gene="pfkB"
                     /locus_tag="ACHARI_002816"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8443780.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1767..3698
                     /locus_tag="ACHARI_002817"
     CDS             1767..3698
                     /locus_tag="ACHARI_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8443781.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1198 a          664 c          850 g         1013 t
ORIGIN      
        1 tataattttt atgaacgttt ttgactgatt tatattgcta gtagaaaaaa aatatgctat
       61 tatttgtatt gaggtgatcg tgaaaatgct tacagaggaa cgcagacaaa aaatcctaga
      121 cttgcttgaa cagcagaaaa tcgtgaaatc acaagatctt gtaaaccttt tgaatgcttc
      181 tgaatcaacg ataagacgtg atttacaaga gctggagaat agtgggtttc tagaaagagt
      241 tcatggcgga gctaagaaag agcaattatt gaattttgag cagaatatga gtgaaaaatc
      301 actcaaaaac gttcacgaaa aacagcaaat cgccaaatcg gcagctgaaa ccattaatga
      361 cgaagatgtc atttatttag acgctggctc tactacgtta gaaatgattc cttttctgca
      421 aggaaaacag atcacagtag tgacaaattc cgttcaacat gcggcaaatt tagttgattt
      481 ggagcttgct accattattt taggtggaac gataaagctg gcaactaatg ctgttttagg
      541 atctaatgcg atagaacaac taaaaaattt tcattttaat aaagcattta tgggaatgaa
      601 cggtgcccat cttgaacaag gattcacgac tccggatcca gaagaggcag ctgttaaacg
      661 tttagccatg aaaaattccg aagagacatt tgtattgatt gatcatacca aattcaacca
      721 gttgtccttc acctctgttg cacctttgga agcagccacg atcataaccg agcgttgtcc
      781 attagaattc gttaatgaat tccgtgaaaa gacaacaatt aaggaggcaa tacaatgatt
      841 tacacagtaa cacttaatcc atcaattgat tatatcgtac gcgtagatgg gttaaaacta
      901 ggcgatttga accgaatgaa ggaagacttc aagttacctg gaggaaaagg gatcaatgtt
      961 tcccgtattt tgaaacggat cgattctcaa tctacagcac tagggttttt aggagggttc
     1021 actggcgaat ttatttcgaa gtggcttgaa aaagaacaaa tccagtcttt gttcacaacc
     1081 gtaaaagaag atacaagaat caatatcaaa ctaaaatcag acactgaaac tgagatcaat
     1141 ggcttaggcc catctatttc agatgaagaa atcgatgagt tgaagcaagc attaggaaca
     1201 gtaggacaag gagatatcgt cgtattgtca ggaagcgcgc cagctagcct gcgaaaagga
     1261 ttttatcaag aactgattga tatcattcga gaaaaaggtg cagatttcgt gattgataca
     1321 acaggtgatg acctaaaaaa tgcacttgtt aagaaaccgt tgttgatcaa accaaacaat
     1381 cacgaattag ctgagcttta caacacaacc ttccaatctg tagaagaaat cattccgtat
     1441 gggaaaaaat tactagctga aggggcacag aatgtgctga tctcaatggc tggagacggt
     1501 gctttgctct tcacggaaca aggaacttat aaaacgaatg tattagttcg cccattgaaa
     1561 aattcagtag gtgcagggga ttcaatgatt gctggcttta ttggggctta cagccgtacg
     1621 aaagatccaa tcgaagcctt taaatgggga gttgcttgcg gaagcgcaac agctttttct
     1681 gatgacttgg cagctcgcga attgattgat gagttgctgc cagaagttga aatcacaaaa
     1741 attgattagt ttcaaggagg ataatcatga atatcaagga tttaatggtc aaagaagcga
     1801 tgatcatgga tttgcaagcg actgataaaa aaggcgcaat cgatgaaatg gtccaaaaaa
     1861 tgtttgatgc cggccggatt tccgatatcg aaacatataa agaaggaatt ctagcccgcg
     1921 aagcacaaac ttctacaggt cttggtgatg gaatcgccat gcctcacgcg aaaaacagtg
     1981 cagttaaaga agcaacagtt ttattcgcaa aaagccaaaa aggagtagat tatgaagcct
     2041 tagacggaca gccaacttat ctgtttttca tgatcgcagc tccagaaggc gcaaacgaca
     2101 cacatttgca agcattagca gcactatctc gattgctgat tgatgcagat ttcgtaggaa
     2161 aattaaaaga agcaaataca ccagaagaag tccaagactt gttccaaaca gctgaagagc
     2221 aaaaagaagc agaagaaaaa gcagaacaag aaacacaagc tgcagaagct tctgctcatc
     2281 cagaaagaaa attcgttgtt gccgttacag cttgtccaac agggatcgct cacacttaca
     2341 tggcagaaga tgctttgaag aaaaaagcga aagaaatggg tgtggagatc aaagtcgaaa
     2401 cgaacggttc agaaggaatc aaaaaccgat tgactgctga agacatcgcc cgtgcagaag
     2461 gtgtaatcgt tgcagcagac aaaaaagtag agatgaatcg ctttgacgga aaagaactga
     2521 tcaatcgtcc tgtcagtgat ggtatccgta aaccagaaga gttgatcaat ctagccctta
     2581 gtggtactgc accagtattc catggtgatg gaaaagaaag cagttcagaa gaaggaaatg
     2641 cggatggtac tatcggacaa agaatttaca aagatttgat gaacggcgta tcacacatgt
     2701 tacctttcgt tattggtgga ggaatcgcta ttgcgctttc tttcatgatc gaccaattta
     2761 ttggcgtgcc acatgatcaa ttatcaaatc tagggaatta caatcaagct gctagctggt
     2821 tcaaccaaat cggacaagct gcatttggtt tcatgctgcc ggtattagca ggatttattg
     2881 cttcaagtat cggtgaccgc cctggtttga tcgttggatt tgctgctggt gcattagcta
     2941 atacaggcgg tgcaggattc ttaggtgcac tgattggtgg attcttagct ggttatgtca
     3001 tcgtattttt acgtaaatta ttcaagaact taccaaaatc attggatgga attaaaacga
     3061 tcctctttta tccagtgttt ggcttactga ttactggttt cttgatgttg ctggtcaatg
     3121 tccctatgaa agcaatcaat gatgcattga acggtttctt gacaagtatg agtggttcga
     3181 atgctgcttt acttggtgca ttattagctg gaatgatggc ggcagattta ggcggtccaa
     3241 tcaacaaagc cgcttatgtg tttggtacag caactttagc tacgactgtt gcaactggcg
     3301 gaagtgtcgt gatggcttct gttatggctg gaggaatggt acctccattg gcaattttcg
     3361 ttgctacacg tttgtttaag aataaattca gcaaaacaga tcaagatgct ggtttgacga
     3421 acatcgtgat gggactatcg tttgtcacgg aaggatcgat tccatttgca gcagctgacc
     3481 cgattcgtgc cattccaagt tttattattg gttctgcttt aactggcgga ttagtcggag
     3541 ctttcggaat caaattactg gctcctcacg gcgggatttt cgttgtattc ttactgagtc
     3601 acccggtcat gtatttggtg tttattgcta tcggagcagt cgtatctggt gtgatttatg
     3661 gtttattgag aaaagctcct gcagtagcag aagcatagaa ataaaaaaga agaggctggg
     3721 ttttc
//
LOCUS       DAYYAO010000158         3308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000158 DAYYAO010000000
VERSION     DAYYAO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.165"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..804
                     /locus_tag="ACHARI_002818"
     CDS             <1..804
                     /locus_tag="ACHARI_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8443782.1"
                     /translation="AKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETY
                     TGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKID
                     ESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIG
                     IFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGS
                     LINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            814..1470
                     /locus_tag="ACHARI_002819"
     CDS             814..1470
                     /locus_tag="ACHARI_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8443783.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            1470..2846
                     /locus_tag="ACHARI_002820"
     CDS             1470..2846
                     /locus_tag="ACHARI_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8443784.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            2976..>3308
                     /locus_tag="ACHARI_002821"
     CDS             2976..>3308
                     /locus_tag="ACHARI_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8443785.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1306 a          376 c          582 g         1044 t
ORIGIN      
        1 gctaaacaaa acaatctaaa attaggtgat gaagttgatc ttgaattact agatattgaa
       61 aaaagtggaa aaataaaaag tcataaattt aaaattatag ggatcttttc tggtaaaaaa
      121 caggaaacat atacaggatt atcttctgat tttagtgaaa atatggtgtt tgtagattat
      181 tcaacaagcc aagagatatt aaacaattca gaaaataatg aaattgcaaa taaaatttta
      241 atgtattctg gtagtgcaga atctacagac ttagccttaa acaaattgaa agagcttaaa
      301 attgatgagt caaagtattt tgttgaaaaa gattctaatg cgtttgaaga gtctttagag
      361 tcagtgagtg gaataaaata tataataaaa gtaatgactt attctattat gttgggtggg
      421 atggttgttc tttcattaat cttgattcta tggttaaggg aaagaattta tgaaataggt
      481 atattcttat ctattggaac atctaagata caaattataa tgcaatttat attcgagtta
      541 atattcatat cgataccaag tataatatct tccttatttt taggaaatgt attactaaaa
      601 gtaattgtag atggattcat taactcagag gattcaatga tttccggtgg aagtttaata
      661 aataatagta gttttatgtc aaatataaca acacttggac aaagttattt aatattaata
      721 agtattattg ttttatcagt tgtatttgct tcttcattaa tgttaatcaa gaagcctaaa
      781 gaaatcttat caaaaataag ttaggagaaa ataatggata tattagaaat aaagaatgta
      841 gcatatagtt atgcaaattc taaagaaaaa gttttgtcag gagtaaatca aaaatttgaa
      901 cttgggaagt tttatgcgat agtaggaaag tcaggaacag gaaaatctac acttctttcc
      961 ttacttgctg gacttgataa acctcaaaca ggaaaaatat tgtttaagaa tgaagatata
     1021 caaaataaag gatatagtaa tcatagaaaa aataatatat ctttagtatt tcaaaactat
     1081 aatttaatag attatttatc accaattgaa aatattagat tagtaaataa atcagcagat
     1141 gaaagtatct tgtttgaact aggtttagat aaaaaacaaa taaaaagaaa tgttatgaaa
     1201 ttatctggtg gacagcaaca aagagtagct attgctagag cattggtatc agatgctcca
     1261 ataatactag ctgatgagcc tactggtaat ctagacagtg ttactgctgg agaaataatt
     1321 aatatattaa agacattagc taaagataga aataaatgtg taatagttgt aacacatagt
     1381 aaggaagtag cagattctgc ggatatcatt ttagaactaa gtggtaaaaa gttgaaaaaa
     1441 gtaaataaaa tgaatttgga ggttgaataa tgataaaaaa tgcatttgct tatgtaacta
     1501 gaaaaagttt aaaatcatta attatcatat tagttatttt atctatgtca actttaagtc
     1561 tcattagttt atctattaaa gatgctacgg acagagcttc aaaagaaaca tttgctaata
     1621 taacaaatag tttttctatg gagataaata gacaagtaaa tccaggaaca cctagaggtg
     1681 ggggaaatgt aaaaggtgaa gatattaaaa agatatctca aacagatagt atagactcct
     1741 atgtaaaaag aataaacagt gttgcagatt tagttgatta tgatattatt gaaactcaag
     1801 agactcttgc gaatcaatca cctgaaagag caaagaattt taaaagaaca gttatgttaa
     1861 ctggggttaa cgactcaaca aaagaaacaa aatttgtatc agaagcatat aaattagtag
     1921 aaggaaagca tttagaaaat gaagataaaa ataaaatctt aatgcataaa gatttagctg
     1981 aaaaaaataa ccttaaagtt ggagataaga taaaaataaa atctaattta tttgatgctg
     2041 ataatgagaa aggtgcagat gaaacagtag aagtagaaat taaaggtcta ttcgatgggc
     2101 ataatagcgg tggagtaagt gcagcacaag aactatacga aaatacatta atcactgata
     2161 ttcatacagc tgctaaagtt tatggtaata ccgaagatac agctgtatac caagatgcaa
     2221 cattctttgt aaaaggtgat aagaatcttg atagtgtaat aaaagatctt gggaaattag
     2281 atataaattg gagagaatat aatttgatta aaagttcatc aaattaccct gcattacaac
     2341 aatctatatc aggtatttac tcaatttcaa ataaattatt ttttggctca ttaatatttg
     2401 caggagttgt agtttcatta ttattattct tatggatgaa tgctagaaag aaagaaatag
     2461 cagtattact atcattaggt atatcaaaat tagaaatttt tggtcaattc ctaattgaga
     2521 tggtatttat atcaatcccc gcattcgttg gttcctattt cttagctcaa tatacagctg
     2581 acaagcttgg gaataatata ttgaataagg ttactggtga tatagctaaa caaatagcta
     2641 aacaatctgc atctagccaa ttaggtggtg gggcagaagc tgaaggattt aacaagacat
     2701 tatcaagttt agatatcaat gtattaccta aattcatgat ttatgtagtt atatttatga
     2761 gtttagtact tcttgtatca ttgattattt cttcattcaa tatattaaga aggaatccaa
     2821 aagaattatt aattgacaat aattaaaatt ttcggtgctt tttagcacca aaaatgagag
     2881 tgtaatataa accgtgtaag taaccatcaa tcctaggcgg gtggttgctt cacggttttt
     2941 atttttctaa aataagatca agaggtgatt acacgatggc tagaaagaaa agaaatcctg
     3001 atgctgagaa gttagctgaa tccattttga atacttatca acccgaatct gtagaggata
     3061 tgcaagatgt tttaaaagat gtgtttggtc ccttatttga aaaaatgctt caaggagaat
     3121 tgaataacca tttagggtat gacgcccatt ctaaagagcc aaagaaacat gacaatcgac
     3181 gaaacggtta tggcaataag acgcttaaaa cgagctttgg taaagtaaac attgatgttc
     3241 ccagagaccg ggaggcttct tttgaacctg agttgattcc taatagaaaa cgagatgtct
     3301 ccgacatc
//
LOCUS       DAYYAO010000159         3297 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000159 DAYYAO010000000
VERSION     DAYYAO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.166"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..856
                     /locus_tag="ACHARI_002822"
     CDS             56..856
                     /locus_tag="ACHARI_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFM8443786.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            872..1750
                     /locus_tag="ACHARI_002823"
     CDS             872..1750
                     /locus_tag="ACHARI_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFM8443787.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1763..2743
                     /locus_tag="ACHARI_002824"
     CDS             1763..2743
                     /locus_tag="ACHARI_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8443788.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3014..3106
                     /locus_tag="ACHARI_002825"
     CDS             3014..3106
                     /locus_tag="ACHARI_002825"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8443789.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT         1142 a          538 c          689 g          928 t
ORIGIN      
        1 aagaatttat agttgttacc gtagtagcta ctaaaaataa agtgaggatt aatcaatgga
       61 aacgattatt caaatcatga aaaaatatga tttttcagag atggagacaa gagtgtatac
      121 cacactatta gaaaaaggta atcttacagg atatgaagta agtaaaatct caggtgtgcc
      181 acgctcgaaa gtatataata ttttggaaaa actattaaaa aagaatttga ttgtcgttaa
      241 taaatcagaa cctaaacttt atcatgccat ctcggcaaat gagtttttag agaaattaga
      301 aaaaagtgtt aaaaatgatc tttcttttct taccaagaat ctagggatga tcaaagaaaa
      361 agatgaagaa gaaatgctgt ggaaagttga tggtatcgag tatgttctgg ataaagtaga
      421 gcatttggtg aaaaacgcaa aagaaagttt gctcattcaa gtttggcatg agaatttcac
      481 ggattcacta ctaaaggcat tgcagcaagc tgaaaaaaga gttgataagt ttgtactgat
      541 tttattcagt tctacccatg aatatgatct tcccttagaa aagtattata ttcatggttt
      601 tgaaacagac aaacttgctg actttggagc aagatggatc aacatcgtcg ccgatgagca
      661 agaagtcgtg tttggtacca ttaacgaaga actgcaatca accgatgtca cttggacaaa
      721 aaaccacgcc atggtcaatc tagcaaaaga atatgtcaaa catgatgcat atacactaaa
      781 agtgattgca gaatctcctg aagaacttaa agcaaaatat ggtgagggct ttgagggaat
      841 cagaaagatt tactagtagg aggaattttt tatgattcgg aatattttgc tgacactaat
      901 tgtcgcaatc aatctatatt ttattattta ctttctaaga gaccttttca aaaacaaaca
      961 aaatttcaaa gaagaaccag gggatatgcg tctgttgccc ttcacgtcat ttatcatctt
     1021 ctttctttca acatttggcg tttccgactt tgctatcgga accgtccttt atccaaagtt
     1081 gaaatgggtc agcatgaaaa aactacccgg aacgttgaat acccaatgtg tagtaccggt
     1141 agccgtcatg gcattgtctt acatcactgc catcaacgta gggatcaaga cactagctgt
     1201 ttgtattatc tgccaaatta tcggtgcata tttaggacca agatttgtcg tgaagttacc
     1261 agagaaaacg atcaaactgt ttgtcggcat aggattggtc attgctgcat tgttgatttt
     1321 tgcaggacaa atgaactgga ttccatcaaa cggaacagcc agtgaattgt atggcggaaa
     1381 attaatcttg gcaggatttt tactctttgt ctatggtgca ttgaacaata tcggcattgg
     1441 ttcttatgcg ttaacgatgg taactgtcta cttgctaggc ttgaatccag ttgcagcctt
     1501 tcctattatg atgggggctt gtaccttctc agtaccgatc ggcagtgttc aattcattaa
     1561 atttaatgaa tacagcagaa aaatcacact ttttacttca accttcggtg tattgggggt
     1621 attggtcgct gtattcctag tcaaatcttt agatacctat atcctgaaat ggatcgtgat
     1681 tttggtctta ctatacagtg catatacgat gttgggcagt caattaaaaa aagttacagc
     1741 agctaattaa ggagcgattc aaatgttatt tcttaaaaaa gaagatatta tcaaaagttt
     1801 ttcaatgaga gaagccatcg atgcggacaa aaaagcactg tcattgtatt cagcaggaaa
     1861 ggcaagtgtg cctcttcgca ccaatatcga tgtgccaaaa agcaacggac aaagcttata
     1921 tatgcctgct tatgtagaag gtggcgaagg cgcgctaggt gtcaaaatcg tttccgtgta
     1981 tcctgaaaac atcaagaaaa acttaccaag tgtaccagca acgatgatcg tattggatcc
     2041 agaaacgggt atggtatccg cttgtttgga tggtacttat ttgacacaat taagaacagg
     2101 cgctgttcaa ggagcagcaa cagaacttct tgcaagagaa gatgctaaaa ttggcgcgct
     2161 gattggaacc ggcggacaag cacagtcaca attagaagca atgttgaccg ttcgtaaatt
     2221 agaagaagtt cggatttttg atattgattt tgagcgtgcc agccaatttg ctgaaaaaat
     2281 gatgcaacaa ttttctgtaa cgatgcgccc aacaaaaacc agtcaagaat gcgttgaagg
     2341 agcagacatc attacaagtg tgaccacttc aaaacgtgcg acattctctg ctgagtgggt
     2401 caaaaaaggt gcgcacatca atggagtcgg tgcttacaca ccggaaatgt gtgaaattcc
     2461 acgagaaata atcaaagccg cagatgttgt tatttttgat accatggacg gtgttctaaa
     2521 agaagctggg gacttcattt caccgctgca agatgggtat attcaaagag acagctatca
     2581 tggggaacta ggccaactga tcaatgaaga attgatcggt cggacaagtg gagaacaaat
     2641 cacgatcttc aaaacagttg gttcggctgt attagatgtg gtagttgcga cagaaatcgt
     2701 taagaaagct aaagaaaata atttgggaaa attgattttc taattaaaaa ggctgaaatt
     2761 aaaaccttta ttattgcgtt taaaaaaaga aaacaaagaa gtttgtaatc aaatggaaac
     2821 tgaccccata atttaaataa aataaaaaac atctcttgtt gaattctagt aatgaagcga
     2881 ccctaaaact tagaccaaaa aatctaactt ttgggtggag ttgtcaagaa aaaccgggca
     2941 cttagttaaa aaataagttg gacgggttat actaaaattg acaccaaatt aagagaaagc
     3001 gaagatctgg attatgccaa gacgttcatt tgatgaaata tttaaaatta ccgctgtaaa
     3061 actggtaacg gaagaagggt tttctgttaa agaagttagt ctatagctag aagctcgctc
     3121 taatagcgtg tattgatgag ttcaagaagt tgaaaaatat ggataacgta cttttctcgg
     3181 tagaggaagt catgaaatcc attcgcagaa atgaaattac acgcttagaa aaagagaata
     3241 aacggttaaa agaataggta gaactcctaa aaattaccga agtcttcctg aaagaaa
//
LOCUS       DAYYAO010000160         3273 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000160 DAYYAO010000000
VERSION     DAYYAO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.167"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACHARI_002826"
     CDS             complement(<1..2177)
                     /locus_tag="ACHARI_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8443790.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACHARI_002827"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACHARI_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          902 a          730 c          527 g         1114 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gca
//
LOCUS       DAYYAO010000161         3221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000161 DAYYAO010000000
VERSION     DAYYAO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.168"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHARI_002828"
     CDS             301..585
                     /locus_tag="ACHARI_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443791.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHARI_002829"
     CDS             588..1004
                     /locus_tag="ACHARI_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443792.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHARI_002830"
     CDS             complement(1141..1860)
                     /locus_tag="ACHARI_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8443793.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACHARI_002831"
     CDS             2544..2630
                     /locus_tag="ACHARI_002831"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8443794.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACHARI_002832"
     CDS             complement(2932..>3221)
                     /locus_tag="ACHARI_002832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443795.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAYYAO010000162         3049 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000162 DAYYAO010000000
VERSION     DAYYAO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.169"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1538)
                     /locus_tag="ACHARI_002833"
     CDS             complement(228..1538)
                     /locus_tag="ACHARI_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8443796.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1550..2968)
                     /locus_tag="ACHARI_002834"
     CDS             complement(1550..2968)
                     /locus_tag="ACHARI_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8443797.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          947 a          626 c          476 g         1000 t
ORIGIN      
        1 ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct tcgcaagtgg
       61 ttctttttaa cgacttagtt atcttatcac gtctaaaaac atgtgtcaac tcttttttgg
      121 aattttcata aaatgtttca atgaagcgcg acaacgttgt ttaatatatc attaaaaaaa
      181 ataaaggtca agctaaaata ttatttaagc ttgaccttat tataatttca acgattgtca
      241 aaagtattgt ttgcagctgt gttgccagtt tctatagata aatcacagct tttgaaacat
      301 aagttttgta ctgcttcgat tcgcccgaat tcttttgcag ttatttggca atgagaaaag
      361 cagacatctt ctataggctt ttcaggaaat gctactaaat caaaagctcg ggaaggaagt
      421 gagtaacctg gagaaaggca tgcctgtacg tttttgatgt gtatgtcttt gactttcgtc
      481 atttgctttg attcaggtat ttcttcagcc actgcttgcc aactttctgg catttgagca
      541 aggtcattgc tattttttct attgtattca ttatgccagt ccattatcca agaaaaagga
      601 aattgtacat caatcatatt cagattttct accctgatat tttcgatcac tccccctctt
      661 tcttttgaag atttcattcg aataccacag tctgactgaa aaaaatcgat atcgtgaata
      721 tatacgtcac taactccagc actaacttcg ctaccaatag ttacaccgta tcctgaacgg
      781 attttacatc tagcaacctc gattcttgat gattttcgat tgactcttgc accattgcca
      841 tctctgccag acttaatagc aatacaatca tcaccacagg ccaaatcaca ttcatagaca
      901 cgcacatttg ttgaagaatc aatatcaatc ccatctgtac ttggtccatc attgttgcgg
      961 atgattactt tttctactaa tacatcgtta gaataagtga tttgcaagtt ccaaaaacct
     1021 gatttctgaa aaatcaaatc acgaacaaca acatgttctg catgatacag taaacaagcg
     1081 cgtggacgtt taatagcata atccgcaatc caacgaagtc ccttttgatc gtattcagct
     1141 cttgtcccgc ttttttgatc ttttccccaa tacagctccc accaatggtc tccttgaccg
     1201 tcgattattc cttttccttc tataagaata tctttcgccg agagaacatt caaaatcgct
     1261 gctggccatt tcatttctac gccggctact cgtgtatcga tttctggaaa tgcttcgata
     1321 tctgttctcc ccttgaggac tgcaccttct tcaaagataa gatgactctt atcttttaaa
     1381 aaaagggctc cgactaaata aatgcctttt ggaataaata caggtaagtg ttctttcgca
     1441 ccttgatcga ttgctttttg cagtattatt gtatcatctt gttgagtgtc gtttgtttta
     1501 aagttcaaag cattgataat cccttttttt gtccgcaata cgtttctccc tatctttcat
     1561 tgattttttt caattcttca ccaataaata cagtttttga acgattcagt gggtaaccta
     1621 ctagcaaaat gattgcggat acaaataagc agaatgctgg taacaagttt gccatcgtat
     1681 aaatcctttc ttgtactaaa actgattgga tggctccacc tgttgtagac gattggtaac
     1741 caatgagtgc cagcataaat ccgcctaccg ctcctgcaca tgcttgtcct acttttctcg
     1801 caaaagaatt tactccatat actgttccat cttctcttaa gcctgtctta tattgatgat
     1861 aatcaatcac gtcagtaatg aatgcccaga ccatcaaatt gaataaacca gatcctaaag
     1921 tagcgataaa taaaagaatc agatataccc atgcatttgt tatatgaagg aaatacatca
     1981 gcaagtacat acatgaagaa gcaagtaacg caaatacgct tgcttccttt ttaccaaata
     2041 attttgtcag ctttgtagca aatggtgcta aaactactac tgttccgtaa gtaaataaaa
     2101 gagcaacaga cattgcttgt ttattatgga agtaatcatt gaataaataa gtcatattcg
     2161 tcccagctaa aatctgattg atcacaatga aaatgtcaac aatgactaaa ataatcagtg
     2221 ctcgattagc taacaaacct tttaccaact gagaagcaga tactttttcg tttttgacaa
     2281 ctcttactct ttcagtagtc aatttacaag ttaacatata tgcgacaaat gctattgcag
     2341 cacatccgat tgaaatccaa aagaaatgat tccctgaaac gacttgttgt ccactcttgt
     2401 tatgggtata cattaaaatc ggaatgaaga atcctgtagt tgatccgcca atcgcagaac
     2461 caatgcttcg gaacgtggac aacgaggtgc ggtcattagg atttgagcta attgctgctg
     2521 ccattgctcc atacgggata ttgattgttg agagaaaaat cccataaaca atgtatgaaa
     2581 caaaaatata agcaatcttc accgtcattg gaagatgttg gacgaatggc aaaaacaaaa
     2641 tgacagtcac taagcaaaat ggatacttca tttttctgat ccaaggaata aatcggccag
     2701 tttctgttaa ctctgagaca tcacataatc ttccaaccat cacatcagca aacgcatcca
     2761 aaattctcga aatcaagaat aacgtaccta cgattgcccc tgaaacgcca agaacattcg
     2821 tataataaat catcaagaaa ctattgacta aacctaaaat aaaacagtta cctagatcac
     2881 cgaacatgta gcctatctta tcggaaatcc caaattcctt tttttctttt tttattgtat
     2941 ttgctgtaac agcattttgt gaaagcattt gaattcctcc tacctcttct agtaaacgtt
     3001 ttcaaaatta taactctttt tttcttgttt ttatattcac aatgttggc
//
LOCUS       DAYYAO010000163         2939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000163 DAYYAO010000000
VERSION     DAYYAO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.170"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..490
                     /locus_tag="ACHARI_002835"
     CDS             2..490
                     /locus_tag="ACHARI_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443798.1"
                     /translation="MYEKIVPRGACLLLALCLSLSAVGCQKSNENNKIKEGQSISSEE
                     EGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSDKFRNYIADKKIAMLDDQS
                     PIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKNL
                     KL"
     gene            646..1097
                     /locus_tag="ACHARI_002836"
                     /pseudo
     CDS             646..1097
                     /locus_tag="ACHARI_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            complement(1127..2289)
                     /locus_tag="ACHARI_002837"
     CDS             complement(join(1127..2013,2013..2289))
                     /locus_tag="ACHARI_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM8443799.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCAGIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVRKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            2359..2520
                     /locus_tag="ACHARI_002838"
     CDS             2359..2520
                     /locus_tag="ACHARI_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8443800.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            2539..>2939
                     /locus_tag="ACHARI_002839"
     CDS             2539..>2939
                     /locus_tag="ACHARI_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8443801.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSG"
BASE COUNT         1020 a          450 c          562 g          907 t
ORIGIN      
        1 aatttatgaa aaaatagtac caagaggagc atgcctatta cttgcattat gtttgagctt
       61 aagcgcagtt gggtgtcaaa aaagtaatga aaacaataaa ataaaagaag gacaatccat
      121 aagttctgaa gaggaaggca tgtcaattaa tgcaaatggc aaaaatgaaa cttttaagcc
      181 atcagattat acattggagg caaagaaaga atatgtttat gaatatttag ggcttaaatt
      241 taaactctca gacaaattta gaaattatat agctgataaa aagatagcga tgttagatga
      301 tcaaagtcct atagataaag aactaaagta tgctattttg acatttgaga aaatgacaga
      361 agaacaaaag aatgcagtta tagaaaagat gggcgatgga tataaaaatt ggcaaaatga
      421 gcttgaaaga attgggacta ttggtatatt tgaaaaaaat acatcagaag aaaaaaatct
      481 aaaattataa aatgtgatac tcatactaaa ataggagttt caagtgatgg aaaatacgat
      541 tgctatttaa gcacaaatag cggagctgaa agtaatcttc tggatgaatt aaaaagaact
      601 gagattcaaa ttatagataa aaaagaacgt cctaaaaatg gttttgtgct atcagaaaaa
      661 actgatttag aaaatacaga agcatttaac aaagaatcgg ttaaagattt acgtaaacta
      721 ccaacaaagg acataaatgg caaggatttt acaagtaaag attttgagaa gtatgatctt
      781 actatggtaa atgtatttgc aacttggtgt acagcttgtg taaaggaaat tcctgatttg
      841 gttgaggttc agaatgaaat gaaaagcaag ggtgtaaata tcgttggagt tgttacagat
      901 cctgttgatg ataacggtga aaacaaggaa gcaattgaaa agtcaaagtt aatacatgaa
      961 aagacaaagg cttcatatcc tttcttaatg ccagatgtgg aatggcaaca ctttttttga
     1021 caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc cttgacaatg
     1081 agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac gctgcctctc
     1141 cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc tgttgtcgaa
     1201 tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc caccaatgca
     1261 cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat tgatgcacga
     1321 attctacttt gacagattta taggttgatt ccacaacggc attatcatac ggacaaccct
     1381 tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg gtttggttat
     1441 caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa tacggtatcc
     1501 gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa taaccaataa
     1561 tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc ctcacgcgaa
     1621 cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag gtccgatcta
     1681 atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa tgagcgatcg
     1741 tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggcgc cgactgagtt
     1801 gaagcccacg ctttgctaaa cacttcttca attttcgggt accgtaagcc tttcggtttc
     1861 gaataaactc ttcctgaacg atctcttcta agtcggactc attttcgatc ggtttcgctt
     1921 gataatagta ggtttgacga gaaatattta gaattttgca catcgctgat atggaatatt
     1981 tatgcttgtt ggcatcaatt acttgtcttt tcgtccgaat atcagcgccg cttgctttaa
     2041 aatatcattc tccatttcga gttgcttatt tttctttctt agtgcgatca attctgcttg
     2101 ttctggtgtt aagttgtctc tttctttgaa tgagcccgtt gattgtgctt gcttcatcca
     2161 tttatcgaaa gaagaaggcg taagctcata ctctcgaata attcctgcgc aaggcttacc
     2221 agcgagatag agatcgacga tttgttgttt gaattctttt gaataggttc ttcgttgacg
     2281 tcttggcatg aaaaatcctc cagtgtgttt tttattattc tacacacctt attttttctg
     2341 tctagtctag tgtagccgat tcaggataag gataagtgta ttaattgtgg aaaatgtaaa
     2401 aaagtttgcc aaatgaataa agattcagtt gagaattgca atcatctgga gtgtatcaga
     2461 tgtggacgat gtaaaaatgc ttgtccagtg gatgcaatta gttatggagt aagaaaatag
     2521 aaaggagtag ttaggaaagt gataaaaaat gctatagcat atattacaag aaagaaaaat
     2581 agaactttaa taatttttat catattaact atagttcttt cttgcttgta ttcttgttta
     2641 acaataatga aatcaagcaa tgaaatagaa aaggctttat atgaaagttc taattcttca
     2701 atatcaataa caaaaaaaga tggtaaatat tttaatgtta atcaatttaa agatattgaa
     2761 aaattaaaag aaattgaaga aaaaataatt caatatgatg gattagcaaa actaaaagat
     2821 gctaaagtag ttagcggaga acaaagaata aatagagaag atttatctga cgaatttaag
     2881 aatgttgttt cactcgaagc tacaaataat actaaaagaa atattttatt tagtagtgg
//
LOCUS       DAYYAO010000164         2894 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000164 DAYYAO010000000
VERSION     DAYYAO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.171"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            573..>2894
                     /gene="esp"
                     /locus_tag="ACHARI_002840"
     CDS             573..>2894
                     /gene="esp"
                     /locus_tag="ACHARI_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8443802.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
BASE COUNT         1050 a          437 c          564 g          843 t
ORIGIN      
        1 ttataaagaa aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg
       61 ctaaaaacga gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca
      121 ttcaggaatg ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga
      181 agaaggaatt tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata
      241 aggctcctta ttccgctatt tgaatactct gaagtttaat ctaagaaaga gtaatatttt
      301 gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt
      361 tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag
      421 aaatgtactt tttttgttgt aatgtgcatg aaattcttta agtaaactag ttaacataag
      481 tttagtaaga ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactaa
      541 gaaaataact attaagggag ttgatttgaa taatggttag caagaataat aagagagtat
      601 ttctagaaaa aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg
      661 ttgcatctgt tttggttggt gttggtcttg tcttgggaac aactgaacta gttcaagcac
      721 aagatgaaat ttctccaagc accccacttg aaacagcgat aagctccgta caagtaggtg
      781 acaaagtagc ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact
      841 tttcggattt acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa
      901 ttggatttga agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc
      961 gtcttcaatt agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga
     1021 aaggaattgg atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta
     1081 caaatatttg gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggctgaaa
     1141 caactgatac acaaactgct cctaatgggg taattacgtt tgaaaagagc ttagatgaaa
     1201 tttttaaaga gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact
     1261 tggtaagtca tcaagacgat gataaaattg taccaggtat tgatagtacg ggctactttt
     1321 taacagattc agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt
     1381 ttaaacatgg atcagtaagt gcaaaatatg aggaacctaa tactcaaaca aaagatggtt
     1441 caggatccac aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata
     1501 atttctcata ttcagcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg
     1561 aacgattagt tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa
     1621 catatgatgt ttcttttgaa tcagggaaaa aagtggctga tttagcaatt gaacggcgca
     1681 aagatcatga aaattatggt gtaggttcaa ttactgataa tgatcttacc aaacttattg
     1741 attttgctaa tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc
     1801 cattagatga aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat
     1861 ttggtgaaga tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga
     1921 acacttatgg aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg
     1981 ataaagaaga gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat
     2041 atgatgttga cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc
     2101 acaaagccca acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa
     2161 atgaaaaaga tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt
     2221 caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata
     2281 aaccagaaga aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg
     2341 tggtagttgg atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg
     2401 aagatgcaga gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga
     2461 aaggtattgt aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg
     2521 taaaagcttc tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag
     2581 aaacgacagt tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag
     2641 atgccttttc aattttagat gtctataata aggacttcgg taatgcaagt gttgatgcaa
     2701 atactggtat tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg
     2761 gaacaattcc tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag
     2821 taactgtaag taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca
     2881 aagtgacctt cacc
//
LOCUS       DAYYAO010000165         2879 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000165 DAYYAO010000000
VERSION     DAYYAO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.172"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..995)
                     /locus_tag="ACHARI_002841"
     CDS             complement(120..995)
                     /locus_tag="ACHARI_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8443803.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1250..1334)
                     /locus_tag="ACHARI_002842"
     tRNA            complement(1250..1334)
                     /locus_tag="ACHARI_002842"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1298..1300),aa:Leu,seq:caa)
     gene            complement(1352..1423)
                     /locus_tag="ACHARI_002843"
     tRNA            complement(1352..1423)
                     /locus_tag="ACHARI_002843"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1389..1391),aa:Gln,seq:ttg)
     gene            complement(1433..1505)
                     /locus_tag="ACHARI_002844"
     tRNA            complement(1433..1505)
                     /locus_tag="ACHARI_002844"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1470..1472),aa:His,seq:gtg)
     gene            complement(1567..1651)
                     /locus_tag="ACHARI_002845"
     tRNA            complement(1567..1651)
                     /locus_tag="ACHARI_002845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1615..1617),aa:Leu,seq:caa)
     gene            complement(1672..1742)
                     /locus_tag="ACHARI_002846"
     tRNA            complement(1672..1742)
                     /locus_tag="ACHARI_002846"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Cys,seq:gca)
     gene            complement(1788..1859)
                     /locus_tag="ACHARI_002847"
     tRNA            complement(1788..1859)
                     /locus_tag="ACHARI_002847"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1825..1827),aa:Gln,seq:ttg)
     gene            complement(1871..1943)
                     /locus_tag="ACHARI_002848"
     tRNA            complement(1871..1943)
                     /locus_tag="ACHARI_002848"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:His,seq:gtg)
     gene            complement(1946..2019)
                     /locus_tag="ACHARI_002849"
     tRNA            complement(1946..2019)
                     /locus_tag="ACHARI_002849"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1985..1987),aa:Trp,seq:cca)
     gene            complement(2026..2106)
                     /locus_tag="ACHARI_002850"
     tRNA            complement(2026..2106)
                     /locus_tag="ACHARI_002850"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2070..2072),aa:Tyr,seq:gta)
     gene            complement(2108..2183)
                     /locus_tag="ACHARI_002851"
     tRNA            complement(2108..2183)
                     /locus_tag="ACHARI_002851"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2148..2150),aa:Phe,seq:gaa)
     gene            complement(2219..2291)
                     /locus_tag="ACHARI_002852"
     tRNA            complement(2219..2291)
                     /locus_tag="ACHARI_002852"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2256..2258),aa:Asp,seq:gtc)
     gene            complement(2301..2373)
                     /locus_tag="ACHARI_002853"
     tRNA            complement(2301..2373)
                     /locus_tag="ACHARI_002853"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2338..2340),aa:Val,seq:tac)
     gene            complement(2389..2460)
                     /locus_tag="ACHARI_002854"
     tRNA            complement(2389..2460)
                     /locus_tag="ACHARI_002854"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2425..2427),aa:Glu,seq:ttc)
     gene            complement(2475..2564)
                     /locus_tag="ACHARI_002855"
     tRNA            complement(2475..2564)
                     /locus_tag="ACHARI_002855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2528..2530),aa:Ser,seq:gga)
     gene            complement(2604..2677)
                     /locus_tag="ACHARI_002856"
     tRNA            complement(2604..2677)
                     /locus_tag="ACHARI_002856"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2641..2643),aa:Asn,seq:gtt)
     gene            complement(2687..2802)
                     /gene="rrf"
                     /locus_tag="ACHARI_002857"
     rRNA            complement(2687..2802)
                     /gene="rrf"
                     /locus_tag="ACHARI_002857"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          846 a          668 c          539 g          826 t
ORIGIN      
        1 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
       61 atcatagtcc atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt
      121 taatagattc ttgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt
      181 tgaaattgct gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt
      241 ttaaatgttt cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag
      301 cgtttaacca ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca
      361 taaacgtttg aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt
      421 tttaagctct ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg
      481 aatcctactt catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact
      541 tctactgcta tatcatattc atttggataa aggaatctca tttctggcaa cataattgct
      601 gtgacatctc tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta
      661 atatagatat ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct
      721 tccgcaaaat ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc
      781 ttttctttag aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc
      841 actttagcag aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg
      901 atcaatacag cattatgatt cagctttttc atcacataca ccgttaattc acctcctcta
      961 tatgataaaa caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt
     1021 ttaaagacaa aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt
     1081 agcaccctac ataatttaca aaaatagttt aacacataat aaaagcgtta tcaatatctt
     1141 ttttactcgt tttatgataa tacttgtatc gaattgattt ggaggtaatt acataaaaac
     1201 aaaaaaaata tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc
     1261 ggggtcgaac cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat
     1321 tccgccacgc cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat
     1381 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa agggcgaatg
     1441 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
     1501 ttcgccatca taaaagatgt tatttaattt ctcaataaaa aactaagatg aagacatctt
     1561 agtcaatacc ggcggccggg gtcgaaccgg cacgccctca acgggcactg gattttgagt
     1621 ccagcgcgtc tgccaattcc gccacgccgg caagttgatt tattagtact aaggcggtaa
     1681 ccggatttga accggtgatg aaggttttgc agacctctgc cttaccactt ggctataccg
     1741 ccattatact attatcaatc tataaaataa agaataaatt atagaaactg gggtagctgg
     1801 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     1861 gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc
     1921 gttaaccact tcgccacatt cgccatggca ggggcagcag gaattgaacc cacactaacg
     1981 gttttggaga ccgctgttct acctttaaac tatgccccta taaaatggag gagagtggat
     2041 tcgaaccact gaaccctaag gaacggattt acagtccgtc gcgtttagcc acttcgctac
     2101 tcctccatgg tggcgcggga cagaatcgaa ctgccgacac acggagcttc aatccgttgc
     2161 tctaccaact gagctaccgc gccatatttt aaaattatta attgaataac aagaacgacg
     2221 gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac
     2281 accacggaac cagttcatta tggaggttaa cgggatcgaa ccgctgaccc tctgcttgta
     2341 aggcagatgc tctcccagct gagctaaacc tccaacattc aattaaaatg acccgtacgg
     2401 gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc
     2461 aggtaaattt gctacggaga gtaagggatt cgaacccttg agacagtttg gcaccatcta
     2521 catgatttcc aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc
     2581 aatactcttt ctcaaaatga gaactccggc agtaggactc gaacctacga catcatgatt
     2641 aacagtcatg cgctactacc aactgagcta tgccggaata acatcagcgt ggcgacgtcc
     2701 tactctcaca aggggcaacc cctcactaca atcggcgcta agaagcttaa cttctgtgtt
     2761 cggcatggtt acaggtgtat ccttctcgct atcgccacca cactgtggtg ttatctttta
     2821 ttgagtaaat tttgttcact caaaactgga tttgaagtca cataagtttt tttccgagt
//
LOCUS       DAYYAO010000166         2796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000166 DAYYAO010000000
VERSION     DAYYAO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.173"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHARI_002858"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHARI_002858"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8443804.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1085 a          435 c          569 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DAYYAO010000167         2741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000167 DAYYAO010000000
VERSION     DAYYAO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.175"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..523
                     /locus_tag="ACHARI_002859"
     CDS             <1..523
                     /locus_tag="ACHARI_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443805.1"
                     /translation="YLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTK
                     GMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITL
                     PVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTA
                     YLCLFFKIVFHKR"
     gene            698..1531
                     /gene="msrA"
                     /locus_tag="ACHARI_002860"
     CDS             698..1531
                     /gene="msrA"
                     /locus_tag="ACHARI_002860"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8443806.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1611..2600
                     /locus_tag="ACHARI_002861"
     CDS             1611..2600
                     /locus_tag="ACHARI_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8443807.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1001 a          434 c          529 g          777 t
ORIGIN      
        1 ttatttattt atgactgttt tgttattaat cactttaact gtttctagta aaaaaaattt
       61 caatcaaaaa ataatttctc agccgggaca tattgttgta aacaaagaaa cagtagaggc
      121 gtataccact aaaggaatgg attatttttt atatgattat tctcctatcg cttcattgga
      181 gaataaggag catataataa aacacgaagc aaagattggc agtcaatggg taaaccttcc
      241 ttataaaaca actggtagtt ctatcatata tagagtaact aatgagaaga gccaagtaat
      301 cacacttcct gtatatgctt atttaggaac gaatgtagaa ataaatcatc aacctgtaaa
      361 ggagcaggtg aatgctaatg gattggtaca aataagcgga gtcaaaggag ttgctgatat
      421 taaagtaagc acaagttata cagtaacgac tattatttct tatcttgtta cattaactgc
      481 atatttatgt ttatttttta aaatagtttt ccataaaagg taaattaact tttcatttat
      541 aaaataaatt gattaaaaag gagaatctgt cgataaggat tctctctttt ttatacgaaa
      601 aatctgtagg gggatgaagt aatcgcttat ttcctcactt tatgataaat tacagctaaa
      661 gtcactagct tgtcttcttc ggaaaggagg agaatctatg gatgaggaac aaatattcat
      721 tcaaacactt tataatctga ttttgaatcc gaatacacga gattgggaac gaaaagtatt
      781 gattcagaca aagaatgaca taagagaaaa tatatccgtt aaagaacagt tatcaaaact
      841 agaagcaaca ctacgtccat tagctatccg aatgaatctg acgcctgatg taatggactt
      901 ttatttattg attacggaag gatttgataa ggagcaaaaa tatgactttt ccaagcatgc
      961 catgcaagat gctgactatc aagaacgtgc agtatttgca gggggatgtt tctggtgtat
     1021 ggtcgagccg tttgaatcga aaaaagggat cctatctgtt ttgtcaggat acacgggcgg
     1081 acatgttgaa aaaccaaatt atgatcaagt aagtggtggt tacacaggtc atgtggaagc
     1141 tgtggagatc atttatgaca caagagaaat aagttatagc gagttgctga caatctattg
     1201 gcaaattact gatcccacag atacatttgg acaatttcaa gatagaggga agcaatatcg
     1261 tccagtgatt ttttatcaag gcgaacgaca aaaagaatta gcggaacaat caaaacagaa
     1321 actagacagt tcaggaactt tccaccaacc aatcgtcaca aaaatcgaac cagccggtac
     1381 gttttggcca gcagaaaact atcatcagca attttacaaa aaacaaccga aacgttataa
     1441 aaaaatccag caagcacgta atcaatttct gatctatcaa cgagtaaaaa acaaatggca
     1501 aaaaaatata cgaaaaaatc attttgatta actccttttc tttaaaatgt agtaaactaa
     1561 aaatagaaaa aagaaagcgt tttaaaaaga tttgatgagg aggagaaaaa ttgtatattg
     1621 cagatagtgc acgttatgaa aaaatggaat atcgtaaatc cggtcattca ggtttgaaat
     1681 taccgatttt atctctcgga ttatggcaaa attttggtga ttatgatcct atccataatc
     1741 aaagagagat tttaagaggc gcattcgaca tgggaattac ccatttcgac cttgcgaata
     1801 attatggagg ccctgctgga gctgcagaga aaaactttgg gcgtattttt agagaagatt
     1861 ttcaagccta ccgtgatgaa ctgattattt ctagcaaagc aggttatcat atgtgggaag
     1921 ggccatatgg ggaatggggc tctcgtaaat ccatcatttc aagttgtgac caaagtctcc
     1981 aaagaatggg gctagattat gttgacatct tctatcatca tcgcccagac cctgatacac
     2041 ctttggaaga aacagcagaa gctttgatgc aacttgtaag acaaggaaaa gcattatata
     2101 ttggtatatc caactataat ggagaagata caaaaaaaat gactgaaatt ttaaaaagaa
     2161 aggaagcacc gttcattatt catcagatgc gctacaatat gttttctcgt gcactactag
     2221 aagacgatct atctccagta ttagaagaag aagggctagg ggcaatcacg ttcagtccgc
     2281 ttgctcaagg gttgctgaca aatcgttatc tacatggtat tcctgaagat tctagagcac
     2341 atcgtaagga aataccattc ctttcagaag aacaagttgg atcaactctg gaaaaaatca
     2401 aagcactgca aacaatcgcc gtatcacgag gacaatcatt agcgcaaatg gcccttgcat
     2461 ggaatttgcg acaaaaatct gttacaagtg tactggtcgg agcaagtcgg ttaagtcagt
     2521 tacaagaaag tgttcgaatg atggataatt tagatttctc accagaagaa gaattgagaa
     2581 tcgaccaaat tctagaatag aaacgatgta atcaaacttt ttatccatat attaaaaaaa
     2641 acaaaagatg acatcaagaa tcgaatgaag atcgtctgtt attgaacatc atattttttg
     2701 tcgttcaaaa taaaaatgat cgaaggctag gagtgaattt t
//
LOCUS       DAYYAO010000168         2680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000168 DAYYAO010000000
VERSION     DAYYAO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.176"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1156
                     /locus_tag="ACHARI_002862"
     CDS             149..1156
                     /locus_tag="ACHARI_002862"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8443808.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1168..2172
                     /gene="neuB"
                     /locus_tag="ACHARI_002863"
     CDS             1168..2172
                     /gene="neuB"
                     /locus_tag="ACHARI_002863"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8443809.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2169..>2680
                     /locus_tag="ACHARI_002864"
     CDS             2169..>2680
                     /locus_tag="ACHARI_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8443810.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPV"
BASE COUNT          976 a          339 c          526 g          839 t
ORIGIN      
        1 aattttttct ttgatacttc atattttaat caatcaaatg ttgattcgtt acgttttgac
       61 atcattattg ataattagtt tagttatata ttttttaaag attaagaatc gaattataga
      121 tgactataat tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg
      181 cggaactggt tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc
      241 tatcagaatt tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa
      301 tcacactaat ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc
      361 atgtcgtgat gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga
      421 atataatcct atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc
      481 agcgatcaaa aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc
      541 tactaacacc atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca
      601 gtataaagga aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc
      661 aagtggatct gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt
      721 gacagatgtt gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct
      781 gtctgctgct atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa
      841 tattttaact ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata
      901 tagaggtgaa ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat
      961 gactacagac gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca
     1021 ctatcatgct actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc
     1081 ttcagatgca acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa
     1141 tgtcgtgact tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt
     1201 ggtgtaaacc ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa
     1261 tgtggagcta acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag
     1321 gctccaatgg caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg
     1381 ctaaaaaaat tagaattatc tcaatctgat tttattagaa taaaagatta ttgtatacaa
     1441 aataaaattg attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa
     1501 attggtgtac aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga
     1561 aaaatagata tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag
     1621 gtcagtgagg ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca
     1681 aattatccgg ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg
     1741 ttcgggatac ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct
     1801 gttgcgatgg gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt
     1861 ccagatcata aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga
     1921 aacactgaaa tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca
     1981 aaagaagtat caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt
     2041 acagagaaat ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat
     2101 caatatattg gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa
     2161 gatgttgaat gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata
     2221 aacctgttgc tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa
     2281 tgcatttatc ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa
     2341 ttagtggaga agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg
     2401 tggcattaga gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat
     2461 tattactagg ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa
     2521 tcaaaacagc acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac
     2581 atgccataac gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt
     2641 taataaaaat gggggaagat tcttggagag tttttcccgt
//
LOCUS       DAYYAO010000169         2524 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000169 DAYYAO010000000
VERSION     DAYYAO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.177"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(965..1483)
                     /locus_tag="ACHARI_002865"
     CDS             complement(965..1483)
                     /locus_tag="ACHARI_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8443811.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            1850..2098
                     /locus_tag="ACHARI_002866"
     CDS             1850..2098
                     /locus_tag="ACHARI_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443812.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT          822 a          458 c          362 g          882 t
ORIGIN      
        1 tttacttggt atatttatga ataatcgcaa atttacccct atactactag tttgtaagtt
       61 agattaataa aaatactaaa gttatttgtt cttttattaa atacacctac aacttataaa
      121 ttgatggtat ttagataaca cgaaagaata aaaataacag ctgatagtaa agtttgttga
      181 cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga aaacttatga
      241 aatgaaagac cttttacatc ttgcagaaat cgttactggg attaaactaa gcaataaaaa
      301 ctaatgttcc cctcattctt tgaacttcta ctatttgatt tatttattct gataaatgac
      361 gatttcacct tcaggatctt caaatcccgt atagaattta tttaagatct cttcagtatc
      421 agtaatagga taggcgatag gccacatacc ctttgatggt tagaataaac tgctcaaaat
      481 ctgtatcgta tgttggcttt agcatattaa caaaggctat ctccaaatta taaaccataa
      541 gcgttgaatc gttcttaaca atttcaattc ttcctaaccc aaagtcagaa gatataatta
      601 ccggacttgc cttacccttc atgtgggttg ttgagattcc atatttaaag ggaattgtcg
      661 gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa atctttcccc tgctagtctc
      721 ctaagtccct ttgtagcgga tatggcacca taatctcatt ttatcgaagt gagtcatggt
      781 ttatctgttg gccaaccaaa attgatacaa tctaacgatg caccctctac ggcattctag
      841 gggagtgcac ttgcattcac ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg
      901 tatgttctta ctgttaacat acaagaggtc tcctttccac aattgcttag cagtaaacgg
      961 gaatttacaa agctaatctc ttccattttc ttatctttgt tctaaaagtc ccaaatggag
     1021 caactgtatt tacatgaata aatttatata cttcccatac agccgttttg gttgcctcgt
     1081 ctgcccattt tcttttgtgt gcagtgaata gatcttcatc actcattgaa tcaatcatca
     1141 tgttaatatc atcaatatta cgattgagaa tttcctctaa ttctttcaat ggtagatgtg
     1201 cataggtatt tgtaaaccac ttatataact ctcctaactg attccattta aattgatcag
     1261 aaggcgtttt aacagataat ccatttcttt catcattttc ccattttaga attaaattcg
     1321 tccaaccaac ttgatatgct aaattttcag caggtgttct atccacttcc tcaactcttt
     1381 tatccttcaa ttcttcagga atagtttcaa attctaaaat atatttagta taagtcctat
     1441 ttatttcttt ttttaattct tctttatttt tatatgttct cattatatta cctccataaa
     1501 ttcctaattg tctcctttaa ttcatctata atttcatcca cgatatttta atccgttcta
     1561 caatgcatat ctgatatgca ttgactgtct aactcatatg tgaatgattt ataaaatatc
     1621 ccaacgtctc atctaaagaa atgttgatcg tcagcaagga gctaaaaaat acgaatatat
     1681 ttcttttgta gtaaaatcgg actggtttgc atatcatttt cttcgtaaat atcaaaatca
     1741 tgttaactac tatcaattac taataaaaac taccacttta agcgttaaga actgttaagt
     1801 ttagttaagt gaggatagtt cacaaaatcc attgttaaac ttcacgaaag tggtaaaact
     1861 caaaatgctt tatataggat tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa
     1921 atattatgaa gtaaaaattg acgatgacac gttccttacg gctcatcaga tgaaacagct
     1981 acaaaaacga aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat tgccatattc
     2041 acgcctcgct cgacgaacta ttaaaggtca agatttacct gaagatcatc ctttttagga
     2101 aattgaagct ttttatgttg cacaatgcat ttataatact acaatgatgt tttcaccaga
     2161 tattcttcta gggtgcggtg taatgaaaca gatacacttg caaaagaaaa ttcaccgcga
     2221 atttactaaa attatgaata attatgttaa tcttccgaaa tttttttcca aaattaaggg
     2281 atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat
     2341 tatagaaaac cgatactctg ttaagttggt tgacacggtg tcgattttta tgactttctt
     2401 attctgaaac ttcactaacg gacagtacct aaaaagtcta tagtatggac gtttacgagt
     2461 actattttat caaaaatgct gctcatttca acttatatgt tttagtgaac tacccccact
     2521 tagc
//
LOCUS       DAYYAO010000170         2490 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000170 DAYYAO010000000
VERSION     DAYYAO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.178"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACHARI_002867"
     CDS             complement(365..1657)
                     /locus_tag="ACHARI_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8443813.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACHARI_002868"
     CDS             complement(1931..2191)
                     /locus_tag="ACHARI_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443814.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          844 a          465 c          381 g          800 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
     2341 gtaaaatatt ttgtgtaaat gaaaaaatcc atacaaaaaa ggaaggcgct tctgtagaat
     2401 aaagttaacg acaaccaatt cacagaaaag aggacttccc tatgaatgat tttactacag
     2461 aaattgtgca aactctagtc actaaaggcg
//
LOCUS       DAYYAO010000171         2425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000171 DAYYAO010000000
VERSION     DAYYAO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.179"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACHARI_002869"
     CDS             <1..1368
                     /locus_tag="ACHARI_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443815.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..>2425
                     /locus_tag="ACHARI_002870"
     CDS             1355..>2425
                     /locus_tag="ACHARI_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8443816.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMI"
BASE COUNT          803 a          368 c          417 g          837 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgata
//
LOCUS       DAYYAO010000172         2400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000172 DAYYAO010000000
VERSION     DAYYAO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.180"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHARI_002871"
     CDS             207..2069
                     /locus_tag="ACHARI_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8443817.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACHARI_002872"
     CDS             2069..>2400
                     /locus_tag="ACHARI_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8443818.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAYYAO010000173         2389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000173 DAYYAO010000000
VERSION     DAYYAO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.181"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..249)
                     /locus_tag="ACHARI_002873"
     CDS             complement(4..249)
                     /locus_tag="ACHARI_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443819.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ACHARI_002874"
     CDS             complement(266..1117)
                     /locus_tag="ACHARI_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8443820.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ACHARI_002875"
     CDS             1283..2287
                     /locus_tag="ACHARI_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8443821.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          763 a          403 c          483 g          740 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgag
//
LOCUS       DAYYAO010000174         2325 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000174 DAYYAO010000000
VERSION     DAYYAO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.182"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..2175
                     /locus_tag="ACHARI_002876"
     CDS             22..2175
                     /locus_tag="ACHARI_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8443822.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          799 a          414 c          519 g          593 t
ORIGIN      
        1 aaataggcgg aaggatagga aatgatgaaa gattttatga aaaaaatatt aaatacttct
       61 tggttagaaa aaataagaaa aggtgcacaa gaacctaaga acaaaacgtt tcgccggtcc
      121 catgttcctt ttcgcttaaa ttttcttttt ttcattatat ttacgttatt tgttgcgttg
      181 attgctagat tgggttattt gcagatcgtg aacggcgaag agatggaggc gcgagtgaag
      241 tctacttcta cgattacggt ccaagaaagt actcctcgcg gaatgatcta cgactccaca
      301 ggaaaagctt tagttacgaa taaagcgaat caggccatca catatacgag aggaacacaa
      361 gttacggcta aagatttgct tgaaatcgca acgaagctga actcgctgat cgatgttccg
      421 gtagatgaaa atctgacaga tagagataaa aaagattttt ggctggctga tcccaaacat
      481 ttgaaagaag ccacaaaaag actttcggca aaagaaaagc aattagggaa cagcgaacaa
      541 tatagtgcga tggtcaataa agtcacagct gatgagatta atttaaacga ggagcaatta
      601 aaaattgcta ccatctttaa acggatgaat agtgcatatg aattaaacac tgtctatatc
      661 aaaaactctg gtgttactga tcaagagtta gctgttgtgg cagaacgcgc ttctgaactt
      721 ccaggagttt cgactggcac agactggaca cgagaatata atgcagcaag ttcactgaaa
      781 agtattcttg gcagtgtgac gactgaaaag caaggacttc cagcagatga agcagagaaa
      841 tacctagcaa aaggttattc tcggaatgac cgtgtaggac aaagttattt ggaaaaacaa
      901 tatgaagatg tcctgcaagg aaccaaaacc caatatgaag tttcccttga taatgaagga
      961 aatgtttcga atcaaaaaga aatcttctct ggggaaaaag gttctaattt aatgttatct
     1021 atgaatgcag aattccaatc aaaagtagaa gaaattttga aaaggaatta tcaaacattg
     1081 atcaacaacg gcaaagcaca gtattcgcct ggtgcttatg cagtggctat gaatcctcag
     1141 acaggagaag tgctagcaat gactggtttc agtcatgaac aaggttccaa agaaatcacc
     1201 gagaatgctt taggcacgat tactagcgcc tttgatccag ggtctgtagt caaggctggt
     1261 actttgacag caggctgggc aagtaacgcg atcagcggaa atcaagtttt gattgatgag
     1321 ccgattcgtc tgcaaggtgc ttcagaaaaa agctctgtct ttaatcgaag cggacaagtt
     1381 gctcttgatg cagtcaaagc atcggaattg tcttctaata cgtatatgat caaagtggcg
     1441 ttaaagatgt taggactaga ctatacacct ggaatgggac tacctagttt agatgaagaa
     1501 gctaaagctt atcagcaatt gagagactct ttcaaagagt ttggtcttgg tacaacgact
     1561 gggatcgatt tgccgaatga atctcctggg atctcacgtt ctgttgatta tatgaagaag
     1621 ttcaatgcgg ataatggcaa agaatggtat gctccaggta actttaccga tttggcattt
     1681 ggacagtttg acacatatac accaatccaa ttagctcaat atgcttcaac agtcgctaat
     1741 ggcgggaaac gagtacagcc gcacctagtc aaagcgatct atggcaatga cgaaaatgga
     1801 aacttgggtg aagtgaagaa agaaattggc acaacagtag aaaatacggt caatatttct
     1861 gcagaaaata tggcgatcct ccgtgaagga ttccaccaag ttgtccatgg taccgatcct
     1921 tacacaacag ccaaaccatt agccagtgcg aaaatggacc tttccgcaaa aacagggact
     1981 gcagaaacgg ttgcggaagg tcatccagat attgcgacag tcaatagtaa tatcgtggca
     2041 tacggaccaa cagacaatcc ggaaattgcc atcagtgttg tcttgccgaa cttattagac
     2101 gaacaagacc acataaactt gacgattgct aaagaaataa tggacgcgta ttatgatatg
     2161 ttcatggcaa aataaaaaag aggtcgtgaa aaagccgctt agtttcgagt atgaagaaaa
     2221 attttgtgaa aatagcttct cacatttttg cttaaatttg acttcatacc gagaaactgg
     2281 cttttgaaca ccgtttactc ggcaaaaaag gttgtgacaa aaatt
//
LOCUS       DAYYAO010000175         2295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000175 DAYYAO010000000
VERSION     DAYYAO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.183"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1178)
                     /gene="xerS"
                     /locus_tag="ACHARI_002877"
     CDS             complement(93..1178)
                     /gene="xerS"
                     /locus_tag="ACHARI_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8443823.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1527..1722
                     /locus_tag="ACHARI_002878"
                     /pseudo
     CDS             1527..1722
                     /locus_tag="ACHARI_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
BASE COUNT          761 a          330 c          368 g          836 t
ORIGIN      
        1 aatataaaaa tgataatgaa gcttgccttt aaagttgggc ttttatctaa ttttagagac
       61 aggcttcatt attatactat tttttactac atttacaaag cgttcaaagc atcaattgta
      121 gctgcattgt ctatatgagt atataaagtt gttgtatttg tacttgtatg tcccaattgt
      181 tgggcagtta ataccaatga atttgtctgt tgatagagtc tagtagccaa agtatgacgt
      241 aatttatgtg gactgacttg gactctgaat ccttttgaat attttgctac catccgttcg
      301 attgcaatac cggatatacg cttggcttct ccttgataaa ttgctagaaa taaagctttt
      361 tcgttttctg ttactttgta tcgttctttc cgaatagcta gataattagc taaatattcc
      421 attgcaaatg ctgctatatt tactgcgtct ctcttatttc cttttctagt tactaccaca
      481 ttattctttt ctaaagatag gtcctctaaa ttaatattta caacctcgga gagtcttaat
      541 ccagatccta aaatcaatgc attgatcgcc aggtctcttt ctacatctcg ttgataatag
      601 atcaaagctc gtttgctcaa agttttttta tattcattgg caatataatc tagataattt
      661 tgtgtatctt cgtctagaaa taattttttc tcgattgcgt ttgcacgggc agctaacgta
      721 gttttatctg tgatagttgc tacttgatcc ataggattat tttctaagta tggttttcct
      781 aaaggattat tggaactttt cgaaagatat ttaaatagaa cctttaatgc tgtgatattt
      841 ctattgatag tagaagtaga tagacctaag ttattattat ctttgagctt agtttttgtt
      901 gtctcttttt ttacattttc ctttgtgcga cgtttcaaat gagatttgta caagtttaga
      961 tcataggcag ttaactgtct tatcgaggtg attgaagtat cgggaacgat agactcactc
     1021 agaagccatt cataaaagag cttataattt tttagatatt ctaaagtagt tcgaggtgat
     1081 tgatctttat cctcacgatc ttgaacaaaa tcttttatat aatcgggcat atgaagtatg
     1141 attgtatcta ttttttgata tatttctgta tttttcatgt tattcatcct ttcgataagt
     1201 aggggagagg agtatctcta taattggact ataccaatta tagagtgttt tgtcaattgg
     1261 tatactctga aatttattaa tttatttcaa aaaagcagat aaataggtat agatttaata
     1321 atttaaaata aatggtatac atgaatagga aaggaaacag aaggaggcaa tcttaatttt
     1381 ttaaattgtc taaattatat agaaagaaca atctttctat ataattagac ataagagaga
     1441 ctacggttga tttgatatta acaagcgtat atgaatgtat atacaaataa aaaaggggag
     1501 ggagaagtta aagaaaattc agagaagtgg ggcactcagt agtattaaca atttaaaagg
     1561 aatttcttaa agaaaatggg ttgcatcctg gagatacaat ttttttagat aaagaaaaaa
     1621 ttcaagcaac aattcaaaaa aatcttaact gctgagattg atgtagcaat atctcaatca
     1681 tcaacaaagt acgatcctgc ctttcaaaag cttattgata aatgtttatt cattataaaa
     1741 ataaaagaaa aatttatgtc agcttttttc atcaaagtgg taatgataat tcgtctatta
     1801 attgtatttt ttaggaaata tggtataatt gttttaacga aaaggtaatt catgatacac
     1861 caaaaaccac tagctggcgg gctagtggtt ttcttttacg ttctattcag attaacgggt
     1921 aggcttactg tcttttttgg caataatttt gcaatcaagc catttacaga tgtaatgcgc
     1981 ggatacttct gctaagattg caaaaaacat atcgaaaagg taattcataa tacaacctcc
     2041 tccccttgcc ataataggcc ggggtagcag acagcataaa tattatacca taactgaaaa
     2101 tttcatttta gtacatttca atatattgat gaaccagcac aataaaacat gccatgacta
     2161 acagaaaaaa acttattaaa ccagatataa cttgtaaaca tccaactata tagaaaacgt
     2221 aaattttcta tatagttgga ttaagataac gactatttat tttatcatat ttttactata
     2281 ccctatactt ttact
//
LOCUS       DAYYAO010000176         2293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000176 DAYYAO010000000
VERSION     DAYYAO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.184"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACHARI_002879"
     CDS             complement(51..1598)
                     /locus_tag="ACHARI_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8443824.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHARI_002880"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHARI_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8443825.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACHARI_002881"
     CDS             complement(2043..2237)
                     /locus_tag="ACHARI_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443826.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DAYYAO010000177         2254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000177 DAYYAO010000000
VERSION     DAYYAO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.185"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..958
                     /locus_tag="ACHARI_002882"
     CDS             110..958
                     /locus_tag="ACHARI_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8443827.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            1058..1492
                     /locus_tag="ACHARI_002883"
     CDS             1058..1492
                     /locus_tag="ACHARI_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8443828.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(1642..>2254)
                     /locus_tag="ACHARI_002884"
     CDS             complement(1642..>2254)
                     /locus_tag="ACHARI_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8443829.1"
                     /translation="GEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEE
                     EFGHLEGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNL
                     FKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMDF
                     NQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          656 a          362 c          426 g          810 t
ORIGIN      
        1 cataataatt tagactagcc taattttttt attaattaaa gttaacttaa ggtgaaaagt
       61 aagttataat aagataagag tattattaat gaattaagga agtgaataaa tgaaagaaac
      121 gagacaattt atatggacat acattaagat tatttttgcc ctcttcatat taggagtcgc
      181 tatcaatatg tttttagggc ctcatcatat tgctgctgga ggtgtcagcg gtctaggaat
      241 attactggaa gctgctttag gctttgaccg agctatagtt attttagtgt tgaatatcgt
      301 tatgttggta ctagctcttc tatttttagg taaaaaaccc ttttttaaag tacttttcgg
      361 aagcttggtt tttcctttaa tcattgcaat agttccagaa acaatgatta cttcagatcg
      421 gttgttgtct gtcatttttg gaagtgcgat ttttgcttta ggagttgcga ttttatataa
      481 aaacaactct tctagcggcg gtacgacgat ccctcccctc atttttaaaa aatatttcca
      541 cttgaatact tcgattggct tattttttac agacgcattc gttgtttctc ttaatctgtt
      601 tatttttgga atagaagagt ttctttatgc tattttatct attgcaatca cttcaattgt
      661 catgacttat atcgaaacag gtatcaatcg aaagaaatct gttatggtca tgagtgaaca
      721 ttatttggaa gacatccgcc ttcgcttaag tgctgagatt ggacgtggcc ttaccctgct
      781 agaggcaaaa ggcgggtata atcgaaagtc aaaagaggtt ttattgatcg ttgtgactga
      841 tcatgaattt tcccgaatga aaccattgat agaagaaatc gatccaagtg cctttgtcat
      901 tgtcagcagc gtagcagaag taatgggacg cggatttact tatcatccaa tcgaatgagt
      961 tggtaagtaa atccgctatt tttttgaatg cgttttcttt agctggtata ataaaataat
     1021 ctagtaactg ttcgactgaa aaggagagga gatctttatg cataaaggaa cggaaatcga
     1081 ttttgtatta aatgatagtt tgaaagcact agaactgtat caaacaatct ttgatgaaat
     1141 acaactgatt gaggcaacag attaccctag aggcaataat gaagtcattt ttaccttgta
     1201 tggtacgaga ttccatatgc tggatgaaaa cacgtctctt ggtttgactg ctcctactgc
     1261 tgaacatcca aatacgatct ggttcaatat catcgtctct gatatcgagg acacacataa
     1321 taaagcatta cgaaatggct gtgtagagat tcaagaaatg acagaaatga tggactttgg
     1381 tgtgactaac tcaatgttca gagatccttt tggttattta tggatgcttc atcaggtcca
     1441 tcgtgaagta atttttgaag aaagaaaaca actttttgag aaatatatgt aatgtttatg
     1501 atgagattat tttttgtctc tgatattttt tgtatatgtt tgctattcga gaaagcgcgt
     1561 ctgtttcatg tgctttctct tatttatctt atgccaatcc atttctttgc tttccacaat
     1621 tatattgtat tatttgtcaa attaagcgag acaaaacttt ccatttacgt aactcaaaaa
     1681 aacttctaat ggtgtttggt aatttagtga ttttctaggg atatggtttc ttttggacgc
     1741 aacggatgag atgaatcctt gattgacttg gttgaagtcc atttcttttg gtagtccatc
     1801 ttttcggagg agtccgtttg aatgttcatt taatccccgt tgggaaggcg ttcctgggtc
     1861 tgcgaaataa atatcaatat cattcgtatt actaatactt ttccaattag agaattcttt
     1921 tccacaatca aaggtaattg atttgaaaag atttttgggt acggattgaa gccattcatt
     1981 aatcgaattt tcaatatcaa ctgccttacg gccttctggt ttcaaggcga taatggcttt
     2041 tgaaagtcgc tcaacgagtg tgatgacggc acttttatgg tggatgccga caatcgtatc
     2101 accctctaaa tgtccaaact cttcttcgaa aacgacataa tcttttttac gttcagaaat
     2161 atttcttttg aatgcctgtt ttccacgttt ttccttataa ccatttggtt ttcgttttcc
     2221 ttgcatcggt aaatgtaaaa cattgaattc tccc
//
LOCUS       DAYYAO010000178         2253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000178 DAYYAO010000000
VERSION     DAYYAO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.186"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            581..1540
                     /locus_tag="ACHARI_002885"
     CDS             581..1540
                     /locus_tag="ACHARI_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8443830.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1527..2132)
                     /locus_tag="ACHARI_002886"
     CDS             complement(1527..2132)
                     /locus_tag="ACHARI_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443831.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          768 a          363 c          334 g          788 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaac tttgtaaaaa
     1561 tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt
     1621 ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa
     1681 ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca
     1741 ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta
     1801 tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc aaaaattttt
     1861 tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac tgataattga
     1921 tactctatat tacatgactt aattagtttt ttagatcccc cctccagata ggcatatgtt
     1981 tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc ataaattaaa
     2041 aatgactgtc tatttagccc ataatctaat acaacttctt catactcata tttctttaaa
     2101 tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt gttatttatt
     2161 gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt agtgaactac
     2221 tcaccactta gctaaaccta acggtttctt acg
//
LOCUS       DAYYAO010000179         2211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000179 DAYYAO010000000
VERSION     DAYYAO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.187"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..>2211
                     /gene="acm"
                     /locus_tag="ACHARI_002887"
     CDS             96..>2211
                     /gene="acm"
                     /locus_tag="ACHARI_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8443832.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          868 a          343 c          440 g          560 t
ORIGIN      
        1 agattggaaa tgagtggggg aggaattgaa gaaaggattt ttgagagatg atatagtagg
       61 ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga aatattattt
      121 tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat gtctatgcgg
      181 atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca acgaatatta
      241 cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa aatatccaac
      301 caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt gaaggattcc
      361 aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg gtcagaaaag
      421 attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta gaaggttggg
      481 ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat acgggaccat
      541 tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt
      601 cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat actaagcaca
      661 ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca gttaaaatca
      721 gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt aatcaaatac
      781 acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt ttacaagatt
      841 tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata actataccaa
      901 aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata gaaaacccag
      961 aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat aaacctgctg
     1021 ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt ggagtcaacg
     1081 gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag attggtatcc
     1141 caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga cagtctagta
     1201 tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa gtaggagact
     1261 atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta agttcaaatg
     1321 aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct atttataaca
     1381 agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc actccaagac
     1441 cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg
     1501 cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat atccaacagt
     1561 atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa aacggaaatg
     1621 attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca aatgagaata
     1681 aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac caagacggca
     1741 aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc caacacaaag
     1801 aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa tacagagacg
     1861 ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt acgacgattg
     1921 acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct ataccaggta
     1981 gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa attagaaatc
     2041 tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa caaagaagca
     2101 attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa agcattcttc
     2161 ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg a
//
LOCUS       DAYYAO010000180         2189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000180 DAYYAO010000000
VERSION     DAYYAO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.188"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACHARI_002888"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACHARI_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(987..1952)
                     /locus_tag="ACHARI_002889"
     CDS             complement(987..1952)
                     /locus_tag="ACHARI_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8443833.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DAYYAO010000181         2149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000181 DAYYAO010000000
VERSION     DAYYAO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.189"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..517
                     /locus_tag="ACHARI_002890"
     CDS             <1..517
                     /locus_tag="ACHARI_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443834.1"
                     /translation="YLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAG
                     CSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCR
                     RSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFARED
                     EDNPKTYDILA"
     gene            complement(578..943)
                     /locus_tag="ACHARI_002891"
     CDS             complement(578..943)
                     /locus_tag="ACHARI_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8443835.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1019..1264)
                     /locus_tag="ACHARI_002892"
     CDS             complement(1019..1264)
                     /locus_tag="ACHARI_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8443836.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1334..1588)
                     /locus_tag="ACHARI_002893"
     CDS             complement(1334..1588)
                     /locus_tag="ACHARI_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443837.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1752..2075)
                     /locus_tag="ACHARI_002894"
     CDS             complement(1752..2075)
                     /locus_tag="ACHARI_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443838.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          809 a          363 c          374 g          603 t
ORIGIN      
        1 atatttagaa attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt
       61 ctattttcag ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct
      121 agcaatggca ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga
      181 agccacgaca gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg
      241 acgatgcatg ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag
      301 gtgtagaaga agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc
      361 atcgtataca gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat
      421 gtctagggaa gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga
      481 tgaagataac cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta
      541 atgaagtaag aggagataga aggcttttct agttatatta ggtaagataa attccttttt
      601 gctccaaatt tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca
      661 cgttgcgctc tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag
      721 gatttttcca ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct
      781 gatgatattg gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt
      841 tctttcccca gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt
      901 gacgaggaag ctttgaaatc aatttttcat gccataatcg catagaaatg tctcctttat
      961 atatagattg ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc
     1021 acttcagttt gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa
     1081 agtcagcgat atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac
     1141 gatatttgaa taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc
     1201 cagttcttaa gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac
     1261 tcatttaatc acctttcatt atagtagaat agttataatg actatagaaa gctacacatg
     1321 caatcaatag tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata
     1381 aagacaaaac cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt
     1441 aattaaaaac tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc
     1501 gttctttgtt ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc
     1561 aaaacaaaac tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa
     1621 agataacatg ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga
     1681 tcaatgaaaa atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga
     1741 atgtgtctta tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat
     1801 aaaaagagga cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa
     1861 acatatttaa caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc
     1921 atcatgacaa gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt
     1981 cctgaggttt gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa
     2041 atcgcaataa atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt
     2101 ttttctaaaa taagcaaata aaaattaaag taacaaaata gttatgaca
//
LOCUS       DAYYAO010000182         2148 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000182 DAYYAO010000000
VERSION     DAYYAO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.190"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(383..1849)
                     /locus_tag="ACHARI_002895"
     CDS             complement(383..1849)
                     /locus_tag="ACHARI_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8443839.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          680 a          424 c          350 g          694 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtc
//
LOCUS       DAYYAO010000183         2056 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000183 DAYYAO010000000
VERSION     DAYYAO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.191"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            494..715
                     /locus_tag="ACHARI_002896"
     CDS             494..715
                     /locus_tag="ACHARI_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443840.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            716..973
                     /locus_tag="ACHARI_002897"
     CDS             716..973
                     /locus_tag="ACHARI_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443841.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="ACHARI_002898"
     CDS             complement(1108..1710)
                     /locus_tag="ACHARI_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8443842.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="ACHARI_002899"
     CDS             complement(1777..1932)
                     /locus_tag="ACHARI_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFM8443843.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          677 a          463 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc ccctcctccg cccaattcat aaacaccaaa
      121 accaaaaaac aaacctcagg gctccgcccc gaaccccgac ctccttctac cgaaatatat
      181 gcaggcgcca cgcttactat ttcgttcagc tatcggcttt tgcaatctaa cacgaccgtt
      241 tcgctaccag tcgccttaca ggctccattc tcgcttctct tacgtgtttt aacgattgcg
      301 ccgataacta aacgaaatag acgctaaatc gcttaaaacg caaaatagac cctttttaga
      361 gcattgattt tttctactag tcatttcgat aatataaaat tgaccacaac acagaaaact
      421 tatattataa gcttatgtcg taagtccgac gtactcacgt aatatagacg gacgttaaat
      481 ggaaggagca taaatgagag tgataaaatc agaaaaattc actttggccg aaattatgac
      541 gaccagcgaa cttgcacaag aacttgatta caaccaagct tacgttttgc gcttagcgaa
      601 agaacatctg actgaaggaa aagaatatcg ctctgccggt agaagaaact atcttttcag
      661 cccagaagcc cttaaaaaac tagaaaaagt cataaccacc aaagaaggtg agtaaatgcc
      721 taaagaaaca actatcaacg ttgaacaact agaaaaattg ctagaatcat attccaatga
      781 ttttatcgaa ttgaccggcg gaaaagtcga agaaaccacc gacctctttg acctacttct
      841 cacaaatatt ctggaattaa actctgaaga acaaaaagag cataagggaa aactgatagc
      901 accttcctac aggaaggtgg ctaagtcggc tgtgccaact ggttttctta caaaacaacc
      961 ttatattttt tgatatcaca aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAYYAO010000184         2033 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000184 DAYYAO010000000
VERSION     DAYYAO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.192"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1484
                     /locus_tag="ACHARI_002900"
     CDS             <1..1484
                     /locus_tag="ACHARI_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8443844.1"
                     /translation="DILRGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTK
                     RLTVKDLSTNEEIKTDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFL
                     EEKNPQHVVILGAGVMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLK
                     EGLAVHLETRVTEIRDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTG
                     QIIVDDQLQTNLPDIYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMR
                     YRGYIGAGVAKIFDHTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLK
                     LIFDADSGRILGGQAFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDP
                     LNIAGYVAINQMTNIVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNE
                     LRDRINEIPKDKKVYITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          644 a          362 c          401 g          626 t
ORIGIN      
        1 atgatatttt acgaggaggt gtgatcactg accgtgactc gctgatcgaa cgtacaccag
       61 aaatattgaa aacaaaaaac aacatcgatg tctttactaa acacgaagta acagcaatcg
      121 atccttctac taagcgatta acagttaaag acctatccac aaatgaagaa ataaaaacag
      181 attacgataa gttgatcatc tcttctggtg ctagaccaga ttatccggat attcccggag
      241 tttttgaagc agaaaacggc tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat
      301 cgttccttga agaaaaaaat ccacaacatg tcgtcattct tggtgcaggt gttatgggtc
      361 tggaattagc tgagaatttc aagcatcgcg gcttagacgt gactttaatc gatcaattgc
      421 cacaagtcgc tttcccttat gatccagaaa ttgctaattt agtttatgac aaattgctga
      481 aagaaggatt agccgttcat ttagaaacaa gagttactga gatccgtgat aaaggtcgag
      541 aaatagtatt atcagatggc tccgtccttt ctgctgatat gctaattttt gctgttggtg
      601 tttctccgaa gaatgaagtg gtgaaagcaa ccggcataca attatcagat acaggtcaga
      661 tcattgttga tgaccagtta caaaccaatc tgccggacat ctatgcgatt ggcgatatta
      721 tcgaaacgac tagtgtagtg actggtcagc cgatccaaag tatgctttcc agtgcggcca
      781 atcgtcaagg acatatgctg gcagatattt taaatggtac gcctatgcgc tatcgcggat
      841 atattggtgc aggtgtcgca aaaatctttg atcatacagc aagttatgcg ggaatgacag
      901 aacatgaatt aaaagcatca ggcataacaa attataaaac tgtttttatc actccctttg
      961 accatgccta tttctatcca gaagctacaa gattaaatct aaagctgatt tttgatgcag
     1021 atagcggtcg tattttaggt ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg
     1081 gagaactttc tgtagcaatc accggaaact tgacagtctt tgatttgcct gatttggagt
     1141 tgccttactc tccaccgtat tctactaccc gtgacccgtt gaatatagct ggttatgtcg
     1201 caatcaatca aatgacgaat atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg
     1261 atttgaaaga agcgttcttt ttagatatac gtgaacctaa taaagcacct tctggcagta
     1321 tctcggcaac aaaaaatatt ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa
     1381 aagataaaaa agtttacatt actttcagaa gaggattgaa tacttatact tctgcccgaa
     1441 tcttggcagg tttgggtatc aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg
     1501 ggtagccgcg gtctaggagt tatttttact tgaggagtaa aaatgaaaaa gtatataggg
     1561 ttgttggttg gtatgtatat agaatagaag ataaatgtga agaaattgtg cattatttct
     1621 tcacatttat taggttatag tttaagaaag tttaaacatt gcgcaattta attattttag
     1681 ctgtttcttt tcataaaaaa acatgttgtg actctgagac aaatcatagt ccatagtgat
     1741 gtctctagag taactacgcc taatattacg aggcatcata ttaatctatc agcactagta
     1801 cctaaactag catccctttc gaatattttt tcttatataa taaagtttgc tttcactcct
     1861 ctaatctcac caaatcgact ggcttgcgtt gtgtgatact aggaactgat tggtttagaa
     1921 cgacttttct attgtaatca tatttttcgc catcctcgtt gtcttctaat ccttcaatga
     1981 atgatcccta atgtgtgtat taaataattt aatttttttg ttcctattta ttt
//
LOCUS       DAYYAO010000185         2004 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000185 DAYYAO010000000
VERSION     DAYYAO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.193"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHARI_002901"
     CDS             <1..378
                     /locus_tag="ACHARI_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8443845.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHARI_002902"
     CDS             426..1826
                     /locus_tag="ACHARI_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8443846.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DAYYAO010000186         1858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000186 DAYYAO010000000
VERSION     DAYYAO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.194"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            327..1778
                     /gene="cls"
                     /locus_tag="ACHARI_002903"
     CDS             327..1778
                     /gene="cls"
                     /locus_tag="ACHARI_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8443847.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT          593 a          361 c          331 g          573 t
ORIGIN      
        1 tttttgatct tggccctagt cctgtctgaa aaaaaagaga cctaagacaa atacgcattt
       61 gtcttaggtc tcaccatttc atacaatacc agaaaaccat ttggttaact tactgttgat
      121 attatcgcaa caaacgttgc cggttactcc cctaaggaga tattcaactg tactaatatc
      181 ctaccttatt aacctacaaa aagcaactac tttagcccga tttacctaca ttttcatgaa
      241 aggagtgaaa agtatattca ccttggtagg cttctttctt ctatcatgct atactaatgt
      301 tattgtatgt aaggaggaac atttttgtgg tatctagtat tataaccgcc ctttatctat
      361 taaatgcact tattgctttg gtggctattt tgatcaaacc ccgagatgta gcagccattt
      421 gggcatggct cttagtattg tttgcccttc ctggggtggg ctttgtatta tatttatttt
      481 tcggacgtgg attaacggat aagaaaaaat tctatctccg acaaagtgac ttgaaagaat
      541 tagaaaactt tcagtctttt agaggagata ccattgaaca ttacgatcct gacatgggcg
      601 ataaagacaa acagcaattt gttgacttct tctcttcatt aaatcgtatg ccgctgacaa
      661 gaatgaattc tgtcactctt ctcacagacg gacaagagaa attggattca ctgcttcaag
      721 atctaaaaaa agccaaacat tcgatccata tcgaatatta cgcatttgtg acagataata
      781 tcggccagca agtcttacat gttttagaag aaaaagccgc agaaggcgtg gaagttcgaa
      841 tattatatga tgcatttggc tctcatggca caaaagcaaa agatttcaat cgtctaatca
      901 aaaatggtgg acatgtccat acatttgtta cctcacaaag ggcattactt cgtttccgat
      961 tgaattacca tgatcaccga aaaatcgttg tgatcgatgg aaagattagt tataccggtg
     1021 gtttcaatat tgccaatcaa tatgtaaata caacaaaaaa attcggctat tggcgcgata
     1081 cgcatatacg gattttcggt gccgcttctt ctttgctcca gcttcgcttc ttaacagact
     1141 ggaacgtctc ggtacctgaa gaaaaaaagg tcggctatca tttgaattat ttctttaaaa
     1201 aagcagatcg agatgaatct aagcttgctg atacatccat ccagcttgtt tcaagcggac
     1261 cgaataacga aagggaacaa atcaagcttt catttatcaa attgattact tctgctaaaa
     1321 aacgtgtttg gatacagaca ccttaccttg ttcctgatga aagtgtcatt gctgctttaa
     1381 aaatcgcaac tgcctctggt gtagatgtga aaatcatgat tcccaacaaa ccggatcatc
     1441 cttttattta tcgagcaaca caatattatg ctcggcagct gatcaaggaa aatgtacaaa
     1501 tccttgtcta tgagaacggc ttcctccatg caaaaacatt gataatggat gatgaaatct
     1561 gcatggtagg ttcagcaaat caagatattc gtagctaccg attgaatttt gaaacaagtg
     1621 ctgtcattta cgatcctgag tttttagaag aacttgctac tcagttcaaa gaagatgaga
     1681 cacattgttc atccatgaca actgaaacag tcaaggaaat gtctaactgg ctattattca
     1741 agcaacaaat ttctcgatta ttttctccaa tcctataatt tttttattca agtagagaaa
     1801 aatatactaa gattaaaggg actgtgacaa aaatcttgtc acagtccctt cttccgaa
//
LOCUS       DAYYAO010000187         1824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000187 DAYYAO010000000
VERSION     DAYYAO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.195"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            656..988
                     /gene="traE"
                     /locus_tag="ACHARI_002904"
     CDS             656..988
                     /gene="traE"
                     /locus_tag="ACHARI_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8443848.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1607..>1824
                     /locus_tag="ACHARI_002905"
     CDS             1607..>1824
                     /locus_tag="ACHARI_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8443849.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
BASE COUNT          627 a          261 c          375 g          561 t
ORIGIN      
        1 caacacaaaa aagagaacaa acgtttgcat gttgctgttt tttagttata ataatacgcg
       61 aagagaggta atttcatttt ttaaaaaagc taaaaacctt gatattactt cttttcaggc
      121 aatacgaata ttattcgctt acattttttt gtataaattt agttgtttta aatttaattg
      181 tctgctacgc ttgtgatgaa ttaattttcg ttctgcactt ttggacgata aatttggaca
      241 attgaaaata caaaaaaaag aaaggccctg tgagactcgg aaggctggta accttcgtcg
      301 acgtccacag gtggcattaa gacaaccttt aatcccttaa tgcgtttacc tttcagtgtt
      361 acggtattac ccgcaacgga tttagtataa cagatattca aaagtgatac cacttttttt
      421 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
      481 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
      541 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
      601 aaaataacaa gaaaaactta ttaaaaatat ttaaagtaag tgggtggatt attttatgtc
      661 atatacagga attttgagtc tcgaagatat ttgtcactac ggtaaacggt gtaccgctac
      721 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
      781 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacgctg gtaaacaaaa
      841 gcaaataatt ttaaaagatc ctatcccttt aaaagaacta tatccaacaa tcaaacatgt
      901 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
      961 aaaagggatg gggcgtttaa tcagctgaat tgatcattct cataacttgt attttatcgt
     1021 ttttcgcttg cttgaaaata ttgtcgagac ttctaaaaga acttagaatt actagaaaaa
     1081 taaggagtag acttgatcta tcaacaattc ctttcggggg gtgacggggt aggtttttac
     1141 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgccc ttgtttggat agtggtgaaa
     1201 tgaatttgtg taaataatat gcacagagta aacaagaggg aatttagaag ttagacaagt
     1261 tagaatagcg cctagattaa tcatgagcag ctactaaggg atctaggctt tgacgcaaac
     1321 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     1381 tgctgcagta catgtagata cttgcgagga cgttggtcat tggatcaatc gagcatttac
     1441 cgatttctac tagggtttct cgtttcggac gccataaacg agtcgatatt ttttatgtat
     1501 ttttaagtaa acgaaaaacg ataaaatatg aacgagtcct gaataaagtg aaaaaattga
     1561 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     1621 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     1681 aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga
     1741 cgaggatgat ttatactatc aaaaattgac tgataatcca attggattcg gcttgctacc
     1801 tgaagatgat gaaggtaatg aatg
//
LOCUS       DAYYAO010000188         1820 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000188 DAYYAO010000000
VERSION     DAYYAO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.196"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1490
                     /locus_tag="ACHARI_002906"
     CDS             join(328..604,604..1490)
                     /locus_tag="ACHARI_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8443850.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTTNVLDRTFTQEQPLEAIV
                     TDLTYVRVRKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDSQTIHEILNGFGITRSLSRKGCPYDNAVAESTHKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLCLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            1596..1811
                     /locus_tag="ACHARI_002907"
                     /pseudo
     CDS             1596..1811
                     /locus_tag="ACHARI_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          628 a          338 c          356 g          498 t
ORIGIN      
        1 attcctcctt tattttttac acaatagcat tttatagcta ctttcaataa cattctacac
       61 accttttatg aaacgttgat gaaatataat tttttatttt tccaaactat ggtataacaa
      121 tataagtatt atgcttaaag ttgttcttac acgattagta tatgatcgat taatatatga
      181 gcaagcacag taagtgtacg gacacttacg aaaaaattga tgaagcagcc ctttgggatc
      241 tgcttcatca atttgaatcg gctacactag actagacaga aaaaataagg tgtgtagaat
      301 aataaaaaac acactggagg atttttcatg tcaagacgtc aacgaagaac ctattcaaaa
      361 gaattcaaac aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt
      421 cgagagtatg agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg
      481 ggctcattca aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga
      541 aagaaaaata agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc
      601 ggacgaaaag acaagtaatt gatgccaaca agcataaata ttccatatca gcgatgtgca
      661 aaattctaaa tatttctcgt cagacctact attatcaagc gaaaccgatc gaaaatgagt
      721 ccgacttaga agagatcgtt caggaagagt ttattcgaaa ccgaaaggct tacggtaccc
      781 gaaaattgaa gaagtgttta gcaaagcgtg ggcttcaact cagtcggcgc cgaatcggtc
      841 gaatcatgaa acgccgcgga ttgacatcta cctatacgat cgctcatttt aaagggcaac
      901 gaacagcttg taatgaagcg aaaacaacga atgtattaga tcggaccttt acacaagaac
      961 agccattgga agccatcgtt acggatctta cttatgttcg cgtgaggaaa aagtggcatt
     1021 atatctgctt aatacttgat ttgtttaatc gagaaattat tggttattcc tgtggtgaga
     1081 agaaagatgc ctcattggta aaagaagcct ttggacggat accgtattct ttaacagacg
     1141 tcaagctttt tcatacagac cgtggaaagg aatttgatag ccaaaccatt catgagattc
     1201 tgaatggttt tggaattact cgttcattga gtaggaaggg ttgtccgtat gataatgccg
     1261 ttgcggaatc aacccataaa tctgtcaaag tagaattcgt gcatcaatac caatttgaga
     1321 cactggcaca gctacgtcta gaattgtttg attatgtgca ttggtggaac tatctatgct
     1381 tacatggcac gttggcgtat gaaacaccga tccaaattcg acaacagaga ttggcgaagc
     1441 gaatccttga taatgagcgc ggatctgata cctctggaga ggcagcgtaa ttgaatgatt
     1501 gtgcttctgc cggagaaaat cagatccgag gatgctcatt gtcaagggca atcggagcca
     1561 taacaccgca gcattcacaa caccttataa tttttgtcaa aaaaagtgtt gccattccaa
     1621 ccaatctaat cgagtctttc aataagcaaa ttaaaagata cagccgtaga aaagagcagt
     1681 ttcaaaatga agaatcacta gaacgctttc tagtcagcat ttttgataca tacaatcaaa
     1741 aatttctaaa cagaagccat aaaggttttc aacaggtaac cgatacatta gtttcaatgt
     1801 ttactgagta actaattatt
//
LOCUS       DAYYAO010000189         1808 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000189 DAYYAO010000000
VERSION     DAYYAO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.197"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACHARI_002908"
     CDS             275..1753
                     /locus_tag="ACHARI_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443851.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          654 a          252 c          322 g          580 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gttttttt
//
LOCUS       DAYYAO010000190         1750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000190 DAYYAO010000000
VERSION     DAYYAO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.198"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1292
                     /locus_tag="ACHARI_002909"
     CDS             123..1292
                     /locus_tag="ACHARI_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8443852.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1634..>1750)
                     /locus_tag="ACHARI_002910"
     CDS             complement(1634..>1750)
                     /locus_tag="ACHARI_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8443853.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          609 a          289 c          290 g          562 t
ORIGIN      
        1 ttttacactc tctattatga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1381 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1441 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1501 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1561 ccatggataa taataagggt tctgttgcaa agttttaaat aaagaataaa atcccttata
     1621 cggtatctat gatttaagct gggattccca ataatacctt gatttcagta cagaccgaaa
     1681 acccgaagag agtgccttct tttcgggttt tcttatataa tcctcggatg gcttccatgc
     1741 ctttaatcgt
//
LOCUS       DAYYAO010000191         1718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000191 DAYYAO010000000
VERSION     DAYYAO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.199"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..500
                     /locus_tag="ACHARI_002911"
     CDS             <1..500
                     /locus_tag="ACHARI_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443854.1"
                     /translation="SIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGK
                     KETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFE
                     KTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLV
                     EYGII"
     gene            487..>1718
                     /locus_tag="ACHARI_002912"
     CDS             487..>1718
                     /locus_tag="ACHARI_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8443855.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKIT"
BASE COUNT          554 a          224 c          273 g          667 t
ORIGIN      
        1 aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac
       61 ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta
      121 tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt
      181 atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg
      241 acttctcttc aacacttttg attagtccta tatctacaat ttctttttca ccatctatga
      301 tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag
      361 cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata
      421 accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg
      481 tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg
      541 tatactatag ggaatttttt tattaaggga atttcattca taactattcc tatttttgta
      601 aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt
      661 ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat
      721 aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt
      781 gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac
      841 caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc
      901 cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct
      961 acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa
     1021 tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat
     1081 ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta
     1141 aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat
     1201 agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt
     1261 aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg
     1321 ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat
     1381 ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa
     1441 tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc
     1501 tatttctttc agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca
     1561 tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt
     1621 ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac gtatttttta
     1681 ctttttattt ttcattataa tatcgctaaa aaaattac
//
LOCUS       DAYYAO010000192         1646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000192 DAYYAO010000000
VERSION     DAYYAO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.200"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..412
                     /locus_tag="ACHARI_002913"
     CDS             <1..412
                     /locus_tag="ACHARI_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443856.1"
                     /translation="FILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELTPLDR
                     NGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKE
                     KRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            422..625
                     /locus_tag="ACHARI_002914"
     CDS             422..625
                     /locus_tag="ACHARI_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443857.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            836..1420
                     /locus_tag="ACHARI_002915"
     CDS             836..1420
                     /locus_tag="ACHARI_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8443858.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          577 a          238 c          301 g          530 t
ORIGIN      
        1 gtttatttta tctttaggta ttttaactat tggaattagt gtaactggaa ctacaattcc
       61 tacatttgct gatagtacaa atgaaccaac tactgtagta gataataacc aagaattaac
      121 tcctttagat aggaacggaa tagccattgg taacccaact aacgacttaa ctgttactga
      181 aaattattta atagtttctc cattacttgc tcgagcagct tctaaaacga tacgagtgac
      241 aggaacgatg atttttgata aagttggttg gccttctaga cctcctcaat cacggaattg
      301 gaaagaaaaa agaaatggtt attggtacta tggaaaattg tatttacaaa gttataaatc
      361 agctggaaac aattggattg cgacttattc aggaacctta actagaaagt agggattttt
      421 tatgccagaa tttgtgaaca caccaaattt ttatcaagat agcgatattc taagtaaaat
      481 taatttaccg aaagaagaat cagctttaca agttcagcga actttacagg agacacaaaa
      541 aattcaaaaa tcaatttcta atctagaaga ttctgaagaa aaaagaaaaa aaagagacca
      601 aaaaacatat caaaaagaaa aataatgatt ttaaaaaaag agtgtctagt cgacattctt
      661 tttttctaat tgaaagtgtt cataaaatca cccatttctg gacactttct ttttttgctt
      721 tatttttcaa aaaaacaatt catttcatgt tcataaaccc ataacaatat ctatgttcag
      781 ttaaactata tggataaagt tatgttacaa taaaattaat gaaggagtga tttttttgaa
      841 aataggatac gcccgggtat ctactggttt acaaaatttg gatttacaag aagacagcct
      901 aaataaattt ggttgtgaaa aaatttttac tgatcatatg agtggggcaa aaagtaatcg
      961 accaggatta gaaatggcca ttgattttgt tcgctctggc gatacacttg tcgtttggcg
     1021 cttagatcgt ttaggtagaa atatggaaga tttgatttca atcgtcaatc gactgaatga
     1081 gcgtggtgtg agttttcata gtattcaaga aaatattacg atggacaaat caagttctac
     1141 aggacagctc atgtttcatt tgtttgcggc atttgcagaa tttgagcgta atttgatcct
     1201 ggaacgatcc gcagcaggac gagcagctgc tcgtgcaaga ggacggtttg gtggtcgtcc
     1261 tgaaaaattg tctgaacaag atttggaatt actgaagact ttagtggata gcggtacacc
     1321 tattaagact attgcggaac ggtggaacgt ctcaagaacc accatttatc gttatttggc
     1381 aaaaatggag gagaaaaatg gaaagacaaa ttctcattag tttttgtcat ttaatgctga
     1441 aaatttttgc catttatcta gaaaagtgac aatagtatga gaattttttg taagtgtcta
     1501 ctgataactt attctcaaat gttgtaagga ttttattcat ttgttaatat taaagaacgg
     1561 aactccaaaa aagtccgtac tctaatttgc agtctctcac tcttttagag tgagagtttt
     1621 tattagattt tcaaattaat tagtaa
//
LOCUS       DAYYAO010000193         1623 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000193 DAYYAO010000000
VERSION     DAYYAO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.201"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..406
                     /locus_tag="ACHARI_002916"
     CDS             89..406
                     /locus_tag="ACHARI_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8443859.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="ACHARI_002917"
     CDS             complement(497..862)
                     /locus_tag="ACHARI_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443860.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="ACHARI_002918"
     CDS             1070..1552
                     /locus_tag="ACHARI_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443861.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          543 a          219 c          279 g          582 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 att
//
LOCUS       DAYYAO010000194         1512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000194 DAYYAO010000000
VERSION     DAYYAO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.202"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1463
                     /locus_tag="ACHARI_002919"
     CDS             <1..1463
                     /locus_tag="ACHARI_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8443862.1"
                     /translation="KQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVA
                     NGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLA
                     LPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALL
                     APLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKG
                     FIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAE
                     EKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIF
                     IFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWL
                     LGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTP
                     GTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          413 a          289 c          318 g          492 t
ORIGIN      
        1 aaaaacagca agacaagggc ttcttcgggc agccgaaggg attgtcaact ttgttcttca
       61 ctgagatgtg ggaacgattt agttattacg gtatgcgtgc attacttatc tattatatgt
      121 acgatacagt tgccaacggg ggacttggat taccacgagc aacagcatta gctatcatgt
      181 cgatctacgg atctttgatc tacatgtcta gtatcgtcgg cggatgggta tctgaccgag
      241 tgttaggagc aaaacgcact gtattcttcg gtggagtttt cattatgctt ggccacatcg
      301 tcttggcttt accatttggt gtaagtacat tgtttgtttc gatggcattg attattatcg
      361 gaaccgcctt tttgaaacca aatgtttctg gcatggtcgg acatctttat tcaaagactg
      421 acttgcgaag agatgcagga ttttcgattt tttacatggg tgtcaactta ggggcattat
      481 tagcaccact aatcgttggt acaatcggtc aagaatataa ctatcattta gggttctctt
      541 tagctgctat tgggatgttc tttggattat tacaatacat catccaagga cgcaagacat
      601 tagacggagt gggtacaaca ccgccgaatc ctttgacaac tgaagaacgt aaaggattca
      661 tccgtaactt gatcatcgca cttgttgtta tcgctgcagt attcggcggg gcacaactaa
      721 ctggtcatct gacaattgac ttttttgtca atacaatcag tgtattgggg attatcttac
      781 cgttgtatta ctttatcaaa atgctgactt caaaagatgt aactgctgaa gaaaaaccaa
      841 gagtatgggc ttatatccca ttattccttg cagcaatcat tttctggtca ttacaagaac
      901 aaggttcatc tatcttagct ttatttgcta gtgaacgtac acaatctacg ctatttggtg
      961 taacgatcca agcaagctgg tatcaatcat tgaatccaat tttcatcttt attctgacac
     1021 cagtattcgt aacgatgtgg acgaaacttg gtgaacgtca accatctaca gtagtgaaat
     1081 tctctcttgg acttttattt gcaggattat cgtacttgct attgatgttg ccgggtatgc
     1141 tttatggtac aagtggaaaa gtaagtccat tgtggttgtt gggaagtttc ttcatcgtgg
     1201 ttattgctga attgtgtctt tcaccagttg gtttgtctgt gacaacaaaa ttagcaccac
     1261 gagcatttga atcacaaaca atcgctattt ggttcttagc tgatgcttct tcacaagcaa
     1321 tcaatgcaca aattgcacgc ttctacacac cgggaacaga atctgcttac ttcggtatta
     1381 tcggtgcagt agcaatcgtc ggtggtatct tgctatttac tgtgaaagaa ccaatcaaaa
     1441 aactaatggg ctctattcat taatcaatga acataaaaac aggaattgct tatttgcagc
     1501 agttcctgtt tt
//
LOCUS       DAYYAO010000195         1493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000195 DAYYAO010000000
VERSION     DAYYAO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.203"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(606..962)
                     /locus_tag="ACHARI_002920"
     CDS             complement(606..962)
                     /locus_tag="ACHARI_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8443863.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="ACHARI_002921"
     CDS             complement(976..1386)
                     /locus_tag="ACHARI_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8443864.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          459 a          274 c          212 g          548 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta aatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acg
//
LOCUS       DAYYAO010000196         1482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000196 DAYYAO010000000
VERSION     DAYYAO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.204"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..1007)
                     /gene="thiD"
                     /locus_tag="ACHARI_002922"
     CDS             complement(192..1007)
                     /gene="thiD"
                     /locus_tag="ACHARI_002922"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8443865.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1301..>1482
                     /locus_tag="ACHARI_002923"
     CDS             1301..>1482
                     /locus_tag="ACHARI_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443866.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFM"
BASE COUNT          434 a          303 c          238 g          507 t
ORIGIN      
        1 agaagctgtt ttttgaactt gttccttgtg actcggagct ggacggcagg ctcacgacct
       61 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa
      121 cataagaagc tgtttttact tggctgttct tattacttga tgcagggcaa ctttttcaca
      181 acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc atatttgtgc
      241 cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa tcggatcgct
      301 tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag cgaatcgcca
      361 gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga ttttcctgat
      421 tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg atgtcctttg
      481 atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac aaaatcttct
      541 tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg tgtcagtact
      601 tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc tagtaaaaga
      661 tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc atatttttgg
      721 taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt tttagctgca
      781 cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc tgttggcaat
      841 gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat gacagaaaca
      901 ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc gcttccatcg
      961 ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa atcgattcct
     1021 ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat gtttttcaaa
     1081 gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt tgaaaaaaca
     1141 tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta ataataaata
     1201 accactattt gatttatata tatttcagta tccttcttgt tttcatataa ccaaaggcgc
     1261 tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg aggataaaaa
     1321 agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata cgatctgttc
     1381 tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag gaataatcta
     1441 tttcctacat gaagaaggtt acgataccca aacctttatg tt
//
LOCUS       DAYYAO010000197         1466 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000197 DAYYAO010000000
VERSION     DAYYAO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.205"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACHARI_002924"
     CDS             <1..277
                     /locus_tag="ACHARI_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8443867.1"
                     /translation="EVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQ
                     CGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            282..1229
                     /locus_tag="ACHARI_002925"
     CDS             282..1229
                     /locus_tag="ACHARI_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8443868.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          487 a          205 c          302 g          472 t
ORIGIN      
        1 tgaagttact atagttaata aatcaagata cacatctagg caacagacac ctgaagtttt
       61 tgaaatgaat gcctcaatgt attcttttaa acctgaattt ctaaaaaaaa gtaattatat
      121 ttttgatggg caatgtggga taataaaaat gagagattat ctaattttag atattgatag
      181 tgaagaagat tttaaatggt tagaattttt gtttcctaaa tttttagaag attcttcgga
      241 gcttcaagat atctataatt atttatatcg gaggtaaaag gatgagctgt tatcttatca
      301 ccggtggggc tggcttcatt ggttctacct tagccaatca tttaggagaa gaacatgtgg
      361 taatcgtcgt cgatgactta tcaatgggaa aagtagaaaa tcttgatatg acgaaaaata
      421 tcacttttat tgagggagat gtcgctgaga aatccttgat ggaagagatc atgaaagcta
      481 ataaatttga ttatatcttt catttagcag cagttgctag tgtggcagat tcagttgccc
      541 gaccagtaga aacacatcgt gtgaattttg aaagtgtatt aatgatttta gaattgatcc
      601 gcaagtatca acctgattta aaacggatcg tgtttagttc ttcagcggct gtttatgggg
      661 atgaaccgac tttaccaaaa aaagaagagt ccgtgattcg cccattgacg ccttatgcgg
      721 ttgataaatt cgctgcagaa caatatgtgc tagattattg tcatttatat gatgtacccg
      781 gtagcgcagt tcgttttttc aatgtctatg gtccaaatca aaatccgaat tctccataca
      841 gtggtgtgat ttctattctc gtagatcgct ataagaaaca aatcgtagga gaagcaactt
      901 catttacatt atttggtgat ggaagccagt ctagagactt tgtttatata gatgatgtaa
      961 tccaagcatt acttttagtt gcgaatgagg aagctgcact ggggaaacaa ttcaatgttg
     1021 gaacgggtaa agcaaccaca cttttggaat tgattcatgc aatcaatgaa attctcggtg
     1081 tagaattgac acttgaatac aaagaagagc gttcgggtga tatccatgac tctctagcag
     1141 atattactaa gatccagtca attggctatc aaccgaaata tggtatttta agtggaatga
     1201 ctaattattt gaaaactgaa atcaattaaa acatactaag ataagattag agggtaaatt
     1261 caggctcttt gtcaataagg actgatgagt tttgtaaaat caaatctggg atagaagggc
     1321 tctttgtcaa atgatgttgg taaagagaaa tcttagaata atttggacag aacagaaaga
     1381 agaaaacctc tttctgttct gtttatttgg ttaaaaatgt tttgccatga cgaatcataa
     1441 ttcgtagttt gaaattgtaa aagttg
//
LOCUS       DAYYAO010000198         1413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000198 DAYYAO010000000
VERSION     DAYYAO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.206"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..398)
                     /locus_tag="ACHARI_002926"
     CDS             complement(108..398)
                     /locus_tag="ACHARI_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443869.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(516..776)
                     /locus_tag="ACHARI_002927"
     CDS             complement(516..776)
                     /locus_tag="ACHARI_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443870.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(913..1326)
                     /locus_tag="ACHARI_002928"
     CDS             complement(913..1326)
                     /locus_tag="ACHARI_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8443871.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          517 a          235 c          209 g          452 t
ORIGIN      
        1 taaagtggat tgatttgaga tctttttata cttcaaaaaa gataacaccc aactttcaaa
       61 aatatgtttt ggaagttggg acaaacgagt taatacattt taatacatta ttttttattc
      121 aaaaaaaata gcacaatggc aaagatcgta agagagatca taagccccaa tgaatgacta
      181 aaaagaaaag cagaatcaca tgccatatca tcccatattt cttcaagaaa ataaataagc
      241 gggaaaaagg gaaggaatag tagaacatcg atccagcacc atttttttct tcttttgaaa
      301 taacataaaa caataaacat gaacgtgcag ggaagtaaaa acaaagcttt actcataact
      361 aacaaaatac cagctccttt tatttttttc tcccccacat tcaaagagta acaatggtat
      421 gtacttaata atagaaaaat ggatcaactt gtatgaataa cagctgaaaa gatctgcacc
      481 aaataaaaaa cttccagcca aaactgactg gaagtttatt tttttctgaa atattctaac
      541 acaaatatga aaaagatggc cgaagctaat aagccgattt cgtagcaaaa caatgagata
      601 ccagagtcgt aataataaaa tcctctaatc ttatggataa aatagatcat cggacaagca
      661 gataagagta agtatttgtt aggccttttg aataagtaaa agaaagcact ggtcattatg
      721 ataaatggta tccaaaacaa aagtttactt aagacaatca aatgatcaac tcccatattt
      781 tttaccaaga gagatcataa catctttatc gtaaaaacaa aatatttttt tgctcatttt
      841 gaaaatttaa acatactcgt tcttatccaa atatataaaa atcgcccctc attcatcgag
      901 aggcgataag ttctaattta actcaatttt ttttgtttct tttggctgat aagctgtttt
      961 aggtaatacc aatttcagca caccgttttt aaaggttcca gtgatttttt cttcatcaac
     1021 attaggaaga tagaatgaac gtttgtaaga gaccgaatgt ctttctttcc gtacatagtt
     1081 gccatcttct ttatcttctg tatgggactc atgattggcg gagatcgtca aagtattatc
     1141 tgaatattca actgtcgtgt cttctttgtc acagccaggc atatcagcag ttagtttgta
     1201 ttctttttca ttttcgacta aatctacttt tggataactt gaaacaccta aaaaatcatt
     1261 gaatgctgga ctaaacacgt cattgaaatc agggaacatg tcgcgaatgc taggtacatt
     1321 tgccattttt gatcatcctt tcaataagta ttttttattt atccctattt acgtaataac
     1381 tctattttga aaaaaatgca agtataatgg tta
//
LOCUS       DAYYAO010000199         1363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000199 DAYYAO010000000
VERSION     DAYYAO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.207"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1016
                     /gene="scrK"
                     /locus_tag="ACHARI_002929"
     CDS             144..1016
                     /gene="scrK"
                     /locus_tag="ACHARI_002929"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8443872.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          431 a          235 c          312 g          385 t
ORIGIN      
        1 tagaatttta aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat
       61 gttactaaat aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt
      121 ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat
      181 ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa
      241 cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt
      301 tagcagggat tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt
      361 atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc atcggtacca
      421 tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg gctgcctatg
      481 gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat acgatcggta
      541 cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt agtcatccag
      601 aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc agctgtccat
      661 ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaac cgcttaggga
      721 aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc tcctatattg
      781 ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt tttggcggtg
      841 gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca aaattggtca
      901 atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca ttagaagaca
      961 acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat tcataaacga
     1021 aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa tttagtcata
     1081 accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa gccttgaatg
     1141 agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac ttgtattgtg
     1201 caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt ttaacaaata
     1261 ggttctgttg caaagtttta aatctactat cagataaggt agaataatag aaaaagatag
     1321 caggaggaat gacgatgagt cattttaaag gaaagcaatt tca
//
LOCUS       DAYYAO010000200         1362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000200 DAYYAO010000000
VERSION     DAYYAO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.208"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACHARI_002930"
     CDS             82..>1362
                     /locus_tag="ACHARI_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8443873.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAYYAO010000201         1337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000201 DAYYAO010000000
VERSION     DAYYAO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.209"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1337
                     /locus_tag="ACHARI_002931"
     rRNA            <1..1337
                     /locus_tag="ACHARI_002931"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAYYAO010000202         1332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000202 DAYYAO010000000
VERSION     DAYYAO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.210"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(317..1207)
                     /locus_tag="ACHARI_002932"
     CDS             complement(317..1207)
                     /locus_tag="ACHARI_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8443874.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          337 a          287 c          241 g          467 t
ORIGIN      
        1 ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc ggcgcacaga acgcgccgca
       61 tatcggtcta agttttattc aaatttgtct tcaccaacac gatttgacga agagccaaaa
      121 aaagttacga cagttctatg tcgtagctct ttttttcgaa taaacggtgt tcaatcagct
      181 gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttc acaattaaag
      241 gaaaaatgta tatgtgggga aacatagttg atttttcctt aatagtaacg actaccgtgg
      301 catgtttatc tgctagttat ttgatatttc cttcgtgatc acgttctact tccatcattg
      361 tgattggtaa atagcccaat tctttgacag gtccttcttg gatttcttct gtcatcatgt
      421 agtctaagaa tttttggact tcaggggaag gttttccgtt cgtgtacata tgttcatatg
      481 accagattcc ccagctgtta tctgctacat tctcttcttt tggttctaca ccgtcgatac
      541 ttagtgcttg tgtggaatca tcgagatatg agaaagctaa atagctgatt gctcctggtg
      601 tttgacttac cagctgccga actgttcctg atgaatcttg ttcttgggac tgcacagggg
      661 tagcaccatc cagtccccat ttttcgaatg tcgcgcgggt accgctgccg tttgcacggt
      721 tcacgacgtt gattttttga tctttcccgc caacttcctt ccagttagtg atttttcctg
      781 taaagatatc aatcaattcc tgtttggtga tatctttgac atctgtatct ttattgacga
      841 ttggtgccat cccgaccact gctactttat gatccactaa ttttgaagca tcaactccgt
      901 ctctttcttc tgcaaataca tcagagtttc cgatagtaac tgctccagct tcgacttggc
      961 tcaaaccagt tccgcttcct cctccttgta cagagatttg ataatttgga ttttcttgta
     1021 caaagctttc ttgtgctgca tcgactaacg gctgtaatgc tgtcgaacct accgcaacga
     1081 tttttacttg ttcatttgag tcacttgaat tattttgtgt ggcagagcca tctgtatttc
     1141 ctgatcctga gccgcatccg gctaaaagaa ttcccatcat acctagagta aacaatactt
     1201 ttttcatttt attgctcctt tcttatttac aactaaagtt tatcagtcga ctgtaaatcc
     1261 ctcgtatggt tgctgtaaaa atatagttaa gttatgtaaa aatcatgtaa acgtttttcg
     1321 cattctttac at
//
LOCUS       DAYYAO010000203         1331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000203 DAYYAO010000000
VERSION     DAYYAO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.211"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..595)
                     /locus_tag="ACHARI_002933"
     CDS             complement(<1..595)
                     /locus_tag="ACHARI_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8443875.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQAL"
     gene            946..1245
                     /locus_tag="ACHARI_002934"
     CDS             946..1245
                     /locus_tag="ACHARI_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8443876.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          480 a          191 c          220 g          440 t
ORIGIN      
        1 caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt acttcaatca
       61 tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa aagctagcat
      121 tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca aaatctgcgc
      181 ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat aaatttacat
      241 agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc aatttagtac
      301 ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc aatatacagc
      361 ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga aaatcattgg
      421 aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag attatttggt
      481 tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg ttttcataga
      541 tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa atcatattta
      601 attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac attagcagaa
      661 aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt gaaacgttct
      721 tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt agaataaaaa
      781 tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata acagtgtatt
      841 gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt atactttgtt
      901 tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc taaaagtgaa
      961 gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga gatcaaagaa
     1021 aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca gtattatcct
     1081 aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg agaattgtct
     1141 aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga aaaagaatta
     1201 ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg agttattcat
     1261 ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat ttatttgaac
     1321 agagtctgta t
//
LOCUS       DAYYAO010000204         1321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000204 DAYYAO010000000
VERSION     DAYYAO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.212"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..355)
                     /locus_tag="ACHARI_002935"
     CDS             complement(2..355)
                     /locus_tag="ACHARI_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443877.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSESN"
     gene            complement(452..>1321)
                     /locus_tag="ACHARI_002936"
     CDS             complement(452..>1321)
                     /locus_tag="ACHARI_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8443878.1"
                     /translation="YNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGN
                     LSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSV
                     SGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRD
                     SGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFK
                     NEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQ
                     YQLGLVDNFNNIS"
BASE COUNT          457 a          227 c          143 g          494 t
ORIGIN      
        1 attaattgct ttcactcaaa tacttataat ctattaactt gtagttgttt aacttttctg
       61 aaaaagttag acccacgtaa taatcttccc agctttctct ttcaattaac tgatccaatt
      121 tttgataatc cataaattca tcatgtatta aaaagaataa ataacttgat attttagcat
      181 ctagcttaat cagattaaat aatgtttgct taaaattgct accattaata tgactgatag
      241 attctctagc ttcttcacat aaacttttta ctttattaat tactactttg tcatattcat
      301 ttaacacact attattacca ttacttagac gctcagattc tacgaaacaa ctcaaatcag
      361 aaatttccca aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa
      421 atgatatagt ctcataataa aataaaacct ctcagctaat gttgttgaaa ttatccacta
      481 aacctaattg atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt
      541 gtgaagaatc tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca
      601 acattatttt agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca
      661 tttcattctt aaagctttct cttaatttag tttcacttac agaaaaataa ttagctattt
      721 cctttacagt aattctttta tagatattta agccgatgaa ttgaacgaca gaagaaatta
      781 agggagaaag atgctctatc ttagtttttc cgatttcctt agtataataa acaatcccag
      841 aatccctcac ttttaagacc tcaggtaacg atacagctaa ttcgttttct tgtatcaaag
      901 catctctact ttgatacgct tctattatat ccattcctaa tgttatagac gtttgtgcta
      961 acttttcaaa aacaatgata gaataatttt tttcagaccg aatcgtatct ccactaacac
     1021 ttggaattat tccattactc agattaaaaa ccatgttttc taaatttcga atatctccat
     1081 ttttaacata ttccagtatt ttattttcat aaagaaacag atatatttct ggatcgtaat
     1141 tttgtgataa cagattatct attttgattt gctcgatagt tttacttaga tttcctgtat
     1201 attcaattaa cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta
     1261 aaatcagaat atctcgaatg tctcctaaag aatacaaagt caaattttgc aatgaattat
     1321 a
//
LOCUS       DAYYAO010000205         1281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000205 DAYYAO010000000
VERSION     DAYYAO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.213"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1256
                     /locus_tag="ACHARI_002937"
     CDS             24..1256
                     /locus_tag="ACHARI_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8443879.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          413 a          246 c          293 g          329 t
ORIGIN      
        1 aatggtgagg aggaattttt attatgttat ctgattttaa tgaacttcta aaaaaatatg
       61 cacgcttaat tgcagaaact ggcgtagcca cagaaaaagg acacacagtt gttttacaga
      121 tcagcgtgga acaggcaccc cttgctcgat tgatcacaca agaagcttat aaactaggag
      181 ccgctgaggt tatcgttcaa tggactgacg accagatcca aagagaattt cttttacatg
      241 ctgcaacgga tcgtattgag aatgtaccac aatctaaaat cgatcaagcg gatgaatggc
      301 ttgaaaaagg agcaagcagg atcagcgttg tttctgccga tcccgatgca tttgctggtg
      361 tagatagcca tcgagtagcg tcttatcaat cagcgaatgg caaagcacta atgaatttac
      421 gcaaggcaac tcaagcaaat aaagtcagtt ggactgtcgt ggctgcggct ggtaaacaat
      481 gggcagcaaa agtcttccca gaactgccag aagaaaaaca agttgaggca ttatgggatc
      541 agattttcaa aacgactcgt gtctatgaag aagaccctgt cttagcatgg aagaagcatg
      601 atgaaaaatt agcaaaaaaa gcagaagaat tgaatcgaga acaattttct gcccttcact
      661 atactgctcc cggtacagat atcattatcg gattgcctaa aaaccatctt tgggaaggcg
      721 ctggaagcta taatgctcgt ggagaaaaat tcatggcaaa tatgccgacg gaagaagtgt
      781 tcacagcacc tgacagtcat cgagtagatg gttacatttc aagtacaaag ccactgagtt
      841 atgctggcac catcatctcg ggtatgaaat ttacttttaa agatggaaaa gttgttgatt
      901 tttctgccga acaaggagaa gatgtcttag caaaactgct tgatacagat gaaggtgcgc
      961 gtcgtttagg agaagtcgca cttgtacctg acccttcacc tatttctcaa tctggcatca
     1021 tctttttcaa tacattattt gatgaaaacg catcgaatca cttagcacta ggttctgctt
     1081 atgccttcag tgtgaaaggc ggtacagaaa tgtccgatga agaattagca gaagctggat
     1141 tgaatagaag tcaaacccac gtagatttca tggtaggatc tgacaagatg gatatcgacg
     1201 gaatccgtga agatggctct acagtaccga ttttccgaaa tggcgattgg gcttgagtat
     1261 actttcgcac acatgcaaaa a
//
LOCUS       DAYYAO010000206         1252 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000206 DAYYAO010000000
VERSION     DAYYAO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.215"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..1111)
                     /locus_tag="ACHARI_002938"
     CDS             complement(407..1111)
                     /locus_tag="ACHARI_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443880.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          368 a          216 c          228 g          440 t
ORIGIN      
        1 gagagactcc tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa
      121 cacaaaatat tatagaaccg cttttttctc tatcagaaaa tatgtttatt attagaattt
      181 tttatagtta aggttaggca tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga
      241 agagaagtcg cctaacattg taggatcata ggtgatttgt tggacaggtc aagctgatat
      301 ttgagcctga tgttagaagg aatttctttt tacttgctgt agaaattatt atatgactaa
      361 aaaggctggg attttaaatc ccagcctttt atgacaaata gaatttttat cgatcagtaa
      421 tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt caatttgact ttgtatttcc
      481 gttcgagtgc tttttcgata acgaactcat tttcagtagt catagaaata gttgctaatt
      541 tacgaggttc atcagcattt ttttcattaa taactacttc atgttcattt tgttctgtgt
      601 aaaatttagg atactcagca acggcttttg aaaaatcagc aaaacttaaa gcggcattcg
      661 tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg
      721 gcacactgac tgtttcttct gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt
      781 ttttcaatcg ttgatcattc cagtaagaca cgatttcaat aagaagattg tcacggaatc
      841 gttcgaaaat cccgttttct cctgaacgaa ttctctttaa tacagtattg aagttgtcat
      901 cgattggcac gttgatccgt gtatcttcat ttgttttccc agctaatccg atcgttaaga
      961 tttgacggtt tttgggatca agcatgactt gtaacggcaa accataacgt cgcaagctct
     1021 cttcttctag agaacggaat aattgtcctc ttacaccatc caatgaagga cggaattgat
     1081 ttaataattt ttgtttctgt tttttcttca tatgttctct ccttcgctca taccctttcg
     1141 attataagaa aaaaacagcc aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca
     1201 aattacaaca gaaaaatttg cgtattttcc cgttttttga aagaaaaata aa
//
LOCUS       DAYYAO010000207         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000207 DAYYAO010000000
VERSION     DAYYAO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.216"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHARI_002939"
     CDS             <1..1076
                     /locus_tag="ACHARI_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8443881.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAYYAO010000208         1187 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000208 DAYYAO010000000
VERSION     DAYYAO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.217"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1102
                     /locus_tag="ACHARI_002940"
                     /note="aph(2'')-Ia"
     CDS             <1..1102
                     /locus_tag="ACHARI_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFM8443882.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSV
                     AYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG
                     YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEE
                     YILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLT
                     GIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEY
                     YPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          517 a           86 c          195 g          389 t
ORIGIN      
        1 tacaagatat attaaattga tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt
       61 tattttagac cctcataaaa ataatccaag agcaataagg gcataccaaa aatctggttt
      121 tagaattatt gaagatttgc cagaacatga attacacgag ggcaaaaaag aagattgtta
      181 tttaatggaa tatagatatg atgataatgc cacaaatgtt aaggcaatga aatatttaat
      241 tgagcattac tttgataatt tcaaagtaga tagtattgaa ataatcggta gtggttatga
      301 tagtgtggca tatttagtta ataatgaata catttttaaa acaaaattta gtactaataa
      361 gaaaaaaggt tatgcaaaag aaaaagcaat atataatttt ttaaatacaa atttagaaac
      421 taatgtaaaa attcctaata ttgaatattc gtatattagt gatgaattat ctatactagg
      481 ttataaagaa attaaaggaa cttttttaac accagaaatt tattctacta tgtcagaaga
      541 agaacaaaat ttgttaaaac gagatattgc cagtttttta agacaaatgc acggtttaga
      601 ttatacagat attagtgaat gtactattga taataaacaa aatgtattag aagagtatat
      661 attgttgcgt gaaactattt ataatgattt aactgatata gaaaaagatt atatagaaag
      721 ttttatggaa agactaaatg caacaacagt ttttgagggt aaaaagtgtt tatgccataa
      781 tgattttagt tgtaatcatc tattgttaga tggcaataat agattaactg gaataattga
      841 ttttggagat tctggaatta tagatgaata ttgtgatttt atatacttac ttgaagatag
      901 tgaagaagaa ataggaacaa attttggaga agatatatta agaatgtatg gaaatataga
      961 tattgagaaa gcaaaagaat atcaagatat agttgaagaa tattatccta ttgaaactat
     1021 tgtttatgga attaaaaata ttaaacagga atttatcgaa aatggtagaa aagaaattta
     1081 taaaaggact tataaagatt gattatataa tatatgaaaa gctattataa aagacattag
     1141 tattaaatag tttaaaaaaa tgaaaaataa taaaggaagt gagtcaa
//
LOCUS       DAYYAO010000209         1137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000209 DAYYAO010000000
VERSION     DAYYAO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.218"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..812
                     /locus_tag="ACHARI_002941"
     CDS             <1..812
                     /locus_tag="ACHARI_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8443883.1"
                     /translation="YFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLT
                     KLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDISMS
                     TFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEK
                     RVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTN
                     NVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            845..>1137
                     /locus_tag="ACHARI_002942"
     CDS             845..>1137
                     /locus_tag="ACHARI_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8443884.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGAD"
BASE COUNT          440 a          123 c          212 g          362 t
ORIGIN      
        1 tttattttgg cgggggaatt aatttatggt tagccaaaaa gaatggaatc agacagagaa
       61 ttgctcatag ccatgcaact gaagatggaa aatctcaaaa tattgtaatg aatgtattgc
      121 gtaaattttt aaccaaattg ttgttacaaa atgcaacaga ctaccttgcc gttagtcaag
      181 aagctggtga aagtctgttt caaaaccacc cctttgagat agttcataat ggtattgatt
      241 tagagcttta tagtaaaaat tcagaagcta aggttaataa gagaaaagaa ttggatatct
      301 caatgagtac ttttgttgtg ggaaacattg gacgattaga gaaacaaaaa aatcaatctt
      361 atttgttaga gattttttta gaaatactaa aaatgaattc tgattcttac ttgattttag
      421 taggtgaagg gtcacttaga aataaactga aagccaaagc agtagaattg gatatagaga
      481 aaagagtaaa atttttagga gaaagaaacg atattcccga gttattatct gtgatggatg
      541 tttttgtaat gccttctctt tatgaaggtc tgccaatggt tggattagaa gcacaagcag
      601 caaaattaaa attagtctta gcagatacta tttccaaaga tacaaagtta acaaataatg
      661 tggattttgt atcattagat gaaacaccac aagaatgggc aaaaaaagta ataaaagagc
      721 cgtatgctaa taaaataaca caagaactaa agagttatga tgtttcgtac actactgaac
      781 aaatggagag tatatatagt agagatcatt aaaaatgttc aagatatttt aggagtgatt
      841 atttttgaaa atatgttatt tgatattggc atacaatgat attggcaact taaagagatt
      901 ggtcaatcga ttgaacgaaa atgcagtttt tattattcat atagataaaa aaactgatat
      961 agaaccattt attgaagcat ttaaatcata tgataatgtt gaatttttat gtaataaaaa
     1021 ccgtgttaaa atttattggg gtggtttttc cattgtacaa gcagaaatca atttagtaaa
     1081 gagagcatta caaaatgaga aatatttaaa atatgtatta ttatctggtg ctgatta
//
LOCUS       DAYYAO010000210         1106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000210 DAYYAO010000000
VERSION     DAYYAO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.219"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHARI_002943"
     CDS             <1..1087
                     /locus_tag="ACHARI_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443885.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DAYYAO010000211         1065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000211 DAYYAO010000000
VERSION     DAYYAO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.220"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1025
                     /locus_tag="ACHARI_002944"
     CDS             <1..1025
                     /locus_tag="ACHARI_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8443886.1"
                     /translation="KKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTPF
                     CFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCLL
                     FDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIVT
                     DMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYRQ
                     LKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDLL
                     TAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLHT
                     GSVAKF"
BASE COUNT          327 a          216 c          202 g          320 t
ORIGIN      
        1 gcaagaagat ctctatgatt ctcctgaatc gtagcggtaa taaacggacc tatcttcggt
       61 tgaagaaaca gcgatacctc tgccgagcat gtaaaaaata ttttaccgct cggacgtatc
      121 tcgttacccc attctgcttt atttctaagc agattcatta caagatctta gaagaattga
      181 cagagcgtca atctataaaa gcaattggta aacattgcga tgtttctgtg acaactgttc
      241 agcggacatt gtccgccttg tcatcagata tccgtcagaa tcttgagtat ttacctcggt
      301 gtctattatt cgatgagttt cgttctcttt ctacatggca cggtaaattt tctttttctt
      361 gtatggacgg tgagaccggg aaactgtttg agatcttacc tagccgccgt aaaaaagatc
      421 tcgttgcttt tttcatgcgt ttttctctca aagcgaggtt gggtgtacat tacatcgtaa
      481 cggatatgaa cgctccttat ttctcactag ttaaagagtg ttttccaaat gcacaagtga
      541 tcgtcgatcg gtttcacatt gttcaacatc tgaaccgctt cttcgatgcg gttcgaaaac
      601 gtgtgatgaa aacacttgat caaaaagacc caatacaagc taaacagtat cgccaattga
      661 aatccttatt caaattactt ttaaaatcag aggatcgttt agattacaat acactaacga
      721 aaagacgcaa tttcaattgg agactattga ctgaaacaga agtagttgac cgccttctaa
      781 agatttctcc tgaacttaaa acagcttatc gttactacca agacctgctt actgcgtatc
      841 aggaaaaaga tccagatact tttttccagc tgcttcgagc aatgcctgga gaagttccat
      901 tggaactgca ccatatcaaa aaagcctttt tcaaatacga gaaagggatt cggctggcat
      961 tactgaagaa gtattcgaat gcacgactgg aaaatctcca tacgggttct gttgcaaagt
     1021 tttaaatcta ctatcaaata aggtagaata atagaaaaag atagc
//
LOCUS       DAYYAO010000212         1060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000212 DAYYAO010000000
VERSION     DAYYAO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.221"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..994)
                     /locus_tag="ACHARI_002945"
     CDS             complement(38..994)
                     /locus_tag="ACHARI_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443887.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DAYYAO010000213         1045 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000213 DAYYAO010000000
VERSION     DAYYAO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.222"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHARI_002946"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHARI_002946"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8443888.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DAYYAO010000214         1035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000214 DAYYAO010000000
VERSION     DAYYAO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.223"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..1035
                     /locus_tag="ACHARI_002947"
                     /pseudo
     CDS             414..1035
                     /locus_tag="ACHARI_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          383 a          178 c          185 g          289 t
ORIGIN      
        1 ttatttatca cggatgagct tgaaatcgtc ttttatacgt atcaaattga aatgttcggc
       61 atacaaccta tcgttgttac tacattacta attgtcactt gttaaggaac ttggatcctt
      121 tctaaacatt ccctagagat aaattttact ttatccctcc gttaatatcc aatttaagca
      181 cctttccgat tcagggttaa tttgcatatc aagtgcagct tgtgttctag taagattagc
      241 ccttttagtc cggctgagtt gacttatgga tataagatat ggtcatagca aatagatact
      301 acgtatagtc tgttaattta gtgtcattaa tcttaagttg gataggctac aattaactag
      361 acagaaaaat taaggtgtgt agactagaag aaaacatacc aggaggaatt tttatgtcta
      421 agagaacacg aagaactttt tcacaagaat tcaagcaaca aatcgtcaat ctttacttag
      481 ctggaaagcc acgtgtagaa atcattcgag aatatgaact aacggcttca gcatttgaca
      541 aatgggtaaa gcaatctaaa acgagtggtt cattcaaaga aaaagataat cctacgcctg
      601 aacaaaaaga attgttagaa ctacgtaaaa gaaaccagca attagaaatg gaaaatgata
      661 ttttaaagca agcagcgctg atattcggac gaagagacaa gtaatcgatg cgaataagca
      721 tctttaccct atatcagcga tgtgcagaat attaggtcta tcacgtcagt cctattatta
      781 tcaatcaaaa ccaaagaaag acgaatcaga acttgaagaa gcagtcgctg aagaatttat
      841 ccgcagccga aaggcccacg gctcaagaaa aataaaaaaa gccttatcaa aacgaggcat
      901 tcagatcagc cgacgaaaaa ttagtagaat catgaaaaat agaggattaa aatcgagcta
      961 tactgttgct tattttaaag tacatcattc tacttgcaat gaagccaaaa cgacaaacgt
     1021 attgaatcgt aaatt
//
LOCUS       DAYYAO010000215         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000215 DAYYAO010000000
VERSION     DAYYAO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.224"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..705
                     /locus_tag="ACHARI_002948"
     CDS             <1..705
                     /locus_tag="ACHARI_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8443889.1"
                     /translation="DADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKV
                     DVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPN
                     AEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSN
                     EKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILL
                     GTLGLLGYKNRKKENE"
     gene            839..>1001
                     /locus_tag="ACHARI_002949"
     CDS             839..>1001
                     /locus_tag="ACHARI_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8443890.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVS"
BASE COUNT          400 a          131 c          205 g          265 t
ORIGIN      
        1 gatgccgata aatacaatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca
       61 gacgcatctg agggaattaa gaacaaagaa gatctaccaa aaggaactac ttatacttgg
      121 aaagagaaag tagatgttag tacagctgga aacaaaaaag ggattgtagt agtaacatat
      181 ccagatggat ctaaagaaga agtagaggtt actatttctg tagaagataa aaaagcaccg
      241 aataaacctc aagttgatcc tattacagag ggtgaccaaa ttgttactgg taaaactgaa
      301 ccaaatgcag aggtgacagt tacattacca aacgggagtc aataccatgg gacagctgat
      361 aaaaatggta attttacagt taaagttcct aaattagagg caggaacaaa agtgatagta
      421 actgcaactg atgaatctgg taatactagt gaacctacta atgtagttgt ttcttcaaac
      481 gaaaaagaca gtgaaaaagc cgttagtaaa gataataaga ctgacaatca aggtagcaag
      541 caaaacaaaa atagaggcaa aagcagtcct caaaagcagt cctcaaaagc ttatcctaag
      601 acgggagaga ttgatagtaa tatctttact attagtggtg gtttaatcct gttaggaact
      661 ttagggttat tagggtataa aaatcgaaaa aaagagaatg aatagaaaat caatttctta
      721 aatcttctct gaaaagagga agaatctttt tcttcctctt tttgtttttt attttgggag
      781 ataggttgtt agttaaaaat gaagatactt ttcatagaag ggaaaagaat gtgattaagt
      841 gaaaaaaata gtaattattg gatcaaacca ttcggggcta gctgcagctc gatttctaaa
      901 cttatgtgat tcaaattatg aagttattct gatagataaa aaatcagaaa ttagttactt
      961 agaatgtggg acatcgttgt tcataaaaag agaagttagc a
//
LOCUS       DAYYAO010000216         1000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000216 DAYYAO010000000
VERSION     DAYYAO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.225"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..636)
                     /locus_tag="ACHARI_002950"
     CDS             complement(82..636)
                     /locus_tag="ACHARI_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443891.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          324 a          186 c          107 g          383 t
ORIGIN      
        1 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
       61 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      121 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      181 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      241 taatgtatgt gttcctatat catctcttcc taatttttct gctacatctg catatatctg
      301 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      361 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      421 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      481 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
      541 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
      601 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttcccttc ttaaaaatat
      661 tctgagttca tgattatata tatattttac cataatcatg aacttatata tatactctgt
      721 taaaagtgaa gaaaatcaag gggtttagtt tatgatttta tatcatttca gtaactcaga
      781 atattccgac ttttttgtac aaaaaaatta accttactta tatgaagatt ctaagctctt
      841 atttaaattt tagacgggca gttatccgta gaagttctta gaatagctaa tttacataat
      901 taaattatat gaaatttaag tttgaacaat atactaagaa ctccccttac tataaagtga
      961 aataaattag catatgaata accgtgctac caaacttttc
//
LOCUS       DAYYAO010000217         1000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000217 DAYYAO010000000
VERSION     DAYYAO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.226"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1000
                     /locus_tag="ACHARI_002951"
                     /pseudo
     CDS             369..1000
                     /locus_tag="ACHARI_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAYYAO010000218          993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000218 DAYYAO010000000
VERSION     DAYYAO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.227"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..235)
                     /locus_tag="ACHARI_002952"
     CDS             complement(<1..235)
                     /locus_tag="ACHARI_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443892.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNH"
     gene            complement(237..>993)
                     /locus_tag="ACHARI_002953"
     CDS             complement(237..>993)
                     /locus_tag="ACHARI_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8443893.1"
                     /translation="SSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSIL
                     LVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEI
                     SAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMH
                     NGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEM
                     VEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          375 a          174 c          119 g          325 t
ORIGIN      
        1 tatggttagt taacaagtta attttacgtt taaaggaaga accgattttc cggtgattca
       61 caaaaacaac atcattaaat agccctaatt cttttatacg ctcactatat cttctagcgt
      121 tttcaaattc agaataaata aataaatctc cttttgattc tcggtaaaaa ttttgataaa
      181 catttaatat attaatcaaa tgtaaaggag tccaagcaat gaaagctgtc ttcatattaa
      241 ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat aataccagaa aacaattgaa
      301 atgacatcgc gcttattgaa aagtacatga gcctccacca tttcatctat aaaaatagat
      361 agtataaaaa ttaaaacaat accaagaaac gcataattca ctttacttct aaaaaatgtt
      421 ttgagtaaac taaagatatt taacacatag aaagcataga tacatataaa agcaattata
      481 ccacttgata caagtacttg aagaaatcca ttatgcatgt ttccatgtgc tctttttagt
      541 tcattcacat ctgtagtatc aagatctact tttttcaaaa attttgcatc gctattccca
      601 tagaaatcgg tctctccata accaaataac aaagttggtc ttataacttt aagcgcagct
      661 ttccaaatcg taaaacgacc agctgaaatt tcggagttat cactagaatg ctgtaatatt
      721 acttcttttt ctggaagtgg agttttatct gattcatttg actctataac ttctactttt
      781 ctatcaatcg atacaggaat tgttgctaaa aaatccttag cataattagt accaaaagaa
      841 aaatatacca atagaactaa taatatgctt accatccatt tgatttttac aggtagtact
      901 tctctaatgc tctttctaaa ttttgaattg gcaaacaaca aaagaaataa tactaaaaaa
      961 gaacaaatta ctacctgggc acctctagac gaa
//
LOCUS       DAYYAO010000219          971 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000219 DAYYAO010000000
VERSION     DAYYAO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.228"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..793
                     /gene="pnuC"
                     /locus_tag="ACHARI_002954"
     CDS             44..793
                     /gene="pnuC"
                     /locus_tag="ACHARI_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8443894.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT          276 a          190 c          183 g          322 t
ORIGIN      
        1 atacagaaca cactacattt agtgtcttaa ggagtcctca tacatgaagt catattttca
       61 gtttttagct catcagctaa aaggctggcc gcaacaaaat tattatcttt tctttttcag
      121 tcttggctgc caggtcatga ctcttgtcaa tagtcccatc acttggctgt ccgttattac
      181 atttatcggt accacgcttg gtgtattgtg tattttatcc atcaatgcgg caaaatcagt
      241 caacggtatt ttaggtattt tgtctgcgat ttgttttatt atcgttgggt tttcagcaaa
      301 gaactattta agtatcggtg aacagattgc ttatatgatc acattggata tccccgtatt
      361 gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc cgaaaattca ctggaaaaag
      421 ctggtttatc gcaatcatca gtactttcct tgtctatctt gtttcaggat tcctgattgg
      481 tcgttataca gatgatcctc ggccgtgggt agatgccatt gctttttcta ttagcttgac
      541 tgctggtatc atcagtttct tgcgttacaa caaccaatat tactggtggt tagcttcagg
      601 gcttgctcaa atggtccttt ggtatatttc atttacgcat ggatctgcaa gtttggcgat
      661 gttcgtgaac agctctgttt acttgctgaa tgatgtcctt gcatttaccg tttcaccttg
      721 gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa gaagaatact acaacgcaca
      781 acaacacgca taaaaaaact gagcgtggga caaaagtaaa gaacactttt gtctcacgct
      841 ctaaacacgt ataaacggcg gaatcagaag caactccttc ggaaataagc tgaaattcac
      901 aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg
      961 tcccagcctc t
//
LOCUS       DAYYAO010000220          957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000220 DAYYAO010000000
VERSION     DAYYAO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.229"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          365 a          109 c          181 g          302 t
ORIGIN      
        1 tttcttggct acttttttat ttttatgtat ctacaaaagt ttccaggggc agaacctgtg
       61 attattaatg gaagaacctc aagtattgtc ttaaatcatt gttatgaaaa tggattaaaa
      121 gagttcatca aggtgtgatt aacaagttac ttttcattac atcgaatttt tcagaagttg
      181 cttatattgg tgatgatatt aatgatttat catgtatgac atctgtgaag aagcgggagg
      241 aatagtagaa tgtccaaaaa atgccacaag aaatgagatt gaattatctg attttattgt
      301 aactcacagt ggtggtgacg aatctgtgat agagtttatt gagtggatat tggaatcgat
      361 atagtgtagt agtacagaaa aattatcata caattaatac taggcataag aatattggca
      421 tattctagag ttaaaagtaa ctattcgaaa aggataaggt tagaattttt tgttttgcca
      481 gtggaagaat ataaaaaggg cattggaaat taatatatta gaatgctaag cggagaaaaa
      541 aatatatgaa aagtacgtcc ttgtgatggg atggaaaaat acacttattt caaacaacga
      601 taatataatt atgtgaaccg aagtatactc gcttctaatt caatcctcaa atcccgacag
      661 tcgcgacttc aaaaaagcgt atagttttaa tgtatcccca tatgatccga aaaatggtaa
      721 tttacaatta acagtttgat gatgttgaag atgtggaaat gctgaaatta ccatgatgag
      781 aaacacatta attaaaggga attaattaac tagaatataa tctgattcaa tttgaattaa
      841 gcaaaaaaag gctgagtttt aaataatcgc gaattgtaaa attaagttag aaaaataaaa
      901 aggcatttat ggtacactca aattgtattt ccgacgaaag aaaacaaagg agtgtaa
//
LOCUS       DAYYAO010000221          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000221 DAYYAO010000000
VERSION     DAYYAO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.230"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHARI_002955"
     rRNA            <1..>943
                     /locus_tag="ACHARI_002955"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYAO010000222          912 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000222 DAYYAO010000000
VERSION     DAYYAO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.231"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHARI_002956"
     CDS             136..309
                     /locus_tag="ACHARI_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443895.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          304 a          147 c          110 g          351 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gc
//
LOCUS       DAYYAO010000223          900 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000223 DAYYAO010000000
VERSION     DAYYAO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.232"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          348 a          103 c          113 g          336 t
ORIGIN      
        1 agcaatatat aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga
       61 gcaaatataa aatttaataa aaataagaac taaataggaa aacgtagacg taattttcta
      121 cgttttcctt ttcatatata ttaaatatta cggaaaatta tataaactaa tttagtgttt
      181 tgtttgtaat tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatt
      241 agacattcta tgctcatttt tctccaaatt tgtttcaact ctttcgtact taattttgcg
      301 tatttgaaaa ttcttctttt agagtacttt tattacgata ctagtatgat atatatctac
      361 agcaaacagt taaaatcttc ctaaaataag cgaatagcat tcctaaaacc tataaaattt
      421 ttagatgaag cgacaagtgg acaattaata aactacttga acatgaaatt atggatcacc
      481 tttcaaattt agaaaatgga attcttatac ttattgcgca acatttattg attgcaaaat
      541 tatgtgacaa aaagaactag tagaagaagg aataatggct tgataataaa gtactaccta
      601 ttaaaggtag tactttattt aatttagtat aaattaacat actactatat tttaggtttc
      661 atattatata aattaataat atactaccat ttgttggtag tataatttaa aggtaataag
      721 ttattctttt attactatgt ttgggtttta tagcaaagaa agtttaataa gagtttggac
      781 ctagcaaaca atattaatga ggtcattaaa tcttattatc tttagtgata taatgaaata
      841 tctacacgac aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat
//
LOCUS       DAYYAO010000224          859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000224 DAYYAO010000000
VERSION     DAYYAO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.233"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..>859
                     /locus_tag="ACHARI_002957"
     CDS             33..>859
                     /locus_tag="ACHARI_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443896.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     "
BASE COUNT          281 a          111 c          125 g          342 t
ORIGIN      
        1 aataaatgat actatatgta atataaaaaa acatgaggtg gtatatgaat ttgaaaaaaa
       61 ttttttctgt aaaaatattt atagtttatg catttgttag tttattatat ttatttccag
      121 catttaaaaa catggaaata tttggctttt atgatacggc atttcacatt aatcgtgcgt
      181 taagtttaga atctattttt agctccccta ttaattttga aacgtttaga tcatatggca
      241 tgcaagtaaa taatttttac ccatggttaa ctctatatcc tttattttta ttgatcaagt
      301 ttactaattt agcaataggc tataaccttt ttctttatat tgttacgctc attactctat
      361 ttatatgtca ttatgtaatg tatgaaatta ccaaaaaaca tgttactagc tcctttttcg
      421 ctattatata tacaacatct tcttttagga gtgtagaaat atttcttcga ggggcaatgg
      481 gggaattact cgcaatgtca atacttcctc ttattttact cgggtttata aagctttacg
      541 actcaaaaaa agaatcttgg gttatgttgg cgattagcat gactttatta atttacactc
      601 atgttctttc tgtagctatg actatggtaa tgattataat tgctttaatt attcaatttt
      661 atcggaaaaa gaaaatagaa atcttcttaa ttattaaatt aataaaagct gctatagtta
      721 ctttacttct ttcactagcg tttataggtc ctatgattga gcagacattg tatcaagatc
      781 tgaatagacc ttatgtggat atattacaaa aaagagcaat ttatctgggt aaagagtttt
      841 tgattggaag tatagattc
//
LOCUS       DAYYAO010000225          804 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000225 DAYYAO010000000
VERSION     DAYYAO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.234"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..693
                     /locus_tag="ACHARI_002958"
     CDS             <1..693
                     /locus_tag="ACHARI_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8443897.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          296 a          126 c          148 g          234 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct ccca
//
LOCUS       DAYYAO010000226          791 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000226 DAYYAO010000000
VERSION     DAYYAO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.235"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACHARI_002959"
     CDS             <1..716
                     /locus_tag="ACHARI_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8443898.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DAYYAO010000227          781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000227 DAYYAO010000000
VERSION     DAYYAO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.236"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>781)
                     /locus_tag="ACHARI_002960"
     CDS             complement(<1..>781)
                     /locus_tag="ACHARI_002960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8443899.1"
                     /translation="DVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYIGV
                     EIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGR
                     KKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKDIY
                     AIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFNYN
                     FYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRI"
BASE COUNT          256 a          136 c           96 g          293 t
ORIGIN      
        1 taattcttaa ttttacacga ctattttttc tcaaaaaaac aggtctaatg aaatcttcta
       61 tttctatata atctatagac tcacttaata atcgtgctgt ttttaaattc aaacctgctg
      121 cgtaaaaatt gtaattaaat atagaaaagc catttgtaaa ttgggaacca gaaggtgtaa
      181 aatcttgatt gtcaatatgt tgagttgcaa tataagctgt tcttagagca ttagaaatag
      241 aaaaatcaac actacttcta ttaatggacg taaaaaaagt tgtagcacaa tccccaatag
      301 catatatatc ttttatattt gtttgctggt attcattagt tagatatgct ccactaacat
      361 ctctcaagag ttcctttttg ccaattggac tattagatag aaatcctata gcttgtacga
      421 ccatatccat agagtattgg gtaccacttg tagtgactat tttttcaatc ttttttcttc
      481 ctaaatattt tttaggactt tctgatagac aaagatgtat tccatttttt gatagtattt
      541 cttcaacttt atctgaaaaa ctacggtcgt aataagtcga caacactcta tctgcaacat
      601 cgaaaagata tacttctttc cctctttttc ttatggcttc agcaatttca actccaatat
      661 atcctgctcc aataattcct acttttttaa tagtattatc atctaaagct ttaaaaatct
      721 ctttagcatt tggaaaattc tttatcgaaa aaatattagc taaattattc cccggtacat
      781 c
//
LOCUS       DAYYAO010000228          750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000228 DAYYAO010000000
VERSION     DAYYAO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.238"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            298..>750
                     /locus_tag="ACHARI_002961"
     CDS             298..>750
                     /locus_tag="ACHARI_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8443900.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT          252 a          140 c          117 g          241 t
ORIGIN      
        1 taaaaacacc agatttcaaa tttcacacaa tttaaactag atcaatcatc aaccttcttc
       61 aaattgttag aaccctttcc cgaggagctg cagcataagc aaaaaaacaa acaggaagcc
      121 aaatggctaa gacatttttt ctattagtct agttgcttct cgtcgcaagt ttaagacttt
      181 agaaagttat ttctaatgat gtttgggttg ttttgatcaa tgcttatgca cagctcctta
      241 cctattattt atctcaacag atctaacaaa ttaatggact atttaaggag tttttttatg
      301 cttacgatct attcttctcg cacacatagt taccatctga tggcaaactg gctgaaagac
      361 catcaaatgc cttacagaga acgaattttc acacctaaac agcctttaac aaggaaagag
      421 atattttatt tattgtcatt aacagaaaac ggatttgacg atctattagc taaacgctcg
      481 attgattata aaaatttgaa agaccaaatc gaccaatgta cgacctcaga gcttgtcgat
      541 ctgatcgtct cgcattttac tttgttacaa cgtcccatca ttactgacgg aaagaaatta
      601 ttagtcggat tcaatgaaga tagcatgcgt ctttttatcc ctgtacaatg gcgcagaagt
      661 caactgaaat ctgtctataa cgattttaca tgggtaagca atctagaaaa ttctacggat
      721 ttagatgatg aatttttaaa ttattatata
//
LOCUS       DAYYAO010000229          744 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000229 DAYYAO010000000
VERSION     DAYYAO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.239"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..381
                     /locus_tag="ACHARI_002962"
     CDS             178..381
                     /locus_tag="ACHARI_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFM8443901.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="ACHARI_002963"
     CDS             381..731
                     /locus_tag="ACHARI_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8443902.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          300 a           80 c          120 g          244 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta tact
//
LOCUS       DAYYAO010000230          678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000230 DAYYAO010000000
VERSION     DAYYAO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.240"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..222
                     /locus_tag="ACHARI_002964"
     CDS             <1..222
                     /locus_tag="ACHARI_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8443903.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            403..>678
                     /locus_tag="ACHARI_002965"
     CDS             403..>678
                     /locus_tag="ACHARI_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443904.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          255 a          125 c          118 g          180 t
ORIGIN      
        1 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
       61 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      121 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      181 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
      241 gattttattc tttatttaaa actttgcaac agaaccaccc gcactatgcc attacattta
      301 tatctacgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
      361 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
      421 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
      481 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
      541 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
      601 agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa agtcactctt
      661 ggttggacgc ctgacgtc
//
LOCUS       DAYYAO010000231          654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000231 DAYYAO010000000
VERSION     DAYYAO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.241"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..602)
                     /locus_tag="ACHARI_002966"
                     /pseudo
     CDS             complement(1..602)
                     /locus_tag="ACHARI_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          184 a          120 c           87 g          263 t
ORIGIN      
        1 atctaatttt tttacccagt aataataagt tgacttcgct aattgtgcaa ttgaaagtaa
       61 tagattcaat ttaaattctt ttttgagctg aaggattgtt ttaaccttga tttcttcttg
      121 ctcaaatctt gttcttgaat caaggtttct aactttttta aatatgcgtt ctctgctctt
      181 aaacgaataa cttcttcttc aagagactca tctttaagtt ttttaggaat gtttagcttg
      241 gaattcatac taatttttct gccccttttt tggctctcaa gtgaagaagc accgccttct
      301 tcatattgct ctatccattt acttagagtt ctatttgaac cgatattaaa ttttttagca
      361 gtttcttgga tagaaagacc atttgtttcc atatattcta taacatcaag tttaaatttt
      421 gtagtgtagc ttttgccacc tccaaccaag ccttcccaac catgatagtt ataaatcctt
      481 acccaatgtc taaccagtgt acgatttatt tgatatttat gtgcaagata tttgtagccg
      541 ccttcgttat ttaaatagtc tgaaactact tttttcttaa aaacaaatgt atatttccgc
      601 aaaaaaagca ccccttttaa ttagatttct agtctaactt ttggggtgca catc
//
LOCUS       DAYYAO010000232          649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000232 DAYYAO010000000
VERSION     DAYYAO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.242"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>649
                     /locus_tag="ACHARI_002967"
     CDS             <1..>649
                     /locus_tag="ACHARI_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8443905.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAYYAO010000233          621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000233 DAYYAO010000000
VERSION     DAYYAO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.243"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          229 a           71 c          139 g          182 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc t
//
LOCUS       DAYYAO010000234          594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000234 DAYYAO010000000
VERSION     DAYYAO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.244"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACHARI_002968"
     CDS             <1..572
                     /locus_tag="ACHARI_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8443906.1"
                     /translation="IRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIK
                     GRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQY
                     QMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLN
                     EYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          251 a           75 c           99 g          169 t
ORIGIN      
        1 aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg
       61 tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg
      121 aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag
      181 aaataattag ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc
      241 aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat
      301 ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga gaataacatt attcaaagca
      361 tgtctagaaa aggaaattgc ttagataatt ctgtaataga aaattttttt ggtgtgctta
      421 aatcagagtt tttttatcga gaaaaattcc gatcaattga gatatttcaa agtaaattaa
      481 atgagtatat taggtggtat aataacaaaa ggataaaact aaagttaaat ggactatctc
      541 cagtagaata tcggaaacag tccattaaat agttcaactt ttggggttca gttc
//
LOCUS       DAYYAO010000235          575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000235 DAYYAO010000000
VERSION     DAYYAO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.245"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          186 a           81 c           81 g          227 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct tttta
//
LOCUS       DAYYAO010000236          557 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000236 DAYYAO010000000
VERSION     DAYYAO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.246"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>557
                     /locus_tag="ACHARI_002969"
     CDS             <1..>557
                     /locus_tag="ACHARI_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8443907.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCG"
BASE COUNT          205 a           88 c           97 g          167 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtggaca
//
LOCUS       DAYYAO010000237          516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000237 DAYYAO010000000
VERSION     DAYYAO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.248"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>516)
                     /locus_tag="ACHARI_002970"
     CDS             complement(107..>516)
                     /locus_tag="ACHARI_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8443908.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          172 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccat
//
LOCUS       DAYYAO010000238          512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000238 DAYYAO010000000
VERSION     DAYYAO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.249"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHARI_002971"
     CDS             complement(<1..132)
                     /locus_tag="ACHARI_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8443909.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          184 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag ac
//
LOCUS       DAYYAO010000239          497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000239 DAYYAO010000000
VERSION     DAYYAO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.250"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>497
                     /locus_tag="ACHARI_002972"
     rRNA            <1..>497
                     /locus_tag="ACHARI_002972"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          120 c          147 g          111 t
ORIGIN      
        1 ctggtctgta actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct
       61 ggtagtccac gccgtaaacg atgagtgcta agtgttggag ggtttccgcc cttcagtgct
      121 gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg
      181 aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga
      241 accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc
      301 aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc
      361 cgcaacgagc gcaaccctta ttgttagttg ccatcattca gttgggcact ctagcaagac
      421 tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
      481 tgggctacac acgtgct
//
LOCUS       DAYYAO010000240          495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000240 DAYYAO010000000
VERSION     DAYYAO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.251"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>495)
                     /locus_tag="ACHARI_002973"
     rRNA            complement(<1..>495)
                     /locus_tag="ACHARI_002973"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           99 a          162 c          106 g          128 t
ORIGIN      
        1 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
       61 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      121 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      181 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      241 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      301 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      361 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      421 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
      481 cccgaaagcg ccttt
//
LOCUS       DAYYAO010000241          493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000241 DAYYAO010000000
VERSION     DAYYAO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.252"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..>493)
                     /locus_tag="ACHARI_002974"
     CDS             complement(54..>493)
                     /locus_tag="ACHARI_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8443910.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          133 a           92 c          115 g          153 t
ORIGIN      
        1 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
       61 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
      121 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
      181 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
      241 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
      301 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
      361 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
      421 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
      481 tcggcttttt tga
//
LOCUS       DAYYAO010000242          470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000242 DAYYAO010000000
VERSION     DAYYAO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.253"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(200..400)
                     /locus_tag="ACHARI_002975"
     CDS             complement(200..400)
                     /locus_tag="ACHARI_002975"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443911.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
BASE COUNT          123 a           92 c           51 g          204 t
ORIGIN      
        1 tcttttcctt ttagaaatgg tctttctaat ccggtatcaa ttcttgcttg atcaatagcc
       61 tcttttgtca tttcaaaaac ataatttttc aattgattcg tttgttgttt atctaacatt
      121 actgtaattc ctgcatccat tatttttctc ctctcatttt ctatttcttc aatcagccaa
      181 ctcgccaagt tgtatttatt tagattttca aatcattaag taaccaattt accaactttt
      241 gataagtaga tgctctaata ctttctttgc tcccattttc gattttgaca tatgttttcc
      301 gatttatacc tatcaatttt gctgcatcgt ttttagtaag tataagatcg gctctatgtc
      361 ttcttaagct tctaatgaat tgatcattaa gagttatcat tcagcgcctc ctttcaaatt
      421 attcactttg tggtttttat cttaataagc acattttaaa ccacaaaacg
//
LOCUS       DAYYAO010000243          448 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000243 DAYYAO010000000
VERSION     DAYYAO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.254"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACHARI_002976"
     CDS             complement(<1..210)
                     /locus_tag="ACHARI_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8443912.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT          142 a           80 c           55 g          171 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttac
//
LOCUS       DAYYAO010000244          426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000244 DAYYAO010000000
VERSION     DAYYAO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.257"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>426
                     /locus_tag="ACHARI_002977"
     CDS             <1..>426
                     /locus_tag="ACHARI_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8443913.1"
                     /translation="HLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKT
                     EYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYG
                     HHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          156 a           78 c           71 g          121 t
ORIGIN      
        1 gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt ttttagatta
       61 cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt cttactttcg
      121 atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc gtaatggtga
      181 gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg aaaccactat
      241 tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga tcgaaaaaat
      301 gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc tgactgaaga
      361 agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt ttatggacgc
      421 tactta
//
LOCUS       DAYYAO010000245          408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000245 DAYYAO010000000
VERSION     DAYYAO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.258"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACHARI_002978"
     CDS             <1..390
                     /locus_tag="ACHARI_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8443914.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           73 c           64 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggag
//
LOCUS       DAYYAO010000246          386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000246 DAYYAO010000000
VERSION     DAYYAO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.261"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACHARI_002979"
     CDS             <1..>386
                     /locus_tag="ACHARI_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8443915.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKR
                     LVKQFDEPKVVVTDKAPSITSAFKKL"
BASE COUNT          133 a           64 c           76 g          113 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt
      301 aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agccccctct
      361 attacaagtg cctttaagaa actaaa
//
LOCUS       DAYYAO010000247          367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000247 DAYYAO010000000
VERSION     DAYYAO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.262"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>367
                     /locus_tag="ACHARI_002980"
     CDS             <1..>367
                     /locus_tag="ACHARI_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8443916.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGER"
BASE COUNT          137 a           61 c           80 g           89 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgag
//
LOCUS       DAYYAO010000248          350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000248 DAYYAO010000000
VERSION     DAYYAO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.263"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>350
                     /locus_tag="ACHARI_002981"
     CDS             82..>350
                     /locus_tag="ACHARI_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8443917.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQR"
BASE COUNT          124 a           55 c           73 g           98 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta
//
LOCUS       DAYYAO010000249          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000249 DAYYAO010000000
VERSION     DAYYAO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.265"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHARI_002982"
     CDS             complement(<1..>321)
                     /locus_tag="ACHARI_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8443918.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYAO010000250          310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000250 DAYYAO010000000
VERSION     DAYYAO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.denovo.266"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>310
                     /locus_tag="ACHARI_002983"
     CDS             <1..>310
                     /locus_tag="ACHARI_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8443919.1"
                     /translation="DTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     L"
BASE COUNT           97 a           63 c           53 g           97 t
ORIGIN      
        1 tgatacgatc gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc
       61 taaagtcatt atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct
      121 taatcaatgg ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg
      181 aaaagaattt tctaactgga aagccattag taaccaacat gatattgata tatattttgc
      241 ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg
      301 taatggactg
//
LOCUS       DAYYAO010000251        45674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.guided.011, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000251 DAYYAO010000000
VERSION     DAYYAO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.guided.011"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..843)
                     /locus_tag="ACHARI_002984"
     CDS             complement(124..843)
                     /locus_tag="ACHARI_002984"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8443920.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(892..2538)
                     /locus_tag="ACHARI_002985"
     CDS             complement(892..2538)
                     /locus_tag="ACHARI_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8443921.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2717..3307
                     /locus_tag="ACHARI_002986"
     CDS             2717..3307
                     /locus_tag="ACHARI_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8443922.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3399..4904
                     /locus_tag="ACHARI_002987"
     CDS             3399..4904
                     /locus_tag="ACHARI_002987"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8443923.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(4988..6340)
                     /gene="celB"
                     /locus_tag="ACHARI_002988"
     CDS             complement(4988..6340)
                     /gene="celB"
                     /locus_tag="ACHARI_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8443924.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6340..6858)
                     /locus_tag="ACHARI_002989"
     CDS             complement(6340..6858)
                     /locus_tag="ACHARI_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443925.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(6874..7200)
                     /locus_tag="ACHARI_002990"
     CDS             complement(6874..7200)
                     /locus_tag="ACHARI_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8443926.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7213..9192)
                     /locus_tag="ACHARI_002991"
     CDS             complement(7213..9192)
                     /locus_tag="ACHARI_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8443927.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9213..9527)
                     /locus_tag="ACHARI_002992"
     CDS             complement(9213..9527)
                     /locus_tag="ACHARI_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8443928.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9695..9988
                     /locus_tag="ACHARI_002993"
     CDS             9695..9988
                     /locus_tag="ACHARI_002993"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8443929.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10066..11178)
                     /locus_tag="ACHARI_002994"
     CDS             complement(10066..11178)
                     /locus_tag="ACHARI_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8443930.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11749..12714
                     /locus_tag="ACHARI_002995"
     CDS             11749..12714
                     /locus_tag="ACHARI_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8443931.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            12736..12948
                     /locus_tag="ACHARI_002996"
     CDS             12736..12948
                     /locus_tag="ACHARI_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8443932.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            13023..13499
                     /locus_tag="ACHARI_002997"
     CDS             13023..13499
                     /locus_tag="ACHARI_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8443933.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            13496..15304
                     /locus_tag="ACHARI_002998"
     CDS             13496..15304
                     /locus_tag="ACHARI_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8443934.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            15381..15596
                     /locus_tag="ACHARI_002999"
     CDS             15381..15596
                     /locus_tag="ACHARI_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8443935.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            15688..16551
                     /locus_tag="ACHARI_003000"
     CDS             15688..16551
                     /locus_tag="ACHARI_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8443936.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            16588..17124
                     /locus_tag="ACHARI_003001"
     CDS             16588..17124
                     /locus_tag="ACHARI_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8443937.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            17138..17560
                     /locus_tag="ACHARI_003002"
     CDS             17138..17560
                     /locus_tag="ACHARI_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8443938.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            17490..19889
                     /locus_tag="ACHARI_003003"
     CDS             17490..19889
                     /locus_tag="ACHARI_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8443939.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            19921..21912
                     /locus_tag="ACHARI_003004"
     CDS             19921..21912
                     /locus_tag="ACHARI_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8443940.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            21935..22186
                     /locus_tag="ACHARI_003005"
     CDS             21935..22186
                     /locus_tag="ACHARI_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8443941.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            22176..23405
                     /locus_tag="ACHARI_003006"
     CDS             22176..23405
                     /locus_tag="ACHARI_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8443942.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            23402..25483
                     /locus_tag="ACHARI_003007"
     CDS             23402..25483
                     /locus_tag="ACHARI_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8443943.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            25632..29552
                     /locus_tag="ACHARI_003008"
     CDS             25632..29552
                     /locus_tag="ACHARI_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8443944.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            29553..30497
                     /locus_tag="ACHARI_003009"
     CDS             29553..30497
                     /locus_tag="ACHARI_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443945.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            30501..30704
                     /locus_tag="ACHARI_003010"
     CDS             30501..30704
                     /locus_tag="ACHARI_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8443946.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(31004..31240)
                     /locus_tag="ACHARI_003011"
     CDS             complement(31004..31240)
                     /locus_tag="ACHARI_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443947.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(31423..31794)
                     /locus_tag="ACHARI_003012"
     CDS             complement(31423..31794)
                     /locus_tag="ACHARI_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8443948.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(31791..33119)
                     /locus_tag="ACHARI_003013"
     CDS             complement(31791..33119)
                     /locus_tag="ACHARI_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8443949.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(33080..33409)
                     /gene="mobC"
                     /locus_tag="ACHARI_003014"
     CDS             complement(33080..33409)
                     /gene="mobC"
                     /locus_tag="ACHARI_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8443950.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(33667..34038)
                     /locus_tag="ACHARI_003015"
     CDS             complement(33667..34038)
                     /locus_tag="ACHARI_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8443951.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            34183..34368
                     /locus_tag="ACHARI_003016"
     CDS             34183..34368
                     /locus_tag="ACHARI_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8443952.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            34526..34693
                     /locus_tag="ACHARI_003017"
     CDS             34526..34693
                     /locus_tag="ACHARI_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443953.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            35311..35970
                     /gene="vanR-B"
                     /locus_tag="ACHARI_003018"
     CDS             35311..35970
                     /gene="vanR-B"
                     /locus_tag="ACHARI_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8443954.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            35973..37316
                     /gene="vanS-B"
                     /locus_tag="ACHARI_003019"
     CDS             35973..37316
                     /gene="vanS-B"
                     /locus_tag="ACHARI_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8443955.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            37487..38293
                     /locus_tag="ACHARI_003020"
     CDS             37487..38293
                     /locus_tag="ACHARI_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8443956.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            38311..39138
                     /gene="vanW-B"
                     /locus_tag="ACHARI_003021"
     CDS             38311..39138
                     /gene="vanW-B"
                     /locus_tag="ACHARI_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM8443957.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            39135..40106
                     /locus_tag="ACHARI_003022"
     CDS             39135..40106
                     /locus_tag="ACHARI_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8443958.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            40099..41127
                     /gene="vanB"
                     /locus_tag="ACHARI_003023"
     CDS             40099..41127
                     /gene="vanB"
                     /locus_tag="ACHARI_003023"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8443959.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            41133..41741
                     /locus_tag="ACHARI_003024"
     CDS             41133..41741
                     /locus_tag="ACHARI_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8443960.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(41807..41980)
                     /locus_tag="ACHARI_003025"
     CDS             complement(41807..41980)
                     /locus_tag="ACHARI_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443961.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            42329..42760
                     /locus_tag="ACHARI_003026"
     CDS             42329..42760
                     /locus_tag="ACHARI_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8443962.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            42767..43000
                     /locus_tag="ACHARI_003027"
     CDS             42767..43000
                     /locus_tag="ACHARI_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443963.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            43424..43624
                     /locus_tag="ACHARI_003028"
     CDS             43424..43624
                     /locus_tag="ACHARI_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8443964.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            43708..44901
                     /locus_tag="ACHARI_003029"
     CDS             43708..44901
                     /locus_tag="ACHARI_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443965.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            45148..45639
                     /locus_tag="ACHARI_003030"
                     /pseudo
     CDS             45148..45639
                     /locus_tag="ACHARI_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT        12357 a        11045 c        11234 g        11038 t
ORIGIN      
        1 gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa agccatccga aagtcagacg
       61 gacaagtctg cttttcagat ggcgctacaa ctactctgct tattctttta gctatccatt
      121 caattatttt tctctcactt gttctagctc ttctttggtc atgggtcgat attgcccttt
      181 attcaaatct ttaggcagcc aaaattttcc catacggatt ctttttaaat ataagacttc
      241 ttttcctact gctttgacca ttcgcttaac ttggtgaaac tttccttcat gcaaaatcag
      301 acggataatt gaagtttctg tctcttgatc tacttgatca atttggagtt ttgccggcaa
      361 tgtctgctca tcgccatcaa taatcagtcc ttctgcaaat aaacggatat cttcttctgt
      421 cattataccg ctaacctcag ctagatattc tttctccaca tgttttttag gtgatagtaa
      481 ctgatgggca aacaccccgt cattcgttaa aatcaagagg ccctctgtgt ctttatctaa
      541 tcggccgact ggaaaaagat cttcccgata atcctggtct tccaataaat cgattacggt
      601 ttgatcatga ttatcctgag tagcagaaat gactcctgca ggtttatgca aaatgtagta
      661 aaaatctttt tgatacgtaa tgatttctcc taaataagta accgcatctt cgaattcatt
      721 tacttgcgct ttatcgcttt tcaccacttg tccattgact tcgatcgttc cttttttgat
      781 taatcctttg acttctttgc ggctgccaag tcccatatct gctaaaaact tatctaaacg
      841 catgcgtttc ccccatttca aaagagcatc aatctagctc ttctgattca cttattttat
      901 ttttaattta tgtcgtaaac gcgctattcc tggtcctagt agtctttctg ccaagcggat
      961 tttcaaagct aaataaccat acactaaagc ccctatacct gctactacaa gaatcaacaa
     1021 tagagactgg aatttgctat cttcgtttaa aaaagcacca aaaatcatct ttgtgatcca
     1081 cgaagccaaa agcataatca aggtaagaat aaagatcagc aatgtcctgc gccagacaaa
     1141 acttggttta aagcgggcta cttcatggat tctctttaag atcaaggtac aagacacagc
     1201 aaatccgatc attgtcgcca ataaagggcc gtatacttca aataaacgaa tggctgggta
     1261 ttgaagaagg agtttcacta gaaacccaat aaccaaataa ttaattgccg ccttgttttc
     1321 aaacattcct tgcagcatgt tggaaactag catatatagt cctaggaaga gtcctacaaa
     1381 acttgcttgg atcagtacat tacttcctag agaatccggt gtataaaata atgtatttag
     1441 cggataggca agtaacataa caccaaatgt cgccggaaac ataaaaaatg aaaacaactg
     1501 taaatttccg cttgtcaatt ttgccagacc tactcgatct ttcaacgtaa cagcttctgt
     1561 aatcaacggt aacccagtag acgcgatcga tgttgccaat gcaataacta ccatcgtcaa
     1621 cttatctgga ttggcactaa aaatcgaaaa aagatccagt aactgtgcat tggaatactc
     1681 ggtggatgca gacatgatct tcataaaagt gaactgatcg actaatttga agatcgttac
     1741 ccctgaaccc acaataataa acggaatcgc ttctttaacg gtatctaaca acaattcctt
     1801 cgttgcaata tttattttgt tttcgctgta gtgaataaac gccgatgtat acgcttgatg
     1861 tttcttcaaa aaatacaaaa ggacaagaat acttgctagc ataccgataa atgccgcgaa
     1921 agttgattgc gtgaccgccg tcacatagtc accatccatg actttcatga tgatgaatgt
     1981 agccaatagc atataaaaaa tacgtgccac ctgttctaca atctgcgaca gcgcataagg
     2041 catcatttct tgattgcctt gcaaataccc tcgtatcaca ctcatacaag gaaaaaccaa
     2101 tacagcgata ctcaaagaac gcatcgttgg aatcagttct tctccgccac cagaagcatg
     2161 agctagccaa ggagaggcga tgtacatgaa caaagcaaac aacaggccta acccgcccat
     2221 catctgtaat gcacggataa ataatcgtcg tgaagtgcca tattcattca atgaattata
     2281 gcgtgcagtt tgttttgcaa tcgcagcagg tattcctgct gtagagatca gtagaaacaa
     2341 tgcatatata ttgtatccca tattgaataa tccattggca gccttagcat tttcgcccat
     2401 ccaagcatac cagggaatga tatagacagc gcctagcaat cttgaaatga tattactggc
     2461 ggtcatccaa gcagagcctc gtgccatttt ttcttgcggt gttaattgat ctaatgctgg
     2521 ttttctttga ttatccatcg gtcagattcc aactttctac aaaacattac tatcatttta
     2581 attggtaaaa cagaaactgg caatcgattt tatataactt ttataagaac aattgttttt
     2641 ttgaattcgg tttcgttttt ttggtaaagt aaagaaatat acagcctttt atggctaaca
     2701 tttataggag atatcaatga cacaagaaat aaaaaaacta cccgttatat ttttaggtct
     2761 tttctttatc acagttggat tgaattggtt cttgctccct cacgatatag cttccgccgg
     2821 agtgggcgcc atcggacatt tagttgaaac ggccttttct atccaacgct cagttactgt
     2881 ttggagcatc aatcttatga tgctgcttct agccgctgtc ctattaggaa aatcagtatt
     2941 ttttaagact gtagcgggaa gtctgctttt tcctgttgtt ttagagattg ttccaaaagt
     3001 ggaaatgatc tcctcacatt tcttttcact gatttcagga agtcttttgt tcagtcttgg
     3061 ggtttacaca ttgtacacag tcggggcctc aaatggcggt ataacgatac cgccgattat
     3121 tttccacaaa ttttttcggc ttccaatgcc gcaaggactt ttacttacca atagtcttat
     3181 cgtctttttg aattattatg tttttggatt gttggagaca ctattcgttc tcctatctat
     3241 tacagtcagt tcattgttta tgaaaatact catccgcttg tcccctgtgt caagaaaatt
     3301 tgaataaagc tggattttgt catagcggaa acaaacgata tattaaaaag agtgtgctat
     3361 aataaaccaa gaaattgaat aaagcgaggg atcaatcaat gagcttctct ttagaagaca
     3421 tccgtcattt actattaaaa gaaaatctat taaaagaatt tgtttcaaca agaggctggc
     3481 atcttgatgt accagaaaaa gctgaatttc ttcagttaag ttatgattca agaaaagctg
     3541 atgcttctac tttgtttttc tgtaaaggaa tgaatttcaa agaagaatat ttggattcag
     3601 caatcgaaca gggtattcaa tattatgtat cagaacaacc atacgaaaat tcagcagtag
     3661 gaatcatcgt aacggatatc cgtaaagcaa tggcgcttct tgctatggct ttttacgact
     3721 atccgcaaaa taaattgaaa gtgatcggat tcactggaac aaaaggtaaa acaactgctg
     3781 cgtactttac aaaagcaatc ttggatcaca caacaaataa aaaaacagca ttgctttcta
     3841 ctatgaatac aacgttagat ggcaaaacat atttcaaatc ccatttgaca acgcctgagt
     3901 cattagattt atatcgtatg atgtcagaag cagttgaaaa tggtatgact catctagtga
     3961 tggaagtttc ttcacaagct tacaaaacac aacgcgtata cggcttgact ttagatgtcg
     4021 gaatcttctt gaatatctcg cctgaccata tcagtccgat cgaacatccg acatttgatg
     4081 actatttcta ttgcaagcga caattgatct tgaattcaaa aacagttgtt ttaaatcatg
     4141 aaagtgacta ttttgattta cttagggaaa cagccgatct attccagatt ccaacgatca
     4201 cttatggtcg ttcggaagaa agcaattatc aagtgatccg ttcagataaa ggtactcacg
     4261 gttttacttt atcttctcat gaagatcgct tagctatctg cgatactgct tatgatattt
     4321 tgctggctgg aggattcaac caagaaaatg cagccagtgc gatcattgcc gcagcactaa
     4381 caggtgcctc cagtcaagat gcacaagacg gattaaaaga agctcgtgta ccaggccgaa
     4441 tggatcaatt gatccaatcc aatggagcgc atgtctatgt ggactatgca cataattatc
     4501 taagcttgaa aacattgctg aaatttgcca aaaatgaaca tccagatggc cgtgtaattg
     4561 ttgtattagg cagcccagga aacaaagcaa tctcccgtcg acatgatttt ggaaaagtgc
     4621 tatcagaaac agctgacatc gcctttttga cggcagatga cccagcattt gaagatccga
     4681 aaaaaatcgc ggaagaaatc aatgaagcaa ttactaatcc agatttgatc gttcattatg
     4741 aaatggatcg tcctgaagcg attcgacgcg ctttagcaga aagcgctccg caagattcag
     4801 tcgttattgc gggaaaaggc gtggatccct atcaaaaaat caatggtgta gatgaacctt
     4861 atgaagggga ttatgcaatc gtgaagcggt tgattgaaga ataatggtat ttatatacta
     4921 gtggtttccc atttctaaga aaaaaaggga ctgtgatttt ttgtcacagt tccttttttc
     4981 ttagaaatca ttttaacttt tcactagcta attctgctgc cttttctctt tcttcattta
     5041 ataatttatt atcatagaat ttgataaatg ggaaccagat aatgaatgcg atgattgcac
     5101 aaatgactgc taacacagca gctttccaat caccaccact accaacaaat gctccgatcc
     5161 cgactggtga aggccacggc atctgtgcaa gcatcggttt gacgatttcc agccgaatgg
     5221 caaaataagc gagtgtagca gaagccatcg gagctaagaa aaacggaacc gctaaatagg
     5281 gattgtagac gattggcatc ccgaaaatga ttggttcatt gatattgaat aatcctggta
     5341 caagtgaggc ttttcctaac actttcagct gatcagattt cgcgaggaat gcaataaaga
     5401 tgatcaatcc taaagtcgca cctgatccac caacggttac ataagaattg ttgaattctc
     5461 ctgccaacgg ataatttgcg ccttgtgcgt tagctgccat attagctaat acgatcgggg
     5521 tcaagaaaga agtgatgata ttcgctccat ggatccctac gatccacaat gcatggatca
     5581 agaaataaat caccatgaca ccaagccatg tattcgtcaa ctgcgtaaca aacccaaatg
     5641 ggatagcaat cattttgaag atatccgttc ctagtgcaac taataatcca ttgatgatca
     5701 tcaccacaaa cgcaatgaca aatgttggga tcagagcagt gaaagaacga gaaactcctg
     5761 gcggaacaac atctggcatt tttatgaccc aatttctctt gacacacata caatacaatt
     5821 cgaccgcaat aatcgacata ataatagctg taaagattcc tgaagttcct aaacgttcga
     5881 caaaagagcc catcctaata ccatttacta cagtttctgt ctcattgata gaagcaatca
     5941 agctgatttt accatcagtc atgatcaatt cagggataca cataaagaat gcaaaaacag
     6001 atagtaatgc ccccgtgagc ggattaacat ttaatttttg ttcatctcgt tcaattgacg
     6061 ttaattcata agcaaacaca atggcaaagt aaatagataa aatccccatc gtcattgtat
     6121 tgacgatcat gtacaagtca ctaactttga aaaaagttgc attaaaaaat ccttctaaaa
     6181 atgggaacgg taatggcaaa atatttaaaa ccaaaaacat cgagcctaca atcgtgaaag
     6241 gaatagaagc catccccgct gccatgacag cccgaacgat tttaagttgc gcaattttcc
     6301 ccataggtcc catcaggtat ttctgcaaaa aatcaaacat tagttattct cctttcctaa
     6361 atataacgct ttttcatatt gtttaatacg gatatactgt tggtcttggt tgagtgaaat
     6421 tttctttaaa cgatactgta taaatgcact ggcaaaaata ccgcttacaa caagaatgaa
     6481 aacaaataac tgtcctccat tgttatttgc cataaaagga tacaagaatg taaatcctga
     6541 ggtaaacaac actggaatca ctaataaatt aaagacactc tggaacaaaa aataccactt
     6601 tgtccatgga acgttcgttt ggtgttttcg ataaagcttt acttgttctc ctactgcagg
     6661 caaaatagct aaaatcagaa tcatcggtaa actacttcct aaacttttag ctgatagaag
     6721 cagaatcatc cattgcatgt tcatgaaaaa gaaaaaggca gtcaggtaac gtacgattag
     6781 ataacgattg aaatacatgt ttttcaaact aagttgcttt ttttctaatt cgattttcct
     6841 ttttactgta tcctgcacaa tgatccttcc tttctattta tttttttgat acaaataatt
     6901 catttcctgt gcaacttcca ataatgtcat cgtcgtcatc agatgatctt gtgcatggac
     6961 catgataatt tccatttcta tttttgtccc gctggcatat tctttcagaa gttgagtttg
     7021 tgactgatgc gcatgaagaa tctcttcatt tgcttgtcgc aacttctctt ctacttcttc
     7081 aaacatctct ttacgcatcg agcgaaatgc ttcatgtatc agtgtccgtg catttccgct
     7141 atggaaaatg atatcaaaag aagtcacttg tagtgtttca ctgtttattt tttcagccat
     7201 ctgttttcct ccttattcga tcagtttgat aaatttactg ctgaaattct caaaaatagg
     7261 ttcagctaag atttcctgct gtacttttag ctggtcaact aacctaacga ttgctttagt
     7321 cacgaccgta attccttcat tttcgacata agaaggcgaa actaaaaaaa caaaatgaat
     7381 ttcttgatat tgatcccact gcatcccaga aggaatcaat gcaacggcta cctttgtttc
     7441 gattccaact ggaacagcgg gatgtgggac tgccactgta tcactgaata tgatttgtcc
     7501 catattttca cgttgctgga tctgctttcg catttctctt atatatcctt cttcttcatt
     7561 tatgctcagc ttttgcagca aatcatgaag aatttcttcc tttttcggtt cgtacgtata
     7621 agtcttgaaa aatttttcag acaactgttc ggtgtaaatc tgcttttttt gctgtagaga
     7681 aaatgctggt atcggtctat ctgaggaatg atagggtata gattctccaa ctacctttcg
     7741 tattttacga acatcatctt cattcaaaaa aatactgaca tgtaaaactg gaattttgaa
     7801 aaacattgtc gaaagatcaa tagaagaaat aatcaggtcg attcctttta aagttgtttc
     7861 attgatctca taatagcctt ttacacttac cactgtaata tttttaccga attcactgac
     7921 tacgcgattc tttaatagtt gggcactgcc gtaacccgtt gcacagataa tcaatgcatg
     7981 tagtttatgc gcagctcttt ctttttccaa tgccgccatg agatgcaaag ccaaataggc
     8041 ccattcatct tcattgatgg tatagttttt caaagccggc atgttgctga aatgcctttt
     8101 tactgcgtca aagactttgg gattttcttt ctttatctcc tcagtcaatg ggttctcaat
     8161 gcgaatacct cggctcaatc gaatcatcat cggtgacaaa tgatcgagta gtccattgaa
     8221 aagctggaga tcctctacta atgaaaaatg aaattcctga gacaatcttt tcagaacttt
     8281 cgctaattcc tttgatagtt cctgatcatt ttgattttct ttgtgattcg acttagccat
     8341 gagatgtaat gtcaaataag caatttcttc ttcgggaaac gatacacctg cgattacctc
     8401 aatacgttga acgatccgct gcgccacttg atactctact cgcttagaaa cttcttgttc
     8461 gatgcctaat tccttgattt caaacccttc tcggagccgt ttgatcccca aagccaagtg
     8521 taaagtcaga ttctgtatga taaaatccga aacttttaac ttagcttctc ttgtttcatc
     8581 gagaatgata atgactagct cttcaaaact gatgtctggg aaaaaacggc tttgccctaa
     8641 atattctttc aatgaatttc cataatggtt tccaaaaaaa gtatccatga taaaatgccg
     8701 tcgatttctt tcttcccctt cgatccatgt cccttgacca tgcttagaaa cgatttttag
     8761 tgaatatgga atcaatcgct ccttgatttc ttgcatgtcc tttgtcaaac tggaacgact
     8821 gatataaagc tcttctgcca aatcatccat aaaaagaatg gcatcttcca aaagcaattg
     8881 gttcaatata tacttttgtc gatcttcgga ctcataaaat ccttgcggtt ctctagcata
     8941 tccaggtact atttcttgcc gactcaaaaa caagtcaaat gtcagtcttt gtaaaatatg
     9001 cagttggtag ccataacctg gtttagcaat gatcttacct ccgttttttt ctactaactc
     9061 tttgatccgc tgcaagtaat tacggattgt ccgatcagat aataacaatt cagaagctaa
     9121 acgttgactg gtgacatact ccttcttgtg atcaatcaaa tatctcaaca gcaatttttc
     9181 cttctgtttc attatcatcc actcctattc catcatatgt tctcttcgat cacctctgct
     9241 aacttctgga tgcctgtagg gattgggaca tacgcttgga aagggatact aacaacaggc
     9301 tttccaacag attctcctaa ttttttgaat ttatccaaaa acatcgtcgt ttgcgggctg
     9361 atcagaaata aatcaaaatc actattctta atcatattat cgccttctgt agctgaaact
     9421 gcatctacga gtacctcttc tcttttttgc ttaaagaaat ccgttgtctt tgaggccatc
     9481 atcgaggatg acattcctgc cgcacaaata atcaatgctt ttttcataaa tcaatcactc
     9541 cttttatttt tataaaccaa gcatatcttg taagcgtttt ctattcaaaa tatatatttc
     9601 cgtagcattg cggaaaaaat gtgtatcata tcttgaataa taatttctgg caccgtgata
     9661 tactagtgat agaatttttt caggagggga tttagtgagt atcacagtga atattttgta
     9721 tacaagtaca aatggacaag ccagaaaatt tgcagaagaa atggtcgaaa gaaacattgt
     9781 caaagcgatt cgtgcagaag aaggaaacga gaaatatgac tattactttc cactagaaga
     9841 tccagaatct cttttactga tcgaccgttg gaaagatgaa gaagcaatcg aaaagcatca
     9901 caaatccgaa atgatggctc aaataaacga actccgtaaa aagtataaat taaaaatgaa
     9961 agtcgaacgt ttcactgatt taccgtaaaa agatacacat ggaatgatag caatttatca
    10021 tccatgtgta tcttcacttt ttccttattt tttattcgat ctcatctaat gattcaaacc
    10081 gtgtttttct cttatttttc ggataagtat ttcagtcacc ccaatataca tgattcctaa
    10141 acatacccca aggatattct caaaaatgcg gatcgttact gctcctggaa ccccgaataa
    10201 actggcagtg attgccaaag caccaaaaga attaaaaact gttttatatc tataggtaga
    10261 acaaacacct aaagctagac cacctaatat accgacccag ttaaaaggaa taaattggct
    10321 gataccaata aataaaacac atccaatcaa tgagccaatg attcggtcta ctgctcgttc
    10381 tttgaaacca atcgacatca atccatagct agatacaata gaagaaaatg caaaccctgc
    10441 ccacatcaat cgctgaaaag ggacatactc tcctgcaacg atcaaaagac taatgccaaa
    10501 tgcatagatg agcatccaaa ttttttcctg cgaatacatg cctttcaaaa aaatctcttt
    10561 aaacaagctt ttgtctttgt tcttttctcg atgttttctg taaaggagaa ctgaaaacca
    10621 acaaaaaaac aaaaataata agctacttgt ttgtgtaaag aaattcttgt tcaacgaatc
    10681 tttaggtaac gaataaacaa taaagagata tgaaaagccg tacaaagaag cattccccat
    10741 tttgggatca ctagcggttg ccattaatat gctgcttagt aaaataaaat gaatcaacat
    10801 gcccataact gaccatttga tcagctggac atacggtgct actaatagtg agaacatgac
    10861 gattgctaaa cctatgatcg aatgggaaat cttataacca aagtcaacaa aacgaatact
    10921 catcaacata cagaataaaa ccacagcgta tggtttatgc ttcgattcaa ataatgttgt
    10981 aattataatg ataaaacaaa tggcaaacaa tacgatgaat aatgatcgta aaaacaatga
    11041 acaaatatac atgcactttt cttttttaga agctgcttct cgtatttttt gttttaaaat
    11101 aaacggatct agttgtagag actgataaaa atccacctgt tgtcgtcctt tctttttact
    11161 tttatttttt acagtcatct atgacaggtt gtaaatacta tggaacatca tatggcgttg
    11221 tcattttctt gtcaactatt tttcctattc atgtttttat atgaaaattt taggttatcg
    11281 ctgaaagccc cggaaatacg ggcatttcgc aggatatgag aactcaattt actaccaatt
    11341 tactactttc ggattgagcc gcatatatgc tgaaccatac tgctaatacc cgccacccca
    11401 aatacggcac atcataaatt gaatacgaca ccgtaaacgc ggcgttcctc tttcccgtca
    11461 tgggagaaca ggacgccgct tttttatgcc caaatagaga aaggaggcga cgccatgcta
    11521 tttacttccg gccctgaccg ggcgtttgaa cggctgatgc aggaaaagcc cggtttcgat
    11581 cactacgacg gcggcggggc ggacgcgccg gaggattgcg gcacctgccg attttaccgc
    11641 ccggattgga aatatcaatt ctgccagttt gcagagtgtc cctatcagcc gggcaagctg
    11701 accgactatg cagcggtcaa attcacagtg aaaggagcga acaacgctat ggcagttttc
    11761 cgtgtggaaa agaacaaagg ctatacggtg atgtcaaatc accacctgcg gaataagaac
    11821 ctgaccttaa aggccaaggg cctgctgtcg cagatgttgt ccttgccgga ggattgggac
    11881 tataccctaa aaggactgtc ctatatcaac cgggaaagca tcgacgcgat caggacagcg
    11941 gtatgggagc ttgaaaaagc tggatatatc cggcgtgagc agggccgtga cgcaaaaggc
    12001 aaaatggccg atatggtcta taccatctac gaacagccgg tattggaaaa cccggtattg
    12061 gaaaatccaa catcggataa cccggtgttg gaaaatccga catcggataa tccggcgtcg
    12121 gaaaatccaa cgcaattaaa taaagaagta caaagaactg acttatcaaa aaaagaaaaa
    12181 ataattacag atggacaaag tacccattcc attcctatcc catccccgac ccctgcccct
    12241 atggagggcg aggcagcggc tacgccgccg gaacggaaaa ggacaggaaa ggatgaggct
    12301 gtacggattt atcgggaaat cattttggag aacatcgagt atgcctatct cattcaggac
    12361 aggagcattg accgtgaaca gcttgacgaa atcgtagacc tgatgctgga aaccgtctgc
    12421 accgcccgaa agacaatccg tgtcgccggg gacgactacc ctgccgagct ggtgaaatcc
    12481 aagtacatga agctgaccgg cgaacatatc cgctttgtcc ttgactgtct gcgggagaac
    12541 acaacgaaag tccgcaatat caaacaatac ctgcgggcta tgctgttcaa cgcccccagc
    12601 accatttcca actactacac ggcccttgtc gcgcacgaca tggcacagcc cgattgggga
    12661 aagcctaaat ccggcctgcc ggactattcc tgcggcgagg gcgaaagcct gtaacgagaa
    12721 aaggaggacg accctatgaa gcagggcgca ttgatttttg acgagcgaac agaccgttac
    12781 gacatccgct ttgatctggc cgactattac ggcggcctgc attgcgggga gtgtttcgac
    12841 gtgatgatcg gcggcaaatg gaggcccacc cgcattgaaa tggccgaaaa ctggtatctt
    12901 gtcggcatcc gggccgacga cctttccggc ctgcgggtgc ggatttgacc tatatctacc
    12961 tgcggcgtct ggtggttccg ccttagacgc cgcttttgac tttctgacaa ggagggatgc
    13021 gattgcagga agaagttact caaaagacca ttgccctgtc catgaaaacg ggcaagctca
    13081 cggctcaagt gctgcaagcg gcgctgaaaa agtacctgca acaccgggcc aagggcaaaa
    13141 acacgctgca ccacggccag cagagcctaa agcagcttaa aaagcatggg gctgatctct
    13201 ccaacattga gatcaccgag gccaacattg gagcgttcaa gccatgcgcc aagaaatacg
    13261 gcgtggattt taccttacga aaagataaga ccacccagcc gccccactac gttgtgattt
    13321 tcaaggccaa ggatgcagat aaccttgatc tggcgtttcg ggagtttacc gccaagacgc
    13381 tttccaagga gcagcggccc tctatccgca aggttttgtc cgctgtgaag caaaaggcgg
    13441 cggcccagac caagcagcgg gccaaggaga aaatcaagga aagggggctg gaactgtgaa
    13501 gcctgacgta aagaaattgc tgatcctgaa cctcccgtat ctgatttttg tgtacctgtt
    13561 tgcgaaatgc ggggaggcgt accggctggc cgctggtgct gacctgtcgg ggaagctcct
    13621 gcattttgcg gatggttttg cggcggcttt tgccaacccg ctgccaagcc tgcacctgtt
    13681 tgacctgtgt atcggtatcg ccggtgccgt gatcgtgcgg ctggtggtgt actgcaagag
    13741 caagaacgtg aagaaatacc gcaaaggcat ggagtatggc agcgcccgct gggggacggc
    13801 caaagacata gcgccttaca tcgaccccaa gtttgaaaat aatatcctgc tcacccaaac
    13861 ggaacggctt accatgaccg gcaggcccaa agaccccaag acagcgcgga ataaaaacgt
    13921 gctggtgatc ggtggcagcg gttcagggaa aacgagattt ttcgtgaaac ccaacctgat
    13981 gcaatgcttt ccgaccacgg actatccgac ttcgtttgtg gtgacagatc cgaaaggcac
    14041 tttggttctg gaaaccggca ggatgttcca gcaggcgggc taccgcatta aaatcctgaa
    14101 taccattaac ttttccaagt ccatgaagta caaccccttt gtctacatcc attcggaaaa
    14161 ggacgtgcta aagctggtaa ataccctgat ttccaacacc aagggcgagg gggaaaagtc
    14221 agcggaggat ttttgggtga agtcggaacg gttgttttat tcggccctga tcggctatat
    14281 ctggtacgag gccccagcgg aggaaatgaa cttctcaacg ctgctggaaa tgataaacgc
    14341 cagtgaagcc cgcgaggacg acccggagtt tcaaagcccc gtcgatctca tgtttgagcg
    14401 tttggagcag aaagaccccg accattttgc ggtgcgccag tacaagaaat tcctgctgtc
    14461 cgcaggcaag acccgttctt ccatcctgat ttcatgcggt gccagacttg cgccctttga
    14521 catccgggag gtacgcgagc tgatggagga cgacgaaatg gagctggaca ccatcgggga
    14581 cgaaaagacg gtgctgtatc tcattatgag cgatacggac acgaccttta atttcattct
    14641 ggctatgctc caaagccagc taatcaacct gctttgtgac cgggctgatg atgtgtacgg
    14701 cgggcggctg cctgtccatg tacgcctgat tttggatgaa tttgccaaca ttggccaaat
    14761 acccaatttc gataaactca ttgccaccat tcgaagccgt gaaatatcgg cctctattat
    14821 tttgcagagc cagtcgcagc taaaagcgat ttacaaggac gccgccgaga tcatttcgga
    14881 taactgcgat agtgtgcttt tccttagtgg gcgcggcaaa aatgccaagg agatttccga
    14941 tgcgctgggg aaagaaacga ttgacagttt caacaccagc gagaaccggg gaagccaaac
    15001 gtcccacgga ttgaactatc aaaaattagg aaaggagttg atgtcccaag acgaaattgc
    15061 cgtgatggac ggcggcaagt gtatcttgca gctacggggt gtgcgcccgt ttttatcgga
    15121 gaaattcgat attaccaagc atccccggta caaatacctt gccgacgccg acaagaagaa
    15181 tacctttgac gtggacagat ttttgactat cacgcgccgg aaacggcagc aagtggtaac
    15241 gcaggatgaa gtattcgacc tgtatgaaat cgacctgtcg gacgaggacg ccgccgctga
    15301 ataaacagcg gcttttttca ttccaacaac aaaatcaaaa aatcattatg agccggaaac
    15361 cggcagaaag aggtaaattt atggcatttt ttaattccgc agttactact ttgcagacta
    15421 tcgtgatcgg actgggcggc gctctgtgcg tatggggcgg catcaatctg ttggagggat
    15481 acgggcagga caatccgggg gcaaaatcgc agggtgtcaa gcagcttgtc gctggcggcg
    15541 gcgtggccct gatcggtgtc actctggttc ccctgctgtc cgggctgctg ggctgatcct
    15601 ggcctttgtc cctgtccaaa cccttaacta atcgcctccg cgtcctcccg gccagaccgg
    15661 gagggcgcgg gaaaggaggt gctacccgtg attagtgaaa tcattgaaga atggataaaa
    15721 ggcatcctca tggacggtat cacgggcaac ctgtccgggc tgttcgatac ggtaaatgcc
    15781 aaagtcggag aaatcgccgc cgacgtggga cgaaccccgc aggcatggaa cagcggtatt
    15841 ttcaatatgc tgcacagcct gtcggaaacc gtgatcgtcc ccatagccgg ggccatactc
    15901 gcccttgtca tgtgctatga gcttatccag atgataacgg aaaagaacaa tatgcacgac
    15961 tttgacagct ccatgttctt ccgctggata ttcaaatccg cgtttgcaat cctgatcgta
    16021 acgaacacat ggaacatcgt catgggcgta ttcgacgcca cccaaagcgt cgtcaatcaa
    16081 agcgccggtg tgattatcgg gcagacatcc attgatttta acaccctgat accagacctt
    16141 gaaagccaat tggaggctat gagcgtgggc gctcttttgg ggctgtggtt ccaaaccctt
    16201 gttgtgggcc tcaccatgaa tatcctttcc atctgcattt tccttgtgac ctatggccgc
    16261 atggtggaaa tctatgttgt cacgtcgctg gggcctatcc cccttgccac aatgggcaac
    16321 gcggaatggc gcggcatggg ccaagggtat ctgaaatccc tgctggcgct gggcttccaa
    16381 gcctttttaa tcatggttgt ggtgggtatc tacgcggtgc tgatacagaa tatcggttcc
    16441 gcttccgaca tatccggcgc aatctggggc tgtatgggct atacggtgct gctgtgcttc
    16501 tgcctgttca agaccggcag tatatcaaag gccgtattcg gggcgcattg atcccaaaat
    16561 ccaagcatac acaggaggaa cttgtttatt tcaacgtaca acatcattta cgctgacccg
    16621 ccgtggcggt atgactgcaa aaacgtacag ggcgcagcgg aaaatcacta ttccaccatg
    16681 agcattgacg agctgtgcgc cctgccggtg gaacggctgg cgtccaaaga ctgcctgctg
    16741 tttttgtggg ccacattccc catgctgccg gaagctctgc ggttaatcaa agcgtggggc
    16801 ttttcattca agaccgtggc ttttgtatgg ctgaaacaga accgtaaaag ccttacatgg
    16861 ttttacgggc tgggccgctg gacaagggga aacgcggaaa tctgcctgct ggcaacacac
    16921 ggcaagccga agcgtcgttc tgcctctgtc catcagttta ttatcagccc gattgaacaa
    16981 cacagcaaaa agccggatgt gacccgtgaa aaaatcgtag agctggcggg cgatttgccc
    17041 cgcgtggagc tgtttgccag acagaaaacc cccggctggg acgtgtgggg gaacgaggtg
    17101 gaatgtgacc ttatcctgac gtgaaaggag gtgccaaatg gcttatgtaa ccgtccccaa
    17161 ggacttaacc catgtgaaaa gcaaggttgt gtttgggctt accaaacggc aactgatctg
    17221 tttttccggg gcgctggtga tcggcgtccc cttatatttt ctcacccgtg actatcttac
    17281 caacagcgcg gcggccctgc tcatggtatt tgccatgctg ccgggctttt tgcttgccat
    17341 gtacgagcgt cacggccagc cccttgaagt ggtgattatg cagatcatcc agtgctgctt
    17401 catccagcca aaggagcgtc cctatcagac cgacaacacc tacaccgccc ttgtgcggca
    17461 ataccagatg gaacaggagg taaaggccat tgtccaaaaa ggaaatccca agagaaaccg
    17521 aaaaaacaaa gctgacccgc gcccagcgaa aagaaattga cgcggtaatc cgtaaatata
    17581 agggcgacgg caggccccac acggcgcagc agagcatccc ctacgaggtc atgtacccgg
    17641 acggcgtgtg ccgggtatcg cccggcgtct tttccaagtg cattgagttt gcggacatca
    17701 gctatcagct tgcccagccg gacacgcaga cggctatctt tgaaaaactg tgcgacctgt
    17761 ataactatgt ggacgcctcc attcatatcc agttttcttt cctcaatcgg aaagttgacc
    17821 cggtgcagta cgccaagagc tttgagatcg cgccgcaggg ggacgacttc gacgacatcc
    17881 gggccgagta caccggcatt ctgcaaaagc agcttgcaaa cggcaacaac ggcatggtaa
    17941 agaccaaata cctgacattc accatcgagg ccgaaagcgt gaaagcggcg cgagccagat
    18001 tgaagcgcat cggctttgac ctgttaggct atttcaagag catgggggcc gtagcccatg
    18061 tgatggacgg ctgggaacgg ctgaacctgc tgcatggcgt gtaccacccg gacggcgaga
    18121 tattcaattt tgactggaag tggctggccc cgtccggcct ctccaccaag gattttattg
    18181 ccccgtcctc cctgtgcttc ggcaatgcaa agacattcgg catgggcggc aaatacggcg
    18241 cggtaagttt cctgcaaatc ctgtccccgg agctgtcgga cgatatgctg gccgacttcc
    18301 tcaatacgga aagcggtgtt ttagtcaatc tccatgtgca ggccattgaa cagaccaagg
    18361 cgatcaagac catcaagcgc aaaattaccg accttgacgc tatgaagatt gcggagcaga
    18421 aaaaggccgt ccgcagcggt tacgatatgg acattcttcc cagcgacctt gccacctacg
    18481 gcgaggacgc caagaagctg ctgacaaagt tgcagacccg caatgaacgg ctgttccagt
    18541 tgacgttcct tgtcctcaac gtggccgaca ccaagcaaaa gctgaataac gacgtgttcc
    18601 aagccgcagg cgtggcccaa aagcataact gtccccttgt ccggctggac taccagcagg
    18661 aacagggcct tgcaagcagc ctgccgctgg gcgtcaatca gatcaagata cagcgcagcc
    18721 ttacgacttc cagcgtggcc gtgttcgtcc cctttgtgac gcaggagctt tttcagggcg
    18781 gcgcggccat gtattacggc atcaacgcca agtcccgcaa catgattatg ctggacagga
    18841 agcaggcccg ttgccccaat gccttgaagc tcggcacccc cggcagcggc aaatccatga
    18901 gctgtaaatc cgagatcgtc agtgtgttct taacgacgcc tgacgatatt tttatttcag
    18961 acccggaggc cgaatattac ccccttgtca aacggctcca cggacaggta atccggctgt
    19021 cgcctaccag caaggacttt gtgaacccgc tggacatcaa cctgaattat tcggaggacg
    19081 ataacccgct ggccctgaaa tccgactttg tgctgtcgtt ctgtgagctt gtcatgggcg
    19141 gcaagaacgg gctggaagcc attgaaaaga ccgtgataga ccgggctgtg cgcgtgatct
    19201 accggcccta tctggcagac ccccgcccgg agaatatgcc gatcctgtcc gacctccaca
    19261 aagcgttgtt agatcagcat gtcccggagg ctgaccgggt ggcgcaggcc cttgacctgt
    19321 atgtgtccgg ctccctgaac gtatttaacc accgtaccaa cgtggacatc ggcaaccggc
    19381 ttgtgtcctt tgacattaag gagctgggca agcagctcaa aaagctgggg atgctcattg
    19441 tccaagacca gatatggggg cgcgtcacgg ccaaccgaag ccaaggaaag gccacatggt
    19501 attttgccga cgagttccat ttgcttttga aagaggaaca gacggcggcc tattctgcgg
    19561 agatttggaa gcggtttagg aaatggggcg gcattccaac aggtgccacc cagaacgtca
    19621 aagacctgct ttcctccccg gaaattgaaa atatccttga aaacagcgac ttcattacgc
    19681 tgctcaatca ggcgtccggg gatagaaaaa tccttgcgga acgcttgaat ttgtccaccg
    19741 aacagcagaa gtacatcgac aattccgaac ccggcgaggg ccttttgatc tttgaaaacg
    19801 tggtgctgcc ctttaccaac cccatcccgc acaacaccca gctttacaag attatgacga
    19861 cccgcttaaa cgaggtggcg ggagtatgac ccggcgacgc cacaaaggag gtgaaccacc
    19921 atgaaagagc tgaaagccaa ggcgaaagtg acgcaggcca tgacccgcga cgggctgacg
    19981 ctggaaaatc aggccaccgg cgaggtggag aacatttcca gccgggaggc cgaacaggat
    20041 ttgtccgcgc cggggccggg cggcaccgcc gataagctgc tggaacgggc cgacgccatc
    20101 catgacggcc ataaggccaa gaaagccgcg aagcaggccg gggctgtggc cgccgagggg
    20161 gacgctgccc tgcaccggcc atccgcccgg ctccaactgt cggaggaaga acgggccgac
    20221 ccggatttac agccctatat ccgaaaatcg gacaaacggg ccgataagct ggataaggcc
    20281 cgcgccgcca tccccaagaa gcgcgtcccg gtcaaggaaa ccgtctttga cgaggcaagc
    20341 ggcaaagcaa agaccgtcct gcactttgac aaacaggaca aggggcctcc ctccctgaaa
    20401 cccaatccgg caggccgtcc tgtttccgag gtttttctat tcggccacgg gaaaatccat
    20461 gaagtcgagc atgaaaacgt gggtgtggag ggcggccata agggggagga acttgccgag
    20521 cggcaggccg gaaaagccat ccgcagcacc atccggcacc acaggctgaa accctactgg
    20581 gacgcggaaa aggccgagcg caggctattg tctgccaacg cggagtattt ctaccagaaa
    20641 tctttgcggg ataacccgga gatcgcacag gcggccagca accccatttc ccggttctgg
    20701 cagaaacagc agatcaagcg ccagtacgcc aaggccgccc ggacagcggg ccagaccgca
    20761 ggcgcggcgg cacagggcgc ggccaagaca gccggggcct ccacgaaagc ggccaaggcc
    20821 gcagccaaag aaagtgaacg ggcggcctcc tttgtggccc gccactggaa aggggcgctg
    20881 gccgtccttg tgttggggct tttgatgctg cttatcatcg gcggcttaca gtcctgcacc
    20941 gccatgttcg gcagcgccgg aagcggcatc acggcgtcct cttacttttc cgaggatagc
    21001 gatatgttgg ccgccgagga cgcctacgcg gagctggaag ccgagctgca agactatctg
    21061 gacaactacg aggccaccca tgactatgac gaataccatt ttgaccttga cgagatcagc
    21121 catgacccct atgtgctgat ttccattctg tcggcgctcc atgacgggat atttaccatc
    21181 ggggaggtac agggtgattt gtccatgctc tttgacaagc aatatatcct gacagaaaac
    21241 gtcgtggtag aaacccgtta ccgcaccgaa accgatacat ggacggacgc ggagggcaac
    21301 acccatacag acacctacca agtgccgtat gactattaca tctgctacgt cacgctggaa
    21361 aacttcgacc tgtcccatgt gccagtctat atcatggacg aggaaacgct aagtttctat
    21421 tccctgtata tgtccacgct aggaaaccgg cctgatctgt tttccggcag ccagtacccc
    21481 aacgcctcca ccctgacgcc gccgacctac tacgacgtac ccccggaggc tttggaggat
    21541 gccacctttg ccgccatgct caaagaggcg gaaaaatata tcggttatcc ctatgtgtgg
    21601 ggcggcagct ctcccagcac cagctttgat tgcagcggct tcatttcgtg ggtaatcaat
    21661 catagtggat ggaacgtggg acggctgggg gcgcagggcc tatataacat ctgcacccct
    21721 gtttccagtt cgcaggccaa gcccggcgac cttgtatttt tcaagggaac ctacgacacc
    21781 cccggcgtgt cccacgtcgg cctgtatgtg ggcaacaatg tgatgctgca ttgcgggaac
    21841 ccgatttcct acacgaacct tacttcaaac tactggcaat cccattttta cagttacggg
    21901 cgtttacctt aacgccagaa aggagctttc aattatggca accaaacttg accgcatcga
    21961 aaaggacatc cagaaaacca agtctaagat cgcggagttt cagaaacagc ttcgggagct
    22021 ggaaacgcag aaaaccgaac aggagaattt gcagatcatc cagcttgtgc gcggcatgaa
    22081 catgaagccg gaggaatttg ccgcgttctt acgcagcggc gcaatgcagg ccgcccctgc
    22141 cgcaacccct taccatgaac aggaggacaa tgcccatgaa gaataagacc agacggattt
    22201 cccgtattct ccccgcactt ctggccgtga tgctctgcgt cacggccttt cccgttaccg
    22261 cccttgcagg cggcaatgac ccggcaccgg ccccgctgcc ggaggctaca accgaacccg
    22321 ctacgggcgg tttggagcct gaaacagacg aaaccggcac cgccacggac gggacagaaa
    22381 ccgaggccga aaaagatacc ggccctctga ccggcaagga catcaccgac ctgttttccg
    22441 cgctgttcgg ttccaaggtg agcattgccg ccacggacga cggcatccag atcaccaccg
    22501 gcaccgacaa aaaagaacct gaacagaccg gcaccgtcac caccaacggc ggcaggctga
    22561 atgtccgcac cggcgcgggg cttgacaaga ccgcgttcac ccagcttccc aatgggacga
    22621 cggttgaggt agtcggcaca gacggcgact ggattaaaat tctcttgccg gagcgcatcg
    22681 gctatgtcca ttccgactat atgaccgtca gcgaaaagga agtagcggct accggcgagg
    22741 gcggtttttc cctctccatt gacccggagg aaattgcttc tctgctggaa ctgttcaatg
    22801 gcggcggggc cagcgccgcc ctgtccccgg acgggaacct gtcactgatt gacgacatcg
    22861 gcagcgccgc gaaaaatggc aagcagttta tcaccgtgga aaccaaaaac ggcaatgtgt
    22921 tttatctgat tattgaccgg gacgacgagg gaaaagaaac tgtccatttc ttaaatcagg
    22981 tggatgaagc cgatctgctg acgctgatgg aggacgggga aaccgtcacc cccgctgctg
    23041 tctgctcctg taccacgaaa tgcaaggccg gggctgtgaa cacgaattgc ccggtatgta
    23101 aaaccaacct gaccgagtgc agcggcccgg agccgcagga accccagccg gaggaacccg
    23161 aagccccgga ggaagaaccc aagggcggcg cgggcggcct gatcgtgttc ctgctggtgg
    23221 cgctggcggg cggcggtgcg gccctttact atttcaagtt caaaaagccc aaggctgaaa
    23281 ccaccggcag cgacgatttg ggcgagtatg attttgggga ggacgaggat ttggaggacg
    23341 aggaacccga aaccgagttt gacccggaag ctgattttgt gagtgaacag gaggacgagg
    23401 aatgacatat aaacttgtaa tcgcggaaaa acccagcgtg gcccgcagca ttgcgggcgt
    23461 gatcggggcc acggagaaac acgacggcta tttggagggg aacggctatc tggtgagctg
    23521 gtgcatcgga catctggtgt cctttgccga cgccggacgc tatgacgagc gtttcaaaaa
    23581 atggcgctat gaggatttgc ccattctccc ggaaacatgg cagtacatcg tccccgacga
    23641 caagaaacag cagtttgacg ttctctgttc gctgatgcag cgccccgacg tgaccggcct
    23701 tgtgtgcgcc accgacgccg ggcgcgaggg ggaattgatt ttccgtttcg tctaccagat
    23761 ggcgggatgc caaaagccct ttgagcgcct ttggatttcc tctatggagg acagtgctat
    23821 taaggacggc tttgcccacc tgaaaccggg cggcgactat gatccgctgt atcaatcggc
    23881 cctgtgccgg gccaaggccg actggctgat cggcattaac gccacccggc ttttttccgt
    23941 cctgtaccac aagaacctga ctgtgggccg cgtccagacg ccgaccttaa aaatgctggt
    24001 ggacagggac gccaagatta ccgatttcaa gaaagagaaa tatcacatcg tccatatcac
    24061 ggcgggcggc gcggacgcgg taagcagccg cttttccgac gctgccgagg caaacaccgt
    24121 caaggcggct tgtgcaaaag cgcaggccgt ttgcgtttcc gtgacgcggg agaaaaagac
    24181 ggaacagccg cccaagctct atgaccttac caccttgcag cgggaggcta accggctttt
    24241 cggctatacc gccaagcaga cccttgacta tgcccaggca ctgtatctaa agaagctgct
    24301 gacctatccg agaacggata gtcaatatct taccgacgat atgctgcccg ccgtggaaag
    24361 ccttgtgtcc ggcctgtggg agctggtgcc gtttgccaag ggcctgaacc tttccccgca
    24421 gtttgaccgc atcctgaaca gcaaaaaggt gtccgaccac cacgccatta tccccaccgc
    24481 tgaatttgtg aagcagggct ttggcactct ggccgagagt gaaaaaaagc tgctgtccct
    24541 gatctgctgc aagctcttat gtgccgtggc cgggccgcat atctatgagg ccgtcaccgc
    24601 cacatttacc tgtgccgggc aggagtttac cgccaagggc aagaccgtcc tttcccccgg
    24661 ctggaaagag attgaacgcc ggttccgttc ttccttaaag accgacgccg acgaggacgc
    24721 cgaggcggtg cgggagctgc cggagctttg cgagggccag acctttgcag acgtggccgc
    24781 ctctgtcacc gagcatttca ccacgccgcc caagccctac accgaagata cgcttttgtc
    24841 cgctatggaa cgggccgggg ccgaggatat gccggaggat gccgagcgca agggattagg
    24901 cacaccggcc acccgcgccg ccattctgga aaagctggtg cagatgggct ttgtccagag
    24961 aaaaggcaag cagctcattc ccaccaagga cggcatcaat ctggctgtgg tgctgccgga
    25021 ggcgcttact tctccccagc ttactgccga atgggaaaac cggctgaccg agatcgccaa
    25081 ggggcaggcc gatcctgacg aatttatgga cggcatcgag acgcaggcgc gggagctggt
    25141 gcagacctat tcctgtatca gtgaggaaaa gcagaaattg tttcaggccg agcgtgttcc
    25201 tatcgggacg tgtccccgct gtggtgaggc tgtctatgag ggcaagaaaa actactactg
    25261 cggaaaccgg gcctgccagt tcgtcatgtg gaagaatgac cgattttttg aggaacgcaa
    25321 gaaagcgttc accccgaaga tcgccgctgc gctgctcaaa accggcaaag tcaaggtaaa
    25381 gggcctctat tccgtaaaga ccggcaaaac ctacgacggg acggtgcttt tggctgatac
    25441 cgggggcaag tacgtcaact accgcattga acacaggagc taaaggacag taacacagca
    25501 agcataggga gtgtggctac actcccggta aaatcccccg gccgcgtccc tgccgggccg
    25561 gggatttttg cttgttgggg aagtccccaa ccccctattt tttgaaacag aacagagaaa
    25621 ggagtgaccg cgtgaacgaa gtattttcta tccaagtcca taaccggcag tttttggaac
    25681 agggcaaaca aggcgtctgg ctggatttgc ccaccacaac cgaaacgctg caagccgccc
    25741 tgcgggatgt tggcattact gccgacaatc cgcaggattt ttttatcaac gggtattcct
    25801 gcccggagga cagacacctt gccattccct atgacatggt gctggccgcc gacgtggacg
    25861 agctgaattt ccttgccgcc cggctgggcc agcttgacgc cgccgagatc gcagagctga
    25921 acgccgccct gcaaaatccc cgtggcggct ttgagaatat cggccagatc atcgactaca
    25981 ccgaaaatgt ggattactat gtgcatcttc ccgacgttag gggggatgga cagttgggcg
    26041 actactacct gaaccgttcc ggcatggtgg atatgccgga ggaatggaaa gccgggattg
    26101 atacggcgca gtttggacgc cacattgccc agcaggaaca aggcgttttt acccagtacg
    26161 gctatctggt gagaagcggc gacgaatggc agcgcgtcca tgagggccag ccggtgccgg
    26221 aggaataccg ggtgatgcac tacccccagc cggaagctga ccgggaggaa ccgcaggccc
    26281 agcagccgga ggcatccgaa agccccgaca aagcgccgcc cccggttatc ccgatcatgc
    26341 tggacggcat gaacagcaaa gaacgcatga aagtgattac tgaccgtctg gaaaccggcg
    26401 tacaggagct ttttgaaagt agccgctata tggcttactt atccgctatg tccaagatgc
    26461 acgattacag tctgaacaat acgctgctga tcgccatgca gggcggttcg attgtggcgg
    26521 gctataacag gtggcaggat gaatttgacc gccatgtgaa gcggggcgag aaaggcatta
    26581 agatactggc cccggccccg ttcaaggtta agcagcagat ggagaaaatc gacccggcca
    26641 cccagcagcc cgtgatcggc gcggacggca agcccgtgac cgaagaacag gaagtgacgg
    26701 ttccggcctt taaggtggtg acagtctttg acatcagcca gaccgagggc aaggattttc
    26761 ccatgcccta tatcagtgaa cttaccggcg acgtgcagca gtatcaggat ttctttgccg
    26821 cgctggaacg gacatcaccc tatcccataa gcattgagcc gatttcagac ggggccaagg
    26881 gccgctgctt ctacgcaaaa cagcgcattg ccatcaacga gggcatgagc gaattgcaga
    26941 ccctaaagac cgccatccat gagattgccc atgccaagct ccacgccatt gaccgggagg
    27001 cccccgccga ggaacaggcg gcccggccag accgcagcac ccgcgaggta caggccgaaa
    27061 gcgtggccta taccgtctgc cagtattacg ggctggacac gtcggactat tcttttggct
    27121 atgtggccga ttggagcagc ggcagggagt tatccgagct gaaagcctcc cttgaaacca
    27181 tccgggccac ggccagcgaa ttgataaccg agatcgacgg ccattttgcc cagcttcagc
    27241 aggagcgcga ggcccggcag gaacaggagg ccgtcacggt tgacgttacc ccggaggcag
    27301 cggccccggc gcaggccgag gaagaacagg ccccggccct tgatccggca gccgagccgc
    27361 ttgtgactgt gctatggagc gaaagccccc agctaaagga cggccagcaa atgccgttgc
    27421 atgaggccga cgcgctgttt gcccgtctgg atgcagcata tccggcagag gcaggctatg
    27481 acaaaaccgc gttccgcatt gactgtatct ttcagggcga gccggacagc tacgaggggc
    27541 ggcaggattt tggagatagg gacggttccc tgatcgaaca tatccagagc taccaccaat
    27601 tttacgcgca ggacgaaaac tggaaaaagc atgtaatcgc tacaaccggc atagagggct
    27661 gggagcagga caaggcccat cgggatatgc tgttaggcga gtttgtcccc tatatgaagc
    27721 tgcattgcag ccttgcccaa atggaacaga cggcgcaggg ccgcttacag tccggggaaa
    27781 ccctgacgcc ggaggaaacg gcctatttgg gggcgcttct ctcttatgtg caggagtgcc
    27841 gccccatgct caatcagggc cagtatcagt tgccggagcc gccgaaactg gctgacttcg
    27901 accagagctt gcaggactac aaagcacagg tacaggccga gatcgcgcag gaggccgccg
    27961 cagccggtat gaccgtggag gaatacgcgg cggctggcta tgaagcaccc caacaggaca
    28021 ccttttccat ctaccagctt cgaggcgggg aggaaacccg cgactatcgg tttgagccat
    28081 acgagcgttt acaggccgca gggctggccg tggacagtgc caactatgac cttgtttata
    28141 cggcaccgct ggataaaagc acgtctttgg aggatattta ccgcacattc aacatcgacc
    28201 acccggcaga ttttagggga cacagccttt ccgtttccga cgtggtggtg ctgcaccaga
    28261 atggacagga caccgcccac tatgtagaca gcttcggcta tatggaagtg ccggagtttt
    28321 tgcgggaaaa tcccctgcgt accgccgaac tgtctacgga gcagaacgaa aacatgattg
    28381 acggcattat caacaatacg ccgcccgaac cgcagccgga ggaacccgaa cagctatctt
    28441 ttgaggatgt ggcagttgct tccggccaga cacaggagga acccccggag gctgcgcccc
    28501 cgcaaacggc tgtccgctat tatcccatca atgagggagc cgcccgccgc gccaaggaag
    28561 ccatgagctt ttctgactac cggcccggca gcgccacggc ggaataccgg cactatgtcg
    28621 atcaggccgt ggaaattgcg gagcgccaga aaaagcgtgt ggagccagag tatcacgaaa
    28681 agatagacag cctccttgac acctacgccc gcaagctggc cgagaacatg aataagggct
    28741 atgagattac cgcccgcgtc ccctctgtcc tgattgccgg gccgtccaat ttcccggtac
    28801 gggcgaaaga aaagcagaac gccgccagtg accgcaacat ggaggaattt cagtacattc
    28861 agggcttgct tgacaaaatc cgcagcaccg gcatgggtgg tatcagcgcc gacgatccgc
    28921 aggccgtttc caagctggaa aagaaactgg aaaagctgga agcgtcgcag gagttgatga
    28981 aggccgtcaa tgcctactac cgcaagcatg ggacgctgga cggatgcccc catctgaccg
    29041 aaagaggcat tgaaaacctg aaagcggata tggcgtcggg ctggcactat gagaaaaaac
    29101 cgttccagtc ctggcagctc tccaacaata acgccgagat acgccggtta aagggccgca
    29161 ttgaggaatt gacccgccaa aaggaggcgg cctatgtggg atgggagttt gacggcggca
    29221 ctgtggagat caaccgggtg gccaaccgcc tgcaaatctt ctttgagggg aagccggatg
    29281 ctgctgtacg cgacgagctg aaaagcaacg gtttccgctg gtcgcccaag gccggggcat
    29341 ggcagcggca gttaaacgac accacaatcc gcgtcgctga ccgtatcaag tgcatccagc
    29401 ccctttccgg cgaaaagccc agcgccttac agcttgcggc ccgcagggag aaagaaaagc
    29461 cctctatccg ggcgcagctc aacgcggcca agaccgaaca ggccaagcag ccaccggcgc
    29521 gggaaaaatc aaagggattg gaggtagagt aaatgcagaa gtttgaaaat gtggacgttc
    29581 tggccgcgct ggaacagatc atgcggcaga acaccgcgtt ttatcaaagt gactttggca
    29641 ttgacaaaga cattatcagg cgggatgccg ccagcgatca ggccgcagac aagaccctgc
    29701 tctggatgtc ccggccctcc gggacgtact gtttccggga acgtgatgtt ttcctgaaag
    29761 acacccgaca gtacaacaca tggaaatttt acggggagca gacccgtgac aagatactgg
    29821 cctacgcggt ggagctgacc ggcacacagg gcggcactat ccggggcaat ctctatgagc
    29881 tggactatcc gcagcatttc cggcacgtca ttgaaaccgc ccagccggtg agtgtgaacc
    29941 ggctctttta tgagcatggc acccgtgaca tcccggagag ccagcgtttt gacggaagcc
    30001 ctgaccgggt attgggaaac tttttgtact atgaggccca gccccatgac ccggctgtgc
    30061 tgcaggaggc gctacggacg gagcagcaca gccgggggcg ggccgtcccc ggcgatttca
    30121 aagtccacgt caccgctttg catgacagcc ggattgaaac cgaggcccgg cgtattgtgg
    30181 acagcctgaa agatctgtcc gcgcctaaca gccccaataa aacgcatttc atggtggaga
    30241 tctccccgca ctttgcggcg attgcttcca ccaaggataa tgaccggctc tttgccatgc
    30301 tgccctataa atccctgtgt tttaccggga tgaaaggcag gcatggcgtg tatgccgtga
    30361 ttaaccggga cgagcgccgg gatgtgagta ttcgcaggcc ccggccctct atccgcaagc
    30421 agctatccga cagcaaacag gccgccagcc caaaaaaggc ggcggccaga accaaaaaga
    30481 atgaattgga ggtataagga atgatgcact ttaccgtgga ggaagaaaac ctgatctgta
    30541 tgtaccacaa tgcagaccgg cgcagaacga taaacaggct caccgccgtc atgccggtta
    30601 tggatgggga tatgcgggcg caggcccagc agaccctttc caagctggaa cgcatgacgg
    30661 atgccgacta tgacggccag aagttccact tcacagacga ataacgaaca gcagacgccg
    30721 ggcgcggcgg ccatgtggtc gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc
    30781 atttagaacc cggtattgtc ggggatagga tgaagtattc gcttgcccct gcaaacatgc
    30841 gggagaagcc ttgcagcggg cggttttcaa tcccttaaat gcgtaggttg cagctataat
    30901 tagacccctg aaacagatat agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt
    30961 gatagccgca aagcggcgta agagggtgca gccctcttgc cgtctatctc tgttccagcg
    31021 ggaagctcgg caatgcttcc agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa
    31081 tgactttctt ctttttgccg tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt
    31141 agtcagcata atgggccaac actttttccg tggcccacct ctcaccggca acggcggctt
    31201 tgatcgtttc atagtccagc agcttttctt gtccgtccat cctgataccc ccgtcagact
    31261 gattttttct atgataccat attcggaagc atggaaccag agaaaacggc gcttttctgc
    31321 tggtaaattc tacgcattta gaacagcact tcggcgggga taggataaaa cacccacata
    31381 ccccgctgac catgccggaa aagccttgaa atataagggc ttttacagct ctaagtgcgt
    31441 aggtttccag tatcttttcc gctggcgctc ggcctctttg cggcggcgca tccgtttgga
    31501 gcaggccgcg cagtatttgg cccggttaga cttcggcgtg aacgtggccc cgcactcaca
    31561 gcagcgtttc acttcgtccc ggtctttggt gatctcggca tacagggccg catccagcgg
    31621 gaggacagcg gccttaaacc agcggcacag aagcgaatag gaaatgctct gtacgcaaat
    31681 acattcttcg ccgtcgtcca gcaacaggca gttgccgtca caatagttgc agcactcatg
    31741 cgtcagcttc cgggccttgc gatattgagg gtaattcatt cgtggaatac tcatagctct
    31801 tgcgtccatt cctttcccgg cgtcatacgc aaaatgccgt ccacattctg cttgatgatg
    31861 ccgtattcct gcatctgctt tttcgcttcg ccgtacttct catacagccg ttccttttcc
    31921 tcggccaatc gcttgtactc ggctttcagg gcgtacagat cggggagctt tttcacaccc
    31981 tgtcccttta gggcctttgc cgcagcttca tacaaaatca acgtgctttc atgctcacgc
    32041 cggaattttg ccttatccgc tgcattgcgg tattccatag ccacgggcca gagctgcttg
    32101 taggtagaaa tattctttat cagcaccgcc atatccgcaa gccggtgttc catagctttg
    32161 agggtagcgg cggcctcgtc gctggcggcg gtgatctcgg ccactttgct ttccaaatcc
    32221 gggtaggtgt ctatcccatg ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa
    32281 ttgtggagct tcgcccacct ctcatagcct gccgactgct gggccttaat gttgttttcc
    32341 agatcaatcc gcaaagaaat ccccttgcgc tctttgggcg ctctggtgat aacgcggcct
    32401 ttgatacgct ctttgatggc ttcctcggtg taggcttccc ccaatgtctt gcagcgggta
    32461 aagcgttcct gtccgggagc gcggaaagaa ataaactttc ccggcttgat ctcatagccc
    32521 tgcgcctgca tgagccgcag gaaatcatca aaatcttttg cctgcgggat agcggcgtca
    32581 atggctgctt tgagcttcgc tttccagctc gtccctttcc gctgggcgtc ccactcggca
    32641 tagctcttgc ccttactctg gccgggcttc accacggaca atccgttttc cttgcacagc
    32701 ctgtcgctgg tgcgccggat ataggcatag ctctgtttgt tggaaatgta cttgcgctga
    32761 tttaccatat ccacggcaca gaaaataatg tgattgtgga cgtggccctt atcaatatgg
    32821 gtagtcagca catagggata tttcccttgc agcacttcct cggcaagctg cttcccgatc
    32881 tcatgggcct gttccggcgt ggcttcgccg ggggcaaagg attgtatcag gtggtggccc
    32941 agattattgc ccttttggta tgcctgcgcc agcagcattt caaattcaat gtccgctgtt
    33001 tcataagagc aggcaaagga ggccaccagc agcttgccgt cagttttgtc cgggttctcg
    33061 atgtagtcaa gggctttttt cagcgttgac ttgataggat gtatctttgt aactgccata
    33121 tctgcgccag cgcccccttt atatcctcga tgtcctgtgc gtacaccggc cccataccgt
    33181 tcagcctctt tgcaatctga ttgacgttga cgccgatctt ctgtatctcg gcggtctgcg
    33241 cccggatgtc gctggtgtcc atgttgacga tatagccgtc aatgagcatc ttccgggcgt
    33301 aggctgaaaa gttcttcgta tgcagcagcg ccattttctg ttcaatcata gcccgttcct
    33361 ccggcgtcac agggacgcgc agcacgattt ttcgctttct gtttgccatg cttcaccgtc
    33421 ctttccattc aggggtttgg ggatttcccc aacaagcaaa acggacgcgg cccggcaggg
    33481 acggggcagg ccgtttttcc gggagtggct acactcccta tgcttgctac gttactgttt
    33541 ctccactaac aagacgtttc caaagcccca aactacccgc aaaatagccc tctggaaaga
    33601 aaaaagtgca aaaaaataga agctacgctg tgcagctccc gctaaaagta aaacagagga
    33661 tgccagttac acatcctccg tttcattctt cttcggcttt tcaattagta tggcgctgtc
    33721 aatcgcgccc tcgatgatcg gcaggtattc ttcggggcag agtttcagct tgtggctgac
    33781 cctttgccgc agttcgcttt cctcccgcat gatctccggg ttaaagtagc gttccacagg
    33841 aagcccgcag gctttaataa gctgtatgac aaccggcaga ctgggaatgg ccccgtcgtt
    33901 ttcgatgttg gcaagatagc gccagtcaat atttattttc tctgccattg ctttgcgcga
    33961 aacgcgcttt gcttgccggg ccgctttcac atccgcgccg aaagtttcaa agccgggaca
    34021 atcttcaata ttcgccatat cgcacctccc acttacattg tatatttcat actctgcccg
    34081 tggaatgttg gtatatgcac ctttcttaaa gtttatattt cacattttgt ttcttttatt
    34141 cttcgagcaa aagaattata atgtaccctg tggaggtgta ctatggacta catgacgttg
    34201 aaagaggcag gcgagaaatg gggtgtgccc ccacgatgga taaactacta ttgtgctgcg
    34261 ggacgcatcc ccggcgctgt aaagatggcg acgatctggc tcattcccaa aactgcggaa
    34321 aagccgattg acaggcgtac aaagaaagga aaggcggtta ctgaatagta taatgaaacc
    34381 ggcgcatttt ggatatggtt ttgaagagag agaaagcatg gtattttgtc acggaaaaac
    34441 tataagatga tagatggcag gctgctccag accgataaaa agtattctca tctgaaacag
    34501 aaagaaaaga tttacacatg gatgtatgaa gaagcgaaac tttatcatga cagaaatgga
    34561 aaatgcccga aacaaagacg aggaagctgc tgctgtatca gccgtatatg gtagaattga
    34621 aaaggccggc atctggattc tttatggtga agtctccaga cattggataa ctgctggtct
    34681 aatgcgcctt tgagtatggg gtgattgatt ttaataccgg ggaggtacgg ctttttgagg
    34741 aaaatattct gggatttaca tttggcgatt attacatttc ctgtaactgc aaggacacgg
    34801 atgatgaaga tatgaggaat atagacttgc tgattttcta ctgtcttgaa cggtattagg
    34861 actgaatctg ctgcgcggag tgcggcaaga gcgaccggag ggagtacaca caggccctgc
    34921 ctgtgtgaaa agccataccg caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga
    34981 ggcttggggt gtccccaaca agcaaaacga gaacgccgca ggcgggcaag tggtggatca
    35041 gaatatccgt aaaggatatt ctgatccatt gcttgccagt attatcggga tgttttgaca
    35101 accgctgtaa tcgaaacatc ccaaacatta agaaggtgtt aattattatt atcttgattt
    35161 aattcgttta aaccaataat atagatacat acaccatgaa aagaagcgag tatcccaatg
    35221 acggatgatt aaaatccgaa aagaaatcgc tttgaaacta cagggaaact acagactgtt
    35281 atgttatctt cttaaaatgg agggattttt atgtcgatac gaattctact tgtcgaggat
    35341 gatgatcata tctgtaatac agtaagggcg tttctggctg aggcaggata tcaggtggat
    35401 gcctgcacag atggaaatga ggcatacacc aagttttacg aaaacactta tcaactggtt
    35461 attcttgata ttatgctgcc cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg
    35521 aaaaatgata ctcccattct gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg
    35581 gcgtttgatg cagaggcaga cgactatgta acaaagccat tcaagatgca gattttacta
    35641 aagcgggtgg aagccctgct gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc
    35701 aggctgacac ttctgccgga ggatttcacg gtactttgtg acggtacaga gctgcccctg
    35761 acacgaaaag aatttgaaat ccttttgctg ctggtgcaga acaaaggcag aaccttaacg
    35821 catgaaatca ttttgtcacg tatatgggga tatgactttg agggtgatgg cagcacagtc
    35881 cacactcata tcaaaaattt gcgggccaag ctgccggaaa atatcatcaa aaccattcgc
    35941 ggtgtaggat accgattgga ggaatcataa taatggaaag aaaagggatt ttcattaagg
    36001 ttttttccta tacgatcatt gtcctgctac tgcttgtcgg tgtaacagca acactgtttg
    36061 cacagcaatt tgtgtcttat ttcagagtga tggaattaca gcaaacagta aaatcctatc
    36121 agccattggt ggaactgatt cagaatagcg ataggcttga tattcaagag gtggcagggc
    36181 tgtttcacta caataaccaa tcctttgagt tttatattga agataaagag ggaagcgtac
    36241 tctatgccac accaaatgcc aatacatcaa atagttttag acccgacttt ctttatgtgg
    36301 tacatagaga tgataatatt tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt
    36361 atcaagggct gacaattcgg ggaattgtta tgattgcgat aatggttgta ttcagccttt
    36421 tatgcgcgta tatctttgcg cggcaaatga caacgccgat caaagcctta gcggacagtg
    36481 cgaataaaat ggcaaacctg aaagatgtac cgccgccgct ggagcgaaag gatgagcttg
    36541 gcgcactggc tcatgatatg cattccatgt atgtcaggct gaaagaaacc atcgcaaggc
    36601 tggaggatga aatcgcaagg gaacatgagt tggaggaaac acagcgatat ttctttgcgg
    36661 cagcttctca tgagctaaaa acgcccatcg cggctacaag cgttctgttg gagggaatgc
    36721 ttgaaaatat cggtgactac aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga
    36781 tggataggca gggcaaaatc atttccgaaa tactggagct tgtcagcctg aatgatggga
    36841 gaatcgtacc catagctgaa ccgttggaca tagggcgcac ggttgccgag ttgctgcccg
    36901 attttcaaac cttggcagag gcaaacaacc agcggttcgt cacagatatt ccagccgggc
    36961 aaattgtcct gtccgatccg aggctgctcc aaaaggcact atccaatgtc atattgaatg
    37021 cagttcagaa cacgccgcag ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa
    37081 aatgccgcct ttttgttttg aacatgggcg ttcacattga tgatactgcg cttccaaggc
    37141 tgttcacccc attctatcgc attgatcagg cgcgaagcag aaaaagtggg cgaagcggtt
    37201 taggacttgc catcgtacaa aaaacgctgg atgccatgag cctccagtat gcgctggaaa
    37261 acacctcgga tggcgttttg ttctggctgg atttaccgct cacatcaaca ttgtaaatat
    37321 ttaaaactta aatgattttg accgacaggt ataaccctgc cggtcttttt ttgctgctac
    37381 aggaaaacta cagactggct acagggaaag tacagatgcg cttgccataa taacaatcgt
    37441 accagccaca aatcgtagtt ttattgcaaa ggaggcattc gatcaaatgg aaaaaagcaa
    37501 ctatcattcc aatgcggatc atcacaaacg gcatatgaaa caatccgtgg aaaagcgggc
    37561 ttttctatgt gcgttcatta tttcgttcac agtctgcacg ctgtttttgg ggtggagact
    37621 ggcttccgta ttggaggcaa cacagatacc gcccatccct gcaactcata caggcagcag
    37681 cactgacgta gtggagaatt tggaggaaaa cgctcttgcc accgccaaag aacagggaga
    37741 tgaacaggaa tggagcctga ttttagtgaa caggcagaac cccatccccg cacagtacga
    37801 tgtggagctt gagcaactat caaatggtga gcggatagat attcggattt ctccctatct
    37861 tcaagatttg tttgatgccg caagaactga tggagtttac ccgattgtcg catccggata
    37921 ccgaacaaca gaaaaacagc aagaaattat ggatgaaaaa attgccgaat ataaggcgaa
    37981 aggctacacc tctgcacagg ctaaagcgga agcagaaact tgggtggccg tgccgggaac
    38041 gagcgagcat cagcttggtc ttgctgtgga tatcaatgcg gacggaattc attcaacagg
    38101 caacgaggtt tatagatggc tggatgaaaa cagctatcgt tttggtttta ttcgtcgcta
    38161 cccaccagac aagacagaga taaccggtgt gagcaacgag ccgtggcatt accgatatgt
    38221 tggcatcgaa gctgccacag agatgtacaa ccaaggggtt tgccttgagg aatatttaaa
    38281 accagaaaaa tgagaaaagg atgttatgtt atggacagaa aaagattgac acagcgtttc
    38341 ccgttcctgc ttccaatgag acgggcgcag agaaaaatgt gcttttatgc gggaatgaga
    38401 tttgacggct gtcgctatgc gcagacgata ggggaaaaat cgctttccca tttgctcttt
    38461 gaaacggatt gcgcattata caaccacaat accggatttg acatgatata ccaggaaaac
    38521 aaggtgttca acttaaagct ggcagcaaag accttaaacg gcttattgat aagaccgggg
    38581 gaaacctttt ctttctggtg gctggtacgc catgcggaca aagatacccc ctataaagac
    38641 ggccttacgg tgaccaatgg taaactcacc accatgtcgg gcggcggtat gtgccagatg
    38701 agcaatttac tattttggat gttcctgcat acgccattga caattatcca gcgcagaggt
    38761 cacgaagtaa aggagtttcc agagccaaac agtgacgaga ttaaaggggt ggatgcaacc
    38821 atttcagagg gctggattga tttaaaagtg cgaaacgata ccgactgcac ctaccaaata
    38881 tgggtgacct tagatgatga gaaaatcatc ggtcaggtgt ccgccgacaa agagccccaa
    38941 gcattataca aaattacaaa tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat
    39001 gaatatgccc aggttaaacg gatgcaagtt gccttaggta ccggggaaat aatagattgc
    39061 aagctgcttt atacaaacaa atgcaaaatc tgttatcccc tcccggaaag tgtggatatt
    39121 caggaggaga accaatgaga aaaagtgtgg gcattactgt ttttggatgc gaacaggatg
    39181 aggcaaatgc tttccgcgct ttatcgccgg attttcatat tatccctacg ctgattagcg
    39241 atgcgatatc ggcagacaac gcaaaattgg ccgctggcaa tcaatgcgtt agcgtaggcc
    39301 ataagtccga ggtttccgag gcgacaattc ttgcgctgag aaaggtcggg gtaaaataca
    39361 tttctacccg cagcatcggc tgcgatcaca ttgatacgac tgccgccgag agaatgggaa
    39421 tctcggttgg cacggttgcg tattcgccgg acagcgttgc ggattatgct ttgatgctga
    39481 tgctgatggc catacggggt gcaaaaccca ccatgcacgc cgtggcgcaa caagatttca
    39541 gattggatcg tatccggggg aaagaactgg gggatatgac tgtgggagtt attggaaccg
    39601 gccatatcgg gcaagcggtc gtcaaaaggc tgcggggatt tggatgccat gtgctggcct
    39661 atgataacag ccgaaaaatg gatgcagatt atgtccagct tgatgagctt ctaaaaaaca
    39721 gcgatattgt tacgctccat gtgccgcttt gtgcggatac ccgccatctg atcggtcaga
    39781 agcaaattgg agagatgaag caaggcgcat ttttaatcaa caccgggcgc ggagcgcttg
    39841 tcgataccgg ggcgctggtg gaggcgttgg aaagcggaaa gctgggtggt gcggcactgg
    39901 atgtgttgga gggcgaggat cagtttgttt ataccgattg ctcgcagaaa gtgcttgacc
    39961 atcccttttt gtcgcagctt ttaaggatgc cgaatgtgat catcacaccc catacggcgt
    40021 actacactga gcgtgtgctg caggatacta cagaaaaaac aatcaggaat tgtctcaatt
    40081 ttgaaaggag tttacagcat gaatagaata aaagtcgcaa tcatcttcgg cggttgctcg
    40141 gaggaacatg atgtgtcggt aaaatccgca atagaaattg ctgcgaacat tgatacggaa
    40201 aaattcgatc cgcactacat cggaattaca aaaaacggtg tatggaagct atgcaagaag
    40261 ccatgtacgg aatgggaagc cgacagtctc cccgccatac tctccccgga taggaaaacg
    40321 catgggctgc ttgtcatgaa agaaagcgaa tacgaaacac ggcgtattga tgtggctttc
    40381 ccggttttgc atggcaaatg cggggaggat ggtgcgatac aggggctgtt tgtattgtct
    40441 ggtatcccct atgtgggctg tgatattcaa agctccgcag cttgcatgga caaatcactg
    40501 gcctacattc ttacaaaaaa tgcgggcatc gccgttcccg aatttcaaat gattgataaa
    40561 ggtgacaagc cggaggcggg tgcgcttacc taccctgtct ttgtgaagcc ggcacggtca
    40621 ggttcgtcct ttggcgtaac caaagtaaac ggtacggaag aacttaacgc tgcgatagaa
    40681 gcggcaggac aatatgatgg aaaaatctta attgagcaag cgatttcggg ctgtgaggtc
    40741 gggtgtgcgg tcatggggaa cgaggatgat ttgattgtcg gcgaagtgga tcaaatccgg
    40801 ctgagccacg gtatcttccg catccatcag gaaaacgagc cggaaaaagg ctcagaaaat
    40861 gcgatgatta cagttcccgc agacattccg gtcgaggaac gaaatcgggt gcaggaaacg
    40921 gcaaagaaag tatatcgggt gcttggatgc agagggcttg cccgtgttga tctttttttg
    40981 caggaggatg gcggcatcgt tctaaatgag gtcaatacca tgcccggttt tacatcgtac
    41041 agccgctacc cacgtatggt ggccgccgca ggaatcacgc ttcctgcact gattgacagc
    41101 ctgattacat tggcgttaaa gaggtgataa gtatggaaaa tggttttttg tttttagatg
    41161 aaatgttgca tggcgtccgt tgggatgcca agtacgccac atgggataac ttcacaggaa
    41221 aaccggtgga tgggtatgag gtgaatcgca tcatcggcac aaaggccgtg gcgtttgctc
    41281 tgcgcgaggc acaaatccat gcggctgcgc ttggctatgg cttgctttta tgggatggat
    41341 atcggccaag aactgcggtg gactgcttcc tgcgttgggc agcgcaaccg gaggacaagc
    41401 tcacaaaaga aaaattttac cccaatatag agcgagccga gttgattaca aagggttatg
    41461 tggcctcaca atccagccat agccgtggaa gcgcaattga tcttacgctc taccacctgg
    41521 atacagggga acttgtttca atgggaagta acttcgattt tatggacgaa cggtcgcatc
    41581 atacagcaaa agggataggg gatgcagagg cacaaaatcg aagatgcttg cgtaaaatca
    41641 tggaaagcag cggatttcag tcttatcgct ttgaatggtg gcactataag ttgattgatg
    41701 agccataccc cgatacctat tttaattttg ccgtttcata atgaaagtat tggttttgta
    41761 tcagattttg gatttgataa aatctatgct ggtcatggac aatcttttac tggaaagtgg
    41821 ttaaagcatc ttttgtaata cattgcaaat tatagtattc aatctgccca gcggcagaaa
    41881 agaaaaaaac cgctgttggg cagacctttc ccacgcccat tttgtatttt gctggcggcg
    41941 gttttgagaa atcaaggccg ccgttctttt ctggcagcaa aaggtgtggc ggcccatagg
    42001 aggatgccgc tatgctgttt tgctattgcc atgctctaat ccacttccac cactcaaaaa
    42061 aagccttgcc cctccacagg gctattccgg ttatgagtat gtactatacc atgtaaatca
    42121 gcagcatagc aaagcatttt ctcgcccggt aggtttgcga cctgccgggc gatttttgtc
    42181 gtcttttgcg gcccggctgt ccccaaaaaa attttttgag aaattttcaa aaaggaggta
    42241 tttagcgggt agctggcggc cttgtcaaag tcttgttagc ggaaagggag agaaccacct
    42301 aagtccccca cagaaagggg gtgagaagat ggatgctatc ccccgcgact atgaagcgcg
    42361 gtgcatgttc gatgcttttt gcaagacggt actgcgtaac gaggccaaaa gctacttggc
    42421 tgaaatgaag cgccgccgtg accgtgaagt gtcactaagc tctttgtcac aggcagattt
    42481 ggacaagctc tgtacagtag atcactaccc cagcgacagc tttacgtttt cgtcgcatgg
    42541 ctatgaccta catatcaaca atgagcttgt tgccgaagcc tttgccgcgc tgcccagcat
    42601 ggagcagagt attttaatcc tgcactgtgt tctggatatg gcagatggtg agatcggcgg
    42661 ccttgtggga atgtcccgga gtgccgttca gcggcacagg acaaatacct tatcggagct
    42721 gcggaaacag ctaaaggcgt tgatgccgaa gggaggttag acgcggatga agaacccgaa
    42781 ctacaagggc attgagcttt tgcccctttc ggtgatcgag gccgcccgcg ctggggatgc
    42841 cggagccgta gagcgcgttt tgcagtatta cagcggttac atgaacaagc tgtgtacccg
    42901 gacgctctat gacgaaaacg gctatccgca tgtgtgtctg gacgaggcca tgaagcgccg
    42961 tctggaaatc aagctcatcc acgctatcgt cacgaactga cgaaccggcc ccggcagcat
    43021 gaaacggctt accctttccc cgtttcggtg ttttcccggt ggcctgatcg caccttgata
    43081 aatatgcccg caagataacg gcggcgcatc atagggcata cggacacatt ctgtctgtgc
    43141 cgagccggac ggctggtgcg ccatgatact tttccctacg ggaaaagcgg agcgaggaac
    43201 accacgccgc aatgtgagcg tggcggcttg cgggcgacga cgtacaggca ggactatgat
    43261 actcccttat agccacagtc cgagcgttaa aagcgtcgca ggcaatgggt agggccgcgt
    43321 gagaaccacg cgggggtgaa agtcccgtgg agctgtgcca gcagccgtcc gtctatgccc
    43381 ctaaatcaca caaacctttg aatttgtgaa agggggctgc attatgaacc atgatgtgcc
    43441 tatttgggaa aaatacacgc tgacgattga ggaagcgtct aaatatttcc gtatcggaga
    43501 aaacaaattg cgtaagctgg ccgaggaaaa tccggcctct ggctgggtca ttatgaatgg
    43561 caaccgcatc cagatcaaac gcaagcagtt tgagaaaata attgacactt tggacacaat
    43621 ctaatagtga aaaagggccg cagatatgtt ataataattt ataagcatat atgcggctct
    43681 ttccgtcatg gaaaggagca tgacgagatg tcagaaaaaa gacgagacag caaaaatcgc
    43741 gttttgcggt ctggagagag ccagagaaaa gacggacgat atgcttacaa atatgttgat
    43801 acctttggaa aaccgcagtt tgtttactca tggaagttag tgcctacgga taagaccccg
    43861 gcaggtaaac gggaggatat ttcactccgg gaaaaggaga aagaaatcca gaaagacctt
    43921 gacgacggga tagacaccat cggcaagaaa atgaccgtct gccagctcta cgccaagcag
    43981 atcaggcacc ggggcaatgt gaagcacaac accaaactgg gccgggaacg gctgatgcgt
    44041 attctggaac aagatcggct ggggagctgc ccgattgaca gtgtgaagct gtccgacgca
    44101 aaggaatggg cgcttcggat gaaagaaaag ggcctttctt ataagaccat caataacgac
    44161 aagcgttccc tgaaagcggc tttttacacc gccatacagg acgattgcat ccgaaaaaac
    44221 ccgtttgact tccagcttag tgacgttctg gacgacgata cggaaccaaa ggtgcctctt
    44281 acaccagcac aggaagaaag ttttctgtcc tttattcaag gtgataaggt ttaccaaaag
    44341 cattatgacg caatcgttat tctgctgggg acagggcttc gcatttcgga actgtgcgga
    44401 ctgacggaca aagatttgga ttttgaaaac cgggtaatca tcgtgagcca ccagcttttg
    44461 agaaataccg gcgtcggtta ctatattgac gaacccaaga cccaaagcgg cgtcagaaaa
    44521 atccccatga atgaggaagt gtatcaggcg ttccagcgag tgataaaaaa ccgcaaaggt
    44581 gcaaagccgt tcattattga cgggtatgcc aatttcctgt ttctcaaaca gaacggctat
    44641 cccatgacgg cggtagatta tggcgggatg tttggcaggc ttgtgaagaa gtacaacaag
    44701 agccatgaag aagcgttgcc gaaaacgacg acgccccatg ctatgcgtca tacattctgc
    44761 acccggttag ccaatgcggg aatgaacccc aaagccttgc agtacattat gggacattcc
    44821 aatatcacca tgacgctgaa ctattatgca cacgccacat tcgattccgc aagggcagaa
    44881 atggaacggc tggcagcctg acacaaggcc ggttttacta ctccatttac tacttttgag
    44941 ggggaaatgg tgagaggtaa taagaacatt tgtaagtatc taacaatatc aaaaatgccg
    45001 gaaaagcccg gaaacacggg ctataccgac attcaagaac ttctaaaaag ataatctaaa
    45061 atcccatata attttatatg aaaacatatc gttttcttgt atgattaaat caccacaaaa
    45121 taaacaactg agagaacgat atgctttatg aatggttcta tcaaaaaaat tctgagatca
    45181 atagacaaaa atttgatgat tacagaggtc tcttacgaaa ccttacagaa gaaaaaaaca
    45241 ttgatcgttg ctgcagttct cttccctgct ccatgtgtct gtagaaattg tggttctgct
    45301 gtggtagatg gaaataggaa attaatggtg gtgaaaaaca agaagaagga aaccattgtc
    45361 cactttgaat aatataatca tatgccaatg gttatacgcc taaaaaagca gcgctatacc
    45421 tgtaaaaact gtcgaagcca ttggaacgct caaagttatt ttatccgtcc aaggcactcg
    45481 atttcaaatc atgtcagaca taaaatcact tctttactga ctgaaaaagt gtcgttattt
    45541 tttatttcga aaagttgtta ggtatctttg acaaccgtta ttcgcacatt gaaagagttg
    45601 agaagctatt taccaaagtc ttccaaaaga attctgtctt gagtgttgag ggttgtgaat
    45661 ttcgttctca cgtt
//
LOCUS       DAYYAO010000252        27290 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.guided.034, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000252 DAYYAO010000000
VERSION     DAYYAO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.guided.034"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(172..2310)
                     /locus_tag="ACHARI_003031"
     CDS             complement(172..2310)
                     /locus_tag="ACHARI_003031"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8443966.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(2349..3935)
                     /locus_tag="ACHARI_003032"
     CDS             complement(2349..3935)
                     /locus_tag="ACHARI_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8443967.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(3925..5145)
                     /locus_tag="ACHARI_003033"
     CDS             complement(3925..5145)
                     /locus_tag="ACHARI_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8443968.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(5157..5963)
                     /locus_tag="ACHARI_003034"
     CDS             complement(5157..5963)
                     /locus_tag="ACHARI_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8443969.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(6173..7453)
                     /locus_tag="ACHARI_003035"
     CDS             complement(6173..7453)
                     /locus_tag="ACHARI_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8443970.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(7497..9530)
                     /locus_tag="ACHARI_003036"
     CDS             complement(7497..9530)
                     /locus_tag="ACHARI_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8443971.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(9564..10415)
                     /gene="rfbD"
                     /locus_tag="ACHARI_003037"
     CDS             complement(9564..10415)
                     /gene="rfbD"
                     /locus_tag="ACHARI_003037"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8443972.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(10513..11541)
                     /gene="rfbB"
                     /locus_tag="ACHARI_003038"
     CDS             complement(10513..11541)
                     /gene="rfbB"
                     /locus_tag="ACHARI_003038"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8443973.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(11563..12135)
                     /gene="rfbC"
                     /locus_tag="ACHARI_003039"
     CDS             complement(11563..12135)
                     /gene="rfbC"
                     /locus_tag="ACHARI_003039"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8443974.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(12149..13015)
                     /gene="rfbA"
                     /locus_tag="ACHARI_003040"
     CDS             complement(12149..13015)
                     /gene="rfbA"
                     /locus_tag="ACHARI_003040"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8443975.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(13115..13822)
                     /locus_tag="ACHARI_003041"
     CDS             complement(13115..13822)
                     /locus_tag="ACHARI_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8443976.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(13827..14654)
                     /locus_tag="ACHARI_003042"
     CDS             complement(13827..14654)
                     /locus_tag="ACHARI_003042"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8443977.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(14654..15454)
                     /locus_tag="ACHARI_003043"
     CDS             complement(14654..15454)
                     /locus_tag="ACHARI_003043"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8443978.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(15447..16586)
                     /locus_tag="ACHARI_003044"
     CDS             complement(15447..16586)
                     /locus_tag="ACHARI_003044"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8443979.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(16791..17984)
                     /locus_tag="ACHARI_003045"
     CDS             complement(16791..17984)
                     /locus_tag="ACHARI_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8443980.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(18063..18269)
                     /locus_tag="ACHARI_003046"
     CDS             complement(18063..18269)
                     /locus_tag="ACHARI_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8443981.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(18262..19404)
                     /locus_tag="ACHARI_003047"
     CDS             complement(18262..19404)
                     /locus_tag="ACHARI_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8443982.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            19916..20551
                     /locus_tag="ACHARI_003048"
     CDS             19916..20551
                     /locus_tag="ACHARI_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8443983.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            20687..20779
                     /locus_tag="ACHARI_003049"
                     /pseudo
     CDS             20687..20779
                     /locus_tag="ACHARI_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            20893..21528
                     /locus_tag="ACHARI_003050"
     CDS             20893..21528
                     /locus_tag="ACHARI_003050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8443984.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            21635..22873
                     /gene="mef(H)"
                     /locus_tag="ACHARI_003051"
                     /gene_synonym="mefH"
     CDS             21635..22873
                     /gene="mef(H)"
                     /locus_tag="ACHARI_003051"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8443985.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            23100..23519
                     /locus_tag="ACHARI_003052"
     CDS             23100..23519
                     /locus_tag="ACHARI_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8443986.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            23524..23763
                     /locus_tag="ACHARI_003053"
     CDS             23524..23763
                     /locus_tag="ACHARI_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8443987.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23988..24911)
                     /locus_tag="ACHARI_003054"
     CDS             complement(23988..24911)
                     /locus_tag="ACHARI_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8443988.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(24943..25707)
                     /gene="map"
                     /locus_tag="ACHARI_003055"
     CDS             complement(24943..25707)
                     /gene="map"
                     /locus_tag="ACHARI_003055"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8443989.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            25865..26305
                     /locus_tag="ACHARI_003056"
     CDS             25865..26305
                     /locus_tag="ACHARI_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8443990.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(26483..27052)
                     /locus_tag="ACHARI_003057"
     CDS             complement(26483..27052)
                     /locus_tag="ACHARI_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8443991.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
BASE COUNT         8264 a         5499 c         4708 g         8819 t
ORIGIN      
        1 aaatttgaaa agcaattttc gtgaatttct tcttatttct cggagttaaa cacttctgtc
       61 ccaacctcga cttatttttt tgagtcggca ccttctgcct ttttttgaca aaaaactata
      121 aaaagccgtt atgtgcgagc gaatcacaac ataacagctt tttattatag tttaatctgg
      181 tctcaacgaa aagtcgccga ttccttcttt tgttaaatta tcattgtaaa atggatcatg
      241 cgcaatatat gctggccatt tatcttgcat ttttttgatt tcaccagcaa aacgtctttg
      301 tttttctgga gtatcttcat aacctcttga tttagattcg aaatggtaca attctgcttg
      361 atgtgcatag acattgtaac gacccagttc ataaactttc aagcacaagt caacatcgtt
      421 gaatgctact tctaatgttt catcaaaccc gttaacagca ttgaagtcag ctgccttgac
      481 catcatacaa gctgcagtga ctgctaagta attgacgtca atgaccaatc ggccgaaata
      541 accacaatgt gttcgatcat agttattcaa tgcatgccct gcaacaccac cgattcctaa
      601 taatactcct gcatgctgcg ttgtatcatc tggataaaac agttttgctc ctacacagcc
      661 gattcgatca aactgtgcat aagaaaccat caccgtcatc caatctggct caatcacttc
      721 tgtatcgtta ttcaagaata aaaagtattt gccgtttgct ttttctgctg ctaaattgtt
      781 aattcgagaa taattgaatg gaatgtcaat caattctacg ataaagcggt ctttcagctg
      841 atgcttgtat tctgcaaata attcttgcat ttttggatct gtacttccgt tatccgcaat
      901 aatgatttca taatttgaat aagacgtttt ttcgatgatc gaatccacac aagtttttag
      961 atcttcatag ccattcttcg ttggaatgat cacactgacc aaatcctctt gcaagacgtc
     1021 ataagcaatc tcataaaatc cagaaattct gccaggacgt acgcttcctt tgatgtttct
     1081 tctagacagg gcatctgtca atgctttcac tccggaatca tagatataac ttttggcttc
     1141 gccattactt gcagttgatc ctgggatcgt ccgccaatga tacaagactc ggtcaatgtg
     1201 gtaaatatga tctgcaggga tttgttctgt cacacgaagg accaaatcat aatcttgaga
     1261 accttcatag cctttacgga aaccgccaag ttctttaaca atgcttgttc gatatacgcc
     1321 taagtgagag atataattat ttcccatcaa tgtatcaggc gaccagtctg ctttgaaatg
     1381 cggatcaaag cgattgccat ctgcatcgat tttatcttcg tcactgtaga tcacatccag
     1441 ttctggatgt acgtttagca cttttgccac ttcatataat gcaaatggcg gtaattcatc
     1501 atcattatct aagagtgcta taaattctcc ttctgctatt tccaatgcag aattagttgc
     1561 cagactgatg tgtccatttt cttttcggaa aacgactttg attctatcat cttttgcttg
     1621 ataggattcc agacactttc ggatagctgg atcagtagaa gcatcatcac tgatacatag
     1681 ttcccaatga tcatacgttt gatccagtac cgaatcaatg catttttcga gccatttgat
     1741 ttcaacgtta tagacaggca taagtatgga aatcaatgga cggtacgcaa atccttgtgc
     1801 ttctttcctt tgctctttca aatctggttt ctcatgacga ctgatccact ctgcatagtc
     1861 gatcgatcct ggcttcaatt ccaatttgac ccgacggatc gcttttttta cgccatgagt
     1921 ccttgcataa tttatccctt ttttcagtaa tctttttttt ctttcccatg aagattcaat
     1981 gccgtcatca taagggtatt taccgtttaa tttcacagct atgccatttt gatgtctcgc
     2041 tgtttggaag tctaagatgg cttttcctct cattttccct gataaacgta ccttaaaacc
     2101 gctttgggtc ttaacatcaa gattatataa atgattgata tccaatctta cgacatgtgt
     2161 tgcttcagca atcacatttt ctttttctac ctttatcgta gggatttctt tggtcacttc
     2221 atcaattgcc cagcccacaa taagcaaatc gccagtggcc cgttcacgag tgatttgatc
     2281 gataaatacg gcaatttctt ttttttgcac gattcgttta ctccttatct ttataagtta
     2341 atcattattt acatttcaat gatccatgat tggccatttt gagctactgc ttccactaag
     2401 aatgaacgtt cttgctcgca aaggattcgt aactcaaatc ccgcttctgt tccttttgcg
     2461 agctgttctg cttgattgac ttcttctcgt gaaacaggaa ttgcttcgaa aaatggcgat
     2521 tggtcagctg ataacttata ggacaagggc tggcgatccg aattagcgat tccccatccg
     2581 atgattttta atatttttgt ttctgcatcc cagattttct cgtcaataca tatagaaagt
     2641 tctttttctt ggattttttt cgaagtttct ttcttacgtc gtcctagtct atgttttatt
     2701 gtccattcat cactttccaa caatgtgcaa tagcgatttg tggttgtttt taacgtatgg
     2761 tctagtcttg tattttcctc aagaagatga tgattttctc tttctaattc gtcatttttc
     2821 tcagaaagct gctttgtgac ttcagccatg ggacgaatcg tttccaacaa ttctttcatt
     2881 gttggtgtca attccccgat aaagcgatag tgacaatgga tagtcacttc tttcccagaa
     2941 atatccgtta gaacgaactc tggtaattct ttcccatcaa acaaataaca atcatttact
     3001 gttttgacta cagcattcga atcgataaat gttagttttt ctccggcagc ttctgcgctg
     3061 aattcgataa agctaggttg ttgcgcgaag cgaaacacca ttctttctgt tgtttctgat
     3121 acggggaaac ttagtgtctc attttcccct gtgaaattat tgaatggtat ttgttggatt
     3181 gtttcatctt gtttcgtttg ctgggttact tctaacactt ttacataatt agaattttgt
     3241 ggctctgcaa tggaactttg tgctactgga tgtttcatca atgagaaaaa gtattggtag
     3301 acttcaccaa atggtcgcat tttgaaatca tagcgtacag cttccggcaa ttcttcataa
     3361 gttgatttta attctgtatc acctacagct aagtacaaat aatcttcaat attaatgaat
     3421 aatcccgctt tttcaaaaac tttttggaag ccatcatgag tataaaaaga attatgggtt
     3481 tcatctagga gaccataaga agcccacggc aattggttat tgaacaaatc gatcatgaca
     3541 gaattatgcg ccaagttagg gaatgtaatc aggatctctc cttcttcatt caaaaattct
     3601 ctgactttct tcaatgtttt tccaggatct accaaatgtt ctaaaacatc cgcaaacaag
     3661 acgtaatcaa atgtctgtcc agcataatag tttgcccact caaaactttc gatatcacca
     3721 taaaatccat cttgagcaaa ttcgctgaca aaatcgaaca attccttatc taactcaaca
     3781 atgctcactt cacattgttt tgctccgatt aaatgtttcg ttaatcggcc gtttccggga
     3841 ccaaactcta aaactttact tcctggtttg atctgtgcgg caatttttcc cacactgctt
     3901 tgttcatcta ggtccatttc aaaatcatat ttcattgcta tgagtccctt cttcccattt
     3961 atggtctaag accacgatgc cttctccgat ataagaaccg ctgacaaaca agttcatgta
     4021 ctttgtctta tataccaaag gaaccgttgc attttcttca aagaccgcaa cgtcaaaata
     4081 atattcacca gctaacaaac ctaacttttc atactccaga taaaagatat tctctccttt
     4141 tttccaagga atcttgatac catctagtag agtattaagt ccgcatacat aaaaattgtc
     4201 gactgttcgt agcgcaattc ctaaaaccgg atttttgatt gtatcatctt ttacttcata
     4261 agttacttta acgtatagtt tttctccttg ttccagcata tccaccggat ttttgtcttt
     4321 atccaacaat tccgctttcg tcacgtcgac gatatctgga gcggcgaacc gttctacttg
     4381 gtttttattc cgatccactg ttttaactga ttttttcttt aaaaagtttt cataattcgt
     4441 cgttacttcc atcgtatcgc catattcgac gacttctccg tttttcaacc aataggaacg
     4501 atcacaaaaa cgacgaatcg aactgatatc atgagaaacg aataaaatcg tctttcccga
     4561 gttcttgatt tctgtaaatt tttccataca ttttagttga aattcaagat cgcctactgc
     4621 caatgcttca tccacaatca aaatatcggg attcacattg attgctacag caaaagctag
     4681 acgaacgaac atcccgcttg aataagtttt tacaggttga taaatatggt ctccgatgtc
     4741 agcaaattca atgatatcat cgaccatttc atcgacttct tctcttgaaa atcctagaac
     4801 cattccattt aaatagatat tttcatagcc agaatactca ggattgaatc ctgatcctag
     4861 ttctagcaaa gctgagattt ttccttcgat ttccatcgtg ccggatgttg gtgtcagtac
     4921 tcccgtaatg attttcaaca ttgtagattt tcccgatccg ttttctccca cgaaaccgat
     4981 catctcacct ttttttattt ctacattcac gtttttcaac gcataaaata catcatgata
     5041 ggatttctta aaaggattga gggcttcacg aaatcgatcc gaaggttttg cgtacatatt
     5101 ataagatttt gtgatatctt ttagtctaat tgcatattct gtcatagcta ttctccttac
     5161 agcacatctg aaaaatgtgg tttcaatcta cggaacactg cagagccgat tacgaacatt
     5221 actaacgtga aaacccagaa atacaaagta tatgttccat gctcccagaa ccaagcttca
     5281 cttaaaaatg actcacgata accttgaaca acatagtaca gcggatttaa tttcaatatg
     5341 cttacaagac ctgaatcaaa ctgattaggg ctccataata tcggtaatgt ccacatgatc
     5401 aactgcagca cgatcgtaat taattgcatg atatcaggta aaaatggttg gatcgatgcc
     5461 gtcagccaag tcaatcctgt caagaaagca cacatgcaaa ataaataata aatcagctgt
     5521 aaagacattg ctgaaggata tataccattt ataagcgcga cagctatgcc aatcagaatg
     5581 aagaataagt gggtgtacag attggatagg attttcgtcg tgggcaagat acgaatattg
     5641 aatacgactt tctttactaa ataagtatat tcccgaaaaa caaccgtagt tgatgcaaat
     5701 gcatctgaga agaagaacca gggaatcatc ccggtaacta aatatacgat aaatgggtat
     5761 tttgcatcga tcatttgtgc tttcagtcca acaccaaata ccagccaata aatcaataca
     5821 gtaaatagcg gattgataaa cgcccataag attcctaaaa acgatcctgc atatcttgat
     5881 ttgaagtcat tgatcgaaaa ctgtagcaat aattttctgt tctgataaat gtccttcata
     5941 aataaataca aatctttaaa catttgtgtt cctccaagcg attttcagtg aattacagtc
     6001 aagtgccatt ttaccatatt tttggtaccg agctgcgttc ttatttcttt cttaattttc
     6061 tatgatacaa gattttctct aacgtttcta ttttacagaa gatgctagac aattgcaata
     6121 aaccaaaaaa ggctgcgacg gttgtcacag cctctctcgt attttgtgcc tatcaatgat
     6181 agcgttggat cctggaagaa tgagcgctat ctggttgttc ctcataagat gtatcatcat
     6241 aagtcatatc gtcatatgcc tcttccataa catagaattc ctccgcaact tctggttcat
     6301 catatcgatg aacggccata gccaacagga tataaataat cggaacccat gggtatgaag
     6361 tcaatgtatc ctccataaat gactgcatca tcaccgcaaa aaaggcccag ataaaagtca
     6421 tctgcccgtt ggataaatct gctttcaacg ttaagcgacc aataaaaagg ataagcagca
     6481 aagcagcaat cccaaatcct atcaatccat agatattcaa aatagaatta taactattgt
     6541 ggtttcctaa aaattggttt cctcgttgga agataaatgt tttcattcct agtaaaattt
     6601 gttcagattt ttctcctagc gtctgataga atttatgcca aatatcttct cgaccggtaa
     6661 acaaattgac tttttcagag acagccgcca gataagaaat cggcaaagct aaaagaatcg
     6721 taaccgtgta agcaataaat ggcgcacgaa tgacacgcat caacgattta ggtaaaagat
     6781 ccaatatcgc aaaaacaatc aacgcgacta atgcacctct agattggcag acccaaatcc
     6841 cgagaaaagc tgcaacaact gctactgcac cgatgattcg tacataccat cgttcaaaag
     6901 aactggcaaa tccactgatc aataaagcaa gagtcatcaa agaacttccg attatattcg
     6961 tattgatcca aatatcagaa agcgaattgc ctggagccca gatgctttgt ccttctggta
     7021 cttggatctc tgtaaataca cgatataacg tgattccgag gaagaccgaa aagacaatca
     7081 gataaatacc gcgatcccat tttgtccact cgatccattt aattactaaa caaaataaaa
     7141 taatcaaagg aaacagtaag tctccaaata aagactcatt tcttaattcc cgaaagtaag
     7201 aaaagaaaat gaaaagggca agcgcaacac ctagataaag aaaatcgacg ccttttgctt
     7261 tataaacatt taaaatgagc aaaatagcca cactaaaagc gataagtaat gaactattgt
     7321 tatatataaa caaagttgct ggaaaaccta accctgctgt ccaaacaaaa aagactagcg
     7381 tcagtaacaa aagcaagaaa ccgacactca ttttgttgac aaatttttgt acgattgctt
     7441 gaattatctc cactctcctt tctactaaca tttcaggatt attctcagct agcctattac
     7501 tgtacgacta gctgattttc ctgagtttgt gtttgtggta catctggata atccggatat
     7561 tctactttaa aaatcatata accgtcttta tccggtccat atagctgcgt gaactgcgct
     7621 tcatataatt ggttgtaatc agttaatggt cgtttactga tcacatagtc gatccctaat
     7681 ttttctgcgt cttcaaaatt caacgtgacc gaaaacatat ctggacgatc taaaatgaac
     7741 tttgtatctt ccttagcgat aaaagctctt gtatgagcat accgattata atacttctcg
     7801 tattcttctt tcggatcgat tttcttccat gcagctatat ctggataaaa gcgtactgta
     7861 ttgaaggatt tcactcccaa tgttggggtg aagttatata attcatcttc agacaaccaa
     7921 actttaccag gatcttgttg atcgattttt tggatttctt ttgccagtgt tttttcaaaa
     7981 atcgctcctg taccttctac gactggattg acgaaaaagc cagatacgat aactaaggcg
     8041 gcaaaagcga ggtaaaacaa gcgtctccag ccaaataatg caaaagctaa aatcactgtt
     8101 cccaacacag caactgcgat ggtttcaaaa tccgaaaaat aattatccat tttcgaatgc
     8161 gtaatcgcat agaaataaga ccatgcattt actgccaaca aaatgaattt ataccaaata
     8221 ggaataacat cttctctcca aatataagcg ataaaccaga tcgataataa acaagccgca
     8281 aaactaaaag taaggatggc tcttaatgga ggaacataag acagcagagt gatttctgct
     8341 agccattttg gcaacccaac ggtaatccaa aagatagaaa acagacaaaa aatcatcaaa
     8401 atacgtccca gtaacttttg ttcgctatct tttttgccaa ataacacaaa tggacttgct
     8461 aggaatacag ttggaaaaaa gtgatagaac agggccactt cagagttatt cgaatagttg
     8521 atatcttcaa aaggaatttt ccaatttgta aggaaagaaa aaaattcgcc aatcgtccac
     8581 tttcctccag tacttacccg ttttcctgga tataaggtat tcaaagaagc taacaatgcg
     8641 tcttttgacg ttagccagaa atgaaccatg actccgccaa taaataaaac tgcccctcca
     8701 atcaacacta catccaacag atcccatgaa actttcttgc tatagtaaat aagcaggcca
     8761 acaaaataaa aaatcagcaa gtacgccagc atcacttgat gggctgggta aatcactaaa
     8821 ataaacccaa taccgaaaat aaccaccaaa agcatcatca acgttcgtag ccatttttta
     8881 tcatgattgt agaaataatg ataaaaagca gtcatcagtc ccaaagtaaa gaaaatcaaa
     8941 tcgcccacat gttgcatgaa ccaccactgg actgctggag aaaatgtcag cgcaaatgca
     9001 ccgattagcg atagagcttt ttttctcttc gttaatatca tcagtacttc gaatccaagc
     9061 aagaccatga cgactaattt catcgcccaa taaaaggaaa gtcctcgatc tcttccgagg
     9121 aaaaagaatc cccagttgaa tggttttccg ataaccgtaa tatcctttac aggagaatta
     9181 tacgcaatga tcatgttttg tccgtttaat gaataatcct cgttcaccaa cggaaagtcc
     9241 tcttcagctt gtgagaggta aaaaggtgtc tgcaccagcc attcgtctga ccgcacttcc
     9301 cgattctgtc caaaaatcac atcagttttt ttcccatcgt ctctttgcga cacatattta
     9361 tcccaaacac caagtgaact tccgtttaaa ttaaaagtaa cgatgaccac gatgactaaa
     9421 acgccaataa gatagcggag ttttagaaac aggtcaagca ccaaattcga atctgcctgg
     9481 ttcgcttttg gcttttcttt tcgtcgtgca cttcttgttt gtttttccat atcatgctcc
     9541 aaattctatt tgataaaagg aaattactta ttcatttttt tggcagcttc cagcatttgc
     9601 gcaagtgctt cttgccaagt tggtatgaca aatcctaact cttctgtctt tttcaggctc
     9661 atgactgaat actgcggacg tgttgctttt tgcggaaatt cttctgacgt aacaggtgca
     9721 acttctacat ctgtatcttt caatatttct tttgcgaatt cataccaaga acagctattt
     9781 tcgttcgaca aatgataaat accaaatggt gctttttctt cgatgacata taccataaat
     9841 tctgccaaag tacgtgtcca agttggtcgc ccaaactggt catttactac agtcagtttg
     9901 tcgcgggttt ccgctaggcg ttgcatcgtg aagacgaaat tgtgtccata gatcccgaaa
     9961 acccaagaag ttcgtatgat ataatagtct tctaaaatct cttctactgc tttttcgcct
    10021 aataatttcg tacgaccata ttcatttagt ggattcgttt ggtcgtctac ttcgtattct
    10081 acatttttgt tttttccatc gaaaacatag tctgtactga tatagacgaa ttgagcattc
    10141 acagcttttg ctgcaagtgc tacatttctt gtaccatcaa cattgatctt ctcatctaat
    10201 tcttttgctt cttcttctgc tttatctact gctgtgtaag ctgcacaatg gaaaataaca
    10261 tcaggtttta attctgtgat gtaagccatt gttttctcgg catctgtgat atccaagtct
    10321 tttgcatctg ttgaaacata ttgcagattt ttttcatcta ataaatggcg caattctgta
    10381 cctagttgcc catttcctcc agttaataaa aacatatgat tatccgctcc ttgattttta
    10441 ttttaaaaaa gagacaggcg agcgcctgcc tcacatgtgt ttttccatta atacagtgac
    10501 attttctctg tattattgtc catttttggc atatgctgct tcaacggcct ctttttcttt
    10561 tttccaccag tcttcatgtt cttcgtacca tttgatcgtg tcagccaacc catcacggaa
    10621 gttcgtaaac tcaggcttcc atcctaactc ttcccgtaat tttgtcgaat cgattgcata
    10681 acgcaagtcg tgtcctgcac ggtcgtttac atggtcgtac gcatcttttg gttgacccat
    10741 catttctaag atcaattcca tcacggtctt attatcttct tcaccgtctg caccgatcag
    10801 ataggtttcg ccaattctgc ctttcgtcaa aatcagccac actgctgaag aatgatcatt
    10861 cgtatggatc cagtcacgaa cattctttcc ttcgccatat aattttggac gaataccgct
    10921 taagatattc gttacttggc gaggaataaa tttttcaata tgctgataag gaccatagtt
    10981 gtttgaacag tttgaaatgg ttgcacgtaa gccaaaagaa cgcacccatg cttttactaa
    11041 cagatctgaa ccagcttttg tagaagaata tggacttgaa ggattatatg gtgtttccgc
    11101 agtgaatttt tctccttcgc cttccccatg tcctggcagg tcttcacgta atggaagatc
    11161 gccgtaaacc tcgtcagtag atacgtgatg atagcgtaca tcatacttgc ggcaagcttc
    11221 taaaagtgta tacgtaccaa tgatattcgt ttgtacaaat gggaaaggat cttttaatga
    11281 attatcgtta tgggattctg ccgcgtaatg aacaactgcg tccgcttctt ttactagacg
    11341 gtccactaat tctgcatctg caacatctcc tacgaccaat tctacgcgat cgctaggaag
    11401 tccagccaag ttctctttgt ttcctgcata agtcagttta tccaaaacag tgacatgcac
    11461 ttctggatga ttgtttacca cgtaatgaac gaaattagag ccgatgaatc cggcacctcc
    11521 ggtaacaatg atgtttttca taagaaaatt tctccttaat tcttagattt ctccgtaaac
    11581 aaatggattt tctgcttcaa attcttttaa tgtcggatga cggtgatctt tttccgacat
    11641 aatcgctctt gatggatcaa ttggccaatc aatcgctaaa tctggatcat caaatgcgat
    11701 gcccccatct gcttctgcat tatagtaatt atcacactta taaaggaagt tcacatttgg
    11761 tgtcaaagta acgaatccat gggcatatcc tttaggtaca agcaactgtc gatgattatg
    11821 ttcagataag ataaatccgt cccattgacc ataagtcggg ctacctttgc ggatatccac
    11881 tactacatct aaaactgctc cagtgactac ccgaatcaat tttgtctgtg cagcttctcc
    11941 tttttggaaa tgaagccccc gaaggacacc agcttcagct gatagtgaat ggttgtcttg
    12001 aacaaagtca tatttcaatc catgttctgc aaattttgct tttgaataac tttctgtgaa
    12061 gaaaccgcga tgatcgccaa agacgtccat ttctacgatt ttgacatctg ttaattttgt
    12121 atctgttact ttcatctgta aggactcctt attcagcggc caaattcaat aaatattgac
    12181 cgtattgatt ttttttcaat ggctgtgcaa gggccagcaa ttgttcctta tcgatgtagc
    12241 ccatacggta agcaatttct tccaaacaag ccacttttaa gttctgacgt ttttcaattg
    12301 tttcgataaa ggtagatgct tcaagcaatg attcatgtgt tcctgtatca agccaagcaa
    12361 atccgcgtcc cataagttct acggatagtt ttcctttatc caaatatgct ttattgacat
    12421 ccgtaatctc tagttctcca cgttctgaag gtttgatacc tttagcgata gaaattacct
    12481 cattgtcata aaaataaagg ccagttacag cgtaatttga ttttggctga gcaggctttt
    12541 cttcaatcga caaagcttgc atattctcat caaattctac tacaccaaag cgttctggat
    12601 cattgacatg atagccaaat actgtcgcgc ctgtatcttt tgaagctgct cgttgcaaca
    12661 ttttagacag accgccacca tagtaaatat tgtcccctaa taccaagcag acactatcat
    12721 ctccaataaa ttcttctcca atgataaacg cttgtgccaa tccatctgga ctttcttgta
    12781 ctgcatattc gatctgcagt cctaaatcat ttccgtttcc aaataattcc ttgaaacgtg
    12841 gtgtatcttg tggcgttgaa ataatcagaa tttctttgat tcccgccaac atcagagtag
    12901 acattggata ataaatcatt ggtttgtcat aaatcggcat caattgtttc gatgttgcct
    12961 ttgttaaagg gtacagacgt gttccgctgc ccccagcaag aatgattccc ttcatggata
    13021 tctgctctcc tttttgattt caaatttcac tttgttatta tctcatcaat tgttatattt
    13081 gtcatggaaa tattaaggat ttaaacatct tttatcaacg tttaaaccat tttcgtttga
    13141 tcaacgccgt aatcagatga acacgccaag aaattttttg ccgtaattta gaatgtgttt
    13201 caatctcact cgcgtttaaa tcatgtcttc ggtacagagt taacggttct cttaggaacc
    13261 cagtcttctt tcctcccagc aaaccaatcc acatatcatg catcggcact tctgacggga
    13321 tcggcaaaat atcgggtttt aaagaagacc ggaaagccat accagcacca atatagtgac
    13381 ttcttatcac attattaagc catccttctt tggaatttcg aaacgaaaaa tacgaatcat
    13441 ggatctcttg cagctcgctg tccacaatga ttaaatcaga aactactact agctgatccg
    13501 gattctgctc aaaatagtca gtgatctttt tcacttttgt ctccaaccaa acatcatctt
    13561 ggtctgccaa aaagattaga tctcctttcg tctgctggat cgcataacca aaattagcga
    13621 tcaatccttg acgaggcccc gaaaaaagct ggattcgctt atctttttct gcgtattcag
    13681 ttaatatatc ccatgtatca tctactgacc catcatcaga aatgactact tcatcttctg
    13741 ctgaaagctg agataaaata ctatctaact gctctcttaa atatctgcct ccattaaagg
    13801 tagctataca aaccgaacgc acgaattcac accttccaat tcttaacata tgagcgcaaa
    13861 gtcatccgcc agatatgccg gtttttgacg gtcaagaact gtttagctgt tcttaacaat
    13921 aattgtcgtc ggtgactgga taaatgcctt tgatcatatt catgataaaa tcgattttct
    13981 gcttgtaaaa tagaacggta tcgattactt gaaacttttt catagtctaa aaccgaaaga
    14041 tcatgttcca ttcgtacagg aaggatctcc acagtttttt ccaactgatc cactttccaa
    14101 aacagccaat gatccaaaaa atctaaagaa aaagcaggat taaacccgcc aatctttttt
    14161 agaaatttga cagaccaggc aacacctgaa ttgattgcca tgattcgttc agctgttggg
    14221 ccttcaactt ctactgcttg agctgtacga ttgatataac gatctgaata taggggagaa
    14281 acttgtcggc tcccagaaaa aatcatcgga actgcagcaa cacattcatc tgtaaaggtt
    14341 actttacgca atagttcaaa ataatcgtcg actagcctcg tatcctgatc cagagtgacg
    14401 agacaacgtg ttccttcatc tgcttgagac aacgcaaaat tataagccac cgctaaacca
    14461 ggattttgcg gatcatgatg ataaacgata ttttcttttt caaacagctc attttgctgc
    14521 ctttgaggac tattatcgta aataataagc tgaatttgct tttcgctaag tgctttgctt
    14581 aatggtccat aagctggaga ttgactgaat agttgctgat aaagcacaac gactactaat
    14641 atttctggtt tcatcagaaa aaacgccacc cccacttatt gaaaaatttc atcatagaag
    14701 tcaaaaacac gcgaaacagt tttgcatttc gatgcgcacc cttcccgtaa aaatggataa
    14761 ctgcataaaa gggtgtgtac aataacttgt aacctgcttt ttcaaaactc aaacacaagt
    14821 cgttgtcttc aaagtacatg aagaaacgct catcaaaccc ctttatctct ttaaatttat
    14881 caatatcagc taccataaaa ctacctgaga tcatccgtac ataactatta gcatcagtag
    14941 gcaatcctcg gcattcaaac acagccagtc gtttgtcaaa cttttttttg acccatttga
    15001 aaggcacaaa gcgcaaaacg taatcaaaaa cagccagctg ctcacggatg aggtgctgag
    15061 ttgttccatc ttcattaaga atcttaggtg ctaacatcgc acattccgga tgttctttca
    15121 ataacaagac caatgctgta actgtctctt catccactaa aatatctggg ttacaaataa
    15181 tgccatagcg agaatcgcta gctatcagat tgtgattatg tccataacca aatccggaat
    15241 tttcatcatg aaaatagata tcgatgtagg gatatttcct atactctgtt agtctttttt
    15301 tgtactcggg aagtgaattg ttgtcaaaaa ttcggatttt tactgctgtt tcatctttta
    15361 cagttgaaat aaaattatct agtgtccgaa aaattttatc actgtttgcc gtcacaatac
    15421 taataaggat cggctggttt aatttgttat tcatctatat gacggttcca tttcttcaaa
    15481 tatttctgtt ttacttgcgt acggtactct ttattcccta aaaatcgcaa tgtttttaaa
    15541 ataaactgat gtttctcatt gatcagacca atcaattcaa ataataaaat tgcagcaaac
    15601 aacatggcaa taatcaatat gatcgttccg attcttccag tataattcat gaccatagct
    15661 gtgaaagaaa acatcaaagc catcgcataa atcgtcagga cagcaccttt atgagtaaag
    15721 cctaaactga ttagtcggtg atgaagatgt tttttatctg ctgtagaaaa agaaacacgg
    15781 tttgcttttc tcctaatgat agcaaaaaac gtgtctgtta ccggcactcc catgacgatc
    15841 agcaaagaaa tcgatgaaac aaaagtcacg ttcttcagcc cttgtagtga aaggacggcc
    15901 atcataaacc ctaggaatag agctcctgta tcgcctaaat aaatctttgc cggataaaaa
    15961 ttgtaaggaa aaaaaccgat aatacttgct acaagacaga aaataacaac tggaatatac
    16021 actgtagaag catggaggaa aaaatatcca atgattccta tcgtaattaa accgatcaat
    16081 gaaatccctg ccgccagccc atccaaaccg tcaatcagat tgaccgcatt cgtgataccg
    16141 aagatccaaa aaatcgttac gggaaaacta agccagccta gttggatata tccaataaaa
    16201 ggaagcgtga aggaatcaat acgtatatct gctacaaaat aaataataag agaagcaatc
    16261 aagatcccta tacttttttg tttaggtgtc agctcatatt tatcatcgag cactcctgtg
    16321 actatgatta ctaaagaagc aatcaagatc ggtacaatat acgattgagg gataatttta
    16381 cggaataaga ataaagtcga tataacaaat gaaataaaga taggcagtcc gcccgctgaa
    16441 ggcattggaa ctttatttat tcttctttca tttggtttgt cgaccatacc tgtttgtact
    16501 gaaatgattt tgaatacagg tgttaatatc agtgataata aaattgtcag aaaaaatctt
    16561 ataaccatac taaaagaaac aagcatatgg gcacctactt taattaagag tcataattat
    16621 tataaattat atatgtcttt ttgactatct ttttatatct tctcaaatgt ccgcaaagcc
    16681 ttattttatc ggctctacgg gcattttgct tttgtggtaa acctcgcata tctaggtcta
    16741 tcttcttata ttttccctgt caatcgtggt taaaatcgtg gtaaatactt ctatgcgatt
    16801 agacgctgaa cctctgattt tgcggtatct atggacgcat gagcgtacca gttcattgtg
    16861 atactgatgt ttgaatgccc catgatatac tgtaaatctt tcgggttcat gttcttgctt
    16921 gccagccttg tgcagaatgt atggcgtagc gtatgcggtg taatatgtgg caagggattg
    16981 tccttgtggt gcttgttgta tttctttacc atacggataa ataaggcgtt ataatcaatg
    17041 gcaacttttg gattaccttt cggattgata aacacaaaac cgctgtgtcc gtctacctct
    17101 atcgacttta tctttggtcg gctgttgata acccgttgaa atgcctgtat cgtttctttg
    17161 cttaatggca cttgccttat cccgcttttc gtcttaggcg tttcaatata atagccctgt
    17221 tccttactct ttagtaactg gtggtcgata atcacaactt cattcttgaa atcaatgtcg
    17281 gctactgtca gtccgcacag ttccgagata cgaagtcctg tctttaacag tatcagcaca
    17341 tcatcataat gcttgtgata cacgttgtcc gtcttaataa atgacaataa ggcttgctcc
    17401 tgttcctctg tcaatgcgac tttctctttg gtatcatttt ctatgacttc acttagcttg
    17461 aaatcaaaag ggtttttcct cacataatca tcttgtatgg ctatgtagaa tgacgctttt
    17521 aacgagcgtt tatggttgtt aatggtattg taggaaaagc ctttgtcttt catgcgtaac
    17581 gcccattctt tagcgtcaga gggttttatc gtatcaatgc tcctagcacc taacttgtcc
    17641 tcttttaata accgcatgag ttgttcccgt tgtttctgtg tgcttttctt cacatttgtc
    17701 ctctgtgcgt tctgtttggc atagagttgg caaagcgtca ttttcttgcc tgtgctgtcg
    17761 ataccgtcct caatatcccg tcttatctgc tgttccagtt ctcgaagtga gggtttttcc
    17821 cgttttccct ttggtgtcgg gtctgtgggt gtcaatctcc aagcatacac atattttgtg
    17881 tttcccaatg cgtctacata tttatataag tattttccgt ctgttcgttg gctctctcca
    17941 gtatgcagga tacgaccttt gttatcccgt cttttttctt tcatggtgtc tgctcctttc
    18001 cttgttggaa agagccttga tatgacttgt gttcatcata acacatacaa ggctcatttg
    18061 cattagattg cgtccaattt atcaataacc ttttcaaact gtcggcgttt aatctgtatg
    18121 cggttgccat tcataatgag ccagccagcg tccttgtttt cctctgccaa tcgtcttaac
    18181 ttgttttctc caatgcggaa atactttgac gcttcttcaa tggtaagggt gtatttttcc
    18241 catacgggaa tatcctgtgt attactcata aaacaccccc tcactttcga ttacgtctga
    18301 tatgtccgta cacatctgtt ccacaagcat ttcctgtttt tcagaaaggt gggtacattc
    18361 atcaatcatt ttcatgtagt ttgaacctgt cagcctgtcg atttcctcta tcatcacgat
    18421 tgacggcatg acctgtcttt ccagccattg cagagtgcgt tccatattga caggtacagg
    18481 gttcatttca aaaggtaaac tgtcatggga aatgaatatc tcccacaatg gatagtcagg
    18541 aaattccaca aaatcggtaa tgagataaaa caccagccca tgagggttat acgtcaataa
    18601 cagttcttca actgcctgta ccgccttttt gtcacgcaga cgaatttcaa aacggttgat
    18661 aatatcggta tgtttctttt tcgttgcctg ttccttttgt ttttcataca ggcagaaata
    18721 ttttgtgctt gcctttgagc cgatataaag ggtacttgct aaattacggt tttttccgct
    18781 taattttcca ccttgtacat tttcatagtt tttacagcga cattccgcac caccatttct
    18841 gtatttttca gatagcacag gaatatccag cagaccgcac atatcattga ttgccaagtc
    18901 aaaacgtcgg ataacaccgc cacagtcaag acagcggttt aagaatgaat accagtccct
    18961 gttctgtgcc tgtaagacat attccatgtt gcgtgagcct gcccctttta attctaaaaa
    19021 tacgcccatg tgttcatcat cagaagccat gaccttgatt tccccgtaag cataatgctc
    19081 cttatagcca taatacccat agtcataatg gataaaataa tctgctttca tgccaagcac
    19141 ctttcggata acttccaatg cgtcagtcgt tggaaaacgg acagacagat aatcaacgag
    19201 cagataaaaa ggcttatcgc agatatagta attcagacaa cgcaggataa tgttctgtgt
    19261 ttccttgctg gcgttagctt ttccgttctc aaagcggttg atactatatc ttgatacatg
    19321 ggtaagactt gcaagttctc cttgtgaaag accgctggca agacgtttgt tcttcatttc
    19381 aagtgcaaat tctaaattgt tcattgtatt cctccaatcc cacaatagcc ttgaaatacc
    19441 tgtaaaccac ctttcaaaaa gtgtgatatg tactatcttc ttaaaacctc tcattactac
    19501 tcacaggttt gggggtgtat ggtttttgac ttgtcttttg tgaaatagtg cccccctgtt
    19561 agataacggg ggcacagaca aggctcgcag gctcgccagc cgacaaagca tttcttcccc
    19621 ttgacacaag gtgtcatggc ggtaagcctg agaggcttcc gccaccgtgt cagaaatact
    19681 ttgtcagcaa gttctgtttt gccttttcta acaagttcca tttcaaccga tattcatatc
    19741 tacggattta atcagatagg tatttctttt tgaatgtttt tatatttcat tctattgtgc
    19801 ttattcgatt acaagaacat agtaattctt atatgcttat ttgtcaatat ctttttttgc
    19861 ctttacaaat catattaagc atgatagact tataatagcg aagatataac gagatatgga
    19921 gggttatgca atgaaaataa gccatttagg aaataatata cagaccataa gaaaatttag
    19981 gggaatgaaa caacaggaac ttgcggacaa aatcggtatc aatatgcaga gcctttccaa
    20041 gattgaaaga gggttgaact atcctgccta tgaaacgctg gaaaagataa tggaagtgct
    20101 ggacgttacg cccaatgaat tgttgtcggg agaatggaag tatgtcaatc aatctgaaaa
    20161 ggaagtctgt cagtttttaa gagccgaaga acgtctaaat gcagaactga aacatggaca
    20221 ctatgataac ttctttgaca gcgaggaaga atggctggaa tatgagttag aaaagttacg
    20281 ggaatatatt acagactata tcaacgggaa aagcattgaa gcgtccgacc tttacccaat
    20341 caaagaattt atccagcatt tgaagtttca aaaattatta gaccgctatg atgatttata
    20401 cagtatggat atgtttgggg aaagcataga gggacataag tataagacac cttatcaagt
    20461 cgtgaaaatg ataaacccga actcaaaaga agatatggaa ctgttacgag aagtattaag
    20521 gaataacaat tttgatgatg aggacgagta acttttttcc tcttgcgaga agatacagca
    20581 aacaaaaagc ccgatcaaga gtgcaaagca ccaccaatgg tggcaaagct cttgacaggg
    20641 ctttttcttt tctgttgtgg gcaatcaaga ggaagaaagg gataaaatct gatttgtggt
    20701 aaatcaggca aaggcaacgc aggtatcaca gtatgaattg acaccgcata aggaaagcaa
    20761 ctggaagatt gtagaataaa tgaaaaaggc ttgcattctg catttttata aaattgcaag
    20821 tctttatatt gacaagcata aaatactaag atatacttta atcagaccaa cggtttgtat
    20881 tggaggtaag ttatggcacg aaataaatat ccagaagaaa cagtaaaact cattttagat
    20941 gtttctacaa aaatgttttg tgaaaaagga tatgataaca catcattgca agatattatc
    21001 aatgaaacaa aactttctaa aggtgctatt tatcatcatt tcgattcaaa ggaagatatt
    21061 ttaaaagcaa tttttcaaag aattggaaat gaaaatgcag taatatttgc taaaattaga
    21121 gataacgata gtttaaacgg tttagaaaag ttaagagaaa tatttaagac agcggtattc
    21181 aattctaacc aaagcgtttt attgacagtt tctcctcaac tattaaacaa tcctcgtttt
    21241 cttgctatgc aaatagaaca aatgtatgaa attgtagtac ctgaatttgt tgaacccgtt
    21301 ttagaacaag gtatcaagga taagtctttg gagattgaaa attcacatga aattgcagaa
    21361 gttatcatgg tattaagtaa tgtatggctc aacccattag ttgaaatgac ggataaagag
    21421 ggtatggaaa aacgttgcaa tacattcaat gcccttttaa atggaatagg catagacaca
    21481 ttattagacg aagaaacaat ttctgcattt atacagtttt gtaaataact gtatttgtta
    21541 tccctatacg atagactgct ctataaaaga gcagtttatt taaaccatta tataaaccga
    21601 cggtctgttt gattaaataa aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa
    21661 tcgtgatttt atacttgttg ttataggaca gataatttct ttatttggaa acgctatcct
    21721 acgatttgca ttaccgtact atttgttaaa agaaactggt tcatcagctt tgtttggtat
    21781 tgctacagcc tgctcttttt tacctatggt tattttgtcg ctggtaggtg gtattattgc
    21841 tgacaggaca aataaaagaa acattatggt catactagat tttgtaacag caatcataat
    21901 tttagtattt tatttgtcat tgggaaaaat tcctatcata cctttgttta ttgttgcttt
    21961 gatgttactt tatggtattt ctggtgcata tcaaccagcg gtacaagcaa gtattcctct
    22021 attggtatca tcagataaac ttaccgtcgg taatgctata ataaatcaag taaatacttt
    22081 agcaaacttt atcggaccag ctataagcgg agtagttttt caattttatg gtatcaatcc
    22141 cattttaatt gttagtatta tatgttttac tttttcagcc attatggaaa tttttatcca
    22201 tattccacac caaaaaagac ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt
    22261 aaaagatagt tatacctttt tgaaaaagga gaaacccatt tttatatccg taatcttttt
    22321 ggtttgcatt ttcaatttgg ttttaagtgc tgttttaatt gttggtctgc ctatcttaat
    22381 ttctgaagtt ttaaaaatgc cagatacact atatggtttt tcacaatcag cattatcatt
    22441 aggtggttta tgtggtggta ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa
    22501 cctttatacc ttgttactta tttgttctgt ttctgtttta cttatgggaa tttcgctatt
    22561 tttagaattg cctgcaatag tttcctactg ggtagttaca gtgatgtgct ttgtggctat
    22621 gggtgtatct actatgtttg ttgttcaaat ttacactctt gttcagacac aaacaccacc
    22681 gcaattagta ggaaagataa tggctacgct ggtagcagtt gctatgtgcg gtcaacctat
    22741 tggacaaatg gtttacggta ttttattcga tgtatttata caagaaacat gggttgttat
    22801 gtttgtagct tctattgctt caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa
    22861 gttagagata taatacaagg ggttaaatag tatgttaggc taaaaacaat atataaaaga
    22921 aaatttgaaa tctgctaaaa ggatagtgtt ataaaagaaa gagtaatatt gcagtattta
    22981 aggctcgcac aaatatatgt tttgtgtggg cttttttctt accatttttc tgtatttcta
    23041 aagggtataa acggtattcc gtttatatat ttcagcatta gcagaaaggg ggtgagatta
    23101 tgaggacatc ttctttcgag catatcgtca gattacagtt caattcttta atgctgattg
    23161 tcattaagaa caaagtgaaa agtcgaaaca gacagcttgc aagacattct aaacgtgaag
    23221 tattgttttg tgaaatagcc gaaacgaaac aagttgaacg tggaacgatt gatacctgtt
    23281 tttatgattg tgtatcattt aaagtcttgc actttacaat ttatgtatct gatgaaacac
    23341 ttggtacagt tttacataga ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt
    23401 ttcaaggaat gaacgacaga gaaatttcag aactgtacca tgtatcacgt tctgctattt
    23461 gcagtagaag aactagagga ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa
    23521 gaaatgagta aagattatgg ctatccctct tttgaattga tttgcaaagc gtcaagcgga
    23581 gatgaaatgg cagttaaaga gattttgaag ttttatgacg gttatatttc taaattatgc
    23641 ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta tgcaggttga tgaagaacta
    23701 aaaggagaaa ttcatacaga catgatgaaa gcaatattga aatttgaaat cggagtgaag
    23761 taatcaactg tataaaaaaa tcgggagata cacagtttcg tgctatctcc cttagtagtt
    23821 ttacggtcat atcaaggctc attcaatcgt ggtaaattcg tggtaagatg agtgtttttc
    23881 tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat tagatataaa
    23941 atttcaaata aattactgat ataaatttac aataagaact cttgtttcta ctttttttta
    24001 tgagaaaatt tagttttcaa tttattgaac cattttcggc ttggatctcg acgttttttg
    24061 atttgatgac ccgtcacgta ttcttgaaca actgcattga tgattccgcc caaaatgatg
    24121 atcgtagcag caaaattaag ccaaagcatg agtacaataa aacttcctat gatctgataa
    24181 ccgctgatcc tcgttgcaaa atacctagca tagatactaa acacttgtga taacagcatc
    24241 cagccgacag tagcgaagat cgtgcccgga atgatcgtct tcaacttcaa ctgagcatta
    24301 gggacaatta aataaatcaa gaacatgatg acaaataaag ctaccaatgt gattggccac
    24361 ttcaaagttt ggaactgatc gataaagttc acgggtattt gtacgattgg ctgaatagca
    24421 tccaaaatca gctgtcccaa cccaagaacg atagttaccc cactaatagc aatcatgaac
    24481 aagacaatga caattagtga gaagaaacgg acgatcaaaa aattttttct attttctacg
    24541 ccatacgctt tattcatcgc aatttgcaaa gcattgatac tttgacttgc agaccataaa
    24601 gccgctaatg cagaaacgga taacaagcct cctgaacttt gtgtcaaaag gtctttgatt
    24661 gcaggaccga taaactggta aacggtctca ggaatcactt ctcgtaaata ggtcagtaca
    24721 gtctcttgat cgatgtggag aaacggtaaa atattaccaa aagcaataat caatggaaat
    24781 aaagacaaga gtaaataata agcaacgacg acagaggtcg tactcatttc tgaatcagtg
    24841 atgcgctttt gagtcgtctc aataaagcgc atcagatttt cattttgttt tattttgttc
    24901 caaactttca tgtacctctc ccgtacctat tgactggata gatcaataag tgccttcttc
    24961 tccttgtgat gtcaaaattc ttggaccatc tttagtaata gccaatgtat gttcatattg
    25021 gcaagaaagt ccgccgtctc ttgtatacgc agtccagccg ttaggatcca ttttcatttt
    25081 ccaagttccg gtattaacca taggttcgat cgtgataacc atgccttctt tcaaccgcaa
    25141 cccttttcca gcttctccgt aatgaggaac tgctggttct tcatgaatcg taggaccaac
    25201 gccatgacca ataaaatcac gaacgatcgc tagattttcg ccttctacat aagtttggat
    25261 agcatgaccg atgtcaccaa tccgattccc tacttgtgct tgctcaatac cgatgtacaa
    25321 agctttcttc gttacttcca tcaatcggtc gatctcaggt gtagattctc caactacata
    25381 ggcccaacaa gaatcagaaa tgccgccttt caaatcgata cacatatcga cttttaccaa
    25441 atcaccgttt ttcaaaggct ttttacgagg gaatccatga caaatttcat catttatact
    25501 gatacaagtc gcatattcat acccttcaaa gccaatttgc gcagcaattc cgccatgact
    25561 ctcgatatag tttctgacaa atacctcaat atcccaactt gtgattcctg gttttataaa
    25621 atcacgcaga tttttatgta catctgctaa taaagctcca gattctgcca tcatttcaat
    25681 ttcacgtgct gattttaaag taatcatatt ttcgtctcct ttttacaaat ccctctcatt
    25741 ttaacatagc ggcatacatt tagccaaatg acattcccca ggtgtctcta ggttttattt
    25801 tcttttcaat gatgttttgg tataattcat aattgttaaa taattataaa cgaggaggca
    25861 catcatggct ttagcaaaaa tcgtatatgc tagcatgaca ggaaacactg aagaaatcgc
    25921 cgatattgtt gcagaagcat ttgaaaatct ggatatcgaa gtcgaaatca acgaatgtac
    25981 ccaagtagat gcagatgaat tcgaggaggc agatatttgc gtagtagcta cttatacata
    26041 cgatgatggc gatctgcctg atgagatcgt agacttttat gaagacttgc aagaattgga
    26101 tctattggga aaaatttacg gtgtctgcgg atctggtgat actttttacg atgaattctg
    26161 caaatcggtg gatgatttcg atgctgcttt tgcaaaaaca ggcgcatcaa aaggagccga
    26221 aaatgttaaa gtggatctga atgctgaaga agaagatatc gaaaatttag aagctttcgc
    26281 taaagaacta gtcgcaaaaa tttaatatac ttgcaataaa agagccatcc agatttattc
    26341 aatctgtatg gctcttttat tcattgcatt catggaagag ttcttcggga gttaaaaatg
    26401 catacgctgt aagaaaaacg aacttttcct tattttgaca catctcttgt tacctactga
    26461 tttgtggaac ttttgagtat attcaatcga tttcgattgg ttctttgaat ttcaagatag
    26521 catctatact gtcttggatc atcttttcaa tctgttcatc agttggatgg atgatgcttg
    26581 aagacatcaa agctgcgatc ccagtagcta tgatccatgt cccattatac aaagattcga
    26641 tctgctcttc tgaaagattc acataagctg gatcgcgttt cactgtattc acaaagtaag
    26701 tataagagaa attctgcatc tcttttcctc caccatattc ttctaagtac agtgaacgat
    26761 acaacttctt ctctcttctt gcaaaatgga tataattaaa agcaagatcg ataatcgtat
    26821 tccctgtatg ctcaacagtg aacacttcat attgtaaatg atcatagatc tgctcaaaaa
    26881 gagcttgtct caagtcatcc atatttttaa actcaagata aatcggttgc gtggaacaat
    26941 tcattttgtt cgcgatgttt ctcgcagtaa atcgtgtaaa tccatcggtg gcagctactt
    27001 cataagcagc tgccaaaatc tgttctctag taatcgtctt ttttcttgcc atatttttta
    27061 atctccttaa tttttttata acgcttccac cattgattaa tttcattgta acatgttttt
    27121 ttgatatgca aaattagttt ttgattttct tcacaagctg aaatagcaag tagtaacata
    27181 tatcatgaaa cttaacctta aaaaaataac ctcctttttt tatttaaagc ctcattgttt
    27241 gaatcataat taaatggtat catgctaata gttaccagaa aaaatggtga
//
LOCUS       DAYYAO010000253        18791 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.guided.057, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000253 DAYYAO010000000
VERSION     DAYYAO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.guided.057"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            215..1111
                     /locus_tag="ACHARI_003058"
     CDS             215..1111
                     /locus_tag="ACHARI_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8443992.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1261..2202)
                     /locus_tag="ACHARI_003059"
     CDS             complement(1261..2202)
                     /locus_tag="ACHARI_003059"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8443993.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2328..3449
                     /locus_tag="ACHARI_003060"
     CDS             2328..3449
                     /locus_tag="ACHARI_003060"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8443994.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3707..5209
                     /gene="eat(A)"
                     /locus_tag="ACHARI_003061"
     CDS             3707..5209
                     /gene="eat(A)"
                     /locus_tag="ACHARI_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8443995.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5276..5803
                     /locus_tag="ACHARI_003062"
     CDS             5276..5803
                     /locus_tag="ACHARI_003062"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8443996.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            6014..7075
                     /locus_tag="ACHARI_003063"
     CDS             6014..7075
                     /locus_tag="ACHARI_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8443997.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7078..7974
                     /gene="murQ"
                     /locus_tag="ACHARI_003064"
     CDS             7078..7974
                     /gene="murQ"
                     /locus_tag="ACHARI_003064"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8443998.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7988..9970
                     /locus_tag="ACHARI_003065"
     CDS             7988..9970
                     /locus_tag="ACHARI_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8443999.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10035..10481)
                     /gene="nrdI"
                     /locus_tag="ACHARI_003066"
     CDS             complement(10035..10481)
                     /gene="nrdI"
                     /locus_tag="ACHARI_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8444000.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10692..12116
                     /locus_tag="ACHARI_003067"
     CDS             10692..12116
                     /locus_tag="ACHARI_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8444001.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12297..13070)
                     /locus_tag="ACHARI_003068"
     CDS             complement(12297..13070)
                     /locus_tag="ACHARI_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8444002.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13067..13972)
                     /locus_tag="ACHARI_003069"
     CDS             complement(13067..13972)
                     /locus_tag="ACHARI_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8444003.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14126..14446)
                     /locus_tag="ACHARI_003070"
     CDS             complement(14126..14446)
                     /locus_tag="ACHARI_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8444004.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14920..15606
                     /locus_tag="ACHARI_003071"
     CDS             14920..15606
                     /locus_tag="ACHARI_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8444005.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15608..16552
                     /locus_tag="ACHARI_003072"
     CDS             15608..16552
                     /locus_tag="ACHARI_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8444006.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16751..17395
                     /gene="pcp"
                     /locus_tag="ACHARI_003073"
     CDS             16751..17395
                     /gene="pcp"
                     /locus_tag="ACHARI_003073"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8444007.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17474..18751
                     /locus_tag="ACHARI_003074"
     CDS             17474..18751
                     /locus_tag="ACHARI_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8444008.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6034 a         3314 c         3991 g         5452 t
ORIGIN      
        1 caatatttaa caatgactaa tgataacaaa ttttcagaca attacaacta ttcagaaaac
       61 ttttaggcga attttaatag aagaatgatt tatttcatca tattttcata caaatgttaa
      121 attttcatac aaatatagga aacgttttac tggcttttta ctatttaaac aggctatcat
      181 aggaaaaagt gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg tactgctagt
      241 tgcaaaaaat aaatcacttt attttttgcc ttccatccgc tctttagatg tttggtccgg
      301 aaaagattta ctacagtcgg aattcattaa atcagctaga caacagttgg caaattatca
      361 gctgatcgtt tttttagatt atggatttga tatggagatg gctcgacgcg tgcgcccatt
      421 taccaatgca aaaattgtgt tatttttttg gaatcacttc aaagcagaac acgaggcgat
      481 gctgaagcag gcacaagatg aaccggcaat cgatgagatt tatcattttg attttttgga
      541 agcaaaagaa cttgggctga aacataatag ttcattttat tcgaaacata tgaatttttc
      601 ttctagcgaa ccaaagacgg atctattttt tggtgcaacg aataatggga gaaaagaacg
      661 agcagaaagc tataagcagg aatttataaa aagagatgtt acgaccaact atttcattct
      721 tccacatcga gggaatgaac aacctggata tcttacttat gaagagtacc tgaaaaaaac
      781 aggggaatcg cgcgggattc tggaactgtt gcgggagggt cagcaaggag tgactttgcg
      841 gacatttgaa tccatgtatt ttcaaaagaa actagtgacg gataatcaag cgattgctca
      901 ttatcatttt tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt tagatgaatt
      961 accggaattt ctgcatacac catttaagcc aatcctatca gagctattgg aattttatga
     1021 tgcagaaaat tgggcaaaac gatttatcac taacgattcc actatttatg agcaatacga
     1081 atatgacctc tcctctgttg aaaacaaata aaaaacctac attttgaaga gattatgtac
     1141 tgaaacaaac aagcacaatt cttatggcat catgagagtt gtgttttctt tttgatcttt
     1201 tattgggctt aaaaaaagag aaccagctgc tgattcagct gattctcatc agagagcctt
     1261 ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa aggcacaata
     1321 tggtgtagca atatcatatt tttgtccttt tcgccaaatg gcaccattga tgtagtcgat
     1381 ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat gcaacccgat
     1441 gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat ctagtatgat
     1501 tccttctttt gctgcgatag aagcaaattc agatacgatt gttcgaacca tgctctctgc
     1561 cgctttttgc gcgcctagtt cagcaatatt acaatccaaa atcgtgcata aaccattcaa
     1621 tgttccattt acacaagctt ttcgccaaat ggaataacga acgttatggc tatagatcgg
     1681 atttaaattt gcttcttgaa agacttctac aacttttttt gtaaatgctt cgccttctgg
     1741 ttccaagttc tctagttcga tttctccatc acctaacaat gtgacttttc cgggaccagc
     1801 tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggca catatttttc
     1861 cagtacatct tcatgaccta atccatttag taaacacaaa acataagtat tgtcagtaat
     1921 aatggattgg atcgaacaaa acatatcatc taattgattg gcttttgtta atgctacgat
     1981 caagtccacg tgttcgttgc tttcgatgat ttcttctggt gaatagattg gcagttttgc
     2041 gaccacttct ttaccgttaa agtcagcaat caacccattt gtacgaatgg cttcaatgtg
     2101 agcaggccaa cgatcgatca agaggacctc atttccgctt tgatgtagca tcaatccgat
     2161 tcggctgccc attgctccgg ccccagctat tgcgattttc atgttttttc tctcctttat
     2221 tcaatgagtt cccttttagt atatagaaaa atggaagaaa atagcattgt tttgtttgaa
     2281 aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg gatacagatg gatattttgg
     2341 cggcaattga ttcatttaaa ggaagtgctt cttcatttga actaaatcag gcagttctaa
     2401 atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa
     2461 atactgcaga agcaatctat tacgcgctca ggggaacatg gcgtaccatt gatgggatgg
     2521 atctcttatt ccgtcagcga aaaatcagct atttattgac aacttggcaa ggaaaaaaca
     2581 ccgcagtcat cgaatctgca caagcattgg ggttagatct acttgataaa ccaacggatc
     2641 aaactatccg acttgcttcc agttatgcct taggagaact tattaaagat gcactgcttc
     2701 aagaagtaga acaaattatt gtcactttag gaggaagcgg gtgtagcgat ggtggtctag
     2761 gattactcca atccttgggt gcttctttag aagggtacac agaaggaaat cctttattat
     2821 ctactaaaaa acttcatttt gatcgaagaa acgttcttgt ttctaaagta aaattgatta
     2881 tcgctgcaga tgttaccaat ccatatacag gtcaacaagg agctgcttgg caatttggaa
     2941 gacaaaaagg aggaatagat acgacgctag ctttcttaga taaacaagca ggaaagattg
     3001 ctcagcaaat aaaagatttc tgggaggtgg atttagatga tattccagga agtggagcag
     3061 caggcggtat tggtggagcc ctttttttgt tgggagcgaa gatggtttct ggtttcgatt
     3121 tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc atcattacag
     3181 gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt gtagcagtgc
     3241 ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac caagagctag
     3301 aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca ataggtttag
     3361 aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga cagacactta
     3421 gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta tgctatcgta
     3481 atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc gattgatgaa
     3541 agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt ttcgggattg
     3601 gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca tcctctttag
     3661 ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga
     3721 aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg aacaagcaaa
     3781 tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac
     3841 aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa tccatcagca
     3901 agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct attacgtgtt
     3961 aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat tgatgcaaac
     4021 agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga agacaaaagt
     4081 cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa
     4141 tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg
     4201 cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc atgttttagc
     4261 aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg aagaacagaa
     4321 aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag
     4381 gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag gagataaaac
     4441 aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag gagcagtggg
     4501 tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga tggagaccca
     4561 gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt tgacgatgaa
     4621 tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat tagggtatga
     4681 aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg tggcgatttc
     4741 aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg cattcaacgg
     4801 caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg caagccaaaa
     4861 ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag tagactacca
     4921 agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc ataacaagat
     4981 cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt tatcacagcc
     5041 agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca
     5101 attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac atgatcaaac
     5161 cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac ccttgtaatc
     5221 tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga atcacatgct
     5281 ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt gcggacgggc
     5341 agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag aaaaggtagt
     5401 agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag caagagatac
     5461 agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac gattggtaga
     5521 aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc aaaaagcacg
     5581 tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat
     5641 cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca
     5701 taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg aggggttctt
     5761 tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt tttgtgttat
     5821 ttaaataaaa tatttcaagg cactgctttt gcttagcagt gcctttttta ggttatgaca
     5881 attcttttga taatataaac tcaaccttta gaaatgaaat ttaattattt taataaaagt
     5941 attgaaattt tgtttcttta gatatatact ctagtcatag aaatgaagac ggatacagaa
     6001 aaagagggat aatatgtttg gcttttcaat atttatgaac gattctttgt cagaagagaa
     6061 gaaacagtat atccgccaaa tggcatccaa tggctttgtc ggtattttca cttccatgca
     6121 tataccagaa gatgatgttt cggcatatca aaagcgattg atcgaactag gaagcgaagc
     6181 gaaaaagaat aaactagaat tgatggtcga tatttcagga gaggcattgg aacgagcggg
     6241 tttttcatta gaagatctaa cgcctttgaa agcaattggt gtaacaggtt taagaatgga
     6301 ttatcatatt tccaatcagc agattgccga ctggtcacat caattgaaaa tcagtttgaa
     6361 tgctagcacg atcacgccta aagatatcga tgaattgaaa gaagcagaag ctgatttctc
     6421 acagattgaa gcttggcata attattatcc acgtcccgaa acagggctgg acaaagaatg
     6481 gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc cttgtccaag gttttgtacc
     6541 aggagataca gagttgagag gcccactata tcaaggattg ccaactttgg aagaacatcg
     6601 agggatccat cctcttgcag gcgctctaga tctattagca agtaacacgg atattgttta
     6661 catcggagat gcaggtctgt cagaaaacgt tcaggagcaa tttgcttctt tccaaaaaga
     6721 acagacagtc ttgctccata cggaacctgt agatgaagaa ttttacgaat atatattagg
     6781 gaagcatacg aatcggcaag acgatgcaag agatgtcata cgaagcgtag atgcacgttt
     6841 tcgagagatt ccgcctatcc cagcaagaaa tacagctacg cgtatgaaag ggtctattac
     6901 tttagataac gaaaagtatt tgcgctatat gggtgagatc caattaacca aatatgattt
     6961 gccagcggat gaaaaagtga atgtagtagc taaagttatc aaagaagagc taccattgat
     7021 caaccaaatc aaagctggta tgaactatca atttataaga aaagaaggaa gataaaaatg
     7081 agcaaaatca atttagagaa cctaacaacg gaacgcagaa accaagaaac ttttggatta
     7141 gatgagatga gtatttctca agccgttcaa ctaatgaaca aagaagatca gaaagtggcc
     7201 gctgcggttg aaaaagaact gacaagtatc gaaccagtga ttgctaacac gattgagtcc
     7261 tttaaaaaag gtggacgtct gatttatatg ggagccggaa caagtggaag actaggtgtg
     7321 ttagatgctg ctgaatgcgt accgacattc ggtgtagaac cggaaatggt cataggtcta
     7381 attgctggtg gagaagaagc aatgactgta gctgtcgaag gggccgaaga ttcttatgag
     7441 ctagggcaag aagatctaat caagctgaat ttgacaaaaa atgatatagt tattggtatc
     7501 gcagctagcg gtcgtacacc atatgtgatc ggtggattag attacgcaaa atcgatcggt
     7561 gctgtcacag gaacgatttc ttgcaataaa caagccgaaa tcagtaaaca cgcggatttc
     7621 ccaatcgaag tcgatgcagg tccagaattt ttgacaggct ctactcgatt gaagtcagga
     7681 acagcacaaa aacttatctt gaatatgatt tctactatca gtatgattgg tataggtaaa
     7741 gtattcaata acttgatggt cgatgtcaaa ccaacaaatg aaaaactaat cgaacgctca
     7801 aaacgaatca tcatgcaagc gacagaagct gattacgaaa cagctgaaaa gtactttgaa
     7861 gaagcagatc aaaatgttaa gctggctatc gtcatgattt tgacaaatag tgataaagag
     7921 gaagctcaag caaaattagc tgaagcaaac ggatttgtaa aagggacctt atgaggggga
     7981 aaagaccatg gcaaaaaacg aactgacttt ggaagaactg gcaaaacaaa tttataaagg
     8041 ggcaggcggc atgggcaatg tagaaagcat tgtccactgc atgactcgtg tgcgtatgaa
     8101 gatcatcaat gatgatcttg taaatgtctc tgaattaaaa gcgattccag gagtgcttgg
     8161 tgtagtagaa gatgaacagc ttcaagtcat catcggacct gggaaagtga acaaagtagc
     8221 acagcaaatg gttgatttgg caggcgtgaa attaggagaa aaactttctt ctgtaagtac
     8281 agctacagca aattcatcag catctggaaa agaccgtgta aacgaacggg cacaagaaat
     8341 gaaagcggca caaaaatcga aacaaaaacc atctaaaatc aaatcggtat taaaagacat
     8401 ttctaatatc tttgtgccaa tgattcctgc atttgtcggt actgggatcg tggcagggat
     8461 tgccgcagtt ttagcaaact tagtcacagc cggaacactt gatgcagcaa catggcaaca
     8521 atatatcgat gtgatgaaca tcttgaaaaa cgggatgttt agctacctag ttatctatac
     8581 aggtatcaat gctgcacaag tttttggtgc aacaccaact cttggtggag ttatcggggc
     8641 cgttgtgatg ctgacaggga tgaatccgga agcaccactg aaaaacttat ttactggtga
     8701 agctcttgca gctggacaag gtgggatcct aggtgtcatc ttcgctgttt ggctattgtc
     8761 aatcgttgag aaaaaactgc ataaagtagt acctgatgcg gtagatatca tcgttacacc
     8821 aactctttca ttgattgcca tcggtctgat tgaaattttc ttcatcatgc ctttagcagg
     8881 tttcatttct gatggcatgg tcggtgggat caactgggta cttggcgttg gtggagcatt
     8941 tgccggcttc gttctaggca cattattctt gccgatggtt atgttcggac tgcatcaaat
     9001 tttgactcca atccatgtgc aaatgattga tgaaactggt cgtacgctat tgcttccaat
     9061 tcttgcgatg gctggtgcag gacaagtggg tgcagcattg gcattatgga ttcgctgcaa
     9121 aaaagataaa gaattaacag aaatgatcaa aggagcttta ccagtcggga tcctaggaat
     9181 cggcgaacca ttgatttacg gagtaacatt acctctagga cgcccattta ttactgcttg
     9241 tatcggtgga ggtatcggcg gggcagtgat cggaatgatc ggaaatgtcg gggctatcgc
     9301 tattggacct tcaggagccg ctttgatccc attgatctct gacggtaaat ggttaggcta
     9361 catccttgga ttattggcag cttatgctgg tggcttcgtt gctacattct tcttcggtat
     9421 tcctaaagac cgtttagaaa aagaagaatt agcgactgaa acagccacag taaatgaagt
     9481 gcctgaaaaa gtagtacctc aaacttcagc tgaaagtgtg actatcctct catcagttgc
     9541 tgacggaaca gttgtttctt tagataaagc aagtgatcca gtttttgcac aaaagatgat
     9601 gggtgaaggc tactttgtag aaccagaaaa cggacatatc tattctccag ttactggggt
     9661 agtcagctct gtcttcccaa cgaaacacgc tattgggatc acaacgccaa atggtttaga
     9721 aatcttgctt catatgggaa tcaacacagt tgacttaggc ggtacgccat ttgatgtgaa
     9781 agtaacagaa gggcagcaag tgacacctga tacgcttgta gcagaagctg acttagaagc
     9841 gatcaaagca gcaggcaaag aaacatccat gatcgtcttg gtaaccaata tggatcgtgt
     9901 aaaaaacttt gtcttggaaa aaacaggtaa agcaaaagca aaagcacctg tgatggatgt
     9961 caaagcttaa tatgatagaa acaatagaaa cgaaagaaga aactgaaatc tatttttcag
    10021 cttcttcttt tttattattt attgttttct gctaaaatct gataaattcg tgtcacatca
    10081 gatggtgttc cccgtaattc ataatctgcc aagaaaggaa agccaaatgt ttgtgcatat
    10141 tgtttggctg ttagacaata ctgattattg aagtttttat ttccgctacc gactacacca
    10201 agacaaagtt tatgattgtc ttcatgttcc agatattcac gcatcgtttc ggtcaagatt
    10261 tctgtatcac cgttatctaa accatttccg ccgtctaaat aagtaggaac gaaagtaaaa
    10321 aatggttctg tttctttcgt aaaatcacta ttttcatgga tttcttttaa agtaacttca
    10381 ggattgacag gatcttctgc atgcattttt tcagcatatt ctgctagatg tttggcaaag
    10441 gcacgcgtat ttcctgaaat cgatatatat aaaatgttca tggatcatcc tccaattctt
    10501 tccttttagt atagcatctt ttttaagttt tttggtggtg taatttggaa agggactagg
    10561 cgctttgctt gtataatgag aaaaaagccg gtagaattat atttctatta aagtttttat
    10621 taattaagta aaaataaaaa aggtttgtta tactttttga taaaagcggt tttattttgg
    10681 aggatgctta catggataca gagcaacaga aaaaaggaat tggtttaatt ccacttgctg
    10741 ccttagtaat tggttcagcg attggaggtg gcgtatttgg tattatgacg gacatctccg
    10801 gatcggctgg cggcccagct attttcagtt gggtactcgt aggaacagca atgttgatgc
    10861 tctccttgtc gatcaataat atcaataaaa aacgtccaga tttaaaaggc gggatcttta
    10921 gttacgcaga agaaggtttt ggccgtttcg ctggttttat cagcggttgg ggttactggc
    10981 tcacatgctg gttaggcaac gtagcttttg caacattgtt gatgagcgca ttaggatatt
    11041 tcttcccagt atttgaaggt gggcaaaatc tggcatcgat cgtgatgagt acagtctttt
    11101 tatggggata tgcatggcta gtgaatcggg gagtcgaaaa tgctagtttc gtcaatgcta
    11161 tcgtcacatt ctgtaaatta gtgccattat ttttatttat tgtagtagct atcagcacgt
    11221 tttcgatcca acaattcatt gataactttt ctagcgacat tatattgaat gcgaatggac
    11281 agccgatccc gttttccagt caagtattga gttgcatcat ggtgatgctt tgggtatttg
    11341 ttggtattga aggagcctct gtcgtaggtt cacgagcaaa aaaacaatca gatgttggga
    11401 aagcaacgat tctagggata ttcggcttga ttattattta cgtcttggtt tccatgcttc
    11461 catatgggac aatgacgatg aatcagctgc aaacgatcaa ggcgcagcct gctatgggct
    11521 atgtgtttga aatgatggtt ggtaaatggg gagcagtcgt gatcaacggc ggtttgatca
    11581 tttctttagt tggtgtttgg ctctcatgga cgatcttacc gattgaaaca atgggaaaca
    11641 tggctgaaga cggacttttg cctaaagcat ggggaaaact caacaaaaat ggggcaccaa
    11701 cttatgctat cgtattgacg acgttatgta cgaatgtctt tttactttca ttattattca
    11761 ctgacagtgc ttataacttt gcttacacac ttggtacagc agctattttc ttcacgtggc
    11821 ttttcttagg attgtaccaa atgaaacttt cttatcagcg gaaagaatgg gtccaatttt
    11881 tagtagggct tttagcaagt gcctttcaag tatgggctat gctagtcgta gcctttaatg
    11941 aagtaatgct tgttcttgtt cttttcttgc caggcattta tttttacctc aaagcacgta
    12001 aggagcaaaa cgagcctgta caacatatcc acttagataa gattttactt ataggtgtga
    12061 cgatggtcgg agtgattgca atggttttat ttgccactgg gatgcttcac gtatagaagt
    12121 ataaaagaag tataaagaga ggttgtgagc ctgtcgttta ttcaaaaaca tactaggaaa
    12181 ggagctgtga cacaaatgtt gtcacagctc ctttttcaga ataaacggtg tttaagagac
    12241 agccaatcgg caataaggct gaacgtctct ttcacagcct cttttttgat gagtcatcat
    12301 acattttttc gcttaaatac gagatatcct aatgctaaga acaaaacgat gtaagctaaa
    12361 ttaccgaata gcatttgatt agtagaaaga tgagtcaatt gcttcatgat ctcttcgttt
    12421 ccaatttggt tttgcagatt caacatactg atcggattcc atttgatcca ctcccatttt
    12481 tggatgagtg caataaggat accagatatg attgacgaag caaaataaaa gacgattcct
    12541 gctgagatgg atgcgccgga actattgata aagcaagcaa tcatcaacac taaacttaaa
    12601 atcagccata aaccgatata actacccaaa gtataagtga acaagctttg gatcagtgtt
    12661 tgaccagtag aaaccttctc cgtgaaggaa atcgaaggga aaagaatcag tttcatcaac
    12721 acagtgacaa taatagctaa aaggtataaa taaacagaat aaataactaa tgtgatccat
    12781 ttgcttacta atatttctcc tcgagaatac tttcgataaa ggagattttt caatgtacca
    12841 tattgtgctt ccatcgaaat gatcgtgctt gcagctgcaa tcatgataaa gacaatccaa
    12901 gatgtggcac taaaaagctg agcagtcatt tcaactggat caatgatcgt cgtgtatttt
    12961 ttggctagaa gtgctgttcc tatgagcaaa acaactaata gaacagataa aacagcagtt
    13021 gactttttct tgatcaattt aaataattct tgttttacaa gcaatgtcat ttttcttctt
    13081 ccccctttac tgaatccaga atttccaata gagaagattc caaatcattc gtcagatgct
    13141 ggacgtctgt cacatggata ttttgtgcag acaatagctg gatcacggtt cctaataatt
    13201 cttcgttttc ttttaaaagc ttgattttat caggatcttc caaagtatat ccagcttctg
    13261 tcaagatcgt tcgagcagct agatcatttg ttgtagagat aacgatgaaa tcttgcgctg
    13321 tttggttcaa ttcagccatt gtacattgtt tgatgatctt tccttgattg atcaggatca
    13381 gctcatcgac cattttttca agctcgctga gtaagtgact ggagatcaag aaagaggtgc
    13441 cctgagtagc caagtcggta atgatttttc gcacatctct tgttgcttgt ggatccaaac
    13501 cgttcatcgg ctcatctaaa ataacgatag acggatggtt cagtaaggca agagcaattc
    13561 ccaatttttg tttcattccc aacgagtagg atttcgcttt tttgtgaatg tacttttcca
    13621 tcttgagctg attcacgata gtgtcgatag cagatacatc gttggtttca gagaataact
    13681 tcagatgatc atagcctgtt aagaatggat agatacccgg gtattcgatc aatgctccta
    13741 cttgttccaa ccctttatga ctagagggag aaatctcatt ttcaccaata cagattgttc
    13801 catctgagta atggatcagt cctaaaatag ctttcatgat cgttgttttt ccagcgccat
    13861 taggacctac taatccaacg attttgccag gggataagct aaaactgaca tcgctgaggg
    13921 cttgtttgtg accaaagttc ttggataatt cttggaccga aagacttttc aaaaatattc
    13981 actccttttc tatagatagt acaagcatac acctgatgag tggagaagtc gctaggtcat
    14041 tggacttatt tcttattttt tcataaaaaa attgaatatt aaataaaaag gcttattttt
    14101 tctgatcagt acttattcca agtgtctagt ggccgctagt aatcttgatg ccaatgatac
    14161 tgatgagtag catccccaca aagaaccaag tggaaagatt cagcttatca tgaaacaaga
    14221 ccacaccaat tatgaccgaa ccaactgcgc caatccctgt ccagatagga taagagagac
    14281 tcatcggcaa acttttgatt gcctttgaca aaaagtaaaa actagcaatc atgccaatca
    14341 ctgtgtaaaa agaataattg attttagaaa aaccatcact ccatttcatc atcgttgacc
    14401 agaatacttc caaaattcct gctatgatta attccatcca tgccatactt acattcctcc
    14461 ttaactataa aaaaatacac aaaaagagga aaatcatccc ttcaaagacc taatggaatg
    14521 atcatcctct ttatcctacc cggtggcaga ttgtatatct ttttctgtag tatagcacac
    14581 ctatccagat atcggcaatc tcatactttt tagtaaagtg taccaagcac tgacggatat
    14641 atcgttagcg gaaaagaaga tgaatgcttt gcaatttcat agaaagaagt tgttatttat
    14701 taatcattta aaaaaaacag taactagacc ttatttctat atattatcgg gcaataaatt
    14761 aaaaaaagct ttaccttaca ataagtaaga taatttttgt gataaaaaaa taacgaaaaa
    14821 tatttaaaaa tagtgtctat ttcgtgagaa aacaactata ttttttatgt gttttgcttt
    14881 ataattaaaa aaatattgtc attttatgga ggaaaatata tggaaaatta tcttagcctt
    14941 ttaggcgtct tgattgttat tctcgggttt gcatttaaat tagactcgat attgatcgta
    15001 atggttgctt tagttgttac agctttgact ggcggtcttg gcattcaagg aatgttagaa
    15061 cttttaggta caagttttgt aaacaatcgc gggatggcga tttttatcat cattatgttg
    15121 gcaacaggaa cgcttgaaag aaatggtctg aaagaatcag cggcttcctt gatcaagcga
    15181 ttcaagaaag tatctgctgg tatgattgta gatatttatg ggatctttcg aatgatcttt
    15241 gcggctttta acgtgagttt tggtggagtt gctggatttg ttcgtccaat cattttaccg
    15301 atggcattgg ggaccatcga atcaaaaggg ttagaagtcc atcctaagca tgaagaagaa
    15361 ctaaaaggta tggcttcagc tatggaaaat gtctgctggt tctttggcca agtactattc
    15421 atcggaggag caggggcgtt gctagtccaa tcaacactga aagatctagg atatgacgta
    15481 acattgggac agcttgcttt agtggaagtt ccagtggcta tcacggcttt agttgtggca
    15541 agtgtctact tctatcttaa agaaaagaaa ttagcgaaga aatattatcc aggaaagggg
    15601 caataagatg gactttttta caaatcctga tattttatta ggcgataaat tactggagat
    15661 cctttatatc gtgatgggac tcgtattgat ttacactgga gtaagaaatt taacagataa
    15721 aaccaatcct catcgttatg ggtcagcctt tttctggacg gtcttagggg tagttattgc
    15781 aggaggaaga tggcttccgg caatcgtgaa cggtggcttg atctttgcga tgaccatacc
    15841 agcaattgct aagaaagtca gcaaaggaga aagccggctt ccatcaaaag aatatatgga
    15901 aaaaatgtca gacaaactag gaatgaaaat ttttattcca gctttgagta tcggtgtctt
    15961 tgcgatcttg tttgctctat ttaccaattt aggtgcttta gtcggcgtgg gcgtcggtgt
    16021 ttttgcagcc atgctaatct tgatgttctt ttctcgagat aatcgaccaa gtacattttt
    16081 agatgatgca gcagatatgc tgggcacagt agggccgtta agcatgttgc caatgctttt
    16141 agcttctcta ggtgcggtat ttacaagcgc tggagttggg acagtgattt ccaatgcagt
    16201 aggtacgatc gttccagaag gaaatgtgaa catcggcatc atcatttacg ctcttggcat
    16261 ggtgatattt acggtaatca tggggaatgc atttgcggcc atcaccgtga tgacggttgg
    16321 gatcggtgca ccattcgtat ttagtcatgg agcgaatcca gcgttaatag gaatggttgc
    16381 attgacttgc ggtttttgtg ggaccctgct gacaccgatg gctgctaact tcaatatcgt
    16441 gcctgtagcg atgctggaga tgaaagacaa atacggagtg atcaaaaacc agttgtttat
    16501 tgcgttattt atgctagttt tccaaattgc ttatatgatt ctatttaaat aatagaagag
    16561 aggggttgtg acagaataat tgtcgcaacc tcttctaaca ttgtagtgga acactatttt
    16621 tttataagaa tatattaagt ttgatctgtt atgttattta aactattgtt tcctttattt
    16681 acgctgttca aggaaaatgg tatgataaaa gataaccata tgttaaatca atcggacgga
    16741 ggaacaacag atgaaagtac tagtaacagg ttttgatcca tttggtgggg acaaagtgaa
    16801 tccagcctat gaagcagtaa aaaaaatgcc agatgaaatc agcggtgctg aaatcatcaa
    16861 agtggaagta ccgaccgtat tcgaaaaaag cagtcaagta gtcaaagaag ccatccaaca
    16921 gcaccaacca gatgtcgtga tctgtgtcgg acaagcaggt gggcggtcag cagtctcgtt
    16981 cgaacgtgtc gcaatcaatc tagcagaagc aagaatccct gataatgaag gaaatcagcc
    17041 attcgatacg gctttagaag aaaacggtcc agccgcttac tttacctcat tgccaatcaa
    17101 agcaatgaca aaaaatgtac aagatcatgg tctaccagcg tacatctctt atacagcagg
    17161 aacatttgtc tgcaatgata tcatgtatcg tttgcttcat ttgatcgaaa cacaattccc
    17221 ggcaattcga ggtgggttca tccacgtgcc attctcaccc gatcaagtga tcgatcgtcc
    17281 agtgggtacg ccttctatgt ctttagaaga cattgcttct tctttgactt atgcagttga
    17341 agcagcagta gaaaataaag aagatatttc cggaaatgcc ggaacaactc actaatttag
    17401 caagaaacac tggctggttg ctaacgttat gttagtagcc agctttttgt aattagaaag
    17461 gaaagaaaca gccgtggaag aatggcaatc cgtctttgaa gaatggtttc caaaagaaat
    17521 cagcaaaagc tatcccatca aaatttctaa acaatacaca agcagtcaac gttgggagat
    17581 ctatgcaaaa ttaacgaaaa aacagcggga actagtcgat aagcaccgcc gatatctaat
    17641 cagctctcgt tttatggaag aacattattt agctgcaaca gattgggtgt tttctgactt
    17701 caagatcaat ccgttttttc gaacaaaacg aagccagcaa aaattgtatt gtgagtgtgg
    17761 tagagaatta aaagtgcaat acatcgttaa atcgccaaaa accggaaaga tcctaaaact
    17821 aggcatcaac cactttgccg atcacctgca tgtctctccg acagtggcag cttcgatcca
    17881 tcagggaatg acaaaagttg atctggcctt agacgaatta ttatggctta agcagaagaa
    17941 tatcgatttt cctgaaggac tatggcaaaa atactgtttt gtcttgtacc aaaatcgtcg
    18001 gatgaaacag ccatatttac cggatataaa attagctcag cggctcgcag aatttcgcca
    18061 agtagaaatg ccgatttaca tagcggatta tcaagcattg gaaaatgaaa tcaagaaaat
    18121 aagtgaacac atcaatgggc agtcaaaaaa gcgacagata aaaaaagaac tatttgacga
    18181 ctttgcagaa gaattagtaa aggatgtaga agaattcttg atcaactatc gcgcattttt
    18241 acgtaaagat tggcaatcca tcgtatatga agaagtgcca gtacatccta atgcttattt
    18301 tgaaacattt atttccgtgt tgagaaagac aaaacgtcaa cgaacaccag aagtcactgc
    18361 tcagatggaa tattttgcga aaaaccaacg attcatccaa ccaaaaatct atctatttat
    18421 ttggaagcaa tattgtcgct atggttttac agaagggttc ttcgacagca ttcctcggat
    18481 agtcagaaat ggttttttaa aagtattgcg taaagaacga gaagctatac aatctgctga
    18541 taagaaagac cgtacagtct caaaagaaaa atggcaacta gtagtgaaag atatccaatc
    18601 tggaaatgtg caagagacaa tcgacaaatg gaaaggaaaa cattatcgtt ttactgaagc
    18661 tcagaaacaa gcgttggaat attatcagaa gttagaagaa tcactgcgtt tcaatgatga
    18721 ggcaagaaag tatctgaagg aattactata aaaaacatac taagattaga ggggaacatc
    18781 attgacgaat g
//
LOCUS       DAYYAO010000254        10087 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.guided.099, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000254 DAYYAO010000000
VERSION     DAYYAO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.guided.099"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..523
                     /locus_tag="ACHARI_003075"
     CDS             353..523
                     /locus_tag="ACHARI_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8444009.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            537..1139
                     /locus_tag="ACHARI_003076"
     CDS             537..1139
                     /locus_tag="ACHARI_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8444010.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="ACHARI_003077"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="ACHARI_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2143..2640
                     /locus_tag="ACHARI_003078"
     CDS             2143..2640
                     /locus_tag="ACHARI_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFM8444011.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2641..3057
                     /locus_tag="ACHARI_003079"
     CDS             2641..3057
                     /locus_tag="ACHARI_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFM8444012.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="ACHARI_003080"
     CDS             3077..4621
                     /locus_tag="ACHARI_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8444013.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="ACHARI_003081"
     CDS             4764..4988
                     /locus_tag="ACHARI_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8444014.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="ACHARI_003082"
     CDS             5003..5872
                     /locus_tag="ACHARI_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8444015.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="ACHARI_003083"
     CDS             5853..6587
                     /locus_tag="ACHARI_003083"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8444016.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="ACHARI_003084"
     CDS             6620..7528
                     /locus_tag="ACHARI_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8444017.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="ACHARI_003085"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="ACHARI_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM8444018.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="ACHARI_003086"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="ACHARI_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8444019.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9230..9361
                     /locus_tag="ACHARI_003087"
                     /pseudo
     CDS             9230..9361
                     /locus_tag="ACHARI_003087"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9437..9930
                     /locus_tag="ACHARI_003088"
                     /pseudo
     CDS             9437..9930
                     /locus_tag="ACHARI_003088"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3548 a         1571 c         2207 g         2761 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ctgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DAYYAO010000255         7958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.guided.113, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000255 DAYYAO010000000
VERSION     DAYYAO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.guided.113"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACHARI_003089"
     CDS             128..1564
                     /locus_tag="ACHARI_003089"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8444020.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACHARI_003090"
     CDS             1753..4200
                     /locus_tag="ACHARI_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8444021.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHARI_003091"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHARI_003091"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8444022.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACHARI_003092"
     CDS             5487..5693
                     /locus_tag="ACHARI_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8444023.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACHARI_003093"
     CDS             complement(5690..6793)
                     /locus_tag="ACHARI_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8444024.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACHARI_003094"
     CDS             7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACHARI_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8444025.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2205 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taatttttta ttttataagt aagataaata atgaaagaat agagatactg
     7261 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
     7321 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
     7381 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
     7441 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
     7501 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
     7561 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
     7621 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
     7681 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
     7741 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
     7801 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
     7861 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
     7921 agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DAYYAO010000256         5497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.guided.135, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000256 DAYYAO010000000
VERSION     DAYYAO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.guided.135"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..1014
                     /locus_tag="ACHARI_003095"
     CDS             322..1014
                     /locus_tag="ACHARI_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8444026.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="ACHARI_003096"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="ACHARI_003096"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8444027.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="ACHARI_003097"
     CDS             complement(1910..3862)
                     /locus_tag="ACHARI_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8444028.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="ACHARI_003098"
     CDS             complement(3869..4105)
                     /locus_tag="ACHARI_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8444029.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="ACHARI_003099"
     CDS             4507..4797
                     /locus_tag="ACHARI_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8444030.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(5069..5418)
                     /locus_tag="ACHARI_003100"
                     /pseudo
     CDS             complement(5069..5418)
                     /locus_tag="ACHARI_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1867 a          889 c          945 g         1796 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataag
//
LOCUS       DAYYAO010000257         4067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.guided.158, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000257 DAYYAO010000000
VERSION     DAYYAO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.guided.158"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1495
                     /gene="msr(C)"
                     /locus_tag="ACHARI_003101"
     CDS             17..1495
                     /gene="msr(C)"
                     /locus_tag="ACHARI_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8444031.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1542..1988
                     /locus_tag="ACHARI_003102"
     CDS             1542..1988
                     /locus_tag="ACHARI_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8444032.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2187..2702)
                     /locus_tag="ACHARI_003103"
     CDS             complement(2187..2702)
                     /locus_tag="ACHARI_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8444033.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2819..4021
                     /locus_tag="ACHARI_003104"
     CDS             2819..4021
                     /locus_tag="ACHARI_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8444034.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1212 a          697 c          886 g         1272 t
ORIGIN      
        1 taggagtgta aaacatatgg aaaatttagc agtaaatata acaaatctac aagtcagctt
       61 tggcaaccag ctagaactat ctattgattc tcttcgtgtc tatcagcaag atcgtatagg
      121 gatcatagga gaaaatggtg tcggtaaatc gactttgctc aaactaatag ccggtgaact
      181 ttttcccgat catgggaaaa tccaaacaga gatcaccttc aactacctgc ctcaattaac
      241 ctatctcgct gaagcaaagg acctaaattt ggaattagcc agtcatttcc agttaagact
      301 ggaagaaact tcagagcgga aatggagcgg aggggaagaa cgaaagatcg agttgatacg
      361 tcttctttct tcttatgaac aagggatgct tctagacgag ccaacaaccc atctagatag
      421 aaaaagtatt gatcgactga ttgaagagct tcgttattat tatggtacgc tggtttttgt
      481 tagtcatgat cgctattttc tagatgagct agcatcgaaa atctgggaag taaaagacgg
      541 agaaatccga gagttttcgg ggaattatag tgcctatctc actcaaaagg aattggagaa
      601 aaagactcag ctacgagaag cagagtcgat catgaaagag aaaaaacgat tggaaaaatc
      661 gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa
      721 gcaacaaatc agaccggatc ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc
      781 catccaaaag aatgcgaaaa cattagagag aagactccaa aaaataggag aaacaaccaa
      841 gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac tccacagccg
      901 ctatcccatc atgggacaaa atgtccaatt ggaaagaagt gggagaacat tactggtaaa
      961 tggtgatttt cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg
     1021 taagacaacc ttattagaac atatccgcaa acaaggagaa ggaatccttc tctctccgaa
     1081 agtaagcttt caagtatatc agcaaaaggg ttatcaaatg acatctgaag aatccatcat
     1141 tcgttttgtc atgagacaaa cagagttttc agaatcgctt gtccgtagtt tgctgaatca
     1201 cttagggttt gctcaggaaa ctttgacgaa accgttatgt acattaagtg ggggagaagc
     1261 gacccgtctg acgattgctt tgctttttac taagccaagt aatgtgttgc tgttagatga
     1321 accgactaat tttattgata tggcaacgat cgaagcttta gagaagctaa tgcaaatata
     1381 tccgggaacg attttgttta cttctcatga ttcttacttt gtcgagcgta cggctgatga
     1441 agtttatgaa ataaaagggc agaaaataaa aaaagtactt acgagaaatt tttaaatgtg
     1501 ctacgatgat tttcgtgaca aatttaaagg agtggtacag attgcctaca aataaaaaag
     1561 aaggaataat ttttacaaca atgatgtgtt ttttgatggt cttggggatg agcgcgtata
     1621 atttatggct gcatcaagat cttcatttat caagtttgtt gattggtcta gtgcctggat
     1681 tcgtggtggc tttcattttg gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct
     1741 ttaaactacc agtaaatcca gaaagcaagg tccaaatgat cttaactatt tcatgcttga
     1801 tggttttagg aatggtgaca tgtatgtctt tattcggtgt gatcatggaa caaggaatac
     1861 ctgatcaatt attctctgct tatatgaatg cttggaaaat gaatgtgatt gccgctttac
     1921 cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt gctaaaagca attcaaagta
     1981 atgaatgatg gaacactgtt atttgaaaaa aggccttggc atgctttttg tcatagtctc
     2041 ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg ttttggcctg tcgttcggat
     2101 cgctttttct tttccaactg tattgctctt ttattgaaga taatttgctc ttcaatagat
     2161 atttctagaa aggttctgtt tatcccttac tcaaaaaact gctgcatttg ttctcttact
     2221 tgctgacacc attggagttc ctgctgacaa agcataattc tgcgctggat cacaacataa
     2281 cgtccaaatt cttgtttctt gtctagcgag aggtcattca attttttttg atttttttgc
     2341 atgattttgt tcagctgttg ttctctagtg aatagcagtc cagtagctgt tgttttatcg
     2401 agcgtcgaaa aagcatagat ttttgctaga aattcatctt tttgcgtagg agcgggagtt
     2461 tcctctttga tccattcctc tacaagagca cgtccttcat cagtcaagtg gtagatcttt
     2521 ttttgagagt gctcgtcttt gtccaaaacg gtcacataac cctgctggtc taacttggca
     2581 agcgttgtat agatctgact gtgatgggct tcccaaaaaa ttgtgatcag ctgcttcaat
     2641 tcatacccag aacgtggttt ggataaaagc acgcataata atatataggc taaagtattc
     2701 ataaaaatcc tacctctcgt cttattttct ttattatatc acagtaaagt ttccacgttt
     2761 tacatgtgca tatttacatg taaaataaat catgtatgat gctaatagag agaaagggat
     2821 ggaaatattg gagaatcaaa tagatataca agaacaaaca aatgaagaaa caaaggggaa
     2881 aagttcagcg ctagttgtag cagctacctg tatgatcgct tttatgggag tgggtctagt
     2941 agacccaatc ttaaaaacga ttgctgcaca gttaaacgct acgccagctg aaacgaccct
     3001 gttatttact agttacatgt tagtcactgg tgtggtgatg ctatttacag gtttcttatc
     3061 tagtcggatc ggtttgaaaa aaacattgat gacaggattg tttatcatcg ttgtattcgc
     3121 tggtcttggc ggattatctg ggaatatcgg aatgctgatt gggttacgtg ccggctgggg
     3181 tgtcgggaat gctttatttg tctctactgc tttagcaacg atcgtcagta ttttagttgg
     3241 taacacagaa aaagcaatca tgatgtatga agcagcactt ggctgcggaa tggctgttgg
     3301 ccctctagtc ggtggggtct tgggcagtgg ttcatggcgt tatccatttt ttggggtagc
     3361 atttttgatg ttttgcggtt ttatagccct agcggtactt ttacctgaaa ctgagaaacc
     3421 aaagagaaaa gtaggccttt gggaaggtgt caaagcgtta agcaatcata aacttcgttc
     3481 agttggacta atcgcattgt tatataattt cggattcttt actttacttg cgtatgcgcc
     3541 atttttattg attggctatt ctgaattaga agtcggtttt gtattttttg gctggggtgt
     3601 gcttctggct attgcttcga ttttcgttgc accggctctt gagcggaagt ttacgactta
     3661 tcggacaatt ttagcagcat ttgtcttatt cattatttgt ttgctgttat taggagctgg
     3721 aacttctatg ccagtccttg taccgatcag cattgtgtta gcaggatttt tccaaggaat
     3781 tatcaataca ctgttgacga cgattgctat ggaaatacca ggtctgcaac gaaacgttgc
     3841 ttcgtccagc tatagttttg tccgcttttt cggtggagct ttagcaccat ttattgcagg
     3901 tgtgattggt gaacatattg gtgaaagcta tgcgttttat tttgcaggtg ccattgttct
     3961 tatcagtgta tgttgtatca tttatcaccg taattatttt gctacaaatg aagaagaata
     4021 agaaatcaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaa
//
LOCUS       DAYYAO010000258         2772 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.guided.174, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000258 DAYYAO010000000
VERSION     DAYYAO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.guided.174"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACHARI_003105"
     CDS             111..194
                     /locus_tag="ACHARI_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8444035.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACHARI_003106"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACHARI_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8444036.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="ACHARI_003107"
     CDS             complement(1319..2182)
                     /locus_tag="ACHARI_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8444037.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="ACHARI_003108"
     CDS             complement(2184..2456)
                     /locus_tag="ACHARI_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8444038.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="ACHARI_003109"
     CDS             complement(2473..2682)
                     /locus_tag="ACHARI_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8444039.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DAYYAO010000259         1269 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5216
            SAMEA115707574-rid22593583.guided.214, whole genome shotgun
            sequence.
ACCESSION   DAYYAO010000259 DAYYAO010000000
VERSION     DAYYAO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707574
            Sequence Read Archive: ERR13197303
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290116.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:17:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707574-rid22593583.guided.214"
                     /isolate="VRE5216"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1143
                     /locus_tag="ACHARI_003110"
                     /note="aph(2'')-Ia"
     CDS             <1..1143
                     /locus_tag="ACHARI_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFM8444040.1"
                     /translation="KGFYSLFKTLQQNRTRYIKLIFEFLKKERNANAVILDPHKNNPR
                     AIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFK
                     VDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPN
                     IEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDI
                     SECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDF
                     SCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNID
                     IEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          543 a          101 c          206 g          419 t
ORIGIN      
        1 aagggatttt attctttatt taaaactttg caacagaacc gtacaagata tattaaattg
       61 atttttgaat ttttgaaaaa agaaagaaat gctaatgcag ttattttaga ccctcataaa
      121 aataatccaa gagcaataag ggcataccaa aaatctggtt ttagaattat tgaagatttg
      181 ccagaacatg aattacacga gggcaaaaaa gaagattgtt atttaatgga atatagatat
      241 gatgataatg ccacaaatgt taaggcaatg aaatatttaa ttgagcatta ctttgataat
      301 ttcaaagtag atagtattga aataatcggt agtggttatg atagtgtggc atatttagtt
      361 aataatgaat acatttttaa aacaaaattt agtactaata agaaaaaagg ttatgcaaaa
      421 gaaaaagcaa tatataattt tttaaataca aatttagaaa ctaatgtaaa aattcctaat
      481 attgaatatt cgtatattag tgatgaatta tctatactag gttataaaga aattaaagga
      541 acttttttaa caccagaaat ttattctact atgtcagaag aagaacaaaa tttgttaaaa
      601 cgagatattg ccagtttttt aagacaaatg cacggtttag attatacaga tattagtgaa
      661 tgtactattg ataataaaca aaatgtatta gaagagtata tattgttgcg tgaaactatt
      721 tataatgatt taactgatat agaaaaagat tatatagaaa gttttatgga aagactaaat
      781 gcaacaacag tttttgaggg taaaaagtgt ttatgccata atgattttag ttgtaatcat
      841 ctattgttag atggcaataa tagattaact ggaataattg attttggaga ttctggaatt
      901 atagatgaat attgtgattt tatatactta cttgaagata gtgaagaaga aataggaaca
      961 aattttggag aagatatatt aagaatgtat ggaaatatag atattgagaa agcaaaagaa
     1021 tatcaagata tagttgaaga atattatcct attgaaacta ttgtttatgg aattaaaaat
     1081 attaaacagg aatttatcga aaatggtaga aaagaaattt ataaaaggac ttataaagat
     1141 tgattatata atatatgaaa agctattata aaagacatta gtattaaata gtttaaaaaa
     1201 atgaaaaata ataaaggaag tgagtcaatt ccagactcct gtgtaaaatg ctatacaatg
     1261 tttttacca
//
